LOCUS       DATMBB010000001         5820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_38597, whole genome shotgun sequence.
ACCESSION   DATMBB010000001 DATMBB010000000
VERSION     DATMBB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38597"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VNL92_00005"
     CDS             complement(<1..1199)
                     /locus_tag="VNL92_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HWO93129.1"
                     /translation="MRELIRRHPAILAVAEVARDDDRGAVVLDVTFRVNLPNAWMARG
                     QSPNGVRAEEIVRFRFKSGFPLRPPTISLRPDFDRNLPHIQPWLEEGRPVPCIFDGPV
                     SELIHHEGLQGVLNQTAVWLDRAALGQLIDPAQGWEPVRRDELDDYLLADAGFLRSQV
                     GRDAGYKFFDLHYIRLTKGTQSFVHGEILETQPNLRSDQGRTAIVPEQAVGDSGRVSA
                     GRSVALVVWPGRLPSGKPVVVDTYMPDTVTNLDELVARAREYGCGEALQSGLSWLRAS
                     VRGRRDDGPFAMAIVVVARRPFNLIGTDSPLEICPYVTDISVPAVFERGGSTVIRPAG
                     HRDRIAPELLRRLAGQGQTEPRAWTLLGCGSLGSKIAMHLARSGAAPSVVVDSRSMSP
                     HNAARHAL"
     gene            complement(1248..2411)
                     /locus_tag="VNL92_00010"
     CDS             complement(1248..2411)
                     /locus_tag="VNL92_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019488184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HWO93130.1"
                     /translation="MAARISFFPVGNGDMTLIQLESGRTILIDLNIRAAADDPEDSTP
                     DVAKMLRERLSRDARGRLYVDVFLLSHPDQDHCSGLRKHFHLGAPADWSKSEDKILIR
                     EVWSSPIVFRRASRNHVLCDDAQAFNTEARRRVKRFRDSAGGVEDGDRILILGEDENG
                     KTDDLGSILVKVDGLFSTVNGVQDGSLSVRLLGPLPKSDDEDEEEVLSKNESSTILRF
                     SLTGDGQADAGRFMTGGDAEVAIWERLWDRHKKRPDWLSYDILLSPHHCSWHSLSYDS
                     WSEMGEDAQVCEKARKALAQTREGAVIVSSSKPIKDDDADPPCIRAKREYVAITKAAK
                     GTFKCVGELPSEDEPDVLEFEVGRDGPRPKTRLMKAASVLGAGVVGRQPLAHG"
     gene            complement(2475..3137)
                     /locus_tag="VNL92_00015"
     CDS             complement(2475..3137)
                     /locus_tag="VNL92_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93131.1"
                     /translation="MTRIPFATYDFFGYLASGFLLVIGMDVALGFPRVLGQNLTPVEI
                     VALLLAVYVAGQLIATPSKAVLEDGLVEKILGRPNVNLFREKRPMLRGLLFPGFYKPL
                     PDAVRERVLSRAGREGIADPGEALFLHVRYSPEVRTDEKLLGRLDTFLNLYGFARNLS
                     FASLVVGAGLAARTGWPGANPALRQYAATALAVGLLLLYRYLKFFRQYSYELFNTYAR
                     SK"
     gene            complement(3148..3669)
                     /locus_tag="VNL92_00020"
     CDS             complement(3148..3669)
                     /locus_tag="VNL92_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93132.1"
                     /translation="MERAADGRLLVVADSSFLINFLAVDRMDILGRLPQFRFHVVNHV
                     LAEVRYEVQRRRLHAALEAGTVTEIEITEPAEILLYDEFRHFLGDGEAASLAVAIRRR
                     WVIAADEKGAFRRELFGRLGDDYLLDTLGALVTAIKADVITVADAETVRAQMREHRFE
                     MESTPFEELLREE"
     gene            complement(3673..4944)
                     /locus_tag="VNL92_00025"
     CDS             complement(3673..4944)
                     /locus_tag="VNL92_00025"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWO93133.1"
                     /translation="MPLDKEELGRRLKKAREAVGLTQDDAAEQLGISRGALAQFEMGA
                     KAPNSLHLVRLAEIYRRDLAELLAEQFDEARRDPLTALFRADLQLASDRERASAVAEC
                     AKLCREYTNLEILLGIDEDRVYDVNYNTPPMRTRWDAIRQGERLAELERARLSLGNDP
                     LTNVPELLEAQGVRFIEMPFPETISGLFLSDARYGPSIIVNEEHGAPRRRFSSAHEYC
                     HLLVDRDLGGLVSRAENREELPEVRANAFAAAFLMPDEGVRAFVRRLGKGEQSRSQLR
                     AYDETAVVEGQRRREAHSQDIQVYDVAHLAHHFGVSYETALYRLLNLKLVTEEERARL
                     IQQKETATRIMRFLGPVPDAKTHGRKPFRHQLAVLAVEALRREVISRAKLKELCELAQ
                     VQPAEFETLVAMVKQEPQKPSKGRRAYVPRA"
     gene            5064..>5820
                     /locus_tag="VNL92_00030"
     CDS             5064..>5820
                     /locus_tag="VNL92_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93134.1"
                     /translation="MRIAPGEKFACHAFTRCWLADGVPPEIELRPRLWAATSLDFDVA
                     EHWRNWLGSITVDELREANLIIYTTMASSRPEILDDENATLAKVLDYVLFGLLLQGIP
                     VYNRAYSLNGAHVRGEINVRQFSDLRGYQPTHELVEFRPGVNELRRAEALSVRLQRVN
                     EGGADWGRLRRGVNALLRGSRMDDGTERLHQFVRALEALIKPDIGNTRRQFAHRIDQT
                     FTVANAETRETLNQIFDLRSRIEHVHDPLEVLAG"
BASE COUNT          993 a         1983 c         1840 g         1004 t
ORIGIN      
        1 agtgcatggc gggcggcgtt atgcggactc atggagcggc tgtcgacgac gacgcttgga
       61 gccgcccctg agcgggcaag gtgcattgcg atcttcgatc ccaggctgcc gcagcccagg
      121 agcgtccatg cccgaggctc ggtctgcccc tggccggcga gccgccgaag caattccggc
      181 gcgatccgat cgcggtgccc ggcgggacgg atgacagtac tgcccccgcg ctcgaagacc
      241 gccggcactg aaatgtcggt gacgtacgga cagatctcga gcgggctgtc ggtcccgatc
      301 aggttgaatg ggcggcgcgc gacgacgacg atcgccattg caaacggccc atcgtcgcgc
      361 cggcctctga cactcgcccg gagccacgac agtccgctct gaagcgcctc gccgcagccg
      421 tattcacgcg cacgcgcaac cagctcgtcg aggttcgtga ccgtgtccgg catgtacgta
      481 tcgaccacaa ccggcttgcc cgaaggcaac ctgcccggcc ataccacgag agcgaccgat
      541 cgtcccgcgg agacacgccc actgtccccg acggcctgct ccggcacgat cgcggtgcgt
      601 ccctggtcgg atcgcaggtt cggctgtgtc tcgaggatct ctccgtgaac gaagctctgc
      661 gtgcctttcg tcaagcggat gtagtgaaga tcgaagaatt tgtagcctgc gtcccgtcca
      721 acttgggacc gcagaaatcc ggcgtccgcg agcagataat cgtccagctc gtcacgacga
      781 actggctccc agccttgcgc gggatcgatc agctgcccga gcgcggcccg gtcaagccag
      841 acggcggtct gattcagaac accctggagc ccctcgtggt ggatcagctc ggagaccggg
      901 ccatcgaaga tgcacggcac cgggcggcct tcttccagcc agggctggat atggggaaga
      961 tttcgatcga agtccggtcg gagcgagatc gtcggtgggc gaagagggaa cccggacttg
     1021 aatctgaagc gcacgatctc ctctgcgcgt acgccgttcg gactctgacc gcgcgccatc
     1081 cacgcgttcg ggagattgac gcggaacgtg acgtcgagga cgaccgcccc gcggtcatcg
     1141 tcgcgagcga cctcggcgac agccaggatc gcagggtggc gccggatgag ttccctcacc
     1201 cgcgcaagta catccggcgg cagcgcgggc gccaggctcg ttactcctta cccgtgcgcg
     1261 agaggctgcc ggccgacgac gccggcgccc aggacggacg ccgccttcat gagccgcgtc
     1321 ttcgggcgtg gcccgtcccg cccgacttcg aactccagga cgtcgggctc atcctccgag
     1381 ggcaactcgc cgacgcactt gaacgtgccc ttggcggcct tggtgatcgc cacgtactcg
     1441 cgcttggcac ggatgcaggg cgggtccgca tcgtcgtcct ttatcggctt gctgctggag
     1501 acgatcaccg cgccttctcg tgtttgcgcc aacgccttgc gggccttttc gcacacctgt
     1561 gcgtcctcgc ccatctcgct ccagctgtcg taggagaggg agtgccacga gcagtggtgg
     1621 ggcgagagca ggatgtcgta ggaaagccag tccggccgct tcttgtggcg gtcccacagg
     1681 cgctcccaga tcgcgacctc ggcatcgccc ccggtcatga aacggcccgc atccgcctgc
     1741 ccgtcgcccg tgagcgagaa gcgaagaatg gtgctcgact cgttcttcga gagcacttcc
     1801 tcctcatctt cgtcgtcgct cttcggcagc ggcccgagca gtctcaccga cagcgatccg
     1861 tcctggaccc cgttcaccgt cgagaagagt ccatcgacct tcaccaggat gctgcccagg
     1921 tcatcggtct tgccgttctc gtcctctccg aggatcagaa tccgatcgcc gtcctccacc
     1981 ccgccagcgc tgtcgcggaa tcgcttcacg cggcgcctcg cctcagtgtt gaacgcctgg
     2041 gcatcgtcgc agagcacatg gttcctcgac gcccgccgga acacgatcgg ggaagaccag
     2101 acctcacgga tgagaatctt gtcctcagac ttcgaccagt cggccggcgc tccgagatga
     2161 aagtgcttac gcagcccaga acagtggtcc tggtcgggat ggctaagcag aaagacgtcc
     2221 acgtagagcc gccccctggc atctcggctc aggcgctcgc gcagcatctt tgcgacgtcc
     2281 ggcgtcgagt cttcaggatc atcggcggca gctcgaatat tgagatcgat caggatggtc
     2341 cggccgctct cgagctgaat gagcgtcatg tcgccattgc ctacagggaa aaatgagatc
     2401 ctggctgcca ttagctgttc ctctcctttc gatcgctcga tccgtgaagc accgtgtcgc
     2461 tgacgaaact cggttcattt gctccttgca taggtgttga acagctcgta cgagtactgc
     2521 cggaagaact tgaggtaccg gtagagcagc aacaggccga cggcgagcgc ggtcgcagca
     2581 tattgccgga gcgcgggatt cgcgccgggc caccccgtcc gcgcggcgag cccggcaccg
     2641 accaccagac tcgcgaacga gaggttccgg gcgaacccgt agagattgag aaacgtgtcg
     2701 aggcgtccga gcagcttctc gtcggtgcga acttcgggac tgtaccgaac atgtaggaag
     2761 agcgcctctc cggggtcggc gataccttca cggccggcgc ggctcagcac ccgctccctg
     2821 acggcgtcgg gcagcggctt gtagaagccg gggaacagta ggcctcggag catcgggcgc
     2881 ttctcgcgaa agaggttgac gttcggacgg ccgaggatct tctcgacgag accgtcctcg
     2941 agcacggcct tgctcggcgt cgcgatgagc tgcccggcca cgtagacggc caagagcagc
     3001 gccacgatct cgactggtgt gaggttctgg ccgaggaccc ggggaaaccc gagcgccaca
     3061 tccatcccga tgacgaggag gaaaccagaa gcgaggtacc cgaagaagtc gtaggtggca
     3121 aaggggatcc gcgtcattcc cggacgatca ctcctccctc agcagctcct cgaacggcgt
     3181 ggattccatc tcgaaccggt gctcccgcat ctgcgcacgc accgtctcgg cgtccgcgac
     3241 cgtgatgacg tccgccttga tcgccgtgac cagcgcgccc agcgtgtcga gcaagtagtc
     3301 gtccccgagt cggccgaaca gctcgcgccg gaacgcccct ttctcgtcgg cggcgatgac
     3361 ccagcgccgc cggatggcca cggccagcga ggccgcctcg ccatccccga gaaagtgccg
     3421 gaactcgtcg tacagcagaa tctcggccgg ctcggtgatc tcgatctcgg tcaccgtccc
     3481 ggcctcgagc gcggcgtgca gccgacggcg ctgaacctcg taccggacct cggccaggac
     3541 gtgattgacc acgtggaacc ggaactgcgg gaggcggccg aggatgtcca tccggtccac
     3601 ggcgagaaag ttgatgagaa agcttgagtc cgccacgacg agcaggcgac catcggccgc
     3661 tcgttccacg cctcacgctc gcgggacata ggcccgtcgc cccttcgatg gtttttgcgg
     3721 ctcctgcttc accatcgcga ccagggtctc gaactcggcg ggctggacct gtgccagctc
     3781 gcagagctcc ttcagcttcg cgcggctgat gacctcccgg cgaagcgcct cgacggcgag
     3841 cactgcgagc tgatgccgaa acggcttgcg tccgtgcgtc ttggcgtctg gaacagggcc
     3901 aaggaagcgc atgatcctgg tcgccgtctc cttctgctgg atcagtcggg cccgctcttc
     3961 ctccgtcacg agcttgaggt tcagcaaccg gtagagcgcc gtctcgtagc tcacgccgaa
     4021 gtgatgggcg agatgcgcca cgtcgtacac ctggatgtcc tgcgagtggg cctcgcggcg
     4081 ccgttgccct tcgactactg cggtctcgtc gtatgcccgg agctggctcc ggctctgctc
     4141 gcccttgccc agccggcgga caaacgcgcg gacgccttcg tccggcatca ggaacgccgc
     4201 cgcgaacgcg ttggcccgca cctctggtag ttcttctcgg ttctccgccc ggctgacgag
     4261 tccgccgaga tcgcggtcaa cgagcagatg acagtactca tgggcagacg agaaccggcg
     4321 ccgtggcgcc ccgtgctcct cgttcacgat gatcgacggc ccgtaccgcg cgtcgctcag
     4381 gaacagacca gagatcgttt cggggaatgg catctcgatg aatcggacgc cctgggcttc
     4441 caggagttcg gggacgttcg tcagcggatc gtttccgagg ctcaggcgcg cccgctccag
     4501 ctccgccagg cgctcgccct ggcgaatggc atcccagcgc gtccgcatcg gcggggtgtt
     4561 gtagttcacg tcgtagacgc ggtcctcatc gatgccgagc aggatctcga ggttcgtgta
     4621 ctcacggcaa agcttcgcgc actcggcaac cgcgctcgcc cgttcgcggt cgctcgcgag
     4681 ttggaggtcg gcccggaaca gcgccgtcag cgggtcgcgc ctggcctcgt cgaactgctc
     4741 ggcgagcaac tcggcaaggt cgcgccggta gatctcggca agccggacga ggtgtaggct
     4801 gtttggcgcc ttggcgccca tctcgaactg tgcgagagcg ccgcgcgaga ttccgagctg
     4861 ttcggcggcg tcgtcctggg tgagcccgac cgcctccctg gccttcttca gacgacggcc
     4921 cagctcctcc ttgtccaggg gcacgcgcgt cacctccttc tgttggtaac acattatgtc
     4981 ataattctgc tcatagagct accgccctgc gagccggtcc ggcggcccga gccccagcag
     5041 ggcaactgat ctggaggctc ccgatgcgaa tcgcacctgg cgagaagttc gcctgccacg
     5101 cgttcacccg atgctggctc gcggacggcg tgccgcccga gatcgagctt cggccgcgac
     5161 tctgggcagc aacgtccctc gacttcgacg tggccgagca ctggaggaac tggctcggct
     5221 ccatcaccgt ggacgagctc cgcgaggcga acctcatcat ctacacgacc atggcgtcgt
     5281 cacgccccga gatcctcgac gacgagaacg ccaccctcgc gaaggtcctc gactacgtcc
     5341 tgttcggcct cctgctccag ggcatccccg tatacaaccg ggcatactcg ctgaacggcg
     5401 cccacgttcg cggcgagatc aacgttcggc agttctccga cctcaggggc taccagccga
     5461 cccacgagct cgtcgagttc aggcccggcg tcaacgagct ccgccgcgcc gaggcgcttt
     5521 ccgtccggct ccagcgcgtg aacgagggcg gcgccgactg gggacgcctt cgacgcggcg
     5581 tcaacgctct gctccgaggc agccggatgg acgatggcac cgagcggctt caccagttcg
     5641 tcagggccct cgaagccctg atcaagcccg acatcggaaa cacgcgacgg cagttcgccc
     5701 atcggatcga ccagaccttc acggtggcga acgccgagac gcgtgagacg ctgaaccaga
     5761 tcttcgatct tcgcagccgt atcgagcacg tccacgaccc gctcgaggtc cttgccggag
//
LOCUS       DATMBB010000002         3186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_38546, whole genome shotgun sequence.
ACCESSION   DATMBB010000002 DATMBB010000000
VERSION     DATMBB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38546"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VNL92_00035"
     CDS             complement(<1..413)
                     /locus_tag="VNL92_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93135.1"
                     /translation="MLTLGRRVASGLGVDLDWLIVGPAPADAADVGSRYVVAHIDLAS
                     SIPAEFSPDTYVAALADYAKQATPCAMLFLQTYEARLVAPRLAARLGSAVVMNAVDAN
                     VDGGSIAVEATAYGGDTRVVYRCGEARLSVIGVTAG"
     gene            complement(505..1305)
                     /locus_tag="VNL92_00040"
     CDS             complement(505..1305)
                     /locus_tag="VNL92_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWO93136.1"
                     /translation="MRIVICAKEVLDPDAVNAYAVAGRLQIAEDGRTVALSAIPSLMN
                     AYDEQAIEAALRIKEAGVECTITVVAVGSESSQHLKRAAALGVDELAIVPLDLANADG
                     LVVGNVLAAFIRSTGGADLVLCGRQASDDDQGVVPPVIGELLGFPIVTMARDISVNDG
                     AVRVVRVTPNGDETVEAALPAVVTVSSELGVPRFPTAAGMMAARKMKPRIVPLAELAL
                     DEGALAPRAKVERMFVPTVQGNCEFIDGSAEEVAQRLIERLRSDGVLP"
     gene            complement(1359..1664)
                     /locus_tag="VNL92_00045"
     CDS             complement(1359..1664)
                     /locus_tag="VNL92_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93137.1"
                     /translation="MTSGERNGDVASSNMVRYGFDEQVAGKYAECDGGSVSADGVAAR
                     EDFAGILTGEYMEQGDPAWRWYHMVHLTKKPPGHVWDSVWCEQGFLFVEDEPRSGDR"
     gene            complement(1680..2804)
                     /locus_tag="VNL92_00050"
     CDS             complement(1680..2804)
                     /locus_tag="VNL92_00050"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWO93138.1"
                     /translation="MTRDPLHTKLCDLFGVEYPILAFTHCKDVAAAVTNAGGIGVLGE
                     LTHTPDEIAQDIRWLKEKCGDKPFGIDLVFPASVPPSANIEDLQTQIPDEHRRFVEDI
                     MRRHNLPAPKLEAGQQPGFGIARGGLLSQEHGRRQLDVVLEERVPLFASGLGNPAFIV
                     DAAHERGIKVAGLIGKVRQARRELEAGVDVIIAQGYDAGGHTGEIGTFTLIPRVVEIA
                     GDTPVLCAGGVGTGQHLAAALCLGASGVWTGTLWLTSRESDMDIVIKEKLIAATEEDT
                     TRSRSLTGKPARLLKTALTDEWEQPGAPAPLPMPLQGILTAELQMSVHQNRYEPFLGT
                     PAGQVIGTMHEMKPCREIVFDLVTEARDVLEGLLGDPVEV"
BASE COUNT          543 a         1100 c         1002 g          541 t
ORIGIN      
        1 cccgccgtga cgccgatgac actcagcctc gcttcgccgc accggtagac cacgcgcgtg
       61 tcgccgccat aggccgtcgc ctcgaccgcg atggacccac catcgacatt cgcatcgacg
      121 gcgttcatca cgacggcgct ccctaagcgg gcggcaaggc gaggcgccac caggcgcgcc
      181 tcgtaggtct gcagaaacag catggcgcat ggggtcgctt gcttcgcata gtcggccagt
      241 gccgcgacgt aggtatcggg cgaaaactcc gccgggatgc tcgatgcgag gtcaatatga
      301 gcgaccacgt aacgcgagcc gacatcggcc gcatccgcgg gggcgggccc gacgatgagc
      361 caatcgaggt cgacaccaag cccggacgcg acgcggcggc ccagggtcaa cacttcctgg
      421 gtgccctcgc taaccgaggt tgcgtcaccc caactacata caacgacagc tgagcccata
      481 tcctataatc ctcttctact gcgcttaggg taacacgccg tccgagcgga gcctctcgat
      541 gagcctctgc gccacttcct cggccgagcc gtcaatgaac tcgcagttcc cctgcaccgt
      601 cggtacgaac attcgctcaa ctttcgcccg cggggccagc gccccttcgt cgagagcgag
      661 ctcagcaagc ggtacgatgc gcggcttcat cttccgtgcc gccatcatgc cggcggcagt
      721 cgggaagcgc ggcacgccca gctcgctgct gacggtgacg accgccggca acgccgcctc
      781 gaccgtctcg tcgccgttag gcgtcacgcg aacgacgcgc acggcgccat cgttcacgct
      841 gatatcgcgc gccatcgtca cgatggggaa gccaagcaat tcgccgatga ccggcggtac
      901 gacgccttgg tcgtcatcgg aagcctgacg gccgcagagc acgagatcgg cgccgcccgt
      961 ggagcgaatg aaggcggcga ggacgttccc cacgacgaga ccatccgcat tcgccaagtc
     1021 gagcggcacg atggcaagct catccacgcc gagggcggcg gctcgcttca ggtgctgcga
     1081 cgactcgcta cccacggcta cgaccgtaat cgtgcactcg acccccgcct ccttaatgcg
     1141 caaggccgcc tcgatggcct gctcgtcgta cgcgttcatc aggcttggga tggccgagag
     1201 ggccacggtg cgcccatctt cggcgatttg aagccgtcca gcgacggcat aggcgttaac
     1261 ggcgtccggg tcgaggactt ccttagcgca gatgacaatt ctcatgctcg tctcgtctca
     1321 ctcttgctca cgcccgggcg tccggcggtc atgactcgtc accgatcgcc ggacctcggt
     1381 tcgtcctcaa cgaagaggaa gccttgctcg caccaaacgc tgtcccacac gtgaccaggc
     1441 ggcttcttcg tcaggtgcac catgtggtac cagcgccagg cggggtcgcc ttgttccatg
     1501 tactcgccgg tcaggatccc agcgaaatcc tctcgcgccg ccacgccatc cgccgatacc
     1561 gaaccaccgt cgcactccgc atacttgccc gctacctgct cgtcgaagcc ataccggacc
     1621 atgttcgagg aggcgacgtc gccgttgcgc tcgccgctcg tcatcctcgc gtccctccgt
     1681 tagacctcga caggatcgcc aagaagcccc tcaaggacat cgcgcgcctc cgtgacgagg
     1741 tcgaacacga tctcccggca cggcttcatc tcgtgcatgg tgccgatcac ttggcccgcc
     1801 ggtgtgccca ggaagggctc gtagcggttc tggtgtacgg acatctgcag ctcggcggtg
     1861 aggatgccct gcagcggcat gggcagcggg gccggagcgc cgggctgctc ccactcgtcc
     1921 gtgagcgccg tcttcaggag gcgcgccggc ttgccggtga ggctgcgcga gcgggtcgtg
     1981 tcttcttccg tggcagcgat gagcttctcc ttgatcacga tgtccatgtc ggattcgcgc
     2041 gatgtcagcc acagcgtgcc cgtccacacg ccggacgctc cgaggcagag ggccgccgcg
     2101 aggtgctgcc ccgtgccaac gccaccggca cacagcacgg gcgtatcgcc tgcgatttcg
     2161 acgacgcgcg ggatgagcgt gaacgtgccg atctcgccgg tatggccgcc ggcatcgtat
     2221 ccctgcgcga tgatgacatc gacgccggcc tcgagctcgc gccgcgcctg gcggaccttg
     2281 ccgatgaggc ctgccacctt gatgccacgc tcgtgcgccg catcgacgat gaaggcgggg
     2341 ttgccgagcc cggaggcgaa gagcggaacg cgctcctcga gcacgacatc gagctgacgc
     2401 cggccgtgct cctggctcag gagcccgccg cgagcgatgc cgaagcccgg ctgctggccg
     2461 gcctccagct tcggcgccgg caggttgtgc cgccgcatga tgtcctcgac gaagcgccgg
     2521 tgctcatcgg gaatctgcgt ctgcaggtcc tcgatgttcg cggagggcgg caccgaggcc
     2581 gggaacacga ggtcgatgcc gaagggcttg tcgccgcact tctccttcag ccagcggatg
     2641 tcctgggcaa tctcatccgg cgtgtgcgta agctcgccga gcacgccgat gccacccgca
     2701 ttggtgaccg ccgcggcgac gtccttgcag tgggtgaacg ccagaatcgg atactcgacc
     2761 ccgaacaggt cgcagagctt cgtgtgaagc ggatctcggg tcatgatggc ctcctcaaaa
     2821 cgcaactgcg caatctcgat cgcgacgaag tcgccggccg tacggcgaca ctcctaccag
     2881 cgtcgaagtt tacatcattc gcgagcgatc tgggagtgca acgtgcggac agacctcacc
     2941 ccctggcccc ctctccatcg gttatcgatg gagaggggga acgcggcagc tggggggtga
     3001 ggtccgatgc cactaggtgg cgctacccaa ggttggcgtt gaggccgttg tcggtctcgt
     3061 cgaactcgat gacagcgttc cccgggtcca cgatgagcgc gatgacatct gggttatcgt
     3121 tggctgcgag gccgtgcaac cgtacactcg ctccttgacc gggatcgagt ccgggcacca
     3181 tgcacg
//
LOCUS       DATMBB010000003         3975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_512070, whole genome shotgun sequence.
ACCESSION   DATMBB010000003 DATMBB010000000
VERSION     DATMBB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512070"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..777)
                     /locus_tag="VNL92_00055"
     CDS             complement(127..777)
                     /locus_tag="VNL92_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93139.1"
                     /translation="MLRVALASVVALVVLPIVAVAGSGEAPLGDRPVALVEVTPNEPW
                     HAGLNAVVVQGPGSASDIVVTIEALSGLQVDALWALFNGAWVYLLPDVPAIEGGFAPA
                     NEARGADSLAAMAVLRQRSIGVTYHVRVYFNETVTQPDLDATAAFFRGFDPGADMLIL
                     ETFPPIGGVDVTTNAPAFCESVRGVLGAKPYVRSVECGEALPPPVPLEPDMPVTSG"
     gene            935..2008
                     /locus_tag="VNL92_00060"
     CDS             935..2008
                     /locus_tag="VNL92_00060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO93140.1"
                     /translation="MKVSVFSLMQRPGDRSDAEAIGAELDTLSMAEDLGYYGAYVAEH
                     HFSPYCLGASPPVLLGYLAAKTRRIRLGMAVSVLPLNNPIKIAEDYATVDVLSGGRLE
                     LGVGRGYNLFEFGSFGVDIAENSERFFEVLEAVRLAWTEDRFSYRGRHYCIENTEVLP
                     KPIQKPHPPVFYANISPGSVEKAAQAGLGICILAGVPPDQIIERRSAWIDAARAAGYD
                     EAFIQHTLDTTPQQKIVWVDEDNGRAREYAAKLISDYGESLEKYAFPGTAYPYRPPGM
                     PQYEMQGPSTRTTSMWSLEAMEATQGAFVGSPEVVTEKLLAYREKAPLEYMLMWCTLG
                     GPDRESLQRCVRLFGEKVLPKLA"
     gene            complement(2016..>3975)
                     /locus_tag="VNL92_00065"
     CDS             complement(2016..>3975)
                     /locus_tag="VNL92_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWO93141.1"
                     /translation="YVRVSLDEERPFRRTSPTHWEATLAAERPQTVAGRAWAATRRYL
                     FGPPLASSRSLEERLSKVKALAVFSSDALSSSAYATEEILLVLVLAGSGNLGPSLPIS
                     LAIVVLLLTVVASYRQAIRAYPQGGGAYSVSRENLGIGAALLAAAALLTDYVLTVAVS
                     ISAGVAAVVSAVPDLGDYRVEMAVVFVALLTIVNLRGLRESGTIFAVPTYLFVLAFGA
                     MIVTGAMRLAFGDQAGSLADPAEPAEFVPGSQELTLFLVLRAFSSGAVALTGVEAMAN
                     GVQAFKPPEAKNAATTLVWMGAILAFFFVGSAFLANRFGIVPIEDETVISQVGRVAFG
                     GENVFYYVLQGATALILVLAANTAFNGFPILASLLAKDGFMPHQFAFRGDRLAYSYGI
                     VVLAAVAAGLLVAFGADTHRLIPLYAVGVFMAFTLSQFGLVLRWRRVREPGWRGAATI
                     NGVGAAATGLVMVIVAVTKFTHGAWFVLVLMPIIVMVLSSIHGHYETVRRELSLDDPA
                     APPLPPPTLPPDRPVIVPVGEPNRAVMRAVRYARGLTSNAMAVHVVTEEGEDVSGLEE
                     RWEELFPDVPLIILESPYRSFQAPFIAFIDRMDIPPNVPLTVVLPEFVPAHWWQNFLH
                     NQTARRLRSALRSRPNTVVVDVPEQLTK"
BASE COUNT          770 a         1328 c         1301 g          576 t
ORIGIN      
        1 caagccaacc gttgcctccg cccgccccaa tgggcccggc gcaacccagg ccgcgcgacg
       61 cgccaccacc tcgggcgccg ttcggtcggc caccgccggg cgggccctag gcttatgcct
      121 cggacgtcag ccgctcgtca ccggcatgtc cggctcaagc gggaccggcg gggggagcgc
      181 ctcgccgcat tccaccgatc ggacgtaggg cttggccccc agcacgccgc gaaccgactc
      241 gcagaacgcc ggagcgttcg tcgtcacgtc cacaccgccg atgggcggaa acgtctcgag
      301 gatgagcatg tccgctccgg gatcgaaccc gcggaagaac gccgccgtcg cgtccaggtc
      361 gggctgcgtg accgtctcgt tgaagtagac gcgaacatgg taggtcacgc ctatcgaccg
      421 ctgccgcaga acggccatcg cggccagcga atcggcgccg cgcgcctcgt tcgccggggc
      481 gaacccaccc tcgattgccg gaacatcggg caagaggtag acccaagctc cgttgaacag
      541 cgcccagagc gcgtcgacct gcagtcccga aagcgcctcg atcgtcacga cgatgtccga
      601 ggcgctgccc ggtccctgga cgacgaccgc gttgaggcct gcgtgccacg gctcgttcgg
      661 cgtgacctcc acgagagcca ccggacgatc gcctagcggc gcctcgccgc tccccgcgac
      721 ggcgacgata ggcaggacca cgagggccac gacggaggcg agtgcgactc ggagcatcgg
      781 cgagccccca atccacgaga tacgcgcgag catagcccac gacggcggtt ggcgccacag
      841 tcatcggctg gggccaaaca ggtggttgtc caaacaatcc gctgtgccct atgatgcgct
      901 cgccgtcggc tctttcggcc gtgaggaggt caagatgaag gtttccgtgt tctccctgat
      961 gcagcgtccc ggcgaccgta gtgatgccga ggcaatcggc gctgagctcg acacactctc
     1021 catggcggag gacctcgggt actacggcgc gtatgtggcg gagcaccact tcagcccata
     1081 ctgccttgga gcatcgccgc ccgtcctgct cggctatctc gccgcgaaga cgcgacgcat
     1141 ccggctcggc atggcggtca gtgtgctgcc actgaacaac ccgatcaaga tcgccgagga
     1201 ctacgccacc gtcgatgtcc tcagcggggg gcggctcgag cttggcgtgg gccgcggcta
     1261 caacctcttc gagtttggat cgttcggcgt ggacatcgcg gagaactcgg agcgcttctt
     1321 cgaggtgctc gaggccgtcc gtctcgcctg gacggaggac cgcttttcgt accgcggacg
     1381 gcactactgc atcgagaata cggaggtgct cccaaagccc attcagaagc cgcacccgcc
     1441 cgtgttctac gcgaacatct cgcccgggtc cgtcgagaag gctgcccaag ccggcctcgg
     1501 catctgcatc ctggctggcg taccgcccga ccagatcatc gagcgacgaa gcgcctggat
     1561 cgatgcggcc cgcgcggcgg gatacgacga ggccttcatc cagcacacgc tcgataccac
     1621 gccgcagcag aagatcgtgt gggtggacga agacaacggc cgcgcccgcg agtatgcggc
     1681 aaagctcatc agcgactacg gcgagtcgtt ggagaagtac gccttccctg gcaccgcgta
     1741 cccctatcgc ccgccgggca tgccgcagta cgagatgcag ggaccttcga cccgaaccac
     1801 gtccatgtgg agcctggagg cgatggaggc cactcagggg gccttcgtcg gttcgcccga
     1861 ggtggtgacc gagaagctac tggcctaccg cgagaaggcg ccgctggagt acatgctgat
     1921 gtggtgcacg ctcggcggcc ccgaccgcga gagtctgcaa cgctgcgtcc ggctgttcgg
     1981 cgagaaggta ctaccgaagc tggcctgagg caccgtcatt tggtcaattg ctccggcacg
     2041 tccacgacaa cggtattcgg cctcgaccgg agcgcggagc gcaagcggcg tgccgtctga
     2101 ttgtggagga agttctgcca ccagtgggct ggcacgaact ccggcagaac aaccgtcagc
     2161 ggcacgtttg gaggaatatc catccggtcg atgaaggcga tgaagggcgc ctggaacgac
     2221 cggtacggcg attccaaaat gatcagcggc acatccggga acagctcctc ccagcgctcc
     2281 tcgagaccgc tcacgtcctc accttcctcc gtaacgacgt gaacagccat cgcgttcgac
     2341 gttagtcctc gggcataccg aaccgcgcgc atcacggccc ggttgggttc cccgacagga
     2401 acgatgaccg ggcggtcggg cggcagcgtt ggcggcggga ggggtggcgc cgccggatcg
     2461 tcgagcgaga gttcgcgtcg cacggtctcg tagtggccat ggatgctgga caagaccatc
     2521 acgataatcg gcatgagcac gagcacgaac caggcgccgt gcgtgaactt tgttactgcc
     2581 acgatgacca tgacgagacc tgtcgcggct gcgccgacac cgttgatcgt agcggcgccc
     2641 cgccagcccg gctccctgac tcgacgccac cgcagtacga gtccgaactg cgacagcgtg
     2701 aacgccatga agacgccgac ggcgtacaag gggatgaggc ggtgcgtatc ggcgccgaaa
     2761 gcgacgagga gcccggcggc aacggccgcg agtacgacaa ttccgtagga gtacgcaagg
     2821 cggtcgccgc ggaacgcgaa ctgatgcggc atgaacccat ccttcgccaa gagcgaggcg
     2881 aggataggaa agccattgaa cgccgtgttc gcagcgagaa ccaagatgag cgcagtcgcc
     2941 ccctgcagaa cgtagtagaa gacgttctcg ccgccgaatg ccacgcgacc tacctgcgag
     3001 atgaccgtct catcctcaat cgggacgatg ccgaagcggt tcgccaggaa cgccgatccg
     3061 acgaagaaga acgccagaat cgcgcccatc caaacaagcg tcgtggctgc attcttggcc
     3121 tccgggggct tgaacgcctg caccccattc gccatcgcct cgactcccgt gagggccacg
     3181 gcgcccgagg aaaacgcccg aagcacgagg aagagcgtca gctcctgcga tccgggcacg
     3241 aattccgccg gctccgcggg atcggccaag gagccagcct ggtcgccgaa cgcgagccgc
     3301 atcgcaccgg tcacgatcat ggctccgaac gcgaggacga acagatacgt cggcacggcg
     3361 aagatcgtcc ccgattcgcg cagtccacgg agattcacga tcgttagcag cgcgacgaag
     3421 acgacggcca tctcgacgcg gtagtcgccg agatcgggga cggccgagac aactgccgct
     3481 acgccggcgg agatgctcac ggcaaccgtg agcacgtagt ccgtgagcag ggccgcggcg
     3541 gcgagcagag cggctcctat ccccagattc tcccgcgaca cggaatatgc gccgccaccc
     3601 tggggatagg cgcgaatcgc ttgacgatac gaggcgacga cggtcagcag caagaccacg
     3661 atggccagcg agatggggag cgaggggccg aggttgcctg agccggccag cacgaggacg
     3721 aggaggatct cctccgtcgc gtaagcagag gacgagagcg catcggacga gaatacggcg
     3781 agcgccttca ccttgctcag ccgctcctcc agagaccgcg atgaagcaag cggcggaccg
     3841 aacaggtatc gcctcgtcgc cgcccaggcg cggcccgcta ccgtctgagg ccgttcggca
     3901 gcgagcgtcg cttcccagtg tgtcgggctc gtgcgacgga acgggcgctc ctcgtcgagg
     3961 ctcacgcgaa cgtag
//
LOCUS       DATMBB010000004         4544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_35778, whole genome shotgun sequence.
ACCESSION   DATMBB010000004 DATMBB010000000
VERSION     DATMBB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35778"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VNL92_00070"
     CDS             complement(<1..225)
                     /locus_tag="VNL92_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWO93142.1"
                     /translation="MTDRAILEELRLLLERDALQQGDFTLSSGRRAAYYFDGRKVTLS
                     PRGAYLVGRAFVDLVRDSGAEAIGGLTLGAD"
     gene            complement(222..1055)
                     /gene="pheA"
                     /locus_tag="VNL92_00075"
     CDS             complement(222..1055)
                     /gene="pheA"
                     /locus_tag="VNL92_00075"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWO93143.1"
                     /translation="MPRLAFLGPPGTFGEIAALRLYPDDELVACTSHAAVAKAVAEGR
                     AERGVVAIENSLEGSVPETLDALISEAGLSICAETALPIRHALLGHKGARGEEVRVIY
                     SHPQALGQCRRFLERTYPDVPAEAALSTAAAIQEMLDHPGAAAIGNERAAALYGAAVL
                     QRDIQDDPSNTTRFVVLSTADAPPSGDDKTSLVFSTADRPGALVAVLRAFAERSINLT
                     KIESRPAKERLGVYVFLVDVEGHRLEGPVAEAIEDARRHTEYLRILGSYRRHHGSGNG
                     G"
     gene            complement(1061..1744)
                     /locus_tag="VNL92_00080"
     CDS             complement(1061..1744)
                     /locus_tag="VNL92_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWO93144.1"
                     /translation="MYIIVGGAGKVGYYLVRELMQLGHEVLTIERDRRRCELIAAEMG
                     DELVLHGDACEVAVLDHAGMGRADVYIAATGDDEDNLVSCQVAIHRFNVPRTIARINN
                     PKNEHIFRVLGIESTVSATAAVLSQIEQQLPQHPLIPLLSLRGSGLELVEVHVPDDAG
                     IVGRELRDVILPPRCLISLVVRTTGETEVATNETRIMAGDELVAVTPPENEDALREAL
                     TSITPVRAH"
     gene            complement(1747..2166)
                     /locus_tag="VNL92_00085"
     CDS             complement(1747..2166)
                     /locus_tag="VNL92_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HWO93145.1"
                     /translation="MRVLIMGCGRVGALLASMLDESGHEVTVLDMDEQAFRRLKPGFK
                     GTRMTGNGIDEDVLRRADIENVDAFLALTQGDNRNIMSAQIAKHIFRVPRVICRIYDP
                     QRDSIFRELGIETISPTTVGARILEQQLVGAAAQPEA"
     gene            complement(2166..2627)
                     /locus_tag="VNL92_00090"
     CDS             complement(2166..2627)
                     /locus_tag="VNL92_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO93146.1"
                     /translation="MVKLVANPTFADVKDVLVVVDGSQASVEAVELACRLARRRKGGV
                     YATYVIEVERSLPLDAELAQETTAGEEALRQAKATGKRYDHHVEVALLQARDAAQALV
                     SEAMERGVDAIIMGIDYERPYGEFQLQRIALQLLKHAPCQVWICRRRLEET"
     gene            complement(2743..3663)
                     /locus_tag="VNL92_00095"
     CDS             complement(2743..3663)
                     /locus_tag="VNL92_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO93147.1"
                     /translation="MSVLVVGGTGFIGREVMRRLARRGEKTIGFDINPRRSALADLGE
                     AVTIVAGDATKFDDLAAAVVDHKVDRIINLAYILGEGETMPHRAVQVDVVAMDNCFEI
                     TRLFGVKRCVFASSIAYHGPSQEPFGDRPVTEDDPGYPGGIYVDTKQFNERLAAGYNA
                     LYGTSIVALRPPFVMGPAKPRGVMDHVEIITRPALGLPVRSPSREDAPYIVGHVGDIA
                     EMFVRLTLAEAPHHTVYHAGGHTTTLGELAALVRQQIPAADIEFNPAGRGGFLVHRVD
                     NSRIVGEFEVAHRSLAQLVADIIEDTRRLT"
     gene            complement(3697..>4544)
                     /gene="glp"
                     /locus_tag="VNL92_00100"
     CDS             complement(3697..>4544)
                     /gene="glp"
                     /locus_tag="VNL92_00100"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWO93148.1"
                     /translation="PGANIRRRAEDVRAGDRILTEGTLLRPAEIGVLASLGRATVRVT
                     RRPVVAILSTGDEIVPPGEPLRPGTIYDSNASSIAALVRQFGGIPRVLGIARDTVEDL
                     TRRIHEGLDADMLVTSAGVSRGDYDVVKEVLAREGSVDFWTVAMKPGKPLAFGWFPEG
                     SRRVPHIGLPGNPVSSMVSFELFGRPAIAKMMGKPYWERPRLRAIALDGITNRNDPRT
                     FYARCRVDEEDGRWVARLTGPQGSGILTSMAFANALTIIPPEVDEVHPGDEIEVMMID
                     WSLGE"
BASE COUNT          756 a         1478 c         1519 g          791 t
ORIGIN      
        1 gtcggcgccg agggtcaggc cgccaatcgc ctcggctccg ctgtcgcgaa ccaggtcgac
       61 gaaggcgcgt ccgacgaggt aggcgccgcg cgggctcaac gtcaccttgc gcccgtcgaa
      121 gtagtaggcg gcccttcggc cggagctcaa cgtgaagtcg ccctgctgca gcgcgtcgcg
      181 ctccaggagc agccgcagct cctcgaggat ggcgcggtcc gtcatccgcc gttcccggag
      241 ccgtgatgac gccggtacga gccgaggata cggaggtact cggtgtggcg acgcgcgtct
      301 tcaatcgctt cggcgacggg cccctcgagt cgatgccctt cgacgtcgac gaggaacacg
      361 tacacgccga gccgctcctt ggccggccgc gactcgattt tcgtcaggtt gatggaccgc
      421 tcggcgaagg cccgcagcac cgcgacgagc gcaccgggcc ggtcggccgt ggagaacacg
      481 agcgacgtct tgtcgtcgcc gctcggcgga gcgtcggcag tggagagcac gacgaagcgc
      541 gtcgtgttcg acggatcgtc ttggatgtcg cgctggagga cggcggcgcc gtagagtgct
      601 gcagcgcgct cgttaccgat ggcggccgca ccggggtggt ccagcatctc ctggatggcg
      661 gcggcggtgg agagtgcagc ctctgccggc acatctggat acgtgcgctc gagaaatcgg
      721 cggcattggc ccagagcctg aggatgcgag tagatgaccc ggacttcctc cccccgcgcc
      781 cccttgtggc cgaggagggc gtggcggatg gggagggccg tctccgcgca aatcgaaagg
      841 cctgcttcgc tgatgagcgc gtccagcgtc tcgggtacgg aaccctcgag gctgttctcg
      901 atggcaacga ccccgcgctc cgctcgaccc tcggccacgg ccttggcgac agcggcatga
      961 ctggtgcatg cgacgagctc gtcgtcgggg tagaggcgta gcgccgcaat ctccccgaac
     1021 gtgccgggag gcccaagaaa cgcgaggcgc ggcatggcaa tcagtgagcc ctgaccggcg
     1081 tgatgctcgt gagagcctcc cggagagcgt cttcattctc agggggggtg acggcaacga
     1141 gctcgtcgcc ggccatgatc ctcgtctcgt tcgtggcgac ttccgtctct ccggtcgtgc
     1201 ggacgaccag cgagatgagg caccgcggcg gcaggatgac gtcgcgtagc tcgcgcccca
     1261 cgatgcccgc atcgtcggga acatgcacct ccacaagctc gagcccagat ccacggaggc
     1321 tgagcagcgg gataagcgga tgctgcggca gctgttgctc gatctgcgag aggacggcgg
     1381 cggtcgcgct gaccgtgctc tcgatgccga gcacccgaaa gatgtgctca ttcttagggt
     1441 tattgatccg agcgatggtg cgggggacat tgaagcgatg gatggcgacc tggcacgata
     1501 cgaggttgtc ctcgtcatcg cccgttgcgg cgatgtagac gtcagcgcgc cccatgccgg
     1561 cgtggtccag cacggcaacc tcgcacgcat cgccatgcag cacgagctcg tcccccatct
     1621 ctgccgcgat gagctcgcat cggcgacggt ctcgctcgat ggtgaggacc tcatgaccga
     1681 gctgcatcaa ctcgcgtacg aggtagtagc cgaccttccc cgcccctccg acgatgatgt
     1741 acacgcctac gcctccggct gtgcagcggc gccgaccaac tgctgttcga ggattctcgc
     1801 accgacggtg gtagggctta tggtctcgat gcccagctcg cggaagatgc tgtctcgttg
     1861 cggatcgtag atgcgacaaa tcacgcgtgg tacgcggaaa atgtgcttcg cgatttgggc
     1921 tgacatgatg ttacggttat ctccctgggt gagggcgagg aaggcatcca cgttctcgat
     1981 gtcagcccgt cgcagcacgt cttcgtcgat gccgttgccg gtcatgcgcg tccccttgaa
     2041 tcccggcttc agcctgcgaa atgcctgctc atccatatcg aggacggtga cctcgtggcc
     2101 tgactcatcg agcatggagg cgaggagggc gccgacgcgg ccgcagccca tgatgaggac
     2161 acgcatcagg tttcctccag ccttcggcgg cagatccaca cctggcaggg cgcgtgcttt
     2221 agcagctgga gagcgattcg ctgcaactgg aactcgccgt aaggtcgctc gtaatcgatt
     2281 cccatgatga tcgcatccac gccgcgctcc atcgcctccg agaccagggc ctgggccgcg
     2341 tctcgcgcct ggagcagggc cacctccacg tgatgatcgt accgctttcc ggtggctttc
     2401 gcttggcgga gagcttcctc tcccgcagtg gtctcctgag ccagctcagc atcgagtggc
     2461 agcgatcgtt cgacttcgat gacgtacgtg gcgtagacgc cacccttgcg tcgccgagca
     2521 agccggcagg ctaactcgac cgcttcgacg cttgcctgcg aaccatccac gacgacaagg
     2581 acatccttga cgtcggcgaa ggtcgggtta gccacgagtt tcaccacggc gtcgcttctc
     2641 tcgcacgggt cgcgatcctg cctgtctccc gaagcgcgtg aagtatagtg cgagcctaag
     2701 cctgccgcaa cccgagtgta caactaaacc tgatggcaga tgctaggtca ggcgtcgcgt
     2761 gtcctcgatg atgtcggcca ccagctgtgc gagcgagcga tgggccacct cgaactcgcc
     2821 gacgatgcgg ctgttatcga cccggtgcac gagaaagccg ccacgaccgg ccggattgaa
     2881 ctcgatatcg gccgcgggga tctgctgacg gacgagcgct gcgagctcgc cgagcgtcgt
     2941 cgtgtgtcct cccgcatggt agacggtgtg gtgcggcgct tcggcgagcg tgaggcgaac
     3001 gaacatctcc gcgatgtcgc ccacgtgtcc cacgatgtac ggcgcatcct cccgcgaggg
     3061 cgagcgcacc ggcaggccga gcgcgggccg cgtgatgatc tcgacgtgat ccatgacgcc
     3121 gcgcggcttc gccggtccca tgacgaaggg gggccggagc gcgacgatgc tcgtcccgta
     3181 cagggcgttg tagccggccg ccagtcgttc gttgaactgc ttcgtgtcga catagatgcc
     3241 accggggtag cctggatcgt cctcggtaac agggcggtcg ccgaacggct cctggctcgg
     3301 cccgtggtag gcgatcgagc tcgcgaagac gcatcgcttg actccgaaca accgcgtaat
     3361 ctcgaagcag ttgtccatgg ccacgacatc gacctggacg gcgcgatgcg gcatcgtctc
     3421 gccctcaccc aggatgtagg ccaggttgat gatgcgatcg accttgtggt cgacgacggc
     3481 cgcggcgagg tcgtcgaact tcgtagcgtc gcctgcgaca atcgtcaccg cttcgccgag
     3541 gtcggcgagg gcgctgcggc gtgggttgat gtcgaacccg atggtcttct cgccgcgacg
     3601 tgcgaggcgc cgcatcacct cccgaccgat gaacccggtt cctccgacga ctagtaccga
     3661 catcgcttcc ccctcggtca actatggtgc tgggcgttac tcgccgagcg accagtcgat
     3721 catcatgacc tcgatctcgt ccccggggtg gacctcgtcg acctcgggcg ggataatcgt
     3781 gagcgcgttc gcgaacgcca tcgaggtcag gatgcccgat ccctgcggcc cagtcagccg
     3841 cgccacccaa cgtccatctt cctcatcgac gcgacagcgg gcatagaacg tccgaggatc
     3901 gttccggttc gtgatgccgt ccagggcgat ggcgcgcaga cgcggtcgtt cccagtaggg
     3961 ctttcccatc atcttcgcga tggcgggcct gccgaacagc tcgaatgaaa ccatggagga
     4021 aacggggttt cctgggaggc caatgtgcgg cacgcggcgc gaaccctcgg ggaaccatcc
     4081 gaacgcgagg ggcttgccgg gcttcatcgc cacagtccag aagtccacgc tgccctcgcg
     4141 ggcgagcacc tccttgacga catcgtagtc gccgcgggac acgcctgccg aggtgacgag
     4201 catgtccgca tcgagaccct cgtggatgcg tctcgtcagg tcctccacgg tgtctcgggc
     4261 gatgccgagc acgcgcggga tgccgccgaa ctggcgcacg agcgcggcaa tgctcgatgc
     4321 gttgctgtcg tagatcgttc cgggcctcaa gggctcgccc ggcgggacga tctcgtcgcc
     4381 cgtcgacaga atggcgacga cgggacggcg cgtgacgcgc acggtcgccc gcccgaggga
     4441 tgcgaggacg ccgatctccg cgggacggag gagcgtgccc tcggtcagga tgcgatcgcc
     4501 ggcgcgaaca tcctcggcgc ggcggcgaat attggcgccg ggcc
//
LOCUS       DATMBB010000005         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_237484, whole genome shotgun sequence.
ACCESSION   DATMBB010000005 DATMBB010000000
VERSION     DATMBB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237484"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="VNL92_00105"
     CDS             <1..1974
                     /locus_tag="VNL92_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93149.1"
                     /translation="PGVGKTAIVEGLAQRIARGDVPEGLRGKRIVALDLGAMVAGAKY
                     RGEFEDRLKAVLKEVQEAEGQILLFIDELHTVVGAGAAEGAMDASNMLKPMLARGELH
                     CIGATTLDEYRKHIEKDAALERRFQPVRVGEPTLEDTISILRGLRERYEVHHGVRIKD
                     GALVAAAVLSNRYITDRFLPDKAIDLVDEAAAKLRTEIDSLPAELDEVERRILRLEVE
                     REALRKESDPASRERLERLERELASAREEQSALRAQWQAEKERIQAQRRLREEIERTN
                     IEIELAERRADYETAARLRYSTLSELEAKLKQLEAEDQSAHRLLKEEVGEDDIAEVVA
                     RWTGIPVSRLVESEMYKLIHMEERLHERVVGQDDAVRAVANAIRRSRAGLQDPNRPVG
                     SFIFLGPTGVGKTELARALAEFLFDDERAVIRIDMSEYMEKHSVSRLIGAPPGYVGYD
                     EGGQLTEAVRRRPYSVVLFDEIEKAHNDVFNVLLQVLDDGRLTDGQGRVVDFKNTIIV
                     MTSNLGSAFIAQMAGDENAMREMVMETVRTHFRPEFLNRIDEIVIFKQLSQADIAAIV
                     EIQLRLVAKRLADRQLTLDVTPAAREALATMGYDPVYGARPLKRLIQRSVLDPLALRL
                     LAGEFSEGSTIVVDWNGEAFEFRAVVEGEVMAA"
BASE COUNT          383 a          667 c          718 g          330 t
ORIGIN      
        1 ccgggtgtcg ggaagacggc catcgtcgag ggactggcgc agcgcatcgc tcgcggcgac
       61 gtgcccgaag ggctccgcgg caagcgcatc gtcgcgctcg accttggcgc gatggtggcc
      121 ggcgcgaagt accgcggcga gttcgaagac cgcctgaagg ccgtgctcaa ggaggtgcag
      181 gaggccgagg gccagatcct gctcttcatc gacgagctcc acacggtggt cggggcgggc
      241 gctgccgagg gggccatgga tgcctccaac atgctgaagc ccatgctcgc ccgtggcgaa
      301 ctgcattgca tcggcgccac cacgctcgac gagtaccgca agcacatcga aaaagatgcg
      361 gcactcgaac gccgcttcca gcctgtccgc gtgggcgagc ccacgctgga agacaccatc
      421 tccatcctcc gtggcctccg cgaacgctat gaggtccacc atggcgtgcg gatcaaagac
      481 ggtgcgctcg tcgccgcggc cgtgctgtcc aaccggtaca tcactgaccg tttcctgccc
      541 gacaaggcca tcgacctcgt cgacgaggcg gcggcgaagc tgcgtacgga gatcgattcc
      601 ctccctgccg agctcgacga agtggagcgc cgcatcctgc gcctggaggt ggaacgcgag
      661 gcgttgcgca aggaatccga tcccgcttcc cgcgagcgcc tcgagcggct ggagcgcgaa
      721 ctcgcctccg cgcgcgagga acagtcggcc ctgcgggccc agtggcaggc agaaaaggag
      781 cgcatccagg cgcagcgtcg gctccgcgaa gaaattgaac gcacgaacat cgagatcgag
      841 ctcgccgagc gccgcgcgga ctatgaaacc gcggcgcggc tgcgctattc gacgctttcc
      901 gagctggaag cgaagctcaa gcagctcgag gccgaggacc aatcggcaca ccgtctcttg
      961 aaggaagaag tcggcgagga cgacatcgcc gaggtggttg ctcgctggac gggcatcccc
     1021 gtcagccgcc ttgtcgagag cgagatgtac aagctgatcc acatggaaga acggctgcac
     1081 gagcgcgtcg tcgggcagga cgatgccgtc cgtgcggtgg cgaacgccat ccggcgctca
     1141 cgggcgggcc tgcaggaccc gaaccggccg gtgggttcgt tcatcttcct gggcccgacc
     1201 ggcgtcggga agacggaatt ggcacgagcc cttgccgagt tcctcttcga cgatgagcgg
     1261 gccgtcattc gcatcgatat gagcgagtac atggagaagc attccgtctc gcgactcatc
     1321 ggcgccccgc cgggatatgt tggctatgac gagggcgggc agctgaccga ggccgtgcgg
     1381 cggcggccct attcggtcgt gcttttcgac gagatcgaaa aggcccacaa cgatgtgttc
     1441 aacgtgctgc tgcaggtgct cgatgatgga cgtctgacgg atggccaggg ccgcgtcgtg
     1501 gacttcaaaa acacgatcat cgtgatgacg agcaacctcg ggagcgcctt catcgcccag
     1561 atggcaggcg acgagaacgc aatgcgcgag atggtgatgg agacggtgcg gacacacttc
     1621 cggccggagt tcctgaaccg catcgacgag atcgtgatct tcaagcagct ctcgcaggcc
     1681 gacatcgccg cgatcgtcga gattcagctg cggctggtgg cgaagcgact cgccgaccgc
     1741 cagctcacgc tcgatgtcac gcctgcggcg cgggaggcgc ttgccacgat ggggtatgac
     1801 ccggtctatg gcgcgcggcc tctcaagcgc ctgatccagc ggtcggttct cgacccactg
     1861 gcgctccggc tccttgccgg agagttcagc gaagggtcga cgatcgttgt cgattggaac
     1921 ggggaggcgt tcgagttccg cgccgtggtc gaaggcgaag tgatggccgc ctgaggcgat
     1981 cccctcgctg cccggttcag cggtccgcgc gcacgcgccg gacgatcgac atgacactcg
     2041 ccgtgtccgc ggcgagcgtc gcccagtcgc ggtcgccgag aattcggctg gagatcag
//
LOCUS       DATMBB010000006         5220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_508545, whole genome shotgun sequence.
ACCESSION   DATMBB010000006 DATMBB010000000
VERSION     DATMBB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508545"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VNL92_00110"
     CDS             <1..561
                     /locus_tag="VNL92_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWO93150.1"
                     /translation="VEAIEAISASSSRLVRTLDTFLDIARLGSASLHVEQAPFDLVET
                     IEEELTAVRMRYPEAIVSTSCPPEPIEVCSDELRVRQVLVNLLDNAAKYGGEPAIIDV
                     VAERNDHGASIRVSDNGPGVAPEDQAHLFERFYRGSRQPKKGLGVGLFLSRQIVQQLG
                     GTLTFRNLPGGGAEFTLLLPIVKKRT"
     gene            complement(558..1028)
                     /locus_tag="VNL92_00115"
     CDS             complement(558..1028)
                     /locus_tag="VNL92_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWO93151.1"
                     /translation="MVSADTPLVQAARLRSLVWRSGAPRRAHADDRDIAISAGHVLVI
                     DDDPDMRRLLRFALNDFGFQVTTAENGAEALAQLEEGAHPHAIVLDLEMPRMDGRAFY
                     AELRRRGIDTPVLVLSAYGAERAARELKLRFALAKPFDLEELVQTLRNMIGGSG"
     gene            1176..1910
                     /locus_tag="VNL92_00120"
     CDS             1176..1910
                     /locus_tag="VNL92_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWO93152.1"
                     /translation="MTQEQHAVKALILAVDDEPGILKLINRLLLEQEYRVVSAKTGEE
                     ALQMAEEYRPDLVILDLMLPDRDGLEIMRHLRERMDVPIIILTARGSDHDKVAGLELG
                     ADDYLAKPFNPEELTARVNAVLRRTHTGASGGTRVVIGDVEIDLAKRAVYKRGELIRL
                     TRTEWLLLQTLAANAGKVVLHTELLSKVWGPEYRDDLQYLRVWISRLRRKLEDIPSEP
                     QLIKTFQGIGYLLDSEAALTEAARGQ"
     gene            2049..2843
                     /locus_tag="VNL92_00125"
     CDS             2049..2843
                     /locus_tag="VNL92_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO93153.1"
                     /translation="MDLGLNGRVAIVTGGGGPGNGGHIARTLAREGATVVIVDLSKEN
                     GEARASEIREAGGTALPLVANAASWPDVQGVVAEALRSFGRIDVLVNSAAGANNKLFI
                     ESSPEDWPRDIDGCLVAALNCSRAVLEPMRDAGWGRIVNIISDAGRVGEVRQSVYSGA
                     KAGIIGFSRALAKEVGRYGITVNNVSPAVTEREDATLGAWYWGATPEEQQRRRERAMR
                     AYPLAAAYHRFGRPQDVANAVAFFCSEAAMWVTGQTLSVNGGYSMI"
     gene            2890..3654
                     /locus_tag="VNL92_00130"
     CDS             2890..3654
                     /locus_tag="VNL92_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HWO93154.1"
                     /translation="MAINKVVPTFDEAVADAQDGSVIHIGGFGGPAECPSYLIAALAR
                     KQIRDLTITGNLAGWGVQMISLLRERMAMALPFPEDFFDPGLLVEQGCVRKGILAFPA
                     APGRVELPFERALREGKVEVELLGQGTLAERIRAARAGIAAFYTPVGPGTIVAEGKEV
                     RDFDGIPHLLERALPADFSIIRAHKADPRGNLVYRGTGRTLNATMAGAARVTIAEVDE
                     VVALGDLDPECIATSGVYVQRIVVRPATPKRWDEAC"
     gene            3678..4349
                     /locus_tag="VNL92_00135"
     CDS             3678..4349
                     /locus_tag="VNL92_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HWO93155.1"
                     /translation="MPGEKLDEVTMAMRAAREFEDGMLVNLGVGIPTLASSYVLPGRE
                     VIFHSENGVIGFGPLVEDPSQADPYLINASVQPVSPLPGMCFVSHEEAFAMIRGGHVD
                     LTVLGALQVAENGDLANYHLPGKVTGSFGGGQDLAFCAKRVVIVMTHQTKEGEPKVVS
                     RVTLPLTAPRAVDRIITDVAVMDVTSDGLVLRECVPGWTPDDIQAITEATLHVPDDVT
                     EMTLA"
     gene            4460..>5220
                     /locus_tag="VNL92_00140"
     CDS             4460..>5220
                     /locus_tag="VNL92_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWO93156.1"
                     /translation="MMAELNGGQLIAKQLAQAGIDTVFGVVAGPMLQAFAALPGEGIR
                     VVGCRHEEQAAFMAQAWGYITKKPGVVIAGSGPGMTNTITSLYVAQANCWPLVVLGGS
                     APGATRGLGGFQEADQVAYAAPACKWATQVDSAERVPEYVHLALGRALAGRPGSVYLD
                     FPAQVMAAGVPEDRVRLRTTEPRMYRPNADAAGIADVADMLLQAERPLVLIGKGAAWA
                     DAAEPLQRLVNLGLPFAASPMGRGTVPDDSPMCVGG"
BASE COUNT          957 a         1708 c         1678 g          877 t
ORIGIN      
        1 gtggaagcga tcgaagccat ctcagcttca tcgagccgct tggtccgaac gctcgatacg
       61 tttctcgaca tcgcacggct cggatcggct agcctccatg ttgaacaggc acccttcgat
      121 ctcgtcgaga ccatcgagga agagctaacg gccgtgcgca tgcggtatcc cgaggcgata
      181 gttagcacat cgtgcccgcc cgaaccgatc gaggtctgct ccgatgagtt gcgcgtgcga
      241 caggtgcttg tgaatctgct ggataacgcc gcgaaatacg gaggcgagcc agccatcatc
      301 gatgttgtgg ccgagcgcaa cgatcacggc gcctcgattc gcgtcagcga caacggtccc
      361 ggtgtggctc cagaggatca ggcgcatctc ttcgagcgct tctatcgggg ctcgcggcag
      421 ccgaaaaagg gccttggcgt cggcttgttc ctctcccgac agattgtgca gcagcttggc
      481 ggaacgctca cgttccgcaa cctgcccgga ggcggagccg agtttacgct gctgctgccg
      541 atcgtgaaga agcgaaccta accagaacca ccgatcatgt tccgaagcgt ttgcacgagc
      601 tcctcaagat cgaacggctt cgccagggca aaccgcaact tcaattcgcg agcggcgcgc
      661 tcagcgccat aggcggagag cacgagaaca ggagtatcga tgccgcgcct gcgcagctcc
      721 gcatagaacg cgcggccgtc cattctcggc atctccagat cgagcacgat cgcgtgcgga
      781 tgcgcaccct cctcgagctg ggcgagggct tccgccccat tctccgccgt cgttacctga
      841 aacccaaagt cgttgagtgc gaaccgcaga aggcggcgca tatctggatc gtcatcgata
      901 acgagaacgt gcccggcgga tatggcgata tcgcgatcgt ctgcgtgcgc gcggcgcggc
      961 gcgccgctcc gccagaccaa ggagcgaaga cgggctgcct gtactaacgg agtatcagcg
     1021 ctaaccatat actgccgagc ataccattcc ctactccagt agacacgagc gcctggttcg
     1081 cgtacagtcg gtggcagaca caacatttcg tctagttgcg gccatcagac gttatgttcc
     1141 tgggtccatt acatcgagga ttgacatcaa gcatcgtgac gcaagaacag catgctgtca
     1201 aagctctaat cctggcagtg gacgacgagc cgggcatact caagctcatc aaccgactcc
     1261 tccttgagca agagtatcgt gtcgtatcgg ccaaaacggg cgaagaggcc ctccagatgg
     1321 cggaggagta tcgccccgac ctcgtcatcc tcgatctcat gctacccgat cgcgatggtc
     1381 ttgagataat gaggcacctc cgggaacgca tggacgtgcc gatcattatc ctcacggcga
     1441 gggggagcga ccacgacaag gtcgccggcc tggaattggg agccgatgac taccttgcca
     1501 agccattcaa tcccgaggag ctgacggcgc gcgtcaatgc cgtgcttcgg cggacgcata
     1561 cgggcgcctc gggcggcacg cgggtcgtga taggcgacgt cgagatcgat ctggcaaagc
     1621 gcgccgtgta caagcgcggc gagctcatca ggctgacccg aacggaatgg cttcttctgc
     1681 aaacactagc agcgaacgcc ggcaaggtcg tgcttcatac ggagctcctg agcaaggtct
     1741 ggggcccgga gtatcgcgac gacctccagt atctccgcgt ttggatatcg cgcctgcgcc
     1801 ggaagctcga ggacatccca agcgagcctc aattgatcaa gacgttccag ggcatcggct
     1861 atctcctcga ttccgaagcc gcactcacgg aggccgcgcg gggccaatag ctcccggtca
     1921 ccggtgccgc atcgccgcgc cgtccccctc ggccgccgtg cccgttgccg atgcgccgcg
     1981 tcacggttat gatgccgccc gtgctgtgag gagatggcgg acgccatgac ggcgccggag
     2041 gagcgaccat ggatctcgga ctcaatggac gcgtcgcgat cgtgaccggc ggcggtggac
     2101 cagggaacgg tggccacatc gcccgcacgt tggcgcgcga aggcgcaacg gtcgtcatcg
     2161 tcgacctgag caaggagaat ggtgaggcgc gcgcatccga aatccgggag gccggcggca
     2221 cggcgctccc gctcgtcgcc aatgcagcct cctggcccga tgtgcagggc gtcgtggcgg
     2281 aagcgcttcg ctcgttcggc cgcatcgacg tcctcgtcaa tagcgcggcg ggggccaaca
     2341 acaagctctt catcgaaagc agcccggagg attggccacg cgacatcgac ggatgtctcg
     2401 tcgcggcgct caactgctcc cgagctgtgc tcgagcccat gcgcgatgcg ggctgggggc
     2461 ggatcgtcaa catcatttct gatgcaggac gcgtgggcga ggtgaggcag agcgtctact
     2521 cgggcgcgaa ggccggcatc atcggattca gccgcgcgct cgcgaaggaa gtgggacgtt
     2581 acggcatcac cgtgaacaac gtctcccctg ccgtcacgga gcgcgaagat gccacgctgg
     2641 gcgcgtggta ttggggagcg acgcccgagg agcagcagcg ccgccgcgag cgggccatgc
     2701 gggcctatcc gctcgcggca gcgtaccatc gcttcggccg gcctcaggac gtcgccaacg
     2761 ctgtcgcctt cttctgttcg gaggccgcga tgtgggtgac gggacagacc ctgagcgtca
     2821 acgggggcta ctcgatgatc tagacgcttg ctcgcacccc gtggaaagat acttcacata
     2881 ggcggaaaga tggcgatcaa caaagttgtt ccaacgttcg acgaggccgt cgctgacgcg
     2941 caggacggct cggtcatcca tatcgggggc tttggcggcc cggccgagtg cccgagctac
     3001 ctcatcgccg cgctcgcccg aaagcagatc cgcgatctga ccatcacagg caacctcgct
     3061 ggctggggcg tccagatgat ctcgctgctc cgagagcgca tggctatggc gcttcccttc
     3121 cccgaggact tcttcgaccc aggcctgctc gtcgagcaag gatgtgtacg caaaggcatc
     3181 ctcgccttcc ccgctgcccc cggccgcgtc gagttgccgt tcgaacgggc cttacgcgaa
     3241 ggcaaagtgg aggtcgagct gctcggtcag gggacactcg ccgaacgcat ccgagctgcg
     3301 cgagccggaa tcgccgcctt ctacacgccg gttggaccgg gcacgatcgt tgcggagggc
     3361 aaggaagtcc gtgacttcga tggcatcccg catctgctcg aacgcgccct cccggccgat
     3421 ttctcgatta tccgcgccca caaggccgac ccgaggggca acctggtcta ccgtggcacc
     3481 gggcgcacac tcaatgcgac catggcgggg gccgcgcgcg tcaccatcgc cgaggtcgac
     3541 gaggtcgtgg ccctaggcga cctcgacccg gagtgcatcg cgacctcggg cgtgtacgtg
     3601 cagcgcatcg tcgtccgtcc cgcgacaccc aagcgctggg acgaggcgtg ctgacagcac
     3661 cagagggagg agcacgtatg ccaggagaga agctggatga ggtgacgatg gcgatgcgag
     3721 cggctcgcga gttcgaggat ggcatgctcg tcaacctcgg cgtcggcata ccgaccctcg
     3781 ccagcagtta cgtcttgccc ggccgcgagg tcatcttcca ttccgagaac ggcgtcatcg
     3841 gcttcggccc gctggtcgag gacccttccc aggccgaccc gtatctgatc aatgccagcg
     3901 ttcagcccgt ctcgccgctg cccggcatgt gcttcgtcag ccacgaagaa gccttcgcga
     3961 tgattcgcgg tggccacgtc gatctcactg tgctcggggc tctccaagtg gcggaaaatg
     4021 gcgatctggc gaactatcac ctgcccggca aggtcacggg aagcttcggc ggggggcagg
     4081 accttgcctt ttgcgcaaag cgcgtcgtca tcgtcatgac ccaccaaacc aaggagggag
     4141 agcccaaggt tgtctcccgc gtgacgctgc cgctcaccgc gccgcgcgcc gtcgaccgca
     4201 tcatcaccga cgtcgccgtc atggatgtca cgtccgatgg cctcgtcctc cgcgagtgcg
     4261 tgccgggctg gactccggac gatatccagg ccatcaccga ggccacgctt cacgttccgg
     4321 acgatgtcac ggagatgacc ctcgcgtagg agcaccggcc accccccgcc cccgtccgtg
     4381 tcgatagagt ggacggcagc gaagcccgcc tgctacagtt cgccctggtc caatatggag
     4441 ctcttgcgcg aggggggaga tgatggctga gctcaacggc ggacaattga tcgcaaagca
     4501 gctggcgcag gcaggcatcg acacggtctt cggcgtcgtc gctgggccga tgctgcaggc
     4561 attcgcggct ctgcccggcg agggcatacg cgtcgtgggc tgccgccacg aggagcaggc
     4621 ggccttcatg gcccaggcct ggggttacat caccaagaag ccaggcgtcg tcatcgccgg
     4681 gtccggcccg gggatgacga acaccatcac gagcctctac gtcgcccagg ccaactgctg
     4741 gcccctcgtc gtgctcggcg gctcggcgcc aggcgctact cgcggcctcg gcggattcca
     4801 ggaggccgac caggtggcct acgcggctcc cgcatgcaag tgggccacgc aggtcgatag
     4861 cgcggagcgc gtgccggaat acgtacactt ggcgctcggg cgagcgctcg cgggacggcc
     4921 cggatccgtc tacctcgact tccccgcgca ggtaatggcc gccggcgtgc cggaggatcg
     4981 tgtccgcctc cgcacaaccg agccccggat gtaccgtccg aatgccgatg ccgccggcat
     5041 cgcggatgtt gcagacatgc ttctccaggc ggagcggccc ctcgtcctca tcggcaaggg
     5101 agctgcctgg gcggacgcgg ccgagccgct gcagcgcctc gtaaaccttg gcctgccatt
     5161 cgccgcctcg ccgatgggac gaggcacggt tcccgacgac agcccgatgt gcgtcggcgg
//
LOCUS       DATMBB010000007         3119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_33388, whole genome shotgun sequence.
ACCESSION   DATMBB010000007 DATMBB010000000
VERSION     DATMBB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33388"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..572)
                     /locus_tag="VNL92_00145"
     CDS             complement(21..572)
                     /locus_tag="VNL92_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWO93157.1"
                     /translation="MIRALGDKRPRIHPAAFVSEAAYVIGDAEIGEGATIWPGVVIRA
                     DFNRIVIGAGTHVEDNSVLHAVPAPLVIGQGCTIGHGVVVHAERIGDHCMIANGSTVL
                     DGVVVGDWSMIGAGSLVTPRMQIPPRSLVFGNPAEVQGEIRPHHEERLRRAGEVHIEM
                     SRRYVELGLAADISEFAIAAEAR"
     gene            complement(569..1351)
                     /locus_tag="VNL92_00150"
     CDS             complement(569..1351)
                     /locus_tag="VNL92_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO93158.1"
                     /translation="MQLQGKVALVTGASRGLGRIMALALADDGADVICAARTTEDSPS
                     KLPGTIDRTAAEVRERGRRSLALPMNLVDEVQVSDAVERALGEFGRVDILVNNAGVSF
                     RGSVLEQGLARWDLVLGVNLRGAVQLCMLLAPGMVERGEGRIINISSGYASTPGTGRV
                     SYATSKAALNKFTEGFAGEMRGKGVAVNCIELELGLATEGFRFVNPDVDTSSWPDPEI
                     MGDAAVWLARQPLDYTGNVLRIADIRTALERVHRGEPAREAG"
     gene            complement(1442..2656)
                     /locus_tag="VNL92_00155"
     CDS             complement(1442..2656)
                     /locus_tag="VNL92_00155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93159.1"
                     /translation="MGWRRTFATLEIPEYRTLWWGMIASFVAMQMNIVARGYLAYDIS
                     GSAAALGLVSLAWGLPMLAFSLVGGAVADRMRKRDILIQSQVAMGVLALINAVLVHLG
                     VIEIWHLVVLGLGQGTVFAFNMPARSAIIPEIVGHDRLQNAFALNNAGMNMSRIVGPA
                     VAGGLISIPFFGMTGTFYLMAALYVVVLGSLLLLPKGKAPERVKAPMLEDIAAGFIHI
                     RRNEVLLMLMSMAFVPVLFGMPYQTLMPVFQKDVLHVGSLGLGLLLGATGVGALVGSL
                     FVASLEDGRMRGQIQLVMGLSFGVSLVIFAAMQSFVLALIAIGLVGLVSSAYMSLNQT
                     MIMSYTEEDYYGRVMSVYMMTFSLMPLSAMPMGLLADSVGAPLTVAGAAALIVLFMAG
                     LAFLVPGYRRIQ"
     gene            complement(2901..>3119)
                     /locus_tag="VNL92_00160"
     CDS             complement(2901..>3119)
                     /locus_tag="VNL92_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWO93160.1"
                     /translation="AEYVARHMTKPVVAFIAGSTAPPGRRMGHAGAIIAGDAGTAAAK
                     KDALRTAGAVICDSPAEIGVTMASVLKR"
BASE COUNT          567 a         1014 c         1024 g          514 t
ORIGIN      
        1 tgtggtttcc ccgcagttgg ttaccttgct tccgccgcga tcgcgaattc cgagatgtcg
       61 gctgcgaggc ccagctccac gtaacggcgc gacatctcga tgtgaacctc gcccgcacgc
      121 cgcaggcgct cctcgtggtg aggccgtatc tccccctgca cctcggccgg attgccgaaa
      181 acaagcgaac gcggcgggat ctgcatcctg ggcgtcacga gcgaaccagc gccgatcatg
      241 gaccagtcgc cgacgacgac gccatcgagg actgtggagc cgttcgcgat catgcaatgg
      301 tcgccgatgc gctcggcgtg cacgacgacg ccatgaccga tagtgcagcc ctgcccgatg
      361 acgagaggtg ctgggactgc atgcaggacg gagttgtctt cgacatgcgt gccggctccg
      421 atgacgatgc ggttgaagtc ggcgcgaatc accacgccgg gccagatcgt cgccccctcg
      481 ccgatctcgg catcgccgat gacgtaggcg gcctcgctca cgaaggccgc cgggtgaatg
      541 cgcggcctct tgtcgccaag ggctcgtatc atcccgcctc cctcgccggc tcgcctcgat
      601 gcacgcgttc cagcgcggtc cggatatcag cgatgcggag cacgttgccc gtatagtcga
      661 ggggctgtcg cgcgagccag acggcggcgt cgcccatgat ctcgggatcc ggccagctgc
      721 tcgtgtcgac atcggggttc acgaaccgga agccttccgt cgccaagccc agttccagct
      781 cgatgcagtt cacagcgaca cccttgccac gcatctcgcc ggcgaagccc tctgtgaact
      841 tgttcagagc tgccttcgac gtagcgtagg agacgcggcc cgttccgggc gtgctcgcat
      901 accccgacga gatgttgatg atccgtcctt cgccgcgctc caccatgccg ggcgcgagga
      961 gcatgcagag ctgcacggct ccgcgcaggt tgaccccgag caccaggtcc catcgcgcca
     1021 agccctgctc gagcaccgac ccgcggaagc tcacgccggc gttgttcacc aggatgtcga
     1081 ctcggccgaa ctcaccgagc gcgcgctcga cggcgtcgct gacctgcacc tcgtcgacca
     1141 ggttcatggg caaggcgagg ctgcggcggc ctcgctcacg cacttcggcc gccgtgcgat
     1201 cgatcgtgcc gggcagcttc gagggcgagt cctcggttgt gcgggccgcg cagatgacat
     1261 cggcaccgtc gtcggcaagg gcgagggcca tgatgcgacc aagaccgcgg cttgctccgg
     1321 tgacgagcgc gactttgcct tgaagctgca cagtgcctct tccgaatgtt aggtaggcgc
     1381 gtgggcgcgc ggagtatacc acacgagggc cagcatgtgg cgcttcgggg cgctgcggtc
     1441 cctattggat gcgacggtat cccggaacga ggaacgcaag ccccgccatg aacaggacga
     1501 tgagcgccgc ggccccggcc accgtcaggg gagcgccgac gctatcagcg agcaagccca
     1561 tcggcatggc ggaaagcggc atgagcgaga acgtcatcat gtagacgctc atcacccggc
     1621 cgtaatagtc ctcctcggtg tagctcatga tcatcgtctg attcagcgac atgtacgcgg
     1681 aactcacgag accgacgaga cctatcgcga tgagcgccag gacgaacgac tgcatcgctg
     1741 cgaagattac gagactgacg ccgaagctca atcccatcac gagctggatc tggccccgca
     1801 tgcgtccatc ttcgagggag gcgacgaaga gcgagcccac gagggcgccc acgcccgttg
     1861 cgcccagaag aaggcccagg ccgagcgatc cgacgtggag cacgtccttc tggaacacgg
     1921 gcatcagcgt ttgatacggc atgccgaaca acaccggtac aaaggccatg ctcatcagca
     1981 tgagcagcac ctcgttccgg cggatgtgga tgaatcccgc ggcgatgtcc tcgagcatcg
     2041 gcgccttgac gcgctccggc gctttcccct tggggagcaa caggagcgac ccgaggacta
     2101 cgacgtagag ggccgccatc aggtagaacg tgcccgtcat gccgaagaac gggatgctga
     2161 tgagaccgcc ggctaccgcc ggaccgacga tgcgactcat gttcatgccg gcgttgttga
     2221 gggcgaatgc gttctgcagg cggtcgtggc ccacgatctc ggggatgatg gccgaccgtg
     2281 ccggcatgtt gaatgcaaac acggtgcctt ggccgagtcc gaggacgacg aggtgccata
     2341 tctcgatgac gccgaggtgc acaagaacag cattgatgag ggcgagtaca cccatcgcca
     2401 cctgcgactg gatgaggatg tcgcgcttgc gcattcgatc ggccacggcg ccgcccacga
     2461 gggagaacgc aagcatcggc aagccccaag cgaggctcac caatccgagc gcggcagccg
     2521 aacccgagat gtcgtaggcg aggtatcccc gcgcgacgat gttcatctgc atcgccacga
     2581 aggaggcgat catcccccac cagagggttc ggtattcggg gatctccaac gtcgcgaagg
     2641 tgcgccgcca ccccactcgg gacgcggagg cggagcgcga tgcttcggta gcgtcgcttc
     2701 gggttgctgg aatgaccctc gggttgcttg ccaggggact gtcctaggat gctactgcgc
     2761 cggcgccggc cgggcgcgat cagacttgtc tttcgagcat accgtttacg tgcgcgtaag
     2821 ggggcactgg tccgtgaact cgtaccttag gcgggaggca gatcatgtcg gacgagcgaa
     2881 gtaagccatg ggaccggacg tcagcgtttg aggacgctcg ccatcgttac gccgatttca
     2941 gcggggctgt cgcagatgac ggcgcccgcg gtgcgcagcg cgtccttctt cgctgccgcg
     3001 gttccggcgt cgccggcaat gatggcgcca gcatggccca tccgccggcc gggcggcgcg
     3061 gtgctgccgg cgatgaacgc gacgaccggc ttggtcatgt ggcgagcgac gtactccgc
//
LOCUS       DATMBB010000008         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_32926, whole genome shotgun sequence.
ACCESSION   DATMBB010000008 DATMBB010000000
VERSION     DATMBB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32926"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1620)
                     /gene="secD"
                     /locus_tag="VNL92_00165"
     CDS             complement(175..1620)
                     /gene="secD"
                     /locus_tag="VNL92_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWO93161.1"
                     /translation="MRRRTNQIALAFIFIITALGLYIVWPDEPDRYFPDLIPWPSGRG
                     VEIGDFEREGMRLGLDLQGGTRIVLEADPTYEGNLDDALEGARRVIEDRVNEFGVSES
                     EINRLGENRLEVQLPGVDPEEARDLVGRTATLDFRIPQESPGGGAATGPDGGPVWIPA
                     TGVINGETVALTGEFLEANSFVTSDALGRPAVAIEFNGTGAELFEQITNSLAQRGSDD
                     LRLLGIFLDDELISAPLVDERIPGGQAQITGVDRDEGKRLSVQLNAGALPVSLNVVQQ
                     NDVDATLGEETVLDTVRAGELALIAVAVFMIVYYRLPGLLATGALFTYTVVLLSIFKL
                     WPVTITLSGIAAFVLSVGMAVDANILIFERMKEELRAGRSLQASIDTGFSRAWSSIRD
                     SNISTLITAGILYWFGDQFAESLVKGFAITLAIGVLVSMITAIVVTRTFLHAVIGTPL
                     ARRLGLFASDIPERPQGGRPVPRGAKAEEVS"
     gene            complement(1641..1955)
                     /gene="yajC"
                     /locus_tag="VNL92_00170"
     CDS             complement(1641..1955)
                     /gene="yajC"
                     /locus_tag="VNL92_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWO93162.1"
                     /translation="MELMFFLIALFVAFYFILLRPVLKQQKQQRREMSDLRVGDEVLT
                     SAGIFATVAAIDVPETGPIRLTLELAPGVRVQAVPQAILQRVTENGESEAPQLSNETA
                     RE"
     gene            complement(1968..>2287)
                     /locus_tag="VNL92_00175"
     CDS             complement(1968..>2287)
                     /locus_tag="VNL92_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase"
                     /protein_id="HWO93163.1"
                     /translation="DTSIAILVLSLADHMAARGPRIEGRDFMSHVAYIAYVVDRWRWN
                     RVGITRHRLLTGHDIMEEFRLPPGPLVGRVLRAVDEAQGLGLARTRDEAVHVAREALA
                     TCR"
BASE COUNT          429 a          759 c          728 g          371 t
ORIGIN      
        1 gtcgcagttc ctcggacccg acaggatcct ggaaggcaac ggtgaactgt gtgcccgccg
       61 tgaagtcgat gcccggcttc agcgaaggtg ggatgagcag cgagacaacc cccggaacga
      121 gtatcagcac cgaaaacaag aagaaccagc cgcgcttccc ggcgaagtcg aacatcagct
      181 cacctcctcc gccttcgcgc ctcgaggcac cggccgcccg ccctggggcc gctctggtat
      241 gtcggaggca aagagcccta gccgccgggc gagcggcgtg ccgatgaccg cgtgcaggaa
      301 cgtccgcgtc acgacgatgg ccgtgatcat gctaaccagc acgccgatgg cgagcgtgat
      361 ggcgaagccc ttcacgaggc tttccgcgaa ctgatcgccg aaccaataca ggattccggc
      421 cgtgattagc gtcgagatat tgctgtcgcg gatcgacgac caggcgcgcg aaaagccggt
      481 gtcgatcgag gcctggaggc tccgcccagc ccgaagctcc tccttcatcc gctcgaagat
      541 gaggatgttc gcgtcgacag ccatgcccac ggacagcacg aatgccgcga tgcctgacaa
      601 cgttatcgtc acgggccaca gcttgaagat ggacagcagc accacggtgt aggtgaagag
      661 ggcacccgtc gcgaggaggc cgggcagccg gtaatacacg atcatgaaga cggccacggc
      721 gatcaatgcg agctcgccgg cgcggaccgt gtcgagcacc gtctcctcgc cgagcgtggc
      781 gtcgacgtcg ttctgctgca cgacattcag cgagacggga agggcgcctg cgttcagctg
      841 cacggagagc cgcttgcctt cgtcgcggtc cactcccgtg atctgcgctt ggccacccgg
      901 aatgcgctcg tcgacaagcg gtgcgctgat gagctcgtcg tcgaggaaga tgccaagcag
      961 gcgaagatcg tcgctgccgc gttgcgctag cgaattcgtg atctgctcga agagctcggc
     1021 tcccgtgccg ttgaactcga tggctacggc cggccggccg agcgcgtcgc tcgtcacgaa
     1081 ggaattcgcc tcgaggaact cgccggtgag ggcgaccgtc tcgccgttga tgacgcccgt
     1141 cgccgggatc cacacgggac caccatccgg gcccgtcgcc gcgcctccgc cgggcgattc
     1201 ttgagggatg cgaaagtcga gggttgcggt gcggcccacg aggtcgcgcg cctcctccgg
     1261 gtcgaccccc ggcagctgaa cctcgagccg attctcgccg aggcggttga tctcggactc
     1321 ggacacgccg aactcgttca cgcgatcctc gatgacgcgc cgggccccct ccagcgcgtc
     1381 gtcgaggttg ccctcgtagg tcgggtcggc ctcgagcacg atgcgcgtcc caccctgcaa
     1441 gtcgaggccc agccgcatcc cctcgcgctc gaaatcgcct atctcgactc cgcgaccact
     1501 gggccacggg atgagatccg gaaagtaccg gtcgggctca tcgggccaaa cgatgtagag
     1561 tccgagggcg gtgatgatga agatgaacgc gagtgcgatc tgattcgttc tccggcgcac
     1621 gaaaggtccc ttctctgccg ctactcccgc gccgtctcgt tcgagagttg cggggcttcg
     1681 ctctcgccgt tctccgttac ccgttgcaat atcgcctgtg gcacggcctg cacgcggacg
     1741 cctggcgcga gctcgagcgt gaggcgaatc gggcccgtct cgggcacatc gatcgccgcg
     1801 accgtcgcga agatgccggc actcgtcagc acttcatcgc caacgcgcaa atcagacatt
     1861 tcgcggcgct gctgcttctg ctgcttgagg acaggccgca acaggatgaa gtagaacgcg
     1921 acaaacaacg cgatgaggaa gaacatcaat tccatgggcg tgcctcgcta acggcaggtt
     1981 gccagcgcct ctcgagccac gtgcacggcc tcgtcgcgcg tgcgcgcaag ccccaagccc
     2041 tgggcttcgt ccactgcccg aaggacgcgg ccgacaagcg gcccgggcgg caaacgaaac
     2101 tcctccatga tgtcgtgccc cgtcaagagg cggtgcctcg tgataccgac gcggttccaa
     2161 cgccaacggt ccacaacgta agcaatgtac gctacatggc tcataaagtc gcgtccttcg
     2221 atgcgagggc cccgcgccgc catgtgatcg gcaagactga ggacgagaat cgcgatcgag
     2281 gtgtcgc
//
LOCUS       DATMBB010000009         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_30884, whole genome shotgun sequence.
ACCESSION   DATMBB010000009 DATMBB010000000
VERSION     DATMBB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30884"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /gene="pstA"
                     /locus_tag="VNL92_00180"
     CDS             complement(<1..864)
                     /gene="pstA"
                     /locus_tag="VNL92_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWO93164.1"
                     /translation="MAATERASFLRATGRVARRKLISSASGWVFLAASMLGVMVLVVL
                     AWDTIAQGWARLSWDFLTSYPSRFADRAGLRSSILGWVWVLGLTIVMVIPVGIGTAVW
                     MEEFAPRNRLLEVVRLNVSNLAGVPSIIYGILGLAVFVRFLDLGPSILAGALTLALMI
                     LPMTIIASQEAIRQVPPSIRDGSFAVGATQWQTVWHHVLPGAAPGIITAIILAVSRAA
                     GETAALIMIGASTFIAFDNTSMFDSFTTVPIQIYNWTVRPQQAFRENAAAGIIVLMVA
                     VLGFNLIAAIIR"
     gene            complement(864..1799)
                     /gene="pstC"
                     /locus_tag="VNL92_00185"
     CDS             complement(864..1799)
                     /gene="pstC"
                     /locus_tag="VNL92_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWO93165.1"
                     /translation="MTNDPAALRSGSLKKRPLGRPNELAIRALLTLCAAISLATTIAI
                     VVVLLEETVGFFENVSFGDFLLDTEWQPDFEPRTYGVWELVVGTLNVVVWSLVIAVPL
                     GLSAAIYLSEYAPNRVRSILKPVLEALAAIPTIVYAYFALTFITQDALEPLLEDRIGI
                     FNSLSASIVLAALILPTIASISEDAMHNVPRDLREAAYGLGATRFEVATRVVVPAALS
                     GIVASILLAIARVVGETMIVAVAAGSTPNLTLSPLESIQTMTGYMLQIGLGDAARGTV
                     QYQSIFAVGLMLFLTTLIFNLAAEFVVRRFREQYD"
     gene            complement(1910..>2902)
                     /locus_tag="VNL92_00190"
     CDS             complement(1910..>2902)
                     /locus_tag="VNL92_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWO93166.1"
                     /translation="VLVVALTACGDDDDDDDDDDGEETPAVTVTTEEGDGEAEAGAID
                     YGELGGEIRIDGSSTVFPISEAVAEEFQQVADTRVTVAFSGTGGGFERFCRGEIEISD
                     ASRPIEQDEIDACAAEGIDDIVEIQVGIDALTVMVHPENDFVECLTVQQLHDIFKTGG
                     FTQWSEVDPSFPAENIVLFYPGTDSGTFDYFVEAIIEGVDENATHRGDGTSSEDDNVL
                     AQGIAGENNSIGYFGFAYYLEAGDQLKAVAVDGGEGCVEPSFDAALDGSYQPLSRPLF
                     IYTRESLLQDNPEVLGFVNFYLENMQDLVPEVGYITLPDDLYQEQVAKIEPFLG"
BASE COUNT          562 a          929 c          929 g          482 t
ORIGIN      
        1 gcggataatc gcggcgatga ggttgaagcc gagaacggcg accatgagca cgatgatgcc
       61 cgcggccgcg ttctcgcgga acgcctgttg cggtcgcacg gtccagttgt agatctgtat
      121 gggcaccgtg gtgaaactgt cgaacatgct cgtgttatcg aaggcgatga acgtcgaggc
      181 gccgatcatg atgagcgccg cggtctcgcc ggccgcacgg gatacggcga ggatgatcgc
      241 ggtgatgatt ccgggtgcgg cgccgggaag cacgtgatgc cataccgtct gccactgcgt
      301 cgcaccgacc gcgaacgagc catcgcgaat gctcggcggg acctggcgga tggcctcctg
      361 ggacgcaatg atcgtcatcg gtagaatcat gagcgcgagc gtcagtgctc ccgccaggat
      421 gctcgggccg aggtcgagga agcgcacgaa gacggcgagg cccaggatgc cgtagatgat
      481 gctcgggaca cccgcgaggt tcgacacgtt gagccgcacc acctcgagca agcggttgcg
      541 cggcgcgaac tcctccatcc aaaccgcggt accgatgccg accggaatca ccatcacgat
      601 ggttagcccg agaacccaaa cccacccgag gatcgacgag cgaagcccgg cccggtcggc
      661 aaagcgcgac ggatacgacg tcagaaagtc ccagctgagc ctggcccaac cctgcgctat
      721 cgtgtcccag gccagcacca cgagcaccat gacaccgagc atgctcgcgg cgaggaatac
      781 ccagcccgat gccgaggaga tgagcttgcg gcgcgcaact ctccccgtcg cccgcagaaa
      841 gctggcccgc tccgtcgcag ccatcagtcg tactgctccc ggaaacgccg gacgacgaat
      901 tcggccgcga ggttgaagat gagcgtcgtc aggaacagca tcagtccgac ggcaaagatc
      961 gactggtact gaaccgtccc gcgcgcggca tcgcccaggc cgatctgcag catgtagccg
     1021 gtcatcgtct ggatgctctc cagaggagat aacgtcaggt tcggggtgga gcccgcggca
     1081 accgccacga tcatcgtctc tcccaccacg cgggcgatcg ccaggagaat ggaagccacg
     1141 atgccgctga gcgccgccgg cacgacgacg cgcgtggcca cctcgaaccg cgtggcgccg
     1201 agcccgtagg ctgcctcccg aaggtcacgc ggcacgttgt gcatcgcatc ttccgagatc
     1261 gaggcgatgg taggaaggat gagcgccgcc agcacgatgc ttgccgaaag actgttgaag
     1321 atgccgatac ggtcctccag caggggctcg agcgcgtcct gggtgatgaa cgtgagcgcg
     1381 aagtaggcgt acacgatcgt cgggatggct gccagcgcct cgagtaccgg cttcaggatg
     1441 ctacggacgc gattcggggc gtattcgctg aggtagatgg cggccgaaag cccgagcggc
     1501 accgcgatga cgagcgacca tacgacgacg ttgagcgtgc ctaccacaag ctcccacacg
     1561 ccataggtcc gcggctcgaa atcgggttgc cactcggtat ccagcaggaa gtctccgaac
     1621 gaaacgtttt cgaagaatcc gaccgtctct tcgagaagga cgacgacgat cgcgatcgtc
     1681 gtggctagcg agatcgcggc gcagagagtg agcagagcgc ggatggcgag ctcgttgggc
     1741 cgtccaaggg gccgcttctt cagcgagccg gatcggaggg ctgctggatc attcgtcacg
     1801 aggaactccg tcggcacatg gcgtgggtcc cgatcagcga accgaacgga gtggcgagag
     1861 acggtctcgg ccgtccctcg ccactggttg ctcgacccgc gcggggatgc tagcccagga
     1921 acggctcgat cttcgccacc tgttcctgat acaggtcatc cggcagcgtg atgtagccaa
     1981 cttccgggac gaggtcttgc atgttctcca ggtagaagtt cacgaagccc agcacctcgg
     2041 ggttgtcctg caacaggctc tcccgggtgt agatgaacag cgggcggctc agcggctgat
     2101 acgaaccgtc gagcgctgca tcgaatgacg gctcaacgca tccttcgccg ccatcgaccg
     2161 cgaccgcctt cagctggtcg ccggcctcaa gatagtaggc gaacccgaag tagccgatcg
     2221 agttgttctc gcctgcgatg ccctgcgcaa gcacgttgtc gtcctcagag gacgtgccat
     2281 cgccgcggtg tgtggcgttc tcatcgacgc cctcgatgat ggcctcgacg aagtagtcga
     2341 aggttcccga gtctgtgcct ggatagaaca ggacgatgtt ctcggccggg aacgagggat
     2401 ccacctcgct ccactgcgtg aacccgccgg tcttgaagat gtcgtgcagc tgctggaccg
     2461 tgaggcattc cacgaaatcg ttctccgggt gcaccatcac ggtgagggcg tcgatgccga
     2521 cctggatctc gacgatgtca tcgatgccct cggcggcgca ggcgtcgatt tcgtcctgct
     2581 cgatcggccg cgacgcatcg ctgatctcga tctcgccgcg acagaacctc tcgaatccgc
     2641 cgcccgtgcc ggagaaggct accgtgacgc gggtatcagc cacctgctgg aattcctccg
     2701 cgacggcctc ggaaatgggg aacacggtcg acgagccgtc gatccggatc tcgcccccca
     2761 actcgccgta gtcgatcgcg ccggcttccg cctctccgtc gccctcttcg gtcgtcaccg
     2821 tgacggccgg ggtctcctct ccgtcatcgt cgtcatcatc atcatcgtcg tcaccgcacg
     2881 cggtcaacgc gacgacgagc ac
//
LOCUS       DATMBB010000010         3845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_28863, whole genome shotgun sequence.
ACCESSION   DATMBB010000010 DATMBB010000000
VERSION     DATMBB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28863"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VNL92_00195"
     CDS             <1..936
                     /locus_tag="VNL92_00195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWO93167.1"
                     /translation="EVITSAFTFSSCAHVVVHLGATPVLADICEDDWNVDPADIERRI
                     TSGTRALMPVHYGGQPCRMDDIVALAKARGLFVLDDAAHAIGAAYRGLPVGSLADATA
                     FSFYATKNVAMGEGGALTTADAAIDERARRLALHGMNRDAWQRYETGGSWYYEVVEAG
                     YKYNLTDIQSALGLAQLTRLDWMTARRAELAARYTAAFASMDELGLPAVRPEVRHAWH
                     LYAIRIRPEALRIDRNRFIALLAERGIGTSVHFIPVYRHPFYRDTFGLRPEDFPVTER
                     VYQTCISLPLYPGMTDAEADRVIEAVGDIVRKEQR"
     gene            933..1556
                     /locus_tag="VNL92_00200"
     CDS             933..1556
                     /locus_tag="VNL92_00200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWO93168.1"
                     /translation="MKGGNSRAERRLFDLVAAVLGTAILTPLFVLIAIALRLDSPGPV
                     LFRQVRIGRHGRPFLVYKFRTMYSSSAPRPHSAPQTTEAVDFESFVFTPDTADPRVTP
                     IGRVLRKTSMDELPQLLNVIRGEMALVGPRPEIPELVALYPAAFHERHSVLPGITGLA
                     QVSGRSDLTYAQTIRYDLAYVKMRSFRNDLAILGRTIGAVLRKSGAR"
     gene            1608..3476
                     /locus_tag="VNL92_00205"
     CDS             1608..3476
                     /locus_tag="VNL92_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HWO93169.1"
                     /translation="MQRWFRHTEGRAETLLMGPGRDALIVVAAYVAALLLRFDFHVPN
                     DSWRFFAASAALIVLVYVVANHFLGIYRTIWAYASSRDAMRLAESTGIATIALFALNV
                     TLPERHVPLSVVVVGGAFVFLGMSGTRIWSKLWEPSLLRRNPAATRVLIAGAGNTGQL
                     VAREFLHNRSWNYTPVCFVDDNPRRQGQRIHGVPVLGTRGDIPTLVRKHNIDVVAIAM
                     PSADGAVMRELISMCQLLRVPVRMVPGLPEIVSGTASPGDLREITIEDLLSREPIEID
                     YQTCAQKLQGKIVLITGAAGSIGSELARQVARFEPAALHLLDNNESELFDLSMELLAE
                     QRDTEIRSWIADVASAPKIRTIFAKARPQVVFHAAAYKHVPLMEEHPDEAFRVNVLGT
                     LNVCQAAHEQGADRLLFVSTDKAVNAAGVMGASKRIGECIVQAMAERSDTVFCAVRFG
                     NVIGSRGSVVPLFWKQIERGGPVAVTHPEMTRYFLTVSEAVTLLIQTAAFAGQGQILM
                     LDMGDEIRIVELAERMIRMRGLDPGRDIQIVFTGLRPGERIREELVAEGERVQPTSHP
                     KVLLTLPPGKVPWERLHAGILALAEELSSPPEDFANRLHALARIDRKDAEVHLPPA"
     gene            3499..>3845
                     /locus_tag="VNL92_00210"
     CDS             3499..>3845
                     /locus_tag="VNL92_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93170.1"
                     /translation="MSPGAAGTISAEAFVHPTAHIDDGVTIGRGTRVWHEVHIRERAS
                     IGEECILGKSVYIDREVTVGNRVKIQNRASLYRGVTIEDGVFIGPHVIFANDRMPRAI
                     NPDGSLKSDEDWV"
BASE COUNT          658 a         1286 c         1234 g          667 t
ORIGIN      
        1 gaggtcatca cgagcgcctt caccttctca tcctgcgcac acgtcgtagt tcaccttgga
       61 gcgacgccgg tgctcgccga catctgcgag gacgactgga acgtcgaccc ggccgacatc
      121 gagcgtcgca tcacgtccgg aacgcgggcc ctgatgcccg tgcactacgg cggacagcca
      181 tgccggatgg acgacatcgt cgcgctggca aaggcgcgcg gcctcttcgt gctcgacgat
      241 gccgcacacg ccatcggcgc tgcctaccgc ggcctcccgg tcggcagcct cgctgatgcc
      301 accgccttca gcttctacgc gacgaagaac gtcgccatgg gcgaaggggg cgcgctgacc
      361 acggccgatg cggcgattga cgaacgagcg cgcaggctgg cccttcatgg gatgaaccgc
      421 gatgcctggc agcgctacga gaccggaggg tcgtggtact acgaggttgt ggaggccggc
      481 tacaagtaca acctcaccga catccaatcg gcgctcgggt tggcgcagct cactcgcctc
      541 gactggatga cggcccgacg cgcggagctc gccgcccggt atacagcagc gttcgcatcg
      601 atggacgagc tcggtctgcc ggcggtccgg cccgaggtgc gtcacgcgtg gcatctctac
      661 gccattcgca tccggccgga ggcgctccgc atcgaccgca atcgcttcat cgccctgctg
      721 gccgagcgag gcatcggcac atcggtccac ttcatccccg tgtaccggca tccgttctat
      781 cgcgacacgt tcggtctgcg gccagaggat tttccggtca cggagcgcgt ctaccagacc
      841 tgcatctccc tgccgctgta tcccgggatg acggatgcgg aagcggaccg cgtgatcgag
      901 gcggtgggcg acatcgttcg gaaggagcag cggtgaaggg cgggaactcc cgcgccgaac
      961 gccggctgtt cgacctggtt gcggcggtcc tcgggaccgc tatcctcacc ccgctgttcg
     1021 tcctcatcgc catcgcgctg cggctcgatt cgccggggcc cgtgctgttt cggcaagtgc
     1081 gcatcggccg gcatggccgt ccgttcctcg tgtacaagtt tcgtaccatg tactcatcga
     1141 gcgccccccg cccgcacagc gcgccccaaa cgacggaggc cgttgacttc gagagcttcg
     1201 tcttcacgcc ggacaccgcg gacccgcgtg tgacaccgat cgggcgcgtg ctccgcaaaa
     1261 cgagcatgga cgaactgccg cagctcctca acgtgattcg cggcgagatg gcgttggtag
     1321 gcccgcggcc ggagattccg gagctggtcg cactgtaccc tgcggcgttc cacgagcggc
     1381 atagcgtgct gccagggata acgggcctcg cccaagtctc gggaaggtcc gatttgacct
     1441 atgcgcagac cattcgctac gatcttgcat acgttaagat gcgctcgttc cgcaacgacc
     1501 tcgcgatcct cggtcgaacc atcggtgccg tgctgaggaa gagcggagcc cgctgaggcg
     1561 ggcgagagtg acgggcgcgg ggccgccgcg cgggagctag gaaaccggtg cagcgctggt
     1621 tcaggcacac tgaggggcgg gccgagaccc tgcttatggg ccctggtcgt gacgccctca
     1681 tcgtcgttgc ggcctacgtc gcggcccttc tcctccggtt cgatttccac gtcccgaacg
     1741 attcgtggcg gttcttcgcc gcgtcggccg ccctcatcgt gctcgtctac gtcgtcgcta
     1801 accacttctt gggcatctat cgaacgatct gggcctacgc ttcctctcgt gatgcgatgc
     1861 gactcgcaga gtcgacggga atcgccacga tcgcgctctt cgccctcaac gtaaccttgc
     1921 cggagcggca cgtgccgctt tcggtggtgg tcgtcggcgg cgcgttcgtc tttctcggca
     1981 tgtcgggcac gcggatatgg agcaagctgt gggaaccgag cctcctgagg cgcaatcccg
     2041 cggcgacccg cgtcctcatc gccggcgcgg gcaataccgg acagctcgtg gcgcgcgagt
     2101 tcctgcacaa ccggtcgtgg aactacacgc cggtctgctt cgtcgatgac aacccgcgac
     2161 gccagggaca gcgcatccac ggtgtgcccg tgctgggaac gcggggcgac atcccgaccc
     2221 tcgttcgcaa gcacaacatc gatgtggtcg cgattgcgat gccctccgcc gatggcgccg
     2281 tcatgcgcga actgatcagc atgtgccagc tgcttcgtgt gcccgtgcgg atggtccctg
     2341 gtctcccaga gatcgtatcg ggtacggcga gcccgggcga cctgcgcgag atcacgatcg
     2401 aggacctcct cagccgcgag ccgatcgaga tcgactatca aacgtgcgcg cagaagctcc
     2461 agggcaagat cgtcctcatc accggcgcgg ctggatcgat cggctccgag ctggcccgcc
     2521 aggtcgcgcg gttcgagccg gccgccctgc acctcctcga caacaacgag agcgagctgt
     2581 tcgacctttc gatggagttg ctggccgagc agcgtgacac cgagattcgg agctggatcg
     2641 ccgatgtcgc gagtgcaccg aagatccgaa ccatcttcgc gaaggcccgg ccgcaggtcg
     2701 tgttccacgc ggcggcatac aagcacgtgc ccttgatgga ggagcatccg gacgaggcgt
     2761 tccgtgtgaa cgtgctcggc acgctgaacg tctgccaggc cgcgcacgaa caaggcgccg
     2821 accgcctcct gttcgtgtcc accgacaaag cggtgaacgc ggccggcgtg atgggcgcct
     2881 caaagcgaat cggcgagtgc atcgtccaag cgatggcgga acgaagcgac accgtcttct
     2941 gcgccgtccg cttcgggaac gtgatcggaa gccggggcag cgtcgtgcct ctcttctgga
     3001 agcagatcga gcggggcgga cccgtcgccg tcacgcaccc cgagatgacg cgctatttcc
     3061 tcaccgtctc ggaagcagtc accctgctca tccagaccgc cgcgttcgcc ggccagggtc
     3121 agatcctcat gctggatatg ggcgacgaga tccgcatcgt ggagctcgcc gagcggatga
     3181 tccgcatgcg cggcctggat ccgggtcgcg atatccagat cgtgttcacg ggcttgcggc
     3241 ccggagaacg cattcgagag gagctcgtcg ccgagggaga gcgtgttcag ccaacatcac
     3301 atcccaaggt gctgctgacc ctccctccag ggaaggttcc ttgggagcga ttgcacgcgg
     3361 gcatcctcgc gctcgcggag gagctgagct ctccgccgga ggatttcgcc aaccgcctcc
     3421 atgccctcgc acgcatcgat cgcaaggatg ccgaggtcca tctgcccccg gcataacaac
     3481 gaagacaagg ggatcgccat gagcccagga gctgcgggca ccatcagtgc cgaggcattt
     3541 gtccacccta ccgcacatat cgatgacggc gtcaccattg gccgcggcac gagggtgtgg
     3601 cacgaggtcc acatacgcga gcgggcgtcc atcggcgagg aatgcatact cggcaagtcc
     3661 gtctatatcg atcgggaggt cacggtcggg aatcgggtca agatccagaa tcgagcgtcg
     3721 ctgtatcgcg gcgtaaccat cgaggacggc gtcttcatcg gcccgcatgt catcttcgcg
     3781 aacgatcgca tgccgcgcgc catcaatccg gacggctccc tcaagtccga cgaagactgg
     3841 gtgat
//
LOCUS       DATMBB010000011         5770 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_231771, whole genome shotgun sequence.
ACCESSION   DATMBB010000011 DATMBB010000000
VERSION     DATMBB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231771"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VNL92_00215"
     CDS             complement(<1..458)
                     /locus_tag="VNL92_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWO93171.1"
                     /translation="MPSRILVVEDEAVVAEVVERYLRRDGHDVSVAFDGEAALAAIDR
                     ERPDLIVLDVMLPAIDGLEVCRRVRAVSETPIVMLTARGDEIDRLLGLGLGADDYVTK
                     PFSPRELAARVKAVLRRTSATARPESQTLRFGDLRIDARARTVEDARGAIG"
     gene            710..925
                     /locus_tag="VNL92_00220"
     CDS             710..925
                     /locus_tag="VNL92_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93172.1"
                     /translation="MMRFYLKACPRCSGDLMLAEAELGEPELVCIQCGYSKGVSALRE
                     AGRRRREMAVAGAGAKRSERGGGFHTT"
     gene            959..2083
                     /locus_tag="VNL92_00225"
     CDS             959..2083
                     /locus_tag="VNL92_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93173.1"
                     /translation="MYFPMLIVRNVLTRKARTLLTGMAIAVSIMTVLTMGVLTESLER
                     TATSIMETGQADFTIAQKGVSDILNSALDEEEVARVAEYEGVTAAVGVLLAIDELDAD
                     HPFFLQIGIEPDQLAAFGVTVIEGRSYARTAEDEILLGYRAARDLDKAVGDTIVIDDD
                     TYRIVGLFSTGQVYGDSGAMLPLTTLQAREQKPGNVTLLFVQTEDKDPEAIDSLRTRM
                     EDDLPLLSTARTATEFGKLDRNLALISAADSGVTIMALVIGGVIVMNTMMMSVFERTR
                     EFGVLRAIGWSRWRILFDVLGEAILIALIGAGLGVGLGFLAIRGLNEAPELVGVFHAH
                     YTTGVFARAVALAIGMAIVGGLYPGVRAALLRPMEALRHE"
     gene            2076..2798
                     /locus_tag="VNL92_00230"
     CDS             2076..2798
                     /locus_tag="VNL92_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93174.1"
                     /translation="MNERAGSPAIQTFDLTKTYDGGAVRALDGVTLSVDGGEWVTIAG
                     PSGCGKSTLLHLLAALDEPTSGRIVVQGRDLRTRGDLDRYRRLDIGLVFQLHNLLPNL
                     TALQNIEVAMLGNGLSQREQRRRATALLEEFELAGKARFTPPRLSGGERQRLAIARAL
                     ANEPGIMLADEPTGSLDSRSADRVLGLFRRIRRERGVTVVMVTHDAHVATAADRVIEM
                     RDGRIVGMEAPVREAVATQRSP"
     gene            complement(2815..3060)
                     /locus_tag="VNL92_00235"
     CDS             complement(2815..3060)
                     /locus_tag="VNL92_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HWO93175.1"
                     /translation="MIGISAQVSLYPLRQEHVSPAIDEAIAILRRAELDVTVGPMSTV
                     VAGDDDRVFDALKQAFRAASQRGDVVLVVTFSNACPV"
     gene            complement(3057..3719)
                     /locus_tag="VNL92_00240"
     CDS             complement(3057..3719)
                     /locus_tag="VNL92_00240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93176.1"
                     /translation="MAEQPLTPFDTFAREYDAWYESPYGASVLQREVECLRPLLQPTG
                     RALEVGVGTGRFAVPLGIAHGVDPSLPMLRMAAGRGIVSVCGVGEMLPYRDGVFDAVL
                     LAASLEFVAHARRVLEEARRTLRDDGTLVVGIINRESAWGGAYQQMATQGDPIFSQAR
                     FWTAQEAMLLGEAAGFSFVRARSTLLGPPFVIAEPDSREGAVAEAGFVAIAFRKREEE
                     TS"
     gene            3983..4462
                     /locus_tag="VNL92_00245"
     CDS             3983..4462
                     /locus_tag="VNL92_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWO93177.1"
                     /translation="MATESEERERRPWPFGPRSVRRWFEEADPFRVRFERRWPFISDF
                     LAQEPWEPAVDMIEKDGKLIVKADLPGVKREDIDVTVERGALRIRAERRQEKDVREED
                     YRRVERTYGRYERVLPLPEGIDPESISASCKDGVLEVVMPLPKAEAAEAKKVPIGGE"
     gene            4493..5665
                     /locus_tag="VNL92_00250"
     CDS             4493..5665
                     /locus_tag="VNL92_00250"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HWO93178.1"
                     /translation="MLNDHWRRTLRELADIEDDDLPFLTVTMDLRPDGAARRESLTWL
                     SQAMLDRDADRKSLPYHGDALKTFEDDLAWVRDYVGDFRGGRYDPASKGIVFFACGAR
                     GIRRVIELPVPIENRLAVAGRPVLIPLAAAVASYPDYLVAPVDADSCRLFAVSMKRVW
                     DRAEHQGDTADLSWTAREGGWSLKRYQRHIDHWRSAFAEESMAMIVQELSRVDAPYLV
                     LIGEEWAVAELRRHLPEALSAEVVFGGHVMVDLANEAQVVGEAEPVVQKEYERRVREQ
                     GDRIVAAASEGRPAAMGLGPVVRALEEGAVSLLVLPPSLEIHGWRCTSCGHLSEGESP
                     GSCPLCRQQTTPVDMGEVILREALRQDAQIFFLSSHAELGSYGGVGALLRFPVSVR"
BASE COUNT          980 a         1769 c         2005 g         1016 t
ORIGIN      
        1 cctatggctc cgcgggcgtc ctcgacggtt cgagcgcgcg cgtcgatccg cagatcgccg
       61 aagcgaagcg tttggctctc gggcctcgcc gtcgctgagg tgcggcggag cacggctttg
      121 acgcgagcag cgagctcccg agggctaaat ggcttggtca cgtagtcgtc cgcgcccaag
      181 cctagcccga ggagcctgtc gatctcgtcg cctctggcgg taagcatgac gatcggcgtc
      241 tcggagacgg cgcggacgcg gcgacagacc tcgagcccat cgattgcagg aagcatcacg
      301 tcgaggacga taaggtccgg acgttcgcgg tcgatggcgg cgagcgcggc ttccccatcg
      361 aaggcgacgg acacgtcgtg tccgtcgcgg cgcaagtagc gctcgacaac ttcggccacg
      421 acggcctcgt cttcgaccac gagtattcgg ctcggcacgc tatccctccg gtggaggcgt
      481 tcgttcctct atgacgggaa cggtacacga tggacctgtg gatgtgcact ccgacctcgg
      541 cacgaggcga cgcgcggaac gatgcgagcg cgaccctctg cgcgtccgtg ccaagcggcc
      601 cggtcggaca cgtgccgtgt ggcgtcctgg ttttgcgccg tacggcacac gccggcccat
      661 gaggacgcgc ctacgatgac cgcgtcgtgg cctcatacga gaggagggaa tgatgcggtt
      721 ctacctcaag gcctgtccac gctgctcggg tgatctcatg ctcgccgagg cagagttggg
      781 cgagccggag ctcgtctgca tccaatgtgg ctattcgaag ggcgtcagcg ccctgcggga
      841 ggcggggagg cgccggcgag aaatggctgt cgccggcgcg ggcgcgaaac gaagcgaacg
      901 gggcggcggg tttcatacca cctaaggccg gtgggttcgc taacgcttga ggtgaaccgt
      961 gtacttcccc atgctcatcg ttcgcaacgt gctgacccgg aaggcgcgca cgttgctgac
     1021 cggaatggcg atcgcggtaa gcattatgac ggtcctgacg atgggcgttc tcacggagag
     1081 cttggagcgg acggccacgt caatcatgga aacggggcag gccgacttca cgatcgcgca
     1141 gaagggcgtc tccgacatcc tgaacagcgc cctcgacgag gaggaggtcg cgcgtgtcgc
     1201 tgagtacgaa ggtgtgaccg cggccgtcgg cgtcctcctc gcgatcgacg aactcgacgc
     1261 cgaccaccct ttcttcttgc agatcggcat cgaaccagac cagctcgccg cgtttggggt
     1321 caccgtcatc gaagggcggt cgtacgcgcg tacggccgaa gacgaaatct tgcttgggta
     1381 ccgcgctgcc cgcgacctcg acaaggctgt gggtgacacc attgttatcg acgatgatac
     1441 ttaccgtatc gtcggattgt tctccacggg tcaggtctac ggcgattcgg gtgccatgct
     1501 gcccctcacg accctccagg cccgggagca gaagcctggg aatgtcacct tgctcttcgt
     1561 gcaaacggaa gacaaggatc ctgaagcgat cgacagcttg cgaacgcgga tggaggatga
     1621 cctcccgctg ctctccacgg cccgcacagc caccgagttc ggcaaactcg accgcaatct
     1681 agcgctgatc agcgctgccg acagcggtgt taccatcatg gcgttggtga tcggcggtgt
     1741 catcgtcatg aacacgatga tgatgtcggt tttcgagcgg acgcgggagt ttggtgtgct
     1801 gcgggcaatc gggtggtcgc gctggcgcat cttgttcgac gtactgggcg aggccattct
     1861 catcgccctt atcggtgctg ggctcggtgt aggcctcggc ttcctggcga ttcgtggcct
     1921 caacgaggca ccggagctgg tcggcgtttt ccacgcccac tacacgacgg gcgtcttcgc
     1981 tcgcgcagtg gcgcttgcca tcggcatggc catcgtcggc gggttgtatc ccggcgtccg
     2041 agcagcgttg ctgcggccga tggaagccct gcgccatgaa tgaacgcgcc ggttccccag
     2101 cgatccagac gttcgacctc acgaagacct acgatggagg cgcagtgcgc gccttggacg
     2161 gcgtgacgtt gagcgttgat ggcggggaat gggtcaccat cgccgggccc tcgggttgcg
     2221 ggaagtcaac gctgcttcat ctgctcgcgg ctcttgacga accgacctcg ggacgtatcg
     2281 tcgtgcaggg ccgcgatctc cgcacacggg gcgacctcga ccgctaccgc cggctcgaca
     2341 taggccttgt ctttcagttg cacaatctcc tgcccaactt gacggctctc cagaacatcg
     2401 aagtcgcgat gctgggcaac gggctgagcc agcgcgagca gcggcgccgg gcgacggcac
     2461 tgttggagga gttcgagctg gccggcaagg ctcggttcac gcccccgcgc ctttccggcg
     2521 gcgagcgtca gcgcctcgct attgcgcggg ccttggccaa cgagcccggc atcatgctcg
     2581 ccgacgagcc cacaggcagc ctcgactccc ggtctgcgga ccgggtgctg ggtttgttcc
     2641 ggcgaattcg gcgtgaacgc ggtgtcacgg tcgtgatggt gacgcacgac gcgcacgtgg
     2701 ccaccgcagc agaccgcgtc atcgagatgc gcgacgggcg tatcgtgggc atggaagcgc
     2761 cggtccgaga ggctgtcgcg acgcagcggt cgccttagcc cgtcggaccg aaggctacac
     2821 ggggcatgca ttcgagaacg tgacgacgag cacgacatcg ccgcgttggc tggccgcgcg
     2881 gaaggcctgc ttcagcgcat cgaagacgcg gtcgtcatcg ccggcgacga ctgtgctcat
     2941 ggggccgacc gtgacgtcga gctcggccct gcggaggatg gcgatggctt cgtcgatggc
     3001 aggcgacacg tgctcctggc ggaggggata caggctgacc tgtgcggaga ttccgatcat
     3061 gaggtttcct cctctcgctt tcggaaggcg atggcgacga agccggcctc tgcgaccgct
     3121 ccttctcgcg agtcaggctc ggcaatgacg aaaggcgggc cgagcagggt gcttcgcgcc
     3181 ctcacgaatg agaatccggc ggcttcgccc agcagcatcg cctcctgggc ggtccagaac
     3241 cgtgcctggg aaaagatggg gtcgccctgc gtcgccatct gctgatacgc cccgccccag
     3301 gcgctctccc ggttgatgat cccgacgacg agcgttccat cgtcgcggag cgttcgccgc
     3361 gcctcctcga gtactcggcg cgcgtgggct acgaattcga ggctggcggc gagcaggacc
     3421 gcatcgaaca cgccatcgcg gtagggtagc atctcgccta cgccgcagac agagacgatg
     3481 ccgcggcccg cggccatgcg gagcatcggc aatgacggat cgacgccgtg ggcaatgccc
     3541 aaaggcacgg cgaatcgtcc cgttcccacg ccgacttcta acgcgcgtcc agtaggttgc
     3601 agcaggggcc gcaggcactc cacctcgcgc tggaggacgc tggcgccgta cgggctttcg
     3661 taccaagcgt cgtactcccg cgcgaaggta tcgaacggtg tgagcggctg ctcagccatg
     3721 gcgcggcgcc tccctcgggg ctcgtttatc gctcgtcgca tccccattat tgcggcccac
     3781 cacgagccga aggaagaacc tcgcgtcgct ggtctttcgc ccttcgtccg gtgcgctcaa
     3841 gggtgtgtga catcgtgaat ccgaaaggtc cggcggaaag agccaaacgg ccccttcaaa
     3901 cgtagccgcg gatggtggaa attggatgta tcgggggttt cttgtagtgc gtccaaggcg
     3961 gaagaaaagg agcgtcgtca aaatggcaac cgagagcgaa gagcgcgagc gtcggccgtg
     4021 gccgttcggg ccacgatctg tccggcggtg gttcgaggag gccgatccct tccgtgtacg
     4081 tttcgaacgc cgctggccgt tcatctctga cttccttgct caggagccgt gggagccggc
     4141 cgtcgacatg atcgaaaagg acggcaagct cattgtgaaa gccgatttgc ccggcgtgaa
     4201 gcgtgaggat atcgacgtca cggtggagcg cggagcgctg cgcattcgcg ccgagcgccg
     4261 gcaggagaag gacgttcgcg aggaggacta ccgccgggtc gaacggacct acggccgcta
     4321 cgagcgggtg ctgccgctgc ccgaaggcat cgacccggaa tcgatttctg ccagctgcaa
     4381 ggatggtgtt ctggaggtcg tgatgccgct gccaaaggct gaggcggctg aggcgaagaa
     4441 ggtgccgatt ggtggcgagt agagctgttg agggggccga aggcggtggg agatgctgaa
     4501 cgaccattgg cggcggacgc tgcgggagct tgccgacatt gaggacgacg acctcccatt
     4561 cctaacggtg accatggacc tccggccgga tggcgcggca cgtcgcgaga gcctaacgtg
     4621 gttgagccag gccatgctcg atcgcgacgc cgaccggaag tcactgccct accatgggga
     4681 tgccctgaag acgtttgagg atgacctggc gtgggtgcgg gactatgtgg gggactttcg
     4741 aggcggcagg tacgatccgg cgagcaaagg catcgtattc ttcgcctgcg gagcgcgcgg
     4801 catacgcagg gtcatcgagc tcccggtgcc gatcgagaat cgtctcgctg tcgcgggccg
     4861 gccggtgctc ataccgttgg ccgccgccgt cgcatcgtat ccggactacc ttgtagcgcc
     4921 cgtcgatgcc gactcgtgcc gtttgtttgc tgtgtcgatg aagcgagtgt gggaccgggc
     4981 agagcaccag ggcgacacgg ccgacctttc gtggacagcc cgcgagggcg gatggtcact
     5041 gaagcgatac cagcgccata tcgaccactg gcggagcgcc ttcgctgagg agtcgatggc
     5101 tatgattgtg caggagctga gccgtgtcga cgcgccctac ctcgtgctca ttggggagga
     5161 gtgggctgta gccgagcttc gccggcatct cccggaggcg ctgagcgccg aggtggtgtt
     5221 cggcggacac gtcatggtgg acttggcgaa cgaggcgcag gtcgtcggcg aagctgagcc
     5281 ggtagttcag aaggaatacg aacggcgagt ccgcgagcag ggagaccgga ttgtcgcggc
     5341 cgctagcgag ggccggccgg cggcgatggg gctgggaccg gtcgttcgcg cgctggagga
     5401 gggggcggtg agcctactcg tgttaccgcc ctcgctcgag atccacggat ggcgatgcac
     5461 cagttgcggg cacctctcgg agggcgaatc gcccggcagc tgtccgctct gccggcagca
     5521 aacgacgccg gtcgacatgg gggaggtcat cttgcgcgag gccttgcgcc aggacgcgca
     5581 gatattcttc ctctcgagtc acgccgagct cggctcctac ggcggggttg gggcgctgct
     5641 ccgctttccc gtgtcggtgc gataggaggt cacgcggaag cgcagtccgc ggctacggcc
     5701 atccggcaga ccggggaatc acttcctgaa ggtggcaggt tctggaccac gtctatgacc
     5761 ggcccacggc
//
LOCUS       DATMBB010000012         6574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_231755, whole genome shotgun sequence.
ACCESSION   DATMBB010000012 DATMBB010000000
VERSION     DATMBB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6574
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231755"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VNL92_00255"
     CDS             <1..750
                     /locus_tag="VNL92_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWO93179.1"
                     /translation="GGRWGPSHETCLEQAQRYEDLFIPAAVVDPGETEPERIDELYAM
                     GYRGLKIIGTARAYDDRSYFPVYERAQANHMPILFHCGVIGGPIDLRTHHPRRDPESA
                     NRLLAFAERLRGMGVRDVGASRMRPFHLDTIANNFPELPIIGAHLGGTGNYDEAASVA
                     RWRLNVAFDMSGGEVIERHAVERRLIGFEITVEKLMFGSDCAADDIQDHVDRFQSIFD
                     ELGLDDDARERIWYGNAAEMFGLQEPAWAAE"
     gene            773..1612
                     /locus_tag="VNL92_00260"
     CDS             773..1612
                     /locus_tag="VNL92_00260"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93180.1"
                     /translation="MRRTVIPEAHPTQPEPDRTQTGDRANLLILAVGVGLFLFLTIEP
                     TQAWLLLLITGLALLGMGALARAEAVYRSEMSGEAPAELRVSDSVFSLIIPTLWVFGG
                     GLLAEEIAGGFWSIPAAVVVAAGFGIIAHAELFSLHEESSWYQAARFALNVAAYVIAF
                     ALFATPYALELGRFAAAIFVGVAAVLMAIDLLREAPIPAVRVLLVAGAAGLLLAETRW
                     SMYYLGIEEFSSATLLLVVFYVASGLLQSQLLAKLSAATALEYALVGGAGLLIIAAAE
                     LIG"
     gene            1609..2271
                     /locus_tag="VNL92_00265"
     CDS             1609..2271
                     /locus_tag="VNL92_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HWO93181.1"
                     /translation="MTSRVLGIDLASRGRPLGYAVLDFGARIIAVGRDALDDDALLRL
                     ATHHRACRVAIDAPLFLPEGLCCLDDACPCAPLQASGLRAAEVALRALGMGGFATTKR
                     TIIPRLAERGIGLRRSFEAAGFDTAEVYPHGAKVRLFGERRPPKKGRPAGDEWLRQRI
                     ARIVRALPDRLLHHDEADAILAAYTGLLIERGEAEPLGESRDGLVWLPAGPRLAVASR
                     TA"
     gene            2281..2778
                     /locus_tag="VNL92_00270"
     CDS             2281..2778
                     /locus_tag="VNL92_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93182.1"
                     /translation="MPLEYDDLPPLARTYLAVVSLGLPSARLANDESFRIDYVCAACH
                     ALWAGQAMRYHLEGDYQPQRGFLDELRATIVDLDNAGVIIAGAGDGHLATMPFLGQDA
                     GKLTPGQIERLDLNAPPKVLDTYLAYKALDELLSHPKAYTFVMEKYGASSEIWQQLTA
                     RGYGR"
     gene            2775..3698
                     /locus_tag="VNL92_00275"
     CDS             2775..3698
                     /locus_tag="VNL92_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO93183.1"
                     /translation="MTITFGVLPAHDESGDLLGSVELAEALGFDSVWLGDHVLWHTPS
                     PDPMVLLGAIAARTSRIGIGTGIYLAALRPAAVVAKQAATLDHLAEGRFILGVGIGGE
                     NPAEFAAVGVPLSERASRLEETIRACRALWSADGQPVDFDGRYVAFRQVRFDYPPRTQ
                     GGPPIWMGGRAEGALRRAGTMADGWLAFVVDPQRYAENWRKVRAYATSAGRDPDQLTP
                     ALQLWCQLGEDDSSARAILAPAIERMYRTPYERFERYCIAGDAQTWIRRLGEFIEAGV
                     RHFNLIFAGGDVRMQMVLFMDGVRPMLRPVD"
     gene            3778..5532
                     /locus_tag="VNL92_00280"
     CDS             3778..5532
                     /locus_tag="VNL92_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93184.1"
                     /translation="MREGNYWTEVFRRRMTRRRALLAAGSGAAGAGAIGLVGCGDDDD
                     DDDDEEAGTAPTGGTAAAGDIRRGGTYVASVPIDPPTLDPYKNLSYLTQTVAGYAYSR
                     LLKFQTGPDVLPSDYLPEPDLAEALPESPDAITWTFKIRPEAVYAPGDPLQGRAVTAE
                     DVRYSYERFINTPENERKNDVIPVIDRVEVIDDKTVQFTLKQAYADFITLMASSSHLW
                     IVPPEVVESGLVESKPIGSGPFMLESYERSRQMTFAKNPGYWVMGDDGQPLPYIDRNQ
                     YLVIADAQTTVAQFTSGQLHGLTITEEQRGQIESDVSGVLIYDFLANLLNQFWFDPRT
                     YSEDLPPFNDERVRQAISMSVDRAGLIRLIFNGQGEWHNQGVPAGLSYWWLDPQGENI
                     GEGGQYYQYNPEEARKLLEAAGVPDLKVRVHYTEDGYRSIAGGFYNRIADSLPGMFRE
                     SGIEVELVPESYSAVYVPQTLAGNFDSMAWSLSTPFNFIHLYLVNYYTGTAGRDRGVR
                     DQEILDLIDRQIQTVDQEERRELIYEIQRIASVKQYFPGGVLGPSFQAVQPQIKNYFV
                     TGSYGTGTETIAKLWIDA"
     gene            complement(5612..>6574)
                     /gene="corA"
                     /locus_tag="VNL92_00285"
     CDS             complement(5612..>6574)
                     /gene="corA"
                     /locus_tag="VNL92_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWO93185.1"
                     /translation="RCVNDASGRCRFQGAVMVATSELPGEVLGENPLWLDVENPTAEE
                     MDIIARRFGFHPLSVEDALDPRNQRPKLDQYDTYRFLVFYGFSGADELDAQEINIFWA
                     SDYVVTVRHGPVESIAEARRRWSAHERGRDRTSSDLLHVILDTAVDSVLPLVDDIAEQ
                     VEELEDQVFARPRAEIATRGQQIRRRLLGLRRVLGPERDVIVALRRDPLVEEQEETIR
                     YFDDVFDHVSRAADAVDLERDMVTSVMETYLTAVSNNLNEVMKTLTIAATILMTMSLV
                     TGIYGMNFDNMPELDWALGYPFALGVMVAAALALVFFFRRRRWL"
BASE COUNT         1112 a         2211 c         2091 g         1160 t
ORIGIN      
        1 ggcgggcgct ggggcccgag ccacgagacg tgcctcgagc aggcgcagcg ctacgaggac
       61 ctctttattc ccgccgccgt ggtcgacccc ggcgagacgg agcccgagcg catcgacgaa
      121 ctctatgcca tggggtaccg cggcctcaag atcatcggga cagcacgggc gtacgacgat
      181 cgctcatact tcccggtgta cgagcgggcc caggcgaacc acatgcccat tctgttccac
      241 tgcggggtca tcggcggccc catcgatctt cgtactcacc acccgcgccg cgatccggag
      301 tcggcgaacc ggctcctggc cttcgccgag cgcctgcggg ggatgggcgt gcgcgacgta
      361 ggggccagcc gcatgcggcc cttccatctc gatacgatcg cgaacaactt tccggagctg
      421 ccgatcatcg gcgcccatct cggtggcacc ggcaactacg acgaggccgc ttccgtcgcg
      481 cggtggcgcc tcaatgtagc attcgacatg tcgggcggcg aagtcatcga acgccacgcc
      541 gtggagcggc gattgatcgg cttcgagata accgtcgaga agctcatgtt cggctccgat
      601 tgcgctgcgg acgacatcca ggaccacgtc gatcgcttcc agagcatctt cgacgagttg
      661 ggcctcgatg acgacgctcg cgagcgcatc tggtatggca acgccgccga gatgttcggc
      721 ttgcaggagc ccgcctgggc cgccgaatga cggccggccg cttcagcgtc taatgcgccg
      781 cactgtcatt cccgaagccc atccgactca accggagcct gatcgtaccc aaaccggcga
      841 ccgggcgaat ctcctcatcc ttgcggtagg cgtcgggttg ttcctctttc ttacgatcga
      901 gcccacgcag gcgtggctgc ttctgctcat cacgggactc gcgctcttgg gcatgggcgc
      961 gctcgcgcgg gccgaggcgg tctatcgctc cgagatgagc ggtgaggcgc ccgcggagct
     1021 tcgagtttcc gatagcgtct tttcgctcat cattcccacg ctttgggtct tcgggggtgg
     1081 gctcctggcc gaggaaatcg cgggcggttt ctggtcaatc ccggccgccg tggtcgtcgc
     1141 tgccggattt ggcatcatcg ctcatgccga gctcttcagc cttcacgagg agtcttcctg
     1201 gtaccaggcg gctcgcttcg ccttgaacgt cgcggcatac gtcatcgcct tcgcgctgtt
     1261 cgccacgccc tacgcgctcg agctcgggcg attcgccgcg gcgatcttcg tcggcgtggc
     1321 ggcagtgctg atggccatcg acctgctgcg ggaagcgccg atcccggccg tccgcgtcct
     1381 tctcgtggcc ggggccgcgg gcctgctcct cgcagaaacg cgatggtcga tgtactacct
     1441 cggcatcgag gagttctcga gcgcgacgct gctgctcgtt gtgttctatg tggcctcagg
     1501 cttgctccaa tcccaactgc tcgcgaagct ctcggctgcg actgcgctcg agtacgcgct
     1561 tgtcggaggc gcggggcttc tcatcatcgc ggcggccgag ctcatcggat gacgtcccgc
     1621 gtcctcggca tcgatcttgc cagccggggg cgcccgctcg ggtacgccgt cctcgacttc
     1681 ggagcacgga tcatcgcggt ggggcgagat gcgctcgatg atgacgctct gctgcggctc
     1741 gccacgcacc accgtgcctg tcgcgtcgcc atcgacgcgc cgctgttcct tcccgaaggg
     1801 ctctgctgct tggacgatgc gtgcccttgc gctccgctgc aggcgagcgg tctgcgcgcg
     1861 gccgaggtcg ccctgcgcgc acttggcatg ggtggcttcg ccacaacgaa gcggaccatc
     1921 atccctcggc tggccgaacg cgggattggg cttcggcgca gcttcgaggc cgccgggttc
     1981 gacaccgccg aggtctatcc acacggcgcg aaagtccgcc ttttcggaga gcgccgtcca
     2041 cccaagaagg gacgaccggc cggcgacgaa tggctccggc aacgcatcgc ccgcatcgtg
     2101 cgagcgcttc cggaccggct cctccatcac gacgaggccg acgccattct cgccgcgtac
     2161 acaggcctcc tcatcgagcg cggcgaggca gaaccgctgg gcgagagccg cgatgggctc
     2221 gtgtggctgc cggcgggccc acggcttgcg gtcgccagcc gcaccgcgta ggctcggatc
     2281 gtgccactcg agtacgacga cctgccgccg ctggcccgca cgtacctcgc cgtcgtgtcg
     2341 ctaggccttc cttcggcccg gctcgccaac gacgagtcat tccgcatcga ctatgtctgc
     2401 gccgcgtgcc acgcgctctg ggccggccaa gcgatgcgct accacctcga aggcgactac
     2461 caaccgcaga ggggcttcct cgacgagctc cgcgcgacga tcgtcgacct cgacaatgca
     2521 ggcgtcatca tcgcgggcgc cggtgacgga caccttgcga cgatgccgtt cctcggccaa
     2581 gacgcaggca agcttacgcc cggccaaatc gagcggctcg atctgaacgc cccaccgaag
     2641 gtcctcgaca cgtacctggc ttacaaggcg ctcgacgagc tgctgtcgca cccgaaggcg
     2701 tacacgttcg tcatggagaa gtacggcgcg agcagcgaga tctggcagca gctgaccgcg
     2761 cggggatacg gacgttgact atcacgttcg gtgtcctccc ggctcacgac gaatcgggcg
     2821 atctgctggg gagcgtcgag ctcgcggagg cgctcggctt cgattccgtc tggctcggcg
     2881 atcacgtcct atggcacaca cccagccccg acccgatggt cctcctcggc gcgattgccg
     2941 cgcgcacgtc gcgtatcggc atcggcacgg gcatctatct cgctgcgctg cgccccgccg
     3001 cggttgtggc gaagcaggca gccacgctgg accatctggc cgagggacgg ttcatcctcg
     3061 gggtaggcat cggcggcgag aaccccgccg agttcgccgc ggtcggtgtg ccgctcagcg
     3121 agcgggcgtc tcggctggaa gagacgattc gcgcttgccg tgcgctctgg tccgcggatg
     3181 ggcagccggt cgacttcgat ggacggtacg tggcgttccg ccaggtgcgg ttcgattatc
     3241 caccccgcac ccagggcggg ccgcccatat ggatgggcgg gcgggcggag ggagcgttgc
     3301 gccgcgcggg cacgatggcc gatggttggc tcgccttcgt ggtggaccct caacgctatg
     3361 ccgagaattg gcgcaaggtc cgggcgtacg cgacatctgc gggacgggac ccggaccagc
     3421 tcacaccggc gctgcagctt tggtgccagc tcggcgagga tgatagcagt gctcgggcga
     3481 ttctggcgcc cgccatcgag cgcatgtacc ggacgcccta cgagcggttc gaacgctact
     3541 gcattgccgg cgacgcgcag acgtggatac gccgcctggg ggagttcatc gaggcaggcg
     3601 tgcggcactt caatctcatc ttcgcgggtg gcgacgtccg gatgcagatg gttctgttca
     3661 tggatggcgt gcggccgatg ctacggcccg tggactagcg cctttcgcct aacgcgcggt
     3721 agagtatggt caccgtgtaa accgacggta atcacagggc aagaaaggga aatagtgatg
     3781 cgcgagggca actactggac cgaggtgttt cgccggagga tgacccggcg gcgtgcgctg
     3841 ctcgccgcag gcagtggcgc ggctggagcc ggagcgattg gcctcgttgg gtgcggcgac
     3901 gacgacgacg atgacgatga cgaggaggcc ggcaccgcgc ccacaggagg gaccgcggct
     3961 gcgggcgaca tccgtcgcgg aggcacgtac gtcgcgtcgg tcccgatcga cccgccgacc
     4021 ctcgacccgt acaagaacct cagctacctc acccagaccg tggcaggcta cgcttatagc
     4081 cggctgctca agttccagac tggacccgac gttctgccgt ccgactatct tcccgagccg
     4141 gacctcgccg aggcgctccc agaatccccg gatgccatca cctggacgtt caagattcgc
     4201 cccgaagctg tgtatgctcc gggcgaccca ctgcaggggc gcgcggtcac ggccgaggac
     4261 gtgcgctaca gctacgaacg cttcatcaac acgcccgaga atgagcgcaa gaacgacgtc
     4321 atccccgtca tcgaccgcgt cgaggtaatc gacgacaaga cggtgcagtt cacgctcaag
     4381 caagcctatg ccgacttcat cacgctcatg gcctcgtcgt cccacttgtg gatcgttccg
     4441 ccggaggtcg tcgaatcggg actcgtcgaa tcgaagccca tcggctccgg gccgttcatg
     4501 ctcgagagct acgagcggtc gcgccagatg acgttcgcga agaaccccgg ctattgggtg
     4561 atgggcgacg acggccagcc cctaccctac attgatcgaa accagtatct ggtgatcgcc
     4621 gacgcgcaga cgaccgttgc gcagttcacg tcgggccagc tgcatggact cacgatcact
     4681 gaggaacagc gcggacagat cgagagcgac gtgtccggcg tcctcatcta cgatttcctc
     4741 gccaatctcc tcaatcagtt ctggttcgat cctcgtacct actccgagga cctacccccg
     4801 ttcaacgacg agcgagtccg tcaagcgatc tccatgtccg tggaccgcgc gggcctcatt
     4861 cgcctcatct tcaatggcca gggggagtgg cacaaccagg gcgttcccgc gggcctttcc
     4921 tactggtggc tcgacccgca gggcgagaac atcggcgaag gcggccagta ctaccagtac
     4981 aaccccgagg aggcccggaa gcttctcgag gccgccggcg ttcccgacct caaggttcgc
     5041 gtgcactaca ccgaggacgg gtaccgctcc atcgccggtg gcttttacaa ccgtatcgcc
     5101 gattcgctgc ccggcatgtt ccgtgagtcc ggcatcgagg tggagctggt tccggagtcg
     5161 tacagcgccg tctatgtgcc gcagaccttg gctgggaact tcgactccat ggcatggagc
     5221 ctctcgacgc ccttcaactt catccacctc tacctcgtga actactacac aggcacggcc
     5281 ggccgcgacc ggggagtccg cgaccaagaa atcctcgacc tcatcgaccg gcagattcag
     5341 acggtcgacc aggaagagcg ccgcgagctc atctacgaga ttcagcgcat cgcatctgtg
     5401 aagcagtact tccccggcgg ggtgcttgga ccatcgttcc aggccgtgca gccgcagatc
     5461 aagaactact tcgtgacggg cagctacggc accggtacgg agactatcgc gaagctgtgg
     5521 atcgacgcct gagcggtcga cccgaagtac cgagggggct gggaatccag ccccctcggt
     5581 atatgctcac aacacgctgc ctcgcctcac ctcatagcca tcgtcgccgg cgaaagaaga
     5641 agacgagcgc gagcgccgct gcgaccatca caccaagagc gaacggatac cccagcgccc
     5701 agtcgagctc cggcatgttg tcgaagttca tcccgtagat ccccgtgacg agcgacatcg
     5761 tcatgaggat cgtcgcggcg atggtcagcg tcttcatcac ctcattgagg ttgttcgaga
     5821 ccgccgtgag gtaggtctcc atgacggacg tgaccatatc tcgttccagg tcgaccgcgt
     5881 ccgccgcccg cgacacgtgg tcgaacacat cgtcgaagta gcggatggtt tcctcctgct
     5941 cctcgaccag cgggtcgcgg cgtaacgcga cgatgacgtc gcgctcgggt cccagcacgc
     6001 ggcgtaggcc cagcaatcgc cggcggatct gctggcccct cgtcgcgatc tccgccctgg
     6061 gccgagcgaa cacctgatct tcgagctctt ccacctgctc cgcgatgtca tcgacgaggg
     6121 gaaggacgct atcgaccgcc gtatcgagaa tgacgtggag cagatcgctg ctcgtccggt
     6181 cgcgtccgcg ctcatgtgcg gaccaacggc ggcgggcttc cgctatcgat tccacgggac
     6241 cgtggcggac cgtcacgacg tagtcgctag cccaaaagat attgatctcc tgggcgtcca
     6301 gttcgtctgc accactgaag ccgtagaaga cgaggaatcg atacgtatcg tactgatcga
     6361 gcttgggccg ctgattccgc gggtcgaggg catcctccac cgagagcgga tggaaaccga
     6421 atctccgcgc gatgatgtcc atctcctcgg cggtggggtt ctcgacgtcg agccaaagcg
     6481 ggttctcccc gagcacttcc cccggaagct cggaggtcgc gaccatcacc gcaccctgga
     6541 aacgacatcg tcccgaggca tcattgacgc atcg
//
LOCUS       DATMBB010000013         5258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_78763, whole genome shotgun sequence.
ACCESSION   DATMBB010000013 DATMBB010000000
VERSION     DATMBB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78763"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..136)
                     /locus_tag="VNL92_00290"
     tRNA            complement(62..136)
                     /locus_tag="VNL92_00290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Met,seq:cat)
     gene            complement(267..342)
                     /locus_tag="VNL92_00295"
     tRNA            complement(267..342)
                     /locus_tag="VNL92_00295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Arg,seq:tct)
     gene            complement(460..945)
                     /locus_tag="VNL92_00300"
     CDS             complement(460..945)
                     /locus_tag="VNL92_00300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93186.1"
                     /translation="MIVRRAEPRDLGAVTAIYNHWVRTSAATFDIDAFTEDERRPWFE
                     EHGDDYPLLVAERDGEVVGYACLSRFRTKPAYRHTAETTVYLAEAARGAGLGTALYDA
                     LVAEAQRLRYHVLVAGITVPNEASVRLHRRFGFERIGVMHEVGWKFERWHDVEWFELR
                     L"
     gene            complement(962..1255)
                     /locus_tag="VNL92_00305"
     CDS             complement(962..1255)
                     /locus_tag="VNL92_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93187.1"
                     /translation="MAVAIFMKWDGVTAERYDEVRRLVNWEGDVPPGGMLHMAAFDND
                     GMRVTDVWESAEAFQTFVDGRLTPGVQQLGITTQPDVQILPVHALFTPAFKPI"
     gene            1648..2730
                     /locus_tag="VNL92_00310"
     CDS             1648..2730
                     /locus_tag="VNL92_00310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018726287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWO93188.1"
                     /translation="MPMAGVRKIAVLRANGIGDFVFALPALESLRCAYPESEITLLGA
                     EWHRDFLAGRPGPIDRVVVVPRVPGVRGTPDDSDDDATVSAFFEEMRAEQFDLAVQLH
                     GGGRNSNPLILRLGARLTVGMQTPDAPPLDRSLPYCYWQPEVSRYLEVVRLAAARPVA
                     IEPSIAVTQRDLDESMEVVPSDERKIVVLHPGASDPRRRWPASKFGYVASRIAQLGAR
                     VMIVGGDSEIDLAEAVVASSNVPVQSLAGSLTLQQLTGLLARSALVIGNDSGPVHLAE
                     ALGTPTVGIYWCGNVVNGGPMMREWHRVAISWRLHCPACGTHTISDTCQHQQSFVADV
                     PVEDVLAPAFELLDRSAAARPRSVCR"
     gene            2750..3103
                     /locus_tag="VNL92_00315"
     CDS             2750..3103
                     /locus_tag="VNL92_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93189.1"
                     /translation="MASSSKTTTDHEVIRAWVEQRKGHPATVTGTGSSEDAGMLRIDF
                     DEPREDERDERLEPISWEQFFEKFEEKKLAFLYQDKLSSGKESRFFKFVSREGADEGG
                     SRGGRRTSTSGSRRR"
     gene            complement(3133..3948)
                     /locus_tag="VNL92_00320"
     CDS             complement(3133..3948)
                     /locus_tag="VNL92_00320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWO93190.1"
                     /translation="MASVTILIPTCRRPAALAVTLTSLISQTFKDFDIVVSDQTDDAN
                     PYEAGEVRAAARVLEAHGHRVSLLRHLPRRGIAEHRQYLLDRAGGPLALYLDDDLILE
                     PTVVERLVRVICEQECGFVGMAPVGLSYRDDVRPHEQVLEPWDGRVEPETVMPGTVEW
                     QRYRLHNAANCYHVQRRLGLGNDDLLVYKVAWVGGCTLYDVWKLRSVGGFGFWMELPQ
                     EHCGEDVLAQLLVARKYGGCGVLPSGVYHQELETTVPDRRINAPVYLSSLLSP"
     gene            complement(3936..5048)
                     /gene="waaF"
                     /locus_tag="VNL92_00325"
     CDS             complement(3936..5048)
                     /gene="waaF"
                     /locus_tag="VNL92_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWO93191.1"
                     /translation="MDENPLASWEKARHILCVRLDAAGDVLMTSPAIRAVRESAPERR
                     VTLLTSSAGAEAAKLLASVDEVIPYQAPWVKGTTGGVESDLRMLASLRRRCFDAAIIF
                     TVYSQNPLPAALLTYLAGVPLRLARCRENPYELLTTWLPEEEPAQFIRHEVQRQLDLV
                     AAVGCATADDHIRLDVPCASKMQAQRSLAEVGIDGERPWIVVHPGASAPSRRYPPEAF
                     AHVVESLVAEGLQVALTGSPAEVELVETVRSNVRDKTVSFAGRLGLSQLAALIAEAPL
                     LLTNNTGPAHIAAGVATPVVVLYALTNPQHQPWRVPCKVLARDVPCKYCYKSVCPEGH
                     HACLRGVHPEEVVSAVLELLDDTIGLRNRREPAWQV"
     gene            complement(5048..>5258)
                     /locus_tag="VNL92_00330"
     CDS             complement(5048..>5258)
                     /locus_tag="VNL92_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWO93192.1"
                     /translation="IVPFGEDSPVALIEAARPDVFVKGGDYTLATLPEAPLVSALGGT
                     IEILPYVEDLSTTRIIGRILAGAEK"
BASE COUNT          943 a         1746 c         1677 g          892 t
ORIGIN      
        1 tgctaagggc ccttagcaga gtttgcctcc gccaagggcc ctcgccagat tctaagaatg
       61 ctggtagcag gggagggatt cgaacccccg accaagggct tatgagtccc ctgctctacc
      121 gctgagctac cctgccccgt caatcagtat agcatcggtg cctacacggt atcgacggct
      181 ccgcaatgag agcgcgtccg attccgccgt tctccgcccc gcgatcgccg gcgcaatgca
      241 cctcaagccg cccctccgcg gcgctgtggc gcccccggca ggactcgaac ctgcgacccg
      301 cggtttagaa gaccgctgct ctatcctctg agctacgggg gcgatgaatc tgcactcgac
      361 cggttacagc ttatgtccct gagcacctgc aagcaacgga gcatgcaacg cgcttcagcc
      421 gaccgcatcg gccggttccg ctgggcgacg aacagcctgt cagagccgca actcgaacca
      481 ctcgacatcg tgccagcgct cgaacttcca ccccacctcg tgcatgacgc cgatcctctc
      541 gaaaccgaag cgccgatgca gccgtaccga agcctcgttg ggcaccgtga tgccggccac
      601 gagcacgtga tagcggaggc gctgcgcttc ggccacgagc gcatcgtaga gcgccgtgcc
      661 gagcccggcg ccgcgcgccg cctcggccag atagaccgtc gtctccgcgg tgtggcgata
      721 ggccggcttg gtccggaagc gcgacaggca tgcgtagccc accacctcgc cgtcgcgctc
      781 ggcgacgagg agcggatagt cgtcgccgtg ctcctcgaac cagggccgcc gctcatcctc
      841 ggtgaacgcg tcgatatcga aggtcgccgc actcgtccgg acccagtggt tgtagatggc
      901 cgtgacggcg ccaaggtcgc ggggctcggc acggcgcacg atcatggcgg cacgcgactc
      961 gctagatggg cttaaaggcc ggcgtgaaca gcgcgtggac gggcagaatc tgcacgtcgg
     1021 gctgcgtcgt gatgcccagc tgctgaacgc cgggcgtgag ccgaccgtcc acgaaggtct
     1081 ggaacgcctc cgcgctctcc catacgtcag tgacgcgcat tccgtcattg tcgaaggcgg
     1141 ccatgtggag cataccgccg ggaggaacgt cgccctccca gttcacgagc ctgcgcactt
     1201 cgtcataccg ctccgcggtc accccatccc acttcatgaa gatagcgaca gccatgtagt
     1261 gcccccattt tggttttggg ggcgagccta cccaatgctc acgcccgtcg tcaaccggcg
     1321 ggcggtcggt gccgggcgga gagcaacgcc agggctgggc cggggttctg ggctatgacc
     1381 tattgcacaa ggtagagcgt ggggtcgaag aacacccaca ccacgtcgac gaagtgccag
     1441 tagctcccca cccgctgcgc cgacaactga gacccggcgc gcaacgcctt tgcaacgtcg
     1501 ggacacccag ggttggtcgt gctgcaactc gctcgcccta gaatgatctt cgatgcgtga
     1561 ccgtcacggc agccgccaag gcacgtggat tcgagggtga tgttcggcgt ggccgatatg
     1621 cggagtgacg tgcgatccga tacgccgatg ccaatggcgg gagtccggaa gatcgccgtt
     1681 ctccgcgcca acggcatcgg cgacttcgtg ttcgccctcc cggcgctcga gtcgttacgc
     1741 tgcgcctatc ccgaaagtga gatcacgctg ctcggcgctg aatggcaccg cgatttcctc
     1801 gccgggcggc cgggcccaat cgaccgcgtt gttgtcgtgc cgcgcgttcc cggtgtgcga
     1861 ggcaccccgg atgatagcga tgatgacgca acggtctcgg ccttcttcga ggagatgcgc
     1921 gccgagcagt tcgacctcgc cgtccaactc cacggcggtg gccgcaattc gaacccgctt
     1981 atcctccggt tgggcgctcg ccttaccgtg ggaatgcaga cgcccgatgc tccgcccctc
     2041 gatcggtcgc tgccgtattg ctactggcag ccggaggtat cccgatacct cgaggtcgtg
     2101 cgactggccg ccgcgcggcc tgtggcgatc gagccatcga tcgccgttac ccaacgcgac
     2161 ctcgacgagt cgatggaggt cgtgccgagt gacgagcgga agatcgtcgt cctccaccca
     2221 ggcgcgtccg atccgcgacg ccgctggccg gcctcgaagt tcggctacgt cgcgtcccgc
     2281 atcgcgcagc tcggcgcgcg cgtcatgatc gtcggcgggg attcggagat cgatctggcc
     2341 gaagcggtcg tcgcgtcgag caacgtgccg gtgcagtcgc tcgctggcag cctgacgctt
     2401 cagcaactga ccggtctcct cgcgcgttcg gcactcgtca ttggcaacga ttctgggccc
     2461 gtccatctgg ccgaagcgct cggaacgccc accgtcggca tctactggtg cggcaacgtc
     2521 gtgaacggcg gcccgatgat gcgcgagtgg catcgcgtcg cgatttcctg gagactccac
     2581 tgccccgctt gcggcaccca caccatcagc gatacatgtc agcaccagca gtcgttcgtg
     2641 gccgacgtac ccgtggagga cgtgctggca ccggcgtttg agctcttgga tcgatcagcg
     2701 gcagcacggc ccaggtcggt ctgccgttag ccaagaagga ggtctgatca tggctagctc
     2761 gagcaagacg accacggacc atgaggtcat tcgcgcgtgg gtcgaacaac gcaagggcca
     2821 tccggccacc gtcaccggga ctgggtcgag cgaggacgca ggcatgctcc ggatcgactt
     2881 cgacgagccc cgcgaggacg agcgagacga gcgtttggag ccgatctcct gggagcagtt
     2941 cttcgagaag ttcgaggaaa agaagctcgc cttcctttac caggacaagc tctcctcggg
     3001 caaggaaagc cggttcttca agttcgtttc gcgagaaggt gccgacgagg gcggcagccg
     3061 gggaggccgg cgcacctcta cgagcgggag ccggcgacgt tagagtcagc gcgaactgtg
     3121 agctcgaaga agctaggggc tcagcaggga cgataggtag accggagcat tgatacgccg
     3181 gtcaggcacg gttgtttcca actcctggtg gtagacgccg ctggggagga cgccgcagcc
     3241 cccatacttc ctcgcaacga gcaactgcgc cagcacgtcc tcgccacagt gctcctgagg
     3301 caactccatc cagaagccga agcctcccac gctgcggagc ttccacacgt cgtagagggt
     3361 gcagcccccc acccaggcga ctttgtacac gagcaggtcg tcgtttccca ggccaaggcg
     3421 gcgctgcaca tggtagcagt ttgccgcgtt gtgcaagcga tagcgctgcc actccacggt
     3481 tccaggcatg acggtctccg gctccacacg gccatcccac ggctccaaca cttgctcgtg
     3541 ggggcgcaca tcatcacggt acgaaaggcc aacgggtgcc atgccgacga atccacactc
     3601 ctgctcgcag atgacgcgga caaggcgctc gacgaccgtc ggctccagga tgaggtcgtc
     3661 gtcgagatac agcgcaagcg gcccgcccgc cctatcgagc agatactggc ggtgttcggc
     3721 tataccgcgc cgcggaagat gtcgcaggag cgacacacgg tggccgtgag cctcgagaac
     3781 acgcgcggcg gcgcgtacct cgccggcttc gtagggattc gcgtcgtccg tctgatcgga
     3841 cacgacgata tcgaagtcct tgaaggtttg gctgataagg ctggtcagcg tgacggccag
     3901 ggccgcgggc cggcggcacg tcgggatgag gatcgtcaca cttgccatgc aggctccctc
     3961 cgattgcgaa gcccgatcgt atcgtccagt agctcgagga cggcgctcac cacctcctcc
     4021 ggatggaccc cgcgcaaaca ggcgtgatgc ccctcgggac agacgctctt gtagcagtac
     4081 ttgcagggca cgtcgcgagc aagcaccttg cacggcacac gccatggctg atgctgcgga
     4141 ttcgtcagcg catagagcac gacgacagga gtagcgacgc cggctgcaat gtgcgcagga
     4201 ccggtgttgt tcgtaagaag gagcggcgct tcggcgatca acgctgccag ttggctgagg
     4261 cctagcctcc cggcgaagct gacggtcttg tcccgcacgt tcgatcgtac cgtctcgacc
     4321 agctcgacct cggcggggga cccggtgagc gcgacctgaa ggccttccgc cacaagcgac
     4381 tcgaccacat gagcgaaggc ttcgggggga tagcgacgcg aaggagcgct cgcgcctggg
     4441 tggacaacga tccacggacg ctcgccatcg attcctactt ctgccaggga cctttgagcc
     4501 tgcattttgc tagcacaggg cacgtcgagg cgaatgtgat cgtccgcggt cgcgcaaccg
     4561 accgcggcga cgagatcgag ttggcgctgc acctcatgcc ggatgaactg cgccggctct
     4621 tcctcaggca gccaggtcgt cagcaactca tatggattct cgcggcatcg cgccaagcgt
     4681 agcggtacgc ccgcgagata ggtcagcagc gccgccggta gggggttctg gctgtacacc
     4741 gtgaatatga tggcggcatc aaagcagcgc cgccgcaagc tcgccagcat ccgcaggtcc
     4801 gactccacgc cgccggtcgt gcccttcacc cacggagctt ggtagggaat gacctcgtcg
     4861 acgctggcca ggagcttcgc tgcctccgcc cccgcacttg aagtcagcag ggtcaccctg
     4921 cgttcggggg cgctctccct caccgcccgg atggcgggcg aagtcatgag cacgtcacct
     4981 gccgcgtcga gcctgacgca caggatgtgt ctcgcctttt cccacgatgc cagagggttc
     5041 tcgtccatca cttctcggcg ccggcaagga tgcggccgat gatgcgtgtc gtcgagaggt
     5101 cctcgacgta cgggaggatc tctatggttc caccgagggc gctcacaagc ggcgcctccg
     5161 gcaaagttgc gagcgtgtaa tcgcctccct tgacgaacac atccggccga gccgcttcga
     5221 tgagcgcaac cggcgagtct tcgccgaagg gcacgatg
//
LOCUS       DATMBB010000014         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_231166, whole genome shotgun sequence.
ACCESSION   DATMBB010000014 DATMBB010000000
VERSION     DATMBB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231166"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2077
                     /locus_tag="VNL92_00335"
     CDS             <1..>2077
                     /locus_tag="VNL92_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93193.1"
                     /translation="RVPRLHRRHLRRPRLTQLLDRSPAQVLLVTAPAGFGKTSLACEW
                     LEGRTDVAWYRATSASADLAAFSVGISEAIAPIVPGAGDRLRQRLLVAEPPENAARPM
                     AELLAEDLAEWPLGAWLVLDDYHLVTGSSPVEEFMDWLLTLAPDLRVLVTARRRPAWA
                     SARRFLHGEVFELTARQLAMSDAEAAKVLGGRSGETVRRLVAQAQGWPALIALAGLSA
                     SAEVPEELVTERLYRYFAEEVLRTQTPQLQRFMLVSSVPSSIGTELGRALGLEDVEST
                     IACLLDDGLLHQAEAGEAAFHPLLRDFLRRKLESDQPEQFAELADRAVAFGRRNARWE
                     EAFDLAVYRAQLDTAAQILAEAAEDLLPAGRIETLEHWLDACGPAAATTPALLVKAEI
                     LLRRAKPLDAHGLAQAIADSLDPASPHLSRAWSLAGQTAHFLSQPEVALSCHRAALAT
                     ATSDAEAATALWGATLAAAELELDEAVDYLQQFERSSRNNLGARLRMAQGRSMVARYS
                     GGFADSWALLRSTLPLVDHANDPHAESSFLAYMSYVSSLRGDYRLGAQLGERAQLMCE
                     RYRWAVGVLVSQVVRAYAELGVRNLRAVRAILNDIRKTDLDNLDPYVETSTLIIGTRL
                     AVAEGDLERALACASRTTRGKPTESIAAELLASRAIVEAATGQAPVAASHCLEALRRS
                     RCIEVKHLAA"
BASE COUNT          332 a          744 c          633 g          368 t
ORIGIN      
        1 acgcgttccg cggctccatc gccgccacct gcgacggccg cggctcactc agctactcga
       61 ccgcagcccg gcgcaggtgc tcctcgtcac cgctcctgcg ggcttcggga agacctccct
      121 cgcatgtgaa tggctcgagg gccgaacgga tgtcgcctgg taccgcgcga cctcggcctc
      181 ggccgacctc gccgcgttct ccgtgggcat ctccgaggcg atcgccccga tcgtccccgg
      241 cgccggcgac cgccttcgac agcggctgct cgtggccgag ccacccgaga acgcggcccg
      301 gccgatggcg gagctcctcg ccgaggatct cgccgagtgg ccgctcggcg cctggctcgt
      361 cctcgacgac taccacctgg tgaccggctc gagcccggtc gaggagttca tggactggct
      421 cctgacgctc gcccctgacc tgcgcgtgct cgtcaccgcc cgacggcgcc ctgcctgggc
      481 gtcggcgcgc aggttcctgc acggggaggt cttcgagctc accgcgcgcc agctcgcgat
      541 gagcgacgcg gaagcggcaa aggtgctcgg cgggaggtcg ggcgagacgg tccgccgcct
      601 cgtggctcag gcccagggct ggccggccct catcgccctc gcgggcctgt ccgcctctgc
      661 cgaggtcccc gaggagctcg tcacggaacg cctctaccgc tacttcgccg aggaggtgct
      721 gcgcacgcag acgccgcagc tccagcgctt catgctggtc tcctccgtgc cgtcctcgat
      781 cggcaccgag ctcggccgcg cgctcgggct cgaggacgtc gagtcgacga tcgcctgcct
      841 gctcgacgac ggactcctcc accaggcaga ggccggagaa gccgcgtttc atccgctgct
      901 gcgcgacttc ctgcgccgga agctcgagtc ggaccagcca gagcagttcg ccgagctggc
      961 cgaccgcgcc gtcgccttcg gccgccgcaa cgcccgctgg gaggaagcct tcgacctcgc
     1021 cgtctaccgg gcccagctcg acacggcagc acagatcctc gccgaagcag ccgaggacct
     1081 cctgccagcg ggccgcatcg aaacgctaga acactggctg gatgcctgcg gccccgccgc
     1141 cgcaacgacc cccgcgcttc ttgtcaaagc tgagatcctc ctgcgacgag ccaagccgct
     1201 ggatgctcac ggcttggcgc aggcgatcgc agacagtctc gaccctgctt cacctcacct
     1261 ctcgcgggca tggagtctcg cgggacagac cgcacatttc ctttcacaac ccgaagtagc
     1321 gttgtcttgc catcgagcag ccctcgccac agcgacctcc gacgccgagg cagcaactgc
     1381 attgtggggc gcaacactcg cagccgccga gcttgagttg gatgaagcgg tagattacct
     1441 tcaacaattc gagcggtcgt cgcgtaataa ccttggcgcg cgcttacgaa tggcccaggg
     1501 tcgttcgatg gtcgccagat atagcggtgg attcgcagat tcctgggcgc ttctccgatc
     1561 gactctgcct ctcgttgacc acgccaacga tccacacgcc gagagctcct ttctcgcgta
     1621 catgtcgtac gtcagctctc ttcgcggtga ctatcgtctc ggtgctcaac tcggagagcg
     1681 agcgcaactg atgtgcgagc gctatcgctg ggctgtcggc gtgcttgtct cccaggtggt
     1741 ccgcgcatac gcggaactcg gtgttcgtaa cctgcgtgcc gtccgtgcca ttctaaatga
     1801 catacgaaag actgacctcg ataatctcga tccgtatgta gagacttcga cactcatcat
     1861 cggtactcgc ttggcggttg ccgaaggtga cttggagcgg gcacttgcct gcgcttcccg
     1921 aactacccgt ggcaagccaa cagagagtat cgccgctgaa ctccttgctt cccgggcaat
     1981 cgtagaggca gcaaccggtc aagcccctgt cgctgcgtct cattgcctcg aagccttacg
     2041 aagaagtcga tgtatcgagg taaagcatct cgctgca
//
LOCUS       DATMBB010000015         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_78159, whole genome shotgun sequence.
ACCESSION   DATMBB010000015 DATMBB010000000
VERSION     DATMBB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78159"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VNL92_00340"
     CDS             complement(<1..262)
                     /locus_tag="VNL92_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93194.1"
                     /translation="MTTDASSPRAPNPIDTWRQFITDSERQWNSFFKEVLGTEGFSSA
                     MNTWVEASLTVQRMVADQLERLYTTFNIPTHNDLVALGERMKA"
     gene            complement(259..687)
                     /locus_tag="VNL92_00345"
     CDS             complement(259..687)
                     /locus_tag="VNL92_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWO93195.1"
                     /translation="MPTKKGPNATEQPFRLHGDLLTTTLLAFLRNWNAYGYQLAQQLA
                     EAGLPAFDSGTVYRTLRQLERSGLVSSFWDTSESGPARRMYSLTRAGEIFLSSWIDVL
                     GKYQAVLQRTIEGMERDGLGEDADPQRAAGDRARQQGEQP"
     gene            821..1333
                     /locus_tag="VNL92_00350"
     CDS             821..1333
                     /locus_tag="VNL92_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93196.1"
                     /translation="MAQTTEQTQSGPVDKYFEALTESYDAIIEAIKAGNERGFRISNN
                     LLAEAQRGQREAVELGKKLAENPTDIGGFYRAVMESVTRAQGRTLELARQMFDEMSES
                     RKEGREVIEKVIKAQRAAGEAAIEATRDVASTAAERVRTGVSRVTTRATEATEQAAGT
                     ARKAAASAAE"
     gene            1458..1533
                     /locus_tag="VNL92_00355"
     tRNA            1458..1533
                     /locus_tag="VNL92_00355"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1491..1493,aa:Ala,seq:cgc)
     gene            1790..>2394
                     /locus_tag="VNL92_00360"
     CDS             1790..>2394
                     /locus_tag="VNL92_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWO93197.1"
                     /translation="MATHPLVLLADDEPGILRLLQVEMAAQGFDVITASDGDEAVRLA
                     DERRPDIAVLDIIMPRMNGLEAMRRMHETRPIPVILLTARDSEADKVRGLELGADDYL
                     GKPFSPEELTARVRAILRRTSAGKTEEHILRHGNVEIDLTRRLVRKDGQIVQLSRTEW
                     MLLQQLASNPGKIMLNVELLHKVWGAEYCDDLQYLRVWISR"
BASE COUNT          428 a          831 c          760 g          375 t
ORIGIN      
        1 tcgccttcat ccgctccccg agtgccacca ggtcgttgtg cgtagggata ttgaacgtcg
       61 tgtacagccg ctcgagctgg tccgcgacca tccgctgcac cgtgagcgac gcctcgaccc
      121 aggtgttcat cgcgctcgag aagccctccg tcccgaggac ctccttgaag aagctgttcc
      181 attgccgttc ggagtcggtg atgaactgcc gccaggtgtc gatggggttc ggcgctcggg
      241 ggctcgacgc atctgttgtc atggctgctc tccttgctgg cgggctcggt cgccggccgc
      301 acgctgcggg tcggcgtctt cccccaaccc gtcacgctcc atgccctcga tcgtccgctg
      361 gagcaccgcc tggtacttcc ccaagacgtc gatccagctc gaaagaaaga tctcgcccgc
      421 gcgggtcagt gagtacatgc gccgcgccgg cccggattcc gacgtgtccc agaacgacga
      481 caccaggccg ctgcgctcga gctgccgtag cgtccggtac accgtgccag aatcgaacgc
      541 gggcagcccc gcctcggcga gctgctgggc gagctggtag ccgtatgcat tccagttccg
      601 caagaacgcc aacagggtgg tcgtcagcag gtcgccgtga agccggaacg gctgctcggt
      661 cgcgtttggt cccttcttcg tcggcacacg gccctcctgc gtcaagatag cggaatgcag
      721 cccttgacag cattccgtcg ctaggtgtaa gttacatcta tctgagcagt gagcgcaagc
      781 ccggagcagc gctcgaccac acgaaaggac ggtttctccc atggcccaga ccaccgaaca
      841 gacgcagagc ggccccgtcg acaagtactt cgaggccctc accgagagct acgacgccat
      901 catcgaggcg atcaaggccg gcaacgagcg tggcttccgc atctcgaaca acctcctcgc
      961 cgaggcgcag cgcgggcagc gcgaggccgt cgagctcggg aagaagctcg ccgagaaccc
     1021 gacggacatc ggcggcttct accgcgccgt gatggagtcc gtcacgcgcg cccagggccg
     1081 cacccttgag cttgcgcggc agatgttcga cgagatgtcc gagagccgca aagaggggcg
     1141 cgaggtcatc gagaaggtga tcaaggcgca gcgcgccgcc ggcgaagcgg ctatcgaggc
     1201 cacgcgcgat gtcgccagca ccgccgccga gcgcgtgcgg accggcgtca gccgcgtcac
     1261 gacgcgcgcc accgaagcga ccgagcaggc ggccggcacg gccaggaagg cagcggcctc
     1321 cgccgcagag taaactgacg ccaacaggcg cccgcgaggc ggcgccccgc tgatgcgggc
     1381 gccgcctctg cacctctggc acaggcatcg tgccactcgt tgagggcccg gtgcgacccc
     1441 gttatactct cggcgacggg gatatagctc aactgggaga gcgccgcgtt cgcaatgcgg
     1501 aggttagggg ttcgagtccc cttatctcca ccagacgccc gtccacgaag ccctcgctcc
     1561 cgcgagggct tctgcagttt gcggacaagg gcatcggcgg cgccaatgtc cgcggccctg
     1621 tggtatgaaa atacatcgac ccgtcttcgc acgtcgcgca acgccacatt gccggtagta
     1681 ggatcaagag gtcctgatgg cgcggacggg acgtccgccc acccttccag gccgcaccaa
     1741 cactcaggcg tcgcgcacct ggcggcctct gcatcgcagg aggtgaagca tggcgaccca
     1801 tccattggtc ctcctcgccg acgacgaacc cggcatcctg cgcctgctcc aggtcgagat
     1861 ggccgcccag gggttcgacg tgatcaccgc atccgacggc gatgaagccg tgcggcttgc
     1921 cgacgagcgg cgcccggaca tcgccgtcct cgacatcatc atgccgcgca tgaacggcct
     1981 cgaggcgatg cgccgcatgc atgagacgcg tcccatcccg gtcatcctcc tcaccgcgcg
     2041 tgacagcgag gccgacaagg tgcgcgggct ggagctcggc gcggacgact atctcggcaa
     2101 gccgttcagc ccggaggaat taacggcgcg tgtgcgggcg atcctccgcc gcacctcggc
     2161 cggcaaaacc gaggagcaca tcctgcgcca cggcaacgtc gagattgacc tcacgcgccg
     2221 gctcgtccgc aaggatggcc agatcgtgca gctctcccgc accgaatgga tgctcctgca
     2281 gcagctcgct tcgaaccccg ggaagatcat gttgaacgtc gaactactgc ataaggtctg
     2341 gggcgccgag tactgcgacg acctccagta cctccgcgtg tggatatcgc gcat
//
LOCUS       DATMBB010000016         2039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_500849, whole genome shotgun sequence.
ACCESSION   DATMBB010000016 DATMBB010000000
VERSION     DATMBB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500849"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VNL92_00365"
     CDS             <1..589
                     /locus_tag="VNL92_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HWO93198.1"
                     /translation="SFCDGGKFVVTLGGEHTVSVGAVRAHAARIPHLSVLVFDAHADM
                     RDEYLGTPYNHACSIRRHLERCPVVQVGLRSASAEEAREIRSRDLPFYTPEAFRALPD
                     GPRTIVEHLSSNVYVNIDIDAFDPSQVVGVGTPEPGGLYWPEVDAILRRVASERRIVG
                     FDVTELAPVYGTEADAHLAAKLVYRMIGLAFGPPA"
     gene            605..1843
                     /locus_tag="VNL92_00370"
     CDS             605..1843
                     /locus_tag="VNL92_00370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93199.1"
                     /translation="MAEIRHIRDDEVDERLALSADAFGFSRSSPEAAQFFREHVDLQD
                     TFGAFEEGRLVAALHYRPMETRLNDRWVPMAGVMSVGSRPEVRRGGHVGALLRAALGQ
                     MHERGYAVSTLFPTFYALYRRYGWEVGAADIAYRFDSHDLGDLQIEPYGTFARVQAED
                     WPALNEIYEEATRLRNGALRRDGRWWSEAVFLGRDKQPRDAILWRDESGKASGYVIYS
                     RGGHAMLFEADQSRRQLAGHELTIRELMATGSEAYLQLIRFLQGHDLARRVVWTAPAD
                     DPLLTIAPDPRRILAERRASFMFRIVDVPRALCERGAPRRLRPMGLRLAVHDSRCAWN
                     EGTWQIEVAERGEMSVERVTGPADAEVDIGMLAALVTGHTDVRTARILGRLSLQSEAK
                     ADDIASFFAAAAAPYCSDFF"
     gene            1852..>2039
                     /locus_tag="VNL92_00375"
     CDS             1852..>2039
                     /locus_tag="VNL92_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWO93200.1"
                     /translation="MSPEPATWTLATCAEAMARGEVSSTELTQAFLDRAEALNPVLNA
                     YLLIDREGALAAARRADE"
BASE COUNT          326 a          709 c          665 g          339 t
ORIGIN      
        1 ctccttctgc gacggcggca agttcgtcgt cacgcttggc ggcgagcaca cggtgagtgt
       61 cggcgcggtg cgcgcccacg ccgcccgcat cccccatctg tccgtcctcg tcttcgatgc
      121 gcacgccgac atgcgcgacg agtacctggg gactccgtac aatcacgctt gcagcatccg
      181 ccggcacctc gaacgctgcc cggtggtgca ggtcggtcta cgcagcgcca gcgccgagga
      241 ggcgcgcgag atccggagcc gggaccttcc gttctacacc cccgaagcct tccgcgccct
      301 gcccgatggg cctcgcacca tcgtcgaaca cctgtcgtcc aacgtgtacg tcaacatcga
      361 catcgacgcg ttcgatccct cgcaggtcgt gggcgtcggt acgcccgagc ccggcggcct
      421 ctattggccg gaagtcgatg ccattctccg ccgcgtcgcc agcgagcgcc gcatcgtcgg
      481 gtttgacgtg accgagctcg cgcccgtgta cggtacggag gccgatgcgc acctcgcagc
      541 gaaactcgta taccggatga tcggcctcgc cttcggccca ccggcatagg gagtggcaag
      601 agccatggcc gaaatccggc acatccgcga tgacgaagtc gacgagcggc tcgccctatc
      661 ggccgatgct ttcggcttct cccggtcctc tccggaggcc gcacagttct ttcgcgaaca
      721 cgtcgatctc caggacacgt tcggggcgtt cgaagaggga cgcctcgtgg cggcccttca
      781 ctatcggccc atggagaccc gcctcaacga tcgttgggtc cccatggccg gcgtgatgag
      841 cgtcggttcc cggccggagg tgcgtcgcgg aggccacgtt ggcgccctgc ttcgagccgc
      901 cctcggccag atgcacgagc gtggctacgc cgtatcgacg ctgttcccaa ccttctacgc
      961 cctctatcga cgatacgggt gggaggtggg cgctgcggat atcgcgtacc gattcgacag
     1021 ccacgacctc ggcgacctgc agatcgagcc gtacggcaca ttcgcacgcg tgcaagccga
     1081 ggactggccg gcgctgaacg aaatctacga ggaggcgacg cggctgcgaa acggggcact
     1141 ccgccgcgat ggacggtggt ggtcggaggc tgtgtttctt gggcgcgaca agcagccacg
     1201 cgacgccatc ctgtggcgcg acgaatcggg aaaggcctcc ggctacgtca tctactcccg
     1261 cgggggccac gcgatgctgt tcgaggccga ccaatctcgc cgccagctgg ccggacacga
     1321 gctgacgatc cgggagctca tggccaccgg gagcgaggcc tatctgcagc tcatccgctt
     1381 cctgcagggc cacgatctcg cgcggcgtgt cgtctggacg gcgcccgccg acgatccgtt
     1441 gctgactatc gcgccggacc cgcgtcgcat cctggccgag cggcgcgcgt cgttcatgtt
     1501 ccgcatcgtc gatgtgccgc gcgcgctttg cgagcgtggc gccccgcggc gcctccgtcc
     1561 catgggactg aggctggcag tccacgattc gcgctgcgcc tggaatgagg gcacgtggca
     1621 gatcgaagtc gcggagcgag gcgagatgtc ggtcgagcgt gtcaccgggc cagccgatgc
     1681 cgaggtcgat ataggcatgc ttgcggcgct cgtcactggc cacaccgacg tacgcactgc
     1741 acgcatactc ggccggctca gcctccagtc cgaagcgaag gcggatgaca tcgcgtcctt
     1801 cttcgccgcc gcagcggccc catactgcag tgatttcttc tgaggagcgc catgtcaccc
     1861 gaaccagcca cgtggaccct cgccacatgc gcggaagcga tggcacgtgg cgaggtcagc
     1921 tcgactgagc tcacgcaggc gttcctcgat cgggcggagg cgctcaaccc ggtactgaac
     1981 gcctatctcc tcatcgatcg cgagggggcg ctcgcggcag cacgcagggc cgacgaaga
//
LOCUS       DATMBB010000017         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_77467, whole genome shotgun sequence.
ACCESSION   DATMBB010000017 DATMBB010000000
VERSION     DATMBB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77467"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1401)
                     /locus_tag="VNL92_00380"
     CDS             complement(70..1401)
                     /locus_tag="VNL92_00380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWO93201.1"
                     /translation="MAGASGLALFGGSPVRRDPLPLYNMIGEREKQAVMEVLNGGVLS
                     GFAAQPNRDFFGGRWIEALEADYCARFVVRHAVAVNSATSGLHAALAATGIGPGDEVI
                     VPPYTMSASATTVLYTGAVPIFADIDDEIFCLDPDAVEANITPYTKGIMAVNLFGHPA
                     PLLRLREIADRHGLFLIEDNAQAPGATVDGRYAGTVGHLGIFSLNRHKAMQCGEGGVV
                     ICNDDDLALRVRLVRNHGEVLVEAMKIGNIVNTAGLNYRMTNMEAAVARVQLSRLDEI
                     NAPRVRLAKRLREGLSTLAGIKPASVKEGSTHVYYFFAMTYDEKATGIPRKLFARAVE
                     AEGFTLKAGYVKPIYLEPLYQRRICFGKDGFPFSANPRNRQISYSKGICPVCERLQDH
                     ELMWTNIIYPPLTETDMDAFVEACRKVLSRRDELLAGARTLSRSGGDTLYD"
     gene            complement(1419..>2250)
                     /locus_tag="VNL92_00385"
     CDS             complement(1419..>2250)
                     /locus_tag="VNL92_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWO93202.1"
                     /translation="ATGRIEDLPPGIAQAAVVATPPAGRATTIRQLLDGGCAIIVVEK
                     PLADSYDEGRRIVDACAAAGATLRVNFNRRFDPAYRRFRARLPGRPLALVAVYGKGLR
                     NYASHMIDLVMEWFGPVVAAQALCGQPLGDGDPSIAFRVALRDGPDLIATGIDGIAYD
                     QFELDLYFRDRRFQLANGGAEKRCSTGVSGWLYPSYVHLAPGQLIEPVTPVGGYRELH
                     EAIWQHLSVGTPMPGCTGPEALTNLLVELALRRSAADGGRAVSLLELAASAPFSGTIT
                     "
BASE COUNT          423 a          735 c          725 g          367 t
ORIGIN      
        1 gccgccacag cggcacgagg taggcggcgg aacctggctg gccgacgacg aactctatga
       61 tggccggcat cagtcgtata gcgtgtcacc gccgcttcgg ctcagcgtgc gtgcgccggc
      121 caagagctca tcccggcgcg agaggacctt ccggcacgcc tcgacgaagg cgtccatatc
      181 cgtctccgtg agtggcggat agatgatgtt cgtccacatg agctcgtggt cctgaagacg
      241 ctcgcaaacc gggcagattc ccttcgagta cgagatttga cggttgcggg gattagcgga
      301 aaacggaaaa ccgtccttac cgaagcagat gcgcctctga tagagaggct cgagatagat
      361 cggcttgacg taaccggcct tgagggtaaa gccttccgcc tccaccgcgc gggcgaacag
      421 cttgcgcgga attccggttg ctttctcatc ataggtcatt gcaaagaagt agtagacgtg
      481 cgtcgagccc tccttgacgc ttgcgggctt gatgcccgcg agggtcgaca gcccctcacg
      541 cagccgcttc gcgagcctga cacgcggcgc attgatctca tcgagacgcg agagctgcac
      601 gcgcgcgacc gccgcctcca tgttcgtcat tcgatagttc aggcctgccg tattgacgat
      661 attcccgatc ttcatggcct cgacgaggac ctcgccgtgg ttccgcacca agcgcacccg
      721 cagggcgagg tcgtcatcat tgcagatgac cacaccgcct tcgccgcact gcatggcctt
      781 gtgccggttg aggctgaaga ttcccaggtg tccgacggtt ccagcgtagc gcccgtcgac
      841 agtcgccccg ggcgcctgcg cgttatcctc gatcaggaac aagccatggc gatcggcgat
      901 ctcgcgcagc cgaagcaaag gggccgggtg tccgaagaga ttcacggcca tgatgccttt
      961 ggtgtagggc gtgatgttcg cctcgacggc atcgggatcc aggcaaaaga tctcgtcatc
     1021 gatatcggcg aaaatgggga cggcgcccgt gtagagcacg gtggtggcac tggccgacat
     1081 ggtgtagggc ggcacgatga cctcgtcacc cggcccgatg cccgtggcgg ccagcgcggc
     1141 gtggaggccc gaggtggcgg agttcacggc gacggcatgg cggacaacga agcgggcgca
     1201 gtagtccgcc tccagcgctt cgatccatcg gcccccaaag aagtcgcgat tcggctgcgc
     1261 cgcgaacccg gagaggacgc cgccgttaag gacctccatg accgcctgct tctcacgctc
     1321 gccgatcatg ttgtaaagcg gcagtggatc ccgacgcacc ggcgaaccac cgaatagggc
     1381 caagccgctc gcacccgcca tcaccagtaa ctcctgcgct acgttattgt cccagagaac
     1441 ggcgccgacg ccgccaattc gagaagcgaa accgcccggc cgccatccgc cgccgaccga
     1501 cggagggcga gctcgaccag caggttggtg agcgcctcag gcccggtgca ccccggcatc
     1561 ggcgtcccga ccgacagatg ctgccagatc gcttcgtgca actcgcgata tccgcccacc
     1621 ggcgtcaccg gttcgattag ctggccgggg gccagatgga cataggacgg atagagccac
     1681 ccgctaacgc ccgtgctgca gcgcttctcg gctccgccat tggcaagctg aaaacgccgg
     1741 tcacggaagt agagatcgag ctcgaactgg tcgtacgcaa taccatcaat gccggtggcg
     1801 atgaggtcgg ggccgtcgcg cagagccacg cgaaatgcga tggaagggtc cccgtccccg
     1861 agcggctgcc cgcacaacgc ctgggccgct accaccggtc caaaccactc catcacgaga
     1921 tcgatcatgt ggctggcgta gttgcgaagg cctttgccgt agacggcgac gagagcgagc
     1981 ggccgccctg gcaggcgagc gcggaatcgg cgatacgccg gatcgaaccg tcggttgaag
     2041 ttgacccgca gggtagcccc ggccgccgcg cacgcgtcca cgatcctgcg cccctcgtcg
     2101 tacgagtcgg caaggggctt ctcgacgacg atgatcgcac acccgccatc gagaagctgt
     2161 ctgatcgtcg tggcgcgccc ggccggcggc gtggcgacga cggcggcctg cgcgatgccg
     2221 ggcggtagat cctcgatgcg gcctgtagcc
//
LOCUS       DATMBB010000018         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_276432, whole genome shotgun sequence.
ACCESSION   DATMBB010000018 DATMBB010000000
VERSION     DATMBB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276432"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VNL92_00390"
     CDS             complement(<1..324)
                     /locus_tag="VNL92_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93203.1"
                     /translation="MGRMLFRRHPFRLSAIVAVLLAGLVLAACNSDGTAESGGTAVAS
                     PIGQEEADRALRGLCEARDLAGREDVEAARNVFFDEAHQFLHELTVPLQERDAAAARD
                     LLVAKN"
     gene            complement(370..1383)
                     /gene="mshD"
                     /locus_tag="VNL92_00395"
     CDS             complement(370..1383)
                     /gene="mshD"
                     /locus_tag="VNL92_00395"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HWO93204.1"
                     /translation="MSLQVRPKVEELYGRRMHHIEFVLKLGKEIQPAVVDLVQAAAEH
                     DGHSPLGEHKFLRLSHGDDTSIGVLAWEEQRLMGYAQSVFFTDENGSRLACELVVHPE
                     ARGQGVGNLLLRHLFDVAERYGAQYMDAWAYMDTPSARALAAAYAFRPARVLLHLRRD
                     IAGDVAEPCQLPSGLRLARFRPGIDDREWLALNARVFSHHPEQGRWTHVDLEARLREA
                     WFDPDDFLVLRDAEGRMIAFNWLKVGRDAERDGAEIYVIGVAPEWAGRGIGTALITLG
                     VQHVAEQGCRHVCVYTDAENGPALRLYDRMGFTGRHRDVCYRRLLEASPSPDSDGGRA
                     AVG"
     gene            1540..>2176
                     /locus_tag="VNL92_00400"
     CDS             1540..>2176
                     /locus_tag="VNL92_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO93205.1"
                     /translation="MGKLDGKVAIVTGASRGIGKAIAELFAEEGGKVICSARTLREGE
                     HPLEGSLETTVNEIRSKGGEATPVPCDVSSEEDCERLVQQTKDIYGPCDVLVNNAALT
                     YFIPVVDFPTKRWKKSFEVNFHAPFILSKLVLPDMIARHSGAIVNISSGAAIGPGRGP
                     YDASAQMRGGTLYGAEKAALERFTQGLAEEVFAQGVSVTCVSPSQVVPTPGT"
BASE COUNT          400 a          748 c          698 g          330 t
ORIGIN      
        1 gttcttcgcc acgagcaggt ctcgcgccgc tgcggcatca cgctcctgga gcgggacggt
       61 gagctcatgg aggaactgat gcgcctcgtc gaagaatacg ttcctcgcgg cctccacgtc
      121 ttcacggccc gcgaggtccc gggcctcgca caacccccgc agggcgcggt cagcctcctc
      181 ttggccgatc gggctggcga ccgccgtgcc tccgctctcg gctgtcccgt cggagttgca
      241 ggctgcgagc accaatccgg ccagcagaac ggcaacgatt gcggagaggc gaaacggatg
      301 gcgacggaag agcatccgtc ccattaaggc acgcccagtc acgatcggac aagccgagcg
      361 acggagcggt cagcccacgg ccgcacggcc accatcggaa tccggcgacg gcgacgcctc
      421 gagcagccgc cgatagcaga catcgcggtg gcggccggtg aagcccatgc gatcgtacag
      481 ccggagcgcc ggcccattct cggcgtcggt gtagacgcac acatgccggc agccttgctc
      541 ggcaacatgc tgcacaccga gcgttatgag cgccgtcccg attccccggc ccgcccattc
      601 gggcgccacg cctatcacat agatctcggc cccgtccctc tccgcatcgc gccccacctt
      661 gagccagttg aaggcgatca tgcgcccttc cgcatccctc agaacgagga agtcgtccgg
      721 gtcgaaccac gcctccctga ggcgcgcctc gaggtcgaca tgcgtccagc gcccctgctc
      781 aggatggtgg gaaaaaacac gagcgttcag ggcaagccac tcccgatcgt cgatgccggg
      841 gcggaagcgg gccagacgca agcctgacgg caactgacac ggctcggcca cgtcccccgc
      901 gatatcgcgc cgcaggtgca acagcacacg ggccggccgg aacgcgtacg ccgcggcgag
      961 cgccctggcg ctcggagtgt ccatgtaggc ccaggcatcc atgtactgag cgccgtaccg
     1021 ttccgcgacg tcgaagaggt gacgcagcag aagattgccg acaccctgcc cgcgcgcctc
     1081 cgggtgcacc acgagctcgc aggccaaccg tgaaccgttc tcgtcggtga agaacacgga
     1141 ctgcgcatac cccatgagcc gctgctcctc ccaggcgagc accccgatcg acgtgtcgtc
     1201 gccgtgcgaa agccgcagga acttgtgctc gccgagagga ctatgaccgt cgtgctcggc
     1261 ggccgcctgc acgaggtcca ccacggcggg ctgaatctcc ttccccaact tcaggacgaa
     1321 ttcgatgtgg tgcatgcggc gaccgtacag ctcctcgact ttggggcgaa cctggagaga
     1381 cacatgagca ggagggctcc agctgcttcc attctcccac gagcgcgcca gcggcgggaa
     1441 ggcgaaggtg cccctcctca gccggcccgc gttgtgtcaa gatgggccac agtcacagga
     1501 agcgccgcgg ggcgcaagtc gagttcgtgg aggggagacg tgggcaagct cgatggcaag
     1561 gtagcaatcg taaccggcgc gagccgcggc attggcaaag ccatagccga actctttgcc
     1621 gaggaaggcg gcaaggttat ctgctcggcg cgaacgctgc gcgaggggga gcacccgctg
     1681 gaaggctcgc tcgagaccac tgtcaacgag atccgatcga agggcggaga ggccacgccg
     1741 gtcccctgcg acgtttcgag cgaggaggat tgcgagcgtc tcgtacagca gacgaaggac
     1801 atctacggcc cttgcgatgt actggtcaac aacgctgccc tcacctactt cattcccgtc
     1861 gtcgatttcc cgacgaagcg ctggaagaag tcgttcgagg tcaacttcca cgcgccgttc
     1921 atcctcagca agctcgtcct tccggacatg atcgcacggc actccggagc gatcgtcaac
     1981 atctcgtccg gtgccgcgat tggcccgggg cgtggcccct acgacgcatc cgcccagatg
     2041 cgaggcggca cgctgtacgg cgcggagaag gccgcgctgg agcgcttcac gcagggcctg
     2101 gcggaggaag tcttcgccca gggcgtctct gtgacttgcg tttcgccttc tcaggtcgtg
     2161 ccgacgccgg gcaccg
//
LOCUS       DATMBB010000019         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_554455, whole genome shotgun sequence.
ACCESSION   DATMBB010000019 DATMBB010000000
VERSION     DATMBB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554455"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VNL92_00405"
     CDS             <1..402
                     /locus_tag="VNL92_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HWO93206.1"
                     /translation="TGAHDDLRRATDLARQMVTRYGMSQTMGLATVEGPRMPLFLNTD
                     TSQRLECSEETSQMIDHEVRQLLEEARERVHRDLSATRATLEALAALLIEREVIDREA
                     LDGLMATGERTARAPATKEAPAAGEPRISAT"
     gene            420..680
                     /locus_tag="VNL92_00410"
                     /pseudo
     CDS             420..680
                     /locus_tag="VNL92_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
     gene            675..2975
                     /locus_tag="VNL92_00415"
     CDS             675..2975
                     /locus_tag="VNL92_00415"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWO93207.1"
                     /translation="MKRAGYRVGTARKRLSLRGLMCASSVKSIENALTATPGVLNARV
                     NVGTEEAEVEYLPAATDLARVKAAAGSAGYAVDETPPPVAEDPLAKEAAAREREYRTL
                     MRKWWFGAAVGVFTMIMSYPWLFPVLRDWFPRDSPQLWYIWAGMGVASLAVLLYSGNQ
                     FFIGAWQALKHRSANMHTLIALGTGVAWIYSTIALLFPQLFPGAEFTEVYYDVTVVVT
                     ALVVLGLAMELKAKGRTSDAIRKLIGLQAKTARVLRDGREMDIPVEEVLVEDVVVVRP
                     GEKIPVDGEITEGSSAIDESMVTGESLPVEKKVGDEVIGATMNKTGAFKFRATKVGKD
                     TALANIIRMVQDAQGSKVPVQRIVDIVSGYFTPAVTILAILGFMIWYTFGPPPALAYA
                     TIVAVTTLIIACPCALGMATPMSLTTGVGLGALNGILIRGGEALQTAQKLQTIILDKT
                     GTITHGKPALTDVVTTGAVREEELLRLAGGVEKSSEHPLAEAIVEGALARGLALSDPD
                     QFNAIPGHGIEARIENRSLLLGNVKLMRDRAIPLDGLEAQATRLADEGKTPMYVAIDG
                     RAAGIVAVADTIKPDSVEAIATLNRMGLETVMITGDNERTAKAIARQVGIERVLADVL
                     PQDKAFNVQRLQLEGKQVAMVGDGINDAPALAQADVGFAIGTGTDVAIAASDITLIKG
                     SLRGVVLAIQISRATMRNVYQNLFGAFIYNTLGLPIALGVLYPFLGILLSPLLAALAM
                     SFSSVTVIANANRLKLWKPRTAGALR"
     gene            2972..3349
                     /locus_tag="VNL92_00420"
     CDS             2972..3349
                     /locus_tag="VNL92_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWO93208.1"
                     /translation="MTPDQIVVNVIGLALIAFIVWFFWMKKAAGVRAAVTSAGYQEQM
                     VLVKGGYTPDVIVVERGRPVRLNFVRQESASCSEMVLLPAFNKSVNLPEGQTVPVEFM
                     PSEPGEYEFACQMGMFRGKVLVE"
BASE COUNT          605 a         1065 c         1130 g          567 t
ORIGIN      
        1 accggggccc acgacgacct ccgccgcgcc acggatctgg ctcgccagat ggtaacgcgc
       61 tacggcatga gccagacgat gggtctggcc acggtcgaag gcccacgcat gccgctgttc
      121 ctgaataccg acacgtctca aaggctggag tgcagcgaag aaaccagcca gatgatcgac
      181 cacgaagttc ggcagctgct cgaggaggcg cgcgaacgcg tgcatcggga cctcagcgcc
      241 acacgcgcca ccctcgaggc actggcggcc ctgctcatcg agcgcgaggt catcgaccgc
      301 gaagcgctcg atggactgat ggccaccggt gagaggaccg cgcgcgcgcc ggcgaccaag
      361 gaggcacccg cggccggaga gccgcggatc tccgcaacgt gagagacagg agcaacgcca
      421 tgacttcagc gacgacgatc agcaagccca cgcgagcgct ctccgcgacg accggcgcgc
      481 gcgacgatgt ggctggccct cgacgcattg agctcgacgt aggcggtctg aactgcggtg
      541 gatgcgcgct gatgctcgag ctcgccctcc gcggagtgcc gggcgtgcga tccgcgaccg
      601 tcaatctgac gagagctcgc gcgttcatcg actacgaccc gcagtggacg agcccggcgg
      661 cactggccga caccgtgaag cgcgctggct accgtgtcgg cacggccagg aagcgcttga
      721 gtcttcgcgg gttgatgtgc gcctcgtctg tgaagtcgat cgagaacgcc ctcacggcga
      781 cccccggtgt cctgaacgcg agggtgaacg tcgggacgga ggaggccgag gtcgagtacc
      841 tgccggccgc cacggatctc gcccgtgtca aggccgcggc cggttccgcc ggctatgcgg
      901 tcgacgagac accgcccccg gtggccgagg accctctcgc gaaggaagcc gccgcacgcg
      961 agcgcgagta ccgcacactc atgcggaaat ggtggttcgg cgcggcggtc ggggtcttca
     1021 cgatgatcat gagctatccg tggctcttcc cggtccttcg cgactggttc ccacgcgaca
     1081 gtccgcagct ctggtacatc tgggccggca tgggcgtcgc ctcgctggcc gtcctgctct
     1141 actcgggcaa tcagttcttc atcggcgcct ggcaggcgct gaagcaccgg tcggccaaca
     1201 tgcacacgct catcgcgctt gggacgggcg tggcgtggat ctattcaacg atcgcgctgc
     1261 tgttcccgca gctctttcct ggcgcggagt tcaccgaggt ctattacgac gtgaccgttg
     1321 tcgtaaccgc cctggtcgtg ctcgggctgg cgatggagct gaaggcgaag gggcgcacgt
     1381 cggacgccat ccgcaagctc atcggcctcc aggccaaaac ggctcgcgtc ctgcgtgacg
     1441 gccgtgagat ggacattccc gtcgaggaag tgctggttga ggacgtggtc gtcgttcggc
     1501 cgggcgagaa gatcccggtc gacggcgaga tcaccgaggg cagctcggcg atcgacgagt
     1561 cgatggtcac tggcgagtcg ctgccagtgg agaagaaggt cggcgacgaa gtgatcggcg
     1621 ccacgatgaa caagaccggc gcgttcaagt tccgggcgac caaggtcggc aaggacacgg
     1681 cgctcgccaa catcatccgc atggtccagg atgcgcaggg ctcgaaggta ccggtccagc
     1741 gcatcgtcga catcgtctct ggctacttca cgccggccgt gacgattctg gcgatcctgg
     1801 gcttcatgat ctggtacacg ttcgggccgc cgcccgcact cgcctacgcg acgatcgttg
     1861 ccgttacgac gctcatcatc gcctgcccgt gcgcgctggg gatggcgacg ccgatgtcgc
     1921 tcacgactgg cgtcggcctg ggcgcgctca acggcatcct gatccgcggc ggcgaggcgc
     1981 tgcagaccgc gcaaaagctg cagacgatca tcctggacaa gacgggcacg attacacacg
     2041 gcaagcccgc cttgaccgat gttgtgacga cgggcgccgt ccgcgaagaa gagctcctgc
     2101 ggctggcggg gggtgtggag aagtcttctg agcacccgct cgccgaggcg atcgtcgaag
     2161 gagccctggc ccgtggtctc gcgctgtccg atccggatca gttcaacgcg attccaggcc
     2221 atggcattga ggcgcggatc gagaaccgtt cgctattgct cggcaacgtg aaattgatgc
     2281 gcgaccgggc cattccgctt gacggtctgg aagcccaagc cacacggctg gcggacgagg
     2341 ggaagacgcc gatgtacgtc gctattgacg ggcgagccgc cggcatcgtt gccgtcgccg
     2401 acacgatcaa gcccgactct gtggaagcga ttgcgacgct caaccggatg ggtctcgaga
     2461 ccgtgatgat caccggcgac aacgagcgca cggccaaagc catcgcccgt caagtgggga
     2521 tcgaacgcgt actggctgac gtcctgccgc aagacaaggc cttcaacgtc cagcggctgc
     2581 agctcgaagg caagcaggtg gccatggtcg gggacgggat taacgatgcc ccggcgctgg
     2641 cccaggccga cgtgggattt gcgatcggca cgggaacgga cgtggcgatc gccgcctcgg
     2701 acatcaccct catcaagggc agcctgcgcg gcgtcgtcct ggcgatccag atcagccgcg
     2761 ccacgatgcg caacgtctac cagaacctgt ttggggcgtt catctacaac acgctgggtc
     2821 tgccgattgc ccttggcgtg ctgtatccct tcctgggaat cctcctgtcg ccattgttgg
     2881 cagcgctggc catgtccttc agctcggtga ccgtgatcgc caacgcgaat cgcttgaagt
     2941 tgtggaagcc tcgtactgca ggagcattgc gatgaccccc gatcagattg ttgtcaacgt
     3001 catcggcctg gccctgatcg ccttcatcgt gtggttcttc tggatgaaga aagccgccgg
     3061 tgtgcgggca gccgtcacga gcgctggcta tcaggagcag atggtgctgg tgaagggtgg
     3121 ctacacgccc gacgtgatcg tcgtcgaacg aggaaggccg gtccggctca acttcgtacg
     3181 tcaggagtcc gcgtcgtgca gcgagatggt gctactcccg gcgttcaaca agagcgtgaa
     3241 cctgccggag ggacagaccg tgcctgtcga gttcatgccc tcggagcctg gcgaatacga
     3301 attcgcctgc cagatgggca tgttccgggg gaaggtgctc gtcgaatagc ggtatctgga
     3361 atgactg
//
LOCUS       DATMBB010000020         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_554348, whole genome shotgun sequence.
ACCESSION   DATMBB010000020 DATMBB010000000
VERSION     DATMBB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554348"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..895)
                     /locus_tag="VNL92_00425"
     CDS             complement(44..895)
                     /locus_tag="VNL92_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO93209.1"
                     /translation="MMDGAESRVVVAARDYYNSDDADNFYFHVWGGEDIHIGLYESDE
                     EDIRRASRRTVERLAGRLADVGPRTSVLDLGAGYGGAARWLARRYRCHVTCLNLSEAQ
                     NRRNRAMNREAGLDDLIEVHAGNFERIPFEDESFGVVWSQDSFLHSGARETVLAAIDR
                     VLEPGGAVVFTDPMQSDDCPPGVLAPVLERIHLQDLGSFGFYRAVARELGWTEVGCED
                     LTRHLVRHYARVARELERQRARLEGVVSAAYIERMLRGLAAWVEAGERGYLAWGILHF
                     RKPPRLA"
     gene            complement(895..1713)
                     /locus_tag="VNL92_00430"
     CDS             complement(895..1713)
                     /locus_tag="VNL92_00430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93210.1"
                     /translation="MSIGTNIQGYGDDPLKVRATDQYIAEYVRSFVEKWDELIDWEAR
                     AQGEGGFFIEQLKAYGVKKVLDVATGTGFHSVQLLKAGFEVTSVDGSAVMLAKAFENA
                     RRHGFILRTIHADWRWLRRDVHDFHDAVICLGNSFTHLHNENDRRKALAEYYAALRHK
                     GILILDQRNYDALLDKQISPSHNYYYCGENVRAVPEYVDETLARFRYTFSDGSVYHLN
                     MFPLRKAYVQRLLREVGFQTVTTYGDFQETYRDREPDFFIHVAEKEYRNQGGEH"
     gene            1988..>2505
                     /locus_tag="VNL92_00435"
     CDS             1988..>2505
                     /locus_tag="VNL92_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWO93211.1"
                     /translation="MCLIGRNGAGKSTTLKLVTGELEPDEGTVERPSSLRFSVLDQKL
                     ADGSDVTVREFVARGMEAHLARIAAYQRLAAERQPDAATLREMESLEREILAGGGWSV
                     DAQIDAAVSDLHLQPEQRLSELSGGWRRRVALAQALVSKPELLILDEPTNHLDITTIE
                     WLERVVTRYEGAV"
BASE COUNT          417 a          854 c          796 g          438 t
ORIGIN      
        1 gcggccggcg cgcgattggc accggccacc gccgttcagc tgatcaggcg aggcgcggcg
       61 gcttgcggaa gtgcaggatg ccccaggcga ggtagccgcg ctcgcccgcc tccacccagg
      121 cggccagccc acgcagcatg cgctcgatgt acgccgccga caccacgcct tcgagccgcg
      181 cgcgctgccg ctcgagctcg cgcgcgaccc gcgcatagtg ccggacgagg tgccgcgtga
      241 gatcctcgca cccgacttcc gtccagccga gctcgcgggc gacggcgcgg tagaagccga
      301 acgacccgag atcctgcaga tggatgcgct cgagcaccgg cgcgagcacg cccggagggc
      361 agtcgtcgga ctgcatcggg tcggtgaaca cgaccgcgcc gccaggctcg agcacacggt
      421 cgatcgcggc cagcaccgtt tcgcgggccc cggaatgcag gaacgagtcc tgcgaccaga
      481 cgacgccgaa gctttcgtcc tcgaacggta tccgctcgaa attgccggca tgcacctcga
      541 tgagatcgtc gaggccggcc tcgcgattca tggcgcgatt gcggcggttc tgcgcctcgc
      601 ttagattgag gcaggtcaca tgacagcgat agcggcgcgc gagccacctc gcagcgccgc
      661 cgtagcccgc gccgaggtcc agcacgctgg tccgcggtcc cacgtctgcc agccgccccg
      721 caagccgctc cacggtgcgc cgggacgccc gcctgatgtc ctcctcgtcg gactcgtaga
      781 ggccgatgtg gatgtcctcg ccgccccaca cgtggaagta gaagttgtcc gcatcgtcgc
      841 tgttgtagta gtcccgcgcg gcgacgacga cgcggctctc ggcgccgtcc atcatcaatg
      901 ctcccctcct tggttcctgt actccttctc cgccacgtgg atgaagaagt ccggctcacg
      961 gtctcggtac gtctcctgga agtcgccgta cgtcgtcacg gtctgaaacc ccacctcgcg
     1021 aagcaatcgc tgcacgtaag ccttgcgcag cggaaacatg ttgaggtggt acaccgagcc
     1081 gtcgctgaag gtgtagcgga agcgcgcgag cgtctcgtcc acgtactcgg gcacggcgcg
     1141 cacgttctcg ccgcagtagt agtagttgtg cgacggagat atttgcttgt cgagcaacgc
     1201 gtcgtagttg cgttgatcga ggatcaggat tcccttgtgt cgcagagcgg cgtagtactc
     1261 ggcaagcgcc ttgcggcgat cgttctcgtt gtgcaggtgg gtaaacgagt tgccgaggca
     1321 gatcaccgcg tcgtggaagt cgtgcacgtc gcgcctgagc cagcgccaat ccgcgtggat
     1381 ggtgcgcagg atgaacccgt gtcgccgggc attctcgaac gccttcgcca gcatcaccgc
     1441 gctgccatcc acgctcgtga cctcgaagcc ggccttcagc agctgcacgg agtgaaagcc
     1501 cgtgcccgtg gcgacgtcca agactttctt caccccgtag gccttgagct gctcgatgaa
     1561 gaacccgccc tcaccttgcg cgcgggcctc ccagtcgatc agctcgtccc acttctcgac
     1621 gaacgatcgg acgtattccg ctatatactg gtccgtcgcg cgtaccttca gaggatcgtc
     1681 gccgtagccc tgaatgttag tgccaatgct catgtgtcta tgctctctcg ttttgttgtc
     1741 ggtgagcacg gaaactgata gttccgcaca tggaaacgac cgaaggaatc ggccgcggcg
     1801 aggttcccat ggtaacgcgt gcacgctcga tgatctaccc tgtcgtgcat ttgctcttgc
     1861 catcacgact attctgctcc gcgaatagcg catgacccca ctcgttcgtt tcgacgccgt
     1921 caccttctct tttggggatc aacgcatcct cacggaggcc gagctcacca tcgagcccgg
     1981 tgagcgagtg tgcctgatcg gccgcaacgg cgccggcaaa tccaccacgc tgaagctcgt
     2041 cacgggcgag ctcgagcccg acgaaggcac cgtggagagg ccttcgtcgc tacgcttcag
     2101 cgtgctcgat cagaagctcg cggacggctc cgatgtcacg gtgcgggagt tcgtggcccg
     2161 aggcatggag gcgcacctcg cgcggatcgc cgcttaccag cgcctcgccg ccgagcggca
     2221 gccggacgcg gccacgctgc gcgagatgga gtcgctcgag cgcgagatcc tcgcgggcgg
     2281 cggctggtcc gtcgacgcgc agatcgacgc ggcggtgagc gatctgcatc tgcagcccga
     2341 gcagcgcctg agcgagctgt cgggcggctg gcgtcgccgc gtggcgctcg cgcaggcgct
     2401 cgtctcgaag cccgagctgc tgatcctaga cgagcccacc aatcacctcg acatcaccac
     2461 catcgagtgg ctcgagcgcg tcgtcacgcg ctacgaaggc gccgt
//
LOCUS       DATMBB010000021         4707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_276174, whole genome shotgun sequence.
ACCESSION   DATMBB010000021 DATMBB010000000
VERSION     DATMBB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276174"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VNL92_00440"
     CDS             complement(<1..495)
                     /locus_tag="VNL92_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWO93212.1"
                     /translation="MFLDAIKFEHTVFALPFAYIGMVLADDTPAWTDVVWITVAMFAA
                     RTVAMGTNRIVDRHQDAANPRTRTRALPAGLLSMREMTVFTIAAAVLFFVAAGALNRT
                     ALALAPVALILLVTYPYAKRYTWLCHWYLGIADGIAPAAGWIAVTGSLELTPVLLFVA
                     VTVWV"
     gene            complement(552..2366)
                     /locus_tag="VNL92_00445"
     CDS             complement(552..2366)
                     /locus_tag="VNL92_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HWO93213.1"
                     /translation="MALHSFRDYLQLLEQQGELRRVRVEVDPVLEVSEIAQRVVREAG
                     PALLFENVRGSRHPLAINFFGSERRIELALGRHPGEFGSGLIELLERLNPPTPRALWS
                     AKGELPRLLAARVRTRATGPCREIEERPSLHSLPVIQCWPKDAGRFITYPLVLTHHPQ
                     TRRRNLGLYRMQLFDECSTGMHWQIQKGGGFHYAQAELLGRSLPVAVALGADPATMIA
                     AVVPLPEDIDELAFAGFLRGSPVTLARGRSIPVAAPAEAEFVLEGVVPAGERREEGPF
                     GDHFGHYSAAAPFPVFHVRGLTRRRNAVFPAAVVGKPPQEDTYLGNATQELTGPLIRL
                     LMPEVTGVWAYYEAGFHNLLAAAVHTRYPKEAMKSAMGLLGQGQLSLTKCLILVDAGV
                     DVRDFRAVLRQIALHVDPRDDFVLIPNAPLDTLDFTSFKMHLGSKMIIDATSPRYRSA
                     PVDDEGGSVEPGPSSEAMAAAQIDRLPADLRSQVDGWALHEDTLLAVRTCGDHGRDLL
                     RALLAVSDSLSPKLLAVVSGDVDVTDRVQLIWGIFTRFDPGRDVLFPSSRFVGSWPIH
                     EGQLGIDATWKPGYPEPLEMSQEIRDLVDRRWQEYWRT"
     gene            complement(2377..>4707)
                     /gene="uvrA"
                     /locus_tag="VNL92_00450"
     CDS             complement(2377..>4707)
                     /gene="uvrA"
                     /locus_tag="VNL92_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWO93214.1"
                     /translation="IVLEKNRRHDIDIVVDRLVTPSPDEAEDAEAGSQTSSMRITDSV
                     ETALKLGAGVVKVAVEGGEERLFSEHFACVECGISLGEIAPRTFSFNSPHGACPECTG
                     LGVKLEIDPELVVPNRDLSLAQGAIEPWARSGTTSMWYMRMLEAAAEAFGFSVDTPVR
                     DLPADALRTVLYGSEQRVRLRFSHHGDGHGYWSNFEGVIPNLERRHRETESDYVRQEI
                     ERYMAANPCPACHGQRLKPEALAVLIGERNIVGVTSMTTLEAREWIEGLAAPESPLGP
                     RERAIAGQIIKEIRERLTFLVDVGLEYLSLDRASATLSGGEAQRIRLATQIGSSLMGV
                     LYICDEPSIGLHPIDDDRLIRTLKRLRDLGNTVIVVEHDEAMMRAADHIVDMGPAAGE
                     HGGRVVAEGPIEAIMASPTSLTGQYLRGARRIPMPEGRRPGTGEVLVVRGARENNLKN
                     IDVSIPLGKFVCVTGVSGSGKSTLISDILARKAAQVLYGARERPGECDRIDGLELIDK
                     VVNIDQSPIGRTPRSNPATYTGTFTPIRELFATVPEARVRGYGPGRFSFNVKGGRCEA
                     CRGDGYIEIEMNFLPDVTVPCEVCKGQRYSREALEITFRGKNIAEVLDMTVDEAVEFF
                     GAFPKVRNKLATLQGVGLGYIRLGQPATTLSGGEAQRVKLSTELSRRSTGRTLYILDE
                     PTTGLSFEDTAALLRVLQAFVDGGNTVVVIEHHLDVIKNADHIIDLGPGGGDKGGYVI
                     AEGPPEDIARVEGSATGMYLGRVLESGTAVPAAGVA"
BASE COUNT          795 a         1529 c         1609 g          774 t
ORIGIN      
        1 cacccagacc gtgacggcga cgaacaacag cacgggcgtc agctcgagcg agccggtcac
       61 ggcgatccag ccggctgccg gagcgatacc gtcggcgatg ccgaggtacc agtgacagag
      121 ccaggtgtaa cgcttcgcat aggggtacgt cacgagcaga atgagcgcga caggcgccag
      181 cgccagcgct gtccggttca gcgcccctgc cgcgacaaag aagaggactg cggcggcgat
      241 ggtgaacacg gtcatctccc gcatggagag gagaccggcc ggcagcgcgc gggtccgggt
      301 gcggggattc gcggcatcct ggtgcctgtc gacgatgcga ttcgttccca tcgccacggt
      361 gcgggccgcg aacatggcta cggtgatcca gacgacgtcg gtccacgcgg gagtgtcgtc
      421 ggcgagcacc atgccgatgt aggcgaaggg cagggcgaac accgtgtgct cgaacttgat
      481 cgcgtcgaga aacacgcgta ggcggtgagc cgctcgcgga aatcctgatc cgccagtgtg
      541 ctggttggat gttacgttcg ccaatactcc tgccacctgc gatcgacgag atcgcgaatc
      601 tcctgggaca tctcgagagg ctccgggtac ccgggcttcc acgtcgcatc gatgccgagc
      661 tggccctcgt ggatcggcca ggacccgacg aatcgactcg aggggaagag cacgtcgcgg
      721 cccgggtcga atcgcgtgaa gatgccccag atgagctgca ctcgatccgt cacgtcgacg
      781 tcgccgctga cgacagcgag aagcttgggc gaaaggctat ccgaaacggc gagcagggcg
      841 cgcaacaggt cacggccgtg gtcgccacac gttcggaccg ccagcagggt gtcctcgtgg
      901 agggcccacc cgtcgacctg cgagcgcagg tccgcgggga ggcggtcgat ctgcgctgcc
      961 gccatcgcct ccgaggaggg cccgggctcg accgagcctc cctcgtcgtc cacaggggcg
     1021 gaccggtagc gaggacttgt cgcatcgatg atcatcttgg agccaaggtg catcttgaag
     1081 ctcgtaaagt cgagcgtatc gagcggcgcg tttggaatga ggacgaagtc gtcccgcggg
     1141 tcgacgtgca gggcaatctg ccgcaggacg gcgcggaagt cacgcacgtc cacgccagca
     1201 tcgacgagaa tgaggcactt cgtgagcgag agctggcctt ggccgaggag tcccatcgcc
     1261 gacttcattg cctccttggg atagcgcgtg tggacggcgg cggccagaag gttgtggaat
     1321 cccgcttcgt agtaggccca cacgccggtg acctccggca tgaggaggcg gatgagcgga
     1381 ccggtgagct cctgtgtagc gttgcccaga taggtgtctt cttgcggcgg cttcccgacg
     1441 acggcggcag ggaagactgc gttgcgcctc cgcgttaggc cacgaacgtg gaacactggg
     1501 aacggcgccg cggccgagta gtgaccgaag tgatcgccaa aaggcccctc ctcgcgtcgc
     1561 tcgcccgcgg ggacaacgcc ctcgaggacg aactcggcct ccgcaggcgc cgcgacgggg
     1621 atacttcgac ctcgcgccaa ggttaccggc gacccgcgaa ggaaacccgc aaaggcgagt
     1681 tcgtcgatgt cctccggtaa cggcacgacg gcggcgatca tcgtcgcagg gtcggcgcca
     1741 agggccaccg cgacaggcag cgatcgccca agcagctcgg cctgcgcgta atggaagccg
     1801 ccgcccttct ggatctgcca gtgcatgccg gtggagcatt cgtcgaagag ctgcatccgg
     1861 tacaggccca ggttgcgacg tcgcgtctgg gggtgatggg tgaggacgag cgggtaggtg
     1921 atgaaccggc ccgcatcctt cggccagcac tgaatcacgg gtagcgagtg gaggctgggc
     1981 cgttcctcga tctcgcggca cggacctgtg gcgcgagtgc ggacgcgcgc tgctaggagt
     2041 cgagggagct cgcccttggc cgaccagagc gcgcgaggcg tcggcggatt gagtcgctcc
     2101 aggagctcga tcagcccgct cccgaactcg ccgggatgcc ggccgagcgc gagttcgatg
     2161 cggcgttcgg agccgaagaa gttgatggcc agcgggtgcc tcgagccgcg cacgttctcg
     2221 aacaggagcg ccggacctgc ctcgcgcacc acccgctgcg cgatttcgga gacctcgagg
     2281 acggggtcaa cttccacgcg gacgcgccgc aattcgcctt gctgctcgag cagctgcagg
     2341 tagtcgcgga agctgtgtag agccatgctc tgcggctcag gccacgccgg cagctggcac
     2401 ggcagtcccg ctttcgagga cgcggcccag gtacatgccg gtcgcgctgc cctcaacacg
     2461 ggcgatgtcc tccggcggcc cctcggcgat gacatatccg cccttgtcgc cgccgcccgg
     2521 ccctaggtcg atgatgtggt ccgcgttctt gatgacgtcg aggtggtgtt cgatgacgac
     2581 aaccgtgttc ccgccatcga cgaaggcctg caggacccgg agcaacgcgg ccgtgtcctc
     2641 gaacgagagg cctgtcgtcg gttcgtccag gatgtacagc gtccgcccgg tggagcgccg
     2701 cgacagctcg gtcgacagct tgacccgctg cgcctcgccg ccggagagcg tcgtggccgg
     2761 ctggcccagg cgaatatagc cgaggccgac gccctgtagc gtggcaagct tgttccgtac
     2821 cttgggaaag gccccgaaga actcgacggc ctcgtcgacg gtcatgtcga gcacttcggc
     2881 gatgttcttg ccccggaacg tgatctcgag cgcctcgcgg ctatagcgct gccctttgca
     2941 tacttcgcac gggaccgtta cgtccggcag gaagttcatc tcgatctcga tgtagccatc
     3001 gccgcgacac gcttcgcagc gaccgccctt cacattgaag gagaagcgtc ccgggccgta
     3061 gccccgcact cgtgcttcgg ggactgtcgc gaacagctct cggatcggcg tgaaggttcc
     3121 ggtgtaggtg gcggggttcg agcgaggagt gcggccaatc ggcgattggt cgatgttgac
     3181 caccttgtcg ataagctcga gtccgtcgat gcggtcgcac tcacccggtc gctctcgtgc
     3241 cccgtacagt acttgggctg ccttgcgcgc caggatatcg gagatgagcg tgctcttgcc
     3301 cgagccggat acaccggtga cgcagacgaa cttcccgagg gggatgctga cgtcgatgtt
     3361 cttcaggttg ttctcgcgcg cgccacggac gacgagcact tcgccggtgc ccggccgccg
     3421 accctcgggc atcgggatgc gccgcgctcc gcgcaggtac tgcccggtga gggaggtggg
     3481 gctcgccatg atagcctcga tcggcccctc ggcgacgaca cggcccccgt gctctcccgc
     3541 cgcaggaccc atgtcgacga tgtgatccgc tgcccgcatc atcgcctcgt cgtgttcgac
     3601 gacgatgacc gtgttcccga ggtcgcgcag acgcttgagc gtgcgaatga gccggtcatc
     3661 gtcgatcgga tgcagcccga tcgacggttc gtcacaaatg tagagcacgc ccatgaggct
     3721 ggagccgatc tgcgtggcga gacgaatgcg ctgcgcctcg ccgccggata gcgtcgcgga
     3781 ggcgcggtcg agcgagaggt actcgagccc gacatcgacg aggaacgtga gccgctcgcg
     3841 gatctccttg atgatctggc ccgcgatggc tcgctcgcgg ggcccaagcg gcgactcggg
     3901 cgccgcgaga ccctcgatcc actcgcgcgc ctcgagcgtc gtcatcgacg tgacccccac
     3961 gatgttgcgc tcgccgatca ggacagcgag cgcctccggc ttcagacgct gaccgtggca
     4021 tgccggacag gggttcgccg ccatgtaccg ctcgatctcc tggcgaacgt agtccgactc
     4081 cgtctcacga tggcgccgct ccaggttggg gatgacgccc tcgaagttcg accagtatcc
     4141 gtgcccgtcc ccgtgatggc tgaagcgaag gcgcacgcgc tgctcggagc cgtacagcac
     4201 ggtacgtagc gcatcggcgg gcaggtcgcg aacgggcgtg tcgacggaga agccgaacgc
     4261 ctcggcggcc gcctcgagca tgcgcatgta ccacatgctg gtcgtcccgc tgcgagccca
     4321 cggctcgatg gccccctgtg cgagcgagag gtccctattc ggaacgacga gctcggggtc
     4381 gatctccagc ttcacgccca acccggtgca ctcggggcag gcgccgtggg ggctgttgaa
     4441 gctgaacgtg cgtggcgcga tttcgccaag gctgattccg cactccacgc aggcgaagtg
     4501 ctcgctgaac agacgctcct cgccaccctc gacggcaacc tttacgacac ccgcgccgag
     4561 cttgagcgcc gtctcgacag agtcggtgat acgcatgctg cttgtctggg agccggcctc
     4621 tgcgtcctcg gcctcatccg ggctcggcgt cacgagccgg tcgacgacaa tgtcgatgtc
     4681 gtgtcgcctg ttcttctcga gcacgat
//
LOCUS       DATMBB010000022         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_194107, whole genome shotgun sequence.
ACCESSION   DATMBB010000022 DATMBB010000000
VERSION     DATMBB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194107"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1667
                     /gene="purH"
                     /locus_tag="VNL92_00455"
     CDS             147..1667
                     /gene="purH"
                     /locus_tag="VNL92_00455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWO93215.1"
                     /translation="MRALLSVYDKSELVPFARALAQLGWELISTGGTKKALDEAGIPT
                     LSVADVSGSPEILDGRVKTLHPKIHGGILARRDLPEHLEQLRRHDIPPIDLVVANLYP
                     FVETVRRPGVSLREALENIDIGGPAMIRAAAKNFPHVLVVVDPRDYPPLLAMLRKGAV
                     SQDERRRLARKAFAHVAMYDSVVSEFLAGESDPFAEQLTLPLRKIEDLRYGENPHQRA
                     AFYAIESSEPEQRGVPAAEQLHGKQLSYNNILDADAAWNAALDFAEQTVVIVKHTNPC
                     GVASDEDQAQAYREALRGDPISAYGGIVAINRKLGSAAAKELAKTFYEIVIAPDYEPE
                     ALDLLRKKRDLRILRIPSARPGRGPSYRYIEGGALVQTPDIEDAVDLRIVTERAPTDE
                     ERAALLFAWRCVKHVKSNAIVISGERALYGMGAGQPNRVTSVELALARAGERAKGAVL
                     ASDAFFPFADGVEAAGRGGIRAIIQPGGSIRDEEAIQAANSAGMAMVFTGLRHFRH"
     gene            1736..>2785
                     /locus_tag="VNL92_00460"
     CDS             1736..>2785
                     /locus_tag="VNL92_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93216.1"
                     /translation="MTDRPSIADIDRLLETLPPESRTLFDRLFLVDVAYGETDPPAEM
                     HQWVREQFGGVEDVRRQKVVKITNRATLQGAVFNDLRSRRPQHLEDPPSIDDELAATD
                     DPLARPEQMTSADTFGRVRGRYCITSSNVAKFDAMHGLVIFDEPHPLRFTREQVCDYF
                     ETARRWLMRAHEARPAATYPLVVWNCLWRAGASLIHGHLQVLLGESRHYPVVERLRRD
                     ARMYAEDSGRSYFEDLFSAHAALGAGFELGGVRIMANVAPVKEREVILLSDEWDDALE
                     QAIADVLAYLRDREGTASFNLALAAPPMGEPEAAWRDFPIVVHILDRGSLRTRTADVA
                     GMELYAASVVSSDPLQ"
BASE COUNT          462 a          931 c          938 g          454 t
ORIGIN      
        1 gtcttcaacc tcggtctcgg cctcgtggtc gcgttcaccc ccgccgatgc cgcagcgttc
       61 gtaggcgctg tgcccgaggc gatccccgtc ggcgaagtgc aagaacgcgc acccggcctg
      121 cccgcgttct cgctcgaagg cctgccatga gagcgctcct cagcgtctac gacaaatcgg
      181 agcttgttcc attcgcccgt gcactcgcac aactgggctg ggagctcatc agcaccggcg
      241 gaaccaaaaa ggccctcgac gaggcgggca ttccgacgct ctcggtcgca gacgttagcg
      301 gctcgccgga gattctggat ggccgcgtga agaccctgca cccgaagatc cacggcggca
      361 ttctggccag gcgcgacctc ccggagcacc tggagcagct gcggcgccac gacattccgc
      421 ccatcgacct ggtggtggcg aatctctatc cgttcgtcga gacggtgcgg cggccgggcg
      481 tgtcccttcg ggaggctctc gagaacatcg atatcggcgg cccggcaatg attcgggcgg
      541 cggcgaagaa cttcccccac gtgctcgttg tcgtcgatcc gcgggactat cctccgctgc
      601 tggcgatgtt gcgcaagggg gccgtgtcgc aggacgagcg acgacgcctg gcgcgcaagg
      661 ccttcgccca cgtggccatg tacgactccg ttgtgagcga gttcctcgcc ggcgagtccg
      721 atccgttcgc ggagcagctc acgctccccc tccgcaagat cgaggacctg cgctacggag
      781 agaacccgca ccagcgggcc gccttctacg cgatcgagtc atcggagccc gagcagcgcg
      841 gagtgccggc ggccgagcaa ctgcacggca agcagctttc gtacaacaac atcctcgacg
      901 cggacgcggc atggaatgcc gcgctcgatt tcgccgagca gaccgtcgtc atcgtgaagc
      961 atacgaatcc gtgcggcgtt gccagcgatg aggaccaagc ccaagcgtac cgcgaggcgc
     1021 tccgaggcga tcccatctcg gcctacggtg gcatcgtcgc gatcaatcgg aagctgggaa
     1081 gtgcggcggc gaaggagctg gcaaagactt tctacgagat cgtcatcgcg cccgactatg
     1141 agcccgaagc gctcgacctg ctgcgcaaga agcgcgattt gcgcatccta cgcataccgt
     1201 cggcgcgtcc ggggcggggg ccgagctacc gctacatcga gggcggcgcg ctcgtgcaga
     1261 cgccggacat cgaggacgct gtagacctgc gcatcgtcac ggagcgggcc cccacggatg
     1321 aagagcgcgc agccctcctg ttcgcgtggc gctgcgtgaa gcatgtcaag tcgaacgcca
     1381 tcgtcatcag cggcgagcgt gcgctctacg ggatgggcgc gggtcagccg aaccgcgtga
     1441 ccagcgtgga gctcgccctc gcgcgggccg gcgagcgtgc caagggcgcc gttctcgcct
     1501 ccgatgcgtt ctttcccttc gcggatgggg tcgaagcggc gggccgcggg ggcattcgcg
     1561 ccatcatcca gcccggcggg tcgattcgcg acgaagaggc gatccaggcc gcgaattcgg
     1621 cggggatggc gatggtcttc accgggctcc ggcacttccg ccattaggcc aacgccgacg
     1681 ccaagggctt aaagaaggct ggccgctggc gccgacttcg gccagaatgg acgctgtgac
     1741 cgatcgcccg agcatcgctg acatcgaccg tctgctggag acgctgccac cggagtcgcg
     1801 caccctcttc gaccggctgt tcctcgtcga tgtggcctac ggcgagaccg atccgccggc
     1861 ggagatgcac cagtgggtcc gcgagcagtt cggtggcgtg gaggacgtgc gtcggcagaa
     1921 ggtcgtcaaa atcacgaatc gagcgacgct ccagggcgcg gtcttcaacg acctgcggtc
     1981 gcggcggcct caacatctcg aggacccgcc ctccatcgat gacgaactgg cggccacgga
     2041 cgacccgctc gcgcggccgg agcaaatgac atcggctgac acgttcggtc gggtgcgtgg
     2101 gcggtactgc atcacgtcct cgaatgtggc gaagttcgat gcgatgcacg gcctcgtcat
     2161 tttcgacgaa ccgcacccgc ttcggttcac gcgagagcag gtgtgcgact acttcgagac
     2221 cgcgcgccgt tggctgatgc gagcccacga agcgaggcca gcagcgacct accccctcgt
     2281 cgtgtggaac tgcctctggc gggccggggc ttcgctcatc cacggacacc tgcaggtcct
     2341 gctaggggag agccggcact atccggtggt ggagcggctg cgccgtgacg cgcggatgta
     2401 cgccgaggat tcgggccggt cgtacttcga ggacctgttc tcggcgcatg cggcgctggg
     2461 cgcgggcttt gagcttggcg gtgtccgcat catggccaac gtcgcgcccg tcaaggagcg
     2521 ggaggtcatc cttctatcgg acgagtggga tgacgccctc gagcaggcca tcgccgacgt
     2581 gctcgcctac cttcgcgatc gcgagggaac ggcgtccttc aatctcgccc tggccgcccc
     2641 cccgatgggc gagcccgagg ccgcatggcg cgactttccg atcgtcgtgc acattctcga
     2701 tcgcggcagc ctgcgcaccc gcacggcgga cgttgccgga atggagctct atgcggcgag
     2761 cgtcgtgagc agcgatccgc tgcag
//
LOCUS       DATMBB010000023         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_193751, whole genome shotgun sequence.
ACCESSION   DATMBB010000023 DATMBB010000000
VERSION     DATMBB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193751"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VNL92_00465"
     CDS             complement(<1..580)
                     /locus_tag="VNL92_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93217.1"
                     /translation="MAEEADQVPADLLEKALSKGWSEELVRASLERGFLPEQLHAALD
                     SGMTAEDAQARISGGGFKLSMDWIQGTSEWGIRAKPTKKGLTLSAINVGTYGEIPDVW
                     ENQTEMPRGASPISGVQSMGYSLYQKADLWADSCPDLYEEAIQRRWRPAVDIPWDTMQ
                     PLPDDIERAMCQICTEVSEYALVTMDVLGKWLK"
     gene            complement(739..1806)
                     /locus_tag="VNL92_00470"
     CDS             complement(739..1806)
                     /locus_tag="VNL92_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWO93218.1"
                     /translation="MAVDVDTLQMLRTEPEVPHADLSWLNLDTAWGMTAKPGKRGLTL
                     DEINVGAYGDVPDQTTNYSFRPRGAAARREAPRSGMPYVDRADVWSQNAALLYEEAVA
                     RQWSSATDIPWEQLEPLPDDLEQAFCQFCTFLTEVEFIAGDTPGQWLPFISNDYYESK
                     LFLASQIVDEARHLDVFRKRALANGGGLLQQGFGGGLRNIIDAKDFTEMSALMHIVGE
                     GFVQSIFRMGEYIAQNEVEKRIFRLCGQDESRHLAFGVLHLKYVLETQPWRREEIHSY
                     LDKAEGGQLDEGIATSRATSECLAILMGGGKDNIDEGIKKLMVARKRQINEYMHRLEV
                     AGLADRKDRMNPAFGAFLDVN"
BASE COUNT          353 a          660 c          631 g          436 t
ORIGIN      
        1 ccttgagcca tttgcccaac acgtccatcg tgacgagcgc atactcgctt acttccgtgc
       61 atatttggca catcgctcgt tcgatgtcat cgggaagggg ctgcatcgta tcccagggga
      121 tatcgaccgc cggtcgccag cgccgctgga tggcttcctc gtagagatcg gggcagctat
      181 cggcccagag gtccgccttc tggtacagcg agtatcccat gctctgcacg ccggaaatcg
      241 gcgaggcgcc ccggggcatc tccgtttggt tttcccaaac atccgggatc tcgccgtagg
      301 tgccgacgtt gatggcggag agcgtgagac ccttcttcgt tggtttggcc cggatccccc
      361 actcgctcgt tccctgaatc caatccatgg agagcttgaa tccgccccca gagattcgcg
      421 cctgagcgtc ctcggccgtc attcccgaat cgagcgcggc gtgaagttgt tcggggagga
      481 accctcgctc gaggctggcg cgaacaagct cctcgctcca gcccttcgag agcgcctttt
      541 ccaataggtc ggctgggacc tggtccgctt cttctgccat caagtgctcc ttcgccgtca
      601 ggcgcggccg cccgtctccg acggtccggc ctcacgcagg atgatcttgc cgatgcacgg
      661 ctgattcgtc aacctggttg caacgcaagc cctccgccga agacttcgac ggagggccag
      721 ggcgatgaac tccgatcgct agttcacgtc gaggaaggcg ccgaaggctg ggttcatgcg
      781 atccttccga tctgccagcc cagccacctc gaggcggtgc atgtactcat tgatctgacg
      841 cttgcgcgcg accatgagct tcttgatgcc ctcgtcgatg ttgtccttgc cgccgcccat
      901 caagatggcg aggcattccg atgtggcccg gctcgtggcg atgccctcgt cgagctgacc
      961 gccctccgcc ttatcgagat aggagtggat ctcctcgcga cgccacggct gcgtttcgag
     1021 aacgtacttc aggtgcagca caccgaaggc taggtgacgg gattcgtcct ggccgcacag
     1081 acggaagatg cgcttctcca cctcgttctg ggcgatgtac tcgcccatgc ggaagatgct
     1141 ctggacgaag ccctcgccga cgatgtgcat gagagccgac atctcggtga agtccttcgc
     1201 gtcgatgatg ttacgcaggc cgccgccaaa gccctgctgg agcagcccac cgccgttggc
     1261 cagggctcgc ttccggaata cgtcgaggtg acgcgcctcg tcgacgatct ggctggcgag
     1321 gaagagcttc gattcgtagt agtcgttgct aatgaagggg agccactggc cgggcgtgtc
     1381 tccggcgatg aactcgacct cggtgaggaa cgtgcagaac tgacagaagg cttgctcgag
     1441 atcatcggga agcggctcaa gttgctccca cggaatgtcg gttgccgagc tccactggcg
     1501 agccaccgcc tcctcgtaga gcagcgcggc attctgcgac cagacgtccg cccgatcgac
     1561 atagggcatg ccggagcgtg gtgcttcacg ccgggccgcc gcaccccggg gccggaagct
     1621 gtagttcgtc gtttggtcgg gcacatcgcc gtacgcgccg acgttaatct cgtcgagcgt
     1681 gagcccgcgc ttgcccggct ttgcggtcat gccccaggca gtatcgaggt tcagccagct
     1741 gaggtcggcg tgtggaacct ccggctccgt tcgaagcatc tgcagcgtat cgacatctac
     1801 agccatgttc gttcccctct tctggtatag ggtttcctga tcttcccaac acctgtttgg
     1861 cgacgatgag catataagac ctgatatagc ctgtcaagcc gtactgtgtc ggtgtcgtgg
     1921 cctactcgtt ccagcagttc ttgaccgcct ttcgtcagct gcggtagcct tctcgcgccg
     1981 gggcgcgtca gcctcagcgc ggcgcaagaa ggagggaaac gggggctctc tatgtccatc
     2041 gagcgcgagg ttcaatccac attcgagcgt tacgctgcag
//
LOCUS       DATMBB010000024         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_193666, whole genome shotgun sequence.
ACCESSION   DATMBB010000024 DATMBB010000000
VERSION     DATMBB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193666"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VNL92_00475"
     CDS             complement(<1..412)
                     /locus_tag="VNL92_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWO93219.1"
                     /translation="MTSAPWRERKPDWIKVRFPAGENFHRVKALVRKHNLHTVCEEAH
                     CPNIGECFGRGTATFMILGETCTRACGFCAVTSGRPSGLDEAEPERLAASVRILGLRY
                     VVITSVNRDDLPDGGAHIFARCLERIREDAPDVAV"
     gene            complement(409..1080)
                     /gene="lipB"
                     /locus_tag="VNL92_00480"
     CDS             complement(409..1080)
                     /gene="lipB"
                     /locus_tag="VNL92_00480"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWO93220.1"
                     /translation="MRQRCLIHRAGLVDYEAAWRQQHELLAAVQAGGQHQLLLLQHPP
                     TFTLGRNGHDAHVLASEEMLLARGARLFRVDRGGDVTFHGPGQIVGYPILDLRRLWGT
                     ADIPQYVRLLERVISGTLEEYGIAAGCVPGRAGVWVGDEKIAAIGVRVSRGVTMHGFA
                     LNVDPDLSFFELIVPCGIADAGVTSMARLLGAPPPLDEVADRLARRFSDVFEVDLLAP
                     AEAVL"
     gene            complement(1195..>2290)
                     /locus_tag="VNL92_00485"
     CDS             complement(1195..>2290)
                     /locus_tag="VNL92_00485"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWO93221.1"
                     /translation="VIISAGFREAGEAGVEREQAVLDIARRYGMRLVGPNSLGVIDAL
                     HGLNVSFSDGLPPPWEVGVFSHSGAVASAILDWSVQTRNGFSKFISLGNMADVTEVDV
                     LRAWTADPEVRCAVAYLEGIRDGRAFFNAARDFTARKPLIAMKVGTTQGGRAAAFSHT
                     GALAGSERVVDAALRQSGIVRALTMEELFDFTRCFAFAPLPAGPNVAIVTNAGGPGVM
                     AADAVERLGLRLAGLSGETIERMRQGLPAAASLRNPVDLIGDADADRYRLALEAIAED
                     EAVHGIIALLTPQAVTEPEATARIIVNVAKVQDKPIMGVYMGGTVVERGRAILSGEHV
                     PVYNYPERAVRSMEAMVRYADYRRSLADGA"
BASE COUNT          394 a          778 c          760 g          358 t
ORIGIN      
        1 cgaccgccac gtccggcgca tcctcgcgta tgcgctccag acagcgcgcg aagatgtgcg
       61 cccctccatc gggaagatcg tcgcggttga cggaggtgat gacgacgtac cggaggccca
      121 ggatgcgaac cgaagccgcg agacgctcgg gctccgcctc gtcgaggccg ctcggtcgcc
      181 cgctcgtcac ggcgcagaag ccgcacgccc gcgtacacgt ctccccgagg atcatgaacg
      241 tcgcggtccc ccggccgaag cactcgccga tgttcgggca atgcgcctct tcgcacacgg
      301 tgtgaagatt gtgcttccgc acgagcgcct tgacgcgatg gaagttctcg ccggcgggga
      361 accggacctt gatccagtcg ggcttccgct cgcgccatgg agctgacgtc atagcaccgc
      421 ctcggctggc gccagaaggt cgacttcgaa tacgtcggag aagcgtcgcg ccaagcggtc
      481 ggcaacctcg tcgagcggcg gcggcgcacc gaggagtcgc gccatggagg tcacgcctgc
      541 atcggcgatg ccgcaaggca cgatgagctc gaagaacgat aggtcggggt ccacgttcaa
      601 cgcgaagccg tgcatcgtga cgccgcgact gactcgcacg ccgatcgccg ctatcttctc
      661 gtcgccaacc cagacgcccg cgcgacccgg tacgcagccc gcagcgatgc cgtactcttc
      721 caacgtgccg ctgatgacac gctcgagcag acggacgtac tgggggatgt ctgccgtgcc
      781 ccacagtctg cgcaagtcga ggatgggata gccgacgatc tggcccggcc cgtgaaacgt
      841 cacatctccg ccgcggtcga ctcgaaacaa acgcgcgcct cgggccagca gcatctcttc
      901 gcttgccaga acgtgcgcat cgtgcccgtt gcgaccgagc gtgaatgtgg gtggatgctg
      961 cagaaggagc agctggtgct ggccgcctgc ctggaccgca gccaggagtt cgtgctgctg
     1021 ccgccacgcc gcctcatagt cgacgaggcc ggcccgatgg atcaagcagc gctggcgcat
     1081 gtcgctctcc tcggccctac aacattcagc ctagcatggg gccgatgggg gaacaacgcg
     1141 gtcggcgctg gatttcgctt gaacaccgtg attctgccga gagagcgcga tcgctcacgc
     1201 gccgtcggcc agagagcggc ggtagtcggc gtagcggacc atcgcctcca tcgaccggac
     1261 ggctcgctcc gggtagttgt agaccggcac atgttccccg gaaaggatgg cccggcctcg
     1321 ctccacgaca gtgccgccca tgtagacgcc catgatcggc ttatcctgca ccttagccac
     1381 gttgacgatg atgcgcgccg tcgcctcggg ctccgtgacc gcctgcggcg tgaggagcgc
     1441 aatgatgccg tggaccgcct cgtcctcggc gatggcctcg agcgcgagcc gatatcggtc
     1501 ggcatcggcg tcgccgataa gatcgacggg gttgcggagg gacgcggccg caggcagacc
     1561 ttgccgcata cgttctatcg tttcgccgga gagccccgca agccttagtc cgagacgctc
     1621 tactgcgtct gcagccatca cccccggccc gcccgcgttg gtcacgatgg cgacgttcgg
     1681 ccccgctggg agcggcgcaa aggcgaagca tcgcgtgaaa tcgaagagct cctccatggt
     1741 gagggcgcgc acaattccgc tctgccgcag ggccgcatcg accacgcgct ccgatccggc
     1801 gagggcgccg gtgtgcgaga aggccgccgc cctcccgccc tgcgtcgtgc cgaccttcat
     1861 ggcgatgagg ggcttgcgtg cagtgaagtc tcgcgccgcg ttgaagaagg cgcggccatc
     1921 acggatgcct tcgaggtagg cgacggcgca ccgcacttcc ggatcggcgg tccaggcacg
     1981 tagcacatcg acctcggtga cgtcggccat gttgcccagc gatatgaact tcgagaagcc
     2041 gttccgtgtc tggaccgacc aatcgaggat ggccgacgcg acggcgcccg aatggctgaa
     2101 cacgcccacc tcccagggag gtggcaggcc atcggagaag gagacgttga ggccatgcag
     2161 cgcgtcgatc actccgagcg agttggggcc tacgagccgc atgccgtagc ggcgcgcaat
     2221 gtcgaggacg gcctgttcgc gctccacgcc ggcctcgccg gcctcgcgga acccggcgct
     2281 gatgatgacg
//
LOCUS       DATMBB010000025         4243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_553529, whole genome shotgun sequence.
ACCESSION   DATMBB010000025 DATMBB010000000
VERSION     DATMBB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553529"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VNL92_00490"
     CDS             <1..1117
                     /locus_tag="VNL92_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93222.1"
                     /translation="GLDEGFGYGNYEDEDFCARLQMLGLRIGVLPSTFVFHHGSVTFR
                     ENRIDHLEWMIRNLDRYVGKLERFCTQPPRDAVRRTVRADTAVSVIVRTHNRPDDLKI
                     ALNSLAWQSFDDFEVIVVNDGGCDVSEVLSSYEEHLRLEHVINESPRGPSAAANDGLA
                     AARGKYVAFLDDDDIAYPFHLASLVSCADRSQAPVVYSHYNRALVRGHGDEALVLERA
                     HMPPWRYDRSELLVQNRPAVHTWLVARHVFDRHGLFDADLTILWDWDFLLRVTEKSPL
                     VGLPRETCEYRIYLDLANSVTSSRRRALDELQRIYARHPASSGVVRLARKLEVAGLES
                     QVHLAARLLALADRGDITREDAARRYIAEVFGIRSIA"
     gene            complement(1114..2076)
                     /locus_tag="VNL92_00495"
     CDS             complement(1114..2076)
                     /locus_tag="VNL92_00495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93223.1"
                     /translation="MDLLSEMPGYRHSRVYLAPSPKPSLRRLATGVARAWWTGRTYQL
                     VHAHGEVAGGLCLPLLLARPSVVTLNGLHFLRRMSGLGRIAAVLNLRAVIRATDRMIC
                     VSRAEYEYLLAAVGPVAQRRAVVIHNGVRVPPPPSAAVRAEVRRELGLTNEEPVGIWV
                     GSLDARKDPLSAVRAAEAAALTLLVVGDGPLRAQVEQAAGAHVRVLGHRDDVGRLLQA
                     SDIYLMTSTREGLSFALLEAMACGLAPVVSDAPENLEAIGDASFAVTAGDSIDLAKSL
                     RSLAADSRRRRAAGHHAACRISRFFQASKMIHNTKEIYRAVGSF"
     gene            complement(2129..3271)
                     /locus_tag="VNL92_00500"
     CDS             complement(2129..3271)
                     /locus_tag="VNL92_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93224.1"
                     /translation="MQVCIVYDCLYPYTVGGAERWYRNIAERVAARGEEVTLLTLRQW
                     QGNVPSVPGVRVVSAGPRMSLYTSSGRRRLLPPVVFALGVLVHLLRHGRRYDVVHTAS
                     FPYFPLLVIAAARRACGFRVVVDWHEVWTRDYWRSYVGSLGGLIGWWVQGLCLRVRQR
                     AFCFSVLHERRLRELGLEAPLTRLEGQYDLDTEQYRPQPLEPVVLFAGRHIPEKRPAA
                     VVPAVVAAKTDIPDLRAEIFGDGPERSNVLAAIAELGAGSYVSAPGFVDGATLEKRLS
                     TALCVVLPSSREGYGRIVVEAAARGVPTVVVAGPDNAATELMEEGVNGTVAPSAGPDD
                     LAGAIVRIYRAGGELRDSTLEWFRRNAPRLSLEPSVAQILEAYASA"
     gene            3307..>4243
                     /locus_tag="VNL92_00505"
     CDS             3307..>4243
                     /locus_tag="VNL92_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWO93225.1"
                     /translation="MTSETVTGCQVSIVIPCLDEAETIADCVGAARAALAAAGLPGEV
                     IVVDNGSTDGSGALARAAGAVVIEEPRRGYGSAYLAGLAAARGDYLVMIDADLTYDFG
                     EIPRFVRALEDGADLVVGNRMRGVRPGAMPWLSRLGNPLLSGFLNVLHRTRVGDVHCG
                     LRAVRRSVLPLLNLRTVGMEFASEMVIRATREKLDVRELPIELHPRVGMSKLSPFRDG
                     WRHLRVILVYNPTFVFLVPGLIMLSTGTVLAVLVLLEVPVFGRALYIHSLIVGCLLVV
                     LGVQAIGLGLCARLFGVYFISEQDPLFQRLQSRFRL"
BASE COUNT          735 a         1417 c         1363 g          728 t
ORIGIN      
        1 tggcttggac gaaggatttg gttacggaaa ctacgaagat gaggactttt gtgctcgcct
       61 tcaaatgctt ggcttacgca ttggtgttct cccgagcaca tttgttttcc accatggaag
      121 cgtaacattt cgtgagaacc ggatcgatca cttggagtgg atgatcagga atcttgatcg
      181 ttacgtcggc aagctcgaga gattctgcac ccagccaccg cgtgatgctg tacgtcggac
      241 tgtgcgcgct gatactgctg tgagcgtgat tgttagaact cacaaccgcc cggatgactt
      301 gaagatcgca ctcaacagcc tcgcgtggca gagcttcgat gacttcgagg ttattgtcgt
      361 aaacgacggg ggctgtgacg tctccgaagt gctttcctca tatgaagagc acctacgact
      421 agaacacgtc ataaacgaga gtccgcgtgg accaagcgca gcagcgaacg acggactggc
      481 tgcagcccga ggtaagtacg tcgcctttct cgatgacgac gatatcgcct acccttttca
      541 tctggcctcg ttggtttcat gtgcagatcg atcgcaagct ccagtcgtct attcgcacta
      601 caaccgtgct ctcgtgcgag gacacggcga cgaggcgctc gttttggagc gcgctcacat
      661 gccgccctgg cggtacgaca gaagcgaact cttagttcag aaccggccgg ctgtccacac
      721 gtggctcgtt gcgagacacg tcttcgatcg ccacgggcta ttcgatgcgg atctcaccat
      781 cttgtgggac tgggacttcc tgcttcgcgt aacggagaag tcaccgttgg tcggcttgcc
      841 cagggagaca tgtgaatacc ggatctacct ggatctcgcg aactccgtga cctcgagtcg
      901 gcgccgggca ttggacgagc tccaacgcat atacgcgcgg caccccgcgt cttctggcgt
      961 cgtccggttg gcccggaagc tcgaggtggc cggacttgag tcacaagtcc acctcgcagc
     1021 caggcttctt gcactcgcgg accgcggtga catcacgcgc gaggatgcgg cgcgtcgtta
     1081 cattgctgag gtcttcggga tacggtcaat cgcctagaac gagccgacag cacggtagat
     1141 ctctttcgtg ttgtgaatca ttttcgacgc ttggaagaag cgtgagatac gacaggcagc
     1201 atggtggcca gccgctcgac gcctgcgcga atccgcagcg agactcctca gtgacttcgc
     1261 taaatcgatg ctgtcacccg ctgtcaccgc gaagcttgcg tcgccgatcg cctcgaggtt
     1321 ctccggcgca tcggacacca ctggcgcgag accgcacgcc atcgcctcga ggagtgcgaa
     1381 cgacaagccc tcccgagtcg atgtcatgag atagatatcg gaagcctgta ggaggcggcc
     1441 gacgtcgtca cggtgcccga gtacgcgcac gtgagctccg gccgcctgct ccacttgcgc
     1501 ccgcagcggg ccgtcaccga cgacgagcag cgtgagcgcc gccgcttccg cagcgcgaac
     1561 cgcggacagg ggatccttgc gggcgtcgag cgagccgacc cagatcccga ccggctcctc
     1621 atttgtgagg ccgagctcgc gacgcacctc ggcccgcacc gccgcgctcg gcggaggcgg
     1681 aacgcgaacg ccgttgtgga tgacaaccgc tcgccgctgg gcaacgggac caaccgcggc
     1741 caagaggtac tcgtactccg ctcgcgatac acagatcatg cgatccgtgg cacggatcac
     1801 tgcgcggagg ttcagcacag ctgcgatccg accgagaccg ctcatccgtc gcaggaagtg
     1861 cagcccgttc aacgtgacga ccgagggccg tgcaaggaga agcggaaggc acaacccgcc
     1921 cgccacctcc ccgtgcgcat ggaccagctg gtatgtgcgg cccgtccacc acgctcgggc
     1981 gacgcctgtt gccagccgac gcagcgacgg cttcgggctc ggcgcgaggt acacgcggct
     2041 atggcgatag cccggcatct cgctcaggag gtccacgtac gtctcgccgc caccgccggg
     2101 atgcggcagg acgtggagga ccgatctctc aggcggaggc atacgcctcg aggatctgcg
     2161 cgaccgacgg ctcgagcgag agccgcggtg cgttgcgtcg gaaccactcc agcgtggagt
     2221 cgcgcagctc cccgcctgct cggtagattc ggacgatcgc gccggcgaga tcgtccgggc
     2281 ctgcagacgg tgcgacggtt ccgttgacgc cctcctccat gagctcggtc gcggcgttgt
     2341 cgggacctgc gacaacgacc gtgggcaccc cgcgggcggc cgcctccacg acgatgcgac
     2401 cgtagccctc gcggctcgac ggcaggacga cgcagagagc agtcgacagg cgcttctcga
     2461 gcgtcgcccc gtccacgaac cccggcgccg agacgtagct gccggcgccg agctcggcga
     2521 tggcggccag gacgttggaa cgctcgggtc cgtcgccgaa gatctcggcg cgcagatccg
     2581 ggatgtccgt tttcgcagcc acgacagccg ggacgaccgc cgcgggccgc ttctcgggaa
     2641 tgtgacggcc ggcgaacaga acgacaggtt ccaggggttg cggccgatac tgctcggtgt
     2701 ccaggtcgta ctgcccctcg aggcgggtga gcggcgcttc gagccccagt tcacgtaggc
     2761 gccgctcgtg cagcaccgag aagcagaaag cccgttggcg gacacggagg cacaggccct
     2821 gaacccacca cccgatcaag ccaccgagcg acccgacgta cgagcgccag tagtcgcgcg
     2881 tccacacctc gtgccagtcc acgacgaccc ggaagccgca cgccctccgc gcggccgcga
     2941 tgacgagaag cgggaagtag ggaaacgagg ccgtgtgcac gacgtcgtac cggcgcccgt
     3001 ggcggagcag atgcacgagc actccgaggg cgaacaccac cggcggcaga aggcgccggc
     3061 gcccactgct cgtgtaaagg ctcatccgcg gccccgcgct cacgacccgt acgccgggga
     3121 cactcggcac gttgccctgc cactgccgga gcgtgagcag cgtgacctcc tcaccccgcg
     3181 cggcaacgcg ttcggcgatg ttgcggtacc agcgctcggc tccgcccacg gtgtacggat
     3241 agagacagtc gtagacgatg cagacctgca ccctccctat cctcccagcc tgaggtgact
     3301 gtggccgtga cgagcgagac ggtgaccggc tgccaggtct cgatcgtcat cccgtgcctg
     3361 gacgaggcgg agacgatcgc cgactgcgtg ggagccgccc gcgcggctct cgccgcagcc
     3421 ggcctgcccg gcgaggtgat cgtcgtcgac aacggctcga ccgacggaag cggtgcgctg
     3481 gcgcgggccg ccggcgccgt cgtcatcgag gagcctcgac gtggctacgg cagcgcgtac
     3541 ctggccggcc tcgcagccgc gcgcggcgac tacctcgtga tgatcgacgc ggacctgacg
     3601 tacgacttcg gcgagattcc gcgtttcgta cgcgcgctcg aggacggcgc cgacctcgtg
     3661 gtcgggaatc gcatgcgagg cgttcggccc ggggcgatgc cgtggctgag ccgcctcggg
     3721 aatcctctcc tctcggggtt cctgaacgtc ctccaccgca cccgggttgg cgacgtgcac
     3781 tgcgggctgc gtgcggttcg acgaagcgtg ctcccgctgc tcaacctgcg gacggtcgga
     3841 atggagttcg cctccgagat ggtcatccgc gcgacccggg agaagctcga cgtcagggag
     3901 ctcccgatcg agctccatcc gcgagtcggc atgtccaagc tctcgccgtt tcgagacggt
     3961 tggcgtcatc tccgcgtcat cctcgtctac aacccgacgt tcgtcttcct cgtgcccggg
     4021 ctcatcatgc tgtcaaccgg cacggtgctc gccgtgctcg tcctcctcga ggtgcccgtg
     4081 ttcgggcgcg ccctctacat ccactcgctg atcgtcggct gcctgctcgt cgtcctcggc
     4141 gtgcaggcaa tcgggctcgg gctctgcgcg cgactcttcg gcgtctactt catcagcgag
     4201 caggatccgc tgttccaacg gcttcagtct cgattccgcc tcg
//
LOCUS       DATMBB010000026         2157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_118979, whole genome shotgun sequence.
ACCESSION   DATMBB010000026 DATMBB010000000
VERSION     DATMBB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118979"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2157
                     /locus_tag="VNL92_00510"
     CDS             <1..>2157
                     /locus_tag="VNL92_00510"
                     /inference="COORDINATES: protein motif:HMM:NF037707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWO93226.1"
                     /translation="SLILLDEAHCSVPFLQTLRSIERYRGTGWAGSPPPCPFAFVVMS
                     ATPPADVPEDQVFPGADRDVALDHRLLRQRLHASKQAKLIELKTKGTEDPFVAEAASR
                     ARAFVEKGKQRIAVMVNRVATAFRVWDLLQQGSGEAYDVALLTGQLRPYERDQIVARW
                     TPFLKAREPDDLPRPLILVTTQCLEVGADYSFEALVTEAASLDALRQRFGRLDRMGVA
                     RRSPALVLIRDRDTDPAQSKPDPLYGSALAKTWELLKSKAAETSTIDFGVEALRTTLA
                     DIEELALYLAPTADAPVLLPAHLDLLCQTAPSAHPEPDVQQYLHGMGRGSPEVHIVWR
                     ADLGEDVGVWADVIALCRPVSAEMLSVPLWRVRQWLAGAATEDGTADVEGTQEVVGRE
                     RGGPLRPCLLWRGRARSKITRTPDDIAPGDVVIVPAAYGMEGLARPSGVDALGRHQLD
                     LWERALECAGRPAAVRLQHAVLEPWLRFEAIRRLVELAEAPSVEDEALQKAIDSALDL
                     SVVTEGDGPPPPSWWRDLLRTSRRGRIERHPAGGVILFECRTKAAAEEPDLFADDDDL
                     TCLGVGAQSLDEHSKLVRDTVEKLAARCLPEELRQVTTDAAYWHDCGKLDDRFQILLH
                     RGDELAALAAEEPLAKSTWMPASPTQRRALRDASGLPENFRHETLSVQLAQAHTPTIT
                     EQPLNDLFLHLIATHHGHARPLVPICDDPEPPSVHG"
BASE COUNT          393 a          707 c          672 g          385 t
ORIGIN      
        1 cagcctcatt ctgctcgacg aagcgcactg ttcggtaccg tttctccaga cccttcgttc
       61 catcgagcgc tatcgcggca cgggctgggc cggatcgccc ccgccctgtc cctttgcctt
      121 cgtggtgatg tcagccacgc cccccgccga tgtaccggag gaccaagtct ttcctggggc
      181 tgatcgtgac gtggcgctcg atcataggct tctccgccag cggctccatg ccagcaagca
      241 ggcgaagctc atcgagctga agacaaaggg gacggaagac cctttcgttg cagaggcggc
      301 ttcccgggcg cgagcgttcg tcgagaaggg caagcagcgg attgcggtga tggtcaaccg
      361 ggtcgcgacc gcctttcgag tatgggatct tctccaacag ggttctggtg aggcgtacga
      421 cgttgcgctg ctcaccgggc agctgcgacc ctatgaacga gatcaaatcg tcgcacggtg
      481 gaccccattt ctgaaagcac gggaaccgga tgacctgccc aggccgctca ttctggtcac
      541 tacgcagtgc ctggaagtcg gcgccgacta cagtttcgag gcgcttgtga ccgaagcggc
      601 aagtctcgac gctcttcgcc agaggttcgg ccgactcgac cgcatggggg ttgcgcggcg
      661 atcgcctgcc ctagtgctga ttcgagaccg cgacaccgac ccagcgcagt cgaaaccgga
      721 ccccctgtat gggtcggccc tcgcgaagac gtgggaactt ctcaaatcca aagccgcgga
      781 gacttcgacg atcgacttcg gcgtcgaggc gctccggacg accctcgctg atatcgagga
      841 actcgcactg tacctcgcac ccaccgcaga cgcccccgtc cttcttccgg cacacctcga
      901 cctcctctgc caaacagccc cctcggcgca ccccgaaccc gatgtgcagc aatatttgca
      961 cggcatggga cgtggctctc cggaagtcca cattgtgtgg cgggcggatc tcggcgagga
     1021 cgttggcgtg tgggccgatg tcattgcgct gtgccgtccg gtcagcgcag agatgctctc
     1081 cgttccgctc tggcgcgtgc gtcaatggct ggccggcgca gcgactgagg atggcactgc
     1141 cgacgtagaa ggcacgcagg aagtcgttgg gcgtgaacgc ggcggccctc ttcgcccatg
     1201 ccttctctgg cgtggtcgcg cgcgatcaaa aatcacgcgc acgcccgacg acatcgcacc
     1261 gggagacgtg gtgatagtgc cggcagcgta cgggatggaa ggcctggcgc ggccaagtgg
     1321 cgtcgatgcg ctcggccgtc accaactgga cctctgggag cgcgccttgg aatgcgctgg
     1381 tcgcccggct gccgtacgct tgcagcacgc ggtgctcgag ccctggctca ggttcgaggc
     1441 aattcgcagg ctcgtcgagc tagcggaggc accctcggtc gaggacgaag ctcttcagaa
     1501 ggccatcgac tccgcactcg acctttcggt cgtcacggaa ggagatggtc ctccgccccc
     1561 ttcatggtgg cgagatctcc tgcggacatc gcggagggga cgcatagagc ggcaccctgc
     1621 cggcggagtc attctctttg aatgcaggac gaaagccgcc gcggaggaac cggatctatt
     1681 tgcagatgac gatgatttga cgtgcctggg cgttggggcg caatcactcg acgagcactc
     1741 caagctcgtg cgggacacgg tggaaaagct cgcggcacgt tgtctgcccg aggagctacg
     1801 ccaggtcacg accgacgccg catactggca cgactgtggg aagctcgacg atcgattcca
     1861 gatattgctt catcggggcg acgaactcgc tgcgctggcg gcggaggaac cgctggcgaa
     1921 atcgacatgg atgcccgcct ccccaacgca acgtcgtgcg ctgcgggacg ccagcgggct
     1981 acccgagaac tttcgccacg agacgttgtc agttcagctc gcgcaggcac acactcccac
     2041 aatcacggag caaccgctca acgatctgtt tcttcacctc atcgccactc atcacggaca
     2101 cgctcgcccc ctcgtaccca tctgcgacga cccggagccg ccatcggtac acggcaa
//
LOCUS       DATMBB010000027         3125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_158509, whole genome shotgun sequence.
ACCESSION   DATMBB010000027 DATMBB010000000
VERSION     DATMBB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158509"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VNL92_00515"
     CDS             <1..741
                     /locus_tag="VNL92_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWO93227.1"
                     /translation="PPPETYKAIAAHLNEVIQDGDCIQVGVGEPSSLMPMLGAFDGKK
                     DLGLHTELGSPGMAKLVAEGVINGSRKNQFPKRAVAAAWTGCADDDLAIINDNPTFQL
                     FDPEYILDVRLMGRNDRQTAINSAISVDLIGQINAESVFGPRMINGTGGQPETHISAF
                     LSNGGKAITLLPSTALEGTVSRIVAMQDAGSIITIPRYYADIVISEYGVARLLGKNHR
                     ERAEELIAIAHPDFRAELRNSMKSMFFT"
     gene            788..1432
                     /locus_tag="VNL92_00520"
     CDS             788..1432
                     /locus_tag="VNL92_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWO93228.1"
                     /translation="MFEGLVEWVKDVIENLGYSGIVLIVTLENLFPPIPSEVVLPFAG
                     FLSGEGRFDYWLVVLAATAGSVLGAWILYGVGRFAGEQWVRDMLLRWGRYVLMSAHDL
                     ERADRWFADHGRPTVFFARLVPGVRSLVSIPAGFARQPLVEFTTYTLLGSATWNVILV
                     GAGHLLAENWDSVEGIVQPVSYVAYAALALAILYFAYRLYRRRPSRPSDDVVRR"
     gene            complement(1485..1826)
                     /locus_tag="VNL92_00525"
     CDS             complement(1485..1826)
                     /locus_tag="VNL92_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWO93229.1"
                     /translation="MQKIEAIIRPEKLDAVKVALADKGYIGLNIVGVTGRGVQRGIVH
                     AGRGAEPYSVDMLPKVKLETVVADANVEEVISIIQTAAQTGHIGDGKIFVIPVADAIR
                     VRTGERGDAAI"
     gene            complement(1842..>3125)
                     /locus_tag="VNL92_00530"
     CDS             complement(1842..>3125)
                     /locus_tag="VNL92_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWO93230.1"
                     /translation="FFYGGFVRNKNVLGTVMQSFIVMGIVGVLWVVMGYSLAFGPDVE
                     GWGIIGDLDHFGLKDVGADPDSQVPGIPHEAFMIFQAMFAIITPALITGAFAERAKFG
                     PFVIFMALWSLLVYAPVAHWVFDVEGWIASEVKAVDFAGGTAIHVNAGAAALAAALAF
                     GRRRGFKEEPMEPHDITYIVLGAAILWFGWFGFNAGSAGAANGLAANAFTVTNAAAAA
                     AALTWVGMSWWRTGKPSVVGAAAGAVAGLVAITPASGFVQPMEALAIGGIAGVVCYLA
                     VQVRLRTNVDDSLDVIAVHGIGGLWGALATGLFCVAAANTGTNALGEAVEGLTAGEGL
                     EQLGRQALGVLAVAPYSFVVTFIILKVLDLTLGIRVSDEDEVAGLDVSQHGERAYIFG
                     GAGPVLGIPEAIPIDTPLGEPLPASTLHGHHSVEP"
BASE COUNT          545 a         1077 c          955 g          548 t
ORIGIN      
        1 cctccgcccg agacgtacaa ggccatcgcg gcccacctga acgaggtcat ccaggacggc
       61 gattgcattc aggtgggcgt cggcgagccc tccagcctca tgccgatgct cggggcgttc
      121 gatggcaaga aggacctcgg cctccacacg gagctgggct cgcctgggat ggccaagctc
      181 gtcgcagagg gcgtcatcaa cggcagccgc aagaatcagt ttcccaagcg cgcggtggcc
      241 gcggcgtgga cgggctgcgc ggacgacgac ctcgccatca tcaacgacaa tccgacgttc
      301 cagctattcg atccggagta catcctggac gtccggctca tgggtcgcaa cgaccggcag
      361 accgccatca acagcgccat ctccgtcgac ctcatcggcc agatcaacgc cgagtccgtc
      421 ttcgggcctc ggatgatcaa tggcaccggc ggccagcccg agacgcatat cagcgccttc
      481 ctttccaatg gcggcaaggc catcaccctc ctaccgtcaa ccgcgctcga gggcaccgtc
      541 tcccgcatcg tcgccatgca ggacgcgggc tcgattatca ccatcccgcg ctactacgcc
      601 gacatcgtca tcagcgagta tggcgttgct cggcttcttg ggaagaacca ccgcgagcgc
      661 gcggaggagc tcatcgccat cgctcatccg gactttcggg cggagcttcg caactcgatg
      721 aagtcgatgt ttttcacata gataggggcg ctttgccgct actcggctct ccccgtatca
      781 tcgccatatg ttcgagggtc tcgtcgagtg ggtgaaggac gtcatcgaga acctcggcta
      841 ctcgggcatc gtcctcatcg tcacgctcga gaacctgttc ccgcccatac cgtcggaggt
      901 ggtgctgccc ttcgccggct tcctctcggg cgaagggcgg ttcgattatt ggctcgtcgt
      961 gctcgcagcc acagcgggct ccgtgctcgg cgcctggatc ctgtacggcg tcggccgctt
     1021 cgccggcgag cagtgggttc gcgatatgct gctgcgctgg ggacgctacg tcctcatgtc
     1081 ggcgcacgat ctcgagcgcg ccgaccgctg gttcgccgac cacgggcgcc ccaccgtgtt
     1141 cttcgcgcgg ctcgttcccg gcgtgcgaag cctcgtgtcg atacccgcag gattcgcgcg
     1201 tcagcctctc gtggagttca ccacctacac gctgctcgga agcgcgacgt ggaatgtcat
     1261 cctcgtcggc gccgggcacc tgctcgccga aaactgggac tccgtggagg gcatcgttca
     1321 gcctgtctca tacgtcgcgt atgcggcgct ggccctggcg atcctctatt tcgcgtatcg
     1381 cctctatcgc aggcggcctt cccgacctag cgacgacgta gtgcgtcgat agcgcgaggc
     1441 gcggaggggg gtgcgggcaa ctccacgcac cccctcgcgg ctgatcagat cgcggcgtcg
     1501 cctcgctcgc ctgttcggac gcgaatcgcg tcggcgacgg gaatgacgaa gatcttgccg
     1561 tcgccgatgt gtccagtttg ggccgccgtc tggatgatgc taatgacctc ctcgacgttc
     1621 gcatccgcca cgacggtctc cagcttgact ttcggcagca tgtccactga gtacggctcg
     1681 gcgccacggc ccgcatggac gatgcctcgt tgcacgccgc gtcccgtcac acccacgatg
     1741 ttgagcccga tgtagccctt gtctgccagg gcgaccttca cagcatccag cttctccggc
     1801 cgaatgatcg cttcgatctt ctgcacgtga cctctccctc gctatggctc gacgctgtga
     1861 tggccgtgaa gtgtggacgc aggcagaggc tcgccgagcg gcgtatcgat gggaatcgct
     1921 tcggggatgc ccaacaccgg ccccgcgcca ccgaagatgt acgctcgctc gccgtgctgc
     1981 gacacgtcca ggccggcgac ttcgtcctcg tcgctcacac gtatgcctaa cgtgaggtcg
     2041 agcaccttaa ggataatgaa tgtgacgacg aaggagtagg gcgcgacggc cagcacgccg
     2101 agcgcctggc gtccgagctg ctccagcccc tcccccgcgg tgagaccttc cacggcctcg
     2161 ccgagggcgt tcgtgcccgt attcgccgcc gcgacacaga acaagcccgt cgcaagtgct
     2221 ccccagagac cgcctatgcc gtgcaccgcg atgacgtcga gcgagtcatc gacgttcgtc
     2281 cgtaggcgga cttgcacggc aaggtagcac acgactccgg caatcccgcc gatggcgagc
     2341 gcctccatcg gctggacgaa acccgatgcg ggcgtgatgg cgacgagtcc ggctacggcg
     2401 cccgcagccg cgccgaccac gctcggcttg cccgtcctcc accagctcat gcccacccat
     2461 gtaagcgcgg cggcggccgc agcggcgttc gtcacggtga acgcgttggc ggcaagtccg
     2521 tttgcagcgc ccgcgctgcc cgcgttaaac ccgaaccacc cgaaccagag aatggcagcg
     2581 ccgagcacga tatacgtgat atcgtgcggc tccatgggtt cctccttgaa cccgcgacga
     2641 cggccgaacg ccaaggctgc cgcgagcgcg gcggctcctg cgttgacgtg gatcgcggtc
     2701 ccgcccgcga agtccactgc cttgacttcg gaggcgatcc agccctccac gtcgaaaacc
     2761 caatgcgcga cgggcgcata caccaggagc gaccacagcg ccatgaagat gacgaagggt
     2821 ccgaacttgg ctcgctcggc gaatgcgccg gtgatgaggg ccggggtgat gatggcgaac
     2881 atggcctgga agatcatgaa cgcctcgtgc ggaatgcccg gaacctgtga gtccgggtcc
     2941 gccccgacgt ccttcaggcc gaagtgatcc agatcgccga tgatgcccca gccctcgaca
     3001 tccggcccga acgcgagcga atagcccatc actacccaaa ggaccccaac gatacccatg
     3061 acgatgaagc tttgcatgac ggtgccaagg acgttcttgt tgcggacgaa gcccccatag
     3121 aagaa
//
LOCUS       DATMBB010000028         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_592952, whole genome shotgun sequence.
ACCESSION   DATMBB010000028 DATMBB010000000
VERSION     DATMBB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592952"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="hisG"
                     /locus_tag="VNL92_00535"
     CDS             <1..581
                     /gene="hisG"
                     /locus_tag="VNL92_00535"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWO93231.1"
                     /translation="RPSSSIAGLEVKVLRPQDMPQQVALENFDLAITGRDWLADHLYA
                     FPSSPVAEAVDLGRSRYSLAAVVPEDLPAEAIDGALALWRGREVRIASEYVNTADHYA
                     RLRRIPRYRVIPIHGASEGFVPDDADILIEGSETGTTLRANALKSIDNFFVSTSCVIR
                     STNPISGPRARLIDDIVARLARGAVAEAPRSR"
     gene            612..1346
                     /locus_tag="VNL92_00540"
     CDS             612..1346
                     /locus_tag="VNL92_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93232.1"
                     /translation="MILAKRAMERAGIASFDDLGAYYLGATTPDIRVLTRWERERTHF
                     FDLHCVEPQSSVDRLFEVHPELARPEQLSPSTVAFVAGYLSHLSMDEQYIQDVYRPHF
                     GVDSTLIDRARANLMDRVLQYELDLRQRADPTLVHHLQQELRASQLDIRVAFLDKETL
                     ERWREVSAEVAGQPVTWERFRHIAARHLRAAGIESDEELEAFIRDLPRLLDETISHVQ
                     AERIDEFLERSIDVTARAIARYLDCG"
     gene            1400..>2407
                     /gene="hisD"
                     /locus_tag="VNL92_00545"
     CDS             1400..>2407
                     /gene="hisD"
                     /locus_tag="VNL92_00545"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWO93233.1"
                     /translation="MDDLPPQVRDRLRAAFAEDLSPEEAVRRIIAGVRERGDDAVRRY
                     GDLLDGREGPAPERTRKLIVTRDEIDEAYGLVDESVVETLRRAAERIRTYHQRQRAHA
                     ATSFVKDGVGQLVSPLDRVGLYVPGTAVVYPSTVLMTALPARVAGTREVVMASPAGPD
                     GRVAPIKLVAADIAGVDWVVRAGGAQAIAALAYGTESIPSVDKICGPGNLFVTLAKRA
                     VFGDVGVDGVYGPSETIVIADDSALPELCAADLLAQAEHDELATPILLTTSATLAEAT
                     VAEVERQLRLIPRAEVARTALSARGGIAVLSSLEEAIALANDYAPEHLCLLVRDAEAL
                     LP"
BASE COUNT          387 a          832 c          798 g          390 t
ORIGIN      
        1 gccgcccttc ctcgagtatc gctgggctcg aggtgaaggt cctccggccg caggacatgc
       61 cgcagcaggt cgccctcgag aacttcgacc tcgcgatcac ggggagggac tggcttgccg
      121 accacctcta tgcgttcccc agcagcccgg tcgccgaggc cgtcgacctc ggacgctcgc
      181 gctacagcct ggccgccgtc gtccccgagg acctccctgc agaggcgatc gacggcgcgc
      241 tcgcgctgtg gcgagggcgg gaggtgcgca tcgcatcgga gtacgtgaac acggccgatc
      301 attacgctcg gctgcggcgt atcccccgct accgtgtcat accgattcac ggtgcctcgg
      361 agggattcgt tccggatgac gccgacatcc tcatcgaagg gtcggagacg gggacgaccc
      421 ttcgcgcgaa cgccctcaag tcgattgaca acttcttcgt ctcgacgagc tgcgtcattc
      481 gctccacgaa ccccatctcc ggaccccgcg cccggctcat cgacgacatc gtggcacgcc
      541 tcgcgagggg cgccgtagcc gaagcgccgc gttcacgcta agctaggccc atgccgcccc
      601 tcacgatgca catgattctc gccaagcggg cgatggaacg cgctggcatc gcttccttcg
      661 atgacctcgg cgcgtactac ctcggtgcca cgacacccga tatccgcgtc ctgacccgat
      721 gggagcggga gcggacccac ttcttcgacc tccactgtgt cgagccgcag agcagcgtcg
      781 accgcctttt cgaggtccat ccggagcttg ctcgaccgga gcagctcagc ccttcgacgg
      841 tcgcgttcgt cgccggatac ctgtcgcacc tcagcatgga cgagcagtac attcaggacg
      901 tctaccggcc gcacttcggc gtggattcga cgctcatcga tcgggctcgg gcaaacctca
      961 tggatcgcgt ccttcagtac gaactcgacc ttcggcaacg ggccgacccg acgctcgtcc
     1021 atcatctcca gcaggagctg cgcgcctctc aactcgacat aagagtggca ttcctcgaca
     1081 aggagaccct cgagcgctgg cgggaggtat cggccgaggt cgctggccag ccggtgacgt
     1141 gggagcgctt ccgccatatc gcggcgcgcc acctgcgcgc tgcaggcatt gagtcggacg
     1201 aggagttgga ggccttcatt cgagacttgc cgcgcctgct cgacgagacc atcagccacg
     1261 tccaggccga gcgtatcgac gagttcctcg agcgaagcat cgatgtgacg gctcgggcga
     1321 tcgcgaggta cctcgattgc ggatagtcca tgggtccgag gaggctcggc gctcgctgct
     1381 cgctcgtcgt cgcgagaccg tggacgacct tccgccgcag gttcgcgatc gccttcgtgc
     1441 tgccttcgcc gaggacctct cgcccgagga ggcggtgcgg cgcatcattg ccggcgtccg
     1501 tgagcgcggc gacgacgcgg tgcgccgcta cggcgatttg ctcgatggcc gcgaaggccc
     1561 ggcgccggag cgcacccgga agttgatcgt gacgcgcgac gagatcgacg aagcgtacgg
     1621 gctcgtcgac gaaagcgtcg tcgagacgct gcgacgagcc gccgaaagaa tacggaccta
     1681 ccaccagcgc cagcgggcgc acgcggcaac cagcttcgtc aaggacggcg tgggccagtt
     1741 ggtgagcccg ctcgatcgcg tcggcctcta tgtgcccgga accgcagtcg tctacccatc
     1801 gacggtgctg atgacggcgc ttcccgcccg cgtggcgggc acgcgggagg tggtgatggc
     1861 aagcccggcg ggccccgacg gacgcgtggc accgatcaag ctcgtcgcgg cggatatcgc
     1921 gggcgtcgac tgggttgtgc gggcgggggg agcgcaggcg atcgcagctc tggcctacgg
     1981 gacggagtcg ataccatccg tcgacaagat ctgcgggccg gggaacctgt tcgtcacgct
     2041 cgcgaagcgc gccgtcttcg gggacgtggg cgtcgacgga gtctatggcc ccagcgagac
     2101 gatcgtcatc gccgacgaca gtgccctgcc ggagctctgc gccgccgacc tgctcgctca
     2161 ggccgagcac gatgagctcg ccacgcccat cctcctaacc acgtcggcca ccctcgccga
     2221 ggcgaccgtc gcggaggtgg agcggcagtt gcgtctcatc ccgagggccg aagtcgcgcg
     2281 caccgcgctg agcgcgaggg gcggaatcgc tgtcctctcg tcgctcgagg aggccatcgc
     2341 actcgcgaac gactacgcgc cggagcacct gtgcctcctc gtgcgggacg cggaggcgtt
     2401 gcttccg
//
LOCUS       DATMBB010000029         6162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_158128, whole genome shotgun sequence.
ACCESSION   DATMBB010000029 DATMBB010000000
VERSION     DATMBB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158128"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VNL92_00550"
     CDS             complement(<1..221)
                     /locus_tag="VNL92_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWO93234.1"
                     /translation="MVLGGVLGALGVGLGAFGAHLVEDHVSSDDYDTYKTAAQYHLVH
                     ALALFAAAFAAERGKPSAALVAGRLFVAG"
     gene            complement(324..998)
                     /locus_tag="VNL92_00555"
     CDS             complement(324..998)
                     /locus_tag="VNL92_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWO93235.1"
                     /translation="MTLLQRVPLFPLNTVLFPRMTLPLRIFEERYKLMIGRCVEQELP
                     FGVVLIRSGVEVGGPAQPHEVGTLARIVHAHRNADGGFQIQTIGLQRFRIVALQDDEA
                     YLTADIEMLQYDRPAPGPEEDALRDLAQSVAVLYAEQLRLTLALSGMWQASLEAPDEP
                     EKLADLVAARITAPEIVKQRMLEAPTLMDRLELELATLTEIIPILTERVRQAQLVRWG
                     SLSAVN"
     gene            complement(1059..2672)
                     /locus_tag="VNL92_00560"
     CDS             complement(1059..2672)
                     /locus_tag="VNL92_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93236.1"
                     /translation="MIRTGRWPLLTVFAIGLIALASVWLTGIDSGNAPVGATGGTYVE
                     AVVGSADRANPLFASSPVERDLVKLLFAGLTRPGADGSILPDLAESWSVSEDGRRYTF
                     QLRPRLQWHDGEPITADDVVFTASLLSSPDYAGDPTIAALWRNVDARRIDRLTVEFEL
                     EQPFAPFLAATTIGLLPSHLLGDLSVQEVQASSFNQAPIGSGPFVLDSIGDDGARLRA
                     NRSYHLQPPYLDRLGLRFFETAEEAQLALQAGQVDGMLLPDRQAAEALSGDDFTIVKM
                     PTSAYVVLYLNHRSVLFVDSRVRQALALSIDREAIVADVLAGDGIASADPLAPGTWAS
                     TEEGGELEVDPDRAAELMEEAGWQLQDGTWRRGDIEMRFNILTNPDAERLAVAEAVAR
                     QLRDVGFLASVAEVDPNTLVQDFLQPRQYQAALFAFDPGIDPDPYPAWHSSQSGSGGA
                     NLASYVDEEADRLLAEARIATSEEARRQLYAALVARFREQAPSVILYYPERLYAVRDD
                     VRGVAPGAFFDRTSRFFAIEDWYRETRSP"
     gene            complement(2688..2921)
                     /gene="secG"
                     /locus_tag="VNL92_00565"
     CDS             complement(2688..2921)
                     /gene="secG"
                     /locus_tag="VNL92_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWO93237.1"
                     /translation="MEIEDFLNIAVILVALALIGTVLLQARGAGVGEMFGGGMGGSGY
                     RTRRGVERVLFQLTIVLMVVFVILAALNVRAHN"
     gene            complement(2980..4335)
                     /locus_tag="VNL92_00570"
     CDS             complement(2980..4335)
                     /locus_tag="VNL92_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HWO93238.1"
                     /translation="MIRALQAIQDRDFLKWLTPGLHIKRWLLLLMVGVAIMGLGIAYI
                     LREVYVSYTFPSGVYYLTLQFMPRIARGVLFMGAAVGLILFSTWKLNLSLLSAFMPAR
                     GREESLVNIIYNHRAAQRGPKIVAIGGGTGLSTLLRGLKQYTANLSAIVTVADDGGSS
                     GKLRREMGVLAPGDLRQCIAALAEAEPLMTELFQYRFSEGSGLEGHSFGNLFIVAMAE
                     VTGNFETALKETSRVLAVRGEIMPSTLSHVTLCATTEDHSMVYGESSITASGRRISEL
                     RLNPPHVRGYLGAIRAILEADLVVVGPGSLYTSVLPNLLVEDIRKALEITRARKLYVC
                     NVATQHGETDGFSVRDHLAVIDRHVGPGLFDAVLANSNLSAPIPSSAGAAPVNGKARG
                     EGPTAIGRARVLYADVVDHENGYRHDPAKLAEAIMSFYLDRRPVVHREVERPEAIAAS
                     V"
     gene            complement(4415..5905)
                     /gene="gatB"
                     /locus_tag="VNL92_00575"
     CDS             complement(4415..5905)
                     /gene="gatB"
                     /locus_tag="VNL92_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWO93239.1"
                     /translation="MTATTAVEYETVIGLEVHAQLLTKSKMFCGCSADYANAEPNTHV
                     CPICLGMPGVLPVINRAALEYTVMTGLALNCHIPLQAKFDRKNYPYPDLMKGYQISQY
                     DMPLCRDGFLLVEADGETRRVGVTRVHLEEDTARLLHRLDPETGEGYSLIDVNRSGVP
                     LMEIVSEPDIRSPEQARQYLMKLRQILRYLGVSAANMEEGNFRCDANVSLRPVGSSEL
                     GAKVEVKNMNSFRSVARALEYEVERQRAVLDSGGRIPQETRGWLELEGRTASQRSKEH
                     AHDYRYFPDPDLPPIVLTPEWISSIRERIPELPDAKRQRFVATYGLSDYEVGLLIETR
                     ARADYYEACVGPYRSEPARLPAMAKGCANWMLGEMARLQNLRGGSIEDAPVPPEHLRE
                     LVELIEAGALSSKMAKDVFAAVFETGKPPSVVVQERGLSQLSDEDELARLVDEIIAAN
                     PKPAADFRAGKQEALKFLVGQLMRATKGRAQPELANELLRKALSTQ"
     gene            complement(5902..>6162)
                     /locus_tag="VNL92_00580"
     CDS             complement(5902..>6162)
                     /locus_tag="VNL92_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93240.1"
                     /translation="DVAADAESVARALREPSDLVLVDLHMPDIDIEAIVQAAGETPVV
                     AFGRHTDAPALRAARQAGCAEVLPRSDFVTRLPALLSTEAPA"
BASE COUNT         1027 a         1951 c         2145 g         1039 t
ORIGIN      
        1 atgcccgcga cgaaaagccg ccccgccaca agagccgcgg atggcttgcc ccgctccgcg
       61 gcaaacgccg ccgcgaagag cgcgagcgcg tggacgagat ggtactgggc tgcggtctta
      121 tacgtgtcgt agtcgtcgga ggagacgtgg tcctcgacga ggtgcgcccc gaaggcgccg
      181 agccccaccc cgagtgcccc cagtacgcca ccgagtacca tcagtcgatg cgaggcgggg
      241 agcctcctgt actggcgcaa cagctgccgt acccgcgagg gtagggacgg gggtgaggtc
      301 ctgcggtcga gtgtggccat aagctaattc accgctgaga gcgagcccca ccgaaccagc
      361 tgggcttggc gcacgcgctc cgtgaggatg ggaatgatct cggtcagcgt cgccaattcg
      421 agttctagcc ggtccatcag cgtgggcgcc tcgagcatcc tttgcttgac gatctccggc
      481 gcggtgattc gggccgctac gaggtcggcc agcttctctg gctcgtcagg cgcctcgagg
      541 ctcgcctgcc acatgccgga cagcgccaac gtgaggcgaa gctgttccgc atagagcacg
      601 gcgaccgatt gcgccaggtc gcggagggcg tcctcctccg gtcctggagc gggcctatcg
      661 tattggagca tctcgatgtc ggcggtgagg taggcctcgt cgtcctggag cgcgacgatg
      721 cgaaaacgct gcaggccgat ggtctgaatc tggaagccgc cgtcggcgtt ccggtgggca
      781 tggacgatgc gcgccagcgt ccccacctca tggggctgcg caggaccgcc gacctcgacc
      841 ccgctgcgga tgagcaccac gccgaacggg agctcttgct cgacacaccg gccgatcatg
      901 agcttgtagc gctcctcgaa gatgcggagc ggcagcgtca tccgcgggaa cagcaccgtg
      961 ttcagcggga agagcggcac gcgctgcagc agcgtcatcg cctcaggcct cctctggtcc
     1021 gattatacgt cgagtctgct tatcgttcct ctcggacctc agggcgagcg cgtctcccga
     1081 taccaatcct cgattgcgaa gaagcgtgac gtgcgatcga agaaggcgcc gggcgcgact
     1141 ccgcgcacgt cgtcccggac ggcgtagagc cgctccgggt agtaaagaat gacgctgggg
     1201 gcctgctcgc gaaagcgggc caccaacgcg gcgtagagct gacggcgggc ctcctccgag
     1261 gtcgcgatgc gcgcctccgc caggaggcgg tccgcctcct catcgacgta ggaggcgagg
     1321 ttcgctccgc ccgagccgga ctgggagcta tgccaggctg ggtacgggtc ggggtcgatg
     1381 cccgggtcga aggcgaagag tgctgcctgg tactggcggg gctgcaggaa gtcctgcacg
     1441 agggtgttcg gatcgacctc ggcaacgctc gccaggaagc ccacgtcgcg cagctggcgc
     1501 gccaccgctt cggcgacggc aaggcgctcg gcatcggggt tcgtgaggat gttgaagcgc
     1561 atctcgatgt cgccgcgccg ccatgtacca tcctgcagct gccagcctgc ctcctccatg
     1621 agctccgcgg cgcggtccgg atccacttcg agttcgccgc cctcttcggt cgaggcccac
     1681 gttccgggcg cgagcgggtc ggccgaggcg ataccatcgc cggccagcac atctgcgacg
     1741 atggcctcgc ggtcgatact gagggcgagc gcctgccgaa ccctgctatc gacgaatagc
     1801 acggagcggt ggttgaggta cagcacgacg tacgcactgg tcggcatctt gacgatcgtg
     1861 aagtcgtcgc cagacagtgc ctcggccgcc tgccgatcgg ggaggagcat gccatcgacc
     1921 tgcccggctt ggagcgcaag ttgcgcttcc tccgccgtct cgaagaagcg caggccgagc
     1981 cggtcgaggt acggaggctg cagatgataa gagcgattgg cgcgcagcct ggcgccatcg
     2041 tcgccgatgc tgtccagcac gaagggtccg gacccgatgg gggcctgatt gaaggagctt
     2101 gcctgcacct cctgcacgga cagatcgccc agcaggtggc tcggcaatag cccgatcgtt
     2161 gtcgctgcca ggaacggagc gaacggctgc tcgagctcga attcaacggt aagccggtcg
     2221 atgcgccggg catcgacgtt gcgccagagg gcagcaatcg tcgggtcgcc ggcgtagtcc
     2281 ggggaggaca ggagcgacgc agtgaagaca acgtcgtccg ccgtgatggg ttcgccgtcg
     2341 tgccactgca ggcgaggacg gagctggaac gtatagcggc gtccgtcctc gctcacgctc
     2401 cacgattcgg cgaggtcggg caagatcgag ccgtcagccc ctgggcgcgt gaggccggcg
     2461 aacagcagct tcacgaggtc gcgctccacc ggagagctgg caaagagggg attggcgcgg
     2521 tcggcgcttc cgacgacggc ttcgacgtac gtgccgcccg ttgcgccgac gggcgcgttg
     2581 ccggaatcga tgccagtgag ccaaacgctg gcgagggcga tgaggccgat ggcgaagacg
     2641 gtcaggagcg gccagcgccc cgttcggatc atcacttcgg agctggccta attgtgggcg
     2701 cgaacgttga gggcggcgag tatcacgaag acgaccatga ggacgatggt gagctggaag
     2761 agcacacgct cgactccgcg tctggtgcga tatccgctgc cgcccatgcc cccgccgaac
     2821 atctcaccca cgcccgcacc ccgcgcttgg aggaggacgg tgccgataag cgcaagcgcc
     2881 acaaggatga cggcgatgtt gaggaagtct tcgatctcca tccgctctcc acggtagccg
     2941 atggggcgcg cttcgtctag tcgacggggc gccgcgcgac tatacgctcg ccgcgatcgc
     3001 ctccggccgc tcgacctctc ggtgcacgac gggcctgcga tcaaggtaga acgacatgat
     3061 cgcctcggcg agcttcgccg gatcgtgccg gtagccgttc tcgtggtcga cgacatcggc
     3121 gtagagaacg cgcgctcggc cgatcgcagt ggggccctct ccccgcgctt taccgttgac
     3181 cggagcggcc ccggcggagg aggggatggg cgccgagaga ttggagttcg ccagcactgc
     3241 atcgaacagg ccgggcccaa cgtgccggtc gatgacggca aggtggtcgc ggacagagaa
     3301 gccgtccgtc tcgccgtgct gtgtcgcgac gttgcagacg tagagcttgc gggcgcgcgt
     3361 gatttcgagc gccttgcgga tgtcctccac gagcaggttg ggcagcacgc tggtgtatag
     3421 gctgcccgga ccgacgacga cgagatccgc ctcgaggatc gcgcggatgg cgcccagata
     3481 gccccgaacg tgcggagggt ttagccgaag ctcgctgatc cgccgtccgc tcgccgtaat
     3541 cgacgactcg ccgtacacca tgctgtgatc ctcggtcgtc gcgcataacg tcacgtggga
     3601 gagggtcgag ggcatgattt cgccccgtac ggcgagaact cggctcgttt ccttgagggc
     3661 cgtctcgaag ttgcccgtga cctccgccat cgcgacgatg aacaggttgc cgaagctgtg
     3721 cccttcgagc ccgctgccct cgctgaatcg atactggaag agctccgtca tgagcggctc
     3781 ggcttcggcc agggcggcga tgcactgacg caggtcgccg ggcgcgagga ctcccatctc
     3841 gcggcgcagc ttcccggagg atccgccgtc atcggcgacc gtgacgatgg cactcaggtt
     3901 ggctgtgtat tgcttcagtc cgcggaggag cgtcgacagg cccgtaccgc ccccgatggc
     3961 aacgatcttc ggtccacgtt gcgcggcgcg gtggttatag atgatgttga cgaggctttc
     4021 ctcccgtccg cgcgccggca tgaaggcgga gagcagcgac aggttgagct tccaggtgga
     4081 aaagaggatg aggcccacgg ctgcgcccat gaacagcacg ccacgcgcga tgcggggcat
     4141 gaactgcaac gtgaggtagt acacgcccga tgggaaggtg tagctgacgt agacctcgcg
     4201 caggatgtac gcgatgccca ggcccataat cgccacgccg accatcaaca ggaggagcca
     4261 gcgtttgatg tgcaggcccg gcgttagcca cttgaggaag tcgcggtcct ggatggcctg
     4321 aagggctcgg atcatgttgc tgccgttctc cgaacggtta ccgcgccaca tctcaccgga
     4381 cgatagccgt ccggtccctt aatgtcaaac ggcctcattg ggtcgagagg gccttgcgaa
     4441 gcagctcgtt cgccagttcg ggctgggcac gtcccttcgt cgcgcgcatg agctggccga
     4501 cgaggaactt gagcgcttcc tgtttcccgg cacgaaagtc agcggcaggc ttgggattcg
     4561 cggcgatgat ctcgtcgacg aggcgggcaa gctcgtcctc gtcgctgagc tgggagaggc
     4621 cgcgttcctg cacgacgacg ctcggcggct tccccgtctc gaatacggct gcgaagacat
     4681 ctttcgccat cttgctgctc agggcgcccg cctcgatgag ctccaccagc tcgcgcaggt
     4741 gctcgggcgg gaccggcgca tcctcgatgc tgccgccgcg taggttctgc agacgcgcca
     4801 tctcgcccag catccagttc gcgcagccct tcgccatcgc gggcaggcgg gcgggctcgc
     4861 tgcggtacgg cccgacgcag gcctcgtagt agtcggcccg ggcgcgcgtc tcgatgagga
     4921 ggccgacctc gtagtctgac aggccgtagg tcgccacgaa tcgctgccgc ttggcgtccg
     4981 gcagttccgg gatgcgctcc cgaatcgagc tgatccactc gggcgtgagg acgatgggcg
     5041 ggaggtctgg gtcggggaag tagcgatagt cgtgggcatg ctccttcgag cgttgcgacg
     5101 cggtgcggcc ttccaactcg agccagccgc gcgtctcctg cgggatgcga ccccctgaat
     5161 cgaggacggc gcgctggcgt tcgacctcgt actcgagggc tcgggcgacg ctacgaaagc
     5221 tgttcatgtt cttgacttcg acctttgctc cgagctcgga cgagccgacc ggtcgcaggc
     5281 tcacgttggc atcgcaacgg aagttgcctt cctccatgtt ggccgcgctc acgcccaggt
     5341 agcggaggat ctggcgaagc ttcatcaggt actggcgcgc ctgctccggg gagcggatgt
     5401 cgggctcgga gacgatctcc atgagtggaa cgcccgagcg gttcacgtcg atgaggctgt
     5461 acccctcacc tgtttcggga tcgagccggt gcaagaggcg cgccgtatcc tcctcgaggt
     5521 gaacccgtgt cacgccgacc cgccgcgttt cgccgtcggc ctcgaccaac aggaatccat
     5581 cgcggcagag cggcatgtcg tactgcgata tctggtagcc cttcatcagg tcggggtacg
     5641 ggtagttctt tcgatcgaac ttggcctgca gcggaatgtg gcagttgagg gcaaggcccg
     5701 tcattaccgt gtattcgagc gcggcgcggt tgatgacggg caggacgcca ggcatgccca
     5761 ggcatatggg gcagacgtgg gtgttcggtt ccgcgttcgc gtagtcggcg ctgcagccgc
     5821 agaacatctt gcttttcgtg agcagctggg catggacctc gaggccgatg acggtctcgt
     5881 actcgacggc ggtggtggcg gtcatgcggg cgcctccgtg gagaggaggg cggggagcct
     5941 cgtcacgaaa tccgagcggg gcaacacttc ggcgcagccg gcctggcgag cggcgcgcag
     6001 ggcaggcgca tccgtatgcc gcccgaaggc aacgaccggt gtctcgcccg cggcctggac
     6061 gatggcctcg atgtcgatgt ccggcatatg caggtcgacg agcacgaggt cgctgggctc
     6121 gcggagggcg cgggcaacgg actcggcatc cgcggcgacg tc
//
LOCUS       DATMBB010000030         5298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_552641, whole genome shotgun sequence.
ACCESSION   DATMBB010000030 DATMBB010000000
VERSION     DATMBB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552641"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VNL92_00585"
     CDS             complement(<1..912)
                     /locus_tag="VNL92_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93241.1"
                     /translation="MDMGKSFGETARLWARWVAVLPAAVLSVLLVSFPIHWAVLAITT
                     GANDDGVLGITDLQPETLERLVIAFFGPMAYVVVGAKVAPKHRLRTAVVLIVLLAIVL
                     SASTTYVATSDRFDYDYEGGGWAEFVAVVGLWIGGVLAGIYYINENPSLDARGRDIRG
                     SVKRIGPSLAIALICVVGLAGSALYIGIAGEDQAEPGWAYWKEDLARRQLEVDELFLN
                     GSMTKKEWVEAYSGLQLERWVRAKDMYGSGESGVVDRYFAKLEELSERSPGGVITLEA
                     WDELEAWIAALPPEDQKRLDDDLGVGGT"
     gene            complement(1079..1810)
                     /locus_tag="VNL92_00590"
     CDS             complement(1079..1810)
                     /locus_tag="VNL92_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93242.1"
                     /translation="MRGLAAALALVQFVLGAWLLTSGWDEGNASLPVIAGVAVVVGAV
                     STATGIAVARRHRRLAATMYLAGGLLGLPLVATLVYAPVPVLQIIFARHLVEHLRSGD
                     DTDRRAGGHASAAGRVAIVLACLQILLGLLSIWNGIRDDPAPLTIFDYVRDDESDLHS
                     SWPAVSIGLAIAGGGLLAIIGLYLAEREPQAGALLIALGALLGLGLVFFLIIVPLLLA
                     AFGLSAAGAKDPALPVSRNSRLQSR"
     gene            complement(1881..2291)
                     /locus_tag="VNL92_00595"
     CDS             complement(1881..2291)
                     /locus_tag="VNL92_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWO93243.1"
                     /translation="MRFLDANVFLRYLTRDDERKAEACRALFRRLQRGEEEATTNEVI
                     LHEVLYVLCSPRLYELTHEEAAARLRPILAVRGLRLPHKRRYQRALDLFATFPFLDFG
                     DALAIAQMDDQGMTELYSYDADFDRVEAVKRVEP"
     gene            complement(2288..2560)
                     /locus_tag="VNL92_00600"
     CDS             complement(2288..2560)
                     /locus_tag="VNL92_00600"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWO93244.1"
                     /translation="MKKILSTVTSRGQVTIPAEVRKVLGAKSGDKVAFEIVGDSVMLK
                     RARFTLETAYGSVPPKHRPEDFEAMKDQAMEEHAEEVARVLHRRRR"
     gene            complement(2698..3141)
                     /locus_tag="VNL92_00605"
     CDS             complement(2698..3141)
                     /locus_tag="VNL92_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWO93245.1"
                     /translation="MQRPDLIETFKHLGIRVTAQRLAVARVLADSKDHPTAQDVFERV
                     RQHLPHISLATVYNTVAVLENKGLVQALVFADGKRYDTNIMSHANLVCVECGRIVDVD
                     DEDGVVARLRDKVEAASGFDVIGQRLDFYGACSECRPLPAGRGRD"
     gene            3403..>5298
                     /gene="katG"
                     /locus_tag="VNL92_00610"
     CDS             3403..>5298
                     /gene="katG"
                     /locus_tag="VNL92_00610"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HWO93246.1"
                     /translation="MSENGSESENPGIPSPTPKQTRPRTNRDWWPNQVDLSILHQPSL
                     LADPMGADFNYAEEFKQLDLEALKRDLIEVMTTSQDWWPADYGHYGPLFIRMTWHAAG
                     TYRIADGRGGGGSGAQRFAPLNSWPDNANLDKARRLLWPIKQKYGRKLSWADLLVYAG
                     NVAMESMGFKTFGFGFGREDIWEPEEIFWGPEDTWLGDERHASGGEMVGPFGADHMGL
                     IYVNPEGPNGNPDPAAAAKFIRQTFARMAMNDEETVALIVGGHTFGKAHGTVSAEYIG
                     PEPEAAPIEEQGLGWRNRFGSGKGPDTMTSGLEGPWTTEPTKWDNGFLENLLKYDYEL
                     TTSPAGGKQYAPKPNGSAVPTAPDAHDPSKRQTTMMLTTDLALKVDPIYAPIVQRFYE
                     HPDQLADAFARAWYKLLHRDMGPVSRYLGPWVPEPQLWQDPVPPVDHELIGEEDIAVL
                     KSKILASGLSISRLVYTAWSAAASFRGTDKRGGANGARIRLAPQKDWEVNEPGELTKA
                     LRTLEQIQAEFNNAQTGRKKVSLADLIVLAGCAAVEQAARDAGHDVTVPFAPGRTDAP
                     QEQTDVESFAVLEPTYDGFRNYLRAGEKLSPETLLVERANMLTLTAPEMAVLVAGMRA
                     LNANARQA"
BASE COUNT         1062 a         1731 c         1629 g          876 t
ORIGIN      
        1 cgttccgcct acgcccaagt catcatcgag ccgtttctga tcctcaggcg gcagcgcagc
       61 tatccacgcc tccagttcgt cccacgcctc tagggtgatg acacctcctg ggctccgttc
      121 gctcagctcc tcaagcttgg caaagtaccg atctaccacc cccgactcgc ccgaaccgta
      181 catgtccttt gcccgtaccc accgttccaa ctgaaggcca gaatatgcct ccacccactc
      241 cttcttcgtc atggagccgt tcagaaatag ctcgtcaact tccaactgcc tccgggcaag
      301 gtcctctttc cagtacgccc accctggttc cgcctggtcc tctccggcga tgccaatgta
      361 gagcgcgctt ccagcgagac ctaccacgca gatcagcgcg atagcgagcg aggggccaat
      421 gcgcttcacg gatccacgta tgtcgcgccc gcgcgcatct aaagatgggt tctcgttgat
      481 gtagtagatg ccggccagga ctccgccaat ccacagcccg acgaccgcaa cgaactcagc
      541 ccagccccct ccttcgtagt cgtagtcgaa cctgtcactc gtcgccacgt aggtcgtact
      601 cgccgacagc acgatcgcga gcagaacgat gaggacgacg gcagtccgga gtctgtgctt
      661 cggtgcgacc ttggcgccaa cgaccacata ggccatcggg ccgaagaatg ctattaccag
      721 ccgttctagc gtctccggct gcagatcggt gatgccgaga acaccatcgt cgtttgcgcc
      781 ggtcgtgatg gccagaaccg cccaatgaat cgggaagctg accaagagta cggacagcac
      841 tgccgcagga aggacagcga cccagcgtgc ccataatctc gctgtctcac caaaggactt
      901 gcccatatcc accctgatgc ctcgtgacac tatcgcgagt agtcgccgac cgaaggcatt
      961 atatgggcaa tttcgtgagg tcccctctag tcttgctgac gccgcgctat ggcgggagct
     1021 aggcgatgcc cgcattccat gcatatcacg ttcgcgtgcg acatgatgtt ggtgtcgttc
     1081 agcgagactg cagacgggag tttcgtgaga caggcaaggc ggggtccttc gctccggcag
     1141 ccgacagccc gaatgccgcg agcaagagcg gtacgattat gaggaaaaag acaagtccga
     1201 gaccaagcag cgcgccgagg gcgatgagca gcgccccggc ttgaggttcc cgctcggcca
     1261 ggtagagacc gatgatcgcc agaagtcccc cgcccgcgat cgcgaggccg atggacacag
     1321 caggccagga cgagtgcaga tcggactcgt cgtcgcggac gtagtcgaag atcgtcagcg
     1381 gcgcgggatc gtctcggatt ccattccaga tcgagagcag gccaagcagg atctggaggc
     1441 atgccagaac gatggcgacc cgtccggccg cggaggcatg gcccccggcc cggcggtccg
     1501 tatcatcgcc gctgcgaaga tgctcgacga gatggcgggc aaagatgatc tgtaggacgg
     1561 ggaccggagc ataaacgagc gtagcgacca gtgggagtcc gagaaggccg cctgccaagt
     1621 acatcgtggc cgcgaggcgt cgatgccggc gtgccacggc gatgccggtc gccgtgctca
     1681 ccgcgccgac gacaacggcc acgcccgcaa tgacggggag cgatgcattc ccttcgtccc
     1741 agcccgaggt cagcagccac gccccgagta cgaactgcac cagggccagc gccgccgcca
     1801 agccgcgcat gatccgattc caaacccgac tcatgcgttc atgcctgcac ggagacccca
     1861 tgctcctgcc tgcatcgctt tcatggctcg acccgtttca ccgcctcaac ccgatcgaag
     1921 tccgcatcgt acgagtacag ctccgtcatg ccctggtcgt ccatttgcgc tatcgctagg
     1981 gcatcgccaa agtcgagaaa cgggaacgtc gcgaataagt ccagggcacg ttggtagcgc
     2041 ctcttgtgcg gcaagcggag accgcggacg gcaagaatcg gcctcaaccg cgcggccgcc
     2101 tcctcgtggg tcaactcgta cagacgtggt gaacaaagca cgtagaggac ctcgtgaagg
     2161 atcacctcgt tagtggtagc ttcctcttcg ccgcgctgaa gtcgccggaa cagcgcccga
     2221 caagcctccg cctttcgttc atcatcccgc gtcagataac gcaggaacac gttggcgtcc
     2281 aggaatctca tcggcgccta cggtgcagaa cgcgcgcaac ctcctccgca tgctcctcca
     2341 tcgcctggtc cttcatggcc tcgaagtcct cggggcgatg cttgggtggc accgagccat
     2401 aggccgtctc aagcgtgaag cgagcccgtt tgagcatcac agaatcgcct actatctcaa
     2461 aggcaacctt gtccccactc ttggcgccca gaaccttgcg gacctccgct ggaatcgtga
     2521 cctggcctcg gctcgtaacc gtcgagagaa ttttcttcat ttcaatcgcc gcctgtcact
     2581 gctaaatatg taacgaagta tagcaagtgc attgatcatt ctcaaggaca acgcagccca
     2641 ataggacaac gcagcccaat tgggccccgt ctggcgagcc acaggagatg ctggaggcta
     2701 atctcggcct ctgcccgcag ggagggggcg gcattcggag caggcgccgt agaaatcgag
     2761 ccgctggccg atgacgtcga agccgctggc tgcctcgacc ttgtcgcgca gacgcgcgac
     2821 gacgccgtcc tcgtcgtcca cgtcgacgat ccggccgcac tccacgcaga ccaggttggc
     2881 atgcgacatg atgttcgtgt cataacgctt gccatcggcg aagacgagcg cctgcacgag
     2941 gcccttgttc tcgagcacgg caacggtgtt gtagaccgtg gcgagcgaga tgtgcggaag
     3001 atgctgccgc acccgctcga agacatcctg tgcggtcggg tggtccttcg agtcggcgag
     3061 gacacgcgcc acagccaagc gctgggccgt cacccggatg ccgaggtgct tgaacgtctc
     3121 gatgaggtca ggtcgttgca tggcgtgtaa accgattcta ggtcgcgcgc cttggccaag
     3181 caaatttctt gtaccggggc tcatttcgcc ggttgggcgc ctagactcgt cctttagaac
     3241 tgtcctaaga tacaccggca tggaagggcg cccacgtccg cgaaggccat ggaaggtcaa
     3301 cgggcggcac cggcgatgaa ccaaacaatg ggctgttcag ccggctgcgt cgcccgagcc
     3361 atgggacatc acaaacgaaa cacgtgagag gagaggaccg acgtgtctga gaacggcagc
     3421 gagagcgaga atccaggaat cccatcaccg actcccaagc agactcgtcc caggaccaac
     3481 agggactggt ggccaaatca ggtcgaccta tcgattctcc accagccctc cctgctggcg
     3541 gatcccatgg gtgcggactt caattacgcg gaggagttca agcagctcga tctcgaggcg
     3601 ctgaagcgag acctcatcga ggtcatgacg acatcgcagg actggtggcc ggccgactac
     3661 ggtcactatg ggccgctctt catccggatg acgtggcacg ccgcgggcac gtaccgcatc
     3721 gccgatgggc gcggaggtgg cggcagcggc gcccagcgct tcgcccctct caacagctgg
     3781 cccgataacg cgaacctcga caaggcgcgc cgtcttctct ggcccatcaa gcagaagtac
     3841 ggccgaaagc tctcctgggc cgacctgttg gtctacgccg gcaatgtcgc catggaatcg
     3901 atggggttca agacgttcgg attcggcttc gggcgagagg acatctggga acccgaggag
     3961 atcttctggg gccccgagga cacgtggctt ggcgacgagc gccacgccag cggcggcgag
     4021 atggtggggc cgttcggcgc cgaccacatg ggcctgatct atgtgaatcc ggaggggccg
     4081 aacggcaacc ccgacccggc cgctgcggca aaattcatcc gacaaacgtt cgcgcgcatg
     4141 gccatgaacg atgaggagac ggtcgcgctc atcgtcggcg gccacacgtt cggcaaggcc
     4201 cacggcacag tgagcgcgga gtatatcggt cccgagccgg aagctgctcc catcgaggag
     4261 cagggtctcg gctggcgaaa ccgattcggc tctgggaagg gtccggacac gatgacgagt
     4321 ggtctcgagg gcccgtggac gacggagccg acgaagtggg acaacggctt tctcgagaac
     4381 ctgctcaagt acgactacga gctgacgacc agccccgccg gcgggaagca gtacgctccc
     4441 aagcccaacg gatcggccgt acccaccgca cccgatgccc acgatccgtc gaagcggcag
     4501 acgacgatga tgctgacgac ggacctcgcg ctgaaggtgg acccgatcta cgcgccaatc
     4561 gtgcagcgct tctacgagca tcccgaccag ctggcggacg cgttcgcgag ggcgtggtac
     4621 aagctgctgc accgcgacat gggccccgtc tcgcgctacc tgggcccgtg ggttcccgag
     4681 ccccagcttt ggcaggaccc cgttccgccc gtcgaccacg agttgatcgg cgaagaggac
     4741 atcgctgtgc tcaagagcaa gatcctcgcc tcggggttgt ccatctcgcg gctggtctac
     4801 accgcctggt cggcggcggc cagcttccgc ggcacggata agcggggcgg ggccaacgga
     4861 gcgcgtatcc ggctcgcgcc ccagaaggac tgggaggtca acgagcctgg cgagttgacc
     4921 aaggcactgc ggaccctcga gcagatacag gcggagttca acaacgcaca gaccggacgc
     4981 aagaaggtct cgctcgcgga cctcatcgtt ttggccgggt gcgcggccgt cgagcaggcg
     5041 gcgagggacg ccggccacga cgtcaccgtc cccttcgcgc ccgggcgcac cgacgccccc
     5101 caggagcaga ccgacgtcga gtcgttcgcc gtgctcgaac cgacctacga cgggttccgc
     5161 aactacctca gggcgggcga gaagctgtcc cccgagacgc tgctggtcga gcgggccaac
     5221 atgctcacgc tcacggcgcc ggagatggcg gtgctggtgg ccggcatgcg ggccctcaac
     5281 gccaacgcga ggcaggcg
//
LOCUS       DATMBB010000031         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_192591, whole genome shotgun sequence.
ACCESSION   DATMBB010000031 DATMBB010000000
VERSION     DATMBB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192591"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1179
                     /locus_tag="VNL92_00615"
     CDS             232..1179
                     /locus_tag="VNL92_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93247.1"
                     /translation="MTKYIIRRVLLAIPTILLVMFLVFMMLRLVPGDLVEIMLAERPY
                     STPEDRAALEEQLGLDEPIPIQFVRYVGNVLRGDLGESPWTTTPVTTEIGNRLPVTLE
                     FGLMAILFGLMISLPVGIVSAIRQDSIADYASRSFAILALSVPYFFTSILIIVFGPKW
                     GWTPPLLYKGWSDGPGAHLYYFVTPALLLGISLAGCVMRLTRTMMLEVLRQDYIRTAW
                     AKGLRERGVIMRHALKNAFIPVITIVGLQVGTAISGTIILETIFNMPGMGRYFVGAIL
                     QRDYPSVQGVVLVLASVVVFVNLLVDLTYGWLDPRIRYS"
     gene            1197..>2150
                     /locus_tag="VNL92_00620"
     CDS             1197..>2150
                     /locus_tag="VNL92_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93248.1"
                     /translation="MAQQQAAGAPAIEAGPEQVRGRRLPFYVDVLRRLVKEKPLGLFG
                     GILVLVIALAAVFAGIIAPYGPNALGVGESLAGPSWDHLFGTDNLQRDVFSRVIHGAR
                     VSMWIGLLAVITSTMLSMSIGVTSGYFGGWLDMVAQRVVDAFIAFPGLIFLLAVIALF
                     RDANMPGLPREGLLSTQIIILIITIGILLGVGSSRVIRSATLSVKSTTYVEAARAIGA
                     GHGRMIFVHVLPNIMAPVITLATLGFGTAILLEASLSFLGFGVPPNIPSWGGMLNREA
                     RSYMTEAPWLALAPGIALSLAVFGFNMLGDALRDILDPRMRG"
BASE COUNT          329 a          752 c          650 g          419 t
ORIGIN      
        1 gtgcaggaag cgcagcggat gatcgtgctg gacttcccca tgaagttcat gttcacgacg
       61 aacaaccacc tcttcaccga ccagaaggtg aagggctggt tctacccgac ggacctgtat
      121 gacggccgca aggccgaggc ctggctcgac gcctgagcgg ggcatagacg gacggcggcg
      181 gggctggcat agtccagccc cgctgctgca caatcgccgc gaggatagga gatgaccaag
      241 tacatcatcc gccgggtgct cctggcgatc ccgacgatcc tgctcgtgat gttcctggtc
      301 ttcatgatgc tccgcctcgt gcccggggac ctcgtcgaga tcatgctcgc cgagcggcca
      361 tactcgacac ccgaggaccg cgccgcactc gaagagcagc ttggcctcga tgaaccgatt
      421 cccatccagt ttgtccgcta cgtcggcaac gttttgcgtg gcgacctcgg cgaatcgccg
      481 tggacgacca cgcccgtgac cacagaaatc ggcaaccgcc tcccggtcac gctcgaattc
      541 ggcctgatgg ccatcctctt cggactcatg atctcgctgc ccgtgggcat cgtctcggcc
      601 atccggcagg acagcatcgc ggactatgcg tcgcgaagct tcgccatcct cgcgctctcg
      661 gtgccgtact tctttacgtc gatcctgatc atcgtcttcg ggccaaagtg gggttggacg
      721 ccgcccttgc tctataaagg ctggagcgac ggcccgggcg cccacctcta ctacttcgtg
      781 acgccggccc tgttgctcgg catctcgctt gccggctgtg tgatgcgcct gacgcggacg
      841 atgatgctcg aagtccttcg ccaggattac atccggacgg cctgggcgaa ggggctccgc
      901 gagcgcggcg tgatcatgcg ccacgccctc aagaacgcct tcatccccgt gatcacgatc
      961 gtcggcctcc aggtgggcac agccatcagc ggcaccatca tcctggagac gatcttcaac
     1021 atgcccggta tgggccgcta tttcgtgggc gccattctcc agcgcgacta tccgagcgtg
     1081 cagggcgtcg tccttgtcct ggcctcggtt gttgtcttcg tcaacctgct ggtcgacctc
     1141 acctatgggt ggctcgaccc gcgcatccgc tactcctgag gtctggaggg ctcaccatgg
     1201 cacagcaaca ggccgctggc gcacccgcaa tcgaagccgg ccccgaacag gttcgcgggc
     1261 ggcggctgcc cttctacgtc gacgtcctcc gcaggctggt gaaggagaag ccgcttgggc
     1321 tcttcggcgg catcctcgtg ctcgtcatcg cgcttgccgc ggtcttcgcg ggcatcatcg
     1381 ccccctatgg ccccaacgcc ctcggagtgg gcgagtcgct cgccgggccg tcctgggacc
     1441 acctgtttgg gaccgacaac ctgcaacgtg acgtgttcag tcgcgtgatt cacggcgccc
     1501 gcgtctcgat gtggatcggc ctgctcgcag tcatcacctc caccatgctg tcgatgtcga
     1561 tcggcgtcac cagcggctac ttcggcggct ggctcgacat ggtcgcgcag cgcgtcgtcg
     1621 atgcgttcat cgcattcccg ggcctcatct tcctgctcgc ggtcatcgcg ctctttcgcg
     1681 acgcgaacat gccgggcctc ccgcgggagg gcctcctcag cacgcagatc atcatcctga
     1741 tcatcacgat cggcatcctc cttggcgtgg ggtcatcgcg cgtgatccgg agcgctaccc
     1801 tcagcgtgaa gagcacgacc tacgtcgagg ccgcgcgcgc cattggcgcc gggcacggcc
     1861 ggatgatctt cgtccacgtg ctgccgaaca tcatggcgcc ggtgatcacc ctggcgacgc
     1921 tcggcttcgg cacggccatc cttctcgagg cctcgctgag cttcctgggc tttggtgtgc
     1981 cgccgaacat cccgtcctgg ggcggcatgc tgaaccgcga ggcacgctcc tacatgaccg
     2041 aggcgccctg gctcgcgctc gcccccggga tcgcgctgag cctcgcagtc ttcggcttca
     2101 acatgctcgg cgacgcgctc cgcgacatcc tcgacccgcg tatgcgtggc
//
LOCUS       DATMBB010000032         4835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_117585, whole genome shotgun sequence.
ACCESSION   DATMBB010000032 DATMBB010000000
VERSION     DATMBB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117585"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..574)
                     /locus_tag="VNL92_00625"
     CDS             complement(20..574)
                     /locus_tag="VNL92_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93249.1"
                     /translation="MIDRLPGYEFTTGEARRSVVRSAIIFTPLLLLTLAVLIAMIVNR
                     ATGGDASLVLIVIVGLIALLVGFQSIEALRDLRAELKETRGRVRRSWSRSDFFFWRSY
                     YIHVDETVFKVRPETHVVLKEGDEVVITHMPHTATIETIAKVRPEPSPPETGPSPATD
                     DDDEPPAHRSESSDPQAPTVRQND"
     gene            complement(571..1050)
                     /locus_tag="VNL92_00630"
     CDS             complement(571..1050)
                     /locus_tag="VNL92_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWO93250.1"
                     /translation="MTTCYACASTATASCPRCGTAYCSEHGGEICARCANPAAGPSQQ
                     WYRGSLLAFALASAVGLWLLVVPPDLTGSDAPEGPSGPVGAPVATEQPTDGEPTGTPP
                     AEATSTPGGGEQRYTVQAGDTLVDIASQFGTTPEDIMEANNLDSEIIQPGQELVIPE"
     gene            complement(1072..1464)
                     /gene="rplL"
                     /locus_tag="VNL92_00635"
     CDS             complement(1072..1464)
                     /gene="rplL"
                     /locus_tag="VNL92_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HWO93251.1"
                     /translation="MASKVDEAFDVIKGMTILELRDLNKRIEEEFGVTAAAPVAVAAA
                     APSAGGAAPAAEAEEEKTEFNVVLKEFGANKINVIKAVREVTSLGLKEAKDLVEAAPT
                     NVKEGITKAEAEEVRAKLAEAGATVEVQ"
     gene            complement(1499..2029)
                     /gene="rplJ"
                     /locus_tag="VNL92_00640"
     CDS             complement(1499..2029)
                     /gene="rplJ"
                     /locus_tag="VNL92_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HWO93252.1"
                     /translation="MPTAKKVQQVEELRDLVSRATIAISTDYRGLTVSQLQALRRRLR
                     DAGVEIHVVKNTLAHRAATEAGREGLSQIIEGPTALAIGFDDVVAPAKALTEFVRQSR
                     LTVPIRGAYTDGQVLSSADVADLASAPPREELLARIAGGLQSPLVNLMALLSATVREL
                     AGLIDARAGQLEGSEG"
     gene            complement(2232..2948)
                     /gene="rplA"
                     /locus_tag="VNL92_00645"
     CDS             complement(2232..2948)
                     /gene="rplA"
                     /locus_tag="VNL92_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWO93253.1"
                     /translation="MPKHGKKYRAALEKYEPERLYEPAEAIRIVKDAAYASFDETVEL
                     HMRTSLDPRHADQQIRATALMPHGLGKTVRVVVFAEGEGERLALEAGADEAGGDELIR
                     KVEGGYTDFDVALAQRELMGKVGRLGRILGPRGLMPNPRAGTVVDAADLPKAIRDARQ
                     GRVEFRLDRTALLHAPIGKVSFEETALLDNLATLIDAIQKAKPAGAKGQFVRTAALTS
                     TMGPGVKLDLRSTLAMQAPV"
     gene            complement(2964..3386)
                     /gene="rplK"
                     /locus_tag="VNL92_00650"
     CDS             complement(2964..3386)
                     /gene="rplK"
                     /locus_tag="VNL92_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWO93254.1"
                     /translation="MAKKVRAVVKLQIPAGKATPAPPVGTALGQHQINIMGFVKEYNE
                     RTSSRMGTIVPAEITIYEDRSFTFELKEPPASELLRQAAGIDKGSAAQRREKVGSVTR
                     QQLEAIAQQKMRDLNAYDLDAACRILEGTARSMGIEVQ"
     gene            complement(3421..4044)
                     /gene="nusG"
                     /locus_tag="VNL92_00655"
     CDS             complement(3421..4044)
                     /gene="nusG"
                     /locus_tag="VNL92_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWO93255.1"
                     /translation="MVSELEDEQVASAAAEDEVEQLDDVDLAEEPQDGREWYVIHTWS
                     GYENKVKKNLESRIESMDVRDKIFHVIVPTEDEIEIRDGQRRTVARKVFPGYVLVEMI
                     MDDESWYVVRNTPGVTGFVGMGNRPTPLGRDEVGNIIRQMRAEAPRVKVGFRVGDGVR
                     IIDGPFVDFVGQVDEINLQKGKVKVLVSFFGRETPVELDFLQVERLT"
     gene            complement(4083..4406)
                     /gene="secE"
                     /locus_tag="VNL92_00660"
     CDS             complement(4083..4406)
                     /gene="secE"
                     /locus_tag="VNL92_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWO93256.1"
                     /translation="MNRRAARRQAPAQQQSKQPRAQLRTGQRSQTAAARKAAAERRPR
                     GFRAILYPEWLRNIVAELRKVTWPTRQETQNLTVVVVTVAIAIGIVLGVFDFVFSWVI
                     ERTLL"
     gene            complement(4450..4617)
                     /gene="rpmG"
                     /locus_tag="VNL92_00665"
     CDS             complement(4450..4617)
                     /gene="rpmG"
                     /locus_tag="VNL92_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWO93257.1"
                     /translation="MAKKKEDRPFIHLACTECRSRTYTTTKNRRNDPDRLELRKFCPR
                     CRRHQLHRETR"
     gene            complement(4633..>4835)
                     /gene="tuf"
                     /locus_tag="VNL92_00670"
     CDS             complement(4633..>4835)
                     /gene="tuf"
                     /locus_tag="VNL92_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813659.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWO93258.1"
                     /translation="PQFYIRTTDVTGSIALPEGVEMVMPGDNVKMTIELIQPIAIEDG
                     LRFAIREGGRTVGAGVVTKVLQ"
BASE COUNT          743 a         1627 c         1520 g          945 t
ORIGIN      
        1 gctccggccc agcacgggcc tagtcgttct gccgaacggt cggcgcttgc gggtctgagg
       61 attctgagcg atgggccggc ggctcgtcat catcgtcagt cgccggcgaa gggcctgtct
      121 cgggagggga cggctccggc cggaccttgg cgatcgtttc gatggtggcc gtatgcggca
      181 tgtgcgtgat gaccacttcg tcgccctcct tgaggacgac atgcgtctcg ggccgcacct
      241 tgaacacggt ttcgtccacg tggatgtagt agctgcgcca gaagaagaag tcggagcgcg
      301 accaggaccg ccgcaccctt ccgcgagtct ccttgagctc cgcacgaagg tcgcgcagcg
      361 cttcgatgct ctggaagccc accagcagcg caataaggcc aacgatgacg atgaggacga
      421 ggctggcatc cccgcccgtc gcgcggttca cgatcatcgc gatgaggacg gcgagtgtca
      481 ggagcaacag cggcgtaaag atgatggcgc tgcgcacgac cgaccgccga gcctcgcccg
      541 tggtgaactc gtagcccggt aaccggtcta tcattctggg atgaccagct cctgccccgg
      601 ctgaatgatc tcggaatcga ggttgttcgc ctccatgata tcctccggag tcgtcccgaa
      661 ctgcgatgcg atgtcgacga gcgtatcgcc cgcctgcacc gtgtagcgct gttcgcctcc
      721 gccaggcgtg ctcgtggcct cggccggcgg cgtgccggtc ggctcgccat cggtgggctg
      781 ctcggtcgcc acgggcgcgc cgactggccc gctggggccc tcgggggcat ccgatcccgt
      841 aaggtcgggc gggacgacga ggagccacag gccgacggcg ctggcgagcg cgaacgcgag
      901 cagcgatcct cggtaccatt gctgcgaggg tccggcagcg gggttcgcgc atcgtgcgca
      961 tatctcgccg ccgtgctcgc tgcaatacgc agttccgcaa cgcggacagc tcgcggtggc
     1021 ggtggacgca caggcgtagc aggtcgtcat cagggctcgc aggagacctg gctactgaac
     1081 ctcgacggtg gcgccggcct cggcgagctt ggcccggacc tcctctgcct cggccttggt
     1141 gatgccctcc tttacgttcg tcggggccgc ttcaaccaag tccttcgcct ccttgaggcc
     1201 gaggctcgtc acttcacgca cggccttgat gacgttgatc ttgttggcgc cgaactcctt
     1261 gagcacgacg ttgaactcgg tcttctcctc ttccgcctca gcggcgggcg ctgcgccgcc
     1321 ggcagatggc gccgctgctg ccaccgcgac cggcgccgca gccgtcacgc cgaactcctc
     1381 ctcgatgcgc ttgttgaggt cgcgcaattc gaggatggtc atgcccttga taacgtcaaa
     1441 tgcctcgtcg accttgctgg ccatgttctc ctccttcttt cacgctctcg cgtgtcgatc
     1501 agccttccga gccttccagt tgtcccgcgc gtgcatcgat gagccccgcc aactcgcgca
     1561 cggtggcgct gaggagcgcc ataagattga cgagcggcga ttggaggcct cccgcaatcc
     1621 gtgccaacag ttcctcccgg ggcggcgccg acgcaaggtc cgcgacatcg gcgctgctca
     1681 ggacctgtcc atcggtgtag gcgccccgaa tcggtaccgt gaggcggctc tgccgcacga
     1741 attccgtgag cgccttcgcc ggcgcgacga catcgtcgaa gccgatggcg agcgccgtcg
     1801 gtccctcgat gatctggctg aggccctcgc ggcccgcctc agtggccgcc cggtgtgcga
     1861 gggtgttctt gacgacgtgg atctcgacgc cagcgtcgcg caaccggcgt cgcagcgcct
     1921 gaagctgtga gaccgtgaga ccacgatagt ccgtgctgat ggcgatggtg gcgcgcgaaa
     1981 cgagatcgcg caactcctcc acctgttgaa ccttcttcgc cgttggcatg ccgcctcctc
     2041 gtaacgcgcc tgggtaagac aactgccccg acacgaggtg acggggcagc acaaccggca
     2101 gcatccggaa gcatgggtgc cgcgtccctc acctcggcag gtatcgcttc ggcccttggc
     2161 gggcgcctgc ggtcttcggt gggcgcgtcg tattcgattg tggtcgtggt tcggctctcg
     2221 ccgctcctat cttacactgg cgcctgcatt gcaagcgtgg aacgcaaatc gagcttcacg
     2281 cccggcccca tcgtgctggt cagcgcggcc gtcctcacga actggccttt ggctccggca
     2341 ggcttcgcct tttggatcgc gtcgatcagg gtagcgagat tgtcgagcag cgcggtctcc
     2401 tcgaagctga ccttgccgat aggggcgtgc agcagcgccg tccggtcgag gcggaattcg
     2461 actcgaccct ggcgcgcatc gcggatcgcc ttcggcaaat cggcagcatc gacgacggtg
     2521 ccggcgcgcg gattgggcat gaggcctcgc ggaccgagaa tgcgtcccaa ccgcccgacc
     2581 ttccccatga gctcgcgttg cgcgagcgcg acgtcgaagt cggtgtaacc gccctccacc
     2641 ttgcggatga gctcgtcacc gccggcctcg tccgcgccgg cctccagcgc gaggcgctcg
     2701 ccttcgcctt ccgcaaagac gaccacgcgg accgtctttc cgagcccatg tggcatgagc
     2761 gccgtggccc ggatctgctg atcggcgtgg cgagggtcga ggctcgtgcg catatggagc
     2821 tcgacggtct catcgaagct ggcgtaggca gcgtccttga cgatgcggat ggcctcggca
     2881 ggctcgtaga gccgctcagg ctcgtacttc tcgagggccg cccgatactt ctttccgtgc
     2941 ttaggcatcc cgcactcctc cgctcattga acctcgatgc ccatggacct tgccgtaccc
     3001 tcgagaatcc ggcacgccgc atcgaggtcg tacgcgttga ggtcgcgcat cttctgctgg
     3061 gcaatggcct cgagctgctg gcgggtcaca cttcccacct tctcacggcg ttgtgccgcc
     3121 gaacccttgt cgatgccagc cgcttgccgg agcagctccg acgccggtgg ctccttgagc
     3181 tcgaacgtga acgaccggtc ctcgtagatc gtgatctcgg caggcacgat ggtgcccatg
     3241 cgcgaagacg tgcgttcgtt gtattccttg acgaagccca tgatgttgat ctggtgctga
     3301 ccaagcgccg tcccgacggg cggagcaggg gtcgccttac cggcgggaat ctgtagcttg
     3361 acgacagcgc gaaccttttt tgccacgcgg gctctccctc gggtggatgg cgctcggacc
     3421 ttacgtcagg cgctcgacct gaagaaagtc gagctcgacg ggtgtttcgc gtccgaagaa
     3481 ggacacgagg accttcacct tccccttctg tagattgatc tcgtcgactt ggccgacaaa
     3541 gtccacgaac gggccatcga tgatgcgcac gccatcgccc acgcgaaagc cgaccttgac
     3601 gcggggcgct tccgcccgca tctgccggat gatgttgccc acctcgtccc gtccgagcgg
     3661 cgtgggccgg ttccccatcc cgacgaatcc ggtcacgccg ggcgtattac gcacgacgta
     3721 ccacgactcg tcgtccatga tcatctcgac gaggacgtag ccagggaaga ccttgcgggc
     3781 aacagtgcgc cgctggccat cgcggatctc gatttcgtcc tcggtgggca cgatgacgtg
     3841 gaagatcttg tcccgcacgt ccatcgactc gatgcggctt tcgaggttct tcttcacctt
     3901 gttctcgtag cccgaccacg tgtggatcac gtaccattcg cggccgtcct gaggctcctc
     3961 ggcgaggtcg acatcgtcaa gctgctcgac ctcgtcctca gctgcagcag acgcgacttg
     4021 ctcgtcctcc agctcgctta ccatgcggtt cttcttcgtt gccataggtc acttcaatcc
     4081 atttacagaa gggtccgctc gatgacccag gaaaagacga agtcgaagac gccgagcacg
     4141 atgccgatgg cgatggccac ggtaaccacg acgaccgtca ggttctgcgt ctcctggcga
     4201 gtcggccatg tgaccttgcg cagctcggcc acaatgttgc gcaaccactc ggggtagagg
     4261 atggcgcgga aaccacgtgg gcgtcgctcg gccgccgcct tgcgggccgc cgccgtctgc
     4321 gatcgttgac ccgtgcgcag ttgcgctcgc ggctgcttcg actgctgttg tgctggtgcc
     4381 tgacgccggg cggcccttct attcatgcgt gtttcctacg tcccgttcgt gagccaggca
     4441 tcgcgcgcac tatcgcgtct cgcgatgcag ctggtgccgg cggcagcgcg ggcagaactt
     4501 gcgtagctcc aaccggtccg ggtcgttccg ccgattcttg gtagtcgtat aggtcctcga
     4561 cctgcattcg gtgcaggcga ggtggatgaa cggacggtct tccttcttct tcgccacttg
     4621 gctttcccag ctttactgca gaaccttcgt caccacgccg gcgcccacgg tccggccgcc
     4681 ctcgcgaatg gcgaaccgca agccgtcctc gatcgctatc ggctggatca gctcgatggt
     4741 catcttcacg ttgtccccgg gcatcaccat ctccacgccc tccggcaacg caatcgaccc
     4801 cgtcacgtcc gtcgtccgaa tgtagaactg gggcc
//
LOCUS       DATMBB010000033         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_273955, whole genome shotgun sequence.
ACCESSION   DATMBB010000033 DATMBB010000000
VERSION     DATMBB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273955"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VNL92_00675"
     CDS             <1..134
                     /locus_tag="VNL92_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HWO93259.1"
                     /translation="LEGGGWLLIRFSGTEPLMRIYTEVRDEALVPKLLEAGREIAGV"
     gene            180..1268
                     /locus_tag="VNL92_00680"
     CDS             180..1268
                     /locus_tag="VNL92_00680"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HWO93260.1"
                     /translation="MGIADAALLDDAERMRRQDPGDMLGRIRELPEQVVAAWRDSRGA
                     PIGDEYREVDHIVVFAMGGSAIGADLLRTLMLSESPVPMIVVRGYDAPAFVGPRTLAV
                     ASSYSGGTEETLSAFGQALERGARAFVLTGGGELLRMAGERGLPFHIITYEAMPRAAL
                     AHSLMPLAALVSKLGFLSLDDGDVEAAARAMQARRDAVDAPAPLESNEAKRLALRLAG
                     RVPIIIGAEHLSEVARRWRGQIAENGKAWSFTDELPELNHNTVVGLPNPQVVLESATA
                     IFLSSPALHQRTLLRYDLTAQMLKGAGIESVRVEADGATRLGDLLSLVMLGDYVSYYL
                     AMLYGADPTEVRVIERLKRELSEATPLP"
     gene            complement(1252..2472)
                     /gene="metK"
                     /locus_tag="VNL92_00685"
     CDS             complement(1252..2472)
                     /gene="metK"
                     /locus_tag="VNL92_00685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWO93261.1"
                     /translation="MTTTFERSPSLFLTSESVTEGHPDKMCDQISDAVLDAIIAQDPT
                     ARVACETAVTTGLVLVFGEITTSCYVDIPKVIRNTIREIGYTSPEYGFDYETCGVMTS
                     IDEQSPDIAQGVDRSLELRLGASEDEELERGAGDQGMMIGFACDETPELMPATIALAH
                     RLCRRLAEVRKDGTLPYLRPDGKSQVTIEYAYGKPRRVEAVVLSSQHDEYVEHHVLEQ
                     ELIEQVIHHVIPANLLDEHTKYYVNPTGRFVVGGPVGDAGLTGRKIIVDTYGGIGRHG
                     GGAFSGKDPSKVDRSGAYAARHVAKNVVAAGLAGRCEVQVSYAIGVARPTSLAIETFG
                     TGVIPDEEILRLVRKHFDLRPAAIVRELNLLRPIYRNTAAYGHFGRLDIDAPWEQTDK
                     AAGLRADAGIKATA"
     gene            complement(2504..>2762)
                     /locus_tag="VNL92_00690"
     CDS             complement(2504..>2762)
                     /locus_tag="VNL92_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWO93262.1"
                     /translation="GEGRLINLAAAEGHPASVMDMSFANQALCAEYLAENASSLERGV
                     HIVPRDLDAEVARLKLASMGIQIDTLTDEQATYLAAWESGT"
BASE COUNT          431 a          867 c          961 g          503 t
ORIGIN      
        1 tgctcgaggg cggcgggtgg ttgctcatcc gattctccgg taccgagcca ctcatgcgca
       61 tctacacgga agtgcgcgac gaggcgctcg tcccgaaact gctcgaggcc gggcgggaga
      121 tcgcgggcgt ctgacggcgc ggcctggcgc gctgcccgta gaccgttgag gaggacccca
      181 tgggaatcgc cgatgccgcg ctgcttgatg acgcggagcg gatgcgacgg caggaccccg
      241 gcgacatgct cggtcgcatt cgcgagctgc ctgagcaggt ggtcgcggcc tggcgcgatt
      301 cacgtggcgc gccgattggc gatgagtatc gcgaggtcga ccatatcgtc gtcttcgcca
      361 tgggcggctc ggccataggc gccgacctgc tgcggacgct catgctctcg gaaagccccg
      421 tcccgatgat cgtcgttcgc ggttatgatg cgccggcctt cgtgggcccg cgcaccctcg
      481 ccgtggcgtc gagctatagc ggcggtacgg aggagacgct ctcggcattc ggccaggctc
      541 tcgagcgggg tgcacgggcg ttcgtcctca ccggaggcgg cgagttgctc cggatggcgg
      601 gggagcgggg attgcctttc catatcatca cgtacgaggc catgcctcgc gcggccctgg
      661 cgcattcgct gatgccgctc gcggccctcg tcagcaagtt ggggttcctc tcgctcgatg
      721 atggcgacgt ggaggcggcc gcacgtgcga tgcaggctcg ccgcgacgcg gtcgacgcgc
      781 cggcgccgct ggaatcgaac gaggccaaac ggctggcgct gcggctcgcg gggcgcgtac
      841 cgattatcat cggcgccgag catctcagcg aggtggcgcg gcgctggcgc ggacagatcg
      901 ccgagaacgg gaaggcttgg tccttcaccg atgagctgcc ggagctgaac cacaacacgg
      961 tggtcggcct gcccaaccct caggtcgtgc tggaaagcgc gacggcgatc tttctgtcgt
     1021 cgccggccct gcaccagcgg acgctgctcc gttatgatct gacggctcag atgctgaaag
     1081 gggcgggcat cgaaagcgtc agggtcgaag ccgatggtgc gacgaggctc ggcgacctgc
     1141 tgagcctcgt gatgctcggg gattacgtca gctactacct cgcgatgctc tacggggccg
     1201 accccaccga ggtacgggtt atcgagcggc tgaagcggga gctttcggaa gctacgccgt
     1261 tgccttgatg cccgcgtcgg cccgcaagcc ggcggcctta tccgtttgct cccaaggagc
     1321 atcgatatcg aggcgaccaa agtggccata cgccgccgta ttgcggtaga tcggcctaag
     1381 cagatttagc tcccgaacga tggcggcagg gcgcaggtcg aagtgcttgc ggacgagacg
     1441 caagatctcc tcgtccggta tgacaccggt gccgaaggtt tcgatggcga gcgaggtggg
     1501 gcgtgccacg ccgatggcat acgacacttg cacctcgcac cggccggcga gacccgccgc
     1561 gaccacgttc ttggcgacgt ggcgagcggc atacgccccg gatcggtcga ccttcgaggg
     1621 gtctttgccg gagaacgcgc ctccaccgtg ccggccgatg ccgccgtacg tatcgacgat
     1681 gatcttgcgt cctgtgagcc cggcatcgcc aaccgggccg cccacaacga agcggccggt
     1741 gggattcacg tagtacttcg tgtgctcatc gaggagattg gccgggatga cgtggtggat
     1801 gacctgttcg atgagctcct gctccaggac gtgatgctcg acgtactcgt cgtgctggga
     1861 cgacaggacg acggcctcga cgcgacgcgg cttgccgtag gcgtactcga tcgtcacctg
     1921 cgatttgccg tccggccgaa ggtacggtag cgtgccatcc ttgcgcacct cggctaagcg
     1981 ccggcacagg cggtgggcga gcgcgatggt ggcgggcatg agctccggcg tttcgtcgca
     2041 ggcaaagccg atcatcatgc cctggtcgcc ggcgccgcgc tccaactcct cgtcttcgct
     2101 tgcccccagc ctgagctcca gcgagcggtc gacgccttgg gcgatgtcag gagattgctc
     2161 gtcgatggac gtcatcacgc cgcaagtttc gtagtcgaag ccgtactctg ggctcgtgta
     2221 gccgatctcc cggattgtgt tgcggatgac cttggggatg tcgacgtagc agctcgtcgt
     2281 gatctcgccg aacacaagga ccaggccggt cgtgaccgcg gtctcgcagg cgacacgggc
     2341 cgtagggtcc tgcgcaatga tcgcgtcgag gacggcatcc gagatctggt cgcacatctt
     2401 gtccgggtgg ccctccgtga ccgactcgga cgtgaggaac agcgaaggtg agcgctcgaa
     2461 ggttgtcgtc attctcttgg agtcctctct cgcccgttgt gagtcaggtg ccgctttccc
     2521 aagcggccag ataggtcgcc tgctcgtcgg tcagggtgtc gatttggatg cccatcgagg
     2581 cgagcttgag ccgtgcgacc tctgcatcga gatcgcgcgg gacgatgtga acaccccgct
     2641 cgagcgagct cgcgttttcg gcgaggtact cggcgcacag cgcctggttc gcgaagctca
     2701 tgtccatgac gctggcgggg tgcccctcgg cggctgcgag gttgatcaac cggccctcgc
     2761 cg
//
LOCUS       DATMBB010000034         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_469797, whole genome shotgun sequence.
ACCESSION   DATMBB010000034 DATMBB010000000
VERSION     DATMBB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469797"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..701)
                     /locus_tag="VNL92_00695"
     CDS             complement(174..701)
                     /locus_tag="VNL92_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWO93263.1"
                     /translation="MVSTPVLVLNQMYEPLNVCNVRRAFVLVDRGKAEVLERGDEWIR
                     SVSAAFPTPSVIRLMTQIRRPRPTVRLSRREVFQRDKYTCQYCGQVARDLTLDHVIPR
                     HRGGRHTWENLVTACKRCNHRKAGRTPSEARMRLSTEPTAPRYGLYAAFWPYLEREEG
                     WRKFVPGWDPDDGFA"
     gene            1002..1736
                     /locus_tag="VNL92_00700"
     CDS             1002..1736
                     /locus_tag="VNL92_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWO93264.1"
                     /translation="MQTIILAAGDGGRLHPATASRPKPLLPLAGRPIIDYVLDAFVAC
                     GLREFIVVVGYEGAQIQRHLLPRQDVAINFAWNADPLAGNARSLSNAAPLLRPAPFLL
                     AMADHLQSPTSIQAAIHAPPGSSWVMVDSLAPLDVQMEGTRVQLGPDGSVRAIGKNLP
                     AWDAIDIGLFRLEPTALEHVRLLPPAAELNDAWQTLVAAGGLRAIDVAGAWWADIDTA
                     VDLARAETALMEARSNAARRPGLALP"
     gene            1702..2295
                     /locus_tag="VNL92_00705"
     CDS             1702..2295
                     /locus_tag="VNL92_00705"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWO93265.1"
                     /translation="MPLEGPVSRYLNRRLSRPLARAAERAGLTPNQVTLLSTASVLAV
                     PALFALDRPRLAGLAIHAASVVDGVDGDLARATGQTSTFGAILDAVSDRYVDAAIIAS
                     MTWWSAKRERRPFTVPAGLAALVGSLMVSYSRARTEAETRATLDASLQGYATRDVRLL
                     LAALGSATRHVDTALLTLALMTNASVLRRLVRLRSQL"
BASE COUNT          404 a          839 c          750 g          422 t
ORIGIN      
        1 tgatgccgtc gaggaagcgg gcgcagacgt cgccctcacg cttgcgatcg tcgaccggca
       61 agagggtggg gcggaggcgc tacgcgcgcg aggctaccag tttcgggcgc tcttccgtgc
      121 cgacagcggc ggaaggctca ccatcgccca gtagccgccc acgacagtgg gcctcaggcg
      181 aatccgtcgt ccgggtccca accaggaacg aacttgcgcc agccttcctc gcgttccagg
      241 tatggccaaa acgccgcgta gagtccatac cgcggagccg tgggctccgt cgacaatcgc
      301 atgcgtgctt cgctcggcgt gcgccccgcc ttacgatggt tgcagcgctt gcatgcagtg
      361 acgagattct cccaggtgtg ccgacctccc cggtggcgcg ggataacgtg gtccagcgtc
      421 aggtcccggg cgacttgccc gcagtactgg caggtgtact tgtcacgctg gaacacttct
      481 cggcgggaaa gacgaaccgt cggccggggc cggcggatct gggtcatcag cctgatgacg
      541 gacggagtgg gaaaggcggc ggacacgctc cgaatccact cgtcgccgcg ttccaacacc
      601 tcagccttgc cacggtcgac gagcacgaag gcgcggcgca cgttgcacac attcagcggc
      661 tcgtacatct ggttcaggac gaggacagga gtacttacca tggccggccc atcgctccga
      721 gactcgcctc tgactggatg atagcagtgc caacgaagcc tgcgcaatcg tttgcacccg
      781 tccggcgcat actagacgag acggcgagga ccgcgttatg tcgagccgcg tggagagtta
      841 caatgctgtt tcatctggca ggattccgtt gacagcccgc tcgtcccacc cctatcctcc
      901 ctccataccg gctggtctga cggccctgac cgttcttatg ccgaccggct cgcgtccttc
      961 cctcaggccg cgacctgagc caagagacat acggagcctt gatgcaaacg ataatcctcg
     1021 ccgccggcga tggcggtcga ctacatcccg cgacagcttc acgtccgaag ccactcctgc
     1081 cactcgcggg ccggcccatc atcgattacg ttctcgatgc tttcgtcgct tgtggacttc
     1141 gagagtttat cgtcgtggtt ggatacgagg gggcccagat tcagcgccac ctgctgccac
     1201 gacaagacgt cgctatcaac ttcgcctgga atgccgaccc gctcgccggg aacgcgcggt
     1261 ccttgagcaa cgcagcgccg ctccttcgtc ccgcgccctt cttgctggcc atggccgacc
     1321 atctccagag tcccacgagc atccaagcgg cgatacatgc cccaccgggc agcagctggg
     1381 tgatggtcga tagtctcgca ccactcgacg tccagatgga gggcacgcgc gtgcaactgg
     1441 ggcccgacgg gtcggttcgc gccatcggga aaaacctgcc ggcctgggac gccatcgata
     1501 tcggcctgtt ccggctcgag cccacggcgc tcgagcacgt ccgccttctg ccgcccgcgg
     1561 cggagctcaa cgatgcttgg cagaccctcg tggcggcggg cggcctgcgg gcgatcgatg
     1621 tcgctggagc gtggtgggcc gacatcgaca cagccgtcga cctcgcccgc gccgagaccg
     1681 cactcatgga agcgaggtcg aatgccgctc gaaggcccgg tctcgcgcta ccttaaccga
     1741 aggctctcgc ggccactggc acgagccgcc gagcgcgctg gcctcacgcc caaccaggtg
     1801 acccttctca gcacggccag cgttctcgcc gtcccggcac tcttcgcgct cgaccggccg
     1861 cggctcgccg gactcgccat ccacgccgcg tcggtcgtcg acggcgtcga cggcgacctc
     1921 gcccgcgcca ccggccagac ctcaacattc ggcgccatcc tcgatgccgt gtctgaccga
     1981 tacgtcgatg ctgccatcat cgcgagcatg acgtggtggt cggccaagcg cgaacggcgc
     2041 cccttcaccg tgccggcggg cctggctgcg ctcgtcggca gcctcatggt aagctattcg
     2101 agagcacgga cggaggcaga gacccgtgcg acgctcgacg catcgctcca gggttatgcc
     2161 acccgggatg tccgcctcct gctcgccgcc ctagggagcg ccacgcgaca cgtcgatacc
     2221 gcgctcctga ccttggcact catgacgaac gcgagcgtgc ttcggcgcct cgtgcggctc
     2281 cgttctcagt tgtaggacgg cgatgatggg ttccccagga cgcgggattg accgagagga
     2341 cgaccttcga tggaccttga gaccgtgcgc gattgggtca tcatcgtgta cggcattttt
     2401 ggtatcatca ccttc
//
LOCUS       DATMBB010000035         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_391979, whole genome shotgun sequence.
ACCESSION   DATMBB010000035 DATMBB010000000
VERSION     DATMBB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391979"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VNL92_00710"
     CDS             complement(<1..560)
                     /locus_tag="VNL92_00710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWO93266.1"
                     /translation="MARDEAAFPDGELLAGRYRIEEVVGRGGMAVVYRAIDTLLGRTV
                     ALKELAEPLSSQRSGQQLVDEARAAAALSHPHIVTVYDTFDADSRRYIVMEYVPGGSL
                     RELLDRAGTLDAETAVDIAYEVADALDYAHRHGIIHSDVKPHNILLDAGGHAKLVDFG
                     IARAAAQTWALATTVMGSAAYLAPEQ"
     gene            complement(652..987)
                     /locus_tag="VNL92_00715"
     CDS             complement(652..987)
                     /locus_tag="VNL92_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93267.1"
                     /translation="MSQRNGEGPPVVTVSEEAARRIRESLGQHPESIVLRIAFHIRDE
                     QPVHAIMPEPGPKQNDLVFVQHGLKFVVDLGTLPLIRGSHIYWDPELQGIEVDNPNIE
                     VAEEDEDLR"
     gene            1084..1752
                     /locus_tag="VNL92_00720"
     CDS             1084..1752
                     /locus_tag="VNL92_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HWO93268.1"
                     /translation="MNRYYFDLHTHSTDASDDAGATVEGYLKWIVGRRKKGYRVDGLV
                     LTEHRSFDFEVDYTALAEEYGVVVLKGAELETEIGHVLVYGITAAFAREFDLSRVDLP
                     SKDVFAAARELGGFAVGAHAGRPRIGIWDHAERGASLDHVTSIEQLNGGSSHDENARA
                     LVLAERHGLRTIGGSDAHYVSAVGRCLTAFERPITCIEDLVEQLRGGDYAPVRIEDTF
                     LGIE"
     gene            1762..>2034
                     /locus_tag="VNL92_00725"
     CDS             1762..>2034
                     /locus_tag="VNL92_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO93269.1"
                     /translation="MAEAAELEFDRSVLGKEVEVGRLEVRREHILAFCQAVGETNPLY
                     TDEREAATGPYGGIIAPPVFYACIPVQQGLDPKVTFGNTILNAGQHC"
BASE COUNT          358 a          649 c          664 g          363 t
ORIGIN      
        1 atctgctcgg gagcgaggta tgcagccgac cccatcaccg tcgtcgcgag cgcccacgtc
       61 tgcgccgccg ctctcgcgat tccaaaatcg acgagcttcg cgtgccctcc agcatcgagg
      121 aggatgttgt gcggctttac atcggagtga attatgccgt ggcgatgcgc atagtcgagc
      181 gcatccgcta cttcgtacgc gatatctacg gccgtttccg cgtcgagcgt tcctgcccta
      241 tcgagcagct cgcgtaggct gccgcccggc acgtactcca tcacgatgta gcgccggctg
      301 tccgcatcga acgtgtcgta cacggttacg atgtgagggt gcgagagggc ggctgccgcc
      361 cgagcctcgt cgacgagctg ttggccgcta cgctgcgagc tcagtggctc ggccagctcc
      421 ttgagcgcta cggtccgacc gaggagcgtg tcgattgcgc gatacaccac ggccatgccg
      481 ccgcgaccga cgacctcttc gattcggtac cggccggcga gcaactcgcc gtccggaaac
      541 gctgcttcgt cccgcgccat ccagacccct ccggggcgac atcgcccccg cccatcttac
      601 gagcgagggc gatgagcgtc gaacgggacg cggtggaggg gaggacgtgg tctaccggag
      661 gtcctcgtct tcctcggcga cttcgatatt cggattgtcg acctcgatgc cctgcagctc
      721 cggatcccag tagatgtggg aaccgcggat gagcggcagt gtcccgagat cgaccacgaa
      781 cttgaggccg tgctggacaa agacgaggtc gttctgcttg ggccccggct cgggcatgat
      841 ggcgtgcacg ggttgctcat cgcgaatgtg gaatgcgatc cgcaacacaa tgctctcagg
      901 atgctgcccg agcgattcgc gtatacggcg agccgcctcc tcggagaccg tgacgaccgg
      961 cgggccctct ccgttcctct gcgacattgg cacctccctg cggcatcatc agcctaccat
     1021 tgaatcggac aaatcgggca agagattgcg gggcggttgg cgactcctgt accatgcacc
     1081 ccagtgaacc gttactattt cgatcttcac acacattcca ccgatgcctc cgacgatgca
     1141 ggcgccacag tcgaggggta cctgaaatgg atcgtcgggc ggcgaaagaa gggctatcgg
     1201 gtcgacgggc ttgtactcac cgagcaccgc agcttcgact tcgaggtcga ttacacggcc
     1261 ctcgcagagg agtacggcgt cgtcgtcctg aagggggccg agctcgagac cgagatcggt
     1321 cacgttctgg tctacggcat caccgcggcg ttcgcccgcg agttcgacct gagccgcgtc
     1381 gatctgccat cgaaggacgt ctttgcggcc gcccgcgagc tgggcgggtt cgcggtggga
     1441 gcgcacgcgg ggcgaccgcg cattggaatc tgggaccatg cggagcgtgg cgcctcgctg
     1501 gaccatgtga cctcaatcga gcagttgaac ggcggcagca gccacgacga gaacgcacgc
     1561 gcgctcgtcc tcgcggagcg gcatgggctc cgcactatcg gtgggagcga tgcccattac
     1621 gtgagcgcag ttggccggtg ccttaccgcc ttcgagcggc cgatcacgtg catcgaggac
     1681 ctcgtcgagc aactgcgtgg tggcgactat gcgccggtcc gcatcgagga cacgttcctc
     1741 ggcatcgagt aggagtggag catggctgag gcagcggagc tggagttcga ccgcagcgtc
     1801 ctcgggaaag aggtggaggt cgggcggctc gaagtgaggc gagagcacat tctcgcgttc
     1861 tgccaagccg tgggcgagac gaacccgctg tacaccgacg agcgcgaggc ggcgaccggg
     1921 ccatacggcg gcatcatcgc accccccgtc ttctatgcct gcatcccagt tcagcagggg
     1981 ttggacccca aggtcacctt cggaaacacg attctcaatg cgggccagca ctgc
//
LOCUS       DATMBB010000036         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_156118, whole genome shotgun sequence.
ACCESSION   DATMBB010000036 DATMBB010000000
VERSION     DATMBB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156118"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VNL92_00730"
     CDS             <1..446
                     /locus_tag="VNL92_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWO93270.1"
                     /translation="SHIPAIVQDTRFDGILSGHQDGGMRLEMLPNAASVEAGDTIVTS
                     GLNGELPKGLPLGRVIEVGGSDQDPFPSVFVEPLTALDRLEHLVVLTSFRPGGIPDAE
                     SVEEAAPEQGALAAADAPQTEEATAQAGQGAEQRPDESRLPGSGQ"
     gene            443..955
                     /gene="mreD"
                     /locus_tag="VNL92_00735"
     CDS             443..955
                     /gene="mreD"
                     /locus_tag="VNL92_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWO93271.1"
                     /translation="MSIIIGGMWVTFVVLWESAGLPLPALFGAPADVLLLIVFAWAYV
                     RSPEEAIGVAIIGGFAADLLSDHPLGITVLALLPATVIGSVRGARLLDTDWVSTMLLG
                     AAATLVYDVILLGLLRLSGDVSGLADALVHEAPAAALLNALLAPLVYLVVIAGSRDVR
                     AARRQIRTAG"
     gene            964..>2988
                     /gene="mrdA"
                     /locus_tag="VNL92_00740"
     CDS             964..>2988
                     /gene="mrdA"
                     /locus_tag="VNL92_00740"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWO93272.1"
                     /translation="MLSDPRDPKRWRRSRTESDETSEAEHRYYLVGIRIAILVLFAIL
                     TAQLFRMQIIQGSEYEERARNNRIATEVVHPLRGLIYARDGTPVVENEATFSAIVIPA
                     EIDEDEERQLAEQLERLLNVPAATVRQRIDEGRESIDPYDPVVIQEDIDRDVALTLRQ
                     MNSDVPGVDVRVDSKRHYLFGDLLTHVVGYVGPVNEQEYEELQDSGYTMNDILGKDGV
                     EAQYEGLLRGDVGYVQREVDAAGNEVRRLDEEAPEPGKGLVLSIDLELQKRVQELAYQ
                     HRGRSKNVVAIVMDVKTGEILAMVSVPTFDANILMDDEAQDEVAALFEDPGEPLLNHA
                     IGGVYPPGSTFKQITGLAALQEGVATPSTTIESKGYLEVLNDQGEFVHYAPDWAPLGV
                     LDFYRGVAMSSDVYFYYLAGGYAPEGFRGLGADRLAQYARAFGIGRPTGIDLPGEAPG
                     IVPDPEWKERTIGEPWVLGDSYNYGIGQGFVTTTPLQMLNATAAIANGGELLRPHVVR
                     QVVDDKGRVVVPSNRQVIGHLPISPENLAVMRQAMVLAVTEGTASPAAVSGVTVAGKT
                     GTAEFGVQLADGSYDTTHGWFTGFAPADDPQIAVVTFVEHGGGNQEAAPLAQEILDFY
                     FHRGTESSDDLSADDAPAADSPAATTDADAAPAGEDVAPVEEPDVPVEEPP"
BASE COUNT          513 a          966 c          998 g          511 t
ORIGIN      
        1 cgagccacat tccagcaatc gtgcaggata cgcgtttcga tggcattctg agcgggcatc
       61 aggacggagg catgcggctc gagatgctgc ccaacgcggc ctcggtggag gccggggaca
      121 cgatcgtcac gtccgggctg aacggcgagc tgccgaaagg cttgccgctc ggtcgcgtca
      181 tcgaagtcgg cggcagcgat caggacccct tcccctccgt gttcgtcgag cctctgacgg
      241 cgctcgatcg cctggagcat ctcgtcgttc tgacgagctt ccggcccggc ggcataccgg
      301 atgccgaaag cgttgaggag gccgcgccgg agcaaggagc gctcgcggcg gctgatgctc
      361 cgcagaccga agaggcgacg gcgcaagccg ggcaaggagc cgagcagcgg cccgacgaat
      421 ctcgattgcc aggcagcggg caatgagcat catcatcgga ggaatgtggg tcaccttcgt
      481 cgtcctgtgg gaatcggccg gtctcccgct gcctgcgctg ttcggcgcgc cggcggacgt
      541 gctgctgctc atcgtgttcg cctgggccta tgtccgtagc ccggaggagg cgataggcgt
      601 cgccatcatc ggtggattcg cggccgacct gctcagcgac catcctctcg gcatcacggt
      661 gctcgccctg ctgcctgcca cggtcattgg cagcgttcgc ggtgctcggt tgctcgatac
      721 ggattgggtc tccaccatgc tcctcggcgc ggcggcgacg ctggtctacg atgtcatcct
      781 ccttgggctc ctccggcttt ccggcgatgt ctcgggcctg gcggatgccc tggtgcacga
      841 ggcgcccgcg gccgccctgc tcaatgccct cctcgcgccg ctcgtgtacc tcgttgtcat
      901 cgcgggcagc cgtgatgttc gagccgcgag gcgccagatc cgcacggcgg gttagtccac
      961 gtcatgctat cggacccacg cgaccccaag cgttggcgcc gctctcggac tgaaagtgac
     1021 gagacgagcg aagccgagca tcgctactat ctcgtgggca tccgcatcgc tattctcgta
     1081 ctcttcgcca tcctcaccgc gcagctgttt cggatgcaga tcatccaggg ttccgagtac
     1141 gaggagcgcg cacggaacaa ccgcatcgcg accgaggtcg tgcatccgct gcggggcctc
     1201 atctatgcgc gcgacggtac gccggtcgtc gagaacgagg caacgtttag cgcaatcgtc
     1261 atacccgccg agatcgacga ggacgaggag cggcagcttg ccgagcagct cgagcgcctt
     1321 ctgaacgtgc ccgcggccac cgtccgccag cgcatcgacg aggggcgcga gagcatcgat
     1381 ccctacgatc ccgtcgtcat ccaggaggac atcgaccgcg acgtcgcgct caccctgcgg
     1441 cagatgaact ctgatgtgcc tggggtcgac gtcagggtcg actcgaaacg gcactatctc
     1501 ttcggcgact tgctcaccca cgtcgtcggt tacgtggggc cggtgaacga gcaagagtat
     1561 gaggagcttc aggatagtgg ctacacgatg aacgatatcc tgggaaagga tggggtcgaa
     1621 gcgcagtacg aggggttgct gcgcggcgat gtcggctacg tccagcgcga ggtcgatgcg
     1681 gccggcaacg aggttcgccg gctcgatgaa gaagcgccgg aaccggggaa gggcctcgtg
     1741 ctctccatcg acctggagct gcagaagcgc gtgcaggagc tcgcctacca gcaccgggga
     1801 cgctccaaga acgtcgtcgc catcgtcatg gatgtaaaga cgggcgagat cctcgcgatg
     1861 gtttcggtcc ccaccttcga tgccaatatc ctcatggacg acgaagctca agacgaagtg
     1921 gccgccctgt ttgaggaccc gggggagccg ctcctgaatc acgcgatagg tggtgtgtat
     1981 ccaccgggct cgacgttcaa gcagatcacg ggcctcgcgg cactccagga gggtgtggcc
     2041 acacccagca cgactatcga gagcaaggga taccttgagg tgctcaacga ccagggcgag
     2101 ttcgtgcact acgcgccgga ctgggcgccc ctcggtgtgc tcgatttcta ccgcggtgtc
     2161 gccatgtcgt cggacgtgta cttctactac ctcgcggggg gctacgcacc cgaggggttc
     2221 cggggccttg gggccgaccg cctcgcacag tacgcccgcg ccttcggtat cggacggcca
     2281 acgggcatcg acctgccagg agaggcgccg gggatcgtgc cggaccccga atggaaggag
     2341 cgcacgatcg gcgagccgtg ggtcctcggc gactcctaca actatggaat tggccaaggt
     2401 ttcgtgacga caacgcccct tcagatgctc aatgctacgg cggccatagc aaacggcggc
     2461 gagttgctcc gcccgcacgt tgttcgccaa gtcgtcgacg acaagggccg agttgtggtg
     2521 ccatcgaatc gccaggtgat tggccatctc cccatcagcc cggagaacct cgctgtcatg
     2581 aggcaagcga tggttctcgc ggtgaccgag ggtacggcca gcccggcagc cgtgagcggc
     2641 gtcacggtcg ccggcaagac cggtacggcg gagttcggtg tccagctcgc cgacggctcc
     2701 tacgatacga cgcacggctg gttcaccggc ttcgctcctg ccgacgaccc gcagattgct
     2761 gtcgtcacgt tcgtggagca cggcggcggc aatcaggagg cggcgccgct cgcccaggag
     2821 atcctcgact tctacttcca ccggggcacc gagagctcgg acgatctgag tgccgacgat
     2881 gcgccagccg cggattcgcc agctgcgacc accgatgcgg atgcggcccc tgctggcgag
     2941 gacgtggccc cggtggagga gcccgacgtc ccagtcgagg agccgccg
//
LOCUS       DATMBB010000037         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_190936, whole genome shotgun sequence.
ACCESSION   DATMBB010000037 DATMBB010000000
VERSION     DATMBB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190936"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..393)
                     /locus_tag="VNL92_00745"
     CDS             complement(46..393)
                     /locus_tag="VNL92_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93273.1"
                     /translation="MFANGKPVLVVRARVDDAVMAEFRRWHFTIHLPHMLAIPGVVGG
                     QIVKRNHERTPPINWQTIFEFSANSELQSALQSPQAQRAREDWAVWADYVSDLSIEVY
                     AELSALPAFHHWN"
     gene            complement(406..972)
                     /locus_tag="VNL92_00750"
     CDS             complement(406..972)
                     /locus_tag="VNL92_00750"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HWO93274.1"
                     /translation="MGGGVDVFRPRNIIVALLVIAVVGVLAYVIVDQINEPTAANEDS
                     SADIPGQFFEDLGRSHLQANQPYDEYNSNPPTSGPHDPVPAPWGVSADPIPKEKILHN
                     LEHGGVAVLYNCTDDCPDIVSELSAYVQGKVSDGDALLMAPYPGMEHRIALISWTRLD
                     AFDELDMERVERFVDVNLCRFDPENVCD"
     gene            1319..2596
                     /locus_tag="VNL92_00755"
     CDS             1319..2596
                     /locus_tag="VNL92_00755"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWO93275.1"
                     /translation="MQAISSELETKARAVKAAAPALARASTDLKNAALLAIARNIEER
                     VADVLTANAADCADSRAAGLSDAMLDRLTLTEGRLRSLAEDVRKVAALPDPVGEMFDV
                     RMLPNGLQVGRRRVPLGVIAVIYESRPNVTVDVASLALKSGNAALLRGGREAIRSNIA
                     LAAVVREAIAEVGLPEQTVELVESTDRAIVGELLGMRETIDIVVPRGGADFIRYVMDN
                     ATMPVIAHGDAVSHTYVDAAADVMKAVEIVVNAKCRRWGICNALDTLLVHEAIATPFL
                     QALAPRWAEYGIEARCDERAGAVLAHTPLAGRVRPVLPEDYGFEFLAPVAAVRVVDSL
                     EEAMAHIELHSSRHSEAIVTENYTAAQRFLEEVDSACVYVNASTQFTDGAQFGLGAEI
                     GISTQKMHARGPMGLRELTSYKWIILGSGQIRP"
     gene            2675..3007
                     /locus_tag="VNL92_00760"
     CDS             2675..3007
                     /locus_tag="VNL92_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93276.1"
                     /translation="MADYYYERETRTPHSESYLIDDEEGQHIGRVDIHYVPSGPVHAT
                     LCVGEGMTEEDLEDLIGDVDERLVMTADPFREDFVVSVWRGRSAGVYSEESLDLEEDL
                     DDGQNGHR"
     gene            complement(3020..>3553)
                     /locus_tag="VNL92_00765"
     CDS             complement(3020..>3553)
                     /locus_tag="VNL92_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HWO93277.1"
                     /translation="ISDSSGGVYNPGGIEVAAARAAKRQGPLAQTQGLGDPITNEELL
                     ALPVDILVLAAMEGAIDAKNASHVRAPLIIEGANGPISRDADAILEGMGTTVVPDILA
                     NAGGVVVSYFEWVQDRQAFWWDAEEVDQRLARILSEAFQNVLATGAARSVPLRMAALL
                     LGIGRVVEATRTRGIFP"
BASE COUNT          612 a         1105 c         1180 g          656 t
ORIGIN      
        1 cttgcttgca ggaccggacg gggctttggt cagcgacggg cggcgtcagt tccagtgatg
       61 gaacgctggc agggcggaga gctcggcgta aacctcgatc gagagatcgc tgacgtagtc
      121 cgcccagacg gcccaatcct cgcgggcgcg ctgggcctgc gggctctgca acgcggactg
      181 cagctcgctg ttggctgaga actcgaagat ggtctgccag ttgatgggag gcgtcctttc
      241 gtggtttcgt ttcacgattt gacccccaac cacccccggg atggcgagca tatgcggtag
      301 gtggatggtg aagtgccatc gccggaattc ggccatcacc gcgtcatcga cgcgggcgcg
      361 aaccacgagc accggcttcc cgttcgcaaa catttccctc cccggtcagt cgcagacgtt
      421 ctctgggtcg aaccggcaca gattcacgtc gacgaagcgc tccactcgct ccatgtccag
      481 ttcatcgaag gcgtcgaggc gcgtccagga gatgagcgcg atgcgatgct ccatgccggg
      541 gtacggagcc atgagaagag catcgccgtc cgagaccttc ccctgcacgt aggcgctgag
      601 ctcgctcacg atgtccggac agtcgtcggt acagttgtag aggacagcga ctccgccgtg
      661 ctccaagttg tggaggatct tctccttcgg aatgggatcg gcggagacac cccaaggggc
      721 gggtacgggg tcatgtggac ccgacgtagg cgggttcgag ttgtactcgt cgtagggctg
      781 gttagcctgg aggtggctgc ggccgaggtc ctcgaagaat tggcctggga tatcggcgct
      841 gctgtcctca ttggcagcgg tgggctcgtt gatctggtcg acgatgacgt aggcgaggac
      901 cccgacgacg gcgatgacga gcaaggcaac gatgatgttg cgggggcgga atacgtcgac
      961 gcctccgccc acggcaacgg gtacgtccga gggtcggcgc ctcgctcctg ggccctcccg
     1021 ccgccgtgcc tgacgattcg accgttcgct ccgattcaat gcccacctcc gagtatctcc
     1081 gttgcgtgcg ttggggcgcc ggtccgcaat cggggaatcg ctgtgcgaag aaacgcaagc
     1141 cgtgatccga gtatagtaga cacccgctgt attctgccaa cacgtgacgc gtctcgacag
     1201 gctcgcgttt ctcgacagtc gattctgctg atgatacgct tcctcgtgag tacaggatcg
     1261 atggctggcc gatcaagttc tttggctctt gccagtgttg cgctcgtaag ggggcatgat
     1321 gcaagcgatc tcgtcggagc ttgagacgaa ggcgcgggcc gtcaaggcgg cggcgcctgc
     1381 gctggcccgc gcctcgaccg acctcaagaa cgctgcgctg cttgccatcg cccgcaacat
     1441 cgaggagcgg gtagccgatg tgctgaccgc caacgcggcc gattgcgccg attcccgcgc
     1501 tgccggccta tcggatgcca tgttggaccg gctgacgctg accgagggcc gtctgcgctc
     1561 gcttgccgaa gacgttcgta aggtggccgc gctgcccgat ccggtcggcg agatgttcga
     1621 tgttcgaatg ctgccgaacg gcctgcaggt aggccgccgg cgtgttccct tgggcgtcat
     1681 cgccgtcatc tacgaaagcc ggccgaacgt cacggtggat gtcgcttcgc ttgcgctgaa
     1741 gtcgggcaat gctgcactcc tccgcggggg tcgcgaggcc atccgctcga acatcgctct
     1801 cgcggccgtc gtgagggagg ccatcgccga ggtcgggttg cccgagcaaa cggtggagct
     1861 tgtagagtcg accgaccggg cgatcgtcgg ggagcttctc ggcatgcgtg agaccatcga
     1921 tatcgtagtt ccgcgcgggg gcgccgactt catacgttac gtgatggaca acgctacgat
     1981 gccggtcatt gctcacgggg atgccgtgag ccatacctat gttgatgccg ctgcggatgt
     2041 catgaaggct gtcgagatcg tggtcaatgc gaaatgccgt cgctggggca tctgtaacgc
     2101 actggacacg ctgctcgtgc acgaggccat tgcgacgccc ttcctgcagg cgctggcgcc
     2161 gcgctgggcc gagtacggca tcgaggcccg ctgcgacgaa cgcgccggcg cggtcctggc
     2221 ccacaccccg ctcgcgggcc gggtaaggcc agtactgcct gaggattacg gattcgagtt
     2281 tctggctcct gtggccgccg tccgagtcgt cgactcgctc gaggaggcga tggcccacat
     2341 cgagcttcac agctcacggc attcggaggc aattgtcacg gagaattaca ctgccgcgca
     2401 gcggttcctc gaggaggtcg attcggcgtg tgtctacgtg aatgcgagca cgcagttcac
     2461 cgacggcgca cagttcggcc tgggcgctga gatcggcatt tccacgcaga agatgcacgc
     2521 tcgcgggccg atgggcctac gggagctcac gtcatataag tggattatcc ttgggtcggg
     2581 ccagattcga ccgtagcgga agaagccgac accgacttcc gattgggccg catcagcaaa
     2641 agggactgtc gctccctggg agatcgccga gagcatggcc gactactact acgaacggga
     2701 aacacgcacc ccccactccg agagctatct catcgacgac gaggaggggc agcacatcgg
     2761 ccgcgtcgac attcattacg ttccgtcggg cccggtgcac gcgaccttgt gcgttggcga
     2821 ggggatgacc gaggaggacc tcgaggacct catcggcgac gtcgacgaac ggctcgtcat
     2881 gacggcagac ccgttccggg aggacttcgt cgtcagtgtg tggcgcggtc gatcggcggg
     2941 agtgtactcg gaggaatcgc tcgacctgga ggaagacctc gacgatggcc aaaacggcca
     3001 tcgttgaacc cgtagcgagc tacgggaaga tcccgcgagt acgcgtcgct tccacgacgc
     3061 gcccgatccc aaggagcagc gccgccatcc gcaagggcac gctcctggcg gcgcccgtgg
     3121 caagcacgtt ctgaaacgcc tcgctcagga tgcgcgcgag cctctggtcg acctcctccg
     3181 cgtcccacca gaacgcctgc cggtcttgca cccactcgaa gtaggagacg accactccgc
     3241 ccgcgttggc gaggatgtcg ggcacgaccg ttgtgcccat gccctcgagg atcgcatcgg
     3301 cgtcgcggct gatgggccca ttcgcgccct cgatgatcaa cggcgcccgg acatgcgagg
     3361 cgttcttcgc atcgatcgct ccctccatcg ccgcgaggac gaggatatct accgggaggg
     3421 cgagcagctc ctcgttcgta atggggtcac ccagtccctg cgtttgggcc agtgggcctt
     3481 gtcgctttgc tgcccgcgcc gcggcgacct cgatgccccc tggattgtag acgccgcccg
     3541 aggagtcgct gat
//
LOCUS       DATMBB010000038         2530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_116615, whole genome shotgun sequence.
ACCESSION   DATMBB010000038 DATMBB010000000
VERSION     DATMBB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116615"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VNL92_00770"
     CDS             <1..780
                     /locus_tag="VNL92_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93278.1"
                     /translation="NLDDPLPVQYLDWVGDIARGDFGESLLTTQDIGKEIRKRFPVTA
                     ELAVLAVLFSIVIGLPAGVIAAIRQDTPLDYVVRVLSILGLAVPGFWLASLIITLPAI
                     WWQTGPPLGHTPFLDDPIENLKAYATPAILLGLAQGALLMRLTRSGLIEVMRQDYIRT
                     AWSKGLRERVVILRHAMRNALIPVITVMGLQLTVLLGGTVIQEQIFLLPGLGFYLLQA
                     ISLRDYPVLQTVILLLTVIYVVVNLIVDLTYGYLDPRIRYS"
     gene            831..1775
                     /locus_tag="VNL92_00775"
     CDS             831..1775
                     /locus_tag="VNL92_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93279.1"
                     /translation="MAAVQTTAAEREEWLLPRRRRIPVISPVWKFIRQKPLAAFGGLI
                     IVTVLFIAFFAPLVTWHSPTDVPVQFDLEGGSGGGLNRLADPSTDHIMGTDDKGRDVW
                     ARVAYGSRISMQVGFAAVAIAVSVGTIIGLVSGYFGGWTDTLVQRLVDAMLAFPPLIL
                     ALAIVGLLGPETSNLIISIGIIASPSIARVVRGAVLSAKENQYVEAARSVGANPTRIM
                     FRHVLPNVTAPIIIIGTALLGTAILVEASLAFLGFGTPPPDPSWGRMLSGEGRQYMVA
                     GAWWIAVFPGLAITISVLGFNLLGDGLRDVLDPRLRGV"
     gene            1987..>2530
                     /locus_tag="VNL92_00780"
     CDS             1987..>2530
                     /locus_tag="VNL92_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009804602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93280.1"
                     /translation="MESDNYWKRVARRRASRRTVLRASAVGASGAAAYAAVGCGDDDD
                     DDDDGGGTTTTGGGTADTSQLDPQFQNARYGGILQYGINDPPQTLDFHTQEPPGSHYC
                     AHPAYNGLIYRIEDEPGHFSELRGELAETWEQPDEQTIVLNIRQGVKWQNVDPMGGRD
                     FTAENVVYNIERMRGEHPAGN"
BASE COUNT          441 a          828 c          769 g          492 t
ORIGIN      
        1 aacctcgacg accccttacc cgtgcagtac ctcgactggg taggcgatat cgcccgcggg
       61 gacttcggcg aatcgctcct gaccacgcag gacatcggca aggaaatccg caagcgcttt
      121 ccggtgacgg ctgagttggc ggtcctcgcc gtcctcttct cgatcgtcat tgggctaccc
      181 gccggcgtga tcgcggcaat acggcaggac actccgctcg attacgtggt ccgagtgctc
      241 agcatcttag gattggccgt tccgggcttc tggctggcat cgctgatcat caccttgccc
      301 gcaatctggt ggcaaacagg accgcccctc ggtcacacgc catttctcga tgaccccatc
      361 gagaacctga aggcctacgc cacgccggcg attctgctcg gcttggcaca gggtgcgctc
      421 ctgatgcgtc tcacgaggtc cggcctcatc gaggtcatgc ggcaggacta catccgcacg
      481 gcgtggtcga aggggcttcg cgaacgtgtg gttatcctgc gccacgccat gcgcaacgcg
      541 ctcatcccgg tcatcaccgt gatgggcctg cagctcaccg tgctgcttgg tggcaccgtc
      601 atccaggagc agatcttcct tctcccgggg ctcggattct acttgctcca ggccatttcc
      661 cttcgcgatt atccggttct gcagaccgtc atcttgcttc tcacggtaat ctacgtggta
      721 gtgaatctca tcgttgatct cacgtacggg tatctcgacc cgcgtattcg ttacagctaa
      781 ggtgaatcgg gccatctcgc ccgagcgtac actcattcga gggggaatca atggcggcgg
      841 ttcagaccac agctgcagaa cgtgaggaat ggctgctgcc gcgcaggcgc aggattccag
      901 tcatttcacc cgtctggaag ttcattcggc aaaagccgct cgcagcattc ggcgggctca
      961 tcatcgtgac cgtgctgttc atcgccttct tcgcgccgct cgtgacgtgg cacagcccca
     1021 ccgatgtccc ggtgcagttc gaccttgaag gcgggagcgg aggcggactg aatcgcctcg
     1081 ccgatccatc caccgatcac atcatgggaa cggacgacaa gggccgcgac gtatgggctc
     1141 gcgtggcgta tggctcgcgc atctctatgc aggtcggctt tgccgccgtc gcgattgccg
     1201 tgtcggtagg caccatcatc gggctcgtat ccggctactt cggcggctgg accgacaccc
     1261 tcgtacagcg cctcgtcgat gccatgctcg ccttcccccc gctcatcctt gccctggcta
     1321 tcgtgggcct gctggggccg gagacgagca acctcatcat atcgatcggc atcatcgcct
     1381 ccccatcgat tgcgcgcgtg gtacgcggcg ccgtgctttc ggcgaaagag aaccaatacg
     1441 tcgaggcggc gcgcagcgtc ggggcgaacc ccacgcggat catgttccgc cacgttctac
     1501 ccaacgtcac ggcgcccatc atcatcatcg gcacggcgct cctcggcacc gcgatcctcg
     1561 tcgaggcctc actcgcgttc ctcggattcg gcaccccgcc gcccgaccca tcctgggggc
     1621 gcatgctctc cggcgaaggc cgccagtaca tggttgccgg cgcttggtgg atcgctgtgt
     1681 tccccgggct cgctatcacg atcagcgtgc tcgggttcaa cctcttgggc gatggattgc
     1741 gcgacgttct cgatcctcgg ctacgaggcg tctagatccc agccgttgcc agttggacac
     1801 ttctgtgtct cgtgggcgag tcgggtacca gtccgccatc cgcgggccgt gctcacgcgt
     1861 atcttgatgt ggaggcgcag gtaggtgagg gtgcacgttg acctccgctc ggcgccgggt
     1921 gtagcgtagg acgtcgaatc taggtcgcct tcgaggccgt atggaaccta gcgaggggag
     1981 taaacgttgg aatcggacaa ctattggaag cgagtggcgc ggcgccgagc atcgcggcgc
     2041 accgtgttga gagcgagcgc cgtcggcgct agcggcgccg cggcatatgc tgcggtcggc
     2101 tgtggcgacg acgacgacga tgacgacgat ggcgggggaa ccacgactac tgggggagga
     2161 acggccgaca cgtcgcagtt ggacccgcag ttccaaaatg ctcgctatgg cggcatcctc
     2221 cagtatggca tcaacgaccc tccccaaacg ctcgacttcc acacgcaaga gccgcccggc
     2281 tctcactact gcgcgcaccc ggcctacaac ggcctcatct accgaatcga agacgagcct
     2341 ggtcacttct ccgagctgcg aggcgagctg gcggagacgt gggagcagcc cgacgagcag
     2401 acgatcgtcc tcaacatccg tcagggcgtg aagtggcaaa acgtcgaccc catgggcggg
     2461 cgcgatttca ccgccgaaaa tgtcgtctac aacatcgaac gcatgcgagg ggagcatccc
     2521 gcagggaacc
//
LOCUS       DATMBB010000039         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_190760, whole genome shotgun sequence.
ACCESSION   DATMBB010000039 DATMBB010000000
VERSION     DATMBB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190760"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VNL92_00785"
     CDS             <1..1170
                     /locus_tag="VNL92_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWO93281.1"
                     /translation="SLAYQADVVAIDPSLDVAVLRIAANREGEEIAPEEAAIGAQLVL
                     GSDEAPRNAVGLGYAARDSGQPAGVRLEFSAGLDGEGEWLEAPAVLPPGYAGAPVLDD
                     AGAIVGLVAIPEPGDEAVFVRRISDARPMIEAALRGQELLLSGRVRLPPPERSESDFP
                     RDLEIGGITFYTDLTPDSDPVGPTDHFPEGTRRIVYAFEYAAMRDGLPWLDEWRINGE
                     LDPQLSEPRPPWNRGPDGTLVSSINEPNGFSSGEYEVRVIVDSVLMASARVDVGDVPA
                     VPNLKSLVLAPAKGDDGQPVGVGERLESASTLYGFFDYEGMDAVSNWGYVWYHGDDIV
                     AERDGLPWRGGGAGSDWWVAYYEPDGSALQEGEYTLTLLLDGEPSATARVTIGAS"
     gene            1197..1916
                     /gene="amaP"
                     /locus_tag="VNL92_00790"
     CDS             1197..1916
                     /gene="amaP"
                     /locus_tag="VNL92_00790"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HWO93282.1"
                     /translation="MQFFNRALITVLSVLLAVALFALVIVIWADPEGGVDRLGDLTSY
                     LDERTDDDLARALISLGAGTVGLLLLMLVVAEVAPAGTDRIRLSQAADGVTELSATSV
                     NRRLESNLRRLPDVDEARAHVRAHQRGVSVRLELWLNPHANLAAASDEACRLVKEDLE
                     ERVGVPLVSAPNVYLRYSEEPGEAARGGPWYESVDRPPESQRRAEPVPGNGDADPPLH
                     DAEPKEPEVADSEPVEERHAP"
     gene            1906..>2329
                     /locus_tag="VNL92_00795"
     CDS             1906..>2329
                     /locus_tag="VNL92_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWO93283.1"
                     /translation="MPPDPGPKARKERLPALLESLLFVADEPVSLGELAEALGVSARA
                     VSSALDELAAASAERGVRVQRAGDRVQLVTNPEAAPFVERFLGRDIPQRLSVAALETL
                     AIIAYRQPVTRAVVDQIRGVNSDRALSTLRARGLIEEVG"
BASE COUNT          353 a          750 c          839 g          387 t
ORIGIN      
        1 tcgctcgcct accaggccga cgtcgtcgcg atcgatcctt cgctcgatgt cgcggtgctg
       61 cgcatcgctg cgaatcgcga gggcgaagag atcgcgccgg aggaagcggc catcggggcg
      121 cagcttgtgc tcgggagcga cgaggcgcca cggaacgccg tcgggttggg gtacgccgcg
      181 cgcgattcgg ggcagccggc cggcgtgagg ctcgagttct cggccggtct ggacggcgag
      241 ggcgagtggc tcgaggcgcc ggcggtcttg ccgccggggt acgcaggcgc accggtgctc
      301 gatgacgcag gggccatcgt gggcctcgtg gcgattccgg agccggggga cgaggccgtg
      361 ttcgtacggc gcatctcgga cgcacgtccg atgattgagg cggccctgcg cggccaggag
      421 ctgcttctca gcgggcgcgt ccggcttccg ccaccggagc gcagtgagag cgacttcccg
      481 cgcgacctgg agatcggcgg catcacgttc tataccgacc tcacgcccga tagcgacccc
      541 gtaggcccaa ccgaccactt ccccgaaggc acgaggcgca tcgtctacgc cttcgagtat
      601 gcggccatgc gggacggtct gccgtggctc gacgagtggc gcatcaatgg ggaactggac
      661 ccgcagctca gcgagccgag gccaccgtgg aatcgtgggc cagacgggac gttggtatcg
      721 tccatcaacg aaccgaacgg cttctcctct ggagagtacg aagttcgcgt catcgtggac
      781 agtgtgctca tggcctcggc ccgagtggat gttggcgatg tgccggcggt cccgaacctc
      841 aagtccctcg tgctggcgcc tgccaagggg gacgatgggc agcctgtcgg agtcggcgag
      901 cggctcgaaa gcgcttcgac gctgtacggc ttcttcgact acgaaggtat ggatgccgtc
      961 tcgaactggg gatacgtctg gtatcatggc gacgatatcg tggccgaacg ggatggactg
     1021 ccgtggcggg gcggtggcgc cgggtccgat tggtgggtcg cgtactatga gccggacggc
     1081 tctgctttgc aggaaggcga atacacgcta acgctgctcc tggatggcga gccatcagcg
     1141 actgcgcgag tgaccatcgg ggcgagctag cgcgccgcac ccatcggggg acaacgatgc
     1201 agttcttcaa ccgggccctc atcactgtcc tctcggttct cctcgccgtg gcgctcttcg
     1261 cgctcgtcat cgtcatctgg gcagacccgg agggcggggt cgaccgtctg ggggacctca
     1321 cctcgtacct cgacgagcgt accgacgacg acctcgctcg ggcgctcatc tcgcttgggg
     1381 cgggaacggt aggcctgctt ctgctgatgc tcgttgtcgc cgaggtcgcg ccggccggga
     1441 cggaccggat ccggctttcc caggcggccg atggcgtcac ggagctgtcg gccacgtcgg
     1501 tcaatcgccg tctggagagc aacctccggc ggctgcccga cgtcgatgag gcacgagcgc
     1561 atgttcgcgc gcatcagcgt ggcgtgtcgg tgcgcttgga gctgtggctg aatccgcatg
     1621 caaatctcgc tgccgccagc gacgaggcct gcaggctcgt gaaggaggac ctcgaggagc
     1681 gcgtcggcgt acccctcgtg agtgcgccga acgtctatct ccgatactcc gaggaaccgg
     1741 gcgaggcggc gcgcggcgga ccttggtacg aatcggtgga ccggccccca gagagtcaac
     1801 gccgtgccga accggtcccc ggcaacggcg atgccgaccc gcccctacac gacgcggagc
     1861 cgaaggagcc cgaggtggcg gacagcgagc ccgtggagga gcgccatgcc ccctgaccca
     1921 ggtcctaagg cgcggaagga acgcttgccc gccctgctgg agagcctgct gttcgtggcc
     1981 gacgagccgg tgagcctggg cgagcttgcc gaggcgctcg gcgtctcggc aagagccgtg
     2041 agttctgcac tcgatgagct cgctgccgcc tccgcggagc gtggcgtccg cgtgcagcgc
     2101 gccggcgatc gtgtgcagct ggtaacgaat ccggaagctg cgcctttcgt ggagcgcttc
     2161 ctggggcggg acattccgca gcgcctctcc gtcgcggcgc ttgaaacgct ggcgatcatc
     2221 gcttaccggc agccggtgac ccgcgccgtc gtcgatcaga tccgtggtgt caactcggac
     2281 cgcgccttgt cgacgctgcg agctcgtggc ctcatcgagg aggtcggca
//
LOCUS       DATMBB010000040         2448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_550544, whole genome shotgun sequence.
ACCESSION   DATMBB010000040 DATMBB010000000
VERSION     DATMBB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550544"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VNL92_00800"
     CDS             complement(<1..1286)
                     /locus_tag="VNL92_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWO93284.1"
                     /translation="MNEALLPIIDSFAHLRVLVIGDAMLDIYLRGSTERLCREAPVPI
                     VALGDRSEVPGGAANVAANVRSLSAEVRLLATIGDDGEGIALAHALTAAGVSNDDLLR
                     VPGRRTLTKTRIVSGGQMVLRVDQGDTGALPPEAQNELVGRLLEAVAWADAVILSDYG
                     YGVACGPVLEALKDVRRRSDLVLSADGKDPTRYRALAPTAVKPNYAEAMALLDRSAEA
                     GNRAERILRQQNRILEQTGAQVVAVTLDREGAVLLERGGTAYRTYAHPVADSRAAGAG
                     DTFVAALTLALAAGAQPTSAGEIASAAANIVVTKDGTAVIDERELRESLLGGNKLLAG
                     RGELARRAQLLRAQGKRLVFTNGCFDIIHRGHVSYLSAAKALGDVLVVGVNSDESVAR
                     LKGPGRPINCLEDRLQILAALSCVDYIVPFGEDSPVT"
     gene            complement(1283..1912)
                     /locus_tag="VNL92_00805"
     CDS             complement(1283..1912)
                     /locus_tag="VNL92_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWO93285.1"
                     /translation="MRAKLREQGSPKRAVFIDKDGTLVVDVPYNVDPALIELTPGAAS
                     AMRTLAGAGYELFVVTNQSGVAKGLFPEAALGAVQDRIAALLADAGVALSGFYYCPHD
                     PARAPCLCRKPQPGLIRHAAREHGVDLQRSWMIGDILADVEAGNRAGCRSALVDGGNV
                     TWAELTEANVPTIVARNLEDAARQILRIDVHRSQQPAPAVLGRPRGGAP"
     gene            complement(1909..>2448)
                     /locus_tag="VNL92_00810"
     CDS             complement(1909..>2448)
                     /locus_tag="VNL92_00810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO93286.1"
                     /translation="DVSEEDFVRTVVSSVLNDLGRVDVLINNAGVDVTVPIDDLMVAD
                     WDRILGVNLRGPFLMTKEVLPSMRELGQGHIVNIVSTAAKRSWPEAAAYHASKWGLLG
                     FSHALHAELRASNIKVTAIIAGGMRTPFLLDRFPEIDTTTLQDPRAVAETVRFAITQP
                     EGTVIPELMVLPLRETSWP"
BASE COUNT          434 a          807 c          770 g          437 t
ORIGIN      
        1 gtaaccggcg agtcttcgcc gaagggcacg atatagtcga cacagctcag cgcggcgagg
       61 atctgcaacc ggtcctcgag gcagttgatg gggcgacctg gccccttcag tcgagctacg
      121 ctctcgtcac tgttaacccc aacgacgagc acatcgccga gcgcctttgc ggcactcagg
      181 tacgacacgt gaccacgatg gatgatgtcg aagcagccgt tcgtgaagac gaggcgcttg
      241 ccctgcgcgc ggaggagctg ggcccgtcga gcgagctctc cgcgaccggc cagcagcttg
      301 ttgccgccca acagcgattc gcgcaactcg cgctcgtcga tgaccgcagt gccatccttc
      361 gttacaacga tgtttgccgc tgcggaggcg atctctccgg ccgatgtggg ttgcgcccca
      421 gcggcgaggg caagggtcag cgcagcgacg aacgtgtcgc cagctcccgc ggcgcgagaa
      481 tcggcgacgg gatgagcata cgtgcgatag gccgtcccgc cgcgctccag cagcacagcg
      541 ccctcgcgat cgagcgtgac agccacgacc tgggctccgg tttgctcgag aatgcggttc
      601 tgctggcgca atatccgctc ggcgcggttg ccggcctccg ccgatcggtc gagcagggcc
      661 atggcttcag cgtagttggg cttgaccgcg gtgggtgcga gcgcacgata tcgcgttggg
      721 tccttgccat ccgccgacag aaccaggtcg gatcgtcgcc tgacatcctt caaagcttcg
      781 agtactggcc cgcaggccac cccgtagcca tagtcggaga ggatgacggc atcggcccag
      841 gccacggcct cgagcagtct gccaacgagc tcgttctgcg cctcgggcgg tagagcgccc
      901 gtatcgcctt gatccacccg caacaccatc tgaccgccgg agacgatcct ggtcttcgtg
      961 agcgtccggc gccctgggac gcgaagtagg tcatcgttcg acacacctgc agcggtgagg
     1021 gcatgggcaa gggctattcc ctcgccgtcg tcgccgatag tcgccagtag cctcacctct
     1081 gcagacagcg atcgaacgtt cgctgccacg ttcgctgcgc cgccggggac ttcgctccga
     1141 tcgcccagag cgacaatcgg caccggcgct tcccggcata accgctcggt actgccgcga
     1201 aggtagatgt cgagcatggc gtcgccaatg accagcactc tcaaatgggc aaacgaatcg
     1261 atgatgggca agagtgcctc attcatggtg cacctccacg aggtcggccg aggactgcgg
     1321 gcgccggctg ctgcgaacgg tgcacatcga tccggaggat ctggcgggct gcatcctcca
     1381 gattcctcgc gacgatggtt ggcacattcg cttccgtcaa ctcggcccac gttacgttgc
     1441 caccgtcgac caacgcgctg cggcagcccg cccggttccc cgcttccaca tcggcgagaa
     1501 tgtcgccgat catccacgat cgctggaggt cgacgccgtg ttcgcgggct gcatgccgaa
     1561 tcaagcctgg ctggggtttc ctgcacagac agggagcgcg cgcggggtcg tgaggacagt
     1621 agtagaagcc agagagcgcc acccccgcat cggctagcaa tgcggcgatc cgatcctgta
     1681 ccgcacctag cgccgcttcg gggaacagcc ccttcgctac gccggattga ttcgtaacga
     1741 cgaagagctc gtagccggct ccagcgagcg ttcgcatcgc gctcgcggcg ccgggtgtca
     1801 gctcgatgag cgcggggtcg acgttgtacg gcacatcgac cacgagcgtt ccatccttat
     1861 cgataaacac ggcgcgcttg ggcgaccctt gttcgcgtag cttcgccctc atggccagga
     1921 cgtctccctt aacgggagga ccatgagctc cggtatcacg gttccctccg gctgcgtgat
     1981 cgcgaatcgc acggtctccg cgacggcgcg gggatcctgg agtgtcgtgg tatcgatttc
     2041 cggaaagcga tcgagcagga agggtgtccg catgcctccc gcgatgatcg ctgtgacctt
     2101 gatgttgctt gccctcaact ccgcatgcag ggcgtggctg aatcccagga gaccccactt
     2161 gctggcgtgg tacgctgctg cctccggcca cgatcgcttc gctgcggtcg agacgatgtt
     2221 cacgatgtgg ccctgcccca gctcgcgcat cgaaggcagt acttccttcg tcatgaggaa
     2281 cggaccacgc aagttgaccc cgagaatgcg gtcccagtcc gcgaccatga ggtcatcgat
     2341 aggcaccgtc acatcgacgc cggcgttgtt gatgagaacg tcgacgcgac ccaggtcgtt
     2401 gagtacggac gacacaaccg tccgcacgaa gtcttcctcg gacacgtc
//
LOCUS       DATMBB010000041         3986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_115637, whole genome shotgun sequence.
ACCESSION   DATMBB010000041 DATMBB010000000
VERSION     DATMBB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115637"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VNL92_00815"
     CDS             complement(<1..391)
                     /locus_tag="VNL92_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWO93287.1"
                     /translation="MDRTHAPYVELHLHTNWSLLEGASFVDEIVGAAASMGYRALALT
                     DHDNLFGAMEFARTARAAGILPITGAELTMEDGSHLTLLAETRAGYGNLCRLISIANG
                     HGQATQQLKEERRLEPRLPFAALAEHTS"
     gene            complement(631..1830)
                     /locus_tag="VNL92_00820"
     CDS             complement(631..1830)
                     /locus_tag="VNL92_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWO93288.1"
                     /translation="MAGNSTRQVACLLISHFAMACELAERPELRGHPVVLGGGDAGPR
                     VVDLTDEALRYGIDPGISLREAIALCPALTVIEPRPALYDDMFDDTLDALHQVSPIVE
                     RGGLGVTYADLTGLERLYGPRLPEALLVCAPPMLAPRLGIAWGKFPALAAAAFAEPGT
                     PLLVPRSETAGFLSPQSVDLLPLPSDAHRRLRMLDIRTVGAFAALPRSAVQAQFGATG
                     ALAWDLAHGNDDRPLDPEPHTERIVEVLEFAPPLTGRDAVLMALEQMLVRALRQPGAR
                     NRFARGLFIRAATERGRSWSRRYTLKEPSSDRVRLAAILRTLVEYAEFPGALLELRLE
                     LTDLTREGGQQAQLFRERTRVREELETTLRQLKTRYGHCPVGRVVEVEPWSRVPERRM
                     ALVDYDP"
     gene            1897..2715
                     /locus_tag="VNL92_00825"
     CDS             1897..2715
                     /locus_tag="VNL92_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWO93289.1"
                     /translation="MPSIVSLTTDFGYSDAYVAAVKGVILSICPEVDLVDVTHEIPPG
                     AIEPAVFITGCAWPYFPPDAIHVVVVDPGVGTDRRAIALRTRSGIFIGPDNGALSAAL
                     SDDVRDLARSHDGLATLPPQVEARELTNKAFRRPSPSPTFHGRDIFAPAAAHLARGAP
                     FDQLGPAVHEVVALPPFRAGRLADGTLVGRVMHVDRFGNLITDVRGEDLAGTGVTVEL
                     RGTVIAGLARTFAEGHGLLAYVGSAGYLEIAKNGGRAADELRAAPGDAVLVRLS"
     gene            complement(2712..3059)
                     /locus_tag="VNL92_00830"
     CDS             complement(2712..3059)
                     /locus_tag="VNL92_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HWO93290.1"
                     /translation="MTTATQRDARLQQCIDNCTECHNVCIETVQYCLEMGGKHAEAQH
                     IRRLLDCAEICALSADYMLRGSDMHRRTCAVCADACERCAQDCERFGDDEMMRRCAEI
                     CRACAQSCREMAR"
     gene            complement(3279..>3986)
                     /locus_tag="VNL92_00835"
     CDS             complement(3279..>3986)
                     /locus_tag="VNL92_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93291.1"
                     /translation="GVPDPTAPLPWGIPRLMATLKEETGAELEFHGHNDFGLATANAM
                     MAWRYGCTKINCAFGGLGERTGNTAVEQMVAAMIRLYGDPGFNLGALREIAEFIEHEV
                     SQLSERAPIVGPVFSTQAGLHQTGVARQEDAPGGFIYLPYDPELVGAHQEELSLVGAV
                     SGMDGIAAVLNYGLRQRGKDAHFTNANRVVKLIYDRVQAAYDGEYDAASGQYKNVRRT
                     FFTPEELVAMAEELGAV"
BASE COUNT          653 a         1287 c         1345 g          701 t
ORIGIN      
        1 cgctcgtatg ctcggccagc gcggcgaagg ggaggcgtgg ctccagccgc cgttcctcct
       61 tcaactgttg ggtcgcttgg ccgtgaccgt tggcgatgct gatcaatcgg cagaggttgc
      121 cgtagccggc ccgcgtctcg gcgaggagcg tgaggtggga gccgtcctcc atggtcagct
      181 ctgccccagt gatgggcagg atgccggcag cgcgggcggt ccgtgcgaac tccatggccc
      241 cgaagaggtt gtcgtgatcc gtgagggcga gagcgcggta tcccatggag gctgctgccc
      301 cgacgatctc gtcgacgaag gaggcgcctt ccaggagcga ccagttggta tggaggtgca
      361 gctcgacgta gggggcgtga gtcctatcca tgtctgccac tcaatagggc tgttcgtacc
      421 attgctcgcc ctcggctcgt aagtctcgat acagcaccat cgttcttccg ctcgccagac
      481 gcaggcgcca gtatcgccgg gcgatcggcg tcgctcgcca ccatccttca tcgacgcgcc
      541 atgtttcgtc gatctcggac acacgttcgc cccggacggc gatgggcgtg ccctcggcgt
      601 tcgcgaccgt ggatacggaa cgaggctcgt ttaggggtcg taatcgacga gggccatgcg
      661 gcgttcgggc acgcggctcc atggctccac ctccactacc cgccccacgg ggcagtgtcc
      721 gtagcgggtt ttcaattgcc gcagtgtcgt ttccagctcc tcgcggacgc gcgtccgctc
      781 tcgaaacaac tgcgcctgtt ggccaccctc gcgggtcagg tccgtcagct ccagtcgcaa
      841 ttcgagcagg gcgcccggaa actccgcgta ctcgaccagg gttcggagaa tcgccgccag
      901 ccgaacgcga tcgctcgagg gttccttgag ggtgtaccgg cgcgaccaag accgtccgcg
      961 ctcagtagcc gcgcgaatga agagtccccg agcgaaccgg ttgcgggcgc cgggttgacg
     1021 gagggcgcgg accagcatct gttcgagagc catgaggacg gcgtcgcggc cggtcagcgg
     1081 cggcgcgaac tcgaggactt cgacgatgcg ttcggtatgg ggttccggat cgaggggccg
     1141 atcgtcgttt ccgtgagcaa ggtcccaagc gagcgctccg gtggcgccga actgcgcttg
     1201 taccgcgctt ctgggcagcg cggcgaacgc accaaccgtg cggatgtcga gcatacgtag
     1261 ccggcggtgg gcatcggacg gaagcgggag cagatccacg ctctggggcg agagaaagcc
     1321 ggctgtttcg ctgcgaggga cgagcagcgg agttcccggt tcggcgaacg cggcggcggc
     1381 gagggccgga aacttgcccc aggcgatgcc aaggcgtgga gccagcattg ggggtgcaca
     1441 gacaaggagc gcctccggca ggcgtggtcc gtaaagtcgc tccagccccg tcagatcggc
     1501 gtacgtaacg ccgaggcctc ctcgttcgac gattggactt acctggtgga gcgcgtcgag
     1561 cgtatcgtcg aacatatcgt cgtagagggc ggggcgaggt tcgatgacgg taagggcggg
     1621 gcagagggcg atcgcttcgc gcagggagat gccgggatcg atgccatagc gaagggcttc
     1681 gtcggtcagg tcgaccacgc gaggacctgc atcgccgccg ccgagcacca cgggatgtcc
     1741 tcgtaactcg ggccgctcgg cgagctcgca cgccatggcg aaatgagaga tgagcaggca
     1801 agcgacctgc ctcgtggaat tccccgccat gcaggcacta tactagaaca gatgtacgac
     1861 atcaagggca gcgaagggac tcgatatact tttgccatgc cgtctatcgt ctcgctgacc
     1921 acggatttcg gatactccga tgcctatgtc gccgccgtca aaggcgtcat cctctccatt
     1981 tgtcccgagg tcgatctcgt cgacgtcacg cacgagatcc ctccaggcgc tatcgagccg
     2041 gccgtattca tcacgggttg cgcgtggccg tatttcccac ccgatgccat tcacgtcgtc
     2101 gtcgtcgacc cgggtgtcgg gacagatcgc cgggcgatcg cccttcgcac gcgctccggc
     2161 atcttcatcg gcccggacaa cggcgcgcta tcggccgcgc tgtccgatga tgtccgcgat
     2221 cttgcgagga gccacgatgg cctcgccacg ctgccgcctc aggtcgaggc ccgagagctg
     2281 acgaacaagg ccttccgccg cccctcccca agcccgacct tccatggtcg ggacatcttt
     2341 gctccagccg ccgcccacct cgcgcgcggc gcaccgttcg accagttggg accggcggtg
     2401 cacgaagtgg ttgcgctgcc cccgtttcgc gccggccggt tggccgatgg gaccctcgtg
     2461 ggccgcgtga tgcacgtcga ccggttcggc aatctcatta cggatgtgcg gggcgaagat
     2521 ctggctggca cgggagtcac ggtggagcta cggggcacgg tcatcgcggg cctcgcgcgg
     2581 accttcgcgg agggccacgg gctgctcgct tacgtgggaa gcgcggggta cctggagata
     2641 gcgaaaaacg ggggccgcgc tgcagacgag ctgcgcgccg cccccggtga tgctgtcctc
     2701 gttcgcctca gctagcgagc catctctcgg cacgactgag cgcaggcgcg gcatatctcg
     2761 gcacaacggc gcatcatctc atcgtcgccg aagcgctcgc agtcctgggc gcaccgctcg
     2821 caggcgtcgg cgcaaacggc gcacgttcgc cgatgcatgt ccgacccgcg cagcatgtaa
     2881 tcggcgctca gcgcgcagat ctctgcacag tcgagcagcc ggcggatgtg ctgcgcctcc
     2941 gcgtgtttgc cccccatctc gagacagtac tggactgttt cgatgcaaac gttgtggcat
     3001 tcagtgcagt tgtcgatgca ttgctgcaat cgagcgtcgc gttgcgtcgc cgtcgtcatt
     3061 gctcgcacct cctgcggatc tcctctccga ccgacgttcc gatgctatca aggtccaagc
     3121 aggctacaca tcaagggcat gtcaagcatc gttggggcat gtgtcaggta cgtaacacga
     3181 ccgcgggacg ttttcgccgg ccgcagggag ggcgacacga ctgcacctag gccaaggtcg
     3241 aggctaaggg acggatggag gggcggccta tgcccgcctc agacggcgcc aagctcctcg
     3301 gccatggcga cgagctcctc cggggtgaag aacgtgcggc gcacgttctt gtattgcccc
     3361 gaagcagcgt cgtactcgcc gtcgtaggcg gcctgcacgc ggtcgtagat gagcttgacg
     3421 acgcggttcg cgttcgtgaa gtgggcgtcc ttccctcgct gccggaggcc gtagttcagc
     3481 acggccgcaa tgccgtccat gcccgagacc gcgcccacca gcgagagctc ctcctgatgc
     3541 gcgccgacga gctccgggtc atacgggagg tagatgaagc cgccgggcgc gtcctcctga
     3601 cgggccacgc cggtctggtg gaggccggcc tgggtgctga agacggggcc cacgatgggc
     3661 gcccgctccg agagctgcga aacctcgtgt tcgatgaatt cggcgatttc gcgcagagca
     3721 cccagattga accccgggtc gccgtagagc cggatcatcg cggcgaccat ctgctcgaca
     3781 gccgtgttgc ccgtgcgctc gcccaggcca ccaaaggcgc agttgatctt cgtgcatccg
     3841 tagcgccacg ccatcatggc gtttgccgtc gccaagccga agtcgttgtg gccatggaac
     3901 tcgagctcgg cgcccgtttc ttccttcagc gtcgccatga ggcgagggat gccccacgga
     3961 aggggcgcgg ttgggtcggg cacgcc
//
LOCUS       DATMBB010000042         4343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_593378, whole genome shotgun sequence.
ACCESSION   DATMBB010000042 DATMBB010000000
VERSION     DATMBB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593378"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VNL92_00840"
     CDS             <1..1384
                     /locus_tag="VNL92_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HWO93292.1"
                     /translation="VRGTLERLPPNLAVMRNVNFGFQVQGWLPPGTEVEPEAASLLTV
                     LNPIDYHPAADGSVTGIPTEIATAGNFAIAPTIVAGTEDEISALNAILGSGPLTAAHI
                     DAIIALVEQGDYAGIDLDYRELDPSLGSQFTEFITALAARLHEQQRTLTITLPLPVRD
                     SESWDTGPFDWTQLGETVDRIKIPTPDDQSIYAARMDDVLTFLTEQAPPSKMLLIVSP
                     YSYVRVGEEITPVQLAEALSLATPVQIAEDESALVTGGTVTLVGTNLYRENNATGLQW
                     DDTANMVTFAYQEGDTPTTVWLENQYSIGFKLQLVTRHRLGGIAVLDVSSNGLKANIW
                     PPIRGMVEGGSLNLLKPNEQLLQPTWQIVDPNGQPVPDTALDGGMSGAVQWQIPATPG
                     TYVVTLIVSDGTIRVGQEAQLLVAEGSAPSDAATAPADQPPDAADAPVDEPTLVPGDG
                     GEPADTAVAE"
     gene            1413..1994
                     /gene="rsmD"
                     /locus_tag="VNL92_00845"
     CDS             1413..1994
                     /gene="rsmD"
                     /locus_tag="VNL92_00845"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWO93293.1"
                     /translation="MRVIAGTAKGRRLIAPRGARTRPTADRTRGAIFDMLEAAGADFT
                     SVLDLYAGTGALGIEALSRGADGCDFVEQDRNACEAIRRNLRLTGLEERSRVSCMSVS
                     AALGRLDRRYTLVLADPPYADTEAIAELDRLASVGLANGAFVVYEHSIKHDSPAHLGE
                     LPRWRQRRHGDAMISIYRAGHTGPPGDGQWKEE"
     gene            1994..2476
                     /gene="coaD"
                     /locus_tag="VNL92_00850"
     CDS             1994..2476
                     /gene="coaD"
                     /locus_tag="VNL92_00850"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWO93294.1"
                     /translation="MTVALYPGKFDPVTNGHLDIVRRASSLFDQLIVAVYALPNTFFT
                     ADERVDLFDRAVEDLPNVRVLKFDGLVVHFARDVGATVLVRGIRAVTGFEAEFDMALM
                     NKKMAPEIESVFLMASIEHLFVSASRIREIASLGYDPGDLVPAHVKHALLDKFQSPAH
                     "
     gene            2554..3222
                     /locus_tag="VNL92_00855"
     CDS             2554..3222
                     /locus_tag="VNL92_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93295.1"
                     /translation="MPVGGGVLIDRAQAYDLIDELRVALSDGFAPPVLAEPAANLPEL
                     EALRRAESDARSQLIRAEEEITRLRRELDARVAGQEVTRIAEERSRTTLAEAEERSRS
                     MLTEAEDEAQRIRGEAEAEAAERLAQAARVAAQQLEEADTYALQLLQRLEDQIQLFLE
                     NVRTGITQLEGKRPSDQHADRQIASPFSVGQAEDDDLDDEDEEPEEDDSRTRNRRFPW
                     LRDD"
     gene            complement(3228..3677)
                     /locus_tag="VNL92_00860"
     CDS             complement(3228..3677)
                     /locus_tag="VNL92_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWO93296.1"
                     /translation="MKARELGHVVLYVRNLERSLRFYRDLLGFEVVGSVFGGKAAALT
                     AGRTHHELLLIEVGEDATPIPEGRRVGLYHIGVKVGDGIEELREAYRQLVEAGVPIAG
                     ASDHTITKSIYVFDPDGHEVEVFTDEDPAIWRENPAAVLAPIRPLRL"
     gene            complement(3724..>4343)
                     /locus_tag="VNL92_00865"
     CDS             complement(3724..>4343)
                     /locus_tag="VNL92_00865"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWO93297.1"
                     /translation="LSACERAERSLPAMQVGQIGLGAAGLAIARSVMAYTGRTVLGAD
                     RDPSALRRLESVGGVAAEGVEEILARCDVVIATTGQANLIRPEWIRDGQFIFALSNPR
                     PEIDAAQALEAGAAVAASGRRVNNLMVYPGVCRGLLDVRARAAPLEVFQAASEALVAV
                     TPSDALLPDALDRAVHRRVSRAVARAAIAAGVARREPDPDYYDEG"
BASE COUNT          787 a         1483 c         1375 g          698 t
ORIGIN      
        1 cgtgcgggga acgctcgagc ggctgcctcc caacctcgct gtgatgcgca acgtcaactt
       61 tggcttccag gtgcaaggct ggctgccacc aggaacggag gttgaaccgg aggccgcgtc
      121 cctgctcacc gtgctcaatc cgatcgatta ccacccggcg gccgacggct cggtgaccgg
      181 catcccaacg gagatcgcga ccgcggggaa ctttgccatc gccccgacga tcgtcgcggg
      241 aaccgaggac gagatcagcg ctctgaacgc aattctgggc tccggtcccc tcacagccgc
      301 gcacatcgat gccatcatcg cccttgtgga gcaaggtgat tacgccggaa tcgatctgga
      361 ctatcgggag ctcgatccat cgctcggcag tcaattcacc gagttcatca cggcgctggc
      421 cgcgcgcctg cacgagcaac agcggacgct gaccatcacc ctgcccctcc ccgtgcgcga
      481 ttccgaaagc tgggacacgg ggccattcga ctggacgcaa ctgggcgaga cggtcgatcg
      541 gatcaagatt cccacgcccg acgatcagtc gatctatgca gcacgcatgg atgacgttct
      601 cacgttcctg acggagcagg cgcctccctc caagatgctc ctcatcgtga gcccctacag
      661 ctacgtgcgc gtcggtgaag agattacgcc cgttcagctg gccgaggccc tttcgctcgc
      721 gacgcccgtc cagatcgccg aagatgagtc cgcgcttgtc accggcggca ccgtcacact
      781 cgtcggtacc aacctgtatc gggagaacaa cgcgacgggc ctgcagtggg acgacacggc
      841 caacatggtg accttcgcgt accaggaggg tgatacaccc acgaccgtgt ggctggagaa
      901 tcagtacagc atcgggttca agctccagct cgtaaccagg caccgactcg gtggcattgc
      961 cgtactcgac gtctcgtcga acgggctcaa ggcgaacatc tggccaccta tccgcggcat
     1021 ggtcgaaggc ggaagcttga acctgctgaa gcccaacgag cagctcctcc aaccgacctg
     1081 gcaaatcgtc gatccgaacg gtcagcccgt ccctgacacg gcgctcgatg gaggcatgag
     1141 cggcgcggtc cagtggcaga tcccggcaac acctgggacc tatgtcgtca cactcatcgt
     1201 cagcgacggc accattcgcg tcggccagga ggcccagtta ctcgtagccg aaggctcagc
     1261 gccgtccgac gccgccactg ctccggccga tcagcctccc gatgctgcgg acgccccggt
     1321 ggatgagcca accctcgtcc cgggcgatgg cggcgagcct gccgacacgg cggtcgccga
     1381 atgagcgggc gggtgcctgg aacgaacgga cgatgcgtgt gatcgctgga acggcgaagg
     1441 ggcgtcgcct gattgccccg cgaggggctc gcacgcgtcc taccgcagac cgcacacggg
     1501 gcgccatctt cgacatgctc gaagcagcgg gcgcagactt caccagcgtg ctcgacctct
     1561 atgccggcac gggagccctt ggcatcgagg ccctcagccg gggggccgac ggctgcgact
     1621 tcgtggagca ggatcgcaac gcctgcgagg ccatccggcg caacttgcgc ctgacagggc
     1681 tggaggagag gagccgcgtc tcctgcatgt ccgtatccgc ggcgcttggg cggctcgacc
     1741 gtcgctatac actcgttctc gccgatccgc cgtacgctga cacggaggcc atcgccgagc
     1801 tggataggct cgcgagtgtg ggacttgcga acggagcgtt cgtggtgtac gaacattcca
     1861 taaagcacga ctcaccagca catctcggag agctgccgcg ctggcgacag cgccgccacg
     1921 gcgacgccat gatatcgatc tatcgtgccg gccacaccgg tccgccgggt gacggccagt
     1981 ggaaggaaga gtagtgacgg ttgcgctcta cccggggaaa ttcgacccag ttacgaatgg
     2041 tcacctcgac atcgtcaggc gggcctcgtc gttattcgac cagctcatcg tggccgtcta
     2101 tgcgttgccg aacacgttct tcaccgccga cgagcgcgtg gacctgttcg accgcgcggt
     2161 ggaggacttg ccgaacgtcc gcgtcctcaa attcgatggc ctcgtggttc actttgcgcg
     2221 agatgtgggc gcgaccgtgc tggtacgagg cattcgcgcc gtcacgggct tcgaggccga
     2281 gttcgacatg gccctcatga acaagaagat ggctcccgag atagagtccg tcttcctcat
     2341 ggcgagcatc gagcacctct tcgtgagcgc gagccgcata cgcgagatcg ccagcttggg
     2401 gtacgacccg ggcgacctcg tgccagccca tgtcaagcat gcactcttgg acaagtttca
     2461 gtcgccggcc cactgaggaa ccgaaggagg ccacccgtag acctctatga cgccatcgac
     2521 caactggagg ccctcatcgc cgagggccgc aagatgcccg tcggtggggg tgttctcatc
     2581 gatcgtgcgc aggcgtatga cctcatcgat gagctccgtg ttgcgctaag tgatggcttc
     2641 gccccgcccg tgctggccga gccggcggcg aatcttcccg agctcgaggc gctgcggcgg
     2701 gccgaatccg atgcgcggag ccagctgatt cgcgcggagg aggagatcac ccggttgcga
     2761 cgcgaactcg atgcgcgggt cgccggccag gaggtcaccc gcatagccga ggagcgaagc
     2821 cggacgacgc tcgcggaggc ggaggagcgg agccggtcca tgctcaccga agcggaggac
     2881 gaggcccagc gcatccgcgg cgaagcggaa gccgaggccg ctgagcgcct cgcgcaggcg
     2941 gcgcgggtcg cggcgcagca gctcgaagag gctgatacct acgctttgca gcttctgcag
     3001 cggctcgagg accagattca gctgttcctc gagaacgtgc ggacgggcat tacccaactg
     3061 gagggcaagc ggccatccga ccagcacgcc gaccggcaga ttgcgtcccc attctcggtg
     3121 ggccaagcgg aagatgacga cctggacgat gaggacgagg aaccggaaga agacgatagc
     3181 cgcacccgca atcgacgctt tccgtggttg cgggacgatt gaagtagcta gagacgcagc
     3241 ggcctaatcg gcgcgagaac ggccgccggg ttttcccgcc agatggcggg atcctcgtcc
     3301 gtaaacactt ccacctcgtg cccatccggg tcgaacacgt agatgctctt cgtgatcgta
     3361 tggtcggagg cgccggcgat gggaacaccg gcctcgacga gctgccgata ggcctcgcgc
     3421 aactcctcga ttccatcccc gaccttcacg ccgatgtgat acaggccgac tcggcgtcct
     3481 tcgggtatcg gcgtcgcatc ctcgcccacc tcgatgagca ggagctcgtg atgagttcgg
     3541 ccggccgtga gcgccgctgc cttcccgccg aacacgctcc cgaccacctc gaaccccagc
     3601 aggtctcgat agaaccgcag agagcgctcg aggttccgca cgtagagcac gacgtggccc
     3661 agctctcttg ccttcatggc tctcctcctc cgtccgctgc acgccgccct gttattgcgg
     3721 cggctacccc tcgtcgtagt agtccggatc cggctctcgc cgcgccactc cagcggcgat
     3781 ggccgcccgc gcgaccgcgc gcgacacgcg ccggtgcacc gcgcgatcga gggcgtcggg
     3841 caacagcgcg tcggaaggcg ttactgcgac gagcgcctcg ctagcagcct ggaacacctc
     3901 aagcggcgcc gctcgggccc ggacgtcgag caagccacgg cacactcccg gatacaccat
     3961 caggttgttc acgcgccggc cgctcgcggc aactgccgcg ccggcttcca gcgcctgggc
     4021 cgcgtcgatc tccgggcgcg gattcgagag ggcgaagatg aactggccgt ctcgaatcca
     4081 ctcgggccga atcaggttcg cctgcccggt cgtcgcgatc acgacatcac aacgcgccaa
     4141 tatctcctct acgccttccg ccgccacccc gccgaccgac tccaaccggc gcagcgcgga
     4201 cggatctcga tcggcgccca gcaccgtccg gcccgtgtag gccatgactg atcgcgcgat
     4261 ggcgagtccg gccgctccca gcccgatctg gcccacctgc attgctggca ggcttcgttc
     4321 ggcacgctcg caagcgctca gca
//
LOCUS       DATMBB010000043         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_469003, whole genome shotgun sequence.
ACCESSION   DATMBB010000043 DATMBB010000000
VERSION     DATMBB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469003"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..984
                     /locus_tag="VNL92_00870"
     CDS             211..984
                     /locus_tag="VNL92_00870"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO93298.1"
                     /translation="MRLQGKKAIVTGAAYGNGRGIAVRFAEEGADVLVADVNMERANE
                     TKRMIEDLGRRAVVTQTDVRKRDQVEAMVRTCVAELGGVDIAVANAGVAGLRDETGMP
                     RTFLDLTDEAWDFVIDTNLRGVWLTCQAAGKAMVEQGRGGRLINIASIYSEVGSAGAM
                     AYCTSKGGVRMMTKIMAIEAAPHHITVNAIGPGFIETGMTEPLLRMEEARQQLISRIP
                     AGRLGVPRDIANVALFLASDEAEYMTGTIVFADGGYLTQ"
     gene            1041..>2239
                     /gene="ilvB"
                     /locus_tag="VNL92_00875"
     CDS             1041..>2239
                     /gene="ilvB"
                     /locus_tag="VNL92_00875"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWO93299.1"
                     /translation="MRMSGAEIVLECLRREGVDIFWGYPGGVVLPFYDKLPLYPDIHH
                     VLVRHEQAAAHAADAYARVSGKVGVCLATSGPGATNLVTGITNAMMDSVPMVAITGNV
                     ARSLLGRDGFQEADITGITLPITKHNYLVMNAEDLAYTLREAFHIARTGRPGPVLVDI
                     PKDVFIEEADFHYPEEIAIRGYKPTYEGHPSQLRRAVRLIEEAQRPIVLAGQGVIISE
                     GGQQALQQFVEKAQIPVITTLLGIGALREDHVLSYGFMGMHGMYWANMAAIEADLVIG
                     VGMRFDDRAMGRWADFNPGAKVIHIDIDPAEIGKNIPVTVPIVGDVARVLDALTPLVQ
                     QKTHTEWLKWIDNVRDEHPSLKIPEYPGITMQEVVREIYEATGGDAIIVTGVGQHQMW
                     AAQHYQFR"
BASE COUNT          423 a          689 c          737 g          390 t
ORIGIN      
        1 aaagtctcgt ccctttgtgg ggcggggctt ttttgttcgg aagcttcctg ccgccatgga
       61 gtattgacta tgcataccga caaggggttc tcaccggcct cgggaagggc gcgtatcgcg
      121 agtcgcgtcc cccgcggccg cgtcgtagtc ctgtagcagg gtacttgcgc cggattccgc
      181 cggtgcgcgg gggagagcag gagaagagcg atgcggctgc agggcaagaa ggctatcgtc
      241 acgggggcgg cctacggaaa tggccgtggc atcgccgtgc gcttcgctga agagggcgcc
      301 gatgtcctcg tggccgacgt gaacatggag cgtgccaacg agacgaagcg catgatcgaa
      361 gacctcgggc gccgcgccgt cgtcacgcag accgatgtcc gcaagcgcga ccaggtcgag
      421 gcgatggtgc gaacatgcgt tgccgaactc ggcggagtcg atatcgcggt ggcgaatgct
      481 ggcgtggccg gccttcgtga cgagacgggc atgccgagga cgttcctcga tctcaccgat
      541 gaagcgtggg acttcgttat cgatacgaac ctgcgcggcg tgtggctgac atgccaggca
      601 gcgggcaagg cgatggtcga gcagggccgg ggaggccgtc tcatcaacat cgcctcgatc
      661 tattccgagg ttggctcagc tggtgcgatg gcctactgca cgagcaaggg tggcgtccgc
      721 atgatgacca agatcatggc catcgaggcc gcgccgcacc acatcaccgt caacgccatc
      781 ggaccggggt tcatcgagac cgggatgacc gagccgctgc ttcgcatgga ggaagcgcgg
      841 caacagctca tcagtcgcat cccggcgggc cgcctcggtg tgccgaggga catcgccaac
      901 gtcgctctct tcctcgcctc cgacgaggcc gagtacatga cgggaacaat cgtcttcgcc
      961 gacggcggct accttaccca gtagtccgcc ctcggtgatg tccaggaacg cacagatgat
     1021 taggaggagg cagtaccgcc atgcgaatga gcggagctga aatcgttctc gaatgcttgc
     1081 ggcgcgaagg cgtcgacatc ttctggggat accccggcgg cgtggttctg ccgttttacg
     1141 acaagctgcc gctctatccc gatatccacc acgtcctcgt tcggcacgag caggccgcgg
     1201 cccacgccgc cgacgcgtac gcgcgcgttt ccggcaaggt tggcgtgtgc cttgcgacgt
     1261 ccggtccggg agcaacgaac ctcgttaccg gcattacgaa cgcgatgatg gactcggtgc
     1321 cgatggttgc gattaccggc aacgtggctc ggtcgctctt aggccgcgat ggcttccagg
     1381 aggcggacat cacgggcatc acgttgccca tcaccaagca caactatctg gtgatgaacg
     1441 ccgaagactt ggcctacacg ctccgagagg cgtttcacat cgcccggacg ggacgaccgg
     1501 gacctgtcct cgtcgacatc ccgaaggacg tcttcatcga agaggcggac ttccactacc
     1561 cggaagagat cgccatccgg ggctacaagc cgacgtatga ggggcatccg tcgcagctac
     1621 gacgcgctgt tcggctcatc gaggaggcgc aacgccccat cgttctcgcg ggtcagggcg
     1681 tcatcatctc tgagggaggc cagcaggcgc tccagcaatt cgtcgagaag gcccagatcc
     1741 cggtcatcac gacactgctc ggcatcggcg cccttcgcga ggaccacgtg ttgtcgtatg
     1801 gcttcatggg gatgcacggc atgtactggg ccaatatggc cgccatcgaa gccgatctcg
     1861 tgatcggcgt cggcatgcgc ttcgatgacc gggcgatggg ccgctgggcc gacttcaatc
     1921 ccggcgccaa ggtcatccac atcgatatcg accccgcgga gatcgggaag aacattcccg
     1981 tcacggttcc catcgtcggc gacgtggcga gagtgctgga tgcgctcacg cccctggtgc
     2041 agcagaagac ccacaccgaa tggctgaagt ggatcgacaa cgtgcgcgac gagcacccga
     2101 gcctgaagat acctgagtac cccggcatca ccatgcagga ggtcgtccgc gagatttacg
     2161 aggcgaccgg tggcgacgcg atcatcgtga ccggcgtggg ccagcatcag atgtgggcgg
     2221 cgcagcacta tcagttccg
//
LOCUS       DATMBB010000044         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_349123, whole genome shotgun sequence.
ACCESSION   DATMBB010000044 DATMBB010000000
VERSION     DATMBB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349123"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VNL92_00880"
     CDS             <1..284
                     /locus_tag="VNL92_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93300.1"
                     /translation="ATLIRQMLNRQRRVRQRFGFLYAIYNVWPGEMPKVTFDGFKKQV
                     LKVLREELRKYPSVTMAKPALETLVKERHQKIGGAKVRIAAAGQYLFTR"
     gene            complement(639..1559)
                     /locus_tag="VNL92_00885"
     CDS             complement(639..1559)
                     /locus_tag="VNL92_00885"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HWO93301.1"
                     /translation="MTTVARAFGVARSHLIARVQSSAAAQAPACAAHSGQEPPSAGGV
                     KEEGGGEGGDLTPPSTVADAELLEEVRSVVTSRATYGYRRATAIIRRQRRAQGRPPVN
                     HKRVYRLMREHGLLLRRHFGDRPGRVHDGKVITLKSNLRWCSDCFEVRTWSGERVQVA
                     FVLDCCDREVIAFTATAGFVDGEMIRDLMLAAVERRFGPEVRALPNPIEWLTDNGSVY
                     TAHDTVDQAEALGFVVCTTPPYSPESNGMAESFVKGFKRDYVYLADVRTADELLDQLS
                     GWFDDYNRVRPHRGLKMLSPMEYREINLAS"
     gene            complement(1556..1936)
                     /locus_tag="VNL92_00890"
     CDS             complement(1556..1936)
                     /locus_tag="VNL92_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWO93302.1"
                     /translation="MTQAGKKSEGRPKRRQYTVEERIKLLDEADLPGESISTVARKHG
                     ISPSVMFRWRQLRESGGLTGLKAGEELVPASEMKAARAKIRELQRLLGKKTEEVEILK
                     EGLEYAREKKLLSRSPLPRRGGGE"
     gene            2101..2460
                     /locus_tag="VNL92_00895"
     CDS             2101..2460
                     /locus_tag="VNL92_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93303.1"
                     /translation="MSLEECAKYPRILLGARYLAYLDVMAEEGPTYQDRTTARVLKSA
                     VFQDPDDGLFGLDVLGNSFRHFFLSGMKADNVRKAWEFANRSRDRFKESGDLKLAGYY
                     QRLIAYHPCSVSELLMT"
BASE COUNT          449 a          798 c          812 g          506 t
ORIGIN      
        1 tcgcaacgct gatccgacag atgctgaacc gacaaaggcg ggtgagacag cggtttggct
       61 tcctctacgc catttataac gtgtggcccg gcgagatgcc gaaggtcacc ttcgacgggt
      121 tcaagaagca ggtgctgaag gtcctcaggg aggaactccg taagtatcca agcgtcacga
      181 tggccaaacc tgctcttgag acgctcgtca aagagcggca ccaaaagatc ggtggcgcca
      241 aggtccggat cgccgctgcc ggtcagtacc tcttcacgag atgaccaagg ccctcggcgt
      301 ggaggacacc cagctctgac cgcccatagc ggtggcggtg cagaatgatg attttggccg
      361 gcgttgtcgg gatatgcctg tcccatcgtc gagttccgtt tcgtcgcgat aggcgcgcga
      421 ggtggatcac gcaactatgg gcgttggcgg cctcggcggc gcttctgctg atcaggtgcg
      481 cggtgcgcca gaggtcgacc agcgcggtcg ttaccaggtt gccctgcaag cgacgttcgg
      541 aaacgatgca caatgtggac tattccttct cgcgctgcgc cttggcggat cgatgccgcg
      601 agtcgccgcc tggagttgcc ccttcaaaac cggacagctc agctcgccag gttgatctcc
      661 ctgtactcca tgggtgagag cattttcaag cccctgtggg ggcgaacgcg gttgtagtcg
      721 tcgaaccagc cggacagctg gtcgaggagc tcgtccgcag tgcggacgtc ggccaggtac
      781 acgtagtcgc gcttgaagcc cttcacgaag gactccgcca tcccgttgct ctcgggagag
      841 tacggtggcg tggtgcagac cacgaagccg agggcctccg cctggtcgac ggtgtcgtgg
      901 gccgtgtata ccgagccgtt gtcggtgagc cactcgatgg ggttcggaag cgcgcggacc
      961 tcgggcccga agcggcgttc aacggctgcc agcatgaggt cgcgaatcat ctcgccgtcc
     1021 acgaagccgg cggttgcggt gaaagcgatc acctcgcggt cgcagcagtc gaggacgaag
     1081 gctacctgaa cgcgctcgcc gctccaggtg cgcacctcga agcagtcgga gcaccagcgc
     1141 aggttcgact tgagcgtgat caccttgcca tcgtgcacgc gaccaggcct gtcaccgaaa
     1201 tgccgtcgga gcagcaagcc gtgctcgcgc atcagccggt acacgcgctt gtgattcacg
     1261 ggcggcctgc cctgtgcccg tcgctggcgc cggatgatcg cggtcgcccg tcggtacccg
     1321 taggtcgccc ggctggtaac cacggaccgg acctcctcga gcagctccgc gtctgccacc
     1381 gtgctcggcg gcgtcaagtc gccaccttcc ccgccgccct cctccttgac tccgcctgcg
     1441 ctcggtggct cttggccact atgggctgca caggcggggg cctgtgcagc cgccgacgac
     1501 tgcacccgcg cgatgaggtg ggaacgggcc accccgaacg ccctcgcgac cgtcgtcatt
     1561 cgccgcctcc ccgtcgaggc aacggcgacc gcgatagcag ttttttttcg cgcgcgtact
     1621 ccaggccttc tttcaggatc tcgacctcct cggtcttttt gccaaggagc cgctgcaact
     1681 cccggatttt ggcccgggcc gccttcattt cggacgccgg cacgagctcc tcgcctgcct
     1741 tcaacccggt cagaccgccg gactcacgca gctggcgcca ccggaacatc acgctcggcg
     1801 agatgccgtg cttgcgggcc accgtcgaga tgctctcgcc gggcaggtcc gcctcgtcga
     1861 gcagcttgat ccgctcctcg accgtgtact gccggcgctt cggccggcct tcagatttct
     1921 tccctgcttg cgtcatcgga ctccctcgat tgtggagccg aggtgtccgg tttgatcaag
     1981 gggctaaacc aggcgctaac actccatctc tcgctggagt caccattgcg cggaggaatt
     2041 gacgtcgcgt tcggcttgcg atcagaaggg cacctctata gcgcggcgac agtgagggcc
     2101 gtgagccttg aggaatgcgc caagtaccct cgcatcctgc tcggtgctcg gtacttggcg
     2161 tacttggatg tgatggccga ggaaggccct acgtaccagg accggacgac tgctcgcgtt
     2221 ttgaaatctg ctgtgttcca agacccagac gacggtctct tcggtctcga cgtcttaggc
     2281 aactcgtttc ggcatttctt tctgtcgggg atgaaggccg acaacgtccg gaaggcgtgg
     2341 gaattcgcga accgaagtcg agataggttc aaggagtcgg gagacttgaa gttggccggt
     2401 tactaccagc gtctcattgc gtatcatccg tgttcggtta gcgagttgct gatgacgtga
     2461 gatcgaggcc gttttccgtc ggaaaaggtt gaaatgagcc atggaagctg atctcgttga
     2521 agttggagac aacgacgaga ggaggctgac catggctcaa aagaa
//
LOCUS       DATMBB010000045         2308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_503658, whole genome shotgun sequence.
ACCESSION   DATMBB010000045 DATMBB010000000
VERSION     DATMBB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503658"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VNL92_00900"
     CDS             <1..773
                     /locus_tag="VNL92_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO93304.1"
                     /translation="RGQATRDWTLQLPARLIHVDWREEAIGHNYPAELGIVDDAANAI
                     RELAARIPPAEARPEVAATVAAAKRAIREGAEAQNPALVAIADKLREALPRDGILVAD
                     ATILTYQVLNRTTPIFEPGGLIFPSSYAIGPSLPLAIGAKLGAPGRKVVQVAGDGGYM
                     LNASELATAAQERVPIVSVVINDGGYGILRMLQQLRYEARHIGVDLHTPNFAAIAEAF
                     GIAGVRVGDAMSLGDELALALRADEPRLIEVDMKAMTS"
     gene            770..1813
                     /locus_tag="VNL92_00905"
     CDS             770..1813
                     /locus_tag="VNL92_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWO93305.1"
                     /translation="MTTDTKTVDVALAKLREFGERVYRQVGLSPEDAALVVDVQLEAD
                     LRGVDTHGFQRLPWYVEYIRKGENNPRPNLRVLKETPWSLVVDADNAIGQLACARVMT
                     MTIEKAKSTGLAVGTLRNSNDWGCGAYYPMLAAKEGFVAFCTTTSVPTLAPFGALTRL
                     SGNNPMAFAVPRRDAPPIVLDMALTPVALGKVLRARAEGTTIPESWGFLDREGRPATD
                     PATALSGVIPAIGGYKGTGLSLMMNVLAGILPGGHHTAEVKPGRRGQFFLVISPSLFG
                     ETERFYDEVESMARQVKEAERLPGVEEVYLPGEIEQRTYEQRAVRGVIAYPGSVVAGL
                     VELGRSLGIAFAP"
     gene            1831..>2308
                     /locus_tag="VNL92_00910"
     CDS             1831..>2308
                     /locus_tag="VNL92_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93306.1"
                     /translation="MTIYYSCDSHVVEAPEVFEGLEEKFGERAPRIITEFRGRPGTFL
                     FWPQRGIPLPVGRFGIAGNRLDDPATQERMLKGWEGMNPGVRDPVARLKEQEQDGIVG
                     EVMYPSINMLTFSIEDREVVHAIFRRHNDWIRDYCSYSPERLIGVACLPLPDIEESI"
BASE COUNT          381 a          783 c          801 g          343 t
ORIGIN      
        1 tccgaggcca ggccacacgc gactggacac tccagctccc cgcgcgcctc atccacgtcg
       61 actggcgaga ggaggccatc gggcacaact acccggcgga gctgggcatc gtcgatgacg
      121 cggcgaacgc tatccgcgag ctcgcggccc gcatcccgcc ggccgaggcg cgccccgagg
      181 tggctgccac cgtcgcggcc gcgaagcggg cgatccgcga gggggccgag gcgcagaatc
      241 cggcgctcgt cgccatcgcc gacaagcttc gcgaggcatt gccgcgcgat ggcatcctcg
      301 tggccgacgc aacgatcctc acgtaccagg tcctcaaccg cacgacgccg atcttcgagc
      361 cgggcgggct catcttcccg agctcgtacg ccatcggccc atcgttgccg ctggcgatag
      421 gggcaaagct gggcgccccc gggcgcaagg tcgtccaggt ggccggcgac ggcggctaca
      481 tgctgaacgc atcggagctg gcgaccgccg cgcaggagcg cgtgcccatc gtgtcggtcg
      541 tcatcaacga tggcggctac gggatcctgc ggatgctcca gcagctccgc tacgaagccc
      601 ggcacatcgg tgtcgatctg cacacgccga acttcgcggc catcgccgag gcattcggga
      661 ttgcgggcgt ccgcgtgggg gacgcgatgt cgctcggcga tgagcttgcc ctcgctctgc
      721 gtgccgacga gccccggctc atcgaggtcg acatgaaggc gatgacgtcg tgacgaccga
      781 tacgaagacc gtcgacgtgg ccctcgcgaa gctgcgcgag ttcggcgagc gcgtgtaccg
      841 ccaggtgggc ctatcgccgg aggacgctgc gcttgtcgtc gacgtgcagc tcgaggccga
      901 cctccgcggc gtggatacgc acggcttcca gcgcctgccc tggtacgtgg agtacatccg
      961 caagggcgag aacaacccgc gcccgaacct ccgagtcctc aaggagacgc cctggagcct
     1021 cgtcgtcgac gccgacaacg ccatcggcca gctggcctgc gcgcgcgtca tgaccatgac
     1081 catcgagaag gccaagagca ccggcctcgc cgtgggaacg ctccgcaatt cgaacgactg
     1141 gggctgcggc gcgtactacc cgatgctcgc ggcaaaggaa gggttcgtcg cgttctgcac
     1201 gacgacgagt gtgccgacac tggcgccctt cggcgcgctc acccgtcttt ccgggaacaa
     1261 cccgatggcg ttcgccgtac cccgcaggga tgcgccgccc atcgtgctcg acatggcgct
     1321 gacgcccgtg gcgctgggca aggtcctccg tgcccgggcc gagggcacga cgatccccga
     1381 gtcgtggggg ttcctcgacc gcgagggccg gccagcgacc gacccggcga cggcgctgag
     1441 cggcgtcatc ccggcgatcg gcggctacaa gggaaccggg ctgagtctga tgatgaacgt
     1501 gctcgccggc atcctgccgg ggggccatca cacggcggag gtcaagccag gaaggcgcgg
     1561 ccagttcttc ctcgtcatct ccccatcgct cttcggcgag acggaacggt tctacgacga
     1621 ggtggagagc atggcgcgcc aggtaaagga ggcagagcgc ctgcccggcg tggaggaggt
     1681 gtacttgccc ggagaaatcg agcaacgaac gtacgagcaa cgcgccgtac gcggcgtcat
     1741 cgcgtatccc ggctccgtcg tggccggcct cgtcgagctc ggccgcagcc tcgggatcgc
     1801 gttcgcgccc tgaacgagga gggaagcagc gtgacgatct actattcgtg tgactcgcat
     1861 gtcgtcgagg cgcccgaggt cttcgagggc ctcgaggaga agttcggcga gcgcgcgccc
     1921 cgcatcatca ccgagtttcg cggccggccg gggacgttcc tgttctggcc gcaacgcggc
     1981 atcccgctgc cggtgggccg cttcggcatc gccgggaacc ggctcgacga tccggcgacg
     2041 caagagcgga tgctgaaggg ctgggagggg atgaatcccg gcgtgcgcga tccggtcgcg
     2101 cgattgaagg agcaggagca ggacggcatc gtcggcgagg tgatgtatcc cagcatcaac
     2161 atgctgacgt tctcgattga ggaccgggag gtcgtgcacg ccatcttccg ccgccacaac
     2221 gactggatcc gggactattg cagctattcg ccggagcggc tcatcggcgt cgcgtgcctg
     2281 ccgctgcccg atatcgagga gtcgatcc
//
LOCUS       DATMBB010000046         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_227912, whole genome shotgun sequence.
ACCESSION   DATMBB010000046 DATMBB010000000
VERSION     DATMBB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227912"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2338
                     /gene="dnaE"
                     /locus_tag="VNL92_00915"
     CDS             <1..>2338
                     /gene="dnaE"
                     /locus_tag="VNL92_00915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWO93307.1"
                     /translation="SAAASIILYCVGITDIDPLRHRLVFERFLNLERREMPDIDMDFA
                     DDRRDEILRYVAEKYGHDRVAQIITFGTMGAKAAVRDSGRALGMSYADTDRVARLVPF
                     ALGMTLDRALEESPQLKQAYELDPSIRNLIETARGLEGVARHASTHAAGVVISHDPLV
                     EHVPLQRPARGDDPDALPTTQYAMEQVAQIGLLKMDFLGLSNLTILARAVELIRQTQG
                     IDLDLLELPENDAKTYEMLARGETFGVFQLESGGMRRAIQELRPESIGELAAMVALYR
                     PGPMQHIGTYCRAKHGEEPIEYPHPDLAEILDETYGVIVYQDQVLLIAQKFAGYTLGE
                     ADVMRKAMGKKIPEKMRAERDRFLAGARARGYDEETAANVFDLIEPFAGYAFNKAHAV
                     CYGTIAYQTAYLKANYPVEYLTAVLMQAPNQGTPNRVAAAVAECIRLGIPVLPPDVNR
                     SGETFSIEQLDDGRMGIRFGLADIKNVGQGAAEEIIAARIERGPFASIEDFCRRIGMR
                     QANKRTLESMIKAGALDCLAGRRTLLANIDRISSLAQREQRLRETGQSTMFDLFGDTQ
                     SFPLPALDLQPLETPLTEALAWEKELLGVHVSEHPLTRAAAALAPYVTTMASEIGPDM
                     AGRDAVLAGSVERVRLLSTKQGRPFCAVELEDFSGSVEVTVWPDVYEPTADLWQQGNI
                     LLAYVRVRERDDRVNLAVQRVAQYLPDSEQLALDLDPAEWTVSEARRGVRELPPVRGG
                     ANEGEPAGDTNGSPQPAMTPSNGNVAPSPKPAPQLLRIT"
BASE COUNT          438 a          810 c          730 g          360 t
ORIGIN      
        1 gcagcgcagc ggccagcatc atcctctact gcgtcggcat taccgatatc gaccccttgc
       61 gtcaccgcct cgtcttcgag cgcttcctca acctcgagcg ccgcgaaatg ccggacatcg
      121 acatggactt cgccgatgac cgtcgcgacg agatcctccg ctacgtggcc gagaagtacg
      181 gccacgatcg cgttgcgcag atcatcacct ttggaacgat gggcgcaaag gccgccgttc
      241 gcgacagcgg ccgcgcgctc ggcatgtctt acgccgatac ggatcgggtc gcgcggctcg
      301 taccgttcgc actggggatg acgctcgatc gagccctcga ggagagcccg caactgaagc
      361 aggcgtacga gctcgatccc tcgattcgga acctcatcga gacggcgcga ggattggagg
      421 gcgtggccag gcacgcgagc acccacgcgg ccggcgtcgt catttcccat gatccgctcg
      481 tcgagcacgt gccactgcag cgcccggcgc gcggcgacga cccagacgcg ctgccgacca
      541 cgcagtatgc gatggagcag gtagcccaaa tcgggctcct caagatggac ttcctcggcc
      601 tgtccaacct cacgatcctc gcccgcgcgg tcgagctcat ccgccagacg caggggatcg
      661 acctcgacct gctcgaactg ccggagaacg acgcaaagac gtacgagatg ctggcacgcg
      721 gcgaaacctt cggcgtgttc caactcgaat cgggcggcat gcgacgcgcc attcaagagc
      781 tacgcccgga atcaattggc gagctcgcgg ccatggtggc gctctaccgc cccggtccga
      841 tgcaacacat cggcacctac tgccgcgcga agcacggcga agagccgatc gaatacccgc
      901 accccgacct cgcggagata ctcgacgaga cgtacggggt catcgtgtac caggatcagg
      961 tcctgctcat cgcgcaaaag ttcgcgggct acacgctcgg cgaggccgat gtgatgcgca
     1021 aggccatggg caagaagatc ccggagaaga tgcgagccga gcgcgatcgc tttctcgccg
     1081 gcgctcgcgc ccgcggctac gatgaggaga cggcagcgaa cgtcttcgac ctcatcgaac
     1141 ccttcgccgg atacgccttc aacaaggcgc acgcggtctg ctatggaacc attgcctacc
     1201 agaccgctta cctgaaagcg aactaccctg tcgagtatct caccgccgtc ctcatgcagg
     1261 ccccgaatca aggcactccg aaccgcgtgg ccgccgccgt cgccgaatgc atccgcctcg
     1321 gcatcccggt gctgcctccc gacgtaaatc gatcgggcga aaccttcagc atcgagcagt
     1381 tggacgacgg acggatgggc atccgcttcg gcctcgccga catcaagaac gtgggccagg
     1441 gagcggcgga ggaaatcata gcggcccgga tcgagcgggg tcccttcgcc tccatcgagg
     1501 acttctgccg ccgcatcggc atgcggcagg ccaacaagcg aacgctggag tccatgatca
     1561 aggccggagc gctcgattgc ctggctggcc ggcggacgtt gctcgccaac atcgaccgca
     1621 tctcgtcgct cgcgcaacgg gagcagcgcc tccgcgagac aggccaatcg acgatgttcg
     1681 atctgttcgg ggatacacag tcctttccgc taccggcgct cgacctacag ccgctcgaaa
     1741 cgccccttac cgaggcgctc gcgtgggaga aggagctgct cggcgtccac gtctccgagc
     1801 acccgctgac gcgagccgcg gccgcgctcg caccctacgt gacgacgatg gccagcgaga
     1861 ttggccctga tatggccggg cgcgacgccg tgctcgccgg gagtgtcgag cgcgtgcgcc
     1921 tgctttcgac gaagcagggg cggccgttct gtgccgtcga gctcgaggac ttctccggct
     1981 cggtcgaggt caccgtgtgg cccgacgtgt acgaaccaac ggccgatttg tggcagcagg
     2041 ggaatatctt gctcgcttac gtccgcgttc gcgagcgcga cgaccgcgtc aatctcgccg
     2101 tgcagcgcgt cgcccagtac ctgccggact cggaacaatt ggccctcgac ctcgaccccg
     2161 cggagtggac cgtgtcggag gcgaggcggg gggtgcgcga gcttccaccg gtccgcggcg
     2221 gcgccaacga gggcgaacct gccggcgaca cgaacggaag tcctcaaccc gcgatgacgc
     2281 catcgaacgg gaatgtcgcg ccctcaccca agcccgcgcc acaactgctg cgcatcac
//
LOCUS       DATMBB010000047         9270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_589292, whole genome shotgun sequence.
ACCESSION   DATMBB010000047 DATMBB010000000
VERSION     DATMBB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589292"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VNL92_00920"
     CDS             <1..1157
                     /locus_tag="VNL92_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93308.1"
                     /translation="TPVSHFLAQVNAELPGEAAKIDLTWMDVPTERLQKIKPSERGLL
                     LSQIEIGSYGYVPDTWRRLDDMPRGAWPPDHGGPVGRYSVVEKVDVWAENVRELYEEA
                     ILHRWAPATDVPWHQLEELPWHIELALDQVCTRLSEDALIANQVLNGWFERIAYGFHD
                     AKLYLASVSFETARHTEAFRKRALANGGGLGVQGPAWFHRRVAGSGNFTEFVIHELVQ
                     RTSFKRTMCAALEERAQTGTDRTLFARVGADLDRQLAYSLGHLEYYVQYNQPARQDAR
                     PQVDFWLWNGERFMARDITDDGPMDEALMLVLGDGETVESGKRALLELRRRQIREYIE
                     RLASATIPRAPMPQAKRYDCIAPDLFAIAEPEAWQQMTAAAPPATGARVV"
     gene            complement(1213..1977)
                     /locus_tag="VNL92_00925"
     CDS             complement(1213..1977)
                     /locus_tag="VNL92_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWO93309.1"
                     /translation="MEDAIELGALTALAFLAGAVNAVAGGGSLISFPGLLAAGYDAKP
                     ANVTNTVALWPGYLGGSIGYREELRAQRSRTIVLIAPSVLGALAGSAILLATPGDVFE
                     GIAPFLILFACVVLWFQDRLAALTQTHSSAGASGSRLPATVHIGAFALAVYGAYFGAG
                     LGILMLALLGILIADELQHLNALKGFLSLVTNAVAVIYFALFGPVQWAPALLMAAGAL
                     AGGYFGVGAARRLGRELLRLVVVVYGVAAAILMLVT"
     gene            2173..5928
                     /gene="metH"
                     /locus_tag="VNL92_00930"
     CDS             2173..5928
                     /gene="metH"
                     /locus_tag="VNL92_00930"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWO93310.1"
                     /translation="MSFDRASRLAALPGLLNERILVLDGSWGVFLQSYDLTEADYRGD
                     RFASWPADLKGNHDLLNLTRPDIVRSIHAAYLAAGADITTTNTFTSTAVSQADYGMQA
                     AVYDMNLAGASLARAAADEAEAKEPVRPRYVAGSLGPTNKTASISPDVNDPSARAITF
                     DELADAYEEAARGLLDGGADILLVETVFDTLNAKAAIFAIERLFEKRGERIPIWISGT
                     IVDKSGRTLTGQTSEAFWYSVMHARPAIVGLNCSLGPEDLRPYIEEIASVADCFVSVY
                     PNAGLPNAMGGYDETPESMTRVLRDFAERGWINVVGGCCGTTPQHIAAFAEAVRGLPP
                     RRVPEIEPRLRLSGLEPLVVRREMAGGQLGSTFVNIGERTNVTGSRRFARLILDGKFA
                     EAAEVAAQQVESGAQLLDVNMDEAMLDSAAAMARFLSYIASEPDIAKVPVVVDSSKWS
                     VIEAGLKCVQGKGIVNSISLKEGEEPFLRQAALARRYGAAVIVMAFDEQGQAETTERK
                     VEICHRAYRLLTEHAGFSPQDIIFDPNIFAVATGIEEHNRYAIAYLEAVRAIKRELPH
                     VHVSGGVSNLSFSFRGNERVRRAMHAVFLYHAVQAGLDMAIVNAGQLDVYEDVPEELR
                     ERIEDVLFDRRPDATDRLLEMAERYRGTEERTDAVEAAWRQLDVNARLTYALVHGIAD
                     YVEQDVEEARLQAERALAVIEGPLMDGMRVVGDLFGAGKMFLPQVVKSARVMKRAVAQ
                     LVPHIEAERADRGDGQASGAGKVLLATVKGDVHDIGKNIVGVVLQCNNYEVLDLGVMT
                     PAATILETARRERVDIIGLSGLITPSLDEMVHVAQEMQREGFGLPLLIGGATTSRVHT
                     AVKIAPQYSHPTVWVPDASHAVGVVANLLSDQQRPAFLAQVDAQYEDARALHAGRDRA
                     AALLSLEEARARAPRLVYEPSPPKHPGLGTMADYPLAELRAYIDWTPFFHVWEMKGRY
                     PQILDDPERGAEARRLLADANGLLDRIIEERLLRARAVYGLFPANREGADDIAVYADE
                     GRAEPLAMLHTLRQQSDRGAGRPCLALADYIAPPGTPDYVGAFVVTAGLGLDELAARF
                     RADHDDYGAIMAQALADRLAEAFAERLHQLLRTEYWGYAVDERLSADDLIAEKYRGIR
                     PAPGYPACPDHTEKGTIFRVLAAEETTGVRLTESYAMWPTASVAGLYFAHPDARYFNV
                     GPIGRDQVEDYARRKGVALDEVERWLRPNLAYEPGTAATGAGRPLVR"
     gene            complement(5925..6344)
                     /locus_tag="VNL92_00935"
     CDS             complement(5925..6344)
                     /locus_tag="VNL92_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWO93311.1"
                     /translation="MTTEYTKPLPRPLNPQASGPFWDGAKRHELMMPHCRTCDSLFFY
                     PREACPMCLSPDIDWTRASGRGRLYSYTVIYQPAHPAFAADAPYIYALVQLDEGPRMI
                     SNLVDCAPEAAYVDMPLEAVFDDVTPEVTLVKFRPAS"
     gene            complement(6436..7515)
                     /locus_tag="VNL92_00940"
     CDS             complement(6436..7515)
                     /locus_tag="VNL92_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWO93312.1"
                     /translation="MSLRGKAAIVGIGELPTRRHYPGRSMEGLCAEVARVAIEDAGLA
                     KADIDGLIVEGGGVPPANIAEYIGIRPVFSTGVTMFGASGATSVALAAAAINAGMCTN
                     CLIVMGAARDPELGMGGMRGAFGGIGAEFEMPFGPAAGAGTGYALMYRRHIHEFGTKP
                     EQMAKLAADQRFNALQNPNAVFQGQPITVDDVLSSRYINEPLHILECVMPTAGAGALI
                     LTSEERAKSFPNRPVYVLGAGIEQANAQIWQTQRITVTPARVSARRAFEMAGYSPKDI
                     QFAEFYDCYTILVASTLEDAGFVKKGEIGPFYESTDTTYKGSFPINTDGGQLSAGQPG
                     IAGGLRHVIEAARQVMKNDLCMVNG"
     gene            complement(7572..>9270)
                     /locus_tag="VNL92_00945"
     CDS             complement(7572..>9270)
                     /locus_tag="VNL92_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWO93313.1"
                     /translation="PGEKVPVDGEIVEGRSTLDESMVTGESLPVTKEAGDTVIGATIN
                     QTGAFRFRATKVGADTMLAQIIRLVEQAQASKAPIQRLADQISSYFVPAVIFLAIGTF
                     VIWYNVGPEPEISFALVAAVSVLIIACPCALGLATPLSIMVGTGKGAEAGILIRSAEA
                     LETAHRLNTIVLDKTGTITKGQPSLTDVIATGDVAEDELLRLVASAERSSEHPLGEAI
                     VRGAEERGISLADPSDFESLTGRGIRVRVDGREVLVGNRRLLEERGIDPAALDNEVTR
                     LTGEGKTAMFAAVDGRAAGLVAVADTVKDESARAVAALRSLGLEVVMMTGDNRRTAEA
                     IARQVGIGRVLAEVLPEDKALEVRRLQDEGRLVGMVGDGINDAPALAQADVGIAIGTG
                     TDVAIEAADVTLISGELRGVVTAITLSRATMRNIRQNLVFAFGYNTAGIPIAAGVLYP
                     LISALLNPMIAAGAMAASSLSVVSNANRLRTYAPQPLPELAEPPAVTPKVEVHEREPG
                     GPATVHDPVCGMDIAPEDAAGTSEYEGRTHYFCSPSCKERFDEHPEEYVEQPVSAAM"
BASE COUNT         1563 a         3075 c         3129 g         1503 t
ORIGIN      
        1 gcacgccggt atcgcacttc ttggcacagg tgaatgcgga attgccgggc gaggctgcga
       61 agatcgattt gacgtggatg gatgtgccca cggagcgtct tcagaagatc aagccgagcg
      121 agcggggcct gctcctctcg caaatcgaga tcggaagcta cggctacgtc cccgacacat
      181 ggcggcgcct cgacgatatg ccgcgcggcg cgtggcctcc cgaccatggc gggcccgtcg
      241 ggcgatactc ggtcgtcgaa aaggtggacg tttgggccga gaacgttcgc gagctctacg
      301 aggaggcgat tctgcaccgc tgggcgccgg cgacggacgt gccctggcat cagctggagg
      361 agctcccctg gcacatcgag ctggccctcg accaggtatg tacccgcctc tccgaggacg
      421 ccctcatcgc caatcaggtg ctcaacggct ggttcgagcg catcgcctac ggcttccacg
      481 acgcgaagct ctacttggcg agcgtgtcat tcgagacggc ccgccatacc gaggcgtttc
      541 ggaagcgcgc cctcgccaac ggcggaggct taggtgtaca gggcccggcc tggttccatc
      601 gccgtgttgc gggctccggg aactttaccg aattcgtcat ccacgagctc gtgcagcgaa
      661 cttcgttcaa gcggacgatg tgcgcggcgt tggaggagcg agcccagacc gggacggatc
      721 gtacgctgtt cgcccgggtg ggcgctgacc tcgatcgcca gctcgcttac tccttaggtc
      781 acctcgagta ctacgttcag tacaatcaac cggcgaggca agatgcacga ccgcaggtcg
      841 acttctggct ctggaacggc gagcggttca tggcgagaga catcaccgac gacggaccca
      901 tggacgaggc gcttatgctc gtcctgggtg acggcgaaac cgtcgagtcg ggcaagcggg
      961 ccttgctcga gctgcgccgg cgccagatcc gggagtacat cgagcggctc gcttcggcga
     1021 ctatcccgcg cgcgccgatg ccgcaagcca agcggtacga ctgcatagcg cccgacctgt
     1081 tcgccatcgc cgagccggaa gcgtggcagc agatgacggc cgctgcgccc ccggccacgg
     1141 gagcgcgcgt cgtctgaccg ctgccttgga ttgcagatgg aagttgcggg gaggctctca
     1201 gcctccccgc cgctacgtga ccagcatgag gatggcggcg gccacgccgt agaccacgac
     1261 gacaagcctt agcagttcgc gacccaacct ccgcgccgcg ccaaccccaa agtagcctcc
     1321 ggccagggct ccggcggcca tcagcagcgc gggcgcccac tgcaccggcc cgaacagggc
     1381 gaagtagatg acggccacgg cattcgtcac gagcgataga aagcccttga gtgcattgag
     1441 gtgctgcagc tcgtcggcga tgagtattcc gaggagcgcc agcatcagga tgccgaggcc
     1501 ggcgccgaaa taggctccgt acacggcgag ggcgaacgct ccgatgtgca cggtcgcagg
     1561 cagacgcgaa ccgcttgctc ctgcgctgct gtgcgtctgc gtgagggccg cgagacggtc
     1621 ctgaaaccac aggacgacgc acgcgaagag gatgaggaag ggcgcgatgc cctcgaagac
     1681 gtcgccaggg gtggcgagca ggatggcaga gccggcgagc gcaccgagga cgctgggagc
     1741 gatgagcacg atggtgcgcg agcgctgagc cctcagctcc tcacggtagc cgatcgagcc
     1801 accgaggtat ccgggccaga gcgcgacggt gttggtgacg ttcgccggct tcgcgtcgta
     1861 gcccgcggcc agcagtcccg ggaagctgat gagcgaaccg ccgcccgcga cggcattgac
     1921 cgccccggcg aggaacgcca gggcggtgag cgccccgagc tcgatggcgt cttccatcaa
     1981 gcgccgctgg gggcatatgc gcactcccgc ggcggctgtg ctctgctagg ccgaagggag
     2041 gagatggccg cgagaccggc gttcgcggct gtcgtcttga gccggcacag caagcttcgc
     2101 gccattgggc ctcgtcctca ctcccgcttc cggttaccat agaatctcac acaacaccgc
     2161 cagcggggag acatgagctt cgatcgcgcc tctcgcctag ctgctttgcc gggcctcctg
     2221 aacgagcgca tcctcgtgct cgatggatcg tggggcgtct tcctgcagtc gtacgacctg
     2281 acggaggccg actatcgggg cgaccgtttc gcgagctggc ccgccgacct caagggcaac
     2341 catgacctgc tgaacctgac ccggccggat atcgtgcgat cgatccacgc ggcgtatctc
     2401 gcggcaggcg ccgatatcac cacgacgaac acgttcacgt ccacggcggt ctcccaggcg
     2461 gactacggca tgcaggcggc cgtctacgac atgaaccttg ccggcgcgag cctggcccga
     2521 gccgccgccg acgaggccga ggcgaaggag cctgtgcgcc cacgctacgt cgcaggtagt
     2581 ctcggaccca cgaacaaaac ggcctcgatc tcgcccgatg tcaacgaccc cagcgcgcga
     2641 gctatcacgt tcgacgagct cgccgatgcc tacgaagaag cggcacgcgg cctcctcgat
     2701 ggcggcgctg acatcctcct cgtcgaaacc gtattcgaca cgctcaatgc aaaggccgcc
     2761 atcttcgcga tagagcgcct cttcgaaaag cgaggcgaac ggatccccat atggatctcg
     2821 gggaccatcg tagacaagag cggcaggaca ttgaccggcc agacctccga ggccttctgg
     2881 tactcggtga tgcatgcgag gccggccatc gtcggtctca actgctcgct gggtccggag
     2941 gacctgcgcc cgtacatcga ggagatcgcg tcggtcgcgg actgcttcgt gtctgtctat
     3001 ccgaacgccg ggctcccaaa tgccatgggc ggctacgatg agacaccaga atcgatgacg
     3061 cgggtgttgc gcgacttcgc ggagcgcggg tggatcaacg ttgtcggtgg gtgctgcggg
     3121 acgacaccgc agcacatcgc tgccttcgcg gaggccgtgc gtggcctgcc acctcgccgc
     3181 gtcccggaga tcgagccgcg cctgcgactc tccggcctcg agccactcgt cgttcggcgc
     3241 gagatggcag gcggtcagct gggcagcaca ttcgtgaaca tcggcgagcg aacgaacgtg
     3301 acgggatcgc gtcgcttcgc gcggctgatc ctcgatggca agttcgcgga agccgccgag
     3361 gtggcggcac agcaagtgga gagcggggcg cagctcctcg atgtcaacat ggacgaggcc
     3421 atgctcgatt cggcggccgc catggcgcgc ttcctgagct acattgcatc ggagccggac
     3481 atcgcgaagg ttcccgtcgt cgtcgactca tcgaagtggt cggtcatcga ggctggcctc
     3541 aagtgcgtgc agggcaaggg tatcgtgaac tccatcagcc tcaaggaagg cgaggagccg
     3601 ttccttcgcc aggccgcgct cgcccgacgc tacggagccg ccgtcatcgt catggcgttc
     3661 gacgagcagg gccaagcgga gacgacggag cgcaaggtcg aaatctgcca ccgcgcctac
     3721 cggctcctca ccgagcatgc cggcttctcg ccgcaggaca tcatcttcga tccgaacatc
     3781 ttcgccgtgg ctacgggcat cgaggagcac aatcgctatg ccatcgccta cctcgaagcg
     3841 gtccgagcca tcaagaggga gcttccacac gttcacgtct ccggcggggt gagcaacctg
     3901 tcattctcct tccgcggaaa cgagcgggtg cggcgcgcca tgcacgccgt cttcctgtat
     3961 cacgccgtgc aggcggggct cgacatggcg atcgtcaatg cgggccagct cgatgtgtat
     4021 gaggacgtgc cggaggagct ccgcgagcgc atcgaggacg ttctcttcga ccgcaggccc
     4081 gacgctaccg atcgccttct cgagatggcg gagcgctacc gcggaaccga ggagcgaacc
     4141 gatgcggtgg aggcggcctg gcggcaactg gacgtcaatg cccgactcac gtacgccctc
     4201 gttcacggta tcgcggatta cgtcgagcag gatgtcgagg aggcgcggct gcaggcggag
     4261 cgcgccctcg cggtcatcga gggaccgctc atggatggca tgagggtggt cggcgacctc
     4321 ttcggtgcag ggaagatgtt tctgcctcag gtggtaaaga gcgctcgcgt gatgaagcgg
     4381 gccgtcgcgc agctcgttcc gcacatcgag gcggagcgtg ccgaccgggg cgacggccag
     4441 gccagtggcg caggcaaggt gctgctcgcc accgtgaagg gcgacgtgca cgacatcggc
     4501 aagaacatcg tcggcgtcgt gctgcagtgc aacaactacg aggtgctcga ccttggcgtc
     4561 atgacgcccg ccgcgacgat cctcgaaacg gcgcggcgcg agcgcgtcga catcatcggg
     4621 ctatcgggtc tcatcacgcc atcgctcgac gagatggtgc atgtggccca ggagatgcag
     4681 cgcgagggct tcggcctgcc gctgctcatt ggcggcgcga cgacgtcccg cgtccacacc
     4741 gccgtcaaga tcgccccaca gtacagccat ccgacggtgt gggtgcccga tgcgtcgcac
     4801 gccgtcggtg tcgtcgccaa tctcctcagc gatcaacagc ggccggcgtt ccttgctcaa
     4861 gtcgacgccc agtacgagga cgcgcgcgcg ctccacgcgg gacgcgatcg cgctgcggcg
     4921 ctcctctcgc tggaggaggc gcgggctcgg gcgccccggc tcgtctacga gccatcgccg
     4981 ccaaagcatc cggggctcgg gaccatggcg gactatccgc tcgccgagct gcgcgcgtac
     5041 atcgattgga cgcccttctt ccatgtgtgg gagatgaagg gacgctaccc gcagatcctc
     5101 gacgatccgg agcgtggcgc cgaagcaaga aggctgctgg cggacgcgaa tggcctgctg
     5161 gatcgcatta tcgaggagcg actgctgcgc gcccgcgcgg tctacggcct gtttcccgca
     5221 aaccgcgaag gcgccgacga catcgccgtg tacgcggacg aaggccgggc ggagccgctc
     5281 gcgatgctcc acacgcttcg ccagcagagc gacaggggtg ccggccgtcc atgcctcgcg
     5341 ctcgccgact acatcgctcc acccggaacg cctgactacg tgggcgcgtt cgtcgtgacg
     5401 gcgggcctag gcctcgacga gctcgccgcc cggtttcgcg ccgaccacga cgactacggc
     5461 gccatcatgg cgcaggcgct cgccgatcgt ctcgcggagg cgtttgccga gcggctgcat
     5521 cagctcttgc gaaccgagta ttgggggtat gccgtggacg agcggttgag cgccgacgac
     5581 ctcatcgccg agaagtatcg cggcatccgg cccgctccgg gatatcctgc gtgtcccgac
     5641 cacacggaga aggggacgat cttccgtgtc ctcgccgctg aggagacaac gggtgtccgc
     5701 ctgacggaga gctacgccat gtggcccacg gcgtcggttg ccggcctgta cttcgcgcat
     5761 ccggacgcgc ggtacttcaa tgtcgggccc attggccggg accaggtgga ggattatgcc
     5821 cgccgcaagg gcgttgccct ggacgaagtc gagcgctggc ttcggcccaa cctcgcctac
     5881 gagccgggca cggcggcgac aggggcgggt cgtccgctcg ttcgctagct ggcgggccga
     5941 aacttgacca gcgtcacctc gggcgtgacg tcgtcaaaga ccgcctccag cggcatatcg
     6001 acataggcgg cctcgggcgc gcaatcgacg aggttggaga tcatgcgtgg cccctcgtcg
     6061 agctgaacca aggcatagat gtacggcgcg tccgcagcga aggcggggtg ggccggctgg
     6121 tagatgaccg tgtacgagta gaggcggccc ctgccgcttg cccgcgtcca gtcgatatcc
     6181 ggcgacaggc acatcgggca tgcctcgcgc gggtagaaga acaacgaatc gcacgtacgg
     6241 cagtggggca tcatcaactc gtgccgcttg gcgccgtccc aaaacgggcc gctggcctgc
     6301 ggattcagcg gtcgcggcaa gggcttggtg tactccgttg tcatctgcgc actcccgtcc
     6361 ttcgctcggt ggtggcttct agagggtgtc ctcggacccg aggaccagcg agcacatctc
     6421 gctcgcgata cctcctcaac cattcaccat gcagaggtcg ttcttcatca cctgccgggc
     6481 ggcttcgatg acgtgtcgca ggccgccggc gatgccgggc tgaccggcgg agagttgtcc
     6541 gccatccgtg ttgataggga acgatccctt gtacgtcgtg tcggtcgact cgtagaaggg
     6601 cccgatttcg cccttcttga cgaagcccgc gtcctcgagc gtcgaggcga cgaggatggt
     6661 gtagcagtcg tagaactcgg cgaactggat gtccttcggg ctgtagcccg ccatctcgaa
     6721 ggcgcgccgc gcagagacgc gggcgggcgt gaccgtgatc cgctgtgtct gccagatttg
     6781 ggcgttcgcc tgctcgatgc ccgcgccgag cacgtagacg ggccggttcg ggaacgattt
     6841 cgcccgctcc tccgacgtca ggatgagggc gcccgcgccc gcggtgggca tgacgcactc
     6901 gaggatgtgc aacggctcgt tgatgtagcg cgagctgagc acatcgtcga ccgttatcgg
     6961 ttggccctgg aagacagcgt tcgggttctg gagcgcgttg aagcgctggt cggcggcgag
     7021 cttcgccatc tgctcgggct tcgtcccgaa ctcgtggata tgccggcgat acatgagcgc
     7081 gtagccggtg ccggcgcccg ccgcgggccc gaacggcatc tcgaactccg caccgatgcc
     7141 cccgaacgcg cctcgcatgc cgcccatccc caactccggg tcgcgcgcgg cgcccatgac
     7201 gatgaggcag ttcgtgcaca tgccggcgtt gatggccgcg gccgcgagag cgacggaggt
     7261 cgcgccgctc gcgccgaaca tcgtgacgcc ggtggagaac accggacgga tgccgatgta
     7321 ctcggcgatg ttggccggtg gtacgccacc gccttcgacg atgaggccat cgatatccgc
     7381 cttggcgagg cccgcatcct cgatagcgac gcgcgccacc tcggcgcaca acccctccat
     7441 cgaacggccc ggatagtgcc ggcgggtcgg cagctcgcca atgccgacga tggcagcctt
     7501 tcctctcaac gacatgccac acctcctcct ggacggcgga attgtagcac agcgctgagg
     7561 atagggaagg tttacatcgc cgcggagacc ggctgctcca cgtactcctc ggggtgctca
     7621 tcgaaccgct ccttgcacga gggcgagcag aagtagtggg tgcggccctc gtactcggac
     7681 gtgcctgcgg catcctcggg cgcgatgtcc ataccgcaca cggggtcgtg caccgtcgcc
     7741 gggcctcccg gctcccgctc gtgcacctcc accttcgggg tcacggccgg gggttcggcc
     7801 agctcgggga gcggctgggg cgcgtacgtt cgtagccggt tggcgttcga aaccacggac
     7861 agcgagctcg cggccatcgc cccggccgca atcatcgggt tgagcagcgc gctgatgagc
     7921 ggatagagca cgccggccgc gatcgggatg cccgccgtgt tgtaaccgaa ggcgaacacc
     7981 aagttctgcc ggatgttccg catcgtcgcc cgggagaggg tgatcgccgt tacgactccg
     8041 cgcagctcgc ccgagatcaa cgtaacgtcg gcagcttcga tagcgacgtc ggtgccggtg
     8101 ccgatggcga tgccgacatc ggcctgagcc agcgccggcg catcgttgat cccgtcgccg
     8161 accatgccga cgagcctgcc ttcgtcctgc agccgccgaa cctcgagcgc cttgtcctcg
     8221 ggcaggacct cggcaagtac gcggccgata ccgacctgac gcgcgatggc ctccgcggtc
     8281 cggcggttgt cgcccgtcat catcacgacc tcgagaccga ggctgcggag cgcggcgacg
     8341 gcgcgagcgg actcgtcctt gacggtgtcg gccacggcca cgagtccggc tgcccgacca
     8401 tcgaccgcgg cgaacatagc cgtcttcccc tcgcccgtga gccgcgtgac ctcattgtcg
     8461 agcgccgccg gatcgatgcc gcgctcctcg agcagccggc ggttgccgac gagcacctcg
     8521 cgtccgtcta cgcgcacgcg gatgccgcgc cccgtgaggg attcgaaatc ggacgggtcg
     8581 gcgagcgaga tgccccgctc ctcggcgcca cggacgatgg cctcgcccag tggatgctcg
     8641 gacgaccgtt ctgccgaggc gacgagccgc agcagctcgt cctcggccac gtcgcccgtc
     8701 gcgatgacgt cggtgaggga gggctggccc ttggtgatgg tccccgtctt gtcgagaacg
     8761 atggtgttga gccggtgggc cgtttcgagc gcctcggccg atcgaatgag gatgccggcc
     8821 tcggcgccct tgccggtgcc caccatgatc gagagcggcg ttgccaagcc cagggcgcag
     8881 ggacaggcga tgatcagcac ggagaccgcc gcgaccaggg cgaagctgat ctctggctcc
     8941 ggacccacgt tgtaccagat gacgaacgtg ccgatggcca ggaagatgac ggccggcacg
     9001 aagtagcttg atatctggtc cgcgaggcgc tggatgggtg ccttcgaagc ttgcgcctgc
     9061 tcgacgagcc ggatgatctg cgccagcatc gtatccgcgc ccaccttcgt ggcgcggaac
     9121 cggaacgcgc cggtctgatt gatcgtcgcg ccgatgactg tatcgccggc ttccttcgtg
     9181 accgggagcg attcgccggt gaccatggac tcgtcgagcg tcgaccgtcc ttccacgatc
     9241 tcgccgtcga ccggcacctt ctcgccgggc
//
LOCUS       DATMBB010000048         4265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_28032, whole genome shotgun sequence.
ACCESSION   DATMBB010000048 DATMBB010000000
VERSION     DATMBB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28032"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VNL92_00950"
     CDS             complement(<1..787)
                     /locus_tag="VNL92_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWO93314.1"
                     /translation="MNVRAATSARGISLRLPAGLLLAATVGLATLLHVVLALKSPAPW
                     VVPDEIRHSELAKSLATGLPEIRGRMTFEFGLGYPALLAPTFALFDDVSTAYAVAKAL
                     NAFVLGLTALPAYFLARRFLDEARALVVSLLTVSVPSLLYAGTLMTEVALYPAFVLSL
                     LAIALALDRPSRGTQIGALSAIALASSVKLLAAALVVGYVAAILLFHGLETRRGDAWR
                     ARLRAYGPSWVGLAAIAVALGGATAATGRSPLEALGTYEFVLRH"
     gene            1031..1873
                     /locus_tag="VNL92_00955"
     CDS             1031..1873
                     /locus_tag="VNL92_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93315.1"
                     /translation="MGDTTYSVVRATGTRGLAYYLRVLRVVGGVDFKTRYTDAALGYV
                     WSLVKPLAYFGVLWLVFAHLLRTANQTQDFTLFLLIGLLLFLFFSDAVSAMLPSIVEG
                     GATLRRLAFPPILIPLSASVATGITFCVNVLAFAVFAALEPVSPRVEWLLLVPLLAEL
                     YVLALGVGLLLSALYVRFRDIGQVWELGAQLLFFTSAIFYPVGILPEWAQKIAFLNPL
                     VQVMQDVRHVVLGGASGPYDVSAGDVYSGAGGHLISVAIVLLLFVAALVFFRRESHYF
                     AEKL"
     gene            1870..2607
                     /locus_tag="VNL92_00960"
     CDS             1870..2607
                     /locus_tag="VNL92_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93316.1"
                     /translation="MTAPAIEVVDVSKTFRIPHENRTFLKEYFLQPFRRVTYERNEAL
                     RDVSFTVEQGEFFGVIGPNGSGKSTLLRILAGIYVPDSGHVRVRGLLSPFIELGVGFN
                     FELTARDNIRINGTLAGLTSVELAERFDEILAFAELERFVDQKLKNYSSGMLVRLAYS
                     IAIQIPFDVLLLDEVLAVGDEAFQEKCFATFERFKTEGKTVVFVSHALDLMERFCDRV
                     CLLSSGRLVAIAAAREVVSIYSRRGQG"
     gene            3821..>4265
                     /locus_tag="VNL92_00965"
     CDS             3821..>4265
                     /locus_tag="VNL92_00965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93317.1"
                     /translation="MPGVTIVIVVFNTLTVTQGCLTSVLNARGTRSFEVLVVDNGSDP
                     EVLAWLRTVATAEPAVGYIRLPHNVGFARGVNIAARLARGTHLVLLNSDTVVTDGWLD
                     RLADALDSNPTYGLITPVTNYVGEGIQIDPDAENLPLADVASYARR"
BASE COUNT          695 a         1440 c         1302 g          828 t
ORIGIN      
        1 cgtgccgcag cacgaactcg tacgtgccca gcgcctcgag cgggctgcgg ccggtcgcgg
       61 ccgtcgcgcc gcccagggcg actgcgatcg cagcaaggcc gacccaactc ggcccgtacg
      121 cccgcaggcg cgctcgccac gcgtctcctc gccgggtctc gaggccgtgg aagagcagga
      181 tcgctgccac gtacccgacc acgagcgcgg cggccaggag cttcacgctg gacgcgagcg
      241 cgatcgcgga gagggcaccg atctgcgtcc ctcgggaagg ccgatcgagc gccagggcga
      301 tcgccagcaa cgagagcacg aacgctgggt agagcgcgac ctccgtcatc aacgtgcccg
      361 cgtacagcag cgagggcacg ctcacggtga gaagcgacac caccagcgct cgcgcctcgt
      421 ccagaaagcg ccgggcgagg aagtacgccg ggagcgcggt cagcccgagg acgaaggcgt
      481 tgagagcctt cgccacggcg tacgcggtcg agacatcgtc gaagagcgcg aacgtgggcg
      541 caaggagtgc cggatatccc aacccgaact cgaacgtcat ccggccgcgg atctcgggca
      601 agccggtggc gaggctcttc gccagctcgg agtggcggat ctcgtccgga acgacccacg
      661 gcgcggggct cttcagcgcg agaacgacat ggaggagcgt cgccagcccc accgtcgcag
      721 cgagaagcag tccagccgga aggcgaagcg agatccctcg agcactcgtg gcggcgcgca
      781 cgttcacgtc ggcgttatcg gctgtcgggc tcgagcaagc gagaactgcc tcgcgcgggc
      841 ccaacgccgc tcaggtgtct tcctcccctc cgcgcgatcg gcatcccgcc cagaatgcgg
      901 cttcgtacct cgtgccgaca gtgccgatcg tgccatttcg cctggacgat cggctagacc
      961 ccgcgcacga gcctggccgg gcgcccgcct ggcagcctcg ccggacgctt ctctagcctt
     1021 ctgctcggaa atgggcgaca ccacctattc ggtcgtgcgg gcgacgggca cgcgcggcct
     1081 ggcgtactac ctccgcgtcc tgcgcgtcgt gggcggagtc gacttcaaga cccgctacac
     1141 ggacgcggct ctcgggtacg tctggtccct ggtcaagccg ctcgcctact tcggcgtgct
     1201 ctggctcgtc ttcgcacatc tgctgaggac tgcgaaccag acgcaggact tcacgctctt
     1261 cctgctcatc gggctcctgc tcttcctctt cttctccgat gcggtctcgg ccatgctgcc
     1321 ctccatcgtc gaaggcggcg ccactctccg ccggctcgcc ttccctccca tcctgatccc
     1381 gctctcggcc tcggttgcca cagggatcac gttctgcgtc aacgtgctcg ccttcgccgt
     1441 cttcgcggcc ctcgagcccg tatcgccacg ggtcgagtgg ctgctgctcg ttccgctcct
     1501 cgcagagctg tacgtcctcg cgctcggggt cggcctgctc ctgagcgcgc tctacgtccg
     1561 cttccgcgac atcgggcagg tctgggagct cggcgcgcag ctgctgttct tcacctctgc
     1621 gattttctac cccgtcggca tcctgcccga gtgggcccag aagatcgctt tcctgaaccc
     1681 gctcgtccag gtgatgcagg acgtccggca cgtggtgctc ggcggtgcga gtggccctta
     1741 cgacgtgtcc gcaggggacg tttactcggg ggccggcggc cacctgatat ccgtcgcgat
     1801 cgtcctgctc ctctttgtcg ccgcgctcgt gttcttccgg cgcgagagcc actacttcgc
     1861 ggagaagctg tgacggcccc tgcgatcgag gtcgtggacg tctcgaagac gttccgcatt
     1921 ccgcacgaga accgaacctt cctcaaggag tacttcctcc agcccttccg acgcgtgacc
     1981 tacgagcgga acgaagctct ccgggacgtc agcttcaccg tcgagcaggg cgagttcttc
     2041 ggcgtgatcg gcccgaacgg cagcggcaag agcacgctcc tgcgaattct cgccggcatc
     2101 tacgtccccg attccgggca cgtccgcgtg cgcggcctgc tctcgccctt catcgagctg
     2161 ggagtcggct tcaacttcga gctcacggcg cgggacaaca tccgcatcaa cggcacgcta
     2221 gcggggctca cgagcgtgga gctcgcggag aggttcgatg agatcctggc gtttgccgag
     2281 ctggagcgct tcgtggacca gaagctgaag aactactcct cggggatgct cgtgcgtctc
     2341 gcctactcga tcgcgatcca gatccccttc gatgtgcttc tcctcgacga agtgctggcc
     2401 gtcggcgacg aggcattcca ggagaagtgt ttcgcgacgt tcgagcggtt caagaccgaa
     2461 gggaagaccg tcgtcttcgt gagccacgcc ctcgacctga tggaaagatt ctgcgaccga
     2521 gtgtgccttc tatcgagcgg tcgtcttgtt gcgattgccg cagcccgaga ggtagtgtcc
     2581 atctactctc gtcggggcca aggatgacgt tggatgatga gctgcgctgt cgatgtcgtg
     2641 attcctgtct tcaatcgatg ggatctcacc aagcgatgtc ttgaacactt gagccgacag
     2701 acacttccac accgagtcat cctcgcggac aacggctcga ccgacggtac accgggactt
     2761 gttcgccagt tcttccgaac agtcgaagtc gttgagatca aaagcaactc cggcttctca
     2821 ctcgcctgta atcgagctgc gacgcacggc tccggcgatg tcatcgtgtt actgaacaat
     2881 gacgtgttat gcccgcccaa cttcctcatc gagttagtgg cgccactctt cgctcacacc
     2941 cacctcggat cagtggcggc tctccttctg acgcctgacc ggcgagtaat cgagagtttt
     3001 gggcttacag ccgatccgac gctcgccggt tttcctcgcc tccgcggcca accagtcagc
     3061 gccgctcagg aatctcgacc tgttctcctc ggtccgtcgg gtgcagccgg ggcatatcga
     3121 agaaccgcat gggacgacgt cggcggtctc gacgaaggag tgtttgcata cggagaggat
     3181 gtcgatctcg ccatacaact ccgtgctgct ggctggcagg ccgccgcctc cgcctcagcg
     3241 atcgccgtgc atctaggctc ggcgacggcc ggcccgggat ccgcctggca gcgttatcag
     3301 agcggtttct cacgggggta ctttgtccgt cgctacggga tgctccgaag ccggcaggct
     3361 ccacgcgtac tgatttcgga agcgatcgtg tccctcggcg atgccgtcgc tttctcgcac
     3421 gatctcgccg gcgcgcgtgg acgacttgcg ggatggaagt tcgcacgcgg acgatctccc
     3481 aagtcatggc cacccacgag agccatcgac aggacgctga cgtttggttc gagtctccgc
     3541 cttcgtctcg acgtctttcg taactccgcg gcttcaagcg tccctctaga ttgtgaccgt
     3601 tctcccggtg cacgtaccct ctcctgagtc actccgcaac gcgatgcggt tggtcgctca
     3661 cggcctattc gttcttgtac gggagggtcc gcgagcgttc cttacaggcg ttcatgcatg
     3721 gctccgcaac cggtccccga gcgcctcgcc gccgccgctt gttctggtcg acataagccg
     3781 ccatacggaa atcctctcgc agccgccgct tgcggtatcc gtgcccggcg tcacgatcgt
     3841 gatcgtcgtt ttcaacacac tcacagtgac gcaaggctgc ttgacaagcg tgctgaacgc
     3901 gcgcggaact cgcagtttcg aggttcttgt cgtcgacaat ggctcggatc cagaggttct
     3961 tgcctggctg aggacggtcg cgacggctga acccgcagtg ggctatatac gccttcccca
     4021 caatgtcggt ttcgcacggg gtgtgaacat cgctgcgcga ctcgcgcgcg gcacgcatct
     4081 cgttcttctc aacagcgata cggtagtcac cgatggatgg cttgatcggc ttgctgacgc
     4141 actggacagc aacccgacgt acggactgat cactcccgta accaactacg tgggagaggg
     4201 cattcagatc gatcccgatg ctgagaacct tccacttgct gatgttgcct catatgctag
     4261 gcgta
//
LOCUS       DATMBB010000049         2184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_348692, whole genome shotgun sequence.
ACCESSION   DATMBB010000049 DATMBB010000000
VERSION     DATMBB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348692"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VNL92_00970"
     CDS             <1..548
                     /locus_tag="VNL92_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWO93318.1"
                     /translation="GRIGWVPTNGSFQAFVTGLRVQLGEDVARDWLEGIAANDPEEYP
                     NNIAAVQAVADGEVDVAFVNHYYLFQIREDRGDVPVANHYLSNGDPGALVNVAGVGVL
                     SSSDDQDLALEFVRYLLSTEAQQYFADETFEYPLSLTADVEPIADLPPLAELEPPDLD
                     LSSLSDLEGTLEMLRETGVLP"
     gene            552..2153
                     /locus_tag="VNL92_00975"
     CDS             552..2153
                     /locus_tag="VNL92_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWO93319.1"
                     /translation="MTLAAPLQRIRSEGMSSLALWVPALVVVGAMLLPAIYLIVRASE
                     GGSDVWDIVTRASTRDLLARTAWLALTVTAATIAIAVPAAWLTIRTDLPWRQFWSVAL
                     ALPLVIPSYVGGFVIVAALGPRGILQDWLEPLGVERLPEIYGFWGAWLALTLFTYPYV
                     LLNVQAALRNLDPAIEDASRGLGHGPLSTFFRITLPQLRPATVAGGLLVALYTLSDFG
                     AVSLLRFDSFTRVIYIQYRSSFDRTTAAVLGLMLVALTIAVLSAEAITRGRARYHNAR
                     ATRAAAAPIRLGPWRWPAFAACAFLALVALGMPLAVILYWLIDGLQAGQSISLVWDAA
                     WNAFRASGMAALVAVAASLPIALLAVRRAGPATALLERASYLGHALPGIVVALSLVFF
                     SIRYATPFYQTLVLLVFAYVVLFLPQALGSVRASLLQIPSSLEEAGRSLGRGPLRVLT
                     TITLPLIRPGMWAGAALVFLTAMKELPATLLLSPTGYRTLATVIWSNTNEGLFTQASA
                     AALLLIVIAAAPMALMVILQGRTAR"
BASE COUNT          302 a          772 c          685 g          425 t
ORIGIN      
        1 ggggcagaat cggctgggtc cccacgaacg gttccttcca ggcgttcgtc accggcctca
       61 gggtccaact cggcgaagat gttgcccgcg actggctcga gggcatcgcg gccaacgatc
      121 ccgaggagta tccgaacaac attgccgccg tccaggcggt cgcggacggc gaggtcgacg
      181 tcgccttcgt gaaccactac tacctgttcc agattcggga ggaccgtggc gacgtaccgg
      241 tcgcgaacca ctacctgtcg aacggcgatc ccggtgcgct cgtcaacgtg gcgggcgtcg
      301 gcgtgctcag ctcgagcgac gatcaggacc tcgcgctcga gttcgttcgc tacctcctct
      361 cgaccgaggc gcagcagtac ttcgcggacg agacgttcga gtacccgctc tccctcactg
      421 cagacgtaga gccgatcgcc gatttgccgc cactcgcgga gctcgaacca cccgacttgg
      481 acttgagcag cctctcggac ctggagggaa cgctcgaaat gctgcgggag acgggcgtgc
      541 tgccctgagc catgacactc gctgctccgc tccagcgcat ccggtccgaa gggatgtcct
      601 cgctcgccct ttgggtgccc gcgctggtcg tcgtgggagc gatgctgttg cccgcgatct
      661 acctcatcgt gcgggcaagc gagggcggca gcgacgtctg ggacatcgtc acccgcgcga
      721 gcacccgcga cctcctcgcg cgaacggcgt ggctcgcgct taccgtcact gccgccacga
      781 tcgcgatcgc ggttccggcc gcgtggctga cgattcgtac ggacctcccg tggcggcaat
      841 tctggtcggt agcgcttgca ctgccgctcg tcatccccag ctacgtggga ggcttcgtga
      901 ttgtcgcggc gctcgggcca cgcggcattc tgcaggactg gctggaaccg ctgggcgtgg
      961 agcgcttgcc ggagatctac ggattctggg gcgcctggct cgcgctgacc ctgttcacgt
     1021 acccatacgt gctgctcaac gtgcaagcgg cgctccgcaa cctcgacccg gcgattgagg
     1081 atgcctcccg aggtctcggc cacggccccc tgagcacgtt tttccgcatc acgctgcccc
     1141 agttgcgccc cgcgacggtc gccggcggcc tcctcgtggc gctctacacg ctgagcgact
     1201 tcggggccgt ctccttgctt cggttcgatt cgttcacgcg cgtcatctac atccagtacc
     1261 ggtcgagctt tgaccgcacg acagcggcag tcctcgggct catgttggtg gctctgacaa
     1321 tcgccgtgtt gtcggccgag gcaatcacgc ggggccgggc gcgataccac aacgctcggg
     1381 ccactcgggc tgcagcggct cccatccggc taggcccgtg gcgatggccc gccttcgccg
     1441 cctgtgcctt cctcgccttg gttgcgctcg gcatgccctt ggccgtgatc ctgtactggc
     1501 tcatcgatgg gctccaggcg ggccagtcga tatcgctcgt ttgggatgcc gcctggaacg
     1561 ccttccgcgc ctccggaatg gcggcgctcg tcgcggtcgc cgcgtcgttg cccatcgccc
     1621 tcctcgccgt ccgacgggcc gggccggcga ccgctttgct cgaacgggcc agctacttgg
     1681 gccacgccct tcccggcatc gtcgtggcgc tctcgctggt gttcttctcc atacgctacg
     1741 cgactccctt ctaccagacg ctcgttctcc tcgtcttcgc ttatgtcgtg ctgttcctgc
     1801 cccaggcgct cggttccgta cgcgcgtcgc tcctccagat tccctcgagc ctcgaggagg
     1861 cggggcgcag tctcggccga ggcccgttac gcgtactgac gaccattacg cttcccctca
     1921 tccgcccggg gatgtgggcg ggagccgcgc tcgtcttcct cacggcgatg aaggaactgc
     1981 cggcgacgtt gctcctcagt ccaaccggct accggacact cgcgaccgtg atatggtcaa
     2041 atacgaacga ggggttgttt acgcaggcct ctgcggccgc gcttctgctc atcgtcatcg
     2101 ctgcggcgcc gatggcgctg atggtgatcc tccagggaag gaccgcgcgt tgaaccaagc
     2161 catcacctgc accggcttgc gaaa
//
LOCUS       DATMBB010000050         4323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_310662, whole genome shotgun sequence.
ACCESSION   DATMBB010000050 DATMBB010000000
VERSION     DATMBB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310662"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VNL92_00980"
     CDS             complement(<1..478)
                     /locus_tag="VNL92_00980"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWO93320.1"
                     /translation="MTERRPRKPSQLRERIVEESLRLFANQGYSAVSIRDIAQAAKTT
                     NPMIYYYFGSKDELYRHILQQQIESLKARLVQAAEIEGNPVERLGSFVDAYLSFYFKN
                     DRATFTIRELFGLGSDLYRDTVSEIDAEMRRSLRRILRDGADEGLFREDGHDMAVIS"
     gene            515..1924
                     /locus_tag="VNL92_00985"
     CDS             515..1924
                     /locus_tag="VNL92_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWO93321.1"
                     /translation="MVDGTLLRLSRTCQRSFSPRCYTAEASVHLIVVRWIVTSSKGTT
                     VIDSCQIGRLANGLSVVTTPLPTSQAASVSLFIGAGSRAEEEQYNGLFHYLEHMLFKG
                     TERRPDPIEIGQAIEAVGGTLNAYTTKEMTCYWNRVPFDQLDLAVDVLADMLQHSLLA
                     PEELERERSVVQQEIRRGHDQPGQWAGELLSRAAYGDTPIGRSIAGTVETVQNLGRDD
                     FVTHMQRWYLPSRIVLSVAGNVDHERVMALAERLFAGLPDSAPSSFVPAPEVAPEQRV
                     LVESREIEQCNLALAFRALARQDPDRYPLLVLNSVLGRGMSSRLFREVRERRGLAYSV
                     GSGVSRHLDTGLLSVSAGVSNEKAAEAIQVILQECYRLVDEPVGEEELRKAKDFAIGN
                     FRLGLETAMALGQRAGELLLLDGEIEPVEDVVARIRAVTPEDVQRVARRVIRPETAAL
                     SAVGRVEGEELEATLAAAA"
     gene            1943..2788
                     /locus_tag="VNL92_00990"
     CDS             1943..2788
                     /locus_tag="VNL92_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWO93322.1"
                     /translation="MPEARVNGIRIHYEEHGAGYPLILAHGYSATLAMWERQVEALSA
                     KYRLVLYDMRGHGQSEAPEDFAAYTMEAYAADQAGLLDHLGIEQAFVGGLSMGGMVAL
                     QFAVTYPERVRALLLCDTSAENPPGMAEEAERADQIWAVAQRFIAERGREEFAKRLLA
                     NFQRLPLPNVDPQMQERYVERMANASLHGHFGASKAVRARPSLLPRLPELAMPTLIIV
                     GDRDPLVPAAAAMKQAIPSARTVLFSECGHGTAAWKPDVFNHVVLDFLEAVERGEDVA
                     GLYVA"
     gene            2889..4079
                     /locus_tag="VNL92_00995"
     CDS             2889..4079
                     /locus_tag="VNL92_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWO93323.1"
                     /translation="MLPLEGIRVLDLSRLAPGPFCSMLLGDMGADILMIEAPPSRLPG
                     ASMSNPRPSGDDAQRMAAYNALSRNKKSMILNLQEQKARDIFYELAKSADVVLEGFRP
                     GVVKRLGVDYDTLRGINDRIVYCSLSGYGQDGPYVGLVGHDINYISIGGALGMIGWPG
                     GPPAIPMNVIADFAGGGLHAAYGILLALMARQKTGKGQYVDIAMSDGVLQLISTMATM
                     YFSSGTVPGRGTHMLNGALPNYNVYECRDGKWISLGSLEPHFFANLCRVMGREDLIEH
                     QHNAEKFPEMRQHFAEQFRTKDRAEWFRIMQETDICVGPVYTIDEALSDPHNLHRKMV
                     VEVDAPGVGKVKQVGISVKLSETPGAVRSSAPLQGQHTDEVMSSLGYSSEQIRELREA
                     GIVG"
     gene            complement(4076..>4323)
                     /locus_tag="VNL92_01000"
     CDS             complement(4076..>4323)
                     /locus_tag="VNL92_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93324.1"
                     /translation="ALHVVEEPGYGICNVKVGVLRGVQGEEARSKRVAFSGENRQHDG
                     EDGEAKEVRDHRLRRRGVRRVLEGRLLPILRAGCVPP"
BASE COUNT          750 a         1463 c         1350 g          760 t
ORIGIN      
        1 tcgagatgac ggccatgtcg tgcccgtcct cacggaagag gccttcgtcg gctccgtcac
       61 gcaggatgcg ccgtaaggag cgcctcatct ccgcatcgat ctcgctgacg gtatcgcgat
      121 acaggtcgct acccaggccg aacagctcgc ggatggtgaa tgttgcccga tcgttcttga
      181 agtagaagga gaggtatgca tccacgaagc tgcccaggcg ctccacaggg ttgccctcaa
      241 tctcggccgc ctgcacgagg cgcgccttca gcgactcgat ttgctgctga aggatatggc
      301 gatagagttc gtccttactg ccgaagtagt agtagatcat cgggttcgtc gtcttggctg
      361 cttgtgcgat atcgcggatc gagacggcgc tgtagccctg gttcgcaaag agtcgcaggg
      421 attcctccac gattcgttct cgcaactgcg aaggcttgcg cggacgtcgc tcggtcacgg
      481 tcgctccccc ctctgtctac cgaccgttcg acaaatggtc gacggcactc tactccgcct
      541 ctctcgcacg tgtcaacgat cattcagtcc tcgatgctat actgcagaag catccgttca
      601 cctcatcgtc gtccgctgga ttgttacctc atcgaaaggc acaaccgtga ttgattcctg
      661 ccagattggg agactggcca acggccttag cgtcgtgacg actccccttc cgacttctca
      721 ggctgcgagt gtgagcctgt tcatcggcgc aggatcgcgc gctgaggagg agcagtacaa
      781 cgggctgttc cactacctgg aacatatgct gttcaagggc acggagcgcc gccccgaccc
      841 gatcgagatc gggcaagcca tcgaggcggt cggcgggacc ttgaacgcct acacgacgaa
      901 ggagatgacc tgttactgga accgcgtccc gttcgatcag ctcgacctcg cggttgacgt
      961 gcttgccgac atgcttcagc actcgctcct cgcacccgag gagctggaac gcgagcgctc
     1021 ggtcgtgcag caggagatca ggcggggaca cgaccagcca gggcagtggg cgggcgaact
     1081 cctgtctcgc gctgcgtacg gcgatacgcc tatcggccgc agcatcgcag gcaccgtcga
     1141 gacggtgcag aatcttggcc gagacgactt cgtcacgcac atgcagcggt ggtacttgcc
     1201 atcgcgcatc gtgctcagcg tggccgggaa cgtggaccac gagcgggtca tggcgctggc
     1261 ggagcgcttg ttcgcgggcc tgcccgactc cgctccttcg tcgttcgtgc ccgctccgga
     1321 ggtggctccc gagcagcgcg ttctcgtcga gtcgcgcgag atcgagcaat gcaacctcgc
     1381 cctcgcgttt cgtgccctcg ctcgccagga tcccgaccgc tatcccctgc ttgtcctgaa
     1441 cagcgtgctc ggccgcggca tgagcagccg cctcttccgc gaggtgcgcg agcggcgcgg
     1501 attggcctac tcggtaggct cgggcgtgtc ccgtcacctg gataccggac tcctctcggt
     1561 gagcgccggc gtcagcaacg aaaaggcggc cgaagccatc caggtcatcc tgcaggagtg
     1621 ctaccgtctg gtcgacgaac ctgtgggaga ggaagagctc cgcaaggcga aggacttcgc
     1681 catcggcaat ttccggctcg gcctcgaaac ggcgatggct ctcggacagc gagcgggtga
     1741 gctactcctg ctcgacggcg aaatcgagcc cgtcgaggac gtcgttgcgc gcattcgcgc
     1801 cgtcactccc gaggacgtgc aacgcgtggc gcgtcgcgtc atccgcccgg aaacagcggc
     1861 cctttccgct gtcggccgtg tggagggcga ggagcttgag gcgaccctcg cggccgcggc
     1921 ctagccgccg gcatccaacg tcatgcccga agctcgcgtc aacggcattc gcatccacta
     1981 cgaggagcat ggggcgggct atccgctcat cctcgctcac ggctatagtg cgacgctggc
     2041 gatgtgggag cgtcaggtgg aggccctctc cgccaagtac cgtctcgtcc tgtatgacat
     2101 gcgcgggcac gggcaatccg aagctcccga agacttcgcc gcctacacca tggaggccta
     2161 tgccgccgac caggccgggc tgcttgacca cctaggcatc gagcaggcat tcgtcggcgg
     2221 gctctccatg ggcgggatgg tggccctcca gttcgccgtc acctatcccg agcgtgtgcg
     2281 ggcccttctc ctctgcgaca cctcggccga gaatccgccc gggatggctg aagaggcgga
     2341 gcgtgcggat cagatctggg ccgtggcgca gcggttcatc gccgaacgcg gccgcgagga
     2401 gttcgccaaa cgcctcctcg cgaacttcca gcgcctcccg ctccccaacg tcgacccgca
     2461 gatgcaggag cgctacgtgg agcgcatggc caacgcctcc cttcacggcc acttcggcgc
     2521 atcgaaggct gtccgcgctc ggccctcgct cctgccgcgg ttgccggagc tcgcgatgcc
     2581 cacgctcatc atcgtcggcg accgcgatcc gctcgtgcct gcggccgcgg cgatgaagca
     2641 ggccattccg agcgcgcgca ccgtcctctt cagcgaatgc ggacacggca cggctgcatg
     2701 gaagccggac gtgttcaatc acgtcgtgct ggacttcctc gaggccgtcg agcgcggcga
     2761 ggacgtcgcc ggcctgtatg tagcctgatc cgtcatcgct cgagcgcggc ccgggcgctt
     2821 gacatcctct cgacccgcgc cgatgatgcg ggcgccctca tgcctaccat ccaaagaagg
     2881 aggaccgaat gctgcccctc gaagggatcc gcgtgctcga tctctcccgc ctcgcaccgg
     2941 gaccgttctg ctccatgctc ctcggcgata tgggcgccga catcctgatg atcgaggcgc
     3001 cgcccagccg gctccccggc gccagcatga gcaatccccg cccgagcggc gacgacgccc
     3061 agcgcatggc ggcatacaac gccctctcgc gcaacaagaa gagcatgatc ctcaatctgc
     3121 aggagcagaa ggcgcgcgac atcttctacg agctcgcgaa gagcgccgac gtcgtgctcg
     3181 agggcttccg gcccggcgtc gtgaagcgcc tcggcgtgga ctacgacacc ctgcgcggca
     3241 tcaacgaccg catcgtctac tgctcgctct ccgggtatgg ccaggacggc ccgtacgtcg
     3301 ggttggtggg ccacgacatc aactacatct caataggcgg cgccctgggg atgatcggct
     3361 ggcccggcgg cccgcccgcc atcccgatga acgtcatcgc cgacttcgcc ggcggaggcc
     3421 tgcatgccgc ctacggcatc ttgctcgccc tcatggcccg tcagaagacg ggcaagggtc
     3481 agtacgtcga catcgccatg tccgatggcg tgcttcagct catcagcacg atggcgacga
     3541 tgtatttcag ttccggtacg gtccctgggc gcgggaccca catgctgaac ggcgcgctac
     3601 cgaactacaa cgtctacgaa tgccgggacg gcaagtggat cagcctcggc agcctcgagc
     3661 ctcatttctt cgccaacctg tgccgagtta tgggtcgcga ggacctcatc gagcaccaac
     3721 acaacgccga gaagttcccc gagatgcgac agcacttcgc cgaacagttc cgcacgaagg
     3781 accgtgccga gtggttcagg atcatgcagg agacggacat ctgcgtgggc ccggtttaca
     3841 ccatcgacga ggccttgtcc gacccgcaca acctccatcg gaagatggtc gtcgaggtcg
     3901 acgcgcccgg cgtcgggaaa gtgaagcagg tcggcatatc ggtcaagctc tccgagacgc
     3961 ccggcgccgt tcgcagctcg gcccccctgc agggacagca caccgacgag gtcatgtcgt
     4021 cgctgggcta ctcgagcgag cagatccgcg agctccgcga ggctggcatc gtcggctaag
     4081 gtggcacgca cccggcgcgc aaaatcggga gcaagcggcc ctccaggaca cgacgaacgc
     4141 cacgccgccg caggcgatgg tcgcgaacct ccttggcttc gccgtcctcg ccgtcgtgct
     4201 gacgattctc gccggagaag gcgacacgtt tgctcctggc ttcttcgccc tggactccgc
     4261 gaaggacgcc taccttgaca ttgcagatgc cgtagccggg ttcttcgaca acgtgtaagg
     4321 ccg
//
LOCUS       DATMBB010000051         7307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_310515, whole genome shotgun sequence.
ACCESSION   DATMBB010000051 DATMBB010000000
VERSION     DATMBB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310515"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1019)
                     /locus_tag="VNL92_01005"
     CDS             complement(135..1019)
                     /locus_tag="VNL92_01005"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp family protein"
                     /protein_id="HWO93325.1"
                     /translation="MTYRIGVAGLADGRSSLGLVRAFHRRGHEAFVFEAEEVTIDLER
                     SVVAAQDRRLDELDAVVVKKLGDNTDPRVPLRVNMLHALQSYGVRVFSDPSAIAHVND
                     RYRMTTTLALAGVPIPPTVVTDALEEAADTIERWGAAVLKPVYTSKARGMHLLRAQQP
                     YRLVLRDWEAERTGPYYIQRFVPHAGRDLGIAVLGGRALGAYYRVGRPGDWRTSVQRG
                     GHYERADPAPDIVALAERVASIFGLDFTVVDVVETDAGPLVYEASAFGGFGGLAAACN
                     IDAADAYAEHVVRELKRG"
     gene            complement(1016..2599)
                     /locus_tag="VNL92_01010"
     CDS             complement(1016..2599)
                     /locus_tag="VNL92_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HWO93326.1"
                     /translation="MSSGDERLRGVEQDLAKLGRLAVHQLRRALDSFRTADLAHAEAI
                     IEKDDVADNLNLAIEDVCFNLVAESTMGGDEARFLRSAPKVAANIERIGDAACHIAKH
                     VRIIHIEGITPSHFPMAGLESIALPAVDEAVSAYLKRDLQLAELACSREPQLDAAYVD
                     ALQRLTETLRLHPDDATYLMHVYSVLKYLEKVGDYVLNIGEQALFLISGRRMKFDQYQ
                     QLDRLTGQTPIGSLQFHPYWDGISGALVARLETAEGAFVYKEGSQRKIAPEVSKAEEW
                     DRILSGATPRIIRTATLRDREALLREFVQGQLLSDVLLRDEPLERKRAAVGELVDMVS
                     AVWSGSLSAESPRVDYVRQIRDRLDEVYALHPELQDEADGGHGIALHDLLGQAERVEA
                     GLAPSFSVWLHGDLNLNNVVIDSQTGRLKLIDIHRSHSGDYLEDLAVLLVSFERLAVE
                     GERTRRLLRRLAHHVVLFGRGFARIHSDTTYPQRLRLALARSLITSARVVPDDAHARD
                     LYSRGIGLLDRVRKEPAAS"
     gene            complement(2632..3015)
                     /locus_tag="VNL92_01015"
     CDS             complement(2632..3015)
                     /locus_tag="VNL92_01015"
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HWO93327.1"
                     /translation="MAEKKTKFEYAGRIGADQAAAYLEAMAAGLREQRLRLASGGQSI
                     MLHPAETLRAELEAAFNPEKGKGSLQFELSWRVEEAEAAGDLLISSEEEPEPEGGAED
                     EDEEPMMTEPITNPEGEELVIDRGF"
     gene            complement(3045..3311)
                     /locus_tag="VNL92_01020"
     CDS             complement(3045..3311)
                     /locus_tag="VNL92_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HWO93328.1"
                     /translation="MAEKLTYESRMPAVDAAQRLSELALGLSAGRVRLRAGKREAVCY
                     PTGTVTLRVEVKESSKKGRLELEIEWPVGLQVSGDELELRTDGG"
     gene            complement(3339..4157)
                     /locus_tag="VNL92_01025"
     CDS             complement(3339..4157)
                     /locus_tag="VNL92_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HWO93329.1"
                     /translation="MARIDTHVHGLLSKSFSFSLESFADLVRQGITIGLDGFALTEHF
                     HAPAFWAIHDALRARYPYRNGAYEVGGGFRVLSGAELDVAEGGHIGLIGPLDRLRVLD
                     ERFRPMLSGGQFPTYADLRRASEGLDLVIIANHMLRPSKSLERLTPEEFATVSAFELN
                     GKDHEDGIEERIRGYARRYDKPLVGGSDAHVWAQVGVRSTIIPGEASQASLRRALGEK
                     TTDVATRPNAEAIVQLCSVHKQIAKLAAQPSRSALGLPARRRPLPALAPRLAAT"
     gene            4268..4464
                     /gene="amiF"
                     /locus_tag="VNL92_01030"
                     /pseudo
     CDS             4268..4464
                     /gene="amiF"
                     /locus_tag="VNL92_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291415.1"
                     /note="formamide amidohydrolase; catalyzes the hydrolysis
                     of formamide to formate; frameshifted; incomplete; partial
                     in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
     gene            complement(4712..5161)
                     /locus_tag="VNL92_01035"
     CDS             complement(4712..5161)
                     /locus_tag="VNL92_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWO93330.1"
                     /translation="MSAPIIVERWVPAPPNVVYRYFTSAEWWARWQGTGATLDPRPGG
                     AIHIRMAGSNEAGARGHFVELVPDRRLVFTWGWIDAPFGEVPPGSTLVEVDLIVQGDG
                     TIIRLTHRQLPAGLEPMHEEGWRVYLDRLAARVAGDDPGPDPSLSER"
     gene            complement(5158..5469)
                     /locus_tag="VNL92_01040"
     CDS             complement(5158..5469)
                     /locus_tag="VNL92_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010843447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWO93331.1"
                     /translation="MDALRVVAEPRRREILRLVWDEERTAGDIASQFEVTFGAISQHL
                     AVLRGAGLVRTRRAGNRIYYWADRDRLGPLRAVLEAMWAGVLDHLAETIERDRHEEDA
                     P"
     gene            5561..6001
                     /locus_tag="VNL92_01045"
     CDS             5561..6001
                     /locus_tag="VNL92_01045"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWO93332.1"
                     /translation="MDVTCEIVIHRPREVVAAYAENPSNDCEWIGGIKEARALSHPAV
                     GEGARVERIARFLGRRIAYVLETTEYEPNVLIAMQSVQGPFRMRVTYRFEDTVDGTRF
                     SIRNEGGSGIALRLGAPLMSKALRRNVNGDLRRLKDLLESDGAG"
     gene            6364..6585
                     /locus_tag="VNL92_01050"
     CDS             6364..6585
                     /locus_tag="VNL92_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93333.1"
                     /translation="MKNITLSADERLIEEARAKAREEHTTLNEQFRRWLEDYVGRRER
                     AERALATIRELQTRIRLHGPFARDQLNER"
     gene            6575..7009
                     /locus_tag="VNL92_01055"
     CDS             6575..7009
                     /locus_tag="VNL92_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWO93334.1"
                     /translation="MSGDFIDSNVLLYLFSEQEVEKRDIALALVGEALRGGTAAISFQ
                     VVQEVLNGITRLADPPASQQDARTFLAETLVPLWRVNPSAQLYERALEMRDRYRYSFY
                     DSLIIAAALEAGSTRILSEDFQHGQRIEGLTFNNPFVASRRR"
BASE COUNT         1302 a         2371 c         2390 g         1244 t
ORIGIN      
        1 ttgaccgaag tagcggcgca aacgcgcggc cagcgcttcg tcgtcgacct gcacgcgcac
       61 gtttaccccg gccatgctga gggtgaacgc atgaacggcg cggccccggt ggaaggcgag
      121 gtcaagcgag gcgctcaccc gcgcttcaac tcccgcacga catgctcggc gtaggcgtcg
      181 gcggcgtcga tgttgcaggc agcggcgagg ccgccgaacc ccccgaatgc gctcgcctcg
      241 tacacgagcg ggccggcgtc ggtctcgacg acgtcgacta cggtgaagtc gagtccgaag
      301 atggacgcga cccgctcggc cagggcgacg atatccggcg ccggatccgc gcgttcgtaa
      361 tggccgccgc gctgcaccga agtccgccaa tcgcccggcc ggccgacgcg gtaatatgcg
      421 ccgagagcgc gcccaccgag tacggcgatg ccgaggtcgc gcccagcgtg cggcacgaaa
      481 cgctggatgt agtacggtcc ggtgcgctcg gcttcccagt cccgaagcac gagccggtag
      541 ggctgctgcg cacggagcag gtgcatgccg cgcgccttcg atgtgtacac cggcttcaac
      601 acggccgctc cccaccgctc gatggtgtcg gcggcttcct cgagggcatc agtaacgacg
      661 gtgggcggga tggggacgcc cgcgagagcc aacgttgtcg tcatgcgata gcggtcgttc
      721 acatgtgcga tggcgctcgg atcgctgaag acgcggacgc cgtaggactg cagtgcgtgc
      781 agcatgttca cacgaagcgg gacgcgcggg tcggtgttgt cgccgagctt cttgacgacg
      841 accgcgtcga gctcatcgag gcggcgatcc tgagctgcca ccacactgcg ttcgaggtcg
      901 atcgtgactt cctctgcctc gaatacgaaa gcctcgtgtc cgcggcgatg gaacgcgcgc
      961 accagaccga gcgaagaacg cccatcggcc aagccggcga cgccgatgcg atacgtcacg
     1021 atgccgcagg ctccttacga acgcggtcca ggaggccgat gccccggcta tagaggtcgc
     1081 gcgcgtgcgc gtcatcgggc acaacgcgcg cggaggtgat gagcgagcgc gcgagtgcta
     1141 ggcgaagccg ttgtggatag gtcgtgtcgc tgtgtatccg agcgaagccc cggccgaaga
     1201 gcactacgtg atgagccagc cgccgcagaa gccggcgcgt cctctcgcct tctacggcga
     1261 gccgctcgaa cgagacgagc agcaccgcga ggtcctccag gtagtccccg ctatgcgagc
     1321 ggtggatgtc gatgagcttg agcctccccg tctgactgtc gatcacgacg ttattgagat
     1381 tcaggtcgcc gtggagccac acgctgaagc tcggcgccag tccagcctcg acgcgctccg
     1441 cctggccgag gaggtcgtgg agcgcgatgc cgtgtccacc gtccgcctca tcctgaagtt
     1501 cggggtgcag cgcgtagacc tcgtcgaggc ggtcccgaat ctggcgcacg tagtcgacgc
     1561 ggggagactc ggcgctgagg ctcccggacc agacggcact caccatgtcg accagctccc
     1621 ccacggcggc gcgctttcgc tcgagcggct cgtcgcggag gagcacatcg ctgaggagct
     1681 ggccctgcac gaactcgcgc aacagcgcct cgcggtcgcg tagcgtggcg gtgcgaatga
     1741 tacgtggcgt ggccccggag agaatccggt cccattcctc ggccttgctc acttcgggag
     1801 cgatcttccg ctgcgagccc tccttgtaga cgaaggcgcc ctcggcggtc tccaggcggg
     1861 ccacaagcgc cccgctgatg ccatcccagt agggatggaa ctgtagcgaa ccaatcggcg
     1921 tctggccggt gaggcgatcc agttgctggt attggtcgaa cttcatgcgc cggccgctga
     1981 tgaggaagag ggcctgctcg ccgatgttga gcacgtagtc gcccaccttc tccagatact
     2041 tgagaactga gtacacgtgc atcagatagg tggcgtcgtc cgggtggaga cgcagcgtct
     2101 cggtcaggcg ctgcagggca tcgacgtatg cggcatcgag ttgcggctcc cggctgcagg
     2161 ccaactccgc cagctgcagg tcgcgcttga ggtaggcact gacggcttca tcgacggcgg
     2221 gcagcgcgat actctcgagg ccggccatcg gaaaatgcga tggcgtgatg ccttcgatgt
     2281 ggataatgcg gacgtgcttg gcgatatggc aggccgcatc gccgatgcgc tcgatgttgg
     2341 cggcaacctt gggcgccgac cggagaaacc tcgcctcgtc gcctcccatc gtggattctg
     2401 cgaccaagtt gaagcagacg tcttcgatcg cgaggttcag gttgtcggcg acatcatcct
     2461 tctcgatgat cgcctcggca tgggcaaggt ccgcggtccg aaagctatcg agggcccgcc
     2521 gcagctgatg gacggcgagg cggccgagct tcgctaggtc ctgttcgaca ccgcgtagcc
     2581 gctcatcacc gctagacatc tcgcccccag ggcccccgag cagggtcgcc ctcagaatcc
     2641 gcggtcgatg acgagctcct cgccctccgg attcgtgatc ggttccgtca tcatgggctc
     2701 ttcgtcttcg tcctccgccc caccttccgg ttcgggctcc tcctccgagg aaatgagcag
     2761 gtcgcccgcg gcctccgcct cctcgacccg ccaggatagc tcgaattgca ggctgccctt
     2821 acccttctcc gggttgaagg ccgcttccag ctcggcccgc agcgtctccg ccggatggag
     2881 catgatggac tggccgcccg aggcgagccg caggcgttgc tcgcggagcc cggccgccat
     2941 cgcctccaga taggccgccg cctggtcggc gccgattcgt cccgcgtact cgaacttcgt
     3001 cttcttctct gccaaggtgt gcctccgctc gcgtgagtgt tgggctaccc cccgtccgtc
     3061 cgcaattcca gctcgtcgcc ggagacctgg aggccaacgg gccattcgat ctccagctcg
     3121 agccgtccct ttttcgatga ctccttgacc tcgaccctga gtgtcacggt ccccgtcgga
     3181 tagcaaaccg cttcgcgctt ccccgcccgg aggcgaacgc gtccggcgga caggcccagc
     3241 gccagctcgc tgagccgctg cgcggcatcg accgcgggca ttcgggattc gtacgtgagt
     3301 ttttcggcca tgggacgtac ctcgcgaggt gggaaccgtt atgttgcagc gaggcgggga
     3361 gcgagtgcgg gaagcggacg ccgtctcgcc ggtaggccaa gcgcgcttcg ggatggctgc
     3421 gcggccaatt tcgcgatctg cttgtgcact gagcaaagct gcacgatggc ctcggcgtta
     3481 ggccgcgtcg cgacatcggt cgtcttctcc ccgagagcgc ggcggagcga ggcctgcgac
     3541 gcctcgcccg gaatgatggt cgaccgcacg ccgacctgcg cccagacatg ggcgtcgcta
     3601 cccccgacga gcggcttatc gtagcggcgg gcgtagccgc ggatgcgctc ttcgatgccg
     3661 tcctcgtgat ccttgccgtt gagctcgaaa gcagagaccg tggcgaactc ctccggcgtc
     3721 agccgttcga ggctcttcga ggggcggagc atgtggttgg cgatgatcac caggtcgagg
     3781 ccttcggagg cgcgccggag gtccgcgtag gtcgggaact ggcccccgga gagcattggc
     3841 cggaagcgct cgtcgagcac gcgcagccga tcgagcgggc cgatgaggcc gatgtggccg
     3901 ccttcggcca catcgagctc ggccccgctg agcacgcgaa agcccccgcc cacctcgtac
     3961 gctccattgc gatagggata gcgagcgcga agcgcatcgt gaatggccca gaaggccggg
     4021 gcgtggaagt gctcggtgag ggcgaagccg tcgaggccaa tcgttatgcc ttggcgaacg
     4081 aggtcggcga aagattcgag cgagaacgag aacgacttcg agagaagtcc gtgaacatgg
     4141 gtatcgattc gcgccatcca ttcgctccgt tttgacacat tatcgccgaa cggttgtcat
     4201 tggagtgtta atgggccatg aatggacagt tattgttttc cacattccga tgtgtgtggc
     4261 gccgctcggc agcctgggca tgcccacctg cgaaggccca acggcagtac agtggccgtc
     4321 atcatctgtc accatggcat gtttcccgaa atggggcgcc aatgcgccta caagggcgca
     4381 gagatcatga tccgcacggc cgtgtacacg gtgcccgtta ggcgcgcatg gcggatcacg
     4441 aacgcgaacg cattctgcaa cctgaggtcg gactagaacc cgacacatcg gtggttatat
     4501 gaccaggccc ggagattcct gaatcctgtg cgaggtgttc ccacgattca gcctgggacg
     4561 ctgcaaccca ccgctggcag ggctagatag accgccacgg cccgcagcgg ccatcatcat
     4621 cgtccagagg agccagcgag gctagaagag gctgagctat tcggtccgcg ccagccgcac
     4681 ggcgacctcg atgtgcgcgc ggccacgtcc gtcaacgctc gctcagcgat gggtcggggc
     4741 ccggatcgtc acccgccacc cgggcggcca gccgatcgag gtagacccgc caaccctcct
     4801 cgtgcatagg ctcgaggcca gcgggaagtt ggcggtgcgt caggcggatg atcgtgccgt
     4861 cgccttggac gatgagatcg acctccacga gcgtagagcc gggcggcacc tcgccgaacg
     4921 gtgcgtcgat ccatccccac gtaaagacga ggcgccgatc gggcacaagc tcgacgaagt
     4981 ggccgcgcgc acccgcttcg ttggaacccg ccatccgtat atggatcgcg ccgcccggcc
     5041 gcgggtcaag cgtcgcaccg gtgccctgcc agcgcgccca ccactccgct gaggtgaaat
     5101 agcgatagac gacgttggga ggcgccggaa cccagcgctc gacgataatg ggcgcgctca
     5161 cggtgcatcc tcctcgtggc gatcgcgctc gatcgtttcg gcgagatgat cgaggacgcc
     5221 ggcccacatc gcttcgagga cggcgcgcag cggaccgaga cggtcccggt cggcccaata
     5281 gtagatgcgg ttcccggccc ggcgcgtacg cacaaggccg gccccgcgga gcacggcgag
     5341 atgctgtgag atggcgccga acgtcacctc gaactgcgac gcaatgtcgc cggccgtccg
     5401 ctcctcatcc cagacaagcc ggagtatctc gcgacggcga ggttcggcta cgacgcggag
     5461 agcatccata gcggcgctat tttagcatat gttgaaatag atccctccgt ccgcgtaaac
     5521 tctgtccggc gctctgccag cgaaggaggc gacgaagtcc atggacgtga cctgcgagat
     5581 cgtcatccac cgtccccgcg aggtggtcgc ggcatacgct gaaaatccgt caaatgattg
     5641 cgagtggatc ggcgggatca aggaggcgcg cgcactctcc catccggcgg tgggcgaggg
     5701 tgcccgcgtc gaacggatcg cccgctttct gggccgacgg atagcctacg tcctcgaaac
     5761 cacggaatac gaaccgaacg tcctcatcgc gatgcagtcg gtgcaagggc cgttcaggat
     5821 gcgagtgacg tatcgcttcg aggacaccgt cgatggcaca cggttcagca tccgcaacga
     5881 gggcggctcg ggcatcgccc tccggctggg tgcgccgctt atgtcgaaag cgctgaggcg
     5941 gaacgtgaac ggcgatctgc ggcggctgaa agatctgctt gaatcggatg gcgcgggctg
     6001 acggacgtta gcagcggcag gtccagaacg actagttcgt tgctccgtgc tcctttagga
     6061 acattcccaa gctcatagtc gtgtttgcca ccaaacgtga tccgtgagag ctctcgattc
     6121 cgacagaatc gagcctccta aggttcgata ggaagcctcg actcgatgcc aacggggctt
     6181 gggcacccca tgtcgaggca gcctgccatt atgggcaact gcgtgccgaa gttgagcggg
     6241 ccgcgacgcc catcgcgatg cgaacgtgca catcgcttcg attgccagcg cccaaagcct
     6301 gaccatcgct cactccacgc cacacgatcg atcgtgtaca ctacttccgt gcggaggttt
     6361 gccatgaaga acataacctt gagcgccgat gagcggctca tcgaggaggc gcgagcgaag
     6421 gcgcgtgagg agcatacgac gctcaacgag cagtttcgcc gctggctgga agactacgtc
     6481 gggcgtcgcg aacgcgccga gcgggccctg gcgacaatca gggagctgca gacgcgcatc
     6541 cgtctccacg gtccatttgc tcgggaccag ctgaatgagc ggtgacttca tcgactccaa
     6601 tgttctcctg tatctctttt cggagcagga agtcgagaag cgtgacatag ccctggcgct
     6661 tgtgggcgag gcccttcgtg ggggcacggc ggccatcagc ttccaggttg tgcaggaagt
     6721 cctcaacggc atcacccgcc tcgcggaccc tccggcgagt cagcaagacg cccggacctt
     6781 cctcgctgaa acgctggtcc ctttgtggcg cgtcaatcca agcgctcaac tctatgaacg
     6841 agcgctcgaa atgcgggacc gctaccgata ctcgttctac gactccctga tcatcgccgc
     6901 ggccctagaa gccggctcca ctcgcatact tagcgaagat ttccagcacg ggcagcgcat
     6961 cgagggattg actttcaaca acccgtttgt cgctagccgc cgcaggtagg gataaccaac
     7021 ccgcgcgaga agggaaggcg agcaaggagt cgagcgttga gttctgaaca gaccattatc
     7081 cctggaccgg ggacgccggc aggagcgacg ggcgcagcct aacgctgccc ccgccgcaag
     7141 aggctcgcgc cccgcagatg cacgggagga ccggaatccc gaaccttcgg cggatcccgg
     7201 cggcgccgcg ccttgtcagg ggagcagtcg acgagctacg cgttggtgct ttcgctcggt
     7261 gaaccgctat tcgccgctct gaacctcgac cagctcgtcg gcgacaa
//
LOCUS       DATMBB010000052         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_227590, whole genome shotgun sequence.
ACCESSION   DATMBB010000052 DATMBB010000000
VERSION     DATMBB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227590"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..576
                     /locus_tag="VNL92_01060"
     CDS             43..576
                     /locus_tag="VNL92_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWO93335.1"
                     /translation="MDDNDQQDMNEGAGHRREGYPQPGAHEADRTMYWRLGSMLGVSL
                     VWMYGAMFAMVDRFAHADMNLNFFYMALLMAAPMGVFEVAIMWPMYGARRVNIAVLAV
                     AALITIASFLAIRTQLAVNDERFLESMIPHHSGAILMCEQASITDEQVQDLCDRIVES
                     QRAEISEMEDILDRLDE"
     gene            complement(690..764)
                     /locus_tag="VNL92_01065"
     tRNA            complement(690..764)
                     /locus_tag="VNL92_01065"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Asn,seq:gtt)
     gene            complement(778..851)
                     /locus_tag="VNL92_01070"
     tRNA            complement(778..851)
                     /locus_tag="VNL92_01070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(817..819),aa:Gln,seq:ttg)
     gene            complement(910..998)
                     /locus_tag="VNL92_01075"
     tRNA            complement(910..998)
                     /locus_tag="VNL92_01075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Leu,seq:caa)
     gene            complement(1002..1076)
                     /locus_tag="VNL92_01080"
     tRNA            complement(1002..1076)
                     /locus_tag="VNL92_01080"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1042..1044),aa:Gly,seq:gcc)
     gene            1212..1285
                     /locus_tag="VNL92_01085"
     tRNA            1212..1285
                     /locus_tag="VNL92_01085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1244..1246,aa:Gly,seq:ccc)
     gene            1460..1672
                     /locus_tag="VNL92_01090"
     CDS             1460..1672
                     /locus_tag="VNL92_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93336.1"
                     /translation="MSVRISYNPDLKTLRVEIAPDMHTTRTQSFTHDIVVGFSGDDVR
                     HIEIHGVEPAAVADVKVDVPQAAAAT"
     gene            complement(1698..>2803)
                     /gene="lysS"
                     /locus_tag="VNL92_01095"
     CDS             complement(1698..>2803)
                     /gene="lysS"
                     /locus_tag="VNL92_01095"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWO93337.1"
                     /translation="LEARDLVLLAKALRPPPEKWHGLSDVETRFRQRYLDLMANEDVR
                     RTFRLRSQIVSSIRRYMEREGFLEVETPVLQSSAGGAAARPFFTHYNVLDREFALRIS
                     LELHLKRLLVGGFERVFEIGHIFRNEGLSTRHQPEFTMMESYQAYADYDDVARMLERM
                     VSTVAQDVLGTTRVRFGEHDIDLAPPWRRVTLREAILAATGIDFALYPTFETLAPKAS
                     ELGVAVQEGQGWGKLLDELFTERVQPQLVQPTIVIDYPVELSPLAKRKPGEADLVERF
                     EPFVAGIELGNAYTELNDPIEQRRRLEHQLRLRAAGDEEAELLDEDFLRALEHGMPPA
                     GGLGIGIDRLVMVLTGRPSIREVILFPQLRGPR"
BASE COUNT          522 a          889 c          904 g          488 t
ORIGIN      
        1 cctcaaacaa gcggcaccga aatccgatcc gtcggaggtc ttatggacga caacgaccag
       61 caagacatga acgaaggagc gggccatagg cgagaggggt acccccagcc gggcgcacac
      121 gaggctgatc gaacgatgta ctggcggcta ggcagcatgc tcggggtgtc actggtctgg
      181 atgtatgggg cgatgttcgc aatggtcgat aggttcgcgc acgcggacat gaacctgaac
      241 ttcttctaca tggcgctgtt aatggcggcg ccgatgggcg tgtttgaggt cgcgatcatg
      301 tggcccatgt acggcgcgag gcgcgtgaac atcgctgtct tggccgtagc agcgctgatc
      361 acgatcgcca gcttcctggc gattcgcacg caactcgcag tgaacgacga gcgcttcctt
      421 gagtccatga tcccgcacca ttccggcgca atcctcatgt gcgaacaggc atcgatcacc
      481 gacgagcagg tccaggacct ttgtgaccgt atcgttgaaa gccaacgggc agagatatcc
      541 gagatggagg acatcctcga ccgcctcgac gagtgaccag cgtatcccga ccggtagctt
      601 tgttccagcc gattgggcgc tgcgtgagac caaccgcgga gtgcaaccgg ctgcgtgggc
      661 ctcgccctcc ccgtcggcag ggaggaggct ggctccccga cgaggactcg aacctcgaac
      721 ctagcggtta acagccgccc gctctaccat tgagctatcg gggagtgtgg acgcatctgg
      781 ctggcgagcc aggactcgaa cctgaactaa gggctccaaa ggcccctgtg ctaccattac
      841 accactcgcc aacgtgcggc gcggggatgt cgggtccgtc gtccaggggc cggttggctc
      901 gcctcatgat ggtgccgaag gccgggctcg aaccggcacg agggtcgccc ctcaacagtt
      961 tttgagactg tcgcgtctgc ctgttccgcc acttcggctc gtggagcgga agaagggatt
     1021 cgaaccctcg accccctcct tggcaaggag gtgctctacc actgagctac ttccgccagc
     1081 ctcacccgcg cgacattcga cgaccgcacg ccggccacaa aatcatcgta gggcggggcc
     1141 cagcgcagtg tcaaggaagc gtcgtcgact cgccttcagc cgtcgctggg aatgcggtac
     1201 aataaccgcg cgcgcccaag gtgttgtggc ggcacatctg cttcccaagc agagagcgcg
     1261 ggttcgattc ccgctgggcg ctccactggc atctcgtagc tccatacggg tcctggaagc
     1321 acaggcagga ccatgatggt cgcgttccgt taagtcgagg ccggacgggg ccgaaggacc
     1381 cgccttcggc aggccgaacg gcgatagaat cgagcctgac cggctgctac ggtcatgaca
     1441 acatgaagca agggggcttt tgagcgtgag aatctcctac aaccccgacc tgaagaccct
     1501 tcgagtggaa attgccccgg acatgcacac gacgcgcacc cagagtttca cccacgacat
     1561 cgtcgtcggc ttcagcggtg acgacgtccg ccacatagag atacatgggg tcgaacctgc
     1621 ggccgttgcc gacgtcaagg tggacgtgcc gcaggccgcg gcggccacgt aggtcgtccg
     1681 tacgctccac tgcagcacta acgtgggccg cggagctgcg ggaacaggat gacctcccga
     1741 atgctggggc gcccggtgag taccatcacg agccggtcga tgcctatgcc gaggccgccc
     1801 gcaggcggca tgccgtgctc tagcgctcgc aggaagtcct cgtcgaggag ctccgcctcc
     1861 tcatcgccgg ccgccctcag gcggagctgg tgctcgaggc gacggcgttg ctcgatgggg
     1921 tcgttcagct ccgtgtaggc attgcctagc tcgatgccgg ccacgaatgg ctcgaagcgc
     1981 tcgacgaggt cggcctcgcc cggcttccgc ttcgccaggg gcgaaagctc gactgggtaa
     2041 tctatgacga tggttggctg gacgagttgc ggctgcacac gctccgtgaa cagttcgtcg
     2101 aggagcttac cccacccctg cccctcctgg acggcgacgc cgagctcgga cgccttgggc
     2161 gccagcgtct cgaaggtggg atagagcgcg aagtcgattc ccgtcgcggc caggatcgcc
     2221 tcgcgcagcg tgacacgccg ccacggtggc gccaggtcga tatcgtgctc gccgaatcgc
     2281 acgcgcgtcg ttcccagcac atcttgagcc acggtggaaa ccatccgttc cagcatgcgc
     2341 gcgacatcat cgtagtccgc gtaggcctgg tagctctcca tcatcgtgaa ctcgggctga
     2401 tggcgcgtcg acaggccctc gttccgaaag atgtggccga tctcgaagac acgctcaaag
     2461 ccgccgacga gcaatcgctt gaggtgcagc tccagcgaga tgcgcagcgc aaactcgcgg
     2521 tcgaggacgt tgtagtgcgt gaagaacggt cgagcggccg ctccgccggc gctcgattgc
     2581 agcaccgggg tctcgacttc gaggaagcct tcccgctcca tgtaccggcg tatcgagctg
     2641 acgatctggc ttcgtaggcg gaacgttcgg cgcacgtcct cgttcgccat gaggtcgagg
     2701 tagcgttgac ggaagcgcgt ctccacatcg gacaggccgt gccacttctc gggcggcggg
     2761 cgcagcgcct tggcgagcag cacgaggtcg cgtgcttcga gcg
//
LOCUS       DATMBB010000053         5235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_310215, whole genome shotgun sequence.
ACCESSION   DATMBB010000053 DATMBB010000000
VERSION     DATMBB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310215"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VNL92_01100"
     CDS             <1..659
                     /locus_tag="VNL92_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWO93338.1"
                     /translation="ALGDWAPGEPSPWHPAMPERDHGPHVGLIGRRTEQAHIALAVPG
                     VSLHHPDREAVGMLSTALGEGMSSRLFLEVRERRGLAYDVHAYVSHYLDAGTLNVYAG
                     VDPKRLDEAIGAIVGELNRVSDDGVTEDELRKVRELLKGRLLLRMEDTRAVSAWLGGQ
                     ELLLGRIRSIDEAVAEIEAVTARDVQRVARDLLRPDAFHLAVVGPYRSEERIARTLRN
                     "
     gene            complement(683..1399)
                     /locus_tag="VNL92_01105"
     CDS             complement(683..1399)
                     /locus_tag="VNL92_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWO93339.1"
                     /translation="MDITSPVRAIIYDCGNTLLYAHPSIEEICRMLELEHGHRLDAKA
                     FQNALPDWGAMYYRSIGGMYMSDVDVRAGWAEHYGAAVAQVLAVAPAEAVEVGGLIYD
                     WFAHPARWTPYPDVVPTLAEAKRRGYIQGVVSDWGSDLHGILVGHDLTKYLDFVVASA
                     VVRVAKPHSDLFAQALQRAGVAADEALYVGDSYVADVIGARTAGLRAVLIDREGKAPP
                     VDCDVVRSLEEVLALAGACA"
     gene            complement(1440..3890)
                     /gene="lon"
                     /locus_tag="VNL92_01110"
     CDS             complement(1440..3890)
                     /gene="lon"
                     /locus_tag="VNL92_01110"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWO93340.1"
                     /translation="MPPEPEVPESPRNDAESQPSSEQAEEQAPSIPEVLPAIASGPNV
                     LFPGLVVPMLASEESLIKAVDEAVAAETKTLGIFAQQPTEDGEHTGAVYDVGTAANIV
                     RMVRLPNGHIQALLQGTRRIRRLETLQEQPSLRLRVEPIEDVVEPGTELEALRRTALE
                     IFGRIVQLSESLANELTVAAQQVESPSALCDFMAANVGISPDQRQRVLEAANVNERLR
                     ILIEGMQQELNVLEVGSQIRSQVAGELDKTQREFILRQQLRAIQRELGEADAAGQGLE
                     DLTARLDAANLPEAARNEADRELRRLQTMSPQSPEWQVARSYLEWLADLPWSKSTEDN
                     LDIERARTILDEDHYGLDKVKERILEFLAVRKLRGEAPGPILCFVGPPGVGKTSLGQS
                     IARAMGRKFTRMSLGGIRDEAEIRGHRRTYIGSMPGRIIQELRRAGVNNPLIMLDEID
                     KLGMDFRGDPASALLEVLDPAQNHTFTDHYLDVPFDLSHVLFITTANLLDPIPAPLRD
                     RMEVIELAGYTEAEKLQIALRYLVPRQIEANGLSQQHIQFTEDGVRRIIEEYTREAGV
                     RNLEREIGRVCRSVARRVAEGEGELTIVTADTVRELLGPAPIRAARLTVEPDSVGVAI
                     GLAATMFGGDVLAVEASSVPGSGKFTLTGQLGDVMQESARAALTYARMRSGPLGIPDD
                     YFEKHDIHVHVPAGAIPKDGPSAGVTMATALISAVTRRPVRRDVAMTGEITLRGRVMP
                     VGGIKEKVLAAHREGAHTVVLPKDNLVDLEEVPEDVRRELTVVPAEIVDDVLNVALHP
                     ETKPEEHRLAVVAGEMGR"
     gene            complement(3890..4336)
                     /locus_tag="VNL92_01115"
     CDS             complement(3890..4336)
                     /locus_tag="VNL92_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWO93341.1"
                     /translation="MVKQQRSMSEFERFHHQMERMWSQLVGDMVVPPSYCTNVFDPPV
                     DVYEENGRVVVVMEIAGIRGQEIGIEVDEQRLTVRGVRPGDAQGAKRSYSQLEICRGE
                     FRRNITLPVPVDVGSIEVAYEDGFLRLSLAKASVPGKILVRRRTTS"
     gene            complement(4330..4830)
                     /locus_tag="VNL92_01120"
     CDS             complement(4330..4830)
                     /locus_tag="VNL92_01120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93342.1"
                     /translation="MESPVVTVREARLGDLEALLRWYAQLNLPGEPPATPERPTEAHQ
                     RALATILARDDVWLLIAERAGVGVGTVQLTIVPNLTHGGRPWANVENVVVDEHARGLG
                     VGSALLDEAVGRAQAAGCYKISLTSRVEREAAHRFYEARGFQARHRGYSRYFPDVERS
                     PREQGW"
     gene            complement(4843..>5235)
                     /locus_tag="VNL92_01125"
     CDS             complement(4843..>5235)
                     /locus_tag="VNL92_01125"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HWO93343.1"
                     /translation="LVVTEQGFGKRTLVAQYPVHNRGGSGVLTYRLSDRTGNVAAVRL
                     VNGNQELMIISASGIVLRTTVQSIAQQGRSTQGVTVMRVGPGDRVASIEAIDNGEASS
                     SPPSIGSENGNGRGARRRRTRSSDNGAS"
BASE COUNT          871 a         1757 c         1684 g          923 t
ORIGIN      
        1 gcgcgcttgg agactgggcg cccggcgagc cgagcccctg gcatcctgcc atgccggagc
       61 gcgaccacgg gccacatgtc gggctcatcg ggcggcgaac ggagcaagcc cacatcgccc
      121 tcgccgttcc cggcgtctcg cttcaccacc ccgaccgcga agcggtaggg atgctctcca
      181 cggcactggg cgagggcatg tcatcgcggc tgtttctcga ggtccgcgag cggcgcggcc
      241 tggcctacga cgtgcatgcc tacgtcagcc actacctcga cgccggtacg ttgaacgtct
      301 acgcaggggt cgacccaaag cgcctcgacg aggcgatcgg cgccatcgtc ggcgagctca
      361 acagagtcag cgacgacggc gtcaccgagg acgagctgcg caaggtgcgg gagttgctaa
      421 agggacgcct cctgctccgc atggaggaca cccgggcggt atcggcctgg cttggcggcc
      481 aggaactgct actgggacgc atccgctcca tcgacgaggc agtcgccgag atcgaggcgg
      541 tcacggcgag ggacgtgcag cgggtagccc gcgaccttct gcggccggat gccttccatc
      601 tcgcagtcgt cggtccctat cggagcgagg aacgaatcgc gcgaacacta cgaaactgat
      661 tgctggcacg ccctcgggcg ggctaggcgc acgctcccgc cagcgccagg acctcttcca
      721 acgatcgaac gacgtcgcaa tcgaccggtg gcgccttacc ctcccgatcg atcaataccg
      781 cccgtaggcc ggccgtgcgc gctccgatga cgtcggccac gtacgaatcc ccgacgtaca
      841 gcgcctcgtc ggcggcgacc cctgcgcgct ggagcgcctg cgcgaagaga tcggaatggg
      901 gctttgccac ccgcacgacg gcagaggcca cgacgaagtc gagatatttc gtgaggtcat
      961 ggcccacaag gatgccgtgc aggtcgctcc cccaatcgga cacgacgccc tgaatgtaac
     1021 cgcgccgctt cgcctcggcc agtgtcggca ccacatccgg gtacggtgtc cagcgggcag
     1081 gatgcgcgaa ccaatcgtag atgagcccgc cgacctcgac agcttccgcg ggggccacgg
     1141 ccagaacctg ggccacggct gcgccgtaat gctcggccca acccgcccgc acgtcgacgt
     1201 cgctcatgta catgccaccg atggagcgat agtacatggc cccccaatcg ggaagcgcgt
     1261 tctggaaggc tttcgcatcg aggcggtgcc cgtgctctag ctcgagcatc cggcagatct
     1321 cctcgatgct cggatgggcg tagagcagcg tgttcccgca gtcgtagatg atggcccgaa
     1381 cgggcgatgt tatgtccata tctctactgt actcttcgta gaggcgcgcg gaggagtcct
     1441 cagcgtccca tctcccctgc gaccacggcg agacggtgct cctcgggctt cgtctccgga
     1501 tgcaaggcga cgttcaacac atcgtcgacg atctcggcgg gaacgacggt cagctcccgg
     1561 cgcacgtctt ccggcacctc ttccaggtcc acgagattgt ccttcggaag gacgacggtg
     1621 tgggcgccct cccggtgcgc cgccagcacc ttctccttga tcccgccgac cggcatcacg
     1681 cgcccccgca aggtgatctc gccggtcatc gcgacgtcgc ggcgcacggg gcggcgtgtc
     1741 acggcggaga tcagcgctgt agccatcgtc acgcccgcgg atggcccgtc cttgggtatg
     1801 gccccagcgg gcacgtgcac gtggatatcg tgcttctcga agtagtcgtc ggggatgccg
     1861 agcggcccgg agcgcatgcg tgcgtaggtg agggccgcgc gcgccgattc ctgcatcacg
     1921 tcccccagtt gtccggtaag cgtaaacttg cccgaaccgg gaaccgagga tgcttcgaca
     1981 gccaggacat cgccaccgaa catagttgca gcgagcccga tggcaacgcc cacactatcc
     2041 ggctccacgg ttaggcgcgc ggctcgaatg ggtgcaggcc ccaggagctc gcgcaccgtg
     2101 tcggccgtca cgatcgtcag ctccccttcg ccttccgcta cccttcgcgc caccgaacga
     2161 cacactcgac cgatctcgcg ctcgaggttc cgcacgccgg cctcgcgcgt gtactcctcg
     2221 atgatgcgcc gcactccgtc ctctgtgaac tggatgtgct gctggctcag gccgttggcc
     2281 tcgatctggc ggggtacgag gtatcgcagc gcgatctgca gcttttcggc ctccgtgtag
     2341 ccggccaatt cgatgacctc catgcggtcg cgcagcgggg cgggaatcgg atcgagcagg
     2401 tttgcggtcg tgatgaacag cacgtgcgac aggtcgaacg gcacgtcgag gtagtgatcg
     2461 gtgaacgtgt ggttctgggc ggggtccagg acctcgagga gggccgaagc cggatcgccg
     2521 cgaaagtcca tgcccagctt gtcgatctcg tcgagcatga tgagcgggtt gttgacaccg
     2581 gcgcgtcgca attcctgaat gatgcgcccc ggcatcgagc cgatgtacgt gcgtcggtgg
     2641 ccgcggatct cggcctcgtc gcggatgccg ccgagcgaca tacgcgtgaa cttgcgaccc
     2701 atggcgcggg cgatggattg cccgagcgat gtcttgccga caccgggcgg gcccacgaag
     2761 cagagaatcg gccccggggc ctcgccgcgg agcttgcgta cggcgaggaa ctcaaggatc
     2821 cgctccttca ccttgtcgag gccgtaatgg tcctcgtcga ggatcgtgcg ggcccgctcg
     2881 atatcgaggt tgtcttcggt gctcttcgac cacggtagat cggcgagcca ttccagatag
     2941 ctgcgcgcca cctgccactc gggggactgt ggcgacatgg tttgcagccg cctcaactcg
     3001 cggtcggcct cgttccgcgc cgcctcggga agattggcgg cgtcaaggcg tgcggtgagg
     3061 tcctcgagcc cctgaccagc agcatcggcc tcgccaagct cgcgctggat ggcgcgaagc
     3121 tgctggcgga ggatgaattc acgttgcgtc ttgtcgagct cgcccgccac ctgcgacctg
     3181 atctgactcc ccacctccaa cacattcagc tcttgctgca tcccctcgat gaggatgcgc
     3241 agccgctcgt tcacgttcgc tgcttccagg acgcgttgac gttggtcggg cgagatgccg
     3301 acgttcgccg ccatgaagtc gcagagggca gagggagatt cgacctgctg ggcggcgacg
     3361 gtcagctcgt tcgcgaggga ctcgctgagt tggacgatgc gcccaaatat ctcgagcgca
     3421 gtgcgcctca gcgcctcgag ttcggtgccg ggctcgacaa catcctcgat cggctccacg
     3481 cgcaggcgca aggaaggctg ctcctgcagc gtctcaagtc ggcgaatgcg gcgagtaccc
     3541 tgcagcaggg cctggatgtg gccgttcggg agtcgcacca tgcgaacgat gtttgcggct
     3601 gttcccacat cgtagacggc gcctgtgtgc tccccgtcct ccgtgggctg ctgggcgaag
     3661 atgccgagcg tctttgtctc ggctgcgact gcttcatcga cggccttgat taagctctct
     3721 tcggaggcga gcatgggcac cacgagaccc gggaagagca cgttggggcc cgacgcgatg
     3781 gcgggtagaa cctcgggaat ggagggtgct tgctcctcgg cttgttctga agacggctgg
     3841 ctctctgcgt cgtttcgcgg gctttcaggg acctcgggct cgggtggcat caggacgtcg
     3901 ttcggcggcg caccagaatc ttgcccggta cggacgcttt cgccagggag agccggagga
     3961 atccgtcctc gtacgcgacc tcgatactac ccacgtcgac aggcacgggc agggtgatgt
     4021 ttcggcggaa ctccccgcga catatttcga gctgcgaata gctacgcttc gcaccctgag
     4081 cgtcgcccgg acgaactcct ctcacggtca agcgctgttc gtcgacctca atgccgatct
     4141 cctgaccgcg aatcccggcg atctccatga ccaccacgac cctcccgttc tcctcgtaga
     4201 cgtcgacggg aggatcgaag acgttggtgc agtagctcgg cggtacgacc atatccccca
     4261 ccagctgcga ccacatcctt tccatctggt gatggaaccg ctcgaactcg gacatgcttc
     4321 gttgctgctt caccatcctt gctccctcgg agaacgctcg acgtcaggaa agtagcggct
     4381 gtagccgcgg tgccgagcct ggaacccgcg cgcttcatag aagcgatgcg cagcctcgcg
     4441 ctcgacgcgg ctcgtaaggc ttatcttata acacccggcg gcctgagcgc ggccaacggc
     4501 ttcgtccagg agggcgctac cgacgcccaa gcctcgcgca tgctcgtcca ccaccacatt
     4561 ctccacgttg gcccagggcc ggccaccatg cgtcagattc ggaacgatgg tgagctgcac
     4621 ggttccgacg ccgaccccag cgcgctccgc gatgaggagc cacacgtcat cgcgcgccag
     4681 aatcgtcgcc agggcgcgtt ggtgcgcctc cgttgggcgc tctggggtcg ctggaggctc
     4741 gccggggaga ttgagctgcg cgtaccacct gaggagggct tccagatcgc ccaaccgcgc
     4801 ttcacggacg gtgacgacgg gggactccat gaggagccgc ctttagcttg ctccgttatc
     4861 cgacgagcgg gttcgccggc ggcgggcgcc gcgaccgttc ccgttctcac tcccgataga
     4921 aggcggcgac gagctcgctt cgccgttgtc gattgcctcg atggaggcga cacgatcgcc
     4981 cggtccgacc cgcatcacgg tcacaccctg ggtcgagcgc ccctgctggg caatgctctg
     5041 cacggtcgtc cgcaggacga tgccgcttgc ggagatgatc atcaactcct ggttgccgtt
     5101 caccaagcga acggccgcaa cgttgccggt ccggtcgctc aggcggtacg tgagcacccc
     5161 cgagccaccc ctgttgtgaa caggatattg agccaccaac gttcgcttcc cgaacccttg
     5221 ctcggtgaca acgag
//
LOCUS       DATMBB010000054         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_513735, whole genome shotgun sequence.
ACCESSION   DATMBB010000054 DATMBB010000000
VERSION     DATMBB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513735"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1060
                     /gene="cmk"
                     /locus_tag="VNL92_01130"
     CDS             341..1060
                     /gene="cmk"
                     /locus_tag="VNL92_01130"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWO93344.1"
                     /translation="MSAESAGIVTLSKPRTVAIDGPVASGKSSVGRLLAQRWGHVFLD
                     TGMMYRAMTWWALRHGIAPDDAAALARLASEARITIEPTAEVPRVMIDGEDATPHMRD
                     REVEANVSMVSAVPGVRSALVREQRRIAEQQPVVMAGRDIGTVVLPDADLKVYLDASV
                     ETRARRRHGELDRGSRTLEEVREELTARDRLDSERSVSPLRPADDAIILDVDHLGLAE
                     VVERIAAFAGCEGDGTAEATS"
     gene            1057..1800
                     /locus_tag="VNL92_01135"
     CDS             1057..1800
                     /locus_tag="VNL92_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWO93345.1"
                     /translation="MTAARPGLVVRVCAGIVNTVVRGLAFAFTRWRVEGREHVPREGS
                     VILVANHITNLDPPILATATHRHVTYMAKQELFDLPVIGFVFRLYGAFSVRRFEADLA
                     ALRQAQNVLKGGQVLGMFPEGTRSRGSGLGAPYPGAALIALRSNALIVPAAITGTERL
                     MGLGLILFPFTRPRITVRFGEPFRLPQTARPRPDDIKRGSMQIMRSIAELLPEPYLGP
                     HGRALLAGEDIPLAAGPPAHYLEKTAGQR"
     gene            complement(1828..>2624)
                     /locus_tag="VNL92_01140"
     CDS             complement(1828..>2624)
                     /locus_tag="VNL92_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWO93346.1"
                     /translation="RTARELGRRLLHEGLRRGLPVSGPRPLVPISMSVGVATASGAEH
                     ADEVVRRASEAQWLAATNGGQVVVAYGEQLAKQTATVNGLLEALSSKMPDAREQARQV
                     AHYALLLAKAVELPKEQHLALKIAALLHDIGNLVVPDALLHKASELTAEELAVVREHV
                     HVSRRILEASPPLAPAATAAFAHHEHWDGSGYPCGLKGDEIPVSARILSVADAYVTIR
                     AGRASGNVPSHADAIRELRAAAGTRLDSRLVDAFVQALDTESALVA"
BASE COUNT          415 a          894 c          888 g          427 t
ORIGIN      
        1 agatcatccg cgtgcgggcg acacgatcgc tgagaagtag tgcctgaacg ccggccacgt
       61 tgcatgcggg cgatggcgcg ctcaggttca tcgccgccgt cctagcagtg gctatcgggc
      121 tttcgctccg gtctgcccga aggctcgagc cgccggagga tcttgcggca cccgaccctt
      181 cgggctactc acccgggcga cgccagccag gaagcgagag gggacgggcg tccgagggta
      241 gcgtcctgcg gcatggatgt tgcatccaac gagggcggcg gattggtgac ctcgattgcg
      301 ggtacgcgcc cgacgcccgt atactcttcg ccaaccgccc atgagtgcgg aatccgctgg
      361 aatcgttacc ctgagcaagc ctcgcaccgt cgccatcgat ggacctgtcg cctcgggaaa
      421 gagtagcgtt ggcagattgc ttgcacagcg gtggggccat gtattcctcg acacgggcat
      481 gatgtaccga gccatgacgt ggtgggcgct tcgccatggc atcgcgccgg acgacgccgc
      541 ggcgcttgcc cggctcgctt ccgaggcccg catcaccatc gaaccaaccg ccgaggttcc
      601 gcgcgtcatg atcgatgggg aggacgccac gcctcacatg cgagaccgcg aggtcgaggc
      661 caacgtatcc atggtgtcgg cggtgccagg cgtgcgtagc gcccttgtgc gcgagcagcg
      721 ccgcatcgcc gagcagcagc ccgtcgtcat ggccggacgc gacatcggca ccgtcgtcct
      781 gccggatgct gacctcaagg tgtacctcga tgcctcggtc gaaacgcggg cccggcgccg
      841 ccatggcgag ctcgatcgcg gctcccgcac gctcgaggaa gttcgggagg agcttaccgc
      901 ccgcgatcgc ctcgattccg agcgctcagt ctcgccactt cgccctgccg acgatgccat
      961 aatcctcgac gtcgaccacc ttgggctggc cgaggtcgtc gagcgcatcg cggcattcgc
     1021 tggttgcgag ggcgatggca cagcggaggc cacctcgtga cggccgcccg tccgggactc
     1081 gtcgtacgcg tatgcgcggg catcgtgaac actgtcgtgc gcgggctggc gttcgccttc
     1141 acgcgctgga gggtggaggg gcgcgagcac gtgccccgcg aaggctctgt catccttgtc
     1201 gcgaaccaca ttaccaactt ggacccgccc atcctcgcca cggcgacgca tcgccatgtt
     1261 acgtacatgg cgaagcagga gctgttcgat ctgccggtca tcggcttcgt gttccgcctg
     1321 tacggggctt tcagcgtgcg ccgcttcgag gccgacctcg cggcgttgcg tcaggcgcag
     1381 aacgtcctca aaggggggca ggtcctaggc atgttcccgg agggcacgcg gagccgcggc
     1441 agcggcctcg gcgctccgta tcccggggcc gcgctcatcg ccctccgttc gaacgcgctc
     1501 atcgtgccgg ccgccatcac gggcacggaa cgcttgatgg gattgggact gatactgttt
     1561 ccgttcaccc ggccccgcat caccgtacgc ttcggcgagc cgttccgcct gccgcagacg
     1621 gcgcgtccca ggcctgacga catcaaacgc gggtcgatgc agatcatgcg gtccatcgcc
     1681 gagctcctgc cggagccgta cctggggccg cacgggcgcg ccctcctcgc cggcgaggac
     1741 attcctctag ccgcggggcc gccggcgcac tacctggaga agactgccgg gcagcgataa
     1801 cggacgaatc gcgggacgct cacgagatca ggctacgagc gccgactcgg tatccagcgc
     1861 ctgcacgaat gcgtcaacga gccgcgaatc caggcgcgtg cccgcagcgg cgcgcaactc
     1921 gcgaatcgcg tcggcgtgag agggaacgtt gccggatgcc cgacccgcac gaatagtgac
     1981 gtaggcgtcc gcgactgaca ggatgcgcgc cgaaaccggt atctcgtcgc ccttcaggcc
     2041 gcaaggatac ccgctgccat cccagtgctc gtggtgcgcg aacgcagccg tcgcagccgg
     2101 ggcgagcggt gggctggcct cgaggatgcg ccgcgagacg tgaacgtgct ctcggacaac
     2161 ggcaagctcc tcggctgtga gctccgaggc cttgtgaagt agcgcgtccg gcaccacgag
     2221 gttgccgatg tcgtgcaaaa gggccgcgat ctttagcgcg aggtgttgct ccttcggcag
     2281 ctcgacggcc ttggccaaca gcagtgcgta gtgagcaact tggcgtgcct gctcgcgcgc
     2341 gtctggcatc ttggaagaca gcgcttccag gagtccattg acggtggcag tctgcttcgc
     2401 gagttgctcg ccatacgcga cgacgacctg tcctccgttg gtggcggcca gccattgcgc
     2461 ctcggaggcg cggcgcacga cctcgtcggc atgctccgcc ccgctggcgg tggccacgcc
     2521 gacgctcatc gagatgggaa cgagcggccg gggcccggac accggcagac cgcggcggag
     2581 cccctcgtgg aggagcctac gcccgagctc gcgcgccgtc ctca
//
LOCUS       DATMBB010000055         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_39189, whole genome shotgun sequence.
ACCESSION   DATMBB010000055 DATMBB010000000
VERSION     DATMBB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39189"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VNL92_01145"
     CDS             complement(<1..1002)
                     /locus_tag="VNL92_01145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWO93347.1"
                     /translation="MVELGEATPSGRGQIRWEAPNTRRIERAALAYRRILPLLVTGFA
                     LMLVSLGDRPAQAEHSLSFPFASLDGWYVQQGYDQGSHSGYERYALDLSRDPDPTNTV
                     VLAPASGTVAWIDPGYGCLSLSLGHDGLHLSICHLKLVPAFATGESVAAGQIIGAVAP
                     AGEAGNNGMPHVHMALYRCIDDTCASSAAGREPVPFAGEHALSGVSFEAGDDHTSKLL
                     AALVADPSVAPEAGIDADPAAPSTSAGELAYPPQELLAGVNPIIYFGPALPPKQAFGN
                     LGDAYVAAFAWDAASQRWLIDVPLLAGEGALTHVQPFGAYWIVTSAPATLEPQTFDEP
                     "
     gene            1116..>2002
                     /locus_tag="VNL92_01150"
     CDS             1116..>2002
                     /locus_tag="VNL92_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93348.1"
                     /translation="MASLFSEPIVCPLLVGREDAQSTLRRLLAGVAERGRVVLVSGEA
                     GIGKSRLLADFSDEARRAGHLVLGADFFEQEERVPYAAIGRLLGGLAAEVDTPEARIL
                     ESLAHDLIPVAPRLSSLAISSRERVPQTSDFDQDGQQISDALISLVRALAEGRTMLMV
                     FEDLHWADSSSLCALLRIGRDAPLGCLLVLSYRDDETTSQLQSFLATLDRERLGAELH
                     LGRLGLGDVDRMLTAIHGSEKRVRADVLHMVYQLTDGNPFFVEELVRSLVDHAGSVEA
                     LSAGRLAQVDIPRGVNEALR"
BASE COUNT          323 a          616 c          698 g          365 t
ORIGIN      
        1 tggctcgtcg aaggtctgag gctcgagcgt cgcgggtgcg gacgtcacga tccagtaggc
       61 gccaaacggc tgcacgtgcg taagggcgcc ctcgccggca agcagcggaa catcgatgag
      121 ccagcgttgg cttgcggcat cccaggcaaa cgccgccacg taggcatctc cgaggttccc
      181 gaaggcctgc tttggcggaa gcgcgggtcc gaagtagatg attgggttga cgccggctag
      241 cagctcttgc ggcgggtagg cgagctcgcc cgcggatgtg cttggggccg ccgggtccgc
      301 gtcgatgcca gcctcaggcg cgaccgaggg gtccgcaacg agcgcggcga ggagcttcga
      361 tgtgtggtcg tcgccggctt cgaacgatac gccggagagc gcatgctcgc cagcgaacgg
      421 aaccggctcg cgtcctgcag ccgaggatgc gcaggtgtcg tcgatgcagc ggtacagggc
      481 catatggacg tggggcatgc cgttgtttcc ggcctcgccc gctggggcca ccgcgccgat
      541 gatctggccc gccgccacgc tctctccggt agcgaaggcg ggcacgagct tgaggtgaca
      601 gatgctgagg tggagcccgt cgtgcccgag gctcaggctc aggcagccgt aacccggatc
      661 gatccacgcg acagttccgc tggcgggtgc gaggaccacg gtgttcgtcg ggtcggggtc
      721 gcgcgagagg tcgagggcgt agcgctcgta cccggagtgg cttccttggt cgtagccctg
      781 ctgcacgtac cagccgtcga gcgaggcgaa ggggaagctc agggaatgct ctgcctgcgc
      841 gggtcgatca ccgagcgaca ccagcatcag ggcgaagccg gtgacaagca gcggcaggat
      901 gcggcggtag gccaacgcgg ccctttcgat gcggcgcgtg ttcggagcct cccagcgaat
      961 ttgccctcgc cccgagggag ttgcctcgcc cagttctacc atggctaacg cttacgcgga
     1021 acagaaggtt cgccgccgag cactggtaac cgttcgccgc ttaggagtgg ctgcgaggat
     1081 acgtgcgctg cgagacatgc tatcgttttc ccgaaatggc gtcgcttttc tcggagccga
     1141 tcgtttgtcc gctcctcgtc ggccgcgagg acgcgcaatc aaccctgcga cgacttctcg
     1201 caggcgtggc cgagcggggg cgcgtcgtgt tggtttcggg tgaggccgga atcgggaaga
     1261 gtcgcttgtt agctgacttt tccgacgagg cgcggcgtgc tgggcatctc gtgctcggcg
     1321 cggacttctt cgagcaagag gagcgagttc cctacgcagc gatcggacgg ttactcggtg
     1381 ggctcgcggc ggaggtagac actcccgaag cccgcatact cgagtccttg gcacacgatc
     1441 tcataccggt cgcgccgcgc ctctcgtcac tcgccatctc aagtcgagag agggtgccgc
     1501 agacgtctga cttcgaccag gacgggcaac agattagcga cgcgctcatc agccttgtgc
     1561 gcgctctcgc tgaaggccga acgatgctga tggtcttcga ggacctgcac tgggcggatt
     1621 cgtcgagcct gtgcgcgctc ctgcgtattg ggcgcgatgc gcctttgggt tgcctactgg
     1681 tgttgagtta tcgggatgac gagactactt ctcagctgca atccttcctc gctacgctcg
     1741 accgcgagcg gctgggcgcg gagctccacc tcgggaggct cggcctcggc gacgtggatc
     1801 ggatgctgac agcaatccat ggctccgaga aacgcgtccg cgcggatgtt ctccacatgg
     1861 tctatcagct gaccgacggc aacccgttct ttgtcgagga actggtgcgt tcgctcgtcg
     1921 accacgccgg cagcgttgag gcgctcagcg ccggacgtct cgcccaagtc gatatacctc
     1981 gcggagtcaa cgaggctctg cg
//
LOCUS       DATMBB010000056         2699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_309554, whole genome shotgun sequence.
ACCESSION   DATMBB010000056 DATMBB010000000
VERSION     DATMBB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309554"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1380
                     /locus_tag="VNL92_01155"
     CDS             70..1380
                     /locus_tag="VNL92_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93349.1"
                     /translation="MSWAEGKRILVLSRAVVGERMSSPGIRAANMARVLAEALPEAHI
                     TLGVPANSIGPPREEAYETALYRIRTLAGLVARNDVIISMGFPPHLVPLFMRKTLVLD
                     FFSQFYVEWTELERGKGPSWTRRMRGNSNRSFIAEQLTFADFILCANERQRDSYLGAL
                     SALGLLDASVYERDRSLRSLIDVAPHGVRPEPPVHSGTRRLKGVHPAVRESDRVFVWN
                     AGIVPWYDPVTLIRAIARIRERRDDVKLLFLGTRYPDAVWEENRALHNAFALARDLNL
                     IDETVIFQDGWVPFEEVKDYILECYAGACTYRDTLETRYSFRTRFVDLVWAGLPIVCT
                     AGDVLAERVDRETLGIVVPEGDVDAVEAALLRLLDDCAFYETCRANLACVREELTWER
                     AFAPLVRFLHEGVSGAKPKRSRLGPLVARSAHYLADRSALTLIR"
     gene            1395..>2699
                     /locus_tag="VNL92_01160"
     CDS             1395..>2699
                     /locus_tag="VNL92_01160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93350.1"
                     /translation="MVLSRGQVGREMSSPGIRAYHMARVLAQALPNDEVTLAAPNVID
                     VPPPHPRVRLAQVGGFNLREMARHDIIISRNFHPQAVLLFLRRTFILDLFSMLNFEWM
                     EFGFGQRDKLRRSLWLGANNRYLNLQLTFADYAIAATEHQRDHWIGMMWALGLVNPDV
                     YERDPTLEKFVGLVPYGVRPEPPLEQVGREALCRAFPGIRPDDTVLIWNGGIVSWYDP
                     CTAVRAVDRIRRYRDDVKLVFLGLAYPFGGDFELGPEVREAITLARDLGLEGSHVFFN
                     FGWVPYDETMRLVQEADIGLCTAREGMEARYAYRTRFVDLIWAGLPIVCTQGDALAER
                     VASEPWGVTVAEGDADGVVSAVLRLLDDGAFAEECRQNLMRAKDEMQWERTLEPLVDF
                     MRNGHSVARAKVTRLPALLMRMAEYLALRGLGSLYGRRRSSTA"
BASE COUNT          412 a          903 c          890 g          494 t
ORIGIN      
        1 caccaccgcc ctctctgatg cggtttcgtt cgcgctgttc agcgtggtgg tcctccgcta
       61 cctgcgttca tgagctgggc tgaagggaag cgcatcctcg tgctcagccg cgccgtagtc
      121 ggcgagcgga tgtcgagccc cggcattcgg gcggccaaca tggcccgcgt gttggcggag
      181 gcgctgccgg aagcgcacat cacgctgggc gtacccgcga actccatcgg cccgccccgc
      241 gaggaagcgt atgagacggc gctgtaccgc attcgcacgt tagccggcct cgttgccagg
      301 aacgacgtca tcatctcgat ggggttcccg ccccacctcg tgccgctctt catgcggaag
      361 acgctcgtgc tcgatttctt cagccagttc tacgtggagt ggaccgagct ggagcgcggc
      421 aagggaccgt cgtggacgcg ccgcatgcgc ggcaactcga atcgcagctt catcgcggaa
      481 cagctcacct tcgccgactt catcctctgc gcgaatgagc gtcagcgcga tagctacctc
      541 ggtgccctgt ccgcgcttgg actgctcgat gcgagtgtgt acgagcgcga ccgctcgctc
      601 cggtcgctca tcgacgtggc gccccacggc gtgcggcccg aacctcccgt ccactccggc
      661 acgcgccggc tcaagggcgt tcaccccgcc gtgcgcgaga gcgaccgtgt cttcgtttgg
      721 aacgccggca tcgtgccctg gtacgacccg gtgacgctca tccgggccat cgcccgcatc
      781 cgcgaacggc gtgacgacgt caagctgctg ttccttggaa cgcggtaccc cgatgccgtg
      841 tgggaggaga atcgagccct ccataatgcc ttcgccctcg cgcgggacct gaacctcatc
      901 gacgaaacgg tcatcttcca ggacggctgg gtgcccttcg aggaggtgaa ggactacatc
      961 ctcgagtgct acgcgggggc ctgcacgtac cgcgatacat tggaaacgcg ctacagcttt
     1021 cggacccgtt tcgtcgacct cgtttgggcg gggcttccca tcgtctgcac tgccggcgac
     1081 gtcctcgcgg agcgcgtcga ccgcgagacg cttggcatcg tcgttcccga gggcgatgtc
     1141 gatgcggtgg aggcggcgct cctgcgcctg ctcgacgatt gcgccttcta tgagacctgc
     1201 cgggcgaacc tggcctgcgt tcgcgaggag ctcacctggg agcgcgcgtt cgctccgctg
     1261 gtccgcttcc tgcatgaggg cgtctcgggt gccaagccca agcggagcag gctggggccg
     1321 ctcgtcgcgc ggtctgcgca ctacctcgcc gaccgctcgg ccctcaccct gatccgatga
     1381 gccgcaggcg aatcatggtt ctcagccggg gccaagtcgg gcgcgagatg tcgagccccg
     1441 ggatccgcgc ctaccatatg gcccgggtgc tcgcgcaggc gctgccgaac gacgaggtca
     1501 cgctcgcggc gcccaacgtc atcgacgtgc cgccaccgca cccgcgcgtg cgcctggcgc
     1561 aagtcggcgg attcaacctg cgggagatgg cccggcacga catcatcatc tcccggaact
     1621 tccaccccca agcggtgctg ctcttcttgc gacgaacgtt catcctggat ctgttctcga
     1681 tgctgaactt cgaatggatg gagtttggct tcggtcaacg ggacaagcta cgccgcagcc
     1741 tctggctcgg cgcgaacaac cgctacctca acctgcagct cacgttcgcc gattacgcca
     1801 tcgcggcgac tgagcaccag cgcgaccact ggatcggcat gatgtgggcg ctgggcctcg
     1861 tgaacccaga cgtgtacgaa cgcgacccga cgctcgagaa gttcgttggt ctcgtgccgt
     1921 acggcgtccg gcccgagccg ccgctcgaac aggtcggtcg cgaagcgctg tgccgagcgt
     1981 ttcccggaat tcgtccggac gataccgtgc tcatctggaa cggcggcatc gtcagctggt
     2041 acgacccgtg caccgccgtt cgcgccgtgg accggattcg acggtaccgc gacgacgtga
     2101 agctcgtgtt cctcgggctc gcttacccgt ttggtggcga cttcgagctc ggtccggagg
     2161 tgcgggaggc gataacgctc gcacgcgacc tcggcctcga gggatcgcac gtgttcttca
     2221 acttcggatg ggtgccgtac gacgagacga tgcgccttgt ccaggaggcg gacattggcc
     2281 tgtgcacggc gcgcgagggc atggaggcgc gctatgctta tcgcacgcgc ttcgtcgacc
     2341 tcatttgggc cggcctgcca atcgtctgca cgcagggcga tgctcttgcg gagcgcgttg
     2401 catcggagcc gtggggcgtc accgtggcgg agggcgatgc cgatggggtc gtgagcgccg
     2461 tccttcgcct cctcgacgac ggcgcctttg cggaggagtg ccggcagaat ctgatgcgag
     2521 ccaaggatga gatgcagtgg gaaaggaccc tcgagcccct cgtcgacttc atgcgtaacg
     2581 gacacagtgt ggcccgcgcg aaggtgactc gtttgccggc tctcctgatg cggatggcag
     2641 aatatctggc gctgcgcgga ctgggctcgc tgtacgggcg gcggcggtca tcgacggcc
//
LOCUS       DATMBB010000057         2764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_587755, whole genome shotgun sequence.
ACCESSION   DATMBB010000057 DATMBB010000000
VERSION     DATMBB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587755"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VNL92_01165"
     CDS             <1..841
                     /locus_tag="VNL92_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWO93351.1"
                     /translation="HGKLLQARSGAERDREALERCRSDYGEWERRRHEAQCEVEGLHE
                     AAQSARQAAERLADAERIRDETKAHLGRLRQALGAVEGEISKSASLLADRPAKAEQLR
                     AVAEEASLYEELCAAFGRRGVQALLIDSALPELMEEANRLLHRMTAGRMSLFITTQRA
                     TQKGELAETLDIQIADELGTRAYEMYSGGEAFRIDFALRIALSRLLARRAGAPLPILV
                     VDEGFGSQDATGRERLVEAITAVQDEFRMILAITHIDELRDVFPHRIHVTKTPHGSQL
                     EVL"
     gene            complement(922..1200)
                     /locus_tag="VNL92_01170"
     CDS             complement(922..1200)
                     /locus_tag="VNL92_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWO93352.1"
                     /translation="MTYVITQPCIGTKDASCVEVCPVDCIHSEDEDAIYYIDPAECID
                     CGACEPVCPVTAIFAEDDMPDDQAEFKEINALWFQDKDAARARVAAYT"
     gene            complement(1310..1792)
                     /locus_tag="VNL92_01175"
     CDS             complement(1310..1792)
                     /locus_tag="VNL92_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWO93353.1"
                     /translation="MTESGGREPRRPAVTKAAGRGKGSSRCSDPPPDGLLRAIDQFNR
                     GEYWEQHETLEDIWIEEADPVRHLYQGILQVGVAFHHWRRSNFRGADLLLERGLTYLQ
                     PFRPSCMGVDVERLVMEAAACHAELRRLGPRRMALFDPARFPRVHLVGESETTRDISE
                     "
     gene            complement(1843..>2764)
                     /locus_tag="VNL92_01180"
     CDS             complement(1843..>2764)
                     /locus_tag="VNL92_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWO93354.1"
                     /translation="LSPASRPDDMTDQRHRCGAPDAMGYAVPARRVAGTGDAMPETPD
                     LEAIRGFFNARILGRTIVEARILIPVVARFPREEFAALAGDQFAHIDRRGKFLLFRTA
                     SGRILVINAMLTGRFQYVDSGEKRRAKTCAVITLDDGCDVRYADERLMGKLYLATEAT
                     LAELPQFGEMGPDVLDPALTEDVFLARLRRHRGELKGILVNHRFVAGIGNAYADEVLW
                     EAGLHPYRKRTALSDEGARRLYEAMRAVMDWAIPILAEKAKDQLPYEEWRDHMRVHRK
                     GGKPCPRCGTTISEVTAGQRITNFCRTCQS"
BASE COUNT          427 a          903 c          975 g          459 t
ORIGIN      
        1 gcacggcaag ctccttcagg ctcgcagcgg ggccgagcgc gatcgggagg cgctggagcg
       61 ctgccgcagc gactacgggg agtgggagcg gcgcagacac gaggcgcagt gcgaagtgga
      121 ggggctgcac gaggccgccc aatcggctcg tcaggctgcc gaacgcctcg ctgacgcgga
      181 gcggatccgg gacgagacga aggcccactt gggccgtttg cgccaggctc tcggcgccgt
      241 ggagggcgaa atcagcaagt ccgcaagcct cctcgccgac cgtcctgcca aggccgagca
      301 gctccgcgcc gttgccgagg aggcgagcct ctacgaggaa ctctgcgccg cgttcggccg
      361 gcggggcgtg caggcgctcc tcatcgattc ggcgctcccg gagctcatgg aggaggcgaa
      421 ccggctgctc caccggatga cggcgggacg gatgtcgctc ttcatcacca cacagcgggc
      481 gacgcagaag ggcgagctcg ccgagaccct cgacatccag atcgcggacg agctcggcac
      541 gcgcgcctac gagatgtaca gtggcggcga ggcgtttcgc atcgacttcg ccctaaggat
      601 cgcgctgtcg cgtctgctcg cgaggcgcgc cggtgcgccg cttcccatcc tcgtcgtcga
      661 cgaggggttc gggtcgcagg atgcgacggg tcgcgagcga ctcgtggagg cgatcacggc
      721 cgtccaggac gagttccgca tgatcctcgc catcacccac atcgacgagc tccgcgacgt
      781 cttcccgcac cgcatccacg tcacgaagac gccgcacggc tcgcagctgg aagtgctgta
      841 gccgcctcgc gataccgccg gcgacgaacg aaggcccagc gatgtcgccg ggccttcgct
      901 ctgattttcg gaccgatgcg tttaggtgta ggcggcgacg cgggcgcggg cggcgtcctt
      961 gtcctggaac cagagcgcgt taatttcctt gaactcggcc tggtcgtcgg gcatgtcgtc
     1021 ctccgcgaag atcgccgtca cggggcagac gggctcgcag gcgccacagt cgatgcactc
     1081 ggccgggtcg atgtagtaga tcgcgtcctc gtcctcgctg tgaatgcaat cgaccgggca
     1141 gacctcgacg cacgaggcgt ccttcgtgcc aatgcagggc tgggtgatga cgtaggtcat
     1201 ggctggtggt ccccttgctg tatggtggcg gggcgcgagc cgccgcctcg tgcctcattg
     1261 tagtgaggac cgacctggtg tcaactactt atgacagtga tggcttcgtt tattccgaga
     1321 tgtcgcgcgt tgtctccgac tcgccgacga gatggacgcg cgggaagcgc gccgggtcga
     1381 agagcgccat gcggcgcggc ccgaggcgcc ggagctcggc gtggcatgct gcggcctcca
     1441 tgacgagacg ctcgacatcg acgcccatgc atgacgggcg gaacggctga agataggtca
     1501 gaccccgttc gaggagcagg tcggcgccac ggaagttgga ccgtcgccag tggtggaagg
     1561 cgacgcccac ctggagaatg ccctggtaca ggtggcggac ggggtcggcc tcctcgatcc
     1621 agatgtcctc gagcgtctca tgctgctccc agtactcgcc gcgattgaac tgatcgattg
     1681 cgcggaggag gccgtccggg ggcgggtcgc tgcaacggct acttcccttg ccgcgaccgg
     1741 cggccttcgt gactgcgggt ctcctgggtt cgcgcccgcc gctttccgtc atggtgacct
     1801 ccgcgccact tgtatcctat cggaccacat gcccgatgac ggttacgact ggcaggtgcg
     1861 gcagaagttc gtgatgcgct ggccagccgt gacctccgat atggtagtcc cgcagcgggg
     1921 gcaaggcttg ccgcccttgc ggtggacgcg catgtggtcg cgccactcct cgtagggcag
     1981 ctggtccttc gctttctcgg cgaggatcgg tatcgcccaa tccatgacgg cccgcatcgc
     2041 ctcgtagagc cgccgggcgc cttcgtcgct cagcgccgtc cgttttcggt aggggtggag
     2101 ccccgcctcc cagaggacct cgtccgcgta ggcgttcccg atgccggcga cgaaccgatg
     2161 gttcaccagg atgcccttca gctcgccacg atggcggcgt agccgtgcga ggaacacgtc
     2221 ctccgtgagc gccgggtcga gcacgtcggg tcccatttcg ccgaactggg ggagctcggc
     2281 gagcgtcgcc tcggtcgcga ggtagagctt ccccatcagg cgctcgtcgg catagcgaac
     2341 gtcgcagccg tcgtcgaggg tgataacggc gcaggtcttg gcgcgtcgct tctcgcccga
     2401 gtcgacgtac tggaagcggc ccgtgagcat ggcgttgatg acgaggatgc gccccgatgc
     2461 cgtgcgaaag agcaggaact tgccccgccg gtcgatgtgg gcgaactggt cgccggccag
     2521 cgccgcgaac tcctcgcgag ggaagcgcgc gacgacgggg ataaggatgc gcgcctccac
     2581 gatggtgcgg ccgaggatgc gggcattgaa gaacccgcgg atcgcctcga ggtcgggtgt
     2641 ttccggcatc gcgtctcccg tcccggcgac gcgccgagcg ggcacagcat accccatggc
     2701 gtccggcgcg ccacaccggt gccgctggtc cgtcatgtcg tctggccgcg acgcggggct
     2761 caga
//
LOCUS       DATMBB010000058         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_21394, whole genome shotgun sequence.
ACCESSION   DATMBB010000058 DATMBB010000000
VERSION     DATMBB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21394"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VNL92_01185"
     CDS             complement(<1..790)
                     /locus_tag="VNL92_01185"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWO93355.1"
                     /translation="MVTLRELFGIELPIIQAPMASAQDSALAIAVTNAGGLGSVPCAL
                     LSPDGVRAELSTMRQHARGPFSVNFFCNAPPKRDESRDAAWRAALAPYYREYEMEPGD
                     PGPGRGGGPFSADAVDVLEEFRPAVVSFHFGLPPDNLLGRVKSLGAKVIASATTVDEA
                     MWLEARGVDAIIAQGVEAGGHRGMFLTDDITTQVGTLALVPQIASAVGVPVIAAGGIA
                     SGRGVAAAMALGAAGVQVGTAYLLCQEALTSPMYRAALSSTAARH"
     gene            complement(943..1794)
                     /locus_tag="VNL92_01190"
     CDS             complement(943..1794)
                     /locus_tag="VNL92_01190"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HWO93356.1"
                     /translation="MSVPLRVGHSPDADDAFMFYGIAKGVVDTAPYVIEHVIEDIQAL
                     NERAMRGDLEVTAISAAVYPQVAGRYRILACGASIGRGYGPIVLARPGEGGSIAGRRI
                     AIPGEHTTAYLLLRIFERDFVPVIMGFDEVLPALDARRVDAALVIHEGQVTYKAGGYE
                     SLVDLGVVWERETGLPIPLGLDVVRRDLGEDAALAIYRALRDSILHTNEDEEGAIDYA
                     LRFGRGIDRETCRTFVRMYVNRDTVQMGDEGRRALDELYGRALRRGLISEMPPLDIVG
                     LEAAPKG"
     gene            1992..>3026
                     /gene="mqnE"
                     /locus_tag="VNL92_01195"
     CDS             1992..>3026
                     /gene="mqnE"
                     /locus_tag="VNL92_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HWO93357.1"
                     /translation="MVSTLHPDDVTFRDVALAPIWDKVRRGERLSLEDGLQLYDSPDF
                     IAVGRMADYVKRQKSGDEVFFVFNRQVNPTNLCVLSCAFCDFAAKPGDAHAYELSLDE
                     ILASLSDEITEVHIVGGHHPTWPFERYEDILRAIHERYPDVQIKAFTAAEYDYFEKRW
                     KVPVPEALARFKALGLRALPGGGAEVFSDRVRKLLFPGKVSAQRWLEIHRIAHQMGLK
                     SNATMLYGHIETYEERLRHMVLLRELQDETGGFQVFIPLEYQINENHLATRQASAIDN
                     LKTIAISRLMLDNIPHIKAYWIMMGEEAAGMALNFGADDLDGTIGHERIAHAASATSP
                     IGLARERLVRL"
BASE COUNT          511 a         1047 c          955 g          513 t
ORIGIN      
        1 tgtgccgggc tgccgtactg ctcaacgccg cccgatacat cgggctggtc agcgcctctt
       61 ggcagagcag ataggccgtg cccacctgga cacccgccgc gccgagagcc attgcggcgg
      121 cgacgcctcg cccgctcgcg atgccccccg ctgcgatgac gggcaccccg accgcgctcg
      181 cgatctgcgg cacgagcgca agtgtgccaa cctgcgtcgt gatgtcgtcc gtgaggaaca
      241 tgccccgatg gccgcccgct tcgacccctt gcgcgatgat cgcgtcgacg ccccgcgcct
      301 cgagccacat cgcctcatcc acagtcgtcg ctgaggcgat gaccttggcg ccgagcgact
      361 tcacgcgtcc gagaaggttg tccggcggta acccgaagtg aaagctgacg acggcgggcc
      421 gaaactcctc gagtacgtcg acggcgtccg cggagaacgg tccgcctccc cgaccggggc
      481 ctgggtcgcc gggctccatc tcgtactcac ggtagtaggg cgcgagcgcc gccctccagg
      541 cggcgtcgcg gctttcgtcg cgcttcggtg gggcgttgca gaagaagttg acgctgaagg
      601 gccctctcgc gtgctgccgc atcgttgaca gctcggcgcg aacgccgtcg ggcgagagga
      661 gcgcacacgg caccgagccg agcccaccgg cattcgtcac agcgatggca agcgcgctgt
      721 cctgcgcgct tgccatgggc gcctggatga tcggcagctc gataccgaac agctctcgca
      781 gcgtgaccat accgcctccc gtcctgcgcg ccgcattggc atcggcgagc ggcgagcgtg
      841 gcgcgcggat cggacgccgc ttctgccatc ctgccaccgc ccagcagtca catcaagttg
      901 ctgcactcgt cagcccttgg gcggacaacc cccgcagccg cctcagccct tgggcgcggc
      961 ttccaggcct acgatgtcga ggggcggcat ctcggagatg aggccccggc gtagggcgcg
     1021 cccgtacagc tcgtcgaggg cgcgccggcc ttcatcaccc atctggaccg tatcgcgatt
     1081 gacgtacatg cggacgaacg tccggcacgt ctcgcggtcg atgccgcgcc cgaaccgcag
     1141 cgcgtagtcg atggccccct cctcgtcctc gttcgtatgg aggatgctgt cgcgcagggc
     1201 tcgatagatg gcgagcgccg cgtcctcgcc gaggtctcgc cgcacgacgt cgaggcccag
     1261 cgggatgggc aagcctgtct cgcgctccca gaccacgccg aggtcgacga ggctctcgta
     1321 tccgcccgcc ttgtaggtga cctgaccctc gtgaatcacc agcgcggcat ccacgcgacg
     1381 tgcgtcgagc gcgggtagga cctcgtcgaa tcccatgatc acgggaacga aatcccgctc
     1441 gaagatgcgc aacagcaggt aagcggtcgt gtgctcgccg gggatcgcga tgcggcggcc
     1501 ggcgatgctt cccccttcgc ctggcctggc aagcactatc ggtccgtatc cacgaccgat
     1561 cgatgctccg caggcgagga tgcggtagcg gccggcgact tgggggtaga ccgccgcgga
     1621 gatcgctgtc acctcgagat ctccccgcat cgcccgctcg ttgagcgcct ggatatcctc
     1681 gatgacgtgc tcgatgacgt aaggcgccgt atcgacgact cccttggcaa tcccgtagaa
     1741 catgaacgcg tcgtctgcat ccgggctatg tccaacgcgc agcggaactg acacgaacct
     1801 cactcctctc gatctctgac tgcggaggct cgcgagccgc agacggatag gcccgcgccc
     1861 atcggcgagc catcttcgac cttcattgcg cgagctagca cggagccgcg aacgtcacta
     1921 tactacgaga tgctgttcag gcacccggcg cagtagccgg ctttcgcatc cccaagaagg
     1981 atcccgacgc gatggtatcg accttacacc ctgacgatgt cacgttccgc gacgtcgcgc
     2041 ttgctccgat ttgggacaag gttcgtcgcg gcgagcggct gtccctcgag gatgggctgc
     2101 agctctacga cagcccggac ttcatcgccg tcggacggat ggccgattac gtgaagcggc
     2161 agaagtccgg cgacgaggtg ttcttcgttt tcaaccgcca ggtcaacccg accaacctgt
     2221 gcgtcctctc gtgcgcgttc tgcgacttcg cggcgaagcc tggcgacgcg cacgcgtacg
     2281 agctgagcct cgacgagatt ctcgcctcgt tgtccgacga gatcactgag gttcacatcg
     2341 tcggcgggca tcatcctacg tggccgttcg agcggtacga ggatatcctc cgggccatcc
     2401 acgaacgtta cccggacgtg cagatcaagg cgttcaccgc ggcagagtac gactacttcg
     2461 agaagcgctg gaaggtgccc gtccccgagg ctctggcccg gttcaaggct cttggcttgc
     2521 gggccctccc gggcggcggc gccgaggtct tctcggatcg cgtgcggaag ctgctcttcc
     2581 ctggcaaggt cagtgcccaa cggtggctgg agattcatcg catcgcccat cagatggggc
     2641 tgaaatccaa cgccacgatg ctctacggtc acatcgaaac ctacgaggag cggctccgcc
     2701 acatggtctt gttgcgggag ctccaggacg aaacgggtgg attccaggtc ttcattcccc
     2761 tcgagtacca gatcaacgag aatcacctcg ccacgcgcca agcctcggcc atcgataacc
     2821 tgaagaccat cgcgatttcg cgccttatgc tcgacaacat cccgcacatc aaggcctact
     2881 ggatcatgat gggcgaggag gcggcgggga tggcgctcaa cttcggcgcc gacgatctcg
     2941 acggcaccat cggccacgag cgcatcgccc acgcggcgag cgccacgagc cctatcggtc
     3001 tcgcgcgcga gcggctggtg cggctc
//
LOCUS       DATMBB010000059         9489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_226631, whole genome shotgun sequence.
ACCESSION   DATMBB010000059 DATMBB010000000
VERSION     DATMBB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226631"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VNL92_01200"
     CDS             <1..431
                     /locus_tag="VNL92_01200"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018720368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWO93358.1"
                     /translation="KFRSMVDGADGLQIALARFVGQDGQPLFKLPDDPRVTRVGRLLR
                     RTSLDELPQLINVLRGDMSLVGPRPPLPGEIACDRRHLARLEAVPGITGLWQVSGRST
                     LSFEDMVGLDLRYINGWSFTLDLSILFRTIPAVLSRRGAC"
     gene            479..1894
                     /locus_tag="VNL92_01205"
     CDS             479..1894
                     /locus_tag="VNL92_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWO93359.1"
                     /translation="MPEQTRLALYKYLAILARRKWVILTPVVVGVLGAVAATFWLPQA
                     MPRTYEATATAQLAVAGRSEKGLSDINKEQADRLTNTFVYVLQGPDVLSDVIADLGLD
                     ATPSDLKSRLTFEPVAGTELVEIKARAPSPEEARDIANAMATAWREQGSAFYAILDTP
                     ELSRSFTVAEEASLPASPIEPDWQQRVGLGLLMGLVAGTLLALAIEYTDRTINSPSDL
                     RDLVEVPVLSHLPRVPKLLGSTRRRIAELDVPPYQYTFDSGELRLLTIKLLHTLQDQH
                     LNSLFVTSTWPSEGTTTVAIGVAMGLARSGVEVLLIDANLSRPALHTAFGGIPEAPGL
                     TDVLLSMGEDEPPQALLRKTIHEPPVDGLSVLTAGSPHADSWNILGSGEMRKLVESYA
                     GTDTVLVIDGPPVLSSVDALGAAAMAAGVVLVCAEGKTSVDDVQRGLSQLALLGANVL
                     GLVYNQARTPIGTIARSAAAV"
     gene            1990..4797
                     /locus_tag="VNL92_01210"
     CDS             1990..4797
                     /locus_tag="VNL92_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWO93360.1"
                     /translation="MSNAQARVARNTLLQLAGRLMTSLLSLAAVSILARSLGAEEVGS
                     YLLVLSLLAMLNLSDLGFSTIGIRELAARPDEQEKLLGEFIVIRIGLALLAMVIVSIA
                     ALLLDFGSAVTTAIWLGSLTYLFTALGPATFGLTFAANLRMEYQVAANVIQAGVLVGL
                     VAMLGLLGGGIVAAVLAYDASVFVGSIMTVILASRFVLPRLSLDIRRHWWLLRESAPM
                     GLAALCWLTYNRIDMVLISKLADEEALGLYSVAYKSVDLAWPIGLSFALSAFPLLTRH
                     FRAGDTAEFQRLLQKSFDMLSVLMIPAVTFLIVFAEQILVLVGSDDFRPAATSLRLLA
                     LAIGVMLVSVLMSHALMAAGRQSGFFRAMPIWVCINIGLNLFLIPWLGFNGAAIATLL
                     TELSILLCVLVLLARHVHHVPSFAVPARMMAVALATVAALLALPDDPAFRIVFLPFAF
                     IVGTALTRAVAIADLREIVRRERVAGDRPGLDARSRPPVWPMRAPGEENGRLPGRLGN
                     AANGAAINGSETTKPSDPSDRALEGRDIVCLSFSAWDDHWGTPQQLISRLGKRNRILY
                     VEPPVSPMSFFTGLRRKSFVWQRLRYSRVGPRHVDAGIWVAAPPMALPLRYTRPVSRA
                     NALIMRRWLAKQVEELGLRDVILWNFQPLMAGVAGAVNPALTIYHCVDDFTSVPKWWN
                     RARDVQAREMECCLEADLVVCTGRELTNSRRVWNPRTHFVPNGADVELFAKASSPEIP
                     VDPALSGIPGKVLLFLGVMDFRLDVDLLEYVARRAPHWSLVMVGLVKGDVDLSRLTSL
                     PNVHLVDWQPPERLPGFLKSSDVCLIPYVTSQHTNHVFPLKLFEYLAAGKPVVATALK
                     ELAFFEGDLVRLADSPEAFFEAIEIALEADSPQLADCRRLVARHNSWEHRIQALSELI
                     PAASRQSADGPSPAFNGVRAR"
     gene            4794..6167
                     /locus_tag="VNL92_01215"
     CDS             4794..6167
                     /locus_tag="VNL92_01215"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWO93361.1"
                     /translation="MIATERAQRPRPEATRPRQNSLTILALAGSLGIGIACSMALFPV
                     QVAVVLVLAMLVALMALVDIWSTVILLIVARSSLDVFADRAVFGGFNVAGVLAAALIV
                     LGVAHLVLNRVKVSELPLTQPFLALLAVLAFGLSYSQDTLAAVQDWVRMLGSFMLYVL
                     MVSVLTNDTRRASLIRALLLSAIIPLVVGVYQLATGSGDDYTQGFIRIQGTFVHPSPY
                     AFYLLTVLPFAAALAGHARPGWSRLGLILMAGTAAASVLFTFTRIAWLGLVLTLLVLT
                     RAKRWMLLAVLGGVVLVYVFAPAVQDRLSDATSESGSGTWRLEQWQAVLALPSVAQYP
                     TGLGLGAVESLTGIETHNDYLKMLVEGGILGFAALLWLYISLFKHTIAATRLAESRFH
                     QAIATAFLAVLVARAAMCVSDNIIGIPVVEWYFWSLAGLTVALVPRPNDRPTRAPALA
                     GHPGSRP"
     gene            6164..7441
                     /locus_tag="VNL92_01220"
     CDS             6164..7441
                     /locus_tag="VNL92_01220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93362.1"
                     /translation="MRILQVNKFLYPAGGAETVMFQTADLLQRRGHEVFFFGMDDDRN
                     VVAADAGALVSSVNLRERRTKRDLLRPSEWGNAARIVRSAEAARKMERYLDVLRPDVV
                     HLHNIYHQLSPSILPPLRRRGIPTVLTLHDYKLVCPNYMLYAQGAVCERCRGHRYYQA
                     FLQSCVKDSRLQSMLCSFEAYVHQALGGYDRNVDRYVAPSRFMSDKMVAFGMDASRID
                     YLPNFLNVDEFECGQSSEKYFVYAGRVEAVKGVQTLIQAVSEPPISGDVELRIAGDGA
                     LRASLEEHCRIAGRHYVRFLGHLSREEVGRLVRGAMFVVLPSEWYENAPMSILEAYAY
                     GKPVIASRMGGIPEMVDEGVTGLLFEPGNAADLRSRIDLLVGDPALAAEMGRQARKVA
                     EAAYGPDDHYARLMDIYGRAGARSAESEAGRTA"
     gene            7438..8853
                     /locus_tag="VNL92_01225"
     CDS             7438..8853
                     /locus_tag="VNL92_01225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWO93363.1"
                     /translation="MRQLRIAMIGQKGLPATFGGIEHHVEEIGHRLAQRGHRVLVYNR
                     RNYARADKLRYRGMTVRSVPTIHRKHLDAIVHSSLATLHATAFGADVIHYHALGPGVP
                     AILPRYTGRARVVLTVHGLDAERSKWDGAARACLHGAEWLSARVPDATIVVSKELMRH
                     YAHRHHRATWYIPNGVDEPLPSSLSDIEALGLRPGGYILFVGRLVPEKAPDLLIRAFR
                     RVQGDARLVIAGGSCFTDEYVRHLRHLAAADPRVMFPGYVFGAQLSALYANAAAFVLP
                     SMLEGLPLTLLEAASHGTPIVASDISPHVEVLGAEGPGRRLSPAGDEEALALTLAQVL
                     GEQDTERRAARCLREQVLRAYSWDDVVEATESVYRAVLADVREPPPGWQYRRDHIARS
                     DPSGFGDILVGAPTEGAVRLTTSPMLVRETLVQPARLSSADHETAPVHPTPDPELLPN
                     SESIPAGSRVPRVTPSTPGQT"
     gene            9195..>9489
                     /locus_tag="VNL92_01230"
     CDS             9195..>9489
                     /locus_tag="VNL92_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93364.1"
                     /translation="MSDVDVMVYDMFGSPPRALNETQKSRWGMMPWAAHELSPKGVPG
                     DYDWFAGARPGLWTNFAGAITPWGQVYEASTGSPVTDVRIHIRNMELYFLHADG"
BASE COUNT         1598 a         3084 c         2980 g         1827 t
ORIGIN      
        1 ataagttccg ctcgatggtc gatggagccg atggcctaca gatcgccctt gcccgattcg
       61 taggacagga tggccagcca ctcttcaagt tgcctgatga cccgcgcgtg acgcgagtcg
      121 gccgactact gcgccggacg agtctcgacg aacttccgca gctcatcaac gtgcttcgcg
      181 gcgatatgag tctcgttggc ccaaggccgc ccctgcccgg agagatagcc tgcgatagga
      241 ggcacctggc acgactagag gctgtccctg ggatcaccgg gctgtggcag gtgagtggtc
      301 ggagcacgct ctcgtttgag gacatggtcg gcctcgacct ccgttacatc aacggttggt
      361 cgttcacgct cgacctctcg attctgttcc gcacaatccc ggccgttctg agccggaggg
      421 gcgcctgcta gaggcgcctg gagcatcagt cgccgcgcct cgcaagaagg acgcccccat
      481 gccagagcag acccgacttg cactgtacaa atacctcgcc attttggccc gtcgaaaatg
      541 ggtgatcctc acgccagtcg tggtcggggt gctgggggcc gtcgcggcga ccttctggct
      601 gccccaggcc atgccgcgga catacgaagc gacggccacg gcgcaactcg cggtcgcggg
      661 aaggtcggaa aagggtctgt cggacatcaa taaagagcag gccgaccggc tgaccaatac
      721 cttcgtctat gtcctgcagg ggccggacgt cctctccgat gtcatcgcag acctcggcct
      781 cgacgctaca ccgagcgacc tcaagagccg gctgactttc gagccggtcg ccgggacgga
      841 gctcgtcgaa atcaaggcgc gcgctccatc gcccgaggaa gccagggaca ttgccaacgc
      901 catggcgaca gcgtggcgcg agcagggatc ggcgttctac gcaatactgg acacacccga
      961 actgagccgc agctttacgg tggcggagga agcgagcctg cccgcctcgc ccattgagcc
     1021 cgactggcag caaagagtgg gcctcggcct cctcatggga ctagtcgctg gcaccttgct
     1081 ggcgctggcg atcgagtaca cggaccggac catcaactcc ccttcagact tgcgcgatct
     1141 cgtcgaagtc cccgtgctga gtcacttgcc gcgcgttccc aagctacttg gctcgacccg
     1201 acgccgcatc gccgaactcg acgtgcctcc ttatcaatac acgttcgact ccggcgagtt
     1261 gcggctccta acgatcaagc tgctgcacac gctgcaggac cagcacctga acagcctttt
     1321 cgtcacgagc acttggccta gcgaagggac gacgacggtg gccatcggcg tcgccatggg
     1381 actggcgcgc agtggagtcg aagtgctctt gatcgacgcc aatctctcgc gcccggcgct
     1441 gcatacggca tttggcggga tccctgaagc gcccgggctt acggacgtac ttctctcgat
     1501 gggagaggac gagccgccgc aggccctcct caggaagacg atccacgagc cgcctgtcga
     1561 tggactcagc gtactgacgg ccggctcgcc gcacgcggac tcctggaaca tcctcggatc
     1621 gggagagatg aggaaactcg ttgagtcgta cgccggaacc gacactgtcc tcgttatcga
     1681 cggtccgcca gtactgagtt cggtggacgc gttgggcgcg gcggcgatgg cggcgggcgt
     1741 cgtgcttgtc tgcgcggaag gcaaaacatc cgtcgacgac gttcagcgcg gcctcagcca
     1801 gcttgcgctg cttggagcga acgttctcgg cctggtctac aaccaggcgc ggactccgat
     1861 tggcaccatc gctcgttcgg cagccgccgt atgaccagcg tctctcccgc gctcccccat
     1921 ccctccagac ctatcacgga gcgcgcggcg gagcatgcgt cagacgacat gaccctcgcg
     1981 aggcggggga tgagcaacgc tcaggcgcgt gtcgcgcgta acacgctcct acagttggcc
     2041 ggccgtctca tgacgagtct cctgtccctc gccgccgtga gcatcctggc acgttcgcta
     2101 ggcgccgagg aggtcggaag ttatctcctg gtcctctcgc tccttgccat gctcaatctc
     2161 agtgatctcg gcttttcgac catcggcatt cgagagcttg cggctcggcc cgacgagcaa
     2221 gagaagctgc tgggcgagtt catcgtcatc cgcattggcc tggcgctcct tgcgatggtg
     2281 atcgtttcca tcgccgcact cctcttggat ttcggttcag cagtcaccac tgccatctgg
     2341 ctcggctcat taacgtacct gtttaccgcc ctcggccccg ccacgtttgg actcacgttc
     2401 gcggccaacc tccgcatgga gtaccaagtc gcggcgaacg tcattcaggc gggcgtcctc
     2461 gtgggcctgg tggccatgct tgggttgctt ggcggcggca tcgttgccgc cgtgctggct
     2521 tacgacgcgt ccgtgttcgt cggatccatc atgacggtga ttctcgcaag ccgcttcgtc
     2581 cttccccgac tttcgctgga tatccggcgc cattggtggc tcctccgcga atctgcgccg
     2641 atgggattag ccgccctttg ctggcttacg tacaaccgca tcgacatggt gctcatctcg
     2701 aaactcgcgg acgaggaagc gctgggactc tacagtgtcg cgtacaagtc cgttgatctg
     2761 gcctggccca taggcctgag cttcgcgctt tccgcgttcc ccttgttgac acgccacttt
     2821 cgggcgggcg atacggccga gttccagcgc ctcctccaga agtccttcga catgctctcg
     2881 gtgctcatga taccggctgt cacgttcctg attgtatttg cagaacagat cctcgttctc
     2941 gtcggctccg acgacttccg tccagctgcg accagcctgc gactgcttgc gctcgcgatt
     3001 ggcgtgatgc tcgtaagcgt cctgatgtcg cacgctctca tggcggccgg acgacaatcc
     3061 ggcttcttcc gcgcaatgcc catctgggtg tgtatcaaca ttggcctcaa tctcttcctt
     3121 atcccctggc tcgggttcaa cggggcggcc attgccacgt tgttgacgga gctttcgatc
     3181 ctcttgtgtg tactcgtcct gctcgcgcgg cacgtgcacc atgttccgtc gtttgccgtg
     3241 ccggcgagga tgatggcggt ggcgctggcg acggtcgccg ccctcctcgc gctgccagac
     3301 gacccggcgt tccgcatcgt cttcctcccc ttcgccttta tcgtcggcac agcgcttacc
     3361 cgagccgtgg cgatcgccga cctgcgggaa atcgtgcggc gggagcgggt cgcaggagat
     3421 cgaccaggtc tggacgcgag gagccgaccg cccgtttggc ctatgcgagc gccgggtgag
     3481 gagaacgggc gcctgccagg caggctcggc aatgcagcga acggcgcagc gatcaatggc
     3541 tcggagacga cgaaaccgtc ggacccttcg gaccgcgccc tcgaggggcg tgacatcgtg
     3601 tgcctgtcgt tcagtgcgtg ggatgaccat tggggtaccc cccaacaact gatcagccgg
     3661 cttggtaagc ggaatcgcat cctttacgtc gagcccccgg tctcgccgat gtcgttcttt
     3721 acaggcctga ggcgaaagag ctttgtgtgg cagcggttgc gatactcgcg agttggccca
     3781 cgccatgtcg acgctggcat ttgggtcgca gcgccgccaa tggctctgcc gcttcgctac
     3841 acccgaccgg tgagcagggc gaatgcactc atcatgcgcc gctggctcgc gaagcaagtc
     3901 gaggaactgg gcctacgcga tgtgatcttg tggaacttcc agcccctgat ggccggggtc
     3961 gctggcgccg tgaatccggc actgaccatc tatcactgcg tcgatgactt cacatcggtg
     4021 cccaagtggt ggaaccgcgc gcgcgacgtg caggcccggg agatggagtg ctgtcttgaa
     4081 gccgaccttg tcgtctgtac agggcgcgaa ctgaccaaca gcagacgagt gtggaacccc
     4141 cgcacgcact tcgtccccaa tggcgcggac gtggagctct tcgcaaaggc atcgtcgcca
     4201 gagataccag tcgacccggc actaagcgga ataccgggca aggttctcct gttcctcgga
     4261 gttatggact ttcgactgga cgtggacctg ctggagtacg tcgcccgccg cgcgccccat
     4321 tggtcgctcg tgatggtggg cctggtcaag ggcgacgtcg atctctcgcg cctcacctcc
     4381 ctgccgaacg tccacctggt cgattggcag ccgccggagc ggctacccgg cttcctcaag
     4441 tcgagcgacg tttgcctcat accgtatgtc acaagccagc acaccaacca tgtcttcccg
     4501 ttgaagctct tcgagtacct tgccgccgga aagccggtcg tggcgaccgc actgaaggag
     4561 ctcgcgttct tcgaggggga cctcgtgagg ctggcggata gcccggaggc cttctttgag
     4621 gcgatcgaga tcgcactgga ggcggactcc ccgcaattgg cagattgccg tcggctggtg
     4681 gcacgacaca actcctggga gcatcgaatc caggcgctct ccgagctgat tccagccgca
     4741 tctcgccagt cggccgacgg gccgtcgccc gcgttcaacg gggtgcgggc ccgatgatcg
     4801 cgacggaacg agcacaaagg ccccggccgg aagcgacgcg gccacgacag aactcgctta
     4861 cgatcctcgc gctcgccggc agcctcggga tcggcatcgc gtgctcgatg gcgctcttcc
     4921 cggtccaggt ggccgtcgtg ctcgtgctcg cgatgcttgt cgcgctgatg gcgttggtcg
     4981 acatctggag cacggtcatc ctcctcatcg tcgcccgcag cagcttggat gtgttcgccg
     5041 atcgggccgt cttcggcgga ttcaatgttg ccggcgtgct cgcggccgcg ctcatcgtgt
     5101 tgggagtggc gcacctcgta ctgaacaggg tgaaggtgtc ggaactgccg cttacccagc
     5161 cgttcctggc gctcctcgcg gtgctcgcct tcgggctgtc ctactcgcag gacacgctcg
     5221 ctgccgtcca ggattgggtc aggatgctgg gcagcttcat gctgtacgtc ctcatggtca
     5281 gcgttctcac caacgacacg cggcgcgcct ccctcatccg ggcactacta ctgtcagcga
     5341 tcattccgct cgttgtgggg gtatatcagc tggcaacggg ttctggtgac gactacacgc
     5401 aaggcttcat ccgcatccaa ggcaccttcg tgcacccctc tccctatgcc ttctatctcc
     5461 tgacagtgct gcccttcgcg gccgctctcg ccggccatgc gcgccccggg tggagccgcc
     5521 taggcctgat tctcatggcc gggacggccg cggcgagcgt gctgttcacc tttacccgga
     5581 tcgcatggct tggcctggtg ctcaccttgc tcgtgcttac gagggcgaag cggtggatgt
     5641 tgctcgccgt actcggcggt gtcgtcctgg tctacgtgtt cgcaccggcc gtgcaggacc
     5701 gcctgagcga cgcgacaagc gaatcggggt ctggaacgtg gcgactggag cagtggcaag
     5761 ctgtgctggc cctgcccagc gtcgcccaat accccacggg gttgggactt ggtgccgttg
     5821 aatcgctcac gggcatcgag acccacaacg attacctgaa gatgctggtg gaaggcggca
     5881 ttctcggttt cgccgcgttg ctttggctct acataagcct cttcaagcac acgatcgcgg
     5941 cgacgagact tgccgagtcg cgctttcatc aggccattgc gaccgcgttc ttggcggtcc
     6001 tcgtggcgcg cgcggcgatg tgcgtctccg acaacatcat cggcatccct gtggtcgaat
     6061 ggtacttctg gtcactcgcc gggctgaccg tggccctcgt tccccgccca aatgacagac
     6121 cgacccgtgc ccctgctctg gccggtcatc caggaagccg gccatgagga tactccaggt
     6181 caacaagttc ctctatccgg cgggcggggc ggaaaccgtg atgtttcaga cggcagacct
     6241 gctccagcga cgcgggcacg aggtgttctt ctttgggatg gacgatgaca ggaacgtcgt
     6301 ggcggccgac gccggcgcgc tcgtgagcag cgtcaatctc cgggaacgaa ggaccaagcg
     6361 agacctgctg cggccaagcg agtggggcaa cgcggcgcgc atcgtccggt cggcggaggc
     6421 ggcgcgaaag atggagcgct acctcgatgt cctaaggccg gatgtcgttc accttcataa
     6481 catctaccat cagctctcgc cctccatcct gcccccgctg aggcgccgcg gcatccccac
     6541 tgtcctgacg ctgcacgatt acaagctcgt gtgcccgaac tacatgcttt acgcacaagg
     6601 cgcggtctgc gaacggtgcc gcggccaccg gtactaccag gcgttcctcc agagttgtgt
     6661 gaaggactcg cggttgcaga gcatgctctg ctccttcgaa gcgtacgtgc accaagcctt
     6721 ggggggctac gaccgcaacg ttgaccgata cgtcgctccc agtcggttca tgagcgacaa
     6781 gatggtcgcc tttggcatgg atgcgagtcg cattgactac cttccgaact tcctcaatgt
     6841 cgacgagttc gaatgtggcc aatcatccga aaagtacttc gtttatgcag gccgcgtcga
     6901 ggcggtcaag ggagtgcaga cgctgataca ggccgtcagc gagcccccaa tatccgggga
     6961 cgtcgagttg cggattgctg gcgatggagc gctgagggca agtctcgagg agcattgccg
     7021 catcgccgga aggcactacg ttcgcttcct tggccacctc agccgggagg aagtaggccg
     7081 actcgtccga ggggccatgt tcgtcgtatt gccgtccgag tggtacgaga acgcgccaat
     7141 gagcatcttg gaggcttacg cctatggcaa gccggtcatc gcctcgcgga tgggcgggat
     7201 tccggaaatg gtcgacgagg gcgttaccgg cttgcttttc gagccgggca atgcggcaga
     7261 cctgcgctcc aggattgatc tgctcgtcgg cgatccggcg ctggcggctg agatgggcag
     7321 gcaggctcgc aaggtcgccg aggcggcgta cgggcctgac gaccactacg ccaggctcat
     7381 ggacatctac gggagagctg gcgcgaggag tgcggagagc gaggcaggga ggacggcatg
     7441 aggcagctcc gcatcgcgat gatcggccag aagggcctgc cggcgacgtt tggcgggatc
     7501 gagcatcacg tggaggagat cggccatcgg ctggcacagc gggggcaccg ggtcctcgtg
     7561 tacaaccggc gcaactatgc tcgagccgac aagctccggt accgcgggat gacggtccga
     7621 tcggtgccaa ccatacaccg aaagcatctg gacgcgatcg tacacagcag cctcgcgaca
     7681 cttcacgcca cagcattcgg tgccgacgtc attcactatc acgccttggg gccaggagtt
     7741 cccgccatcc tgccgcgcta tacaggtcgt gcgcgggtgg tcctgaccgt acatggcttg
     7801 gatgccgagc gatcgaagtg ggacggggcg gcccgagcct gcctgcacgg tgccgagtgg
     7861 ctctccgccc gcgtgccgga cgccaccatt gttgtctcga aagagctcat gcgccactac
     7921 gcccaccggc atcatcgcgc gacgtggtac atcccaaacg gcgttgacga gccgctcccg
     7981 tcgtcgttaa gcgacataga agcgttagga ctgcgcccag gagggtacat cctcttcgtg
     8041 ggtcggctgg tgccggagaa agctccggat ctcctgatac gcgcgttccg ccgtgtacag
     8101 ggcgacgccc gcctcgtcat cgcgggaggc tcctgtttca ccgacgagta cgtgcgccat
     8161 ttgcggcacc ttgctgccgc cgacccgcgg gtgatgttcc ccggctatgt cttcggggct
     8221 cagctgtcgg cgctctacgc caacgctgcg gccttcgtgc tcccctcgat gcttgagggc
     8281 ctgccactga ccctgctgga agcggcctcc cacggaacac ccatagtcgc cagcgacatt
     8341 tccccccacg tggaggtgct cggcgccgag ggcccgggcc ggcggctctc gcccgccgga
     8401 gatgaggagg cgctcgccct gacactcgcc caggtgttgg gggagcagga caccgagcgt
     8461 cgggctgcgc gctgtctgcg agaacaagtg ctccgcgcct actcgtggga cgatgtggtc
     8521 gaggccacgg agagcgtcta ccgagccgtg cttgcggacg tacgcgaacc gccgccggga
     8581 tggcagtatc gccgggatca tattgcgcga tccgatccga gtggcttcgg agatatcctc
     8641 gtcggggcgc ccacggaagg ggcggtgcga ctgacgacct cccctatgct cgtacgcgaa
     8701 acgttggtac agcctgcgag actgtcctcc gctgaccacg aaacggcacc cgtgcatcct
     8761 acccctgacc cggagctcct accgaactcc gaatccatac ccgcagggtc ccgcgtgcca
     8821 agggtaacac cgtcgacgcc gggccaaacg tgaagcggtg gccgaacgtg gggcgccccc
     8881 ctcgccgtcg ccgcggtgtg ggggtaccgc gctgaattcg cgccgatctt cccatgccac
     8941 aagttgcctc cccggcgaat cggccgaccg atgctatgat agattgatat ggtgtggttc
     9001 aacgcgatcg tgaccaagag caacctcggc ggtcacgtca ctgatgagcc cgaaccggtg
     9061 gtaccctcgc ggtgaggcca agcatagcag ctcgaattct ccgtttagcg gcagccctct
     9121 ccatcgctct ggtcctcgcg accatgcccg gggcgtcttg gtctgatgcc actcccactg
     9181 aagcccaggg ccccatgtcc gacgtcgacg taatggtgta cgacatgttc ggctcgccgc
     9241 cgcgagctct taacgaaaca cagaagagcc gctggggaat gatgccctgg gccgcacatg
     9301 agctttcgcc gaagggcgtt ccgggcgact acgactggtt tgccggtgcc aggccgggtc
     9361 tgtggacaaa cttcgcaggg gcgattaccc cctgggggca agtctacgag gcttcaacgg
     9421 gaagccccgt cactgatgtc cgcatccaca tccgcaacat ggagctgtac ttccttcacg
     9481 ctgatgggg
//
LOCUS       DATMBB010000060         7888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_68122, whole genome shotgun sequence.
ACCESSION   DATMBB010000060 DATMBB010000000
VERSION     DATMBB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68122"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VNL92_01235"
     CDS             <1..635
                     /locus_tag="VNL92_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HWO93365.1"
                     /translation="TRFLPAEDRHLGTRRQFRAMLASMLGGKTAEELVFGEMTTGASD
                     DISRATKLARQMVTQYGMSERLGPRTFGRKEELVFLGREISEQRDYSEKVAEEIDEEV
                     ASIIDEAHQTARRALEENRGKLDRIAEVLIEVETIEGEPLQRLFRGEDPFPGKARDEQ
                     PKAPEEPEEQEEAGSEGRDRPKPAAQPGLAWGGGPATIRLEKPDDAVGPN"
     gene            793..2460
                     /gene="metG"
                     /locus_tag="VNL92_01240"
     CDS             793..2460
                     /gene="metG"
                     /locus_tag="VNL92_01240"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWO93366.1"
                     /translation="MSERIAIGVAWPYANGSLHLGQIAGAYLPADIFARYHRAAGSDV
                     LMVSGSDQHGTPITVRAESEGVSPQQIVDRFHAEFLETWPRLGISFDLFTATGTENHR
                     RVVQDVFLRLYEKGDIYKATMTLPYCPVDQRFLLDRYVEGTCPHCGFAEARGDQCDNC
                     GRPLDPLQLHGPRCKFCGTTPEPRESEHFFMRLSAFTERIREWVTPFGEHWRRNVYNF
                     TLGILKEGLHDRAITRDIEWGVPIPLPGYEKKRIYVWFEAFIGYLSASMEWAQRAGEP
                     EAWRPFWQDPATKSYYFIGKDNIFFHTIWWPAVLMAHGDLILPYDVPANQYLTMSGSK
                     ASTSRNWAVWAPDALARYDADAIRYTLAALMPETSDSDFTWAEFLRRNNDELVATWGN
                     LANRVLTFTVRNFDGRVPERGQLDSVSVALLQNAKTALGEIGDEIAACRFRSGLAKAM
                     ALAQDANRYLDERAPWKTVQDDRESAGQALYTAIAVIGTLRTAFSPYLPFSSDRLRSQ
                     LGLPDDGASTKWCYVDPVPGTRLGTPEPLFRKLDPSMVEEEEARMGR"
     gene            2460..3296
                     /locus_tag="VNL92_01245"
     CDS             2460..3296
                     /locus_tag="VNL92_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWO93367.1"
                     /translation="MLPLIDTHAHPQFSAFDDDRDIVLERARAAGLQALIAVGTDIPT
                     SEAALDLAERHDWVYATAGIHPHDAEKATHSDPYLIRTYASHPRAVAVGETGLDYYRM
                     LSSREAQIALFRAHLVIAGGIGRPVVVHSRSAADDTWDILCEWAAGLDRDGREQRPIG
                     VMHCFEGDLAQAERYVSIGFLISIPGPVTYPNAARRQEVAAGLALDAMVVETDCPYLT
                     PQRYRGHRNEPAYLVETVQTIASLRSQSPAEIAQATSANASRLFGIPTPSGVTPDRGS
                     NR"
     gene            3293..4270
                     /locus_tag="VNL92_01250"
     CDS             3293..4270
                     /locus_tag="VNL92_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWO93368.1"
                     /translation="MTTTTAALYGPVQEDMSLVEERLIEVKQVDHPWLAQMLEAALAA
                     GGKRLRPAVALLSGKFHTYDLPRLATLAAALELLHTATLVHDDVIDNASTRRGRPTVN
                     AQFKNAVTVMLGDYMFANAAVLISQTEHVKVIENFSRSLMMIATGELGQDLSTFDTRQ
                     DIRSYLQRIGGKTAALFRTAGECGALLSNAPSQHVGALRDYGYHLGMAFQIVDDILDF
                     TGDEADLGKPVGSDLMQGTLTLPALIFLEREPANNPITKLFRARRNRQSELLIKAVHG
                     VLESGAIEEARRVAEDFAARARDALAPLPLHPARQTLAELTYYVLNRKS"
     gene            complement(4282..6027)
                     /locus_tag="VNL92_01255"
     CDS             complement(4282..6027)
                     /locus_tag="VNL92_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWO93369.1"
                     /translation="MGMQTSYPEPGLRFGPSRRRVASVLFALLLLTLGTGYGAFAMVT
                     QVDQLLFPGNEIRVNLIGNAPGLDVKPPDALAANEDRINLLLIGLDRRPGEQAEKDAY
                     RSDTIVVLSIHPEQKKASMISFPRDLWVQIPEGDGEVSEGRINEAYYRGQYYDYEGGG
                     PGLVADTLTHNFGIAVDRYVVLEFEDFVELIDALGGIDIEIPDHVAYLYSTDEVPGHE
                     RWFEVGPGRHHFSGDDALAYARYREDSDLYRIQRQQRVIFAAIDRALSLGMLSRAPEL
                     WDKYQDAVDTDISAFQVPGYAALLKGISSDQIASYSLGDAVLGFTTAGGASVLRLLPD
                     QAAPILGRAFRPPEVDQENARIALLNATGEPGLAHEFELYLQIQGIEPANMTFGNAPE
                     GWPMGEAAVLYVPGAKNTARFLGEWIEIDDVREATPEEVQLLFGDAQVVAILGDGVQP
                     RLVALPGTENGAAPASSFIGAEAPPQYDPYEPPVEYFEEPDVVILEEEPVAPLPDETE
                     PPVDEGVVEPPVDPGVGTGEGTVEPPPVTEEPPPVEEPPVDPPPPPPDEENPFGGEDD
                     NPFATVPEAADPTLG"
     gene            6083..6943
                     /locus_tag="VNL92_01260"
     CDS             6083..6943
                     /locus_tag="VNL92_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWO93370.1"
                     /translation="MIVDAHTHVFPPSIAASRDAYLRRDATFAALYSTPKSRIATPEQ
                     LISSMDRSGIDVSIALGFGWSSNQLCTEGNETVMAAAAANPRLIAFATVWPGEPDVAY
                     REAERCARGGCRGLGELRPRGSGSGPSYSLSGEAGDALASAARDFDLTLLFHVSEPVG
                     HRYPGKEGLEIAELAEFCARYPDVRVIAAHWGGGLPFYLLMPEMRTALANVWFDTAAT
                     SLLYRDDVFRVAASLSAVDRVLFGSDFPLLDQARQRKLIENAPGLSDDDRLAILGENA
                     ARLFGLEAMR"
     gene            6940..7524
                     /gene="thpR"
                     /locus_tag="VNL92_01265"
     CDS             6940..7524
                     /gene="thpR"
                     /locus_tag="VNL92_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWO93371.1"
                     /translation="MTPKPIRLFVAIELPDDVMAAIGELEDELSREAPHVRWARPDRV
                     HLTLKFIGETPPNRLPEIEAALAGLPPWHALDLRTGPLGYFGRPSALRTIWLGVEGAL
                     DELRQLQAAVDEALVAAGVVNADDRPYTPHLTLARVPNGIAPDEHARMAALIKRTQVA
                     PHAFEGHTITLFRSNLGPQRPRYEALRRFSAFAS"
     gene            7614..>7888
                     /locus_tag="VNL92_01270"
     CDS             7614..>7888
                     /locus_tag="VNL92_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93372.1"
                     /translation="MEQPRVIAERIAENVERVIVGKSREVRLALVALLSQGHLLLEDV
                     PGVGKTVLARSLARSTGCSFRRIQFTPDLLPTDVTGVSIFDQRDRDF"
BASE COUNT         1470 a         2641 c         2454 g         1323 t
ORIGIN      
        1 acacccgctt cttgccggcc gaagaccgcc atctcggcac acggcgtcaa ttccgggcaa
       61 tgctcgcttc gatgctcggc ggcaagacgg cggaagagct cgtcttcggt gagatgacga
      121 ccggcgcctc cgatgacatc agccgggcca ccaagctcgc gcgtcagatg gtaacgcaat
      181 acggcatgag cgagcgcctt ggcccacgca ctttcggacg caaggaagag ctcgtcttcc
      241 tcggccgcga gatctccgag cagcgcgact attccgaaaa ggtcgctgag gaaatcgacg
      301 aggaagtcgc gagcatcatc gacgaagcgc accaaacggc gcgacgagcg cttgaggaaa
      361 accgcggaaa gctcgaccgc atcgccgagg tcctcatcga ggtggagacg atcgagggcg
      421 agccgctcca acgtctcttc cggggagagg atccgttccc cggcaaagcg cgcgacgagc
      481 agccgaaggc gccggaggag ccggaggagc aagaagaggc gggcagcgaa gggcgcgacc
      541 ggccgaagcc ggcggctcag ccgggcctcg cgtggggtgg cggtcctgca accatccgac
      601 tggagaagcc cgacgacgct gtcgggccga actaggacct tctcgtaatc ctggtgaggg
      661 gcctcggtat ctctatgccg aggcccctcg gtatgctctg cgattcgccc ggaacgattg
      721 ggaaccaaag cacaagggct gtgctacaat gacgcccacc gcattccatc gcccgaccga
      781 ggcgcacaac acgtgtccga acggattgct atcggagtcg cttggcccta tgcaaatggc
      841 tcgcttcacc tcggccaaat agctggggca tacttaccgg ccgatatctt cgcgcgctac
      901 catcgagccg cgggcagcga cgtgctgatg gtctcgggca gcgaccagca tggcacgccc
      961 atcacggtcc gggcggagag cgaaggcgtc agcccccagc aaatcgtcga tcgctttcac
     1021 gccgaattcc tcgaaacgtg gccgcgcctc ggcatctcct tcgatctgtt cacggcaacg
     1081 ggtacggaga accatcggcg cgtggtccaa gatgtgtttc ttcggctcta cgaaaagggc
     1141 gacatctata aggccaccat gacgttgccg tattgccccg tcgaccagcg cttcctcctg
     1201 gaccgctacg tcgagggcac gtgcccgcat tgtggattcg ctgaggcccg cggagaccag
     1261 tgcgacaatt gcggtcgtcc gctcgacccg ctgcaactcc acggccctcg ctgcaagttc
     1321 tgtggaacca caccggaacc ccgcgagtcg gagcacttct tcatgcggct ctccgcgttc
     1381 accgaacgca ttcgtgaatg ggtcacgccg ttcggggagc actggcgaag gaacgtctac
     1441 aactttaccc tcggcatcct caaagagggt ctgcacgata gggccatcac ccgcgatatc
     1501 gagtgggggg tgcccatccc gctacctgga tatgagaaga agcgcatcta cgtgtggttc
     1561 gaggcgttca tcggctacct gtcggcatcg atggagtggg cgcagcgcgc cggagagcct
     1621 gaagcatggc gtccattctg gcaagaccca gcgacgaagt cctactactt cattggcaag
     1681 gacaacatct tcttccacac catctggtgg cccgccgtcc tgatggccca cggcgacctc
     1741 atcttgccgt atgacgtgcc cgcgaaccag tacctcacga tgtccggtag caaggcatcg
     1801 acgagccgca actgggccgt gtgggcgccg gatgccctcg cccgatacga cgcggacgcg
     1861 attcgctaca cgctggccgc cctcatgccc gaaacgagcg actccgactt cacgtgggcc
     1921 gagttcctcc gtcgcaacaa tgacgagctc gtcgctacct ggggcaacct cgcgaaccgt
     1981 gtgctgacct tcaccgttcg caacttcgat gggcgcgtgc cggagcgcgg ccagctggac
     2041 tccgtgagcg tcgcactcct tcagaacgcc aagacggccc tcggcgagat cggcgacgag
     2101 atcgccgcct gtcgcttccg cagcggtctc gcaaaagcga tggcgctggc ccaagacgcc
     2161 aaccgctacc tcgacgaacg ggctccgtgg aagacggtcc aggacgaccg cgagtctgct
     2221 ggccaggcgc tctacacagc aatcgccgtt atcggcacgc tgagaacagc cttctccccg
     2281 tacctgccgt tttccagcga tcggttgcgt agccagctcg ggcttccgga cgatggcgca
     2341 tccaccaagt ggtgctacgt cgatccggtt cccggaacac gcctcggtac gccagaacca
     2401 ttgttccgca aactcgatcc atcgatggtg gaggaagagg aagcgcggat gggccggtga
     2461 tgcttccatt gatcgatacg cacgcgcacc cgcaattcag cgcgttcgac gacgaccgcg
     2521 acattgtcct cgaacgtgcg cgagccgccg gcttgcaggc actcatcgcc gtcgggaccg
     2581 acatcccgac cagcgaggca gcgctcgacc tcgcggagag acacgattgg gtctacgcta
     2641 ccgccggcat ccacccccat gatgcggaga aggcaacgca cagcgatccg tatctcatcc
     2701 gcacgtacgc gtcgcatccc cgcgccgtcg cagtagggga gaccggcctc gactactatc
     2761 gaatgctatc atcgcgcgaa gcgcagatcg cgttgttccg cgcccatctc gtcatcgccg
     2821 gcggcatcgg acggcctgtg gtcgtccatt cacgctccgc agcggacgat acgtgggata
     2881 ttctctgcga gtgggcggcc ggcctcgatc gggatgggcg ggaacagcga cccatcggcg
     2941 tcatgcactg cttcgaggga gacctcgctc aggcggagcg ctacgtgagc atcggcttcc
     3001 tcatctcgat ccctggtccc gtcacgtacc caaacgcggc ccgcaggcag gaggtagccg
     3061 cgggccttgc actcgacgcg atggtggtcg agaccgactg tccctacctg acaccccaac
     3121 ggtaccgcgg tcatcgcaat gagcccgcct atctcgttga gaccgtgcaa accatcgcgt
     3181 cgcttcggag tcaatcgcct gcggagatcg cgcaggcgac gagcgcaaac gcatcccgcc
     3241 tattcgggat accgacgccg agcggcgtga caccagacag agggagtaac cggtgaccac
     3301 gacgacagcc gcactctatg gtcccgtcca ggaggacatg tcgctcgtgg aggagcgcct
     3361 gatcgaggtc aaacaggtcg atcacccgtg gctggcgcag atgctcgagg cagcattggc
     3421 ggccgggggg aagcgattga ggcccgccgt cgccctcctc agcggcaagt tccacacgta
     3481 cgatctgccc cgccttgcga cgctcgctgc ggcactcgag ctgctccaca cggcgaccct
     3541 ggtgcacgat gacgtgatcg acaacgcgag cacgagacgc ggacggccca cggtcaacgc
     3601 ccagttcaag aacgcggtca ccgtcatgct tggtgattac atgttcgcga atgcggcggt
     3661 cctcatctcc caaacggagc acgtcaaggt catcgagaat ttctcgcgct ccctcatgat
     3721 gatcgcgacc ggggagctcg gacaggatct ctcgactttc gacacgcgcc aagacatacg
     3781 ttcgtacttg caacgcatcg gcggcaagac agccgccctg ttccgtaccg ccggtgaatg
     3841 cggtgccttg ttgtcgaacg cgccgtcgca acacgtcggc gcactccggg actacggata
     3901 ccaccttggg atggccttcc agatcgtcga tgacatcctc gactttaccg gcgacgaggc
     3961 cgacctcggg aagcccgtcg gcagcgacct gatgcaagga acgctcacgc tgccggccct
     4021 catcttcctc gagcgcgaac cggccaacaa cccgatcacg aagctgttcc gagcgcgacg
     4081 caacaggcag agcgaactgc tgatcaaggc agtgcacggc gtgctcgaaa gcggcgcgat
     4141 cgaggaggcg cgccgcgtgg cggaggactt cgcagcgcgc gcacgcgatg ccctcgctcc
     4201 attgccgctc cacccagcga gacaaaccct ggcggagctt acgtactacg tcctgaaccg
     4261 aaagagctga ctcgccgagc gctaccccaa agtcgggtca gcggcctccg gaaccgtggc
     4321 gaacggattg tcgtcctcgc ccccaaaggg gttctcctcg tcgggcggcg gtgggggagg
     4381 atcgacgggc ggctcctcga cgggcggagg ctcctccgtc acgggcgggg gttcaaccgt
     4441 tccctcaccg gtgcccacgc ctggatcgac cggcggctcg acgacaccct catcgacggg
     4501 cggttccgtc tcgtccggca ggggcgcgac cggctcctcc tcgaggatga cgacgtccgg
     4561 ctcctcgaag tactcgacgg gtggttcgta gggatcgtac tgcggcggcg cctcggcacc
     4621 gatgaacgac gaggcggggg cggcgccgtt ttcggtgccg ggcaaggcaa cgagtcgcgg
     4681 ttggacacca tcgccgagga tagccacgac ctgcgcatcg ccgaacagga gctgcacttc
     4741 ctccggagtg gcctctcgaa cgtcgtcgat ctcgatccat tcccccagga agcgagccgt
     4801 gttcttggcc cccggaacgt acaacacggc ggcttcgccc attggccatc cctcaggagc
     4861 gttgccaaac gtcatgttgg cgggctcgat gccctgaatc tggagataca actcgaactc
     4921 gtgggccaag ccgggctcgc ccgtcgcgtt gagcagggcg atacgcgcgt tctcctgatc
     4981 gacctctggc ggacggaacg cgcggcccag aatcggcgcc gcctggtccg gcaacaagcg
     5041 caggaccgag gcgcccccgg ccgtcgtgaa gccgagcacc gcatcgccga gggagtacga
     5101 ggcgatctgg tcggacgaaa tgcccttgag gagcgccgca tagcccggca cctggaacgc
     5161 gctgatatcc gtgtcgactg catcctggta cttgtcccaa agctcgggcg ctcgactcag
     5221 catgccgagc gagagggcac ggtcgatggc agcgaagatc acgcgctgct gccgctggat
     5281 ccggtagagg tcggagtcct cgcggtagcg tgcgtacgcc agcgcatcgt cgcccgagaa
     5341 gtgatgccgg cccgggccga cctcgaacca tcgctcgtgg ccggggactt cgtcagtgga
     5401 gtagaggtag gcaacgtggt ccggaatctc gatatcgatg ccacccagcg catcgatgag
     5461 ctccacaaag tcctcgaact ccaggacgac ataccgatcg accgcgatgc cgaagttgtg
     5521 ggtcagagtg tcagctacta agccggggcc gcccccctcg tagtcgtaat actgccctcg
     5581 gtagtatgcc tcgttgatgc gcccctcgct gacttcgccg tcgccctctg gaatctgaac
     5641 ccagaggtcg cgcgggaagc taatcatcga ggccttcttt tgctcaggat ggatcgaaag
     5701 cacgacgatc gtatccgaac gataggcatc cttctccgcc tgctcgccgg gcctcctatc
     5761 gaggccgatc aggaggagat tgatacgatc ctcgttcgcg gctagggcgt cgggtggttt
     5821 gacatcgagg cccggcgcgt tgcctatgag attgacccga atctcattgc cgggaaacaa
     5881 tagctggtcg acctgtgtga ccatggcaaa cgcgccgtag cccgtcccca gggtcagtag
     5941 cagcaaggcg aacagcacgg aggccacgcg ccgccggctc ggcccgaagc gcaggccggg
     6001 ctcggggtaa ctggtttgca tgcccatcga gaaattatag gaatcgcccg tccgtaggcg
     6061 caaacgcagg gccagcgaag acgtgatcgt cgatgctcat acccacgtat tccctccatc
     6121 aatcgccgcc agccgcgatg catatctccg gcgcgatgca accttcgccg cgctttactc
     6181 cacgccaaag tcacggatcg caacgccaga gcagctcatc tccagcatgg accggtctgg
     6241 gatcgacgtg agcatcgcgc tgggattcgg ttggtccagc aaccagctct gcacggaagg
     6301 aaacgagacc gtcatggcgg cggcagcggc gaatccgcgg ctcatcgcat tcgcgacggt
     6361 atggccaggg gaaccggatg tggcctaccg cgaggcggaa cgctgcgcgc gaggggggtg
     6421 ccgcggcctc ggcgagctgc gcccccgcgg ctccggaagt ggcccgagct actcgctttc
     6481 aggggaagcc ggcgatgccc tcgcctcggc agcgcgcgat ttcgacctga ccctgctctt
     6541 ccacgtgagc gaaccggtgg gccaccgcta tcccggcaag gaagggctcg agatcgccga
     6601 actcgccgag ttctgcgcgc gctatcccga tgtccgggtc atcgccgctc actggggagg
     6661 cggccttccg ttctacctgc tcatgcccga gatgcgaacc gctctcgcca acgtctggtt
     6721 cgataccgcg gcgacgagct tgctctacag ggacgacgtc tttcgcgtcg ctgcctcact
     6781 ctcggccgtc gaccgcgtgc tgttcggcag cgatttcccg ttgctcgacc aggcgcggca
     6841 gcgcaagttg atcgaaaacg caccgggtct ctccgatgac gatcgactcg cgatactcgg
     6901 cgagaacgcg gcgcggctat tcggcctcga ggcgatgcgt tgacgcccaa gcccatccgc
     6961 ctgttcgtcg ccatcgagct cccggacgac gtgatggccg ccatcggcga gctggaagac
     7021 gagctcagcc gcgaagcgcc gcatgtccgt tgggcacgac cggatcgcgt gcacctcacc
     7081 ctcaagttca tcggcgaaac tccgccgaac cgccttccgg agatcgaggc cgcactggcc
     7141 gggctgcccc cctggcacgc gctcgatctc cgaacgggtc ccttgggata cttcggacgt
     7201 ccgagcgccc tccgaaccat ctggcttggg gtcgaagggg cgcttgacga gttgcgccaa
     7261 ctgcaggcgg ccgtcgacga agcgctcgtc gcagcgggag tcgtgaacgc ggacgatcgt
     7321 ccctacacac ctcacctcac actcgcgcgc gtgcccaacg gcatcgctcc ggacgagcat
     7381 gctcgcatgg cggcgctcat caagcgcacc caggtcgcgc cccatgcgtt cgaaggccat
     7441 accatcacgc tgttccgcag caaccttggc ccacagagac cgcgctacga agcactgcgc
     7501 cgcttttcgg ccttcgcgtc ctgattctcg cgccacccgt ccggctgtat actcgaatct
     7561 gacccgctcg ttacgcgcgt ttcctaaggg aacgtcatat tgggagggaa gccgtggaac
     7621 agcctcgcgt catcgccgag cgcatcgcgg agaatgtgga gcgcgtcatc gtcgggaaga
     7681 gccgcgaggt ccggcttgcc ctcgtggccc tgctctccca gggccacctc ctgctggaag
     7741 acgtaccggg tgtggggaag acggtgttgg cgcgcagcct cgcgcgatcg accgggtgct
     7801 cgttccgtcg tatccagttc acgccggacc ttctacccac cgacgtgacg ggcgtatcca
     7861 tattcgacca gcgcgaccga gatttcga
//
LOCUS       DATMBB010000061         3825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_20347, whole genome shotgun sequence.
ACCESSION   DATMBB010000061 DATMBB010000000
VERSION     DATMBB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20347"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VNL92_01275"
     CDS             complement(<1..559)
                     /locus_tag="VNL92_01275"
                     /inference="COORDINATES: protein motif:HMM:NF022235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     acid halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="halocarboxylic acid dehydrogenase DehI family
                     protein"
                     /protein_id="HWO93373.1"
                     /translation="MAVNIALPLNEVDAAPEARAVFAEAREALRTPFTPGLIRSLAQW
                     PAYLAYVWEQLRPSIETAGFRGSAQYLAAMAREAVDQFYEATYARNTLLDSGLSEFDI
                     ETIAGGLEVLHLTNPQCLLIAGALTEALERPRVGGMGRPEPRDISEEERRILAYEWRM
                     LDEGEMSADMRRLAADVREVLGLPFL"
     gene            648..1901
                     /locus_tag="VNL92_01280"
     CDS             648..1901
                     /locus_tag="VNL92_01280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HWO93374.1"
                     /translation="MAILDTAIDGPPAQEKRARRRKRDRRPVPREFIRLRITDMAHRG
                     YALGRYDGQVVFAAHGIPGEDVTVEITQRRQDYLVGRVVEVHEASPARVDAPCQYYGR
                     CGGCQLQHIDYAEQLRLKTHIVREQLRRFAHLSDTPVLPAVPCDPPWAYRNQTRFTVW
                     HGALGFVGAQTHRFVRIDECLIADPAINETLQQLQDRVPGARQVMVRVGVNTGDRLLQ
                     PSLERFPDVTLPSGQRHLHESLLGADFRVSASSFFQVNTRQAETLVRLTIDALDLQRS
                     DVVLDAYAGVGTFAKIVAPQVCQVIAIEEAASAVDDAKVNLAGIANVRIELGAVENLL
                     PTLGVTPDAAILDPPRTGCHRSTIEAIVGGGIERLVYVSCEPATLARDIALLIEGGYE
                     LRSVQPVDMFPQTYHIECVAGLRRV"
     gene            complement(1911..2942)
                     /locus_tag="VNL92_01285"
     CDS             complement(1911..2942)
                     /locus_tag="VNL92_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWO93375.1"
                     /translation="MTIGQASGVNAGQRRGLALRLLPWALRLVVTAGLAALIVLRVDL
                     SEMAQAWQGFDLRWLTPALLVTASVRLLDAVRWRLYLSKYGEAPFSGLLGAFLIGNLG
                     NTLLPFRAGDLAKVQILANRYRIPRAALTSSTFILESVTDGVTFSLLLLASLPFLDVP
                     AVPDALLLAFASLGLGGFVIVTLLSRTLPADLFDQAWPRRLIATDLREWLHRQWPAFL
                     DGLETLRRRRLWGPVLVLNTVGWTVQAISFWMFGLAFDLDLSFSNYLVVMVTANLVGA
                     IPITFFNFGTYEAAVLELVAAWGVARDEALAFALATHLLTNLLIIALGIAAAAVMRVQ
                     IREIFRLRD"
     gene            3011..3646
                     /locus_tag="VNL92_01290"
     CDS             3011..3646
                     /locus_tag="VNL92_01290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWO93376.1"
                     /translation="MVEPHLVLASASPRRRALLAELGLPFSVRAVDIDEEHALATAPS
                     QSPLDLAMRVAAAKAEASVRHANEVVLTADTLVMLDGRPLGKPRDACEAKQMLEALRG
                     REHVVVTGVAVVAAAERFVEATEATVTMREYAADEISRYIERGEPFDKAGAYAIQDTV
                     FAPVASYRGCYCTIVGLPLWCTRRLLRSVGLRPEAPTRAVCRACPDREEGG"
BASE COUNT          707 a         1250 c         1235 g          633 t
ORIGIN      
        1 tgaggaacgg caggccgagc acctcgcgca catcggcagc caaccgccgc atatcggccg
       61 acatctcgcc ctcgtcgagc atcctccatt cgtaagcgag gatgcggcgt tcttcctcgc
      121 tgatatcgcg aggctccggc cgacccatgc cgcccacgcg aggacgctcc agcgcttcgg
      181 tgagcgcgcc cgcgatgagg aggcactgcg ggttcgtcag gtgcaggact tcgagcccgc
      241 ccgcaatagt ctcgatatcg aactcggaca ggcccgaatc caggagcgtg ttgcgcgcgt
      301 acgtcgcctc gtagaactgg tcgacggcct cacgggccat cgcggccaga tactgggcgc
      361 tgccgcggaa cccggccgtc tcgatactcg ggcgcagctg ctcccaaaca taggcaaggt
      421 acgcgggcca ttgcgcgagt gatcgaatca gcccgggcgt gaatggagtg cgcagcgcct
      481 cgcgcgcctc ggcgaacaca gctcgcgcct ctggagccgc gtccacctcg ttgagtggaa
      541 gcgcgatgtt gactgccatg cactgattct agcattcgtt tgagtgaggc ttcgccccac
      601 ccccgtggca ccttgttcgc ggatgggaca gccccgacaa tggagccatg gccatcctcg
      661 acacagcgat cgatgggccg cccgcgcaag aaaagcgcgc gcggcggcgg aagcgcgata
      721 ggcggcccgt tccacgcgag ttcatccggc tccgcatcac cgatatggcg catcgtggct
      781 atgctctcgg ccggtacgat gggcaagtcg tgttcgccgc tcacggcatc cccggcgagg
      841 acgtgaccgt cgagatcact caacggcggc aggactatct ggtggggcgc gtggtcgagg
      901 tgcacgaagc gtcgccggcg cgcgtcgacg caccatgcca gtactacggc cggtgtggcg
      961 gctgtcagtt gcagcacatc gactacgcgg aacagctccg gctgaagacg cacatcgtgc
     1021 gagagcagct acgccgcttt gcgcacctct cggacacgcc ggtcctgccg gcggtcccgt
     1081 gcgatcctcc gtgggcgtat cgaaaccaga cccgcttcac cgtttggcat ggagctttgg
     1141 gcttcgtcgg cgcccagacg catcgattcg tccgcatcga cgagtgtctc atcgccgacc
     1201 cggccatcaa cgagacgctg cagcagttgc aagatcgcgt acccggcgct cgtcaggtga
     1261 tggtacgcgt cggcgtcaac accggcgatc gcttgctgca gccgtcgttg gagcgcttcc
     1321 ccgacgtgac gttgcccagc gggcaacgcc atcttcacga gtcgcttctt ggcgccgact
     1381 ttcgggtctc ggcatcatcg ttctttcaag tgaacacgcg ccaggcggaa accctcgttc
     1441 ggctgaccat cgatgctctt gacctgcagc gaagtgacgt agttctcgat gcatacgccg
     1501 gcgtgggtac cttcgcgaag atcgtcgcgc cccaggtctg tcaggtaatc gcgatcgagg
     1561 aagccgcgtc tgcagttgac gatgcgaagg tgaacctcgc tggcatcgcc aacgtccgta
     1621 tcgagctcgg cgccgtcgag aacctactcc cgacgctcgg ggtgacgccg gacgccgcta
     1681 tcctcgatcc gccgcggacc ggctgtcacc ggagcacgat tgaggcgatc gtcggtggcg
     1741 gcattgagcg gctcgtatac gtgtcctgcg agccggccac cctcgcccgg gatatcgcgc
     1801 tcctcatcga ggggggatac gagttgcgat ctgtccagcc cgtggacatg tttccccaga
     1861 catatcacat cgagtgcgtc gcggggcttc gccgtgtcta agtagctcgc tcaatctctt
     1921 agtctgaaga tttctcgaat ctggacgcgc atgaccgccg cggcggcgat ccccagggcg
     1981 atgatgagga ggttcgtcag gagatgcgtg gccagtgcaa acgcgagcgc ctcgtcgcgc
     2041 gccacacccc aggcggcaac gagctccagg acggccgctt cgtaggtacc gaagttgaag
     2101 aacgtgatag gtatggcgcc cacgagattt gccgtcacca tcaccacaag gtaattggag
     2161 aacgacaagt cgagatcgaa ggccaggcca aacatccaga acgagatggc ctgtaccgtc
     2221 cacccgacgg tgttgagtac gagaaccggc ccccagagcc ggcggcgccg caacgtctcc
     2281 aagccatcga gaaacgccgg ccactgacgg tggagccact cgcgcagatc cgtcgcgatg
     2341 aggcggcggg gccaagcctg gtcgaacaag tcggccggca gtgtccgcga gagaagggtc
     2401 acgatgacga acccgcccaa tcccaaagac gcgaatgcca agaggagggc atcggggacg
     2461 gcgggaacat cgaggaacgg caggctagcg agcagcagca gcgagaacgt caccccatcc
     2521 gtaacgctct cgaggatgaa ggtcgacgaa gtgagcgccg cacgcgggat gcgatagcga
     2581 ttcgcaagaa tctgaacctt cgcaaggtcg ccggcgcgaa atggaaggag cgtgtttcct
     2641 agattgccaa tgaggaaggc acccaggagg ccggagaatg gcgcttctcc gtacttggag
     2701 aggtataacc gccagcgtac cgcgtcgagc agccgaacgc tcgccgtgac aagcagtgcc
     2761 ggtgtcagcc atcgcaggtc gaatccctgc cacgcttgcg ccatctcgct gaggtcaacg
     2821 cgcagcacga tgagtgcagc caggcccgcg gtgacgacta gcctcagggc ccacggcagc
     2881 aagcgaagcg ccaggccccg ccgttggccg gcattcacac ccgaggcttg gcctatggtc
     2941 atgggccgga tggtggggtt tcttcatgcc ggccgtcaag ccatgagggc agcaaccacc
     3001 ggagcgagtc gtggttgagc cgcaccttgt cctcgcctcg gcgtcgccga ggcgccgtgc
     3061 tctgctcgct gagcttgggc tcccgttttc tgtgcgggcc gtggacatcg atgaggagca
     3121 cgccctggcc acggcgccga gccaatctcc gctcgacctc gccatgcgcg ttgcggccgc
     3181 aaaggcggaa gcctcggtac gtcatgcgaa cgaggtcgta ctcacggccg acacgctcgt
     3241 gatgctcgac ggcaggccgc tcggcaagcc gcgcgacgcc tgcgaagcga agcagatgct
     3301 ggaagctctc cgcggccgtg agcacgtcgt ggtgaccgga gtcgccgtcg tcgccgcagc
     3361 ggaaagattc gtcgaagcga cggaggcgac ggtcacgatg cgcgagtacg cagccgacga
     3421 gatctcccgt tacatagagc ggggggagcc cttcgacaaa gcgggcgcct acgccatcca
     3481 ggacaccgtc ttcgcacccg ttgcatcgta tcgcgggtgc tactgcacga ttgtcggact
     3541 cccgctttgg tgcacgcgcc gcctgctgcg ttcggtggga ctgcgtccgg aagcgcctac
     3601 ccgcgccgtc tgtcgagcct gccccgatcg agaggagggc ggatgacgcg gccatagtgg
     3661 aagggtgcgc aaccgctcgg ctttcgccac ccctaagcca tgccgcacca cgtcttaggg
     3721 gctttcctca tcgccaccgc ggaggttccc ctattctcgc cgatcgtccg cagtcccaat
     3781 ctgaacccga ataccgtcac gccgtgggga aagcgggccc cgagg
//
LOCUS       DATMBB010000062         2356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_111633, whole genome shotgun sequence.
ACCESSION   DATMBB010000062 DATMBB010000000
VERSION     DATMBB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111633"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VNL92_01295"
     CDS             <1..412
                     /locus_tag="VNL92_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWO93377.1"
                     /translation="DAYLATVARNLLRSAHRRRAVEGRRRIEVDLDAIPIDRESVDAR
                     VEREELVRMVHAVIRSELPPALAGTVRAILNGSTPSEIAALQGVSPVTVRTRLMRARA
                     ILRVALRRQLDGAEVELPAAPPRHRFSDLRRSGT"
     gene            complement(392..1516)
                     /gene="arsB"
                     /locus_tag="VNL92_01300"
     CDS             complement(392..1516)
                     /gene="arsB"
                     /locus_tag="VNL92_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWO93378.1"
                     /translation="MAPDDDGTASVVRRLSTLDRFLPLWIFVAMAIGLILGRTFPGLG
                     GALDRVQLAGVSVPIGLGLLWMMYPVLAKVRYESIGRHVANTRLLGTSLFLNWVLGPV
                     VMFALAWAFLPDMPAYRNGLVLIGLARCIAMVLIWNSLARGSSEIAAVLVALNSVFQI
                     LTYSFLGWLFLSEVPAWFGAESAVVNVSMWSIAKSVLIFLGIPLLAGYLTRRILVARR
                     GEAWYTGMFMPRFGPTALIGLLYTIVLMFAMQGDRILQLPLDVLRIALPMVMYFFVMF
                     GLAFALSKWLGFSYPETASLSFTAAGNNFELAIAVAVATFGIGSGEALAAVVGPLIEV
                     PVLVGLVYVSLWARRRFYGYGTGRPMHAPDAEDLLRPASS"
     gene            complement(1605..>2356)
                     /locus_tag="VNL92_01305"
     CDS             complement(1605..>2356)
                     /locus_tag="VNL92_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWO93379.1"
                     /translation="NLPALEAVLKDIAAQRDVASTYHLGDLVGYAPWPNETVQLLRSF
                     DVSGVAGNYDSTVATDYKHCGCKYEDARQEELSHLSYEWTRQHTSVDAKRFLGGLPFR
                     IDLRPLGGHLAGPTVTLIHGNHVMNTVYVFEERPDSFLEKMGHGVGARPGDVICFGHT
                     HKPWHRVVNDVHYVNTGSVGRPKDGDWRAGYVLLDVTAASVNVDVVRVEYDVEAAARA
                     IIASDLPNDFAEYLRTGGRLPAPQASGSATA"
BASE COUNT          443 a          785 c          776 g          352 t
ORIGIN      
        1 ggacgcctac ctggccactg tcgcgcgaaa tctcctccgt tcagcccacc gccgccgagc
       61 cgtcgaaggg agacggcgca tcgaggtcga tctcgatgcc ataccgatcg atcgcgagag
      121 cgtcgacgcg cgtgtcgagc gtgaggagct cgtacgtatg gtgcacgctg tcatcaggtc
      181 ggagctgccg cccgcgctcg ccgggacggt ccgcgcgatt ctcaatggct cgacgccgag
      241 cgagatcgcg gcgcttcaag gcgtcagccc cgtgacggtg cgaacgcggc tcatgcgggc
      301 tcgcgctatc ctccgcgtgg cactccgccg acaactggac ggtgcggaag tggagctccc
      361 cgcagcccca ccgcgccatc gtttctctga tctaagaaga agcgggacgt agcaggtcct
      421 cggcatccgg cgcatgcatc ggacgaccgg tgccgtagcc gtaaaatcgc cgacgcgccc
      481 agagcgaaac gtacaccagc ccgacgagca ctggcacctc gatcagcggc cccacgaccg
      541 ccgcgagcgc ctcgcccgag ccgattccga acgtcgccac ggcgacggcg atggccagct
      601 cgaagttgtt ccccgccgcc gtgaacgaca gcgacgcagt ctccgggtag ctgaacccga
      661 gccacttcga gagcgcgaac gcgaggccga acatcacgaa gaagtacatg accatcggga
      721 gcgcgatccg cagcacgtcg agcgggagct ggaggatgcg gtccccctgc atcgcgaaca
      781 tgagcacgat cgtgtagagc agcccgatga gcgctgtggg cccgaaccgc ggcatgaaca
      841 tgcccgtgta ccacgcctcg ccgcgccggg ccacgaggat tcgccgcgtg agatagccgg
      901 ccagcagcgg gatgccgagg aagatcaaca cgctcttcgc gatcgaccac atcgacacat
      961 tcacgaccgc cgactcggca ccgaaccagg ctggcacctc gctcagaaag agccagccga
     1021 ggaacgagta ggtgaggatc tggaagaccg agttgagcgc cacgagcacc gcggcgatct
     1081 cgctcgagcc gcgcgccagc gagttccaga tcaacaccat cgcgatgcag cgggccagcc
     1141 cgatcagcac gagcccgttg cggtacgccg gcatgtccgg tagaaacgcc cacgcgaggg
     1201 cgaacatcac gacgggcccg agcacccagt tcaggaagag cgacgtgccg agcaagcgcg
     1261 tgttcgccac gtgcctcccg atgctctcgt agcgcacctt tgcgaggacc gggtacatca
     1321 tccagagcag cccgagcccg atgggtaccg aaacgcccgc cagttgcacg cggtcgagcg
     1381 cgccgccgag ccccggaaac gtgcggccga ggatcaagcc gatcgccatg gccacgaaga
     1441 tccatagcgg aaggaaccgg tcgagcgtgg agagacggcg gacgacggag gcggttccat
     1501 cgtcgtcggg tgccagcgct gccgatggcg tgcagccggt gtcggtcggt atctgtgcgg
     1561 acgtcacgat gcgatgagtc ggaggggatc tcgatgagga tccgctatgc agtagcggag
     1621 cctgacgcct gcggtgcggg cagcctgccg ccagtcctga ggtattcggc aaagtcgttc
     1681 ggcaggtcgc tcgcgatgat cgcccgcgct gccgcctcga cgtcgtactc gacacgtacg
     1741 acgtcgacat tcacgctggc agccgtgacg tcgagtagca cgtagcccgc acgccagtcg
     1801 ccgtccttgg ggcggccgac cgagccggta ttgacgtagt gcacgtcgtt cacgactcga
     1861 tgccacggct tgtgcgtgtg accgaaacag atgacatcgc ccggtcgggc gcccaccccg
     1921 tggcccatct tctcgaggaa gctgtccggc cgctcttcga acacgtaaac cgtgttcatc
     1981 acgtggttgc cgtggatgag cgtgaccgtg ggacctgcca gatgtccgcc gagcggccgg
     2041 aggtcgatgc ggaacggcag cccgccgagg aatcgtttcg cgtcgacgct cgtatgctgc
     2101 cgcgtccact cgtagctcag atgcgagagc tcctcctgcc gcgcatcctc gtacttgcag
     2161 ccgcagtgct tgtagtcggt cgccacggtc gagtcgtagt tgccggcgac tccggaaaca
     2221 tcgaatgacc ggaggagttg caccgtctca ttcggccagg gcgcgtagcc gacgaggtct
     2281 cccagatgat acgtcgaggc gacatcccgc tgggcggcga tgtccttgag cacggcctcg
     2341 agcgccggga gattgg
//
LOCUS       DATMBB010000063         6579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_150407, whole genome shotgun sequence.
ACCESSION   DATMBB010000063 DATMBB010000000
VERSION     DATMBB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150407"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..685)
                     /locus_tag="VNL92_01310"
     CDS             complement(17..685)
                     /locus_tag="VNL92_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWO93380.1"
                     /translation="MQLRAPTRTEDLLERVRLDLVHNAFPPGSRVTEEAIAERYGVSR
                     TPVREVLRVLAREALLSYLPRKGYVVPTMDPDEMEDLYVVRVGIEEQVAARLHRSADV
                     QMLVGLQEVWSSPPEGTGGNLNVVFADEHFHESLAIASGSTALPPLLRNINQRLHPLR
                     IHDFEDAERVQRTFEQHAAIVSSVLAGDTRLAQAQLRAHILQSYAFVRSRVFRSPAEV
                     EGSH"
     regulatory      965..1064
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            1174..2214
                     /locus_tag="VNL92_01315"
     CDS             1174..2214
                     /locus_tag="VNL92_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015192256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93381.1"
                     /translation="MVLLILALGLGVACGDDDDDDDDVTSSPSAGTTTAGESSGDTIR
                     LGFSAWPGWFPWQVAEEAGIFDDVGIDVEMVWFEGYLDSINAFAAGQLDGNSQTLNDT
                     LASISAGSDQVVVLVNDNSTGNDQIIATADIQRIEDLAGKQIGVEVGVVDHFLLLLGL
                     ESVGLSEDDVNIVNLETGAAAASFAAGQLDAVGVFAPFTTRALEREGAHALFTSADFP
                     GSIPDHLVLSRELVESRPDDVQKLVEAWFRTLEYIEENPDEALEIMAERAGVSVEDYR
                     SYEEGTTIFSAEQNLEAFAPGDDFTSLQFAAEEIADFLVASGLAEERPETDGIFEPRF
                     VEAYVEGNAGAE"
     gene            2214..3098
                     /locus_tag="VNL92_01320"
     CDS             2214..3098
                     /locus_tag="VNL92_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93382.1"
                     /translation="MRRSAVQEPSRAAIERRREAGYLRERRRGRSRKPSPLWRVRDDL
                     PRRITWPLIGLSVALPLLIWMALYASGTVSRIFLPSPLAVADAAVDLARSGDLAADTW
                     ASVRRIAIGFGIAVAISVPLGLAMGAYRSVQSLFEPAIALVRYMPATAFVPLLLIWLG
                     LGESPKIALIVIGTVFFNTLMTANVVWQVPMELIKVSYTLGASSFTVFRKVIFPYAIP
                     GMIDAARVNLAAAWNLIVVAELLAASEGLGFRITRAQRFLNIDQIFVVLAVIGLLGLA
                     SDLGLRMLRNRIAPWSQE"
     gene            3110..3880
                     /locus_tag="VNL92_01325"
     CDS             3110..3880
                     /locus_tag="VNL92_01325"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93383.1"
                     /translation="MEPKLRISGLTKAFPAKPVSATALEGVDLYAEEGEFVCIVGASG
                     CGKSTLLSIVAGLTEPTMGEVLVDGERILGPGPDRGLVFQGYSLYPWRTVAQNVAFGL
                     ELTDLSKEEIRERVRYYLDVMNLTKWADARPSQLSGGMRQRVAIARSLVTEPEILLLD
                     EPFGALDAQTRSSMQTFLRDLWIKTGTTILLVTHDVEEAIYLGQRVYVFASHPGRVKR
                     EIVVPFPIERGAAIRRDPQFLDLREEIYALLAPEGVEV"
     gene            3928..5073
                     /locus_tag="VNL92_01330"
     CDS             3928..5073
                     /locus_tag="VNL92_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HWO93384.1"
                     /translation="MYSVAADHQYRGEVPLHERYGAEARYAVEAESLLPVSKYQEEIA
                     RAIEYGLPAAESIQDRRISTFSRGELPHFAGINTFLKAPYVEDVRRCGEFDVAIIGAP
                     FDGGTTYRPGTRFGPQGIRKISALYGPYSFELGVDLRESISMADLGDVFTIPANIEKT
                     FDQISRAVSHVYASGALPVVLGGDHSIGFATVRGVAKHLRGNLGIIHFDRHVDTQETD
                     LDERMHTTPWFHATNIPNVPPRNLVQIGIGGWQAPRPGVAEGRKRETTIFTVTDCVTM
                     GIEQAAERALDIAWDGAEAVWLSFDVDCLDAAFVPGTGWPEPGGFLPREVLRFIQLIA
                     ERPLAGIEVVECAPPYDNAEITSLLATRVICDTLGCLVRAGHLPKQE"
     gene            5093..5395
                     /locus_tag="VNL92_01335"
     CDS             5093..5395
                     /locus_tag="VNL92_01335"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HWO93385.1"
                     /translation="MRLTPREQDALLIFVAADVARRRRQRGLRLNYPEAVALISEAMI
                     EEARAGRSVDEAAAAGAQVLTVDDVMDGIADMVPVVQVEATFPDGTKLVSCHNPIR"
     gene            5405..5779
                     /gene="ureB"
                     /locus_tag="VNL92_01340"
     CDS             5405..5779
                     /gene="ureB"
                     /locus_tag="VNL92_01340"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWO93386.1"
                     /translation="MNGPYLLDSGDIELNVGRVRRALRVANTGDRPIQIGSHAHFFEV
                     NRALVFDRIGAYGMRLDIPSGTAIRFEPGDVREVTLVELAGRRIARGMNALVEGPTAH
                     DQTRSDALRRARSVGFIAEDAP"
     gene            5776..>6579
                     /locus_tag="VNL92_01345"
     CDS             5776..>6579
                     /locus_tag="VNL92_01345"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007776517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWO93387.1"
                     /translation="MTVISRERYAALYGPTTGDRVRLADTNLVCRIEQDFAVYGDEAV
                     FGGGKSIRDGMAQAPWATSAGGAPDTVITGAIVLDPVLGVVKGDIGIKDGRIVAVGKA
                     GNPDIMDGVHPALVIGPGTEIISGEHMIATPGGIDTHIHMICPQQAWAALSNGITTML
                     GGGTGPAEGTRATTCTPGPWHIERMLQAAEELPVNWGLLAKGNASDIEPLREQVLAGA
                     AGLKIHEDWGATPAVIDAALRVADEFDVQVAIHTDTLNEAGFAEDTIRAI"
BASE COUNT         1150 a         2084 c         2195 g         1150 t
ORIGIN      
        1 cgacgaccgc ggccattcag tgactccctt cgacttcggc cggtgagcgg aacactcgag
       61 agcggacgaa agcgtagctc tgcaggatat gagcacggag ttgcgcctga gcgaggcgcg
      121 tatcgcccgc tagtaccgag gagacaatgg cggcatgctg ctcaaacgtc cgttgcacgc
      181 gttcggcgtc ctcgaagtca tgaatacgaa gtggatgaag gcgctggttg atgttgcgga
      241 ggaggggcgg gagcgccgtg cttccgctcg caatcgcgag gctctcatgg aagtgctcgt
      301 cggcgaagac gacgttgagg ttgcctcccg ttccctccgg gggcgacgac cagacttcct
      361 ggagcccgac gagcatctgc acgtctgcac tcctgtgtag ccgtgcggcc acctgctcct
      421 cgattccgac gcggacgacg tacaggtcct ccatctcgtc gggatccatg gtcgggacga
      481 catagccttt tcgaggcaaa tagctcagca gcgcttcgcg cgcgagcacg cgcagcacct
      541 cacgtaccgg cgtgcggctg acgccatatc gctcggcgat ggcctcctcc gtgacgcgac
      601 tgcctggcgg aaaggcgttg tgcaccaggt cgagccgtac gcgctcgagg aggtcctcgg
      661 tccgtgttgg tgcgcgcaat tgcatacagt cgggaaatta cgggccgcgt gttacgggca
      721 gattgcggat tcgttaagat gaaaagacat gcccttgcaa ggcggcggcc gggcgcattg
      781 catgcactgg tgcacattgg ctgtcctcgt tatcgaggct gtccccgatc cgttaggtcg
      841 ttccgctcgc gatcgagggt gcggatggca gccggcaact ctcgacctgg cgatgcgccc
      901 gcgcctgtca cgccatcttc atcagcctgc aacaacccgg taacacgccg cgcatagcgt
      961 ttcccatgaa cggcgctagg gttccgctcc gttacggagg actggtccga gagcgcagcc
     1021 cgccaaggtc ggcgggtaca cggcgggata aaagcccggg aggtcgatga cccccgggct
     1081 gttctttttc ccgcggaatg gcgaaaacgc ccgcgttggg tggcagggaa aggcaggcaa
     1141 gcggtgactc tgcggcgatg gacaactgca accatggtgt tgctcatcct cgcgctcggg
     1201 ttgggggtgg cgtgcggcga tgacgatgat gacgatgacg acgtgacctc gtccccgtcc
     1261 gcggggacta cgacagcagg cgaatcgtcg ggcgacacga tacgcctcgg gttcagtgcg
     1321 tggccgggat ggttcccctg gcaggttgcc gaagaggcgg gcatcttcga tgacgtcggt
     1381 atcgacgtgg agatggtgtg gttcgagggc tatctcgact cgatcaacgc gttcgccgcg
     1441 gggcagctcg atggaaacag ccagacactg aacgataccc tggcctcgat ctccgccggc
     1501 tcggaccagg tcgttgttct cgtcaacgac aactcgaccg ggaacgacca gatcatcgcg
     1561 acggcggaca tccagcgcat cgaggacctc gccgggaagc agatcggcgt cgaggtcggc
     1621 gtggtcgatc acttcctgct gctgcttggc ctggaatccg tcggcctaag cgaggacgac
     1681 gtgaacatcg tgaatctgga gacgggcgct gcggcggcct ccttcgccgc cggccagctc
     1741 gacgcggtcg gagtcttcgc acccttcacg acccgggcac tcgagcgaga aggtgcgcac
     1801 gcgctcttca cgtcggccga tttccccggg tcgatccccg accatctcgt gctgtcgagg
     1861 gagctcgtcg agagcaggcc ggacgacgtt cagaagctcg tggaagcctg gttccgaacg
     1921 ctcgaataca tcgaggagaa tcccgacgag gccctcgaaa tcatggccga gcgtgccggt
     1981 gtctccgtcg aggactaccg ttcctacgaa gaaggaacca ccatcttcag tgccgagcag
     2041 aacctggagg cgttcgctcc cggcgacgat ttcaccagcc tccagttcgc cgcggaggag
     2101 atcgccgact tcctcgtcgc ctcgggcctc gctgaggagc ggccggagac ggacggcatc
     2161 ttcgagccga gattcgtcga ggcgtacgtc gaaggcaacg ccggagcgga gtgatgaggc
     2221 gcagcgccgt gcaggaacca tcacgtgcgg caatcgagcg ccgcagagaa gccggctacc
     2281 tccgagagcg gaggaggggc cggtcccgga agccctcgcc actctggcga gtccgcgacg
     2341 atctgccgcg ccgtatcacg tggccgctca tcggactgtc ggtagcgctg ccgctcctca
     2401 tctggatggc gctctacgct tccggaacgg taagccgcat cttcctgccc agccccctcg
     2461 cggtggccga cgccgcggtc gacctcgcgc gaagcggcga tcttgcggcc gatacgtggg
     2521 cgagcgtccg gcgcatcgcc atcggattcg gaatcgcggt cgcgatctcc gtgccgctcg
     2581 ggcttgcgat gggagcatat cgcagcgtgc agtcgctctt cgaaccggcc atcgcgctcg
     2641 ttcgctacat gccggccacg gcgttcgtgc cgttgctgct catctggctg ggcctcggcg
     2701 agtcaccgaa gattgcgctc atcgtcatcg ggaccgtgtt cttcaatacg ctgatgacgg
     2761 cgaacgtggt gtggcaggtt cccatggagc tgatcaaggt ctcgtacaca cttggcgcca
     2821 gcagctttac ggtattccgg aaggtcatct tcccgtacgc cattcccggg atgatcgatg
     2881 ccgcacgggt gaacctcgcg gcggcgtgga acctcatcgt ggtggcagag ctgctcgccg
     2941 ccagcgaggg actggggttc cgcatcacgc gggcgcagcg gtttctgaac atcgatcaga
     3001 tcttcgtggt gctcgccgtc atcggcctcc tcgggctcgc ctccgatctc ggactgcgaa
     3061 tgctgcgcaa ccggatcgca ccgtggtcgc aggaataggg gcggcgggta tggaaccaaa
     3121 gctgcgcatc agcggattga cgaaggcgtt cccggcgaag cccgtgtcgg cgacggcgct
     3181 ggagggtgtc gacctctacg ccgaggaggg cgagttcgtt tgcatcgtcg gggcctccgg
     3241 ctgcggcaag tcgacactgc tctcgatcgt cgccggcctc accgagccga cgatgggtga
     3301 ggtgttggtc gacggcgagc gaatcctcgg cccggggccg gaccgcggac tcgtattcca
     3361 gggctattcg ctgtacccgt ggcgcaccgt cgcccagaac gtagcgtttg ggctggagct
     3421 gaccgacctc agcaaggaag agattcgcga gcgggtccgc tattacctcg acgtcatgaa
     3481 tctaacgaaa tgggccgacg cgcggccaag ccagctttcg gggggaatgc gacaacgggt
     3541 cgcgatagcg agatcgctgg tgacggagcc cgaaatactc ctcctcgacg agccattcgg
     3601 tgcgctcgat gcccagacgc gctcctcgat gcagaccttc cttcgtgacc tctggataaa
     3661 gacgggcacg acgatcctgc tcgtcaccca cgatgtcgaa gaggctatct acctggggca
     3721 gcgcgtctac gtgttcgcct cccatccggg cagggtcaag cgcgagatcg tcgtgccctt
     3781 ccccatcgag cgcggcgccg ccattcgtcg cgacccgcag tttctcgacc tccgagagga
     3841 gatctacgcg ctgcttgcgc ccgaaggagt cgaggtgtga cggaccgctc ggtgagccaa
     3901 gaggagctgc atgcgcagca aggacggatg tattcagtgg ctgcagatca ccaataccgg
     3961 ggcgaagtgc cccttcacga acgctatggc gccgaagcac gctacgcggt cgaggctgaa
     4021 agcctcctgc cagtgtcgaa gtaccaggag gaaatcgcgc gcgccatcga gtacggcttg
     4081 cccgccgccg aatcgattca agatcggcgc atctcgacgt tcagtcgcgg cgagctgccg
     4141 cacttcgcgg gcatcaacac ctttctcaag gcgccctacg tcgaagacgt gcgccggtgt
     4201 ggcgaattcg atgtcgccat catcggcgcg ccattcgatg ggggcaccac gtaccgtccc
     4261 ggaacgcggt tcggaccgca ggggattcgc aagatctcgg cgctctatgg cccgtacagc
     4321 ttcgagttgg gcgtcgacct tcgcgagtcg atctcgatgg cagacctcgg cgacgtgttt
     4381 acgattcccg cgaacatcga gaagacgttc gaccaaatca gccgtgccgt gtcgcacgtc
     4441 tacgcgagcg gcgccctgcc ggtggttctc ggaggcgacc attcgatcgg ctttgcgacc
     4501 gtacggggcg tcgccaaaca tctgcgaggc aacctcggca tcatccactt cgatcgccac
     4561 gtcgatacgc aggagaccga ccttgatgag cggatgcaca cgacgccctg gttccacgcc
     4621 acgaacatcc cgaatgtccc gccgcggaac ctcgtgcaaa tcggaattgg cggctggcag
     4681 gccccgcgac cgggtgtcgc cgagggccgc aaacgagaga cgaccatctt caccgtcacg
     4741 gattgcgtga cgatgggcat cgagcaggcc gcggagcgcg ccctggatat cgcgtgggac
     4801 ggcgcggagg cggtgtggct ctcgttcgat gtcgattgtc tcgacgcggc gttcgtgccg
     4861 ggcacgggct ggccggagcc gggcggcttt ctgccacgcg aggtgcttcg cttcatccag
     4921 ctgattgctg agcggcccct ggccgggatc gaggtcgtcg agtgtgcgcc gccctacgac
     4981 aacgccgaga ttacatcgct ccttgcgaca cgagtcattt gcgatacgct tggctgcctt
     5041 gtgcgagccg gccatctccc caagcaagag tgaggtgtag gaggaaccta tcgtgcgctt
     5101 gaccccacga gagcaggacg ctttgctcat cttcgtcgcg gccgacgttg ctcgacgccg
     5161 gaggcagcga ggcctccgcc tcaactaccc cgaggccgtc gcgctgatca gcgaggcgat
     5221 gatcgaggaa gcccgcgcag gccgctcggt cgacgaggct gcggcggcgg gagcgcaggt
     5281 tctaacggtc gacgatgtca tggacggcat cgccgacatg gtgcccgtcg tccaggtgga
     5341 ggccaccttt cccgatggca cgaagctcgt ctcctgccac aatccgatcc gttgaggtga
     5401 ggtcgtgaat ggtccctacc tgctcgatag tggcgacatc gaactgaacg tagggcgcgt
     5461 ccggcgcgcc ctacgcgtcg cgaacacggg cgaccgtccc atccagatcg gctcgcacgc
     5521 ccacttcttc gaggtgaacc gggccctcgt attcgaccgg attggcgctt acggaatgcg
     5581 cctcgatatc ccctctggca ccgccatccg cttcgagccg ggcgatgtcc gggaggtgac
     5641 gctcgtcgag ctcgccggcc ggcggattgc ccgtgggatg aacgcgctcg ttgaagggcc
     5701 gaccgcgcat gaccaaaccc ggagcgatgc cctgcgtcgc gcccgttctg tgggattcat
     5761 cgccgaggat gcgccatgac ggtcatcagc agggagcgct acgcagccct gtatgggccg
     5821 acgacggggg atcgcgtccg gctcgccgac acgaatctcg tctgcagaat cgagcaggac
     5881 ttcgccgtct acggcgacga ggccgtgttc ggcggaggca agtcgatccg cgacgggatg
     5941 gcacaagcgc cgtgggcgac gtccgccggt ggcgccccgg atacggtcat tacgggggcc
     6001 atcgttctcg acccggtgct cggcgtcgtg aagggagaca tcggcatcaa ggacgggcgg
     6061 atcgtcgccg ttgggaaggc gggcaatccg gacatcatgg atggcgtgca cccggcgctc
     6121 gtcatcggcc ccggcaccga gatcatctcc ggtgagcata tgattgcgac gccgggcggc
     6181 atcgatacgc acattcacat gatctgtcca cagcaagcgt gggcagcgct ctcgaatggc
     6241 atcaccacga tgctgggcgg ggggacgggc ccggccgaag gaacgcgggc caccacctgc
     6301 acgcccggcc cctggcacat cgagcggatg ctgcaggcgg cggaggagct gcccgtcaac
     6361 tgggggctgc tcgccaaggg gaacgcctcg gacatcgagc ccttgcgcga acaggtactc
     6421 gccggcgcgg cggggctgaa gatccatgaa gactggggcg ccacgccggc cgtcatcgac
     6481 gcggcgctgc gggtggccga cgagttcgac gttcaggtgg cgatacacac cgatacgctg
     6541 aacgaggccg gcttcgccga ggacaccatt cgggccatt
//
LOCUS       DATMBB010000064         3323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_496547, whole genome shotgun sequence.
ACCESSION   DATMBB010000064 DATMBB010000000
VERSION     DATMBB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496547"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1403
                     /locus_tag="VNL92_01350"
     CDS             408..1403
                     /locus_tag="VNL92_01350"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HWO93388.1"
                     /translation="MHAQDPETARYGMQVLQLPAALGFGDVASQPGFTDSPDGLFGAI
                     HDALGDLEDLGLVEDRGQWSFKLPRHAQGSDDVTLATSWHRIFDEVQPNNREMELLRY
                     AAEAGVVESEEWARSQWLLASKVFPAIGWEFTMQSAAHVTDGLAELRCVTSRPTLGEV
                     RFAPTYIGIVRATETPAFVKSPVPLGAPTCFISYAHEDQIVARDIAYGLQGAELGVEI
                     DEELLAGGDSIVRRVAETTKAVEFVIALVSSHSVASNWCKFELALAMTDELELPGTKV
                     IPVRVDEVKMPALLRDKLYRDVQRDGIDGVIKLLVRDIRKHRERRSGNAEPSGRS"
     gene            1403..1645
                     /locus_tag="VNL92_01355"
     CDS             1403..1645
                     /locus_tag="VNL92_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93389.1"
                     /translation="MVHGPTAVVWVDGWMHANVRHAVNRAFMEMQGTWDLDDGESRER
                     LLALIRAEDFADAELEYDPADGALPRIYLWPRGRTG"
     gene            complement(1729..2769)
                     /locus_tag="VNL92_01360"
     CDS             complement(1729..2769)
                     /locus_tag="VNL92_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93390.1"
                     /translation="MLAGEDFDVADGEAAPEDLVVSIATDVPVHASLTGGRADDILLT
                     PYIKVVSMIGPEVAAEVVDAVGKLRGLREAALPYLRRGVDRYLEGDLPGAIDAAAAAF
                     EDRPAEIKLEAATLLRAFLEDLYQPLGGADAIDAAIAETEAIIRAAKARDATATAALL
                     TALESGPLQEHRRRVLDTALRVLADVDALTSALWSALLETRLPAEELLALRVMRDDFD
                     VLKSRYQDAFELGSMTLGMIAPFANITLRGDVRSFADGQRRSLKDALGARAAVREGWL
                     VDLPAAKVLFDAVSRRTRNDIGHRLVRYDFNSGDLVYDDGAHETYLDFVVDYLNAARL
                     SRYLADIVLRLP"
     gene            complement(3016..>3323)
                     /locus_tag="VNL92_01365"
     CDS             complement(3016..>3323)
                     /locus_tag="VNL92_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93391.1"
                     /translation="RTLDGGKIEFGQQAGVTFGFAPTDRQPELRALMAKGYACAIGVN
                     DLLGIVAYAPPGGWAVPSVEAVRIWPTAAQALSRLELPLIENLFEAVLLQGSLPGAR"
BASE COUNT          593 a         1064 c         1102 g          564 t
ORIGIN      
        1 cggtcctctg cttcctcggg gccgagtggc cgctgttcgg tgctccagac gaacttccac
       61 ggctgcggct ggagcgcccg cgctcgatca agcgcctgct gacgacttcg acgatcctcc
      121 acgacgtcgc ggcggagcgg atcacgtccg tgctctcgtc ggcgctcccg ccgaagtgag
      181 acgctgcttg gtctccccgt tggcaagcgg cccgaccacg agacggtgcg acccaccagc
      241 gactctgcga catggtgcag gcagtcgccc cgatcgtttg gacgaggcgc gaacccctgc
      301 cgcttcagcc cgcgacgcga cagacgagct gtcgatccag tgtcgtaggc tccggatcaa
      361 tgaagtacgt ggcctgggtg gatcgcgttc tgaacgcggc ccggcggttg catgcccaag
      421 accctgagac ggcccgctac ggcatgcagg tccttcagtt gcccgccgcc cttgggttcg
      481 gcgacgtcgc gagccagcct ggtttcacag actcaccaga cggcctcttc ggcgcaatcc
      541 atgatgcgct tggcgacctc gaggacctcg gcttggtcga ggatcggggt cagtggagct
      601 tcaagcttcc tcgtcacgcg cagggctccg acgacgtgac gctcgcgacg tcctggcacc
      661 gaattttcga tgaggtgcaa ccgaacaacc gtgagatgga gctgctgaga tacgcggccg
      721 aagcaggcgt cgtcgagtcc gaggagtggg cacggtccca gtggctgctc gcgtcgaagg
      781 tctttcccgc catcggctgg gagttcacga tgcagagtgc ggctcacgta actgatggcc
      841 tcgccgagtt gcggtgtgtc acgtcgaggc cgacgctcgg cgaggtgcgt ttcgcaccca
      901 cctacatcgg tatcgtgcgg gcgaccgaga cgcctgcatt cgtgaagtcg cccgtccccc
      961 tcggtgctcc gacgtgtttc atcagttacg cccacgagga tcagatcgtg gcgcgcgaca
     1021 tcgcctacgg cctgcagggc gccgaactgg gcgtcgagat cgatgaggag ttgcttgccg
     1081 gaggcgattc gatcgtgcgt cgcgtagccg agacgacaaa ggccgtcgag ttcgtaatcg
     1141 cactggtgtc aagccattcc gttgcctcga actggtgcaa gttcgagctg gccttggcca
     1201 tgaccgacga gctcgaactg cccggcacga aggtgatccc agtccgggtg gacgaagtca
     1261 agatgccagc cctgctccgc gacaagctct atcgcgacgt ccagcgagac ggcatcgacg
     1321 gtgtcatcaa gctgctcgtg cgggacatcc gaaagcatcg ggagcgtcgt tcgggcaacg
     1381 cagagccatc ggggaggtcg tgatggtgca cggtccaacg gcagtcgtgt gggtggatgg
     1441 ctggatgcat gcaaacgtcc ggcacgccgt caaccgcgca tttatggaga tgcagggcac
     1501 ctgggatcta gacgacggcg agagccggga acggttgctg gcgctgattc gtgccgaaga
     1561 cttcgcggac gcggagcttg agtacgaccc ggccgacggc gccctccccc gcatctacct
     1621 gtggccgcgc ggccgcacgg gatgacgaac gtcgggcctc aaccctgatc cacaggccgg
     1681 agtatcgcgt cccatccggc ggaatgggag ggagacattc ggggggcgtc aaggtaggcg
     1741 gaggacgatg tcagcgagat agcgcgacaa cctcgcggcg ttgaggtagt cgaccacgaa
     1801 gtcgaggtac gtctcgtgcg cgccgtcgtc gtagaccaga tcgccggagt tgaagtcgta
     1861 ccggacgagg cggtgcccga tgtcgttgcg ggttcggcgg ctcacggcgt cgaacagaac
     1921 cttggcggca ggcagatcta ccagccaacc ttcccgtacg gcggcacggg cgcccagcgc
     1981 atccttcagg ctccggcgtt gcccgtcggc aaacgatcgg acatctccgc ggagcgtgat
     2041 gttcgcgaaa ggtgcgatca tcccgagcgt catcgagccg agctcgaagg cgtcctgata
     2101 ccgagattta aggacgtcga aatcgtcgcg catcaccctt agagcgagaa gctcctcagc
     2161 cggtagcctg gtttccagga gcgccgacca aagagcggac gtgagtgcgt cgacgtcagc
     2221 caggacgcgg agcgcggtat cgagcacccg cctgcggtgc tcttgaaggg ggccgctctc
     2281 gagtgcggtg agcaaggccg cagtcgcagt ggcgtcgcgc gccttggccg cgcggatgat
     2341 cgcttcggtt tcggcgatcg cggcgtcgat ggcgtccgca ccaccgagcg gctggtaaag
     2401 gtcctccagg aaagctcgaa ggagcgtcgc cgcttcaagc ttgatctcgg cgggccgatc
     2461 ttcaaaggcg gcggcagcag cgtcgattgc gccgggcagg tcgccttcca ggtagcgatc
     2521 aactcccctg cggaggtatg gcaacgccgc ttcccggagt ccacgaagct ttcctacagc
     2581 atcaaccact tcagctgcga cttcgggccc gatcatggag acgaccttga tgtagggggt
     2641 aagcaagata tcgtcagccc gacccccggt tagcgaagcg tgcacgggca cgtcagtcgc
     2701 gatactcacg acaagatcct cgggtgccgc ctcgccgtcg gcgacgtcaa agtcctctcc
     2761 ggccagcatc ggcgagagct cccctccatg tagggcgcca tgcattcccg aacgacaagc
     2821 ggggcaggcg aagaggaaag gctgcaccgt cctcgggtcg tccggggaca gacctgccga
     2881 gacctggatc cgcaggacga gccgtcgtcc gcaaccgcgg cactggacta ggtacctcga
     2941 gaccacggcc ctgattctct cctttcgacg ccttcgctcc ggcagggccg gccaactaga
     3001 cggcgcgcga actggctagc gcgccccggg aagggatcct tgcagcaaga cggcctcgaa
     3061 gaggttctca atcaggggca gctcgaggcg tgacagtgct tgtgccgccg ttggccagat
     3121 ccgaaccgct tcgacggacg ggaccgccca gccaccgggt ggcgcgtagg ccacgatgcc
     3181 caacaggtcg ttgaccccga tcgcgcatgc gtatcccttg gccatgagcg cccgaagctc
     3241 gggctgccga tccgtcgggg caaaaccgaa cgtcacccca gcctgctgtc cgaattcgat
     3301 cttgccccca tcgagcgttc gcc
//
LOCUS       DATMBB010000065         2594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_186213, whole genome shotgun sequence.
ACCESSION   DATMBB010000065 DATMBB010000000
VERSION     DATMBB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186213"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..2140)
                     /locus_tag="VNL92_01370"
     CDS             complement(221..2140)
                     /locus_tag="VNL92_01370"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HWO93392.1"
                     /translation="MSDRARYGWESDFPAFHTAKPMHVRERLQAFIPDASPEQIRAWN
                     ETIPPLQREVEEVLVRDALARHYSTILEYELPLESRRPDAVLLVGDGVLVVELKGKLE
                     PSQADIDQAAAYARDLRCYHRDCNGRPVVAVLVPTRARGYLRQEGQVHVTGPDALDAL
                     VARLTASGSGRTIDRARFLEADAYRPLPSLVEAARELFHSGEIRTIHRARAATDPAVE
                     TISEIIHDAARTRTRHLILVTGVPGAGKTLVGLRTAHAHFLDDLAVPRADGKPTAPAV
                     FLSGNGPLVEVLQYEFRSAGGGGKVFVRGVKDYVRTYSSARRPIPPEHVLIFDEAQRA
                     FDADMVARKHAGRTSAARSEPEHFIEFAERIPDWCVVMGLIGSGQEIHVGEEAGLIQW
                     RWAVERSNRRGDWTVHAPLAAQDVFEDSQIPFDLRPSLNLDTELRFHLAKDLHRFVAG
                     VLAAEPPSALTVLSAGLENEGFHLRLTRSLESAKDYLRSRYADDRDARYGVVASSKDR
                     DLVGFGIPNDFQSTKRVRVGPWYGDPEDEARGLSCRRLTTCVTEFGAQGLELDATLLA
                     WGTDFIMQNGRWSNANARGYRKGVKVRDPFQLRVNAYRVLLTRGREACVVFVPPLPQL
                     DETFDYLQKVGFKLL"
BASE COUNT          445 a          832 c          861 g          456 t
ORIGIN      
        1 tgtcgctggc gcagggcgga gaaggactgg gcgcgtgctg gggcgaggag aaggcgcgcg
       61 agctgctggc cgaggcaggg ttcacgtccg tcgaggtgtc gaagctggag ggagatccgc
      121 tcaacgcgta ctacgtctgc cggccctaga gcggtcggct tactccgctc cgtggcgatg
      181 ggaggcggtg cgattcccgg gcagcgagcc gaccgcctgc tcagaggagc ttgaacccga
      241 ccttctggag gtagtcgaat gtctcgtcga gctgcggcag cggcggtacg aagacgacgc
      301 acgcctcgcg gcctctggtg agcagcacgc ggtaggcatt cacgcgcaat tggaatggat
      361 cccgaacttt cacgcctttt cgataaccgc gagcgttggc gttcgaccat cggccgttct
      421 gcatgatgaa gtccgtgccc cacgccagca gggttgcgtc cagctcgagc ccctgcgcac
      481 cgaattcggt cacgcacgtc gtcaggcggc ggcagctcag gccgcgggcc tcgtcctccg
      541 gatcgccgta ccacggtcct acgcggaccc gtttcgtcga ctgaaaatcg ttgggaatac
      601 cgaacccgac cagatcccga tccttggatg aggcgaccac gccgtagcga gcgtccctgt
      661 cgtcggcgta ccgcgatcgc agatagtctt tcgccgactc cagcgagcgt gtcaggcgga
      721 gatggaaccc ctcgttctcc agtccagcgc tgagcaccgt gagagcagat ggcggctcag
      781 cggcgagtac accggcgacg aaccgatgaa ggtctttcgc gaggtggaac cgcagctcgg
      841 tatccaggtt cagtgagggc cgcaggtcga aaggaatctg gctgtcctcg aagacgtcct
      901 gtgcggccag cggagcgtgg acggtccaat ccccacgccg gttcgatcgt tcgactgccc
      961 accgccattg aatcagtccg gcctcttcgc cgacgtgaat ttcctggccg ctgccgatga
     1021 gacccatgac cacgcaccag tccgggatgc gttcagcgaa ctcgatgaag tgctcgggct
     1081 ccgagcgtgc ggcgctcgtc cgaccggcgt gctttcttgc caccatgtcg gcatcgaagg
     1141 cccgctgcgc ttcgtcgaag atgaggacgt gctctggtgg tatcgggcgg cgggctgaag
     1201 aatacgtcct gacgtagtcc ttgacgccac gtacgaagac cttccctccg ccgcccgcgc
     1261 ttcgaaactc gtactgcaga acttcgacga gagggccgtt tccgctcaga aagaccgccg
     1321 gtgcggtcgg cttgccgtct gcccgtggaa cggcgaggtc gtcgaggaag tgcgcgtgcg
     1381 ccgttcgcag cccgaccagt gtcttgccgg ctcccggcac tccggtgacg agaatcaggt
     1441 ggcgcgtcct cgtccgcgcc gcgtcatgga tgatttccga gattgtctcg acggccggat
     1501 ccgtcgccgc ccgcgcgcgg tggattgtgc gtatttctcc cgaatggaac agttcgcgcg
     1561 cggcttcgac gagcgacggc agaggacgat acgcgtcggc ctcgaggaac cgtgcgcggt
     1621 cgattgtccg gccggacccg gaggctgtca gccgggccac gagcgcatcc agcgcatccg
     1681 gcccggtaac gtgaacctgt ccttcctgac gaaggtatcc gcgcgcgcgg gtgggaacga
     1741 gcacggcgac gacggggcgc ccgttgcagt cgcggtgata gcagcgcagg tcgcgggcat
     1801 atgccgccgc ctggtcgatg tcggcctgcg acggctcgag cttgcccttc agctcgacga
     1861 cgagcacacc gtcgccgacg agaagaacgg catccggccg tcgtgactcc agcgggagct
     1921 cgtattcgag gatcgtggag tagtgcctgg cgagtgcatc tcgaacgagc acctcctcga
     1981 cttcgcgctg aagcggagga atcgtttcgt tccacgcgcg gatctgctcg ggcgaggcgt
     2041 ccgggatgaa ggcctggagc cgttcgcgaa cgtgcatcgg ctttgccgtg tggaaggcgg
     2101 gaaagtccga ctcccatccg tatcgggcac ggtcactcat cgttctccct aacgcctccg
     2161 tcagcgttga aggatctgac cgcccgctgc caagccgcag gcggtcgctg ccagaagggc
     2221 gcgtcctgtc gacagggaac gctcacgccg gaatcggtcg actcggtttt tcatcgatct
     2281 gcaccggcga ccgccggcgg cctctcagtc tgtgactggc tggcgcaggg cggtgaaatc
     2341 gtctatgcct tgcgtgcaag cgaaattatc actcgtcgag tggatccggg cgcgatacga
     2401 aaacgggccc acgttgactc agtctcctcc gggcacgttg atacgcgacg accgcgcgct
     2461 ccacagcatc gccaggcacc tcacacctgg cgagacgacg cccgagggtc acaaggcgca
     2521 gtcctggttc gcacgcgcct accctgaaac acgtgaattg acagattcca cgcgcaggac
     2581 ccgcgaggac gcgt
//
LOCUS       DATMBB010000066         4071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_545879, whole genome shotgun sequence.
ACCESSION   DATMBB010000066 DATMBB010000000
VERSION     DATMBB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545879"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VNL92_01375"
     CDS             <1..306
                     /locus_tag="VNL92_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HWO93393.1"
                     /translation="VEQAFNMIRPGGLAVVVGVGQVGVNAEVPILAFLQEKKLVGSLY
                     GSGQVHVDIPRLIDLYMDGKLMIDELISKVRPLAEVNEAFEDMKKGAVARSVLNVSA"
     gene            397..1596
                     /locus_tag="VNL92_01380"
     CDS             397..1596
                     /locus_tag="VNL92_01380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93394.1"
                     /translation="MPGPMEGVKVVEFGVWVAAPSAAALLADWGADVIKIEPPEGDPF
                     RGLMAGLSDSGSNPPFELDNRGKRSVALNLRTVEARAIAYQILAEADVFITNARPAAL
                     VRSGLDFPTLSERFPRLIYAQLTGYGADSDERDRAAFDVGAFWARAGIAALLTPDGAD
                     FPMQRGGMGDHTAGAQLCAGICAALYHRERTGEGQRVAVSLARTGVYTIGWDINIALR
                     TGVPPGPMTRRTIPNPLIAPYRAGDGRAFWLLMLQGDRHWPDFCRAVGRPDWEHDERF
                     SNLIARAMNCGALSEEIDRVLATKPYAEWAAIFDREDIWYAPVQGPAEVVDDPVMAQA
                     GAFVDVPLGAGGTARMVATPVDFDGTPWEPGGPVPELGQHTEEVLLELGHDWGEIANL
                     KELGAIP"
     gene            complement(1660..3177)
                     /locus_tag="VNL92_01385"
     CDS             complement(1660..3177)
                     /locus_tag="VNL92_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93395.1"
                     /translation="MTASVAQAEAGVTTVSSRRSPNLLIFVLGLGIFVGGFDQTFIVT
                     ILPDIIDDIDLPVSRFGLLAWIINGYLIGYMVAMPLMGRLADIFGRVRVYLMALALFA
                     GGSVGIALADGLWWIVFFRGVTAIGGGALVPIAMAIAADTLEQERRPLAIGSLSSLDD
                     TSSLLGPLWGAVIATALGWRWLFWLNIVFVIPIAIAVPLLARDQPRGLRRPPVDWVGG
                     LTLTLGLTLLAVGLTDNGGDPRPLAITLVLIASGLALGGVFVRHELRTSHPLIDLRLF
                     REVPVAVANVTYFLTGGALITVMINVPLLTNVLWGGDALDGGLNLMRMMVFMPIGGLV
                     GGYLAIRIGYRATAVVGFAGAALALVFMRAWPESPSSIELWIPLAVAGLCFTLDDAPI
                     VATVLDRASVAHRAASAALLQVVQTLGMIVGTALLATQGLGRFNERASEIFAEQQFEA
                     SDEAYQAAIHSTFEETFLVAAIVMAIAAGLGLYLRGGRAQRFRLGSLFGVRGASE"
     gene            complement(3182..3907)
                     /locus_tag="VNL92_01390"
     CDS             complement(3182..3907)
                     /locus_tag="VNL92_01390"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HWO93396.1"
                     /translation="MSRWLVAFVTGALLIGALAVGCGDDDDDGNAATSTAEERPPSEV
                     LAEAADTFAALDSFHFTLTHENGATPFVFDLDLREAEGDIAAPDSLRTHVKADRGAVT
                     VELDVVNIGEDIWVTDPFSGQWQKIDDPDFDIGDLFDPQAGIPDVLRGLQNAEMDGTE
                     RIGDVETIRFRGTVDGGDIRAIAPIAEEGIEVPVVVWVGRDDGLVHRVRTEGRLSEDE
                     SDEVRRQIDLSEFNEPVEIEPPV"
BASE COUNT          716 a         1301 c         1407 g          647 t
ORIGIN      
        1 gtcgagcaag ccttcaacat gatccggcct ggcggcctcg ccgtcgtcgt cggtgtcggc
       61 caggtcggtg tgaacgccga ggtgccgatc ctcgccttcc tccaggagaa gaagctcgtc
      121 ggcagcctgt acggctccgg gcaggtgcac gtcgacatcc cgcggctcat cgacctctac
      181 atggacggca agctgatgat cgacgagctc atctccaagg tgcggccact cgctgaggtg
      241 aacgaggcct tcgaggacat gaagaagggc gctgtcgccc gctcggtcct gaacgtgagc
      301 gcataaccgc gaacccgagg cacgcgtgat gagcgcccgt ggatgtgata tgcgtccgcg
      361 ggcgctttgt tgtatgagaa ctggcggagg tgcagcatgc cgggaccgat ggaaggggtg
      421 aaggtcgtcg agttcggcgt gtgggtggcc gcgccttccg cggccgcgct ccttgccgac
      481 tggggagccg atgtcatcaa gatcgagcct ccggagggcg atccgttccg tggcctcatg
      541 gccggcctct ccgattcagg aagcaacccg ccgttcgagc tagacaatcg tggcaagcga
      601 agcgtagcgc tgaacctgcg taccgtagag gcgcgtgcca tcgcgtatca gatcctcgcc
      661 gaggccgatg tgttcatcac gaatgcgcgg ccggcagccc tcgtacggag cgggctcgac
      721 ttcccgacgt tgagcgagcg attcccgcgg ctcatctatg ctcagctgac cggctacgga
      781 gccgacagcg acgagcgtga tcgcgcagcc ttcgatgtcg gggcgttttg ggcgcgcgcg
      841 ggcatcgctg cgctcctcac gcccgacggc gcggacttcc cgatgcagcg cggcggcatg
      901 ggcgaccaca cggcgggcgc tcaactgtgc gccggcatct gcgccgccct gtaccatcgg
      961 gagcgcacag gcgagggtca gcgtgtcgcc gtctcgctcg cccggaccgg cgtgtacacg
     1021 ataggctggg acatcaatat cgcgctgcgc acgggcgttc cgccggggcc gatgacgcga
     1081 cggaccatcc ccaatccgct catcgcgccg taccgggccg gcgatggcag ggccttctgg
     1141 cttctcatgt tgcagggcga ccgccactgg ccggacttct gtcgcgctgt cggtcggccg
     1201 gattgggagc acgacgagcg cttctcgaac ctgattgccc gcgcgatgaa ctgcggtgcc
     1261 ctgtctgagg aaatcgatcg ggtcctggcg acgaaacctt atgccgagtg ggcagccatc
     1321 ttcgatcgcg aggatatctg gtacgctccg gttcagggcc ccgcggaggt cgtcgacgac
     1381 ccggtgatgg cgcaggcggg cgctttcgtc gatgtgcctt tgggggccgg cggtaccgct
     1441 cgtatggtcg ccactccagt cgatttcgac gggacgccgt gggagccggg cggtccggtc
     1501 ccggagctgg gccaacacac ggaggaggtg ctgctggagc tgggccacga ctggggggag
     1561 atagcgaacc tcaaagagtt gggcgccatc ccgtaggggg cgaagcgcgg gccgcgaggc
     1621 ccagggggtg cctgcttcac agcccctact ggtagccgat cactcgctcg cgcctcgaac
     1681 accgaaaagc gacccgaggc ggaagcgctg cgcccggccg ccgcgtaggt agaggccgag
     1741 gcccgcagcg atagccatga cgatggcggc cacgaggaac gtctcctcga aggtgctgtg
     1801 aatcgcggct tgatacgcct cgtcgctggc ctcgaactgc tgctcggcga agatctcgct
     1861 ggcgcgctcg ttgaagcggc caaggccttg cgtcgcaagc agcgccgtcc cgacgatcat
     1921 gcccagcgtc tgcaccacct ggagcagggc ggcggaggcg gcgcggtggg ccacgctcgc
     1981 tcgatcgagc accgtcgcga cgatcggcgc gtcgtccagg gtgaagcaca ggccggcgac
     2041 ggccagcggt atccaaagct cgatgctgga gggcgattcg ggccatgccc gcatgaacac
     2101 gagggccagg gccgcgccgg cgaagccaac gaccgctgta gcccggtacc cgatccggat
     2161 cgcgaggtag ccgccgacga ggccaccgat gggcatgaag accatcatcc gcatgaggtt
     2221 gagacctcca tcgagcgcat cgcctcccca gagcacgttc gttagcaggg ggacgttgat
     2281 catgaccgtg atgagcgccc cacccgtgag gaagtacgtc acgttcgcca ccgcaacggg
     2341 gacctctcgg aacaggcgca gatcgatcag cggatgggac gttcggagct cgtgtcgaac
     2401 aaacacgccg cccagggcca agccggacgc gatgaggacg agcgtgatgg cgagaggacg
     2461 cggatcgccg ccgttgtcgg tgaggccgac agccagcagc gtcagcccca gcgtcagcgt
     2521 cagcccgccg acccagtcga cgggagggcg ccttaggccg cgcggttgat cacgggctag
     2581 cagaggcacg gcgatagcga tggggatgac gaatacgatg ttgagccaga agagccatcg
     2641 ccatccgagc gcggtggcga tgacagcgcc ccacagcggc cccagcagac tgctcgtgtc
     2701 gtcgagggag gagagggagc cgatggcgag cggccgccgc tcttgctcga gcgtatcggc
     2761 ggcgatggcc atcgcgatgg gcacgagtgc gccgccgccg atggccgtga cgccgcgaaa
     2821 gaagacgatc caccacagtc catcggcgag cgcgatgcca accgagcctc cggcgaagag
     2881 cgccagcgcc atgaggtaga cgcgcacccg cccgaagatg tccgcgagcc gccccatgag
     2941 cggcatggcg accatgtagc cgatcaggta gccgttgatg atccaggcca ggagcccgaa
     3001 gcggctcacc ggcaggtcga tgtcgtcgat gatgtccggg agaatcgtga cgatgaacgt
     3061 ctgatcgaag ccgccgacga agatgccgag gcccaggacg aaaatcagga ggttggggct
     3121 gcggcggctg ctgacggtcg tgacgccggc ttccgcctga gcaacggacg cggtcatgtt
     3181 cttagaccgg cggttctatc tcgaccggct cgttgaactc ggacaggtcg atctgccggc
     3241 gaacctcgtc ggactcatcc tcgctcagtc ggccttccgt tcgcacgcgg tggacgaggc
     3301 catcgtctcg cccgacccag acaacgaccg gaacctcgat cccttcctca gcgatgggcg
     3361 cgatggctct gatgtcgccg ccgtcgacgg tgccgcggaa ccgaatggtt tcgacgtcgc
     3421 cgatccgctc cgtgccatcc atctcggcat tctgcaggcc tcgcagcacg tcggggatgc
     3481 cggcttgcgg atcgaacaga tcgccgatgt cgaaatccgg atcgtcgatc ttctgccact
     3541 ggccgctgaa ggggtcggtg acccagatgt cctcgccgat gttcacgacg tcgagctcga
     3601 cggtgacggc gccgcgatcg gccttcacgt gggtccgcag actgtcgggc gcggcgatat
     3661 cgccctcggc ttcacggagg tcgagatcga agacgaaggg agtcgcaccg ttctcatgcg
     3721 tcagggtgaa atgaaacgaa tcgagcgcgg cgaacgtatc ggcggcctcg gcgaggacct
     3781 ccgatggcgg gcgctcctcc gcggtcgacg tcgcggcgtt gccgtcgtca tcgtcgtcgc
     3841 cacagccgac ggcgagtgcg ccgatcagca acgctccggt gacgaaggcg accaaccatc
     3901 ggctcatcgc tcgcattatc cacaatcacg aagatatgcg ccacgcgggg cgtctcagcg
     3961 tctcagcgat tctggtaggg cagaaatttg ctcgcaacga ctgcgtatgc cggccggatt
     4021 cgcttgctgg ctgtactgaa aatgaactgc tacgagccga cggcgtggcg g
//
LOCUS       DATMBB010000067         4387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_226132, whole genome shotgun sequence.
ACCESSION   DATMBB010000067 DATMBB010000000
VERSION     DATMBB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226132"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..371)
                     /locus_tag="VNL92_01395"
     CDS             complement(159..371)
                     /locus_tag="VNL92_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWO93397.1"
                     /translation="MAEPATITAYNNGPYVVSGDARIVDQDGNEIETQGASIALCRCG
                     QSGKKPFCDGTHAKVGFQSVLQGGSP"
     gene            complement(425..1360)
                     /gene="mdh"
                     /locus_tag="VNL92_01400"
     CDS             complement(425..1360)
                     /gene="mdh"
                     /locus_tag="VNL92_01400"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWO93398.1"
                     /translation="MRSKVTVVGAGNVGATLAQRLAERDYADVVLVDIVDGLPQGKAL
                     DLREAGPVVGYDSRVVGTNGYDETEGSDVVVITSGVARKPGMSRDDLLATNMGIVRGV
                     TRQVVEASPDCVIIVVSNPLDAMCHVAFEASGFPRERVVGMAGVLDTARYRAFIADEL
                     NVSVEDVQAYVLGGHGDSMVPLTRYTTVAGLPIDRLMPQERIDAIVQRTRDGGAEIVS
                     LLKTGSAFYAPSAAAAQMVDAVMLDKKRILPCATLLTGEYGIDGLYIGVPAKLGAAGV
                     EEVIEIELTDEERSALRQSAEAVRELVEAMRRLSA"
     gene            complement(1457..2539)
                     /locus_tag="VNL92_01405"
     CDS             complement(1457..2539)
                     /locus_tag="VNL92_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HWO93399.1"
                     /translation="MTHNVTLIPGDGTGPEITEATRRVLEATGVAFNWDIQHAGIDVM
                     ETEGTPLPARVLDSIRRNTVALKGPITTPVGTGFRSVNVALRKELDLYACLRPCKWYP
                     GVRSRYENIDLVVVRENTEDLYAGIEFERGSPYIERISQITEESGQAAIRPDAGLSLK
                     PISEFGTRRIVKFAFDYARQNGRRQVTAVHKANIMKFSDGLFLAVAREVAKEYPDIEF
                     QDMIVDNMSMQLVQKPELYDVLVLPNLYGDILSDLCAGLVGGLGVAPGANIGEGAALF
                     EPTHGSAPKYTGLNKVNPMAMMFSGMLMLRYLGETEAADALERAMAEVIAEGKDVTYD
                     MKPDRNDPTAVGTSQVADAIIRKLQA"
     gene            complement(2704..3201)
                     /locus_tag="VNL92_01410"
     CDS             complement(2704..3201)
                     /locus_tag="VNL92_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWO93400.1"
                     /translation="MRVTVNGRTYEVERRPDAVVVEGEVIPVSVSAEGDARTVTINDF
                     SYRVELPPRTDAAELVVRVDGRSFTVRTEGRLRAPVSAPKAAPARRRADAPPGAIVAA
                     MSGRIVSIRVEVGQEVKAGQPLLVLEAMKMENEITAPKDGVVKEVPVAAGARVNEGDV
                     LVVLG"
     gene            complement(3203..>4387)
                     /locus_tag="VNL92_01415"
     CDS             complement(3203..>4387)
                     /locus_tag="VNL92_01415"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HWO93401.1"
                     /translation="EKSHENGIDIFRIFDAVNDLRNLETAMRTAKRVGAHVQGTICYT
                     ISPVHSVDLFVSQAKQLVQMGADSICIKDMAGLLHPFTAAELVARFKDAVDVPVVLHC
                     HCTSGMAEMTYVKAVEAGVDAIDCAISALAQGTSQPPTESVVATFEGHPRDPGLDLDL
                     LAQIAQYFAEIRPKYARFEGGLKGVDAGVLVHQIPGGMISNLMSQLRELNAIDRLPDV
                     LQEMPRVRKDMGYPPLVTPTSQIVGTQAVMNVLQGERYKVIPREVQAYVQGYYGHPPA
                     PLSEELVAKVLSNGQEPIGGRPADLLPPEWEKARAEAGELAKSDEDVLSYALFPQVAA
                     DFLKWRDSGGSETETVAAIVAAMTYHERRTPTPLVSSTNGVGQGSAWRMHGRRLQLRA
                     RR"
BASE COUNT          721 a         1433 c         1406 g          827 t
ORIGIN      
        1 gaccgaatta aaggtggctc gtaccgatga ctctagtata cgcggcgcct gtcgctcgcg
       61 acaagcaccg tcgcgacaag caccacgggc ccatagaagg ggggagccgg ctgaccggct
      121 ccccccttcg acgctccgtc aatgacgagc acgatggcct acggcgagcc gccctggagg
      181 accgactgaa agccgacctt cgcgtgcgtg ccatcgcaga acggcttctt gcccgactga
      241 ccgcatcggc agagcgcgat gctggcgccc tgcgtttcga tctcgtttcc gtcctggtcg
      301 acgatccgcg cgtctccgct gacgacgtaa ggaccgttgt tgtaggccgt tatcgttgct
      361 ggttccgcca tgcgtgtctc cttcgattaa gatggcagga tcatctgtca tgcgctatcg
      421 aatgtcaagc gctaaggcgg cgcatggcct cgacaagctc gcgaaccgcc tcggcggact
      481 ggcgcagcgc ggaacgttcc tcgtcggtga gctcgatctc gatgacctcc tcgactccgg
      541 cggcgccgag cttcgcaggc acgccgatgt agagcccatc gatgccatat tctccggtga
      601 gcagggtggc gcaagggagg atgcgcttct tgtcgagcat gacggcatcg accatctggg
      661 cggccgccgc ggacggcgca tagaatgcgc tccccgtctt gagcaacgaa acgatttcgg
      721 cgcccccgtc gcgtgtccgc tggacgatgg cgtcgatgcg ctcctggggc atcagcctat
      781 cgataggaag gcccgcgacg gttgtgtacc gtgtgagcgg caccatggaa tcgccgtggc
      841 cgccaagaac gtaggcctgc acgtcctcga cggagacatt gagctcgtcg gcgatgaagg
      901 cgcggtagcg ggcggtgtca aggactccag ccatcccaac gacgcgctct cgcgggaagc
      961 ccgatgcctc gaaagcaacg tgacacatcg catccagggg gttgctgacg acgatgatga
     1021 cgcagtcggg tgaggcctcg acgacctgtc gcgtcacgcc ccgcacgatg cccatgttcg
     1081 tcgcgagcag gtcgtcgcgg ctcatgccag gcttgcgcgc cactccggac gtgattacga
     1141 ccacatccga tccctcggtc tcgtcgtagc cgttggtgcc gacgacccga gagtcgtaac
     1201 cgacgacagg cccggcctcg cgcaggtcga gcgccttccc ttgcggcaat ccgtcgacga
     1261 tatcgacgag cacgacgtcg gcgtagtcgc gttcggcgag gcgctgagcc agcgttgccc
     1321 ccacgttgcc ggcgccgacg acagtcactt tgcttcgcac cgattcgctc ctctcctcgt
     1381 ttcggtttgg cagggcccgc gatcggacgg gcgcacgagg ctcgtgcgct gccgctctat
     1441 tggtccccgc gataggctaa gcctgaagct tgcggatgat ggcgtccgcg acctgcgacg
     1501 ttccaaccgc ggttgggtcg ttccggtcgg gtttcatgtc gtacgtgacg tccttgcctt
     1561 cggcgatgac ctcggccatc gcgcgctcca acgcgtccgc ggcctccgtc tcgccgaggt
     1621 agcgaagcat gagcataccc gagaacatca ttgccatcgg gttcaccttg ttgagcccgg
     1681 tgtacttcgg ggcgctgccg tgcgtcggct cgaagagagc ggcgccttca ccgatgttgg
     1741 ctcccggcgc cacgccgagt ccgccgacga gccccgcgca caagtcggag agaatgtcgc
     1801 cgtagagatt gggtaggaca aggacgtcgt acagctcggg cttctgtacg agctgcatgg
     1861 acatgttatc gacgatcatg tcctggaact cgatgtccgg atactcctta gccacttcgc
     1921 gcgcgaccgc gaggaagagg ccatcggaga acttcataat gttcgcctta tgcacggcag
     1981 tgacctggcg gcgcccgttc tgacgcgcgt aatcgaacgc aaacttcacg atgcggcgag
     2041 tgccgaactc cgagatgggc ttgagggaaa gaccggcgtc gggccggatc gcggcttgcc
     2101 cgctttcctc cgtgatttgc gaaatgcgct cgatgtaggg gctgccccgt tcgaactcga
     2161 tgccggcgta caggtcctcg gtgttctccc gcacgacgac gaggtcgatg ttttcgtatc
     2221 gactgcggac gccggggtac cacttgcacg gtcgcaggca ggcgtagagg tcgagctctt
     2281 tgcgaagcgc gacgttcacg ctacggaagc ctgtgcccac cggtgtcgtg atcgggccct
     2341 tgagcgcgac ggtgttgcgc cgaatcgagt cgagcacgcg agcggggagg ggcgtcccct
     2401 ccgtctccat gacgtcgatc cccgcgtgct gaatatccca gttgaacgcg acacccgtcg
     2461 cttcgagcac gcgtcgcgtt gcctcggtga tttcaggtcc cgtaccgtcg cctgggatga
     2521 gcgtgacgtt atgagtcatg aggcgctcct tgtcatctcg ggggaggccc ggtcaggcac
     2581 gggacatctg ctcccgaagg catgggttca ttctgctgcg aacgtcggcc tgtaacaagt
     2641 gggtcggcgc gcaatgtgtg gcgctgcgcg agtcgaaggt catcctccgt cgtgcggcac
     2701 cttctatccc agtacgacca gcacatcgcc ctcgttcacg cgcgcacccg ccgctaccgg
     2761 cacctccttg accacgccgt ccttcggcgc cgtgatctcg ttctccatct tcattgcctc
     2821 gagcacgagc agcggctgac ccgccttgac ctcctgtccc acctcgacgc ggatgctaac
     2881 gatgcgtcca gacatcgcgg caacgatcgc tccgggcgga gcatcggccc gccgtcgtgc
     2941 gggcgccgcc ttcggggcag agacgggagc acgtaggcgg ccttccgtgc gcacggtgaa
     3001 agaccgtccg tcgacccgca cgacgagctc agcggcatcc gttcgcgggg gcagctcgac
     3061 gcgatacgag aagtcgttga tggtgaccgt ccgcgcatca ccttccgcgg agacgctgac
     3121 cgggatgacc tctccctcga cgaccacggc atccggccgg cgctccacct cgtaggtgcg
     3181 cccattgacg gtcactcgca cgctagcgcc tcgcccggag ctggagacgc ctgccgtgca
     3241 ttcgccacgc cgagccctga ccgacaccat tcgtggatga cacgagcggc gttggggtgc
     3301 gccgttcgtg atacgtcatg gctgccacga tggcggcgac cgtctcggtt tcggagccgc
     3361 ccgagtcgcg ccacttcagg aagtccgccg caacctgggg aaagagcgcg tacgaaagga
     3421 catcttcgtc gctcttcgcc agctcgcccg cctcggcccg cgccttctcc cactccggcg
     3481 gcagcagatc ggctggccgg ccgcctatcg gctcctgtcc gttggagagc accttcgcga
     3541 caagctcttc gctgagtggg gcgggtgggt gcccgtaata gccctggacg taggcctgca
     3601 cctcgcgcgg gatgacctta tagcgctcgc cctgcaggac gttcatcact gcctgcgttc
     3661 caacgatctg gctggtcggc gtcacgaggg gtgggtagcc catgtccttc cgcacccgag
     3721 gcatctcctg gagcacgtca gggaggcggt cgatggcgtt gagctcgcga agctggctca
     3781 tgaggttcga gatcatcccg ccggggatct ggtggacgag gactccggca tcaacaccct
     3841 tgagcccgcc ctcgaaccta gcgtacttgg ggcgtatttc cgcgaagtac tgcgctatct
     3901 gggccagcaa gtcgaggtcg aggcctggat cgcgcgggtg cccctcgaac gtcgccacga
     3961 cgctctcggt cggcggttgt gacgtgcctt gcgccaaggc ggaaatcgcg cagtcgatgg
     4021 cgtccacgcc cgcctccacg gccttgacgt aggtcatctc agccatgccg ctcgtacagt
     4081 ggcagtggag cacgaccgga acatcgacgg catccttgaa gcgagcaacg agctcggctg
     4141 ccgtgaaggg atgcagcagc cctgccatgt ccttgatgca gatcgagtcc gcccccatct
     4201 gcacgagttg cttggcctgg gagacgaaca ggtcgacact gtggacgggg ctgatggtgt
     4261 agcagatcgt gccctgtacg tgcgctccga ctcgctttgc cgtacgcatc gccgtctcga
     4321 ggttccggag gtcgttgacg gcatcgaaga tgcggaagat gtcgatgccg ttttcgtggg
     4381 atttctc
//
LOCUS       DATMBB010000068         5522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_226064, whole genome shotgun sequence.
ACCESSION   DATMBB010000068 DATMBB010000000
VERSION     DATMBB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226064"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VNL92_01420"
     CDS             complement(<1..1152)
                     /locus_tag="VNL92_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWO93402.1"
                     /translation="MLKWVGKLLGDSNERELKRAYPIVDDINALEPQYEALRDDDLRD
                     TTQRLRERLAAGDQLDDVLPDAFAAVREASKRHLGMRHFDVQLLGGIVLHSGKIAEMK
                     TGEGKTLVATLPLYLNALEGRGAHLVTVNDYLAKRDAQWMGPIFHALGLTVGVIQHDT
                     SYLFDPAHESENPALQHLRPISRREAYRADITYGTNNEFGFDYLRDNMVTDLGQMVQR
                     ELNYAIVDEVDNILIDEARTPLIISGRASESADVYRTFAQLVPRLQEEEDYTVEEKSR
                     SVLLTENGIENLERWLNIDNIYDPANYRITRYMEAALKAHILYRRDREYVVKDGEVII
                     VDEFTGRLMPGRRYSEGLHQAIEAKEGVPIQHEMVTLATITFQNYFRIYD"
     gene            complement(1174..2133)
                     /locus_tag="VNL92_01425"
     CDS             complement(1174..2133)
                     /locus_tag="VNL92_01425"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWO93403.1"
                     /translation="MGPAAYQDLRIFSGNAHPALTSAICNYLECEPGKIEVFEFSNEN
                     IFVQICENVRQRDTFLVQPIVSPVNTRLVELLIMIDTFRRASAGRITAVIPYYAYGRS
                     DKKDQPRVPITARLIANLIETAGADRVLTVDLHAGQIQGFFNIPVDELTALYEISKYV
                     RTRQIDDLVVVATDVGDSKRARNLAQALDAPLAIIEKRRVGNIERAEALNVIGDVQGR
                     RALIVDDEIDTGGTIVESVGILKEKGARECYAACTHAIFSHPAAERLASTPLEEIVIT
                     DTVPVPESKRAKLPNIQVLSLAPLLGEAIRRIHTGQSIGELFG"
     gene            2378..3001
                     /gene="coaE"
                     /locus_tag="VNL92_01430"
     CDS             2378..3001
                     /gene="coaE"
                     /locus_tag="VNL92_01430"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWO93404.1"
                     /translation="MIKIGLTGGIASGKSTVSAMLLEMGAHIIDADKVGHEAYEPGTK
                     VWEEVVAAFGQDVVGENGAIDRKRLGPIVFNDPDALQRLNAIVHPRMKEMIAQRFEEL
                     ERAGNVQVAVLEAAILIEAHWESLVDEIWVTAVPEEVAKDRLMARNGLTPEQAESRIR
                     SQLSNEERIAHADSIIDTSVTIPEVRVQVERLWGELSERAASASATH"
     gene            3068..3934
                     /locus_tag="VNL92_01435"
     CDS             3068..3934
                     /locus_tag="VNL92_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HWO93405.1"
                     /translation="MEEYASVEEPFYLPIADEVELFESAFSEKIPVLLKGPTGCGKTR
                     FVEYMAFRLGRPLTIVKDRGPAEEPVRAEVGPHGLPIVTVACHEDLTASDLVGRYLLE
                     GESTRWIDGPLTRAVKAGAICYLDEVVEARKDTTVLIHPLTDHRRILPIEKQGQVLEA
                     ADGFLLVISYNPGYQSALKDLKHSTRQRFIAIEFGYPPRDYEAQIIEHEAGVSAQTAN
                     DLAKLGEKVRNLRDHGLAEGVSTRLLVYAGKLMRSGIPARRACQTAVVWALTDDAEVQ
                     RSIEEVVSSIFE"
     gene            3995..4612
                     /locus_tag="VNL92_01440"
     CDS             3995..4612
                     /locus_tag="VNL92_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWO93406.1"
                     /translation="MKLGIVSDIHCNALGLSRAIELMGDVDEILCAGDSIYEFRFSDD
                     VVDLIRQHRVRMVLGNHEAVALGTQGRRVVEKPEVKQDNVAWLRDQPWQLETRVNGKR
                     LLMAHGSPFEPWNEYIFPHTPSFKRLAELETDYVILGHTHQAMAERSGRVLVINPGST
                     GEARDPRIGFQLSFAVLDTQTDEVHIELFPDPQREARERAANGYA"
     gene            4631..>5522
                     /locus_tag="VNL92_01445"
     CDS             4631..>5522
                     /locus_tag="VNL92_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93407.1"
                     /translation="MTQPDLLSVEDELRGYPGNLSEDFRRSAESLSYTLTPEQLAAWA
                     NECLMLAHHSLRAWEATGEYLRASPLVLTRLSFDRFLAWVSHGRDLADLSASIARSYF
                     KASPATLALLPEGELDEWARLGSSLYKGTWKSISLAGQFLELSPRLLAQVDMSEMRTL
                     VAVIDDLAERSYDLAFTCLESAAQSLAAAARVDRPAFLGFASVLAKHTWADTRIFFER
                     GPGLVNQIEPSTRSRYLRLVAGTAPELTRQTYGYFAEGSNALARIDPALHASLVARAE
                     PLAARSGMAAMEFVKSAPEVL"
BASE COUNT          994 a         1679 c         1787 g         1062 t
ORIGIN      
        1 gtcgtagatg cggaagtagt tctggaacgt gatggtcgcg agcgtcacca tctcgtgctg
       61 gatcggcacg ccttccttgg cctcgatggc ctgatggagt ccctccgagt agcgccgacc
      121 cggcatgagg cggcccgtga actcgtcgac gatgatcact tcaccgtcct tcaccacgta
      181 ttcacggtcg cgtcgataga ggatgtgcgc cttgagcgcc gcctccatgt agcgcgtaat
      241 ccggtagttc gccggatcgt agatgttgtc gatgttgagc catcgctcca gattttcgat
      301 tccgttctcc gtgaggagta cggaacgtga tttctcctcg actgtgtagt cttcttcctc
      361 ctggagccgg ggaacgagct gcgcgaacgt gcggtacaca tcggcgctct ctgaggcgcg
      421 gccgctgatg atgaggggcg ttcgcgcttc gtcgatgagg atattgtcca cctcgtcgac
      481 gatggcgtag ttcagctcgc gctgcaccat ctggccgagg tcggtcacca tgttgtcgcg
      541 cagatagtcg aagccgaact cgttgttggt tccgtacgtg atgtcggccc gatacgcctc
      601 tcgccgcgag atcgggcgga ggtgctggag ggcgggattc tccgactcgt gagccgggtc
      661 gaagaggtac gacgtgtcat gctggatgac gccgactgtc agcccgaggg catggaagat
      721 ggggcccatc cattgggcat cgcgcttggc gaggtagtcg ttgacggtga ccaggtgggc
      781 gccgcgccct tcgagggcgt tgagatagag cggcagcgtg gcgacgaggg tcttgccctc
      841 gccggtcttc atctcggcga tcttgccgct gtgcagcacg atgccgccga gcaactgaac
      901 gtcgaaatgg cgcatgccga gatgccgctt gctcgcctcg cgtacagcgg cgaaggcatc
      961 cggcagaaca tcgtcgagct ggtcgcccgc tgcgagccgc tcacgcagcc gttgggttgt
     1021 atctcgcaga tcgtcgtcgc gcagggcctc gtattggggc tcgagcgcgt tgatgtcatc
     1081 gacaatgggg tacgctcgct taagctcgcg ctcgttcgag tctcctaata gcttgccgac
     1141 ccacttcagc atttcctgcg acctccgggc ggcctaaccg aacagctcgc cgatcgactg
     1201 gcccgtgtga atgcggcgaa tcgcttcgcc gaggagcggt gccaacgata gcacttggat
     1261 gttcggcagt ttggcccgct tgctttccgg cactggcacc gtgtcggtga tgacgatctc
     1321 ttcgagcggc gtcgaagcca ggcgttcggc agcagggtgc gagaagatgg catgggtgca
     1381 ggcggcgtag cactcgcgcg cgcccttctc cttcaggatg ccgaccgatt ccacaatagt
     1441 tccgcccgta tctatctcgt cgtcaacgat cagagctcgg cggccctgca cgtcgccgat
     1501 gacgttcagc gcctcggctc gctcgatgtt gccgacgcgg cgcttctcga tgatggcgag
     1561 cggagcgtcg agtgcttggg cgaggtttcg tgcacgcttc gagtcgccga cgtcggtcgc
     1621 gacgacgacg agatcgtcga tctggcgtgt gcgaacgtac ttgctgatct cgtagagggc
     1681 ggtgagctcg tccacaggga tgttgaagaa gccctggatc tgccctgcgt gcaggtcgac
     1741 cgtcagcacg cggtcggccc cagcggtctc gatcaagttc gcgatgagtc gcgcggtgat
     1801 gggaacacgg ggctggtcct tcttatcgga gcgcccgtag gcgtaatacg gtatgacggc
     1861 agtgatgcgg ccggccgaag cacggcggaa cgtgtcgatc atgatgagaa gctccaccag
     1921 ccgggtgttc acgggcgaca cgatgggctg gacgaggaat gtgtcccgtt ggcggacgtt
     1981 ctcgcatatc tgtacgaaga tgttctcgtt gctgaactca aacacctcga tcttgccggg
     2041 ctcgcactcg aggtagttgc agatggcgct cgtgagcgct ggatgggcgt tccccgaaaa
     2101 gattcgcagg tcttgataag cagctggtcc cacagccccg tttccaaaaa agaaggccgg
     2161 tggcgaggta ctgacctcgt cgcgcccggc cgcgtgcgcc ttccggtcgt agcattacta
     2221 taacactctc agccccttgg atgcagcgtc ggcagaggga gccgtaggcg aatctggcct
     2281 aggcacttcg ccgccttgac tcgccgatcg agcggtgcct acaatacttc acgcccgcct
     2341 gaaaatggca catctggcca gagaggaaga cctccgcttg attaagatcg gccttaccgg
     2401 cggcatcgcg agcggcaaga gtaccgtctc cgcaatgctg ctcgagatgg gagcgcacat
     2461 catcgacgcc gacaaggtgg ggcacgaggc gtacgagcct ggaacgaagg tttgggagga
     2521 ggtcgttgcg gccttcggac aagatgtcgt cggggaaaac ggcgccatcg atcgcaagcg
     2581 gctggggccc atcgttttca acgatccgga tgcactgcag cgcctgaacg ccatcgtcca
     2641 tccacggatg aaggaaatga tcgcgcagcg gttcgaggaa ctcgagcgag cgggaaacgt
     2701 gcaggttgcc gtactcgagg cggccatcct catcgaggcg cactgggaat cgctcgtgga
     2761 cgagatttgg gttaccgcgg ttcctgaaga agtggcgaaa gaccgcctta tggctcgaaa
     2821 cggactgact cccgagcagg cggaatctcg catacgcagt cagctcagca acgaagaacg
     2881 catcgcgcac gccgacagca ttatcgatac aagtgtgacg atcccggagg tgcgcgtcca
     2941 agtcgaacga ctttggggcg agctctccga acgcgcggcg tccgcgagtg cgacccacta
     3001 aggccaggga cggcaattga ccaccacttc gaatcccact cagcccgcga cgtaccgaga
     3061 atacgctatc gaggagtacg cctctgtcga ggagccgttc taccttccga tagcggatga
     3121 ggtcgagctg ttcgagtcgg ccttcagcga gaagatcccg gtcttgctga agggccccac
     3181 gggctgcggc aagacgcgct tcgtagagta catggctttt cgccttggcc ggcccctcac
     3241 gatcgtgaag gatcgcgggc ccgccgagga gccggtgcgg gccgaggttg gtccccacgg
     3301 cctccccatc gtcacggtcg cctgtcacga ggacctgacc gcgagcgatc tcgtcgggcg
     3361 gtacttgctg gagggcgaat cgacgcggtg gatcgatggg ccactcaccc gggccgtcaa
     3421 agccggcgcc atctgctatc tcgacgaagt cgtggaagcc cgaaaggaca cgacggtcct
     3481 catccatccg ctcaccgacc atcggcgcat tctgccaatt gaaaagcagg gccaagtgct
     3541 cgaagccgcg gatggcttcc tgctcgtcat ctcgtacaac ccgggctacc aaagcgcgct
     3601 gaaggacctg aagcacagta cgcgccagcg gttcatcgcc atcgagttcg gctatccgcc
     3661 acgtgactac gaggcgcaga tcatcgagca cgaggccggt gtttcggctc agacggcgaa
     3721 cgaccttgcg aagctcggcg agaaggtgcg aaacctgcgc gaccacgggc tcgcggaagg
     3781 cgtgagcacg aggttgctcg tctatgccgg caagctcatg cggtcgggga taccggctcg
     3841 cagggcctgc cagacggctg ttgtgtgggc gttgaccgac gatgcagagg tgcagcgaag
     3901 tatcgaagag gttgtatcct cgatcttcga gtagcggcat cgcttcgaga cgtcatgcca
     3961 tgtgtcttgc gagccctgtc gaaaggagca ccccgtgaag ctcggcattg tctccgatat
     4021 ccactgcaac gcgctcggct tgagccgggc catcgagctc atgggtgacg tcgacgagat
     4081 cctttgcgct ggggactcga tctacgagtt ccgcttctcc gacgatgtgg tcgacctcat
     4141 tcgtcagcac cgcgtacgca tggtgttggg caaccacgaa gcagtggcgc tgggcaccca
     4201 aggccggcga gtggtggaga agcccgaggt caagcaggac aacgtggcgt ggttgcgaga
     4261 ccagccatgg cagctggaga cacgagtgaa cggcaagcgg ctcctcatgg ctcacggcag
     4321 cccatttgag ccgtggaacg aatacatctt tccgcacaca ccgtccttca agcgactcgc
     4381 agagttggag acggactacg tcatcctcgg tcacacgcat caggccatgg ccgagcgctc
     4441 gggccgcgtg ctggtcatca atccgggctc gaccggcgag gcgcgcgatc cgcggattgg
     4501 attccaactc agcttcgctg tcctcgatac gcagaccgac gaggtacaca tcgagctgtt
     4561 tccggatccg cagcgcgagg cgcgggagcg agcggcgaac ggttacgcgt gaggcttccg
     4621 gagaagggcg atgactcaac ccgatttgtt atccgtggag gacgagcttc gggggtaccc
     4681 gggcaatctt tccgaggatt tccggcgtag cgcggaatcg ctttcctaca ccctaacgcc
     4741 agaacaactc gccgcgtggg cgaacgagtg cctcatgctg gctcaccatt ccttgcgggc
     4801 ctgggaggcg accggcgagt acctgcgtgc ctcgccgctc gtgctgactc gcctcagctt
     4861 cgatcgcttc cttgcctggg tttcgcacgg gcgcgacttg gcggaccttt cggcgagcat
     4921 cgctcgttcg tacttcaagg ccagcccggc gactcttgcg cttctgccgg agggcgaact
     4981 cgacgagtgg gcccggcttg gctcctcgct gtacaagggc acgtggaaat ccatctcgct
     5041 cgcgggccag tttctcgagt tgagtccccg cttgctggcg caggtcgaca tgagcgaaat
     5101 gcgaaccctc gtcgccgtca tcgacgacct ggcggagcgc tcgtacgatc tggccttcac
     5161 ttgcctcgag tcggctgccc aatcgctcgc cgccgcggct cgggtcgatc gaccggcgtt
     5221 cctgggcttc gccagcgtgc tcgcgaagca tacctgggcc gatacgcgca tcttcttcga
     5281 gcgtggacct ggcctcgtca accagatcga accttccacg cgatcacgct acttgcgctt
     5341 ggtcgccggt accgcgcccg agctcaccag gcaaacgtac ggctatttcg cagagggctc
     5401 gaacgccctc gcgcgcatcg acccggcact gcacgcgagc cttgtcgcgc gcgccgagcc
     5461 gctcgcggcc cggtcgggca tggccgccat ggagttcgtc aagagtgcac cggaggtgtt
     5521 gg
//
LOCUS       DATMBB010000069         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_307358, whole genome shotgun sequence.
ACCESSION   DATMBB010000069 DATMBB010000000
VERSION     DATMBB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307358"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VNL92_01450"
     CDS             complement(<1..1104)
                     /locus_tag="VNL92_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93408.1"
                     /translation="MSAELDWGKVLAAPPSAPRPATLRDRLLDIDDWVTLAVALTAAI
                     AVAASIQSADWVESMPALPFVAAAGLLLGYLTTRVPLQSAAARAWIAFGTFLSAGAVT
                     VIATTLSSIEQGNAWERWNAFRDRMAAWFDIVVNGGISDDHIPFVLLVVSLTWVVSFA
                     MAWSIFVLRNAWLALVPLALVIIGNNAYRDIEFDAALVLFLFSGILLLGRVAFQRQQA
                     VWSREDIEAPDFVGVRASWQTAWVVAALLGLAWLLPIDSTGDTLGGLWDDIAEPINER
                     IFDNGRFFAGIRGDQAEAFRDFGDTLPLGGSIDLRNRVQLRAEVSEPVPFLRVAAYER
                     YESGGWLTGERTTRAGFESDLPAPAEIADRAADT"
     gene            complement(1101..2372)
                     /locus_tag="VNL92_01455"
     CDS             complement(1101..2372)
                     /locus_tag="VNL92_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWO93409.1"
                     /translation="MYRTKPVIRPRAGFWIGLVFAIVSLGFAAFSGYWVMYRLGYALV
                     GVLLVSYVWTRVSLRGLQAEVDRHTDRVQEGEDIVERITVRNESAIPKLWIEIQDPTE
                     VPSHHARFVVSLPSKGRRSRRVATRCERRGVYSIGPLQIASGDPFGLYSASMSIGPAE
                     SILVYPRPIELTNFEVPAANLPGDGRFRRRTHYVTPNSAGIREYAIGDSFNRIHWPST
                     ARLGQLMVKLFELDPASDIWIALDLQETAQAGEGMDGTEEVGVRIAASIARALLVGNR
                     SVGLLAYGARLHTIEADRGQQQLTHILEALAMAQATGDVSLGDLLFQEGRRFGRHTTV
                     VAVTASLDEQWVTALQGLAQRGVRTAAIYMEPETFGAGESSLLVFGALMAADLPAHFV
                     RRGDDLRAALSPGGQDASRQELISGQKDRQA"
     gene            complement(2397..>2577)
                     /locus_tag="VNL92_01460"
     CDS             complement(2397..>2577)
                     /locus_tag="VNL92_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93410.1"
                     /translation="FVLPDDVKALAYSALGHRVIINPSSRVKNVTAAVVIEECLERIP
                     VPGSRARGGYQAASS"
BASE COUNT          457 a          826 c          868 g          426 t
ORIGIN      
        1 agtatccgcg gcgcggtcgg cgatttccgc cggcgccggc aggtccgatt cgaagcccgc
       61 cctcgtcgtg cgttcgccgg tgagccaacc gccgctttcg tacctctcat acgcggcaac
      121 gcggaggaag gggaccggtt cgctcacctc ggcccgcagt tgcacacggt tgcgaagatc
      181 gatggagccg ccgagcggca gcgtgtcacc gaagtcgcgg aaggcttccg cttggtcgcc
      241 gcgaatgccc gcaaagaagc gtccattgtc gaagatgcgt tcgttgatgg gttcggcgat
      301 gtcgtcccag agtccgccga gcgtgtctcc cgtcgagtcg atgggcagca accatgcgag
      361 gccgaggagg gcggcgacca cccaggctgt ctgccaggac gctcgcacac cgacgaaatc
      421 cggcgcctcg atgtcttcgc ggctccacac tgcctgctgg cgctggaatg ccacgcggcc
      481 caggagcaag atgccgctga acaggaatag cacgagcgcg gcatcgaatt cgatgtcgcg
      541 ataggcgttg ttgccgatga tgaccagggc cagcgggacc agcgccaacc acgcgtttcg
      601 gagtacgaag atgctccacg ccatcgcgaa gctgaccacc cacgtcaggc tcacgacgag
      661 gagaacgaag gggatatgat catcgctgat gccgccgttc acgacgatgt cgaaccaggc
      721 agccatgcga tcgcgaaatg cgttccagcg ttcccaggcg ttcccttgct cgatggatga
      781 cagcgttgtg gcgatgaccg tgacggcgcc agcgctcagg aatgtcccga atgcgatcca
      841 ggctcgcgct gccgccgatt gcaggggcac gcgtgtcgtg aggtatccga gcaggaggcc
      901 ggccgcggcg acgaatggga gcgcgggcat ggactcgacc cagtcggcgc tctggatgga
      961 cgcagcgacg gcgatggcgg cggtgagggc tacggccagc gttacccaat cgtcgatgtc
     1021 gaggaggcgg tcgcgcagcg tcgcggggcg cggcgccgac ggtggcgcgg cgagaacctt
     1081 gccccaatcg agctccgcgc tcatgcctgt cgatccttct gcccggagat cagttcctgc
     1141 cggctcgcgt cttggccgcc gggactgaga gcagcgcgaa ggtcatcgcc gcgccgcacg
     1201 aaatgagccg gcaggtctgc cgccatcaac gccccgaaca ctagcagcga gctttcgccg
     1261 gcaccgaacg tctccggctc catgtagata gcggccgtgc gcacgccgcg ctgggccaag
     1321 ccctgcaggg cggtcaccca ctgctcgtcg agcgacgccg ttaccgccac gacggtggta
     1381 tgacggccga agcggcgtcc ctcctgaaag aggaggtcgc ccagcgagac gtcgccggtt
     1441 gcctgcgcca tggccagcgc ctccaggatg tgagtgagtt gctgctggcc ccggtcggcc
     1501 tcgatggtgt gaagccgcgc gccataggcg aggagcccga ccgatcggtt acccacgagg
     1561 agggcgcgcg cgatggaggc ggcgatgcga acgcctactt cttcggtgcc gtccattccc
     1621 tcgccggcct gcgctgtttc ctggagatcg agtgcgatcc agatgtcgga agccgggtcg
     1681 agctcgaaga gcttgaccat gagctggccc agtcgagctg tagaaggcca gtggatccgg
     1741 ttgaagctat cgccgatcgc gtattcgcgg atgcccgccg agttgggcgt gacgtagtga
     1801 gtcctgcgcc ggaaccggcc gtcgccgggg agattcgcgg cgggcacctc gaagttcgtg
     1861 agctcgatag gccgcggata cacgaggatg gattctgcgg gtccgatgga catcgaggcg
     1921 gaatagagac cgaagggatc gccgctggcg atctgcagcg gcccaatgct gtacactccg
     1981 cggcgctcgc agcgcgttgc gactcgtcgc gaacgccgtc ccttactcgg caggctgacg
     2041 acgaagcgcg catgatggct tggcacttcc gtggggtcct ggatctcgat ccatagcttg
     2101 ggaatcgcac tctcgttgcg caccgtgatg cgctcgacga tgtcttcgcc ctcctggaca
     2161 cggtccgtgt gccgatcgac ctccgcctgg agtcctcgaa ggctcacgcg tgtccacacg
     2221 tagctcacga gcagtacacc aacgagggcg tagcccagtc ggtacatgac ccagtacccg
     2281 ctgaacgcgg cgaagcccaa cgagactatc gcgaagacga ggccgatcca gaatcccgcc
     2341 cgaggccgga tgaccggttt cgtgcggtac atggcaacct ccgtaggcct atcgagttat
     2401 gacgacgctg cttggtatcc gcctctagcg cgcgacccgg gtaccggaat ccgctccagg
     2461 cactcctcga tgacgacggc cgcggtgacg ttcttcactc gcgacgatgg gttgatgatc
     2521 acccggtgcc ccaaggcgga gtaggcgagc gccttgacat cgtccggtag cacgaag
//
LOCUS       DATMBB010000070         7805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_186021, whole genome shotgun sequence.
ACCESSION   DATMBB010000070 DATMBB010000000
VERSION     DATMBB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186021"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VNL92_01465"
     CDS             <1..666
                     /locus_tag="VNL92_01465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93411.1"
                     /translation="DFAWRIVVLNNGSTDDTLERACRIAESCREVGVLHLPEKGRGRA
                     LRHAFQQSDADVRMYMDIDLSTSLDAVLPLVRAITEEGYDLAVGTRLHRDAAVARSLE
                     REAVSQMYNLLVRFVFDTRFSDAQCGFKAVSAKTARVLVPQVKDDEWFFDTELLLLAE
                     RNGYRIKDVPVVWVEDPDSRVNVGRTALQDVRGMARLKFGGIPRIIAAECGREGRTRA
                     FGS"
     gene            947..1702
                     /gene="extP"
                     /locus_tag="VNL92_01470"
     CDS             947..1702
                     /gene="extP"
                     /locus_tag="VNL92_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HWO93412.1"
                     /translation="MAVTEQPAKPSFRERVYDRLFHNYIWRSIFRSGYPNTPRNQMLV
                     VATNVFLHLHPTRIHRTHVKITHTYCLGGLSFFMFLGLTVTGVLLMFYYIPSTEQAYA
                     NMQALETDVQFGMLIRNLHRWMAHAMVILVFLHMMRVFYTGAYKPPREFNWVVGVVLL
                     VLTLLLSFTGYLLPWDQLSIWAITVGSEIAGSAPVLGDDVKFTLFGGFSIGQTSLIRF
                     YTLHVIALPLLAAVFMAVHFWRIRRDGGMARPL"
     gene            1736..2620
                     /locus_tag="VNL92_01475"
     CDS             1736..2620
                     /locus_tag="VNL92_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome C reductase"
                     /protein_id="HWO93413.1"
                     /translation="MAEQTATRPRPRREREEEVLVWPDLVFVEFIAAVLFTITLILLS
                     TLVNAPLLDRANPDVTPNPSKAPWYLLNLQELLLHMEPGLAGVVIPTVALVGLAAIPY
                     IDRKEEGRGVWFATPNAVKLTIFAWVYTVVLTVVLILFDAGKFESYTEWFPGLPDGQG
                     LSGTRTLQTSWSWSIGDFDYPEDLEAVGAPFGIDNINFPGDRTLFLYWNDGKINLVQF
                     MVEQVIPVTVILVLSIVLVAVLWALRWLRSVRDLMIVLFSGFMAVYVTLTVVGTAFRG
                     EGQELVPPWDVPRHPEGA"
     gene            2656..3483
                     /locus_tag="VNL92_01480"
     CDS             2656..3483
                     /locus_tag="VNL92_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWO93414.1"
                     /translation="MTDEQERQPDESSESPSEAAPSDETPADEVTEAAQTEGTERAAA
                     REAARTPRAQAARSEAEGAAEEEPAAAAEPQTPVARVEDRIVTRKTRERTGEQREVTR
                     RAVLRTGFFGTMGAIAALGAYQLWNMLWPRGLTGFGGVINVSAERVPQAGADPVRIIE
                     GKFWLVNLQADEGTFGQFGQAGQGGVLALYQKCPHLGCTVPWRPTFVFEGQEGWFRCP
                     CHGSTYTKAGVRVFGPAPRPMDTMAVTVNGDGSVSVDTGAITLGDVDNPQRAVAYNA"
     gene            3529..4722
                     /locus_tag="VNL92_01485"
     CDS             3529..4722
                     /locus_tag="VNL92_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWO93415.1"
                     /translation="MNTTKQIQIMVGLVLLLVVGLIAYSIWEPERQEDAEATQLEDQV
                     ERGAELFASNCTTCHGLEGEGFVGPALNVPANRPEDPAELQRLQDRFTNTIRCGRVGT
                     FMPAWAQDQGGSLNNTQIEQLVLLITVGGEIDGQSEWEFVREHAEELNVEVTQPTPDQ
                     LNQGACGQVLRETPTPEPEGPVEVRTEWDISMGDNFFEPNRIGVPVGQQVTVNLTNDG
                     QAIHNMRIAGPDGEYDTDDDVVSDPDTVRPGEEATLTFTANEDGTIDFRCDFHPTEMT
                     GTIVVGEEAQAAGGAAPTPSAQAEAEWDVTMADNSFDPDEIVVPAGQEFTIALTNEGQ
                     AVHNMRIAGADGEYDTDDDIVSEPDTIRPGEDGELMGSIAQAGTMEFRCDFHPVEMIG
                     TLTIQ"
     gene            4789..5310
                     /locus_tag="VNL92_01490"
     CDS             4789..5310
                     /locus_tag="VNL92_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWO93416.1"
                     /translation="MAFLRRRGHLALFLLVALLGLALVAAACGDDDDDDDDDDGTATV
                     EVTETEEAGGATNATVTETATEEAETPSAETPSDGATETTFEVIMGDNFFEPNEFTVP
                     AGEEITFELVNEGDAIHNMAVEVGGENFVSEPDSIRGGEEGTLTVTFDEPGEYDFLCE
                     FHPTEMTGTITVE"
     gene            5433..5726
                     /locus_tag="VNL92_01495"
     CDS             5433..5726
                     /locus_tag="VNL92_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93417.1"
                     /translation="MAAQVGKRYKAASGAEFIVTKGGNGTLMDGDAELVLSDSGQAPQ
                     PGPSDAADVQLGKRYKSADGSVEVLVIKPGKVDLRCNGEPMELMQPKVLPSAD"
     gene            5784..5860
                     /locus_tag="VNL92_01500"
     tRNA            5784..5860
                     /locus_tag="VNL92_01500"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5818..5820,aa:Pro,seq:cgg)
     gene            complement(5915..7057)
                     /locus_tag="VNL92_01505"
     CDS             complement(5915..7057)
                     /locus_tag="VNL92_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWO93418.1"
                     /translation="MMVASEQPKPRRPRGEGSIKRVGDRWLVRLRAGGRDERRWFATQ
                     GEALDELDRLRRKAALGTRGRYTVADCIDDFLAQAEARQWSPSTVATYTDVLRLHIKP
                     ALGKRQLAELRVHDVQRVLDRMREHGLSARYVSHTRSVFRAAVNHAMRAEHVHRNVAA
                     LTSAPAGRQAETHVLSREEMGRLFAALEGERLRTMIMVMATLALRRGEAVALRWEDLA
                     LDDGLLTVRRTAKRVGREHYEGEPKTARSRRTLALPPFLVAALRAQRAAVVAEQLRLG
                     PAWRDEGYVFPGEDGGPCSANAIRLALSRGLKRAGIEKHVRIHDLRHSAISALLAGGG
                     SLQDAQALAGHTSISLTADLYAHFMPDQRAATAARIEAYLGSAVNP"
     gene            complement(7041..7661)
                     /locus_tag="VNL92_01510"
     CDS             complement(7041..7661)
                     /locus_tag="VNL92_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93419.1"
                     /translation="MPTKTSSWWRNQTFNLFRDFEAQGKRLVATDIARQLEGIARRDS
                     TPPEERVRYPAERTVRTLRQEYEKLPREDQTQFGWVRWPDSFEDGSLPWEAAPAVLEL
                     LRLKAGQLAELRYEWPFAGAGGHWEAGRPDVRLAAAFWRVTQAAPRHCVECRLSVAEL
                     LEQDSWLARRIAEERLLDKPECPLGDLPGGHVLGTRTKEEPNDGGK"
BASE COUNT         1407 a         2516 c         2516 g         1366 t
ORIGIN      
        1 gacttcgcgt ggcgcatcgt cgtcctgaac aacggctcca ccgacgacac gctggagcgc
       61 gcgtgccgca ttgcggaatc gtgcagagag gtcggggtgc ttcacttacc cgaaaagggg
      121 cgtggccgag cccttcgcca cgcgttccag caatcggacg ccgacgttcg gatgtacatg
      181 gacatcgatc tgtccacgag cctcgatgcg gtgcttccgc tcgtgcgggc catcacggag
      241 gaggggtacg acttggccgt gggcacgcgg ctgcatcgcg atgcagctgt tgcacgctcc
      301 ctggagcgcg aagccgtctc ccagatgtac aacctactcg tgcgcttcgt gttcgacacg
      361 cggttcagcg acgcccaatg tgggttcaag gctgtctctg ccaagacggc cagggtcctg
      421 gtgccgcaag tcaaggacga cgagtggttc ttcgatacag agctgctgct cctcgcggag
      481 aggaatggct accgtatcaa ggatgtcccc gtcgtctggg tcgaggaccc cgacagtcgg
      541 gtgaacgtag ggcgcacagc ccttcaggac gtgcgcggca tggcccgtct gaagttcgga
      601 ggcatcccac gcatcattgc ggcggagtgc ggacgggagg ggcgcactcg tgcgttcggc
      661 agttgacagt ggtcccgcac gatgctagat ttccgtctgc ttgcaaacgc cttcacaaag
      721 cctgccggat gccgcggtcg ctccttgcat ccggtcggga ccaggggtaa ggtcgaaggg
      781 ccgcagctac tttcgaccat catccgcaac cggaggagtc gatacatgtt cggagcggtc
      841 ggcgagacgc tagagctcgt tgctgtgttc atcacgatcc tcggttccct cggcattatg
      901 gtcgccttcg ggcagtggtg gcagcgctga tcgcgggaga agctccatgg cggtaaccga
      961 acaaccagcg aagccgagtt tccgcgagcg agtgtacgat cgtctgttcc acaactacat
     1021 ctggcggtcg atcttccgct ccggctaccc gaacactccg cgcaaccaga tgctcgtcgt
     1081 cgcgacgaac gtcttcctcc accttcaccc gacccgcatt caccgcaccc acgtcaagat
     1141 cacacacacg tactgtctgg ggggcctgag cttcttcatg ttcctcgggc tcacggtgac
     1201 cggcgtgctc ctgatgttct actacattcc atcgaccgag caggcgtacg cgaacatgca
     1261 ggccctggag acggacgtgc agtttggcat gctcatccgc aacctgcatc gatggatggc
     1321 gcacgcgatg gtcatcctcg tcttcctgca catgatgcgt gtgttctaca ccggtgcgta
     1381 caagccgccg cgcgagttca actgggtggt gggcgtggtc ctgctggtgc tcacgcttct
     1441 gctgagcttc accggctacc ttctgccctg ggaccagctg tcgatctggg ccatcacggt
     1501 gggcagcgag atcgccggct cggccccggt gctcggcgac gacgtgaagt tcacgctgtt
     1561 cggcggtttc agcatcggcc agacctcgct gatcaggttc tacacgttgc acgtcatcgc
     1621 gctcccgctg ctggcggccg tgtttatggc cgtgcacttc tggcgcattc gccgcgatgg
     1681 cgggatggcg aggccgctct agggcgcaga cgccagtaca aaggaagaga gaggcatggc
     1741 ggagcagacg gctactcgac cgcggccgcg ccgcgaacgc gaggaggaag tcctcgtatg
     1801 gcctgatctc gtcttcgtcg agttcatcgc cgcagtcctg ttcactatca cgctcattct
     1861 gctttccacc cttgtcaacg cgccgttgct cgacagggcg aaccccgacg ttacgcccaa
     1921 cccatcgaag gcgccatggt acctgctgaa cctgcaggag ttgctgttgc acatggaacc
     1981 gggcctcgcc ggcgtcgtca ttccgaccgt ggccctcgtg ggacttgccg ccattccgta
     2041 tatcgaccgc aaggaggaag ggcggggagt ctggttcgcc actccgaacg ccgtgaagct
     2101 cacgatcttc gcctgggtct acactgtcgt gctcacggtc gtgctcatcc tcttcgatgc
     2161 cggcaagttc gagtcctaca ccgaatggtt ccctggcctt cccgatgggc aaggactatc
     2221 gggcacacgt acgctgcaga cgagctggtc gtggagcatc ggcgacttcg actatccgga
     2281 ggacctggag gctgtcggcg cgccctttgg tatcgacaac atcaacttcc cgggcgatag
     2341 aaccctgttc ctctactgga atgacggtaa gatcaatctt gtgcagttca tggtggagca
     2401 ggttatcccc gtcacagtga tcctcgtgct ttcaatcgtg cttgtcgccg tgctttgggc
     2461 attacgatgg ttgcggtccg tgcgcgacct gatgatcgtg ctcttcagcg ggttcatggc
     2521 ggtctacgta accctgacgg tcgtgggcac ggccttccgc ggcgagggac aagagctcgt
     2581 tccaccgtgg gacgttccgc gccacccaga gggcgcgtaa ccccgacatt gtttcggcct
     2641 cgaggatgag tgatcgtgac tgacgaacaa gagcgacaac cagacgaatc ttccgaatcg
     2701 ccgtccgaag cggcgccttc ggacgagaca ccagcggacg aagtgacgga ggccgcccag
     2761 accgaaggca cggaacgtgc cgcggcacgg gaggcagccc gcactccgcg cgcccaagcg
     2821 gctcgaagcg aggctgaggg cgctgcagag gaggagcctg cggccgccgc cgaaccacag
     2881 acgccggttg ctcgcgtcga ggatcgcatc gtcacgcgaa agacacgcga gcggaccggg
     2941 gaacagcgcg aagtcacgcg ccgggcggtc ctccgcaccg ggttcttcgg gaccatgggg
     3001 gccatagccg cccttggcgc atatcagctc tggaatatgc tgtggccgag agggctcacc
     3061 ggcttcggcg gcgttatcaa cgtgtcggcc gagcgagttc cgcaggccgg ggccgatccc
     3121 gtgcgcatca tcgagggcaa gttctggctt gtgaacctcc aagcagacga agggacgttc
     3181 gggcaattcg gacaggcggg ccagggcggc gtcctggccc tgtaccagaa gtgcccccat
     3241 ctgggatgca cggttccctg gcgccccaca ttcgttttcg aggggcagga gggatggttt
     3301 cgctgcccct gccatggctc cacgtacacg aaggccggcg tccgcgtctt tggaccggct
     3361 cctcgaccca tggacacgat ggccgtgacc gtcaatggcg atggcagcgt gtcggtcgat
     3421 acgggagcca tcacactcgg ggacgtcgat aatccccagc gcgctgtagc ctacaacgcc
     3481 taaacgtccc ctgtcgggca gggggaagag gagagtgccg gagactcgat gaatacgacc
     3541 aagcagatcc agatcatggt tgggctggtg cttctgctgg tcgttggcct gatcgcctat
     3601 tcgatatggg agccggagcg tcaggaggac gccgaggcca cacagctgga agaccaagtc
     3661 gagcgcggcg cggagctctt cgcgagcaac tgcacgacct gccacgggct ggagggggaa
     3721 ggcttcgtcg gccccgcgct caatgtgccc gcgaaccgtc ctgaagaccc ggccgagcta
     3781 cagcggctac aggaccgttt cacgaacacg atccgctgtg gacgcgtcgg gacgttcatg
     3841 cctgcttggg cccaggacca gggtggctcg ctcaacaaca cccagatcga gcaactcgtc
     3901 ctgctcatta cggtgggcgg ggagatcgac gggcaatccg aatgggaatt cgtccgtgag
     3961 cacgccgagg agctcaacgt cgaggtgacg caacccaccc cggaccagct caaccagggg
     4021 gcctgcggac aggtactgcg cgagaccccg acgccggagc cggaggggcc ggtcgaggtg
     4081 cgcaccgagt gggacatctc gatgggcgac aacttcttcg agccgaaccg catcggcgtg
     4141 cccgtcggac aacaggtgac ggtaaacctt accaacgacg gtcaagcaat ccacaacatg
     4201 cgcattgcag gtcccgacgg ggagtacgac acggacgacg acgttgtctc ggaccccgac
     4261 accgttcgac ccggcgaaga ggccacgctg acgttcacgg caaacgagga cggaacgatc
     4321 gacttccgct gcgacttcca ccctacggag atgacgggta cgattgttgt aggtgaagaa
     4381 gcgcaggctg cgggcggcgc cgcgccgact ccaagtgccc aagcagaagc cgagtgggac
     4441 gtgaccatgg ccgacaactc gttcgatccg gacgagatcg tcgtcccggc gggacaggag
     4501 ttcacgatcg ctctcacgaa cgaagggcag gccgtgcaca acatgcgcat cgcaggtgca
     4561 gacggcgaat acgacaccga cgatgacatc gtctccgagc ccgacaccat tcgccccggc
     4621 gaggacgggg aactcatggg aagcatcgca caggcgggca caatggagtt tcgctgcgac
     4681 ttccaccctg tcgaaatgat aggcaccttg accatccaat agctcacttc gccccgtaac
     4741 tatcgcaaag ctatcgcccg ttgagggcgt gaaaggagtg gtatgctcat ggcatttttg
     4801 aggaggcggg gtcatctcgc attgttcctg ctggtggcgt tgctcggctt ggcgctggtc
     4861 gccgccgcct gcggagatga tgacgacgat gacgacgatg acgacggcac ggctaccgtc
     4921 gaagtgaccg aaacggagga ggccggcggc gcgacgaacg ccaccgtcac cgagactgcg
     4981 accgaggagg cggagacgcc gtccgcagag acgccctcgg atggcgcaac cgagacaacg
     5041 ttcgaggtca tcatggggga caacttcttc gaacccaacg agttcaccgt gccggccggc
     5101 gaggagatta cgttcgaact cgtgaacgaa ggcgacgcca tccacaacat ggccgtcgag
     5161 gttgggggcg agaacttcgt gtccgaaccc gactcgattc gcggtggtga agaaggtacg
     5221 ctgaccgtca cgttcgacga gcccggcgag tacgacttcc tctgcgagtt ccaccccacg
     5281 gagatgacgg gtacgatcac cgtcgaatag gtgtcaaagg cgcacaagag ttgacgagtt
     5341 gcgggccccg gtcatagact gggcccgcac tcacgtccca aggcacatca attcctagac
     5401 gcgcctcgcg cgcgcatcga ggtgatgaat acgtggcagc ccaagtcgga aagcgataca
     5461 aggccgcgag cggcgcagag tttatcgtca ccaagggcgg gaacggcacc ctgatggacg
     5521 gcgatgctga gctggtcctc tccgattcag gccaggcgcc tcagccgggt ccgagcgacg
     5581 cagcggacgt acagctcggc aagcgataca agagcgccga cgggtccgtc gaggtcctcg
     5641 tgatcaagcc cggcaaggtc gacctgcgct gcaatggcga gcctatggag cttatgcagc
     5701 ccaaggtgct tccctcggcc gactgaccgc ggatagaatg atagtgaaga tcgcccgtct
     5761 ccgtggccgg gcgatggctc cggcggggtg tggcgcagcc cggtagcgcg cttcgttcgg
     5821 gacgaagagg cccccagttc aaatctgggc accccgacca actttcagat tcaatttcag
     5881 cccatttggc ccctcggctg cccttacact gcccttacgg gttcactgcg gagccgagat
     5941 acgcctcgat gcgggcggcc gtggccgctc gctggtcggg catgaagtgg gcgtagaggt
     6001 ctgctgtcag cgagatcgac gtgtgcccgg cgagcgcctg ggcatcctgc aggctcccgc
     6061 cgcccgcgag cagggccgag atcgcggagt gccgcaggtc gtggatgcgg acgtgcttct
     6121 cgatgcccgc gcgcttcagt ccccgcgaaa gcgcaagccg gatagcgttg gccgagcacg
     6181 gcccgccatc ctcgccgggg aacacatatc cctcgtcccg ccacgccggc cccagcctga
     6241 gctgttcggc cacgacggcg gcacgctggg cgcgcagtgc cgccacgagg aacgggggaa
     6301 gcgccaaagt ccgtcgtgac cgggccgtct tcggctcgcc ttcgtagtgc tcacggccga
     6361 cgcgcttcgc cgtccgccgg acggtgagca gcccgtcatc gagtgcgagg tcttcccagc
     6421 gcagcgcgac cgcctcgccg cgccgaagcg cgagcgtcgc catcaccatg atcatcgtcc
     6481 gcaatcgctc gccttcgagc gctgcgaaga ggcggcccat ctcttcacga gacagcacgt
     6541 gtgtctctgc ctgccgcccg gcgggcgcac tcgtgagggc cgcgacgttt ctgtgcacat
     6601 gctccgcgcg catcgcgtga ttgaccgccg cccgaaacac gctccgggtg tgcgagacgt
     6661 agcgggccga caggccatgc tctcgcatcc tgtcgagcac gcgctgaaca tcgtgcacgc
     6721 ggagctccgc gagttgacgc ttgccaaggg cgggcttgat gtgcagacgg agcacgtccg
     6781 tgtacgtggc gaccgtcgat ggcgaccact gccgcgcctc ggcttgagcg agaaagtcgt
     6841 cgatacagtc cgccacggtg tagcgtcccc gcgtaccgag cgccgccttg cgccgcagcc
     6901 ggtcgagctc gtcgagcgcc tccccctggg ttgcgaacca ccgccgctcg tcccggccgc
     6961 ccgcgcgtag gcggacgagc caccggtcgc cgacgcgctt gatcgagccc tcgccgcgcg
     7021 gccgtcgcgg ctttggttgt tcacttgcca ccatcattag gctcctcctt cgttctggtc
     7081 cccaggacgt ggccgcccgg cagatcgccc agcggacact ccggcttgtc caggaggcgc
     7141 tcctcggcta tccgccgggc cagccatgaa tcctgttcca ggagctccgc gacggacagc
     7201 ctgcactcca cgcagtggcg gggcgcggct tgcgtcacgc gccagaacgc tgctgcgagg
     7261 cgaacgtcgg gacggccggc ctcccagtgc ccccctgctc ctgcgaaggg ccactcgtac
     7321 cgcaattcgg cgagctggcc ggccttcagg cgcagcagtt cgaggacagc gggtgccgct
     7381 tcccagggta gggaaccatc ctcgaagctg tcgggccaac ggacccaccc aaactgcgtc
     7441 tggtcctccc gcggcagctt ctcgtactcc tggcgaagcg tccgcacggt tcgctcagct
     7501 gggtagcgca cccgctcctc aggcggggtc gagtcccgcc gtgcgatgcc ctccagttgc
     7561 cgcgcgatat cggtcgcgac cagccgcttg ccctgtgctt cgaagtcgcg gaacagattg
     7621 aaggtctggt tgcgccacca cgagctcgtc ttcgtaggca ttcgccctcc aattagccgg
     7681 tttgcccctt tttgccgccg cacggtccgg ctcgttagcc gtatgctcag catagcaagc
     7741 cgcgtcatgc agtatcgcac gaaccgcggt gaacttcaag cgaggaggtg gttttgaaat
     7801 ggagg
//
LOCUS       DATMBB010000071         3177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_226007, whole genome shotgun sequence.
ACCESSION   DATMBB010000071 DATMBB010000000
VERSION     DATMBB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226007"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VNL92_01515"
     CDS             complement(<1..1178)
                     /locus_tag="VNL92_01515"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93420.1"
                     /translation="MLFEGVKVLDFSWGAALPLATKIMAHYGATVVSVESQRRPTLTR
                     LLPPYHGGMPGLEQSAMFANFGTSKLSIALDMNHPKGQQLARRLATEWAGIVAESFGP
                     GIMTRWGMTYDQLREVKPDLFMVSSSMFGQEGPYASYRGYGSHGASVAGFATITAWPG
                     EVPAGPYGAYTDFISPRLTAAALLAAWDHHERTGESVYLDYSQVEGAVQFLGFAALDY
                     QANGRVLGPARNRTAEGAPHGVYPCIGDDRWVAITVFGDEQWQRFCQAIGRPELASDP
                     RFATHGQRKANEDALDAEVAAWTRDREAHGAAALLQAAGIAAGAVQDCRDLRNDPHLR
                     AWGFYEQRQHPVFGDFEYDGLEFRIQDVEPTFERSPLLGEHTHMVLHDFLGLADHEIA
                     "
     gene            complement(1182..2516)
                     /locus_tag="VNL92_01520"
     CDS             complement(1182..2516)
                     /locus_tag="VNL92_01520"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93421.1"
                     /translation="MVRTSAERPLLPPLRVLDLTTELGAFAGKVLGDLGADVVKIEPP
                     GGDPDRMLGPFWNDEPNPNRSLRWWSANTSKRSAVVDIGQPEGRETFLRLVRSADFVL
                     ESFPPGQMASHGLGWDDLARANPRLILVSMTPYGQVGPRANEPVSDLVLMSMGGMTYL
                     CGDEGHPPVTVAVPQSYYHVGAEGAEAALIAYFARKRTGQGQWVDVNGQACIAWTLLS
                     EIPMADATFHNAPSRRSGPVRERGVLRSRTVFPCADGYVNFLVAGGSAFAGTIRHLIA
                     WMDSEGEAPDFLKEIDWMTWSIEAFAFLHPPEEHPAIQQQVERVEGAFAAFFARRTKE
                     ELLAGAAQWQVMLGPLLTIDDLLVNRQLQSSGYWVPVEHEDIGVTVRYPGVLARSNVG
                     GGMIRRRPPNIGEHTFEVLEEYGLGRDEIEHLAAAGVTSIPVGAQGHRESGR"
     gene            2690..>3177
                     /locus_tag="VNL92_01525"
     CDS             2690..>3177
                     /locus_tag="VNL92_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO93422.1"
                     /translation="MDVLHKDPLLEVYRLRLGPYDNNAYVIIDPEARRAAIIDAPPEG
                     EAILDVVRPHAVDHILITHEHPDHIMTLGLLRDATGAKVHAHEDETNVPGELIDARLR
                     HGDVLQVGRHEIRVVHTPGHTPGSVSFVLGDYLFSGDTLFPGGPGASRSPEALRQEIR
                     SIT"
BASE COUNT          529 a         1132 c         1011 g          505 t
ORIGIN      
        1 tcggcgatct cgtggtcggc gaggccaagg aaatcgtgca gcaccatgtg cgtatgctcg
       61 ccgagcaggg gcgagcgctc gaacgtgggt tcgacgtctt ggatgcgaaa ctcgaggccg
      121 tcgtactcga aatcgccgaa cacggggtgc tgccgctgct cgtagaaccc ccaggcgcgc
      181 aagtgcgggt cgttccgcag gtcccggcag tcctgcaccg cccccgcagc gatgccggcc
      241 gcctggagga gcgccgccgc cccgtgcgct tcgcggtcgc gcgtccaggc cgccacctcc
      301 gcgtccaaag catcctcgtt cgccttgcgc tggccatggg tggcaaagcg tgggtcgctc
      361 gccagctcgg gccggccgat cgcctggcaa aacctctgcc attgctcgtc cccgaagacc
      421 gtgatggcga cccagcggtc gtccccgatg cacggatata cgccgtgcgg cgcgccttcc
      481 gccgttcggt tgcgggccgg gcccagcacg cggccgttcg cctggtagtc caaggcagcg
      541 aagcccagga actgcaccgc cccctccacc tgggagtaat cgaggtagac cgactcgccc
      601 gtgcgctcgt ggtggtccca ggctgcgagc agcgcggccg ccgtcaatcg tggcgagatg
      661 aagtcggtgt acgctccgta ggggccggcc ggtacctcgc cgggccacgc cgtgatggtc
      721 gcgaagccgg cgacggacgc cccatgggag ccgtagccgc gatacgacgc gtaaggaccc
      781 tcctgcccga acatgctgct gctcaccatg aacagatccg gcttaacctc acgtagctgg
      841 tcgtaggtca tgccccatcg ggtcatgatg cccggcccga agctctcggc gacgatgccc
      901 gcccactccg tcgccaatcg ccgggccagc tgttgtccct tcggatggtt catgtcgaga
      961 gcgatgctga gcttgctggt cccgaagttg gcgaacatcg cgctctgctc caacccgggc
     1021 atcccgccgt ggtagggagg cagcaggcgg gtcagcgtgg gccgccgctg cgactcgacc
     1081 gatacgacgg tcgcgccgta gtgagccatg atcttggtcg ccaggggcag ggcagcgccc
     1141 cagctgaaat cgagaacctt cacgccttcg aacagcatcg atcacctccc gctctcgcga
     1201 tgcccctggg ccccaaccgg aatggacgtg acgcccgccg cggcaagatg ctcgatctcg
     1261 tcccggccga gtccgtactc ttcgagcacc tcgaacgtgt gctcaccgat gttaggtggc
     1321 cgcctccgga tcatcccgcc acccacattt gagcgggcga gcacgcccgg gtagcgcacg
     1381 gtgacgccga tgtcctcgtg ctccaccggg acccagtagc ccgatgactg cagctgccgg
     1441 ttcacgagca gatcgtcgat ggtgagcagc gggcccagca tcacctgcca ctgagcggcg
     1501 cccgccagca gctcctcttt cgtacggcgg gcgaagaagg cggcgaacgc cccctccact
     1561 cgctccacct gctgctgaat cgcgggatgc tcttcgggcg ggtggagaaa tgcaaacgcc
     1621 tcgatgctcc acgtcatcca gtcgatctcc ttgagaaagt ccggcgcttc gccctcgcta
     1681 tccatccagg caatcagatg gcgtatcgtg ccggcgaagg cggagcctcc tgcaaccagg
     1741 aaattgacgt agccatcggc gcaaggaaac acggtgcgcg agcggaggac gccgcgctcg
     1801 cgcacgggac cgctccgacg agagggagca ttgtggaagg ttgcgtcggc catcgggatc
     1861 tcggagagta gcgtccaggc gatgcacgcc tgcccgttca cgtccaccca ctgcccctgg
     1921 cccgtgcgct tccgtgcgaa gtacgcgatg agcgccgcct cggcgccttc cgccccgacg
     1981 tggtagtacg attggggcac ggcgaccgtc accggcgggt gaccctcgtc gccgcagagg
     2041 taggtcatgc cgcccatcga catgagcacg aggtcggaca ccggctcgtt ggctcgcggc
     2101 cccacctgtc cgtacggcgt catggagacg aggatgagcc ggggattggc ccgcgcgagg
     2161 tcgtcccagc cgaggccgtg cgacgccatc tgcccgggcg ggaacgattc gagcacgaaa
     2221 tcggcgctcc gcaccaggcg caggaacgtc tcgcggccct caggctggcc gatgtcgacc
     2281 acggcgctgc gcttgctcgt gttcgccgac caccagcgca gcgagcggtt cgggtttggc
     2341 tcgtcgttcc aaaacggccc gagcatccgg tcggggtcgc cgccgggcgg ctcgatcttc
     2401 acgacgtcgg cgccgagatc gcccagcacc ttgccggcaa aggcgccgag ctctgtggtc
     2461 aggtcgagca cccgaagagg aggcagcaga ggccgttcgg ccgaggtacg gaccatggat
     2521 actcctcact tccgtttcga tggcttccct gacattagcc acatcccgac gcttgtcaag
     2581 ggcccgagtc gtccctccac gcaaacgatg cccttttgac acgccggaag ccctctgcta
     2641 tcctcacgcc ccgaagcaag cccaccgacg ccgaatcggg agcacatgca tggacgtcct
     2701 ccacaaggac ccgctcctcg aggtctatcg cctccgtctc ggcccgtacg acaacaacgc
     2761 gtacgtcatc atcgatccgg aggctcggcg ggccgccatc atcgacgccc cgcccgaggg
     2821 cgaggcgatc ctcgacgtgg ttcgcccgca cgccgtcgac cacattctca tcacgcacga
     2881 gcatccggat cacatcatga cgctgggcct cctccgcgac gcaacgggag cgaaggtcca
     2941 cgcgcacgag gacgagacca acgtcccggg cgagctcatc gatgcccggc tacgacacgg
     3001 cgatgtcctc caggttggca ggcacgaaat ccgcgtcgtc cacacgccgg ggcatacccc
     3061 tggcagcgta tcgttcgtct tgggcgacta cctgttctcc ggcgacacgc tcttccccgg
     3121 cggccccggg gcctcgcgtt cgcccgaggc gctccggcag gagatacgct ccatcac
//
LOCUS       DATMBB010000072         2249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_497659, whole genome shotgun sequence.
ACCESSION   DATMBB010000072 DATMBB010000000
VERSION     DATMBB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497659"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /gene="panC"
                     /locus_tag="VNL92_01530"
     CDS             complement(<1..574)
                     /gene="panC"
                     /locus_tag="VNL92_01530"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWO93423.1"
                     /translation="MDLLRTVAEARSWRGQTPRPVGFVPTMGYLHEGHLSLIRRAREE
                     NPVVAVSIFVNPTQFGPNEDLARYPRDEHRDLSLLAEAGLDAVFVPSAEEMYGPAFST
                     WVDVTGPLTTRLEGAHRPGHFRGVATVVAKLFNILAPDRAYFGQKDAQQLAVVRRMTR
                     DLNLPVEIVACPTVREYDGLAMSSRNVYLSP"
     gene            complement(583..1416)
                     /gene="panB"
                     /locus_tag="VNL92_01535"
     CDS             complement(583..1416)
                     /gene="panB"
                     /locus_tag="VNL92_01535"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWO93424.1"
                     /translation="MARKTITDMQAMKHRGEPIVMLTAYDFPSARLVDDVADIILVGD
                     TLGMVVLGYDSTLPVTVDDMIHHTKAVVRGSERALVVGDMPFMSYQTGPEDALRNAAR
                     FLQEAGATAVKLEGGARSAPVVQRLTEAGIPVMGHIGLTPQSVNQFGGYKAQGKTRQQ
                     ALALLRDAKALQDAGAFAIVLEVIPAPLAKLVTERVDIPTIGIGAGPDCDGQVQVYHD
                     LLAYATTFVPRHAKQYARIGDDILAALHRYADEVRSGAFPTPGHSVGMDDDLARELAE
                     A"
     gene            complement(1515..1907)
                     /gene="panD"
                     /locus_tag="VNL92_01540"
     CDS             complement(1515..1907)
                     /gene="panD"
                     /locus_tag="VNL92_01540"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HWO93425.1"
                     /translation="MRTMLRGKIHRARVTEANPDYEGSVTIDADLMEAAGILPFEQVH
                     ILDITNGQRLQTYAIEGERGSGEVCVNGAAARLVAKDDLVIILAYGLMGDDEARRHEP
                     QLAYVDASDGNRLIRVAHQVEPRTVFAV"
BASE COUNT          382 a          739 c          725 g          403 t
ORIGIN      
        1 gcggggacag atagacgttg cggctgctca tcgccaagcc gtcgtattca cggacggttg
       61 ggcaagcaac gatctcgacc ggaagattga ggtcgcgggt catccggcgg acgacagcga
      121 gctgctgcgc gtccttctgc ccgaagtagg cccgatcggg cgcgaggatg ttgaacagct
      181 tcgcgacgac cgtggccacg cctcggaagt gccccggcct gtgtgcgcct tccagcctgg
      241 tcgttagcgg cccagtcaca tcgacccacg tggagaaggc ggggccatac atctcctcgg
      301 ccgaaggcac gaataccgca tcgaggcccg cctccgccag cagcgagagg tcccgatgct
      361 catcgcgcgg atagcgtgcg aggtcctcgt ttggcccgaa ctgggttggg ttcacgaaga
      421 tgctcacggc gacaaccgga ttctcttcgc gggcgcgccg gatcagcgaa aggtggcctt
      481 cgtgcaggta ccccatcgtc ggaacgaagc cgaccggccg cggagtttgg ccgcgccagg
      541 agcgcgcttc agcgacggtg cggagaaggt ccacgaccgg cgttacgcct ccgccaactc
      601 ccgggcaagg tcgtcgtcca tgccgacgct gtgtccgggc gtggggaagg cgcccgaccg
      661 aacctcgtcg gcgtaccgat gcaacgccgc aaggatgtcg tcgccaatgc gcgcgtattg
      721 cttggcgtgg cgcggcacaa acgtcgtcgc gtaggcgagc agatcgtggt acacctgcac
      781 ctgcccatcg caatccggcc cggcgccgat gccgatggtt gggatgtcga ctcgctcagt
      841 gaccagcttc gcgagcggcg cggggatgac ttcgagcacg atcgcgaacg cgcctgcatc
      901 ctgcagagcc ttggcgtcgc ggagcaacgc gagcgcctgc tgccgcgttt tgccctgcgc
      961 cttgtagccc ccgaactgat tcaccgattg tggcgtcagg ccgatgtgtc ccatgacggg
     1021 gatgccggcc tcggtcaatc gctggacgac gggagcggaa cgagctcctc cctccaactt
     1081 gaccgccgtt gcgccagcct cctgaaggaa gcgggccgca ttgcggagcg catcctccgg
     1141 acctgtctgg tacgacatga agggcatgtc gccgaccacg agcgctcgct cgctgccccg
     1201 tacgacggcc ttcgtgtggt ggatcatgtc gtcgacggtg accggcaagg tcgagtcgta
     1261 gccgagcacg accatgccga gagtgtcgcc gaccaagatg atgtccgcca cgtcgtccac
     1321 gagccgtgcc gagggaaaat cgtacgcggt gagcatgacg atcggctcgc cacgatgctt
     1381 cattgcttgc atgtcggtga ttgtcttgcg ggccattgcc tgacctccag atgaacagcg
     1441 gggcatatgc caagggcgct cgcggtgttt ctgcccgtgc acgccggaag ggctggacgg
     1501 agagagttgg gaatctagac ggcgaagacc gtccgaggct ccacttggtg ggccacccgg
     1561 atgagccggt tgccgtccga ggcgtcaacg tacgccagct gcggctcgtg gcggcgggcc
     1621 tcgtcgtcgc ccatgagacc gtacgcgagg atgatgacga gatcatcctt cgcaacgagg
     1681 cgcgccgctg ccccgttcac gcaaacctcg ccgctgccgc gctccccctc gatggcgtac
     1741 gtctgcaacc gctggccgtt cgttatgtcg agtatatgca cctgttcgaa cggcagaatg
     1801 cccgcggcct ccatgaggtc ggcgtcgatg gtgacgctcc cctcgtagtc cgggttcgct
     1861 tccgtgacgc gtgcccggtg aatcttcccc cgcagcatgg ttcgcatacc aatctccctc
     1921 gtcggcgtcc atgcggatcg ccgctcattc gatacgcgga cgaaaaagcc cccctgacct
     1981 acattcggcc gggaggcgtt cgtgcggctt ttcggctatc ccctactcgt ctcggctggt
     2041 agttaccaat ccaagcgacg ggagtgccgc tccgacacat actccgctcc ccgcccattg
     2101 ggtcgaaggc cacatgagta ttaacgccgc tcaacgggct gtcaacccgt ctcgcgaagt
     2161 atttgagaac tatcgtgaat ctgcgcctag acggcgccat tcctcggcgg aaggtagtgg
     2221 cggacgacct tccgccgccc gcacgccct
//
LOCUS       DATMBB010000073         5458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_78773, whole genome shotgun sequence.
ACCESSION   DATMBB010000073 DATMBB010000000
VERSION     DATMBB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78773"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1561)
                     /locus_tag="VNL92_01545"
     CDS             complement(104..1561)
                     /locus_tag="VNL92_01545"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWO93426.1"
                     /translation="MTSFVVFFPLLGALVVALTPSSAPIRPRVTAAVTTFVVFVATVV
                     MFVLFDRDDGGFQFEQEFTWIDADNFEIKYLMGVDGISVTLLLLTGLLTFIAVLISWG
                     IETRAREYFVWLLVLETGVLGVFTSLDLVLFFLFWEVELIPMYLLISIWGTGRKEYSA
                     IKFLAYTLFGSGFMLAAILALGFSQGTFDYTELSQMTIADTEIPVELIFFGFFAAFAI
                     KLPVFPVHTWLPDAHTDAPTAVSVMLAGVLLKMGGYGIIRFCIGLMPDVARDWDVLLA
                     ALAATSVVYGALVTLRQTDLKRLIAYSSVSHMGFVLLGVAALGSTSLSGATLQMFTHG
                     TITGLLFLMVGVIYDRTHTRQIPEMRGLASRMPVAALVTLIAGFASLGLPALSGFAAE
                     LTVFLGSFGRHEAATIAVATGVVLAAGYILWTIERVFHGPVDEERWGHLTDADQWWER
                     VPMAALVVAIVVVGVYPAVLTDVIEMGIEPIAGRL"
     gene            complement(1563..3518)
                     /gene="nuoL"
                     /locus_tag="VNL92_01550"
     CDS             complement(1563..3518)
                     /gene="nuoL"
                     /locus_tag="VNL92_01550"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWO93427.1"
                     /translation="MFDTINEAAVWAIFFLPVASLALIAVQWALRTPDEFAALTIIAA
                     LVGSFGLALWALDSGIDADGHIIGFGTHEWLSLGDLTVNIGIRLDGLTSVMLVVVSAV
                     SLLVQVYSVGYMHGDPGYKRYFAVMSLFTAAMLGLVLADNLIMLFVFWELVGASSYLL
                     IGFWFRRPSAAAAAKKAFIVTRLGDLGFLLAILLIYREIGTFDIEAIHEAALAGAIGS
                     AMLSWFAVGVFAGAAGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVARLF
                     PVFSVSDEAMTTVAVIGSITALGAALLGVVMFDIKRVLAYSTISQLGYMMVALGVGAY
                     VAAIFHLMTHAFFKALLFLGSGSVNHATNTFDMRRMGGLARAMPVTAATFVIGALSLS
                     GIPPFAGFWSKDEVLAGAFDENWAYFLVLLGAAFLTAFYMFRAIFMTFAGEYRGGEPA
                     AADGHAGGDAEEHAAEAHEAHPHESPWVMALPLVVLAVPAVLVGFWNIDHDFTHLVEG
                     ALPEGVEVHEFEFEWPIATASTGVALLGVFTAAAIYYEPLRVMSAEWLGARLRPAWTL
                     LSNRYYLDALYETVVVRWLFYAGLVRALALFDQYVVDGIVNGAARTGDVVGERVRRVE
                     TGQLQAYGASFIVGAAVVAAGILIVNP"
     gene            complement(3522..3716)
                     /locus_tag="VNL92_01555"
     CDS             complement(3522..3716)
                     /locus_tag="VNL92_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93428.1"
                     /translation="MWSKLFEVKNAYMAEAWQELLRSEMLSVQIVPDLGDPNASAFTP
                     RALYVPDSKTHVAAEVLRKI"
     gene            complement(3720..4034)
                     /gene="nuoK"
                     /locus_tag="VNL92_01560"
     CDS             complement(3720..4034)
                     /gene="nuoK"
                     /locus_tag="VNL92_01560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012306551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWO93429.1"
                     /translation="MEIELEHYLAVGALLFALGLLIALSKRNAIGALMGIELMLNAVN
                     LTFVSFARFAESSEPIAGQVFAVFVITIAAGEAALALALAVAVYRRRRSIDVEDIDLL
                     KW"
     gene            complement(4035..4532)
                     /locus_tag="VNL92_01565"
     CDS             complement(4035..4532)
                     /locus_tag="VNL92_01565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWO93430.1"
                     /translation="MDDVGIVIAFWALSVMTVGSALLVVLFRNLLHAVLSLIVSFAGM
                     AGLFVTLSADFLAVSQVLIYVGAISILMIFAIMLTPEGGRRNTESRYLPAAVVGALGI
                     VAAVMFVVFDVDWATPDREGFEETASLLGEALLDKWVLPFEIASAVLLAAMIGAIVLV
                     RERGD"
     gene            complement(4801..5458)
                     /locus_tag="VNL92_01570"
                     /pseudo
     CDS             complement(4801..5458)
                     /locus_tag="VNL92_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
BASE COUNT         1220 a         1778 c         1681 g          779 t
ORIGIN      
        1 cacgaacagg tccgccagga tgacgacgcc cgccgttgct aggacgatga tgacgggggc
       61 aagcaggtcg atatcgtcgg ccaagggcct tctcctctag ccgctacaac cggccggcga
      121 tcggctctat gcccatctcg ataacgtccg tcaggaccgc tgggtagacc ccgacgacga
      181 cgatggcgac cacgagcgcc gccatcggca cgcgctccca ccactggtcg gcatcggtga
      241 ggtgacccca ccgctcctcg tcgacgggtc catggaagac tcgctcaatc gtccacagga
      301 tgtacccagc agccagaacc acgccagtcg ccacggcgat cgtcgcggcc tcgtgccgtc
      361 cgaacgagcc caggaacacg gtgagctccg ccgcgaatcc ggacagggcg ggcagtccca
      421 gcgaggcaaa tcccgcgatg agggtgacga gcgccgctac gggcatgcgg gatgcgaggc
      481 cgcgcatctc cgggatttgg cgcgtgtgcg ttcggtcgta gatgacgccc accatgagga
      541 acagcagtcc ggtgatggtg ccatgggtga acatctgcag cgtcgcgccc gacaggctcg
      601 tgctcccgag cgcggccacg ccgagcaaca cgaagcccat atgactgacg gagctgtagg
      661 cgatgagtcg cttcaagtcg gtttgccgca gcgtcaccaa ggccccgtac actacgctcg
      721 tcgccgcaag cgcagcgagc agcacatccc aatcgcgggc aacgtcgggc atgagcccga
      781 tgcagaatcg gatgatgccg tagccaccca tcttcagcag gacacctgca agcatcacgg
      841 agacagccgt gggcgcatcg gtgtgggcat cgggcagcca cgtgtggacg gggaacaccg
      901 gcagcttgat ggcaaacgct gcgaagaagc cgaagaagat gagctccacc ggaatctctg
      961 tgtccgcgat cgtcatctgc gacagctcgg tgtagtcgaa cgtgccctgg gagaagccga
     1021 gggccaggat cgctgcgagc atgaagcccg agccgaacag cgtgtacgcg aggaacttga
     1081 tcgccgagta ttccttacgc ccggtccccc agatggagat gagcagatac atggggatga
     1141 gctccacttc ccagaagagg aagaagagca cgagatcgag cgacgtaaag acaccgagaa
     1201 cgcccgtctc cagcaccagg agccacacga agtactcccg agcgcgggtc tcgatacccc
     1261 acgagatgag aacagcgatg aacgtcagga ggccggtgag cagaagcagg gtgacgctga
     1321 tgccgtcgac gcccatgagg tacttgatct cgaagttgtc cgcgtcgatc cacgtgaact
     1381 cctgctcgaa ctggaagcca ccgtcgtccc gatcgaagag gacgaacatc acgaccgtcg
     1441 cgacgaacac cacgaacgtc gtgaccgcag cggtgacgcg cggacggatc ggagcgctcg
     1501 atggcgtcaa cgccacgacg agcgctccca gcaacgggaa gaagacgacg aacgatgtca
     1561 tgctacgggt tcacgatgag tattcccgcg gccaccaccg cggccccaac aatgaacgag
     1621 gctccatagg cctgcagctg ccccgtctcg acgcgtcgca cccgctcccc aacgacatcg
     1681 cctgtgcgtg cggcaccgtt cacgatgcca tcgacgacat actgatcgaa cagggccagc
     1741 gcccgcacaa gcccagcgta gaagagccag cgaaccacca ctgtctcgta gagcgcatcg
     1801 aggtagtagc gattcgaaag gagcgtccac gcgggacgca gccgcgcccc cagccactca
     1861 gcagacatga cccgcagcgg ctcgtagtag attgccgcag ccgtgaatac gcccaaaagc
     1921 gccacgccgg tggaggctgt ggcgatcggc cattcgaatt cgaactcgtg gacttccacc
     1981 ccttccggca aagcgccctc cacgaggtgc gtaaagtcgt ggtcgatgtt ccagaacccg
     2041 acgagcactg cggggacggc gaggacgacg aggggcagcg ccattaccca ggggctctcg
     2101 tgggggtggg cttcgtgcgc ctccgctgcg tgctcctcgg cgtcgccccc tgcatggcca
     2161 tcggcggccg ccggctcacc cccgcggtac tcgcccgcga aggtcatgaa gatggcccga
     2221 aacatgtaga aggcggtcag gaacgcggct ccgagaagca cgaggaagta cgcccaattc
     2281 tcgtcgaagg ctccggcgag cacctcgtcc ttgctccaga acccggcgaa cggcggtatg
     2341 cccgagaggc tgagcgctcc gatgacgaag gtggcggcag tcacgggcat agcccgggcc
     2401 aagcctccca tcctccgcat gtcgaacgta ttcgtcgcgt gattgacgga gccggacccc
     2461 aggaacagca acgccttgaa gaaggcatgc gtcatcaggt ggaatatcgc ggcaacatac
     2521 gcgccgacgc ccagagcgac catcatgtat ccgagctggc tgattgtgga gtaggccaga
     2581 actcgcttga tatcgaacat gacgacgccg agcagcgcag cgcctagtgc cgtgatcgac
     2641 ccgatgaccg cgaccgtcgt catcgcctca tcggacacgc tgaacaccgg gaacagccgc
     2701 gcgacgagat agacgccggc ggcaaccatc gtcgcggcgt ggatcaaggc cgaaacgggc
     2761 gttgggccct ccatcgcatc cggcaaccac acgtgcagcg ggaactgcgc gctcttgccg
     2821 gccgcgcccg cgaagacgcc gaccgcgaac cacgagagca tggccgatcc gatggcgccg
     2881 gccagcgctg cctcgtggat ggcctcgatg tcgaacgtgc cgatctcgcg atagatgagc
     2941 aggatggcga ggaggaagcc gaggtcgcca agcctggtga cgataaatgc cttcttcgcc
     3001 gccgccgccg cgcttggccg ccggaaccag aagccgatga gcagatacga cgaggcgccc
     3061 accagctccc agaacacgaa tagcatgatc aggttgtcgg cgaggacgag gccaagcatg
     3121 gccgccgtga acagcgacat cacggcgaag tagcgcttgt accccgggtc gccatgcatg
     3181 tagccgacgg aatagacctg caccaggagc gagacggccg acacgaccac gagcatgacg
     3241 gacgtcaggc catcgaggcg gatgccgatg ttgacggtca ggtcgccgag cgaaagccat
     3301 tcgtgcgtgc cgaagccaat gatgtgccca tcggcgtcga tgccgctatc gagcgcccaa
     3361 agagcgagac caaaggagcc caccaaggcg gcgatgatgg tcagcgcggc gaactcgtcg
     3421 ggcgtccgga gcgcccactg aacagcgatg agggcgagag aggcgacagg aagaaagaag
     3481 atcgcccaca ccgctgcctc gttgatcgta tcgaacatcc gctagatctt ccgcagaacc
     3541 tcagctgcca cgtgggtctt actgtcgggc acatagagcg cgcgcggggt gaatgcactg
     3601 gcgttcggat cgccaaggtc cggtacaatc tgcaccgaga gcatttcgct tctcagcagc
     3661 tcttgccacg cttctgccat gtaggcgttc ttcacctcaa acaacttcga ccacatacgt
     3721 caccacttca acaagtcgat gtcttccaca tcgatgcttc ggcgccgccg gtagacagcg
     3781 accgcaagcg ccagagcgag cgccgcttcg cccgcagcga tcgtgatcac gaacacggcg
     3841 aagacctggc cagcaatggg ctccgacgac tcggcgaacc gcgcgaagga aacgaacgtg
     3901 agattgaccg cgttcagcat caactcgatg cccatgaggg cgcctatcgc gtttcgcttc
     3961 gagagcgcga tgagcaggcc cagggcaaac agaagcgccc cgacagcaag gtagtgctcg
     4021 agctcgatct ccacctaatc gcctctctca cgcacgagca cgatcgcacc aatcatggcg
     4081 gccagcagaa ccgcactcgc gatctcgaag ggcaacaccc atttgtccag caacgcctcg
     4141 ccaagcaggg acgctgtctc ctcaaagccc tcgcggtcag gcgtcgccca atcgacgtcg
     4201 aagacgacga acatgacagc agctacgatg cccagggcgc cgaccacggc ggcgggcaga
     4261 taccggctct cggtattcct ccggcctcct tccggcgtca gcatgatggc gaaaatcatg
     4321 agaatcgaga tggccccgac gtagatcagc acctgcgaca cggcgaggaa atcggccgaa
     4381 agcgtgacga acaggccggc catgcccgca aaagacacga tgagcgacag caccgcatgg
     4441 agcaggttcc gaaacagaac gaccagcagc gcggaaccca cggtcatcac ggacaacgcc
     4501 cagaaggcga tgacgatgcc tacgtcgtcc atccttggtc ctcgggtacc gtcccaagca
     4561 ggtgggacac tgatcgaagc gcctgcttcg gacccgtcac atgccggata gtccgccagg
     4621 aaagccctcg gtggcgggac cggcaacgaa gccgatgtcc cgcgccagcg tcacgctgtc
     4681 gtccggctta tcgtatccat acgcgcgggc atacgcgacg acgagcgcca cagccgcgat
     4741 ggcgttgatg acgccgaaga tgggcagcgc cacctccgtc ggccaatcgt tctcctgcca
     4801 cagcaagact tcgagcgcga cggcgaacac gttgaccagc gaaagcggta ggaggaactt
     4861 ccacgcgaac gccagcagct ggtcgatgcg caggcgcggg agagtgcccc gcaaccagac
     4921 gagcaccatg aagaagacgt agatcttgcc gatgaatagg agccagcccg gaacccactg
     4981 ctccaacccc caggtccacc agccaccgaa ataaaccgtg gccacgaggg ccgagatggc
     5041 cagcgcgttc gtgtactccc ccgcatagaa cagcgagaac ttcatgcccg agtattcggt
     5101 gtggtagccg gccacgatct ccgattccgc ctccgcgatg tcggccgggg tccggttcaa
     5161 ctccgccgtt gcggagatgt agtacacgag gaacgccatc ggctgcagca ggatcagcca
     5221 cacgttgttg tcctgctgcc attcgacaat cccctggagc gagaggctcc cggtaaagag
     5281 cgacacgccg agcagcgcaa cgacgagcgg cacctcgtac gagatcatca tggcgataac
     5341 ccgcatcgct ccgagcaacg agtacttgtt gttcgacgac cacccggcca taaagaccgt
     5401 gaccgtcgtg accgagctca gggcaacgaa gtagatgagg ccgacgttca ggtccgcg
//
LOCUS       DATMBB010000074         2031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_545320, whole genome shotgun sequence.
ACCESSION   DATMBB010000074 DATMBB010000000
VERSION     DATMBB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545320"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VNL92_01575"
     CDS             <1..733
                     /locus_tag="VNL92_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93431.1"
                     /translation="AFVRGKVHGFEMDEAEVPAEQRARAELTARRCFELALSYASTRR
                     DRPLVMAMCGEVGTGKSHIAAALAGRVGAALLRSDVVRKEVAGLAPTARLRSGFDEGL
                     YSPQHQARTFNALFARAHRYLGDGHNVVLDATFARRDQRRRARNLAHRSGADYVVIEC
                     VAPPEVIIQRLHQRAAESVDASDATVEVYERLHGRYQPVRDDEGPAIRVDTTLPLGET
                     LPLILAALAESPPRNASPPSPWWHS"
     gene            795..>2031
                     /locus_tag="VNL92_01580"
     CDS             795..>2031
                     /locus_tag="VNL92_01580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93432.1"
                     /translation="MLQSVDVGERSIEAYRGIVPDNTLDELRAQAASLRGARVLHINA
                     TPYGGGVSELLRSVVPLQSDLGIIADWRIVSGDRDFFEATKAMHNALQGDPQGLSEGQ
                     RQAYVANAQRNADQMQEEYDFVIVHDPQPAALLHLRGHDSARWIWRCHIDTSEPNVDV
                     WSFLRPFLADYDAAVFTLPEFIPPDLPVDLVEVIPPAIDPLSPKNLPLPESLARQILG
                     WIGIELGRPLITQVARFDPWKDPLGVIDAYRLARQSVPNLQLALVGSMALDDPEGWTI
                     YERILEESRSDPMIHVFSNLTGVGNIEVNAFQRLSDVVVQKSLREGFGLVVSEAMWKG
                     TPVVAGRAGGIPLQMADGVGGILVDGVQECADAIVRLLEHPDEGTSLGERGRERVRQH
                     FLLPRLLLNEVSLMNDLLRV"
BASE COUNT          327 a          694 c          667 g          343 t
ORIGIN      
        1 agccttcgtg cggggcaagg tgcatgggtt cgagatggac gaggccgagg ttcccgccga
       61 gcagcgtgct agggccgagt tgacggctcg ccgctgcttc gagctcgcct tgtcgtatgc
      121 gagcacgcga cgcgaccggc ctctggtcat ggcgatgtgc ggcgaggtgg ggacggggaa
      181 gagccacatc gccgcggcgc tcgccggacg tgtgggcgcc gcgttgctac gctccgatgt
      241 ggttcggaag gaggtggcgg gtctcgcgcc gacggcgcgc ttgcgctccg gcttcgacga
      301 agggctctat tctccccagc atcaagcccg cacgttcaac gcgctcttcg cacgagctca
      361 ccgatacctg ggcgatggcc acaatgtcgt gctcgacgcg accttcgccc gccgcgacca
      421 acgacgcagg gcacgcaacc tcgcacaccg aagcggagcc gactatgtcg tgatcgagtg
      481 cgtcgcgcca ccggaggtca tcatccaacg gcttcaccag cgagcggcgg agagcgtcga
      541 cgcctcggac gccaccgtcg aggtatacga gcggctccat ggccgctacc agcccgtgcg
      601 ggacgatgaa gggccggcca tacgcgtgga taccacgctc cctctcggcg aaaccctgcc
      661 cctgattctc gcggcgctgg cagagagccc tccccgcaat gcatcgccgc cctcgccttg
      721 gtggcactca taacgattga tgcggcccca gggccgttcc cgcatcgaac gcccggcgtg
      781 taagctagag gcgcatgctg cagagcgtcg acgtcggcga gcgctccatc gaggcgtacc
      841 gtgggatcgt tcccgataat acgctcgacg agctgcgtgc ccaggcggct agcctgcggg
      901 gggcacgcgt cctgcatatc aacgcgactc cctacggggg cggcgtatcc gagctcctcc
      961 ggtccgtcgt gccgctgcaa tcggatctgg gcatcattgc cgactggcgg atcgtatccg
     1021 gcgatcgcga cttcttcgag gccaccaagg ccatgcacaa cgcgctgcag ggcgatcctc
     1081 aaggcctcag cgagggtcag cggcaggcct acgtggccaa cgcgcagcgg aacgccgatc
     1141 agatgcagga ggagtacgac ttcgtcatcg tccacgaccc gcagccggcg gcgctgctcc
     1201 acctgagggg ccacgattcg gcccgctgga tctggcgttg ccacatcgat acgtcggagc
     1261 ccaacgtcga cgtctggagc ttcctgcgtc ccttcctcgc cgactatgat gccgccgtct
     1321 tcaccttgcc ggagttcatc ccgcccgacc ttcccgtcga cctcgtcgag gtgatcccgc
     1381 cggccatcga ccccctcagc cccaagaacc tgccattgcc cgaaagcctg gcgcggcaga
     1441 ttctcggttg gatcgggatc gagctggggc ggccgctcat cacgcaggtc gctcggttcg
     1501 atccctggaa ggacccgctg ggcgtgatcg acgcgtaccg cctcgcgagg cagagcgtcc
     1561 ccaacctcca gctcgccctt gtcggatcga tggcgctcga cgacccggag ggctggacca
     1621 tctacgagcg catcctcgaa gagtcccgct ccgatccgat gattcatgtc ttctcgaacc
     1681 tcacgggagt cgggaacatc gaggtcaacg ccttccagcg gctgtcggac gtcgtggttc
     1741 agaagtcgct ccgggaggga ttcggcctcg tcgtatctga ggcgatgtgg aagggaacgc
     1801 ccgtcgtcgc cggccgggcc ggcggcatcc ccctgcagat ggccgacggc gtcggcggca
     1861 tcctggtgga cggcgtgcag gagtgcgcgg acgcaattgt gcgcttgctg gagcacccgg
     1921 atgaaggcac gtctctgggg gagcgcgggc gcgaacgggt ccgccagcac ttcttgctac
     1981 cgcgcctcct gctcaacgaa gtgtcgctca tgaacgactt gctgcgcgtt c
//
LOCUS       DATMBB010000075         4306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_345100, whole genome shotgun sequence.
ACCESSION   DATMBB010000075 DATMBB010000000
VERSION     DATMBB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345100"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VNL92_01585"
     CDS             complement(<1..737)
                     /locus_tag="VNL92_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO93433.1"
                     /translation="MNVISFERTGLGNSSYLVDIGGGRAVAVDPDRTAQRYITAAEER
                     GLRIVAALETHVHADFVSGARELAAAGADILAPAESELRFPYRGLHASERFILDGVQV
                     EAIASPGHTPEHLSYVLRADGRAPALFSGGSLIVGGAARTDLITPEMTEPLTRAQFRT
                     LHKAFGHLPDETELYPTHGGGSFCSVGGTSSSTSTLGEERRSNPLLAMDDEEEFVRSW
                     PATFPAAPAYFFRMRAFNQAGPRLRRE"
     gene            complement(734..2014)
                     /locus_tag="VNL92_01590"
     CDS             complement(734..2014)
                     /locus_tag="VNL92_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93434.1"
                     /translation="MNSIELRGSARDTGRTITLGLRANWRQFALLVAVNAFVGGMVGL
                     ERSIVPLLAEEEFGVASKAAAVSFIATFGLAKAIANLYAGRLSERFTRKSVLMAGWLF
                     GLPVPFILIAAPSWEWVVAANALLGLNQGLAWSMTVNMKMDLVGPERRGLALGLNEAA
                     GYLAVGLAAFATGAIADRAGLRPEPFYLGIGFAAIGTALSALAVRDTSAHVGIESALH
                     PTRGTAPSLPRAFADATWRRPQLFGISQAGFANNLNDALAWGIFPLFFAANGMRLGEI
                     GLLAAVYPFVWGALQVVAGWASDRTGRRPLIVAGMLAQAAAIVVVAASRGSFAAALIG
                     VAALGIGTALVYPTLLAAVGDLAHPNERATLLGVYRFWRDAGAMAGAVAAGALADILG
                     YEAAIAAVAALTALSGLVAAATLQGRPSVQEGLT"
     gene            complement(2127..2486)
                     /locus_tag="VNL92_01595"
     CDS             complement(2127..2486)
                     /locus_tag="VNL92_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWO93435.1"
                     /translation="MAKILVNCTHGKEDPERAILPFVVGNVSATADQETVVLLTIDGV
                     WLATKGYAEGVQQEGFQPLTEVMASFLANGGQLWACGACTNPRGITERDLIEGAKIVT
                     AANVVEYMASGASTLTI"
     gene            complement(2533..2784)
                     /locus_tag="VNL92_01600"
     CDS             complement(2533..2784)
                     /locus_tag="VNL92_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWO93436.1"
                     /translation="MVDEENVPSPDALLDAPGEACATLTPLIREKMRELQSEAVLEVR
                     SDDPAAREGVPAWSRLTGNSLLATIEEDERTTRFYLRKK"
     gene            complement(2963..3574)
                     /locus_tag="VNL92_01605"
     CDS             complement(2963..3574)
                     /locus_tag="VNL92_01605"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO93437.1"
                     /translation="MTNVVAAKVDRDGLRQQIQSKYTEVAVEPEKGFHFHTGRQLAAM
                     LGYDLTEVDRLPVGTVNSFAGTGNPFLMGRLQPGETVLDIGCGAGFDTLQAARQLGPD
                     GRVIAVDMTEAMLSRTSEGAAELGLTNVIVRHGFAENLPAPDGSVDVVISNGVINLCP
                     DKVGVMREVWRVLRPGGRFQIGDIVVHEAVPQSAKDDIELWSG"
     gene            3775..>4306
                     /locus_tag="VNL92_01610"
     CDS             3775..>4306
                     /locus_tag="VNL92_01610"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWO93438.1"
                     /translation="MNHREFKDKLYAQFARVGQALASERRLELIDLLAQAPRHVDALA
                     EETGQSVANVSQHLQTLRHAHLVETEREGTKIIYRLAGDDVLRLWLALRSVAEARLPE
                     VGVLADEFAIDGGGGEVGREELDRLLQRDDVVVIDVRPAHEFRHGHLPTAISIPVEEL
                     PERIAELPRDRRIVAYC"
BASE COUNT          743 a         1462 c         1395 g          706 t
ORIGIN      
        1 atctcgcggc gcaatcgagg acccgcctgg ttgaaggcac gcatgcggaa gaagtatgcg
       61 ggggccgcgg gaaacgttgc cggccacgac cgcacgaact cttcctcgtc gtccatcgct
      121 aggagtgggt tggaccgtcg ctcctcgccc agcgtcgatg tgctgctgct ggtgccgccg
      181 acggagcaga acgagccgcc gccgtgcgtg gggtagagtt cggtctcgtc cgggaggtgg
      241 ccgaacgcct tgtgcagggt gcggaactgg gcgcgggtca gcggctccgt catctccggc
      301 gtgatgagat ccgtgcgggc ggcccctccc acaatgagcg aaccgccgct gaacagcgct
      361 ggcgcacggc cgtcggccct caggacgtag ctcaggtgct cgggcgtgtg ccccggcgag
      421 gcaatagctt cgacctgaac gccgtccagg atgaaacgct cgctcgcgtg caggcctcgg
      481 tacgggaacc gaagttccga ctcagccgga gcgaggatat cggcgccggc cgcggccagc
      541 tcacgggcgc ccgagacgaa gtcggcgtgg acgtgcgttt caagcgcagc gacgatgcgc
      601 aggccgcgct cctcggcagc cgtgatgtaa cgctgggctg tgcggtcggg gtcgacggcg
      661 acggcgcggc caccgccgat atcgacaagg tacgagctgt tgcccagtcc ggtgcgttcg
      721 aacgatatca cgttcatgtc agaccctcct gaactgaagg ccggccttgg agcgtcgccg
      781 cggccacgag cccggatagc gctgtgagcg ccgcgacggc cgctatggcg gcctcgtatc
      841 cgaggatgtc ggccagggcg ccggccgcaa ccgccccagc catcgcgccc gcgtcgcgcc
      901 agaatcggta cactcccagc agcgtggctc gctcgtttgg gtgagccagg tctccgacgg
      961 cagcgagcaa cgtaggatag accagcgccg tcccgatgcc gagcgctgcg acgcctatca
     1021 gcgccgcggc gaaggaaccg cgggaagcgg cgacgacaac aatcgcggcc gcctgcgcca
     1081 gcataccggc aacgatgagc ggacggcgtc ccgtccggtc gctggcccag ccagccacca
     1141 cctgcagcgc tccccatacg aaaggataca cggccgccag caggccgatc tcgccgagcc
     1201 tcatgccgtt cgcggcgaag aacaaaggaa agatgcccca agcgagggcg tcgttcaggt
     1261 tattcgcgaa accggcctga ctgatgccga atagctgcgg ccggcgccac gtcgcgtcgg
     1321 cgaacgcgcg cggcagcgat ggcgctgtgc ctcgcgtggg atgcagcgcg gactcgatgc
     1381 caacgtgggc cgaggtatcg cgcacggcca acgccgagag ggccgtgcct atggccgcga
     1441 acccgatgcc gagatagaac ggctcgggcc gcagaccagc ccggtcggcg atggcgcctg
     1501 tggcaaaggc ggcaagcccg acggcaagat acccggcagc ctcgttcaac ccaagcgcga
     1561 gtcctcgccg ctccggtccc accaggtcca tcttcatgtt gaccgtcatg gaccaggcca
     1621 gcccctgatt caaaccgagc agggcgtttg ccgcgaccac ccactcccag gatggcgcgg
     1681 cgatgaggat gaacggcacg gggagcccga agagccagcc ggccatgagg accgacttgc
     1741 gcgtgaaccg ctccgagagg cgcccagcgt acaggttggc gatcgccttc gcgagcccga
     1801 acgtcgcgat gaacgagacc gcagccgcct tcgacgcaac accgaactcc tcctcagcaa
     1861 gcaggggcac gatcgaccgc tccaggccga ccatgccccc aacgaaggcg ttaacggcga
     1921 ccagcagggc gaattggcgc cagttcgccc tgaggccgag tgtgatggtg cgccccgtgt
     1981 cccgcgcgga cccgcgcagc tcgatagagt tcatctagcc tgccggcggg gcgaccggct
     2041 cacgtctcct tccctgttca gactgcgtgc cgctggttcc tcatccgtcg ctcgcacgct
     2101 gcgaggcgtt atcgctcgtc cgcggatcag atggtcagcg tcgatgcgcc gcttgccatg
     2161 tactccacca cgttcgcggc cgtgacgatc ttggcgccct cgatgagatc gcgttccgtg
     2221 atgccgcgcg ggttcgtgca tgcgccgcat gcccagagtt ggcctccgtt cgccaggaac
     2281 gatgccatca cttcggtgag cggctggaac ccctcctgct gaacgccctc tgcgtatccc
     2341 ttcgtggcga gccacacgcc gtcgatggtc agtagcacga cggtctcttg gtcggcggtg
     2401 gccgacacat tgcccacgac gaacggcagg atggcccgct ccgggtcctc cttgccgtgc
     2461 gtgcagttca cgaggatctt tgccacggtg tgcctcctta agctccaccc ggccccggaa
     2521 gaccggctga cgttacttct ttctcaggta gaacctcgtt gttcgttcgt cctcctcgat
     2581 tgtggcaagc agggaattgc cggtgagccg gctccaggcc gggacgccct cccgcgctgc
     2641 cggatcgtcg gagcgaacct ccagaacagc ttcgctttgc agctcacgca tcttctcccg
     2701 aatcagcgga gtgagcgtgg cgcaagcttc gcccggcgca tccaggagcg catcgggcga
     2761 aggcacgttc tcctcatcga ccatcagcat cctccccggg ctttcgcccc gcaaacgaga
     2821 cgccccgcgt cccgaacttg ctggcatccg actcatgcct cgagccggag aacacatcga
     2881 cttccgggcc ccaacgaaca tcgacgaagc cgacatgccc caacacccat cgccactctg
     2941 cttccagcag agcacctgct attcagcctg accacagctc gatgtcgtcc ttcgcagact
     3001 gggggacagc ctcgtggaca acgatgtcgc ctatctgaaa ccgtccgccg ggccggagca
     3061 cgcgccacac ctcgcgcatc acgcccacct tatcggggca gaggttgatg acgccgttgc
     3121 tgatgaccac gtcgaccgag ccatccggcg cgggcaggtt ctcggcgaac ccatggcgga
     3181 cgatgacgtt cgtgagtccg agctctgcgg cgccctcgct cgtgcgcgag agcatcgctt
     3241 cggtcatgtc gacggcgatg accctcccat cggggccgag ctgacgagcc gcctggagcg
     3301 tatcgaaccc cgccccacac ccaatgtcga gtacggtctc gccgggctga agccgaccca
     3361 tgaggaaggg attcccggtg ccggcgaagg agttgacggt tcccaccggg agtcgatcca
     3421 cctcggtgag gtcatagccc aacatggcag cgagctggcg tccggtatgg aagtggaatc
     3481 ccttctccgg ctcgacggcg acttcggtgt acttggactg aatctgttgg cgcaggccgt
     3541 ccctatcgac cttggccgcg accacgttgg tcatgactgc ttgctcctga caaatcggct
     3601 acctgccgga cgcgctgtcg atcggggtcg taacgcgttc gatttgcttg tcaggccctc
     3661 ctttcgtccg ctatcgctgt gccacgacgc gaggagtgtg ccatagaatc ttcggcttgt
     3721 caagcgataa atggaacagt tgcagaacgt gcacgccact gctatcctgc gctggtgaac
     3781 catcgggagt tcaaggacaa gctgtacgcg cagttcgccc gcgtaggcca agccctggcg
     3841 agcgagcgac gcctcgagct tatcgatctc ctcgcgcagg cccctcgcca cgtggacgca
     3901 ctggcggagg agacgggtca atcagtggcc aacgtgtcgc agcacctgca gacgctgcgg
     3961 catgcgcacc tcgtcgagac cgaacgtgag ggaacgaaga tcatctatcg gctcgccgga
     4021 gatgacgtcc ttcgcttgtg gcttgcgctg cggtccgtcg cggaggcacg cctgcccgag
     4081 gtgggcgtgc tcgcggacga gttcgcgatc gacggtgggg gaggagaggt cggccgcgag
     4141 gagctggacc ggctgctgca gcgggatgac gtcgtggtga tcgatgttcg gcccgcgcac
     4201 gaattccgtc acgggcatct cccaacggcg atctcgattc cggtcgagga gctgccggag
     4261 cgcattgccg agctgccccg cgaccgccgc atcgtcgcct actgtc
//
LOCUS       DATMBB010000076         2493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_495484, whole genome shotgun sequence.
ACCESSION   DATMBB010000076 DATMBB010000000
VERSION     DATMBB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495484"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /gene="glmS"
                     /locus_tag="VNL92_01615"
     CDS             <1..1567
                     /gene="glmS"
                     /locus_tag="VNL92_01615"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWO93439.1"
                     /translation="RIVVVHNGIIENYLELKRELQQHGHQFKTETDTEIVAHLVEREM
                     RDDGLEEAVRRALRVMRGMFAIVLVSVEDPEKIVAVRNGPPIVVGLGDNEFFVASDIP
                     AILSHTRDVVFLGDEEMAVITRSGVTFTDYSGRAVSKTSQRVLWDPIAAEKGGHRHFM
                     LKEIFEQPTAARDTILGRVSIDRGQVFLEDLNIPNDAFRAFEKVTIIACGTSWHAALV
                     GKYLIEALAKIPVEVDYGSEYRYRNPIISSKELTVVITQSGETADTLAALREAKRKGA
                     RSIAICNVVGSMVTREADGTIYTHAGPEIGVASTKAFTSQLVALQLLALHMAQVRNTV
                     PADDIRCHIEDLLRIPQLIEHAIRASAPIEKVAERFHNRTDFLFLGRGINYPIALEGA
                     LKLKEISYIHAEGYPAGEMKHGPIALIDDRMPVVAIAPDDQVFEKMIGNVQEAKARGA
                     SVIAITTDGDDRMASVLDAANHVIVPMPRTTPLLTPIIMTIPLQLLAYYIAVWRGCDV
                     DQPRNLAKSVTVE"
     gene            1574..>2493
                     /locus_tag="VNL92_01620"
     CDS             1574..>2493
                     /locus_tag="VNL92_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWO93440.1"
                     /translation="MDLLSSLNPEQQAAVLKTDGPLLILAGAGSGKTRVIAHRIAYLV
                     SAGLAEPDRILAVTFTNKAAEEMRARVEALLGMDCRQMWVSTFHALCARLLRREAPHI
                     GLSRDFVIYDSTDQLTVVKQALKSLGIDDGVTQPRAALSRISHAKNRMEGAAAFSNAA
                     WNPREQQIARIYELYTKALADANALDFDDLLLRTVELFQRSEQVRERYSRKFRYLMVD
                     EYQDTNRPQYLLIQQLAAAHRNLCVVGDPDQSIYKWRGADLRNILDFEHDFPEATTVK
                     LERNYRSTQVILDAATAVISKNRKRKDKRL"
BASE COUNT          476 a          825 c          783 g          409 t
ORIGIN      
        1 tcgcatcgtc gtggtgcaca acggcatcat cgagaactac ctcgagctga agcgcgagct
       61 gcagcagcac gggcaccagt tcaagaccga aaccgacacc gagatcgtcg ctcacctggt
      121 cgagcgcgag atgcgcgacg acggcctcga ggaagcggta cgccgcgcgc tgcgggtcat
      181 gcgcggcatg ttcgcgatcg tgctcgtctc ggttgaagat ccggagaaga tcgttgccgt
      241 ccgcaacggg ccgccgattg tcgtcggcct cggcgacaac gagttcttcg tcgcgtcgga
      301 cattcccgcg atcctgagcc acacgcgcga cgtcgtgttc ctgggcgacg aggaaatggc
      361 tgtcatcacc cgttcgggtg tgactttcac cgactactcc gggcgtgcag tctccaagac
      421 ctcgcagcgt gtgctctggg atccgatcgc ggcggagaag ggcggtcatc gccacttcat
      481 gctcaaggaa atcttcgagc agccgacggc cgcgcgcgac acgatcctcg gccgagtctc
      541 gatcgatcgc ggacaggtct tcctggagga cctcaacatc ccgaacgacg cgttccgggc
      601 gttcgagaag gtcaccatca tcgcgtgcgg cacgtcgtgg cacgccgcgc tcgtgggcaa
      661 gtacctgatc gaggcgctcg ccaagattcc ggtcgaggtc gactacggat ccgagtatcg
      721 ctaccgcaac ccgatcatca gctcgaagga gctcaccgtc gtgatcacgc agtcaggtga
      781 gacggccgac acgctggcgg cgctccgcga ggccaagcgc aagggcgctc gcagcatcgc
      841 gatttgcaac gtggtcggga gcatggtgac gcgcgaggcg gacggcacga tctacacgca
      901 cgcggggcct gagatcggcg tggcctcgac caaggcgttc acgtcgcagc tcgtcgccct
      961 ccagctgctc gcgctgcaca tggcccaggt gcggaacact gttcccgccg atgacatccg
     1021 gtgccatatc gaggacctgc tgcgcatacc gcagctcatc gagcacgcca tcagagcctc
     1081 ggcgccgatc gagaaggtgg ccgagcggtt ccacaaccgg accgacttcc tgttcctcgg
     1141 ccgcggcatc aattatccga ttgcgctgga aggagcgctg aagctcaagg aaatctccta
     1201 catccacgcc gagggctacc cggccggtga gatgaagcac ggaccgatcg ccctcatcga
     1261 cgatcgcatg ccggtcgtcg cgattgcgcc cgacgatcag gtattcgaga agatgatcgg
     1321 caacgttcag gaggccaagg cgcgcggcgc ctcggtcatc gcgatcacca ccgatggcga
     1381 cgatcgcatg gccagtgtgc tggacgcggc caatcacgtg atcgtgccga tgccgcgcac
     1441 gacgccgttg ctgacgccga tcatcatgac cattccactt cagctgctgg cctactacat
     1501 cgccgtctgg cgcggctgcg acgtcgatca gccgaggaat ctcgccaaga gcgtaacggt
     1561 agaataggag gtggtggatc tcctctcctc tctcaatccg gaacagcagg cggccgtcct
     1621 caagaccgac ggcccgctgc tcatcctcgc cggggcgggt tcgggcaaga cgcgcgtcat
     1681 cgcgcaccgc atcgcgtatc tcgtcagcgc gggactcgcc gagccggacc ggatcctcgc
     1741 cgtcaccttc acgaacaagg cggccgaaga gatgcgggcc cgcgtcgagg ccctgctcgg
     1801 catggactgc cgccagatgt gggtgtcgac gtttcacgcg ctctgtgcgc ggctgctgcg
     1861 ccgggaggcg ccccacatcg ggctgtcccg cgatttcgtc atctacgact ccactgacca
     1921 gctgacggtc gtcaagcagg cgctgaaatc gctcggcatc gacgatggcg tcacgcagcc
     1981 acgcgcggcg ctgtcccgga tcagccacgc gaagaatcgc atggagggcg cggccgcgtt
     2041 cagcaacgcc gcctggaatc cgcgcgagca gcagatagcc cggatctacg agctctacac
     2101 gaaggcgctt gcggacgcga acgccctgga cttcgacgac ctgctgctca ggacggtcga
     2161 gctgttccag cgctccgagc aggtgcgcga acgctacagc cgcaagttcc gctatctgat
     2221 ggtcgacgag tatcaggaca cgaaccggcc tcagtacctg ctcatacagc agctggcggc
     2281 agcacaccgc aatctgtgcg tggttggcga cccggaccag tcgatctaca agtggcgcgg
     2341 cgcggatctc cgcaacatcc tcgacttcga gcacgacttt ccggaggcca cgacggtcaa
     2401 gctcgaacgc aactaccggt cgacgcaggt gatcctcgat gcggccaccg ccgtgatctc
     2461 gaagaaccgg aaaaggaagg acaagcgtct cta
//
LOCUS       DATMBB010000077         4360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_152475, whole genome shotgun sequence.
ACCESSION   DATMBB010000077 DATMBB010000000
VERSION     DATMBB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152475"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VNL92_01625"
     CDS             complement(<1..409)
                     /locus_tag="VNL92_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWO93441.1"
                     /translation="MDVLLASRIPDFGRPTYAGKVRDTYDLGDTLLIVATDRISVFDV
                     VLPTGIPGKGKILTQLSAWWFERTSAVVPNHFLRLVDSAHVEGVPFEVPRELWGRSML
                     VRKADRVDVECVVRGYLAGSAWEEYQARGTVQGF"
     gene            complement(580..1347)
                     /locus_tag="VNL92_01630"
     CDS             complement(580..1347)
                     /locus_tag="VNL92_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93442.1"
                     /translation="MPTLDGAQSWLDRAEEHLNELRALETMRLDYERERTTSGIKTHV
                     ETGSIAHYLAEGPPRTEDVDRARVLVGETIQALRRTLDYIVFQVAFLDSGSEQNDTQF
                     PIDEHPETFWRRLKRSERGTRDHSCYLIGVDRKHAAVFERYQPYKGTQWTPLLQAISN
                     RDKHRRLAIIIPSARVERGEVGQADESVQAGDDGFTYTLITKDGVYMKFPIRAFVTFE
                     DGLPVVQTLEELKAEVASVLEEFKPCMEGQCSHSAPP"
     gene            complement(1765..3099)
                     /locus_tag="VNL92_01635"
     CDS             complement(1765..3099)
                     /locus_tag="VNL92_01635"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93443.1"
                     /translation="MAYKLPLEGIRVVDVTVVWAGPYVTQLLAEWGAEVIRVEPLFHT
                     QPSTRGGEIPVTKEMVRAMKGSSNSMLTAFPNDDPAPRPWNRGPSFNSHARDKLSMTC
                     DVLVPGGLDIFRRLIAISDVYIENNVPETIEKAHITHEELVPYNEKLISLRMPSYGLS
                     GPYKNWRSFGTHMEGMTGHHYLRSYRHLDPSMVSDAFTVDAATGVMGAVAVMMALRHR
                     ARTGRGQLIEMAQAENFLPYLGEIILDYTMNGRVWEPQANRHPNHAPHNVYPCAGDDR
                     WIAIDCATDEEWAALCRVAGNPSWAHDERFKTSLGRLKHQDELDEAIGEWTRDKNDFE
                     LFHQLQAAGVTSGPVQNEADAFACPQLNERGFFEELTHPECGTHRYPGLMHKMSKTPN
                     KLKRHPVMLGEDNEYVYKKLLGFSDEEYRKLEEQGQIGLDPAGREPLPVTAD"
     gene            complement(3146..>4360)
                     /locus_tag="VNL92_01640"
     CDS             complement(3146..>4360)
                     /locus_tag="VNL92_01640"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93444.1"
                     /translation="TPLEGVRVLDLTHHIAGPHCTKILADWGADVLKIERPPGGDPSR
                     QYGPFHKDDPHPEKSALFLHLNTNKRSMTLNLKSAEGRSIVERLLPEFDIVVENFRPG
                     VMDSLGLGYQTLAAQNPRIILTSISNFGQTGPYRDLKASEITEFAMGGPMNVNGHREH
                     FPLKLGGNVVQYQAGGVAAYATLLALWQVEMHGAEGQHNDISIYETQAGFRDRRTTYL
                     VAHAYGGEIGRRPETAVRPGLGIRPCKDGYVNIYVTSGEKMKKFLSMIGREDLLSDPR
                     TDAGMMLGAMYPDLGEEIEASYLGWLMERGKLEAATIAQEHTLLAAPVNTVADLLNDP
                     SYRERGFFETIDHPATGPLTYPGRPFIMNDSPRPLARRAPLLGEHTKEVLCGRLGYSD
                     EDLTKLRAQGVI"
BASE COUNT          748 a         1383 c         1368 g          861 t
ORIGIN      
        1 ggaagccctg cacagtgccc cgagcctggt actcctccca tgccgagccg gcgaggtagc
       61 ctcgcaccac gcattcgaca tcgacgcgat ccgccttccg gacaagcatg gaccggcccc
      121 acagctcacg cggaacctcg aacggcacgc cttcgacatg agcggagtcg acgaggcgaa
      181 ggaagtggtt cggcacgacg gcgctcgtgc gctcgaacca ccacgcggag agctgggtga
      241 ggatcttccc cttccctgga atgcccgtag gcagaaccac atcgaacacc gagatgcggt
      301 ccgtcgccac gatgagcagg gtatcgccga ggtcgtaggt gtcgcgcacc ttgccggcgt
      361 acgttggccg cccgaaatcg gggatgcgtg atgcgaggag gacgtccaaa cgtggctccg
      421 aggcgcgacc gaactgtgcg catcgtagca cgccgacccg cgggtgtcag tcggcttcgc
      481 cgcggcctac gagccgggag atcagaggcg gaagaaggta ggaatgaatc cagcaactat
      541 cccattcctg tgtaatacag gcgatgcgaa cgcagcggtt cagggcggtg cggaatgact
      601 gcactgccct tccatgcacg gcttgaactc ctcgagcacg ctggcaactt ccgccttcaa
      661 ctcctcaagc gtctgtacga ctggcagacc atcctcaaag gttacgaagg cgcggatcgg
      721 gaacttcatg tacacgccat ccttggtgat gagggtgtag gtaaatccat cgtctccagc
      781 ctgaacactt tcgtcggctt gacctacttc tccgcgctca actctggcgc tggggatgat
      841 gatggccaag cgacggtgct tgtcacggtt cgagatggct tgaagaagtg gcgtccactg
      901 cgttcccttg tacggctgat accgctcgaa gactgccgcg tgttttcggt cgaccccgat
      961 gagataacac gagtggtcgc gagtcccgcg ctcgctccgc ttcagccgtc gccagaacgt
     1021 ctccgggtgc tcatcaatcg ggaactgcgt gtcgttctgc tcgctgccgg agtcgaggaa
     1081 cgctacctga aacacgatgt agtccagggt gcgccgcagc gcctggatgg tctctccaac
     1141 gaggacacga gcccgatcaa cgtcctcagt acggggaggc ccttcggcta ggtagtgcgc
     1201 gatgctcccc gtctcgacgt gagtcttaat cccgctggtc gttcgctctc gttcgtagtc
     1261 gagcctcatg gtttcgaggg cgcgcagttc attcaggtgt tcttctgccc ggtcaagcca
     1321 actctgagcg ccatcaagcg taggcatggg gtacactcga ccgatgagca acgacagcca
     1381 gccggaaccg aagcaaagga ccccgaaggg tcacgagata ccgccgccga cgcgaaagca
     1441 ggtccagggc gacctgagta agattccaca agactccttc aggcatggtg gagaatagtt
     1501 ctctcaaatc aggatcatat tgcgggttct gctcgaaaaa ctcggcgacc aagttgggcg
     1561 gctgtgtagg ttcgggggga gtgtttgcgg aagccgagcc tcttgtatcc ggcgtatctg
     1621 aatctccccc tcctcttccg acaacacccc aggcgaccgc gcctcccaca agagatacgg
     1681 ctccggctat tgctcctctc cgagcgacgc ttctcccaag aaggcctctt gtcttatctt
     1741 gaaagccctc ggggttgctc ataactagtc cgccgtgacg ggaagcggct cgcggcctgc
     1801 ggggtcgaga ccgatctgcc cttgctcctc tagtttgcgg tactcctcgt cgctaaaccc
     1861 gagcagcttc ttgtacacgt actcgttgtc ctcgccgagc atcacggggt gccgcttgag
     1921 cttgttcggc gtcttcgaca tcttgtgcat gaggccgggg tatcgatgcg tcccgcactc
     1981 gggatgcgtg agctcctcga agaagccgcg ctcgttcagc tgcggacagg cgaaggcatc
     2041 ggcctcgttc tgcaccggcc ccgacgtgac tccggcggcc tgcaactggt ggaagagctc
     2101 gaagtcgttc ttgtccctcg tccactcgcc gatcgcctcg tccagctcgt cctgatgctt
     2161 gaggcggccg aggctcgtct tgaatcgctc gtcgtgggcc cacgacgggt tgcccgcgac
     2221 gcggcagagc gccgcccact cctcgtcggt cgcgcaatcg atggcgatcc aacggtcgtc
     2281 ccctgcgcag ggatacacgt tgtgcggggc gtggttggga tggcggttcg cctgcggctc
     2341 ccaaacgcgg ccgttcatcg tgtagtcgag gatgatctcg cccaggtacg gcagaaagtt
     2401 ctccgcctgc gccatctcga tgagctgtcc tcgccccgtc cgtgcccgat gccgcagcgc
     2461 catcatgacg gccaccgccc ccatgactcc ggtcgctgca tcgacggtga acgcgtccga
     2521 gaccatcgac ggatcgaggt gccggtacga ccgcaggtag tggtggccgg tcatgccctc
     2581 catgtgcgtg ccgaacgagc gccagttctt gtaggggccg ctcaggccgt acgagggcat
     2641 gcgcagcgag atcagcttct cgttgtaggg gacgagctct tcatgggtga tgtgagcctt
     2701 ctcgatcgtc tccggcacgt tgttctcgat gtagacatcg ctgatggcga tgagccggcg
     2761 gaagatgtcg aggccgccgg gcaccaggac atcgcaggtc atgctgagct tgtcgcgtgc
     2821 atggctattg aagctgggcc cccgattcca cggtcgtggc gccgggtcat cgttggggaa
     2881 ggcggtgagc atgctgttgc tcgacccctt catggcccgg accatctcct tcgtaacggg
     2941 gatctcgccg ccgcgcgtgc tcggctgggt gtggaagagc ggttcgacgc ggatgacctc
     3001 cgcgccccac tccgccagca gctgcgtcac gtacggccca gcccacacca ccgtaacgtc
     3061 gacaacccga attccttcca gtggaagctt gtacgccatg tgttcctccc cctcttgtct
     3121 cgcctatatg gagcctgcgt cgtcctcaga tgacgccctg tgcacggagc ttcgtcaggt
     3181 cctcgtccga atacccgagc ctgccgcaga gcacctcttt cgtgtgctcc ccaaggagcg
     3241 gcgccctgcg agcaagcggc cgcggcgaat cgttcatgat gaacggccgt cccgggtacg
     3301 tgagtggccc tgtggctgga tggtcgatcg tctcgaagaa gccacgctcc cggtagctcg
     3361 gatcgttgag cagatcggcg accgtgttga cgggcgcagc gagcagggtg tgctcctgcg
     3421 cgatcgtggc cgcttccagc ttgccgcgct ccatgagcca tccgagatag ctcgcctcga
     3481 tctcctcgcc caggtcgggg tacatcgcgc ccagcatcat gcccgcatcg gtgcgcggat
     3541 cgctcagcag atcctcgcga ccgatcatgc tgaggaactt cttcatcttc tcgccagacg
     3601 tgacatagat gttcacgtat ccgtccttac agggccggat gcccaacccc gggcgaacgg
     3661 ccgtctccgg ccggcggcct atctcgccgc cgtaggcgtg ggcgacgaga tatgtcgttc
     3721 ggcggtcgcg gaagcccgcc tgcgtttcgt agatggagat gtcgttatgc tggccctcgg
     3781 caccatgcat ctcgacctgc cagagcgcga gcagtgtcgc gtaggcggca acgccgcctg
     3841 cctggtactg cacgacgttg ccgccaagct tgagcgggaa gtgttcgcga tgcccgttga
     3901 cgttcatggg cccgcccatc gcgaactcgg tgatctcgct ggccttgagg tcacggtacg
     3961 gcccggtttg gccgaagttg gagatcgacg tgaggatgat gcgcggattc tgggctgcga
     4021 gcgtctggta gccgaggccg agcgagtcca tcacgcccgg ccggaagttc tccacgacga
     4081 tgtcgaactc ggggagcagc cgctcgacga tgctccggcc ctcggcagac ttgagattga
     4141 gcgtcatgct gcgcttgttc gtgttcagat gcaggaagag ggcgctcttc tccggatgcg
     4201 ggtcgtcctt gtggaacggc ccgtactggc gggagggatc gccgccgggc ggccgctcta
     4261 tcttcagcac gtccgctccc cagtccgcca ggatcttcgt acagtgcgga ccggcgatgt
     4321 gatgggtgag atcgaggact cggacacctt cgagcggagt
//
LOCUS       DATMBB010000078         6969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_584308, whole genome shotgun sequence.
ACCESSION   DATMBB010000078 DATMBB010000000
VERSION     DATMBB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584308"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VNL92_01645"
     CDS             <1..719
                     /locus_tag="VNL92_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93445.1"
                     /translation="GLSAGAALALIHAFLVVTRGANQLASGLALMFFGLGVTAFFGRP
                     YVGEQIDGLPAHPIPGLADLPYLGPVLFDHDLLTYLVFPLGPILWWFLFRSRWGLHLR
                     SVGESRELAFAAGLHPDRIQYLAVAAGGALAGIGGAQLSLGYTQTWIEGMTNGRGFIA
                     VALVIFATWNPLRGMVGALLFGGAVALQLQLQARGADISPFFLDMAPYLLTLVVLLAI
                     GRFGREVMPEGLKSVFRGTG"
     gene            785..1942
                     /locus_tag="VNL92_01650"
     CDS             785..1942
                     /locus_tag="VNL92_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020617111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWO93446.1"
                     /translation="MPSIPGRLRWLLIVLALLATSAFAAACGDDDDDDDDAAAPIQGE
                     ATEPSGDAASEKPTVGFLYVGAVDDGGYNQAAYQGEEALKELGYETISAENVPESAEA
                     ERVMEQMIEQGATIIFPTSFGHLDPAINVAERHPDVTFLHQGGLRTTDNVGTYFGTIW
                     QAEYLAGIAAGRMTESDKLGFIVAFPIPQTLLNINAFHLGARSVNPDVTTTVVFTGNW
                     CDPAKNTEATNSLVNQGIDVVTQHQDCPVPVIQAAEGAGIMSIGYHVDDAQFAPEGWI
                     TAAIWDWTGLFPELVEQVIDGSYEPSQLRLGLADGVVKLAQFGPNVPQEVQDEVMAAQ
                     GQMLSGELFAFTGPISDQDGEVRIAEGERPDVTQLEQIDWLAEGVVGSIPD"
     gene            2019..3032
                     /locus_tag="VNL92_01655"
     CDS             2019..3032
                     /locus_tag="VNL92_01655"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HWO93447.1"
                     /translation="MSGLGGLNKSPDGVVLGLVQLQLPVIEKRDDIAAQCRRIVEMVR
                     KARRSMPTLDLVVFPEYALHGLSMSTAAELMCAIDGPEVEQFREACRAEGLWGCFSIM
                     EANPRGNPYNSALIIDDAGELRLHYRKLHPWVPVEPWEPGDLGVPTCEGPNGSTLAVI
                     ICHDGMFPEMGRECAYKGAEIMIRTAGYTAPIKHAWRITNQANAFCNLMYTASVCMAG
                     TDGAFNSMGEGMIVGFDGEPLVTGNNTPDEIITGEVRPGLVREARRLWGVENNIYQLG
                     HRGYVAVAGGATDCPYTYVRDLARGKYRVPWEDEVTVTDGTSFGFPKPTRRYGELPRR
                     RAG"
     gene            3053..3601
                     /locus_tag="VNL92_01660"
     CDS             3053..3601
                     /locus_tag="VNL92_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HWO93448.1"
                     /translation="MNGTTVNARPYPWPFHGRLQPARVALLTCEDAAAGTHADRDGPL
                     PRLRTVVETIRARGGTIVWLPNRNAAAGAMLACTTTGPSDITVGRPVLSGFTGTGLDL
                     ILRARGITDIVLAGFPLELGADCTMREANDLGYECLMLTDCSSALSPETAAAAIRSIQ
                     MSGGIFGAVAGSDELLRALGAE"
     gene            3629..4339
                     /locus_tag="VNL92_01665"
     CDS             3629..4339
                     /locus_tag="VNL92_01665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWO93449.1"
                     /translation="MSARVAAQPYPWPYDGDIEPARCALLLIDWQHDFCGPGGYVDAM
                     GYDIALTRAGIEPTRRVLELCRGIPNMTILHTREGHKPDLSDCPPNKLWRSRQIGAGI
                     GEPGPCGRILVRGEPGWEIVEELAPLPGEPVIDKPTKGAFGATDIDLVLRNRGITHLI
                     FTGITTDVCVHTIMREANDRGYECLLLSDCTGATYYENYLAAVKMVTMQNGVFGAVAT
                     SEEFMEAVAPMVRAGSLI"
     gene            4336..5805
                     /locus_tag="VNL92_01670"
     CDS             4336..5805
                     /locus_tag="VNL92_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWO93450.1"
                     /translation="MTAAFPSTSTMFRDALHSVRAFPLPVPEPAPLFSLPPTRLAGTR
                     AEAGQPTGPPDDAWATRDAILHRSTSVAEVVARCKQRIAAHSARLGAFEIISDVDAEA
                     ALLEHEAYRGRALGDLHGVPISVKDVIDVAGLPTTGSSAALPARFPSADATAVARLRA
                     AGALIMGKTMTHEFALGVTSPASRNPWDETRVPGGSSGGSAISILTGMAFASLATDTR
                     ASVRIPAALTGGVGLCPSPGLVPRDGWITLSWSMDDLAPMAKSVRDIALLMDVLTGGG
                     TTFRSGLPADAKGLRLAYADPFLDGAAPGVRDVFQRGLDVAAQAGAAIVRSSAPTANE
                     LALANAVGMVISRAEARQYHVESGTDTGRCTPEVREQLEEAAHVTAADYIRALRLRAW
                     LYERLTAPFDETDFLIMPTSKVVAPPRAQADGYLLLLSENAVPWSLVGLPAISIYAGQ
                     AEGLPVGLEIVGRQGEDSRLLGAAYALERVLPPMPAWAP"
     gene            5793..6176
                     /locus_tag="VNL92_01675"
     CDS             5793..6176
                     /locus_tag="VNL92_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWO93451.1"
                     /translation="MGAVMDEAAEAIGRIMGLTPDEARATLVSMYGTDIADEVLAIGR
                     VWNRPGLTRRERSLVVIAALSALGTLARLRIHVGGALHHGATREEIEEVIFTMMAYAG
                     HARATEAIEVARAVFEGSGDPRDSL"
     gene            6561..>6969
                     /locus_tag="VNL92_01680"
     CDS             6561..>6969
                     /locus_tag="VNL92_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HWO93452.1"
                     /translation="MKALVVFESMFGNNQRIAEAIAQGLSEHMAVEVLEVGTAPTSLA
                     DDVALLVVGAPTHMLGMSQPSSRKRAAGQSDRPVVSSGIGVREWLESLNVPPRSTAGA
                     AFDTRLSTRWLRPFPSAAGKIDKGLRKRGIQTML"
BASE COUNT         1214 a         2265 c         2293 g         1197 t
ORIGIN      
        1 cgggcctctc ggctggggcc gcgctcgcgc tcattcacgc cttccttgtg gtgacccggg
       61 gcgctaatca gctagccagc gggctcgcgc tcatgttctt cgggctcggc gtgacggcgt
      121 tcttcggtcg tccatacgtc ggagagcaga ttgacggtct gcccgcgcac ccgattccgg
      181 ggttggcgga cttgccctat cttgggccgg tcctcttcga ccacgacctc ctgacctatc
      241 tagtgtttcc gctcggcccc atcttgtggt ggttcctctt ccgcagtcgg tgggggttgc
      301 atctgcggtc ggtgggagag agccgggaac tcgccttcgc ggccggactc catccggatc
      361 gcatccagta tctcgccgtc gccgcgggcg gggcgctcgc cggcatcggt ggcgctcagc
      421 tttcgctggg ctatacgcag acgtggatcg agggcatgac gaacgggcgt ggcttcatcg
      481 ccgtcgccct cgtcatcttc gccacgtgga atccgctgcg gggaatggtg ggggcgctgc
      541 tcttcggcgg cgccgtggcg ctgcagcttc agttgcaggc gcgcggcgct gacatctcgc
      601 ccttcttcct cgacatggct ccgtatctgc tgacgttggt cgtgttgctg gcgatcggac
      661 ggttcgggcg ggaggtgatg ccggaaggat tgaaatccgt attccggggc acaggatagg
      721 cctgtccccc caagcatagt ccccgtggct gcgacctcac ggaacaggag gagaagacag
      781 acctatgcca agtataccag gccgtctgcg ctggctgctc atcgtgcttg ccctgcttgc
      841 gacttcggcg ttcgccgccg cctgtggcga cgacgatgat gacgatgatg acgccgcagc
      901 gcccatacaa ggcgaggcga cggagccttc gggagacgcc gcatcggaga agcctaccgt
      961 cggctttctt tacgtcggtg ccgtcgatga cggcgggtac aaccaagcgg cgtaccaagg
     1021 tgaggaggcg ctcaaggagc tgggctacga gaccatcagc gcggagaacg tccccgaatc
     1081 cgcagaggcg gagcgtgtga tggagcagat gatcgagcaa ggcgcaacca tcatcttccc
     1141 gacgagcttc ggacatctgg acccggcgat caacgtggcc gagcgccacc cggacgtgac
     1201 gttcctccac caaggtgggc tgcggacaac ggacaacgtc ggcacctact tcggcaccat
     1261 ctggcaagcc gagtacttgg ccgggatcgc tgccggcagg atgacagaga gcgacaagct
     1321 cggcttcatc gtcgcattcc caatcccgca gaccctattg aacatcaatg ccttccacct
     1381 tggcgcgcgc tccgtcaacc ccgacgtgac aaccaccgtc gttttcacgg gcaactggtg
     1441 cgatcctgcg aaaaacaccg aggcgacgaa cagcctcgtg aaccagggca ttgatgttgt
     1501 cactcagcac caggactgtc cggtaccggt gattcaggcg gcagagggcg caggcatcat
     1561 gtccattggc taccatgtcg acgatgccca gttcgcgccg gaaggctgga tcaccgccgc
     1621 tatctgggac tggacaggcc tgttcccgga gctcgtcgaa caggtcatcg acggcagcta
     1681 tgagccgagc caactgcgac tcggtctcgc ggacggggtc gtgaagctag cccagttcgg
     1741 ccccaatgtc ccgcaggaag tacaggacga ggtgatggcc gcgcaggggc aaatgctaag
     1801 cggcgagctg ttcgcgttca ccgggcccat ctccgatcag gatggtgagg tccgcatcgc
     1861 ggagggcgag aggcccgacg tcacccagct cgagcagatc gactggctcg cggagggcgt
     1921 tgtcggcagc attccagact agtttgggtg gctggaccag tggagagaga gccctcctcc
     1981 actggtccag cgattactcg atcgggagca ggttaaccat gtcaggactc ggtggtctta
     2041 acaaatcgcc ggatggtgta gtactagggc tcgtgcaact ccagctgccc gtcatcgaaa
     2101 agcgtgacga catcgccgca caatgccgcc gcatcgtcga gatggtgcgg aaggcgcggc
     2161 gcagcatgcc cacgctcgac ctcgtcgtgt tcccagagta cgcgctgcac ggcctctcga
     2221 tgagcaccgc cgcggagctc atgtgtgcca tcgacgggcc cgaggtagag cagtttcgag
     2281 aggcatgccg cgccgagggc ctgtggggat gcttctccat catggaggcg aaccctcgcg
     2341 ggaatccgta caactcagcc ctcatcatcg acgacgccgg cgaacttcgc ctgcactatc
     2401 gcaaactgca cccatgggtg cccgtcgagc cctgggagcc aggcgatctg ggcgtgccca
     2461 cctgcgaagg gcccaacggc agtacactgg ccgtcatcat ctgtcacgat ggcatgtttc
     2521 ccgaaatggg gcgcgaatgc gcctacaagg gcgcagagat catgatccgc acggccgggt
     2581 acacggcgcc catcaagcac gcctggcgga tcacgaacca agcgaacgca ttctgcaacc
     2641 tgatgtacac cgcctcggtg tgcatggcgg gcacggacgg cgccttcaac tcgatgggtg
     2701 aaggcatgat tgtggggttc gatggcgagc cgctcgtcac cggaaacaac accccggacg
     2761 agatcatcac gggagaggtg cggccaggct tggttcggga ggctcgccgg ctctggggcg
     2821 tggagaacaa tatctaccag ctagggcatc gtggctacgt cgccgttgca ggcggcgcga
     2881 ccgattgtcc gtatacctac gtgcgcgacc ttgcccgcgg taagtatcga gttccgtggg
     2941 aagatgaggt cactgtcacg gacggcacca gctttggctt cccgaaaccg acacgccgct
     3001 acggcgagct gcctcggcgc cgcgccggct gagccccgga aggtcatgaa cgatgaacgg
     3061 gacaaccgtc aacgcgcgac cctacccgtg gccctttcac ggccggctcc agcccgcacg
     3121 cgtcgccttg ctcacgtgtg aggacgccgc cgcaggaacg cacgccgacc gagacggacc
     3181 gctaccgcgc ctccgcactg tagttgagac gatccgggcc cgcggaggca cgatcgtatg
     3241 gctcccgaat cgcaacgcgg ctgcgggtgc gatgttggcg tgcaccacga ccggcccgag
     3301 cgacatcacg gtcgggcggc ccgtcttgag cggcttcacc ggaaccggcc tcgatctcat
     3361 cctgcgcgcg cggggcatca ccgatatcgt gctggccggc ttcccgctcg aactcggcgc
     3421 agactgcacg atgcgtgaag cgaacgacct cggatacgag tgcctcatgc tcaccgactg
     3481 ttcatcggcg ctctcaccgg agacggcagc ggccgcaatc cggtcaatcc aaatgtcggg
     3541 cggcatcttc ggcgccgttg ccggaagcga cgaacttctg cgggcgctgg gcgccgaata
     3601 agcatctcga gacgaaggag gattcaccat gtccgcgcga gttgcggccc aaccgtaccc
     3661 ctggccctac gacggtgaca tcgagccagc gcgctgcgcc ctgctcctca tcgattggca
     3721 acacgacttc tgtggaccgg gcggctacgt tgacgccatg ggctacgaca tcgcgctgac
     3781 gcgagcgggc atcgaaccga cccggcgcgt gctcgaactg tgtcgcggga tccccaacat
     3841 gacgatcctg catacccgcg aggggcataa gcccgacctc tccgactgcc cgccgaacaa
     3901 gctttggcgc tcacggcaga ttggcgcggg catcggagag cctggcccct gcggtcgaat
     3961 cctcgttcgc ggcgagccgg gatgggagat cgtcgaggag cttgcccctc tgccagggga
     4021 gcctgtcatc gacaagccga ccaaaggggc tttcggcgcc accgacatcg atctcgtcct
     4081 acgcaatcgc ggcatcacgc atctcatctt taccgggatc acaaccgatg tctgcgtcca
     4141 taccatcatg cgagaggcga acgaccgtgg ttacgaatgc ctcctgctca gcgattgcac
     4201 gggcgcgacc tactacgaaa actacctagc cgcggtgaag atggtgacga tgcagaatgg
     4261 ggtgttcggg gcggtggcca catccgagga gttcatggaa gccgtggcgc ccatggtccg
     4321 cgcgggcagc ctcatatgac ggccgccttc ccctcgacga gcacgatgtt tcgcgatgcc
     4381 ctccactcgg tgcgggcgtt cccgctgcct gtgcccgaac cggcgccgct gttctcgcta
     4441 ccgccgacac ggctcgcggg cacgcgcgcc gaagccggac agcccaccgg accgccagat
     4501 gatgcatggg caacgcgcga cgccatcctt catcgatcca ccagcgtggc ggaggttgtc
     4561 gcgcgctgca agcaaagaat cgcggcgcac tcagcccggc tgggcgcgtt cgaaatcata
     4621 tcggatgtcg acgcggaagc tgcgctcctc gagcatgagg cctatcgggg cagggcgctg
     4681 ggcgatctgc acggcgtgcc catctctgta aaagacgtga tcgatgtcgc cggcctgccg
     4741 acgacgggca gctccgccgc gctgccggcc cgcttccctt cggccgacgc gacggccgtg
     4801 gcacgccttc gcgccgcggg cgcgctcatc atgggcaaga ccatgacgca tgagttcgcg
     4861 ctgggcgtga cctcacccgc ctcccgcaac ccgtgggacg agacacgcgt gcccgggggt
     4921 tccagtggcg gctccgcgat ttcgatactg accggcatgg cgttcgcctc cctcgcgacc
     4981 gacacgcgag cgtccgtgcg catcccggcc gcgctcaccg gcggcgtagg gctgtgtcct
     5041 tcaccgggcc tcgtcccacg cgacggctgg atcacgctgt catggagcat ggacgatctc
     5101 gcgccgatgg cgaagagcgt acgcgacata gctctcctca tggacgtgct caccggaggg
     5161 ggcacgacct tccggtccgg gttaccggca gacgcgaagg gcttgcgcct tgcctacgcc
     5221 gatcccttcc tcgatggcgc cgcgcccggg gtgcgggacg tcttccagcg agggctcgat
     5281 gtcgcagcgc aggccggtgc agcgatcgtc aggtcctcgg ccccgaccgc gaacgagttg
     5341 gcgctggcga acgcagtcgg catggtcatc agccgggccg aggcacgtca gtaccacgtc
     5401 gagtccggca cggacacggg gcgatgcacg ccggaggttc gcgagcagct cgaggaggcc
     5461 gcccacgtca ccgccgccga ttacatccgg gcattgcgct tgcgggcgtg gctctacgag
     5521 cgtttgaccg cgccgttcga cgaaacggat ttcctcatca tgccaacgtc gaaggtcgtg
     5581 gcgccgcccc gcgcccaggc cgatgggtac ctgctcctgc tttcggaaaa cgccgtgccg
     5641 tggagtctcg tcggcctccc ggcgatcagt atctacgccg gccaggcgga aggcctgccc
     5701 gtcggtttgg aaatcgtcgg acgccaaggc gaggactcgc ggcttctggg cgccgcgtac
     5761 gcgctcgagc gcgtgctccc gcctatgccg gcatgggcgc cgtaatggac gaagcggcag
     5821 aagccatcgg gcgcatcatg gggctgacgc cagacgaggc gcgggcgacc ctcgtctcga
     5881 tgtacgggac ggatatcgcg gacgaggtgt tggccatcgg ccgcgtatgg aaccgtcctg
     5941 gtctgacacg acgggagcgg agcctcgtcg tcatcgctgc cctgagcgcc ctcggcaccc
     6001 tcgcgcggct ccgcattcac gtagggggcg cgctgcatca cggtgcgaca cgcgaggaga
     6061 tcgaggaggt catctttacc atgatggcct acgcggggca cgcccgcgcc acagaggcga
     6121 tcgaggtggc gcgggcagtg tttgagggaa gcggggatcc acgcgactct ctctgaccgc
     6181 gggcagagcg acgcgtacgt gcctacggtc acgccatctt ccccttccat gctggacaag
     6241 tgcggctcga agcatggaac gcaacgggtg cctgcagagc ctccaaagcc gccgaccgat
     6301 tggaccgtgc cttatcgctg aatggcgaag gaggtcggac tggagcccgg catgtcggtg
     6361 ggttacgagc aggccggaag ctaggcgcgt cgcgacccct cgatagatct ccgcactaga
     6421 catcccgtcg gtcccgtata ttcgcacgcg gtagatcctg cgcctcacgg cactcggcga
     6481 tcgggccagt cggccctcat gggtcgtgaa tcgatgcgcc attatgaggg tcgtccgcag
     6541 cgcggcgatg gaggcttatc gtgaaggcac tcgtcgtatt tgaatcgatg tttgggaaca
     6601 atcagaggat cgccgaagct attgcgcagg ggttgtccga gcatatggcg gtcgaggtcc
     6661 tcgaggtcgg caccgcgccg acctctttgg ccgacgatgt tgccctgctt gtcgtcgggg
     6721 cccctacgca catgttgggc atgagccagc ccagctcgcg caagagggcc gcgggccagt
     6781 ccgataggcc cgtcgtatcg tcgggcatcg gcgtacgaga atggctggag tcgttgaacg
     6841 tacccccaag gagtaccgcc ggcgcggcct tcgatacgcg acttagcacg cgctggctgc
     6901 ggccgtttcc gagcgctgcg gggaagatcg ataaggggtt gcggaagcgt ggaatccaaa
     6961 ctatgctcc
//
LOCUS       DATMBB010000079         2848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_385902, whole genome shotgun sequence.
ACCESSION   DATMBB010000079 DATMBB010000000
VERSION     DATMBB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385902"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VNL92_01685"
     CDS             <1..695
                     /locus_tag="VNL92_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWO93453.1"
                     /translation="VAALLAGVLAAAALGVAQELAMWRPMRAKRAGMLQLLLMSLGLA
                     FVLRNVIQFAWGTQPRSLDANVTSSIDFLGLSIGETELIVVIIAFVVLIVVAAMLRVT
                     SLGRQMRALSDNFDLAETTGIDTGRVVLQTWLLAGALAGLAGILVVASTGSLSPNTGF
                     FLLLSLFAAVVLGGIGNAFGALAGGLVIGLTQEWSTLVIESQWKVAVSFIVLIIVLIV
                     RPQGIFGQERTL"
     gene            692..1702
                     /locus_tag="VNL92_01690"
     CDS             692..1702
                     /locus_tag="VNL92_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWO93454.1"
                     /translation="MTPELPVAITFDFLTAWDFWIGVGILAGIYGIFALGLQLNVGYT
                     GVINFGQAGFMAVGAYAAIIFYVDLDWSYLVALPASALVTVLAGLIIGLPSLRLRSDY
                     FAIATIAFSEIIRDAAQNARELTGGNQGTISIELDDEDGDFFTDPWVNASDWIKDNLL
                     DPIGLTGQDFGDLPLFLVVWVTLGLLALGLHFLIRSPWGRVLRAIREDEDAARALGKN
                     AFSYKLQSLAIAAVLGAVSGWLLAFNLESVSQDTFEPLYTFLGFAALILGGLASFGGV
                     LVGSAILWTLLEGMRFLDLPLSADKISALRFILVGLILILLMAFRPQGAFGKREEMVL
                     GD"
     gene            1695..2489
                     /locus_tag="VNL92_01695"
     CDS             1695..2489
                     /locus_tag="VNL92_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93455.1"
                     /translation="MTDGPIFRAEGIVKRFGGIRAVNGASMSIGERSITALIGPNGAG
                     KTTFFNLITGFYRPDAGSATFDGRSTVGRPPYAIARMGMVRTFQITKALAAMPVIDNM
                     MLAAPSQPGESLLRGLIAYPASRAREREVREQAMELLEVFSLERLADEYAGTLSGGQR
                     KLLELARALMTRPRFLLLDEPMAGINPTLGRRLLDHMQRLRAEEGVTFLFIEHDMDVV
                     MNHSDRVVVMAEGRVIAEGEPHEVRSNQAVIDAYLGAVPVEDDGDG"
     gene            2482..>2848
                     /locus_tag="VNL92_01700"
     CDS             2482..>2848
                     /locus_tag="VNL92_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWO93456.1"
                     /translation="MAEPVLTVSGLVAGYTPEVDILTGVDIEVTPNEIVTIVGPNGAG
                     KSTLMKSIFGLVPPRKGSIKLHGDDITGEMAHTVTRKGVSYVPQLDNVFPNLTVQENL
                     ELGAIAGGDGTVAERIKRVF"
BASE COUNT          432 a          949 c          923 g          544 t
ORIGIN      
        1 tcgtcgcggc gctcctggcg ggcgtgctcg ccgccgcagc cctcggagtc gcccaggagc
       61 tcgcgatgtg gcggccgatg cgcgccaagc gggccgggat gctgcagcta ctgctgatgt
      121 cgctcgggct cgcgttcgtc ctccggaacg tgatccagtt cgcctggggc acgcaaccgc
      181 ggtcccttga cgcgaacgtc acctcgtcca tcgacttcct cggcctcagc atcggcgaga
      241 ccgagctgat cgtcgtgatc atcgcgttcg tggtgctcat cgtcgtcgcg gcgatgctca
      301 gggtcacctc gcttgggcgt cagatgcgcg ccctctcgga caacttcgac ctcgccgaga
      361 ccaccgggat cgacaccggc agggtcgtgc tccagacctg gctgctcgcc ggggcgcttg
      421 ccggtctggc ggggatcctc gtcgtggcgt caaccggaag cctctcgccg aacaccggct
      481 tcttcctgct gctctcgctg ttcgccgccg tggtcctcgg aggcatcggc aacgcattcg
      541 gagcgctcgc gggcggactc gtcatcgggc tcacccagga gtggtccacg ctcgtgatcg
      601 aatcgcagtg gaaggtggcg gtcagcttca tcgtcctcat catcgtgctg atcgtccgcc
      661 cgcaaggcat cttcggacag gagcggacgc tgtgacgccg gagcttcccg tcgccatcac
      721 gttcgacttc ctgacggcgt gggacttctg gattggcgtc gggatcctcg ccgggatcta
      781 cgggatcttc gcgctcggcc tgcaactcaa cgtcggctac accggcgtca tcaacttcgg
      841 gcaggccggc ttcatggcgg tcggcgccta tgccgcgatc atcttctacg tcgatctcga
      901 ctggagctac ctggtcgcgc tccccgcttc cgcgctcgtg acggtcctcg cgggcctcat
      961 catcggcctt ccctccctgc gtctgcgctc cgactatttc gcgatcgcga cgatcgcgtt
     1021 ctccgagatc atccgtgacg cggcgcagaa cgcccgcgag ctgaccggcg ggaatcaggg
     1081 cacgatcagc atcgagctcg acgatgaaga cggcgatttc ttcacggacc cttgggtcaa
     1141 tgcatcggat tggatcaagg acaacctgct cgatccgatc ggcctcacgg gccaggactt
     1201 cggagatttg ccactgttcc tggtggtctg ggtcacgctg gggctcctcg cgctcggcct
     1261 tcacttcctc attcgctcgc cctggggccg cgttctgcga gcgattcgcg aggacgagga
     1321 cgcggcgcgg gcgctcggca agaacgcgtt ctcctacaaa ctccagtccc tggcgatcgc
     1381 cgccgtcctt ggtgcggtct ccggctggct tctggcgttc aaccttgagt cggtgtcgca
     1441 ggacaccttt gagccgctct acacgttctt gggctttgca gcgctcatcc tcggcggcct
     1501 ggctagcttc ggtggcgttc tggtcggctc ggcaatcctc tggactctgc tcgaggggat
     1561 gcgcttcctc gacctgccgc tctcagcgga caagatctcg gctctccggt tcatcttggt
     1621 cggcctgatc ctgatcctgc tgatggcgtt ccggccccag ggagcgttcg ggaagcgtga
     1681 ggagatggtg ctcggtgact gacggcccga tcttccgagc cgagggcatc gtcaagcgat
     1741 tcggtgggat ccgcgccgtc aacggcgcgt cgatgagcat cggcgagcgg tcaatcacgg
     1801 cgctgatcgg ccccaacggc gccggcaaga ccacgttctt caacctgatc accggcttct
     1861 accgcccgga cgcaggcagc gcgacgttcg acggcaggtc taccgtcggg cgcccgccgt
     1921 acgcgatcgc gcgaatgggg atggttcgca cattccagat cacgaaggcg ctcgccgcga
     1981 tgccagtcat tgacaacatg atgctcgcag cgccgtccca gcccggcgaa tcgctgcttc
     2041 gggggctcat cgcctatccg gccagccgcg cgcgcgaacg cgaggtcagg gagcaggcga
     2101 tggagttgct tgaggtcttc agcctggagc gcctcgcgga tgagtacgcc gggaccctct
     2161 ccgggggcca gcgcaagctg ctcgagctcg ccagggcgct gatgacgcgt cccagattcc
     2221 tcctcctcga cgagcccatg gccgggatta atcccaccct ggggcggcgc ctcctcgacc
     2281 acatgcagcg ccttcgtgcc gaggagggtg tgacgttcct cttcatcgag cacgacatgg
     2341 acgtcgtcat gaaccattca gaccgcgtgg tggtgatggc cgagggccgc gtgatcgccg
     2401 agggcgaacc gcacgaggtg cgctcgaacc aagcggtcat cgacgcgtat ctcggcgcgg
     2461 ttccggtgga ggatgacggc gatggctgag ccggtcctga ccgtgtccgg gctcgtcgcc
     2521 ggctacaccc cggaggtgga cattcttacc ggcgtggaca tcgaggtgac cccaaacgag
     2581 atcgtcacga tcgtcgggcc caacggagcc gggaagtcaa ccctcatgaa gagcatcttt
     2641 ggcctcgtgc cgccccgtaa ggggtcgatc aagttgcacg gcgacgacat caccggcgag
     2701 atggcgcaca ccgtgacccg caagggcgtg agctacgtgc cgcagctcga caatgtgttc
     2761 ccgaacctga cggtccagga aaacctcgag ttgggcgcga tcgcaggcgg ggacggcacg
     2821 gttgctgaac gcataaagcg tgtgttcg
//
LOCUS       DATMBB010000080         3772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_464650, whole genome shotgun sequence.
ACCESSION   DATMBB010000080 DATMBB010000000
VERSION     DATMBB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464650"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2149)
                     /locus_tag="VNL92_01705"
     CDS             complement(<1..2149)
                     /locus_tag="VNL92_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWO93457.1"
                     /translation="MRLLNWLTTFSVQRASVILLITFAVAGFGIVSIFQTRTELVPNI
                     DFPSLAVITQYRSAAPADVVDDVTRPIEQRLGSINGIEEMRSVSGDGISVVVLNFDFG
                     TDMDQRESEVGTLLQGFPVPPGATRPQLIRASFNQVPVVQFSISSDELDDSELEALVR
                     QDLLPELASIDGVTSAELHGGSDERVNVSLDPDALNNAGLTASQVAQFIRDTDVSQPA
                     GSVAGSETTLPVRAGHDLESIDALRDLTVPAPGGAVPLNALGSVEVQPAPTQGLVITN
                     GQRAIGVNVVKSEDGNTVDVSNEAVSKAREFAEEHPEVSVETVVDQSEFIEESIGGLL
                     REGIIGGVMAVLIVYLFLGSLRATLVTAVSIPLSILIAMIVMNVQGLTINIFTLGGLT
                     VAVGRVIDDSIVVLENVVRHVQLGERIHRAIVLGTREVASAITSSTLTTVAVFLPIGL
                     VGGIVGQLFFAFALAVAAALLGSLLVAITIIPVLARFFVRRGEVRQSEGDEHGGLGRL
                     QRWYTPILTWALDHRAITLVIAGVTFVGSFAIVPFLPVTFLPEMGEAQFQVSVTPPPG
                     ASQDAVEDIVLAIGEQAEQLDDFDVYQATIGGAGGTFETLGAVFSGRGAGGARMVVRL
                     EDGADVDEARERMRAAMAPIQRETGALIAVERGGGGPPGMSSLEIIVQGDNTEAIASM
                     SDRIVEALSPLDDLANLTTDVRRSSAQLAIQVDP"
     gene            complement(2360..2680)
                     /locus_tag="VNL92_01710"
     CDS             complement(2360..2680)
                     /locus_tag="VNL92_01710"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWO93458.1"
                     /translation="MTYVRISILDPDDAERARVEEIIDEMIRFHAGSPGFISGQRLVP
                     DDETGEVWRVAYWQDEESADHAARHDHIMALRSQLNLLVGERRHEERGFWVAGEPRGP
                     AHGH"
     gene            complement(2846..3058)
                     /locus_tag="VNL92_01715"
     CDS             complement(2846..3058)
                     /locus_tag="VNL92_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93459.1"
                     /translation="MAMESQREACPRCRGLMIMERDWYGSYSTCIACGHVREVITTPP
                     IDLRQEAMDGERRQRRRQPSHGKLRL"
     regulatory      complement(3137..3264)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          739 a         1201 c         1160 g          672 t
ORIGIN      
        1 ccgggtcgac ctgaatcgcg agctgtgcgc tcgagcgccg cacgtcggtc gtcagattcg
       61 cgagatcatc gagcgggctc agtgcctcga caatccggtc gctcatgctc gcgatagcct
      121 cggtgttgtc gccctggaca atgatctcca ggctgctcat cccaggaggt ccaccaccgc
      181 cacgctccac ggcgatgaga gcgcccgtct ctcgttgaat gggcgccatg gcggcgcgca
      241 tgcgctcgcg cgcctcgtcc acgtcggcgc catcttcgag tcgcacgacc atgcgagcgc
      301 caccagcgcc ccgcccgctg aatacggcgc cgagcgtttc gaacgtgcct cccgccccgc
      361 cgatggttgc ctggtacaca tcgaaatcgt ccagctgctc ggcctgctcg ccgatagcga
      421 ggacgatgtc ctccactgcg tcctggctcg cccctggggg cggagtgacc gatacctgaa
      481 actgcgcctc gcccatctcc ggaagaaacg tgacgggcag gaacgggacg atggcgaagc
      541 tccccacgaa cgtcacgccc gcgatgacga gcgtgatcgc acggtgatcg agcgcccatg
      601 tgaggatggg cgtgtaccac ctttgaaggc ggccgaggcc tccgtgctcg tcgccctcgc
      661 tttgccgaac ttcaccccgg cgcacaaaga aacgggcgag caccggaatg atcgtgatgg
      721 ccaccaggag cgaccccagc agtgccgcgg caacggcgag cgcgaaggca aagaagagtt
      781 ggccaacgat gccgcccacg agcccgatgg gcaggaacac ggccaccgtc gtcagggtcg
      841 aagacgtgat cgcgctcgca acttcgcggg tgccgagcac gatggcccga tggatccgct
      901 cgccgagctg gacgtgtcgc accacgttct ccagcacgac gatgctgtca tcaatcacgc
      961 gaccaacggc tacggtgagc ccgcccaggg taaagatatt gatggtgagg ccctggacgt
     1021 tcataacgat catggcgatc aggatggata aaggaatgga gactgccgta acaagggtcg
     1081 cgcgaaggct gccgagaaag aggtagacga tgaggacggc catcacgccc ccgatgatcc
     1141 cttcccgcaa cagcccgcca atcgactcct cgatgaattc ggactgatcg acaacggtct
     1201 cgaccgaaac ctccgggtgc tcctctgcga actcgcgcgc cttcgagacg gcctcgttcg
     1261 agacgtcgac ggtattgcca tcctcagact tgacgacgtt gacgccgatg gcacgttggc
     1321 cattcgttat cacgagcccc tgggtaggag caggttgcac ctcaaccgaa ccgagcgcat
     1381 tcaggggaac cgccccgccc ggggccggga cggtaagatc ccgcagtgca tcgatagatt
     1441 ccaagtcgtg gccggcccgc acagggaggg ttgtttcgct gccggccacc gagcctgccg
     1501 gctgcgaaac atccgtatcg cggatgaact gcgccacttg cgaggcggtc aagccggcgt
     1561 tgttcaacgc atcgggatca agcgatacat tgactcgctc atccgagccg ccgtgcaact
     1621 ccgcagacgt cactccatcg atcgaggcga gctcgggcaa gagatcctgc cggaccagcg
     1681 cctcgagctc gctgtcatcg agttcgtcac tgctgatgct gaactgcacg acagggacct
     1741 gattgaagct ggcgcgaatg agctgcggcc gcgtggctcc gggaggcact gggaatcctt
     1801 gaagcagcgt ccccacctcc gactccctct ggtccatatc agtaccgaaa tcgaagttca
     1861 ggacaacgac ggagatgccg tcgccggaga cggagcgcat ctcctcgatg ccattgatgc
     1921 tgccgagacg ctgttcgatc gggcgcgtca cgtcgtcgac gacgtcggcg ggggcggcag
     1981 atcgatactg ggtgatgacc gcgagcgatg gaaaatcgat gttggggaca agctcagtgc
     2041 gggtttgaaa aatggagacg atgccgaaac cggctacggc gaaggtgatg aggagaatga
     2101 cgcttgcacg ctgaaccgag aacgtcgtga gccagtttag taggcgcatc cacaccgcca
     2161 ggcgggtcgc tgcggccagg gctcacgccc gtccaagcac ccgcgacttc caacttcatc
     2221 tcttaccgga cgcttcaagc agtatatggg gggagaattc gtgacgtcta gcacgaactt
     2281 ttcgtgcccg tgcctgacca agcggcagca gcacgaccgc ctacgaccat gcgagacacg
     2341 gagctaacat cgagggcggc tagtgaccgt gggcggggcc gcgaggctct cccgcgaccc
     2401 aaaagcctcg ctcctcatgc cgacgctcgc cgaccagaag attgagctgg gagcgaagcg
     2461 ccatgatgtg gtcgtgacgc gccgcatgat cagccgactc ttcgtcctgc cagtaggcga
     2521 cgcgccagac ctctcccgtc tcatcatcgg ggacgagacg ctgtcccgag atgaatcccg
     2581 gtgagcccgc atgaaagcgg atcatctcat cgatgatctc ctcaacgcga gcgcgctcag
     2641 catcgtcggg gtcgagaatc gagatgcgga cgtaagtcat aagcggcctc ttctcgaagg
     2701 caacggccgc cgacgggcgg gtccggaaat gtggagcgat ggtagcagaa gggcgctcgt
     2761 tgtcaacgtt cgcgcgctca tccttccaca tcgcgtatgg gaggatgagc gcgcgacgtc
     2821 ctcttcgctg ggatgctacg gaatgctaga ggcgcagctt gccgtgggac ggctgccgcc
     2881 ggcgctgacg acgttctcca tccatggctt cctggcgcaa atcgatgggt ggggtggtga
     2941 tgacctcacg aacatgcccg caggcgatgc atgtactgta ggagccgtac cagtcgcgtt
     3001 ccatgatcat gagtccgcgg caacgcggac aggcttctcg ttgactctcc atcgccatct
     3061 ctctcctcct ccggttggca acaaaaaaga ggctcgcgac cgttgcactg cgatacattc
     3121 gtcgcaggcc acgtatcgcg accctctcaa cgcctccggg gttagctgac gggttcgggt
     3181 cgagaggctg accctactcg cattcgcgag attcacccca cggtctgggt cccccggtcc
     3241 ggtcggattc ggcgtcgata cctaggacgt tcctccggtc ttcgtgtccg cgtcctcgcg
     3301 gatgacttgg cgcttcaccg agctagtccc acaaatcaaa acgctcgcgg accctccgcg
     3361 atacgggtgc ccgcggattt tgtgaaggtg ggaacaggtt accggtgtgg tgaggtcgtg
     3421 tcaagcactt gtgaaaaatc gcacccaccg cctcccagcg ccgcccctgg aacggagcgt
     3481 cgcagccaag tacacgtggt cacaatttcc cttgacttcc ccttttgccc gtgttagatt
     3541 ccgcctcacc gcgcgccaga gaccttctac ggcgagagca aaaggtctct gcatgtagtg
     3601 cgcggaccgc cggggggaag ccgtgcgacg ttacattact cagcgtgtcc ttctcatgat
     3661 cccggtgatc tttcttgtca ccgtgggcac attcctcctc attcgtgtcg tcccaggcga
     3721 tatcgtcgac ctgaagtgcg gcaatcagtg cacggaggaa gaccgcgacg ag
//
LOCUS       DATMBB010000081         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_464604, whole genome shotgun sequence.
ACCESSION   DATMBB010000081 DATMBB010000000
VERSION     DATMBB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464604"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VNL92_01720"
     CDS             complement(<1..1662)
                     /locus_tag="VNL92_01720"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93460.1"
                     /translation="MSSEQPLSPYRVLEIASGAGAFCGRVLADLGADVVKLEPPSGDP
                     MRRQPPLAGDTGIAFLWYNANKRGLTLDLDDPACQERVAGALADFDILIDGNPPGWLA
                     TRGLGPADLRGRHPHLIVTSVTHFGQDGPYRDWHGSSLVDFAIAGSLLRCGVPEAPPC
                     APPYELSYAITGITAASATLLALFERGRSGDGDWIDCSVVEAVQAQGDWSVPNYGASG
                     ATQKRAGAGPLFRLYRAADGWVRVINLSPKQWRSFRAWLGDPPEVAGPEWENPLYRGA
                     NQSILDEICNRKFAGRTKADLFFDGQRAGVGIVPIYDPAEVMADAHFRERGTFVPFAT
                     PAGEARAPGAFVRMADHPPGSPRPAPSPGADESVLAGPRAAAGGQGGDGRLPLAGLRV
                     IEVGSGAVAPEITRILGEFGADVIKLESMMQVDFMRLMGQPGSVEASPGYASSNRSKR
                     SLALNLKHPEANRLAQALADKADVIVENNTAGVMSRLGLDYPTLAARNPRLIYLSSQA
                     FGATGPASSFGGFGPTNQAISATTYLWNHPDCDEPQGTQVIHPDHL"
     gene            1823..>2089
                     /locus_tag="VNL92_01725"
     CDS             1823..>2089
                     /locus_tag="VNL92_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO93461.1"
                     /translation="MVQEESIVTPEARAMIGKETGSAQGVVYLKEAQRWAAAVGDLNP
                     IYWDEEAAKAQGYRTIPIPPMFLPVVLAGVTRLDSLREDGIPMRR"
BASE COUNT          338 a          678 c          739 g          334 t
ORIGIN      
        1 cagatggtcg ggatggatca cctgcgtacc ttgcggctcg tcgcaatccg gatgattcca
       61 aaggtaggtc gtggcgctga tggcctggtt cgtagggccg aagcctccaa agctgctcgc
      121 ggggcccgtc gcgccgaagg cttggctgct gagatagatg agccgagggt tgcgggcggc
      181 gagcgtggga tagtcgagcc cgaggcggct catcacgccc gccgtgttgt tctcgacgat
      241 gacatcggcc ttgtcggcca gcgcctgtgc cagccggttt gcttcgggat gctttaggtt
      301 gagcgccaag ctccgcttgc tcctattcga ggaggcgtaa ccggggctgg cttcgaccga
      361 tcccggctgg cccatgagcc gcatgaaatc gacctgcatc atcgattcga gcttgatcac
      421 gtccgcaccg aactccccaa ggatgcgcgt gatctcgggc gcgacggccc cggagccgac
      481 ctcgatgacg cggaggccag cgagtggaag cctgccgtcc ccaccctgac cgccagcggc
      541 tgcgcgaggt cctgccagga cgctctcgtc ggcgcccggc gacggggcag ggcgtgggga
      601 cccgggcggg tgatcggcca tacggacgaa ggcgcccggc gcccgcgctt cgcccgctgg
      661 ggtggcgaag gggacgaacg tgccgcgttc gcggaagtgg gcgtcggcca ttacctcggc
      721 ggggtcgtag atggggacga tgccgacgcc tgcgcgctgg ccatcgaaga acagatcggc
      781 cttcgtgcga ccagcgaact tacgattgca gatctcgtcg aggatgcttt ggttggcgcc
      841 tcggtagagc gggttttccc attccggccc cgctacttcg ggcggatcgc cgagccaggc
      901 gcggaacgag cgccactgct ttggcgatag gttgatgacg cgcacccagc catcggctgc
      961 ccgataaagg cggaacagcg gccccgcacc ggctcgcttc tgcgtcgcgc cgctggcgcc
     1021 gtagttgggc accgaccagt cgccctgcgc ctgcacggcc tccacgaccg agcaatcgat
     1081 ccagtcgccg tcgccgctcc ggccgcgctc gaagagcgcg aggagcgtcg cgctcgctgc
     1141 cgtgatgccg gtaatcgcat acgatagctc gtaaggcggc gcgcagggcg gcgcctccgg
     1201 gacgccgcaa cgcagcaatg agcccgctat cgcaaagtcg acgagcgagg acccgtgcca
     1261 atcgcgatac ggcccgtcct gcccgaaatg cgtcacggac gtcacgatga ggtgagggtg
     1321 ccggccgcgc aggtccgcgg ggccaaggcc tcgcgtggcc aaccagccgg gcgggttgcc
     1381 gtcgatgagg atgtcgaaat cggcgagcgc gccggcgact cgctcctggc acgcggggtc
     1441 gtcgagatcg agcgtgagtc cccgcttgtt cgcgttgtac cagaggaacg cgatgccggt
     1501 atcgccggcg aggggcggtt gccggcgcat tgggtcgccg gaaggcggtt cgagcttgac
     1561 gacatccgcg cccaggtcgg ccagcactct gccgcagaag gcgccggcgc cggaggcaat
     1621 ctccaagact cgatatgggc tgagcggctg ctccgagctc acgcgttccc cctcactcgc
     1681 gccccaaggg ctcgcgcccc ttggctgccg agatatagca cgagtcccga gtccggcgca
     1741 aggtgaggac gtagcttgac agcgccacgg gcccgtgcca gcattcgccc gctttgtggg
     1801 ccttcagctc gaggaggggg acatggttca ggaagaatcg atcgtcacgc ccgaagcccg
     1861 agcgatgatc ggcaaggaga caggttcggc ccagggcgtc gtgtatctga aggaggccca
     1921 acgctgggca gccgccgtcg gcgatctcaa tcctatctac tgggacgagg aggcggcgaa
     1981 agcgcagggg taccggacca tccccatccc tcccatgttc ctgccggtgg tcctcgcagg
     2041 cgtcaccagg ctggatagcc tgcgggagga tggtatcccg atgcggcgg
//
LOCUS       DATMBB010000082         6426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_195055, whole genome shotgun sequence.
ACCESSION   DATMBB010000082 DATMBB010000000
VERSION     DATMBB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195055"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..2036
                     /gene="selB"
                     /locus_tag="VNL92_01730"
     CDS             147..2036
                     /gene="selB"
                     /locus_tag="VNL92_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HWO93462.1"
                     /translation="MYVIGTAGHVDHGKSTLVQALTGIDPDRLREEKERGLTIDLGFA
                     WLKLPDGREVSIVDVPGHERFVRNMLAGAGGVDLALLVVAADEGVMPQTREHLAILDL
                     LGVSHGVVALTKRDLVDDELAELVALDVRETLAGTALADAPLVACSATTGEGLTQLVD
                     VLSAALDTTPRKRDLGRPRLPIDRAFTISGFGTVVTGTLIDGELAVGDMVELHPGGAR
                     ARIRGLQMHRAKADRASPGNRVAVNLSGLAPTDIERGMVLASPGWLRPTNAIGVRLRA
                     VPDLPRPLRHNARVTFHSGCAECPGTVRLLEDDELLPGDEAWAQVRLDREVALVRGDR
                     FVLRVAGETVGGGAIVEVHAPRRRRRHQPTLAALEAHATGPREAVLGIAQSRPFITLA
                     QLASQVELAVDDVVATVRSAIDAGLAVALPLDHPVGPGSMVVATETVDALAGRASQVL
                     ADFHASQPLRSGMPREELRSRLRLDARPFAAILPLFEARGVVRTEGANIGLASFEPRL
                     TAEQDERVRGFLQRLREQPFASPPGDVAEAELLAYLEARGDIVLVDGIAFDAGVHRRI
                     VDEIIEMIHVSGRVSLAEVRDHFKTSRRYAQTILQDLDRRRITRRVGDERVLASEGAA
                     AQVRA"
     gene            2033..3274
                     /locus_tag="VNL92_01735"
     CDS             2033..3274
                     /locus_tag="VNL92_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HWO93463.1"
                     /translation="MTLPLERLAESLPRIRDEALFERTRAVERRANALSALRAASDVE
                     AVTQRVEDAITSWLVAVPLEPPTTRHPRPPSPGVYTILAVDGSHAELDRFAATSCAVI
                     NLGGWRIQYGRNAQAELLRDLRVLTSQDLDCAERDDASRVEMLSGQPLGALRTVEELR
                     FLEQAAAEALSEPDSHPVVGLLDGSLVLWSVSTRTQAARRRIIDEGVIPALNKLRQLA
                     QLYPFAFCGYISHPGSTEVVNMLRVQPNVCYRPSNDRVECQRCEQRADDGSRPCDLVA
                     INDRALFAALLAPGERSAAFRSVSKNKDSVQNQSYEPAGHAVAFCYVRVGVDEVARLE
                     FPLWLAAQPGALDLLHAAVLDQCERGPGYPIALQEAHELAVLTGPDRMTIERLIEDAL
                     QASGVPTSVAGKRGSKRWRMI"
     gene            3259..3870
                     /locus_tag="VNL92_01740"
     CDS             3259..3870
                     /locus_tag="VNL92_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93464.1"
                     /translation="MANDMTATTASLGQIIESSLDRFTFALYSDASTPMLGTPVIARP
                     AGAREVCVYGVVVGIDVGGIDGSRGIDLRRREDEGHEPALSANPHLSYILRRSCETLV
                     IAHEADRRIFAFLPPTPPLPWDGVVAGDDHALRALAADLDVLRPLVNAGPDRDDATAA
                     FVRLLSSAHTDARTFRVHAGKALARLLASDPPRLTALLRKIRP"
     gene            3867..5531
                     /locus_tag="VNL92_01745"
     CDS             3867..5531
                     /locus_tag="VNL92_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWO93465.1"
                     /translation="MNGDGLGLGTSSAASRLGVVIGGSLKAGLDVRLDDGRSVEQMAV
                     GRYVVVHGQHQRFFGMVTDVLLQTEHAGIVGLPTDSGDDFVREVLAGTAAYGMLRIMP
                     LLALDPAIGEPRPVKTVPAHYATVCEAEQDDIARIFGDEGMNHFRIGSPLDMDVKVCL
                     NYNRFIERSNGVFGKTGTGKTFLTRILLASTIMTSDKRTHESDRAVHLIFDMHNEYGW
                     AGKTEGRSEVKGLKQLYPGRVAVFSLDPESSARRNAAVDGPIEIGYGDIEPQDMAALG
                     ETLNLTDAAVDATYVLQRRFGQKHWVREALTLRSVEDETKDTLAELNVHAGSFANLRR
                     GLDRLVRHAFVREETQAQSVRRVLEHLLGGTSVVLEFGRYGDDLAAYMLVANILTRRI
                     HEEYRKRTEIAAGDESRRPNHLVITIEEAHKFLSRKVASQTIFGEIAREMRKYNVTLL
                     IVDQRPSGIDDEVMSQIGTKFSCLLDDESDIDAVLSGVSGRSELRSVLARLDSRQQAL
                     IFGHAVPMPVVVQTESYGPETYERLTGSQLDGRGLLERLREGAEDW"
     gene            complement(5535..>6426)
                     /gene="secF"
                     /locus_tag="VNL92_01750"
     CDS             complement(5535..>6426)
                     /gene="secF"
                     /locus_tag="VNL92_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWO93466.1"
                     /translation="EELRQALSDFGYQDARVQRTESNEFVVRITGLEEEATAASAEQG
                     GEVIGPAPPAEEQSARAQLEDDLRDRFGSMIDASGSTTNAETGEPVGFQDFSSVSESV
                     SRDIGRNAGFAIGVAAVAILLYISWAFRHMPKPFRYGVAALVALAHDALLVIGAFSIL
                     GKAFDTEINTMFITGLLTVIGFSVHDTIVVFDRIRENIATHPGMPMDEAVNRSLTETL
                     SRSLNTSLTVIVTIVAMLLLGGGGIQSLLLVLLIGITAGTYSSIGVASQILVSWDKGD
                     FGRAIRFLTFRRPAVASEAS"
BASE COUNT         1067 a         2195 c         2104 g         1060 t
ORIGIN      
        1 cgccacgcga gggcttggtc tcccgcgagg ctagctggct gcgcgccgct gctcttccgg
       61 ggtctgccga tggcaggccc cctcgctttt cggcgcggct cgcctatact cgtccttgac
      121 gatcggccgc agaaggcgta gaacttatgt acgtaatcgg caccgctgga cacgtcgatc
      181 acggcaagtc caccctcgtg caggcgctga cgggcatcga ccccgaccgg ttgcgtgagg
      241 agaaggaacg cggcttgacc atcgacctgg gcttcgcttg gctcaagctt cccgacggcc
      301 gcgaggtgag catcgtcgac gttccggggc acgagcgttt cgttcgcaac atgctcgcgg
      361 gcgccggggg agtcgacctc gccctgctgg tcgtcgccgc cgacgagggc gtgatgcccc
      421 agacacggga gcatctcgcc atcctcgacc tcctcggcgt gtcgcacggt gtggtcgccc
      481 tgacgaagcg cgatctcgtc gacgatgagc tcgccgagtt ggtcgcgctc gatgtccggg
      541 aaacgctcgc tggcacggcc ctcgccgatg ctcccctcgt cgcttgttcc gccaccacag
      601 gcgagggtct gacacagctg gtcgacgtcc tctcggcggc gctggacacg acgccgcgga
      661 agcgcgatct gggccgcccg cgccttccca tcgaccgcgc gttcacgatc agcgggttcg
      721 gcaccgtcgt cacgggcacg ctcatcgatg gggagctcgc cgtgggggat atggtcgagc
      781 tgcatcctgg aggagcgagg gcgaggatac gtggcctcca gatgcatcgc gccaaggccg
      841 accgcgcctc accgggcaac cgcgtcgccg tcaacctcag tggtctcgcc ccgacggata
      901 tcgaacgggg gatggtcctg gccagtccag gctggttgcg gcccacgaac gccatcggcg
      961 tgcgcctgcg cgccgtcccc gacctgccgc gcccgctacg acacaacgcc cgcgtcacgt
     1021 tccacagcgg ctgcgcggaa tgtccgggca ccgtgcgact gctggaggac gacgagctgc
     1081 ttccgggcga cgaggcgtgg gcacaggtcc ggctggaccg cgaggtcgcc ctcgtgcggg
     1141 gggaccgttt cgtgcttcgt gtcgccggag agacggtcgg gggcggcgcc atcgtcgagg
     1201 ttcacgctcc gcgccgtcgc cgtcgccacc agccaaccct cgcagcactg gaggcacatg
     1261 caaccggacc ccgggaggcc gtgctcggca tcgcccagag ccggcccttc atcacgctcg
     1321 ctcagctcgc gtcccaggtg gagcttgcgg tcgacgacgt ggtggctacg gtgcgctcgg
     1381 ccatcgatgc tgggctcgcg gttgcgttgc cgctcgacca tcccgtcggg ccgggctcca
     1441 tggtcgttgc gaccgagacg gtcgacgccc tcgccggacg ggcaagccaa gtcctcgccg
     1501 atttccatgc gtcgcagccg ctgcgctccg gcatgccccg cgaggagctc cggtcgcggc
     1561 tgcgcctcga tgcgcgcccc ttcgcagcaa tcctcccgct cttcgaggcc cgcggcgtcg
     1621 tgcggacgga aggcgccaat atcggcctcg catcgttcga gccccgcctg acggccgagc
     1681 aggacgagcg ggtgcgcggc tttctccaac gcctccgcga gcagcccttc gcctcgcccc
     1741 cgggcgacgt ggccgaagcg gagctcctcg cgtacctcga ggcccgcggc gacatcgtgc
     1801 tcgtcgatgg catcgccttc gatgctggcg tccaccgtcg catcgtcgac gagatcatcg
     1861 agatgatcca cgtgagcggc cgcgtcagcc tcgccgaggt gcgtgaccac ttcaagacca
     1921 gccgccgcta tgcccagacg atcctccagg acctggaccg ccgacggatc acccgccggg
     1981 tcggcgacga gcgcgtcctc gcgagcgagg gtgctgccgc ccaggttcgc gcatgaccct
     2041 tccgctcgag cgcctcgccg agtcactgcc gcgcattcgc gacgaagcgc tgttcgagcg
     2101 tacccgcgcc gtcgagcgcc gagcgaacgc actgtccgct ttgcgcgccg catcggatgt
     2161 cgaagcggtc acccagcggg tcgaggacgc gatcacgagc tggctcgtcg cagtgccgtt
     2221 agaaccaccg acgacgcgcc atccgcgtcc cccgtcgccg ggcgtgtaca cgatccttgc
     2281 cgtcgacggg tcgcacgccg agctcgatcg ctttgcggcc accagctgcg cagtgatcaa
     2341 cctcggtggc tggcgcatcc agtacggccg gaatgctcag gcggaactgc tccgcgatct
     2401 gcgggttctc acgagccagg acctcgactg cgcggagcgg gacgacgcta gcagggtcga
     2461 gatgctctcg gggcagcctc tcggggcact ccgcaccgtc gaggagctcc gcttcctcga
     2521 gcaagccgcc gccgaggccc tctcggagcc ggatagtcac ccagtcgtcg gcctgctgga
     2581 tggcagcctc gtcctctggt cggtctccac gcgcacgcag gcggcacggc ggcgcatcat
     2641 cgacgaaggc gtcatccccg cgctgaacaa gttgcggcag ctcgctcaac tctatccctt
     2701 cgcgttctgc ggctacatca gtcatccagg ctccaccgaa gtcgtgaaca tgctccgcgt
     2761 gcagccgaac gtctgctatc gccccagcaa tgatcgcgtg gagtgccagc gttgcgagca
     2821 gcgggctgac gatggttcgc ggccatgcga cctcgtcgcc atcaacgacc gcgccctgtt
     2881 cgcggcgctg ctggcacccg gggaacggag cgcggcgttc cgctccgtct cgaagaacaa
     2941 ggattcggtg cagaaccaat cgtacgagcc ggccggccat gcggtcgcgt tctgctacgt
     3001 tcgagttggc gtcgatgagg tggcgcggct ggagtttccg ctgtggttgg cggcgcagcc
     3061 aggcgcgctc gacctccttc acgccgctgt cctcgaccaa tgcgagcgcg ggccgggcta
     3121 tcccattgcc ctgcaggagg cgcacgagct cgccgtcctc accggccccg acagaatgac
     3181 gatcgaaagg ctcatcgagg atgctttgca ggcatcaggg gtgcccacgt cggtcgcagg
     3241 caaacgaggg agcaagcggt ggcgaatgat atgacggcga cgacagccag cctcgggcag
     3301 atcatcgagt cctcgctcga ccggttcacg ttcgccctgt attccgatgc ctccacgccc
     3361 atgctcggta cgcccgtcat cgcacgcccc gcaggcgctc gggaggtgtg cgtgtatggc
     3421 gtcgtcgtag gcatcgacgt cggcgggatc gatgggagcc gcggaataga tctaaggcga
     3481 cgcgaggacg aagggcacga accggcgctc agcgcgaatc cgcatctctc gtacatcctc
     3541 cgccgatctt gcgagacatt ggtcatcgcc cacgaggcgg atcgtcgcat cttcgcgttc
     3601 ctgccgccaa cgccaccact gccgtgggat ggtgtggtcg ccggtgacga tcacgcactg
     3661 cgtgcgctgg ccgccgatct cgacgtgctc cggccgctgg tcaatgcggg accggatcgc
     3721 gacgacgcta ccgccgcatt cgtacgcctg ctttcgtccg ctcacaccga tgccaggacc
     3781 ttccgtgtgc atgcagggaa ggccctcgcc cggctgctcg ccagcgatcc gccacggctg
     3841 accgcactgc ttcggaagat tcggccatga acggggacgg gctgggattg ggcacaagct
     3901 cggcagcgtc tcgcctcggc gtggtcatcg gtggctcgct gaaagcaggt ctggatgtgc
     3961 gcctggatga tggccggtct gtagagcaga tggccgtcgg ccgctacgtc gtggtgcacg
     4021 gccaacatca gcggttcttc ggcatggtta cggatgtcct gctgcagacc gagcacgcgg
     4081 gcattgtggg cctacccacc gattcgggcg acgacttcgt ccgcgaggtg cttgccggga
     4141 cggcggccta cggcatgctg cgcatcatgc cgctcctcgc gctcgatccg gcaatcggcg
     4201 aaccccggcc ggtcaagacg gtgccggcgc actacgcgac ggtctgcgaa gccgagcaag
     4261 acgatatcgc ccgcattttc ggcgacgagg ggatgaacca cttccgcatc ggctcgcccc
     4321 tcgatatgga cgtgaaggtc tgcctcaact acaaccgctt catcgagcgc tcgaacggcg
     4381 tgttcggcaa gaccggcacg ggcaagacat tcctcactcg catccttctc gccagcacga
     4441 tcatgacctc ggacaagcga acgcatgaga gcgaccgggc cgtccatctc atcttcgaca
     4501 tgcacaacga gtatgggtgg gcaggtaaga cggaaggccg gagcgaggtc aagggcctga
     4561 agcagttgta tcctgggcgc gtcgccgtct tctcgttaga cccagaaagc tcggcccgcc
     4621 gcaacgccgc cgtggatgga cccatcgaga tcgggtacgg cgacatcgag ccgcaggaca
     4681 tggccgccct cggggagacg ctcaacctca ccgacgccgc ggtcgacgcg acgtacgttc
     4741 tgcagcgccg cttcggtcag aagcactggg tacgggaagc cctgaccctc cgctcggtgg
     4801 aggacgaaac gaaggacacg ctcgccgagc tgaacgtgca cgcggggtcg ttcgccaatc
     4861 tccggcgcgg gctggatcgc ctcgtgcgcc atgcatttgt gagggaggag actcaggcgc
     4921 agtccgtgcg gagggtcctg gagcatctgc tggggggaac gagtgtcgtc ctcgagttcg
     4981 gtcgctacgg cgatgatctc gcggcctaca tgctggtcgc caacatcctc acgaggcgca
     5041 tccacgaaga ataccgaaag cgcaccgaaa tcgcggcggg cgatgagagc cggaggccca
     5101 accacctcgt cattaccatc gaggaagcac acaagttcct gagccgcaag gtggcgagtc
     5161 aaaccatctt cggcgagatc gcgcgcgaga tgcgcaagta caacgtcacg ctcctcatcg
     5221 tcgaccagcg cccgagtggc atcgacgacg aggtgatgtc gcagatcggc acgaagttct
     5281 cctgcctcct cgacgatgag agcgacatcg atgctgtcct gtccggcgta tcaggacgct
     5341 cagagctacg gtcggtgctc gcccggctcg acagcaggca gcaggcgctc atcttcggtc
     5401 acgccgtacc gatgccggtc gtcgtccaga ccgaatcata cgggcccgag acctacgagc
     5461 ggctcaccgg cagccaactc gacggccgtg ggttgctgga gcgacttcgc gaaggcgcgg
     5521 aggactggta gaagctagct cgcctcgctc gccacagccg gcctccggaa cgtcaggaag
     5581 cggatggccc ggccgaaatc gcccttgtcc cacgagacaa ggatctgact cgccacgccg
     5641 atcgagctat acgtccccgc cgtgatgccg atgagcagca cgagaagcag cgactgaatc
     5701 ccgcccccgc cgagcagcag catcgcgacg atggtgacga tgaccgtcag cgacgtgttc
     5761 agcgatcgcg agagcgtctc cgtcagcgag cggttcaccg cttcgtccat cggcatgcca
     5821 ggatgcgtcg cgatgttctc tcggatgcga tcgaacacaa cgatcgtgtc gtgcacgctg
     5881 aagccgatca cggtcaggag gcccgtgatg aacatcgtat tgatctcggt gtcgaacgcc
     5941 ttgccgagaa tcgagaacgc gccgatcacc agaagcgcgt cgtgggcaag cgcgacgagg
     6001 gcggccactc cgtagcggaa tggcttgggc atatggcgaa aggcccagct gatgtagagg
     6061 aggatggcga cggccgcgac gccgatggcg aaaccagcgt ttcgtccgat gtcgcgcgag
     6121 accgactcgg agaccgacga gaagtcctgg aatcccaccg gctcgccggt ctccgcattc
     6181 gtcgtgctgc cgctcgcatc gatcatcgac ccgaagcgat cgcgcaggtc gtcctccaat
     6241 tgggcccggg cggactgttc ctcagcaggc ggggcgggac caatcacttc tccgccctgc
     6301 tcggccgaag cagctgtcgc ctcctcctcg aggccggtga tgcggacaac gaattcattg
     6361 gactcggtgc gctgcaccct cgcgtcctgg tagccaaagt ccgacagggc ttgtcgcagt
     6421 tcctcg
//
LOCUS       DATMBB010000083         2529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_507253, whole genome shotgun sequence.
ACCESSION   DATMBB010000083 DATMBB010000000
VERSION     DATMBB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507253"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VNL92_01755"
     CDS             complement(<1..182)
                     /locus_tag="VNL92_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93467.1"
                     /translation="MNGQERWNARYEGWKRRRMSRRTALRGVTIGGVGAAAAATIGCG
                     DDDDDDDDAEAPGAGAS"
     gene            complement(232..1143)
                     /locus_tag="VNL92_01760"
     CDS             complement(232..1143)
                     /locus_tag="VNL92_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93468.1"
                     /translation="MTRSVPEAVLPATSPAEVLAARPSKVVAFWRAFRKKPLGLFGLI
                     VLVVVVAVALTAPLVAPYGRDESVNLFFQSPTWAHPFGTDHLGRDVFSRVIYGASVSL
                     QVGVAAVALGTLVGTAAGVVSGYFGRWVDISLQRVIDVLMALPGILLALTIAAALGAS
                     QRNVTIAIAIALLPASARVARGSTLSVKERQYVEAAHALGAGHVRIMLRHVLPNIMAP
                     LIVIVSVQLGVAIIAEASLSYLGLGVPINVPTWGNMLSGAALSYMTKAPWMAIFPGVA
                     LTLVVLGINALGDALRDLLDPRMRGTQ"
     gene            complement(1151..2101)
                     /locus_tag="VNL92_01765"
     CDS             complement(1151..2101)
                     /locus_tag="VNL92_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93469.1"
                     /translation="MRTYIVRRIVLAALVLLGLSVLVFILLRAVPGDAASMILGTDVS
                     ADAEQRERLRDELGLNDPLPVQYFRWLGDFLQGDLGESLFTGRSVWSEIQDRVPVTVE
                     LGLWAIFFAIVVAIPLGVLSAIRQDSALDQGLRFVSILGLAIPNFWLGTMVVVFGSRW
                     FGWIPPVGYTPIEEDPIRNLEQFVLPGAIIGLAFAASLMRISRSSMLEVLREDFVRTA
                     WAKGLRERTVVVRHVMRNALIPVVTVFSIQLGVVVTGTVIIENIFNLPGMGRLMLDAI
                     ARRDYPLVQGIAMLIGTVIVAVNLVTDLIYAWLDPRIRYG"
     gene            complement(2114..>2529)
                     /locus_tag="VNL92_01770"
     CDS             complement(2114..>2529)
                     /locus_tag="VNL92_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93470.1"
                     /translation="LERYRDVAARKRHEDELDATIAAWTRELDHYDVMHRLQAGGVPA
                     GAALDCAEMFDDSHMSARGQFEWVRHPELGDSPHTRVAWKLRGTPSPVTVPAPCFGQH
                     NDEVLRGLLGMSEPEIARLSEAGVIANEPPPLPGH"
BASE COUNT          488 a          868 c          769 g          404 t
ORIGIN      
        1 gaagcgccgg ccccgggagc ctctgcatcg tcgtcatcgt catcgtcgtc gccacatccg
       61 atcgtggccg ccgcggccgc cccgacgccg ccgatcgtca ccccacggag tgccgtgcgc
      121 cgcgacatcc ggcgtcgttt ccagccctcg tatcgagcgt tccagcgctc ctgtccgttc
      181 atcttggcct ccctccttcg ttgacgacgc ccgccgggac cacattcttg ctcattgagt
      241 gccgcgcatg cggggatcga gcagatcacg cagggcgtcg cccagagcgt tgatgccgag
      301 tacgacgagc gtcagcgcca cccctggaaa gatcgccatc caaggcgcct tcgtcatata
      361 cgaaagggcc gcgccgctca gcatgttgcc ccaagtgggc acgttgatgg ggacaccgag
      421 gcccaggtag ctcaaggaag cctcggcaat gatggcgaca ccgagctgca ccgacacgat
      481 gacgatgagg ggcgccatga tgttcggcag cacgtgacgc aacatgatcc ggacatgtcc
      541 ggctcccaat gcgtgcgcag cttcgacgta ttgccgttcc ttcacactga gcgtactgcc
      601 gcgggcgacg cgagccgacg ccggcagcaa cgcgatggcg atggcgatcg ttacgttgcg
      661 ctggctcgcg ccgagcgctg cggcgatagt cagggccaac aggatgccgg gaagggccat
      721 caacacatcg atgactcgct gtaggcttat gtcgacccac cgtccaaaat agcccgacac
      781 aacgcccgcc gcggtgccaa ccaaggtgcc caacgccacc gccgcaacac caacttggag
      841 cgacacgctc gctccataga tgacccggct aaacacatcg cgcccgagat ggtccgttcc
      901 aaatggatga gcccaggtcg ggctttggaa gaagaggttg acgctttcgt cgcgtccata
      961 gggcgcgaca aggggcgccg tcaacgccac ggctacgacg accacgagca cgatgagccc
     1021 gaacaatcca agtggcttct ttcggaacgc gcgccagaac gccacgacct tgctcgggcg
     1081 ggcggccagc acttccgccg ggctggtcgc gggaaggacg gcctccggaa cgctccgtgt
     1141 catcgccacc tcagccgtat cggattcgcg gatcgagcca cgcgtagatc aagtccgtca
     1201 ccaggttcac cgcgacgatc acggtgccga tgagcatcgc gattccctgc accaatgggt
     1261 agtctcggcg agctatcgca tccagcatca ggcgtcccat ccccgggagg ttgaagatgt
     1321 tctcgatgat gacggtgccg gtcaccacaa ccccgagctg aatcgagaac acggtaacca
     1381 ccggtatgag cgcgttgcgc atcacgtgcc ggacgacgac ggtccgttcc cgcagcccct
     1441 tcgcccaggc agtccggacg aagtcctccc gcagcacctc cagcatggag gaacggctga
     1501 tgcgcatgag cgaagcggcg aacgccaggc cgatgatggc accgggaagt acgaattgct
     1561 ccagattccg gatcggatct tcctcgatcg gcgtgtagcc aaccggcgga atccagccaa
     1621 accaccgcga cccgaacacg acgaccatcg ttcccagcca gaagttcggt attgccaatc
     1681 cgagaatgct gacgaaccgc aacccctggt cgagtgcgga gtcctggcga atcgcggaca
     1741 gcacgccgag cgggatcgcg accacgatcg cgaagaagat ggcccataat cccagctcca
     1801 ccgtgacggg aacgcgatcc tgaatctcgc tccagaccga tcgtcctgtg aatagcgact
     1861 cgcccagatc tccctgcaag aagtcgccca gccatcggaa atactgcacc ggcagcggat
     1921 cgttcagtcc caactcgtcc cgcagccgtt cccgctgctc cgcatcggcc gaaacgtcgg
     1981 tgccaaggat catcgacgcc gcgtcgccgg gtacggcccg aagcaggatg aagacgagca
     2041 cgctgagccc cagcagcacg agggctgcta gcacgatcct tcgcacgatg tacgtccgca
     2101 cgcgacctcc tggctagtgc ccaggcaagg gcggtggctc gttggcgatg acgcccgcct
     2161 cggacagacg ggcgatttct ggttccgaca tgccgaggag gccgcgtagc acttcgtcgt
     2221 tgtgctggcc gaagcacggc gccggcacgg ttaccggcga gggcgtccct ctcagcttcc
     2281 aagcgacgcg cgtgtgcggc gaatcgccga gctctgggtg gcgcacccac tcgaattgcc
     2341 cccgtgcgct catatgggag tcgtcgaaca tctcggcgca gtcgagcgcc gcgcccgcgg
     2401 gtacaccccc ggcctgcagg cgatgcatca cgtcgtagtg gtcgagctcc cgtgtccacg
     2461 cggctatcgt cgcgtcgagc tcgtcctcgt ggcgcttccg cgcggccacg tctcgatagc
     2521 gttcgagcg
//
LOCUS       DATMBB010000084         5978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_430379, whole genome shotgun sequence.
ACCESSION   DATMBB010000084 DATMBB010000000
VERSION     DATMBB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430379"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VNL92_01775"
     CDS             <1..161
                     /locus_tag="VNL92_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWO93471.1"
                     /translation="LRMAMTQLDLSARAFHRTLKLARTIADLDDSDDIGPAHLAEAIQ
                     YRRREIEA"
     gene            244..1062
                     /gene="otsB"
                     /locus_tag="VNL92_01780"
     CDS             244..1062
                     /gene="otsB"
                     /locus_tag="VNL92_01780"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWO93472.1"
                     /translation="MTARRLDEQAGPVVALLSHRPFAWFTDVDGTLSPIVQDPAEATV
                     PPTLRALAERLARRVDHFVVVSGRTPRHAREMVGVRRALYVGNHGLARLERERETLIP
                     EAEPYVAKVARLERELAETLAVEGLVVENKGPILALHYRAAPDPAAARESILAAARVH
                     AAGLGMHVHEGRKVVELRPPINRDKGAAVHEVVSTMGVHGAVYVGDDLTDIDAFRALR
                     ALRETGGLRAISIAAAAPEVDARVLREADFRVEGVEGVERLMAAVVRVLETGER"
     gene            complement(1137..2267)
                     /locus_tag="VNL92_01785"
     CDS             complement(1137..2267)
                     /locus_tag="VNL92_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93473.1"
                     /translation="MDLGLNEQQEMLKNFARDFLEKECPETLVRQMEEDERGYSPELW
                     KKMADQGWHGLIIPEQYGGAGLEFLDLIVLLEEFGRALVPGPFISTVVHAAYPILLAG
                     SEQQKQQYLPKIASGDLIGCLAYTEPSAQFKPDAISLEAKKSGNGYTLNGTKLFVENA
                     NVADIIVVVARTGGSGAQGLSLFVVDAKSPGIEITPLKTIASDKQCEVTFKDVQVPAE
                     NLLGAEGQAAATLEQVQQRATVAECAYLVGLAQMDFEISVNYAKERVQFGRPIGSFQA
                     IQHKAADMVTDVDGARFIMYKAAWSVQEGESDADLNVHMAKAWCSDATRRVVAHGQQI
                     HGGIGFTKDYKIQLFFRRQKKAELMWGDGDFHREYVAEALGV"
     gene            complement(2324..3508)
                     /locus_tag="VNL92_01790"
     CDS             complement(2324..3508)
                     /locus_tag="VNL92_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWO93474.1"
                     /translation="MDFRDTPEQARWRNEVRSFLDAELAPDWRGGGDEEAMFGTGRRE
                     VITAWRKKLAARGWIAPAWPKEYGGASMSIMEQFIFNEEVAKAQAPPNDMIGVGMVGP
                     TLIVYGTEEQKKEHLGGILSGERWWCQLYSEPGAGSDLASLQTRAVRDGDDYVVNGQK
                     IWTSGAHIADYGILLARTDPTAPKHKGISYFMVDMKSPGITVRPLVNMADNHSFNEVF
                     FEDVRVPKKNMIGEENRGWYMAVTTLDFERSAIGGNVGMRRQIDELIAFAKEARADGR
                     STLDANPSLRYELADRVVEAEVAKMMSYRIVTMQDRGLIPNHEASCAKLFSSELSQRV
                     AATGMHVLGLYGQISPKSKWAPLRGRIERSYLGAVSATIAGGTSEIQRNIIAQRGLGM
                     PR"
     gene            complement(3538..4722)
                     /locus_tag="VNL92_01795"
     CDS             complement(3538..4722)
                     /locus_tag="VNL92_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93475.1"
                     /translation="MDFRDTTEEAAFRDEVRAFLRQELPGGWQGETDPREQRADGEER
                     YQQWMKKLASRGWIAPAWPKEYGGAGLSVKEQFVFNEEMARARAPRPGGIGVGMAGPT
                     IIVYGTDEQKQQHLSGILSGEVTWCQLYSEPGAGSDLAALQTRAVRDGDDYVVNGQKI
                     WTSGAHRAQRGILLARTDPDAPKHKGISYFLLDMKTPGISIRPLVNMAGTHEFNEVFF
                     DNVRIPKRDLLGEENRGWYMATTTLDFERSSIGNAVGMEQMVEDLLNFVHANRNEPFM
                     AVGHTPSVRYELADRRIEVAVAMLMSYRIITMQSQGMIPNHEASCAKLFSTELTQRIA
                     RTGLKLIGTYGQLERGSPWAPLRGRFERMYLRSVSATIAGGTSEIQRNIIAMRGLGLP
                     RG"
     gene            complement(4890..>5978)
                     /locus_tag="VNL92_01800"
     CDS             complement(4890..>5978)
                     /locus_tag="VNL92_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWO93476.1"
                     /translation="AYLSVESVTPLNVGPLDGGDDERISLNDGYDPGDGTSFSAPFVA
                     GTAALMLSRNPTLSGIEVMDIIKAAAQDLPDAGEPNWDGAGRLRANVALAAVPATFLG
                     RVTSQGAPAGPGIRIDAYVGEQLCGSTVSTFGNDGSSEYQLDVASAMLQPGCGTEGAT
                     VRITVAGANTAVEAPWNGTVTSIDLETALGTSGTGIELEAGWNLVAFSFTPTTTSIES
                     LLAPLGDAAVSAITFDEAEQVFRFFVPGNPGVSDFTELPPLAGMWLRVSGPVTLVVPD
                     SGVPPPETVELVAGLNLVGLPLNGPVPIAETVASIQGALLSILGFTDGLDGAFLVYLP
                     DGSLATLTELRPNEGYWFVASEATTLRP"
BASE COUNT          955 a         1913 c         2004 g         1106 t
ORIGIN      
        1 tgcttcgcat ggcgatgacg cagctcgacc tctcggcgcg ggcgttccac cgcaccctga
       61 agctcgcgcg aacaatcgcc gacctcgatg acagcgacga catcggtccg gcgcatcttg
      121 cggaggcgat ccagtatcgt cgccgcgaga tcgaggcgta atggcaacac ctggcgaatc
      181 catcgaggcg agggccttgc ccatgaaccg atgcaggcat gacggcaggg gcggcgacaa
      241 ccggtgaccg cacgccgcct cgatgagcag gccggacccg ttgtcgcgct tctctcgcat
      301 cgtcccttcg cgtggttcac ggacgtcgat ggaaccctga gccccatcgt tcaggatccg
      361 gccgaggcca ccgtgccgcc aactctgcgg gcgctcgcgg aacgattggc gcgccgtgtc
      421 gaccacttcg tcgtcgtttc tggtcgcacg cctcgtcacg cgcgcgagat ggtcggggtg
      481 cgacgcgcgc tgtacgtcgg gaaccacggg ctcgcccggc tcgagcgcga acgcgagacg
      541 ctgattccgg aggcggagcc gtatgttgcc aaggtggcgc ggctggaacg agagctagcc
      601 gagacgcttg cggtcgaggg ccttgtcgtc gagaacaagg ggccgatcct cgcgctccac
      661 taccgcgccg cgcccgaccc ggccgccgcc agggagagca tcctggccgc ggcccgtgtg
      721 catgccgcgg ggctcgggat gcacgtgcac gagggacgga aggtcgttga gctgcgaccg
      781 cccatcaatc gggacaaggg cgcggcggtg cacgaggtgg tgagtacgat gggcgtccac
      841 ggggctgtct acgtgggcga cgaccttacg gatatcgatg cgttccgggc gctgcgcgcg
      901 cttcgcgaga cgggcggcct ccgtgcgatc tccattgcgg ctgctgcgcc ggaagtggat
      961 gctcgcgtgc tgcgggaggc agatttccgc gtcgaaggcg tggagggcgt cgagcggctc
     1021 atggccgccg tcgtccgcgt actcgagacg ggcgagcgct aggctaagga ttcgcgagga
     1081 agtcgaatgc ccccgtcgca ggacgggggc attcgggtgt gtcaaaagct tgggcgtcag
     1141 acgccgaggg cctccgcgac gtactcgcgg tggaagtcgc catcgcccca catgagctcg
     1201 gccttcttct ggcgccggaa gaagagctgg atcttgtagt ccttcgtgaa gccgatgccg
     1261 ccgtggatct gctggccgtg ggcgacgacg cgccgcgtgg cgtcgctgca ccacgccttc
     1321 gccatgtgga cgttgaggtc ggcgtcggat tcgccttcct gcaccgacca agccgccttg
     1381 tacatgatga agcgcgcgcc gtcgacatcg gtcaccatgt cggcggcctt gtgttgaatc
     1441 gcctggaagg agccgatcgg ccggccgaac tggacgcgct ccttggcgta attgacgctg
     1501 atctcgaagt ccatctgcgc caggccgacg aggtaggcgc actcggcgac ggttgcccgc
     1561 tgctgcacct gctccagtgt cgccgcggcc tggccctcag cgccaaggag gttctccgct
     1621 ggaacctgca cgtccttgaa ggtcacttcg cactgcttgt cgctagcgat ggtcttgagc
     1681 ggcgtgatct cgatccccgg gctcttcgca tcgacgacga agaggctgag gccctgagct
     1741 ccggagccgc ccgtccgggc gacgacgacg atgatgtcgg ccacgttcgc gttctcaacg
     1801 aagagcttcg tgccattcag cgtgtagccg ttgccggact tcttcgcctc aagcgatatg
     1861 gcatccggct tgaactgcgc cgagggctcg gtgtaagcca gacagccgat gaggtcgccg
     1921 ctggcgatct ttggcaggta ctgctgcttc tgctgctcgc tgccggcgag aaggatgggg
     1981 taggccgcgt gcacgacggt ggagatgaag ggcccgggga cgagcgctcg gccaaactcc
     2041 tcgagaagga cgatgaggtc gaggaattcg aggccggcgc ctccgtactg ctcgggaatg
     2101 atgaggccgt gccatccttg gtcagccatc ttcttccaga gctcgggcga gtaacctcgc
     2161 tcgtcctcct ccatctggcg gaccagcgtt tccgggcact ccttctccag gaagtcgcgc
     2221 gcgaagttct tgagcatctc ctgctgttcg ttgagtccga ggtccatggt tgtctccctc
     2281 tttcgcttcc cccgccgacg ttcgacgtat ggttccctgc cgactagcgc ggcatgccaa
     2341 gcccgcgctg ggcgatgatg ttgcgttgga tctcgctcgt gccgcccgcg atcgtcgctg
     2401 aaaccgctcc aaggtacgag cgttcgatgc gcccgcgcag gggtgcccac ttcgactttg
     2461 gcgagatctg accgtacaac cccagcacgt gcatgccggt ggccgcgacc cgctggctga
     2521 gctcgctgct gaagagcttc gcgcacgagg cctcatgatt cgggatgagg ccccggtcct
     2581 gcatcgtgac gatgcggtag ctcatcatct tcgcaacttc cgcctccacg acgcgatctg
     2641 cgagctcata gcggaggctt gggttggcgt cgagcgtgct ccggccgtcg gcacgcgcct
     2701 ccttggcgaa agcgatgagc tcgtcgattt gccgacgcat gccgacgttc ccgccgatgg
     2761 ccgagcgctc gaagtcgagc gtcgtcacgg ccatgtacca tccgcggttc tcctcgccga
     2821 tcatgttctt cttcggaacg cgaacgtcct cgaagaacac ctcgttgaag gagtggttgt
     2881 cggccatgtt gaccagcgga cgcacggtga ttccgggtga cttcatgtcg accatgaagt
     2941 acgagatgcc cttgtgcttc ggtgctgttg ggtcggtgcg tgcgagcaga atgccgtagt
     3001 cggcgatgtg cgcgcccgag gtccagatct tctggccgtt gacgacgtaa tcgtcgccgt
     3061 cacgcacggc gcgagtttgc agcgacgcga ggtcggagcc ggcgcccggc tcgctataga
     3121 gctggcacca ccagcgctcg cccgagagga tgccgcccag gtgttccttc ttctgctcct
     3181 cggtcccgta cacgatgagc gttggaccca ccatgccgac tccgatcatg tcattggggg
     3241 gagcctgtgc cttcgccacc tcctcgttga agatgaactg ctccatgatg ctcatggagg
     3301 cgccgccgta ctccttcggc caagcgggag cgatccatcc acgcgcggcc aacttcttgc
     3361 gccacgccgt gatgacctcg cgacggcctg tcccgaacat cgcttcctca tcgccgccgc
     3421 cgcgccaatc cggggcgagc tcggcgtcga gaaacgaccg tacctcgttg cgccatcgcg
     3481 cttgttccgg cgtgtctcgg aagtccatcg tgcttactcc ttcgtcgaag ttcgtcgcta
     3541 gccgcgaggc agaccgaggc cccgcatggc gataatgttg cgctgaatct cgctcgtgcc
     3601 gcccgcgata gtggccgata cggagcgaag atacatgcgc tcgaagcggc cccggagggg
     3661 cgcccaaggc gatccacgct ccaactgtcc gtaggtgccg atgagcttga gccccgtgcg
     3721 agcgatgcgc tgcgtcagct ccgtactgaa cagcttcgca cacgacgcct cgtggttggg
     3781 gatcatcccc tggctctgca tggtgatgat gcggtagctc atcagcatcg ccacggccac
     3841 ctcgatgcgg cggtccgcga gctcgtagcg aacgctcggc gtgtgtccta cggccatgaa
     3901 gggctcgttc cggttggcgt gcacgaagtt cagcaggtcc tcgaccatct gctccatgcc
     3961 gaccgcgttt ccgatggacg agcgctcgaa gtcgagcgtt gttgttgcca tgtaccagcc
     4021 gcggttctcc tcgcccagga ggtcgcgctt gggaatgcgg acgttgtcga agaagacttc
     4081 gttgaactcg tgcgtgccgg ccatgttgac gaggggccga atgctgatgc ctggcgtctt
     4141 catatcgagg aggaagtagc tgattccctt gtgcttgggg gcgtcggggt ccgttcgggc
     4201 cagaaggatg ccccgctgag ccctatgcgc gccggaggtc cagatcttct gtccgttgac
     4261 gacgtaatcg tcgccgtctc ggaccgcgcg cgtctgcagg gcggcgaggt cggagcccgc
     4321 gcccggctcg ctatagagct ggcaccacgt cacctcgccg gagaggattc cggacagatg
     4381 ctgctgcttc tgctcgtcgg tgccatagac gatgatggtg gggccggcca taccgacccc
     4441 gatgccacct ggacgcggcg cgcgggcgcg agccatctct tcgttgaaga cgaactgctc
     4501 cttgacgctg agtcccgcgc ccccgtattc cttcggccaa gcgggggcga tccagccccg
     4561 gctggcgagc ttcttcatcc actgctgata gcgttcctcg ccatccgctc gctgttcacg
     4621 ggggtcggtc tcgccttgcc agccgcccgg cagctcctgg cgcaggaatg cgcgcacctc
     4681 gtcgcgaaag gctgcttcct cggttgtatc gcgaaagtcc atgggctgcg tcctttcggc
     4741 atcccgcact cggaagcttg gcggcgaacc gaggcggcga cattggacgg aggatgggcg
     4801 gaaggaattg gcgcaccacg tgcgccagtg agcattatac gggcgacatg acgaaagtca
     4861 acgttcctgt tggtggctat cgaaagtgcc tatgggcgta aggttgtggc ttcgctggct
     4921 acgaaccagt agccctcgtt cgggcggagc tcggtgagcg tggcgagcga gccatcgggc
     4981 aggtagacga ggaacgctcc gtcgaggcca tcggtgaagc cgagaatcga cagcagagcg
     5041 ccctggatgg acgccacggt ctcggcgatg ggtaccgggc cgttgagcgg aaggcccacg
     5101 aggttgagac cggccacgag ctccaccgtc tcgggaggcg ggacgccgga atccggaacc
     5161 acgagggtca cgggccctga gacgcgcagc cacatcccgg cgagcggcgg aagttcagtg
     5221 aagtccgaca cgccggggtt gcccgggacg aagaagcgga agacttgctc cgcctcgtcg
     5281 aaggtgatcg cggagaccgc ggcatcgccg agcggcgcga gcagcgattc gatcgaggtc
     5341 gttgttggtg taaaggagaa ggcaacgagg ttccatcctg cctcgagctc gattcccgtg
     5401 cccgacgtgc ccagtgcggt ctcgaggtct atcgacgtga cggttccgtt ccagggcgct
     5461 tccactgccg tgttcgcccc ggccacggtg atgcgaactg tggcgccctc cgtgccgcat
     5521 ccgggctgca gcatcgccga ggcgacatcg agctgatatt cagaagagcc gtcgttcccg
     5581 aaggtcgaga ccgtcgatcc gcagagctgc tcgccgacgt acgcatcgat gcgaatgcca
     5641 ggaccggccg gagcgccctg cgaggtgacg cggcccagga aggttgctgg cacggcggcc
     5701 agcgcgacat tcgctcgcag gcggcccgcc ccatcccagt tgggctcgcc cgcgtccggg
     5761 aggtcctgag ccgccgcctt gatgatgtcc atgacctcga tcccggagag agtcgggttg
     5821 cgcgatagca tgagcgccgc agtcccggcg acgaacgggg ctgagaacga cgttccgtcg
     5881 cccgggtcgt agccgtcgtt gagcgagatg cgttcgtcgt cgcctccatc gagcgggcca
     5941 acgttgagcg gcgtgacgct ttcgacgctc aagtacgc
//
LOCUS       DATMBB010000085         3848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_65797, whole genome shotgun sequence.
ACCESSION   DATMBB010000085 DATMBB010000000
VERSION     DATMBB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65797"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VNL92_01805"
     CDS             <1..651
                     /locus_tag="VNL92_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93477.1"
                     /translation="GVDLDRFEPTSRAAARLALGLALDEPIVLFVGRIEPLKGIDLLI
                     DALSYVEPLGTGSPATLLIVGGDALAQREVEDLRHRAARTGVADRVRFVGSAPREMLP
                     LFYSAADVCVVPSHYESFGLVAVEAMACGTPVVASRVGGLTTTVQDGVTGYLVPWRCP
                     EPFAERIELLLANPYLRDSLGRAARRSVERLRWGHIAERVLALYRELAGAVAPGRA"
     gene            648..1367
                     /locus_tag="VNL92_01810"
     CDS             648..1367
                     /locus_tag="VNL92_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWO93478.1"
                     /translation="MMAGRPQGIQVDVVAFFAHPDDVDFLCGGTIAKLAAEGKRIWYV
                     LATSGDKGTSDRAIPPHELAVRREIEQRNAAMALGALGVSYLGYPDGFLEDTAEFRGK
                     LVRQLRLLRPDVVITFDPYRRSHNHRDHRTIGQATLDAAFPLARDHLAYPEHLTEEGL
                     EPHRVAEVWLAGPDDPDYYVDIGDHFHQRLRAIACHKSQVGDAGMSDEFEERMRQRSR
                     EVGEAAGYEFAEAFRRIQFRR"
     gene            1386..2264
                     /gene="ccsB"
                     /locus_tag="VNL92_01815"
     CDS             1386..2264
                     /gene="ccsB"
                     /locus_tag="VNL92_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HWO93479.1"
                     /translation="MEQLSIALFWVALGTTGLSAAVYLLYVFGQRMAVGRAATDAGNL
                     TVATAAVMPESLAWVGRLGAWTSTAVLTASLAARWIAAGRPPYSNMWEFTVAFAWGIS
                     VCYVFFEYRYRQRSLGAFVQPVTFALLLVATLFPSEISPLVPALQNDNILAYHVGAMI
                     LSYSAFSVAFGAAVTYLIQGKSRRFSRLPKADTLDAIAYRAVLVGFPLLALGLLLGAY
                     WGNSAWGRYWGWDPKETSALVTWLIFAGYLHVRNLSGWRGTRSAWLVIIGFVAVVFTF
                     YGVNLWVSGLHSYAGV"
     gene            2361..2627
                     /gene="rpsT"
                     /locus_tag="VNL92_01820"
     CDS             2361..2627
                     /gene="rpsT"
                     /locus_tag="VNL92_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWO93480.1"
                     /translation="MANTSSAKKRWRQNLRARERNKPIRTRARSAAKDAEQAIAKGEA
                     VDEALRVAASALDRAARKGVIHRNAAARKKSRLAHRARMANASA"
     gene            complement(2706..3116)
                     /locus_tag="VNL92_01825"
     CDS             complement(2706..3116)
                     /locus_tag="VNL92_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO93481.1"
                     /translation="MPVYEYYCESCNGIFEELRPMREASDPVPCPECFRDAKRIMPTS
                     FAAFTFRDGYPRRIPDKGTYYHLGKEVSKPVTGGVRPNEHPEINKPKPKPKLTKGERA
                     DRKERRMLERKQKERVARETPPPRPKKPASRTFV"
     gene            complement(3204..>3848)
                     /gene="recO"
                     /locus_tag="VNL92_01830"
     CDS             complement(3204..>3848)
                     /gene="recO"
                     /locus_tag="VNL92_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWO93482.1"
                     /translation="PKSKLGGHVEPLTHASLLLAQGRSLDIVSQAETIEPFASLRDDL
                     TRLTSGLYLAEMVDRFTEVGLAHDQDERVFVLLLGALRALDAGEHAAMVCRFLEMRLL
                     DAFGYRPQLQGCVVCSEPLVPSAQYFGASAGGAICPSCARSEGGPVRPLSLTALKVLR
                     LLQTKAFGEAARLHMAADVAREVEAHLRDSIHHALDRDVRSSAFLDHLRVSTAR"
BASE COUNT          621 a         1288 c         1241 g          698 t
ORIGIN      
        1 ggagtcgacc tcgaccgctt cgagccaacg agccgggcag ccgcccgcct cgctttggga
       61 ctcgcgctcg atgagccgat cgtcctgttc gtggggagga tcgagccgct gaaaggtatc
      121 gaccttctca tcgatgcact ctcctacgtc gagccacttg gaacgggcag tcccgcgacg
      181 ctcctcatcg tcggaggcga cgcgctcgct cagcgtgaag tcgaagacct gcgccaccgg
      241 gcggcgcgta ccggagtggc cgaccgggtt cgttttgttg gatcggcgcc gcgcgaaatg
      301 ctgcccttgt tctacagcgc cgccgatgtc tgcgttgtgc catcgcatta cgagagcttc
      361 ggactcgtcg ctgtggaggc gatggcctgc ggcacccccg tcgtcgcgtc gcgcgtcggt
      421 ggcctgacga cgacggtcca ggatggtgtc acgggctact tggttccgtg gcgatgtccg
      481 gaaccctttg ccgagcgcat cgagttgctg ctcgccaacc catacctccg cgattcgctt
      541 ggccgcgctg cgcggcgatc cgtcgagcgc ctgcgatggg gccacatcgc cgagcgcgtg
      601 ctcgccctgt acagggagct ggcgggagcg gtggcgccgg gccgagcatg atggcaggcc
      661 gcccccaagg tatccaggtc gacgtcgtcg cattctttgc gcatccggac gatgtcgact
      721 tcctatgcgg ggggacgatc gcgaagctcg cggccgaagg caaacgcatc tggtacgtcc
      781 tggccacgag tggcgacaag ggaacctccg atcgcgctat cccgccgcat gagttggcgg
      841 tgcggcgcga gatcgagcag cgcaacgcgg cgatggcact tggcgctctc ggcgtcagct
      901 atctcggcta tcccgacggc ttcctcgaag acacggccga gttccgcggc aagctcgtcc
      961 gccagcttcg cctgctgcgc cccgatgtcg tgatcacgtt cgatccgtac cgccgctccc
     1021 acaaccaccg cgaccatcgc actatcgggc aggcgacgct cgacgctgcg tttcccctgg
     1081 cgcgcgatca tctcgcatat ccggagcacc tcacggaaga gggactggag ccgcaccgag
     1141 tcgcggaggt ctggcttgcg gggccggacg acccggacta ctatgtggac atcggtgacc
     1201 attttcacca acgcctgcgg gcgatcgcgt gccacaagag ccaggtgggc gatgccggca
     1261 tgagcgatga gttcgaggaa cgcatgcgac agcgttcccg cgaagtcggc gaggcggccg
     1321 gctacgagtt tgccgaggcg tttcgccgca ttcaattccg ccggtagcgg cagaggcaat
     1381 tggtcatgga acagctttcc atcgcgctat tctgggtcgc actcgggacg accggactct
     1441 ccgccgccgt gtacctcctg tacgtcttcg gacagcgcat ggcggtcggc cgtgcggcca
     1501 cggacgcggg gaacctcacc gtcgccaccg ccgcggtcat gccggagagc ctcgcctggg
     1561 tcggacggct cggtgcatgg acatcgacgg ccgtcctgac cgcctcgttg gcggcacgtt
     1621 ggatcgccgc cggccggccg ccctactcga acatgtggga gtttactgtc gctttcgcct
     1681 ggggcatcag cgtctgctac gtcttcttcg agtaccgcta ccgccagcgc tcgctcggcg
     1741 cgtttgtcca accggtgaca ttcgcgcttc tgctcgtcgc aacgctgttc ccgtccgaga
     1801 tatcgcccct cgtgccggca ctccagaacg ataacatcct ggcctaccac gtgggcgcga
     1861 tgatcctgtc ctatagcgcg ttcagcgtgg cgttcggggc tgcggtgacc tacctcatac
     1921 aagggaagtc gcgccgattc tcgcgcctgc ccaaggcgga taccctcgat gcaatcgcgt
     1981 atcgggccgt cctcgtcggc ttccccctgt tggcgctcgg cctcctctta ggcgcgtact
     2041 ggggaaattc agcttggggc cgctactggg gatgggaccc taaggagact tccgccctcg
     2101 tgacatggct catcttcgcg gggtacctcc atgtgcggaa tctgagcggc tggcgaggca
     2161 ctcgttcggc ttggctcgtc atcatcgggt ttgtcgccgt cgtcttcacc ttctacggcg
     2221 tcaacctctg ggtatccgga ctgcactcct acgctggcgt gtagctcagc gcgcgcctca
     2281 atcttcgcga gcgcgagttt gctcggcgag cgctcgtctc gtatgctatg gagcgttact
     2341 agcacaacag aggagtatcc atggccaaca cctcgtcggc gaagaagcga tggcgccaga
     2401 acctgcgagc ccgtgagcgc aacaagccca ttcgtactcg cgcccgcagc gccgcgaagg
     2461 atgccgagca agccattgcc aaaggcgagg ccgtcgacga ggcactgcgt gtcgcggcca
     2521 gtgcactcga tcgggcggcg cgcaagggcg tgattcatcg caacgcggcg gcgcgcaaga
     2581 agtcgcggct ggctcaccgc gcccgaatgg cgaacgcgag cgcttaggct tgcaaccagg
     2641 gcacggacga cgataaggac gggactgctt tcgcagtccc gtcctgtgca ccctctcgtg
     2701 atcgtctaga caaacgtgcg gctggctggc ttcttcggac gaggtggcgg cgtctcgcgc
     2761 gccacccgct ctttctgttt cctctcgagc atccggcgct ctttgcgatc ggcccgctcg
     2821 cccttcgtca gcttgggctt cggcttcggc ttgttgatct cgggatgctc gttcggtcgc
     2881 acgccacccg tgaccggctt ggagacctcc ttgcccaggt ggtaatacgt ccccttgtcc
     2941 ggaatcctcc tcggatagcc atcgcggaaa gtgaaggccg cgaacgacgt gggcatgatg
     3001 cgtttggcgt cgcggaagca ctccggacac ggcaccggat ccgaagcctc gcgcatggga
     3061 cgcagctctt cgaagatgcc gttacacgat tcgcaatagt attcgtagac gggcatgctc
     3121 tccccctaag gacgttttcg gcaccctaac ataatggctc cgcccgagac aaggataccc
     3181 tcccccgccg ccctgcgggc cggctacctg gccgtcgaga cgcggagatg gtcaaggaac
     3241 gccgacgatc ggacgtcgcg gtccagggcg tggtggatgg agtcccgcag gtgcgcctcc
     3301 acctcccgag cgacgtcagc cgccatgtgc agccgcgctg cctcgccgaa cgccttcgtc
     3361 tgcaacaatc gcagcacctt gagcgccgtg agcgacagcg ggcgcacggg accgccctcg
     3421 gatcgggcgc aggatggaca gatggcgcca cccgcggacg cgccgaagta ctgcgcggac
     3481 gggacgagcg gctccgaaca caccacacag ccttgcaact gcggccggta gccgaatgca
     3541 tcgaggaggc gcatctccag gaaccggcag accatcgccg catgctcccc cgcatcgagc
     3601 gcccggaggg cgccgaggag gaggacgaac actcgctcgt cctggtcgtg cgcgaggcct
     3661 acctcggtga agcgatcgac catctcggcg agatacagcc cggacgtcag ccgcgtgagg
     3721 tcgtcgcgca acgaggcgaa gggctcgatc gtctccgcct gcgagacgat gtcgagactt
     3781 cgcccttggg cgagcagtaa cgatgcatgc gtgagcggct ccacgtgacc gccgagcttg
     3841 ctcttcgg
//
LOCUS       DATMBB010000086         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_146892, whole genome shotgun sequence.
ACCESSION   DATMBB010000086 DATMBB010000000
VERSION     DATMBB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146892"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VNL92_01835"
     CDS             complement(<1..1114)
                     /locus_tag="VNL92_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93483.1"
                     /translation="MPIRDLTASEVEWRSGPLAAAVVSDEPAFAFLAPTAADPAPPRV
                     SERIAEHAGWLARRGLDLIPFAIASVLITSMLWGTLFLPVPLAIGLLAFHAYWLYRSL
                     KIGLFGVIGYFRLRKHRRIDWRAKYDEARGKARAGADILAWEDIRHLIIIPTYLESIE
                     KLRPTLDGLAGQVEPDKLFIVLAMEERDLEAPEKAAILCEEYRDRFGGIFATLHPAGI
                     PGEVTGKSSNESWASVQAKRILIDQRGMSLDHFTVTSCDADCIFPPTYYACLTYKFAT
                     DPKRYRRFWQSPIFLYNNIWDVPAPLRVPSALGGLNHLSRLCQPFAVIFPQSCYSLSY
                     RLAVEVGHWDVDVVPEDWHMFLKCFFLTNGEVSVDRL"
     regulatory      complement(1256..1385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1718..2533
                     /locus_tag="VNL92_01840"
     CDS             1718..2533
                     /locus_tag="VNL92_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93484.1"
                     /translation="MQGLEMRINALRQAIAENDGENIAGPAFRREVEALVDAFYDDIG
                     HVTLVSARSLFDLIVIKTLYVDRASRDAAVVDYLGAMLTRHLYTRELFPIVRDNRRSL
                     MYISDLMEQMRNPVQPQNLFEHYRQFGDNALFITGVLPNSVRPGRRRPRGFVDQAYYI
                     TSGKAFYRRAGEHELAWLTQQRETLLKLSRHFEVYMDALNDASENFILGLDMNVIANK
                     MLDAFNRYRASGEEGDLENARKYAALLKVDAKNFPALFRRRPGRAVLLDGPRA"
     gene            2604..2882
                     /locus_tag="VNL92_01845"
     CDS             2604..2882
                     /locus_tag="VNL92_01845"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWO93485.1"
                     /translation="MAKLNDDEIARQASRVPEWTREGDAIRREFRFKDFVRSMGFVNQ
                     VALLAEKADHHPDIAVSYNRVGLELSTHSEGGLTARDFDLAAQIDALI"
     gene            2940..3215
                     /locus_tag="VNL92_01850"
     CDS             2940..3215
                     /locus_tag="VNL92_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO93486.1"
                     /translation="MPIYEYECPNCRHRDQLYVRSVNNQPSPPACPQCGQEAMRRLIS
                     RALYIQDEATKVEQLDPKYEKMIDDADKPFKANDPLKRITGTPLYPD"
BASE COUNT          655 a         1026 c          989 g          556 t
ORIGIN      
        1 agagccggtc gacgctcacc tcgccgttcg tgaggaaaaa gcacttcagg aacatgtgcc
       61 agtcctccgg caccacgtcc acatcccagt gaccaacctc gacggccaag cgatagctca
      121 agctgtaaca cgactgcgga aagatcacgg cgaatggctg gcacagcctc gacaggtgat
      181 tcagaccgcc gagagcgctc ggcacgcgaa gcggcgccgg tacatcccag atgttgttgt
      241 acaggaagat gggcgactgc cagaacctgc ggtagcgctt tggatccgtg gcgaacttgt
      301 aggtaaggca cgcgtagtac gtgggcggga agatgcaatc ggcgtcacag ctggtgacgg
      361 tgaagtgatc gaggctcatc ccccgctggt cgatgaggat gcgcttggcc tgcacgctcg
      421 cccaggactc gttcgacgac ttgcccgtaa cctcgcccgg aatcccggcg gggtgcagcg
      481 ttgcgaagat gccgccaaag cggtctcggt attcctcgca caggatggcc gctttttcgg
      541 gcgcctccag gtcacgctcc tccatcgcca gcacgatgaa caacttgtcg ggctcgacct
      601 gtcccgcgag accgtcgagc gtcggccgca gcttctcgat cgactcgagg tacgtgggaa
      661 tgatgatgag atggcgaata tcctcccatg cgaggatatc ggccccggcg cgcgccttcc
      721 ctctcgcctc gtcgtacttc gccctccagt cgatcctgcg gtgctttcgc aaacggaagt
      781 agccgatgac accgaagagg ccgatcttga gcgaccggta gagccagtac gcgtggaacg
      841 ccagcagccc gatggcgagc ggcacaggca aaaagagtgt gccccaaagc atcgaggtga
      901 tgagcacgga cgcgatggcg aacgggatga gatccagtcc tcgccgagcg agccatcctg
      961 catgctccgc gattcgttcg ctgacgcgtg gcggcgctgg atcggccgcg gtgggggcca
     1021 aaaaggcgaa cgcgggctca tccgatacga cggctgccgc gagcgggccc gaccgccatt
     1081 cgacttcgga ggcagtcagg tcgcggatgg gcaaacggtg gggcgaaagt acggggtcgc
     1141 gggcgagtcg gtccagcagg ggacacctcc agcgctatga caggaggacc tctgggaacc
     1201 gaatcttcgg aaccacgagc tccgaacgta gggagggttc ccaggagtat cctgacgaac
     1261 cttccaacgc ctacggggtt agctgacggg tgagggccga aaggtgcccc tccctgccat
     1321 gtcgcaggga ttaaccccag ggtttggttc ccccgccttc atgcgaagtt cggcgactac
     1381 ccgagccgtg cttgctcgat gaggctaggc catgggcatg ctccatgtca atccttcaac
     1441 ggcgcctatg tcgaaggctc gcctctccgg cgccgtgtcc gtcctttccc ttcgcactca
     1501 tctgtcttac gattcggtta cactccactt catctcgcag gggagtgcga gcttacgatc
     1561 cgatgaaaca ctcccgaaac tatgggggac aatactcagt acggatgttg gctcacggcc
     1621 agcaccactg tcgaaggcgg cggagttcga ccgaaaggcc gcacgccgat gccggagacg
     1681 ccccgccgaa tggcggagac caaccgggag gtaactgatg caaggactgg agatgcgcat
     1741 caatgccttg cggcaagcca ttgccgaaaa cgacggcgag aacatcgctg ggcccgcgtt
     1801 tcgtcgcgag gtcgaggcgc tcgtggatgc attctacgat gacatcggac acgtcaccct
     1861 cgtgtccgcc cgaagcctct tcgacctcat cgtcatcaag acgctctatg tcgaccgggc
     1921 gagccgcgac gccgccgtcg tcgactacct gggcgccatg ctgacccgcc acctctacac
     1981 acgagagctg ttcccaatcg tgcgggacaa ccgccgctcg ctgatgtaca tctccgacct
     2041 catggagcag atgcgcaacc ccgttcagcc ccagaacctg ttcgaacact accggcagtt
     2101 cggcgacaac gccctcttca tcaccggcgt gttgccaaac agcgtgcgcc cgggccgccg
     2161 ccggccgcga ggtttcgtcg atcaggccta ctacattacg tctggtaagg ccttctatcg
     2221 gagggcaggg gagcacgagc tggcgtggct cacgcaacag cgcgagactc tgctcaagct
     2281 ctcccggcat ttcgaggtgt acatggatgc gctgaacgac gccagtgaga acttcatcct
     2341 ggggctcgac atgaacgtca tcgccaacaa gatgctcgat gcgttcaacc gttaccgagc
     2401 gagcggcgag gagggcgatc tcgagaacgc gagaaagtac gccgccctcc tcaaggtcga
     2461 tgccaagaac ttcccggcgc tcttccggcg ccgaccgggc cgcgccgttc ttctcgacgg
     2521 accgagagcg tagctcccga agctgcgcca ttgccgccgg gtgctacact cacgtcgaag
     2581 tcggcggtcg aggaggtagg agcatggcaa agctgaacga tgacgagatc gcgcgccagg
     2641 catcgcgcgt accggagtgg acccgggagg gcgatgcgat ccgccgcgag ttccgtttca
     2701 aggacttcgt tcgcagcatg ggctttgtga accaggtggc cctgctcgcc gagaaggcgg
     2761 accatcatcc cgatatcgcc gtcagctaca accgcgtggg cctcgagctc tcgacccaca
     2821 gcgagggcgg cctcactgct cgggacttcg accttgccgc gcagatcgat gccctgatct
     2881 gacgcctgga ttcgattggt gcggtatgat agagccgtag cgcaagcgag gatcatgcca
     2941 tgcccattta cgaatacgag tgccccaact gtcgccaccg cgaccagctc tatgtccgca
     3001 gcgtcaacaa tcagccatcg cctccggctt gtccgcaatg tggtcaggag gcgatgcggc
     3061 gtctcataag ccgcgccttg tacattcaag acgaggcgac gaaggtcgag cagctcgacc
     3121 ccaagtacga gaagatgatc gacgacgcgg acaagccctt caaagcgaac gacccgctca
     3181 aacgcatcac cggcacgcca ctttaccccg actagcgctc cgggga
//
LOCUS       DATMBB010000087         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_543007, whole genome shotgun sequence.
ACCESSION   DATMBB010000087 DATMBB010000000
VERSION     DATMBB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543007"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VNL92_01855"
     CDS             complement(<1..256)
                     /locus_tag="VNL92_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014016905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWO93487.1"
                     /translation="MNGGPRVLVAMSGGVDSAVVAGLMKQRGYEVIGVTMRLWAPEDD
                     RLPRGNRHCCSLDDIDDARAAAHQLGIPHYVVNMEDVFQER"
     gene            complement(355..498)
                     /locus_tag="VNL92_01860"
     CDS             complement(355..498)
                     /locus_tag="VNL92_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93488.1"
                     /translation="MPCGRGRGDDEVVRKRGDSTDIEQDDVLGLAVREPVDDLTCYLS
                     ALQ"
     gene            436..702
                     /gene="rsfS"
                     /locus_tag="VNL92_01865"
     CDS             436..702
                     /gene="rsfS"
                     /locus_tag="VNL92_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWO93489.1"
                     /translation="MSAIASFADYFVIATATSARQMDALIDGLRQALGAKGVSPARQE
                     GTPDSGWVLIDYGDVVVHLFSQESRDYYRLEQVWSGGVPLVRIQ"
     gene            complement(706..1155)
                     /gene="ndk"
                     /locus_tag="VNL92_01870"
     CDS             complement(706..1155)
                     /gene="ndk"
                     /locus_tag="VNL92_01870"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWO93490.1"
                     /translation="MERTLILIKPDAMQRGLAGEILARFERRGLRIVGLKLMQVDEDL
                     ARRHYAEHEGKPFFEALVRYICSSPIIAGVLEGTSAIEAVGQAVGATNPVKAAPGTIR
                     ADFGLEIGRNLVHRSDSMESAQREIALFFGEEPLPSWERAIDRWVFE"
     gene            complement(1184..1792)
                     /locus_tag="VNL92_01875"
     CDS             complement(1184..1792)
                     /locus_tag="VNL92_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93491.1"
                     /translation="MGAEARCVAYVDGAAVEGKALLETSELLFRGEGVRLAIPFSDMT
                     AVEAVDGHLRIAYAGGEAALDLGDTAAKWARKILNPPSLMDKLGVKPGMRVSMVGRIE
                     GDLARQVRERGALLSENGSAEADLVFLAVDTREDLAQLDRLRRRLAPKGAIWIIRPKG
                     SKQISEAEAMAAGKAAGLVDVKVARYSDTHTAEKFVIPVSSR"
     gene            complement(1793..>2260)
                     /gene="tsaB"
                     /locus_tag="VNL92_01880"
     CDS             complement(1793..>2260)
                     /gene="tsaB"
                     /locus_tag="VNL92_01880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWO93492.1"
                     /translation="YMGVRVAMATAKGLALALGLPIVGVNRLSLDAWPHRAYAGPIVA
                     VHKAGRRQWAWAAYAWDPPAWRELQAPRLDDAESLADAAPAGLLLCGEPDEELCAAVR
                     PPRVVRGESAARRPAGLCELGWLALQERGADDLAGLAPIYLREPAIGPQQEVG"
BASE COUNT          393 a          824 c          674 g          369 t
ORIGIN      
        1 cccgctcctg gaagacatcc tccatattga cgacgtagtg cggaatgccg agctgatgcg
       61 ccgccgctcg cgcgtcgtcg atatcatcca atgagcagca atgccggtta ccgcgcggca
      121 gccggtcatc ttccggcgcc cagagtcgca tcgtaacgcc gatgacctcg tagccgcgct
      181 gcttcatcag cccggcaacg acggccgaat cgacgcctcc gctcatagca acgaggacgc
      241 gggggcctcc gttcacgtct gcaacctcga cctggccttc ttcctgagat tcgcctacgt
      301 caatggtata ctggcgcaac acgtccgcac cagactcgcc acaagggagc agcgtcactg
      361 gagagcgctg agatagcacg tgagatcgtc gaccggctct cggaccgcca ggccgaggac
      421 atcgtcctgc tcgatatcag tgctatcgcc tcgtttgcgg actacttcgt catcgccacg
      481 gccacgtccg cacggcaaat ggacgctctc atcgatgggc tgcgtcaggc cctcggcgcg
      541 aagggcgttt cgcccgcgcg ccaggaaggg acacccgact caggctgggt actcatcgac
      601 tacggcgacg tcgtcgtgca cctgttctcg caggagtcga gagactacta ccgcctcgag
      661 caggtctgga gcggaggcgt gcccttggtg cgcatccagt agcgcctact cgaagaccca
      721 gcggtcgata gctcgctccc acgaggggag cggttcctcg ccgaagaaga gcgcaatctc
      781 cctttgggcg ctctccatcg agtcggaccg gtggacgagg tttcggccga tctcgaggcc
      841 gaagtccgcc cgaatcgtgc cgggagcagc cttcaccggg ttcgtcgccc cgacggcctg
      901 ccccaccgcc tcgatggcgc tcgtcccctc cagcactccc gcaatgatgg gcgaggagca
      961 gatgtaccgg accagcgcct cgaagaaggg ctttccctcg tgctccgcgt agtgccttcg
     1021 ggccaggtcc tcatcgacct gcatcagctt gaggccgacg atccgcagac cccgccgctc
     1081 aaaacgcgcg agaatctcgc cggccaggcc ccgctgcatg gcatccggct tgatgagaat
     1141 gagtgtacgt tccaacgcct ctccattcgt cacgtcggtc gcgttagcgg ctcgacacgg
     1201 gaatcacgaa cttctccgcg gtgtgcgtgt cggaataccg cgccaccttg acgtcgacca
     1261 gacccgccgc ctttcccgcg gccatcgcct cagcctcgct gatctgcttc gaacccttgg
     1321 gccggatgat ccagatggct cccttgggcg ccagacgacg cctcaggcga tcgagctggg
     1381 cgaggtcctc gcgtgtgtcg acggccaaga agacgaggtc ggcctcggca ctcccgttct
     1441 cgctcagcag ggcgccccgc tcccgtacct gacgagcgag gtcgccctca atacgaccca
     1501 ccatggacac gcgcatgccc ggcttcaccc ccaacttgtc catcagcgag gggggattca
     1561 gaatcttccg cgcccacttc gcggcggtgt cgcccaaatc gagcgccgcc tcgccacccg
     1621 cgtacgcgat tcgtaagtgg ccgtccacgg cctctaccgc cgtcatgtcg ctgaacggga
     1681 tagctagacg aacgccctcg ccgcggaaga gaagctcact ggtctccagg agcgccttcc
     1741 cctcgaccgc tgcgccatcg acatatgcaa cacaccgggc ctctgcgccc atctacccca
     1801 cctcctgctg cggcccaatg gccggctcgc gcaagtatat cggagcaagg cccgcgaggt
     1861 catcggcgcc tcgctcctgc agcgccagcc agccgagctc gcagaggccc gcaggacggc
     1921 gagccgccga ctccccgcgc actacccgag gaggccgaac cgccgcgcac agctcttcgt
     1981 cgggttcgcc acacagcagc aagccagccg gagccgcgtc cgcaagcgat tccgcatcgt
     2041 cgagccgtgg ggcttgcagc tctcgccagg cgggcgggtc ccaggcgtac gcggcccagg
     2101 cccactggcg ccgcccggcc ttgtgcacag cgacaatcgg accggcatac gctcgatggg
     2161 gccaggcatc gagcgagagc cggttgacgc cgacgatcgg tagcccgagc gccagcgcga
     2221 gtcctttcgc cgtggccatc gccacacgta cgcccatata
//
LOCUS       DATMBB010000088         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_582883, whole genome shotgun sequence.
ACCESSION   DATMBB010000088 DATMBB010000000
VERSION     DATMBB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582883"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNL92_01885"
     CDS             complement(<1..222)
                     /locus_tag="VNL92_01885"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWO93493.1"
                     /translation="MFPSSPVFRLLGLGWFVAISILLGTVGGVWLDDQLSTAPLFTLV
                     GIFLGLGAAFVGAYQMLAETVLRPTGRRGG"
     gene            complement(342..893)
                     /locus_tag="VNL92_01890"
     CDS             complement(342..893)
                     /locus_tag="VNL92_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HWO93494.1"
                     /translation="MIELAIVSIRVSLMNYQRVVILKEKDADRYLPIWIGPAEADAIA
                     VRLQDVAVARPLTHDLLRSVIETLGGQVTSIVVNDLANDTFFARINLDVNGTPIEVDS
                     RPSDAIALAVRSQVPIFVAEQVMEKAGVLLEEDDEAGDGTGAARPAFEESAPVKEEDL
                     ERMSAFRDFIESLDLDDFDRRRS"
     gene            complement(949..2412)
                     /locus_tag="VNL92_01895"
     CDS             complement(949..2412)
                     /locus_tag="VNL92_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93495.1"
                     /translation="MTTTTRPPTERELPPHEDVGPAELMWNRDFALLWLMHAVSQTAH
                     NAVLFSLLVIVTTDTGSSIYASLLVLSFISPAVFVGLFAGVLVDRSRKRTVLATTSAI
                     RAAICLAFPFVTESVWAIYGLTIAFSIVAQLFNPAVVTVIPKLVPRSHLLAANGLFNV
                     TLTGGQFAGMVVLSPLMLKAFGDEAMFGTAAVLLLGSAIVALVMPHLDEPPRDEDAAT
                     YGLRDVVADVRTSLRRLRSDQEASRAMSQLVLGSSLILLVVVLIPIYMGAVLDVGAEN
                     AAFVFAPAGLGAALGLRAIPRAATRYPHATRVAMGFGAVALAFLAISLVEPVGALLGQ
                     TTEYDPFSGDRQPGLGVMVGLTMVFSIPFGFGYAMANASAQTVLHDRAPEEMRGRYFA
                     TQIALANAISIPALLLLGAAADIVGVSPVIAILGAGILAASYVSGRPSGFSLPRGRQA
                     NPRNGRREDLPEDLPPAELDDDERSEVGSHSDLTRSR"
     gene            complement(2409..>2641)
                     /locus_tag="VNL92_01900"
     CDS             complement(2409..>2641)
                     /locus_tag="VNL92_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93496.1"
                     /translation="ELQGRVFAMQGVLSSLGALAPLLLGGALAELLGVRAVIIGIGVV
                     AAAVGLYTRLGERVNARRSREEGGAGFEEAYR"
BASE COUNT          527 a          888 c          840 g          386 t
ORIGIN      
        1 gccgcctcgc cggcccgttg gcctgagcac cgtctccgcg agcatttggt atgcaccaac
       61 gaacgcggcg ccgaggccca ggaagattcc gacgagggtg aataaagggg cggtcgatag
      121 ctgatcgtcc agccagacac cgccaaccgt tccgagcaag atactgatgg cgacgaacca
      181 gcctagaccg aggagccgga acaccggcga cgacgggaac atgcggcatt ccccggaact
      241 tgggctggcg tccggacttt gcgcactata tcacgcacga tttcgagggg tcaacacact
      301 ccgcgtggga ataacggcga tgccgcgatg tgcccgcact cctatgagcg ccggcgatcg
      361 aaatcgtcga ggtcgaggct ctcgatgaag tcccggaagg cggacatcct ctcgaggtcc
      421 tcttccttca ccggagcgga ctcctcgaac gcagggcgtg ccgcccctgt gccgtctccc
      481 gcctcgtcgt cctcctcgag caggacgccg gccttctcca tcacctgctc cgcaacgaag
      541 atagggacct gggagcgcac cgcgagggcg atggcgtcgc tcggacgcga gtcgacctct
      601 atcggcgtcc catttacgtc gaggttgatg cgagcgaaga acgtatcgtt cgcgaggtcg
      661 ttcacgacga tcgaggtcac ctggccaccc agcgtctcga tgaccgatcg cagaaggtcg
      721 tgggtcaacg ggcgcgccac ggccacgtcc tgcaatcgca cggcgattgc gtcggcttcc
      781 gccggcccga tccagatcgg caggtaccga tcggcgtcct tttccttcag gatgacgacg
      841 cgctggtagt tcatgagcga cacacggatg ctgacaatgg ccagctcgat cacgtcgtca
      901 cctcctgcat gcgcgatacc ctcattctag aagcgctcgg aagcgcagtc aacgagagcg
      961 agtcaggtcg gaatgcgatc cgacttcgct ccgctcatcg tcgtccaact cggccggcgg
     1021 aaggtcctca ggtaggtctt cccggcgccc gttcctggga ttggcctgcc ggcctcgagg
     1081 taaagagaaa ccagatggcc gtccactgac gtacgaggca gccagaatac cagcaccaag
     1141 tatcgcgatg accggcgata cgccgacgat gtctgcagcg gccccgagga ggagcaacgc
     1201 cgggatggag atggcgttcg ccagggctat ctgcgtggcg aagtagcgac cacgcatctc
     1261 ctcgggggcc cggtcgtgca gcacggtttg cgcggacgcg ttcgccatcg catagccgaa
     1321 gccgaagggt atggaaaaca ccatcgtgag cccgaccatc acgcccaggc ccggctgccg
     1381 atcaccggaa aacggatcgt attcggtggt ttggccaagg agcgcgccga ctggctcgac
     1441 gagcgatatg gccagaaagg ccaaggcgac ggcgccaaag cccatcgcga cgcgtgttgc
     1501 gtgcgggtac cgggtcgccg cacgggggat ggcccgtaga cccagcgcgg cgcccaagcc
     1561 cgccggggcg aacacgaaag ccgcgttctc ggcccccaca tcgagcactg cccccatata
     1621 gatcgggatg aggacgacga ccagcaggat cagggagctg ccgagcacga gctgactcat
     1681 cgctcggctc gcctcttggt ccgagcgcag ccgccgaagg ctcgtccgca cgtccgccac
     1741 cacgtcccga aggccgtacg tcgcggcgtc ctcatcgcgc ggaggctcgt cgagatgcgg
     1801 catgaccaag gcaacgatcg cgcttccgag caggagcacg gccgcagtgc cgaacatcgc
     1861 ctcatcgcca aatgccttca gcatcaacgg cgagagcacg accatcccgg cgaattggcc
     1921 gcccgtcagc gtcacgttga acagcccgtt cgccgccaac aagtgcgagc gcggcaccag
     1981 cttgggaatc acggtcacca ccgccgggtt gaagagctgc gccacaatcg agaacgcgat
     2041 cgtcaggccg tagatggccc atacgctctc ggtcacgaac gggaacgcca ggcatatcgc
     2101 ggcccgaatc gcgctcgtcg tggcgagaac ggtgcgcttc cgcgaccgat cgaccaggac
     2161 ccccgcgaac aacccgacga agaccgcggg ggagatgaac gacaacacga ggaggcttgc
     2221 gtagatcgag ctccccgtgt ccgtcgtcac gatgaccagg aggctgaaga ggacggcgtt
     2281 gtgcgccgtc tgcgagacgg catgcatcag ccagagcaag gcaaaatctc ggttccacat
     2341 cagctccgcc gggccgacgt cctcatgcgg aggcagctct cgctcggtgg gagggcgagt
     2401 ggttgtcgtc accgatacgc ctcctcgaac ccggcgccgc cctcctcgcg cgaacgccgc
     2461 gcgttcacgc gctcgccgag acgcgtgtag aggcccacag cagcggcaac aacgccaatc
     2521 ccaatgatca cggcccggac tccgagaagc tcggccaagg cgcccccaag cagcaggggc
     2581 gcgagcgcgc cgagcgagga cagcacgccc tgcatggcga agacgcggcc ctgcagctcc
     2641 g
//
LOCUS       DATMBB010000089         2761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_463662, whole genome shotgun sequence.
ACCESSION   DATMBB010000089 DATMBB010000000
VERSION     DATMBB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463662"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VNL92_01905"
     CDS             <1..923
                     /locus_tag="VNL92_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93497.1"
                     /translation="IPDRWPYESLLPRGAVPPMASTPSLGYTIFDKAEVWADSAGHLY
                     EEGIQRRWVPALDVDWTSLQELPSEVEHAMCQICTDLGEQALTKARVLGRWLPEISYG
                     YHESKVFLGQEIFDLDRIFEALRKRSLANGGGLGVQAGGLFVKAIFDSPEWVVVNFLT
                     HVLGASFAITEFAMLEAAAPHAAEKKLFRYVMQDLGRHARYGGESMRLLLHKQPHRKG
                     EVTAYIGLGEAQMAAEIRANKALREAYIILFGHKAGSLAKGKAMLDHMRRLQVESYLR
                     RLDWIGLKNWDTTGLNPALREFIEPAAALA"
     gene            complement(1015..>2761)
                     /gene="recJ"
                     /locus_tag="VNL92_01910"
     CDS             complement(1015..>2761)
                     /gene="recJ"
                     /locus_tag="VNL92_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWO93498.1"
                     /translation="NAVSQPVPPGFAKVQRGGRYRWRIRAVDKAAAVLGNGAYPPLVR
                     HLLWGRGVRTDLEALRFLDEATGPSVAVTHDPFLLPDMDRAVARLKSARAQGELVAAW
                     GDFDVDGVTSCALLLEGLGGLGFHVIAYIPDRFTEGYGLNQEGIAALRRKGVSVIVTA
                     DCGTSNVAEVAYARELGMDVIVVDHHTTPPLLPDAHAIVNPKVPDSPYPCAELAACGV
                     VFKLLQALYDACGRQFVEEDYIDLVALGTVVDMAPLQDENRDIVRRGLRELRRSRRPG
                     VRALMDVAGVAPEGVSARDLGFGLGPRLNAAGRLAHARQALELLLAQDEGHAHELAME
                     LNALNLERRRQTEEAVTLARQIVTARDELAPLVFVGDRSFPSGVVGLVASRLVEELHR
                     PAIVFQEGELESRASARSIPVFDIVSALRPHADLMVRFGGHRQAAGFTVENVHREALR
                     AALERGASELLSAADLEPTIDIDAAVPLGRLRGEDIRYIGKFAPFGIGNPEITLLSRG
                     AEVLECRPVGGDGEHLRLKLRDQGITWPAIAFRQAASGLEPGTRADVVYSLTPDIGPA
                     GLLQLTVRDFALSSA"
BASE COUNT          486 a          932 c          909 g          434 t
ORIGIN      
        1 atatccccga ccgctggccc tacgaaagcc tgcttccgcg tggggccgtg ccgcccatgg
       61 cttcgacccc ctcgcttggc tacaccatct ttgacaaggc cgaggtgtgg gccgactcag
      121 caggccacct ctacgaggaa ggcatccagc gacggtgggt gcccgctctc gatgtcgatt
      181 ggacatcgct ccaagagctg cccagcgaag tcgaacatgc catgtgccaa atctgcaccg
      241 atctcggcga gcaggcgtta acgaaggccc gagtgctcgg ccgctggctc cccgagatca
      301 gctatggcta ccacgaatcg aaggtcttcc taggacagga gatcttcgac ctcgaccgca
      361 tattcgaggc cctgcgaaag cgctcgttgg ccaatggcgg cggtctaggt gtgcaagcgg
      421 gcggcctgtt cgtgaaggcc atcttcgatt cgccggagtg ggtcgtcgtg aacttcctca
      481 cccacgtgct cggcgcatcg ttcgccatca ccgagttcgc catgctggag gccgctgccc
      541 cacatgcggc cgaaaagaag ctcttccgct acgtgatgca ggacctcggg cgccacgcaa
      601 ggtatggcgg cgagtcgatg cggctgttgt tgcacaagca acctcaccgg aagggcgagg
      661 tcacggccta catcggactg ggcgaagcgc agatggcggc cgagatccgc gccaacaagg
      721 cgctgcggga agcctacatc atcctcttcg gccacaaggc cgggagcctc gcgaagggca
      781 aggccatgct cgaccatatg cgacgcctgc aggtggagag ctacctacgc cggctggact
      841 ggataggcct gaagaattgg gacaccaccg ggctcaaccc agccctgcgg gagttcatcg
      901 agcctgcggc cgctctagcg taacgagcga cacactaccc gtggtatggg ggaggccggt
      961 tggcctcccc catcgttgtt cttctcgcag cgtagccccg tttgccgtta ggtatcacgc
     1021 cgaggagagg gcgaagtccc ggaccgtcag ttgcagcaag ccggccgggc cgatatccgg
     1081 cgtcaaggaa tacacaacat cggcgcgcgt tccgggctca aggcctgagg ccgcctggcg
     1141 gaaggcgatc gccggccagg tgatgccttg gtcgcggagc ttcagccgga ggtgttcccc
     1201 atcgccaccg acgggacggc actccagaac ctccgcgccc cggctcaata gcgtgatctc
     1261 tggattgccg atgccgaagg gtgcgaactt gccgatgtag cggatgtctt cgccgcggag
     1321 acggccgagc ggcaccgctg catcgatatc gatggtcggc tccagatcag ccgccgaaag
     1381 cagttcgctg gcgcctcgct ccagcgccgc gcgcagcgcc tctcgatgga cgttctccac
     1441 cgtgaatccc gccgcctgcc ggtgcccccc gaagcgaacc atgaggtcgg catggggccg
     1501 caaggccgac acgatgtcga acaccgggat gctccgcgcg ctggccctgc tctcgagctc
     1561 gccttcctgg aacacgatcg ctggccggtg gagctcctcg acgagcctgc tggcgacgag
     1621 gccgacaacg ccggagggaa agctccggtc tccgacaaac accagcggcg cgagctcgtc
     1681 ccgtgcggtg acgatctggc gggccagcgt cacggcctcc tccgtctgac ggcggcgctc
     1741 gaggttgagc gcgttcagct ccatcgcgag ttcgtgcgcg tgcccctcgt cctgcgcgag
     1801 aagaagctcg agcgcctgcc gtgcatgggc gagacgacca gcggcgttca gacgcgggcc
     1861 caaaccaaag ccaaggtcgc gggcgctcac gccctcgggg gccacaccag cgacgtccat
     1921 gagggcgcgc actcccgggc ggcggctgcg gcggagctcc cgcaagcctc gacgcacgat
     1981 atctcggttc tcatcctgca gcggcgccat atcgaccacc gtgccgaggg cgacgagatc
     2041 gatgtagtcc tcctcgacga attgccgccc gcacgcgtcg tacagcgcct gcagtagctt
     2101 gaagacgact ccgcaggccg cgagctcagc gcacgggtat ggcgaatcag gtaccttcgg
     2161 gttcacgatc gcatgggcgt cgggcagcag gggcggggtc gtgtggtgat cgaccacgat
     2221 gacgtccatg cccaactcgc gggcgtacgc cacttcggcg acgttgctcg tgccgcagtc
     2281 ggccgtgacg atcaccgaaa cgcccttgcg ccgtagggca gcgatgccct cctggttcaa
     2341 cccgtagccc tcggtgaacc gatccgggat ataggcgatg acgtggaacc ccaggccgcc
     2401 gagcccctcg aggagcaggg cgcatgaggt gacgccatcg acgtcaaagt cgccccaggc
     2461 cgccaccaac tccccctggg ctcgcgccga cttgagccgc gcaacggcgc gatccatatc
     2521 cggcaggagg aacggatcgt gcgtcactgc cacgctcggg ccagtcgcct cgtcgaggaa
     2581 gcgaagcgcc tcgaggtcgg tccggacgcc gcgaccccac aggaggtgcc gcacgagcgg
     2641 ggggtacgca ccgttgccga ggacggcggc ggccttatcg acggccctga tacgccaccg
     2701 gtagcggccg ccgcgttgga ctttggcgaa gcctggcggg accggctgtg atacggcgtt
     2761 g
//
LOCUS       DATMBB010000090         4145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_582775, whole genome shotgun sequence.
ACCESSION   DATMBB010000090 DATMBB010000000
VERSION     DATMBB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582775"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VNL92_01915"
     rRNA            complement(<1..352)
                     /locus_tag="VNL92_01915"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(734..2170)
                     /gene="tilS"
                     /locus_tag="VNL92_01920"
     CDS             complement(734..2170)
                     /gene="tilS"
                     /locus_tag="VNL92_01920"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWO93499.1"
                     /translation="MPMPEAPRESLTGDYRNLRLVRAFEHRVLAALRTECLVRPGDRV
                     LVGVSGGPDSSALLLALVAARAELPLDLTAVHIDHGVRPPEETDADLRFVRFLAERAG
                     VPLLTERAFPASSSEEALRQARYAALLTAARRSGALIVALGHTMDDQAETVLMRLLRG
                     AGLQGLGAMAPRAPWPFPASPPGPDIVRPLLSVRRAETEAYMQAHCLQPRRDPTNDDR
                     RYLRNRVRQDLVPLLERENPRAVAAMANAASSIREAAQLIETEKERAWPEATEEREGA
                     LFLRAEVLAALPAAVVTAVLAQALVWAGLGADAAALERLRSLLTGRAGRSATLPAGVR
                     AVRLAHDVRIEVAGGASASGPLPETRLAVPGETVVGPWIVRAAAWDGVAGDDSAVIVP
                     VSVAERGLWARSRRPGDRLRTARGSRKVQDILVDAKVPRWERDRVPLIVDAEGHILAV
                     SGLAATPFPSGTPAGSVRIAFEPSEMRA"
     gene            complement(2178..2513)
                     /locus_tag="VNL92_01925"
     CDS             complement(2178..2513)
                     /locus_tag="VNL92_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWO93500.1"
                     /translation="MPAITPTRIVFAVIALVVIYFGATAVSNATDHYSLDREREALQR
                     EIAEYQVQYDQLQGIRDYLATDEYIETTARRELGLVMPGEPSVIVISPPRESAEDADG
                     TWWQRSFIH"
     gene            complement(2637..3311)
                     /locus_tag="VNL92_01930"
     CDS             complement(2637..3311)
                     /locus_tag="VNL92_01930"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWO93501.1"
                     /translation="MRLILVRHAETDHNRDGFAQRRADNPLNETGRAQCVAVAQALAH
                     ESVVAVRSSPLRRAMETAEAIAGRHGLAVEPEPDLIEMDVGVMDGLSTREMREQHAEF
                     LRAWMGPGAATTPMPGGESLAQVQDRAWRTVERLMDEHASGVVVLVSHNFVIATLICR
                     ALDIDLADFRRIRQDVAACHALDLTPERTRVVRLNETCHLYGGRLPPMAAFWRRGVVD
                     ARQGDA"
     gene            complement(3334..3927)
                     /locus_tag="VNL92_01935"
     CDS             complement(3334..3927)
                     /locus_tag="VNL92_01935"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWO93502.1"
                     /translation="MSMKHALLGFLSYKPMTGYELKQAFDSTVHFFWNAELSQIYPAL
                     KQMERDGLLTMHIEPQSDRPNRKVYELTDAGHAALIEWMQEPTDLPIMKDAFLIKVFF
                     GAALQPELMRQHLNEQLDAHRERLRYYETSNWESLRDIVARNPTMPDPNYWVLTLEWA
                     LRYERTCIEWCEWALDRLDRIGEDATRALEMANSRSS"
BASE COUNT          695 a         1360 c         1374 g          716 t
ORIGIN      
        1 ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggctg gccgtcctct
       61 cagaccagct accgatcgtc gccttggtgg gccgttaccc caccaactag ctaatcggcc
      121 gcaagcccct ccgaaagcgc ctttcggctt tcctcaccga accgaatcgg tgagctcatg
      181 cggtattagc caacctttcg gttggttatc ccccacttcc gggcaggtca cttacgtgtt
      241 actcacccgt tcgccactag ctcacgggcc gaagcccgtg aacccgttcg acttgcatgc
      301 gtaaggcacg ccgccagcgt tggtcctgag ccaggatcaa actctccgac aaataccagc
      361 gccatccgaa gacgacgcgg ttgctcgaac agctgtcctt gagacagggt ccgccattgc
      421 gtcctttacc attcgctatc cagttgttaa ggtgccgccg agaacgagtc tcgacgcggg
      481 acacattaag acacaaacac gactgctcgc cctacccggg cggctgacgc gctgtgccaa
      541 ccagtagtgc cccgtcggta tgcttacctt atcaaggttc gcccgcgttg tcaacgggct
      601 gtaagtgtca tctcgacgct ttccctggcc ttggtggaca agaaaggcac atccgcttcc
      661 cgccgggcgg gttcgggtgt gccgttcgac tccccgttgc caatcatgac gatagcaccg
      721 caggctccgc tcgtcaagcc cgcatctcgc tcggctcgaa cgctatgcgc acgctgcctg
      781 ccggtgttcc cgaggggaac ggggtcgcgg ccaagcccga aacggcgagg atgtggcctt
      841 ccgcatcgac gatgaggggt acgcgatcgc gctcccagcg cggcaccttt gcgtcgacca
      901 agatgtcctg gaccttccgc gagcctctcg ccgtgcgcag gcgatcgccc ggccggcgag
      961 aacgggccca caacccccgc tccgctacac tcaccggcac gatgaccgcg gaatcgtcgc
     1021 cggccacccc gtcccatgca gccgctcgga cgatccacgg tcccacaacg gtctcgcccg
     1081 gaacggcgag ccgagtttcg ggaagggggc cggaggctga agcgccgccg gcgacctcga
     1141 tgcgcacgtc atgggcgagc cggacggctc gaacgccggc gggtaacgtc gcgctccggc
     1201 cggcgcggcc ggtaagcagc gaccgcagac gttcgagggc cgcggcgtcg gcgccaaggc
     1261 cggcccacac gagggcctgc gcgaggacgg ccgtgacgac ggcggcaggg agcgcggcga
     1321 gcacctcggc gcgcagaaag agggccccct ctcgctcttc cgtcgcttcg ggccaggcgc
     1381 gctccttctc ggtctcgatg agctgggccg cttcgcgaat actgctcgca gcgttggcca
     1441 tcgcggcgac cgccctcgga ttctcacgct cgagcagggg cacgaggtcc tgtcgaaccc
     1501 ggttgcgaag gtagcgccgg tcatcattcg tagggtcgcg gcgaggctga aggcagtggg
     1561 cctgcatata ggcctctgtc tccgcgcggc gaaccgacaa gagaggacga acaatatcgg
     1621 gacccggcgg cgaagcagga aacggccacg gtgcccgggg ggccatggcg cccaagcctt
     1681 gcagcccggc cccgcggagg agccgcatca gaacggtctc cgcctgatcg tccatggtgt
     1741 gtccgagggc gacgatgagc gcgcccgaac gtcgcgccgc tgtgaggagc gcagcatagc
     1801 gcgcttgccg cagcgcctct tcggacgagg aggcagggaa tgcacgctct gttagcagtg
     1861 gaacgcccgc ccgctcggca aggaatcgca cgaatcggag gtcggcatcc gtctcctccg
     1921 ggggccgcac gccatggtcg atatggaccg cggtcaaatc cagagggagc tccgcgcgtg
     1981 ctgccacgag ggcaaggagg agcgccgacg aatcggggcc gccggaaacg ccaaccagta
     2041 ctcgatcgcc gggccggaca aggcattccg tccgcagtgc ggcgaggacg cgatgttcga
     2101 acgcgcgcac gaggcgaagg ttccggtagt ctccggtgag ggactcgcga ggggcttcgg
     2161 gcataggcat gccgccctca gtggatgaac gagcgctgcc accacgtgcc atcggcgtcc
     2221 tcggcggact cccggggcgg ggagatgacg atgacgctgg gctcgcccgg catcacgagt
     2281 cctagctctc ggcgcgccgt cgtctcgatg tactcgtccg tggctagata gtcgcggatg
     2341 ccttggagct ggtcgtactg aacttggtac tccgcgatct cgcgttgcag cgcctcgcgc
     2401 tcgcggtcga ggctgtagtg gtcggtggca ttgctgaccg cggttgcgcc gaagtagatc
     2461 acgacgagcg cgataacggc gaatacgatg cgcgtcggcg tgatggcggg cacgcccttc
     2521 tccccaggct gatgctcgca tcaccgtagc acaagggatt ggtctacaca atcataccgt
     2581 tcccgcttgg aacggcggac ctcaacggtg ctgcctacgc ttctgtacag cgcgttctac
     2641 gcgtcgccct ggcgagcatc aacgacgccg cggcgccaga aggcggccat cggtggaagg
     2701 cggccgccgt acaggtgaca agtctcgttc agccggacca cacgcgtccg ctccggcgtg
     2761 aggtccagtg catggcacgc cgcgacgtct tggcgtatgc gacggaagtc ggcgaggtcg
     2821 atgtcgaggg cacggcagat gagcgtcgcg atgacgaagt tgtggctgac cagcacgacg
     2881 acgccgctcg catgttcgtc catcagccgc tcgacggtgc gccatgcccg gtcctgtacc
     2941 tgtgcgaggc tctcgcctcc cggcataggt gtcgtcgcgg cgccgggccc catccaggcg
     3001 cgcaggaact cggcatgctg ttcgcgcatc tcccgcgtgc tgagtccgtc catcacgcct
     3061 acatccatct cgatgaggtc gggctccggc tccaccgcaa gcccgtgccg tccggcgatg
     3121 gcctcggccg tctccatggc gcggcgaagc gggctgctgc gcaccgcaac gacggattcg
     3181 tgggcgaggg cttgcgcgac ggcaacgcat tgcgcgcggc ccgtctcgtt cagcggattg
     3241 tcggcgcgac gctgggcgaa cccgtcgcga ttgtggtcgg tctcggcgtg gcgaacgagg
     3301 atgagacgca cggtgcccct tagctctgtt gcgttaggac gagcgactgt tcgccatttc
     3361 gagcgcccgt gtcgcgtcct cgccgatgcg atcgagtcgg tcgagggccc attcgcacca
     3421 ttcgatgcag gtacgctcgt agcgcagggc ccattccagc gtcagcaccc agtagttcgg
     3481 gtcgggcatg gtgggattgc gcgccacaat gtcgcgtagc gattcccagt tcgacgtttc
     3541 gtagtagcgg agacgctcgc ggtgggcgtc gagctgctcg ttcaggtgtt gccgcatgag
     3601 ctccggctgc aatgcggccc cgaaaaacac cttgatgagg aaggcatcct tcatgatggg
     3661 gaggtcggtg ggttcctgca tccactcgat gagcgcggcg tggccggcgt ccgtcagctc
     3721 gtagaccttg cggttcggcc ggtcgctctg cggctcgatg tgcatggtca gcaagccatc
     3781 gcgttccatc tgcttgagag ccgggtagat ctggctcagc tcagcgttcc agaagaagtg
     3841 gaccgtggaa tcgaatgcct gcttcagctc gtagccggtc atgggcttat agctaaggaa
     3901 gcccagaagg gcgtgtttca tcgacatcga tgtaaacctc tagggcttca gtatagtgcg
     3961 cctggcccgc cttgcaaaac ttacccgcgt taaggcaaag ggtgaggacg ctcagggttc
     4021 ggggaaggcc cgcagcagga ggtcgcgagc cgcagtgtgg ggatcaaccc tccgctcggc
     4081 gacgcgctgg accgccgcgt ccagtgcgcc atcggccacc gaccgcagga accgctgtgc
     4141 gagct
//
LOCUS       DATMBB010000091         4626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_149979, whole genome shotgun sequence.
ACCESSION   DATMBB010000091 DATMBB010000000
VERSION     DATMBB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149979"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /gene="carB"
                     /locus_tag="VNL92_01940"
     CDS             complement(<1..1916)
                     /gene="carB"
                     /locus_tag="VNL92_01940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWO93503.1"
                     /translation="MTQKPQKVLIIGSGPIVIGQAAEFDYAGTQACRAMREEGVHSVL
                     VNSNPATIMTDEGIADTVYIEPLTVEAIGRIIEQERPDGLLPTLGGQTGLNLAVALAE
                     AGILDQYSVRVLGTPIDTIRRAEDRELFKRMLEEIGEPVLEGETCTSWEAAQRAAQRI
                     GLPLVIRPAYTLGGTGGGFVTTWEEFEAIARAGLAASPIGQILVERSLAGWKEIEYEV
                     MRDSAGNCITVCNMENFDPLGVHTGDSIVVAPSQTLSDHDYQMLRTAALKIIRALGIE
                     GGCNIQFALAPRPDIVPWADPDDVPERLPYYVIEVNPRVSRSSALASKATGYPIARVA
                     AKIAVGRTLPEITNAVTQKTTAAFEPALDYVVVKIPRWPFDKFPFGDRSLGTQMKATG
                     EVMAIDRTFEAALQKAIRSLELGARSVLWEEPAWADHAVERPLGPTDERLWSLLAALR
                     RGADPVVLARSTGIDPWFLDRLFAIVAMERRLLSEPLTRDLLREAKRMGFSDADIGQL
                     ADRLPDQVRQDRQRWNIRPVYKMVDTCAAEFEAATPYFYSTYEEENEATTLDGQRALV
                     IGSGPIRIGQGIEFDYCSVHAATALREAGYRAIMANSNPETMSTDFDTSDRLYFEPLD
                     EEAVRDILDNEGAGG"
     gene            complement(1939..2403)
                     /locus_tag="VNL92_01945"
     CDS             complement(1939..2403)
                     /locus_tag="VNL92_01945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93504.1"
                     /translation="MWKVTQPRIFRARISDADAIHQVLNGVAAEGGPERNGIEGLVTP
                     DSVAQWMRDLGDRGALFVARVGERGVGAAALEPAGEPGAARLRVWVLPAYRRQGIGTE
                     LGRVATQLARDLGYVRVLGHIPEQNEAALSYFSSVAGLENLQPGLRFELPLA"
     gene            complement(2407..3570)
                     /gene="carA"
                     /locus_tag="VNL92_01950"
     CDS             complement(2407..3570)
                     /gene="carA"
                     /locus_tag="VNL92_01950"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWO93505.1"
                     /translation="MASALLVLEDGTVFRGESFGARGHVHGEVVFNTSMAGYHEILTD
                     PSYAGQIVTMTYPLIGNYGIADVDSESRRVQVRGFVVRQAMNGPSHPHARESLPAYLA
                     EAGICGISGIDTRALTRRLRMRGVLMGTITTEETAEEALQRLRGAIRYDSEPLVEGVT
                     TPDMYHWEGREVDGCASERAPGPHIVVVDLGVKYNILRMLRAMGCHITTVPASTSASD
                     IFALRPDGVVLSPGPGDPALLDDVVETARMLAGRSPLMGICLGHQVLGRAFGAKTYKL
                     KFGHRGGNHPVQDLETGLVHITSQNHGYAIDADGLKAGAEVSHVNLNDGTVEGIRHRS
                     LPIVSIQYHAEGSPGPEDNRYLFERFLRFVSEASPRRSGSAASALAYQGGGRT"
     gene            complement(3563..>4626)
                     /locus_tag="VNL92_01955"
     CDS             complement(3563..>4626)
                     /locus_tag="VNL92_01955"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWO93506.1"
                     /translation="GGFGTICAMPNTDPPADSRATIDYLLRTAAEGPARVLPIGAITK
                     GRAGGELAELGDLADAGAIAFSDDGDCLANAGLMRSALSYAAALNRPIIQHAEDVALS
                     KGAPAHEGWVATRLGLAGQPAAAEESIVARDIDLVALTGGRLHLAHVSTAGAVEHVRR
                     AKERGLPVTAEVTPHHLTLTHEVLLEQPYDTNTKVNPPLRSAEDVAALREALRDGTID
                     AIATDHAPHALEDKHCEYDVAAFGISGLETAFGVAMTLVHERVISLDRLIEALTIGPV
                     RALGLDRSSGVPGLGTLAEGAPGDIALIDPETAWTVDPANFVSLGKNSPFAGRQLRGR
                     VVATVHDGRVVYESRVSVG"
BASE COUNT          754 a         1503 c         1546 g          823 t
ORIGIN      
        1 cctccggcgc cctcattgtc caggatgtcg cgcacggcct cctcgtccag aggctcgaag
       61 tagagccggt ccgatgtgtc gaagtcggtg gacatcgtct cggggttcga attcgccatg
      121 atggcgcgat agccggcctc gcgaagggcg gtcgcggcgt ggacgctgca gtagtcgaac
      181 tcgatccctt gcccgatgcg gattgggccc gacccgatga cgagggcgcg ttggccgtcc
      241 aacgttgtcg cctcgttttc ttcctcgtag gtgctgtaga agtatggggt tgcggcttcg
      301 aactccgctg cgcacgtatc gaccatctta tagacgggac ggatgttcca gcgttggcga
      361 tcctgtcgta cctggtccgg cagacggtca gccaactgac cgatgtccgc gtcggaaaag
      421 cccatgcgct tcgcctcgcg gaggaggtcg cgggtcagcg gctccgagag gaggcggcgc
      481 tccatcgcga cgatagcgaa aagccgatcg aggaaccacg gatcgatccc ggtgctgcgc
      541 gcgaggacga ccgggtcggc gccgcggcgc agggcggcca gcagcgacca tagccgctca
      601 tcggtggggc cgagggggcg ctccacggcg tggtcagccc aggccggctc ctcccataag
      661 acagaccgcg cccccaactc gagggaccgg atcgccttct gaagcgcggc ctcgaaggtg
      721 cgatcgatcg ccatgacctc gccggtcgcc ttcatctggg tgccgagcga tcggtcgccg
      781 aacgggaact tgtcgaaggg ccaccgcggg atcttgacaa ccacgtaatc gagcgccggc
      841 tcgaaggcag ccgtcgtctt ctgggtgacg gcattcgtga tctccggcaa cgtgcgaccg
      901 accgcgatct tcgctgcgac ccgcgcgatg gggtagccag tcgccttgct cgcgagagcg
      961 gagcttcgcg agacgcgcgg attgacttcg atgacatagt aaggcaggcg ctcgggcacg
     1021 tcgtcggggt cggcccaagg gacgatgtcg gggcgcggcg cgagcgcgaa ctggatgttg
     1081 cagccaccct cgataccgag cgctcggatg atcttgaggg cggccgtgcg aagcatctgg
     1141 tagtcgtggt cggaaagcgt ctggctgggg gccacgacaa tgctatcgcc tgtgtggacg
     1201 ccgagcggat cgaagttttc catgttgcaa acggtgatgc agttgccggc ggaatcgcgc
     1261 atcacctcat actcgatttc tttccagcct gcgaggcttc gctcgacaag gatttgtccg
     1321 atcgggctcg cggcgaggcc ggcccgcgcg attgcttcga actcctccca cgtcgtcacg
     1381 aagccgccac ctgtgccgcc gagcgtgtac gccgggcgta tgacgagggg aaggccgata
     1441 cgctgggcgg cgcgctgagc ggcctcccac gatgtgcacg tctccccttc aagcacgggc
     1501 tcgccgatct cttccagcat gcgcttgaac agctcgcgat cctctgcgcg gcggatcgtg
     1561 tcgatcggcg tgccgagaac acggacgctg tactggtcga ggatgcctgc ttcggccagg
     1621 gctacggcca aatttagccc tgtctggccc ccgagcgtcg gcagcaaccc atcggggcgt
     1681 tcctgttcga tgatgcggcc gatggcctcg acggtgaggg gctcaatgta gacggtgtcg
     1741 gcgatgccct cgtccgtcat gatcgtcgcc ggattcgagt tgacgaggac ggagtgaacg
     1801 ccttcctcgc gcatggctcg gcacgcctgc gtgccggcat agtcgaactc agctgcctgc
     1861 ccaataacga tcggcccgga gccgatgatg aggaccttct gtggcttctg cgtcatttgt
     1921 cgttcgcttg cggagtgttc acgccaaggg cagctcgaag cggagtcctg gttgcaggtt
     1981 ctccaggccg gcgactgaac tgaagtagga cagggccgcc tcgttctgct cgggaatgtg
     2041 gccgagcacg cgaacgtacc ccagatcgcg cgccagctgc gtcgcgaccc gccccagttc
     2101 ggtgccgata ccctgccggc gataggccgg gagcacccac actcgtagac gtgccgcgcc
     2161 cggctcgccg gctggttcta acgccgcagc gccgacccct cgctcgccta cccgcgccac
     2221 gaagagcgcg ccgcgatcgc cgagatcgcg catccactgt gccacgctgt ccggcgtcac
     2281 gagcccttcg atgccgttcc gttcgggtcc gccttcggcc gcgacgccgt tcagcacttg
     2341 atggatggca tcagcatccg agatccgggc tcgaaagatt cgtggttggg tgactttcca
     2401 catccctcag gttctccccc cgccctggta ggcgagcgcg ctggcagcgg agccagaacg
     2461 tcgtggtgat gcttcggaga cgaagcgcag aaagcgctcg aaaaggtacc gattgtcctc
     2521 cgggccgggc gagccttcgg catggtactg gatggagacg atgggcaagg agcgatgtcg
     2581 gattccctcg accgtgccgt cgttcaggtt gacgtggctg acctccgcgc cagccttgag
     2641 tccgtctgca tcgatggcgt atccgtggtt ctgggacgta atgtgcacaa gccctgtctc
     2701 caggtcttgc actggatgat tgccgccccg gtgcccgaac tttagcttgt aggttttcgc
     2761 gccgaacgcg cgcccgagga cctggtgtcc gaggcagatg cccatgaggg gcgaccggcc
     2821 ggcgagcatg cgcgccgtct caacgacgtc gtcgagaagc gcggggtcgc ccgggccagg
     2881 agacaggacg acgccatccg gccgcaacgc gaagatgtcg gaggcggatg tcgaagcggg
     2941 aacagtggtg atgtgacagc ccatcgctcg cagcatccgc aggatgttgt acttcacacc
     3001 cagatcgacg acgacgatgt gtggacccgg agcacgttca ctggcacaac catcgacctc
     3061 gcgtccctcc cagtggtaca tgtccggcgt cgtcactccc tcgacgagag gttcgctatc
     3121 gtagcgaatc gcgccccgaa ggcgctgaag cgcctcctcg gccgtctcct ccgtcgtaat
     3181 agtgcccatg aggacgccgc gcatgcggag ccgcctcgtc agcgcacgtg tatcgatgcc
     3241 gcttatcccg catatgccag cctcggcgag ataggcaggc aggctctccc gcgcatgagg
     3301 gtggcttggg ccgttcatcg cctgccgcac gacaaagccc cgtacctgga ctcgccgcga
     3361 ctcagaatcg acgtccgcga tcccgtaatt gccaatgagt ggatacgtca tcgtgacgat
     3421 ctgccccgca taggaggggt ccgtgaggat ctcgtggtag ccggccatcg atgtgttgaa
     3481 cacgacctcg ccgtgcacgt ggcctcgggc gccgaagctc tcgccccgga acaccgtccc
     3541 gtcctcgagt acgagcagcg cgctagccaa cggacacccg gctttcgtag acgacgcggc
     3601 catcgtgtac cgtcgccacg acgcgccccc gcagctgtcg tccggcgaaa ggcgaattct
     3661 ttccgagcga gacgaagttg gccgggtcga cggtccatgc ggtctccgga tcgatgaggg
     3721 cgatatcgcc cggagcgccc tcggcgagcg tgcctaagcc gggcacgccg gacgaccggt
     3781 cgagccccaa cgcccgtacc ggcccgatgg tgagcgcttc gatgagacga tcgagcgaga
     3841 tgacccgctc gtgcacgagc gtcatggcca ccccgaaggc ggtctcgagg ccgctgatgc
     3901 cgaaggcggc gacgtcgtat tcgcaatgct tgtcttcgag cgcgtgcggc gcgtggtcgg
     3961 tggcaatggc gtcgatcgtt ccatcccgca atgcttctcg aagcgcggcc acgtcctcgg
     4021 ccgagcggag cggcggattg accttcgtgt tcgtatcgta cggttgctcc aggagcacct
     4081 cgtgcgtcag ggtcaaatga tgcggcgtaa cctccgcagt cacagggagg ccacgttcct
     4141 tcgcccgccg tacgtgttcg acagcgcccg ccgtgctcac gtgggccagg tggaggcgtc
     4201 cgccggtcag ggccacgaga tcgatgtcgc gcgcgacgat gctctcctcg gcagctgctg
     4261 gctgtccggc gagacccaga cgcgtcgcca cccatccttc gtgtgctggg gcccccttcg
     4321 agagcgccac gtcttcggca tgttggatga tagggcgatt gagcgctgca gcgtagctca
     4381 aggccgagcg catgaggccg gcattggcca gacagtcgcc gtcgtcgctg aacgcgatgg
     4441 cgcccgcatc ggctaggtcg ccgagttccg cgagctcgcc gccggcacgc cccttcgtga
     4501 tggcgccgat gggcagcacg cgggccgggc cctcggcggc ggtgcgcagc agatagtcga
     4561 tcgtggctct cgagtccgcg ggcgggtcgg tattgggcat cgcgcagatc gtgccgaacc
     4621 cgccgt
//
LOCUS       DATMBB010000092         5743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_106739, whole genome shotgun sequence.
ACCESSION   DATMBB010000092 DATMBB010000000
VERSION     DATMBB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106739"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VNL92_01960"
     CDS             complement(<1..359)
                     /locus_tag="VNL92_01960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWO93507.1"
                     /translation="MNLTDQTATLMAGETPVWTALVTSGREGWETPTGTFYIGLRVEN
                     ETMTSESLGISPEDEFYHLEDVLFTQYFTNEGHALHLNYWQPEYVFGNTPTSHGCIGM
                     FYDDALYFWQALDIGSRV"
     gene            829..1881
                     /gene="glnII"
                     /locus_tag="VNL92_01965"
     CDS             829..1881
                     /gene="glnII"
                     /locus_tag="VNL92_01965"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase GlnII"
                     /protein_id="HWO93508.1"
                     /translation="MGDTLGYYKAEYIWIDGTQPTSKLRSKGKVVPLGEEPPIWGFDG
                     SSTMQAEGHDSDRVLKPVFVCPDPVRGGENKLVLCEVLMPDMTPHESNTRHLCAEVHA
                     KYARHEPWFGIEQEYTFFDGAKPLGWPDNGFPAPQGGYYCGVGADEVFGRDIVEAHLD
                     ACIRAGLAISGINAEVMPAQWEFQIGPAAGVGAGDQLWVARWLLYRIAEDFGVSATLY
                     PKPVKGDWNGAGAHTNFSTKEMRESWEPVVTAAEALGLRHDLHIANYGHAIEERLTGL
                     HETCSYKEFRYGISDRGASVRIPWQVARDRKGYIEDRRPNANCDPYIVTRLITESVCS
                     VFEEKGIGATAAAATA"
     gene            1988..2176
                     /locus_tag="VNL92_01970"
     CDS             1988..2176
                     /locus_tag="VNL92_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93509.1"
                     /translation="MGDPDGEWHYSTEGELDPDLTEEAGYADWDPPQRRWLAVVLWVT
                     ALLLAAAVAAGVILTALA"
     gene            complement(2393..3226)
                     /locus_tag="VNL92_01975"
     CDS             complement(2393..3226)
                     /locus_tag="VNL92_01975"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258964426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWO93510.1"
                     /translation="MENAAYTRPELLAEPDWLWQHREDPNVRVIDTGAPAAYERAHIP
                     GAVALGPNGFLKRDDGVLVLPADDFSELMQALGVSAGTTVVAYSDSDSPNAARLWWTL
                     AYYGHTNAKVLNGGWSRWLTEGWPVTIDRPAVQRGNFEARPNGSLLCTLDELQVALGR
                     EDAQVLDVRSKGEWLGTNSRGNARVGHVPGAAHLEWTRTLSDDASKRVRPAAELRAML
                     AEAGIEPGREVITYCQGGIRAAHMAFVLALLGYDRVRNYDGSMREWANRDDTPLVVEQ
                     A"
     gene            complement(3289..4644)
                     /locus_tag="VNL92_01980"
     CDS             complement(3289..4644)
                     /locus_tag="VNL92_01980"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWO93511.1"
                     /translation="MGWVSDANAALFTDLYELTMLQAYYREGMVEPAVFDLFVRELPE
                     ERNFLVAAGLDDALTYLERLSFSEDALSYLQSLGLFDDGFLRRLADFRFTGNVFAVPE
                     GTPVFANEPLVEVEAPMPEAQFVETFLLNQVTFQTMIASKGARAVIAAGGRPVIDFGT
                     RRTHGADAGLKAARALAIAGFASTSNVLAGHMYGLQVSGTMAHSYVSAHPTELDSFRA
                     FARSYPETILLVDTYDTEEGVQNVIRLARELGESFRVTGVRLDSGDLTELSQRARRML
                     DAAGLSSVRIVVSGGLDEYSVARLMEAGAPIDSFAVGTAVGTSADAPRLDTAYKLVAY
                     AGEGRMKLATAKRTLPGRKQVYRHRDGDGRIRRDVLALASEQLDGEPLLREVMRNGTR
                     LAQGRSSLQDARELAERELAALPEELRSLTPARVPYPVELSAELQSATEALAQKLRIH
                     H"
     gene            complement(4659..5249)
                     /locus_tag="VNL92_01985"
     CDS             complement(4659..5249)
                     /locus_tag="VNL92_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWO93512.1"
                     /translation="MAEPAYDRSTALLVVDVQNDFADPGGSLYVRGGEEVVPIANREI
                     ASARAAGALVVYTQDWHPESTPHFQKDGGIWPVHCVHDTWGAAFSAGLDVRGEIVRKG
                     VGGEDGYSGFTVRDPGSGAEHPTVLEQLLRSRGIRRIVLLGIATDYCVRETALDALRL
                     GFDVTVLREGIRGVDLQQGDSERALAAIVAAGGRIV"
     gene            5339..>5743
                     /locus_tag="VNL92_01990"
     CDS             5339..>5743
                     /locus_tag="VNL92_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93513.1"
                     /translation="MATARERAGGAETRTLASSGLPRDLQGLTWRDARARAERGLANV
                     DTSQLPANGDVVRRNVLTLFNIILGSLILALFALAVADRDVGHFQDGIFVGVIVAANV
                     AVATFQEIRATNRLRQLRALTVPRATVVRDGED"
BASE COUNT          999 a         1916 c         1875 g          953 t
ORIGIN      
        1 actcgagagc cgatatcgag cgcctgccag aagtagagcg cgtcatcgta gaacatgccg
       61 atgcacccgt ggctcgtcgg cgtgttgccg aagacgtatt cgggctgcca gtagttgagg
      121 tgcagagcgt ggccctcgtt cgtgaagtac tgcgtgaaca gcacgtcctc gaggtggtag
      181 aactcgtcct ccggcgagat gccgagcgat tccgaggtca tcgtctcgtt ctctacgcgc
      241 aggccgatgt aaaacgttcc ggtcggcgtt tcccatcctt cacgaccgct ggtgacgagg
      301 gcagtccaga ccggcgtttc gccggccatg agcgtcgctg tttggtccgt caggttcacg
      361 tcgacccagc gctcgccgac cagctccgat atgggcggac gaacgggcgc cggcggcggc
      421 ggtggaggtt cgggagtggg cgtggggctc ggtgtcggac ttggggttgg gctgggcgtc
      481 ggcgttggac ttggcgttcg cgtaggggta ggggaaggtg tggcggtcga ggaaacgaca
      541 cggaccgagc ttgcggggcg gtcctcgcca tcgatcgcga gaccgacagt aacaccgcca
      601 ccgacagcgg ccgcgaggac cgccgcgacg ccgagcaacc ccagccaccg ccccccgaat
      661 ttcaggtgac gcccccatct tgcaaatgtc cgcctgagtc taacataatc ccgttcttgc
      721 gtgaccagcc agatgcggac cacggcaaaa aagtttgcga gaggtcggcg cgtgaaactt
      781 ttctgaaata ttgcggctgt atgctacggg caacagtcct acgaaaggat gggggacacg
      841 ttgggttact acaaagcgga gtacatctgg atcgacggca cgcagcctac gagcaagctg
      901 cgctccaagg gtaaggtcgt gccactgggc gaggagccgc ccatctgggg attcgacggc
      961 tcgagcacca tgcaggcgga aggccacgat tccgatcgcg tcctgaagcc cgtgttcgtt
     1021 tgcccggacc ccgtccgcgg cggcgagaac aagttggtgc tgtgcgaagt cctcatgccc
     1081 gacatgacgc cgcacgaatc caacacgcgc catctctgtg ccgaggtgca cgcgaagtac
     1141 gcgcgccacg agccgtggtt cggcatcgag caggaataca cgttcttcga tggcgccaag
     1201 ccgctcggct ggccggacaa cggattcccc gcaccgcagg gaggctacta ctgcggtgtc
     1261 ggcgccgacg aggtcttcgg gcgagacatc gtcgaggccc acctcgatgc ctgcattcgg
     1321 gccggcttag cgatctcggg catcaatgcc gaagtgatgc ccgcgcagtg ggagttccag
     1381 atcgggcccg ccgcgggcgt aggagccggc gaccagctct gggtggcgcg ctggctcctc
     1441 taccgtatcg ctgaggactt cggtgtcagc gccaccctgt accccaagcc agtcaagggc
     1501 gattggaacg gcgcgggcgc tcacaccaac ttctcgacta aggagatgcg cgagtcctgg
     1561 gagcccgtgg tcaccgccgc cgaagcactc ggcctccgcc acgacctcca tattgcgaac
     1621 tacggacacg ccatcgagga gcggctcacc ggactgcacg agacctgctc gtacaaggag
     1681 ttccgctacg gcatctccga tcgcggcgcc tctgtccgca taccctggca ggtcgcgcgg
     1741 gaccgcaagg gctacatcga ggaccggcgc ccgaacgcga actgcgatcc gtacatcgtg
     1801 acgcgcctca tcacggaatc cgtgtgctcg gtcttcgagg aaaagggcat cggcgctacg
     1861 gcggcggccg cgaccgcgta gctccgaaca cgatacatcg caaacagggg gcgtccttct
     1921 tccgtggaag gacgccccct gttgattgcg cttgaccaag ggggcacgtt gcgtcaacga
     1981 tgcgtgcatg ggcgaccccg atggcgaatg gcattactcg acggagggcg agctcgaccc
     2041 cgacctcacg gaagaggccg gctatgccga ctgggacccg ccacagaggc gctggctcgc
     2101 cgtcgttctc tgggtaaccg cgctcctgct cgccgcggcc gtcgccgctg gcgtcattct
     2161 gacggcgctg gcctgatctc ggccccttcc tccgctcgga cgggtcgctt cgccaatggc
     2221 ggcgattcgg cagccggtcg gaacccagcg cagtcggaca cggggaggcg tggatagcgg
     2281 gggaaccggg gttcgagcgc cgagagctgg cagaggatga agcacgagcc tcgggccgag
     2341 gagatccggc gagcgtggag gcaccgaccg cagaggcctt cgccggtctc cgtcacgctt
     2401 gctccaccac gaggggcgtg tcatcgcggt tcgcccactc gcgcatcgag ccatcgtagt
     2461 tacgcacgcg atcgtagccg agcagcgcca gcacgaacgc catgtgcgcg gcgcggatac
     2521 ctccctgaca gtacgtgatc acctcgcggc ccggctcgat gccggcctcc gcgagcatcg
     2581 ctcggagctc ggcggcgggc cgcacgcgct tcgacgcgtc gtcagacaaa gtgcgggtcc
     2641 attccaggtg cgcggctccc ggcacatgac ccacgcgcgc attgcctcga ctgttcgtgc
     2701 cgagccactc ccccttgctg cgaacgtcca gcacctgcgc atcctcgcgc ccgagcgcta
     2761 cctgcaattc gtcgagcgtg cagagtaggg atccattggg gcgagcctcg aagttgcctc
     2821 gctgcaccgc cgggcggtca atggtcacgg gccagccttc cgtcagccaa cggctccaac
     2881 ccccattcag caccttggcg ttcgtatgtc cgtagtacgc caaggtccac cagagccgtg
     2941 cagcattggg gctatcgctg tcggaatagg caacgacggt cgttcccgcc gatacgccga
     3001 gcgcttgcat cagctcggag aagtcgtcgg caggaagcac gaggacgccg tcgtcccgct
     3061 tgaggaagcc gttggggccg agcgctacag caccggggat gtgagcgcgc tcgtaggccg
     3121 cgggcgctcc cgtgtcgatg actcgcacgt tcgggtcctc gcggtgctgc caaagccagt
     3181 ccggctcggc gagcagttct ggtcgcgtgt aggccgcgtt ctccatcccc cctcctcgcg
     3241 cccagctccg cctctgtctt cggctgaggg catgatactg ggccttggtc agtggtgtat
     3301 acgcaacttc tgcgcgagcg cctcggtggc cgactggagc tccgcgctta gctcgaccgg
     3361 atacggcacc cgcgcgggcg tgagcgagcg gagctcctcc ggcagggctg ccagctcccg
     3421 ttcagccagc tctcgcgcgt cctggagcga actccttccc tgcgcgagcc tcgttccatt
     3481 ccgcatcacc tcgcgaagca gtggttcgcc atccagctgt tcgctcgcga gcgcgaggac
     3541 gtcgcgccgg atccggccat cgccatcacg atgccgatag acctgcttcc gtccgggcag
     3601 ggtccgcttc gccgtcgcta gcttcattcg gccctcgccg gcatacgcga cgagcttgta
     3661 cgccgtatca agccgaggcg catcggccga tgttccgaca gccgtgccga cggcaaacga
     3721 atcgatcggc gcccccgcct ccatcaacct ggcgacgctg tactcgtcga ggccgccgct
     3781 cacgacgatg cgcacgctgg acaagcccgc cgcgtcgagc atgcgacggg cccgctgcga
     3841 cagctccgtc agatcgcccg aatcgagccg cacgcctgtg actcgaaacg attcgcccag
     3901 ctctcgcgcc agccgaatca cgttctggac gccctcctcg gtatcgtagg tatcgacgag
     3961 aaggatggtt tcggggtagc tccgagcgaa cgcgcggaag gagtcgagtt ccgtggggtg
     4021 tgccgagacg tagctgtggg ccatcgtgcc actgacctgc aggccgtaca tgtggccggc
     4081 gagcacgttc gatgtcgagg cgaagcctgc tatcgcgagc gctcgggccg ccttcagccc
     4141 cgcatcggcg ccgtgcgtcc ggcgggtgcc gaagtcgatc acgggccggc cgccggcggc
     4201 gatgaccgcc cgcgccccct tcgaggcgat catcgtttgg aacgtcacct gattcaggag
     4261 aaaggtctcg acaaattgtg cctcgggcat cggcgcttca acctcgacca gaggctcatt
     4321 ggcgaacacc ggcgtcccct ccggaacggc gaagacattg cctgtgaatc gaaagtctgc
     4381 cagtcggcga agaaagccat cgtcgaacag cccgagcgat tgcagatagg acagagcgtc
     4441 ctccgagaag gacaatcgct caaggtaggt cagggcgtca tcaagccccg cggccacgag
     4501 gaagttgcgt tcctcgggca gctcccgcac gaacaggtcg aagacagcgg gttcgaccat
     4561 gccctcgcgg tagtaggcct gcagcatggt caattcgtac aggtcagtga agagcgcggc
     4621 gttcgcgtcg cttacccagc ccacgtcgtg tcccgccgct agacgatgcg gccgcccgcg
     4681 gcgacgatgg ccgcgagcgc gcgctcggag tctccctgct ggagatcgac tcctcgaatg
     4741 ccctcccgaa ggaccgtcac gtcgaagcca agccgaagtg cgtcaagcgc cgtctctcgt
     4801 acgcagtagt ccgttgcgat gccaagcagc acgatgcgac ggatcccccg cgaccgcaac
     4861 agctgctcga ggacggtcgg gtgctcggcg ccggaacccg gatcgcgcac ggtaaagccg
     4921 gagtacccat cctcgccacc cacgcctttg cgaacgatct ccccgcgcac gtcgaggccg
     4981 gcgctgaagg cggcgcccca cgtatcgtgg acgcagtgca cgggccagat cccaccgtcc
     5041 ttttgaaagt gaggggtact ctccgggtgc cagtcctgcg tatagacgac gagcgcacca
     5101 gcggcgcggg cgctggcgat ctcccgattg gcgatcggaa cgacctcctc accaccgcgc
     5161 acgtagagac tgccgccggg atcggcgaag tcattctgca catcgacgac gaggagagcc
     5221 gtcgagcggt cgtaggccgg ttccgccatc aacaagtcct cacttggtcg ggagacgcgt
     5281 accctcacta taccaagggg aggcgtgtgc gataatcggt cagcgggagg agcctggctt
     5341 ggcaacagca cgggagcgag cgggcggagc cgagaccagg acgcttgcca gctccgggtt
     5401 accgcgagat ctccaagggc tcacgtggcg cgacgcacgg gccagggcgg agcgcggcct
     5461 cgccaacgtc gacacgtcgc agttgccggc gaacggcgac gtcgttcgcc gcaatgtcct
     5521 cacgcttttc aacatcatcc tcggctcgct catcctggcg ctcttcgcac tcgccgtagc
     5581 cgaccgtgac gttgggcact tccaggatgg catcttcgtg ggcgtcatcg tcgcggcgaa
     5641 cgtcgcggtc gccacatttc aggagatccg cgccacgaac cgtctccgtc aactgcgagc
     5701 gctgaccgtg ccccgcgcta ccgtcgtgcg tgacggggag gat
//
LOCUS       DATMBB010000093         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_348429, whole genome shotgun sequence.
ACCESSION   DATMBB010000093 DATMBB010000000
VERSION     DATMBB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348429"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..519)
                     /locus_tag="VNL92_01995"
     CDS             complement(49..519)
                     /locus_tag="VNL92_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HWO93514.1"
                     /translation="MIAGPRLLRRLATAVLLVALAGMVSTEQRQAAAGPPLQCTTDCY
                     VATTGSDSNDGSQASPFLTIQKALDTVQDGGTVHVAPGAYDAMTRNTIDKNVTLLGAR
                     AGVDPVGRDPTDASTESIVRGDPGFQVTADVNVTIDGFTFQELDSSTAIITAEE"
     gene            705..1853
                     /locus_tag="VNL92_02000"
     CDS             705..1853
                     /locus_tag="VNL92_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWO93515.1"
                     /translation="MAGLSERDLERLRAFLEGAEPITSLPGFVAYVVGSLPSLVRCNA
                     IAYSEIDVESRRAESVFEPAALLFEGAHEAFQAHVDQHPLVRRVRETGDGRAYRISDF
                     LTEDEFHQLDLYQQFFCRIGAEDQIGFALPAPPPLVVGIALNRERRDFPERDRMLLNL
                     ARPYLALLHDRIQLHGWAAGMVDGLQRGDDRWGSAVILLGRRGEMEFRNAAAARLLDA
                     YFSGAGGPATALPRELAHWVAGQRAVSARRFESPPPAEPLVRLREGKRLVVRYAPALR
                     PNEREVLLLEETPEAALAASPLMELGLTEREGQIAVILASGMTNAEIGSALGISEKTV
                     RTHLERIYEKLRVANRAAAVAFVYDAQHRQDEAEPAAGHALGPRAVLG"
     gene            1977..2408
                     /locus_tag="VNL92_02005"
     CDS             1977..2408
                     /locus_tag="VNL92_02005"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWO93516.1"
                     /translation="MPTRSAQAEWRGDLQQGRGSMWVGEGTEVVYTSDSRFESGSGGN
                     PEELIAAAHAGCFSMALSAALARAGFPPTRIRTKASVRLERLESGFGITGINLETEGE
                     VPGIDEATFRREAEGAKQNCPVSKALAAVPEITLTARLVQG"
     gene            complement(2496..2571)
                     /locus_tag="VNL92_02010"
     tRNA            complement(2496..2571)
                     /locus_tag="VNL92_02010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2536..2538),aa:Arg,seq:tcg)
BASE COUNT          407 a          886 c          982 g          465 t
ORIGIN      
        1 gaagatgttg ttctgcacgg tcaccatcgc gcccacgtcg aacgcaccct actcttctgc
       61 ggtgatgata gccgtggagg agtccaactc ctggaaggtg aagccatcga tcgtgacgtt
      121 cacgtcggcg gtgacctgaa accccgggtc gcccctcacg atcgattccg tcgatgcgtc
      181 ggttgggtcg cggccgaccg ggtccacgcc tgcccgcgct ccgaggagcg tgacgttctt
      241 gtctatcgtg ttgcgggtca tcgcatcgta ggcgcctggc gccacgtgca ccgttccgcc
      301 gtcctggacg gtgtcgagcg ccttctggat cgtgaggaag ggagacgctt gcgagccgtc
      361 gttgctgtcg ctcccggttg ttgcgacgta gcagtcggtc gtgcactgca ggggaggtcc
      421 cgccgcagcc tggcgctgtt ccgtggagac catgcctgcc aaggccacga ggaggactgc
      481 tgtggccagc cgccggagaa gtcgtggtcc ggcaatcatg ctccgtgcct ccctccagac
      541 cgtcgcgaac gcgcacacta cgcagcgagg ctgcggtcga tagtcgtccg ccgcgccgat
      601 actacgtgac gcagcgggac gcggccatac gacgaatgtc gtatacttag cccaatcctg
      661 ggcggaggga ctcgggcttc cctccgccgt tcggtgcggg ggccatggcg gggctttcgg
      721 aacgcgatct ggaacggctg cgggcctttc ttgagggcgc agagccgatc acttcgctgc
      781 ccggcttcgt cgcgtatgtc gttggttccc tcccttctct cgtgcgctgc aacgccatcg
      841 cctacagcga gatcgacgtg gagagtcggc gcgccgagtc cgtgttcgaa ccagcggcgc
      901 ttctctttga aggggcacat gaagccttcc aagcgcatgt cgaccagcac cccctcgttc
      961 ggcgtgtccg ggagacgggc gatggccgcg cctatcgcat ctcggacttc ctcaccgagg
     1021 acgagttcca ccagctcgac ctgtaccagc agttcttctg tcgcatcggc gccgaggacc
     1081 agatcgggtt cgcgctgccc gcgccgccgc cgctcgttgt gggcatcgcg ctcaatcgag
     1141 agcgccgaga cttccccgag cgggatcgca tgctcctgaa tctcgcccgg ccgtatctgg
     1201 cgctcctgca tgatcgcatt cagctgcacg gctgggctgc gggcatggtc gacggcctgc
     1261 agcggggcga cgatcgctgg gggagcgcgg tgatcctgct cgggcggcgt ggagagatgg
     1321 agttccggaa cgcggccgcg gcgcggctac tcgatgccta cttcagcggg gccggtggcc
     1381 cggcgacggc tctcccgcgc gagctcgcgc attgggtcgc gggccagcgg gctgtgtccg
     1441 cccgccgttt cgagtcgccg ccgccggccg agccgctggt ccgcttgcgc gagggcaaac
     1501 gcctcgtcgt ccgatacgcg cctgcgttgc gcccgaacga gcgcgaggtc ctgctgttgg
     1561 aggagacgcc cgaagcggcg ctggccgcgt cgcccctcat ggagttgggg ctcacggaac
     1621 gggaggggca gatcgcggtc attctggctt ccggcatgac gaacgcggag atcggctcgg
     1681 ctctgggtat cagcgagaag acggtgagaa cgcacctgga gcgcatctac gagaagctgc
     1741 gcgtcgcaaa ccgggcggcg gcggtggcct tcgtctacga tgcccagcat cgccaagacg
     1801 aggcggaacc ggcggctggc cacgcgcttg ggccgcgcgc cgtgctaggc taagacgatc
     1861 gttgttcgtc gccggcagtc ccgcatgaga gggctcgccg gctcgacggt cgtgcgtagt
     1921 cagcatgtgg cgcgggggcg caggtcgtcc cgacgccgag cagaggagga tgcaggatgc
     1981 cgacgagaag tgctcaggcc gagtggcggg gagatttgca gcagggccgc gggtcgatgt
     2041 gggtcggcga ggggaccgag gtcgtctaca cgtccgactc gcgcttcgag tcgggctcgg
     2101 gcggcaaccc ggaggagctc atcgccgcgg cgcacgcggg gtgcttctcg atggcgctct
     2161 cggctgctct ggcgcgggcg ggcttcccgc ctacacgcat tcgaacgaag gcgagcgtgc
     2221 gactggagcg gttggagagc gggttcggca tcacgggaat caacctcgag actgagggcg
     2281 aggtgccggg catcgacgaa gcgacgtttc ggcgcgaggc ggagggggcg aagcagaact
     2341 gcccggtctc gaaggcgctc gccgccgtcc cggagatcac gctgacagcg cggctcgtgc
     2401 agggctaagc cggcgagcac tggggagagc cgtccgtgta gcacggcagg gtggcgggca
     2461 cgcctcgggt tggcccagcc cagatacgaa agtagtggcg cgctcggagg gattcgaacc
     2521 cccgaccctc ggttccgaag accgatgctc tatccgctga gctacgagcg ccgatccgcc
     2581 tcgatccgag tgtagcatga ccctacgccc cctcgcgctc cgagggtcgt tcgcgggctc
     2641 gcgtccctag cgcgcaggct gcaccggcaa gaccaggcgg ctcggatgcc ccgcgtcgtg
     2701 atagatgacc tgccgggcgg ggcgcacctg cgtctcttcg
//
LOCUS       DATMBB010000094         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_148277, whole genome shotgun sequence.
ACCESSION   DATMBB010000094 DATMBB010000000
VERSION     DATMBB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148277"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..524)
                     /locus_tag="VNL92_02015"
     CDS             complement(87..524)
                     /locus_tag="VNL92_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWO93517.1"
                     /translation="MIDQQLMKADWSKVKEGDEYEFQVADVTRTHFVRYAGGGGDFNP
                     IHHDQTFAEAAGLPTVFGMGMFTAGVLSRVPTEWFGATSVKRYAIRFNSRLWPGDTVT
                     FKGRVVRVYEQDGMKHADLELSATNQKGENLIAGEATVRDWRP"
     gene            complement(521..967)
                     /locus_tag="VNL92_02020"
     CDS             complement(521..967)
                     /locus_tag="VNL92_02020"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO93518.1"
                     /translation="MAYINPPGVPIERGKVHEFANAILDDNPLYHDEEAAKRAGLPSV
                     VAPPTFTSVAAFFPEKGAEGGPRMEGLDLRFVLHGGQEFNFERPLFAGDLLHAEPGET
                     RQYEKQGRRGGTMKFVETETVYRDQKGDVVLRIKNTLIQTGGVVQP"
     gene            1157..1963
                     /locus_tag="VNL92_02025"
     CDS             1157..1963
                     /locus_tag="VNL92_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO93519.1"
                     /translation="MDFEEISYDKSEHIATITLNRPERMNAFGDRMIGEWVAALEDAR
                     LDDDVRVIILTGAGRGFCAGMDVKASAEGRGLDRAATTIADRRNWLKNSVQRVPRLLT
                     QLDKPYIAAVNGPAAGAGMDMASMADIRIAAESARFSMAYVRMGLIPGDGGCYYLPRI
                     IGTAKALEYIWTGDTMSAEEALRLGYVSKVVPDDKLMEEARAFAARLASGPAVAIQLA
                     KRLVYRSWEVDANKALEMAESAMLVAQSTEDAKEGPRAFVEKRPPQFQGR"
     gene            2073..>2750
                     /locus_tag="VNL92_02030"
     CDS             2073..>2750
                     /locus_tag="VNL92_02030"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO93520.1"
                     /translation="MPLDPQAQAMIDQMQAAGAPPLHTLPPEMARQVMAMMRPPSDQI
                     EQVAKVEDRTVPTPDGDVPIRIYTPAGSAPFPVTVYLHGGGWVIGSIETHDPICRALA
                     NAAGCIVVSVDYRLAPEAKFPAAVNDSYAATKWVADHCASFGGNPSRVATAGDSAGGN
                     LAAVVALLAKERGEPKLVFQLLVYPVTEHSFETGSYKANGEGYFLTTDAMRWFWGHYL
                     NDHSDGDN"
BASE COUNT          485 a          878 c          910 g          477 t
ORIGIN      
        1 ggctcggagg gggcgagaga gcgcgacacc gtcgcgctcc cgtcagcgga ggtcgctcgg
       61 cttctcgttg agaccagcgc gccgagctac ggacgccagt cgcggaccgt ggcctcgccc
      121 gcgatgaggt tttcgccctt ctggttcgtc gccgaaagct ccaggtccgc gtgcttcatt
      181 ccgtcctgct cgtacacccg gacgacccgg cctttgaagg tgaccgtgtc gcccggccag
      241 aggcgggagt tgaagcgaat ggcataccgc ttcacggagg tggcgccgaa ccactccgtt
      301 ggcacgcggc tcagcacacc tgcggtgaac atgcccatcc cgaacacggt cggcaggccg
      361 gcggcctccg cgaacgtctg gtcgtggtgg atggggttga aatcgccgcc accgcccgcg
      421 tagcgcacga agtgcgtgcg cgtgacgtcg gcgacctgga actcgtactc gtcgccttcc
      481 ttcaccttgc tccagtcggc cttcatgagt tgctggtcga tcatggttgc accaccccgc
      541 ccgtctggat gagcgtgttc ttgatccgga gcacgacgtc gcccttctgg tcgcgataca
      601 cggtttccgt ttcgacgaac ttcatcgtgc cgccgcgccg tccctgcttc tcgtactgac
      661 gcgtctcgcc gggctcggcg tggagcaggt cgcctgcgaa gagggggcgc tcgaagttga
      721 attcctgccc gccgtggagg acgaaccgga gatcgaggcc ctccatccgt ggcccgccct
      781 cagctccctt ctcggggaag aacgccgcga ccgaggtgaa cgttggcggt gcaacgacgg
      841 atggcaaccc cgctcgcttg gccgcctcct cgtcgtggta gagggggtta tcatcaagga
      901 tggcgttcgc gaactcgtgc actttccccc gctcgatcgg gaccccgggc ggattgatgt
      961 aggccatcga cgtatcctct cactcaggtg cttcccatcg gccggcggca gcaagcgaat
     1021 gctggttcgg gccggcggtt cgagactcta tcactcagca tccccctcgt caaccgcaac
     1081 aaggcctgtt ccgaggcgct acaatacgca cgctcatctg cgggaccagg attagtgacg
     1141 aaagcgagga gccgccgtgg acttcgaaga aatctcgtac gacaagtccg agcacattgc
     1201 caccatcacc ctgaatcggc ccgaacgaat gaatgcgttc ggtgacagga tgatcgggga
     1261 gtgggtggcg gcgctcgagg atgcgcgtct cgatgacgac gttcgcgtca tcatcctgac
     1321 gggagcggga cgcggcttct gcgcgggcat ggacgtcaag gcctcggcgg agggtcgtgg
     1381 cctcgatcgg gcagcgacga cgatcgccga ccgccggaac tggctcaaga actctgtcca
     1441 gcgcgtgccc aggcttctca cgcagctcga caagccctac atcgcggccg tcaacgggcc
     1501 ggcagcgggc gcaggaatgg acatggcgag catggccgat atccgcatcg cggcggagag
     1561 cgctcgattc agcatggcct acgttcgcat gggcctgatt cccggcgatg gcggatgcta
     1621 ttacctgccg cgaatcatcg gcactgccaa ggccctcgag tacatctgga ccggcgacac
     1681 catgtcggcg gaggaggcgc ttcgtctggg gtacgtcagc aaggttgtgc ccgacgacaa
     1741 gctgatggag gaagcgcggg cgtttgcggc gcgtctcgcg agtggcccgg ctgtcgccat
     1801 ccagctcgcg aaaaggctcg tctaccgctc gtgggaggtc gatgcgaaca aggcactaga
     1861 gatggccgag tccgccatgc tggtcgccca gagcacagag gacgccaagg agggcccgcg
     1921 ggcattcgtg gagaagcgtc ccccgcagtt ccagggcaga taagcccgga gccaggccgg
     1981 cttagctctt ttgtcgaagg cgaaacgttg acgccgttcg gcctttcttg ctaaggtcgc
     2041 ggcgagcgag acgaagtagg gggaacagaa cgatgccgct cgatccacaa gctcaagcga
     2101 tgatcgatca gatgcaggcg gcgggcgctc caccgctgca caccctccct cccgagatgg
     2161 cgcgtcaggt tatggcgatg atgcgtccgc cgtccgacca gatcgaacag gtcgccaagg
     2221 tcgaggacag aacggtgccc acgccggatg gcgatgtgcc cattcgcatc tacacgccgg
     2281 cggggtcggc gcccttcccc gtgacggtgt acctgcacgg aggcggatgg gtgatcggca
     2341 gcatcgagac gcacgatccg atctgccggg cgctggcgaa cgcggctgga tgcatcgtgg
     2401 tgtcggtcga ttaccggctc gcacccgaag ccaagttccc ggcggcagtg aacgattcgt
     2461 atgcggcgac gaagtgggtc gccgaccact gcgcctcgtt cggcggcaac ccgagccgtg
     2521 tggccaccgc cggcgacagc gccggcggga acctggcggc cgtcgtggcg ctgctcgcca
     2581 aagagcgcgg cgaaccgaag ctcgtcttcc agctcctcgt atatcccgta accgagcaca
     2641 gctttgaaac cggatcgtat aaggctaacg gcgagggcta cttcctcacg accgacgcga
     2701 tgcgttggtt ctgggggcac tatttgaacg accattcgga tggcgacaac
//
LOCUS       DATMBB010000095         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_580671, whole genome shotgun sequence.
ACCESSION   DATMBB010000095 DATMBB010000000
VERSION     DATMBB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580671"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1265
                     /gene="ruvB"
                     /locus_tag="VNL92_02035"
     CDS             183..1265
                     /gene="ruvB"
                     /locus_tag="VNL92_02035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWO93521.1"
                     /translation="MPILSAEGEHMGESSRTEAARVVSGARREDDASLDVTLRPKRLD
                     EYIGQDKVKENLAIAIAAAQARGEPLDHILLYGPPGLGKTTLANVIAHELGVGLRVTS
                     GPAIERAGDLAAILTSLEPHDVLFIDEVHRLARPVEEVLYPAMEDRALDIVLGKGPAA
                     RSVRLALPPFTLIGATTRYALLSAPLRDRFGAVFRLDFYDERALATIALRSSSILEVP
                     LESDAAVLLAGRSRGTPRVVNRLLRRVRDYAQVLGDGRITAELAEDALGRLEVDHLGL
                     DEVDHKILRSIIEKFAGGPVGLETIAASISEDPGTVMDVYEPYLLQVGFIQRTPRGRV
                     ATQRAYAHLGLEREDAEPPVQPRFWE"
     gene            1340..2056
                     /locus_tag="VNL92_02040"
     CDS             1340..2056
                     /locus_tag="VNL92_02040"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HWO93522.1"
                     /translation="MTIVTVSGNVGTGARDVARMIADALGLDYVDQALLVDAARRLGV
                     PVERVSQRDERTQSLGERMAAALRSFLERSATIGAADPMIGAPSIEVMLGRPYEEEIA
                     EATESDVSDKRYGEALAAIIQEAASRGDVVIVGRGSHAVLQGHPVALHVNLVAPRDAR
                     VRNLVRREGLDPEDASRRVERSERGRIAYHRKLFKCEPDDPLMYHMTLNAALLDVETM
                     SRSILACLADDASSLRGGTP"
     gene            2053..>2483
                     /locus_tag="VNL92_02045"
     CDS             2053..>2483
                     /locus_tag="VNL92_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWO93523.1"
                     /translation="MTDRREPIASRPHMPGYGIAEDTSGLLPWSWAEERLTACRNYWI
                     ITVNADGSPHAMPVWGVWQDGGFLFGTGERSRKARNIARDPRIVVATEGGDEPIVLHG
                     VAVRVTESDTMAPFLEAYERKYDWGAGESSDDTNSDNDPMFL"
BASE COUNT          422 a          847 c          830 g          384 t
ORIGIN      
        1 ctcgacgaat cgcgagatgg ggtacgcgaa gatcgtgccg aagccgtcga aggccagcgc
       61 tcgcacggcg ccgggttcga agggggaagt gacgtcgcgg ctcacgccgt cactgtacgc
      121 tatgctcggg gcgggcgtaa gtgcctcacc ctggagtctg gtccttcctg gaagcatgaa
      181 acatgcctat actgagcgcg gagggcgaac acatgggcga atcgtctcgg accgaggccg
      241 cccgcgtcgt ctccggcgcg cgacgtgagg acgatgcgtc gctcgacgtc acgttgcggc
      301 cgaagcgcct cgacgagtac atcggtcagg acaaggtgaa ggagaatctg gccatcgcca
      361 tcgctgcggc gcaggcccgt ggcgagccgc tcgaccacat ccttctgtat ggtccgcccg
      421 gcctcggcaa gacgacgctc gccaacgtca tcgctcacga gctcggcgtc ggcctgcgcg
      481 tcacctcggg ccccgccatc gaacgggcgg gggacttggc cgccatcctg acttcgctcg
      541 agccccacga cgtgctgttc atcgacgagg tgcaccgcct ggcccgtccc gtcgaggaag
      601 tgctctaccc ggcgatggag gaccgcgccc tcgatatcgt cctggggaag gggccggccg
      661 cgcgcagcgt tcgtctcgcc cttccgccgt tcaccctcat cggcgccacg acccgttacg
      721 ccctgctctc ggcgcccctt cgcgaccggt tcggcgccgt gttccgcctc gacttctacg
      781 acgagcgagc gctggccacg attgccttgc gcagcagcag catcctcgaa gttcccctgg
      841 aaagcgacgc tgccgtcctg ctggcaggcc gctcccgtgg gacaccgcgc gtcgtcaatc
      901 gcctgcttcg gcgcgtccgc gactatgcgc aggttctcgg cgatgggcgc atcactgccg
      961 agctcgccga ggacgcgctg ggccgcctgg aggtcgacca tctcgggctc gacgaggtcg
     1021 accacaagat cttgcggtcc atcatcgaga agttcgccgg cggaccagtc ggcctggaaa
     1081 ctatcgccgc ctccatctcc gaggacccgg gaacggtgat ggacgtctac gagccgtatc
     1141 tgctgcaagt cggcttcatt caacgaaccc cgcgcggccg cgtcgccacc caacgcgcgt
     1201 acgcccacct tggcctggaa cgtgaggacg cggagccacc ggtacagcca cgcttctggg
     1261 agtaggaccg gggtcacagc agcggcgtgc tgtacgctac cctatacgga acgccgcctt
     1321 ggggaaggga ggccgcgcag tgaccatcgt aacggtatcg ggcaacgtcg ggaccggcgc
     1381 gcgcgacgtc gctcggatga tagctgatgc gctcggcctc gattacgtcg atcaggcgct
     1441 gctcgtcgac gccgcacggc ggctgggcgt gccggtggag cgggtgagcc agcgcgacga
     1501 gcggacccag tcgctcgggg aacggatggc cgctgccctg cgctcgtttc tcgagcgatc
     1561 ggccaccatc ggcgcggcag atccgatgat cggcgcgccg agcatcgaag tcatgctcgg
     1621 ccgcccttac gaggaagaga tcgcggaggc tacggaatcc gacgtcagcg acaagcgcta
     1681 cggcgaagcg ctggcggcga tcatccagga ggctgcgagc cgcggcgatg tggtcatcgt
     1741 cggccgaggc agccatgccg tgctgcaggg acacccggtg gcgctgcacg tcaatctcgt
     1801 ggcgccgcga gacgcccgcg tgcggaacct cgtccggcgc gaggggctcg acccagagga
     1861 cgcgagccgg cgcgttgagc gcagcgagcg tggacgcatc gcgtaccatc gcaagctgtt
     1921 caaatgcgag ccggatgacc cgctcatgta ccacatgacg ctcaacgccg cgcttctcga
     1981 tgtcgagacc atgtcgcgca gcatcctcgc gtgtctcgcc gacgacgcaa gtagcctacg
     2041 cggaggaacg ccatgaccga tcgccgagaa cccatcgcga gccgccccca tatgccgggt
     2101 tacggcatcg cagaagacac gagtggcctc ttgccgtgga gctgggccga ggaacggctc
     2161 acggcatgcc ggaactactg gatcatcacc gtcaacgccg acggctcgcc gcacgcgatg
     2221 cccgtttggg gcgtatggca ggacggtggc ttcctgttcg gtaccggcga gcgttcccgg
     2281 aaagcacgca atattgcgcg cgatccccgc atcgtcgtgg ccacggaagg cggcgacgag
     2341 cccatcgtac ttcacggcgt ggcggtccgc gtcaccgagt ccgacacgat ggcgcccttc
     2401 ctcgaagcgt acgagcgcaa gtacgattgg ggcgcgggcg agagcagcga cgacacgaac
     2461 tccgacaacg accccatgtt cct
//
LOCUS       DATMBB010000096         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_21044, whole genome shotgun sequence.
ACCESSION   DATMBB010000096 DATMBB010000000
VERSION     DATMBB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21044"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VNL92_02050"
     CDS             <1..377
                     /locus_tag="VNL92_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWO93524.1"
                     /translation="PVLLFVAVTVWVAGFDLLYALQDVEFDRSTGLHSVPADLGIPAT
                     LWIARGMHVAMIAFLAWAGAAESLGVLYWIGLAVASALLIYEHQLVRPNDLSRLDVAF
                     FNMNGYIAVTVFAFTLGGLYVG"
     gene            367..957
                     /locus_tag="VNL92_02055"
     CDS             367..957
                     /locus_tag="VNL92_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWO93525.1"
                     /translation="MSASPPLVVGVTGASGAELGRRLMLRLAEARIPLVVTATEAGRR
                     VWTEELGTPLKATLAELGEAVRYADIRDIGDAIASGSVPTRGMVVVPASMNLVAAVAA
                     GRAENLLERAADVTLKEGRQLTLVPRETPLSAIHLRNLLTLARLGVRIVPPMPAFYLR
                     PQSLDDLYAALTERIIQTLALPDLPPAEDWQYRSDA"
     gene            869..1393
                     /locus_tag="VNL92_02060"
     CDS             869..1393
                     /locus_tag="VNL92_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWO93526.1"
                     /translation="MLRSRNASSRPWPCRTFLRRRTGSIDRTPRHSGIACAPLGNETG
                     EWNAGHAPRSIPHGLPRDEQAGRGSAFYRDALGLTLSEESEFALVFDASGTMLRVQKV
                     EQFTPQPFTVLGWQVDDVERIVSILVERGIQPERYPGLGQDRFGAWISPDGARTVWFR
                     DPDGNILSLTQFPA"
     gene            1506..2171
                     /locus_tag="VNL92_02065"
     CDS             1506..2171
                     /locus_tag="VNL92_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO93527.1"
                     /translation="MDWPGGSWEATAVIVGIIVGAYLAVLWLSLVLWTYRDISRRTRD
                     PALQVGAVLLVLAFNILGLFLYLILRPGETLTEAYERALEEEALLAELQEQAGCPECR
                     RPVAEDYLICPHCTARLREQCRHCQRLLSRSWVACPYCGRERVESRERRPLQRTAARG
                     ASEDARRPAEPVSASDRPLSALSSELESRLSATEVRPAASPGGPRPGLRDANDEARRT
                     GTD"
BASE COUNT          367 a          770 c          758 g          411 t
ORIGIN      
        1 cgcccgtgct gttgttcgtc gccgtcacgg tctgggtggc cggcttcgac ctcctctacg
       61 ccctgcagga cgtcgaattc gatcgatcga cagggctgca ttctgtcccg gcggacctcg
      121 gcatcccggc gacgctctgg atcgcgcgcg gcatgcacgt ggccatgatc gcgtttctcg
      181 cctgggccgg ggcagcggaa agcctaggcg tcctgtactg gattggcctc gccgtggcgt
      241 cggcgcttct catctacgag catcagctcg tccgccccaa cgacctctct cggctggacg
      301 tggccttctt caacatgaac ggttatatcg ccgtgacggt gttcgcgttc acgctcgggg
      361 gattgtatgt cggctagtcc cccgctcgtc gtcggcgtca ccggcgcgag cggggcggag
      421 ctcgggcgcc ggctgatgct ccgcctcgcg gaggcacgga tcccactcgt cgtcacggcg
      481 acagaggccg gcaggcgcgt gtggacggag gagctgggga ctccgctgaa ggcaacactg
      541 gcggagttgg gcgaagcggt tcggtacgcc gacatccggg acatcggcga cgccatcgcg
      601 agcggcagcg tgccgacacg ggggatggtc gtggtgcccg ccagcatgaa cctcgtcgca
      661 gccgtggcgg cggggcgtgc ggagaatctt ctggagcgtg ccgccgacgt cacgttgaag
      721 gagggccgcc agcttacgct ggttccgcgc gagacaccgc tctccgcgat ccaccttcgg
      781 aacctgctga cgctggcacg gctcggcgtc cgcattgtcc cgccgatgcc cgccttctac
      841 ctgcgtcccc agtcgctcga tgacctctat gctgcgctca cggaacgcat catccagacc
      901 ctggccctgc cggaccttcc tccggcggag gactggcagt atcgatcgga cgcctaggca
      961 cagcgggatc gcatgcgccc ctctcggcaa cgagacagga gaatggaatg ccggtcacgc
     1021 tccgagaagc atccctcatg gccttcctcg cgacgagcag gccggacgag gctctgcatt
     1081 ctatcgagac gccctcgggc tcaccctctc ggaggagagc gaattcgcgc tcgtgttcga
     1141 tgccagtggg acgatgctgc gagtccagaa ggtcgagcag ttcacgccgc aaccgttcac
     1201 ggtgctcggg tggcaagtcg atgacgtcga acgaatcgtg tcgattctag tggaacgcgg
     1261 cattcaaccc gagcgctacc ctggactcgg gcaagatcga ttcggcgctt ggatttcgcc
     1321 ggacggcgcc aggactgtgt ggttccgcga ccctgacggg aacattctct cgttgacgca
     1381 gttcccggca tgaactcgca acatcgatgg gctgccaaag aatggcctat acggggaacc
     1441 ttgctatact tctccacaat gagcgcccga acgcagatgg gcgcgcaagc ggaatcgagg
     1501 actgaatgga ttggcccgga ggaagctggg aagccacggc cgttatcgtc ggcatcatcg
     1561 tcggcgccta cctcgcggtt ctttggctca gcttggtact gtggacgtat cgcgatatca
     1621 gccgccgcac gcgcgatcct gccctgcagg tgggcgccgt gcttcttgtg ctggcgttca
     1681 acatcctcgg gctgtttctc tacctcatcc tccgccccgg tgaaacattg acggaggcgt
     1741 atgagcgcgc tctcgaagag gaggccctcc tcgctgagct tcaagagcag gccggctgcc
     1801 cggaatgccg ccgccccgtc gccgaagact acctgatttg cccgcactgc acggctcgcc
     1861 tacgcgagca gtgccgccac tgccagcgcc tgctgagtcg cagttgggtc gcatgtcctt
     1921 actgtggccg cgagcgcgtg gagtcgcgcg aacgccgccc gctccagcgg acagcggcga
     1981 gaggcgcatc agaagatgcg cggcgccccg ctgagcccgt ctccgcctcc gaccgtccgc
     2041 tgagcgcctt gagcagcgag ctcgagtcgc gcctctcagc gactgaagtg cggcccgctg
     2101 caagccccgg tgggccaagg cccggactgc gcgatgcgaa cgacgaggca cggcgcaccg
     2161 ggacggactg accgcgccat gcctgtcgag gtgcgtcccg tggctgacgg cgatcgcgaa
     2221 tgggtcgcct cgcttgtcgc cagcacgaac ggctccgcgc gcgtggtctc gcgcggacga
     2281 ttacacgatc caacgtcgct gccagg
//
LOCUS       DATMBB010000097         3148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_461180, whole genome shotgun sequence.
ACCESSION   DATMBB010000097 DATMBB010000000
VERSION     DATMBB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461180"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..267)
                     /locus_tag="VNL92_02070"
     tRNA            complement(193..267)
                     /locus_tag="VNL92_02070"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(233..235),aa:Lys,seq:ttt)
     gene            357..1256
                     /locus_tag="VNL92_02075"
     CDS             357..1256
                     /locus_tag="VNL92_02075"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWO93528.1"
                     /translation="MPPKYLAIVSVVGRDQKGIVARVSTYLAEHEVNIEDIEQRVIHG
                     QFMMDMLVDLAGLRGDLDSLIEGLNTIGRDLGMSIRVMLHRAGRRRRVAVLVSKEPHC
                     LARLIDDAEHGALRGDIVVVLSNHEVLRPMAEEHGIPFFACPAEDMPAHEDFLLEKLR
                     EHQADLVVLARYMRILSARVVEPYAERMINIHPSLLPYFPGPNPYRRAWEDGVRVTGC
                     TAHFVTQDLDAGPIILQDVFQIDVGEDSEADVRTKGRALEAEVLSRAVQLYLNDEIVM
                     LEGKVVFKPGMRTFLKDLERTAD"
     gene            1315..1860
                     /locus_tag="VNL92_02080"
     CDS             1315..1860
                     /locus_tag="VNL92_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93529.1"
                     /translation="MTLEVVVIAAIRILGSLPVLRWAFAGALIAIGVDLGDLFLREWL
                     DLGGIPDYQRFDKYADIAYMLTFLAVALRWPAQPRRIAVGLFGMRTIGFVAFEATGQR
                     EILLVFPNVFEFWFVFVAGQRHWLPRFEFTAARVSAVLAGLTALKLFQEYALHWGKWL
                     DGFTAFEAVDAVRDWVVSFAP"
     gene            complement(1873..2490)
                     /gene="lepB"
                     /locus_tag="VNL92_02085"
     CDS             complement(1873..2490)
                     /gene="lepB"
                     /locus_tag="VNL92_02085"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWO93530.1"
                     /translation="MSFSRFMRGAGRDLIETAILALLIFFAVRSAVQNFKVEGFSMDP
                     SLENGEYILVDKVSYGVFDLGPLERFIPFVDSGDDGFLFEGPQRGDVIVFEAPIAEGR
                     DFIKRVIGVPGDQIEVRDGVVYVNGQAIDEPYITHKGSYDWPADGSGPATVPDKHYFV
                     LGDNRDNSSDSHIWGFLPADKVVGRAWVSYWPTSSIGLAPHHAIP"
     gene            2593..2799
                     /locus_tag="VNL92_02090"
     CDS             2593..2799
                     /locus_tag="VNL92_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93531.1"
                     /translation="MTKQVLGMSADELHAVARLGTADSGDEPRALHVQRAEAMLRALV
                     AVMDANNRRLTDDVRAFGLGLSGF"
     gene            2810..3022
                     /locus_tag="VNL92_02095"
     CDS             2810..3022
                     /locus_tag="VNL92_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93532.1"
                     /translation="MNFIDFGLSEAQLQALVESEAEAALGPDWRTAPAHDATAAALLA
                     ILIKVVCENNRRLTEQFESLGLRLRS"
BASE COUNT          533 a         1066 c         1015 g          534 t
ORIGIN      
        1 cgatggccgg ggattcctgc tcgggccatg gttcgaagtt acatcctgag gcggccaggc
       61 ccgttcgatt ccaggccctt atagcccgcc aacgggccgc ctccgagaac cgtcgccgca
      121 gtcgttgcca tcaattgctg agaagacatg ctagatggcc tccctgacga agccaacagg
      181 cgcgattccc cgtggtgagc cgggtgggat tcgaacccac gacccacggg ttaaaagccc
      241 gctgctctgc cactgagcta ccggcccacc ctcagtgtag cagcactccc cgaagccacg
      301 tcgcgcacgg cctcccggcg ctcgcgcatc cgcgactccc cctgtatgct cgtgaggtgc
      361 cgcccaagta cctcgccatc gtcagcgtcg tcggccgcga ccagaagggc atcgtggccc
      421 gcgtctccac gtacctcgcc gagcacgagg tcaacatcga ggacatcgag cagcgggtca
      481 tccacggcca gttcatgatg gacatgctcg tcgacctcgc tggcctgcgc ggcgacctcg
      541 atagcctcat cgagggcctc aacaccatcg gtcgggacct cggcatgagc atccgcgtca
      601 tgctgcaccg cgcgggccgc cggcgacgcg tcgccgtcct cgtcagcaag gaaccgcact
      661 gcttggcccg cctgatcgac gatgccgagc acggcgccct gcgcggcgat atcgtcgtcg
      721 tgctcagcaa ccacgaggtg ctccgcccga tggccgagga gcacggtatc cccttcttcg
      781 cctgcccggc cgaggacatg cccgcgcacg aggacttcct cctcgagaag ctgcgcgagc
      841 atcaggccga cctcgtggtt ctggcgcgct acatgcgcat cctcagcgcg agagtcgtgg
      901 agccctacgc ggaacgcatg atcaacatcc acccctcgct gctgccctac ttccccgggc
      961 cgaaccccta ccgtcgcgcg tgggaggacg gcgtgcgggt cacgggctgc acggcccact
     1021 tcgtgaccca ggacctcgac gcgggcccga tcatcctcca ggacgtgttc cagatcgacg
     1081 tgggggagga cagcgaggcg gacgtgcgga ccaagggccg cgcgctcgag gcggaagtgc
     1141 tgagccgcgc cgtccagctc tacctcaacg acgagatcgt catgctggag ggcaaggtcg
     1201 tgttcaagcc cggcatgcgc accttcctca aggacctcga gcgaaccgcg gactagcccg
     1261 ccccagaggg cgccgacagc ggatggttcc gcttcctagc ccgaggagcg tccgatgacg
     1321 ttggaggtcg tcgtcatcgc agcgatccgc atcctgggat cgctgccggt gctgcgatgg
     1381 gcgttcgccg gcgccctcat cgccattggg gtggacctcg gcgacctgtt cctacgggag
     1441 tggctcgacc tcggcggcat ccccgactac cagcgattcg acaagtacgc cgacatcgcc
     1501 tacatgctca cgttcctcgc tgtggcgctc aggtggccgg cgcagccgcg gcggatagcc
     1561 gtcgggctct tcggtatgcg gaccatcggc tttgtcgctt tcgaggcgac cgggcagcgc
     1621 gagatactgc tcgtgttccc gaacgtcttc gagttctggt tcgtgttcgt cgccgggcag
     1681 cggcactggc tgccgcgatt cgagttcacg gcggcgcgag tatcggccgt gctcgccgga
     1741 ctcacggcgc tgaagctctt ccaggagtac gcactgcatt ggggcaagtg gctcgatggc
     1801 tttaccgcct tcgaagcggt ggacgccgtg cgcgattggg tcgtatcgtt tgcgccctga
     1861 ggccgagcgg gctcagggga tggcgtggtg cggcgccagg ccgatggacg acgtaggcca
     1921 gtacgagacc catgctcgcc cgacgacctt gtcggcagga aggaatcccc agatgtgcga
     1981 atcgctgctg ttgtcgcggt tgtcgcccag aacgaagtag tgcttgtccg gcaccgtggc
     2041 cggcccgctg ccatcggccg gccagtcgta cgaccccttg tgcgtgatgt acggctcgtc
     2101 gatggcctgc ccgttcacgt agacgacgcc gtcgcgcacc tcgatctggt cgccggggac
     2161 gccgatgacc cgcttgatga agtcgcggcc ctcggcgatg ggcgcctcga agacgatgac
     2221 atcgcctcgt tgcggtccct cgaagagaaa gccatcgtca cccgaatcca cgaaggggat
     2281 gaagcgctcc agcggcccga ggtcgaacac gccgtagctc accttgtcga cgaggatgta
     2341 ctcgccgttc tcgaggctcg ggtccatgct gaacccttcg accttgaagt tctgcacggc
     2401 cgaccgtacg gcgaagaaga tgaggagcgc gaggatcgcc gtctcgatga ggtcgcggcc
     2461 cgcgccgcgc atgaagcgag agaatgacaa ggagacgcgc cctcggatag ctgcaccggt
     2521 atgcccggcg ctgctcgtgc tatggtgcct atcagtatag cacctgaatc tcgtcagccg
     2581 gagagttcga ccatgacaaa gcaagttctg gggatgagcg cagacgagct gcatgctgtt
     2641 gcgcggctag gcacggcgga ctcaggagat gagccgcggg cattgcacgt gcaacgagcc
     2701 gaagcgatgt tgcgcgccct cgtcgccgta atggacgcga acaaccgccg cctaacggac
     2761 gacgttcggg cattcggcct cggcctgtcg gggttctgaa gccatggcga tgaacttcat
     2821 cgacttcggc ctgagcgaag cccagctcca ggcgctcgtc gaatcggagg ccgaggccgc
     2881 cctgggcccg gattggcgaa cggctcctgc ccacgacgcg accgcggcgg cgctcctcgc
     2941 catcctgatc aaggtggtgt gcgagaataa ccgccgtctc accgagcagt tcgaatcgct
     3001 gggcctccgc ctccggtcct agcgcgcgat gtatgcttcg cgaaccgaga cgacgaaggg
     3061 caacagtcta cttcccaccc ctccgcagca gctcagcgag gagcgttcgg tggcagcgtc
     3121 cgccgtcccg gcacacgcac atcacggt
//
LOCUS       DATMBB010000098         4894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_143828, whole genome shotgun sequence.
ACCESSION   DATMBB010000098 DATMBB010000000
VERSION     DATMBB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143828"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /gene="murD"
                     /locus_tag="VNL92_02100"
     CDS             <1..1461
                     /gene="murD"
                     /locus_tag="VNL92_02100"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWO93533.1"
                     /translation="SRDAVLAHWHRRGDGRCGDGVAGDVMDIDLRSQRVLVVGIGIEG
                     VDLVRFAVAEHAARVTANDARGREDLVRRIEDLAGLPVEFALGGHDPALARDHDVVLV
                     SQGVRLDLPLIAEARRQGKPVASLTSIFLSRCRGPVLGVTGSSGKTTTTALLGAMFRE
                     AGLPHVVGGNIGVPLLSRLAEIDERTWVVLEVSHTQLELTDRSPHVAAVTNVTPNHLD
                     RYTWDAYVRLKRNFIIYQRPEDFAILNYDDPVTRSFADDTTAKVNFTTMGPTAPGDGA
                     YLPNGVLTIRRGLREVPVLPAADIPLRGTHNVANSLTAIAAADAAGVPVDAMARAIRS
                     FPGVPHRLEPIGTIRGVTWVNDSIATTPERTLAGMRSFTEPLVVLLGGRGKNLPFEEL
                     ASEAASRCRAVVCFGEEREELAQAMREAGVRTLVVENIDEAVNAAADIAEPGDVVLFS
                     PACTSFDAFDNFEQRGEAFRACVRMLAERWGEEVPS"
     gene            1437..2705
                     /gene="ftsW"
                     /locus_tag="VNL92_02105"
     CDS             1437..2705
                     /gene="ftsW"
                     /locus_tag="VNL92_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWO93534.1"
                     /translation="MGRGGALIAASVEIGRERIRAAQPDYLVLVGVAVLTVFGVLIVY
                     SASFAFGTLEFNDGNYFVVRQAAYAVVGGVGMLLAMRMDYRHLRKFSLVLLVASMVLL
                     LATHVPGLGVESNGARRWISVGPLPPLQPSEFVKLALIIYLADWLTSRGDDVRSFTLG
                     VAPFTFVVGPVAALVMTQPDMGTMLILLAITGTQLFVGGASVRHVIALAGATIFGALL
                     LITSGGYRTERLFAFLSPEEDAAGSGFHILQLLIALGSGGLTGVDWGESRQKFFYVPG
                     AHTDGVFAILGEELGFLGGMAVIVIFATVIFRGLRIADRARDPFGHLLAVGIVSWFGY
                     QMLLNIGGITRSIPMTGVPLPFLSYGGSALIAAMTGAGLLLSVSRFGHNAPQRATSQT
                     PKRGAASRAAGSRATSKRRASSAAWRPRTG"
     gene            2705..3805
                     /locus_tag="VNL92_02110"
     CDS             2705..3805
                     /locus_tag="VNL92_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HWO93535.1"
                     /translation="MRIVLTGGGTAGHVFPALSVATALREECGRLGLPLDLLYLGLRT
                     GPEARIAPQHGVPFAAVSARPLRGRNPIAMLRALFSIAWGTLRARSLLRGSAGVLATG
                     GYVTVPVALAARSRRTPLVVYLPDVEPGWAVRLTSRLARRIATTTDRGLAHLPRGKAE
                     ATGYPVRPEFRKDSRDVARSMLGIEDGERVLLVAGATQGARSLNEAVAARLEDYLRLA
                     RVIHVCGPAHEEAFKAQRDRLPIELRGRYTVDGFRDDMPRLMIAADLAVLRAGASVLG
                     ELPAARLPAILVPGPFAGAHQAHNARYLADAGAAAMLDDDELVRLFGLVESLLTDDER
                     RAAMRVRLAQLDRPNAARDIACIVLQEAGEEP"
     gene            3802..>4894
                     /gene="murC"
                     /locus_tag="VNL92_02115"
     CDS             3802..>4894
                     /gene="murC"
                     /locus_tag="VNL92_02115"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWO93536.1"
                     /translation="MTLMTDEQARTGANIPQRVHLIGIGGAHMSAIARILLARGYDVS
                     GSDLRASPTLDELSRAGARVWIGGHDASHVRNAELVVATAAATSANPEIVAAQELGLT
                     VLSRAQMVARLIAGKRTLAVAGTHGKTTTTSLAIAVVREAGLDPTFLVGGDVVGLETN
                     AAHGDGPIAIIEADEYAHAFLEYAPEIAIVTNVEPDHIDYFGSFDEIRRAFHDFPTRV
                     QRDGRAIVCADSPHLLEIMEDLRRTGEPGTTFETYTLGRERQAEWRAVDIERDEDGQS
                     FTVERAGEAFLRARITLPGRHAVANALAVVAATHALGIDSASIERGLLAARGAHRRFD
                     RHGQVCGIAVYDDYAHHPTEIRATLHAARE"
BASE COUNT          758 a         1698 c         1615 g          823 t
ORIGIN      
        1 agtcgtgatg cggttctggc tcattggcat cgccggggcg atggtcggtg tggcgatggc
       61 gttgcaggtg acgtgatgga catcgacctg cgaagccagc gagttctcgt ggtcggcatc
      121 gggatcgagg gagtcgatct cgtgcggttc gccgtggccg agcatgcggc gcgcgtaacc
      181 gcgaacgacg cacgagggcg ggaggacttg gttcggcgaa tcgaggatct agcgggcttg
      241 ccggtcgagt tcgctctcgg cggccacgat cccgctctag cccgcgacca cgatgtcgtc
      301 ctggtttccc aaggcgtgcg gctcgatctt ccgctcatcg ccgaggcccg ccgccagggc
      361 aagccggtcg cttccctcac gagcatcttc ctctcccggt gccgcgggcc ggtgctcggc
      421 gtcaccggct cgagcgggaa gacgacaacg accgcgctcc tcggcgcgat gtttcgcgag
      481 gcagggctcc cgcacgtcgt gggagggaac atcggcgtgc cgctgctctc tcgtctcgcc
      541 gagatcgacg agaggacgtg ggtcgtcctc gaagtgagcc acacgcagct cgagctcacc
      601 gatcgaagtc cgcacgttgc cgcagtcacg aacgttactc ccaaccacct cgaccgctac
      661 acatgggacg cctacgtgcg gctcaagcgg aacttcatca tctatcaacg gcccgaggac
      721 ttcgcgatcc tcaactacga cgaccccgtg acgcgatcct tcgcggacga cacgacggcg
      781 aaggtcaatt tcacgaccat gggtccgacc gccccgggag atggcgccta tctcccgaac
      841 ggcgtcctca ccatcaggcg agggctccgc gaggttccag tgcttccggc cgcggacata
      901 cccctgcgcg gaacgcacaa cgtcgcgaac tcgctgaccg cgatcgccgc cgccgatgcc
      961 gccggcgtcc ctgtcgacgc catggcccga gccatccgtt ccttcccggg cgtaccacac
     1021 cggttggaac cgatcggaac catccgtggg gtgacttggg tgaacgacag catcgccacg
     1081 acaccggagc ggacgctcgc cgggatgcgc tcgttcaccg agcctctggt cgtgcttctg
     1141 ggcggtcgcg gcaagaacct gccgttcgaa gagctcgcga gcgaggctgc atcccgttgt
     1201 cgtgccgttg tgtgcttcgg ggaggagcgc gaggaactcg cccaagcgat gcgcgaagcg
     1261 ggcgtgcgga cgctcgtcgt cgagaacatc gacgaggcgg tgaatgccgc cgccgacatc
     1321 gccgagccgg gcgatgtggt gctcttctcg ccggcttgca caagcttcga tgccttcgac
     1381 aacttcgagc agcgcggcga ggcatttcga gcctgcgtcc ggatgctggc agagcgttgg
     1441 ggcgaggagg tgccctcata gccgcgagcg tcgagatagg ccgcgagcgc atccgcgcag
     1501 cccagccgga ttacttggta ctggtgggcg tggccgtgct caccgtgttt ggggtcctca
     1561 tcgtctacag cgcaagcttc gcgttcggga cgctcgaatt caacgatggg aactacttcg
     1621 tcgtccggca ggcggcctat gccgtcgtgg gtggggttgg catgctcctc gcgatgcgca
     1681 tggattaccg ccacctccgg aagttctccc tcgtcctgct cgtggcgtcc atggtcctct
     1741 tgctggcgac gcacgtcccg ggcctcgggg tcgaatcgaa cggagcgcga cgctggatat
     1801 cggtcggccc gctaccgcct ctgcagccca gtgagttcgt taagctggcc ctcatcatct
     1861 acctcgccga ttggctcaca tcgaggggcg atgacgtccg gagcttcacg ctcggggtcg
     1921 cgccgttcac cttcgtcgtg ggcccggtgg ccgctctcgt catgacccag cccgacatgg
     1981 gaacgatgct catcctgctc gccatcaccg gcacgcagtt attcgtcgga ggggcttcgg
     2041 ttcggcacgt gatcgcgctc gccggggcga ccatcttcgg ggctctcttg ctgatcacga
     2101 gcggcgggta ccggacggag cgactcttcg ccttcctctc accggaggaa gacgccgccg
     2161 gctccggctt tcacatcctg caactcctca tcgccctcgg cagcgggggg ctcacgggag
     2221 tcgattgggg tgaatcgcga cagaagttct tctatgtgcc cggggcgcac accgatggtg
     2281 tgttcgccat tctcggggag gaattggggt tcctcggcgg catggccgtc atcgtcatat
     2341 tcgccaccgt gatcttccga gggctccgca tcgccgaccg ggctcgcgat ccgttcggac
     2401 acctgctggc agtggggatc gtctcctggt ttggctatca gatgctcctg aacatcggcg
     2461 gcatcacccg ctcgatcccg atgaccggcg tgccgctgcc gttcttgagc tacggagggt
     2521 cggcactgat cgcggcgatg accggggcgg gactgctgct ctcggtctct cggttcggcc
     2581 acaacgcacc gcagcgcgcg acgtcgcaga cacccaagcg cggcgccgca tcacgtgccg
     2641 ccgggagccg tgcgacgtcg aagcgccggg ctagctccgc tgcctggcga ccgaggactg
     2701 ggtgatgcga atcgtgctta cgggaggagg aacggcaggc cacgtgtttc ccgcactgag
     2761 cgtcgcaacg gcgctacggg aggaatgcgg caggctcggc cttccgctgg atctgctgta
     2821 cctcggcttg cggacaggac ctgaggcgcg cattgccccg cagcacggcg tgccgtttgc
     2881 cgcggtgagc gctcggccgc tacggggccg caaccccatc gcgatgttgc gcgcactctt
     2941 cagcatcgcc tggggcacac tgcgggcgag gagcttgctc cgcgggtcgg ccggcgttct
     3001 cgcaacaggc ggctacgtca ccgtgccggt tgccttggca gcgcgcagtc gccgcacacc
     3061 gctggtcgtg tacttgcccg atgtcgagcc gggctgggcg gtgcgcctaa cgtcgcgcct
     3121 ggcacggcgc atcgcaacga cgaccgaccg cgggctcgcc caccttcctc gaggcaaggc
     3181 ggaggcgaca ggctaccccg tgcgcccgga gttccgcaaa gacagccgcg acgtcgcgcg
     3241 cagcatgctc ggaatcgagg acggggagcg cgtcctcctc gtcgccggag cgacgcaggg
     3301 agcccgcagc ctcaacgagg ccgtcgctgc ccggctcgag gactatctcc ggctcgcgcg
     3361 ggtcatccat gtttgcggac cggcgcacga ggaggcgttc aaggcccagc gcgaccggct
     3421 tcccatcgag ctacgtggcc gctacaccgt ggatggcttt cgtgatgaca tgccacgcct
     3481 catgattgcg gccgacctcg cggtcctccg cgccggcgcg agcgtgctgg gcgagctccc
     3541 cgctgcgcgg ctcccggcga tcctcgtgcc cgggcccttc gccggagccc accaggctca
     3601 caacgcccgc tacctcgccg atgcaggcgc tgccgcgatg ctcgacgacg acgaactcgt
     3661 gcgtctgttt ggcctcgtcg aatcgctcct cactgacgac gagcgacggg ccgcgatgcg
     3721 tgtgcggctg gcccagctcg accgaccgaa cgccgctcga gacattgcgt gcatcgtgct
     3781 tcaggaagca ggggaggagc catgacgctc atgacggacg aacaggcccg caccggcgcg
     3841 aatattcccc agcgagtcca cctcatcggc atcggcggcg cccacatgtc ggccatcgcc
     3901 cgcatcctcc tcgcccgcgg atacgatgtc tccggctccg acctgcgcgc aagtccaacc
     3961 ctcgacgagc tctctcgcgc cggggcgcgg gtctggattg gcggccacga cgcttcgcac
     4021 gtccgcaacg cggagctcgt tgtcgccacc gcggcagcga cgagcgcgaa tcccgagatc
     4081 gtcgccgcac aggagctcgg cctcacggtg ctttcccgag cccagatggt cgcgcggctc
     4141 atcgcgggca agcggaccct ggccgtggcc ggcacgcacg gcaagaccac gaccacatcc
     4201 ctcgccatcg ccgtcgtgcg cgaggcaggc ctcgacccca cgttcctcgt gggcggcgac
     4261 gtcgtcggcc tggagacgaa cgcggcgcat ggagatgggc ccatcgccat catcgaggcc
     4321 gacgagtacg cccacgcctt tctggagtac gcgccggaga tcgccatcgt cacgaacgtc
     4381 gaacccgacc acatcgatta cttcggctcc ttcgacgaga tacgtcgggc cttccacgac
     4441 ttcccgacgc gcgtgcaacg cgacggccgg gccatcgtct gcgccgacag tcctcacctg
     4501 ctcgaaatca tggaggacct gcgaaggacc ggcgagccag ggaccacttt cgaaacctac
     4561 acgctcggac gcgaacgcca ggccgagtgg cgcgcggtcg atatcgaacg ggatgaagat
     4621 ggccagagct tcacggtgga acgcgccggg gaagccttcc tgcgggcccg catcaccctg
     4681 ccgggccgcc acgctgtagc caacgcgctc gccgtcgtgg ccgcgacgca cgccctcgga
     4741 atcgattcgg cctcaatcga gcgcgggctc ctcgccgcgc gcggcgccca tcggcggttc
     4801 gatcgacatg gccaggtgtg cgggattgcc gtctacgacg actacgctca ccacccaacc
     4861 gagattcgcg cgacccttca tgccgcgagg gagc
//
LOCUS       DATMBB010000099         4726 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_104254, whole genome shotgun sequence.
ACCESSION   DATMBB010000099 DATMBB010000000
VERSION     DATMBB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104254"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VNL92_02120"
     CDS             complement(<1..401)
                     /locus_tag="VNL92_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWO93537.1"
                     /translation="MSRRPLEGIRVVLGVSGSIAAYKAADIASRLVQEGAMVDVLLTE
                     EATKFIAPLTFRSLTNRPVPVDMFDVNSEVAEAHVELARAADALVVAPATASMLARLA
                     HGLADDMVSLTAAATRAPLLLAPAMDSLMWE"
     gene            complement(398..1186)
                     /locus_tag="VNL92_02125"
     CDS             complement(398..1186)
                     /locus_tag="VNL92_02125"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWO93538.1"
                     /translation="MLLALDIGNTVTTIGVFEGDELRANWRISTDVRKLRDEYAMLLF
                     NLLWTGKIDPADVSKCSIASVVPTLTPVFEDLARRYFDANPLVVGSGVRTGMRIVYDN
                     PREVGADRVVDAVAAQRLYGPPPLIVVDLGTATVLDAINAAGDYVGGAIAPGLSIASE
                     ALFERASKLYRVELEPPRNAIGRNTVEAMRSGLVLGAVATIEGLVARFKQELGGEAKV
                     IATGGWAEMMAKQTTSIDIVDRHLTLAGLRMLYEMNRGEPEPVL"
     gene            1506..2882
                     /gene="dnaA"
                     /locus_tag="VNL92_02130"
     CDS             1506..2882
                     /gene="dnaA"
                     /locus_tag="VNL92_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWO93539.1"
                     /translation="MVTEAQRLWNAALGQLEMQVTRANFETWLRSTEGTAYDGSLLQV
                     SVPTEFTRQWLTSQLTPLIRKTLSSIEGRPLDVAFHVRTNGSREDLRLATDEPRPAPT
                     SASSRPRGTGLLGRYTFDTFVAGASNLLAYSAAQSVCSSPTAIYNPLYIYGGAGLGKT
                     HLLHAIGNELAGQGRAVLYVSAERFTNDYIRAILDRAFEDFRARYRTPEVLLVDDVHF
                     LAGKDQMQEEFFHTFNEVYGQGHQIVLTSDLPPASISLFEDRLRSRFEWGLIVDIQPP
                     DKEMRIAILQAKAQLQGFDLSPEVAEIVASRRQRNIRELEGVLNRLTALARLRGGTIT
                     PAMAEEVLAAIGPDATETDRISPDEFIDAVALYYRVSSDDLRGQGRQKHLALARHVAM
                     YLMREYGKLPLSEIGRHLGKRDHTTVLHGVSKVSASVVRDASLRRDILAIRESLSRRN
                     PPSWKNRA"
     gene            3252..4529
                     /gene="obgE"
                     /locus_tag="VNL92_02135"
     CDS             3252..4529
                     /gene="obgE"
                     /locus_tag="VNL92_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWO93540.1"
                     /translation="MIDEVEIRVKAGDGGNGAVSFRREKFVPRGGPDGGDGANGGSVI
                     LQADPSINSLAAFRRRRVLRAENGQSGRGRKQHGRRGADLVVTVPLGTQVFRQTEEMA
                     YELVSDLNQPAEQVLVARGGQGGRGNARFATSTNQAPRIAQRGSKGEEVTLKLSLKLL
                     ADVGLVGAPNAGKSTLLAAISAAHPKIAPYPFTTLEPELGVVELDYDAFVVADIPGLI
                     EGASRGAGLGLDFLRHIERTRVLVHVLAADAEEALATFHAVNAELAAFDERLGRKPQI
                     VALNKMDMPAAQARAPELEENLRSLGFDVHHISAAAGDGLDDLVRAMHTALQRQASLG
                     EEAEAREVPIPTLRPSPPPGRFRVYRDDGRFVVEGRQPVRLVETMDVDDPEAWAVVLR
                     RLTRMGVVAGLRRAGVRAGDTVRFGEKELRWEG"
     gene            4517..>4726
                     /locus_tag="VNL92_02140"
     CDS             4517..>4726
                     /locus_tag="VNL92_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWO93541.1"
                     /translation="MGGLRRGILGGTFDPVHIAHLILGVEAYRALNLDRVTLVPTGEP
                     WRKTQRLVTAAEHRLAMAKLAVEGDD"
BASE COUNT          881 a         1519 c         1463 g          863 t
ORIGIN      
        1 ctttcccaca tcaaggagtc catcgcaggc gcgaggagga gcggggcgcg cgtagcggcc
       61 gccgtcaggc tcaccatgtc gtcggccagg ccgtgggcga ggcgcgcgag catgctcgcc
      121 gtagccggcg ctaccacgag agcatcggcg gcgcgggcga gctcgacgtg cgcctccgcc
      181 acctccgagt tcacgtcgaa catatcgacc gggacagggc gattcgtgag gctccggaat
      241 gtgaggggcg cgatgaactt cgtcgcctcc tccgtgagga gcacgtcgac catcgcacct
      301 tcttgcacga gccggctcgc gatatcggcc gccttgtacg cggcgatgct gccgctcacg
      361 ccgaggacca cgcggatacc ttcgagaggg cgcctgctca tagaacgggc tccggctcgc
      421 cccgattcat ctcgtagagc atccgcagcc cggccagcgt caggtggcga tcgacgatgt
      481 cgatgctcgt cgtttgcttc gccatcatct cggcccaccc gccagtcgcg atgaccttcg
      541 cctcgccgcc aagctcctgc ttgaagcgcg ccacgagccc ctcgatcgtc gcgacggcgc
      601 ccagaaccaa gccggagcgc atcgcctcga cggtgttcct gccgatggcg ttccgcggcg
      661 gctcgagctc gactcgatac agcttcgagg cgcgctcgaa aagcgcttcg gatgcgatcg
      721 agagcccggg ggcaatcgcg ccaccgacgt agtcgccggc ggcattgatc gcatccagga
      781 cggtggcggt accgagatcg acgacgatga gtggtggcgg gccgtagagc cgctgggccg
      841 ctacagcatc gaccacccga tcggcgccga cctcgcgcgg attgtcgtac acgatacgca
      901 tgcccgtccg cacgcccgaa ccgacgacga gcggattcgc gtcgaagtag cggcgcgcga
      961 ggtcctcaaa aacgggcgtg agcgtcggca caacgctcgc gatgctgcat ttgctcacat
     1021 ccgccggatc gatcttgccc gtccagagaa ggttgaagag caacatggcg tattcgtcgc
     1081 gaagcttgcg gacgtccgtg gagatgcgcc agttggcgcg caactcgtca ccctcgaaca
     1141 cgccgatggt ggtgacggta ttgccaatgt cgagcgccaa gagcacgcga ccacgcctcg
     1201 cagttcctac cggccagacg gctcggcgcc gtcagtatat acgccggcgc atgcgtgaat
     1261 cgcaacggcg gatggcgtcg ggcgtcgcac agcgtttttg gcgaaaccga gcaagtcgtc
     1321 ataagggcct tgatcagcac gtggtgcgat cctatccttc gcgtcatcgg atcgcaccat
     1381 gctacggggc ccggccgctc ggatccccct cccggcggtt gtacgtaact gaactcatgt
     1441 tggtttcaga agcgccagtt gggcttacgc ccagttgagc ttcgctattc cctggaggaa
     1501 tttccttggt cacggaagcg caaaggttat ggaacgcagc gttagggcag ttagaaatgc
     1561 aggtgacacg cgccaacttc gaaacctggc tccgctctac cgaagggacc gcctatgatg
     1621 gttcgctcct gcaggtcagc gtcccaacgg agttcaccag acaatggctg acgagtcagc
     1681 tcacgccact tattcgaaaa acgctgtcgt ccatcgaagg ccgcccactc gatgttgcgt
     1741 ttcacgtccg gaccaacggc agccgggagg acctgcgcct ggcaaccgac gagcctaggc
     1801 ccgcgcccac ctctgcctcc agccggcccc ggggcacagg actgctaggc cgctacacgt
     1861 tcgacacctt cgtcgctggg gcctcgaatc tcctcgcgta ctccgccgct cagagcgtct
     1921 gcagcagtcc taccgccatc tacaaccccc tgtatatcta cggtggagca ggcctgggaa
     1981 agacccatct ccttcatgcg atcgggaacg agcttgccgg ccagggccgc gccgtcctgt
     2041 acgtctctgc ggagcggttc accaacgatt acattcgcgc cattctcgac cgggcgttcg
     2101 aggactttcg ggcccgatac cgcacgccgg aggtcctact ggtcgacgac gtgcacttcc
     2161 tcgcggggaa ggatcaaatg caggaggaat tcttccacac gttcaacgag gtctacggcc
     2221 agggccacca aatcgtgctc accagcgatc tcccgccagc atccatctcc ctgttcgagg
     2281 acaggttgcg ctcgcggttc gagtggggac tcatcgtcga catccagccc ccggacaagg
     2341 agatgcgcat cgccatcctg caagcgaagg cgcaactgca aggcttcgac ctttcgcccg
     2401 aggtcgccga gatcgtcgcc tcgcgacggc agcgcaacat acgagagctg gaaggcgtgc
     2461 tcaatcgact gactgctctc gcgcgactac gtggaggcac gatcaccccc gcgatggccg
     2521 aggaggttct cgcggccatt gggccggacg ctaccgaaac cgatcgcatc tctccggatg
     2581 agttcatcga tgccgtagcg ctctactacc gcgtttccag cgacgacctg cgcggccaag
     2641 gccgtcagaa gcacttggct ctggcccgtc atgtcgccat gtacctgatg cgcgagtacg
     2701 gcaagctccc tctttcggag atcggccggc acctcggaaa gcgcgatcac accacggtgc
     2761 ttcatggcgt atcgaaggtg tcagcctcgg tcgtccgcga cgcatccctc cgacgagaca
     2821 tccttgcgat tcgagaatcc ctctcccgcc ggaaccctcc ttcctggaag aaccgggcct
     2881 gattcttcga aatgttacgg atttgttatg cccatggggc gtaaaccaag gcgtgtctga
     2941 gcacttctct tgacacgccc cttacctttc acgcctcagt gtgtggataa ctcggctgaa
     3001 tcggtggaca ttcccctagg actgtgcata agatcggcct tccgcgggcc acttccacaa
     3061 gcccctcgtc tgtccacacc gcctgttctg caacggactt gtaatgtccc gactaataca
     3121 ctcgccaccc acaggaccat ttgacttcgc tccaactccg cactgcgcgc ttttcctcac
     3181 ccataagatc aagatgatcc ttcaagataa gcactccaaa gtgattcaac atccattcgt
     3241 ataatgattc tgtgatcgat gaggttgaaa ttcgcgtcaa agccggtgat ggcgggaatg
     3301 gagcggtgag cttccggcgc gagaagttcg tgcccagggg tggtcctgac ggtggcgatg
     3361 gtgccaatgg cgggagcgtt atcttgcagg ccgaccccag catcaactca cttgcggcct
     3421 tccgccgtag gcgcgtgctc agggcagaga atggacaaag cggtcggggt agaaagcagc
     3481 acggccggcg cggcgcagat ctcgtcgtga cggtgcctct cgggacgcag gtctttcgac
     3541 aaaccgagga aatggcgtac gagctcgtct cggacttgaa ccagcccgcg gagcaagtgc
     3601 tcgtcgcgcg cggaggccag ggtgggcggg gcaacgcccg cttcgcgact tccacgaatc
     3661 aggcccctcg aatcgctcag cgggggagca agggcgagga ggtgacgctc aagctaagct
     3721 tgaagctttt ggcggacgtt ggcctggttg gagcgccgaa cgccgggaaa tcgacgctat
     3781 tggcggcgat ttcggctgct caccctaaga tcgcgcccta tccgttcact acgctcgagc
     3841 ccgaactcgg cgtcgtggag ctcgactacg atgcattcgt ggtcgcggac attccagggc
     3901 tcatcgaggg ggcgagccgg ggagctggcc taggcctgga cttcctgcgg cacatcgaac
     3961 ggacgcgcgt tctcgttcac gtcctcgctg ctgacgccga ggaagcactg gcaacgttcc
     4021 atgccgtcaa cgccgagctc gccgctttcg atgagcgact ggggcgcaag cctcagatcg
     4081 tggcgctcaa caagatggat atgccggcag cccaggcgcg ggctccggag ctggaggaga
     4141 accttcgaag cctggggttc gatgtgcacc acatctctgc tgccgctgga gatgggttgg
     4201 atgacctggt acgggctatg catacagcgc tccagcgcca agcctcattg ggcgaagaag
     4261 cggaggcgcg ggaggtgccg atccccacgc tccggccctc tccacctcca ggtcgctttc
     4321 gcgtctatcg agacgatggt cgattcgtcg tcgagggtcg gcagcctgtc cggctcgtgg
     4381 aaacgatgga tgtagacgac ccggaggcgt gggctgtggt cttgcggagg ctcacacgca
     4441 tgggtgtcgt agccggccta cggcgcgccg gcgtccgggc gggcgatacc gtgcgatttg
     4501 gcgagaagga actgcgatgg gagggctaag gcgtggcatc ctcggcggaa ccttcgatcc
     4561 cgtccacatc gcgcatctca tccttggagt ggaagcctac cgagcactca acctcgatcg
     4621 tgtgaccctc gttccgaccg gcgagccatg gcgcaagacg cagcgactcg taacggcggc
     4681 cgaacatcgg cttgccatgg cgaagttggc tgtggaggga gacgat
//
LOCUS       DATMBB010000100         4945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_475952, whole genome shotgun sequence.
ACCESSION   DATMBB010000100 DATMBB010000000
VERSION     DATMBB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475952"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VNL92_02145"
     CDS             complement(<1..291)
                     /locus_tag="VNL92_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HWO93542.1"
                     /translation="MKKKSPNRRNLPPALQRELLGILKGRFEKNAGRHPGLRWEAVQA
                     RLEANIEKLWSLNEMETSGGEPDVVGHDPKTGEYVFYDCSAQSPKGRRSLCYD"
     gene            complement(372..656)
                     /locus_tag="VNL92_02150"
     CDS             complement(372..656)
                     /locus_tag="VNL92_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWO93543.1"
                     /translation="MTNVGVDQDAVARSWRERGFSCGLWVDPPGQVWADYVHDVDELV
                     MVVDGDVEFEIDGKVHRPAPGEELFIPARARHTVRNLGRGESRWLYGYRR"
     gene            complement(653..838)
                     /locus_tag="VNL92_02155"
     CDS             complement(653..838)
                     /locus_tag="VNL92_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWO93544.1"
                     /translation="MYRDHMNVGLFNGVVLPDPAGLLEGSGKFMRHVKVRPGSEPDPT
                     ALSALIAAAYRDMKARL"
     gene            complement(1162..4848)
                     /locus_tag="VNL92_02160"
     CDS             complement(1162..4848)
                     /locus_tag="VNL92_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93545.1"
                     /translation="MFHLTARVAWHDSRWNGTVCRQPSCNSFCAALDRIREERDDARE
                     DALAGKRWSTLDPDELPACKAESGAFMNEEEWTRRFVHPYAGIKKADETHGHLKPTLV
                     KVPPYATFVVPFAWMLRSEQDAIDERLPTPLPPDEDSPFTSPWVFGRARQEAILKLFS
                     SRLTPERSLVFFYCKDGHPLGDTISRLVIGVGRIAALLPPKAYDVTGNKPTHLMWDLL
                     IRHTIRPDGEDGFLLPYHEYLEPTGDASEDARRERLLREIAVPADPAHVRVFSYAAEL
                     APADIALSTLVRCLESVRKIREHGIAKGPWERREEWLNQQIALAWQDRGPFPGLGPAL
                     EALGMRLGTALALELRSSGTVKSDADPWPIVDAVIRGKQKPPQSAYAEDLKAIRETWA
                     GLPDERRALLKLLSRFALTSAQALRWFDPKERAKGTAAKVTDEEILANPYRMAETDLG
                     DWNDSPVSVGLVDRGLLPDATIAAKHPVPAPSTVGSPNDARRLRAALVAVLREASENG
                     DALLSVPEALQRVGSLDLAHPCVIGSDWPSTNRAMLSGVVELVEVAGSGAVPTPALQL
                     AELKGREDRLRSILGRRAGKPTGPIKADWQKLLVTAITDAGGKFDKNNELHRQAIEEQ
                     AVALDRLLSRRLSVLVGRAGTGKTSVMGALMLSDALTKDGILLLAPTGKARVRLGKAT
                     NAEAMTVAQFLHRLGRYDGARQRPKFDGKEKYRKEKTVVIDECSMLTMDDLVAVLEAL
                     DLAHVQRLILVGDPNQLPPIGVGRPFADLVSYLETTDAQAEDGSPLGRALARLSVEVR
                     AAAAASAASDALRLASWFTREQQPVDADRVLSDLELGEKFNDLEIVFWKTPDELRAQL
                     LQAFQRHLGLRSAADVVGFDKALGLDERGWVPFDAPEGSERWQILSPVRMHPHGVHDI
                     NRWVQRQFRAKELEAASTAWGVSLGDESIVAKDKVIQTANQRRKAYNWTERATEEHYV
                     ANGEVALIAKVKNMLKAVFAGRPNLTFDYWPNQFSDGYAPLELAYALTVHKAQGSEFK
                     KVFVILPKNCRLLSRELVYTALTRSREQLVLLIEGDDATALFDLTRPERSETARRNTN
                     IFQGVIRVGEDAVPFAEHLIHRTEKGHLVRSKSELVIANMLFQLGIPYEYERVYDGTA
                     APGRLRPDFSFVTADGDLIVWEHLGMLSRPDYKRGWDWKRAWYQKNGFAEGKTLFTSA
                     EEDGKGLDSAELKKTALTIKSLLE"
BASE COUNT          808 a         1661 c         1586 g          890 t
ORIGIN      
        1 gtcgtagcag aggcttctcc ggcccttcgg gctctgcgcc gagcagtcgt agaagacgta
       61 ctcgccggtc ttcggatcat ggccgacgac gtcgggctcg ccgccgctcg tctccatttc
      121 gttcagggac cagagcttct cgatgttggc ctcgagcctc gcctggaccg cttcccagcg
      181 cagcccgggg tggcgcccgg cgttcttctc gaagcggccc ttcaggattc caagcagctc
      241 ccgttggagc gccggcggca ggtttctcct gttggggctc ttcttcttca tgaggtcagc
      301 ccagtatgcc tcgccggcgc aacgaagtgc tccgcagcgt cgcgcgggtc ccccgatggc
      361 gtcgatggtc gtcatcgccg gtagccgtag agccagcgcg actcgccacg cccgaggttc
      421 cggaccgtgt gcctcgcgcg tgccgggatg aagagctcct cgccgggcgc ggggcgatgc
      481 accttgccgt cgatctcgaa ctcgacgtcg ccatcgacga ccatcacgag ctcgtcgacg
      541 tcgtgcacgt agtccgccca gacctggccc ggcggatcga cccagagccc gcacgagaat
      601 ccacgctcgc gccagctgcg cgccaccgca tcctgatcca cgccgacgtt ggtcatagcc
      661 gcgccttcat gtcccggtac gcggccgcga tcagggcgct caacgccgtg gggtcgggct
      721 cagacccggg cctcaccttc acgtggcgca tgaacttgcc gcttccttcg agcaggcccg
      781 caggatcggg caggacgacg ccgttgaata gcccgacgtt catgtggtcg cggtacacgt
      841 tgacgtatcc gaacgccgcg tcctggacgc aggctgtcgg agccccgtgt gcatcagctc
      901 ccgaacgtcg tcgccgcacg cccgcatcgt ctcgaaccac ttgcgcgcga gcggacgaaa
      961 ctcgccgggc tgccgatcca gccaggcatc gatggccgga tcccgcgctg ccgcctcggg
     1021 gaatcgcagc acgcgcatag gagggtcgtc ggcacgctgc tttgaggccg cacgaacacc
     1081 tcccgtctgc aacgccttct tcacgccctc aggtttctcg cgacccgcgc gcgtctgcaa
     1141 gcgggacgtc cgatccgcct actactcgag tagcgacttg atggtgagcg ccgtcttctt
     1201 cagctccgcg gagtcgaggc ccttcccgtc ctcctccgcg gatgtgaaca gcgtcttgcc
     1261 ctcggcgaag ccgttcttct ggtaccaggc ccgcttccag tcccaccctc gcttgtagtc
     1321 gggacgactc agcatcccga ggtgctccca gacgatcaga tcgccgtcgg ctgtcacgaa
     1381 cgagaaatcg gggcgaaggc ggccaggcgc tgccgtgccg tcgtagaccc gctcatattc
     1441 gtaggggatg ccgagctgga agagcatgtt cgcgatgacc agctccgact tgctgcggac
     1501 gaggtgcccc ttctcggttc ggtggatgag atgctcggcg aacggtacgg cgtcttcccc
     1561 gacgcgaatg acaccttgga agatgttcgt gtttcggcgc gcggtctccg agcgctccgg
     1621 acgcgtgagg tcgaagagcg ctgtcgcgtc gtcgccctcg atcaagagca caagctgttc
     1681 gcgcgagcgc gtgagcgccg tgtagacaag ctcgcgcgag agcagccgac agttcttcgg
     1741 caggatcacg aagaccttct tgaactcgct gccctgcgcc ttgtgcacgg ttagcgcgta
     1801 cgccagctca aggggtgcat agccgtcgga gaactggttc ggccagtagt cgaacgtgag
     1861 attgggccgc ccggcgaaca cggccttgag catgttcttc acctttgcga tcagcgccac
     1921 ctcgccattc gcgacgtagt gctcctcggt cgctcgttcg gtccaattgt aggctttgcg
     1981 cctttggttc gcggtctgga tgaccttgtc cttggcgacg atgctctcgt caccaaggct
     2041 gaccccccag gccgtcgacg ctgcttcgag ttctttggcc cggaactggc gctgcaccca
     2101 cctattgatg tcgtgcacgc cgtgcgggtg catgcggacc ggagacagga tctgccatcg
     2161 ctcggaccct tccggcgcgt caaagggcac ccagccgcgc tcgtcgagcc cgagcgcctt
     2221 gtcaaaacca accacgtcgg cggcgctgcg cagcccgaga tggcgctgga aggcctgaag
     2281 gagttgtgca cgcaactcgt caggcgtctt ccaaaacacg atctcgaggt cgttgaactt
     2341 ttcgcccaac tcgaggtcgc tgagaacgcg atcagcgtcg acaggctgct gctcccgcgt
     2401 gaaccaagat gcgaggcgca acgcgtctga ggccgctgaa gccgccgccg ccgcgcgaac
     2461 ctcgaccgag agccgagcga gcgctcgccc gagcggcgat ccgtcctcgg cctgagcatc
     2521 ggtcgtctcc agatacgaga cgagatctgc gaatggacgc ccgacgccga tgggcggaag
     2581 ctgattggga tcgcccacga ggatgagcct ctgcacgtgc gcgaggtcga gcgcttcgag
     2641 aacagcgacg aggtcgtcca tcgtcagcat cgagcattcg tcgatgacga cggtcttctc
     2701 cttccggtac ttctccttgc cgtcgaactt cgggcgctgg cgagcgccgt cgtaccgacc
     2761 gagccggtgg aggaattgcg cgacggtcat ggcctccgca ttcgtggcct taccgagccg
     2821 cacacgcgcc ttgccggtcg gggcgagcag gaggatgcca tccttggtga gcgcatcgga
     2881 caacatcaac gcgcccatca ccgacgtctt gccggtgccc gcgcgcccga cgagcacgct
     2941 caggcgccgc gatagcagac ggtcgagcgc gacggcctgc tcctcaattg cctggcggtg
     3001 cagctcgttg ttcttgtcga acttgccgcc cgcgtcggtg atcgcggtga cgagcaactt
     3061 ctgccagtcc gccttgatgg gccccgtcgg cttgccggca cgcctcccga gaatcgagcg
     3121 caatcgatct tcacgcccct tgagctcggc gagttgcaac gcgggcgtcg gtaccgctcc
     3181 gctgccggcc acctccacga gttcgacaac accggagagc atcgcgcggt tcgtgctggg
     3241 ccagtcagaa ccgatcacgc acggatgagc gaggtcgaga gagccgaccc gttgcagggc
     3301 ctcggggacg ctcaagagcg catcaccatt ctctgacgcc tcacgaagca cagccaccag
     3361 cgcggcgcga agacgacgcg cgtcgttcgg cgatcccacc gtcgaaggcg ccggtacggg
     3421 atgcttcgca gcgatcgtcg catcgggaag caaaccacgg tcgacgagac ccaccgacac
     3481 gggcgaatcg ttccagtcgc cgagatctgt ttcggccatc cggtacgggt tcgcgagaat
     3541 ctcttcgtcg gtcaccttcg cggcagtgcc cttcgccctt tctttcggat cgaaccagcg
     3601 aagcgcctgt gccgacgtga gcgcgaaccg cgacagcagc ttgagaagcg cgcggcgttc
     3661 atcgggaagg cccgcccacg tctcccggat tgccttcagg tcctcggcgt acgccgactg
     3721 gggcggcttc tgcttgcctc gaatgactgc gtccacgatg ggccacgggt ccgcatcgga
     3781 cttcaccgtt cccgacgacc ggagttcgag cgccagagcc gtcccgagcc gcatcccgag
     3841 cgcctcgagc gcggggccga gtccggggaa cggtcctcgg tcctgccatg ccagcgcgat
     3901 ctgctggttg agccactcct cgcggcgctc ccagggaccc ttggcgattc cgtgctcgcg
     3961 aatcttccga accgactcga ggcaccgaac gagcgtcgac agcgcgatgt ctgcgggcgc
     4021 gagctctgcc gcgtaggaga agacgcgaac gtgtgccgga tcggcgggca cggctatttc
     4081 gcgtaggaga cgttctcgcc gcgcatcttc cgaggcatcg ccggtcggct ccaggtattc
     4141 gtggtacggg agcaagaagc cgtcttctcc gtcaggacgg atcgtgtgac ggatgaggag
     4201 gtcccacatc aggtgcgtgg gcttgttccc agtgacgtcg tacgccttcg gcggcaacag
     4261 ggctgcgatt cgaccgaccc caatgacgag ccgtgagatc gtgtcgccaa gcggatgtcc
     4321 atccttgcag tagaaaaaaa ccagcgagcg ctcgggtgtg agccggctcg agaacaactt
     4381 gaggatggcc tcctgccgcg cgcgtccgaa aacccacgga ctcgtgaacg gcgagtcctc
     4441 atcgggggga agcggtgttg gcagccgttc gtcgatggcg tcctgctcgc tgcggagcat
     4501 ccaggcgaac ggcacgacga acgtggcgta gggtggaacc ttcacgagcg tcggcttgag
     4561 gtggccgtgc gtctcgtcgg cctttttgat cccggcgtag gggtggacga agcgacgcgt
     4621 ccactcctcc tcgttcatga acgcgcccga ctcggcctta caagctggaa gctcgtcggg
     4681 gtcgagcgtg ctccatcgct tccccgccag cgcgtcctcg cgagcgtcgt cgcgctcctc
     4741 ccggatacgg tcgagagcag cgcagaacga gttgcacgat ggttgccggc agaccgtgcc
     4801 gttccaacga gagtcgtgcc aggctacgcg cgcagtaaga tggaacatcg gcacctcggg
     4861 tggccctgtg gcgaagagtc gattctacgc ctgaggcact cgcaggggca aatgttcgcg
     4921 gttcgttagc cgctcgcgtg ctcgt
//
LOCUS       DATMBB010000101         5616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_460644, whole genome shotgun sequence.
ACCESSION   DATMBB010000101 DATMBB010000000
VERSION     DATMBB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460644"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VNL92_02165"
     CDS             complement(<1..751)
                     /locus_tag="VNL92_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO93546.1"
                     /translation="MAARMEGKVAIVTGASRGIGKAIAEGFAREGAKVAFCARTLHEG
                     EHQLAGSLDQTLAEIRSAGGTAIAVQCDVSDYEACTRMVEETRRELGPIDVLVNNAAL
                     NYFIPFAEYPVNRWVRSFAVDVHAPFWLSQLVLPDMIARKSGAIVNISSRGGMFPGPG
                     PYQPGFRGGVMYGAAKAALERMTQGIAQEVFEHNIAVNALSPDRGVATPGVLFHKLLE
                     SEDDPRGEPMEYMVEAAILLATRDASYTGHIT"
     gene            complement(884..1351)
                     /locus_tag="VNL92_02170"
     CDS             complement(884..1351)
                     /locus_tag="VNL92_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWO93547.1"
                     /translation="MITAVDTNVLLDVLIPGAPHGEDSERVLGEAVRAGALVISEPVY
                     AELAVHFAARGDLDRFLADTSIRIGPSDAEALYTAGRAWSEYLRRRPASLVCSQCGAL
                     QQVSCVRCGASMQPRQHVVADFLIGAHALLHAGRLLTRDRGFYAKYFPGLKLG"
     gene            complement(1348..1575)
                     /locus_tag="VNL92_02175"
     CDS             complement(1348..1575)
                     /locus_tag="VNL92_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWO93548.1"
                     /translation="MIRAKITGKGQVTVPKDVRERMGLRPGDEIEFVEDAEGFRVQKH
                     VLASPFTKYRGYLKELAGRDPDELVEQMRGR"
     gene            1845..3500
                     /locus_tag="VNL92_02180"
     CDS             1845..3500
                     /locus_tag="VNL92_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWO93549.1"
                     /translation="MASKSKQGWLETVWAKAVSRVKERRERFVTSSGATIDPIYAPDD
                     LEGWSYDEKLGYPGEFPFTRGVQPTMYRGRFWTMRQYAGFGDAEESNRRYRYLLHQGQ
                     TGLSVAFDLPTQIGYDADDPMAEGEVGKVGVSICSIRDMETLFDGIPLEDVTTSMTIN
                     ATAPILLALYVAVGKKQGADLSKLGGTIQNDILKEYIARGTYIFPPKPSMRLITDVFA
                     YCRDELPAWNTISISGYHMREAGCTAAQEVAFTIADAIAYVQSALDAGLSFDEFAGRL
                     SFFFACHNNFLEEVAKFRAARRLWAHIARDRFGAQDSRSMMMRFHTQTGGATLTAQQI
                     DNNVVRTTVQALAAVLGGTQSLHTNSMDEALALPSEHAVQVALRTQQVLAYESGVADT
                     VDPLAGSYFIEHLTDEIEQEARDYIDRIDKIGGAVVAIEHGFQQREIQESAFRLQMEI
                     ERGERIIVGVNRFQTEEPPYEDILRVDPRVGERQRARLAQLRAERDGAAAERALADIE
                     AAAQGTQNLVPLFVTAVENGVTVGEICGVLRGIWGEHKESLVI"
     gene            3541..4374
                     /gene="mce"
                     /locus_tag="VNL92_02185"
     CDS             3541..4374
                     /gene="mce"
                     /locus_tag="VNL92_02185"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWO93550.1"
                     /translation="MIRKIHHVGIVVEDLERAYGFYRDVLGLCVAKEDTVPDQGVRAA
                     LMPVGHSEIELLQPITEGTGIQKFLASRGEGMHHLCFETDGIDRELDELKAKGAPLID
                     ERPRPGLAGMIAFLHPKCTHRTLVELAEPPESHEGGHKVHGELRAKELDHVALAVPDL
                     DAAVADYGTLFGLQSGGTLESAEMGLRAVMLPVGSSFVELLTATTPDSPLAQRIATIG
                     GGGMLTLALEVENLDDAISWLRSKACAVSDASPGPIPNTRIARAEPSSTHGVPLQLIQ
                     R"
     gene            4399..4809
                     /locus_tag="VNL92_02190"
     CDS             4399..4809
                     /locus_tag="VNL92_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWO93551.1"
                     /translation="MVTEARKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYTGIR
                     QTPEMIAEAALQEDVDAIGLSILSGAHNELFPRVVEALRARGIDDVLLFAGGIIPPED
                     VDALKQLGFQAIFGPGSPTSEIIEFVRANVGDRV"
     gene            4806..>5616
                     /gene="meaB"
                     /locus_tag="VNL92_02195"
     CDS             4806..>5616
                     /gene="meaB"
                     /locus_tag="VNL92_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWO93552.1"
                     /translation="MTEELVERLLAGNKRALARALTHVENRTPEGYEILRLVYPKTGR
                     ARIIGITGPAGSGKSTLSSALAREERRRGRSVGIVAVDPSSPFSHGALLGDRIRMQDL
                     AGDPGVFVRSMATRGALGGLAETAGDIASVLDAAGHDVIILETVGAGQDEVDVAGMAQ
                     TTVVVNTPGMGDAVQALKAGILEIADILVVNKADRPSADALVAELEGMLGLSPDSSYQ
                     PPVLRTVATTGEGIVDLADAIDEHHARLAESGDLHRVRLGRARQEVLDAARM"
BASE COUNT          987 a         1911 c         1815 g          903 t
ORIGIN      
        1 aggtgatgtg gcccgtgtag ctggcatcgc gcgtcgcgag cagaatggcg gcctcgacca
       61 tgtactccat cggctccccg cgagggtcgt cctcgctttc caggagcttg tggaagagga
      121 cgccgggcgt cgcaacgccg cggtccggcg agagcgcgtt gaccgcgatg ttgtgctcga
      181 acacctcctg ggcgatgccc tgggtcatcc gttcgagcgc agccttcgcc gcgccgtaca
      241 tcacgccccc acggaaccct ggctggtagg gtcccggtcc cgggaacatc ccgccacggg
      301 agctgatgtt gacgatggcg ccgctcttcc gggcgatcat gtccggaagc acgagctggc
      361 tcagccagaa cggcgcgtgc acatcgaccg caaaggagcg cacccagcgg ttcaccggat
      421 actcggcgaa gggaatgaag tagttgaggg ccgcgttgtt gacgagcacg tcgatgggcc
      481 cgagctcccg ccgcgtttcc tccaccatgc gagtgcacgc ttcgtagtcc gagacatcgc
      541 actgtaccgc gatggccgta ccgccggcgg accgaatctc ggcgagcgtc tggtcgagcg
      601 accctgcgag ctggtgctcg ccttcgtgga gtgtacgagc gcagaacgcc accttcgccc
      661 cctcgcgggc gaagccctcg gcgatcgcct tgccgattcc acggctcgcc ccagtgacga
      721 tcgccacctt gccttccatt ctcgccgcca tcgttgcctc ctcctcagtg atcgcgcgca
      781 agtgtacgcc gctgtgcgcc gcattgccac cacacggtta agtgccccgc gggaggtcat
      841 cccatgcgga ggcctatcgt gtgctaggcg ccgctgcggt tggtcagccc aacttcaggc
      901 cggggaaata cttggcgtaa aagccgcggt ccctcgtgag cagtctgccg gcgtgcagga
      961 gggcgtgggc cccgatgagg aagtcagcca ctacgtgctg cctaggctgc atgctcgcgc
     1021 cgcagcggac gcaactgacc tgttggagag cgccacactg ggaacagaca agcgacgccg
     1081 ggcggcggcg gaggtattca ctccacgccc tgcctgctgt gtacagcgcc tcggcgtccg
     1141 atggcccgat cctgatgctc gtatctgcca ggaatctgtc gaggtcgccc ctggcggcga
     1201 agtggactgc cagctcagcg tacactggct cgctgatgac gagcgcacca gcccgtaccg
     1261 cctccccgag gacgcgctcg gaatcctcgc catggggcgc gccggggatg agcacgtcga
     1321 gcaggacatt ggtatcgacg gcagtgatca ccggccgcgc atctgctcga cgagctcgtc
     1381 cgggtcacgc ccggccagct ccttgaggta gccgcggtac ttcgtgaaag gcgaggcaag
     1441 cacgtgcttc tgaacccgga agccctccgc gtcctcgacg aactcgatct cgtcacccgg
     1501 cctcagcccc atgcgctccc gcacgtcctt cgggaccgtg acctgcccct tgccggtaat
     1561 ctttgctctt atcatggcac gcctcattga cctcggtgta aggatatctc atcttactcg
     1621 acagtaagga acaccggaac gcccagctcg aggacacttc ctcacgtcgc gcgcggcctc
     1681 gatgtctcca gcgcagtttc tggtccacca ccccccggat ctccgtcgtt tgacactcgg
     1741 aaaacggcta cgctacactc accaccgctt cggcgggcag cgcacccgtc cgctcgcgat
     1801 gcccctccca caccgccact catcacacga aagggaatac gaccgtggcc agcaagagca
     1861 agcaaggttg gctcgaaacg gtctgggcga aggccgtctc gcgggtcaag gagcgtcgcg
     1921 agcggttcgt tacgagctcc ggcgcaacca tcgacccgat ctacgcaccg gacgatctcg
     1981 aggggtggtc gtacgacgag aagctcggct accctggcga gtttcccttc acccgcggcg
     2041 tccagcccac gatgtaccgg ggtcgcttct ggaccatgcg tcagtacgcc ggcttcggcg
     2101 acgcggaaga atcgaaccgg cgctatcgct acctcctgca ccagggacag acgggcctct
     2161 ccgtcgcgtt cgacctcccg acccaaatcg gctacgacgc cgatgacccc atggccgagg
     2221 gcgaggtcgg caaggtgggc gtctccatct gctccatccg cgacatggag acgctcttcg
     2281 atggcatccc cctggaggat gtgacaacct cgatgacgat caacgccacc gcgcccatcc
     2341 tgctcgcgct gtatgtcgcg gtcggcaaga aacagggcgc cgacctctcg aagctcggcg
     2401 gcacgattca gaacgacatc ctgaaggagt acatcgcacg gggcacctac atcttcccgc
     2461 ccaagccatc gatgcggctc attaccgacg tctttgccta ctgtcgggat gagctgcccg
     2521 catggaacac gatcagtatc agcggctacc acatgcgcga ggccggctgc acggcggcgc
     2581 aggaagtcgc cttcaccatc gccgacgcca tcgcctacgt gcagtcggcg ctcgacgcgg
     2641 gcctgtcatt cgatgaattc gccggaaggc tctcgttctt cttcgcgtgc cacaacaatt
     2701 tcctcgaaga ggtggcgaag ttccgagcgg cacggcgact ttgggcgcac atcgcccggg
     2761 accggttcgg cgcgcaggac tcccgctcga tgatgatgcg tttccacacc cagaccggtg
     2821 gagcgaccct caccgcccag cagatcgaca ataacgtcgt acggaccacc gtgcaggcgc
     2881 tcgccgctgt gctcggtgga acgcagtcgc tccacaccaa ctcgatggac gaggcgctcg
     2941 ccctgccgag cgagcatgcg gtgcaggtgg cccttcgcac acaacaggtg ctggcctacg
     3001 agagcggcgt agccgacacg gtcgatccgc tcgccggctc gtacttcatc gagcacctca
     3061 cggacgagat cgagcaagag gctagagact acatcgaccg catcgacaag atcggcggcg
     3121 ccgtcgtcgc gatcgagcat gggttccagc aacgcgagat ccaggagagc gccttccggc
     3181 tgcagatgga aatcgagcga ggcgagcgca tcatcgtcgg cgtcaaccgg ttccagacgg
     3241 aggagccgcc ctacgaggat atccttcggg tcgacccgcg ggtcggcgaa cgccagcggg
     3301 cccggctcgc ccagctgcgt gcggagcgcg acggagctgc cgcggagcgg gcgctcgccg
     3361 acatcgaggc tgcggcccag gggacccaga acctcgtccc gctcttcgtg accgcggtcg
     3421 agaacggtgt caccgtgggc gagatctgcg gcgtccttcg cggcatctgg ggcgaacaca
     3481 aggagtcgct cgttatctga aggcgatggc gcttcgccac cgccgaatgg atggaccaga
     3541 atgatcagga aaattcatca tgtcgggatc gttgtcgagg acctcgaacg cgcgtacggc
     3601 ttctaccgcg acgtgctcgg cctgtgcgtc gcgaaggagg acacggtacc cgaccagggc
     3661 gtccgggcgg cgctcatgcc cgtcggccac agcgagatcg agttactgca gccgatcaca
     3721 gagggcaccg gcatccagaa gttcctcgcc tcgcgcggcg aaggcatgca tcatctctgc
     3781 ttcgaaacgg acggcatcga ccgcgagttg gatgagctga aggccaaggg cgcgccgctc
     3841 atcgacgaga ggccccgccc gggcctcgct gggatgatcg cgttcctcca tccgaagtgc
     3901 acgcaccgca ccctcgtcga gctggccgag ccgcccgagt cgcacgaggg tgggcacaag
     3961 gtccacggcg aactccgcgc caaggagctg gaccacgtcg cgctcgccgt acccgacctc
     4021 gatgcggcgg tcgccgatta cggcacgctc ttcggtctgc agtccggcgg gacgctcgaa
     4081 tcggcggaaa tggggcttcg cgccgtcatg ttgccggtgg gcagctcctt cgtcgagctc
     4141 ctgaccgcga cgacgccgga ttcacctttg gcccagcgca tcgcgaccat cggcggtgga
     4201 ggcatgctca ccctcgccct cgaggtcgag aacctggatg acgcgatctc ctggcttcgc
     4261 tcgaaggcat gcgcggtcag cgacgcgagc ccggggccca tccccaacac tcgcatcgcc
     4321 cgcgcggagc catcgtcgac ccacggcgta ccgctccagc tcatccaacg ctgacacctt
     4381 ccgacatgga ggcgaacgat ggttaccgag gcccgcaaga tacgcgttct cgtcgccaag
     4441 cccggactcg atgggcatga ccgcggcgcg aaggtggtag ccagggcgtt gcgcgatgcc
     4501 ggcatggagg tgatctacac cggcattcgc cagacgccgg agatgatcgc ggaggcagcg
     4561 ctccaagagg acgtggatgc catcggcctc tccatcctct ccggggcgca caacgagctg
     4621 ttcccccgag tcgtcgaagc gctgcgtgcg cggggcatcg acgatgtgct gctgttcgcg
     4681 ggcggcatca tcccgccgga ggatgtcgat gctctgaagc agctgggatt ccaggccatc
     4741 ttcgggcctg gctcgcccac ctcggagatc atcgagttcg tgcgcgccaa cgtcggagat
     4801 agggtgtgac ggaagagctc gtcgagcgtc tgctcgcggg caacaaacgg gcgctggccc
     4861 gcgccctcac ccacgtggag aacaggacgc cggagggtta cgagatcctc cgcctcgtct
     4921 acccaaagac tggcagagcg cgcatcatcg gcatcaccgg acccgccgga agcggcaaga
     4981 gcacgctctc gagcgccctc gcacgtgaag agcgacgccg gggccggtcg gtcggcatcg
     5041 tggccgtcga cccgtcgagc ccgttcagcc acggggccct gctcggcgac cgcatccgga
     5101 tgcaagacct tgcgggcgat ccgggcgtgt tcgtccgcag catggcgacg cggggggccc
     5161 tcggtgggct cgcggagacg gccggcgaca tcgcttcggt gctcgatgcc gcgggccacg
     5221 acgtcatcat cctcgagacc gtaggtgccg gtcaggacga agtggacgtg gccggcatgg
     5281 cgcagacaac cgtcgtggtc aacacgccgg gcatgggcga cgccgtgcag gcgctgaagg
     5341 cgggaatcct cgagatcgcc gatatcctcg tcgtgaacaa ggccgaccgg ccgagcgccg
     5401 acgcactcgt cgccgaactg gagggaatgc tcggcctttc gcccgactcc agctatcagc
     5461 cccctgtcct gaggacggtg gcgacgacgg gggaagggat tgtggacctg gcggacgcga
     5521 tcgacgagca tcacgcgagg ctagccgaga gcggcgacct gcaccgggtc cggctcggcc
     5581 gggcgcgaca ggaggtgctc gatgcggctc gcatgg
//
LOCUS       DATMBB010000102         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_380830, whole genome shotgun sequence.
ACCESSION   DATMBB010000102 DATMBB010000000
VERSION     DATMBB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380830"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VNL92_02200"
     CDS             complement(<1..738)
                     /locus_tag="VNL92_02200"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWO93553.1"
                     /translation="MTLRSERKRVTFGSTEIEYTVRRSHRRRKTVEIAVDPVDGVRVA
                     APARATDAEIEDFVRRRARWILRRLDDVRNGAEPLAPREWITGETLLYLGRHYRLRVL
                     DEDGPGERVRLTSRWLEVRAPHPDTRVLRTDDIARAVEQWYRTCAEQKLRERVQMYAR
                     RLGVTSARILVRAQAKRWGSCSRDGTLRFNWRIIMAPLSVVDYVVAHELCHLLHPNHG
                     RRFWEALAAIMPDHAVRRELLRREGPRY"
     gene            complement(1537..>2046)
                     /locus_tag="VNL92_02205"
     CDS             complement(1537..>2046)
                     /locus_tag="VNL92_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWO93554.1"
                     /translation="TSYEVEEVAQLIERAWDRWGEERSPPRFRGWLEEVSGDVRRWGR
                     LAIVLPDFPWQDHRAIPFLGWLRQGRAKDVTVLVVVEAEAPLPSALRGRFRPVHCETE
                     AERRGRLIARRRLRLGWTRRQMAERAGVPVTTLAKIEGGIEAGVTAEAVRRVAEALGL
                     SPEALNGGT"
BASE COUNT          357 a          711 c          617 g          361 t
ORIGIN      
        1 gtacctcggc ccctctcgtc tgagaagctc gcgccggacg gcgtggtcgg gcatgatggc
       61 ggcgagggct tcccagaagc gcctgccgtg gttggggtgc aacaggtggc agagttcgtg
      121 cgccacgacg tagtccacga cggacagcgg cgccatgatg atgcgccagt tgaaccgtaa
      181 ggtgccgtcg cgactgcagc tcccccatcg cttggcctgt gcgcgcacga gaatccgcgc
      241 cgacgtgaca ccgagccgac gggcgtacat ctgcacgcgt tccctcaact tttgctcggc
      301 gcacgttcga taccactgtt ctacggcccg ggcgatgtcg tcagtccgaa ggactctggt
      361 gtcggggtgc ggcgcgcgaa cttcgagcca ccggctggtg agccggacgc gctcgccggg
      421 gccgtcctcg tcgagtaccc ggagcctgta gtgacggccg aggtacagca gggtctcgcc
      481 ggtgatccac tcgcgtggcg caaggggttc ggcgccgttg cgcacatcgt cgagcctccg
      541 gaggatccac cgagcccggc ggcgcacgaa gtcctcgatc tcggcgtccg tggccctcgc
      601 cggcgcggcc acccgcacgc cgtccacggg gtccacggcg atctcgacgg tctttcgccg
      661 acgatgactg cggcgaacgg tgtactcgat ctccgtggag ccgaaggtga cgcgcttcct
      721 ctcgctcctc agcgtcatgc ctctaggacg cgcatgtttc agaaccaggc cttgaacctg
      781 ctcgccttcc caatcaaagg gtcgtcgcgc agacaggtaa atcctcgtgc ttccaactcg
      841 cggacgactt ctatctccgc ctcgcatcga attaccatcg aaaaatcgtt gaagacgacc
      901 cacgtcagcg atagggcgta ccgtctggcc agatcgacca ttcgaaccag tcagtcagtt
      961 cacccctggt ggcctcggga tgctcaacga cccgccgcca gaacgtaacg aactcgtcac
     1021 tctggcgccc ggcgatgcgc ctctcgtagt cgcgaaccca ctcaagccag tcgcacgggg
     1081 ggacgagcga accgtctttg taggcagctc cgacgtgctc gcgccaaaaa agtcctcaaa
     1141 ggactcgtag accgtgtcgt attcggcgaa catttcgtag gctgcctctc cgaaccgctc
     1201 tcggaattct ttccaggtca tcgggaaggc cttacattca tcaaattcag cgaacagccg
     1261 aacccgcccg aaaggagcaa agactaggcc tttgcaaacg gtagaagctc cgtacacgag
     1321 cacgtcgttg gggactggaa tggcatcgat gttcaggttg tcgtctcgga gcatgttcct
     1381 cacctcccgg ttcgaggaag cggatcctcg atcagcagtg agctcgtcct ctgtactgca
     1441 ccctgccgac cacgatccgg ggcggaagcg ctcgctgccg agctgacgcc tcgatcacgt
     1501 gcgcagcgct cgggtaaggc gagactcgct tctgcgtcag gtgccgccat tgagcgcctc
     1561 cggtgaaagg ccgagcgcct cggccactcg ccgcaccgcc tctgcggtca cacccgcctc
     1621 gatgccgccc tcgatcttcg cgagcgtggt gaccggtacc ccagcccttt cggccatctg
     1681 acggcgggtc cagcccagtc gcagccgccg cctcgcgatt agccggccgc gtcgttccgc
     1741 ttccgtctcg cagtgcacgg gccgaaagcg cccacgaagc gctgagggta atggcgcctc
     1801 tgcctcgacg accaccagca cggtcacatc cttggcccgg ccctgccgca gccagccgag
     1861 aaagggtatt gcccgatggt cctgccaggg aaagtcgggc aacacgatcg ccaggcgccc
     1921 ccatctccga acgtcgcctg agacctcctc gagccagccg cggaaccgcg gcggagacct
     1981 ttcctctccc caacggtccc acgcgcgctc gatgagctgg gcgacctctt ccacctcgta
     2041 cgaggt
//
LOCUS       DATMBB010000103         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_103485, whole genome shotgun sequence.
ACCESSION   DATMBB010000103 DATMBB010000000
VERSION     DATMBB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103485"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VNL92_02210"
     CDS             complement(<1..1066)
                     /locus_tag="VNL92_02210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWO93555.1"
                     /translation="MITFPKLGLAAPILSALEQEGYTTPTPIQSEAIPPIIAGRDLLG
                     IAQTGTGKTAAFALPLLHRLTVTQRSAPRHGCRMLVLSPTRELASQIAESFRTYGRHL
                     DLAVATVFGGVPYQAQIKRLARGVDVLVATPGRLLDHLAGRNLRLDSTEVLVLDEVDQ
                     MLDLGFLKPIRRIVAALPSRRQSLFFSATMPAEIAALAGQLLSDPVKVTVAPAASTVE
                     RVAQQVIHVDSRNKRALLTELLQDARMSRTIVFTRTKRGADRVARHLEQRGIRVAAIH
                     GNKSQRQREEALTALRGSRVRVLVATDIAARGLDVEQVSHVVNFQLPEVPEAYVHRIG
                     RTARAGAAGIAISLCDTSERG"
     gene            complement(1393..2445)
                     /gene="lpxD"
                     /locus_tag="VNL92_02215"
     CDS             complement(1393..2445)
                     /gene="lpxD"
                     /locus_tag="VNL92_02215"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWO93556.1"
                     /translation="MHHPGFFHRAGPFALRTIAEAVGAEPVGDAEGEREIDDLRPLRE
                     AGPSHLTFFENRKYAAHLAATRAAACLLAPRDRGQAPNGTIALATTAPYRAFARALGL
                     FYADALRSKAAASPADPSGRLVHPTAEVGSGVTLEPGAIVGREARIGAGTTVAAGAVV
                     GYRVRIGCDCYVGPGAAITHALIGDRVIVHAGVRIGQDGFGFAMGAQGHLKVPQIGRV
                     IIGDDVEIGANSTVDRGSLSDTVIGEGTKIDNLVQIAHNVAIGRHCIIVAQSGIAGSA
                     ELGDFVIMGAQSGVLGHVRVGNGAQIAGMAHVKDDVEAGERMGGTPARPFKEWARSLA
                     ALRQLARRDVWPLGGA"
BASE COUNT          429 a          820 c          847 g          423 t
ORIGIN      
        1 atccgcgttc gctggtgtcg cagagcgaga tcgcgatgcc ggcagccccc gcccgtgcgg
       61 ttcgcccgat ccgatgcaca taggcctccg gaacctcggg cagctggaag ttcacgacgt
      121 gcgaaacctg ctcgacgtca agtccgcgcg ctgcgatatc cgtcgcgacc aacactcgga
      181 cccttgagcc acgcaaggcg gtgagggcct cctcgcgttg acgctggctc ttgttgccgt
      241 gaatcgccgc cacccggatg ccacgctgct caagatgacg agcgacccga tcggcgcccc
      301 gcttggttcg cgtgaaaacg atcgtgcggg acatccgcgc gtcctggagg agctctgtca
      361 gcaaagcccg cttgttccgg ctgtcgacat ggatgacctg ctgggcgacg cgctcgacag
      421 tcgaggcggc gggagccacc gttaccttca cggggtcgct cagaagctgc cctgcaaggg
      481 cggcaatctc ggccggcatg gtcgccgaga agaacaagct ctggcgccgc gacggcaatg
      541 cggcaacgat gcggcggatc ggcttcaaaa agccgaggtc gagcatctgg tcgacctcgt
      601 cgagcacgag aacctcggtg ctgtcgaggc gaagattgcg accggccaga tggtcgagca
      661 gccggccggg cgtggccacg agaacgtcga caccgcgggc gagcctcttg atctgggcct
      721 ggtagggaac gccgccgaac acagtcgcga cagcgagatc gagatggcgg ccgtaggtgc
      781 ggaagctttc ggcaatctgg ctcgcaagtt cgcgcgtcgg gctcaggacg agcatgcggc
      841 agccgtggcg cggcgccgag cgctgcgtta ccgtcaggcg gtgcagaagg ggaagggcaa
      901 aggccgctgt cttgccggtg ccggtctgag cgatgcccag gaggtcacgg ccggcgataa
      961 tggggggaat cgcctcgctc tggatcggcg ttggcgtggt gtaaccctcc tgttcaagcg
     1021 ccgacaggat aggcgcggcg aggccgagct tgggaaaagt aatcacttgt tttgtagctt
     1081 tcgtggatgg acctgtgctg ccggcgacat cggcgtcgct cctggcacga cacggccgtt
     1141 actttgtgac gctccgcgtg gcctgagacg tcgttcgatg gctgcttagg tgctcgacag
     1201 gcggacgact agggatcttg atgtcccggt cctgttcctt cacgcggcgg agcgccgaaa
     1261 ccgctccgta gagcgattgc ggcttgtcgc acgttgcggc tctcatgtca atgaacgggt
     1321 cgcctccgtc tccgcggccc gacgggcccg ccccatctga gatcgtcagg gatcggtccg
     1381 accctcggcg gctcaagctc cgcccagcgg ccaaacgtct cttcgagcaa gctggcgcag
     1441 ggccgcgagg ctacgcgccc attctttgaa tggccgtgcc ggcgtgccgc ccatacgctc
     1501 ccccgcctcg acgtcatcct tgacgtgggc catgccggcg atctgagcgc cgttgccaac
     1561 cctgacatgg ccgaggacgc ccgactgcgc gcccatgatg acgaagtcgc caagctccgc
     1621 ggagccggcg atacccgatt gagcgacgat aatgcagtgc cggccgatgg cgacgttgtg
     1681 tgcgatctga accagattat cgattttcgt cccctcgccg atgacggtat ccgagagtga
     1741 tccccgatca acggtcgaat tggctccaat ctcgacgtca tcgccgatga tcacgcggcc
     1801 gatctgcggc accttgaggt gcccctgcgc gcccatggca aagccgaagc catcctgtcc
     1861 gatgcgcacg cccgcgtgca cgatgacacg gtcgccgatc agggcgtgag taatcgccgc
     1921 gccgggaccc acgtagcaat cgcaaccgat gcgcacgcga tagcccacca cggccccggc
     1981 agcaacggtc gtgccggcac cgatgcgggc ctccctccct acgattgctc cgggctcgag
     2041 cgtgacgccg ctgccaacct cggcagtcgg atggacgagt cgcccgctcg gatccgcagg
     2101 ggacgcggca gccttggatc ggagagcgtc ggcgtagaac aaccccagtg cgcgggcgaa
     2161 cgcgcggtag ggtgcagtgg tagccagggc gatcgtcccg ttcggggcct gtcctctgtc
     2221 gcggggtgcg agcagacatg ccgccgcccg cgtcgctgcc agatgcgcgg cgtacttacg
     2281 attttcgaag aaggtcagat gactgggccc ggcctctcgc agcggacgca gatcatcgat
     2341 ctcccgctcc ccctccgcat cgcctacggg ctcggcgccg actgcttcgg cgatggtgcg
     2401 cagggcgaat gggcccgcgc gatgaaagaa gccgggatga tgcatcgatg ctcccaatga
     2461 caatgtcgag gtgcgcgggc acctgtgcca ggaagctgtc gcctggtgag gcgacagcc
//
LOCUS       DATMBB010000104         3614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_345751, whole genome shotgun sequence.
ACCESSION   DATMBB010000104 DATMBB010000000
VERSION     DATMBB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345751"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VNL92_02220"
     CDS             <1..327
                     /locus_tag="VNL92_02220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93557.1"
                     /translation="VSVSVAGGAGILLAGIEAMAAGLPLVAWDTPMYRQFIEHGRTGM
                     LVPQGDVQALAAALASLLADAPRRRELGQLAHAAAKAYDWSVAAARLIEVLDRVWDVD
                     RQPERP"
     gene            361..1830
                     /locus_tag="VNL92_02225"
     CDS             361..1830
                     /locus_tag="VNL92_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWO93558.1"
                     /translation="MIDRAIARLPQATPLAALKIPVAAALLLVVLALLATGVGGAELL
                     AGLTALLIVLAAAVLDRRLLVPLIIVALPLEIWEELSPIQFDGSSLVDRLGRSTVLNA
                     GRMAIIALGVYWLLTARSRWEERLPRSGLLVPAFAVGILYVLGSLYSRDTEQGLIFTV
                     TMALHLALLVMIPVFVADRQTLWLCVWAFIGVMWILAFVGIYLQVTDTFLWNPDLGFD
                     DTPRINTTFLDPNIYARVLVIAMVLTIVASFQLPQEWRLAFIAAVLAPCALALIFTNS
                     RSGWLVAAMALLVMIALTPIRPRARAAALTAAAIGALAAAAVGEVVFDAKFLDRLQTL
                     TEGSEALGAREYLIDAAWAMFRDHPVFGLGLGSFQEAFLGPYSHYNLNPELGVSLSHT
                     EVLTILAELGIVGAIIVAFLFVRYGALVVNLYRAASLGDRALAAGFGVSVLVIFVSSQ
                     AEARLLNDPYLWLMFGLTLALEGILRRERGLDPPLPDEA"
     gene            1827..2930
                     /locus_tag="VNL92_02230"
     CDS             1827..2930
                     /locus_tag="VNL92_02230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO93559.1"
                     /translation="MTQRLRLCYVADAGSPHVQKWAGHFALEHDVHVVSFRPGSIPRA
                     RVHVLADLPALSKARYPLEAPRLRALLEHIEPDLVHALHVTSYGFVAALARARPLVVS
                     AWGYDILQAPRRTPLHAWMTRFALSQAQAITATGEALATATRRFAPAGRAVHVVPYGI
                     DLDRFAPADDDRGKSTDEASAPIIGSVKALLPTKGFPYLIKAMPLILERHPNARLVLI
                     GEGPERPRLDRLIASLRLENHVELPGELPNSEVPDQLRCMDVYVQPSLTESFGVSALE
                     ASACGLPVVATDVEGGRDIVRHGETGLLVPPGDARALASAILRLLDDPALAKRMGEAG
                     RRFVRERYNWPDNAARMAAIYNETVEAWRARHR"
     gene            2914..>3614
                     /locus_tag="VNL92_02235"
     CDS             2914..>3614
                     /locus_tag="VNL92_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWO93560.1"
                     /translation="MPAIDELGRKGRTASPTFADGQLPFVSVVVPLRNEGEHIGRCLD
                     ALQRQDYPAHMLEVIAVDGVSTDDTRDVVRSRLPGTRIPIRLLGNPGVTAARGLNIGI
                     REANGGVIIILGGHSEVALDFVSRSVDALWRSEADVVGGRIESVARGAAGEAIAIAMS
                     SPFGVGDARFRTSDSPGFVDTVAFGAYRREVFDRIGLFDEDLERNVDDEFNYRLREAG
                     GHIYLNPAIRSRYYTR"
BASE COUNT          517 a         1291 c         1142 g          664 t
ORIGIN      
        1 gtcagtgtga gcgtcgcggg aggcgcgggc atcctcctcg cgggcatcga agcgatggcg
       61 gctggccttc ccctcgtcgc ctgggacacg ccgatgtacc gccagttcat cgagcacgga
      121 cggacgggca tgctggtccc gcagggcgat gtgcaggcgc tggccgcggc cctcgcctcg
      181 ctcctggccg acgcgccgcg ccgtcgagag ctcggccaac tcgcgcacgc agcggccaag
      241 gcatatgact ggtcggtcgc tgcggcgcgc ctcatcgagg tgcttgatcg cgtatgggac
      301 gttgaccgcc agccggagcg cccctagcat gaggccaccc gcgcagggag acagttcgcc
      361 atgatcgacc gagccatcgc ccgcctgccg caggcgacgc cgctcgccgc actcaagata
      421 cccgtcgccg ccgcgctgct gctcgtcgtc ctcgcgctgc tggccacggg cgtaggcggc
      481 gcggagctcc tcgcgggact cacggccctg ctcatcgtcc tcgccgctgc cgtgctcgac
      541 cgccgcctcc tcgtgccgct catcatcgtc gcgctcccgc tcgaaatctg ggaggagctc
      601 tcacccatcc agttcgatgg atcatccttg gtcgataggc tgggacgatc gaccgtcctc
      661 aatgccggcc gcatggcgat catcgcgctc ggggtctact ggctgcttac ggcacgctcg
      721 cgctgggagg agcggcttcc tcgaagcggc ttgctcgtcc cagcatttgc ggttgggatc
      781 ctctacgtcc ttggctccct gtactcgcgc gacaccgagc aaggtctcat ctttacggtc
      841 acgatggcac tccatttggc cttgctcgtg atgatccccg tcttcgtggc cgaccgccag
      901 accctgtggc tctgcgtgtg ggcattcatc ggcgtcatgt ggattctcgc gttcgtcggc
      961 atctacctgc aggtgacgga caccttcctt tggaatcccg atctcggctt cgacgacacg
     1021 cctcgcatca acacgacatt cctcgacccc aacatctacg cccgcgttct cgtgattgcc
     1081 atggtcctaa ccatcgtggc gtcgtttcag ctgccccaag agtggcgcct cgcgttcatc
     1141 gcggcggtcc tggccccatg cgccttggcg ctgatattca cgaactcgcg ctccggctgg
     1201 ctcgtcgccg cgatggccct gctggtcatg atcgcgctga cacccattcg cccacgcgca
     1261 cgagctgcgg cgctcactgc cgcggcaatc ggcgccctcg cggccgcggc cgtgggcgaa
     1321 gtcgtgttcg atgccaagtt cctcgatcgg ttgcagacgt tgacggaggg cagcgaagcg
     1381 ctgggcgcac gcgaatacct catcgacgcc gcctgggcca tgttccgcga tcacccggtg
     1441 ttcggcctcg ggctcggctc gttccaggaa gccttcctcg gcccgtactc gcactacaac
     1501 ctgaaccctg agctcggagt gtcgctttcg cacaccgagg tcctgacgat cctggccgag
     1561 ctgggcatcg tcggcgccat catcgtcgcg ttcctcttcg tccgttacgg cgcgctggtc
     1621 gtcaacctct accgcgcggc aagcctcggc gaccgggcgc ttgccgccgg gttcggtgtc
     1681 tccgtcctcg tcatcttcgt ctcgtcgcag gccgaggcgc ggctgctcaa cgatccgtac
     1741 ctttggctga tgttcggcct caccttggcg ctcgagggca ttctgcggcg ggagcgcggg
     1801 ctcgatccgc cattgcccga tgaagcgtga cgcagcgatt gcgcctctgc tacgtggccg
     1861 atgcgggctc accgcacgtg caaaagtggg cggggcattt cgcgctggag cacgacgtgc
     1921 acgtcgtctc gtttcgtcct gggtcgattc ctcgtgctcg tgtccacgtg ctggccgacc
     1981 ttccggcgct gtcgaaggcg cgctatccgc tggaggcgcc gcgccttcgc gccctcctcg
     2041 agcacatcga gcccgacctg gttcacgcgc tccacgtgac gagctatgga ttcgtcgccg
     2101 cactcgcccg ggcgcggccc ctcgtggtct ccgcttgggg ctacgatatc ctgcaggccc
     2161 cgcggcgtac gcccctacat gcgtggatga cccggttcgc cctaagccaa gcccaagcga
     2221 tcaccgccac cggcgaggca ctcgcgacgg cgacccgtcg cttcgcgccg gcaggtcgcg
     2281 ccgtccacgt cgtgccctac ggcatcgacc tcgatcgctt cgcccctgcc gatgatgacc
     2341 gggggaagag caccgacgag gcgagcgcgc cgatcatcgg ctcggtgaag gcgctcctgc
     2401 cgacgaaagg gttcccgtac ctcatcaaag ccatgcccct gattctggaa cgccacccga
     2461 acgcgcggct cgtcctcatc ggcgaaggcc cggagcggcc ccgcctcgac aggctcatcg
     2521 cgtcgctccg gctggagaat cacgtcgagc tacccggcga gctgccgaat agcgaagtgc
     2581 ccgaccagtt gcgctgtatg gacgtctacg tccaaccctc gctgacagag agcttcggcg
     2641 tctcggcgct cgaggcctcg gcttgcgggc tgcccgtcgt ggcgaccgac gtcgagggcg
     2701 gacgcgacat cgtgcgccat ggcgagaccg gcctgctcgt cccgcccggt gatgcgcggg
     2761 cgctggcatc ggccattctc cgcctcctcg atgacccagc cctcgccaag cgaatgggcg
     2821 aggcaggtcg ccgattcgtg cgagaacggt acaattggcc cgacaacgcc gctcgcatgg
     2881 cggcgatcta caacgagacc gtggaggctt ggcgtgcccg ccatcgatga gctcggtcgc
     2941 aagggccgga ccgcgtcgcc gacgttcgcc gatggacaac tgccgttcgt cagcgtggtg
     3001 gtgccgctcc gaaacgaggg cgaacacatc ggccgctgct tagacgcgct ccagcggcag
     3061 gattaccccg cccatatgct cgaagtcatc gctgtggatg gcgtctccac cgacgatacg
     3121 cgcgacgtgg tccgatcgcg gctgcccggc acacgcatcc ccatacggct actcggcaac
     3181 cccggcgtca cggcggcgcg cgggctgaac atcggcatac gcgaggcgaa cggcggggtc
     3241 atcatcatcc tcggcggaca cagcgaggtc gccctcgact tcgtcagccg ctcggtcgat
     3301 gcgctttggc gctcggaggc ggatgtcgtg ggcggccgca tcgagagcgt ggcgcgcggg
     3361 gccgccggcg aagctattgc catcgccatg tcctcgccgt tcggcgttgg tgatgcccgc
     3421 tttcgtacga gtgactcgcc cggcttcgtc gatacggtag ccttcggcgc ttaccggcgt
     3481 gaggtattcg accgcatcgg gctcttcgac gaagatctgg aacgcaacgt cgacgatgag
     3541 ttcaactacc gcttacgcga ggctggtggc cacatctacc tcaatccggc catccgctcg
     3601 cgctactaca cccg
//
LOCUS       DATMBB010000105         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_142722, whole genome shotgun sequence.
ACCESSION   DATMBB010000105 DATMBB010000000
VERSION     DATMBB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142722"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1716
                     /locus_tag="VNL92_02240"
     CDS             205..1716
                     /locus_tag="VNL92_02240"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWO93561.1"
                     /translation="MRINVHRGEVSRASVDAIAVAVYQGEQKLSGPAKTVDKALDGAI
                     SDLLKSGEVRGKPSELTLVHTLGRMSSPRVLLVGLGKPEKLTTDVVRNAAADAARRAR
                     STGARSLAFEAFGLAQRLLEPSPLGAALVEGAMLGLYEFDRHKSNHEEPKRAIDILSV
                     YVDDGKAKDAIERGAVRGRILAEATNFARDLANEPANHLTPSAMAERARSMAKEHGLD
                     CRILERADMEKLGMGSALSVANGSVQPPKFIVLEYRGGGRKTHTLGLVGKGITFDTGG
                     ISIKPAAGMEEMKGDMSGGAAVIAAMQAIAQLKPKLNVTALVPATENMPSGSATKPGD
                     VVRAMNGKTIEVINTDAEGRLILADALAYANQLGLSPLIDVATLTGACAVALGNVATG
                     AMTNDEALLRDLMAASEQTGEKLWQLPMFDEYEEQIKSDVADIKNTGGREAGAITAAR
                     FLAHFAGDTPWVHLDMAGTDTSKRDRGVWTKGATGIPVRTLVSFALAWAEKQR"
     gene            1736..>2853
                     /locus_tag="VNL92_02245"
     CDS             1736..>2853
                     /locus_tag="VNL92_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93562.1"
                     /translation="MNIEPGNSGGPAGDESGEAWRQNLLAVWFVVFISISGANLVFPF
                     MPLYLEEDLGIESDSAIALWAGLLGAATGAMMFVFSPIWGSLADRYGRKAMLLRAVLG
                     AMTVMFLQGLAQEPWQLLVLRGLQGMFAGTTGAAIALVAAGTPRRHLGYAMGLVQTGQ
                     FTAQMFGPLTGGLLAAAIGFRLTFVATASLYAIGAMLVLLLVKESPASRAPPTADVRG
                     PLSTIGANLSEVARQRSLLLVIGLLSLIFLSTTLVRPILSIVVDQATDERVAFSAGVV
                     FAAFAFTSAIASFGVGRATAAVGYRRWLILMMLGAASFYLPVALVSSVALLAVLMGVV
                     GLFSGAMLPTTNALIGALSPEGKQGSAFGLATSAQALT"
BASE COUNT          487 a          975 c          907 g          484 t
ORIGIN      
        1 agcgtcccca tgtcaacgca tagggacaac agcacgtagt gtcgccgcta ggccgcgtag
       61 actcgttgcg gtggcttgcg tgttgccggt gccctcagcc gcttctacaa taggcccgat
      121 ccccatccgg gccgatcctc agcatcatca actcgcagta tctttcccac tgcgaggccg
      181 atcactctga cgcgggaggc gagcatgcga atcaatgtgc accgcggaga agtctctcgc
      241 gcatcggtcg acgccatagc tgtcgccgtc taccagggcg agcagaagct gagcggcccc
      301 gccaagacgg tcgacaaggc gctcgacggc gccatcagcg atttgctgaa gagcggcgag
      361 gtacgcggga agccctcgga gttgaccctc gtccacacgc tcggccggat gagctcgcct
      421 cgcgtcctgc tcgtgggact cggtaagccg gagaagctga cgaccgacgt cgtgcggaac
      481 gccgcagcgg atgccgctcg ccgggcgcgt tcaacgggcg cccgcagtct cgccttcgag
      541 gcattcggtc tcgcacagcg cctcctcgaa ccgtctcctc tgggcgccgc gctcgtcgaa
      601 ggggcgatgc tcggcctgta cgagttcgac cggcataagt cgaaccacga ggagccgaag
      661 cgagccatcg acatcctctc ggtttacgtc gacgacggca aggcgaaaga cgcaatcgag
      721 cgcggcgctg tccgcgggcg aatcctcgcc gaggcgacga acttcgcccg cgacctggcg
      781 aacgagccgg ccaaccacct cactccctca gccatggcgg agcgtgctcg gtccatggcg
      841 aaggaacacg gtctggactg ccgcattctc gagcgagccg acatggagaa gctcggcatg
      901 ggctcggcgt tgagcgtggc gaacggcagc gtccagccgc ccaagttcat cgtgctcgag
      961 tatcgagggg gcggccgcaa gacccacacg ctcggcctcg tcggtaaggg catcacgttc
     1021 gacacaggcg ggatctcgat aaagccggcc gccggcatgg aggaaatgaa gggcgacatg
     1081 tcgggaggtg ctgccgtcat cgccgcgatg caggccattg cccaactgaa gccaaagctc
     1141 aacgtgaccg cactcgtgcc ggccaccgaa aatatgccct ccggcagcgc gacgaagccg
     1201 ggcgatgtcg tgcgcgccat gaacggcaaa acgatagagg tgattaacac cgacgcggag
     1261 ggccgcctca tcctcgccga cgcgctggcg tatgcgaacc agctcggcct ctctccgctc
     1321 atcgacgtgg ccacgctcac aggggcctgc gcagtagcgc tcggaaacgt ggcaaccggc
     1381 gccatgacca acgatgaggc gctcttgcgc gacctcatgg cggccagcga gcaaaccggc
     1441 gagaagctgt ggcagctgcc catgttcgac gagtacgaag agcagatcaa gagcgacgtt
     1501 gccgacatca agaacaccgg cggccgcgaa gccggtgcca tcaccgcggc ccgcttcctc
     1561 gcccacttcg cgggcgatac gccgtgggtc catctcgaca tggcgggaac cgatacgtcg
     1621 aagcgcgacc gcggcgtatg gacgaaggga gcgacgggaa tccctgtgcg aacgctcgtg
     1681 agctttgccc tggcctgggc cgagaagcag cgttgattcc cacccacgat gacacgtgaa
     1741 catcgagcct gggaacagcg ggggcccagc aggggacgaa tccggcgaag cctggcgcca
     1801 aaacctcctc gctgtctggt tcgtcgtctt catctcgatc tcgggcgcga acctcgtgtt
     1861 ccccttcatg ccgctctatc tcgaggagga tctcgggatc gagagtgaca gcgctatcgc
     1921 tctgtgggcc ggcctcctcg gagccgccac aggcgcgatg atgttcgtct tctctcccat
     1981 ctggggctcg ctcgccgacc ggtacgggcg caaagccatg ctgctacggg cggttctcgg
     2041 tgcgatgacc gtgatgttct tgcagggcct tgctcaggag ccgtggcaac ttctcgtcct
     2101 gcgtggcctg caggggatgt tcgcaggcac aacgggcgcc gcgatcgcgc tcgtcgccgc
     2161 cggcacaccc aggcgccacc tcggttacgc gatgggcctc gtccaaaccg ggcagttcac
     2221 ggcccagatg ttcggtccgc tgaccggcgg gctgcttgcc gccgcgatcg gcttccgcct
     2281 gaccttcgtc gccacggcga gcttgtacgc catcggcgcc atgctggtcc tgctcctggt
     2341 gaaggagtcg ccggcgtcgc gcgccccacc caccgccgac gtccgaggcc ccctttccac
     2401 catcggcgcc aacctttcgg aagtcgcgcg gcagcgcagc ctgctcctcg tcatcggcct
     2461 cctatcgctc atctttctct cgacgacgct ggtacggccc atcctttcga tcgtcgtcga
     2521 ccaagccacc gacgagcgag tcgcgttcag cgccggcgtc gtcttcgcag cattcgcctt
     2581 caccagtgcc atcgcctcct ttggcgtggg ccgagcgacg gccgcggttg ggtaccggcg
     2641 ctggctcatc ctgatgatgc tcggcgccgc aagcttctat ctcccggtcg ctcttgtgtc
     2701 cagcgtcgcc ctgctcgcag ttctcatggg cgttgtgggc ctgttcagcg gcgccatgct
     2761 ccccacgacg aatgcgctta tcggggcgtt gagcccggag gggaagcagg ggtcggcttt
     2821 cgggcttgcc accagtgccc aggccctcac cat
//
LOCUS       DATMBB010000106         2175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_181044, whole genome shotgun sequence.
ACCESSION   DATMBB010000106 DATMBB010000000
VERSION     DATMBB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181044"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VNL92_02250"
     CDS             <1..198
                     /locus_tag="VNL92_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93563.1"
                     /translation="SPGGSTRSYARIDLFLAFCVGLFVMGLASLDLTFPGYVLFAVGL
                     AGTAYALSRLTVRWLTARRER"
     gene            complement(217..1575)
                     /locus_tag="VNL92_02255"
     CDS             complement(217..1575)
                     /locus_tag="VNL92_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWO93564.1"
                     /translation="MMDDIDSYIDQNFDQTVEWLMSLCRLPSVSAQNVAIEETAHHLA
                     TELGELGFSTDVAPKPEGGHPVLLAERDERASRTLLFYNHYDVQPPEPLDLWTTPPFE
                     PAIRDGKLYARGASDNKGNIAARLAALRAILTVRGSLPVNVRFLIEGDEEIGSPNLAP
                     FVERHAQRLRADACLWEGSGVNWQGAPEVTLGVKGLLYVELEVRVATRDSHSSWATVV
                     PNAAWRLAWALASLKDEDERIRIDGFYDDVRPPTREEDAAVAKLPDESAETIRSLGLR
                     QALLGVGGVEFKRRHLFEPTCTIDGLSAGYEGPGAKTVLPAEARAKIEFRLVPDQDPD
                     DIFRKLRRHLDRHGFDDIEARVLSAEAAARTPITAPFVQDVAETARRTYGVEPMLVPT
                     MAGTGPLHAFIRHLGVQSADCGVGYPDSRIHAPDENVRLEDVRRNIHHIAALLLRFGG
                     VE"
     gene            complement(1572..2006)
                     /locus_tag="VNL92_02260"
     CDS             complement(1572..2006)
                     /locus_tag="VNL92_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93565.1"
                     /translation="MNVRRAEGEDRDVLQAWLADALSGATGSRTTATSAAERLLSMIR
                     DRPEDLRIIEVDGRPEGLVKARDTRATCTFDAVAVRPSARGGGIAAKAILEIEDSSRA
                     SRFVGALAPADGRALYFWLRLGYRPVRRPDGRVEMERRRGQQ"
     gene            complement(2003..2173)
                     /locus_tag="VNL92_02265"
     CDS             complement(2003..2173)
                     /locus_tag="VNL92_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93566.1"
                     /translation="MRRRTDRGVLVVLDRRVRSRQYGQAFLDALPDCIIEEPRPGELA
                     ARVQDWLCPKGE"
BASE COUNT          327 a          748 c          726 g          374 t
ORIGIN      
        1 tcccccggcg gttccacgcg cagctacgcg cgaatcgact tgtttctggc gttctgtgtc
       61 ggcctcttcg tgatggggct tgcctcgctc gacctcacgt tcccgggcta cgtgctcttc
      121 gcggtggggc tcgcgggtac ggcctacgcc ctgtcgcgcc tcacggttcg gtggctgaca
      181 gcccggcgag agcgctgagc gcaggctgcg agcgcatcac tcgacgccgc cgaacctgag
      241 cagcagcgct gcgatgtggt gaatgttgcg gcgtacgtcc tcaagcctca cgttctcgtc
      301 cggcgcgtgg atgcgcgaat cgggatagcc gacgccgcag tcggcgcttt gcacgccgag
      361 gtgccggatg aacgcgtgca gcgggcccgt tccggccatc gtgggcacga gcatcggctc
      421 gacgccatag gtgcgacggg ccgtctctgc gacgtcctgg acgaacggag ccgtgatcgg
      481 tgtgcgcgcc gcggcctcgg ccgagaggac gcgcgcctcg atgtcgtcga agccgtggcg
      541 atcgaggtgg cggcgcagct tgcggaagat gtcgtccggg tcctgatccg ggacgagccg
      601 gaactcgatc ttggccctcg cctccgccgg gaggacggtt ttggcgcccg gaccctcgta
      661 cccggcactt aggccatcga tggtacacgt aggctcaaag agatggcgac gcttgaattc
      721 gacgcccccg acgccgagca aggcctgccg caagcccagc gaacggatcg tctcggccga
      781 ttcgtcgggg agcttcgcga ccgctgcgtc ctcctcgcga gtgggcggcc ggacgtcgtc
      841 atagaagccg tcgatgcgaa tccgttcatc ctcgtccttc agcgatgcca aggcccacgc
      901 gagccgccag gccgcattag gcacgaccgt cgcccacgat gagtgggagt cgcgcgtggc
      961 gacccgcact tcgagttcga cgtacaggag tcccttcacg ccgagcgtga cctcgggtgc
     1021 cccctgccag ttgacgccgc tcccctccca gaggcacgcg tcggcccgaa gccgctgcgc
     1081 gtgcctctcc acgaacggcg cgaggttcgg actgccgatc tcctcgtcgc cttcgatgag
     1141 gaagcggaca ttgacgggga ggctgccccg taccgtgagg atagcgcgga gagcggccag
     1201 gcgcgccgcg atgttgccct tgttgtcgga agcgcccctc gcgtagagct tcccatcgcg
     1261 gatggccggc tcgaacggcg gcgtggtcca caggtccagc ggctccgggg gctgcacatc
     1321 gtagtggttg tagaagagaa gcgtccgcga cgcgcgctcg tcgcgctctg cgagaaggac
     1381 gggatgacca ccttccggct tcggcgcgac gtccgtcgag aagcccaact cccctagctc
     1441 agtcgcaaga tggtgcgcgg tctcctcgat ggcgacattc tgcgcgctca cgctcggcaa
     1501 gcggcagagc gacatgagcc actcgaccgt ctggtcgaag ttctgatcga tgtaggagtc
     1561 gatgtcgtcc atcattgctg cccccgtctc cgctccatct cgacacgtcc gtccggtcgt
     1621 cgcaccggcc gatagcctag ccgaagccag aagtagagcg cccgcccgtc cgcaggggcc
     1681 aacgctccga cgaagcgact cgcacgtgaa ctgtcctcta tctcgaggat ggccttcgcg
     1741 gctatcccgc ctcctcgggc ggacgggcga accgcgaccg cgtcgaacgt gcacgttgcg
     1801 cgggtgtcac gtgccttgac caagccctcc ggccttccat ccacttcgat gatgcgcaag
     1861 tcctcgggtc gatcgcgaat catcgaaagg aggcgctccg cggccgaggt cgctgtggtt
     1921 cgggatccgg ttgcgcccga caacgcgtct gcgagccagg cctgtagcac gtcgcggtcc
     1981 tcgccttcgg cgcggcggac gttcattcgc ccttcggaca cagccagtct tgcacccggg
     2041 ccgccagttc gcccggtcgc ggctcctcga tgatgcagtc ggggagtgcg tccaaaaagg
     2101 cctgtccgta ctgccggctg cgcacgcggc gatcgaggac cacgagcacg ccgcggtccg
     2161 tccgccggcg gatca
//
LOCUS       DATMBB010000107         7208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_67459, whole genome shotgun sequence.
ACCESSION   DATMBB010000107 DATMBB010000000
VERSION     DATMBB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67459"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VNL92_02270"
     CDS             complement(<1..199)
                     /locus_tag="VNL92_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93567.1"
                     /translation="MESIVRAAAVYLFLLVVFRLAGRRTLSQLTNFDFVLLLIISEAT
                     QQALLGQDFSMTNAFIVITLLV"
     gene            complement(226..732)
                     /locus_tag="VNL92_02275"
     CDS             complement(226..732)
                     /locus_tag="VNL92_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93568.1"
                     /translation="MAKVHRVGDLEIDEDMSFQRRQWRVERIGWLLMAAFLLAALLGL
                     FGSGVFDDVTFGEKSDPARIEVDRFTRFDAPTRLRVHITSDAVADERVVLFLSRDYLE
                     HVQLEAITPEPESVRLEGVWQVYEFEAEPAEGVIIFTLRPLSAGSLSGRAAIEGSEPI
                     EFDQFVFP"
     gene            860..982
                     /locus_tag="VNL92_02280"
     CDS             860..982
                     /locus_tag="VNL92_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93569.1"
                     /translation="MLQVFIAALVIGALVVADGRALEWTVARWATVRHWMEERS"
     gene            1027..2721
                     /locus_tag="VNL92_02285"
     CDS             1027..2721
                     /locus_tag="VNL92_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWO93570.1"
                     /translation="MSGDGGIDRARRAYARRAWGEARTAYAQASVNAALSLDDYERFA
                     GAAHLVGEDAECREVLAQGFRSALECGDVTRAARFAFWICHGLMFTGEWSQAKGWLAR
                     ARNLLTEQGADCVEWGYLLIPVAVEQMGLLPVANGEHEEDAVSAAHGSFSEALAIGRR
                     FADLTLQAMANHGRGRALIRLGRIDEGMTALDEAMVAVTTGEVLPFAVGNLYCGLLEA
                     CHQTFDVGRAREWTAAMSRWCEGQPDLVPYRGPCLVHRVEVMQLRGDWEDALLEARRA
                     CEWLSLPASPEGAADAYYMLAELHRLRGDVAPAEEAYRKASRLGRPPEPGLALLWLGQ
                     GRAEAARAAVHRALGEVRDEPARRSELLGAYIEILLEVGDVGGAREAADELRQIAAAL
                     DVPLLHALADRADGSVRIAEGDAKAALAPLRRSWMAWQKLDVPYEAARVRVLIGTACR
                     ALGDGESAAMEFDAARWIFEQLKAFPDLARLDTDAPPLAASETSGPLTPRELEVLRMI
                     AAGSTNKAIAATLVISEHTVARHVQNMLSKLGFSSRASLAAFAVEQGLAARGRSQN"
     gene            2872..4041
                     /locus_tag="VNL92_02290"
     CDS             2872..4041
                     /locus_tag="VNL92_02290"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93571.1"
                     /translation="MMTMLSVEELVQPVADDIRRTAAQAEAERRLPEELMAKLRDAGL
                     FSIYTPREFGGLGLALPEALRVVEEVSRHDGSTGWTVALGIANGYFTSAVPEATAAKV
                     LEGGSALIAGAPAFGVRAIPVDGGYRLTGRWGFNSGAPNADWIGCPAPIFDGEVPRLD
                     AHGQPEMVLWFIPPEDAQIIDTWHVTGLRASGTQDLCVENVFVPNEMTGSLSMPAGPH
                     PIRECVVARIPFMTLFCIAQAPPVCLGLARRAIEEFRELAFAKERPPAPRLSEQVPAQ
                     VGLARAEALLGSARSYWYESVQQLWDAAVGGSEPSLEERTKARLASLTVAENSLAAAD
                     LLYRLAGSSAIFQSSPLERCWRDVHTAAQHMHVQDARWETAGRVLFGLDPGSPII"
     gene            4207..4283
                     /locus_tag="VNL92_02295"
     tRNA            4207..4283
                     /locus_tag="VNL92_02295"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4241..4243,aa:Val,seq:cac)
     gene            4292..4379
                     /locus_tag="VNL92_02300"
     tRNA            4292..4379
                     /locus_tag="VNL92_02300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4326..4328,aa:Leu,seq:cag)
     gene            4396..4472
                     /locus_tag="VNL92_02305"
     tRNA            4396..4472
                     /locus_tag="VNL92_02305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4430..4432,aa:Arg,seq:gcg)
     gene            complement(4827..5669)
                     /locus_tag="VNL92_02310"
     CDS             complement(4827..5669)
                     /locus_tag="VNL92_02310"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWO93572.1"
                     /translation="MTSFRIATINTAKGDGPYLERLDALLAGLSDLDLDIIALQEVLL
                     SEDGRLDTARVLARGLGMACAYVPARWKRRVVQGRTVATWSGLALLSRLPIADVATRP
                     LPSTDRDGERRVLLTRVSQGGVCLRIANTHLTHLRDAQQVRRVQLTAVLAESWWHGPA
                     SIRLLCGDLNAGPASDELIAVRERSQGWHVVDAYSAGGGMPRSSVRIPVTASPGEFRI
                     DHIWSLAPSENAHPAFSEARVALTEPAANGVLPSDHLAVAVTVQAPVTVLGPAPATQR
                     RAGD"
     gene            complement(5666..6298)
                     /locus_tag="VNL92_02315"
     CDS             complement(5666..6298)
                     /locus_tag="VNL92_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93573.1"
                     /translation="MAATSGLRTKMKLGPAPLVDPTTVVQESRLGAWTMIAEGCSIIE
                     SEVGDYSYLMDSCHAIYTRIGKFCSIASHVRLNPGNHPLWRVTSHHLTYRRVMYDVGS
                     ADDADFFAWRRDHPVALGHDVWIGHGAIVLPGVTVGTGAAIGAGAVVTRDVEPYTVVA
                     GVPARPIRRRFPEDVAGTLLRIAWWDWDRERIEACIDDFNDLETFLSKHG"
     gene            complement(6384..>7208)
                     /locus_tag="VNL92_02320"
     CDS             complement(6384..>7208)
                     /locus_tag="VNL92_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWO93574.1"
                     /translation="VRSYSEAIRDSGAAQHGIAIELSGWYIEQWFGKAGHLYIDAENG
                     RSGPATAVEFNDDLGRELFTWLDEMVDAGLALNVGRNPMGTEHLLAVGTGEAAMTIAT
                     SAALGSIFQVLESGEFPAGDIEVGVGPMPGIEGDGGILVGGASLWIVNRSSPEEQEAA
                     WTYVKWLNEPEQQARWHEGTGYIPIRKSAVETDIVRQLWTERPQFRIAYDQLLNGETN
                     AASAGPVMGAYREVRDAVVQALERMMLEDVSPEDALSQAEREANAALEEYTSRSGA"
BASE COUNT         1223 a         2307 c         2437 g         1241 t
ORIGIN      
        1 taacgaggag ggtgatgacg atgaaagcgt tcgtcatcga gaagtcttgg ccgaggagtg
       61 cctgttgtgt cgcttcgctg atgatgagca gcaagacgaa gtcgaagttc gtgagttgac
      121 tcaacgttcg gcggccggca agccgaaaca caaccagtag gaagaggtag actgccgccg
      181 ctcgtacgat cgattccacc gttcactccc ttcatcgcgg aaccgctacg gaaagacaaa
      241 ctgatcgaac tcgatcggtt ccgaaccttc gatggcggcg cgcccggaga gcgatcccgc
      301 gctcaatggt ctcagggtaa agatgatgac accctccgcc ggttcggcct cgaactcgta
      361 gacttgccaa accccttcga ggcgcacgga ctctggttcg ggcgtgattg cctccagctg
      421 aacgtgttcc aggtagtcgc ggctgaggaa gaggacgacg cgttcgtccg ctacggcatc
      481 cgatgtgata tgcacccgta ggcgagtcgg cgcgtcgaac cgagtaaagc gatcgacctc
      541 gatgcgagca ggatcgctct tctcgccgaa ggtcacgtca tcgaacacgc ctgatccaaa
      601 gagcccgagc agtgcggcga ggaggaaggc cgccatgaga agccagccga tccgctcgac
      661 cctccactgg cgacgctgaa acgacatgtc ctcgtcgatt tcgaggtcgc caacccggtg
      721 taccttagcc atgcgtcacc taccgcccgc gcggctcggc gggcagctcc agcctaatgc
      781 gccgatgctt ttcgcaatgc aaaggaaagt gcgataatca tcgcgattgc gtacctagtt
      841 gggatcgcga ggcgaccgga tgctccaggt cttcatcgcc gctctcgtca tcggggcgct
      901 cgttgtagcc gatggccgcg cgctcgagtg gacggtcgca cggtgggcaa cggtgcgcca
      961 ctggatggag gagcgctcct gacacgcggg ggctccctga ttgcctcgcc gttgtagaat
     1021 gccgccatgt ccggggatgg ggggatcgat cgagcgcgcc gtgcgtatgc ccggcgggcg
     1081 tggggcgagg ctcgcaccgc ctacgctcaa gcgagcgtca acgcagcact ctccctcgac
     1141 gactacgagc gcttcgcggg cgcagcgcac ctcgtcggtg aggatgcgga gtgccgcgag
     1201 gttctggcgc aggggttccg ctccgccctc gagtgcggcg atgtcacgcg cgccgcgcgg
     1261 ttcgccttct ggatctgcca tggcctgatg tttacgggtg agtggagcca ggcgaagggg
     1321 tggttggccc gggcccggaa cctgctgacc gagcaagggg ccgattgcgt cgagtgggga
     1381 tacctgctca tcccagtcgc tgtcgagcag atggggttgc tgcctgtggc caacggcgag
     1441 catgaggagg acgccgtcag tgcggcccat ggatccttca gcgaggcgtt ggccatcggc
     1501 cggcgcttcg ctgacctcac cctgcaggcc atggccaacc atgggcgtgg ccgcgcgctc
     1561 atcaggcttg ggcgtatcga tgagggcatg acggctctgg acgaggcgat ggttgccgtc
     1621 acgaccgggg aggtactgcc cttcgccgtg ggcaacctct actgcggact tctggaggct
     1681 tgccaccaga ccttcgacgt gggacgggcc cgcgagtgga ccgctgccat gagccgctgg
     1741 tgcgagggac agccggacct tgtcccgtat cgcgggccgt gtctcgtaca ccgcgtcgag
     1801 gtgatgcagc tgcgaggcga ctgggaggat gcgctcctgg aagcgcggcg cgcctgcgaa
     1861 tggctctccc tgcccgcgag cccagaggga gcggccgatg catactacat gctcgcggag
     1921 ttgcaccggc ttcgcgggga cgttgcccct gcggaggagg cgtatcgcaa agccagccgg
     1981 ctggggcgcc cgcccgagcc cggcctcgcg ttgctgtggt tgggccaggg tcgggccgag
     2041 gccgcaaggg ccgcggtgca tcgggccctc ggggaggtca gggacgagcc tgcccgtcgt
     2101 tctgagctgc tgggcgcata catcgagatc ctactggaag ttggtgatgt cggcggagcg
     2161 cgcgaggctg cggatgagct gcggcagatc gccgccgcgc tcgacgtccc acttctgcat
     2221 gcactcgccg atcgagcgga tgggagcgtg cggattgccg aaggggacgc gaaggcagcc
     2281 ctggcgccgc tccggcgctc gtggatggcg tggcagaagc tggatgtgcc ctacgaagcg
     2341 gcgcgtgtcc gtgttctcat cggcacggcc tgccgtgctc tcggcgatgg ggagtcggcg
     2401 gccatggagt tcgacgcggc tcgctggatc ttcgagcagc tcaaagcctt ccccgacctc
     2461 gcgcggctcg ataccgacgc gccgccactg gcggcaagcg agacatccgg tccactcacg
     2521 ccgcgcgagc tggaagtact gcggatgatc gccgccggca gcacgaacaa ggcgatcgcg
     2581 gcgacgctcg tcatcagcga gcatacggtc gcgcgccacg tgcagaacat gctctccaag
     2641 ctcggcttct cgtcgcgggc gagcctcgcg gccttcgccg tcgagcaagg attggctgcg
     2701 cggggccgta gtcagaacta accatcggat gtcttgcagc gaattggtcg atctgcgcga
     2761 tgccgcgcga tcgggtgggc tctatgttgt catcggttcg gctcacatcg ccgccccgca
     2821 ccgagccgaa accccgatcg ggcgcgggaa gatcgaagaa aggagcgcac gatgatgacc
     2881 atgctctcgg tcgaggaact ggtccaaccg gtagcggacg acatccgcag gaccgccgct
     2941 caagcggagg cggaacgccg ccttccggaa gagctcatgg cgaagctcag ggacgccggc
     3001 ctgttctcca tctacacgcc tcgcgagttc gggggcctgg gcttggcgct gccggaagcc
     3061 ctacgcgtgg tggaggaggt gtcgcgacac gatggttcca cgggttggac ggtggcgctg
     3121 ggaatcgcca atggctactt tacctcggct gtgccggagg cgacggccgc aaaggtactc
     3181 gagggcggct cggccttgat cgcgggagca cccgcgttcg gtgtgcgtgc catcccggtc
     3241 gatggtggat accggctgac cgggaggtgg ggcttcaaca gcggcgcacc gaacgccgac
     3301 tggatcggat gtcctgcgcc catcttcgat ggtgaggtgc cgcgtttgga cgcgcacggg
     3361 cagcccgaga tggtcctctg gttcatcccg cctgaggatg cacagatcat cgacacgtgg
     3421 cacgtcacgg gccttcgtgc cagcggcacg caggacctct gcgtggagaa cgtgttcgtc
     3481 ccgaacgaga tgacggggtc gctctccatg ccggccggcc cgcacccgat tcgggaatgt
     3541 gtcgtggctc ggataccctt catgacgctc ttctgcatcg cacaggcgcc gccggtctgc
     3601 ctcggcctgg ctcgccgtgc catcgaggag ttcagggaac ttgcgttcgc gaaggagcgg
     3661 ccgcctgccc cgcgcttgag cgagcaggtg ccggcccagg tcgggctggc gcgggcggag
     3721 gcgttgctcg ggtcggcacg ctcctattgg tacgaaagcg tgcagcaact gtgggatgct
     3781 gccgtgggtg ggagtgaacc ttccttggag gagaggacca aggcgcgcct cgcctcgctg
     3841 accgtcgcgg agaacagcct agccgcagcc gacctcctct atcgactggc tggcagcagc
     3901 gcgatcttcc agtcctctcc gctcgagcgg tgttggcgcg atgtgcacac ggcggcgcag
     3961 cacatgcatg tgcaggatgc gcgctgggaa acggcgggac gggtgctgtt cgggctcgat
     4021 cccgggagtc ccatcatctg aaggctgcgc gtcgctgcat cgacgaagcc ggcagcgggg
     4081 ttactaacgt gactcccacc gccggctttg catctgcaga gtcgcgacga agggaggggc
     4141 ggcgatccgc cgatggtcgg tttgtttgac gaggccaggg gccggggcta aactgaattt
     4201 gctaaggggc ggttagctca gttggtacga gcgcttgctt cacacgcaag aggtcactgg
     4261 ttcgagccca gtaccgccca ccatgagccg cgccgaagtg gcggaactgg cagacgcgct
     4321 acgttcaggg cgtagtgccc cttgcgggcg tgggagttca aatctcccct tcggcaccat
     4381 cgttggtcaa gctccgcgct cgtagctcag ttggatagag cgcagccctg cggaggctga
     4441 ggtcgtgggt tcgagccccg ccgagcgcgc cataagtaca caaatagcgg caggtaggag
     4501 ggcttcggct ccactgccgc taatcgtttg tcgcgaattc gcacctgctc gaacacggtc
     4561 gtgaggagct tgcgctgcag ctccggcgga gcctccggga aaaaccgggg cgagcaatcg
     4621 ctcatgtttc gcggcgagtg gtctccacgt cacgaccgaa tcgtgatgta ctaggacctc
     4681 gttcacactg ccgcttctcg gataatgccg cagctttacg ataacttggt ctcggcatga
     4741 atggacggtt gttgcttcca cattccgatg tgcgtggcgc cgctcggcag cctgccgcta
     4801 cagggtgaca ccggagccgc gggttctcag tcccccgccc tccgttgcgt cgccggcgcc
     4861 gggcctagca ctgtgacggg cgcctgcacg gtgacggcga ccgcgagatg atcgctggga
     4921 agcacaccgt tggcggcggg ctcggtgagc gcgacgcgcg cctccgagaa ggccggatgc
     4981 gcattctccg agggtgcgag cgaccaaatg tgatcgatgc ggaactcgcc ggggctcgcc
     5041 gttacgggaa tgcggacgct cgaccgcggc atgccgccac cagcgctgta ggcatcgacg
     5101 acgtgccaac cctgcgaccg ctcgcggact gcgatcaatt catcgctcgc cggaccggcg
     5161 ttcagatcgc cgcacaggag gcgaatggat gccggcccat gccaccacga ttcggcgagc
     5221 accgcggtca gctgaacacg gcggacctgc tgggcatcgc gaaggtgggt aaggtgcgta
     5281 ttcgcgatgc gaaggcacac gcctccctgc gacacacgag tcaagaggac cctccgctcc
     5341 ccatcacggt ccgtgcttgg caaaggccgt gtcgccacgt cggcaatggg gaggcgggag
     5401 aggagggcca agcccgacca cgtggcgacc gtccggccct gcacgacccg tcgcttccac
     5461 cgtgcaggaa cataggcgca cgccatcccg agcccacgcg ccaacacgcg ggccgtatcg
     5521 agcctgccgt cctcggaaag caatacctcc tgaagggcga tgatatccag atcgaggtcg
     5581 gacaagccgg cgaggagggc gtccagccgt tccaggtagg gaccatcgcc ctttgctgtg
     5641 ttgatcgtcg cgatcctgaa cgacgtcatc cgtgcttcga gaggaacgtc tccagatcat
     5701 tgaagtcgtc gatgcacgcc tcgatgcgct cgcggtccca atcccaccag gcgatgcgga
     5761 gcagtgtccc tgccacgtcc tcggggaagc gccgtcgaat cgggcgggcc ggaacgccgg
     5821 ccacgaccgt gtatggttcc acgtcccttg tgaccacagc gccggcgccg atcgcggcac
     5881 cagttccgac cgtcacgccg ggcagaacga tcgcgccatg cccaatccac acgtcgtggc
     5941 cgagcgcaac tgggtgatcg cgccgccagg cgaagaaatc ggcgtcgtcc gccgagccga
     6001 cgtcgtacat cacgcggcga taggtgaggt gatggctcgt cacgcgccag agggggtggt
     6061 tgccgggatt gaggcgcacg tgtgacgcga tggagcagaa cttcccgatg cgggtataga
     6121 ttgcgtggca actgtccatg aggtaggaat agtcacctac ctccgactcg atgatcgagc
     6181 agccctccgc aatcatcgtc caagcgccca accgagattc ctggacgacg gtcgtcggat
     6241 cgacgagggg cgctggcccg agcttcatct tcgttcgcaa tccggacgtc gcagccatga
     6301 cgccatcctg agcggggccc gtcaggagac gatgatcgcg ctgtaaagga tgcgatcgcg
     6361 tggcgtccac aaggccgcat ccgtcaggcg ccggagcggc tggtgtactc ctccagggcc
     6421 gcgttcgcct cccgttccgc ctggctcagc gcatcctccg gcgatacatc ctcgagcatc
     6481 attcgctcca gcgcttgaac gacggcgtcg cggacttccc ggtacgcgcc catcacgggg
     6541 ccggcactcg ccgcattcgt ctcgccattc agcagctggt cgtaggcgat gcgaaactgc
     6601 ggccgctcag tccagagttg gcgcacgatg tccgtttcga cggctgactt gcgaatggga
     6661 atgtacccgg tcccttcgtg ccagcgtgct tgctgctcag gctcgttgag ccacttgacg
     6721 taggtccatg ccgcctcctg ctcctccggg ctggaccgat tgacgatcca cagggacgcg
     6781 ccgccgacca ggatgccgcc gtccccctcg atgcccggca tcggcccgac gccgacctcg
     6841 atgtcgccgg cggggaactc cccgctctcg agaacctgga agatcgagcc gagcgccgcc
     6901 gaggtcgcga tcgtcatcgc cgcctcgccg gttcccacgg ccaggagatg ttccgtgccc
     6961 atcgggttgc ggcccacgtt gagcgccagg ccggcgtcga ccatctcgtc gagccaagtg
     7021 aagagctcgc gccccaaatc gtcattgaac tcgactgccg tcgccgggcc gctccgccca
     7081 ttctccgcat cgatgtacag gtgtccggcc ttgccgaacc actgctcgat ataccagcct
     7141 gacagctcga tggcgatgcc atgttgcgcc gcgcccgagt cacgaatcgc ctccgagtag
     7201 gagcgcac
//
LOCUS       DATMBB010000108         4850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_62153, whole genome shotgun sequence.
ACCESSION   DATMBB010000108 DATMBB010000000
VERSION     DATMBB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62153"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VNL92_02325"
     CDS             complement(<1..250)
                     /locus_tag="VNL92_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWO93575.1"
                     /translation="MGLEDNYWTTTFRRRLSRRHALKVAGVGGAGLATAAVVGCGDDD
                     DDDDDEGTSAATGTAPSGGTAAAEGEAKPGGTVRAALSN"
     gene            complement(327..1232)
                     /locus_tag="VNL92_02330"
     CDS             complement(327..1232)
                     /locus_tag="VNL92_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93576.1"
                     /translation="MATDELGILVARERQRPRWATWSREVWKFVRTKPLGAIGGFFVL
                     ALLIMAAFAPLIAPYHYDDQVYTETLQGPSLDHFFGTDDLGRDLFSRVVYGARISVIV
                     GFGSVAIAMAFATLIGMLSGYYGGVIDTAAQRLVEIAQAFPALILLISIIAIIGQGTG
                     QITLALGFLFAVGASRVIRGAVLGIKNNVYFEAARCVGASDVRIMLRYVLPNIAATII
                     VLATVYLGAIILVEASLSFLGYGIPPPYPSWGRMLNGIARSYIIDNPWMGLWPGLFIS
                     LAVFGFNMLGDALRDVLDPRLRGTS"
     gene            complement(1243..2193)
                     /locus_tag="VNL92_02335"
     CDS             complement(1243..2193)
                     /locus_tag="VNL92_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93577.1"
                     /translation="MGRYILRRMLLMIPTLIGISIVVFMMVRFIPGDVVDTILGEYGA
                     ADDELREQLRDQYQLNDSVVTQYFEWLGDVVQGDLGRSIISNREVLEEIRHRSVPSIE
                     LAIITVILGLLIAIPIGIISAARQDSVLDYVARSSAIGFLAIPGFWLGTLVVTLPSRW
                     WGWSPPLEYHDFTEDPVENIYILIIPAAILALSFAGTVMRLMRAQMLEVLHQDYIRTA
                     WAKGLRERSVIVRHALKNALIPVVTVIGLQIPVLIGGVVIVETIFGIPGIGSYLYQSI
                     ISRDYPIVQGVNLLVAVVVLLSNLAVDVTYAYLDPRIRYS"
     gene            2417..3385
                     /gene="pfkA"
                     /locus_tag="VNL92_02340"
     CDS             2417..3385
                     /gene="pfkA"
                     /locus_tag="VNL92_02340"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWO93578.1"
                     /translation="MRSIGLLTSGGDSPGMNATIRAVVRTAIRHGRRAVGILNGYSGL
                     LAGQFEEMDVRSVGNIIQRGGTILGTSRCPEFHEVAGRARGIEQLRKHGIDALVVIGG
                     EGSFKGAVKLQEESGIPIVGAAGTIDNDIYGTDYSIGFDTAVNTALDAIDRLRDTAES
                     HGRVFFVEVMGRHAGFIALEVGLAGGAVDILVPEYPDDMDRLANHLLERRSRGRQSSI
                     VVVSEGDELGGVFATAQEVSKRITLDYRVAVLGHVQRGGAPTARDRVLGTRMGAAAVR
                     ALIEGQHGCMAGEVGGKLVLTPLQDTWQRVKPLDADLYSLMHELSG"
     gene            3403..4785
                     /locus_tag="VNL92_02345"
     CDS             3403..4785
                     /locus_tag="VNL92_02345"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWO93579.1"
                     /translation="MHVYGNIREMYEDLAGIAEVAGPRVRVLDAEGLRGRADRLAAAA
                     AFGSAEVRSAAAWLLRAAAADLGAWAASIHPLYMARARGEWGGHVVPALNMRFGYPQI
                     RAAMRAALDLEVGLVTFEIARSEMGYTETRPQEYASGVFAAAIREGWTGPLFIQGDHF
                     QVDAKRFATEPEPELADIEALIDEAIAAGFYNIDLDSSTLVDLSRPTVDEQQRPNYEV
                     AVRLAGRVRGSQPEGLVISIGGEIGEVGKQNSTVEELDAYVTGFERLAAERDLRPGLS
                     KVSVQTGTSHGGIVLPDGSVAPVNIDFETLRELSHVARERYGMAGAVQHGASTLPSEL
                     FDRFPPLEVAEIHLATGYQNVTFESEHFPSDLRERMYAWVRQQFPGEPGREYTTDAQL
                     LYKERKRAIGPFKRELWDVPDERWEGIRADLRSILTTHFTKLGVAGTRELVARHLPRL
                     DVPIAPPFAA"
BASE COUNT          919 a         1579 c         1539 g          813 t
ORIGIN      
        1 cgttggacag cgcggcgcgc accgtgccgc cgggcttcgc ctcgccctct gccgctgccg
       61 tccctcccga tggcgccgtt cccgttgcgg cggaggttcc ctcgtcgtca tcatcgtcgt
      121 cgtcatcgcc acagcccacc acagcggccg tggcgaggcc cgctccgcca acgcccgcaa
      181 ccttcagggc gtgccggcgc gagagacgcc ggcggaacgt cgtcgtccag taattgtcct
      241 cgagtcccat gacttcaccc ccctcgagcg gagcctcttc cctccgccgc tacgtcgatc
      301 tccatcgact cctcatggcc gcctggctag ctcgttcctc gcagacgcgg gtcgagcacg
      361 tcgcgtagtg catcgcctag catgttgaac ccaaagaccg cgaggctgat gaaaagtccg
      421 ggccacaagc ccatccatgg attatcgatg atgtacgacc gcgcgatgcc attcagcatc
      481 cgtccccagg aggggtacgg cggtgggatg ccatagccga ggaagctgag cgacgcctcg
      541 acgaggatga tggcgcccag gtacaccgtg gcgaggacga tgatggtcgc ggcgatgttc
      601 ggaagaacgt accgcagcat gatccgaacg tcactcgcgc caacgcaccg cgctgcctcg
      661 aagtagacgt tgttcttgat cccgagcacg gcgccgcgta tcacgcgcga ggcgcccacg
      721 gcaaacaaga agccgagcgc cagcgtaatc tgccccgtac cctgtccaat gatcgcgatg
      781 atggagatga gcaagatgag cgcgggaaac gcctgtgcga tctcgacgag ccgctgcgcc
      841 gccgtgtcga tgactccgcc atagtagccg gacagcatgc cgatgagcgt cgcgaaggcc
      901 atcgcgatgg ccactgaccc aaagcctacg atgacggata tgcgagctcc gtaaacgacg
      961 cggctgaaca gatcgcgtcc cagatcatcg gtgccgaaga agtggtcgag cgatggtcct
     1021 tggagggtct ccgtatacac ctggtcgtcg taatgatacg gagcaatgag cggcgcaaag
     1081 gccgccatga tgagcagggc gagcacgaaa aagccgccga tggcgccgag gggcttggtg
     1141 cgaacgaact tccacacctc gcgcgaccat gtagcccagc gaggtcgctg ccgctccctg
     1201 gcgacgagaa tgccgagctc gtcggtcgcc acttctcccc ccttacgagt acctgatccg
     1261 cggatcgaga taggcgtatg tcacgtcgac ggccaggtta gacaacaaaa ccaccaccgc
     1321 gacgagcaga ttcacaccct gcacgatcgg gtagtcgcgt gagatgatcg actgatacag
     1381 gtacgatccg atgccgggga tgccgaagat ggtctccaca atcacgacgc caccgatgag
     1441 caccggaatc tgcaaaccga tgacggtaac gacagggatc agcgcgttct tcaacgcgtg
     1501 ccggacgatg accgagcgct ctcggagtcc cttagcccat gccgtccgga tgtagtcctg
     1561 gtgcagaacc tccagcattt gcgcccgcat gaggcgcatc accgtccccg cgaacgagag
     1621 cgccaggatg gccgccggga tgatgagaat gtagatattc tctactgggt cctccgtaaa
     1681 gtcgtgatac tccaaaggcg ggctccaccc ccaccaccgg ctcggtaacg tgaccacgag
     1741 cgtgcccagc cagaagcctg ggatggcgag gaagccgatg gccgagctcc tcgccacgta
     1801 gtcgaggacg ctgtcctgac gggcagcaga gatgatgccg atcggtatgg cgatgaggag
     1861 gccgaggatg accgtgatga tcgcgagctc gatgctgggc accgagcggt ggcgtatctc
     1921 ctccagcacc tcgcggttgc tgatgatcga gcggcccagg tctccctgaa cgacgtcgcc
     1981 gagccattcg aagtactgcg tgacgaccga atcgttgagc tggtattggt cgcggagctg
     2041 ctcgcgcagc tcatcgtcgg ccgccccata ttcgccgaga atagtatcga cgacatctcc
     2101 tggaatgaac cgcaccatca tgaacacgac gatgctgatc ccgatgagcg tcggaatcat
     2161 gagcagcatt cgtctcagga tgtatcgacc catatcctcg atgtgggcag gccgccccca
     2221 ctccggggct gagggcaaat ctcgacgtat cgccctcggt ctgctcaggt tcgcgcaatc
     2281 ataggcccat gcgctagcct tgtccagagc tatgtaaact gcgatacagt cacaacagaa
     2341 gagtgttgtt cgggcccgct gcccgagcta ggctggaagg gtcgcgctag cctgtgagta
     2401 cgagatgaac gacaacattc gaagtattgg tctcctgacc agcggcgggg actcccccgg
     2461 gatgaacgcg accattcggg ccgtcgttcg caccgccatt cggcatggcc gacgagcggt
     2521 cggcattctg aacgggtaca gcggcctcct cgccggccaa ttcgaggaga tggacgtccg
     2581 ctctgtcggc aacatcattc agcgaggggg aacgatcctc ggtacctctc gctgtcccga
     2641 gttccacgag gttgcgggcc gggcgcgcgg catcgagcag ctccgaaaac atggcatcga
     2701 cgcccttgtc gtgatcggtg gggagggttc gttcaagggc gccgtgaagc tccaggagga
     2761 aagcggcatc cccatcgtcg gcgcagcagg gacgatcgac aacgatatct acgggaccga
     2821 ttactccatc gggttcgata cggccgtgaa cactgcgctc gacgccatcg accgccttcg
     2881 agatacggcc gaatcgcacg gccgcgtctt cttcgttgag gtgatgggcc ggcacgccgg
     2941 cttcatcgcc ctcgaggtcg ggctcgccgg gggcgccgtc gacatcctgg tgcccgagta
     3001 ccccgacgat atggaccggc tggccaatca tcttctcgaa cgtcgaagcc gaggccgcca
     3061 gtcatccatc gtcgtggttt cggagggcga cgagctgggc ggcgttttcg caacggcgca
     3121 agaggtatcg aagcgtatca cgctcgacta tcgagtcgcc gttcttggcc atgtccagcg
     3181 cggaggcgcg ccgaccgccc gcgaccgggt gctgggcacg cgcatggggg cggcggccgt
     3241 cagggcgctc atcgagggac aacacggctg catggccggc gaggtcggtg gcaagcttgt
     3301 gctgacgcca ctgcaagata cgtggcagcg tgtcaaaccg ttggatgccg acctctactc
     3361 cctcatgcac gagctctcag gataggtaga gggaagtcgc cgatgcacgt gtacggcaac
     3421 atacgcgaga tgtacgagga tctggctggc atcgccgagg tggcgggccc ccgcgtacga
     3481 gttctcgatg cggagggcct acgcggccga gcggaccggc ttgccgcagc cgccgccttc
     3541 ggatcggccg aggtccgctc cgccgcggcc tggctgctgc gagcggccgc cgccgacctg
     3601 ggagcttggg ctgcctcgat ccatccactg tacatggcgc gcgcccgcgg agagtggggt
     3661 gggcacgtcg tgcctgccct caacatgcgc ttcggctacc cccagatacg cgccgccatg
     3721 cgcgccgccc tcgacctcga agtcggcctt gtgaccttcg agatagcgcg atccgagatg
     3781 ggctacacgg agacgcggcc gcaggagtac gcctccggcg tgttcgcggc ggccattcgc
     3841 gagggatgga cgggcccgct cttcattcag ggagaccact ttcaagtgga tgcgaagcga
     3901 ttcgcgacgg aacccgagcc cgagctcgcc gacatcgaag cgttgatcga cgaagcgatc
     3961 gcggccggct tctacaacat cgacctcgac tcttcgacct tggtcgatct gtcacgcccc
     4021 acggtcgatg agcagcagcg gccgaactac gaggtggccg tccgtctcgc ggggcgcgtg
     4081 cgaggttcgc agcccgaagg tctcgtcatc tccatcgggg gcgaaatcgg cgaggtcggc
     4141 aagcagaata gcaccgtcga ggagctcgat gcgtacgtca cgggcttcga gcgcctcgcc
     4201 gccgagcgcg acctccgtcc aggcctgagc aaggtcagcg tccaaaccgg cacgagtcac
     4261 ggcgggatcg tcctccccga cggcagcgta gcccccgtga acatcgactt cgagaccctc
     4321 cgggagctct cgcacgtggc gagggaacgt tacgggatgg cgggcgcggt ccagcatggg
     4381 gcctcaaccc tccccagcga actctttgac aggttcccgc ctctggaggt ggccgagatt
     4441 cacctggcca ccggttacca gaacgtcacg ttcgaaagcg agcatttccc cagcgacttg
     4501 cgcgagcgca tgtacgcctg ggtacggcag cagtttcccg gcgagccggg ccgcgagtac
     4561 acgaccgatg cgcagctcct gtacaaggag cgcaaacgcg cgatcggccc attcaagcgg
     4621 gagctctggg acgtacccga cgaacggtgg gagggcatcc gtgccgacct gcgcagcatc
     4681 ctgacgacgc acttcaccaa gctcggcgtc gcggggacgc gtgagctcgt ggcgcggcat
     4741 ctgcctcggc tcgacgtccc gatcgcgcca cccttcgctg cataagcacc acttacatcg
     4801 tcacacgtac ggtttgtacg gctccgcgac acgcccgcac ctcgccccta
//
LOCUS       DATMBB010000109         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_545196, whole genome shotgun sequence.
ACCESSION   DATMBB010000109 DATMBB010000000
VERSION     DATMBB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545196"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VNL92_02350"
     CDS             complement(<1..751)
                     /locus_tag="VNL92_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWO93580.1"
                     /translation="MYDSPSHVLVTGGAGFIGSAFVGMLLRDPGGPRVTVLDKLTYAG
                     NPANLKPFEGDPRFRFLRGDIAETDDVKAALDGVDVVVNFAAETHVDRSLLDAFEFIR
                     TDVEGTVVLLEASRQAGVSRFVQISTDEVYGPIAPGESADEAWPLRPRSPYAASKAGG
                     DLQCLAFHASYDFPVIITRGSNTYGPRQYPEKVVPLFTINAILGRALPLYGDGRQVRD
                     WMHVEDHCAGIWLALTRGEPGTVYNIGPGDER"
     gene            complement(860..1945)
                     /locus_tag="VNL92_02355"
     CDS             complement(860..1945)
                     /locus_tag="VNL92_02355"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HWO93581.1"
                     /translation="MANGSALKGLILSGGKGSRLRPFTYTGAKQLVPIANRPVLFYAI
                     QHLVDAGITDIGIVVGETGDQVRTAVGDGSAFGARVTYIEQPKPLGIAHGIIVAREFL
                     AGDPFVLFLGDNFLRGGIAPLVERFRDDNAASYVLLCPVPNPREFGIAELADDGRLLR
                     VVEKPSDPVSNLAVIGIYLFDSRVHEAVSAIRPSARGELEITDTIQYLLDAGMPVRAH
                     LVEGSWIDTGKMDDILQANRLMLDDVEPRIDGSVDEASSVQGKVLIGSGASIVNSVVR
                     GPAVIGNDTRISNSYIGPFTCIDHGCIIEDSEISGSVVMEHTSIRAIGHRIEDSLIGR
                     YVELAGGRLKPRGYRLVLGDHSKVEGP"
     gene            complement(2063..2557)
                     /locus_tag="VNL92_02360"
     CDS             complement(2063..2557)
                     /locus_tag="VNL92_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWO93582.1"
                     /translation="MTWLDIVILVLVALATLQGRMQGAVRAFITLAGVLVAALVAGIF
                     YDDLADSLSPIITNDHDAIVVGFLSIFFATYLGSELLAGLSTGVVSLLLLGPWTRPAG
                     MGLGALKALLLVDAFLIFLVTYPSLGMRGVVDDSALAPFFVDDFPIMRFLLPGEFDAA
                     AAAF"
     gene            complement(2677..>2912)
                     /locus_tag="VNL92_02365"
     CDS             complement(2677..>2912)
                     /locus_tag="VNL92_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWO93583.1"
                     /translation="WVLAGDVIPHQHIRLLARTACVLIVALMGPARVFTGAHWPSDVV
                     GGYAFGLLVLAVIVWMYRTLRRPSRQLAMTEAG"
BASE COUNT          563 a          922 c          967 g          460 t
ORIGIN      
        1 cacgttcgtc gccgggaccg atgttgtaga ccgtgccggg ctcgccgcgc gtgagcgcca
       61 gccagatgcc agcgcagtgg tcctccacgt gcatccagtc cctaacctgc cggccatcgc
      121 cgtagagtgg cagggcgcgc ccgaggatgg cgttgatggt gaagagcggc acgaccttct
      181 ccggatactg gcgagggccg tacgtattgc tgccgcgcgt gatgatgacc gggaagtcgt
      241 agctggcgtg gaaggcgagg cactgcaaat cgcccccggc cttgctggcc gcgtaggggc
      301 tgcggggtcg cagcggccag gcctcatcgg cgctttcgcc tggagcgatg gggccataca
      361 cctcgtcggt ggagatctgt acgaagcgac tcacgcctgc ctgccgcgac gcctccagca
      421 gcacgacggt cccctcgaca tcggtgcgta tgaactcgaa cgcgtccagc agcgagcgat
      481 cgacgtgcgt ctccgcagca aagttgacga cgacatccac tccatcgagc gcagccttca
      541 catcatcggt ctcggcgatg tccccacgga gaaagcggaa tcgaggatcg ccctcgaagg
      601 gcttcaggtt cgccgggttc ccggcgtagg tcagcttgtc gagcacggta acgcggggcc
      661 cgcccgggtc gcgcagcagc ataccgacga aggcgctgcc gatgaagccg gccccgccgg
      721 taacgagcac atgcgaggga ctgtcgtaca tacgagcgta gtgtagcagg accagagcga
      781 gcgttcgagg gcgacccgcc tgggctcaac gccgcccctg cggcctcagg atggtcgatc
      841 aggcaggtgc gggacggccc tacggaccct caaccttcga atggtcgccc aggacaagcc
      901 ggtagccgcg cggtttcagc cgcccgcctg ccagctccac gtagcggccg atgaggctgt
      961 cctcgattcg atggccaatg gctcgaatgc tggtgtgctc catcacgacg ctgccgctga
     1021 tctcgctatc ctcgatgatg cagccgtgat cgatgcaggt gaacggccca atgtagctgt
     1081 tcgaaatgcg cgtatcattg ccgatgacgg ccggcccccg caccaccgag ttgacgatgg
     1141 atgcgccgct cccgatgagg accttgccct gcacgctgga ggcctcgtcg acgctgccat
     1201 cgatgcgtgg ctcgacatca tcgagcatga gccggttcgc ctgcaggatg tcgtccatct
     1261 tgccggtgtc gatccagctc ccttcgacga gatgggcgcg cacgggcatg ccggcatcga
     1321 gcaaatactg gatggtatcc gtgatctcga gctcgccccg agccgatgga cggatggcgc
     1381 tgacagcctc gtggacgcgc gaatcgaaca gatagatgcc gatgacggcc aagttggaga
     1441 cggggtcgga cggcttttcg acgacgcgca ggaggcggcc gtcgtccgcc agctcagcaa
     1501 tgccaaactc gcgcgggttc gggacagggc agaggagcac gtacgacgcc gcattgtcgt
     1561 cgcgaaaccg ctctaccagg ggagcgatgc caccacgcag gaagttgtcg ccgaggaaga
     1621 gcacgaacgg atcgcccgcc aagaattcgc gagcgacgat gatgccgtga gcgataccga
     1681 gcggctttgg ctgctcgatg tacgtgaccc tcgcgccgaa tgcggagcca tcaccaacgg
     1741 cagtccgcac ctgatcgcca gtctccccga cgacgatccc gatatccgtg atgccggcat
     1801 cgaccaaatg ttggatggca tagaagagca cgggcctgtt cgctatcgga acaagctgct
     1861 tggcgcccgt gtaggtgaaa gggcgaaggc ggctcccctt gcctccggaa aggatgagtc
     1921 cctttagggc cgatccgttg gccacgctca cctcgctcag gtgcccgccc aaccctgcgc
     1981 gaggagcgcg tggctggctt gccgaaggcg attgtaggtg tcgcgaaatc tcagtgtcaa
     2041 ccgagagtac tcgcggctcc ggtcagaatg ctgctgctgc tgcgtcgaac tcgccgggga
     2101 gcaggaagcg catgatgggg aagtcgtcga cgaagaacgg cgcgagcgcc gagtcgtcca
     2161 ccacgccgcg catcccgagc gatgggtagg tcacgagaaa gatgaggaaa gcatcgacca
     2221 ggagcagcgc cttcaatgcg cccaatccca tgcccgcggg gcgcgtccac gggcctagga
     2281 gcagcaacga gaccactccg gtcgacagcc cggcgagcag ctcgctgccg agatacgttg
     2341 cgaagaagat ggacaggaac ccaacgacga tcgcgtcgtg gtcgttcgtg atgatgggag
     2401 agaggctgtc cgcaagatcg tcgtagaaga tgcctgccac cagagccgcg acgaggacgc
     2461 cagctaaggt gatgaaggct cggacagcgc cctgcatcct gccctgcaac gtggccaggg
     2521 ccacgagaac caggatcacg atgtcgagcc atgtcatccc gcgcgcagat ggttacgggc
     2581 gactcgggac catgatggac gttcggatgg ctgactgcgg cccatgtcta ctccggcgcg
     2641 cgaaggtgcc cggagtgtag cacaccgcat tcgcgatcag cctgcttcgg tcatagccag
     2701 ctgtcgcgat ggcctgcgca gcgtgcggta catccacacg atgacggcaa gcacgagcag
     2761 gccgaacgcg tagcccccca ccacgtcgct tggccagtgc gcccccgtga agacgcgcgc
     2821 cgggcccatg agcgcgacaa tcaggacaca cgctgtgcga gccaggagac ggatgtgctg
     2881 gtgggggatg acgtcgccgg cgagcaccca ga
//
LOCUS       DATMBB010000110         2628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_459431, whole genome shotgun sequence.
ACCESSION   DATMBB010000110 DATMBB010000000
VERSION     DATMBB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459431"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VNL92_02370"
     CDS             <1..336
                     /locus_tag="VNL92_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007140333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWO93584.1"
                     /translation="RALLDCTGGWYTVQDWSGIRMQTLLQRAGIRDGARSIVVRSHTG
                     YERRFDLDDAGTLLLATHVTGDVLSRGHGYPMRLVAPGSRGFDWVKWVTDVEVSDAHP
                     LWEPPLPLQ"
     gene            complement(356..964)
                     /locus_tag="VNL92_02375"
     CDS             complement(356..964)
                     /locus_tag="VNL92_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWO93585.1"
                     /translation="MTRFDIFGRRQKEEREKFGDRLPPGQKVTEGWPVLHYGGIPKID
                     LTTWRFRVFGAVEEEKEWTWEEFTALPHMTSRSDIHCVTQWSKFDNDWEGIGWRELMK
                     HIAVKPEAKHVMVHCYGGYTTNVPLADLDDDDTLFAVKHNGEPLAPEHGWPMRLVVPK
                     LYFWKSAKWVRGLEFIAEERPGFWEMYGYHIHGDPWKEERYS"
     gene            1255..2319
                     /locus_tag="VNL92_02380"
     CDS             1255..2319
                     /locus_tag="VNL92_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWO93586.1"
                     /translation="MATYRQDVIDRLKADGMDLSWLDAPSPKGARAEPGKKGLTLEAA
                     AVDGYNEVPRTTDNLGSAIRGAAERPEVGPIMLRWGNKAVAWSESVELLYEEALQRQW
                     SSARDIPWDQLTELPDDVELAMCQVATFFNQVEFIAGDQPGRWVQNLSPDHFEVGLFL
                     STQVMDEARHLDVFRKRALANGGKLMLGNEPASGALAQWDFSQMTAQLHVLGEGFVQT
                     MFRMGEWYSFNEAEKKIYRLAAQDESRHVAFGVMHLKYLLETEPERREQIHSYFDMAE
                     TGLGAGNQAGVLTGSGPTAESLAVLLGAGAKNIDEGWRRLFAINKRQVQEYVHRLDVI
                     GMSDRLDRMNPRLKAFIGVN"
     gene            2378..>2628
                     /locus_tag="VNL92_02385"
     CDS             2378..>2628
                     /locus_tag="VNL92_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93587.1"
                     /translation="MSEVEGVPDELRDHVERYRWPKATVAALVARGVDPDRILSAITS
                     GTPVSHFLAQVNAELPAEAPKIDLTWMDVPTERLQKIKP"
BASE COUNT          469 a          818 c          866 g          475 t
ORIGIN      
        1 cgcgccctgc tcgattgcac gggcggatgg tacacggtgc aggactggtc gggcatccgc
       61 atgcaaacgc tgttgcagcg agccggcatt cgcgatggcg cccggtcgat cgtcgttcgc
      121 tcgcatactg gctacgaacg ccgtttcgac ctcgacgatg ccggtacgct actcctcgcg
      181 acgcacgtca ccggggacgt gctatcgcgt gggcacggct acccgatgcg cctcgttgcg
      241 cccggctcgc gcggattcga ctgggtgaag tgggtgaccg acgtcgaggt gagcgacgct
      301 catccgctct gggagccgcc tctgccgctt cagtagcctc gccgaaatgc cccggctagg
      361 agtagcgctc ctccttccac gggtctccgt ggatgtggta gccgtacatt tcccagaatc
      421 cgggccgctc ctcggcgata aactccagcc cgcgcaccca cttggcgctc ttccagaagt
      481 agagcttcgg cacgacgagg cgcatcggcc agccatgctc gggcgccagc ggctccccgt
      541 tgtgcttgac ggcgaagagc gtgtcgtcgt catcgaggtc ggcgagcggt acgttggtgg
      601 tgtacccgcc gtagcagtgc accataacgt gcttcgcctc gggctttaca gcgatgtgct
      661 tcatcagctc ccgccagccg atgccctccc aatcgttatc gaacttggac cattgggtaa
      721 cgcagtggat gtcggagcgg ctggtcatgt gcgggagggc cgtgaactcc tcccacgtcc
      781 actccttttc ctcctccact gcgccgaaca cgcggaatcg ccacgtggtc aggtcgatct
      841 tcgggatgcc gccgtagtgg agcacgggcc agccctcggt gaccttctgc cccggcggca
      901 gacgatcgcc gaacttctcg cgctcttcct tctgtctccg tccaaagatg tcgaagcggg
      961 tcatgccttg gctcgctttc cgatgccacg cttgatggcg ctcatcgtgc tcggcattgt
     1021 aggaaggcta ctccggtcgg tcaagcagca gcgccgagac gcgcccaggc agtcatgggt
     1081 attcgcccgg cccatgtacg acctccaatt cccagcggga tgcccaacca aacgtacgat
     1141 cggcaaagag ttagccgtag gccttgacac cggtagagtt cgccgcctag tgtcaggcat
     1201 cgctcgattt cggggtagtc cgccgtgggc ggcaagtgaa gtgaggggag aagcatggct
     1261 acgtatcgtc aggatgtcat cgaccggctc aaggccgacg gcatggacct ttcctggctc
     1321 gatgcgccga gccccaaggg cgcgcgcgct gagcccggta agaagggatt gacgctcgaa
     1381 gcggcggcag tcgacggata caacgaggtg ccccggacga cggacaacct gggctcggcc
     1441 atccgtggcg ccgcggagcg ccccgaggtg gggccgatca tgcttcgctg gggcaacaag
     1501 gccgtggcct ggtcggaaag cgttgagctg ctctacgaag aggccttgca gcgccaatgg
     1561 tcgtccgcgc gggacatccc gtgggatcag ctcaccgagc tgcccgacga cgtcgagctt
     1621 gccatgtgcc aggtggcgac attcttcaat caggtcgagt tcatcgcggg cgaccagccg
     1681 ggccggtggg tgcagaacct gagtccggac cacttcgaag ttggcctgtt cctcagtacg
     1741 caggttatgg atgaggcgcg ccacctcgac gtgttccgca agcgcgcgct ggcgaacggc
     1801 ggcaagctca tgctcggaaa cgagcccgcg tctggtgcgc tggcccagtg ggacttctcg
     1861 cagatgacgg cgcagttgca cgtcctcggc gagggcttcg tgcagacgat gttccgcatg
     1921 ggggagtggt actccttcaa cgaggcggag aagaagatct accgattggc cgctcaggac
     1981 gagagccgcc acgttgcctt cggcgtcatg cacctgaagt acctgctcga aacggagcca
     2041 gagcgccggg agcagatcca ctcctacttc gacatggccg aaaccggcct gggagctggg
     2101 aaccaggccg gtgtgctcac cgggtcggga cccacggccg aatcgctggc tgtgctgctc
     2161 ggcgccggag cgaagaacat cgatgagggc tggaggcgcc tgttcgctat caacaagcgg
     2221 caagtacagg agtacgtgca ccggctcgat gtgatcggca tgagtgaccg cctcgaccga
     2281 atgaaccctc ggctgaaggc gttcatcggc gtgaactagg cgcccgtcag cgtattcagg
     2341 agtaacggcg cggcgtacgc cgcggcaagg agattcgatg tctgaggtgg aaggcgtccc
     2401 ggacgaactc cgggaccatg ttgaacgata ccgctggccg aaggcgaccg tggcagcgct
     2461 ggtagcgcga ggcgtcgatc cggaccgcat cctgtccgcg ataacgtcgg gcacgccggt
     2521 atcgcacttc ttggcacaag tgaatgcgga actgccggcc gaggctccga aaatcgattt
     2581 gacgtggatg gatgtgccca cggagcgtct tcagaagatc aagccgag
//
LOCUS       DATMBB010000111         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_578002, whole genome shotgun sequence.
ACCESSION   DATMBB010000111 DATMBB010000000
VERSION     DATMBB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578002"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VNL92_02390"
     CDS             <1..512
                     /locus_tag="VNL92_02390"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWO93588.1"
                     /translation="NPELAIQALLARGYPVFALTEPLEPPALSRFLDERRSAAGHRFR
                     AATYAALKEAMRTLRAGSNIALMFDRDIRGRSITIDLCGAPAQVPVGAVEFALRTGAP
                     IVPVFIHRRVDQRFDVWIEPALHVGNAGDREARVRETVRELFRRFEAHLRCDPGQWMV
                     LERIWDAPG"
     gene            541..1338
                     /locus_tag="VNL92_02395"
     CDS             541..1338
                     /locus_tag="VNL92_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HWO93589.1"
                     /translation="MRASIEPEAGRGHALGKADLQVHTAHGDGMAGGEPMLAYIETKT
                     DLDIVVITDHDDIGGAWKTRELWERGTYSFEVIVGIEVTTRAGHLLAIDVDRPIPSFR
                     RPEETIRLIHEAGGLAVIPHPMSWLTRSIGQRTISRITSDPDPAVAFDGIEVMNQTIA
                     ARVTRAKAQRLNRDRWRLAETGGSDAHFLQAIGTAYTTFPGHTMAELRVALRDHTTGF
                     GWSAFPSLRRLGVRQIARQQARGLMVTPRNVVFRPIGRTILRRRASA"
     gene            1341..2528
                     /locus_tag="VNL92_02400"
     CDS             1341..2528
                     /locus_tag="VNL92_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWO93590.1"
                     /translation="MAQVSPYDFSYPGGVNSHILQLTWHFEQLGHEVRILAPASDDGR
                     LLGAGRNAIIIGRPIAVPLSGSVVRIARNPRLGGQIAEVLEREQFDVIHIHEPLMPWL
                     PFHTLRLSSCVTVGTFHAASERGNLLYSYGRGILRRWFKKLDGRIAVSPAAAHHVARY
                     FPEHYDIIPNGIDVARYAAEVQPFEHLRDGKLNILFVGRLEKRKGLKYLLRAYAALKR
                     DHPNLRLIIAGEGAVRHGYEVSVRKAGLKDVIFTGYVSEEDKIRYLHTADIFCSPATG
                     NESFGIVLLEAMAAGLPIVATNIRGSQFVVTDGVEGLLVRPKNEAGLTQALGRLIEDP
                     DLRTRLANGGRERVREFDWWKLARRILSYYDRLIYEREAGAREAAASPHSYPPEASIG
                     QAG"
     gene            2533..>2906
                     /locus_tag="VNL92_02405"
     CDS             2533..>2906
                     /locus_tag="VNL92_02405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWO93591.1"
                     /translation="MRRISLRNPIPHRLPETITEPIGRTVARTGLTPNVISVIGVTGN
                     LVAGGFAGAGWFIPAGLTMVVFSGVDLLDGAVARATGRATKFGAVFDATLDRVSEAAV
                     LFGLLWHYTSEGERTESLLIYAA"
BASE COUNT          492 a          972 c          948 g          494 t
ORIGIN      
        1 gaaacccgga actcgccatt caggccctcc tcgcccgcgg ctatcctgtg ttcgcgctca
       61 ccgagcctct cgaacccccc gcactatcgc gattcctcga cgagcggcgc tcggccgcgg
      121 gccaccgctt tcgggcggcc acttacgcgg cgctgaagga agccatgcgc acgttacgcg
      181 cgggctccaa catcgcgctg atgttcgatc gggatatccg cggccggagc atcacgatcg
      241 acctctgtgg ggcacccgcg caagtacccg ttggcgccgt tgagtttgcg ctgcgaacgg
      301 gcgccccgat cgtccctgtc ttcatccatc gccgcgtcga tcagcgcttc gacgtatgga
      361 tcgagccggc gctgcacgtc gggaacgccg gcgatcgcga ggcccgcgtc cgggagacgg
      421 tacgcgaact cttccggcgg ttcgaagcgc accttcgctg cgatccgggg cagtggatgg
      481 tgctggagcg catctgggat gcgccgggct aggcgtgatg cactcggcta tactacggct
      541 atgcgagcgt ccattgagcc cgaagccggc cgcggacacg cgctcggcaa ggccgacctc
      601 caagtacata ctgcccacgg cgacggtatg gcgggcggcg aaccgatgct tgcgtacatc
      661 gagacgaaga ccgacctcga catcgtcgtc attaccgacc acgatgacat cgggggcgcc
      721 tggaagacac gtgagctctg ggagcgcggc acgtacagct tcgaggtcat cgtcggcatc
      781 gaggtgacga cgcgtgctgg gcatctgctc gccatcgatg tcgacaggcc gattccgtcg
      841 ttccgccgcc ccgaggagac tattcggctc atccacgagg ccggcgggct ggctgtcatc
      901 ccgcacccca tgagctggct aacgcggagc atcggacagc gcaccattag ccgcattacg
      961 tcggacccag accctgcggt cgccttcgac ggcatcgaag tcatgaacca gacgatcgcg
     1021 gcgcgggtga cgcgggcgaa ggcgcagcgc ctgaaccgcg ataggtggcg cctggcggag
     1081 acgggcggca gcgacgcgca cttcctccag gccataggga cggcctacac gacgtttccg
     1141 ggtcacacga tggcggaact gcgtgttgcg ctccgtgacc acacgacggg cttcggctgg
     1201 tcggcgtttc cgagcctgcg ccgcctcggc gtgcgacaga tcgcgcggca gcaggcacgg
     1261 ggcctcatgg tgacgccccg gaacgtcgtc tttcgaccca tcgggcgcac gatcttaaga
     1321 aggagggcat cggcctgaag atcgcgcaag tatcccccta cgacttctcc tatccgggag
     1381 gcgtaaactc gcacatcctg cagctcacgt ggcacttcga gcagttggga cacgaggtgc
     1441 ggatcctcgc tcctgcctcg gacgacgggc gactcttggg ggccggtcgg aacgccatca
     1501 tcatcggccg gccgattgcc gtgccgctca gcgggtcggt tgtgcgcatt gcgcgcaatc
     1561 cccggctcgg tgggcagatc gcagaggtgc tggagcggga gcaattcgac gtgatccaca
     1621 tccatgagcc gctcatgcca tggctgccgt tccatacgct ccggttgtcc tcgtgcgtca
     1681 cggtcggcac gttccacgct gcgagcgaac ggggaaacct gctctactcc tatgggaggg
     1741 gcatcctgcg gcgttggttc aagaagctcg acggaaggat cgccgtctcc ccagctgccg
     1801 cgcaccatgt ggcgcgctac tttccggagc actacgacat catccccaac ggcatcgacg
     1861 tggcgcgcta tgcggcggag gtgcagccgt tcgagcacct gagggatgga aagctcaaca
     1921 ttctgttcgt cggccggctc gagaagcgga aggggctgaa gtacctgctc cgggcctatg
     1981 ccgcgctgaa gcgcgaccac ccgaacctgc gcctcatcat cgccggcgaa ggcgccgtac
     2041 gacacggcta cgaggtatcg gtccgaaagg ccggcctgaa ggacgtcatc ttcaccggct
     2101 acgtgagcga ggaagacaag atccgctacc tgcatacggc cgacatcttc tgctcgccgg
     2161 cgaccggtaa cgagagcttc gggatcgtgc tcctcgaagc gatggccgcg ggcctcccca
     2221 tcgtggccac caacatccgg ggttcgcagt tcgtcgtcac ggacggcgtc gagggtctcc
     2281 tcgtgcgccc caagaacgaa gccggcctca cccaagccct gggacgactg atcgaggatc
     2341 cggacctgcg aacgaggcta gcgaatggcg ggcgcgagcg cgtacgggag ttcgattggt
     2401 ggaagctggc tcggcgcatt ctgtcctact acgatcgcct catctatgaa cgggaggccg
     2461 gggcccggga ggccgccgcc tcgccccact cctaccctcc cgaagcctcc ataggtcagg
     2521 cgggctgaac gcgtgcgccg catttcgttg cgcaacccca tcccccaccg cctccccgaa
     2581 acgattacgg agcccattgg ccgcaccgtc gcccgcacgg gcctcactcc caacgtcatc
     2641 tcggtcatcg gggtcaccgg caacctggtt gcgggggggt tcgccggggc cggttggttc
     2701 attccggccg gcctcacgat ggtcgttttt agcggcgtcg acctgctcga tggagcggtt
     2761 gcccgagcca cggggcgagc aactaagttc ggcgccgtgt tcgacgcgac gctcgatcgc
     2821 gtttcggaag cggcggtgct gttcggcctg ctgtggcatt atacgagcga gggcgagcga
     2881 accgagagcc tgctgatcta tgccgc
//
LOCUS       DATMBB010000112         7876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_503473, whole genome shotgun sequence.
ACCESSION   DATMBB010000112 DATMBB010000000
VERSION     DATMBB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7876
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503473"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VNL92_02410"
     CDS             <1..182
                     /locus_tag="VNL92_02410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWO93592.1"
                     /translation="FLDDVPVDKVSAFEAALLGFMESNHPEIGRAIEEQKTLTDELRE
                     QLIAAIREFKQTMPY"
     gene            214..1113
                     /gene="atpG"
                     /locus_tag="VNL92_02415"
     CDS             214..1113
                     /gene="atpG"
                     /locus_tag="VNL92_02415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HWO93593.1"
                     /translation="MANIRIIRRRIRSVQSTAKITKAMELVAASKMRRAQERALAGRP
                     YAEQLREVLSHLAEQTGGDIDPTGATVLHPLLVQRSPVRRALIIEITTDRGLCGGLNA
                     NLNRRTAALALELRGQGIEVSLVNVGRKGREFFGRTGYPIEAEFANLPDYPAMADTLA
                     ISHVAVTDFLEGRADRVLIVYPRFINTTIQRPEVLQLLPVQPPETTETITAKYADYIF
                     EPSPEEVLETLVPRYVETQLYVTVLEKAASEQSARMVAMRNASDAAADMIQDLTLTYN
                     KTRQEQITKELLDVVGGAQALTR"
     gene            1113..1781
                     /locus_tag="VNL92_02420"
     CDS             1113..1781
                     /locus_tag="VNL92_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWO93594.1"
                     /translation="MRERFEALVFDMDGVLVDSEPLHLEATRSILGAEGIEISWEVFK
                     EYVGTTVEATWRDMMLRYGLGGSYEDYLQRYEEAILGVLARELTPARGAPELIEAARE
                     RGKRLGLASSARGTWIAATLAGLGLTDVFDVIASGDMVTHGKPAPDIYLLAAQRLGIA
                     PKRCIAIEDAPKGVASAKTAGMYTVGVLTDYNDAASMAAADELVNSLEEFNYDLLAAF
                     EGAA"
     gene            1804..3204
                     /gene="atpD"
                     /locus_tag="VNL92_02425"
     CDS             1804..3204
                     /gene="atpD"
                     /locus_tag="VNL92_02425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWO93595.1"
                     /translation="MATGKVTQVIGTVVDAEFPPDSLPDLYNAVEIQMDGGTLVAEVQ
                     QHLGNNWVRCLAMDTTDGLRRGVDVFDTGRPISVPVGPSTLGRLFNVLGEPLDNLGAV
                     QTEASWPIHRMPPSFEQQETQAQTLETGIKVVDLIAPLARGGKVGLFGGAGVGKTVLI
                     QELIRNIAAEHGGYSVFAGVGERSREGNDLWHEMQESGVIDKTVLVFGQMNEPPGVRL
                     RIALTGLTMAEYFRDEEGRDVLLFIDNIYRYTLAGMEVSALLGRMPSAVGYQPTLATE
                     MGDLEERITSTNKGSITSFQAIYVPADDYTDPGVSTTFAHLDSTVALDRALTEQGLYP
                     AMDPLSSSSRILDPRVVGQEHYDVARGVQQVLQRYKDLQDIIAILGVDELSEEDKLAV
                     ARARRIQRYLTQPFFVAEGFTGQPGRFVPIRETVRGFREILNGEHDDLPEQAFYMVGT
                     IDEAVEKGRQMATAAG"
     gene            3209..3664
                     /locus_tag="VNL92_02430"
     CDS             3209..3664
                     /locus_tag="VNL92_02430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWO93596.1"
                     /translation="MPLTVEVITAERRVLSEAGVDEVIVPGAMGELAILPRHAALITA
                     LEPGEMRLVRGGDETSFVLTGGFLEVKDDKVTVLADAAERAEEIDVARAEEARRRAQE
                     RLAEHVSAEEMARAEAALRRSLMRLRVAERRRRRGGSSGMGPGSTRPGS"
     gene            3778..5088
                     /gene="murA"
                     /locus_tag="VNL92_02435"
     CDS             3778..5088
                     /gene="murA"
                     /locus_tag="VNL92_02435"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWO93597.1"
                     /translation="MRGAVTTRSTSSFVIEGGTPLRGDVHVSGAKNAVLPALAATLLT
                     DEECIIENVPDIGDVGVMTAIIQSVGAEVDANDPACLRVVVPRVTQLGPPSELVVNIR
                     ASFLTMGALLGRLGEASCCFPGGDVVGQRPIDTHLAGFQMLGATVGRDGERFTARTTG
                     LRGARIFMDYPSVLGTENVMLAATLARGHTTIINAAAEPEVVFLARMLNSMGASIHGA
                     GTHTIDIEGVERLHGCHLSVIPDRIEAGTFLVAAAATGGDVVVRGAKPRDMDATLAKL
                     AEAGVTIEELPDGVRARRERELHAIHLQALPYPGLPTDMQAPMAALLTQAEGTSQIHE
                     RVFDNRLLYVDELRKMGAEIITSGGTTAIVLQRTPLVGATVRALDVRAGAALVLAGLV
                     AEGSTTILDVYHLDRGYAALDEKLRGLGARIERREGAVVEPALS"
     gene            5147..6190
                     /locus_tag="VNL92_02440"
     CDS             5147..6190
                     /locus_tag="VNL92_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWO93598.1"
                     /translation="MRFGKRIGIDLGTANVLVYEQGRGIVVNEPAVVALAHRDNTVVA
                     VGNEARAMMGRTPDSISIIRPMRDGVIADYLVTEAMLRYFIGKAIPRFSILKPEVMVC
                     IPAGVTSVEQRAVRDAAEQAGARSPAHLVPEPIAGAIGANLPIDTASGNMIVDIGGGR
                     TEAAVISMYGMVAAESVRMAGDRLDDAIVAYVKRRHNLVIGERTAEDCKRQIGCAIPV
                     DPELRMEMRGRDVITGMPRATTITSTEIANALQDNLAVMMHAIRSVLERTPPELASDV
                     IDRGIVLTGGGALLRGIEMLISQETGIPCHIADHPLECVAIGAGIALYHLDIIKRNIP
                     TEEEQLVNQYAQY"
     gene            complement(6209..6850)
                     /locus_tag="VNL92_02445"
     CDS             complement(6209..6850)
                     /locus_tag="VNL92_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWO93599.1"
                     /translation="MPIDIPEVVIDRLPVYARALSALAANGHEVISSQDLGSQLGITP
                     AQIRKDLSYFGRFGKQGRGYNVRKLLEELHHILGLDREWTMVLVGVGQLGRAILAYQG
                     FAPQGFRLVAAFDASPERIGEQLGDLVVKDVRLMAESLRDVSVDIGIVAVPAEHAQEV
                     IDALVDNGITAILNYAPIAARVPPHAHLRQIDPVLALQSMTYYLKREGKKAAR"
     gene            complement(6910..7641)
                     /locus_tag="VNL92_02450"
     CDS             complement(6910..7641)
                     /locus_tag="VNL92_02450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWO93600.1"
                     /translation="MAEAPNATRSLDHLGWTRDNQSMFARVSPRYDITNALISLGQDD
                     AWRREAARLAAPRPCRLAIDLATGTGDLGLCLLERCQGVVGVDLTPQMLLEAQRKVEA
                     RDVSERFRLVVADALRLPFADDVFDCATNAFALRNFANPLAALAEMRRVVRPGGRVVS
                     LELTQARPHILEAVHRLYRESVVPLIGAATSPGNLRAYWYLPTSVHRMPTADDLAERM
                     RGLGFRRVWYKLLNFRSIAIHVAEV"
BASE COUNT         1448 a         2514 c         2622 g         1292 t
ORIGIN      
        1 gcttcctcga cgacgtgccg gtcgataagg tgagcgcctt cgaggcagcg ctactcggat
       61 tcatggagag caaccatccg gagattggcc gggccatcga ggaacagaag actctgacag
      121 acgaactgcg ggaacagctg attgccgcta tccgcgaatt caagcaaacc atgccgtact
      181 aggagcgacg agacataacg ttgaggcttg cacgtggcta atatccgcat catccggcga
      241 cgcatccgct ccgtccagag cacggccaag atcacgaagg ctatggagct cgtcgctgca
      301 tcgaagatgc ggcgcgccca ggagcgggct ctcgccggcc gtccctatgc cgagcaactc
      361 cgggaggtgt tgagccatct ggcggagcag acgggtgggg atatcgatcc aaccggggct
      421 accgtccttc atccgctctt ggtccagcgc tcgcccgtcc gccgcgcgct catcatcgag
      481 atcaccaccg accgtggact ctgcggaggc ctgaacgcaa acctgaaccg gcgtacagcg
      541 gcgctcgcgc tcgagttgcg cggtcaggga atcgaggtgt cgctcgtcaa cgtcgggcgg
      601 aaggggcggg agttcttcgg ccgcacggga tacccgatcg aggcggagtt cgcaaacctg
      661 cccgactacc cggccatggc cgacacgctc gccatatcgc atgtcgccgt gaccgacttt
      721 ctcgagggcc gggccgaccg cgtgttgatc gtctacccgc ggttcatcaa cacgacgata
      781 caacggccgg aggtgttgca actgctgccc gtgcaacctc ccgagacgac ggaaaccatc
      841 acggccaagt acgccgatta catcttcgag ccctcacccg aagaggtgct ggagaccctg
      901 gtgccgcgct atgtcgagac gcagctctac gtcacggtgc tcgagaaggc ggccagcgag
      961 caatcggcca gaatggtcgc gatgcgcaat gccagcgatg ccgcggcgga catgatccag
     1021 gacctgaccc tgacctacaa caagacgcgg caggagcaga ttacgaagga gctgctcgac
     1081 gtagtgggtg gggcgcaggc cttgacccgc tgatgcgcga gcgcttcgag gcgctcgttt
     1141 tcgacatgga tggcgtcctt gtggatagcg agccgcttca cctcgaggcg acgcgctcca
     1201 tccttggtgc ggaaggtatc gagattagct gggaagtgtt caaggagtac gttggcacga
     1261 cggtcgaagc gacatggcgc gacatgatgc tgcgatacgg cctcggcggc agctacgagg
     1321 actacctgca gcgctacgag gaggcgattc tcggcgtgtt ggcgagagaa ctcacgcccg
     1381 ccaggggcgc gccggagctg atcgaggcag cgcgggagcg gggtaagcgc ctcgggctcg
     1441 cctcctccgc tagggggacg tggatcgcgg cgaccctcgc cggccttggc ttgacggatg
     1501 tgttcgacgt catcgcatcg ggagatatgg tgacccacgg gaagccggcg cccgacatct
     1561 atctcctcgc ggcacagcgg ctcggcatcg cgcccaagcg ctgcatcgcc atcgaggatg
     1621 cgccaaaggg cgtcgcatcg gcgaagacgg ccggaatgta caccgtcggc gtgctcacgg
     1681 attacaatga cgcagccagc atggcggcgg cagacgagct ggtgaattcg ctggaggagt
     1741 tcaattacga tctcctcgcg gcattcgaag gagcggcctg aggccgcttc ggcaggagga
     1801 agtatggcca cgggaaaggt gacgcaggtc atcggtacag ttgtcgatgc ggagttccca
     1861 ccggacagct tgcccgacct ctacaacgcc gtcgagatcc agatggacgg tggcacgctt
     1921 gttgccgagg tccagcagca cctcggcaac aactgggttc ggtgcctggc tatggacacc
     1981 accgacgggc tacgccgtgg cgtggacgtc ttcgatacgg gccgccccat ctccgtgccc
     2041 gtcggcccat cgacgcttgg tcggttgttc aacgtgctcg gcgagccgct cgataacctc
     2101 ggcgccgtac agacagaagc ttcctggccc atccatcgca tgccgccgag cttcgaacag
     2161 caggagacgc aggcccagac gttggagacg ggcatcaagg tggtcgatct catcgctcca
     2221 ctggcccgtg gaggcaaggt cgggctattc ggcggagcgg gcgtcggcaa gacggtgctc
     2281 atccaggagc tcatccgcaa catcgccgcc gagcatggcg gatactccgt gtttgccggc
     2341 gtcggcgagc gatcgcgcga gggcaacgac ctctggcacg agatgcagga atcgggcgtt
     2401 atcgataaga cggtgctcgt cttcgggcag atgaacgagc cgcccggggt ccgccttcgc
     2461 atcgcgctca ccggcctcac gatggcggag tacttccgcg acgaggaagg ccgcgatgtg
     2521 ctgctcttca tcgacaacat ctaccgctac accctcgccg gcatggaggt atcggcgttg
     2581 ctgggccgca tgccatcggc cgtcggctac cagccaaccc tcgcgaccga gatgggcgat
     2641 ctcgaagagc ggatcacgag cacgaacaag ggctcgatca cgtccttcca agccatctac
     2701 gttccggccg acgactacac cgatccaggt gtctcgacga ccttcgctca cctcgattcg
     2761 acggtcgcgc tcgaccgtgc gctgaccgag cagggcctct acccggcgat ggacccgctg
     2821 agctcgtcgt cgcgcatcct cgacccgcgg gtggtggggc aggagcatta cgacgtggcg
     2881 cgcggcgtgc agcaggtcct gcagcgctac aaggacctgc aggacatcat cgccattctc
     2941 ggtgtcgacg agctttccga agaggacaag ctcgctgtag cccgcgcccg ccgcattcag
     3001 cggtacctca cccagccctt cttcgtcgcc gagggcttca cagggcagcc tggacgcttc
     3061 gtgcccatcc gcgagacggt gcgaggcttc cgcgagatcc tcaacgggga gcacgacgac
     3121 ctgccggagc aggcgttcta catggtcggg accatcgacg aagcggtcga gaagggccgc
     3181 cagatggcca ctgccgcggg gtaggcgcat gccactcacc gtcgaggtca tcacagccga
     3241 gcggcgtgtc ctctccgaag cgggggtcga cgaggtgatc gtgccaggcg cgatgggcga
     3301 actcgccatt ctcccccgcc acgccgcgct catcacggcg ctcgagcctg gggagatgcg
     3361 cttggtccgc ggcggggacg aaacatcatt cgtgctgacg ggaggcttcc tggaggtcaa
     3421 ggacgataag gtcaccgtcc tggcggatgc cgcggagcgc gccgaggaga tcgacgtggc
     3481 gcgggccgag gaagcgcgcc gacgcgccca agagcgcctg gcagaacacg tgagcgccga
     3541 ggagatggct cgggccgaag ctgcgttgcg ccgctcgctc atgcggctcc gtgtcgccga
     3601 acgccgccgc cggcgcggcg gctcctcggg catggggccg ggctccacga ggccaggctc
     3661 gtagggaagc ctgtgcggac acgggcccga ggcggggcac cggcgcatac gaaatacgct
     3721 tgtccctcct gccggcgtgc gttgtcaacc gggacctgcg gcggataagc taggtctatg
     3781 cggggcgccg tcacgactcg atccacctcc agcttcgtca tcgaaggtgg tacgccgctt
     3841 cgaggcgacg tgcacgtgag cggcgcgaag aacgccgttc ttccggcgct cgccgcgacg
     3901 ctcctcaccg acgaagagtg catcatcgag aacgtgcccg acatcggcga cgtgggcgtg
     3961 atgacggcga tcatccagag cgtcggtgcc gaggtcgatg cgaacgatcc ggcctgcctt
     4021 cgagtcgtcg tgccccgcgt gacgcagctc ggcccgccga gcgagctcgt cgtcaacatc
     4081 cgggcgagct tcctcacgat gggtgcgctg ctcggccgtc ttggcgaagc ctcgtgttgc
     4141 ttccccggcg gcgatgttgt gggtcagcgc ccgatcgata cgcatctcgc cggctttcag
     4201 atgctcggcg ccaccgtcgg cagggatggc gagcggttca cggcccgtac aaccggatta
     4261 cgtggcgccc gcatcttcat ggactatccg agtgttctgg gtacagagaa cgtaatgctc
     4321 gccgcgacgc tggcgcgagg acatacgacc atcatcaacg cggcggccga gccagaggtc
     4381 gtgttcctcg cgcggatgct gaactcgatg ggggcgagca tccatggtgc cgggacacac
     4441 acgatcgaca tcgaaggagt ggagcggctg cacgggtgtc acctctcggt catccccgac
     4501 cgcatcgagg ccggcacgtt tctcgtggcg gcggccgcga ctgggggcga tgtcgtcgtt
     4561 cgaggtgcga aaccacgtga tatggacgcc acgctcgcga agcttgccga ggcgggcgtc
     4621 acgatcgagg agctacccga tggcgtccgt gcgcggcgcg agcgcgagct gcacgccatc
     4681 cacctccaag ccctgccgta tccggggctc ccgaccgaca tgcaagctcc catggccgca
     4741 ttgctgacgc aagccgaagg cacgagccag atccacgagc gagtgttcga taatcgcctc
     4801 ctctacgtcg acgagctccg gaagatgggc gcggagatca tcacgagcgg cggtacgacg
     4861 gccatcgtcc tccaacgcac accgctcgtc ggcgcaacgg tccgagcgct cgacgtacgc
     4921 gccggagccg cgctcgtcct cgccggtctc gtcgcggaag gctcaacgac gattctcgac
     4981 gtgtatcatc tcgatcgagg ctatgcggcg ctcgacgaga agctgcgagg ccttggagcg
     5041 cgaatcgagc ggcgcgaggg cgccgttgtg gaacccgccc tgagctaaga accaggccca
     5101 gcgcggagcc caccatctca ccttcgggga atggggctaa caaccaatgc gatttggcaa
     5161 acgaattggg atcgatctgg ggaccgcgaa tgtgctcgtg tacgagcaag ggagaggcat
     5221 cgtcgtcaac gagccggcgg tggtcgcgct cgcacatcgc gacaacacgg tcgtggcggt
     5281 aggcaacgaa gcccgcgcga tgatgggccg cacgccggac agcatctcca tcatccggcc
     5341 gatgcgcgac ggcgttatcg ccgattatct ggtgacggag gcgatgctcc gctacttcat
     5401 cggcaaggcc atcccgcgct tcagcattct gaagccggag gtcatggtgt gcatcccggc
     5461 cggcgtcacg agcgtggagc agcgggccgt gcgagatgcg gcggagcagg cgggtgcgcg
     5521 gagccccgcc catttggtgc ccgagcccat cgccggagcg atcggcgcca acctccccat
     5581 cgacacggcg agcggcaaca tgatcgtcga tatcggtggc gggcgtaccg aggcggccgt
     5641 tatctcgatg tacggcatgg tcgcggccga atcggtgcgc atggccggcg accgcctcga
     5701 cgacgccatc gtcgcctacg tgaagcggcg ccacaatctc gtcattgggg agcggaccgc
     5761 ggaggattgc aagcgacaga ttggatgtgc gattcccgtt gatcccgagt tgcgcatgga
     5821 gatgcgcggc cgcgacgtca tcaccggaat gccgcgtgcc acgacgatca cttccaccga
     5881 gatcgcgaac gcgctccagg ataacctcgc tgtcatgatg catgcgatcc gttccgtctt
     5941 ggaacgcacg ccgccggaac tggcaagcga tgtgatcgac cggggcattg ttctgaccgg
     6001 cgggggagcg ctgctccggg gaatcgagat gctgataagc caggagactg gcatcccctg
     6061 tcacattgca gaccacccgc tggagtgcgt ggctatcggg gccgggatcg cgctctacca
     6121 tctcgatatc atcaagcgga acatcccaac ggaagaagag caactggtca accagtacgc
     6181 gcagtactga ttcgtgctcg gacgaggttc agcgggcggc cttcttgccc tcccgcttca
     6241 gatagtacgt catgctttgc agggcgagca ccgggtcgat ctgccgcaga tgcgcgtgcg
     6301 gtgggacgcg cgcggcgatg ggggcgtagt taaggatggc ggtgatgcca ttatccacga
     6361 gcgcatcgat gacctcttgg gcgtgctcgg cagggacggc gacgatgccg atatcgaccg
     6421 aaacgtcgcg taagctctcc gccatcaacc gcacgtcctt gacgacgagg tctcccaact
     6481 gttcgccgat tcgttccgga gaggcatcga aggcagcgac gaggcggaag ccttgggggg
     6541 cgaagccctg gtaggcaagg atcgcccgac caagctggcc aacgcctacg agaaccatcg
     6601 tccactcgcg atcgagcccg agaatgtggt gcaattcctc cagaagcttc cggacgttgt
     6661 agccgcgtcc ttgcttcccg aagcgcccaa aatagctcag gtcctttcgg atctgcgccg
     6721 gcgtgattcc cagttgcgac ccaaggtcct gggagctgat gacttcgtgc ccgttcgcgg
     6781 cgagggcgga cagagcccgc gcatacaccg gcagtcgatc gatcacgacc tctgggatat
     6841 cgatgggcac ggtcagccgc ctcccttcac catgctcccc cacgggttcc gcgcaccgta
     6901 cgccgcgtgt tacacctccg cgacgtggat ggcgatggaa cgaaagttca gcagcttgta
     6961 ccacaccctt cggaagccga ggccacgcat gcgctcggcc aaatcgtccg cggtcggcat
     7021 gcgatgcacc gatgtcggca ggtaccagta cgcccgcagg ttgcccggcg aggtagctgc
     7081 gccaatgagc ggcacgacgc tttcgcgata gagccggtgg accgcctcca ggatgtgggg
     7141 ccgcgcctgg gtgagctcga gggagacgac gcgcccgccc ggtcgcacga cccgccgcat
     7201 ctcggcgagc gccgcgagtg gattagcaaa gttgcgcagg gcgaaggcgt tcgtcgcgca
     7261 gtcgaacacg tcgtcggcga atgggaggcg gagcgcgtcg gcgacgacga gccggaagcg
     7321 ctccgataca tcgcgcgcct cgaccttccg ttgcgcctcc aacagcattt gcggggtcaa
     7381 gtccacgccc acgacccctt ggcagcgttc gagcaggcaa aggcccaggt cgccggtgcc
     7441 cgtggccaaa tcaatggcga gccggcaagg ccgtggcgcc gccagacggg ccgcttcccg
     7501 ccgccacgca tcgtcctgac cgaggctgat gagagcattc gtgatatcgt agcgaggcga
     7561 gactcgggca aacatgcttt ggttgtcgcg agtccagccg aggtggtcca gcgacctggt
     7621 tgcgttcggt gcttcagcca agttgccctt cgtcgccgcg gaatgcggga atcctagcac
     7681 cggcacaaag tttgcgtcaa tctttgtacg cacaatcacg ctagactcgt tggggacacc
     7741 gtcacggagg cccaagcatc gaatgagcga cgatctgctg gcccgggtaa cgcggcgtgg
     7801 cgagaacaac ccattctggc agacgatcgg actcgcggtg gtcgatgccg gcgagggatg
     7861 ggcccgcctt cgtctg
//
LOCUS       DATMBB010000113         5356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_97958, whole genome shotgun sequence.
ACCESSION   DATMBB010000113 DATMBB010000000
VERSION     DATMBB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5356
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97958"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1572)
                     /locus_tag="VNL92_02455"
     CDS             complement(70..1572)
                     /locus_tag="VNL92_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWO93601.1"
                     /translation="MTAREQQASGFDPRWLTLIVTTIGTFMSILDATIVNIALPDILR
                     DFHSDLDQGQLVLSVYLLALAIVIPVSGYMAERFGMKRMYMITLVLFTAGSALCGIAW
                     NVESLIFFRVLQGLGGGMLQPLGMAIVFSMITPLERGRFMGLLGLPMLLAPIIGPTVG
                     GYLVEYSTWRMIFMINLPVGIINLVLAYMLLKETPLREGVRLDVRGFALAAIAFPALI
                     LALSEGEHWGWTSPGIASMFAVGVVAIAAFIRTELRHDDPLLRVALFREPIFALGMGI
                     QFVMQFSLFGLQFLLPLFLQTAHRLSPAETGLVLLPSGIATFITMNFSGRTYNRFGPR
                     VLAVIGLLILIATTGLFSRTTEHTSILTIGALATLRGAAMGFCMMPVQTALFNTVPQQ
                     FVTRATALTNVGFRLYGAVSTAVLTTVLTLSLGWHGAPEGSSITRGTAPLNFMSKAFD
                     DAFLLMTGVTAIALLLALFLRDPVLEAVRRGEEEPRPEPVPEGSAASAEI"
     gene            complement(1640..2962)
                     /locus_tag="VNL92_02460"
     CDS             complement(1640..2962)
                     /locus_tag="VNL92_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWO93602.1"
                     /translation="MSTPLELLTAQRPNMDACIRCGLCLSVCPTYQTTIMEEESPRGR
                     IAMARAVAAGVVPLDAEIVRHQESCLLCEACTAICPAGFRMEEMGIAVRRAVRESGKV
                     GRMRRMALRFAFRGPLAHMWLLRLLSRGLWLYQRSGSQWLARRSGLLRLLRLARVERL
                     TPRMPARFFVAKGQSWPPSTPAADDAPRVGLFAGCVMSTAFAETDEATARVLAANGCE
                     VVAPAGQGCCGALQAHSGAWHEARERARRVIDSFQQPFDAIVVNAAGCGSAMKGYGHL
                     LADDPDYAEKAARFSERVRDVAEFLVSRPLNGNLGPLDATVTYQEPCHLAYAQRIRNA
                     PRELLTAIPGLTLAEMKESALCCGSAGIYNVTQGAMAESLLDRKLENALATNASIIVT
                     GNPGCHIQLAAGIHDRGSNVRIAHVVDLLDEAYRRAEDSSSHAEPTID"
     gene            complement(3015..4079)
                     /locus_tag="VNL92_02465"
     CDS             complement(3015..4079)
                     /locus_tag="VNL92_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWO93603.1"
                     /translation="MIQDGIVTVHHEALRRQVAALFHALGVPEGDADVAADVLVQADL
                     RGVESHGVSNYINLIYVPGLKAGNINPHPRIHVEHARHATAVINGDGGLGMVVGHFAM
                     SEAIHLARDAGAAWVTARNSRHFGMAGYYAMMALEHDMIGFSFTNAGPLQVPTYGRQP
                     MLGTNPIAFAAPAGEEWPFVLDMATSTVAIGKVIGAMRTGGLIPLGWAVDSEGRPTSD
                     AGAAVQARALMPLGGAAETGGYKGYGLATMVDILSGVLSGAGFSAALAGGEVGHCFGA
                     ICIDAFRPAAEFKSTMDEAIRALRNSAKAEGAERIYVAGEKEFETERERRTNGIPLHP
                     EVVETLRRLSDEAKAPWTLT"
     gene            complement(4083..>5356)
                     /locus_tag="VNL92_02470"
     CDS             complement(4083..>5356)
                     /locus_tag="VNL92_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO93604.1"
                     /translation="LPMVLRRAFKVAAEPPQGPVFLSMPIDVLDQEADMHIVPTTYVD
                     CRSRPSEDTVRRAAEMLLGAQNPLIVVGDGVAVSGAQDEVAQLAEATGARIMSAFASE
                     MNVRTDHPLYAGMLLVISGEAMRAQLEGVDLLFAIGTPVFTQLFPTAGMLLPEGMRLM
                     QLDLDSWEIAKNFPVDLGMQADPREGLRAIITAVQARRSSEVGAVAQERAAQLAAVRE
                     QMDLMAREALERQWNQTPISVARLMKQLADVVPEGTIVFDESITSSGALQRYFTFTEP
                     GSLFRARGGGLGPGMPGTIGVKLARPDRPVVGVVGDGAALYTIQSLWSAAHHKVNVTW
                     VICNNQSYRILKINMLQYLGEAGRGRQFVAMDLTDPGINFAHVAESFGVRGWRVEDPD
                     ELEPALRSAIALDGPALVDVVIDGSLGPLLG"
BASE COUNT          941 a         1724 c         1816 g          875 t
ORIGIN      
        1 ggtgaagtcc tggaggtgcg gccataggct gcgagccgtt gcgcggccga tggcgaaagg
       61 cgctgggggc tagatttccg cggacgccgc cgatccctcg ggtacgggct ccggccgcgg
      121 ctcctcctcg cctcgccgca cagcttcgag gaccgggtcg cgcaggaaca acgcgagaag
      181 aagggcgatg gccgtgacgc ccgtcatgag gaggaaggca tcgtcgaacg ccttggacat
      241 gaagttgagc ggagcggtac cacgcgtgat cgacgagccc tcgggtgctc catgccatcc
      301 gaggctcagc gtcagcactg tggtcaggac ggcggtgctc acggcgccgt agagccggaa
      361 ccctacgtta gtgagggccg tcgccctcgt cacgaactgc tgtgggacgg tgttgaacag
      421 ggccgtctgc accggcatca tgcagaatcc catcgcggcg cccctgagcg tggcgagcgc
      481 accgatcgtg aggatgctcg tgtgttcggt cgtgcggcta aacagccctg tcgtcgcgat
      541 gaggatgaga aggccaatca cggcgagcac ccgtggcccg aagcggttgt aggtgcgccc
      601 cgagaagttc atcgtgatga acgttgcgat gccgctcgga aggagcacaa gtcccgtctc
      661 ggccggggac aacctgtggg cggtctgcaa gaacaggggt agcaggaact gcaggccgaa
      721 gagggagaac tgcatgacga actgaatgcc catgcccagc gcgaagatgg gctcgcgaaa
      781 caacgccacg cggagcaacg gatcatcgtg acgcagctcc gtccggatga acgccgcgat
      841 cgcgacgacg cccaccgcga acatcgacgc gattccaggc gaggtccatc cccagtgctc
      901 gccctcgctc agcgcgagaa tgagcgccgg gaaggcgatt gctgcaaggg cgaagcctcg
      961 cacgtcgagc cgcacgccct cccgcagtgg tgtttccttc agcagcatgt acgccaggac
     1021 gaggttgatg atgccgacgg gcagattgat catgaagatc attcgccacg tggagtactc
     1081 gacgaggtat ccgccgaccg tcggcccgat gatgggcgcc aacagcatcg gtagtccgag
     1141 gagtcccatg aatcggccgc gttccagagg cgtgatcatg ctgaagacga tggccatccc
     1201 gagaggctgg agcatgccgc ctcccaggcc ctggagcacg cggaagaaga tgaggctctc
     1261 gacgttccag gcgattccgc acaaggcgga accagccgtg aagaggacga gcgtgatcat
     1321 gtacatgcgc ttcatgccga agcgctccgc catgtagccg ctcaccggga tgacgatggc
     1381 cagcgccagc aaatagacgc tgaggacgag ctggccttgg tcgagatcgg aatggaagtc
     1441 gcgcaggatg tcgggcaggg cgatgttgac gatcgtcgcg tcgaggatcg acatgaacgt
     1501 gccgatggtg gtgacaatca gtgtcagcca ccttgggtcg aatccggacg cttgttgttc
     1561 ccgcgctgtc atgccctcga cataacctcc tcgctaacct tcacgtgcac gtaaaggata
     1621 cgggaggtga gggtagcggt caatcgatgg tgggttccgc gtggctggag gagtcctccg
     1681 cccggcgata cgcctcgtcg aggaggtcga ccacgtgcgc gatccgcacg ttggagccgc
     1741 ggtcatggat gccggctgcg agctggatgt ggcagcccgg atttccagtg acgatgatgc
     1801 tcgcgttcgt cgcaagggca ttctcgagct tgcggtcgag caggctctcg gccatcgcgc
     1861 cctgggtaac gttgtagatt cccgcactgc cgcagcagag cgcgctctcc ttcatctccg
     1921 ccagcgtgag ccccggaatc gccgttagca gctccctcgg cgcatttcgg atgcgttggg
     1981 catatgcgag gtggcacggc tcctggtagg tgactgtcgc atcgagggga ccgaggttcc
     2041 cgttgagcgg ccgcgacacg aggaactccg cgacgtcgcg gactcgctcg gagaagcgcg
     2101 ccgccttctc ggcgtagtcc ggatcgtcgg cgaggagatg gccgtagccc ttcatggccg
     2161 acccgcaccc cgcggcattg acgacgatcg cgtcgaacgg ctgctggaac gagtcgatga
     2221 cgcggcgagc acgttctcgc gcctcgtgcc aggcgccgct gtgtgcctgg agcgcgccgc
     2281 agcagccttg tcccgccggc gccacgacct cgcagccgtt ggcggcgagg actcgggccg
     2341 tggcttcgtc cgtctcggcg aaggcggtgc tcatcacgca gccggcgaag agcccgaccc
     2401 ggggcgcgtc atctgccgct ggtgtggagg ggggccagct ctggcccttg gccacgaaga
     2461 agcgcgccgg catgcgggga gtcagccgtt cgacacgcgc cagacggagc aggcgaagca
     2521 acccggagcg tcgcgcgagc cattgcgacc cggatcgctg atacagccat agaccgcgcg
     2581 agagaaggcg cagaagccac atgtgcgcga ggggcccgcg gaaggcgaac cgcagagcca
     2641 tccgcctcat gcggccgacc ttgcccgact cgcggactgc tcgccggact gcgatgccca
     2701 tctcctccat gcggaagccc gccggacaaa ttgccgtgca cgcctcgcac aggaggcagc
     2761 tttcctggtg ccggacgatc tcggcgtcca gcggaaccac gcccgccgcg acggcccgag
     2821 ccatcgcgat cctgccgcgc ggcgactcct cctccattat ggtggtctga tacgtagggc
     2881 agaccgacag acagagcccg caacggatgc aggcatccat gttcggccgc tgggccgtga
     2941 gaagctcgag aggagtactc atgtagttac gggatcaagt atggccccgg ggaggctggt
     3001 gcgtcgagcg cgccctaggt caaggtccag ggggctttgg cctcgtcgga gagccggcgc
     3061 agcgtctcga cgacctcggg atgcaggggg atgccgttgg tgcgccgttc gcgctccgtc
     3121 tcgaattcct tttccccggc gacgtagatg cgctccgccc cctcggcctt tgccgagttg
     3181 cgcagagccc ggatcgcctc atccatcgtc gacttgaact cggcggcggg ccggaatgca
     3241 tcgatgcaga tggcgccgaa gcaatggccc acctcgccgc cggccagcgc cgcactgaac
     3301 cccgccccgc taagcacgcc ggagaggatg tcgaccattg ttgctaggcc atagcccttg
     3361 tatccgccgg tttcggccgc gccaccaagc ggcatgaggg cgcgtgcctg gacggccgca
     3421 cccgcatcgc tcgtcgggcg gccctccgaa tcaacggccc agcccagcgg gatgaggccg
     3481 cccgttcgca tggcgccgat gaccttgccg atggcgacag tgctcgtggc catgtcgagg
     3541 acgaatggcc actcctcgcc ggccggcgcc gcgaacgcaa tggggttcgt gcccagcatc
     3601 ggctggcggc cgtacgtcgg tacctgcagg gggccggcat tcgtgaagct gaacccgatc
     3661 atgtcgtgct cgagggccat catcgcgtag tatcctgcca tcccgaagtg gcgcgagttg
     3721 cgggctgtca cccacgccgc gccggcgtct cgagccaggt ggatcgcctc cgacatcgcg
     3781 aaatggccca ccaccatccc gagtccgccg tctccattga tcacggccgt cgcgtgcctc
     3841 gcatgctcga cgtggattcg aggatgagga ttgatgttgc ccgccttcag gcccggcaca
     3901 tagatcaggt tgatgtagtt ggagacgcca tgcgattcca ctccgcgcag gtccgcttga
     3961 accagcacgt cggctgccac gtccgcgtcg ccctccggca cgccgagcgc gtggaacaac
     4021 gctgcgactt ggcgtcgtag cgcctcgtga tgaacggtga cgatgccgtc ctggatcatc
     4081 ggctaaccta ggagcggtcc gagcgagccg tcgatcacga catccacgag ggcggggcca
     4141 tcgagcgcga tggcggatcg cagggcaggc tcaagctcgt ccgggtcctc gacgcgccag
     4201 ccccgtacgc cgaatgactc ggcgacgtgc gcgaagttga tcccgggatc ggtgaggtcc
     4261 atggcgacga actggcgtcc tcgtccggcc tcgccgaggt actggagcat gttgatcttg
     4321 aggatccggt agctctggtt gttgcagatg acccaggtca cgttgacctt gtggtgggcc
     4381 gccgaccaga gcgactggat ggtgtagagc gctgcgccat cgccgacgac gccaacgaca
     4441 gggcgatcgg gtcgcgcgag cttcacacct atcgtgcccg gcatgccggg tccgaggcca
     4501 ccgccgcggg cgcggaagag cgaacccggc tcggtgaagg tgaagtatcg ctgcaatgcc
     4561 ccggatgacg tgatcgactc gtcgaagacg atcgtgcctt cgggcacgac atcggccagt
     4621 tgcttcatga gcctggccac cgaaatcggc gtttggttcc actggcgttc caatgcttcg
     4681 cgcgccatta ggtccatctg ctcgcggacg gcggcgagct gcgccgccct ttcttgcgcc
     4741 accgcgccga cctcggacga gcgccgagcc tgcactgcgg tgatgatggc gcgaagcccc
     4801 tcgcgcgggt cggcctgcat cccgaggtcg accgggaagt tcttggcgat ctcccacgag
     4861 tccaggtcga gctgcatgag gcgcataccc tccggcagga gcatgcccgc cgttgggaac
     4921 agttgcgtga agaccggcgt accgatggcg aagagaaggt cgacgccctc cagctgggcc
     4981 cgcatcgcct ccccggagat gaccagcagc atgcccgcat agagagggtg atcggtccgc
     5041 acgttcatct cggaggcgaa cgcgctcatg atgcgggctc ccgtagcttc ggccagctgg
     5101 gccacctcgt cctgcgcccc ggaaactgcg acgccatcgc cgacgacgat cagcggattc
     5161 tgcgcgccga gcagcatctc ggccgctcgc ctgaccgtat cttcagaagg acgggagcgg
     5221 caatcgacgt acgtggtcgg aacgatgtgc atgtccgcct cttggtcgag cacatcgatt
     5281 ggcatcgaga gaaacaccgg cccttggggc ggctccgcgg cgactttgaa ggcgcggcgg
     5341 agcaccatcg gcaggg
//
LOCUS       DATMBB010000114         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_253519, whole genome shotgun sequence.
ACCESSION   DATMBB010000114 DATMBB010000000
VERSION     DATMBB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253519"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VNL92_02475"
     CDS             complement(<1..627)
                     /locus_tag="VNL92_02475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWO93605.1"
                     /translation="MDATGTVGVVDNQNTRSVESVGVGDAIAGMPARAAEQSSIAIPL
                     APAQEPHFGYFATKRAVDIAVALLALIALSPLFLVVAVAIKLDSRGPVFYRHTRVGER
                     GRWFQCYKFRSMEEGADARWRELRHLDITGGPTFKCERDPRVTRVGRFIRRTSIDELP
                     QLFNVLRGDMSLVGPRPPLPAEVDAYTDYETARLSVAPGITCLWQVSGR"
     gene            complement(767..952)
                     /locus_tag="VNL92_02480"
     CDS             complement(767..952)
                     /locus_tag="VNL92_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93606.1"
                     /translation="MMLTRRISGGYWWAIGIALVAVPALVALSAAPVGAQYGPLQFFL
                     FFLRFLLRSIFGRFGFF"
     gene            complement(1281..>2945)
                     /locus_tag="VNL92_02485"
     CDS             complement(1281..>2945)
                     /locus_tag="VNL92_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HWO93607.1"
                     /translation="AGWLPGLSQQKDGLEELISLADGLATAGIAGVRTLQVLPSDAQS
                     DGIGRELIAISESHRGDIGLVASEAAEAIRASSELADDDLIGPLGDARDELLGYRRDL
                     DDLYTMASFALLAPTLLGADGSTSYLVLGMNSGELMPGGGFIGNYAIFDVAQGEVQDI
                     QGEDVYQLYDRWTRSPDFVRVDPPPALERYLLGEEWSWALGEAAWYPDFPTTASAAQE
                     FVRLGGVGTTDATVGLTVPAMEELLRLTGPIELPEFGITFRAEDVGLRLSFDITHDEA
                     GARLFAAFMREMSNRLMALERSSWRSAWEVVRGLVDREELFINFADSDVQKRVAELGW
                     DGRLPPPSGEQRVLLTDASVRSTKLNFALQRRAWLVIDLSGDRPRHRLRVTYRNAYSE
                     WAAGKDPALAQQVLGPTNMLGNYIQLFAPAGSELLRLVEVFSGGATERDLPPEPEEEQ
                     LGYSVFATFLPVRPDSERTVEFEYELPAGSSAQVDGHRYRLQLEKQPGVPAYPIDILI
                     VPPDGRAVERIEVCAPTRDACASQTDALVSTELDADLTLVAQFGER"
BASE COUNT          527 a         1003 c          949 g          466 t
ORIGIN      
        1 ccggcccgat acctgccaca gacaggtgat ccccggcgct acgctcagac gcgccgtctc
       61 gtaatcggtg taggcatcca cctccgccgg caagggcggt cgaggcccca cgaggctcat
      121 gtccccacgc agcacgttga acagctgcgg caactcgtcg atgctcgtcc tccggataaa
      181 ccgccccacc cgcgtcaccc gcgggtcgcg ctcgcacttg aaggtcgggc cccctgtgat
      241 gtccaggtga cgcagctctc gccaccgcgc gtccgcaccc tcctccatcg accggaactt
      301 gtagcactgg aaccaccgtc cccgctcgcc taccctcgta tgccggtaga acaccgggcc
      361 gcgcgagtcg agcttgatcg ccacggccac gacgaggaac aaggggctca gtgcgatgag
      421 ggcaaggagc gccaccgcga tgtccaccgc ccgcttcgtc gcgaagtagc cgaaatgggg
      481 ctcctgtgcc ggggcgaggg ggatggcgat ggaggactgc tcagcggcgc gcgccggcat
      541 gccggcgatg gcatcgccga cgcctacgga ctcgactgag cgcgtgtttt ggttgtctac
      601 gacacctact gttcccgtag cgtccatgga gcggaacccc ctggcagatg gtttgcgttc
      661 ccccgagcgc aaaccgatga acggaaacca ccctagcgtg gctgcatatg cgagccgccg
      721 tcggcgatcg tcgaatccct aagctgaagc cgcgaaggcc gccctactag aagaagccaa
      781 atctgccgaa gatggaccgg agcaggaacc ggagaaagaa caagaagaac tgcagcggcc
      841 catactgggc gccgaccggc gccgcagaga gcgccacgag cgcgggaacc gctacgaggg
      901 cgatgccgat cgcccaccag tatccgccgg aaatccgtcg tgtcagcatc attccccctc
      961 cgggcgacct atcagaagcg caggcccagg gtcgggtcct tctcgcttac tgggacgaat
     1021 tattgcgcac agcgttctca tgatcaagta gcttcggtga agatcgtgtt acggtgatat
     1081 ctgactcttc taccgcctcc gaccaatcca tcgcctcctt cacctgagct atatgcggca
     1141 gcatatccag cgatgtcgac acggtcatcg ggggatcacc taggaaacgt taagattgtg
     1201 tatcggggga tcgggcaggt gtatcgcggg aacaccccga ggtccacgcg ttcgccgacg
     1261 gacttgcgcg aacggacgcc ctagcgctcc ccgaactgcg ccaccagcgt cagatccgca
     1321 tcgagctcgg ttgagaccaa cgcatcggtc tgcgatgcgc acgcgtctcg cgtcggtgcg
     1381 caaacctcga tacgctcgac cgcgcggccg tcgggcggca cgatgaggat gtcgatcggg
     1441 tacgccggaa cgccgggctg cttctccagc tgaagccggt agcgatggcc gtccacctgc
     1501 gcgctcgaac cggcgggcag ctcgtattcg aactccaccg tccgctcgct atcgggccgt
     1561 accggcagaa acgtggcaaa gacgctatag ccgagctgtt cctcctcagg ctcgggagga
     1621 aggtcgcgct cggtggcgcc cccggagaag acctccacaa gcctcaggag ctcgctgcca
     1681 gcaggcgcaa agagctggat gtagttgccc agcatgttcg tcggccccaa gacctgctgc
     1741 gcgagagccg ggtccttgcc ggcggcccat tcgctgtacg cgttgcggta cgtcacccgc
     1801 agccggtggc gggggcggtc gcccgacagg tcaatcacga gccatgcccg gcgctggagg
     1861 gcgaagttga gcttcgtgct tcgcacactg gcgtcggtga gcaggacgcg ctgctctccc
     1921 gatggtggcg gaaggcggcc atcccagccc agctccgcga ccctcttctg cacgtcgctg
     1981 tccgcgaagt tgatgaagag ctcttcgcgg tcgacgagac ctcgaaccac ctcccaggcg
     2041 gagcgccagg atgatcgttc gagggccatg aggcggttcg acatctcgcg catgaatgcc
     2101 gcgaagagcc gcgcgcccgc ctcgtcgtgg gtgatgtcga acgagaggcg caggcccacg
     2161 tcctcggccc ggaacgtgat cccgaactcc ggcagctcga tcggcccggt gagccggagc
     2221 agctcctcca tggcggggac ggtcagccca acggtggcgt cggtcgtccc cacaccacca
     2281 agacgcacga actcttgcgc cgcgctcgcc gtcgtcggga agtccggata ccaggccgcc
     2341 tcgccgagtg cccacgacca ctcctcgccc aggaggtacc gctcgagggc tgggggaggg
     2401 tcgacacgaa cgaagtcggg ggaccgcgtc cagcggtcgt agagctggta gacatcttcg
     2461 ccctggatgt cctggacctc gccctgggcc acgtcgaaaa tggcgtagtt gccgatgaat
     2521 ccgccgccgg gcatgagctc cccgctgttc atgccgagca ccaggtagct cgtcgatcca
     2581 tcggcgccga ggagcgtggg cgcgaggaga gcgaacgacg ccatggtata caggtcgtcg
     2641 aggtccctcc gatagccgag gagctcgtcg cgcgcatccc cgagcggccc gatgaggtcg
     2701 tcgtccgcaa gttcgctact ggcgcgaatg gcctccgccg cctcgctcgc gacgaggccg
     2761 atatcgcccc ggtgggactc actgatggcg atgagctctc gcccgatccc gtcgctctgc
     2821 gcgtccgacg gaaggacctg gagcgtgcgg acgccggcta tgccggccgt ggcgaggcca
     2881 tcggcgaggg agatgagctc ctccaggccg tccttctgct ggctgagccc cggcagccaa
     2941 cccgc
//
LOCUS       DATMBB010000115         3625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_484019, whole genome shotgun sequence.
ACCESSION   DATMBB010000115 DATMBB010000000
VERSION     DATMBB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484019"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="VNL92_02490"
     CDS             complement(<1..1495)
                     /locus_tag="VNL92_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HWO93608.1"
                     /translation="MVLRPGRLRRYGEITGVLARHGFGLALWRIGAGRLVPFHRGWLG
                     HARRPEPYSEAEHLRMAFEELGVTFIKLGQILSTRPDILPPEYIEQLAKLRDAVPPVA
                     AAAIVDTIERELGASIQRLFLEFDDTPLASASIGQVHAARLSDGTEVVVKVQKPGVAE
                     RVEADLAVLRQLAEAAERRSQTARRFGAVGLVDEFAWQLRAELDYVREAHNAQRFADQ
                     FRDNRMVRVPCIHERLSTGAVLTMERVRGTRIGDVEALSLQGIDRKALAERSARIVLE
                     SVFRHGFYHADPHPGNFFVQPDGSIAVLDFGMMGHLSDETRRTMVRLLVAGLEEDTDA
                     VVDGFAELGVRPPPDAREGFGRDVQHMLDAYYGLELGEVSAEHFSKDIASIARRHALR
                     LPSELALLLKTIAMNEGVGRELDPSFNLVTVAEPFARDAMSELHSPIALAKRATRSAI
                     DTAGLAVELPERARRILRQVERGDFEVRLRRDEIQAALDGFGAIMNRL"
     gene            complement(1515..2144)
                     /locus_tag="VNL92_02495"
     CDS             complement(1515..2144)
                     /locus_tag="VNL92_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWO93609.1"
                     /translation="MALVHLDLAPKAGRWDNEQRHIGWLEEESMNEKRWLTLVIVALS
                     LSAALLVACGDDDDDDDGEDGADDVGRVEHTTGMTAWQGEEVVIRASDFAFDPPELRV
                     GQQERYMLHLVNDSGQLHDWSIDAMPATEVRVLESAEHEMDEDHASPMPDDMGTAMPG
                     ESMARLHVAAEAGMDRHISFVPMEKGEYTFYCTVGNHREAGMEGKLFVE"
     gene            2278..2541
                     /locus_tag="VNL92_02500"
     CDS             2278..2541
                     /locus_tag="VNL92_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HWO93610.1"
                     /translation="MWHMGDGWGWWMVFAWIWAAVFWGLIIWGIVTIVNRTSEGAAPG
                     GRRDAGQDERSALDILERRYASGELTEEDFERMRDRIVSPRGP"
     gene            2593..3276
                     /locus_tag="VNL92_02505"
     CDS             2593..3276
                     /locus_tag="VNL92_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93611.1"
                     /translation="MTTAPVKSPPETGPIVDVDHEATRVTQRRYARLARLYDAREWIA
                     ERAMLGRLRRAFWTLVPAGGHILEVGVGTGKNMPLYPAAARVTAIDLVPAMAERAMLR
                     ATKVGANVDLALMDAQELALRDATFDIVAATFVFCSVPDPILGLREVRRVLRPEGRLF
                     LMEHVRAENAVLGWIMDRVNPLAVRLSGANINRRTVENVRAAGFDVRSVRSRAGLIRL
                     IEAVPSADA"
     gene            3281..>3625
                     /locus_tag="VNL92_02510"
     CDS             3281..>3625
                     /locus_tag="VNL92_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWO93612.1"
                     /translation="MRERPIAVEPLAERIMRVRVEHVAKTYVTGGMWPFTRRNPVLTD
                     ASLDVPAGEIAAIVGGNGSGKSTLMMIIAGVLDRDAGDVRIDGRIGYCPQAPVLYEKL
                     TVAETFRLFGVAY"
BASE COUNT          588 a         1275 c         1178 g          584 t
ORIGIN      
        1 caagccgatt cataatcgcg ccaaagccat cgagcgccgc ctggatctcg tcgcgtcgca
       61 ggcgcacctc gaaatcgccc cgctcgacct ggcgaaggat gcgtcgcgct cgctccggca
      121 actcgacggc gaggcccgcc gtatcgatcg cggatcgcgt ggctcgcttc gccaaggcga
      181 tgggcgagtg cagctccgac atcgcgtcgc gggcgaaggg ctccgccacg gtgacgaggt
      241 tgaacgacgg gtctagctcg cgcccgacgc cctcgttcat cgcgatcgtc ttcagcagga
      301 gggccagctc cgaggggagt cgcagggcgt ggcgccgcgc gatggaagcg atgtccttgc
      361 tgaagtgctc cgccgatacc tcccccaact cgaggccata gtaggcgtcg agcatgtgct
      421 gcacgtcccg cccaaagccc tcccgcgcgt cgggcggggg gcgaacaccg agctctgcga
      481 acccgtccac cacggcatcg gtgtcctcct ccagccccgc taccaacagc cgcaccatgg
      541 tgcggcgcgt ctcgtccgag aggtgaccca tcatcccgaa gtcgagtacg gcgatacttc
      601 cgtcgggctg gacgaagaag tttccggggt gcggatcggc gtgatagaag ccgtgccgga
      661 ataccgactc gaggacgatc cgggcgctcc gctccgccag cgccttccta tcgatgcctt
      721 gaagggagag ggcctcgacg tcgccgatgc gggtgccgcg gacgcgctcc atggtgagca
      781 cggcgccggt gctcaaccgc tcgtggatgc agggcacccg caccatgcga ttgtcgcgaa
      841 actgatcggc gaaacgctgg gcgttatggg cctcgcgtac gtagtcgagc tccgctcgga
      901 gctgccaagc aaactcgtcg acgaggccga cggcgccgaa gcggcgcgcc gtctgggagc
      961 gccgctccgc cgcttcggcg agctgacgga gcacggcgag atcggcctcc actcgctccg
     1021 ccacgcccgg cttctgcacc ttgacgacga cctcggtccc atcggacagc cgggccgcat
     1081 gcacctgacc aatgctggcg gaagcgagcg gcgtatcgtc gaactccagg aagagccgct
     1141 ggatcgaggc tccgagttcg cgctcgatcg tatccacgat ggccgcggcg gctacgggcg
     1201 gcacggcatc gcgcagcttc gctagctgct cgatgtactc cggcggcagg atgtccggcc
     1261 tcgtactcag gatctggccg agcttgatga aggtgacacc gagctcttcg aaggccatcc
     1321 gcaggtgctc ggcttccgaa tacggctccg gccggcgggc atgcccaagc cagccgcgat
     1381 gaaacggaac caagcggccc gcgccgatgc gccaaagggc caagccaaag ccatgccgcg
     1441 cgagaacacc cgtgatctcg ccgtagcgcc gcagacgacc cgggcggagg accatcgctc
     1501 cccctattcg ctcgctactc gacgaacagc ttgccttcca tgccggcctc gcggtggttc
     1561 ccaacagtgc agtagaacgt gtactcgccc ttctccatcg ggacaaagct gatgtggcgg
     1621 tccatgcctg cctctgcggc cacgtgcaac ctagccatgc tctcgccggg catggcggtt
     1681 cccatatcgt ctggcatcgg cgaggcgtga tcctcgtcca tctcgtgctc ggccgattcg
     1741 aggacccgca cctccgtcgc gggcatcgcg tcgatgctcc agtcgtgcag ttggccgcta
     1801 tcgttcacga ggtgcagcat gtagcgctcc tgctgcccca cccgcagttc cggcggatcg
     1861 aaggcgaagt cgctcgcgcg gatgaccacc tcctcgcctt gccacgccgt catgccggtc
     1921 gtgtgctcca cacgtcccac atcatcggcg ccatcctcgc catcatcatc gtcatcgtcg
     1981 tcgccgcagg cgaccagcag cgccgcgctc aagctcagcg cgacaatgac caacgtcagc
     2041 caccgtttct cgttcatgct ctcctcctcg agccagccaa tgtgcctttg ttcattgtcc
     2101 cagcggccgg ccttcggcgc gaggtccaaa tggaccaggg ccatcgggac gagaccctcc
     2161 cgggacggga cgaacggcac tgccacccct ccgcccctct gggtactatc ggagcgtgac
     2221 gctgccagtg gctcgccgcc ccggccctgg gaatcggcga ctggaaggag agctgtcatg
     2281 tggcacatgg gcgacggctg gggatggtgg atggtgttcg catggatctg ggccgccgtg
     2341 ttctggggcc tcatcatctg gggcatcgtc accatcgtga accggaccag cgagggtgcg
     2401 gcgccgggcg gacgtcgcga tgcgggccag gacgaacgca gcgccctcga catcctcgaa
     2461 cggcgttacg ccagcggcga gctgaccgaa gaagacttcg aacgcatgcg cgaccgcatc
     2521 gtgagcccgc gaggtccata accccggccg ggatctgatc catccgcgca ctccgagagg
     2581 aggaagcccg cgatgacgac cgcaccggtg aaatcgcccc cggagacggg gcccatcgtc
     2641 gatgtcgatc atgaggcgac acgcgttacg cagcgacggt acgcgcggct cgcccgcctc
     2701 tacgacgccc gggagtggat cgccgagcgc gccatgctgg gccgactgcg ccgcgcattc
     2761 tggaccttgg tccccgccgg cggccacatc ctcgaggtcg gcgtgggcac gggcaagaac
     2821 atgccgctgt acccggccgc ggcgcgggtc acggcgatcg acctcgtccc agcgatggcg
     2881 gaacgagcga tgctccgggc gacgaaggtt ggggccaacg ttgacctcgc cctgatggac
     2941 gcgcaggagc tcgccctccg cgacgcgacc ttcgatatcg tcgccgcgac gttcgtgttc
     3001 tgctccgtcc ccgacccgat cctcgggctt cgcgaggtcc ggcgggtcct ccgcccggag
     3061 ggccgcctct tcctcatgga gcacgtgcgg gccgaaaacg ccgtgctcgg ctggatcatg
     3121 gaccgcgtca atcccctggc cgtgcggctc tccggcgcca acatcaaccg acggactgtc
     3181 gagaacgtgc gggcggcggg attcgacgtg cggtccgtcc ggtcgcgcgc cggtctcatc
     3241 cggctcatcg aagccgttcc ctccgccgat gcctgacatc gtgagggaac gccccatcgc
     3301 ggtcgagcct ctcgcggagc gcatcatgcg cgtccgggtc gagcacgtcg ccaagacata
     3361 cgtgaccggg ggcatgtggc ccttcacccg gcgcaacccg gtgctcaccg acgcctcgct
     3421 tgatgtgcct gccggtgaga tcgccgccat cgtcgggggg aacggctccg gcaagtcgac
     3481 gctcatgatg atcatcgccg gcgtgctgga ccgcgacgcg ggcgatgtgc gcatcgatgg
     3541 acgtatcggc tactgtccac aggcgcccgt tctctacgag aagctgacag tggccgagac
     3601 cttccggctg ttcggcgtgg cctac
//
LOCUS       DATMBB010000116         2373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_49825, whole genome shotgun sequence.
ACCESSION   DATMBB010000116 DATMBB010000000
VERSION     DATMBB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49825"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VNL92_02515"
     CDS             <1..371
                     /locus_tag="VNL92_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWO93613.1"
                     /translation="GRPLQSSEIAARQAIPEQYLDQLLTTLRRAGLIRSVRGPQGGHA
                     LLRRPEEIRASEVVIAMEGSLDPIACLEPGSPCTLQGGCAQRSMWEDVRAAILGVLDA
                     TTIADLRDRALAPTATRYVI"
     gene            388..1326
                     /gene="cysK"
                     /locus_tag="VNL92_02520"
     CDS             388..1326
                     /gene="cysK"
                     /locus_tag="VNL92_02520"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWO93614.1"
                     /translation="MSDRIALEGRALIADSALDLIGNTPIVRLHRVVPSGSAEVLGKL
                     ESMNPAGSVKDRIALAMVSEAERAGRLHPGDTIVEPTSGNTGIGLAMVAAVKGYRLIL
                     TMPEDMSVERRKLLARYGVEFVLTPAIEGMTGAVFTAQELAEKHGYFMPQQFENPANP
                     EVHRRTTAREVLEATDGQLDAFVAGVGTGGTITGVGQVLKERVPHVLVIGVEPARSPV
                     LQGGRAGLHAIQGIGASFVPGVLDRSVMDEVMSVSDEAALEMMRRLTREEGLLVGVSA
                     GANVEAAIQVAKRLGPGKRVVTVLPDTGERYLSMPA"
     gene            1414..>2373
                     /locus_tag="VNL92_02525"
     CDS             1414..>2373
                     /locus_tag="VNL92_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWO93615.1"
                     /translation="MSINVYIPAPFRRLTGNRSSVHSSAADVNSLLDDLDRQFPGFRS
                     LLCNERGEVPTHINIYVNNREIHTLDGTSTQLEDGDEVAVIPAIAGGASVEGNGATPA
                     YRPMTPEQVQRYSRHIIMSQVGSVGQRKLLDSKVLIIGAGGLGSPAALYLAAAGVGTI
                     GLVDFDTVDLSNLQRQLLHHTSDVGRPKVDSARDTIASYNPDVTVIPHPVPLTSDNAF
                     EIFEPYDLILNGVDNFPARYLANDAAYLLEKTLVDGGIFLFEGLASVFKPGKGCYRCL
                     FPTPPPPGMVPSCAEAGVLGVLPGIIGSIMAIEAIKLILDIGEP"
BASE COUNT          431 a          788 c          762 g          392 t
ORIGIN      
        1 gcggtcgccc gctccagagc agcgagatcg cggcgcgcca agccatcccc gagcagtacc
       61 tcgaccagct tctgacgaca ctgagacgcg ccggcctcat tcgcagcgtt cgcggcccac
      121 agggcggaca tgcgctcctg cgccggccgg aggagatccg cgccagcgag gtcgtgatcg
      181 cgatggaggg gagcctcgac ccaattgcct gcctggagcc gggctcgccg tgcacgctgc
      241 aaggcggctg cgcccaacgg tcgatgtggg aagacgtccg cgccgcgatc ctgggggttc
      301 tcgatgcaac gaccatcgcg gatctgcgcg atcgggcact ggcccccacc gccacccgtt
      361 acgtgatcta gcgaggagct ccgggccatg agcgacagga tagccttgga gggaagggca
      421 ctcatcgccg attcggcgct ggacctgatt ggcaacacgc cgatcgtccg gttgcatcgc
      481 gtcgtgccat cgggctccgc cgaggtgctg ggcaagctcg agtcaatgaa cccggctggc
      541 tccgtgaagg atcgcatcgc cctcgccatg gtgagcgaag ccgagcgagc cggaaggctc
      601 cacccgggcg atacgattgt ggagccaacg agcggcaaca cgggcatcgg cttggcgatg
      661 gtggcggccg tcaagggcta tcgcctcatc ctcaccatgc cggaggacat gagcgtcgag
      721 cgacgcaagc tgctcgcccg ctacggtgtc gaattcgtgc ttacgcctgc gatcgaggga
      781 atgacaggcg ccgtgttcac agcgcaggaa ctggccgaga agcacggata cttcatgcct
      841 cagcagttcg agaacccggc aaaccctgag gtgcatcgcc gtaccacagc acgcgaggta
      901 ctcgaggcga cggacggcca gctcgatgca ttcgtcgctg gggtcggcac cggcggcacc
      961 atcacgggcg tcggtcaggt gctcaaggag cgcgtccccc acgtgctggt gataggcgtg
     1021 gaaccggcgc gctccccggt actgcaagga ggccgcgccg gactgcatgc tatccaaggt
     1081 attggggcga gcttcgtccc cggcgtgctc gatcgctcgg tcatggacga agtcatgagc
     1141 gtgagcgacg aggccgccct ggagatgatg cgtcggctca cccgcgagga gggcctgctg
     1201 gtcggtgtat cggccggcgc gaacgtggaa gctgcgatcc aggtcgccaa gcgcctcggg
     1261 ccggggaagc gcgtcgtgac ggtccttccc gataccggcg agcgctacct gagcatgccg
     1321 gcctagggcg ggttacaatt accagtgtgg acgagcgagc acgctcgccc accaatcgtg
     1381 gtcgcgggag gccacacggc ggaggcaaag gcaatgtcca tcaacgtcta cattccagca
     1441 ccgttccggc ggctcaccgg caatcgaagt agtgtgcact cgtccgccgc cgacgtgaat
     1501 tcgctcctgg acgatctcga taggcagttt ccggggtttc gctcgctcct ttgcaacgaa
     1561 cgcggcgagg taccgaccca catcaacatc tacgtcaaca atcgggagat ccacacgttg
     1621 gacggcacat caacgcagct cgaagatggc gatgaggtcg cggtgattcc tgccatcgcc
     1681 ggcggagcgt ccgtcgaagg gaacggcgcc actcccgcgt accgcccgat gacccccgaa
     1741 caggtgcagc gctactcgcg gcatatcatc atgtcgcagg tcggcagcgt gggacagcgc
     1801 aagcttctcg attccaaggt gctcatcatc ggagcgggcg ggctcggatc gccggccgcc
     1861 ctgtacctcg ccgctgccgg cgttgggacc atcggcctgg tcgacttcga taccgtcgac
     1921 ctcagcaacc tgcagcgcca gctgctccat cacaccagcg atgtcggccg cccgaaggtc
     1981 gattcggcca gggacacgat cgcctcgtac aatccggacg tgactgtgat cccccatcca
     2041 gtgccgctca cctcggacaa tgcgttcgag atcttcgagc cgtacgatct catcctcaac
     2101 ggcgtggata acttccccgc ccgctacttg gcaaacgacg cggcgtacct gttggaaaaa
     2161 acgctcgtcg atggcggcat cttcctcttc gaagggctcg cttccgtgtt caagcccggc
     2221 aaggggtgct accgatgcct cttccccaca ccgccaccac ccggcatggt tccctcatgc
     2281 gccgaggccg gcgtgctggg cgtgcttccg ggcatcatcg gctcgatcat ggccatcgaa
     2341 gcgatcaagc tcatcctcga tatcggcgag cca
//
LOCUS       DATMBB010000117         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_326693, whole genome shotgun sequence.
ACCESSION   DATMBB010000117 DATMBB010000000
VERSION     DATMBB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326693"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VNL92_02530"
     CDS             <1..975
                     /locus_tag="VNL92_02530"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93616.1"
                     /translation="ERGRELFLRLAETTDVVIESSPPGAWETLGIGWETLHRRNPRLI
                     LVSITPFGQTGPYRDYAGGDLIAQAMGGLMYINGYPEDPPVRAGGRQAEYSSGIHACA
                     AALMALYGRDFTSPGEGQWIDVSMQESVAAAVQPDINFWDLRGEVKKREGNFIRGAGS
                     GVFKTKDGYLALHSGLRNWADLADWLAMDGADTSFMLDPVWDEPQTRILRRSEVDSLI
                     EQLCANRTMDELADEGQRRGLFTFPVFNAEQVAHDKQMAARHWFIDLPAPGGQGTLRY
                     PGPPVRLHATPYVIRRPAPRLGEHNAEVYGSLGVAPEDLPDLRKAMAI"
     gene            1000..>2129
                     /locus_tag="VNL92_02535"
     CDS             1000..>2129
                     /locus_tag="VNL92_02535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93617.1"
                     /translation="MHDDPERRGPLSDVRVLEFSWFGAGPIAVRLMADHGAEVIRVES
                     EARIDALRTAPPKPQGMENNINASGYFNNFNPGKMSLSLNLSRPEAQQICLRLAERAD
                     LVVDNFKAGVMARWGLGYDDLRKVNPQIIVVSMPAVGMEGPRSYYGGFGTGIKAIAGL
                     QAISGYPDRIPACPPGAYPDYCINPGHALVAMMSALYHKRRTGQGQFIEVAQYESTTC
                     VSDTALLDYFANARIQGPAGNSDRVAAPHDVYACAGDDRWIAIAVYDDAQWLALRRAM
                     GEPAWAADPSLDSAEGRRTHSARVNEGIAAWTKGHVAEDLFALLQQHGVPAGVCQTNE
                     DLQSRDPHLRERGYYATLEHAEVGPSMYDNPPFRLTTTPGASR"
BASE COUNT          382 a          734 c          677 g          336 t
ORIGIN      
        1 gagcgcggtc gcgagctctt cctccggctc gccgaaacga ccgacgtcgt catcgaaagc
       61 tcgccgccgg gcgcatggga gacgctcggc atcggctggg aaacgctgca tcggcgcaat
      121 ccacgcctca tcctcgtctc gataacgccg ttcgggcaga ccgggccgta tcgagactac
      181 gccggtggcg acctcatcgc gcaggccatg ggcggcctaa tgtacatcaa cggctacccc
      241 gaggatcccc ctgtgcgagc gggcgggcgg caggccgaat actcgtcggg cattcacgcc
      301 tgcgcggccg cgctcatggc gctctacggt cgcgacttca cttccccggg cgagggacag
      361 tggatcgatg tctcgatgca ggagagcgtg gccgccgccg tccagcccga catcaacttc
      421 tgggacctga ggggcgaggt caagaagcgt gaagggaact tcatccgcgg agccgggagc
      481 ggcgtgttca agacgaagga cggctacctc gccctgcata gcggccttcg caactgggcc
      541 gatctcgcgg attggcttgc gatggacggc gcggacacca gcttcatgct cgaccccgtt
      601 tgggacgagc cgcagacgcg catcctccgc cgaagcgagg tcgatagcct catcgagcag
      661 ctctgcgcga accggacgat ggatgagctc gccgacgagg gccagcggcg cgggctcttc
      721 acgttccccg tgttcaacgc cgagcaggtg gcgcacgaca agcagatggc cgcacgacat
      781 tggtttatcg accttcctgc cccgggggga caaggcacgt tgcgctatcc ggggccaccc
      841 gtccgcttgc acgccacgcc ctacgtcatc cggcggccag cgcctcgctt gggcgagcac
      901 aacgccgaag tgtatggctc gctcggcgtg gcaccggagg acctcccgga cctgcgcaag
      961 gcgatggcca tctgaacacg gcagcacatg gaggcccgta tgcatgacga tcctgagcgc
     1021 cgaggcccgc tgagcgacgt tcgcgtcctc gagttctcct ggttcggggc gggacccatc
     1081 gccgtgcggc tgatggccga ccacggcgcc gaggtgattc gcgtggaatc ggaggcacgc
     1141 atcgatgcgc tacggacagc gccaccgaag ccgcaaggca tggagaacaa catcaacgcc
     1201 agtggctact tcaataactt caaccccggc aagatgagcc tctcgctcaa tctatcgagg
     1261 ccggaggctc agcagatctg ccttcgcctg gcggagcgtg ccgacctcgt ggtcgataat
     1321 ttcaaagccg gcgtcatggc gcgctggggc cttggctacg acgatctccg caaggtcaac
     1381 ccgcagatca tcgtcgtcag catgcccgcg gtcggcatgg aaggaccgcg cagctactac
     1441 ggaggcttcg ggaccggcat caaggctatc gcaggcctcc aggccatcag cggctatccg
     1501 gaccgcatcc ccgcctgccc tcctggggcc taccccgact actgcatcaa tcccgggcac
     1561 gcgctcgtcg cgatgatgtc ggcgctctat cacaagcgac gcacgggcca gggtcagttc
     1621 atcgaggtcg ctcagtacga gtcgacgacc tgcgttagcg atacagccct gctcgactac
     1681 ttcgcgaatg ctcgcattca gggccctgct ggcaattccg accgtgtggc cgccccgcat
     1741 gatgtctacg cgtgcgcggg ggacgaccga tggatcgcga tcgcggtcta cgatgacgcg
     1801 cagtggctcg ccctccgccg cgccatgggc gagcccgctt gggccgccga cccgtccctc
     1861 gactcggcgg agggacggcg aacgcatagc gcccgcgtca acgaaggcat cgcggcatgg
     1921 accaaaggtc acgtcgccga ggacctgttc gcgctgctcc aacagcacgg cgtccccgcg
     1981 ggcgtatgcc agacgaacga ggacctgcaa tcccgtgacc cacatcttcg cgaacgcggc
     2041 tactacgcta ccctcgagca cgccgaggtt gggcccagca tgtacgacaa cccgccattt
     2101 cgtctcacga ccacaccggg tgcctcccg
//
LOCUS       DATMBB010000118         2258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_247843, whole genome shotgun sequence.
ACCESSION   DATMBB010000118 DATMBB010000000
VERSION     DATMBB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247843"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VNL92_02540"
     CDS             complement(<1..939)
                     /locus_tag="VNL92_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93618.1"
                     /translation="MGERLMANGREDELSQQEKYLLFAVVSLALLVSSMQFSMVSVAL
                     PELIDGLGAPLRWVGWVITIFTLAQAVSMPVAGKLSDELGRRTVFAGGLTIFAVASIA
                     CAFAPNVYVLIAARAIQGLAGGSLMPSAYGIVGDTFPGSRAQALGLISSIFPVGSVLG
                     PNIGGVIVDHLGWRWTFGLNGPIGAIVVVAILLVMRRGGQERRKRASIDYAGAGLLAL
                     GVGGLIYSLTELGQTDTDPNYIIVAAGIVTAIVGLVLFLRQESRSADPVVDLTLLRRK
                     EFAFVNTLNFFYGVSVFGLFSFIPLYAESAYGMSSSE"
     gene            complement(1095..1436)
                     /locus_tag="VNL92_02545"
     CDS             complement(1095..1436)
                     /locus_tag="VNL92_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWO93619.1"
                     /translation="MITKMESTHYHVQDMDRAVRYYEGTLGLPLKFRDGDEWAEFKLE
                     GGKFALERNHHDTAPAPGPTVTFRTDDLDALLNALTKAGHSVGEPEQRPYGRLVETYD
                     TEGNVLMLLGD"
     gene            complement(1433..1900)
                     /locus_tag="VNL92_02550"
     CDS             complement(1433..1900)
                     /locus_tag="VNL92_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HWO93620.1"
                     /translation="MATTKAEALAELDDGYEKLRRAIMGLTDAQRNETWLGSWSVREM
                     LAHVNGWHEEMTAALQRLARGERPTPEGVDYGDADAWNARFVEGRQGASVQDEIDRFD
                     RTHHAFRAAADAVPEDRFGEGKTVNRLVENTGARHYREHLEQLEQWIVTGKAQ"
     gene            1992..>2258
                     /locus_tag="VNL92_02555"
     CDS             1992..>2258
                     /locus_tag="VNL92_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO93621.1"
                     /translation="MAKGERPKLPNDPPAPGDPGEPLVVATSRGPIACRYHPGRAGAG
                     GVVLIGGTDAGLQGPADWIYPTLAADLSELGISSLRVDYRVKRAP"
BASE COUNT          410 a          741 c          721 g          386 t
ORIGIN      
        1 ctcgcttgac gacattccat acgccgactc ggcgtagagc ggaatgaatg agaagagacc
       61 gaacacggac acgccgtaga agaagttgag cgtgttcacg aacgcgaact ctttgcgccg
      121 aagcagcgtg aggtcgacca cagggtcggc gctgcgagac tcctgccgaa ggaacagcac
      181 gagcccgacg atggcggtga cgattcccgc tgccacgatg atgtagttcg gatcggtgtc
      241 tgtttgaccg agctcggtga gcgaatagat gagcccccca acgccgaggg cgagcaggcc
      301 cgcgccggcg taatcgatcg acgcacgttt gcgccgctct tggccgcccc ggcgcatcac
      361 cagaagaatg gcgacgacca cgatggcacc gataggccca ttgagcccga aggtccaacg
      421 ccacccgaga tgatcgacga tgacgcctcc aatgttcggc ccgaggacgg agccgacggg
      481 gaagatgctg gagatgaggc cgagcgcttg ggcacggctc ccagggaacg tatcgccaac
      541 gatcccgtat gccgatggca tcaggctccc gcccgccagg ccttggatcg cgcgcgccgc
      601 gatgagcaca tagacgttcg gcgcgaacgc acaggcaatg gaggcgaccg cgaagatggt
      661 gagcccgccg gcgaagacgg tccgtcgtcc aagttcatcg ctgagcttcc cggcgacagg
      721 catggatacc gcttgggcga gcgtgaagat cgtgatgacc cagccgaccc aacgaagcgg
      781 cgcacccagt ccatcgatga gctccgggag agccaccgac accatggaga actgcatgga
      841 cgaaacgagc agtgcgaggg agacaaccgc gaagagaaga tacttctcct gctgactcag
      901 ttcatcctcg cgcccgttgg ccatcaaacg ctctcccata cgtgaaggtt aacatcgtca
      961 agggtaactg cttccagtcc tttcgtcgag ccaagatgtt gttgggacag ggagcttctt
     1021 ccggccgctt ccgggggacg cgcgacggtc gctgcccagg acaaggagtc cgcgggcgca
     1081 accgcgtgtc cggctcagtc gcccaggagc atgaggacat tgccctcggt gtcgtacgtc
     1141 tcgacgagcc gcccataggg ccgctgctcc ggctcgccca cggagtgccc tgccttcgtg
     1201 agcgcgttca gcagcgcgtc gaggtcgtcc gttcggaagg tcaccgtggg gccaggcgcg
     1261 ggcgcggtat cgtgatggtt gcgctcgagg gcgaacttgc cgccttccag cttgaactcg
     1321 gcccactcat cgccatcgcg aaacttcaga ggcaaaccca gtgtgccttc gtaatagcgc
     1381 accgcgcggt ccatgtcctg cacgtggtaa tgggtgctct ccatctttgt gatcattgtg
     1441 ccttccccgt gacaatccac tgctcgagct gttccaggtg ctcacggtag tgacgggcgc
     1501 cggtgttctc aacgagccga ttcacggtct tgccctcgcc gaaccggtcc tcggggacgg
     1561 cgtcggcggc ggcccggaac gcatggtgtg tgcgatcgaa tcgatcgatc tcgtcctgga
     1621 cagaagcgcc ctgacgcccc tcgacgaagc gtgcattcca tgcatcggcg tcgccgtagt
     1681 ccacgccctc aggcgtaggg cgctcgccgc gggcgagccg ctgcagcgcc gccgtcatct
     1741 cctcgtgcca gccgttgacg tgcgctagca tctcgcgcac gctccacgac ccgagccagg
     1801 tctcgttgcg ctgtgcgtcc gtcaagccca tgatcgcacg gcgcagcttc tcgtagccgt
     1861 catccagttc cgccagtgct tccgccttgg tcgttgccat gccatctcct ccactcgtgt
     1921 cgcttcgcat gcctcgcgag ggtattgtac ggaggtcggg caagcgcgac gcttgagtgg
     1981 gagtactgac gatggcaaag ggcgagcgtc cgaagcttcc gaacgatccc ccggcaccgg
     2041 gcgatccggg cgaaccgctc gtcgtcgcca catcgcgcgg acccatcgcc tgtcgctacc
     2101 atcccgggcg ggccggcgcg ggaggggtcg ttctcatagg cggcacggat gccggcctcc
     2161 agggacccgc ggactggatt tacccgacgc tggccgcgga cctcagcgag ctcggcatct
     2221 cctcgctgcg ggtcgactat cgcgtcaaac gggcgccc
//
LOCUS       DATMBB010000119         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_526530, whole genome shotgun sequence.
ACCESSION   DATMBB010000119 DATMBB010000000
VERSION     DATMBB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526530"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1530)
                     /locus_tag="VNL92_02560"
                     /pseudo
     CDS             complement(142..1530)
                     /locus_tag="VNL92_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(1527..2342)
                     /locus_tag="VNL92_02565"
     CDS             complement(1527..2342)
                     /locus_tag="VNL92_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93622.1"
                     /translation="MIDVGVDSRTLAGLSHMIPEGRKIRETPDEVSPSWVLVYRDLCE
                     WAALYELICYRSFASDTLLVRDGLLRSKLFRGEYFIAFRKRIEEAIDRIQTRRKVYLV
                     GVAKHSKVLARYNLARALEGTIAAGAPRFVKVPRSLEAKAYVWPEYARGAESEGGGGE
                     APKFVAGDMYFVRFGDRSGDPLWTVDIFSRQVGQAAEIFGYLLADAIDGFPVPFYPRC
                     LQRAHEFAQIVDFDLDILQDEVIGAVRGLLSDREVPALEALRLQGDVSGRRYE"
BASE COUNT          424 a          827 c          809 g          438 t
ORIGIN      
        1 tccgcctcct cgggggcgtc gtctacatcg tcggcggtgt cgaagggaac tccgactgca
       61 tgatggacgc cggcgcgctc gccatcacgc agccggtgcg gagaaggtca cgggcacgtc
      121 atcggacgcg cgcgctaaca ctttcaccca cgcgacgtac ggcccttcac cggaactgcc
      181 gccgctgccg aggaccgact gcgcctcctc gaccacggcg atcacgggaa tcgtctgcgg
      241 ctgtgccttg gtgaactcct cctggttgtg gtcgaagatg cgccgaagga tcagccccga
      301 gagcacgagc gccggcgtcc cccgcatctg cgacacgtcg atcacgcaga gctttccatc
      361 gcgcagtgcg gccaggagca tgtccatcat ctggctcgct gggtcgtgta gcatccggac
      421 gatcgtcgtc atgttcgccc gcgccgcgac catctcggcg tcctgacccg gctcgaggcg
      481 cagcagctgc ttgatagtct ctccgtcggc agcgttgccg tcgcggtgaa cgagatcgac
      541 gagctggtgc caatcggcgt cgttcatgcc cttcagcttg cggacgttct gttggtcctg
      601 cttctcgggc gggagcgcga tcgagatgac gtcgccggga cgcaggcggc gaatgtccaa
      661 gcggatgtcg ccggcgacga acgacttgta aaacgggctc gggccgtcct tgggggtgaa
      721 gacaacgatc ttgtcctgga gaacgggcac gtcgcagagg cccgggcggt tcttatcgtc
      781 cggccagaag tactctccgt cgggatcgaa gatcaccgtg cccaccggca cgtggcggtc
      841 ccctcgcttc ggtaccgtcg gggtggtctc gtagagatcg gagaagagca acttcacgag
      901 gttcgacttg ccgaagccgg cccgtgcaaa cacgagcgtc cttcgcgcga cgaggtgcag
      961 cacctcgaac ttcggaacca cgcatggcgc cttgacctgc atccagggct cgggcttgag
     1021 ccgagcatcg ccgctggcgt agatgaactc gcccagcgcg aagtgtccga gctcggccgc
     1081 gttctggatg ttgtggcccg cgacctctcg aaggagatcc tgaccgagga aggcgacctt
     1141 actcccgacg tgtggcaggc gccggtggga tgcggcgaac accacctgtt cgcccacgtt
     1201 tctcaggacg ccgaggacgc gcatgtggac acggtacttc aggtactgct cgcgcaggtc
     1261 ctctgggatg gggcgctccg ctgcgaccgc gcggatgccg tagtcctcgc caaccgacga
     1321 cgcgagccgg ccttcggacg agatcgacgt gatccgcccg agcaccgcct cgtcttcgtc
     1381 ttcgagctgc accacgagga actggccgtg catcggcgcg ctttgaaact cgttgcggta
     1441 cggcaggacg aggtcggcgt gaaactcgag gcccccctcg ctgaaccccc ggaagatgcc
     1501 gacggtcgtt tctctcgtga agagtttcat tcgtacctcc ggccggacac gtcaccctgg
     1561 aggcggagag cctcgagggc aggaacctct ctgtcgctca gcaatccgcg aacagcgccg
     1621 atcacctcgt cctggaggat gtcgagatcg aagtcgacga tttgggcgaa ctcgtgtgcg
     1681 cgctggaggc aacgtggata gaacggcaca gggaagccat cgatcgcatc cgccagcagg
     1741 tagccaaaga tctccgccgc ctgaccgacc tgcctgctga agatgtcgac ggtccacagc
     1801 gggtcgccgc ttcgatcgcc gaagcggacg aaatacatgt cgccggcgac gaacttgggg
     1861 gcctcgccgc cgccgccctc gctctcggcg cccctcgcat actcgggcca gacgtaggcc
     1921 ttcgcctcga gcgaccgcgg gaccttcacg aagcgagggg cgcccgcggc gatcgtgcct
     1981 tcgagcgccc tcgctaggtt gtaccgcgcg agcaccttgc tgtgcttcgc gacgccgacc
     2041 aggtacacct tgcgccgtgt ctggatccgg tcgatggcct cctcgatccg cttgcggaac
     2101 gcgatgaagt actcgccccg aaacagcttg ctgcggagga gaccgtcacg gaccagcagc
     2161 gtgtcgctgg caaagctgcg gtagcagatg agctcgtaga gggccgccca ctcgcacaga
     2221 tcgcggtaga cgaggaccca cgacggcgag acctcgtcgg gcgtctcccg gatcttgcgg
     2281 ccctccggga tcatgtggct caagccggcg agggttctgg aatcgactcc gacatcgatc
     2341 attacccgcc caagcgcctc aactttttga cgcttcgctt gggctcgacc cccatgtcca
     2401 tctcgtgtgt atcgatgggg tctacgagcg ctgtgggggt ggcacgctcg cgctccacga
     2461 cgcgggcaca ccctccacgc tcgaggtgga gacggtag
//
LOCUS       DATMBB010000120         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_471035, whole genome shotgun sequence.
ACCESSION   DATMBB010000120 DATMBB010000000
VERSION     DATMBB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471035"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VNL92_02570"
     CDS             complement(<1..579)
                     /locus_tag="VNL92_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93623.1"
                     /translation="MKRPLEGIRILDFTIFQQGGYATVILADLGAEIIKIERPFLGDF
                     GRFLDIFRAGEQTVSAYFLAHDRGKRSVTLDLSKPVACEVIARLVADVDVVTHNFRPG
                     VMERLGLGYDDLVKFNPKLVYAAASGWGTEGPSALRPAFDIAAQAQSGLIAATGDEAG
                     HPVPAGAAIADHVGAVNLALAVVAALLRRERTG"
     gene            complement(702..1652)
                     /gene="ispH"
                     /locus_tag="VNL92_02575"
     CDS             complement(702..1652)
                     /gene="ispH"
                     /locus_tag="VNL92_02575"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWO93624.1"
                     /translation="MTTDGQPPTRKLLIAAPRGFCAGVVRAYDILDEVLRREPPPVYC
                     FHEIVHNRYVVRSFEERGAVFVDDVELVPDGARLIFSAHGVSPAVRERAAAKRLRIID
                     ATCPLVTKVHLEARKAARDGFDVVLIGHEDHEEIEGTRGEAPDRTVVVGSKEDVERLA
                     PENADRVFVVTQTTLSVDDTRDVIDEIRRRAPNAEVRNDICYATENRQQAVRTIASRA
                     QLVLVVGSQNSSNSNRLREVAASAGARAYRIDDASEIRDEWLEGVETIGLTAGASVPD
                     VLLDRVIEYLRRRGVSDVEEVRVADETQVFALPEELAPRG"
     gene            complement(1728..>2443)
                     /locus_tag="VNL92_02580"
     CDS             complement(1728..>2443)
                     /locus_tag="VNL92_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWO93625.1"
                     /translation="SAAWPNLVAGANKRETHLRNTNLGRDWSAYLLTDIVLARAGDPC
                     PHCEAALTTSRAIEMGHIFQLGTKYSEQLGATFLDAQGRPQPVIMGSYGIGIERLLAG
                     VIEANHDERGIIWPAELAPYDVHLVALNPDRPGVTETTENVLADLEAAGLRVLVDDRD
                     ESPGVKFNDADLLGMPVRATVSPRNVEAGVVELRMRRSGETMVVPMEDALEACRSMLA
                     GHREATRDPALSPASQAAG"
BASE COUNT          389 a          796 c          856 g          402 t
ORIGIN      
        1 gcccgtcctc tctcgccgga gcagggccgc gacgaccgcg agggcgaggt tcacggcgcc
       61 aacgtggtcg gcgatggcgg cgcctgccgg tacgggatgc ccggcctcgt cgccggtggc
      121 ggcgatgaga ccgctctggg cctgggccgc gatatcgaag gcggggcgaa gcgccgaagg
      181 accttcggtg ccccagcccg aggcggctgc ataaacgagc ttagggttga acttcacgag
      241 gtcgtcgtag ccgaggccca gacgctccat gacgccgggg cggaagttgt gcgtgaccac
      301 atcgacatcg gccaccagcc gggcaatcac ctcgcacgcc acgggtttcg agaggtcgag
      361 cgtcacgctg cgcttgccgc ggtcgtgggc gaggaagtag gccgagaccg tttgctcgcc
      421 cgcccggaag atatcgagga agcggccgaa atcgccgagg aagggacgct cgatcttgat
      481 gatctccgcg cccaggtcgg caaggatgac ggtggcgtag cctccctgct ggaagatcgt
      541 gaagtcgagg attcggatgc cctcgagcgg acgcttcatc tctcactcct gccgcgactg
      601 cgcgcagtct agcgtccact ccggcgattc tcaagcccag ggagcaccga gagggacggg
      661 agagcctgat catggcgctc ctccgtcgca gggcagaagc cctagccgcg aggcgcgagc
      721 tcctcgggca gggcgaagac ttgggtttcg tccgcgacgc gaacctcttc gacatcgctc
      781 acgccgcggc gccgcaggta ctcgatcacg cgatcgagca ggacgtcggg caccgaggcg
      841 ccggcggtga gcccgatggt ctcgacgcct tccagccact cgtcgcgtat ctcggaggca
      901 tcgtcgatgc ggtaggcgcg ggcgccggcg ctcgctgcga cttcgcggag gcggttcgag
      961 ttggagctgt tctgcgaccc gacaacgagg acgagctggg cccgactggc gatggtccga
     1021 acggcctgct gacggttctc cgtcgcgtag cagatgtcgt tccgcacctc ggcgttgggc
     1081 gcgcggcgac ggatctcgtc gatgacgtcc cgcgtgtcgt ccaccgaaag cgtcgtttgc
     1141 gtcacgacga agacgcgatc ggcgttctca ggcgccaggc gttcgacatc ttccttcgag
     1201 cccacaacca ccgtgcgatc gggcgcctcc cctcgcgtgc cttcgatctc ctcgtggtcc
     1261 tcgtggccga tgaggacgac gtcgaagcca tcgcgcgcgg ccttgcgcgc ctcgaggtgc
     1321 accttcgtga cgagcgggca tgtggcatcg atgatgcgca gccgcttcgc tgccgcgcgc
     1381 tcgcggacgg caggggagac gccgtgcgcg ctgaagatga gacgcgcgcc atcggggacg
     1441 agctcaacgt cgtccacgaa cacggctccg cgctcctcga aggagcggac aacgtaccgg
     1501 ttgtgcacga tctcgtggaa gcagtacacg gggggcggct ccctccgtag cacctcatcg
     1561 aggatgtcgt aggcccgcac gacgccggcg cagaacccgc gtggggccgc gatgagcaac
     1621 ttccgtgtcg gaggttggcc gtcggtcgtc atcggattca gtatacgcgg tcgggccaag
     1681 ggcagaggcg tgctgatgca ggaaaggaag aggcgtcgac cccagcttca gcccgccgcc
     1741 tggctcgcgg gcgacagcgc cgggtcccgg gttgcctccc tgtggccggc gagcatcgat
     1801 cggcaggcct cgagggcgtc ctccatggga acgaccatgg tttcgcccga acgtcgcatc
     1861 cgaagctcca ccactcccgc ctcgacattc cgtgggctga ccgtggcgcg gaccggcatt
     1921 cccagaaggt cggcgtcgtt gaacttgacg ccgggcgact cgtcgcggtc atcgacaagc
     1981 acacgtaagc cggcggcttc gagatcggcg agtacgttct cagtcgtctc cgtaacgccc
     2041 ggcctgtcgg ggttgagcgc gacgaggtgc acgtcgtatg gagccagctc ggcgggccag
     2101 atgatgccgc gctcatcgtg gttggcctcg atgacgccgg cgagcagccg ctcgataccg
     2161 attccatacg agcccatgat cacaggttgc ggacgtcctt gggcgtcgag gaacgtcgcg
     2221 ccgagctgct cgctgtactt ggttcccagc tggaagatgt gccccatctc gatggcacgg
     2281 ctcgtcgtta gggcggcctc gcaatggggg caggggtcgc ccgcgcgcgc gaggacgatg
     2341 tcggtcagaa gatacgcgct ccaatcgcgg ccaaggttgg tgttccggag gtgggtctcc
     2401 cgcttgttcg cgcccgcgac gaggttcggc cacgccgccg att
//
LOCUS       DATMBB010000121         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_136081, whole genome shotgun sequence.
ACCESSION   DATMBB010000121 DATMBB010000000
VERSION     DATMBB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136081"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VNL92_02585"
     CDS             complement(<1..1733)
                     /locus_tag="VNL92_02585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HWO93626.1"
                     /translation="MFLFVLILSRPTVAAVSTPLSLDELARGSAGVIVGDVQQVTGVE
                     TRERDIVTLVKVRVVDVLRGQVTRPLITLKEDGGKVGARIKVVYGSPRFRVGERVVLF
                     LERRPDGTLRTRDLALGKFSLVGDGEDAQAIRDLSDTMLVLAPGQLDPPRALSLRELR
                     LAVRDGSDGSAIPLPHAAPPLPFTVPAEAVDPTVAREEADGQVEYRGRFFEVDEGFPI
                     GMFIDEVNDSFLGPGANRLVWHAAMAAWTNVQPASIVLWDAGGLPPPEGPLLSFADNP
                     ASAGCSGVSGVGGWVANDAAEVKRFGGNTYVRITGAFGLVYRGRPECNGLYTRCNAAE
                     VATHELGHAIGLLHSRNPDATMNSAPDFDCRCGAVREDDIQTLGAYYPLATPPTVTTP
                     AMLPDAVVGAPYEHHLEAIGGTGAFTWAFADDGFNPGVDDFRPGLVLSEDGVLSGAPV
                     YSDTGGPEYIERGLIRATDTNGDFHTKRFFIRIYPAGITTTTATSTTTSTAVAPVVDG
                     PIPCETTTTTIPPSPCDLADGFPGVACKAAEGERLVRTFPRRRAKAGKLIRQVASRAE
                     RARVEIARKRG"
     gene            complement(1817..2155)
                     /locus_tag="VNL92_02590"
     CDS             complement(1817..2155)
                     /locus_tag="VNL92_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93627.1"
                     /translation="MTGELTGEVHDLDPGCGDGVVVKFRRKRNQMGSPILPDITIANG
                     AVSPQQYGIELNDIAIDETIRFIIEARANTGCDATFLDPLIQVLPPEHPNVGPIPNAQ
                     PIFNPEVESD"
     gene            complement(2152..>2278)
                     /locus_tag="VNL92_02595"
     CDS             complement(2152..>2278)
                     /locus_tag="VNL92_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HWO93628.1"
                     /translation="DDWLIDYNEQRPHDALGRVPPARFYPRPLPTPEESRNAVCP"
BASE COUNT          400 a          751 c          728 g          399 t
ORIGIN      
        1 ctgccgcgct ttctcgcgat ctcgacacgg gcgcgctcgg cgcgacttgc cacctgcctg
       61 atgagtttcc cggccttcgc ccggcgtcgc gggaacgtcc gcaccaggcg ctcgccttcg
      121 gcggccttgc aggcgactcc cgggaaccca tccgcgagat cgcacgggga cggtgggatg
      181 gtggtggtcg tcgtctcgca ggggatgggc ccatccacca cgggtgcaac ggccgtgctc
      241 gttgtcgtgc tggtagccgt ggtcgttgtg atacccgcgg gatatatccg gatgaagaag
      301 cgcttcgtgt ggaaatcccc attcgtatcc gttgctcgga tcagcccccg ctcgatgtac
      361 tctggtccgc cagtgtcgga gtagacggga gcacccgaca gcacaccgtc ttcgctgagg
      421 acgagccccg gtcgaaagtc gtcgacgccc ggattgaagc catcgtctgc aaacgcccac
      481 gtgaacgctc ccgtgccccc gatggcctct agatgatgtt cgtatggggc acccacgacg
      541 gcgtccggga gcatggccgg ggtggtgacc gtcggcggcg tagcgagcgg atagtaggcg
      601 ccgagtgtct gaatatcgtc ctcgcggaca gcgccgcaac ggcagtcaaa gtctggcgcc
      661 gaattcatcg tggcgtccgg gttccgcgag tgtagcagac cgatggcatg tccgagctcg
      721 tgtgttgcaa cctcggcggc gttgcatcgc gtatagagcc cgttgcattc tgggcgaccc
      781 cggtacacga gcccgaacgc gccggttatg cgtacatagg tgttcccgcc aaagcgcttg
      841 acctccgcag catcattggc cacccaccct ccgacgccgc tcacgccgct gcaaccagcg
      901 ctggccgggt tgtccgcaaa gctcaagaga gggccttctg gaggggggag gccaccggcg
      961 tcccagagaa cgatgctcgc cggctggaca ttcgtccacg ccgccatcgc cgcatgccag
     1021 actagccgat tcgccccagg gccaaggaag gagtcattga cctcatcgat gaacatccca
     1081 atggggaagc cctcgtccac ctcgaaaaat cgaccacgat attcgacctg cccatccgcc
     1141 tcttcgcgcg cgaccgttgg gtcaaccgcc tccgcgggaa ccgtaaacgg cagcggaggt
     1201 gcagcgtgcg gcagaggaat ggcgctgcca tcgctgccat ccctcactgc cagacgcagc
     1261 tcacgcagcg agagcgcgcg cggtgggtcg agttggccgg gcgcaaggac aagcatcgtg
     1321 tcagaaagat cgcggatcgc ctgggcgtcc tcaccgtcgc ctaccaggct gaacttgccg
     1381 agcgcgaggt cgcgcgtccg cagggttcca tcggggcggc gctccaggaa gagcacgacg
     1441 cgctcgccga cgcggaagcg gggcgagccg tacacgacct tgatgcgtgc acctaccttc
     1501 cctccgtcct ccttcagcgt gatcagcggg cgggtgacct gaccacgaag gacgtcgact
     1561 acgcgtactt tcacgagggt cacgatgtca cgctcgcgtg tctcgacacc cgtaacctgc
     1621 tgcacatccc cgacgatgac gcccgccgac ccccgcgcca gttcatcgag gctcagcggc
     1681 gttgaaacag ctgcgaccgt cggacgcgac aggatcagta cgaagagaaa cacgcaacgg
     1741 cataacgggt ggagcggcag gctgaccgcc ctgcccgctg caggccgtcc gcgtcccgcc
     1801 cttgggttcg cgctggtcaa tccgactcga cctcgggatt gaagatcggc tgcgcattgg
     1861 gtatcggccc gacgttcggg tgctcgggag gaagcacctg gatcaaggga tccaggaatg
     1921 tcgcgtcgca tcccgtatta gcccgcgctt cgatgatgaa tcgtatcgtc tcatcgatcg
     1981 cgatgtcgtt gagctcgatg ccgtattgct gtgggctcac ggcgccgttt gcgatggtaa
     2041 tgtccggaag gataggactg cccatctgat tgcgcttccg gcggaacttc acgacaaccc
     2101 cgtcaccgca tcccggatca agatcgtgga cctcgcccgt aagctccccc gtcaaggaca
     2161 cacggcattc ctagactctt ccggggttgg gagcggccgg ggatagaagc gcgccggagg
     2221 cactcggccc agcgcgtcgt gcggccgctg ctcgttgtag tcgatgagcc agtcgtcc
//
LOCUS       DATMBB010000122         3410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_312402, whole genome shotgun sequence.
ACCESSION   DATMBB010000122 DATMBB010000000
VERSION     DATMBB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312402"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..649)
                     /locus_tag="VNL92_02600"
     CDS             complement(107..649)
                     /locus_tag="VNL92_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWO93629.1"
                     /translation="MATRYIVVAGGQDHEAQVSRRGGGLDIVLDGEQHRVRLVPEGPP
                     QVYLLTDSGTRHEVGVEREQREVHVVLDGRRYRIGVYTEGQRRAPGGRVERASERELE
                     GGAWTLLSPMVGLVLSVHVKEGEVVEPGDILIVIEAMKMNNEMRALRRSRVQRIHVLP
                     GDRIEQGADLITFEAADSVP"
     gene            complement(628..2142)
                     /locus_tag="VNL92_02605"
     CDS             complement(628..2142)
                     /locus_tag="VNL92_02605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWO93630.1"
                     /translation="MFRKVLIANRGEIAVRIARACRDVGVGSVAVFSEADRFALHTRL
                     ADEAHYIGPSPVGESYLSIERLLEAARTTGADAVHPGYGLLSENATFAQRCLDTGLTW
                     IGPSPEAIAAMGDKRAARRRMAAAGVPVVPGSEGVDTAEDALRAAEHVGFPLLVKAAA
                     GGGGRGMRLVRHPDELESNFHAAKAEAEAAFGDGSLYLERYLVPARHIEIQIVGDHHG
                     NYAALSERECSIQRRHQKVIEEAPSVVADASLRSRMAEASLAAARAVQYTSLGTLEFL
                     VDECGEFYFLEMNTRVQVEHPVTEMITGHDLVADQIRIAAGLPLAYEGLIEPRGWSIE
                     CRIAAEDPYNNFLPSLGRIASVRKPGGPGVRVDSSLFDGMEISPYYDSLVAKLITWGR
                     DRPEAITRMRRALREFVVVGVATNIPFHRQVMEDPDFLEGRLDTGYLDRFRFRDEMDQ
                     DALRLAMLAAVAHRFQTDERGAPRQTEPRRAAGWRQMRTLGSDSGERGWRRAIS"
     gene            complement(2158..>3410)
                     /gene="thrS"
                     /locus_tag="VNL92_02610"
     CDS             complement(2158..>3410)
                     /gene="thrS"
                     /locus_tag="VNL92_02610"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWO93631.1"
                     /translation="AWPTEEELDTYLHRLDEAARRDHRRLGRELGLFYVDPIAPGSPF
                     FLPKGALLYNLAIDFMRGLYRRYGYQEVITPEIYSTDLWKRSGHYENYIENMYFIHDE
                     DRELGVKPMNCPGHCLMFASQVRSYRELPIRYADFGRLHRFERSGVLHGLTRVRSFSQ
                     DDKHVYCSLNQVPGEIERVLEMLKTTYDAFGMGTPRYTLSLRPEKRIGADEVWDLAED
                     TLREALHSLGVTFQEVPGEGAFYGPKIDLFMDDAIGREWQLGTFQLDFNLPERFDLHY
                     AAEDGSQQRPVMIHQAIFGSIERFLGVLIEHHGGAFPLWLAPVQATVIPIADRHIDYA
                     HDVAQALREEGIERVEVDGRNERMNAKIRDAQLQKVPYMLIVGDREAEAETVSVRPRA
                     GDEEKGVPLRELTGRLRLEAEQRR"
BASE COUNT          551 a         1115 c         1146 g          598 t
ORIGIN      
        1 gggagccctc ggtggcggag cctggttcta cctccagcgc cggcctgatg ccggacaggc
       61 cgcggtggcc ggtcggtcgg gcgcggccgc gacgggcggc tgacgctcag gggacggagt
      121 cggctgcttc gaacgtgata aggtcggcgc cctgctcgat gcggtcgccg gggaggacgt
      181 ggatccgctg gacgcgggag cgtcgcaagg cgcgcatctc gttattcatc ttcatggcct
      241 cgatgacgat cagaatgtcg cccggttcga cgacctcgcc ctccttcacg tgcaccgaaa
      301 gaacgaggcc caccatgggg gataggagcg tccaagctcc tccttccagc tctcgttcgg
      361 aggcgcgctc gaccctgccg ccgggcgcgc gacgctgacc ctcggtgtac acgccgattc
      421 ggtatcggcg cccatcgagg accacgtgca cctcacgttg ctcgcgctcg accccgacct
      481 cgtggcgggt gccggaatct gtgagcaagt agacttgcgg cgggccttcg ggcacaagcc
      541 gcacgcgatg ctgttcgcca tcgagcacga tgtcgaggcc gcctccccgg cgtgagacct
      601 gcgcctcgtg gtcctgaccg cctgcaacta cgatatagcg cgtcgccacc ctcgttctcc
      661 tgaatcgctg cctagcgtcc gcatctgccg ccagccggcg gcccgccgtg gttccgtctg
      721 tcgcggcgct ccgcgctcat ccgtctggaa gcgatgagcg acggcggcca gcatggccaa
      781 gcgaagtgcg tcctggtcca tctcgtccct gaaccgaaag cggtccaggt agcccgtgtc
      841 gaggcggccc tcgagaaagt cggggtcttc catgacctgt cggtggaacg ggatgttcgt
      901 cgcgaccccg acaacgacga actcgcggag cgctcgacgc atccgggtga tagcctccgg
      961 ccgatcccgg ccccaggtga tgagcttcgc gacgagcgaa tcgtagtagg gcgatatctc
     1021 catcccatcg aagagcgagc tgtcgactcg tacgcccggt cctcccggct tccgtacgga
     1081 cgcgatgcgt cccagcgagg ggaggaagtt gttgtacggg tcctccgcag cgatgcggca
     1141 ctctatcgac cagcctcggg gctcgatgag gccctcgtac gcaagaggga ggcccgcggc
     1201 gatgcggatc tggtcggcaa cgaggtcgtg gcctgtgatc atctcggtga cgggatgttc
     1261 cacctggacg cgcgtgttca tttcgaggaa gtagaactcg ccgcactcgt cgacgaggaa
     1321 ctcgagcgtt cccaggctcg tgtactgaac cgcgcgtgct gcggcaagcg aggcttccgc
     1381 catgcgcgaa cgcaatgagg cgtccgcaac gacggacgga gcctcctcga tgaccttctg
     1441 gtgccggcgc tggatcgagc attcccgctc actgagagct gcgtagttcc catgatggtc
     1501 gcccacgatt tgaatctcga tatggcgcgc ggggaccagg tagcgctcga ggtagaggga
     1561 gccatcgccg aaggccgcct cagcttcggc cttggcggca tggaagttgg attcgagctc
     1621 gtccggatgc cggacgaggc gcatgccgcg cccccctccg cccgcagcgg ccttgacgag
     1681 caaggggaac cccacgtgtt cggcggcgcg gagcgcgtcc tccgccgtgt cgacgccttc
     1741 ggagcccggt acaacgggca cgcccgctgc ggccatgcgc cgccgtgctg cccgcttgtc
     1801 gcccatggcg gcgatggcct cggggctcgg tccgatccac gtgagtccag tatcgaggca
     1861 gcgttgcgcg aacgtcgcat tctcgctcaa caagccatac ccgggatgga cggcatcggc
     1921 gcccgtggtg cgcgccgctt cgaggaggcg ctcgatgctc agatagctct cgccaacagg
     1981 cgatggaccg atgtagtggg cctcgtcggc gaggcgcgta tggagggcga accggtcggc
     2041 ctcggagaag acggcgacac tgcccacgcc cacgtcgcgg caggcccgag cgatgcggac
     2101 ggcaatctcg ccccggttcg cgatgagcac cttgcgaaac atgtgcgtct cccggctcta
     2161 gcgccgctgt tcggcttcga ggcgaaggcg tccggtcagc tccctcagcg ggacgccctt
     2221 ctcctcgtcg ccggcacgag gccggacgga cactgtttcc gcctcggcct cacggtcgcc
     2281 cacgatgagc atgtacggaa ccttttgcag ctgcgcgtca cgaatctttg cattcatccg
     2341 ttcgttgcgc ccatcgacct cgacgcgctc gatgccctcc tcacgcaggg cctgggcgac
     2401 gtcgtgggcg tagtcgatgt gccgatcggc gatcgggatg acggtcgcct ggaccggagc
     2461 gagccagagt gggaacgcgc cgccatggtg ctcgatcagg acgccgagga agcgctcgat
     2521 cgatccgaag atcgcctgat ggatcatcac cggccgctgt tgggagccgt cctcggccgc
     2581 gtagtgcagg tcgaagcgtt cgggtaggtt gaaatcgagc tggaacgtcc cgagctgcca
     2641 ctcgcggccg atggcgtcgt ccatgaacag gtcgatcttc ggcccgtaga acgcgccctc
     2701 gccgggcacc tcctggaacg tgacgcccag gctgtgcagc gcctcgcgca gcgtgtcctc
     2761 ggcaaggtcc cagacttcat ccgcgccgat ccgcttctcc gggcgcagcg acagcgtgta
     2821 gcgcggcgtg cccatgccga acgcgtcgta ggtcgtcttc agcatctcga gcacccgctc
     2881 gatctcccct gggacctgat tcaaagagca gtagacgtgc ttgtcgtcct ggctgaagct
     2941 gcgtacgcga gtgaggccgt ggagcacgcc tgagcgctcg aagcgatgca gccggccgaa
     3001 atcggcatag cggatgggca gctcgcggta ggaacgcacc tgggaggcga acatcaggca
     3061 gtggccgggg cagttcatcg gcttcacgcc gagttcgcgg tcctcgtcgt ggatgaaata
     3121 catgttctcg atgtagttct catagtgacc cgaccgcttc cataagtcgg ttgagtagat
     3181 ttctggcgtg atcacttcct ggtagccgta gcgccggtag agaccgcgca tgaagtcgat
     3241 cgccaggttg tagagcagcg ctcccttcgg cagaaagaac gggctgccag gcgcgatcgg
     3301 atcgacgtag aagagcccga gctcgcgtcc cagcctgcga tgatcgcggc gggcagcttc
     3361 gtcgaggcgg tggagatagg tgtcgagttc ctcctctgtg ggccaggccg
//
LOCUS       DATMBB010000123         4202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_367457, whole genome shotgun sequence.
ACCESSION   DATMBB010000123 DATMBB010000000
VERSION     DATMBB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367457"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VNL92_02615"
     CDS             complement(<1..796)
                     /locus_tag="VNL92_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93632.1"
                     /translation="MPDFPLTDENRMIQQLVRRFVKEELMPLERELLGGPLGARGAFG
                     EDGALAPEKYRELIEKLRAIGLWGIDLPEEYGGNPISEMSKALLYEELAKTIVPLGIP
                     PDAPNFHMLRELTEGNEYQENEYFWPYVRGEKTSCFILTEPNAGSDAQQIETSAVRDG
                     DNWVINGTKVFISNADRADFGILMAVTDREKRARGGFTSFLVDLQDRTPGVKLARPIY
                     TIAGGYVWEVVFDNVVLPDRQRLGPVGGGFAPMQRRLGVRRLQIGSR"
     gene            complement(939..2162)
                     /locus_tag="VNL92_02620"
     CDS             complement(939..2162)
                     /locus_tag="VNL92_02620"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93633.1"
                     /translation="MVMPHALEGLRVLDFSWFGAGPMGTKVLADHGAEVIRVESIMKL
                     DGLRMAPPFPPTGEGPNLSGYYNNHNSSKLSVTLNMQRPEARDIALRLVAVSDVVIDN
                     FNPGILEKWGLAYERLVEVKSDIIQIRMPMLGLTGPHRHWIGFGMTLTGLCGFNELSG
                     FPNREPAGIGTNYPDYSSNPYHAMFAILSALHWRRRTGEGQFIELSQYESTINILHTA
                     VLDYTVNGREATRRGNRSDIAAPHGAYPCMGEDRWIAIACENDAQWAALVEHMGFPDW
                     ALEARFATLVERVANADALDDKLAEWTRDQRAEDLMWKLQRAGVPAGVVENAQDTLEN
                     DAQLRFREHFQRLEHPEAGVTYYDAPPVRLSKTPGRLRSPAPLLGQHNGYVFGELLGM
                     SEDEIRRYTEEGIFA"
     gene            complement(2164..3381)
                     /locus_tag="VNL92_02625"
     CDS             complement(2164..3381)
                     /locus_tag="VNL92_02625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93634.1"
                     /translation="MTTNQLPRALDDLRVLDLTDAKGIYCTRLFADMGADVLRVEPPG
                     GDFARRRPPFVDDLPGPERSLYHFVMNASKRSITLDIETEDGAALLRRIARSADIVIE
                     SFAPGYLDSLDLGYEDLARENPAIIVTSISPFGKGGPYEDWEGPDIVNMGLGGQLYLS
                     GFPGEPPAIPWGEQSYYQGALHGAYATMIALTYRDLTGQGQAIDVSIQECVATAMETA
                     MQFYDLQGYVRTRTGSQRRAAGFGLYPCADGFILWMAGARNFENLIEWMKSEGVDTAE
                     FETPQWSDQHYRLEHGDEFDQKFIPWGMTHTKAELAETGQAQHLPNAPIQTVAEIVSD
                     PHLRERQYYVDVDHPELGRTITYPGPPYRLQKTPARIRRAPLLGEHNWQIYREELGLS
                     LQEIATLSGAGVI"
     gene            complement(3397..>4202)
                     /locus_tag="VNL92_02630"
     CDS             complement(3397..>4202)
                     /locus_tag="VNL92_02630"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWO93635.1"
                     /translation="QNFGTMTKPLHPGLAARAGVVAADLASRGFTADEAIIDAPMGFV
                     HLFSPGQDGDPNAIPELGRPWDIVDPGISVKKYPCCFVTHNALDAILDLKAEAGFSAG
                     DVAAVEVHMPEGSAAPLIHPRPRTGLEGKFSMPYAMAAALLDGQPVLSTFEDEAVQRP
                     EAQELLRRCDLVFDPLQGELLAAYADVVVKLRDGRTLSKRSERPRGGPDAPLSREELA
                     IKYRDCARRVLSAEDAERSLTMVESLEDLSDIRELMSLLCRTSVPAAAG"
BASE COUNT          681 a         1311 c         1422 g          788 t
ORIGIN      
        1 agcgagaccc tatctgcagg cggcgcacgc cgaggcggcg ctgcatgggg gcgaagccgc
       61 cgccgacggg gccgagtcgc tgcctatcgg gaaggaccac gttatcgaat acgacctccc
      121 aaacgtagcc gccggcgatg gtgtagatgg gccgggcgag cttcacgccc ggcgtccgat
      181 cctggaggtc gacgaggaag gaggtgaagc cacctcgggc ccgcttctcg cgatcggtca
      241 cggccatgag gatgccgaag tcggccctgt cggcattgga gatgaacacc ttggtgccgt
      301 tgatgaccca gttgtcgccg tcacggacgg ccgaggtttc gatctgttgg gcgtcgctgc
      361 cggcgttcgg ctccgtgagg atgaagcagg aggtcttctc gccgcgcacg taaggccaga
      421 agtactcgtt ctcctgatac tcgttgccct ccgtcagctc ccgcagcatg tgaaagttcg
      481 gcgcgtcggg cgggatgccg agcggcacaa tcgtcttggc gagttcctcg tagaggagcg
      541 ccttcgacat ctcgctgatg ggattgccac cgtactcctc cggcagatcg atcccccaga
      601 ggccgatggc gcgcagcttt tcaattagct cacggtactt ctccggcgcg agcgcaccat
      661 cctcgccgaa cgcgccgcgg gcgccgagcg gtcctcccag caactcgcgt tcgagcggca
      721 tgagctcctc tttgacgaag cgccgcacga gctgctgaat catcctgttc tcgtcagtca
      781 ggggaaagtc gggcactacc ttgcctcctc ccaatcctct ctatcgttct tgttcgtcat
      841 cgatatcgtt gtctcgcggt cccgacgata cggttggtcg tgatcacgat gcctgcacca
      901 gtccaccgac tcggattcga tggaggcgac cgctcggctc aagcgaagat cccttcctcc
      961 gtgtaccgcc gaatctcgtc ctcggacatg ccgagcagct cgccgaacac gtatccgttg
     1021 tgctgtccga ggagcggagc agggctgcga aggcggcccg gtgtcttgga caggcgcacg
     1081 gggggagcgt cgtagtaggt gacgcccgcc tccgggtgct cgaggcgctg gaaatgctcc
     1141 cggaagcgca gttgggcgtc gttctccagc gtgtcctgcg cgttctcgac gacgccggcg
     1201 ggcacaccgg cacgctgaag cttccacatc aggtcctcgg cccgttggtc ccgagtccat
     1261 tcggcgagct tgtcgtcgag tgcatccgcg ttcgcgaccc gttcgacgag ggtggcgaag
     1321 cgggcctcga gcgcccaatc ggggaagccc atgtgctcca cgagtgccgc ccactgcgca
     1381 tcgttctcgc acgcaatggc gatccaccgg tcctcaccca tgcaggggta ggctccatgc
     1441 ggcgcagcga tgtcactgcg attgccgcgg cgggttgcct cgcgaccgtt cacggtgtag
     1501 tcgagcacgg cggtgtggag gatgttgatg gtcgattcgt actgggagag ctcgatgaat
     1561 tgcccctcgc ccgtgcgcct tcgccagtgc aaggccgaaa gtatcgcgaa catggcatgg
     1621 tacgggttgc tggagtagtc ggggtagttt gtgccgatgc ccgccggctc gcggttgggg
     1681 aagcccgaca gctcgttgaa gccgcacagg ccggtcaacg tcatgccgaa gccgatccaa
     1741 tggcgatgcg gtcctgtgag gccgagcatc ggcatgcgaa tctggatgat gtctgacttc
     1801 acttcgacga gccgctcgta cgcgagcccc cacttctcga ggatgcccgg attgaagtta
     1861 tcgatgacga catcgctgac ggcgacgagg cgcagcgcga tgtcgcgcgc ctccggacgc
     1921 tgcatgttca gcgtcacgct cagcttgcta ctgttgtggt tgttgtagta gccggagaga
     1981 ttcggtcctt cgccggtggg cgggaatggt ggggccatgc gcaggccgtc gagcttcatg
     2041 atcgactcga cacgaatcac ttcggcgccg tgatcggcga ggaccttggt gcccatgggg
     2101 ccggcgccga accaggagaa gtcgaggacg cgtagcccct cgagtgcatg tggcatgacc
     2161 atgtcagatc acccccgcgc cgctcagcgt ggcgatctcc tggagggaaa ggccgagctc
     2221 ctcgcggtag atctgccagt tgtgctcccc cagaagaggc gcgcgccgga tccgagcagg
     2281 cgtcttttgc agccggtagg gtgggccggg atacgtgatg gtacgtccga gctcggggtg
     2341 gtcgacatcg acgtagtact gccgctcgcg aaggtgcgga tcgctgacaa tctcggccac
     2401 cgtctgtatg ggcgcgtttg gcaggtgctg cgcttgccct gtctcggcga gctccgcctt
     2461 ggtgtgcgtc atcccccagg gaatgaactt ctgatcgaac tcgtcgccat gttccagccg
     2521 gtagtgctgg tcggaccatt gcggcgtttc gaattcggcc gtgtcgactc cctccgactt
     2581 catccactcg atgagattct cgaagttccg agctccggcc atccaaagaa tgaagccatc
     2641 cgcacagggg tagagcccga agccggcggc gcgccgctgg gagccggtgc gggtacgaac
     2701 atagccctgt aggtcgtaga actgcatggc tgtctccatc gccgtcgcga cgcattcctg
     2761 tatcgacacg tcgatggcct gcccttgccc agtgagatcg cggtaggtga gcgcgatcat
     2821 cgtggcgtag gcgccgtgca gcgcgccctg gtaatacgac tgctcgcccc acgggatcgc
     2881 gggcggctcc ccggggaagc ccgaaaggta caactgccct ccgaggccca tgttcacgat
     2941 gtccggcccc tcccagtcct cgtagggtcc gcctttgccg aaaggggaga tcgacgtgac
     3001 gatgatggcc gggttctccc gggccaggtc ctcgtagccg aggtcgagcg aatcgaggta
     3061 gccgggcgcg aagctctcga tcacgatgtc ggccgagcgc gcgatgcgac gcagcagtgc
     3121 cgcgccgtcc tcggtctcga tatcgagcgt gatgctgcgc ttgctcgcgt tcatgacgaa
     3181 gtggtagagg ctacgctcgg ggcccggaag gtcatcgacg aatgggggcc tccggcgggc
     3241 gaagtctcct cctgggggct cgacgcggag gacgtcggcg cccatatctg cgaagagacg
     3301 ggtgcagtag attccctttg cgtccgtcag gtcgagcacg cgcaggtcgt cgagggcgcg
     3361 cggaagctga ttcgtcgtca ttcactagtc cttggtctat cctgccgccg cgggaacgct
     3421 cgtgcggcag agcaggctca tgagctcacg gatgtcgctg aggtcctcga gcgactcgac
     3481 catcgtcaac gagcgctccg catcctcggc agacagtacg cggcgcgcgc agtcgcggta
     3541 cttgatcgcg agctcctcgc gcgaaagggg cgcgtcggga ccgccacgcg gtcgttcgga
     3601 tcgcttgctg agcgttcggc cgtcccgcag tttgacgacg acgtcggcgt acgcggcgag
     3661 cagctcgcct tggagagggt cgaacacgag atcgcagcgc cgcagcaact cttgcgcctc
     3721 cgggcgctgg acggcttcgt cctcgaacgt tgatagcacc ggttgaccat caaggagtgc
     3781 ggctgccatc gcgtagggca tgctgaactt gccctcgagg ccggtgcgcg gacggggatg
     3841 gatgagggga gcggcgcttc cctccggcat gtggacttcg acggccgcga cgtccccggc
     3901 ggagaagccc gcctcggcct ttaggtcgag gatcgcatcg agggcgttgt gcgtcacgaa
     3961 gcagcatgga tacttcttta ccgagattcc ggggtccacg atgtcccacg ggcggccgag
     4021 ctccgggatg gcattcggat cgccgtcctg cccgggcgag aacaggtgca cgaagcccat
     4081 tggtgcgtcg atgatcgcct catcggccgt gaagccgcgg ctcgcgaggt cggcggcaac
     4141 gacgccggcg cgtgccgcga ggccggggtg gaggggcttc gtcatcgtgc cgaagttctg
     4201 gc
//
LOCUS       DATMBB010000124         6236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_207025, whole genome shotgun sequence.
ACCESSION   DATMBB010000124 DATMBB010000000
VERSION     DATMBB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207025"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VNL92_02635"
     CDS             <1..512
                     /locus_tag="VNL92_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93636.1"
                     /translation="RVVDSANQPLAGATITASVEGELCAEATSFANGTYALVVPDTPE
                     RPPACDRDGASLQLAVDGVSALVVALPADGARLNVNLVRGEGMGLPSDVSVGFNSPPT
                     PGLNAAVVIGSTAATGPQIAAAASTESGMQVRAVWFFADSRWRVFVPGFLDELGTLTP
                     PLAIFVVLA"
     gene            complement(532..1560)
                     /locus_tag="VNL92_02640"
     CDS             complement(532..1560)
                     /locus_tag="VNL92_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HWO93637.1"
                     /translation="MLRHIPATVLAGAAALLSLFVMGTLGFHFVEDYPWGDAFFMTIT
                     TISTVGYREIHPLGTGGRIVASALIIGGVGVFFYFLTSMVQYTTGGAVRRVLEERRMH
                     NTIASLRGHFVLCGFGRVGEEVAADFRARSIPFVVIDSDPERAADAREARYLVIEGDA
                     TEDDVLREARIEHARGLVAATGGDAQNTYITLSARAANPDAIIIARASHRGAEAKMAQ
                     AGANQVISPYAIGGRRMALAAVQPLIVDFMETVAGPKEGDRILAELEVREGSSVLGST
                     IAEACSDLSIIVVAVARGRSDLRVGPGGDYRLAAGDRLIVMGKPAEIERFAQGSAGPR
                     TLSATSTS"
     gene            complement(1581..3872)
                     /locus_tag="VNL92_02645"
     CDS             complement(1581..3872)
                     /locus_tag="VNL92_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWO93638.1"
                     /translation="MVSRFRYSEWDGSQELPELDPDSILENLTDDLMNFGDLQHALRN
                     LLQRGMRDPMGQRMQGLRDLLQQLRQQRRQTLDRFNLSSAIDDIRKKLDDILDKERHT
                     LDERIGQAERGELGPDGRPRTDYDGPEGNPLEQDRDAMQQAIREALEKAGLQQPQGSG
                     DEGGEAGAEGQQGAEQSSPGGQQGASGSQAGSMSGSQGAGSQGQGQNADPQQFQDMLK
                     SIAERKRNFLENLPQDLGGAVKELQNYEFLDPEAQHEFQELMNQLKQAMMDTFFKDLS
                     QQIANMSPEDMQRMRDMVDALNRMLSERMAGGDPDFQSFMDEFGDMFGPNPPQSLDEL
                     IAQMQHQAAQMQSLLDSLPGDMRQQLQDLLMDKVGDPQLQAGLSELAANLEFLYPMRD
                     LRNQYPFRGDEELDLQAALQLMERMQGMDELERQLERTQYGGDIDEVDPDKVRELLGG
                     EAAETLDQMKQLLEVLEEAGYIRRKGNTWELTPRGTRKIGQKALGEIYAQLKKDAQGK
                     HAVRERGYGGDRADDTKAYEFGDPFHLHLKQTIMNSMQREGPEVPVRLQPEDFEVYKS
                     ESLTTTHTVMLVDLSWSMALRGSFQAAKKVAMALNNLITAQFTRDSLYIIGFSAYARE
                     LKADQLPYVRWDESVLGTNMHHAMMMAQKLLSKHKGGTQQIILISDGEPTAHLENGRS
                     YFAYPPSPITIRETLKEVKRATQKGITINTFMLDRNYYLKEFVNQVAKINKGRVFYTT
                     PDKLGQYILVDYVASKKKSIRGT"
     gene            complement(3874..5268)
                     /locus_tag="VNL92_02650"
     CDS             complement(3874..5268)
                     /locus_tag="VNL92_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HWO93639.1"
                     /translation="MTKKPSTIGELRDSGYKVLPVKEELRKNLIQRIRSKAPLFPGVI
                     GYEDTVIPQVENAILSGQDIIFLGERGQAKSRIIRSLVDLLDDEIPVIAGCEINDNPF
                     NPICAACRESVERHGDNVEIAWIGRDERYGEKLATPDISIADLIGEVDPIKVAEGRYL
                     SDELTIHYGMIPRTNRGIFSINELPDLAERIQVGLLNIMEERDVQIRGYRIRLPLDVF
                     VVASANPEDYTNRGRIITPLKDRYGAQIRTHYPRTVEHEIRIMEGERAPLPADYAVTV
                     PQYMKEIAAEITRLARRSPDVNQRSGVSVRASIADYESLLANALRRAIRLDEAEVVPR
                     ISDLPYVLPALSGKVELETVDEGREEQILEKLIQGAIVAVFNRYYNVTELEPVVAKFK
                     GGYSIEVGDSTPSDQYVKILREVEGMDAAIRKVGPAESAPQTAAAVEFVLEGLHLNKR
                     LNKERLAGKAQYRG"
     gene            5573..>6236
                     /locus_tag="VNL92_02655"
     CDS             5573..>6236
                     /locus_tag="VNL92_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93640.1"
                     /translation="MAAFDVRDLAPEDVEPLGDVIYEAFRDVATKHGFRPTFDSPATA
                     SLLLNTFRATELATIVTAAEGAEPVGSASMNVRSETAGIGPVAVSPRAQAGGVGRAMM
                     EELLRRADAEGCTSVRLVQAGYNVTSFSLYSKLGFVVRDVVLSLEGRIEAPETDASAV
                     REMRPEETATAAALDTRLTGFRREPDFALLRGIGPGWVWEQDGIVRGYLLSLGASGSV
                     HLG"
BASE COUNT          950 a         1975 c         2067 g         1244 t
ORIGIN      
        1 gacgcgtcgt cgattcggcg aaccaaccgt tggccggcgc taccatcacc gccagcgtcg
       61 aaggtgagtt gtgtgcggag gcgacgtcgt tcgccaacgg tacctacgca ctcgtcgttc
      121 cggacacgcc cgagcgcccg ccggcctgcg accgtgatgg ggccagcttg caattggccg
      181 tcgatggcgt cagcgcgctg gtcgtcgcgc ttcctgcgga tggcgcccgg ctgaatgtga
      241 acttggtgcg cggagagggc atgggcctgc cctcggacgt gagtgtcggg ttcaacagtc
      301 cgcccacgcc cggcctcaat gcggcagtag tcatcggcag cacggcggcc acgggtccgc
      361 agatcgcggc ggccgcctcc accgagtccg gcatgcaggt gcgagccgtc tggttcttcg
      421 ccgatagccg ctggagggtg ttcgttccgg gctttctcga tgagctcggc acgctcacgc
      481 cgcccctagc cattttcgtc gtgctcgcct gacttcaggc gcggctcccg tctagcttgt
      541 actcgtggcg ctcagggtcc gcggacccgc ggagccctgt gcgaatcgct cgatttcggc
      601 cggcttgccc atgacgatga gccggtcgcc cgctgcgagg cggtagtcac cgcccgggcc
      661 gacgcgtaga tcgctcctcc cacgcgcgac cgccaccacg atgatcgata ggtcggagca
      721 ggcttcggcg atggtgcttc ccaacaccga cgacccttct cgaacctcga gctccgccag
      781 gatccggtcg ccttccttcg ggcccgccac cgtttccatg aagtcgacga tgaggggctg
      841 caccgcggcg agcgccatgc gtcggccgcc gatggcgtac ggcgagatga cctggttggc
      901 acccgcctgc gccatcttgg cctccgcacc tcgatgcgac gcgcgagcga tgatgatggc
      961 atcggggttg gcggcgcgtg cgctcagcgt gatgtaggtg ttctgggcgt cgccaccggt
     1021 ggcggcgacg agcccgcgcg cgtgctcgat gcgcgcctct cgcagcacat cgtcctccgt
     1081 cgcgtcgccc tcgatgacca ggtagcgggc ttctcgggcg tcggcggccc gttcggggtc
     1141 ggagtcgatg acgacgaagg gaatcgaacg tgcgcggaag tcggcggcga cctcctcgcc
     1201 gacgcgcccg aagccacaga ggacgaagtg gccgcgcagc gaggcgatgg tgttgtgcat
     1261 tcgtcgctcc tcgagaactc ggcgcacggc gccgccggtc gtgtactgta ccatgctggt
     1321 caggaagtag aagaatacgc cgacgcctcc gatgatgagc gccgaggcga cgatgcgacc
     1381 ccccgtgccc agcggatgaa tctcccgata cccgaccgtg ctgatggtgg tgatcgtcat
     1441 gaagaaggcg tcgccccacg ggtaatcctc cacgaagtgg aacccgagcg tgcccataac
     1501 gaacagcgag agcagcgctg ctgctcccgc taagacggtc gccggaatgt ggcggagcaa
     1561 tcccgcctaa cctccgtgcc ttacgtcccc cggatggact tcttcttgga ggcgacgtag
     1621 tccacgagga tgtactgccc cagcttgtcg ggcgtcgtgt agaacacgcg gcccttgttg
     1681 atcttcgcga cctggttgac gaactccttc aggtagtagt tgcggtccag catgaaggtg
     1741 ttgatggtga tgcccttctg cgtggcgcgc ttcacttcct tgagcgtctc tcgaatggtg
     1801 atggggctcg gggggtacgc gaagtaggag cgtccgttct ccaggtgggc tgtcggttcg
     1861 ccgtcgctga tcagaatgat ctgctgcgtt ccacccttgt gcttggagag cagcttctga
     1921 gccatcatca tggcatgatg catgttcgtg cccagaacgg actcgtccca gcgcacgtac
     1981 ggcagttgat cggccttgag ctctcgagca taggcggaga agccaatgat gtagaggctg
     2041 tcgcgcgtga actgggccgt gatgaggttg ttcaacgcca tcgcaacctt cttggcggcc
     2101 tggaagcttc cacgcagggc catcgaccac gataggtcga cgagcatcac ggtatgtgtc
     2161 gtggtgagtg attccgactt gtagacctcg aagtcttccg gctgcagccg tacaggcact
     2221 tccgggccct cgcgctgcat cgagttcatg atcgtctgtt tgaggtggag gtggaacgga
     2281 tcgccgaact cgtaggcctt cgtgtcgtcg gctcggtcac ccccgtagcc gcgctcgcgg
     2341 acggcgtgct tgccctgcgc gtccttcttg agctgggcgt agatctcgcc gagagccttc
     2401 tgaccgatct tccgggtgcc gcggggcgtt agctcccacg tgttgccctt gcgacggatg
     2461 tagcccgcct cctcgaggac ctcgaggagc tgcttcatct ggtcgagcgt ttccgcagcc
     2521 tcgccgccga gcagctctcg taccttgtcc gggtcgacct cgtcgatgtc gccgccgtac
     2581 tgcgtacgct ccaactgccg ctcgagttcg tccatgccct gcatgcgctc catgagctgc
     2641 agggcggcct ggaggtcgag ctcctcgtct ccccggaacg gatactggtt ccgcaggtct
     2701 cgcatcggat agaggaactc gaggtttgcg gcgagctcgg agaggcccgc ttgaagctgc
     2761 gggtcgccca ccttgtccat caacaggtcc tgcagctgct gccgcatatc tccgggcagc
     2821 gaatcgagca ggctctgcat ctgcgcggcc tggtgctgca tttgggcgat gagctcgtcg
     2881 agcgactgtg gggggttcgg accgaacatg tcgccgaact cgtccatgaa cgactggaag
     2941 tcggggtcgc cacccgccat gcgctcggag agcatgcgat tcagggcatc gaccatatcg
     3001 cgcatgcgct gcatgtcctc gggcgacatg ttggcgatct gctgggacag gtccttgaag
     3061 aacgtgtcca tcattgcttg cttgagctgg ttcatcagct cctggaactc gtgctgggct
     3121 tccgggtcga ggaactcgta gttctgcagc tccttgaccg cgcccccgag atcctgcggg
     3181 aggttttcga ggaagttgcg cttgcgctcg gcaatgctct tcagcatgtc ctggaactgc
     3241 tgcggatcgg cgttttggcc ctggccctgc gagcccgcgc cctgggagcc ggacatcgac
     3301 cccgcttggc ttccgctggc tccttgctgg ccgcctggcg atgattgctc cgccccctgc
     3361 tggccctcgg cgcccgcctc gccaccttcg tcgcctgaac cctgaggttg ctgcaggccc
     3421 gccttctcga gcgcttcgcg gatcgcctgt tgcatcgcat cgcggtcctg ttccagagga
     3481 ttgccctctg ggccatcgta atcagtgcga gggcgaccat ccggccccag ctcgccgcgc
     3541 tcagcctggc cgatgcgctc atccagggtg tggcgttcct tgtcgaggat gtcgtcgagc
     3601 ttcttgcgga tgtcgtcgat cgcagagcta aggttgaagc gatcgagggt ttgccggcgc
     3661 tgctgccgca gttgctgcag caggtccctc agcccttgca tgcgctgtcc catgggatcg
     3721 cgcatgccac gctgcagcag gttccgcaga gcgtgctgca ggtcgccgaa gttcatcagg
     3781 tcgtcggtga ggttctcgag gatggagtcc gggtcgagct caggtagctc ctggcttcca
     3841 tcccactcgg agtaacggaa tcggcttacc atatcagcct cgatactgcg ccttgccggc
     3901 gagacgctcc ttgttcaagc gcttgttgag gtgcaggcct tcgagcacga actccactgc
     3961 ggcagcggtt tgcggtgcgc tttccgcagg gccgaccttc cggattgctg catccatgcc
     4021 ctcgacctcg cgaaggatct tcacgtactg atccgatggg gtcgaatcgc ccacctcgat
     4081 ggagtagccg cctttgaact tcgcgactac cggctccagc tccgtgacgt tgtagtagcg
     4141 gttgaagacg gcgacgatgg cgccctggat cagcttctcg aggatctgct cctcccggcc
     4201 ttcgtcgacg gtttccaact ccaccttgcc cgagagggcg gggaggacgt agggcaggtc
     4261 ggagatgcgc ggcacgacct ccgcttcgtc gagccggatg gcgcggcgga gggcgttcgc
     4321 gaggaggctt tcgtagtcgg cgatggaggc gcgcaccgac acgccggacc gctgattcac
     4381 gtccgggctt cgccgcgcga gccgcgtgat ctcggctgcg atctccttca tgtactgggg
     4441 cacggtcacc gcatagtcgg ccgggagcgg cgcccgctcg ccctccatga tgcggatctc
     4501 gtgctcgacc gtccgcgggt agtgcgtgcg aatctgcgcg ccgtatcggt ccttgagcgg
     4561 cgtgatgatc cggcctcggt tcgtgtagtc ctcagggttc gcactcgcaa cgacgaagac
     4621 atcgagcggc aggcgaatgc ggtagccgcg gatctggacg tcgcgctcct ccatgatgtt
     4681 gagtagcccg acttggatgc gctcagccag gtccgggagc tcgttgatgg agaagatgcc
     4741 gcggttcgtg cggggaatca tgccgtagtg aatcgtgagc tcgtcggaaa ggtagcggcc
     4801 ttcggcgact ttgatcgggt ccacctcgcc gatgaggtcg gcgatcgaga tatccggggt
     4861 cgcaagcttc tccccgtagc gctcgtcgcg cccgatccag gcgatctcga cgttgtcccc
     4921 atggcgttcg acgctctccc ggcaggccgc gcagatgggg ttgaaggggt tatcgttgat
     4981 ctcgcagccg gcaatgaccg gaatctcatc gtcgagcagg tcgaccagcg agcgaatgat
     5041 gcgcgacttc gcctggcccc gctccccgag aaagatgatg tcctggccgg agaggatggc
     5101 gttctcaacc tgtgggatga cggtgtcctc gtaaccaatc acaccaggaa acagaggggc
     5161 cttcgagcgt atacgctgga tcaggttctt gcgcaactcc tctttgacgg ggagcacttt
     5221 gtaccccgaa tctcgcaact cgccgatcgt gctaggcttc ttcgtcacct tgcctgctac
     5281 ctccttggac cctccggccg ggtggccgcc gttgcgcccc ggctcgcgct tgccgcaccg
     5341 tccctaagac gatcgccggg atcggtggtt gccgggcccg gaaaagactg ggtgaagcgg
     5401 gggaactccc tagatggtgg tacggacatc caacctggga cgctgaattt gatcatactc
     5461 ccttcactag gggtgtcaag caagcctgaa tctgcacggc ggcccgcgtc gtggattctg
     5521 gaggcattcg ctggtatggt tgcggaatcc ggaggtggaa gggggagagg atatggctgc
     5581 gttcgacgta cgcgatctcg ctcctgagga cgttgagccg ctcggcgatg tcatctacga
     5641 ggccttccgc gacgttgcga cgaaacacgg attccgcccg acgttcgaca gtcccgctac
     5701 ggcgagcctc cttctcaaca ccttccgcgc cacggagctc gcgacgatcg tcacggctgc
     5761 tgagggcgcc gagccggtcg gcagcgcgag catgaacgtt cgcagcgaga cggcgggcat
     5821 cggtcccgtc gccgtgagtc cgcgggcgca ggcgggtggc gtgggccggg cgatgatgga
     5881 ggagctgctg cgccgggccg acgcggaggg ctgcacatcg gtccggttgg tgcaggccgg
     5941 ctacaacgtg acgtccttct cgctgtactc gaagctcggt ttcgtcgtcc gtgatgtcgt
     6001 gctgagtctc gaaggtcgca tcgaagcgcc ggagaccgac gcgtcggcgg tgcgagagat
     6061 gcgcccggaa gaaaccgcta cggccgccgc gctcgatacc cggctcaccg gcttccggcg
     6121 agagccggac tttgccctgc tgcgcggcat cggcccggga tgggtttggg agcaggacgg
     6181 catcgtgcgc ggctacctgc tctcgctcgg tgcgagtgga agcgtgcacc tggggc
//
LOCUS       DATMBB010000125         2671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_88134, whole genome shotgun sequence.
ACCESSION   DATMBB010000125 DATMBB010000000
VERSION     DATMBB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88134"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VNL92_02660"
     CDS             <1..866
                     /locus_tag="VNL92_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93641.1"
                     /translation="TATVPWLRTDPPAGVALGADVACAPGAACDRTATVEIVIDEGEL
                     ERGSNEAELQVWSLQQPGVMVSIPVSVSSTGPPPRPSPSPTATSIGTPSPNDTPSPTP
                     PAPSERTDVVAAELVAGCNPIGSAFPDGTPVAEVLASVEPDSVVTAVWRLPQPEGPWE
                     LFIPGQDAVSTLDTVNAHDGLFICTEGRTVVSQTIVIEGSGERTTMLQPGCNFVAAAR
                     ASTSAAFLGHVVPEDGVLALWWLDAREGHWLVYLPPAPPASSLDRVDRLAGVFVCMER
                     QGELRQLDLVP"
     gene            995..2107
                     /locus_tag="VNL92_02665"
     CDS             995..2107
                     /locus_tag="VNL92_02665"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HWO93642.1"
                     /translation="MMAHDPLHTKFCDMFGLEFPVIAFTHCKDVVVAVTNAGGLGVMG
                     EVGRTPEEIEQDVRWIRERVGEKAFGVDLLFPASIPPKANLEDLQAQIPEEHRKFVRQ
                     IKEKYNVPDPKREGARMRGGGMMSQEHARRQLDVVLEERVPLFASGLGNPAFIVEAAH
                     ERGIKVAGLIGKVRQARREIEAGVDFIIAQGYDAGGHTGEIGTFTLVPQVAAIAGDTP
                     VILAGGVGTGKHLAAALCLGAQGVWTGTIWLTARESDTDMIVKEKILAATEEDTLRSR
                     CMTGKPVRQLKTQWTEEWESPGAPSPLPMPLQGILTGDLMLAIGQYKIEPLMGSPAGQ
                     VVGTIHEMKPARDIVFDIIEEARDVLDGLAGEPVTA"
     gene            complement(2209..>2671)
                     /locus_tag="VNL92_02670"
     CDS             complement(2209..>2671)
                     /locus_tag="VNL92_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93643.1"
                     /translation="LVVSGGGLGRVRIRLDGKASTLRFGSGSYTVTDPEGTISLLVGG
                     GGLAEIEFILNGIASLIEVGDGGSITYTETTNEEGEVTGFEVKEVEGDVTLNDQPLAG
                     PVELVGPPMKFTDCSGRSWMTFNYPRTFRNAADCQYYVFNSLVSRALSRLR"
BASE COUNT          432 a          885 c          930 g          424 t
ORIGIN      
        1 cgacggcgac ggtgccttgg ctgcgcacgg atccgcccgc cggagtggcc ctgggagccg
       61 atgtcgcatg cgcgccgggc gcggcctgcg acaggacggc gacggtcgag atcgtcatcg
      121 acgaggggga gctcgagcgc ggctcgaacg aagcggagct gcaggtgtgg agcctgcagc
      181 agccgggcgt gatggtcagc attcccgtga gcgtcagctc caccggccct cctccgcgtc
      241 catcgccgag ccccaccgcc acatcgatcg gaacgccctc gccgaacgac acgccctccc
      301 ccacgccgcc cgctccctcg gagcgcaccg acgtcgtggc cgcggagctc gtggccggat
      361 gcaacccgat cgggagcgcg ttccccgatg gcactccggt cgccgaggtg ctcgcgagcg
      421 tcgaaccgga tagcgtggtg acggccgtct ggcggctgcc gcagccggaa gggccgtggg
      481 agctgttcat ccctggccag gatgccgttt cgaccctcga cacggtcaac gcgcacgatg
      541 ggctgttcat ctgcacggag ggccgtaccg tcgtcagcca aacgatcgtc atcgagggct
      601 cgggcgagcg aaccaccatg ctgcagcccg gctgcaactt cgttgcggca gcccgtgcca
      661 gtacgtccgc ggcgttcctg ggccacgtcg tgcccgagga cggcgtcctg gccctgtggt
      721 ggctcgatgc gcgggaaggc cactggctgg tgtacttgcc ccccgcgccg cccgcgagct
      781 cgctggaccg cgtcgatagg ctggccggcg tgttcgtctg catggagagg cagggagagt
      841 tgcgccagct cgatctcgtg ccttagccgg cggtccccgc tcgcccaatc ggtcgtttga
      901 cggaccaaca gtcgcgtctc tatacttcgt cacgcagtcg cccgagcgag gggcgtgcga
      961 ctgcgccccg ctatgtagtc tgcgaaagga cctgatgatg gctcacgatc cgctccacac
     1021 caagttctgc gatatgttcg gtctcgagtt cccggtcatc gcgtttaccc actgcaagga
     1081 cgtcgtcgtc gccgtgacga acgcgggcgg gctcggcgtc atgggggagg tcggcagaac
     1141 gccggaggag atcgagcagg acgtgcgctg gatccgcgaa cgggtgggcg agaaggcgtt
     1201 cggcgtcgac ctgctgttcc cggcgtcgat tcctccgaag gcgaacctgg aggacctgca
     1261 ggcccagatc ccggaggagc accgcaagtt cgtccggcag atcaaggaga agtacaacgt
     1321 gcccgacccg aagcgcgaag gagcgcgcat gcgaggcggc ggcatgatga gccaggagca
     1381 cgcccggcgg caactggatg tcgtgctcga ggagcgcgta ccgttgttcg cctccggtct
     1441 tgggaacccg gcgttcatcg tcgaggccgc gcacgaacgc ggcatcaagg tggcgggcct
     1501 catcggcaag gtgcggcaag cccgccgcga gattgaggct ggcgtcgatt tcatcatcgc
     1561 gcaggggtac gacgccggcg gccacaccgg cgagatcggc acgttcacgc tggtgccgca
     1621 ggtcgccgcg atcgcgggcg atacacctgt catcctcgcc ggcggcgtcg gcacgggcaa
     1681 gcatctcgcc gccgcgctct gtctcggggc gcagggggtg tggaccggga cgatctggct
     1741 caccgcacgc gaatcggaca ccgacatgat cgtgaaggag aagatccttg cggcgacgga
     1801 ggaggacacg ctccgttcac gctgcatgac gggcaagccg gtgcgccagc tgaagacgca
     1861 gtggacggag gagtgggagt cgcccggcgc gccctcgccg ctcccgatgc cgctgcaggg
     1921 catcctcaca ggcgacctca tgcttgcgat cggtcagtac aagattgagc cgctgatggg
     1981 ctcgcccgcg ggccaggtgg tggggacgat ccacgagatg aagccggcgc gcgacatcgt
     2041 cttcgacatc atcgaggagg cgcgcgacgt gctcgatggt ctggccggcg agcctgtgac
     2101 cgcttaggct tccttgcgca gcggatcgcc tcggtccggg cacgaacaga agaggggcga
     2161 ctcccgtttg ggagtcgccc ctctgcatgc cggcgcgggc ctccgttcct agcgcaggcg
     2221 ggagagagcc ctggagacga ggctgttgaa gacgtagtac tgacagtccg ccgcgttacg
     2281 gaaggtgcgc gggtagttga acgtcatcca cgaccgcccg gaacaatcgg tgaacttcat
     2341 gggcggcccg acgagctcga ccgggccggc gagcggttgg tcgttgagcg tgacgtctcc
     2401 ttcgacctcc ttcacctcga atccggtcac ctcaccctcc tcattcgtgg tctcggtgta
     2461 ggtgatactc cccccgtcgc caacttcgat caggctggcg atcccgttga gaatgaactc
     2521 gatctcggcg aggccgcctc cgcccacaag gagcgagatc gtcccctccg ggtcggtgac
     2581 ggtgtagctg ccgctgccaa atcggagcgt cgaggccttc ccgtccaagc ggatgcggac
     2641 cctgcccagg ccgcccccac tgacaacgag g
//
LOCUS       DATMBB010000126         4587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_366373, whole genome shotgun sequence.
ACCESSION   DATMBB010000126 DATMBB010000000
VERSION     DATMBB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366373"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VNL92_02675"
     CDS             complement(<1..601)
                     /locus_tag="VNL92_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93644.1"
                     /translation="MRETALVADVYERLLRAYGPQGWWPGGDDPFRVIVGAILTQNTA
                     WANVEKALQRLLEADALSIEGVRRLDEDALAELVRSSGYFRSKARKLKAFVRLVDERF
                     GGDVGALLALPQEELRQQLLECYGIGPETADAIVLYAAGKPAFVIDAYTTRIADRVGL
                     VPSHPTYDGYARLFAESLPAEASVFGEYHALLVEHGKKCC"
     gene            complement(598..1065)
                     /gene="tsaA"
                     /locus_tag="VNL92_02680"
     CDS             complement(598..1065)
                     /gene="tsaA"
                     /locus_tag="VNL92_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HWO93645.1"
                     /translation="MRLRRRDDPSLEAVTYRPIGHVRSAPRRAPAGGWRDVESSIVIE
                     DEFGAGLAGIEEYGHVWVVLALHGVAEEERRLLRRSPPGAPGEVGVFALRTQMRPNPI
                     GISAVRLLAVEGTTLRVRGLDAIDGTSVLDIKPYIPYYDSVPDASVPDWIGHP"
     gene            1131..3044
                     /gene="gyrB"
                     /locus_tag="VNL92_02685"
     CDS             1131..3044
                     /gene="gyrB"
                     /locus_tag="VNL92_02685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWO93646.1"
                     /translation="MTQASEYTARDIQVLEGLEAVRRRPGMYIGSTDSRGLHHLVYEI
                     VDNSIDEAMAGHCDRVEITIEADGAVLVADNGRGIPVDIHDKTGQSALETVMTVLHAG
                     GKFGGGGYKVSGGLHGVGASVVNALSSELWVEVRRKGDTNVYRQEFVRGMPRGPVFVA
                     GKCAANESGTTTWFRPDTDIFGEAQLDFETLAQRFREMAYLTRGVWVRFVDARQDVEQ
                     NFYFEGGTSSFVRHLNRGRAVLQPTPLWIEREVNGVSVEVSLQYNDAFSESMFSFANC
                     INTIDGGTHLTGFRTALTRVLNDYARKNRLLKDDDANLTGEDVREGLVAVVSVKLPEP
                     QFEGQTKTRLGNSEVRTIVESVVADGLTEYLENHPQDGRRIIDKCLTAARAREAARKA
                     RDLVVRKSALDGAMLPGKLADCQERDPERAEIYLVEGDSAGGSAKQGRDRRYQAILPL
                     WGKILNVEKARLDKIFTHDAIRAIITALGTGVDETFDITKLRYHYVIIMTDADVDGSH
                     IRTLLLTFFYRHMPELIQAGNLYIAQPPLYRIQNGRQHEWMFSDREKDAYLAQLKGKN
                     VHVQRYKGLGEMNPDQLWETTMDPERRVLLKVTAENAVLADETFRDLMGEDVEARKRF
                     IQSHASQVRNLDV"
     gene            3051..3470
                     /locus_tag="VNL92_02690"
     CDS             3051..3470
                     /locus_tag="VNL92_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93647.1"
                     /translation="MARLILATRPDVLPLSFSGESSDLVWFLSFAAVEQYGSQHELSK
                     AATVLRHKHKVRLRPLLRFTAHDDDDAEELERAWQDPAPLADVARRIAGLLEGDDREL
                     AAYTKDFPGLADQLRELASLADVAAERGAQIRMTYEL"
     gene            3506..4195
                     /locus_tag="VNL92_02695"
     CDS             3506..4195
                     /locus_tag="VNL92_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO93648.1"
                     /translation="MLWSERREARAYNRWMAAVDPLAQHMRWNMGPTGMYLVNTPVLG
                     LAEELGLEAHHSILDIGCGRGSLARYLSRVVRFEAPPVAMDVSTVMLRLALQDADEEA
                     PVSLVAGSASSLPFEDNTFDFVIVAHVWKHLDDRVFLHNLFEIERVLRPGGSCVAWEF
                     APRSCVWLNRANRAILTLGEVKSAHLRGFHVIASAAIDARFAHIRRLQLGPFFWPPIP
                     RVSVRLYKSEA"
     gene            complement(4177..>4587)
                     /locus_tag="VNL92_02700"
     CDS             complement(4177..>4587)
                     /locus_tag="VNL92_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HWO93649.1"
                     /translation="RFDVSDIEVRRRGPSYTADTLAALAEGGDELFLILGADAVADLP
                     NWVRPEEIVRQAWIAVAPREGMGEGAMENALRLVAGLAERLLTSPMLTVEISATELRE
                     RARNGESVRYFVPDAVDRYLRQKGLYRADHASLL"
BASE COUNT          861 a         1566 c         1363 g          797 t
ORIGIN      
        1 tacagcactt ctttccgtgt tcaacgagca gggcgtggta ctcgccgaac accgacgcct
       61 ccgccggcag agattccgca aagagccgtg catagccatc atacgtcgga tgcgacggga
      121 cgagcccaac gcgatcggcg atgcgcgtgg tgtaagcatc gataacgaac gcgggcttgc
      181 ccgccgcgta caggacaatc gcatcggccg tttccggacc gatgccatag cattccaaga
      241 gctgctggcg caactcctcc tgcggcaagg cgaggagcgc gcctacgtcg ccaccgaacc
      301 gctcgtcgac cagcctcacg aatgctttca gcttgcgcgc cttcgaccga aaataccccg
      361 acgagcggac aagctcggct aaggcgtcct catcgagcct ccgcacccct tcgatgctca
      421 gcgcatcggc ctcgagcagt cgctgcaggg ccttctccac gttggcccac gccgtgttct
      481 gcgtcaggat ggcgccgacg atgactcgga acggatcgtc gccgcctggc caccagccct
      541 gcgggccgta cgctcgcagg agacgctcgt acacatcggc cacgagggcc gtctccctca
      601 tgggtggcct atccagtcgg gaacgctcgc gtccgggacg ctatcgtagt acggaatgta
      661 gggcttgata tcgaggacgc tggtcccatc gatggcatcg agtccccgaa cacggagggt
      721 cgttccctcc accgcaagca ggcgcaccgc ggatatgccg attgggtttg gccgcatctg
      781 cgtcctcagc gcaaagacgc ccacctcgcc gggcgcgccg ggagggcttc gccgcaagag
      841 gcgccgctcc tcctccgcta cgccgtggag agctaacacc acccacacgt gaccatactc
      901 ctcgatgccg gcgagaccag ccccgaattc gtcttcgatc acgatgctgg actcgacgtc
      961 gcgccagcca ccagccggcg cgcggcgcgg cgcagaccgc acgtgcccaa taggacggta
     1021 tgtgacggcc tcgagcgaag gatcgtcgcg cctgcgcaat ctcacggtcc gcctctcggc
     1081 atcgtgtcca gcttccgttc gaacgttacc ctgtgataaa atttatacat atgacacaag
     1141 catctgaata taccgcacgt gacatccagg ttctcgaagg gctagaggcg gtccgccgcc
     1201 ggcccggaat gtacatcggc agcaccgact cccgcggtct ccaccacctc gtctacgaga
     1261 tcgtcgacaa ctcgatcgac gaggcgatgg ccggccactg cgatcgggtc gagatcacca
     1321 tcgaggcgga tggtgccgtc ctcgtcgctg acaacggccg gggcattccc gtcgatattc
     1381 atgataagac aggccaatcc gcactcgaga ccgtcatgac cgtgctccac gccggcggca
     1441 agttcggcgg cggcgggtac aaggtctcgg gcggcctcca cggcgtaggc gcctcggtcg
     1501 tcaacgccct ttcgtctgag ctctgggtgg aagtgcgccg caagggcgat acgaacgtgt
     1561 accggcagga gttcgtccgc ggcatgcctc gtggccccgt gttcgtcgcg ggcaagtgtg
     1621 ctgcaaacga gtccggaacg acgacgtggt tccgcccgga caccgatatc ttcggggagg
     1681 cgcagctcga cttcgagacg ttggcgcaac gcttccgaga gatggcctac ctcactcgag
     1741 gcgtctgggt tcgattcgtc gatgctcggc aggacgtcga gcagaacttc tacttcgaag
     1801 gcggcacttc gtcgttcgtg cgccacctga accgtgggcg cgccgtcctc cagccaacgc
     1861 ccctgtggat tgagcgcgaa gtcaacggcg tctcagtgga agtttcgctg cagtacaacg
     1921 acgccttcag cgagtcgatg ttcagcttcg caaactgcat caacaccatc gacggcggca
     1981 cgcacctcac cggcttccgt acggccctga cgcgcgtgct caacgactac gcccgtaaga
     2041 accgcctcct gaaggatgac gacgcaaacc tcaccggcga ggacgtgcgg gaaggtctcg
     2101 tcgccgttgt aagtgtaaag ctgcccgaac cgcagttcga gggccagaca aagacgcgtt
     2161 tgggcaacag cgaggtccgc acgatcgtgg aatcggtcgt ggccgacggc ctgaccgagt
     2221 acttggagaa ccacccgcag gacggccgcc gcatcatcga caagtgcctc accgccgcac
     2281 gcgcccgtga ggccgcgcgc aaggcgcgcg acctggtggt tcgcaagagc gccctggacg
     2341 gcgcaatgct gcccggtaag ctcgccgact gtcaggaacg cgatccggaa cgcgcagaga
     2401 tctacctagt ggaaggcgat tcggccggcg gctcggcgaa gcaggggcgc gatcggcgat
     2461 atcaggccat tctgccgctg tggggcaaga ttctgaatgt cgagaaggct cgcctcgaca
     2521 agatcttcac gcacgatgct atccgcgcca tcatcaccgc ccttggcacg ggcgtcgacg
     2581 aaacgttcga tatcacgaag ctgcgctatc actacgtcat catcatgacc gatgctgatg
     2641 tggacggatc gcacattcgc acgctcctcc tcacgttctt ctaccgtcat atgcccgagc
     2701 ttatccaggc cggcaacctc tacatcgctc aaccgccgct gtaccgcatc cagaacggcc
     2761 gccagcacga atggatgttc agcgaccgag agaaggacgc ctacctcgcg cagctcaagg
     2821 gcaagaatgt ccacgtgcag cgctacaagg gcctcggcga gatgaacccg gaccagctgt
     2881 gggaaacgac gatggacccc gagcgccgtg tcctgctcaa ggtgaccgcg gagaacgccg
     2941 tgctcgccga cgagacgttc cgcgatctca tgggcgagga tgtcgaggca cgcaagcgct
     3001 tcatccagag tcacgcatcg caagtccgca acctcgacgt ctaagccgcc gtggcccggc
     3061 tcattctcgc gacacgcccc gatgtgctac cgttgtcgtt ctcaggggag agtagcgacc
     3121 tcgtatggtt cctttctttc gccgccgtcg agcaatatgg ctcccagcac gagctctcga
     3181 aggcagcgac cgtcctgcgc cataagcaca aggttcgcct ccggccactc ctccggttta
     3241 cggcccatga cgacgatgat gcggaagagt tggagcgcgc gtggcaggac cccgcgcccc
     3301 ttgccgacgt tgcccgccgt atcgccgggc tcctcgaagg cgatgaccgt gagctcgcgg
     3361 cctacacgaa ggacttccca gggctcgccg accagctccg cgaactcgca tccctggccg
     3421 acgtcgcggc cgaacgtggc gctcagatcc gaatgaccta cgagctgtga cgagacgggg
     3481 ctctattccg cgctaaactg cttccatgct gtggtccgaa cgtcgcgagg cacgagccta
     3541 caaccgctgg atggccgccg tcgatccctt ggcccaacac atgcgctgga acatggggcc
     3601 aaccggcatg tacctcgtaa acacgccggt actgggactc gcggaagagt tgggcctcga
     3661 agcgcatcac agcatcctcg acattggctg cggacgtggc tccctcgccc ggtatctatc
     3721 ccgagtcgtt cggttcgaag cgcctccggt cgccatggac gtatcgaccg ttatgcttcg
     3781 cttggcattg caggacgctg acgaggaggc acccgtctcc ctcgtagcgg gatccgccag
     3841 ctcactcccc ttcgaggaca acactttcga cttcgtgatc gtcgcccatg tgtggaaaca
     3901 cctcgacgac cgtgtgttcc ttcacaacct gttcgagatc gagcgcgttc tgcggccggg
     3961 aggatcgtgt gtggcgtggg aattcgctcc gcgcagctgt gtctggctca acagggccaa
     4021 ccgcgccatt ctcacgctcg gcgaggtgaa aagcgcacac ctccggggct tccacgtcat
     4081 cgcctcggcc gccatcgatg cgcgcttcgc ccacatccgg cgccttcagc tcggcccgtt
     4141 cttctggcca cctatcccgc gcgtatccgt ccgcctctac aagagcgagg cgtgatccgc
     4201 acgatacaag cccttctgac gaaggtagcg atccacggcg tccggaacga agtagcgcac
     4261 gctctcgccg ttccgggctc gctcccggag ctctgtggcg ctgatctcga ccgtcagcat
     4321 cggactcgtc agcagccgtt cagccaagcc cgcgaccagc cggagcgcat tctccatggc
     4381 accctctccc atgccctccc ggggtgcaac tgcgatccac gcctgcctca cgatctcctc
     4441 gggccgaacc caattcggca gatcggccac ggcatcggcg ccaagaatga ggaacagctc
     4501 gtcccctccc tctgccaggg ccgccagcgt atcggcggtg tacgaggggc cgcgacgtcg
     4561 aacctcaatg tcgcttacgt cgaaccg
//
LOCUS       DATMBB010000127         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_365812, whole genome shotgun sequence.
ACCESSION   DATMBB010000127 DATMBB010000000
VERSION     DATMBB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365812"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VNL92_02705"
     CDS             complement(<1..271)
                     /locus_tag="VNL92_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO93650.1"
                     /translation="MASALPQWGLNRLDFRSVASFAEDAARIERLGWDWAFIPDSQLL
                     LPDPYVMLAGAAQATRRLGLGVLMTNPVTRDATVTASSIATVDALA"
     gene            complement(271..663)
                     /locus_tag="VNL92_02710"
     CDS             complement(271..663)
                     /locus_tag="VNL92_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWO93651.1"
                     /translation="MSLSENVRKLAEGKTYCIVATTMRDGSPQTSLVWVDTDGEHVIF
                     NTDERRLKAKNLRRDPRVAITLVSPENAYDMAMIRGRAVEFVRDGADAHLDKLAKKYL
                     GADSYPFRQPDDERVMIKVAVDRVATMQ"
     gene            complement(753..2138)
                     /locus_tag="VNL92_02715"
     CDS             complement(753..2138)
                     /locus_tag="VNL92_02715"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWO93652.1"
                     /translation="MSWKERYAAKLISADDAARFVKSGDRVRSAMCSPFQTPIAFVTA
                     LARRAAELRDVDIDVTWSAASELGLLAPGTEDAWSTYSAFAYGRTEEERLTQRSPQTN
                     FVPLHPSFIGRLGRGNHRDDFRQRWRDADVYAVMVTPPDAAGNVTFGFNLWNARVQMD
                     GARIIIGEVNERLPIVPGGDNWAPADAFDYFIETIDMPMPSTSLDETPEEEREASEVC
                     AALMAELIDNGDTVMFGGGAMPIRFQPFLEHKEDLGCHSEVVVPLDLMRKGVITNKKR
                     NLAPGQVSCTAISAWYGQADLDYIHLNPRFDMRDMAVNNDPRYICQNDRLVAVNAPLE
                     ITLWGEINVERVGPRYLRGVGGQVEFVMGALLSEGGRSIHGVVSRKKNANGEWVSAIV
                     PEFTHPGVASIPRHLADFVVTEHGVARLMGKTERERANELIAIAHPDFRPELREAAKR
                     FFGLGKTIHFT"
     gene            2378..>3572
                     /locus_tag="VNL92_02720"
     CDS             2378..>3572
                     /locus_tag="VNL92_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWO93653.1"
                     /translation="MTTAGTTTLLFTDLVGSTELLDRLGDEAAERLRREHFRLLRESV
                     RTAGGHEVKNLGDGLMVAFGSAGDAVRCAVLMQQSVERRNREIGGEELSVRIGLHVGE
                     PIRDEDDYFGTPVVIAKRLCDRAEGGQVLASTLVRDLIGARPEFGFRELGPLALKGIA
                     SPLAACEVTWTPAAPGEAPAIPLPPQLSSVERTPFVSREGELRQLRAQWELVQQGRIQ
                     LALIAGEPGIGKTRLATELALEAHGDGATVLFGWCDEDSVAPYRPWVEAIRYYARTCP
                     ADTFLEQAGAGGAELARIVPELPASLSLPPATEAAGEDARYRLFEAVLTLLVAASTSR
                     PLVIILDDLQWADGPTLQLLKHIARSPRHAALFLVGTYRDTDLGRAHPLADALADLRR
                     ARAFTR"
BASE COUNT          563 a         1213 c         1203 g          593 t
ORIGIN      
        1 gcgcgagggc gtcgacggtc gcaatcgacg aggccgtgac cgtcgcgtca cgtgtcaccg
       61 gattcgtcat gagcacgccg aggccaaggc ggcgtgtcgc ctgcgcggcg cccgcgagca
      121 tcacgtaggg atcgggcagg agaagctgcg aatcggggat gaaggcccag tcccagccga
      181 ggcgctcgat gcgcgcggcg tcctcggcga acgaagctac gctgcggaaa tcgagccggt
      241 tgagtcccca ctggggcaag gcgctggcca ctactgcatg gtggcgacgc ggtcgacggc
      301 gactttgatc atgacgcgtt cgtcatcggg ctgccggaac gggtacgagt cggcgccgag
      361 gtacttcttg gccagcttgt cgaggtgtgc atcagcccca tcccgcacga actcgacagc
      421 ccgtccgcgg atcatcgcca tgtcgtaggc attctcgggg cttacgagcg tgatggccac
      481 gcgcggatcg cggcgaaggt tcttcgcctt cagccggcgc tcgtcggtgt tgaagatgac
      541 atgctcgccg tcagtgtcga cccaaaccag gctcgtctga ggcgagccgt cgcgcatcgt
      601 cgtggccacg atgcaatagg tcttaccctc ggcgagcttg cggacgttct ctgagagcga
      661 catggggcct cctacgcttg cttttctacc gcgccattct gactcacgag ttgccgcatg
      721 tcgagccccc ggatgggcga ggcacgcctg acctaggtga agtgaatcgt cttgcccagg
      781 ccgaagaagc gcttcgccgc ttcgcggagc tcgggccgga agtcgggatg cgctatggcg
      841 atgagctcgt tcgcccgctc gcgctccgtc ttgcccatca gtcgagcgac gccgtgctcg
      901 gtgaccacga aatcggccaa gtgacgcggg atcgacgcca cgccgggatg agtgaactcc
      961 ggcacgatgg ccgagaccca ctcgccgttc gcgttcttct tgcgagagac aacgccgtgg
     1021 atcgagcgtc ccccctccga gagaagggcg cccatgacga actccacttg gccgccgacg
     1081 ccgcgcaggt agcgcggccc cacccgctcg acattgatct cgccccataa cgtgatctcg
     1141 agcggcgcgt tgacggcgac caggcggtcg ttctggcaga tgtagcgcgg atcgttgttg
     1201 accgccatgt ctcgcatgtc gaagcggggg ttgaggtgga tgtaatcgag gtcggcctgg
     1261 ccataccatg ccgagatggc cgtgcaggac acctgtcccg gcgcgaggtt gcgcttcttg
     1321 ttggtgatga cgcccttgcg catgaggtcc agcggaacca cgacctcgct gtggcagccg
     1381 aggtcctcct tgtgctcgag gaagggctga aagcggatcg gcatcgcgcc gccgccgaac
     1441 atgaccgtgt cgccgttgtc gatgagctcg gccatgagcg ccgcacacac ctccgaggcc
     1501 tcgcgctcct cctcgggcgt ctcgtcgagg ctggtggagg gcatgggcat gtcgatggtt
     1561 tcaatgaagt agtcgaaggc gtcggcgggc gcccaattgt cgccgcccgg cacgatgggc
     1621 aggcgctcgt tcacctcgcc gatgatgatg cgtgcgccgt ccatctgcac gcgcgcgttc
     1681 cacaggttga acccgaaggt tacgttcccg gccgcgtccg gcggcgtcac catcaccgcg
     1741 tacacgtcgg cgtcgcgcca ccgctgtcgg aagtcgtcgc ggtggttgcc gcgaccgagc
     1801 cggccgatga acgatgggtg caagggaacg aagttcgtct gcgggctgcg ttgcgtcaga
     1861 cgctcttcct ccgtccgccc ataggcgaag gcggagtacg tgctccacgc atcctccgtg
     1921 ccgggcgcga gcaacccgag ctccgaggcg gccgaccacg tgacatcgat gtcgacgtcg
     1981 cgcaactccg cggcgcgccg cgcgagcgcc gtgacgaagg cgatgggggt ttggaacggg
     2041 gaacacatag cgctccggac gcgatcgccg ctcttgacga atcgagctgc gtcgtcggcc
     2101 gagatgagct tggctgcgta gcgctctttc cacgacatcg cgcatcctcc ctcgggcgat
     2161 gaatcggcgc gaggttccca cgtacaccgt gccctcgtca agcgatgccg gaggcgagca
     2221 tgccgccgaa cggggacgca tgcagccaac cggcggtgga gcttgcccgc cgcccatttc
     2281 cactcatggc ggcgctatga gcccggaggc ggcagcacac gaaggccgtg gtatcatgcc
     2341 cgcgctaagc gaaccgtaag gcgagcgggg gtggtatatg accaccgcgg gcacaaccac
     2401 actcctgttc accgatctcg tcggctcgac cgagctcctc gaccgcctcg gcgacgaggc
     2461 cgccgagcgc ctccgccgcg agcactttcg cctgctccgc gagtccgtcc gcacggccgg
     2521 cggccacgag gtcaagaacc tcggcgatgg gctgatggtc gccttcggca gcgccggtga
     2581 cgccgttcgc tgcgctgtcc tgatgcagca gagcgttgag cggcgcaacc gcgaaatcgg
     2641 aggcgaggag ctttcggtgc gcatcgggct gcacgtcggc gagcccatcc gcgacgagga
     2701 cgactacttc gggacgcccg tcgtgatcgc taagcgcctc tgcgaccgag ccgagggcgg
     2761 acaggtgctc gcttcgaccc tcgtgcgcga cctcatcgga gcgcggccgg agttcggctt
     2821 ccgcgagctc ggtccccttg cactgaaggg catcgcctcc cccctcgctg catgcgaggt
     2881 gacctggacg cccgcggcgc ccggcgaggc cccagcgatt ccgctgccgc cacagctgag
     2941 cagcgtggag cgcacgccgt tcgtcagccg cgagggcgag ctacgccagc tccgagccca
     3001 gtgggagctt gtgcaacaag ggcgcatcca gctcgccctt atcgctggcg agccgggcat
     3061 cggcaagaca cgcctcgcca ccgagctcgc gctcgaggcg cacggcgatg gcgccacggt
     3121 gctgttcggc tggtgcgatg aggattccgt cgcgccgtat cggccttggg tcgaggcgat
     3181 acgctactac gcgcggacgt gtcctgccga cacgttcctc gaacaggcgg gggctggcgg
     3241 agccgagctc gctcgaatcg tcccagagct cccggcctcg ctcagcttgc ctcccgcaac
     3301 cgaggcggcc ggcgaggacg cgcgctaccg gctgttcgag gccgtgctga ccttgctcgt
     3361 ggcggcatcc acatcgaggc cgctcgtcat catcctcgac gacctccaat gggccgatgg
     3421 ccccacgctc cagctgctga aacacatcgc ccgctcgccg cggcacgccg ccctcttcct
     3481 ggtcggcacg taccgcgata cggacctcgg gcgcgcccat ccgctggcgg atgcccttgc
     3541 cgatctacgg cgggcgcgtg ccttcacgcg aa
//
LOCUS       DATMBB010000128         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_306967, whole genome shotgun sequence.
ACCESSION   DATMBB010000128 DATMBB010000000
VERSION     DATMBB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306967"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="guaA"
                     /locus_tag="VNL92_02725"
     CDS             <1..805
                     /gene="guaA"
                     /locus_tag="VNL92_02725"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWO93654.1"
                     /translation="FVDHGLMRANEAEQVVSLFRNAYNIPLVHVDAETLFLSGLRGVT
                     DPEAKRKFIGRTFIDVFEAEAAKIGGADFLAQGTLYPDVIESVSFTGGPSVTIKSHHN
                     VGGLPERMKLKLLEPLRELFKDEVRALGRELGLPESMIRRHPFPGPGLAIRVPGEVTR
                     ERLDILRKADAIYLDEIRNAGLYDAIWQAFVVLLPVRTVGVMGDERSYDYACALRAVT
                     STDGMTADYYPFDHGFLGRVATRIINEVRGINRVTYDITSKPPGTIEWE"
     gene            complement(760..1500)
                     /locus_tag="VNL92_02730"
     CDS             complement(760..1500)
                     /locus_tag="VNL92_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93655.1"
                     /translation="MSKMPRNVVNRWTHKHSPERSMLALTENGRLAVRRANPWVGLWI
                     VLSAAFMGIMDVFITVVAAPAIRSDLGATVADTQLVLAAYNLAFALVLITSGRLGDLF
                     GRRRIFSPGLALFTAGSLAASLSPDPITLILARALLGVAAGLMMPQVFSIIQANFEGE
                     DRGKAFGAFAFVSGLAATTAQVVGGVLITADLFALGCRAIFLVNVPIGVLALAAAQVW
                     VGAFPFASAALDIPHSHSIVPGGFEVMS"
     gene            complement(1457..1972)
                     /locus_tag="VNL92_02735"
     CDS             complement(1457..1972)
                     /locus_tag="VNL92_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWO93656.1"
                     /translation="MKPRIDYRRVAPEALEAVLTLDTYVRQCGLEPSLLDLVNIRASQ
                     INGCAYCLHLHTREARSRGESEERLHLLNAWRESPLYSDRERAALAWTEAVTLVSGAH
                     VPDEVFAQARRHFSEEELVKLTVAIATINALNRLAISMRALHPAKTGAASPPAGAHVE
                     DAPQRREPLDA"
     gene            2193..2456
                     /locus_tag="VNL92_02740"
                     /pseudo
     CDS             2193..2456
                     /locus_tag="VNL92_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527232.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
BASE COUNT          427 a          808 c          818 g          403 t
ORIGIN      
        1 gttcgtggat catggcctga tgcgcgccaa cgaggccgag caggtcgtca gcctgttccg
       61 caacgcctac aacatcccgc tggtgcacgt ggacgccgaa accctgttcc tctccggcct
      121 caggggcgtc accgacccgg aggccaagcg caagttcatc ggcaggacct tcatcgacgt
      181 gttcgaggcc gaggcggcga agatcggcgg cgccgacttc ctcgcccaag gcacgctcta
      241 cccggacgtg atcgagagcg tgtcatttac cggcggtccg tccgtcacca tcaagtcgca
      301 ccacaatgtc ggcggcttgc cggagcgcat gaagctgaag ttgctggagc cgttgcgcga
      361 gctgttcaag gacgaggtgc gcgcgctggg tcgcgagctc ggcctcccgg aatcgatgat
      421 ccgccgtcac ccgttccccg gtccgggcct cgccatccgc gtccccggcg aggtcacgcg
      481 cgagcggctc gacatattgc gcaaggccga cgccatctat ctcgacgaga tccgcaatgc
      541 cggcctctac gacgcgatct ggcaggcctt cgtcgtgctg ctgccggtcc gcaccgtcgg
      601 cgtgatgggc gacgagcgct cctacgacta tgcctgcgcg ctgcgcgccg tcacctcgac
      661 cgacggcatg acggccgact actatccttt cgaccacggc ttcctcggcc gcgtcgccac
      721 ccgcatcatc aacgaggtgc gcggcatcaa ccgggtgacc tacgacatca cctcgaagcc
      781 gccggggacg atcgagtggg agtgagggat atccaaggcc gcgctggcga aggggaaggc
      841 cccgacccag acctgcgctg ccgcaagcgc aagtacaccg atggggacat tgaccaggaa
      901 gatggccctg caccccagag cgaagagatc ggcggtgatg aggacgccgc ccacgacctg
      961 cgcggtcgtc gcggcaaggc ctgacacgaa cgcgaacgct ccgaaggcct tgccgcgatc
     1021 ctctccttcg aagttggcct gaatgatgga gaacacctgc ggcatcatca gccccgcagc
     1081 tactcccagg agagcgcggg cgaggatgag cgtgatcgga tcgggcgaga ggctcgccgc
     1141 cagcgagccc gccgtgaaca gcgcgaggcc cggggagaaa atccgccggc ggccgaagag
     1201 gtcgccgaga cgcccgctgg tgatcaggac aagggcgaag gcgaggttgt aggcggcaag
     1261 aaccagctgt gtgtcggcca cggtcgcgcc gagatcggag cggatggcag gcgcggcgac
     1321 gacagtgatg aagacgtcca tgatgcccat gaatgcggcc gaaaggacga tccagagccc
     1381 cacccatgga ttggcgcggc gcaccgccag ccgaccattc tccgttaatg caagcatgct
     1441 tcgctccggg ctgtgcttat gcgtccagcg gttcacgacg ttgcggggca tcttcgacat
     1501 gcgcgcccgc cggcggactg gccgcgccgg tcttcgccgg gtggagcgcc cgcatgctga
     1561 tcgcgagccg gttcaaggcg ttgatggtgg cgatcgcgac ggtcagcttg acaagctctt
     1621 cctcggagaa gtggcggcga gcctgggcga acacctcatc cgggacgtgc gcccccgaaa
     1681 cgagggtgac ggcctccgtc caggcgagag cggcgcgttc ccggtcgctg taaagtggcg
     1741 actcccgcca ggcgttgagg agatggaggc gttcctcact ctccccgcgg ctgcgcgcct
     1801 cgcgcgtgtg caaatggagg caataggcgc acccgttgat ctgcgaggcc cggatgttga
     1861 cgagatcgag gaggctcggc tcgagcccgc actgtcgaac ataggtatcg agcgtcagca
     1921 cggcctcgag cgcttcggga gccactcggc gatagtcgat tctcggcttc atgcgtcctt
     1981 cccgtgttgc catttgatga ccgcctgcaa agacggggca gacgcagcgc tgtgacagat
     2041 gcctgcgatt ggcgccggac gacctcggtg gacgcgtgct tccgggccac gcgctcggat
     2101 acggttgctc gcggacctgt cacgccggcc gatctggacc gtctatccat ctcggagttg
     2161 gacttccaat ctcggagttg gacttcactg cgatgccgac ggtcagccat gaccttgagg
     2221 ccctgaggcc acgcctgttc ggccttgcct atcgcatgct cggctcccgc gaggaggcgg
     2281 aggacgccgt gcaggaagcg ttcttgcgct ggcatcgggc cgacacggcc gaggttcgct
     2341 cgccggaggc atggctcgtc accacactat cgcgaggccg ttcggcgcca atttcgtgct
     2401 cgattttcca atcgaagaac ggctcgccgt cgcgctcctg ccgcagatcg tcgatg
//
LOCUS       DATMBB010000129         5289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_45000, whole genome shotgun sequence.
ACCESSION   DATMBB010000129 DATMBB010000000
VERSION     DATMBB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45000"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VNL92_02745"
     CDS             complement(<1..304)
                     /locus_tag="VNL92_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWO93657.1"
                     /translation="MASEPAPLVQAKSIYDAAEPSDGYRVLATRYWPRGVSRGRCDEY
                     TAALAPSRKLLHDFRAGEVDWDAYRERYLAELAGSEDAQERLGALRTKARDTTVTVM"
     gene            complement(325..1623)
                     /gene="purB"
                     /locus_tag="VNL92_02750"
     CDS             complement(325..1623)
                     /gene="purB"
                     /locus_tag="VNL92_02750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWO93658.1"
                     /translation="MIPRYSRPEMSAVWAEENKYRQWLRVEIAAARAWADVTGIIPRE
                     DLACIEANARFDLDEVRRYEREQHHDVTAFLRSVSDSLGPEGRWVHYGLTSSDVVDTA
                     LALQLVQASDLLLRELDALTVAVAEKAREHKHTLQMGRTHGVHAEPTTFGLKLAAWVV
                     ELQRGRARLEQAREQIAVGKLSGPVGTHASVPAAVEERALAELGLAVEPVSSQIVHRD
                     RHAHFVATLALIGSSLEKFATEIRGLQRTEVREVEEPFSPGQKGSSSMPHKRNPEKVE
                     RICGLARLLRGYALTSLENVALWHERDISHSSAERIIFPDACLVLDYALDLMTFVMRG
                     LHVYPERMRRNLELSYGLPFSQRVLLALVDAGLSRDEAYELVQRNAMRAWSEERQFLD
                     LLCEDAEVTRAIAKEGLTKLFDPSYYVREVDEAFRRAGLE"
     gene            complement(1626..2897)
                     /gene="purD"
                     /locus_tag="VNL92_02755"
     CDS             complement(1626..2897)
                     /gene="purD"
                     /locus_tag="VNL92_02755"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWO93659.1"
                     /translation="MRVLIVGSGGREHALAWKIGQSPKLDALFVAPGNAGIARLAAAV
                     PSVRSDDEIIALARGERIDLVVVGPEAPLATGLVDRLLAAGIAAFGPTAGAARIESSK
                     AFAKQLMFQHGIPTAAARIFDDPDEALAYVRTLEAPPVVKADGIASGKGVTVPATREE
                     AEQAVVRAMLDREYGAAGARVLVEERLEGREASAHALSDGRHVLQLPFARDYKRALDG
                     DRGPNTGGMGAYSPLEDVTPELEETVQRTVTEPTIAALAAEGAPFRGLLYPGLMLTPD
                     GPKVIEFNARFGDPETQVLLPRIESDLLDLLWACATGDLGEARLRVSPKAAVGVVMAS
                     GGYPGPYRTGVPIEGLESVDDDVLVFHAGTALNDSGQLVTSGGRVLTVVALGDTVAQA
                     TARAYDNVGRIRFEGAHVRRDIAAPAGFGEL"
     gene            complement(2910..3092)
                     /locus_tag="VNL92_02760"
     CDS             complement(2910..3092)
                     /locus_tag="VNL92_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93660.1"
                     /translation="MSIAVLVALYVAGMLVAAWAAQRRGYWWPLGAGLGIAVGPAAPV
                     VMFLLPDRRRRRRRGV"
     gene            complement(3094..4761)
                     /gene="guaA"
                     /locus_tag="VNL92_02765"
     CDS             complement(3094..4761)
                     /gene="guaA"
                     /locus_tag="VNL92_02765"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWO93661.1"
                     /translation="MTNEASKGPRLRTTDDLEVSAYLELASGAPGEAAEAAPAPAPPK
                     EPEAIVVLDFGSQYSMLIARRVRELNVYCELVPHDAPWERIARLRPKGFILSGGPASV
                     YEPDAPLAPPYVYEARVPVLGICYGMQLLAHQMGGRVAPSDHREYGHAVIHRGASDIG
                     LFEGLPASLPVWMSHGDRITEMPPGFAALAHSENSPIAAMGNEQGMVGIQFHPEVVHT
                     PQGREMLRNFLFRLCGCSGTWTPGNFISEAVDAIREQADGRIICALSGGVDSAVTAAL
                     VHRAVGDRLTCIFVDNGLLRRGEPERVVETFRRSMGINLVHAKAEDRFLACLAEVTNP
                     ETKRVRIGEEFIRVFESEAGKLGQVDFIAQGTLYPDVIESASSGTAAAAKIKTHHNVG
                     GLPKDMKFRLVEPLRNLFKDEVRKVGVELGLPDDIVYRQPFPGPGLAIRILGEVTREK
                     LEVLRSADAVVMDEIRRAGLYYELWQSFAVLTDSRTVGVMGDFRTYGHVVAIRAVTSE
                     DAMTADWARLPYDLMARISSRIVNEVPGVNRVVYDITSKPPGTIEWE"
     gene            4897..>5289
                     /locus_tag="VNL92_02770"
     CDS             4897..>5289
                     /locus_tag="VNL92_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HWO93662.1"
                     /translation="MQIVAEDDAASIDLAVQALRRGHLVAFPTDTVYGVAALAADEQA
                     CSALFSAKGRPPERAIPIFIPSIDLLGEVASGFGEAELRLARAGLPGPLTIVLRRAPA
                     FRSAALVGDDTVGVRIPNHRWVLALLRTL"
BASE COUNT          861 a         1865 c         1734 g          829 t
ORIGIN      
        1 acatcacggt cacggtcgta tcgcgagcct tcgtacggag ggccccgagg cgctcctgcg
       61 cgtcctccga gcctgcgagc tcagccaggt accgctcgcg gtaggcgtcc caatcgacct
      121 cgccggcgcg gaagtcgtgc aggagcttgc gtgagggtgc gagcgctgcg gtgtactcat
      181 cgcagcgccc cctgctcacg ccgcggggcc agtaccgcgt cgccaggacg cgatatccgt
      241 cgctgggctc cgccgcatca tagatcgact ttgcctgaac gaggggagcg ggctcgctag
      301 ccacgacctg tcgcgcgcat cgccctactc caaccccgcc ctgcggaatg cctcgtcgac
      361 ctcgcgcacg taatacgagg gatcgaacag cttcgtcaac ccttccttgg cgatggcgcg
      421 cgtcacctcg gcatcctcgc agagcaggtc gaggaactgc cgttcctcgc tccaggcccg
      481 catcgcgttg cgctgcacca gctcgtacgc ctcgtcgcgg ctgaggcctg catcgacgag
      541 cgcgagcaac acgcgttgcg agaagggcag accgtacgag agctcgaggt tgcgccgcat
      601 acgctccggg tacacgtgca gtccgcgcat cacgaacgtc atcaggtcga gggcgtagtc
      661 caacacaagg cacgcatccg ggaagatgat gcgctcggcg gacgaatggg agatgtcgcg
      721 ctcgtgccac aaggccacat tctcgagcga ggtgagggcg tacccccgca gcagccgtgc
      781 gaggccgcaa atgcgctcca ccttctccgg gttccgcttg tgcggcatcg aggaggatcc
      841 cttttgtccc ggcgagaagg gctcctccac ctcgcgcacc tccgtgcgct gcagtccacg
      901 gatctcggtc gcgaacttct cgaggctcga gccgatgagg gcgagggtgg cgacgaagtg
      961 ggcgtgacga tcgcggtgca cgatctggct cgataccggt tcgacggcga ggcccagctc
     1021 ggccagcgcc cgttcctcga cggcggccgg caccgaggcg tgggtgccca ccggtccgct
     1081 cagcttgccg accgcgatct gctcgcgcgc ttgttcgaga cgcgcgcgcc cccgctgaag
     1141 ctcgaccacc cacgccgcca gcttgaggcc gaacgtggtg ggctcggcgt gaaccccgtg
     1201 cgtgcggccc atctggagcg tgtgcttgtg ctcgcgtgcc ttctcggcaa cggcgacggt
     1261 gagcgcatcg agctcccgta gcagcaggtc cgatgcttgg accaactgga gcgccagcgc
     1321 cgtatcgacg acatcggacg aggtgagccc gtagtggacc cagcggcctt cgggcccgag
     1381 cgagtcggaa acggagcgaa ggaaggccgt gacatcgtga tgctgctcgc gctcgtaccg
     1441 gcgcacctcg tccagatcga atcgcgcgtt cgcctcgatg cacgccaagt cctcgcgagg
     1501 gatgatgccc gtcacgtcag cccaggcgcg cgcggcggcg atctccacgc ggagccactg
     1561 ccggtacttg ttttcctcgg cccagacggc gctcatctcc gggcgggagt atcgagggat
     1621 catgtctaca gctccccgaa ccctgccggc gccgcgatgt cacgccgcac gtgcgccccc
     1681 tcgaagcgaa tccggccgac gttatcgtac gcgcgcgccg ttgcctgcgc cacggtgtcg
     1741 cccaacgcca ccacggtcag cacccgcccc ccgctcgtca cgagttggcc tgaatcgttg
     1801 agcgccgtgc cggcgtggaa cacgagcaca tcatcgtcca ccgactcgag gccctcgatc
     1861 gggacgcctg tacgataggg gcccgggtaa ccgcccgaag ccatgaccac gccgaccgcc
     1921 gccttcggcg agacgcggag acgcgcctcg cccaggtcgc cggtagcaca cgcccatagc
     1981 agatcgagca ggtcgctctc tatgcgcggc agcagcacct gcgtttccgg atcgccaaag
     2041 cgggcgttga actcgatgac cttcggtcca tcgggcgtca gcatgaggcc ggggtagagc
     2101 aggccgcgaa acggcgcccc ctccgcagcg agggcagcga tggtgggctc ggtgaccgtc
     2161 cgctgcacgg tttcctctag ctcgggcgtc acgtcctcca ggggcgagta ggcgcccatg
     2221 ccccccgtgt tcggcccgcg atcgccatcc aatgcgcgct tgtaatcgcg ggcgaaggga
     2281 agctgaagaa catgcctgcc atcggacaag gcgtgcgcgc tcgcctcacg gccttcgagg
     2341 cgctcctcta cgaggacgcg cgcccctgcc gcaccgtact cacggtcgag catggcccgt
     2401 accaccgcct gctccgcctc ctcccgcgtc gccggcacgg tcacgccctt gccggaggcg
     2461 atgccgtcgg ccttcacgac cggcggcgcc tccaaggttc ggacgtaggc gagcgcttcg
     2521 tcgggatcat cgaagatgcg ggcagcggcg gtcgggatgc cgtgctggaa catcagctgc
     2581 ttcgcgaagg ccttgctcga ttcgattcgc gccgccccgg ctgtagggcc gaaggccgcg
     2641 atgccggctg cgaggagccg gtcgacgagg ccggtcgcaa gaggcgcctc gggccccaca
     2701 acgacgagat cgatgcgctc gccgcgtgcc aaggcgatga tctcgtcgtc ggagcgcacg
     2761 ctcggaacgg ccgcggctag ccgggcgatt cccgcgttgc ccggcgctac gaacagcgca
     2821 tcgagcttcg ggctttggcc gatcttccaa gccaacgcgt gctcgcggcc gcccgacccg
     2881 acgatcagga ctcgcacggc gagatgccct cacacgcccc ggcgtctgcg tcgccggcga
     2941 tccggcagca ggaacatgac caccggcgca gccgggccaa cggcgatgcc aagcccagcg
     3001 ccgagcggcc accaatagcc gcggcgctgc gccgcccagg cggcgacaag catgccagcg
     3061 acgtacaagg ccacgaggac cgcgatactc acgtcactcc cattcgatgg taccgggcgg
     3121 cttgctcgtg atgtcgtaga cgacgcggtt cacgcccggc acctcgttga cgatccgcga
     3181 cgagatgcgc gccatcaggt cgtaaggcag ccgcgcccaa tcggccgtca tcgcatcctc
     3241 cgacgtcacg gcgcgaatcg ccacgacgtg accgtaggtt cggaagtcgc ccatcacgcc
     3301 gaccgtccgg ctgtcggtga gcacggcgaa gctctgccac aactcatagt acaggcccgc
     3361 gcgccgaatc tcgtccatga cgacggcgtc ggcggagcgc agcacctcga gcttctcgcg
     3421 cgtgacctca ccgaggattc gaatcgcgag ccccggaccg gggaacggct gccggtacac
     3481 gatgtcgtcc gggaggccaa gctccacgcc gaccttgcga acttcatcct tgaacagatt
     3541 gcggagcggc tccacgaggc ggaacttcat gtccttcggc aggccgccca cgttgtggtg
     3601 ggtcttgatc ttggccgctg ccgcggtccc gctcgacgcg ctctcgatga cgtctgggta
     3661 cagggtgccc tgggcgatga agtcgacctg acccagcttt cccgcctcgc tctcgaacac
     3721 gcgaatgaac tcctcgccga tgcgtacgcg cttcgtctcg gggttggtga cttcggccag
     3781 gcaggcgagg aagcggtcct cggccttggc gtgcaccaag ttgatcccca tcgaccgccg
     3841 gaacgtctcg acgacgcgct ccggctcgcc ccgccgcagc aggccattat cgacgaagat
     3901 acaggtgagc cgatcgccga cggcccgatg caccagtgcc gccgtgacgg cagagtcgac
     3961 cccgcccgac agcgcacaga tgatccggcc atctgcctgc tcgcggatag catcgaccgc
     4021 ctcggagatg aagttcccag gcgtccacgt gccgctgcag ccgcagagcc gaaagaggaa
     4081 attgcgcagc atctcccggc cttgtggcgt atgcacgacc tctggatgga actgaatgcc
     4141 caccatgcct tgctcgttac ccatcgctgc gatgggcgag ttctccgagt gggcgagggc
     4201 ggcgaacccc gggggcatct cggtgattcg gtcgccgtgg ctcatccaca cgggcagcga
     4261 ggccgggagt ccctcgaaga ggccgatgtc actggctccc cggtgaatca cggcatgacc
     4321 atactcgcga tggtcactcg gggccacgcg accgcccatc tggtgcgcca agagctgcat
     4381 accgtagcag atgcccagca ccggcacacg agcctcgtac acatacggag gcgcgagcgg
     4441 cgcgtcgggc tcgtagacgc tcgccggccc ccccgagagg atgaacccct ttggtcgcag
     4501 ccgggcgatg cgctcccaag gcgcatcgtg aggcaccaac tcgcagtaga cgttgagctc
     4561 gcggacccgg cgggcaatga gcatcgagta ctgcgagcca aagtccagga cgacgatggc
     4621 ctccggctcc ttcgggggcg ccggggcagg ggcagcctct gcggcttcgc ccggcgcgcc
     4681 gcttgccagc tcgaggtacg ccgaaacttc gaggtcgtcc gtggtccgga ggcgtgggcc
     4741 cttcgaagct tcgttcgtca tcgtagcggt gagtgtcgaa cacaagaggg ccgcccgatg
     4801 gggcggcctc gtcgtcgtag gagcggtatc gtcgtgcagc ggctcatgcc gcatcgtagc
     4861 atcgccgcta tactgcgatc aaggagtcaa cggcgtatgc agatcgtcgc cgaggacgat
     4921 gcagcctcca tcgacctcgc agtgcaggcg ctccggcgcg ggcatctcgt cgccttcccc
     4981 accgacacgg tgtacggcgt cgccgcgctc gccgcggacg agcaggcgtg cagcgccctc
     5041 ttttcggcca agggtcggcc gccggagcga gccatcccga tcttcatccc gtcgatagac
     5101 ctgctggggg aggtcgcatc gggcttcggg gaggcagagc tgcgtctcgc gcgtgccgga
     5161 ctgcccggtc ccctcaccat cgtgctgcgc agggctccag cgtttcgcag cgccgccctc
     5221 gtcggcgacg acacggtagg ggtgcgcatt cccaaccatc gctgggtgtt ggcgctcctt
     5281 cgcaccctc
//
LOCUS       DATMBB010000130         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_569672, whole genome shotgun sequence.
ACCESSION   DATMBB010000130 DATMBB010000000
VERSION     DATMBB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569672"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2378
                     /locus_tag="VNL92_02775"
     CDS             <1..2378
                     /locus_tag="VNL92_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWO93663.1"
                     /translation="EVLVRVRSDALGSWYEHGARVLERSVDGGEAFFRLESSQGEEVL
                     DNLSSRVELARVSEVLRLYCKALTGTDIALAAASGLTDKHIGWMSNDLPSTEGTSIFL
                     PEAIEESDNKGENFQIYKVYATHQAGHLEFGSFWFRFGRPGAILGDAGRRRLAEAVPS
                     HRQALTDIEAFFDLFPDRQLAADLFAIVEDARIDAIVMREYGGIRRSFRAVQQSELAR
                     RPLPERMPLRQAFAEALVRLSLDEHAELRWPAQLQHVLWEAAEYLNCVRISGARVEDA
                     AEATIALYDLLKSVPNLSPEDLGFDADDWQDFQAPETSMDPSEMQEGGVGEGGEAAQF
                     AMSPDGEGDMPYESPEQVGFRGDFKPELVQLLMRLRQGGEQADGEMSQAPISPEMLQQ
                     LLEKSVEITLTDVAEGDLASSSGMFADNLMKEAEALQKEQERKKQKGGSEAGQGGPDE
                     NDQPLASEPKFFLYDEWDFRAGDYKPRWCRLMEQTLDEGDIDFYERTTREKATLIAQT
                     RRQFELLRPELFRKIKRLHDGEEFDLDAAIEYIAERRSGHSPNDKVYWRRNKVERDVA
                     VAFLLDMSASTDEEIEKRKPVYDEDEFDDDPRRYLSWWASRRAHEMLAPAKRIIDVEK
                     ESIVLLIKALETIGDAYGIYGFSGYGRENVEYYVIKDLQETFGERIKRRIDRITPIRS
                     TRMGPAIRHTTTKLLAGDAKVKILFLVSDGRPQDHGYGRDRTEKEYAIHDTKMALTEA
                     KRKGVTPFALTVDRNGHDYLKQMCDDMGYEVLADVEALPGRLPALYRMLTE"
     gene            complement(2406..>2549)
                     /locus_tag="VNL92_02780"
     CDS             complement(2406..>2549)
                     /locus_tag="VNL92_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWO93664.1"
                     /translation="MDRLRKARIDEADILAAARERQGLERLDQIKYAVLERSGGISIV
                     PRR"
BASE COUNT          495 a          793 c          836 g          425 t
ORIGIN      
        1 cggaggtgtt ggtgcgggtc cggtccgatg cgctcggctc gtggtacgag catggcgcgc
       61 gcgtgctgga gcgcagcgtc gatggcggcg aggccttctt ccgcctggag tcgagccaag
      121 gcgaggaggt gttggataac ctctcgtcgc gagtggagct cgcgcgcgtg tcggaagtgc
      181 tgcggctgta ttgcaaggcg ctcacgggca ccgatatcgc gctggccgcg gcgagcggcc
      241 tcacggacaa gcacatcggc tggatgtcga acgacctgcc ttcgaccgaa ggcacatcca
      301 tcttccttcc agaggcgatc gaggaatcgg acaataaggg cgagaacttc cagatctaca
      361 aggtctatgc gacgcaccag gcaggccacc tggagttcgg cagcttttgg ttccgcttcg
      421 ggcggcccgg cgccattctc ggcgacgcgg gccgtcgccg tctggccgag gcagtgccga
      481 gccatcgtca ggccctcacc gacatcgagg ccttcttcga tctcttcccg gaccgccagc
      541 tcgccgcaga cctgtttgcc attgtcgagg atgcccgaat cgacgctatc gtcatgcgag
      601 agtacggcgg cattcgccgc agcttccgtg ctgtgcagca aagcgaactg gcccgccgac
      661 cgttacccga acgcatgccg ctgcggcagg cgttcgcgga ggccctggtg cggctcagcc
      721 tcgatgagca tgcggagctt cgctggcccg cacagctgca acatgtgcta tgggaggccg
      781 ccgagtatct caactgcgtc cgcatatcgg gggcgcgcgt cgaggatgcc gccgaagcga
      841 cgattgctct ctacgacctg ctgaagtcgg tgcccaatct ctccccggag gacttgggat
      901 tcgacgcgga cgattggcag gatttccagg ctcccgagac gtcgatggac ccctcggaaa
      961 tgcaggaggg gggcgtcgga gagggtggcg aagcggctca gttcgcgatg agccccgacg
     1021 gcgagggcga catgccttac gagagccccg agcaggtcgg ctttaggggc gatttcaagc
     1081 cggaactcgt gcagttgctc atgcggttgc gtcagggcgg cgaacaggcc gacggcgaga
     1141 tgtcccaagc ccccatttcg ccggagatgc tgcagcagct cctggagaag agcgtcgaga
     1201 tcacgctgac ggacgtcgcg gaaggcgatc tggcgtcatc gagcggcatg ttcgccgaca
     1261 acctgatgaa ggaagcagag gcgctccaga aggagcaaga acgaaagaag cagaagggcg
     1321 gcagcgaggc cggccagggc ggccccgacg agaacgatca gccgctcgcc agcgagccca
     1381 agttcttcct ctacgacgag tgggacttcc gggccggcga ctataagccc cgctggtgcc
     1441 gcctcatgga gcagaccctc gacgagggcg acatcgactt ctacgagcgc acgactcgcg
     1501 aaaaggccac cctcatcgct cagacacggc gccagttcga gttgctccgt cccgagctgt
     1561 tccgcaagat caagcgcctg cacgacggcg aggagttcga cctcgatgcc gcgatcgagt
     1621 acatcgccga gcgacgttcg ggccactcgc ccaacgacaa agtgtactgg cggcggaaca
     1681 aggtggagcg tgacgtagct gtcgccttcc tgctcgacat gagcgcttcg accgatgagg
     1741 agatcgagaa gcgcaagcct gtctacgacg aggacgaatt cgacgacgac ccgagacgtt
     1801 acctctcgtg gtgggcaagc cgtcgagcgc acgagatgct ggcgccggcc aagcgcatca
     1861 tcgacgtcga gaaggagagc atcgtccttc ttatcaaggc gctcgagacg atcggcgacg
     1921 cgtatggaat ctacggcttc tcgggatacg gccgcgagaa cgtcgaatac tacgtcatca
     1981 aggatttgca ggagacgttc ggcgagcgga tcaagcgccg catcgaccgg atcacgccca
     2041 tccgtagcac gcgcatgggt cccgccattc gtcacacgac aacgaagctc ctcgcggggg
     2101 atgcgaaggt caagattctg ttcctcgtct cggacgggcg gccccaggac catggctacg
     2161 ggcgtgaccg taccgagaag gaatacgcca tccacgacac gaaaatggcg ctcacggagg
     2221 cgaaacgcaa aggcgtcacg ccattcgccc tcacggtcga tcgcaacggc catgattacc
     2281 tgaagcagat gtgcgacgac atggggtacg aggtgctggc cgatgtcgag gcgttgccgg
     2341 gacggctgcc tgcgctctat cgcatgctga ccgagtaggc tggttcgttc gtcggctcgg
     2401 cggacctacc gcctcggcac gatcgagatg cctccacttc gctccagcac ggcgtacttg
     2461 atctggtcca agcgctccaa tccttgccgc tcgcgagccg cagcgagaat atccgcctca
     2521 tcgatccgtg ccttgcgcag ccggtccat
//
LOCUS       DATMBB010000131         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_4975, whole genome shotgun sequence.
ACCESSION   DATMBB010000131 DATMBB010000000
VERSION     DATMBB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4975"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1193)
                     /gene="nadC"
                     /locus_tag="VNL92_02785"
     CDS             complement(288..1193)
                     /gene="nadC"
                     /locus_tag="VNL92_02785"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWO93665.1"
                     /translation="MTSVEGVERDWMVATPPDATLVRRLARMALDEDGAWYDVTTMAL
                     VPPQQRGTAVVVAREAGVLCGAPGVAAVYEALGGEVRCEPLRTEGSAFHAQDELMRLT
                     GPLARILQGERVSLNFLQRLSGIATLTRSFVDAVVGTNVRIVDTRKTTPGLRLLEKYA
                     VRVGGGANHRFGLHDGVLVKDNHIAAGRARGLTLADIVRQARRNAPHPLRIEVEVTSV
                     AEAHEALEAGAEALLLDNMTSVEMAEVARLVRGRVPLEASGGITLERVRDVAEAGVDL
                     VSVGALTHSARAVDLSLEVVPETSL"
     gene            complement(1190..>2489)
                     /locus_tag="VNL92_02790"
     CDS             complement(1190..>2489)
                     /locus_tag="VNL92_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWO93666.1"
                     /translation="LVRFGVPFDTLQGELALGREAAHSRSRILHAGGDRTGAFIELAL
                     AARARADGITILEHRFVERIIVDGGRVTGVEALATEGLGERTQFACSHLILATGGAGQ
                     LYGITTNPPVATGDGIALAYWAGAELADLEFVQFHPTALRMPGVPTFLISEAVRGEGG
                     ILRSLDGRPFMAAEHPDADLAPRDIVARAIAREMERSGSTHVLLDVTHLDPATTAARF
                     PQIYRYCLEHGLDITRESIPVSPAAHYMMGGVRTDTWGRTSIPGLYACGEVACTGVHG
                     ANRLASNSLLETVVFAQRAVEASVGGVESDWPAAESDNFDLPPPAPIEPAPPYDLATL
                     QRIMWQHVGIERSGGGLLQAREHLAAMAMTAPWERGDRASLELRSLVTTARLVTEAAL
                     RRTESRGAHFRSDHPRSRQSWLRHNIIRRMPSGSAEGPHP"
BASE COUNT          390 a          843 c          886 g          370 t
ORIGIN      
        1 gaagccaaga cgtccgtcat ctccattgcg gacatgccgc cgccgcggcg cgagggtccg
       61 accgggcgcg gagcaaggcc ccgttcgccc atccccgccc ctcggggagg ccgcagccgg
      121 cggtccgtgg aagagcctgt cgtcggagat gcctcgagtg cgcctgagcc gccctcggag
      181 gctggtgagc caggcgaagg atccgtgcct tctggcacgg atgaccaacg acgccggaag
      241 cgccgccgcc gtcgccgtgg aggcggaagc tcctagctag ggacgcctca taagctcgtc
      301 tcggggacga cctcgagcga gaggtcgacg gcgcgagcgg aatgcgtgag cgcgcccacg
      361 gagacgagat cgacgcccgc ctcggccaca tccctcacgc gttccaaggt tatgccgccc
      421 gatgcttcga gggggacgcg gcccctgacg aggcgggcca cctccgccat ttcgaccgac
      481 gtcatgttat cgagcaggag cgcttccgca cccgcctcga gcgcctcgtg ggcttccgcc
      541 acgctggtca cctcgacctc gatgcgaagc ggatgcggag cgttgcgccg ggcttggcga
      601 acgatatcag cgagcgtgag accgcgggcg cggccggcag cgatgtggtt gtccttgacg
      661 agcaccccat cgtggaggcc aaagcggtgg ttggcgccac ccccaacccg gaccgcgtac
      721 ttttccagca ggcgcaggcc cggcgtcgtc ttgcgcgtgt caacgatgcg tacgttggtt
      781 ccgacgacgg cgtcgacgaa gctgcgcgtc agcgtggcga tgccggagag ccgctgcagg
      841 aagttgagcg acacgcgctc gccctgaaga atgcgcgcga gggggccggt gaggcgcatc
      901 agctcgtcct gagcatggaa cgccgagcct tcggtgcgga gcggctcgca gcgcacctcg
      961 ccgcccagcg cctcgtagac agctgcgacg cccggagcac cgcacagaac gcccgcctct
     1021 cgcgccacaa cgacggccgt accgcgctgt tggggtggga cgagtgccat cgtcgtgacg
     1081 tcgtaccagg cgccatcctc gtcgagggcc atccgcgcga ggcggcggac gagagtggca
     1141 tcgggcggcg tcgcgaccat ccagtcccgc tcgacgcctt cgaccgatgt catggatgcg
     1201 ggccttccgc cgatccgctc ggcatacgcc ggatgatgtt gtgccgaagc cacgattggc
     1261 ggctgcgagg gtggtcggac cggaagtgcg cgccgcggct ctctgtccgg cgcaacgccg
     1321 cttcggtgac gagccgggcc gtcgtgacca gcgatcgcag ctccagcgag gcccggtccc
     1381 cgcgctccca tggtgccgtc atggccatgg ctgcgaggtg ttcgcgggct tgcagcaggc
     1441 cgcccccgct tcgctcgata cccacgtgct gccacatgat ccgctgcagt gtcgcgaggt
     1501 cgtacggcgg agcaggctct atcggggccg gaggcggaag atcgaagtta tcggattccg
     1561 ccgccggcca gtcgctttcg actccgccga cggaggcctc gacagcccgt tgagcgaaca
     1621 cgaccgtttc gagtaacgag ttgctcgcaa ggcggtttgc gccgtgcacg ccggtgcatg
     1681 cgacttcgcc gcaagcgtac agtcccggaa tgctggtgcg tccccacgta tcggtccgca
     1741 ctccgcccat catgtaatgg gccgccggcg agacggggat cgactcgcgc gtgatgtcga
     1801 gcccgtgctc aaggcagtag cggtagattt gcgggaaccg cgcagccgtg gtcgccggat
     1861 cgaggtgcgt gacgtcgagc aggacgtggg tggagccgga gcgctccatt tcgcgcgcga
     1921 tggcgcgggc gacgatgtcg cggggcgcga gatcggcatc agggtgctcc gctgccatga
     1981 agggacgtcc atcgaggctg cgcaggatgc cgccttcgcc gcgcaccgcc tccgatatca
     2041 ggaacgtggg tacgccgggc atccgcaagg cggtgggatg gaactgcacg aactccaagt
     2101 ccgccagctc ggcgcccgcc cagtaggcga gggcaatgcc atcgccggtg gcgacggggg
     2161 gattggtggt gatgccgtac aactgcccgg cgccgcctgt cgccagaatc agatgcgagc
     2221 aggcaaactg cgtccgttcc cccaggcctt ccgtcgcgag cgcctcgacg cccgtgactc
     2281 ggccgccatc gacgatgatg cgctcgacga agcggtgctc caggatcgtg atgccgtccg
     2341 ctcgggcccg ggccgcgagc gcgagctcga tgaaagcgcc cgtcctatcg cctccggcgt
     2401 ggaggatgcg cgaacggcta tgcgccgcct cgcggcccag cgcgagctcg ccctggagcg
     2461 tgtcgaaggg gacgccgaag cggacgagg
//
LOCUS       DATMBB010000132         4679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_324137, whole genome shotgun sequence.
ACCESSION   DATMBB010000132 DATMBB010000000
VERSION     DATMBB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324137"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VNL92_02795"
     CDS             complement(<1..280)
                     /locus_tag="VNL92_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93667.1"
                     /translation="MVLVNSRRRMAGLAILALITLLALIFASSVGGQSPQGGRIGLAH
                     NPFLPHERDHVLVRLDFGSNAFRVSPGAQFIFDRWFRVPVLPNETPSEA"
     gene            complement(416..1213)
                     /locus_tag="VNL92_02800"
     CDS             complement(416..1213)
                     /locus_tag="VNL92_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93668.1"
                     /translation="MRRRLSLVMTLTLLLVAALAAACGDDDDDDGDGDGDASGTPVIF
                     APPTLSSLDAVAVSTDQQNLWVDFTFDPSTGIPSLATCAATAADGSTYLAATFAELES
                     DKARAVIPLGAAPDGDVSVRCEGGGDTSLAQQTTIARDPTKVKTGAEVRVYNVPDRVL
                     DRNQPTYVQGERTQSFQFTWYRSGFEWTTGQGQPLHFSETEPGVFTAEADPFEELHQE
                     FTVRFEGNDILLHGRAEWDNGRVEEYDDRWTFVSSATPTPTPEPRPG"
     gene            complement(1288..1905)
                     /locus_tag="VNL92_02805"
     CDS             complement(1288..1905)
                     /locus_tag="VNL92_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HWO93669.1"
                     /translation="MTRLLASKRALALSLVVAGAAATVFALIAPGAPSAQGGASPSAI
                     DVTPNGDTWVPGQNGVVLSGAGPIDPFVALIEAESGREVEAVWALLGGSYVFEVPGFD
                     GSTFPDLPGPVLAAFAVLGDPASVAPPPVQPTTEPTVEPTIAPTTPPETEPTMEPTQS
                     PSDGAEPYYANCEEVREAGADPIFILEPGYGPHLDPDGDGIGCYP"
     gene            2177..3364
                     /locus_tag="VNL92_02810"
     CDS             2177..3364
                     /locus_tag="VNL92_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93670.1"
                     /translation="MASMTSRARRASVYGLAALGALQVYRKAFYGMTRRRVIRLERPV
                     NYDPDLLYPEPLGKPIYLVKPENDTTLLFLSGFRTLTMPGQHLDWFRELHEQHGINIL
                     APVFGIQSLPFDQRMVGTTVERDVREVMQVLDAYRSTLPADHRIVCSCISYGAIPSLA
                     IAAMRDVSDFILTGPAPIITPPGPTTRTPSIVRRLKNPVLRPIAASLLKRLHNPESKG
                     MRLTLAIAGQLVPTMARRRLESGWDIEHEERRQYAYDNLPLPADVPLADAKSMLTGIA
                     LTRESYLPRITGKRFLIAWGELDSLVTPEQNEALADELEQLGNEVIRRGYENSGHLVL
                     MDGELEQVQDDILEFIRPGASLFAKSKPAAPEVAEPAPRRAKAPTAAAAAKASARSNG
                     AQT"
     gene            3653..>4679
                     /locus_tag="VNL92_02815"
     CDS             3653..>4679
                     /locus_tag="VNL92_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93671.1"
                     /translation="MIQRTKRTRRAAVGALALLGAARLYRAAFYHQARRRVLKVERRV
                     PYDPDLLYPDPMGRPIYLVDPRNDTTLLFLSGYRMLSMPGEHLDWFRELHGRGINVLA
                     PLVGIQSLPFDHRIVGTTLERDVREAVQVLDAYRSTLPSDHRIICASMSYGSIPSFAI
                     AALRDVSDFVLLGPASVFAPTGRSAHSSATLQRMRNPTLRGWALALRPRALSQQDPMM
                     KVVTRIVEGLAPLLVRTRVESGWDIADPERRAEAHQRFLLPADVPMSDMWMLERSMRL
                     VRESYLPRITGKRFLMAWGELDSVVMPEQNEALADQLERLGNDVIRRPCERSGHLVLM
                     DGERDQVM"
BASE COUNT          816 a         1601 c         1487 g          775 t
ORIGIN      
        1 tggcctccga tggcgtctcg ttcggcagga cgggcacgcg gaaccagcga tcgaagatga
       61 actgcgcgcc ggggctcacg cggaaggcat tggacccaaa gtcgaggcgc acgagcacgt
      121 ggtccctctc gtgagggagg aacggattgt gcgccaagcc gatgcgtccg ccttgaggcg
      181 actgtccgcc gacactcgac gcgaagatga gcgcgagcag tgtgatgagc gcaagaatcg
      241 ccaaaccggc cattcgccgg cgagaattga ccagaaccac cggtgtcccc cttctcgttc
      301 cccgcgagac tctcatagct taacggatcc gccgtcatct atctacggcc ggcgcgaacg
      361 gcaatcgttc gatctgattg tcggcagatg ggggccgtct cgggaggcgc cgggtctagc
      421 ctggacgcgg ctcgggtgtc ggcgtgggcg tggcgctcga aacgaaggtc cagcggtcgt
      481 cgtactcttc gacacgaccg ttgtcccact ccgcccgtcc atggagcagg atgtcgttcc
      541 cctcgaatcg taccgtgaac tcttggtgca gctcctcgaa cggatcggcc tcggccgtga
      601 acacaccggg ttcggtctcg ctgaagtgga gcggctgccc ctggcccgtc gtccactcga
      661 acccgctccg gtaccaggtg aactggaacg attgcgtgcg ctctccctgg acgtatgtcg
      721 gctggttgcg gtcgagcacg cggtccggga cgttgtagac gcgtacctcc gcccccgtct
      781 tcaccttcgt cggatcgcgc gcaatcgtcg tctgctgcgc aagggaggta tcgccgccgc
      841 cctcgcagcg cacggatacg tcgccatccg gcgccgcgcc gagcgggatg acggcacgag
      901 ccttgtctga ctcgagctcc gcgaacgtgg cggccagata cgtcgaacca tccgccgccg
      961 tcgcggcgca cgtggcgagg gaagggatgc ccgtcgacgg gtcgaaggtg aagtcgaccc
     1021 acaagttctg ctggtcggtc gacacggcca cggcgtcgag cgaggagagc gtgggcggcg
     1081 cgaagatcac gggcgtgccc gaagcgtcgc cgtcaccatc accatcatcg tcatcgtcat
     1141 cgccgcaagc cgcagcaagc gcggcgacca gcagcagtgt cagcgtcatg acgagagaaa
     1201 gcctgcggcg caaggtcgtg ttcctcctcg ggggcgagac gagcctcacc tccttacgtc
     1261 gtgtgacagc cccgggacgg atgctgctta cgggtagcaa ccgatgccgt caccatccgg
     1321 gtcgaggtga gggccgtagc ccggctcaag gatgaagatc gggtccgcgc cggcctcccg
     1381 cacttcctcg cagttcgcgt agtacggctc ggctccgtcc gagggtgatt gcgtcggttc
     1441 catggtgggc tccgtctcgg gcggagtcgt cggcgcaatg gttggctcga cagtgggctc
     1501 cgtcgtgggc tgcaccggag gtggcgcgac cgaagcggga tcgccgagga cggcgaaggc
     1561 cgccaacacg ggccccggca gatcggggaa ggtgctgcca tcgaagccgg gtacctcgaa
     1621 cacgtacgac ccgccaagca gcgcccacac tgcctccacc tcgcggccgc tttccgcctc
     1681 gatgagcgcg acgaaggggt cgatgggacc ggcgccgctc aggacgacgc cgttctgccc
     1741 agggacccac gtgtcgccgt tgggcgtgac gtcgatggcc gaaggcgacg cacctccctg
     1801 cgcggagggg gcgcccggcg cgatgagtgc gaagacggtc gcagccgctc cggctaccac
     1861 cagcgatagt gccagggcgc gtttcgaggc gagaagacgt gtcatcgtgc gttcccttcc
     1921 cgacgggcga agtctcgcgc gccgtgaggg cggcgcggtg ctcgtcctta ggtaccgcgc
     1981 cgcgtcgctt gtgtcaaccc gagccctcac cggcgcaggg tccggtggga gccgcatcaa
     2041 gtctttcgag actcgtacct atccttgaca tttacgtaac atggctagtc tactgtaggt
     2101 cttatcggga agttccctaa gggtcaggag ggggctcctt agggacatca cctacggatg
     2161 ggagagggaa agcaccatgg catccatgac aagccgggcg cggcgcgcct cggtctatgg
     2221 gctggcagcg cttggcgccc tgcaggtcta tcggaaggcg ttctacggca tgacccgccg
     2281 acgcgtcatc cgcctcgaac ggccagtcaa ctacgacccc gacctgctgt accccgagcc
     2341 gctcggaaag cccatctatc tggtaaagcc ggagaacgac acgacgctgc tcttcctgag
     2401 cggcttccgt acgctcacca tgccgggcca gcacctcgat tggttccgcg agctgcatga
     2461 gcagcacggc atcaacatcc tcgcccctgt cttcgggatt cagagcctgc cgttcgatca
     2521 gcgcatggtc ggcacgacgg tcgagcgcga cgtgcgggaa gtgatgcagg tcctcgacgc
     2581 ctaccgatcg accctgcccg ccgaccaccg catcgtctgc tcgtgcatct cgtacggcgc
     2641 catccccagc ctggcgatcg ccgccatgcg cgatgtgtcc gacttcatcc tcaccggccc
     2701 ggctcccatc atcacgccgc cggggcccac gacgcgtacg ccgtccatcg tccgcaggct
     2761 caagaacccc gtactgcggc ccatcgcggc atcgctgctc aagcggctcc acaacccaga
     2821 gagcaaaggc atgcggctca cactggccat cgcgggtcag ctcgtgccca cgatggcccg
     2881 gcgacgcctc gaaagcggct gggacatcga gcacgaggaa cgacgccaat acgcttacga
     2941 caatctaccg ttgcccgccg atgtgccgct ggccgacgcg aagagcatgt tgaccggcat
     3001 cgcactgacg cgcgagagct acctaccgcg catcacgggc aagcgattcc tcatcgcttg
     3061 gggcgagctc gattcgctcg tgacccccga gcagaacgag gcgctcgcgg atgagctgga
     3121 gcagctcggc aacgaggtga tccgccgcgg ctacgagaac tccggacacc tcgtgctcat
     3181 ggacggcgaa ctcgagcagg tgcaagacga catcctcgag ttcatccggc caggcgcatc
     3241 cctcttcgcc aagtcgaagc cggctgcgcc ggaagttgcc gagccggcgc cacggcgagc
     3301 gaaggcgccg accgctgccg cggcggccaa agcgagcgcc cgctcgaacg gcgcccaaac
     3361 atagcacgca cgcgctacat gctttcgtcc atcggccgcc tgcgggaaga ggcggcggat
     3421 ggacgtagct atatccagtc gtgcatccgt cgcatgcggg aagaggcggc ggctcgcggg
     3481 tccaagacca cggcagggct agagtcgcgc attgctacaa aattcgccct gaaccttgac
     3541 attctcacga cttcagcggt ataacgtccg gacatcccac gggcttccct catggcaagt
     3601 tcctgggcac ccacccaccc gatataggaa tctgtgaggg agaacgcgtc tcatgatcca
     3661 aagaaccaag cggacccggc gggctgccgt cggagccctc gcactgctcg gcgcggcccg
     3721 tctttatcgc gctgccttct accaccaggc ccgccgccgc gtcctgaagg tggagcgtcg
     3781 cgtcccatac gatccggacc tcctgtaccc ggacccgatg ggcaggccga tctatctcgt
     3841 cgaccctcga aacgacacca cgctcctgtt cctcagcggc tatcgcatgc tgtccatgcc
     3901 gggcgagcac ctcgattggt tccgcgagct ccacggccgt ggcatcaacg tgctcgcacc
     3961 ccttgttggc atccaaagcc tgcccttcga ccaccgcatc gtcgggacga cgctcgaacg
     4021 cgatgtgcgc gaggccgtcc aagtcctcga tgcctaccga tccacgctgc catcagacca
     4081 ccgcatcatc tgtgcgtcca tgtcgtatgg ctcgatcccg agcttcgcga tagccgccct
     4141 ccgtgacgtg tccgacttcg tgctcctcgg gcctgcgtcg gtctttgccc ccactgggcg
     4201 ctcggcacac agctcggcga cgcttcagcg gatgcgcaac ccgactctcc gcggctgggc
     4261 cttggccttg cgcccacgcg ccctcagcca gcaggatccg atgatgaagg tcgtcacccg
     4321 catcgtcgag ggccttgcgc ccctcctcgt gcggactcgc gtggagagcg gctgggacat
     4381 cgcagacccc gagcgccggg ccgaagccca tcagcgcttc ttgctccccg ctgacgtgcc
     4441 tatgagcgac atgtggatgc tcgagcggag catgcgactc gtgcgcgaga gctatctgcc
     4501 acgcatcacg gggaaacgat tcctcatggc ttggggcgag ctggattccg tcgtgatgcc
     4561 tgagcagaac gaggcgctcg ccgatcaact cgaacgactc gggaacgacg taatccgccg
     4621 cccatgcgaa cgctcggggc acctcgtgct gatggacggc gagcgcgacc aagtcatga
//
LOCUS       DATMBB010000133         2544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_64642, whole genome shotgun sequence.
ACCESSION   DATMBB010000133 DATMBB010000000
VERSION     DATMBB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64642"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VNL92_02820"
     CDS             <1..844
                     /locus_tag="VNL92_02820"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HWO93672.1"
                     /translation="AVDKENARRPCTQTMYTYGPRVGMQRILRLLRKYQIKASFYIPA
                     ITAERHPYMVEWIVADGHEIGCHGYTHDWPNWLDPDVEEEHLDRQIEVLSRMSGQRIR
                     GYISPGWEYSEQTLRLLRSRGIEFSGDWLGEDIPYYVDIDGEQTDFVAIPVNWTLDDA
                     PLYWFALFPPLSYGAPYAEPSRVLELWTSEFDALYEEGALFHLTLHPFLSGRGCRVKT
                     LERLIHYISLRPGVKFCTMAEVADMYRRVVPVERGRPGVWYPWREGQQVPKIVQVEGV
                     PIFT"
     gene            884..2002
                     /locus_tag="VNL92_02825"
     CDS             884..2002
                     /locus_tag="VNL92_02825"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HWO93673.1"
                     /translation="MPIIGSDPIPIVLVEGEPRERGRQHGELARDLVALSVERYMERF
                     AHFARLDPPGVRDLAAAFAPIIRDYDPDLLEEIEGVAEGAGFAREDLLAVNCRSEVMF
                     GKAVPECTSFGLNAEVTANGHTYVGQNWDWAPDIKETLILLVVKQEPKPTIVLLDEAG
                     MIGRMGLNDAGIGLATNTLISEQREIGVPYNMLLRGILNQRGMAQAIAALVRPKRAIS
                     ANYLIGHGDGQTIDIEASPIHVDTLVPRDGIITHGNHFEGPRLQGRDLSLERFPDSAY
                     RGCRLRDRLAKHSGSLTEADVQEALRDSFGHPDAICRAADPAQGRFDQLETVASIVID
                     ATERRFWLARGAPSENAYHEFLLADLAIGKVPTPVEAG"
     gene            2042..>2544
                     /locus_tag="VNL92_02830"
     CDS             2042..>2544
                     /locus_tag="VNL92_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWO93674.1"
                     /translation="MPIALTHAHLINGDGRSFDDATVVIAGGRIAKVATDAGSPSAEV
                     TIDLEGRALMPGMIDLHVHMVGGDKAMGFGDEATTFKMSDSVIKATLDGVEAARKTLH
                     AGITTVREIAARDYIDVYLKRAQATGQITGPRMLATGPGVFMTGGHGSFLQPGHEADG
                     VDAMVRRV"
BASE COUNT          474 a          825 c          822 g          423 t
ORIGIN      
        1 tgccgtggac aaggagaacg cgcgccggcc atgcacgcag acgatgtaca cgtacggccc
       61 ccgtgtcgga atgcagcgca tcctgcgcct ccttcgcaag taccagatca aggcaagctt
      121 ctacatcccc gccatcacgg ccgagcgaca tccctacatg gtcgagtgga ttgtggccga
      181 cgggcacgag atcggctgcc atggctacac ccacgactgg ccgaactggc tcgatccgga
      241 cgtcgaggaa gagcaccttg atcgccagat cgaggtgctt agccggatga gcggccagcg
      301 cattcgaggc tatattagcc ctggatggga gtacagcgag cagacgcttc ggctgcttcg
      361 ctctcgtggg atcgagtttt ccggcgactg gctcggcgag gatatcccgt actacgtaga
      421 catcgacggc gagcagaccg acttcgtcgc gatccctgtc aactggacgc tggacgacgc
      481 gccgctctac tggtttgcgc tgtttccgcc cttgagctac ggggctccct acgctgagcc
      541 ctcgcgggta cttgagctct ggacgagcga gttcgacgca ctctacgagg agggggccct
      601 cttccacctc acgctgcatc cgttcctgag cggacgggga tgccgcgtca agacacttga
      661 acggctcatc cactacatct cgctgcggcc cggcgtgaag ttctgcacga tggccgaggt
      721 agcggacatg tacaggcggg ttgtccccgt cgagcggggc cggcccggcg tctggtatcc
      781 gtggcgcgag ggccagcagg ttcccaagat cgtccaagtc gaaggcgtcc ccattttcac
      841 gtaggtggca gccggatccg gatcgaaggg aaggacgaac gatatgccga tcattggatc
      901 tgacccgatt ccgatcgtgc tcgtagaggg cgagccccgt gagcgtggac ggcaacacgg
      961 cgaacttgcc cgcgacctgg tggcgctgag cgttgagcgc tacatggagc gcttcgcgca
     1021 cttcgcgaga ctggatccgc ctggagtcag ggacctcgct gcggcgttcg cgccgatcat
     1081 tcgcgactac gaccctgact tgctcgagga gatcgagggt gtcgctgagg gggctgggtt
     1141 tgcccgtgag gacttgctcg ccgtgaattg ccgctcggag gtgatgttcg ggaaagctgt
     1201 ccccgaatgc acctccttcg ggctcaacgc cgaggtgacg gccaacggcc acacctacgt
     1261 cggacagaac tgggattggg cgcccgacat caaagagacc ctgatcctgc tcgtcgtcaa
     1321 gcaggagccg aagcccacca tcgtgctgct cgacgaggca ggaatgatcg ggcgcatggg
     1381 cctcaacgac gccggcatcg gtctggccac caacacgctg atctcggagc agcgcgagat
     1441 cggcgtgccc tacaacatgc tgctacgcgg aatcctgaac cagcgaggca tggcgcaggc
     1501 aatcgcggcg ctcgtccggc cgaagcgagc gatctcagcg aactacctaa tcggtcacgg
     1561 cgacggccag acgatcgaca tcgaggcctc cccgatccat gtcgacaccc tcgttccgcg
     1621 cgacggcatc atcacgcacg gaaaccactt cgaggggccg cgtttgcagg gtcgcgatct
     1681 cagccttgag cggttcccgg actccgccta tcgaggatgc aggctccgag accggctcgc
     1741 gaagcactcg ggatccctga ccgaagccga tgtccaggaa gcgctgcgcg actccttcgg
     1801 ccacccagac gcgatctgtc gcgcagccga tccggcccag ggccgcttcg atcagctcga
     1861 gaccgtcgcc tcgatcgtca tcgatgcgac ggagcgccgc ttctggctgg cacgaggggc
     1921 tccttccgag aacgcgtacc acgagtttct gctggcggat ctcgccatcg gcaaggttcc
     1981 gacgccggta gaggcggggt gaccacccga gagggccgga ttacacgagg aggactaggc
     2041 agtgccgatc gcgctcacac acgctcatct gatcaacggc gacggccgaa gcttcgatga
     2101 cgccacggtc gtcattgccg gcggccgcat cgcgaaagtc gcaaccgacg ctggatcgcc
     2161 cagcgccgag gtcaccattg atctcgaggg ccgggcgctg atgcccggga tgatcgatct
     2221 ccacgtccat atggtcggcg gcgacaaggc catgggcttc ggcgacgagg cgacgacctt
     2281 caaaatgagc gactcggtca tcaaggccac gctcgacggc gtcgaggcgg cgcgcaagac
     2341 gctacacgcg ggaatcacga ccgtccgaga gatcgccgcc cgcgactaca tcgacgtcta
     2401 cctcaagcgc gcccaggcca ccggacagat cacaggccct cgcatgctcg cgaccggccc
     2461 gggcgtgttc atgaccggcg ggcacggctc gttcctgcag ccgggtcatg aggccgacgg
     2521 tgtcgacgcg atggtgcggc gggt
//
LOCUS       DATMBB010000134         3383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_323932, whole genome shotgun sequence.
ACCESSION   DATMBB010000134 DATMBB010000000
VERSION     DATMBB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323932"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VNL92_02835"
     CDS             <1..1514
                     /locus_tag="VNL92_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93675.1"
                     /translation="KHIQDRLKLWGRALQGVEPYRIEFAEEPGLTGLTDFSNHLIKVN
                     PEMIAGLKPREGYEITKAVLCHEAGHRRFTAPAELSWVVHMVANILEDQRIESLIMEE
                     FAGTRPLIRRLTEEMYRQSPELDATDDPGQVVAAALQWRWAVRLGVGLKGELSEVNKE
                     RWEQVRPMAEEAWVAATSEEVNTIAWRIVQILGLNETQIPSWVVTALDRCVGHRKRDD
                     TTESKAPAAAKPFDAESDGGEGRPAPFDGEVVPNSKRVGRDYPIEPMPYAELEALAMP
                     MARELIDELTFIPANDEPEPSDRGGSLSVRQYLRERERPFLVRDGERLAPPVMAVRIV
                     IDHSTSMNHATAGRTRIQRAAEAAMMLHLAFTELGTEHGISVMPQQVHLADLTTGEPG
                     KALIAGLVPAKTGYEDLAVAIRARSDELLSVVADIRLLFVLHDGYPNDADKAKSLCQS
                     LKGNVEVIGLLLDPDEGTQSAMREIFGADRLVACASKDLPQKLAAMLEAIRGV"
     gene            1634..2377
                     /locus_tag="VNL92_02840"
     CDS             1634..2377
                     /locus_tag="VNL92_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWO93676.1"
                     /translation="MIAIANQKGGVGKTTTTLNLGVALARENQRVLLVDLDPQAALSV
                     SAGIAVQRLPTTVYQVLTGESEAHEAIVPTRLGPDILPAQIELAAAELELTSQIGRER
                     FLADALQHVASQYDFILIDHPPSLGILAINGLVAAPEVIIPVQCEFLAAKAIALLLDT
                     IERIRSKLSPTLRVLGILPTMYNARTLHAQEVLDELRATFNVPVFPFVVKHSVRFKEA
                     PAAAMSILDYAPDQDGAAAYRALAKELLK"
     gene            2623..2925
                     /locus_tag="VNL92_02845"
     CDS             2623..2925
                     /locus_tag="VNL92_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93677.1"
                     /translation="MRSEQSKQADAPATDIEGRRRRLRELVFRDHPKDVGYRFAQDEL
                     DALRDIEYELDVQRGIKVTRNDIVRLGLNQLIEDYRQRGDDSVLVEVFKEERSRGR"
     gene            2922..3362
                     /locus_tag="VNL92_02850"
     CDS             2922..3362
                     /locus_tag="VNL92_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93678.1"
                     /translation="MNEMSFVPRSELNVREIFAQRAERLGYRIIESRAAFPDYLLEHE
                     GERILAEAEFRTSDFLRHRHDLARCDLVIVWEHDLGSMPIPVLELKGNALHVMKRRGR
                     PPQSTSRWVNRRAFWARVSASKASAVQRTPLDHPGDPKDPPQSD"
BASE COUNT          692 a         1098 c         1043 g          550 t
ORIGIN      
        1 ggaagcacat ccaggaccgc ctgaagctct ggggacgcgc gttacagggc gtcgagccct
       61 accggatcga attcgcggaa gaacccggcc tgaccggtct cacggacttc agcaaccacc
      121 tcatcaaggt gaacccagag atgatcgccg gtctcaagcc acgggaggga tacgagatca
      181 cgaaggccgt cctctgccac gaggcggggc atcgccgctt cacggcgccg gcggagctca
      241 gctgggtcgt ccacatggtc gcgaatatcc tcgaggacca gcgcatcgag agcctgatca
      301 tggaggagtt cgccgggacg cggccgctga tccgccggct caccgaggag atgtaccgac
      361 agagcccgga gctcgacgca accgatgacc cgggccaggt ggtggccgcc gcgctgcagt
      421 ggcgctgggc cgtgcgcctg ggcgtcgggc tcaaaggaga actatcggaa gtcaacaagg
      481 agcgctggga gcaggtgcgc ccgatggcgg aggaggcctg ggtggccgca acaagtgagg
      541 aggtcaacac gatcgcctgg cgcatcgtgc agatcctcgg tctgaacgag acgcagatcc
      601 cgtcgtgggt ggtcacggcg ctcgaccgct gcgttgggca tcgcaaacgc gacgacacga
      661 ccgagtcgaa agcgcccgcg gcggcgaagc cgtttgatgc cgaatcggat ggtggcgaag
      721 gacggccggc gcccttcgac ggagaagtcg tacccaacag caaacgcgtg ggccgcgact
      781 atcccatcga gccgatgcca tatgccgagc ttgaagcctt ggccatgccg atggcgcgcg
      841 aactcatcga tgagctgacc ttcatcccgg ccaacgacga gcccgagccg tcagaccgag
      901 gcggttccct ctctgtgcgt caatacctgc gcgagcggga acggccattc ctcgtgcgag
      961 acggggagcg cctggcaccg cccgtcatgg ccgtgcgcat cgtcatcgac cactcgacgt
     1021 cgatgaacca cgcaaccgca gggcggacgc gcatccagcg tgcggcggag gcggcgatga
     1081 tgctgcacct ggcgttcacc gaactcggga ccgaacacgg catctcagtc atgccccagc
     1141 aggtacatct tgcggatctc acaacgggtg aacctggcaa ggcgctcatt gccggcctgg
     1201 ttccagcgaa gaccggctac gaagacttgg ccgtcgccat tcgagcgcgc agcgatgagc
     1261 tgctttccgt cgtcgccgac atccgcctgc tcttcgttct gcacgacggc tatccgaacg
     1321 acgccgacaa ggcgaagtcg ctgtgccagt cgctcaaagg aaacgtagaa gtgatcgggc
     1381 tgttgcttga tccggacgaa ggaacgcaga gcgccatgag ggagatcttt ggcgcagatc
     1441 ggctcgtcgc ctgtgcatcc aaggacctcc cgcagaagct cgccgcgatg cttgaagcga
     1501 tccgcggcgt ctaatcgaac ggggtatgga ccgcttggcc cctgccccgt tcttccatcg
     1561 actgcgctgc caccggcgac tacgcacgat ttgacggccg tgagacaata ggcgtataga
     1621 gacaacagcc cgcatgattg ccatcgccaa ccaaaagggc ggcgttggca agaccacaac
     1681 gacactcaat ctcggcgttg ccctggctag ggagaaccaa cgcgttcttc tggtcgattt
     1741 ggacccgcag gccgcgctct ccgtgagcgc cggcatagcc gtccagaggt taccgaccac
     1801 cgtttaccaa gtgctcacag gagagagcga ggctcatgag gcgatcgtgc cgacgcgtct
     1861 cgggcccgac atcttgccgg cgcagatcga actcgcggct gccgagctgg aactcacctc
     1921 tcagatcggg cgcgaacgct ttctcgccga cgcgttgcag cacgttgcgt cgcaatacga
     1981 cttcatcctc atcgatcatc cgccctctct cggcattctc gccatcaacg gactcgtcgc
     2041 cgcacccgag gtgatcatcc ccgttcaatg tgaatttctg gcggcaaagg cgatcgccct
     2101 gcttcttgat acgatcgagc gaattcgcag caagctcagc ccgacgctcc gcgttctcgg
     2161 catccttccg acgatgtaca acgcccgaac gctgcacgcc caagaagtgc tcgatgagct
     2221 ccgcgcaacg ttcaatgtgc ccgtgttccc cttcgtcgtg aagcacagcg tgcgcttcaa
     2281 ggaggcgccg gccgccgcca tgtccatcct cgactacgca ccggatcagg acggcgcggc
     2341 cgcataccgc gcgctggcaa aggagctact caaatgaaga accgcgtgtc gatcaagggc
     2401 agaggccttg gcgcattctt cggcgacgct gccagcaacg accctccgag ccaggaagcg
     2461 acgcatccgc cgactacgcc agccggtaga ggcacgagct ctccaagcca gctagcgagc
     2521 gagctagcta gctcgccagc tcggcagcca gcaagccagc ccgctatcga cccagcgagc
     2581 cagccagcca gccagcctgc aagctcgcca gcccgcgaga tcatgagatc cgaacagtcc
     2641 aagcaggcgg acgcaccggc caccgatatt gaaggccggc gtcgtcgatt acgcgagctc
     2701 gtctttcgag atcacccaaa ggatgtcgga taccgcttcg cgcaggacga gctggacgca
     2761 ctgcgtgaca tcgagtacga gctcgatgtg cagcggggca tcaaggtcac gaggaacgat
     2821 attgtcagac tgggtctcaa ccaactgatt gaggactacc gccaacgtgg agacgacagt
     2881 gtgctcgtgg aggtcttcaa agaggagagg agccgagggc gatgaacgaa atgtccttcg
     2941 tccctcgaag tgagctgaac gtccgtgaga tcttcgcgca gcgcgcggag cgcctgggct
     3001 atcgcatcat cgagagccgc gcagcgtttc cggactatct cctcgagcat gaaggggagc
     3061 gaatcctggc ggaagcggag ttccgtacat ccgactttct ccgccaccga cacgacctgg
     3121 ctcgctgcga tctggtcatc gtctgggagc atgacctcgg ttctatgccg attcccgtgc
     3181 tggagctgaa gggaaacgca ctccatgtaa tgaaacgccg cggacgaccc ccgcaatcga
     3241 cgtctcggtg ggtcaatcga cgggccttct gggcccgtgt atcggcgtct aaggcgtcgg
     3301 ctgtacagag aactcccctc gaccaccccg gggacccaaa ggacccgccc cagtccgatt
     3361 agcggcccag acgcccactt cat
//
LOCUS       DATMBB010000135         2634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_285331, whole genome shotgun sequence.
ACCESSION   DATMBB010000135 DATMBB010000000
VERSION     DATMBB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285331"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VNL92_02855"
     CDS             <1..257
                     /locus_tag="VNL92_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93679.1"
                     /translation="EPEPEPRVTANGLYGVGINGIVVQGASPAASVASTIEEESGLHV
                     QALWLLMRGQWLYFLPASEDIEGGLAEFPDAPVAAFAILT"
     gene            378..1154
                     /locus_tag="VNL92_02860"
     CDS             378..1154
                     /locus_tag="VNL92_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO93680.1"
                     /translation="MPGRFEGKVAIITGAAGGIGKAAAERLASEGARVVAVDLPGSAL
                     DATVAAVQSLGGEAIAVPADVTQSKQVENYVAEARSRFGGVDLFFNNAGIEGWVGPST
                     DYPEDIFDKVLAVNVKGVWLGMKYVVPAMRERGGGAIVNTASVAGLSGTPNLIAYGAS
                     KHAVIGLTKSGALEFARDRIRVNAVCPAPIETRMMRSLERGMNADEPEAVHQMMAAGI
                     PLGRYGEPSEVAALVAFLFSDDASYITGGIYTVDGGSRAR"
     gene            1339..2325
                     /locus_tag="VNL92_02865"
     CDS             1339..2325
                     /locus_tag="VNL92_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWO93681.1"
                     /translation="MSLHEGSLRRWLLRNSAPAIVALVLGFATIPATLVPTYAEETYV
                     IEPGDTLSEIAAKLGMSIASLASLNDIDDHDIIYAGMRLRTSDVDGSADVNPFSLGES
                     AAASESAGGGLIHLVAPGDTLSVIALTYRVSITAIAAANGISNHDVIYVGQSLVIPGD
                     VNPFALDNDVSHESGPSSGSTEELPPPDLTPGPKIGLPSAEAATALDQAAAEFGFEPA
                     LLYGIAFIESGFQTHVVSPAGAIGLMQVLPETGDWAAEELVDGAYDWRTNPVSNARVG
                     AAVLRQLVDLAGGDIRLALAAYYQGWGNIHEYGMLETTERYVEDVLAAASRF"
     gene            complement(2340..>2634)
                     /locus_tag="VNL92_02870"
     CDS             complement(2340..>2634)
                     /locus_tag="VNL92_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93682.1"
                     /translation="GEPVDVFVSGHTHYERLDYREDAVILNSGSPVFPRHKSTRLGTA
                     GLLELGRGTLRAEIVVLGETEGKPNPGVPQVLELREDVVRLETNGYTPPRYLE"
BASE COUNT          443 a          914 c          846 g          431 t
ORIGIN      
        1 cggagccgga gccggagccc cgcgtcacgg cgaacggact ctacggggtc gggatcaacg
       61 gcatcgtcgt ccagggcgcc agccccgccg cgagcgtcgc ttcgaccatc gaggaggaga
      121 gcggactgca cgtgcaggcg ctctggctcc tcatgcgcgg gcagtggctg tacttcctcc
      181 ccgcctccga ggacatcgag ggcggcctcg ccgagttccc cgatgcgccg gtcgctgcat
      241 tcgccatcct gacctgaccg tcccctgcgg cgcgtctcct aaaccgagct actggccgcg
      301 aaccctcccc gcacggtata acgtgggagc gcccgccgtc cggcgtggca gcgaacacag
      361 cgaggaggga gagaagcatg cccggtcggt tcgaggggaa ggtcgcgatc atcaccggag
      421 cagccggcgg catcgggaag gccgctgcgg aacgactcgc ctccgaggga gcgcgggtcg
      481 tcgcggtcga tctaccgggc tcggcgcttg acgcgacggt cgccgccgtg cagtcgttgg
      541 ggggcgaggc catcgccgtt ccggccgatg tcacgcagtc gaaacaggtt gagaactacg
      601 tcgctgaggc gcgctcccgc ttcggtggcg tcgacctctt cttcaacaat gccggcatcg
      661 agggctgggt ggggcccagc accgattacc cggaggacat cttcgacaag gtgctcgccg
      721 tcaacgtcaa aggcgtgtgg ctcggcatga agtacgtggt gcccgcgatg cgtgaacgtg
      781 gcggaggggc catcgtcaat acagcctccg tcgccgggct cagcggcacc ccgaacctca
      841 tcgcctacgg ggccagcaag catgccgtga tcggcctgac gaaaagcggc gcgctcgagt
      901 tcgcgcgcga ccggatccgg gtcaacgcgg tgtgtcccgc ccccatcgag acgcgcatga
      961 tgcgatcgct cgagcgtggg atgaatgccg acgagcctga ggccgtgcat cagatgatgg
     1021 cagccggaat cccgctcggg cgttacggcg agccctcgga agtcgccgcc ctcgtagctt
     1081 ttctcttcag tgacgacgcc tcgtacatca ccggcggcat ctacactgtc gacggtggct
     1141 cacgcgcgcg ctagcgaccg acgctcgagc gtcccgcgcg tacggcggga tgctcgagcc
     1201 ggggagcagc atcgggcccg cacgcgccgt gttcaagtgg attcaaatga ggacccctta
     1261 aggaaacccc cgattgcgtc gataactccg ttagtccctt aatcgccgca aggcgtgaag
     1321 gtaatcggaa ggatcgacat gagtctgcac gaggggagcc tccgtcgctg gctcctcagg
     1381 aattcggctc ccgccatcgt cgcactggtg ctcggcttcg ccaccatccc ggccacactc
     1441 gttcccacgt atgccgaaga gacctatgtc attgagcccg gcgatacgct cagcgagatc
     1501 gcggcgaagc tcgggatgag catcgcctca ctcgccagcc tcaatgacat cgacgaccac
     1561 gacatcatct atgccggaat gcgcctacga acgagtgacg tcgacggctc cgccgacgtg
     1621 aatccgttca gcctcggcga gtccgctgcc gcctccgagt cggccggcgg cggcctgatc
     1681 cacctggtgg cgcccggcga cacgctgagc gtcatcgccc tcacctaccg cgtgtccatc
     1741 accgccatcg cggcagccaa tggcatctcc aaccacgatg tcatctatgt cggccagtcg
     1801 ctcgtcatcc cgggcgacgt gaacccgttc gctctcgata acgatgtctc gcacgaatcc
     1861 gggccctcgt caggctccac cgaggaattg ccgccgcccg acctcacgcc aggaccgaag
     1921 attggactcc ccagcgcgga ggccgccacg gccctcgacc aagccgccgc cgaattcggc
     1981 ttcgagccgg ccctgctcta cggcattgcg ttcatcgaat ccggattcca gacgcacgtc
     2041 gtcagcccag cgggggcgat cggccttatg caggtgctac ccgagaccgg cgattgggct
     2101 gccgaagagc tggtggacgg cgcgtacgac tggcgcacga accccgtctc caacgctcgc
     2161 gtcggcgctg ccgtgctgcg tcagctcgtc gatctggctg gcggcgacat tcgcctggcg
     2221 ctcgcggcct actatcaggg ctgggggaac atccacgagt acggcatgct cgagacgacc
     2281 gaacgttatg tcgaggacgt cttggccgcc gccagccgct tctagcgggt tgtctcccgc
     2341 tactcgaggt atcgcggcgg agtgtacccg ttcgtctcca accgcacgac atcctcgcgg
     2401 agctcgagta cctgcggcac gcccgggttt ggcttcccct ccgtctcacc gagcacgacg
     2461 atctcggccc gcagcgtacc gcggccaagc tccaggaggc ccgccgtgcc gagacgggtg
     2521 ctcttgtggc gcgggaacac cggactgccc gaattgagga tcacggcgtc ctctcggtag
     2581 tcgaggcgct cgtaatgcgt gtgcccggac acgaacacgt cgacgggctc gccc
//
LOCUS       DATMBB010000136         5249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_296028, whole genome shotgun sequence.
ACCESSION   DATMBB010000136 DATMBB010000000
VERSION     DATMBB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296028"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..477
                     /locus_tag="VNL92_02875"
     CDS             94..477
                     /locus_tag="VNL92_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HWO93683.1"
                     /translation="MATTLDGIAVVRSFCEAWAKRDVPAIMAAFADDAVYHNMPLDPV
                     RGKDAIEATINGFMGPAKSVEFRVLNIAASGNVVLTERIDVFDMGDRKIELPVMGTFE
                     LDASGKIVAWRDYFDLGAWTKQTGS"
     gene            618..1793
                     /locus_tag="VNL92_02880"
     CDS             618..1793
                     /locus_tag="VNL92_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93684.1"
                     /translation="MNRFIVGGQTIVLNSLSPDLDRTFVDLYAAFFGGGALELSGFED
                     QVLAFFRQRGDPDPTPDYETHNKYFRNFTPIWRNLLGAGDHDGAERVWAELALGPVLA
                     WEGRSREGSRIHKGTAYYFWGMTALRRGQLDKGYALMHQAVQEDICTFGVDNPNTPAM
                     ALATLDYEKIDQAYRSWVLQQRDFLEERIGDYSSTYSTRDFSLGAFKARFLDKPPKTE
                     VVFLFAYVLARLKELGGLPEHGRQSVFAGQLELTLLFNLALVTEEAIGAKNPNRKNRN
                     RKNRNRGNFLKQACFLLERADKPVKDLRRVNRALDDEKFDRDEKFDRTVRELLDRDYR
                     LDGVTLDPLQSDVALAYVLRNHGAHEVVAPRVVSERFPDIRQSLMNVLFAAVDYLYD"
     gene            complement(1896..2039)
                     /locus_tag="VNL92_02885"
     CDS             complement(1896..2039)
                     /locus_tag="VNL92_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93685.1"
                     /translation="MNVNGGWIAVGIALGVCFGMLFDNLGVGIAIGVAIGVALAAERS
                     PEG"
     gene            2208..2492
                     /locus_tag="VNL92_02890"
     CDS             2208..2492
                     /locus_tag="VNL92_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93686.1"
                     /translation="MDAIGAIVFVVCVVVVSAIVLVSLRIELEVGWTLPAAAFGVALG
                     GFAGSEWFGPWSDWGPTIDGLNILPSLLGMALIGWLAALSTLAPPQELHP"
     gene            2508..3320
                     /locus_tag="VNL92_02895"
     CDS             2508..3320
                     /locus_tag="VNL92_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93687.1"
                     /translation="MRTRTIADALYAPAASATPRRLGVLFSDTRVSWLWLVARVYLGW
                     QWLDAGRHKLEDESWMETGVALRGFWERAVAIPEGGQPAITYGWYRDFLQYMLDHEWY
                     EWFAPLIAIGETALGVALHPGGVYGHRCRDRRLHELQLHARWFREYESSALRARRPAV
                     ARVADGRLPRPRPVAAAVPRHALAAGTHFGDAAQGRGVGGTMLRPALITLHALNSALA
                     VAAGQATVRDRTVIIRWWHWSQGPWWAMFAFVTVLAARLAQDDLRERRASWP"
     gene            3547..4686
                     /locus_tag="VNL92_02900"
     CDS             3547..4686
                     /locus_tag="VNL92_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWO93688.1"
                     /translation="MDPADRDATKVALATQILSKPGSRVLVLRALQIGDLLCAVPALR
                     ALRGRFPEVEISLIGIPWARDFVRRFRAYTDRFLLFPGYPGIPEAPFVPDATLRFFAW
                     AQSQHYDLAIQLHGNGLRTNAFVEMLGAEATSGFFLPGQPAPDSLLFFPYPGAGSEIH
                     RLLSLAEGLGASRSDDLEFPLEAADWAEFEALRKRVGLMEGEYVCLHVGARDPRRRWP
                     AERFAQVADGLASRGLRVVFTGTADEAPMVDAVVEQMSSPSLSLAGQTTLGGLACLID
                     RAALLVAGDTGVSHIAAARGTRSVIIFTFSDPDRWAPLDAQLHRVLVAETARRTSCGD
                     GLARCLMDGCNAADGSEAHPHQVEPEEVLAQADVLLSKGVGLGVA"
     gene            4673..>5249
                     /locus_tag="VNL92_02905"
     CDS             4673..>5249
                     /locus_tag="VNL92_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWO93689.1"
                     /translation="MESPDWQRIERALVVRLDNIGDVVMLTPALRALREAAPRAHITL
                     LASPAGAQVAPMLPWVDDVIVERAVWQDISGTPGCGQERDQALIRRLAEGEFEVAFIF
                     TSFSQSPYPPAYACYLAGIPVRVGQSKEFGGQVLTHWLRSCPDETHQVERNLALLRHV
                     GIVASDGQMELRLGPDVLEAAAGLLARAGLAA"
BASE COUNT          889 a         1608 c         1709 g         1043 t
ORIGIN      
        1 tcctgcgggt ccggccgctg ctgggacggc tgcataaggt ggagactcgc tggaaccacg
       61 agcgcgatgg gcgatagtcc gggaggggga gcaatggcca cgacacttga tggcatagcg
      121 gttgtgcgga gcttctgcga ggcatgggcc aagcgcgatg ttcccgcgat catggccgcg
      181 tttgcggatg acgccgtgta ccacaacatg ccactcgatc cggtgcgggg caaagatgcc
      241 atcgaagcca cgatcaacgg gttcatggga ccggcgaagt cggtggagtt ccgtgtcctg
      301 aacatcgcgg cttcggggaa cgtcgtgctc accgagcgca tcgacgtgtt cgatatgggc
      361 gatcgcaaga tcgagctgcc cgtgatgggg acgttcgagc tcgatgcgtc gggcaagatc
      421 gtcgcctggc gagactactt cgacctcggc gcctggacga agcagacagg gtcctgaggg
      481 cgcagtttgc cgtttgggga cctttcgccg gccgaggtag ggagctcagg tcgaatgccg
      541 atcggttttg cacaccgcct atccgtggcg tcgaacatca cgatcgatgg gcaggcaccg
      601 gacgttgagg gcgcacagtg aaccgcttca ttgttggcgg ccagaccata gtactcaaca
      661 gtctatcccc tgacctcgat cgtacctttg tcgacctgta tgctgccttc ttcggaggcg
      721 gcgcgttgga gctatcgggc ttcgaggatc aggtgctagc cttcttccgt cagcgggggg
      781 acccagatcc aacgccagat tatgagacac ataacaagta tttccggaac tttactccca
      841 tttggcggaa cctcctagga gcaggcgatc acgacggggc agagcgcgtc tgggccgagc
      901 ttgcactcgg accggtcttg gcctgggaag gccgttcgcg cgagggaagc aggattcaca
      961 agggtacggc gtactacttt tgggggatga ctgccttacg acggggccag ctcgacaagg
     1021 gctatgctct catgcaccag gcagtacagg aggatatatg taccttcggc gttgacaatc
     1081 ccaatactcc tgcgatggct cttgctacgc tcgattacga aaagatcgac caagcctatc
     1141 gatcgtgggt tctccagcaa cgggacttcc tcgaggagag gattggcgac tactcgtcga
     1201 catacagcac gagggatttc tctctagggg ccttcaaggc cagattcctc gacaaaccgc
     1261 ccaaaactga ggtagtgttc ctgttcgcct atgtgcttgc gcgcctcaaa gaactgggcg
     1321 gattacccga gcatggccgc caaagcgtct tcgctgggca gctggaacta acactgcttt
     1381 tcaacctggc actcgtcact gaggaagcta tcggggcgaa gaatcccaat cggaagaatc
     1441 gcaatcggaa gaatcgcaat cgggggaatt tcctcaagca ggcttgcttc ctccttgagc
     1501 gcgcggataa accggtaaag gacttgcgtc gcgtgaatag ggcgctggac gacgagaagt
     1561 tcgatagaga cgagaagttc gatagaacgg tgagagagct tctcgatcgg gattatcggc
     1621 tagacggcgt cacgctcgat ccgctccagt cagacgtagc cttggcatac gttctccgga
     1681 atcacggggc gcatgaggtg gtcgcgcccc gggtcgtttc cgagaggttc cctgacatac
     1741 gacagtccct catgaacgtt ctctttgcag cagtcgatta cctgtacgac taggatggtg
     1801 aggcgaagcg ggtcggtcgt cgcgcagagc gccgccggcc agccatcgcc aacgagaggg
     1861 cggctctctg ctcggccctt gcttgatgtg cctcgctatc cctcggggct gcgctcggcg
     1921 gccaaggcca cgcctatcgc aacgcctatc gcgataccaa cgccgaggtt gtcgaacagc
     1981 atgccaaagc agacccccag agcgatgcca accgcaatcc agcctccgtt gacgttcatg
     2041 ggccacctcc gtttgaacgc cggcctctcg ccctctgacg gactgcgtct tcgcagcagg
     2101 gcctcgcgat caggttagcg ctgttgcgag ccgcgtgcac gccggtgtag cgtggcaaga
     2161 aggaagcgcg tccctcggag agggagctgt acgccggagg gtggtgtatg gacgcgattg
     2221 gtgcgatcgt gttcgtcgtg tgcgtggtgg tcgtatctgc catcgtcctc gtgtcccttc
     2281 ggatcgagct cgaagtcggc tggacgctgc ccgcggccgc gttcggggtg gccttgggag
     2341 ggttcgcggg aagcgagtgg ttcggccctt ggagcgattg gggaccgacg atcgatgggc
     2401 tgaacatcct cccatccctg cttggcatgg cgctcatcgg ctggctcgcc gccctttcga
     2461 ccctggcgcc acctcaggaa ctgcatccgt aggcgaggga cgatacgatg aggacacgaa
     2521 cgattgcgga tgcgctctac gctccggcag cgtcggcgac gccgcgccgt ctcggcgttc
     2581 tcttttccga tactcgcgta tcctggctct ggttggtcgc gcgtgtgtat ttggggtggc
     2641 aatggctcga tgcggggcgg cacaaactcg aggacgaatc gtggatggaa acgggcgtcg
     2701 cgttgcgtgg cttctgggag cgggcagttg caattccgga ggggggacag cccgccatca
     2761 cgtacgggtg gtatcgcgac ttcctccaat acatgctcga ccacgagtgg tacgagtggt
     2821 tcgccccgct catcgcgatt ggtgaaacgg cgctcggcgt ggcgcttcat cctgggggcg
     2881 tttacgggca tcgctgccgt gaccggcgcc ttcatgaact tcaacttcat gctcgctggt
     2941 tccgcgagta cgaatcctct gctcttcgcg ctcgccgtcc tgctgttgct cgcgtggcgg
     3001 acggccggct tcctaggcct cgaccggtgg ctgctgccgt tcctcggcac gccctggcag
     3061 ccgggacgca ttttggggat gcggcgcagg gccgaggcgt aggtggcacg atgctgcgcc
     3121 cggcgctcat cacgctgcac gctctcaaca gcgcgttagc ggttgctgca gggcaggcca
     3181 ccgtgcgaga tcggacggtc atcatccgct ggtggcactg gagccagggc ccgtggtggg
     3241 cgatgttcgc cttcgtgacc gtgctcgcag cacgactcgc gcaagacgac ctgcgcgaac
     3301 gtcgcgccag ttggccgtag gccgatagcg cctctcctct cccatcctga ctggcacgaa
     3361 gcgcctttca tcgccgtgcg cccttcgcgg tctccgaatc aatcgacgac gcgtagaaca
     3421 agcgtacggt gctagcgcta gcgctagcac tctgcaacgc ttccggtcct gctcgtgagc
     3481 gtctcgatac tgcttcttct ctacccttgc cacagtactt ctcgttgtgc gaaggagatt
     3541 gcgtgcttgg acccggcaga ccgtgatgca acgaaggtcg cgctggcgac gcagattctg
     3601 agcaagcccg gttctcgagt cctcgtgctc cgtgcgctcc agattggcga cctgctctgc
     3661 gctgtgcccg ccctccgtgc cctccggggg cgattccccg aggtggagat cagcctcatt
     3721 ggcatcccgt gggcgcgcga cttcgtccga cgattccgag cctacaccga tcgcttcttg
     3781 ttgttcccgg ggtaccccgg cattccggag gcgccgtttg tcccggacgc gacgctccga
     3841 ttcttcgcat gggcccagag ccaacactac gatctcgcca ttcagttgca cggcaacggt
     3901 ctgaggacga acgctttcgt ggagatgctg ggcgcggagg cgacgagcgg cttcttcctt
     3961 cctggccagc ctgctcccga ttccctgctc ttcttcccgt acccaggggc aggctccgaa
     4021 atccatcgcc tgctctcgct ggccgaaggt ctcggcgctt caaggagcga cgacctcgag
     4081 tttccgctgg aggccgccga ctgggccgag ttcgaggctc tccgaaagcg agtaggtcta
     4141 atggaaggcg agtacgtgtg cctgcacgtg ggcgctcgcg atcctaggcg gcgctggccc
     4201 gcggagcgct tcgctcaagt cgccgatggg ctcgcttcgc gggggctccg cgtagtcttt
     4261 accggaacgg ccgacgaggc gcccatggtc gacgccgtgg tggagcagat gtcgtcgccg
     4321 tccctctcgc ttgctggcca gacgacccta ggtggcctcg catgcctgat cgaccgggcg
     4381 gctctccttg tcgcgggaga taccggcgtc tcgcatatcg ccgcggcgcg agggacccgc
     4441 agcgtcatca tcttcacctt ctcggaccca gatcgatggg ctccgctcga cgcgcaactc
     4501 catcgggtgc tcgtcgccga aactgcacgc cgcacgtctt gcggcgacgg gctagcgcgg
     4561 tgcctcatgg acggctgcaa cgccgctgat gggagtgagg cgcacccgca ccaagtcgag
     4621 ccagaggagg tactcgcgca ggcggatgtt ctgctaagca agggggtcgg ccttggagtc
     4681 gcctgattgg cagcgaatcg agcgagcgct cgtcgtgcgc ctcgacaaca tcggtgacgt
     4741 cgtgatgctc acgccggcgt tgcgagcttt gcgtgaggca gcaccgcgag cgcacatcac
     4801 gttgctcgcc tcgccggcgg gggcgcaagt tgccccgatg ttgccctggg tcgacgacgt
     4861 gatagtggaa cgagcggtat ggcaggacat ctccggtacc ccgggctgcg gccaggaacg
     4921 cgatcaggcg ctcatccgcc gcctggcgga gggcgagttc gaggtcgcat tcattttcac
     4981 cagcttcagt cagtcccctt acccgccggc ctacgcctgc tatttggcag ggatacccgt
     5041 gcgagtaggc cagtcgaagg aattcggtgg gcaagttctt acccactggc tccgatcctg
     5101 tcccgacgag acgcatcagg tcgaacgcaa tctggcgctc ctacgccacg ttggcatcgt
     5161 cgcgagcgat ggtcagatgg agcttcggct gggccccgat gtgctcgaag cggcggcggg
     5221 gctgctcgcc cgggccggac tagcggcgg
//
LOCUS       DATMBB010000137         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_285006, whole genome shotgun sequence.
ACCESSION   DATMBB010000137 DATMBB010000000
VERSION     DATMBB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285006"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1916
                     /locus_tag="VNL92_02910"
     CDS             177..1916
                     /locus_tag="VNL92_02910"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWO93690.1"
                     /translation="MTAPAPLRVVLGQINMVVGDIEGNARRILQTVERARELEADVVA
                     LHELAVTGYPPEDLLLRRAFIDENLAATREIARACTGLFAIIGFVDARDDIYNAAAVV
                     HDGAIVDVYHKRYLPNYGVFDENRYFQAGDRNPVYEMNGVTIGISICEDIWYPGDPIR
                     AQALAGAEVVVNINGSPYHMRKVTGRERMLATRAADHTVSICYVNTVGGQDELVFDGG
                     SMVLNERGERIAMAKQFEEDLLACDVDVQAVRQYRLHDPRRRKERAEIRPEDIAERII
                     IDGHASRSERSGLPPYDVPPLGEDAEAYHALVLGTRDYVRKCGFQDVLVGLSGGIDSS
                     LVATIASDALGPSHVYGVSMPSRYSSEGSITDAKELAENLGIHFTIIPIEDTFVAMLE
                     TLAPTFANTVPGLAEENLQARIRGNLIMAISNKFNRLVLTTGNKSEMATGYATIYGDM
                     AGGFAVLKDVPKTLVYRLAEWRNAQGDTAPIPQNVLTKAPSAELRPGQTDQDSLPPYA
                     VLDPILQAYVEDDRSLEEIVRMGFDEATVRRVIRLVTVSEYKRRQAPPGVKITPRAFG
                     RDRRLPIANAYGG"
     gene            1975..>2456
                     /locus_tag="VNL92_02915"
     CDS             1975..>2456
                     /locus_tag="VNL92_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWO93691.1"
                     /translation="MKVLVTGGAGFIGSHVTVKLLDAGHDVIVYDDLSRGFRELVDDR
                     ADFVLASLHDQLRLREALRGTDAVVHMAGFIIVPESVEHPEMYYQNNVEGSRSLLEAM
                     EATGVRRIVFSSSATVYGEPDAVPLTEDAPIKEAANPYGQTKIDVEALLHEAHARSGL
                     "
BASE COUNT          436 a          855 c          751 g          414 t
ORIGIN      
        1 ggcggcggcg ctcgctgtca tgggcggcgc cgcggtctgg tcggcgaggc gacgcgccct
       61 ctagtccccg atcacgtcga gtacaggagc ggggtccttc ttgggaagga ccccgctcat
      121 tctcttcctt gaccactccc gattcgcggc acgataattc gggcacggag gtcaccttga
      181 ccgcacccgc cccgctccgc gtcgttctcg gccagatcaa catggtcgtg ggcgacatcg
      241 agggcaacgc gcgccgcatt ctgcagacgg tcgagcgagc gcgtgagctc gaagccgacg
      301 tcgtcgccct ccacgagctc gctgtcaccg gatatccgcc cgaggacctc ctcctccgcc
      361 gcgccttcat cgacgagaac ctcgccgcca cgcgcgagat cgctcgcgct tgcaccggat
      421 tgttcgccat catcggcttc gtcgatgcca gggacgacat ctacaacgca gccgctgtcg
      481 tccacgacgg cgctatcgtc gacgtgtacc acaagcgcta tttgcccaac tacggcgtct
      541 tcgatgagaa ccgctacttc caggctggcg accgtaatcc cgtgtacgag atgaacggcg
      601 tcacgatcgg gatcagcatc tgcgaggaca tctggtatcc cggcgacccc attcgggcgc
      661 aggccctggc gggcgctgag gtcgtcgtca acatcaacgg gtcgccctat cacatgcgca
      721 aggtcacggg acgagagcgc atgctcgcta cacgcgccgc cgatcacacg gtgagtatct
      781 gttatgtcaa tactgtcggc gggcaggacg agctcgtctt cgacggcgga agcatggtcc
      841 tcaacgagcg cggcgagcgc atcgcgatgg ccaagcagtt cgaggaggac ctcctcgcct
      901 gcgacgtcga tgtgcaggcg gtccgccagt atcgcttgca cgatccgcgt agacgaaagg
      961 agcgagcgga gatccgcccc gaggacatcg ccgaacgcat catcatcgat ggccacgcct
     1021 cccgctcgga gcgctcgggc ctgccgccgt atgacgtgcc gcccctcggg gaagacgcgg
     1081 aggcttacca tgcccttgtc ctaggcacgc gcgactacgt gcggaagtgc ggctttcagg
     1141 atgttctcgt cggcctttcc ggcggcatcg attcgagcct tgtggcgacc atcgcctccg
     1201 acgccctagg accctctcat gtgtacggtg tgtccatgcc ctcgcgctat tcttccgagg
     1261 ggagcatcac ggatgcgaag gaacttgccg agaacctcgg catccacttc accatcatcc
     1321 ccatcgaaga cacgttcgtc gccatgctcg agacgctcgc acccacgttc gcgaacacgg
     1381 tccccgggct ggccgaagag aacctgcagg cgcggatccg cggcaatctc atcatggcga
     1441 tctccaacaa gttcaaccgc ctcgttctca ccaccggcaa caagagtgag atggcgacgg
     1501 gctacgcgac catctacggc gacatggcgg gaggcttcgc cgtcctcaag gacgtgccca
     1561 agacgctggt gtatcgactc gcggagtggc gcaacgccca aggcgacacg gcccccatcc
     1621 cccagaacgt gttgacgaag gcgccgtccg cggagctccg ccctggccag acggatcagg
     1681 atagcctgcc cccctacgcc gtgctcgatc ccattctgca ggcctacgtc gaggacgacc
     1741 gcagtctgga ggaaatcgtt cgtatggggt tcgacgaggc gacggtgcgc cgcgtcatcc
     1801 ggctcgtcac ggtgagcgag tacaagcgac gccaagcgcc gccgggcgtg aagatcacgc
     1861 cgcgcgcgtt cggtcgcgac cggcgtctgc cgatcgcgaa cgcctacgga ggctgatgtc
     1921 ggagcatcga accccccgta gccccggcga caaccgcgcc tcgatccccg ccccatgaag
     1981 gtgctggtca ccggcggcgc tggattcatc ggctcccatg tcacggtgaa gctgctcgac
     2041 gccggccatg atgtcatcgt ctacgacgat ctgagccgcg gcttccgcga gctcgtcgac
     2101 gaccgcgcgg acttcgttct cgcctcgctg cacgaccagt tgcgcctccg cgaggccctt
     2161 cgcggcacgg acgccgtcgt tcacatggcc ggcttcatca tcgtgcccga gagcgtcgaa
     2221 catcccgaga tgtactatca gaacaacgtg gagggctccc gaagcctgct cgaggcgatg
     2281 gaggcgaccg gcgtgcggcg catcgtcttc tcgtcatcgg cgaccgtcta cggcgaaccc
     2341 gatgccgtcc ccctcaccga ggacgcgccg atcaaggaag ctgccaaccc ctacggccag
     2401 acgaagatcg acgtcgaggc cttgctccac gaggcccacg cacgctcggg tttgga
//
LOCUS       DATMBB010000138         3550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_364539, whole genome shotgun sequence.
ACCESSION   DATMBB010000138 DATMBB010000000
VERSION     DATMBB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364539"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VNL92_02920"
     CDS             <1..701
                     /locus_tag="VNL92_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93692.1"
                     /translation="VFEGGYWTPDFAKGLGSVAFRDEPEGGQSFVPQPDRVSHWWNGT
                     SPRPSVPPDAVAVHVRVQVRLIPNSDPLADLSAARYVANQSADSYRSPSGGWLGDIAM
                     PRLKLITPQWQWFTMTTLYEAELRANPPPPLLPPASMPDDGDGQSPPTGVPGLTVTPA
                     SPYRAGLNSITVVGGGAPSAIADVVATESRRAVIALWLLQDNAWLYFLPQAPERSTLH
                     SVSPAAAALFVELA"
     gene            991..1941
                     /locus_tag="VNL92_02925"
     CDS             991..1941
                     /locus_tag="VNL92_02925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWO93693.1"
                     /translation="MNGRSVPVSVALPVYNGEQFLAEAIESVLAQTFGDFELLICDNA
                     STDATPHICQMYASRDRRVRYYGSDKNRGASWNFNRAFGLASGQYFKWIAADDYLAPT
                     FLERCLMALNDRPEAVLAYPNAVCVDESGGFLHDYNEIMDQGLWHERAVYRFRRLLNE
                     FERNGGTSANAYVFGLIRTSALRRTDLLGNYIAADLGLVAHLVLLGPFVEVPEHLSFI
                     RAHEGSSSWFRAAGWSLDRAQRFFDPTVRGRLALAISVRRRHYEYCRAAFRTPLPIDE
                     RARVVLSCAEAATKRLERRLAPATARERYRADVDSGGRRG"
     gene            complement(1902..2699)
                     /locus_tag="VNL92_02930"
     CDS             complement(1902..2699)
                     /locus_tag="VNL92_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWO93694.1"
                     /translation="MYEQTKVLVVGTDRTEANRVADWLAQTQYSTITAGHWDQGLRQL
                     YTARPDALILLNAPGSPVQWKDVDVVRSLCDIPVFVVADRANRASLEKALDLGLAGYL
                     VRPLQFRSLTERLTRVLQKTPTNRLVPSGVFQYENLTIDWRRFEVRVDGHPVRLSTKE
                     FALLSLLVERQGEVVMYGEILSKVWGQNYDLMERRNVKLYIWYLRRKLERDPTRPRWI
                     LTKNGLGYMFASEPDDSCDAADAEGCGRHAPARRRYPRRPPESTSAR"
     gene            complement(2935..>3550)
                     /locus_tag="VNL92_02935"
     CDS             complement(2935..>3550)
                     /locus_tag="VNL92_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93695.1"
                     /translation="RAYPGPFRLDASPDLPQAAREGLAALPVLGDLMAASPDTTGRRS
                     IERLFESLESDLKSIGGDLEHAYRSQPRCVIHGSFSPGVLLFEGDNLVSVDGMQSARY
                     DCLLADLAQAITAFCSTKKRGLFSPPALDPDRYRTFLRAYRKEAPLTREQLDVFALVL
                     RIQRLLGVMEESRRFLTATLHGQDSIHLAPTSRNGACSATDAAL"
BASE COUNT          646 a         1147 c         1078 g          679 t
ORIGIN      
        1 acgtcttcga gggcggatac tggacgccgg atttcgcgaa ggggctgggc tccgtcgcgt
       61 tccgcgatga gccggagggc ggtcagtcct tcgtgccgca gcccgaccga gtctcccatt
      121 ggtggaacgg gacttcgcca cgaccaagcg taccgcccga tgccgtggcg gtccatgttc
      181 gtgtgcaggt gcggctcata cccaactccg accctctggc cgatctcagt gcggcccgct
      241 atgtcgccaa ccagagcgcc gactcttatc gctctcctag cgggggttgg ctaggtgaca
      301 tcgcgatgcc ccggctcaag ctgataacac cgcagtggca atggttcacc atgacaacac
      361 tctacgaagc agagctgcgg gcaaaccctc caccgccttt gctcccgccc gcatcgatgc
      421 ccgacgacgg agacggccag tcgccaccga ccggcgtgcc cggcctgacc gttacacctg
      481 cctcgccgta cagggccggt ttgaactcga taacggtggt cggtggaggt gcacccagcg
      541 ccatcgcgga cgtcgtggcg accgagagtc gccgtgccgt tatcgcactg tggttgctcc
      601 aagacaacgc ctggctctac ttcctgccgc aggccccaga aagaagtacg ctgcactccg
      661 tctctcctgc cgcggccgcc ctcttcgtgg agctggctta ggcccgcctc gcggatcttg
      721 cgaccaatcg tacgaagtcc gtcatgagtc ggtctgaatt tggttcctcc cgactcgtca
      781 gattgcaggg cagagcccgc cgacgttgac gggccatccg cggctgccat accttcctcc
      841 acaagcgtgg catcgaaagc ggatgcccta ccctgcgaag catgcgtacc gacctggcgc
      901 gtcaggatat gctcgtcctg cgccagacct gaagcctctt ctgcaattgg cgagcacgtc
      961 tcctacctcg ccaccattgt cgggagcgaa atgaacggtc ggagcgtacc cgtatccgtc
     1021 gccctgcccg tctacaatgg cgagcagttt ctggcggagg cgatcgagtc ggtcctggcc
     1081 cagacgttcg gcgacttcga gcttctcatc tgcgacaacg catcgaccga cgcaacgccg
     1141 catatctgcc agatgtacgc gtcccgcgac cgacgggtcc gctattacgg gagcgacaag
     1201 aataggggag cgtcgtggaa cttcaatcgt gccttcgggc ttgcgtccgg tcagtacttc
     1261 aaatggatcg cagccgatga ctatcttgcc ccaacattcc tcgaacgttg cctcatggcc
     1321 ctcaacgacc gcccggaagc cgtgctcgct taccccaatg ccgtctgcgt cgacgagtcc
     1381 ggcggtttcc ttcacgacta taacgagatc atggaccaag gattatggca cgagcgggcc
     1441 gtgtaccgct tccgacgcct tctcaacgag ttcgaacgga atggcgggac atcggcaaac
     1501 gcctacgtct ttggcttgat cagaacgagc gccctgagga ggaccgacct gttagggaac
     1561 tacatcgccg ccgacctcgg actcgttgcc cacctcgtcc tcctcggccc gtttgtcgag
     1621 gtacccgagc atctatcatt catccgggct cacgagggct cgtcgtcctg gttccgcgcc
     1681 gcgggttgga gcctcgaccg cgctcaacgg ttcttcgatc ccacggtccg cggacggctc
     1741 gcgctggcga tctcggtcag acggcgccac tacgagtact gccgggcggc atttcgtacg
     1801 ccactaccga tcgacgagag ggcacgcgtg gtcttgtcgt gtgcggaggc agccacgaag
     1861 cgactcgaga gaaggctcgc gccggccacg gcccgcgaac gctatcgtgc tgatgtggac
     1921 tcgggtgggc gccgagggta gcgacgtcga gccggagcgt gccttccgca tccttcggca
     1981 tccgcggcat cgcaggaatc gtcgggttcg gacgcgaaca tgtaccctag cccgttcttc
     2041 gtcaggatcc agcgcggccg ggtcgggtct cgctccagct tgcgtcgcag gtaccagatg
     2101 tagagcttga cgttcctacg ctccatcaag tcgtagttct ggccccacac cttcgagagt
     2161 atctccccat acatgactac ctcaccctga cgctcgacaa gcagggacag gagcgcgaat
     2221 tccttcgtgc tgagtcgcac cgggtgacca tcgacccgaa cctcgaatcg ccgccagtcg
     2281 atggtgaggt tctcgtattg gaatactccg ctaggtacca accggttagt cggtgtcttc
     2341 tgcaggaccc gcgttagccg ctcggtgagc gatcggaatt gaagcggccg aacgagatat
     2401 ccggccagtc ctagatccag cgccttttcg agagaggccc ggttggcacg atcggccacg
     2461 acgaacaccg ggatatcgca caagctgcgg acgacatcca cgtctttcca ctgaacggga
     2521 ctgccgggcg cgttcagcag gatgagggca tccggtcgcg cggtgtagag ctgtcgtagc
     2581 ccttggtccc aatggccggc cgtgatggtc gagtactgtg tctgggccag ccagtctgcg
     2641 acccggttgg cctccgttcg gtcggtaccg accaccaaga ccttcgtctg ttcgtacatg
     2701 ccgtcctccc tgcgggggca cagtattccc gattagatgc cccaggttga gcaaaacagc
     2761 gccatcgccc cggtcaacgc aacgcgtcgc gcccgtcctg ccggcgctgt gacgcgcggc
     2821 ggccctctcc tgagagggtt ggggcgatcc tcgatattgc tcgcgtgaca acaatcaatt
     2881 cgatccagcc tgcctctgta ggggctcgtc cgcgacgccg atagatttgc attgtcatag
     2941 agccgcgtcc gtagcggagc aggccccatt cctcgacgta ggagcaagat gaatcgagtc
     3001 ctgcccatgt agggtcgcgg tgaggaaccg tctgctctcc tccatgactc ccaggagccg
     3061 ttggatgcgc aggacgagag caaacacatc gagctgctct cgagtcaaag gcgcttcttt
     3121 ccgatatgcg cggaggaacg tgcggtagcg gtcgggatcg agggcgggcg gcgagaagag
     3181 tccccgcttc ttcgtggagc aaaacgctgt gatggcctga gcgaggtccg ctagaaggca
     3241 gtcgtacctc gccgattgca tgccatctac agaaacgaga ttgtcgcctt caaacaacag
     3301 gactcccggg ctgaaggagc catggatgac gcacctcggt tgagaccggt aggcatgttc
     3361 aaggtcgccg ccaatactct tgaggtcact ctcaagtgac tcaaataggc gctcaatgct
     3421 tcggcgcccc gtcgtgtcgg gcgatgcggc catgagatca cctagcaccg ggagagcggc
     3481 gagtccctcc ctggcggcct gtggaagatc gggcgaagcg tcgaggcgga atggcccagg
     3541 gtaggcccgc
//
LOCUS       DATMBB010000139         8367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_471023, whole genome shotgun sequence.
ACCESSION   DATMBB010000139 DATMBB010000000
VERSION     DATMBB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471023"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VNL92_02940"
     CDS             <1..269
                     /locus_tag="VNL92_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HWO93696.1"
                     /translation="VDYQILQGQEAVVVASGQRAEDMALRLKYAGLSGLVVEPLVPAA
                     LRRALAAGPAERPLFVLPTYTAMLQAREVLGDWAAAGHFWEAGD"
     gene            266..1036
                     /locus_tag="VNL92_02945"
     CDS             266..1036
                     /locus_tag="VNL92_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HWO93697.1"
                     /translation="MSQPCIRIAYLYPTLMNIYGDRGNIICLRRRCQERGIEVDVTEL
                     GIGDPFDAACYDLIVIGGGQDREQRRVAPDLVAKADAIREAVDSDAPLLAVCGGYQLM
                     GREYRTADGDTLSGAGIFDVTTVHPGPGARRCIGNVVARSEELHAPLVGFENHGGRTY
                     LGPNARPLARVKEGLGNNGEDGTEGTVYRRAIGTYLHGSVLPKNPALADRLIADALRH
                     RYGDDAPALEPLDDRLELAAQTMAQRLRKRGRSRRPTA"
     gene            1199..1831
                     /locus_tag="VNL92_02950"
     CDS             1199..1831
                     /locus_tag="VNL92_02950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWO93698.1"
                     /translation="MEIVLGQGSPVATVEPTPVRSDARLEALAGFTAPIEGGCLPSSD
                     LVMPNASREYRNGVHEGVDFYDGDSCVPVGRGTDVVAALDGVVVRADWDYRDLTSAEA
                     AVQDSTEESLDRFRGRQVWIDHGMLAETGERVITRYAHLDSIEEGIDVGDEVSAGQII
                     AHVGNSGTPEGVTDPNAELHLHFEVRVGDRFLGEGEPPDVVREAYERLFS"
     gene            1868..2500
                     /locus_tag="VNL92_02955"
     CDS             1868..2500
                     /locus_tag="VNL92_02955"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWO93699.1"
                     /translation="MSRDIEGPEEHEEAVDERPQQRREWSGPMRSIVLPLAAVAAIVG
                     VIWAIEQRNDGAEVAGDTGIVESEPGAEADEGKPAPDFVLEAIEGGSVRLSDYGGRPV
                     FVNFWATWCKPCRQEIPEMVEAFDRYAGEGLVIIAINVQESRDAAQGFVDEFGMDFPV
                     GLDVRGDVAEAYRVLGLPTSFFIDRNGVIQAIHRGPLNADQMDDRLQEIL"
     gene            2519..4243
                     /locus_tag="VNL92_02960"
     CDS             2519..4243
                     /locus_tag="VNL92_02960"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWO93700.1"
                     /translation="MAKATTPASLRGGAGAHFWRDPFRSLWRLFTSVRFAFALTGFLA
                     FAGLLGVLIPQIPLAMRDNAPAIDAWLLEQGGKFGALTEPMYRVGLFNVFGSWWFRIL
                     LGVLVVSVTVCTANRMPPTFRNVFRPRERVPDSYFERARSRAGAANVAGVDALVQTFR
                     RRRYRVKRFDEQDGVTYLFADRFAWAQLATFVSHLGLIVVLAGAVVSLFLTKESRVFL
                     GEGTTAAIGNLEDGLLQVKVLDAVERFDDEGHIIEYRTDMAVYKQGEEVCRGASTVNS
                     PLSCEGYRFHQSTFFPDGAALSVADEASGRTLYSETLPLLDPWPYPRVVVRDATNGEV
                     LFDESVPLTASLEGAFLSIIRVPERDLTLWVGARPEEEDSWSLVVFQVDSDIAESRVG
                     TVPPGGDEPIRLIVPEGGTARAADLDFAFPAISGAASSIVQDLPDQRDVLLQMIPDDG
                     DGRRLVVSGLGDSAFELGEGEWTVVDGKRYTFEGQREFAGLTVRRDPGTPIIWIAGGM
                     ILLGLVGTFYLPRRRLWAKVTTDRVMLAGVAERTVDFKNELRKLLRDAGNKLDAKSEN
                     GDDDDDED"
     gene            4268..5152
                     /locus_tag="VNL92_02965"
     CDS             4268..5152
                     /locus_tag="VNL92_02965"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWO93701.1"
                     /translation="MTALALLLPLLKIEIGIDPEIGEIAGFLITWHGVFTAVGIGAGL
                     WLALWLADKRAIDADEAYTVGLLVVIGGIIGARALYVIENWSDFEDDLGEIIAITEGG
                     ISIYGALLGGTIFGLVYAWLRPGKIPILAGGDCAAAGALIGQAIGRIGDVINGEHIAE
                     SSDLAWAVEYTHIDSPSFGLAAQHPAVAYELLGDLLILGVVLVLFFRVRRPGWGFFTW
                     FFGYGLLRFLVSFLRQDEEVLGDLVMAQAIAIGGMAIGLAGYAWLAIRGEPAGPSRAE
                     RRRARREAEQRERASPAR"
     gene            5309..6811
                     /gene="nusA"
                     /locus_tag="VNL92_02970"
     CDS             5309..6811
                     /gene="nusA"
                     /locus_tag="VNL92_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWO93702.1"
                     /translation="MKNDFLVAISQLSAEKNLPREVVFEAVETALASAYKKEEVHANN
                     VTAKIDPATGDVRVWAAKEVVEIEEDVADDRDQIPLAEARKFDPNVKIGDTLVYEVTP
                     ANAGRIAAQTAKQVVMQRLREAEREVVFEEFAQREGDIVSGVITRVESRQVLVDLGKT
                     EAVMPPPEQVRSEHYRPGQRMKFYVLQVFRANKGPQVIVSRTHKDLVKRVFELEVPEI
                     FKGVVEIKAIAREPGSRTKVAVYSRQEGIDAVGACVGLRGVRIQNVVNELQGERIDVI
                     QWDPEPRRFVANALSPAQVLAVRIYEPTNTAYVAVPDRQLSLAIGKEGQNARLAAKLT
                     GRRIDIKSQTAAEEEYGGLEPPLPEEPAVAVASISEPAIEAGVTAEPHADVEVAEVED
                     EFALPPEEEAALAFEEPVPVEVPPTEQEPVVAAERGKPALRFAEDILGAPERAGDRRR
                     RREFDEPEVPAKPVKKKKAAKARRLLPDEEEEVLDELDDVEDMEYPGLRR"
     gene            6848..7150
                     /locus_tag="VNL92_02975"
     CDS             6848..7150
                     /locus_tag="VNL92_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HWO93703.1"
                     /translation="MSGKARRIPQRTCVGCREATAKRQLRRVVRTPEGGVAVDPSGRA
                     NGRGAYVHDSAECWRDALQRDALGRALRTTISPEDRASLEAYAGALPSEAPSPALP"
     gene            7218..>8367
                     /gene="infB"
                     /locus_tag="VNL92_02980"
     CDS             7218..>8367
                     /gene="infB"
                     /locus_tag="VNL92_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWO93704.1"
                     /translation="MATGTRPQTTPDPGDRKVALPRALTVAELADILQVSAIDVIKEL
                     MKNGVMARINQVIDFETAAIVASGFGFEPEEERQHVEEAAEAVEARTSRRVVDESATH
                     PRPPVIAVLGHVDHGKTSLLDRIRSTDVTSGEAGGITQHIGAYQVEVGDRVLTFLDTP
                     GHEAFTQMRARGAQVTDVAVLVVAADDGVMPQTVEAINHVRAAGVPMVVALNKIDVPN
                     ANPDRAKQQLMEHGVVVEEYGGDTVCVPVSARTGDGIDDLIESLILVSDLFELKANPD
                     RPAVGVVIEAKLDLQRGPVATILVQTGTLHVGDSVIVGETWGKLKAMFDDKGQRVKSA
                     GPSKPVEIMGLQDVPIAGDQLTVVDSDRDARAQVETMRREKAAEALKER"
BASE COUNT         1494 a         2498 c         2875 g         1500 t
ORIGIN      
        1 atgtggacta tcagattctg cagggccagg aagccgttgt cgtcgcttcg ggccagcggg
       61 cggaagacat ggccctccgg ctgaaatacg cgggcctgtc cgggctcgtt gtggagccct
      121 tggttccggc tgccctgcgc cgtgcgctgg cagccggacc cgccgaacgg ccgctcttcg
      181 tgctgccgac gtacacggcg atgctgcagg cccgcgaggt cctcggcgat tgggccgcgg
      241 cgggccactt ttgggaggcg ggcgattgag ccagccgtgc attcgcatcg cgtatctcta
      301 tcccaccctc atgaacatct acggcgatcg aggcaatatc atctgccttc gccgtcgctg
      361 ccaggagcgg ggcatcgaag tggatgtgac ggagcttggc atcggcgatc ccttcgatgc
      421 tgcctgctac gatctcatcg ttatcggtgg cggccaggac cgggagcagc gacgagtcgc
      481 ccccgacctc gtcgcaaagg ccgacgcgat ccgcgaggcc gtagattcag atgcgccgct
      541 tctcgcggta tgcggtggct atcagctcat ggggcgcgaa tatcggaccg ccgatggtga
      601 tacgctgtcg ggtgccggca ttttcgacgt tacgaccgtc caccccgggc ccggcgctcg
      661 gaggtgcatc ggaaacgtcg tcgctcgcag cgaagagctc cacgctccgc tggtcggctt
      721 cgagaatcac ggcgggcgca catacttggg cccgaacgcc cgtccgctcg caagggtgaa
      781 ggagggcctc ggaaacaacg gtgaggacgg cacggagggc acggtgtatc ggcgggccat
      841 cggcacgtac cttcatggat cggtgttgcc caagaacccg gctctcgccg atcgtctcat
      901 agcggacgcg cttcgccacc gctatggaga cgacgcgccg gcgctcgagc cgctcgacga
      961 tcggctggag ctggcggcgc agacgatggc acaacgcctg cgcaaacgag gccgctcgcg
     1021 ccggccaacg gcgtagaggc gggcagccct cgtcacccgc ctgaatcgca ggagaccaga
     1081 cattcaaact cgaaacaatc cgctgagatt ctggcggtgg gccgcactcg gtgccggcat
     1141 acttctgtta tcggcgttga ttcgtgcgca ggttggcggc ggggaggatg attcggacgt
     1201 ggagatcgtc cttggccagg gttcgcctgt agcgaccgtc gagcccacgc ccgttcgctc
     1261 cgacgcgcgc ctcgaggcac tggcgggctt cacggctccc attgaaggcg ggtgtctgcc
     1321 ctcgtcggac ctcgtgatgc ctaatgcgtc tcgtgagtac cggaacggtg tgcacgaagg
     1381 ggtcgacttc tatgacggcg attcctgcgt ccccgtcggg cgtggcactg atgtcgtggc
     1441 ggcgctggat ggcgtcgtgg tgcgggccga ctgggactat cgtgacctca cctcggccga
     1501 agccgcggtc caggattcga cggaggagtc gctcgaccgc ttccgtggtc gccaagtgtg
     1561 gatcgaccac gggatgctgg cggaaaccgg cgagcgtgtc atcacgcgct atgctcatct
     1621 ggacagcatc gaagagggca tcgacgttgg ggacgaggtg agtgcgggcc agatcattgc
     1681 ccatgtcggg aacagcggta cgcccgaggg cgtgaccgac ccgaacgccg agcttcacct
     1741 tcacttcgag gtgcgcgttg gggatcgctt cctcggtgag ggcgaaccgc ctgatgtcgt
     1801 acgggaagcg tatgagcggt tattctcgta acatcgctga accgagatcg cctgggcgag
     1861 gagttgcatg agcagggata tcgaagggcc tgaggagcac gaggaggcag tcgacgagag
     1921 gccgcagcag cggcgcgagt ggtcggggcc tatgcggagc atcgtgctcc cgctcgccgc
     1981 ggtcgcagcg atcgtcggcg tcatctgggc tatcgagcag cgtaacgacg gcgcggaggt
     2041 cgccggcgat acgggcatcg tcgaaagcga gccgggggcg gaagccgacg agggcaagcc
     2101 cgcaccggac ttcgtgctcg aggcgatcga gggcggcagc gtgcgcttga gcgactatgg
     2161 ggggcgaccc gtattcgtga acttttgggc gacatggtgc aagccttgtc gccaggagat
     2221 cccggagatg gtcgaagcgt tcgatcggta cgcgggcgag ggactcgtga tcatcgcgat
     2281 taatgtccag gagagccgcg acgccgcgca gggctttgtg gacgaattcg gcatggattt
     2341 cccggttggc cttgatgttc gaggagacgt tgctgaggcc taccgagtcc tcggcctgcc
     2401 gacgagcttc ttcatcgacc gcaacggtgt catacaggcc atccatcgag gccctttgaa
     2461 cgccgaccag atggacgatc gtttgcagga gattctctag ccgccgggag gcggaatcat
     2521 ggcgaaggca acgacgcccg cttctctacg cggcggagcg ggggcacact tctggcgaga
     2581 tcctttccgc tcgctttggc gcctgttcac ctcagttcgc ttcgccttcg ccctgacggg
     2641 attcctcgcc tttgccggcc tgctcggcgt cctcatccca cagatccccc tggccatgcg
     2701 tgataacgcc ccggcgatcg atgcgtggct cctcgagcaa ggtggaaagt tcggtgctct
     2761 gactgagccc atgtatcggg tcgggctatt caacgtcttc ggctcgtggt ggttccggat
     2821 tctgctcggg gttctcgtgg tgtcggtgac ggtatgcacg gcgaaccgga tgccgcccac
     2881 gtttcgcaac gtattccgcc ctcgagagcg cgtgcccgac tcgtacttcg agcgcgctcg
     2941 ttcccgggcg ggggccgcga acgtcgccgg tgtcgatgcg ctggtccaga cctttcggcg
     3001 ccgccgctat cgcgtgaagc gcttcgacga gcaggacgga gtcacgtatc tctttgccga
     3061 tcgctttgcc tgggcccaac tcgcgacctt tgtcagccat cttggcctca tcgtcgtcct
     3121 cgccggcgcc gtggtctccc tgtttctcac gaaggagtcg cgcgtcttcc ttggggaagg
     3181 gacaaccgca gcgataggga acttggagga tggccttctc caggtcaaag tcctcgatgc
     3241 ggtggagcgc ttcgacgatg agggccacat catcgaatat cgaaccgaca tggcggtgta
     3301 caagcaaggg gaggaggtgt gcaggggcgc atcgacggtg aattcgccgc tcagctgcga
     3361 aggctaccgc ttccaccaga gcaccttctt ccctgatgga gcggcgctgt ccgtcgccga
     3421 cgaggcaagt ggccgaaccc tatacagcga gacgctgccg ctactcgatc cctggcccta
     3481 tccacgcgtc gtcgtccgcg atgcgacgaa tggcgaggtg ctgttcgacg aatcggtgcc
     3541 gctcacggct tcgctggagg gagcatttct ttccatcatc cgcgttccgg agcgcgacct
     3601 gacgttgtgg gtgggcgcac ggccggagga ggaagattcg tggtcacttg tggtcttcca
     3661 ggtcgactcc gacatcgcag aatcacgtgt gggcaccgtt cctcccgggg gcgatgagcc
     3721 gatacgcctc atcgtgccgg agggaggaac ggcgagggcc gccgacctgg acttcgcatt
     3781 tcccgccatc tccggcgccg cttcgagcat tgtgcaggac ctgcccgacc agagggacgt
     3841 gttgctgcaa atgattccag acgacgggga tgggcgacga cttgtggtgt cgggcttggg
     3901 cgacagcgcc ttcgagctcg gtgagggcga gtggacggtt gtggacggca agcgatacac
     3961 cttcgagggg cagcgcgagt ttgccggact cacggtgcgt cgcgatccag gcacgcccat
     4021 catttggata gccggcggca tgatcttgct tggcctggtc ggcacgttct atctgccacg
     4081 acggcgcctc tgggccaagg tgacgacaga ccgcgtgatg ctcgccggcg tggcggaacg
     4141 caccgtcgac tttaagaacg agctgcgaaa gctgttgcgg gacgccggca acaaactgga
     4201 cgcaaagagc gagaacggcg acgatgacga cgacgaggat tgacgaacca cgattggaga
     4261 ggcggccttg accgcacttg ccctcctgct cccgcttctg aagatcgaga tcggcatcga
     4321 tcctgagatc ggcgagatcg ccggctttct catcacgtgg cacggagtgt tcacggccgt
     4381 gggcatcggc gccgggctct ggttggcact atggctcgcg gataagcgcg ccatcgatgc
     4441 cgatgaggcg tacaccgtag ggcttcttgt ggtcattggc ggaatcatcg gagcacgtgc
     4501 cctgtacgtc atcgagaact ggagcgattt cgaggacgac ctcggagaga tcatcgccat
     4561 cacggagggt ggcatatcga tctacggagc cctgcttggg ggcaccatct tcggtctcgt
     4621 ctatgcttgg ctgcggccgg gcaagatccc gatcctggct ggcggcgatt gcgctgcggc
     4681 aggtgctctc attggccaag ccattggacg tatcggcgat gtgatcaacg gtgagcacat
     4741 cgccgaatcg agcgacttgg cctgggccgt ggagtacacg cacatcgaca gtcccagttt
     4801 cggattggcg gcgcagcacc cggccgtcgc ctacgagctc cttggtgatc tcctcattct
     4861 tggtgtcgtt ctcgtgctgt ttttccgtgt gcggcgaccc ggatggggct tcttcacctg
     4921 gttcttcggt tacggtttgc tgcgcttcct cgtgagcttc ttgcgtcagg acgaggaggt
     4981 gttgggcgat ctcgtgatgg cgcaggccat tgccatcggg ggcatggcca tcggccttgc
     5041 cggatacgca tggctggcga ttcgtggcga gccagcggga ccgagccgcg cggagcggcg
     5101 ccgcgcccga cgagaggcgg agcagcgcga gcgcgcttcg ccggcgagat agtcgccaga
     5161 gggttggctg tgaggcggag ttcgattgcg cgcctcttga tgggggtgct atacttcgaa
     5221 gcggtggaag caatgaaaag tgggcggaaa ctcccacttt tttgttagcc tttcgcacac
     5281 gcagtaaact ggtggaagaa ccaagcatat gaagaacgac ttcctcgttg ccatctcgca
     5341 gctatcggcg gagaagaatc tgccacgcga ggtcgtgttc gaggccgtgg aaacggcgct
     5401 cgcctctgcc tacaagaagg aggaggtgca cgccaacaac gtcacggcca agatcgatcc
     5461 ggcgaccggc gacgtgcgtg tctgggcggc caaagaggtc gtcgagatcg aagaagatgt
     5521 cgccgatgat cgggaccaga tcccgcttgc cgaggctcgc aagttcgatc cgaacgtcaa
     5581 gatcggggac acactggtct acgaggtgac gcccgctaac gccggccgca tcgccgcaca
     5641 aaccgcgaaa caggtcgtga tgcagcgttt gcgcgaagcc gagcgcgagg tggtattcga
     5701 ggagttcgcg cagcgcgagg gcgatatcgt ctcgggcgtc atcacgaggg tcgagtcgcg
     5761 ccaggtcctc gtcgatctcg gcaagacgga agcggtcatg ccgccaccgg agcaggtgcg
     5821 aagcgagcac tatcgccccg gccagcgcat gaagttctac gttttgcagg tgtttcgagc
     5881 gaacaagggc ccgcaggtca tcgtctcccg aacccacaag gacctcgtaa agcgggtctt
     5941 cgagctcgag gtgccggaga tcttcaaggg tgtcgtcgag atcaaggcca tcgctcgcga
     6001 gcccggctcc cgaacgaagg tagccgtgta ttcgcgtcaa gagggcatcg atgccgtcgg
     6061 agcgtgtgtg gggctgcggg gcgtccgcat tcagaacgtc gtgaacgagc ttcaaggcga
     6121 gcgcatcgat gtcattcagt gggacccgga gccgaggcgc ttcgtcgcca acgcgctgag
     6181 cccggcccag gttctcgcgg tgcgcatcta cgagccgacg aacacggcat acgtggccgt
     6241 acccgatcgg cagctctcgc tcgccatcgg taaggaaggc cagaacgcgc ggcttgcggc
     6301 caagctcacg ggacggcgga tcgatatcaa gagccagacg gcggccgaag aggaatacgg
     6361 cgggttggag ccacccctgc ccgaggagcc ggccgtggcg gttgcgtcca tctccgagcc
     6421 cgctatcgaa gcgggtgtga ccgcagagcc gcacgcggac gtcgaggtgg ccgaggttga
     6481 ggacgaattc gctcttccgc ccgaggaaga ggctgcgctc gcgttcgagg agcctgtgcc
     6541 ggtggaggtg ccaccaaccg aacaagagcc ggttgtcgct gccgagcggg gcaaacctgc
     6601 cctgcgcttc gccgaggaca tcctcggggc gcccgaacgc gccggagacc gtcgtcgtcg
     6661 ccgggagttc gatgagccgg aagtgcccgc gaagcccgtc aagaagaaga aggcagcgaa
     6721 ggcgcgtcgc cttctgcccg acgaggaaga agaagtgctt gatgaactcg acgatgtcga
     6781 ggacatggaa tatccgggcc tgcgccgtta gcaatggggc gaggcaaatt cagtgatggt
     6841 caggagcatg tccgggaagg cgagacgtat tccgcaacgt acctgcgtcg gttgtcgcga
     6901 ggccacggcc aagcggcaac tgaggcgtgt tgttcgtacg ccggaaggtg gggtcgcggt
     6961 cgatccgagt ggcagagcca acggccgtgg agcgtacgtg cacgacagcg ccgaatgctg
     7021 gcgtgacgcg cttcagcggg atgcgcttgg ccgggcgcta cgcaccacga tctcgcccga
     7081 ggatcgtgct tccctcgagg catatgcggg tgcgcttccg agtgaagcgc cgagcccggc
     7141 tctgccatga cgcgtaacga aaactagcat cggcttcgct cacgcgggcg gggaccgatg
     7201 gaaggatgga cgatagattg gcaacaggaa cccgcccaca aacgacaccg gatcccggtg
     7261 atcgcaaggt cgcactcccg cgcgcgctga ccgtagccga gctcgccgac attcttcagg
     7321 tctccgcgat cgatgtgatt aaggagctca tgaagaatgg cgtcatggcg cgcatcaacc
     7381 aggtcatcga cttcgagacc gcggccatcg tcgcctcggg cttcggtttc gagccggagg
     7441 aagagcgtca gcacgtcgag gaggcggcgg aggcggtgga agcccgcacg agccgccgcg
     7501 tcgtcgacga gagtgcgact catcccaggc ctcccgtcat cgcagtcctt ggccacgtcg
     7561 accacggcaa gacgagcctg ctcgatagaa tccgcagtac ggatgtgact tcgggcgagg
     7621 caggcggcat cacccagcac atcggcgcat accaagtcga ggttggcgat cgtgtgctga
     7681 cgttcctcga tacgccgggc cacgaagcgt tcacgcagat gcgggcccgc ggcgcgcagg
     7741 tcacggatgt cgccgtgctc gtcgtggcgg cggacgatgg ggtcatgccg cagaccgtcg
     7801 aagcgatcaa tcacgttcgc gccgctggtg tcccgatggt cgtggcgctc aacaagatcg
     7861 acgtgccgaa cgcgaacccg gaccgggcca agcagcagct catggagcac ggcgtcgtgg
     7921 tcgaagagta cgggggcgat acggtctgcg ttcctgtctc ggcgcggacg ggcgacggta
     7981 tcgatgacct gatcgagagc ctcatcctcg tgagtgacct cttcgagctc aaggcgaacc
     8041 cggaccgccc agcggtcggc gtcgtcatcg aggcaaagct cgatctgcag cgcggaccgg
     8101 tcgcgaccat ccttgttcaa acgggaacgc tgcacgttgg ggattcggtg atcgttggcg
     8161 aaacgtgggg caagctcaag gcgatgttcg acgataaggg ccagcgcgtg aaatccgcgg
     8221 gcccatcgaa gcctgtcgaa atcatggggc tacaggatgt acccatcgcg ggcgaccagc
     8281 tcacggtggt cgacagtgac agggatgcgc gggctcaggt cgagaccatg cgtcgggaga
     8341 aggccgcgga ggccctcaag gagcggc
//
LOCUS       DATMBB010000140         3367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_165151, whole genome shotgun sequence.
ACCESSION   DATMBB010000140 DATMBB010000000
VERSION     DATMBB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165151"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VNL92_02985"
     CDS             <1..374
                     /locus_tag="VNL92_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWO93705.1"
                     /translation="LTEDAWVFDFGELKSIAKELCDELDHKFILQMQSRLLAITEGVH
                     EYEVRFGEGPRYVFPKSDVFPLPKDNSTAERIAEWFVERLAAILADADNVTTIAVGVE
                     EAPGQSGWCRLDVAKGDRRGA"
     gene            complement(403..2322)
                     /locus_tag="VNL92_02990"
     CDS             complement(403..2322)
                     /locus_tag="VNL92_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HWO93706.1"
                     /translation="MTAYLDLRAQAEAAWATWSQPGKPRVLVNDDTSARGQGSRATFA
                     ALEAGIAQRGLDLALQKTGSRGLHYADPVVEVIQPDGASVLYGYVVPERVGELLDALA
                     AGNVATALAIAVVQGPDVPGVPRLEDLPFWKGQVRRLMERCGVIDPDEIDHYLAQGGY
                     AGLARALNMSDEEVIKETLDSGIWGRGGAAFPTGRKWDFLRTARGEPKYLICNADEGD
                     PGAFVNRMLMEGDPHLVVEGMIIGAKATGASYGYIYIRDEYDLAVDRMTRALDQARER
                     GLLGENILGSGLNYDMEVFRGAGAYVCGEETGLISSIDDDRGMPRIRPPFPAQSGVHA
                     KPTNVNNVESYANVPLVFRHNVAWYRQMGTERNAGTKMFSLSGDIERVGVLEVPFGTT
                     LGQVVNDMGGGVPNGVPLKAIQSGGPLGGILPARAVELSLEPEPFRAEGVLLGSGGLI
                     LVSEERCIVDLCIYFLWFAEDESCGRCTTCHGGTQRMVEILERIASGGGRRSDIDNMH
                     LLARTLQWSNCVHGSASPAATETSLTFFRDEYDAHIDEKRCPAHVCAGLIQYEVVQQT
                     ARLPEAADICPTDAFQQQDGAWQINPARCIRCGACRDVAPEAIHVVDRLLPVQGLPGD
                     VVAFAGDHLHAQATP"
     gene            complement(2319..2816)
                     /locus_tag="VNL92_02995"
     CDS             complement(2319..2816)
                     /locus_tag="VNL92_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWO93707.1"
                     /translation="MPQEIPDTREFRQQVQALIQEFKPGGGDLLPALHTIQHEFGYIP
                     RAAAEVIAQHLKLGASDVWGALTFYSELRTVPPADVIVGWCSGPACRLKGGESLRRIF
                     EAELGFPMRTQSIDRKLGLHLAQCNGQCRLAPLVWINGKERGNLTMADGVRLARALKT
                     GELGS"
     gene            complement(2920..>3367)
                     /locus_tag="VNL92_03000"
     CDS             complement(2920..>3367)
                     /locus_tag="VNL92_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HWO93708.1"
                     /translation="LREPLSHVYWMNITDRDLPFIAVVEHTNLIEPERYSGLRIVYLS
                     NYLTPGHEYLTLSPEQLDEIYLPHLSRINPRFSRDWIVDRWVFQAAHAQPVIRCNYRD
                     DLPDHRTPVPGLYLANMQQIYPEDRGQNYSIAMGERVAEMVGQDST"
BASE COUNT          594 a         1106 c         1065 g          602 t
ORIGIN      
        1 ccctgacgga ggacgcgtgg gtgttcgatt tcggcgaact gaagagcatc gcgaaggagc
       61 tctgcgacga gctcgatcac aagttcatcc tgcagatgca gagccgcttg ctcgctatca
      121 ccgagggcgt tcacgaatac gaggtgcggt ttggcgaggg gccccgttat gtcttcccga
      181 agtccgacgt gtttcccctc ccgaaggaca actcgaccgc agagcgaatc gcggagtggt
      241 tcgtcgagcg cctcgctgcc atcctcgcgg atgcggacaa cgtcacgacg atcgcggtgg
      301 gcgtcgaaga ggcgcccggt caatccggct ggtgcaggct cgacgtcgca aaaggtgacc
      361 ggcgcggcgc ttgattctcg gcaaagagca gctgactggt cgctacggcg tcgcctgggc
      421 gtgcaggtga tcgccggcga aagccaccac atccccaggt aggccctgca ccggcaacag
      481 ccgatcgacg acgtgaatgg cttcgggcgc gacgtcccgg caagcgccgc agcgaatgca
      541 acgagccggg ttgatctgcc acgcgccgtc ctgttgctgg aacgcgtccg tcggacagat
      601 gtcagcggcc tcaggcagtc gagccgtctg ctgcacaacc tcgtactgga taagacctgc
      661 acagacatgg gcgggacagc gcttctcgtc gatgtgcgcg tcgtattcgt cccggaagaa
      721 cgtgagcgaa gtctcggtgg cggcaggcga agccgaaccg tgcacgcagt tcgaccattg
      781 cagcgtccgc gcgaggagat gcatgttgtc gatgtcgctg cgacgccccc ctccggacgc
      841 gatccgctcc aagatctcga ccatccgctg cgtgcctcca tggcacgtcg tgcagcgccc
      901 acatgactcg tcctcggcga accagaggaa gtagatgcac aggtcgacga tgcagcgctc
      961 ctcgctgacg agtatgagcc caccgctacc cagaagcacg ccctccgccc ggaacggttc
     1021 tggctccaac gacagctcga cggcacgagc cggaaggatg ccaccaagcg gcccgcccga
     1081 ctgtatcgcc ttcaacggga cgccgttggg tacgcccccg cccatgtcgt tcacgacctg
     1141 tcccagcgtc gtcccgaagg ggacttccag cacgccgaca cgctcgatgt cgccggagag
     1201 gctgaacatc ttcgtaccgg cgttccgctc ggttcccatc tgccgatacc atgcaacgtt
     1261 atgtcgaaac acgaggggaa cgttcgcgta gctttccacg ttgttcacgt tcgtcggctt
     1321 cgcgtgtacc cccgactggg cggggaacgg cggccggatg cgcggcattc ctcgatcgtc
     1381 gtcgatggac gagatcaggc cggtctcctc accgcagacg taggcgcccg caccacggaa
     1441 cacttccatg tcgtagttga ggcccgatcc aaggatgttc tcgccgagga ggccacgctc
     1501 gcgcgcctgg tccagcgccc gcgtcatgcg atcgacggcc aggtcgtatt cgtcgcgaat
     1561 gtagatgtag ccgtacgatg cgcccgtcgc cttcgccccg atgatcatgc cttcgacaac
     1621 gaggtgcggg tcgccttcca tcagcatgcg attcacgaac gcgccggggt cgccttcgtc
     1681 ggcgttgcag atgagatact tgggctcacc gcgggcagtg cgaaggaaat cccacttacg
     1741 ccctgtcggg aacgcggcgc cgcctcggcc ccaaatgccg gagtccaggg tctcctttat
     1801 cacttcctcg tcgctcatgt tgagggcgcg cgcgaggccc gcgtatccgc cctgggccag
     1861 gtagtggtcg atctcgtccg ggtcgatgac accgcagcgc tccatcaggc ggcgcacctg
     1921 ccctttccag aacggaaggt cctccagcct tgggacaccg ggcacgtccg gcccctgaac
     1981 cacggcgatg gccaatgctg tcgcgacgtt acccgcggcc agtgcgtcta gcagctcgcc
     2041 tacgcgctcc ggcacgacgt acccgtacag cacgcttgcg ccgtcgggct gaatcacctc
     2101 cacgacggga tcggcgtagt gaagcccccg gctgcccgtc ttctgcaacg cgaggtcaag
     2161 tccgcgctgg gcgatgcccg cttccagtgc ggcgaaggtg gccctgctgc cctgccctcg
     2221 cgcgcttgtg tcatcgttca cgagaacgcg cggcttcccc ggctgcgacc atgtagccca
     2281 agccgcctcg gcttgagcgc gcaagtccag ataggcggtc acgagcccaa ctccccagtc
     2341 ttgagcgctc gagcgaggcg cacgccgtcc gccatcgtaa gattgccccg ctccttcccg
     2401 ttgatccaca ccaggggtgc cagccgacac tggccgttgc actgcgccag gtgtaagccg
     2461 agcttgcgat cgatcgattg cgtgcgcatg gggaatccga gctcggcctc gaaaatgcgt
     2521 ctcagcgatt cgccgccctt gaggcggcag gccggcccgc tgcaccaacc cacgatgacg
     2581 tccgcgggcg gcaccgtacg aagctcggaa tagaacgtga gggcgcccca cacgtccgat
     2641 gcgccgagct tgagatgctg agcgatcact tcggcggcgg cgcgcgggat atacccgaac
     2701 tcgtgctgga tggtgtggag ggccggcagc aggtccccgc cgcccggctt gaactcctgg
     2761 atcagagctt ggacctgctg acggaactcg cgcgtgtccg gtatctcttg cggcatgcga
     2821 ctcgtctcaa gcagtgggaa cgtccacgcg ggggttgtgc ggaagcgcac taatcatatc
     2881 ccctacccct tcaggcgtac aagcgccttt gtgcgcatat tatgtcgagt cctgcccgac
     2941 catctccgcg acacgttccc ccatcgcgat gctgtagttt tggccgcggt cctccgggta
     3001 aatttgctgc atgttcgcga gatagagacc gggcaccggc gtgcgatggt ccggcaagtc
     3061 gtcccgatag ttgcatcgga tgacgggctg cgcatgcgct gcctggaaca cccaccgatc
     3121 gacgatccag tcgcgcgaga accgcggatt gattcgggag agatggggca ggtagatctc
     3181 atcgagctgc tccggcgaga gggtaaggta ctcgtgtccg ggcgtaaggt agttggaaag
     3241 atagacgatg cgcagtccgc tatagcgctc cggctcgatc agattcgtgt gctcgacgac
     3301 tgcgatgaac ggcaggtccc gatcggtgat gttcatccag tagacgtgcg acaggggctc
     3361 tcggagg
//
LOCUS       DATMBB010000141         3413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_502068, whole genome shotgun sequence.
ACCESSION   DATMBB010000141 DATMBB010000000
VERSION     DATMBB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502068"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VNL92_03005"
     CDS             <1..1756
                     /locus_tag="VNL92_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWO93709.1"
                     /translation="HIEAGRGDQAALIYDSPMTQSVQSFSYSELRDLVARFAGALANL
                     GVTKGDRVLIFMPMVPEALVAMLACARLGAVHSVVFGGFAADELAVRIDDAKPKVIVA
                     ASCGLEPGRVIDYKPLYDGALTQSRHKPDRVVVLQRPQGPVGLEPNRDIDWHEAMRDA
                     SPHPCVSVEATDPLYIVYTSGTTGQPKGVVRDNGGHAVALKWSMKNVYGVDPGDVYWA
                     ASDVGWVVGHSYIVYGPLLHGCTTILYEGKPVGTPDPGAFWRVVAQHGVRALFTAPTA
                     FRAIKREDPQGEHMRRYDLSRFELLFLAGERADPDTVRWAEEQLKVPVLDHWWQTETG
                     WAIVANCAGLGLQPVKHGSPTKAVPGYDVRVLDNEGDEVPRGQIGNLVVKLPLPPGCL
                     PTLWNNDDGFVQSYLDEFPGYYKTADAGFQDEDGYFWIMSRTDDIINVAGHRLSTGGM
                     EEVLASHPAVAECAVVGVADELKGQVPIGLVVLKAGVTLPPEQVCAEVVSLVRQHIGP
                     VASFKTACVVQRLPKTRSGKVLRGTIRRIADRQEYKVPPTIDDPAILDEIEEAMAGIG
                     YGRARTGVVEAAVGMDDE"
     gene            1842..2033
                     /locus_tag="VNL92_03010"
     CDS             1842..2033
                     /locus_tag="VNL92_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HWO93710.1"
                     /translation="MKVRVDHDLCEGNARCMEAAPEVFEVREDEKSYVLIEQPGEELR
                     AKVERAAMLCPKRAVFIDE"
     gene            2134..>3413
                     /locus_tag="VNL92_03015"
     CDS             2134..>3413
                     /locus_tag="VNL92_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWO93711.1"
                     /translation="MTTETRTTSDWQTHYRERLVSGPEAVSHVKSGDKIWISVGQQIH
                     LLLASLVGRADELEGVEIKWLPSSDFGFYTEEMRRHLSINVTYATPFCREAVNNGIAD
                     YTPYPIIGAHKALDEGRPGARPIDVALVCVTPPNKWGYCCFGSNLWDAKTTAQRAQLV
                     IAEVNERLPRTFGDTWIHASEIDWFVENTPPPPEGNWLYPEPDPWDRPIAEYVASLVR
                     DGDTIQIGTGSTTGNIPRLGVLDDKKDLGYFAELTVPGTVDLVRKGVITSSRMTTHPG
                     KFVTTTAGNSPEDLDFIDQNPMFEFYSVEYIHHPGNISKNDNLVAINNALTVDFTGQI
                     AAMSYGTHTYSGTGGHISFAMGAYLSNGGRYICVLPATARGGTVTRIVPQFEPGQVVT
                     VPRDMADYVVTEYGIATLLNKTQRERAQELIAIA"
BASE COUNT          614 a         1091 c         1143 g          565 t
ORIGIN      
        1 gcatatcgag gccggtcgcg gcgaccaggc cgctctcatc tacgacagcc cgatgaccca
       61 gtccgtgcag tcgttcagct acagcgaact gcgcgacctc gtggcgcgct ttgccggggc
      121 gctcgcgaac ctcggcgtga ccaagggcga ccgcgttctc atcttcatgc cgatggtgcc
      181 ggaggcgctt gtcgccatgc tggcttgcgc gcggctgggc gccgtgcact ccgtcgtgtt
      241 cggcgggttc gccgccgacg agctcgccgt gcggatcgac gatgcgaagc ccaaagtgat
      301 cgtcgccgcg tcgtgcggtc tcgagcccgg gcgggtgatc gattacaagc cgctctacga
      361 tggtgcgttg acgcagtccc ggcacaagcc cgaccgtgtc gtcgtcttgc agcgacccca
      421 gggtcctgta ggcctggagc cgaaccggga catcgattgg cacgaggcga tgcgcgatgc
      481 ctcgcctcat ccatgcgtga gcgtggaagc cacggacccg ctctacatcg tgtacacgtc
      541 cgggacaacg ggtcagccga agggcgtcgt gcgcgacaac gggggacacg ccgtcgcgct
      601 caagtggagc atgaagaacg tgtacggagt cgacccgggc gacgtgtact gggcggcctc
      661 cgacgttggt tgggtggttg gccacagcta catcgtgtac ggcccgctgc tccatggctg
      721 cacgacgatc ctgtacgaag gcaagccggt cggcacgccc gaccccggcg ccttctggcg
      781 agtggtcgcg cagcacggcg tgcgggcgtt attcaccgcg cccacggcat ttcgggccat
      841 caagcgcgag gatcctcagg gcgagcacat gaggcggtac gacctttcgc gcttcgagtt
      901 gctgttcctc gcgggggagc gcgcggaccc ggacacggtc cgctgggccg aagagcaact
      961 gaaggtaccg gtgctcgacc attggtggca gacggagacg ggctgggcca tcgtcgcgaa
     1021 ctgcgccggc ctcgggctcc agccggtcaa gcacggctcg ccgacgaaag ccgtgcccgg
     1081 atacgacgtc cgcgtgctcg acaacgaggg cgatgaggtg ccccggggac agatcggcaa
     1141 cctcgtcgtc aaactaccgc tgccgcccgg ctgtcttccc acgctgtgga acaacgatga
     1201 cggctttgtt cagtcgtacc tggacgagtt cccgggctac tacaagacgg cggatgcggg
     1261 attccaggac gaggacggct acttctggat catgagccgg accgacgaca tcatcaacgt
     1321 ggctggccac cgactctcga ccggcggcat ggaggaggtg ctcgcatcgc atccggctgt
     1381 cgcggaatgt gccgtcgtcg gcgtcgccga tgagctcaag ggccaagtac cgatcggtct
     1441 cgttgtgctg aaggccggtg tgacgctccc gccggagcaa gtgtgtgccg aggtcgtttc
     1501 cctcgtacgt cagcacattg gaccggtggc ctcgttcaag accgcgtgcg tggtccagcg
     1561 cttgccgaag acgcgctccg gtaaggtgct gcgcgggacc atccgccgca tagccgaccg
     1621 gcaggagtac aaggttcctc cgaccatcga cgatccggcg atcctggacg agatcgagga
     1681 agccatggcc ggaatcgggt acggccgagc tcgcacgggc gtggtcgagg cggccgtcgg
     1741 catggacgat gaatgagtcc ctggcggacc cggcgagcga ccggtgtcgc gactgagctc
     1801 cggtggaatg accctctgct ggacgtagaa ggaggcacgc cgtgaaggtg cgcgtggacc
     1861 acgacttgtg cgaggggaat gctcgctgca tggaggcagc gccggaggtg ttcgaggtcc
     1921 gcgaagacga gaagtcctac gtccttatcg agcaacccgg cgaggagctg cgcgccaagg
     1981 tggagcgggc tgccatgctc tgccccaagc gtgccgtctt tatcgacgag tgaggtagcc
     2041 ggccacagca ttcggggctt ggcatgcccg ctatactgcg cgcaataggc tggccagcat
     2101 gatgccgcga aggtgcgcgg cggaggggga gcgatgacga ccgaaacgcg gacgaccagc
     2161 gattggcaga cgcactatcg agagcggctg gtgagtgggc cggaagctgt ctcgcacgtg
     2221 aaatccggcg acaagatctg gatatccgtc gggcagcaga tccacctcct gctggcctcc
     2281 cttgtcggcc gtgcggacga gctggagggc gtcgagatca agtggctccc ctcctccgac
     2341 ttcgggttct acaccgagga gatgcggcgg cacctgagca tcaacgtcac gtacgccacg
     2401 ccattctgcc gcgaggctgt gaacaacggc atcgccgatt acacgccgta tcccatcatc
     2461 ggcgcgcaca aagccctcga cgaaggccgc cctggggcgc ggccaatcga cgtcgcgctc
     2521 gtctgcgtca cgccgcccaa caagtggggg tactgttgct tcgggtccaa cctctgggat
     2581 gcgaagacga ccgcccagcg ggcccagctc gtcatcgccg aggtgaacga gcgcttgccg
     2641 cggacgttcg gcgatacgtg gatccatgcg agcgagatcg actggttcgt cgagaacacg
     2701 cctccaccgc cggagggaaa ctggttgtac ccggagcccg atccctggga tcgccccatc
     2761 gccgagtacg tcgcttcgct cgtccgtgat ggcgacacga ttcagattgg caccggctcc
     2821 acgacgggga acattccccg actcggcgtc ctcgacgaca agaaggacct gggctacttc
     2881 gcggagctca cggtccccgg caccgtggac ctcgtgcgca agggagtcat cacctcgagt
     2941 cgcatgacga cccaccccgg caagttcgtc acgacgacag caggcaattc tccggaggac
     3001 ctcgacttca tcgaccagaa cccgatgttc gagttctatt cggtcgagta cattcaccac
     3061 cctgggaaca tctcgaagaa cgacaatctg gtggcgatca acaacgcgct cacggtcgat
     3121 ttcacggggc agatcgctgc gatgtcctac ggcacacaca cctacagcgg taccggcggg
     3181 cacatctcgt tcgccatggg tgcgtatctt tccaatggcg gccgatacat ctgcgtcctg
     3241 ccggcgacgg cgcgcggggg caccgtgacg cgcatcgtgc ctcagttcga gccgggccag
     3301 gtggtcacgg tgccgcgcga catggcggac tacgtcgtga cggaatacgg aatcgcgact
     3361 ctgctcaaca agacgcagcg cgagcgggcg caagagctca tcgccatcgc cca
//
LOCUS       DATMBB010000142         2585 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_408880, whole genome shotgun sequence.
ACCESSION   DATMBB010000142 DATMBB010000000
VERSION     DATMBB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408880"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VNL92_03020"
     CDS             complement(<1..202)
                     /locus_tag="VNL92_03020"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246473.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWO93712.1"
                     /translation="MSILVGKETRLLVQGITGKEGSFQTERCIQYGTTVIGGVTPGRG
                     GETVLDRPVFDTVGDCVRQTGAN"
     gene            complement(218..985)
                     /locus_tag="VNL92_03025"
     CDS             complement(218..985)
                     /locus_tag="VNL92_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93713.1"
                     /translation="MTTDPRLRFAPTAVNYVRSAIHAQGDDLPKLAALAERLQPALVL
                     DVATGGGHTALAVAPHARHVIAYDLTREMLQAAREYIAARGAPNVVLVEGDAAHLPFA
                     SGAFDLVTCRVASHHFADVPAFCREAARVLRRSGSLAIVDILGHEDATFDEFLHDIEV
                     RRDPSHVRNLPPSAWRRILREAGFSVSTIETFRRLHDYEDWMERQHVPPNTRVEIARD
                     MLAAPPSVREHYRIEPGGPSGVESFETDLGLVLAVRN"
     gene            complement(982..2145)
                     /gene="sucC"
                     /locus_tag="VNL92_03030"
     CDS             complement(982..2145)
                     /gene="sucC"
                     /locus_tag="VNL92_03030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWO93714.1"
                     /translation="MKIHEYQAKQLFHEFGIPVPRGHVASTPQEAKLIAQDLGGHVVV
                     KAQIHAGGRGKGGGVKLANSIDEAEQAASAILGMQLKTHQTGPEGQRVKSVLIEEQMQ
                     PDRELYVAVLLDNAIGWPILMGSAAGGMDIEEVAATTPEKIVRIPIDPATGFQPFHGR
                     RLAFGMDLRGDLLRPAVQLFGGLYNLYLARDCSLVEVNPLVVTSDGRLLALDAKVNFD
                     DNALFRHKDIEELRDKDEEDPLEVRAQELGINNYVKLDGNIGCVVNGAGLAMATMDSI
                     KLAGGEPANFLDIGTVNDVGRVVNAFRILTSDPAVRAVLINIFGGMARVDIIAQGIIE
                     AHQQMHVEVPVVVRLAGTNVEEGERILAEAGDVRVQRAKDLGEAAKLAVAAAR"
     gene            complement(2254..>2585)
                     /locus_tag="VNL92_03035"
     CDS             complement(2254..>2585)
                     /locus_tag="VNL92_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93715.1"
                     /translation="ASLVGDASAEDTRAELSAHIDELAREAMLTGADPSTAYADALRA
                     MGEPRLLARALREARELPPLRPAPAGYTQARLRTREAWQRRAMVWLVLGALMLQLNVL
                     AFLYLYP"
BASE COUNT          400 a          846 c          875 g          464 t
ORIGIN      
        1 agttggcccc cgtttgccgg acgcaatccc ccaccgtatc gaagaccggc ctatcgagca
       61 ccgtctcgcc gccgcgcccc ggcgtcaccc cgccgatgac ggtggttcca tactggatgc
      121 agcgttccgt ttgaaagcta ccttccttcc cggtgatacc ctgcaccagc aaccgtgttt
      181 ccttgccgac gaggatgctc actatcgtcc tcctgcttca atttcgtacc gctaagacga
      241 gaccgaggtc cgtctcgaaa ctctcgactc ccgatggtcc gcccggctcg atgcgatagt
      301 gttcccggac cgatggtggc gccgcgagca tatcccgggc tatctccaca cgcgtgtttg
      361 gcggcacgtg ctgccgctcc atccagtcct cgtagtcgtg caggcgacgg aaggtttcga
      421 tggtgctcac gctgaagcct gcttctcgca ggatgcggcg ccatgccgat ggtgggaggt
      481 tccggacgtg ggatggatct cgccggacct cgatgtcgtg caggaactcg tcgaacgtgg
      541 cgtcctcatg cccgaggata tccacgatgg ccagggagcc ggagcgccgg agcacgcggg
      601 cggcctcgcg acagaatgcg gggacatcgg cgaagtggtg cgaggcgacg cgacaggtga
      661 ccagatcgaa cgcgccgctt gcgaagggga gatgcgccgc gtcgccctcg accagaacga
      721 cgttcggggc gccgcgagcg gcgatgtact cccgcgcagc ctgcagcatc tcccgcgtga
      781 ggtcgtacgc gatgacgtgc cgtgcgtgcg gcgctacggc gagcgccgtg tgcccaccgc
      841 cggttgcgac atcgagcacc agcgccggtt ggagacgctc cgcgagggcg gcgagcttcg
      901 gtaggtcatc gccctgggcg tggatggcgc tgcggacgta gttgaccgct gtcggtgcga
      961 atcgtaggcg cgggtccgtg gtcaccgcgc ggctgccaca gcgagcttcg cggcctcgcc
     1021 gaggtccttt gctcgctgca cccgcacgtc gcccgcctcg gccaggatgc gctccccctc
     1081 ctcgacgttc gtgcctgcca aacgtacgac gactggaact tcgacgtgca tctgctgatg
     1141 cgcctcgatg atgccctggg cgatgatatc tacccgcgcc atgccaccga agatgttgat
     1201 gaggacggct cgaactgccg ggtcggacgt gaggatgcgg aaggcattca ccacgcgtcc
     1261 gacgtcgttc acggtgccga tgtcgaggaa gttggcaggc tcgccgcctg caagcttgat
     1321 gctatccatg gtcgccatgg ccagccccgc gccgttcacc acgcagccga tgttcccatc
     1381 gagcttcacg taattgttga tgccgagctc ctgggcgcgg acctcgagcg ggtcctcctc
     1441 gtccttgtcg cggagctcct cgatgtcctt gtgccggaaa agcgcgttgt cgtcgaagtt
     1501 gaccttcgcg tcgagtgcca gcagtctgcc gtcggacgtg acgacgagcg ggttcacttc
     1561 gacgagcgag cagtcgcgag ccaaatacag gttgtagagg ccgccaaaca gctgcaccgc
     1621 gggccgcagg aggtctccgc ggaggtccat gccgaaggcg agcctgcggc catggaacgg
     1681 ctggaacccc gttgccggat cgatcgggat gcgtacgatc ttctcgggtg tggtagccgc
     1741 cacttcctcg atgtccatgc ccccggcggc ggaccccatg aggatgggcc agccgatggc
     1801 gttgtccagg aggaccgcaa catagagctc gcggtcaggc tgcatctgct cctcgatgag
     1861 gacgctcttg acgcgttgcc cttccggtcc cgtctgatgg gtcttgagct gcatgccgag
     1921 aatggcggac gcagcctgtt cggcctcgtc gatcgagttc gcgagcttga cgcccccgcc
     1981 ctttccgcgt ccgcccgcgt gaatctgcgc tttgaccacg acatgcccgc cgaggtcctg
     2041 cgcgatgagc ttggcctcct gcggtgtgga ggcgacatgg ccccggggca ccgggatgcc
     2101 gaactcgtgg aacagttgct tcgcctggta ctcgtggatc ttcacgtgcg cctccgcagg
     2161 gagatgggga acccttcggg ttatagcgca ggcgacgagt gcggggcaag ctcgaccgct
     2221 gcgctatggt tgcggacggg cgagcgctgg cgtctacgga tacagataga ggaaggcgag
     2281 cacgttcagc tgcagcatca gggcgcccag gaccagccag accatggccc ggcgctgcca
     2341 agcctcgcgg gttcgcaagc gagcttgggt gtagccggcc ggcgccggac gtagcggcgg
     2401 gagctcgcgg gcctcgcgga gcgccctcgc gagcaggcgg ggctctccca tggcgcggag
     2461 agcgtcggcg taggccgtgc tcgggtcggc gcctgtgagc atcgcctccc tcgccagttc
     2521 atcgatatgg gcggatagct cggcgcgcgt gtcctccgcc gacgcgtcgc ccacgaggga
     2581 ggcgc
//
LOCUS       DATMBB010000143         5054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_326204, whole genome shotgun sequence.
ACCESSION   DATMBB010000143 DATMBB010000000
VERSION     DATMBB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326204"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VNL92_03040"
     CDS             complement(<1..387)
                     /locus_tag="VNL92_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO93716.1"
                     /translation="MTTKSNRRVPIEPGYFTVPENPNEAPRLLGSRCRACGEHFFPRR
                     VVCAKCLARDIEDVELSPRGKLYTWTHVFVPMFGAQRTDLNEYDVGQVDLPEGVRVQT
                     TLTGDTSKLRIGGEMEICLETLRQDDE"
     gene            complement(483..1394)
                     /locus_tag="VNL92_03045"
     CDS             complement(483..1394)
                     /locus_tag="VNL92_03045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO93717.1"
                     /translation="MPQLKFGAMAGGEAARQARHLEDLGYESLWVGGHIVFYGPMPEQ
                     LTQLAALAMTSERATIGTNVLVLPLYHPTIVAKQIATIDHLSHGRVILGVGVGGEYAK
                     EYEAVEVPREQRGSRANEGIELIRKLWTGEPVSHHGKHYNLEGIRMQPPPVQQPGPPI
                     WVGGRSEAAARRCGRYGDGYIPYMVSIDHYPQRLATIRETAEANGRDAGAIERAIFIF
                     GCLSDDPVQAADIVKTRLGLMYNQSFEGIIEKYCIYGTAEQCVAGVERFAEAGVQHFV
                     IAPMPAGEADTSPLDRWMTDVFARFRS"
     gene            complement(1398..2480)
                     /locus_tag="VNL92_03050"
     CDS             complement(1398..2480)
                     /locus_tag="VNL92_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93718.1"
                     /translation="MEGGLHGWRRAVHLAGAVLLLVAVNITVGVPWQSLGPHNEGMEL
                     GLNFSCRRARHLMSDIDCLSLFETMLTELQPGHVRLALYWDELEPERGVFDYSSVDPY
                     LRLAEQHDVGVVPIIGVKSTYRPEFYIPEWVASTILWGAPPSDDDRIAEASLAMLAQV
                     VGHLQAAPAIEAWQVENEPDVHPDGDARGWTVPNSFLAREVAVVRDADPAGRPVILTD
                     DTSIFYQREWIDLLALGDGLGLGFYPRRPGGVFPWQDELRRFDMGALGSDHWNVMTRA
                     RLEGKQAWIVEVQAEPWQTRPASELRPDEITSVSPEIMRENVDLASRTGAERAYLWGV
                     EWWWFQRVRWQDDSYWDTARDLLRGN"
     gene            complement(2502..3761)
                     /gene="ccrA"
                     /locus_tag="VNL92_03055"
     CDS             complement(2502..3761)
                     /gene="ccrA"
                     /locus_tag="VNL92_03055"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HWO93719.1"
                     /translation="MSTAVKDLYEIGEMPPLGHVPARMYANLIPENARAQPKDGFKVR
                     EVPVPSDLKPDEVLVYVMAAGVNYNNVWASLGVPLDMAAIHRKEGDDSGFHIGGSDAS
                     GIVWATGSDVTNVKVGDAVVIHCGQWDRDDPWVKAGGDPILAPSTKIWGYESNWGSFA
                     QFTKVQGHQCLPKPAHLTWEESAAYMLVGATAYRMLAAWPPHTVQPGDVVLVWGGAGG
                     LGVMAIQLARLMGGTPVAVVSSESKFEYCMKLGAKGCINRKNFDHWGLPPHWDDEAAY
                     GAWLQGARAFGKAIWDVVGERKNPRIVFEHPGEDTVPTSIFVCDNGGMVVICAGTTGY
                     SAMVDLRYLWMRQKRFQGSHFANDEQCAAFNQLVIEGKVDPCVSRVFPFEEIGLAHQL
                     MYENKHPEGNMVCLVGAPATGLKDIPS"
     gene            3881..4447
                     /locus_tag="VNL92_03060"
     CDS             3881..4447
                     /locus_tag="VNL92_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93720.1"
                     /translation="MRTVSSAAALMGGALGLLAGAIVGAIIVIAGDDEPADGWRSIIA
                     LVAAAPHALAIAAAYASRPAARMVLLLVASTAGLLLSFALYTVGPLFWPAVVLELIAT
                     VRAFRKSGSSGLGDLMRPLSSLGAAFLFVIAFFALFLHDDPRCARSSCTSDVVTASEA
                     MLALVLTGAGLALAVWIARDESRRVALR"
     gene            complement(4651..>5054)
                     /locus_tag="VNL92_03065"
     CDS             complement(4651..>5054)
                     /locus_tag="VNL92_03065"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HWO93721.1"
                     /translation="VALIFFFVALQLLSGADYLDVFETSHLDAQALVALDAFNYTWLI
                     GLAAFGVHLGVMGYLILQSGEVTQALGYLLIIAGAAYITDTIANALLSNYDDFEALFL
                     TIVVIPSVIAELWLTIWLLTRAGREPTLHLS"
BASE COUNT          857 a         1695 c         1608 g          894 t
ORIGIN      
        1 ttcatcgtcc tgacggagcg tctcgaggca gatctccatc tccccgccga tccgcagctt
       61 cgacgtatcg cccgtcagtg tcgtctggac tcgtaccccc tccggcaggt caacctgtcc
      121 cacgtcgtac tcgttgagat cggtgcgctg tgccccgaac atcggcacga aaacgtgcgt
      181 ccaggtgtag agcttcccgc gcgggctgag ctccacatcc tcgatgtcgc gcgccaggca
      241 cttagcgcag acaacacgac gcggaaagaa gtgctccccg caggcgcggc agcggcttcc
      301 aagcaggcga ggcgcttcgt tcggattctc gggaacggtg aagtagccag gctcgatggg
      361 cactcgcctg ttgctcttcg tggtcattga tccctcccag cgagagttcg cgcggacatc
      421 gcgcgtgcgg gcggcgcaag aatacccgca acgccgccgg agcgtccaga cttgggggaa
      481 gcctaggatc ggaatcgagc gaagacgtcg gtcatccagc ggtccaacgg gctggtatct
      541 gcttcgccgg cgggcatcgg cgctatgacg aagtgctgta cgccggcctc ggcgaaccgc
      601 tcgaccccag ccacgcactg ctccgccgtg ccgtagatgc agtacttctc gatgatgccc
      661 tcgaacgact ggttgtacat gaggccgaga cgagttttca cgatgtccgc cgcctggacc
      721 ggatcatcgg agaggcagcc aaagatgaag atcgctcgtt cgatggctcc ggcatcccgc
      781 ccgttcgcct cggccgtctc tcggatggtg gcgagccgct gcggatagtg gtcgatgctc
      841 accatgtacg gaatgtaacc atcgccgtag cgtccgcagc gccgcgctgc tgcctctgac
      901 ctgccgccga cccaaatcgg cgggcctggc tgctgcacgg gcggaggttg catgcgaatg
      961 ccttcgaggt tgtagtgctt gccgtgatgg ctcaccggtt cgccggtcca cagcttgcgt
     1021 atgagctcga tgccttcgtt cgcgcgcgag ccgcgttgct cccgcggcac ctccaccgcc
     1081 tcgtactcct tcgcgtactc gccgccgacg ccgacaccga ggatgacgcg gccgtgcgaa
     1141 agatgatcga tcgtcgcgat ttgctttgcg acgatcgtcg ggtgatacag cggcagaacc
     1201 aggacgttgg tcccgatcgt cgcccgctcg ctcgtcatcg cgagggccgc gagctgcgtg
     1261 agctgctccg gcatcggacc gtagaacacg atgtgcccgc cgacccacag cgactcgtaa
     1321 ccaagatcct ccaggtgccg cgcctgacgc gccgcctcgc cgcccgccat cgcgccgaac
     1381 ttcagctgtg gcatcgctca gttccccctc aacagatctc gcgccgtgtc ccaatagcta
     1441 tcgtcctgcc agcgtacgcg ctggaaccac caccactcta cgccccagag atatgcccgc
     1501 tccgccccag ttcggctggc gagatcgacg ttctcccgca tgatctccgg agagacggac
     1561 gtgatctcat cggggcgaag ctccgaggcg ggccgcgttt gccacggctc cgcctgaacc
     1621 tcgacgatcc acgcctgctt cccctccaac cgtgcgcgcg tcatcacgtt ccagtgatcg
     1681 gagccgagag cccccatatc gaagcgacgg agctcgtcct gccaagggaa cacgccgccg
     1741 ggtcgtcgcg gatagaagcc gagaccaagg ccgtcgccaa gggcgagcaa gtcgatccac
     1801 tcccgttggt agaagatgga cgtgtcgtcg gtaaggatga cggggcgacc agccgggtcc
     1861 gcgtcgcgga ccacagcgac ctcgcgggca aggaacgagt tcggcaccgt ccagcctcgt
     1921 gcgtcgccat cagggtgaac gtcgggctcg ttctccacct gccacgcctc gatcgccggg
     1981 gccgcctgca agtgtccgac cacctgcgcg agcatcgcga gcgaggcctc tgcgatgcga
     2041 tcgtcgtcgg aaggcggcgc accccacaga atcgtgctcg ccacccactc cggaatgtag
     2101 aactcggggc ggtaggtgct cttcaccccg atgatgggca cgacccccac gtcatgctgc
     2161 tctgcgaggc gcaggtacgg gtcgaccgac gagtaatcga agacacctcg ctcgggctcg
     2221 agctcatccc aatacagcgc cagtcgcaca tggccgggct gcagctccgt gagcatggtc
     2281 tcgaacaagg acagacagtc gatgtcagac atgagatgtc gggctcgccg gcaactgaag
     2341 tttaggccca actccatccc ctcgttgtgc ggaccgaggc tctgccaggg cactccgacc
     2401 gtgatgttga ccgccacgag caagagtacg gcgcccgcca ggtgcactgc gcgccgccat
     2461 ccgtgcaggc ctccctccat cgtcccgact gggcggcacg ctcaggaggg gatgtccttc
     2521 agccccgtgg ccggcgcccc gacgagacag accatgttgc cttccggatg cttgttctcg
     2581 tacatcagct ggtgcgcgag gccgatctct tcgaacggga acacccgcga gacgcaaggg
     2641 tcgaccttgc cctctatgac gagctggttg aacgccgcgc actgctcgtc gttcgcgaag
     2701 tgcgagccct ggaatcgctt ctgccgcatc cagaggtacc gcaggtcgac catggcgctg
     2761 tagccggtcg tcccggcgca gatgacgacc atgccgccgt tatcgcacac gaagatcgag
     2821 gtcgggaccg tgtcctcgcc cggatgctcg aagacgatgc gcgggttctt gcgctcgccc
     2881 accacgtccc aaatcgcttt gccaaaggcg cgggcaccct ggagccaggc tccgtacgcg
     2941 gcctcgtcgt cccagtgggg gggcaagccc cagtgatcga agttcttcct gttgatgcac
     3001 cccttggcgc ccagcttcat gcagtactcg aacttcgact cgctcgagac tacggcgacc
     3061 ggtgtaccac ccatgaggcg cgccagctgg atggccatga cgccgagtcc acccgcccct
     3121 ccccacacga gaacgacgtc ccccggctgc accgtgtggg ggggccaggc ggcgagcatt
     3181 cgatacgcgg tggcgccgac cagcatatag gccgccgact cctcccacgt gaggtgcgcg
     3241 ggcttcggca gacactggtg accttgcacc ttcgtgaatt gggcaaagga accccagttc
     3301 gactcgtacc cccagatctt ggtcgaaggc gcgaggatcg ggtccccgcc ggccttcacc
     3361 catgggtcgt cgcggtccca ctggccacag tggatgacca cggcgtcgcc gaccttcacg
     3421 ttggtgacat cgctgcccgt agcccacacg atgccggagg cgtcgctgcc gccgatgtgg
     3481 aaacccgagt cgtcgccctc cttacggtgg atggccgcca tgtcgagggg caccccgagg
     3541 ctggcccaca cattgttgta gttgacgccg gcagccatca cgtacacgag cacctcgtcg
     3601 ggcttgaggt cgctgggaac cggcacctcg cgcaccttga agccgtcctt cggctgcgcc
     3661 cgggcgttct cgggaatgag gttggcgtac atgcgagccg gaacgtgccc gagtgggggc
     3721 atttcgccga tttcgtagag gtccttgacg gccgtggaca tagcgcgcct ccatcgatgg
     3781 tgtcagaggc tgactgtaca ggattggggg gcgccccaca accgagggcg tggcgccggc
     3841 gggctcgcac ctggccaatc cactgcgtac cataagggcg atgcgtacgg tcagctcggc
     3901 tgcggcgctg atgggcggag cactcggcct cctcgcgggc gcaatcgtcg gcgccatcat
     3961 cgtgatcgcc ggcgacgatg agccggcgga tggctggcga agcatcatcg ctctcgtggc
     4021 ggccgcgcct catgcgctcg caatcgccgc cgcctacgcg tctcgacccg cagcgaggat
     4081 ggtgctgttg ctggtcgcgt caaccgccgg gctgctactt tcgttcgcgc tctacaccgt
     4141 ggggccgctc ttctggcccg cggtcgtact cgaactcatc gcgaccgtgc gcgcattccg
     4201 gaaatcgggc tcctccggac tcggcgacct catgcgcccg ctcagctcgt taggcgcggc
     4261 cttcctgttc gttatcgcgt tcttcgcgct gttcctgcac gatgatccgc gctgcgcgcg
     4321 gagttcgtgc acaagcgatg tcgtgacggc gagcgaggcc atgctcgcgc tcgtccttac
     4381 gggagcgggc ctggcgcttg cagtctggat cgctcgcgac gaatcgcggc gcgtggcctt
     4441 gcgatagcca cagcgtcgcc gtgccggccc gcgcgcccgt gtatcaatat cctgtgttcg
     4501 gaggaacgcc acatcgagcg cctgcgcgct ccacatcttg cgcgcgagcc gaaccgcgtg
     4561 agcgcgctcg tttacggcgg ctcgggccgc cgtggtacgg tgattgtcgg gtgagactcg
     4621 atgcgctgcg agagagtcct cgcgcaccta tcagctcaga tgcagcgttg gttcgcgccc
     4681 ggcgcgtgtg aggagccaaa tcgtcagcca gagttcagcg atgacagacg ggatgacgac
     4741 gatggtcaga aacagggctt cgaagtcgtc gtagttcgat agtagcgcgt tggcgatcgt
     4801 gtcggtgatg tacgcggcgc cggcgatgat gagcaggtat ccgagcgctt gggtgacctc
     4861 acccgactgc agtatcaggt accccatcac gccaagatgc actccaaagg cagcaaggcc
     4921 gatgagccaa gtgtagttga acgcatcaag ggcgacgagc gcctgtgcgt cgagatggga
     4981 ggtctcgaat acgtcgaggt agtcggcgcc gctgagtagt tggagagcaa cgaagaagaa
     5041 gatgagcgcg acgc
//
LOCUS       DATMBB010000144         3825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_398829, whole genome shotgun sequence.
ACCESSION   DATMBB010000144 DATMBB010000000
VERSION     DATMBB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398829"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VNL92_03070"
     CDS             complement(<1..1222)
                     /locus_tag="VNL92_03070"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HWO93722.1"
                     /translation="MSILFITTAETEVLALDMALRGMSAEGVPPVRAVAARQLTDAAT
                     AQNLLTSRPQTRLVLARLLGGKAGLGEEPFHALAGACRDRGIPFLALPGDSQPDAGLA
                     AASTVPSSIASVAFEYLLHGGTANMEHLVRFLADEMLGTSLGYDPPAPLPWDGLYHPD
                     LPSGALLDQHLVRLKPNAPTIGVLFYRAAWMSGDTAAIDAIIRALEARGANVLCAFCY
                     SLKDEDAASTGAPAVVRRYLLDDAGRPRIDALISTLSFSIGNLNVEGATVASGWSVDY
                     LSKLDVPIIQGVLVSSPREQWLASAAGLSPIDAAMKVAMPEFDGHIIGPPVAFREAAE
                     ASALGTNVLRLVPDEEGVSVIAEQALAWAKLRQTPNARKRVAIVLANYPTKNARIGNA
                     VGLDSTESVVRLLHA"
     regulatory      complement(1309..1508)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1706..2533
                     /locus_tag="VNL92_03075"
     CDS             1706..2533
                     /locus_tag="VNL92_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWO93723.1"
                     /translation="MLAIRPPDLPPPSENDVLYEKLDGFARITLNRPLVLNAIDWSVS
                     RWLYRSLLRADEDNDVRAIILTGAGRAFSSGGDMAADPQPDDGVLTPNIVDIALKIWG
                     MQAPVIAAVAGHAVGQGIELAGICDMTIAADDAKFGEIQIRHGFGPPLLITPFLVGLK
                     QAKEMLLLGDILTAEDALRIGLINRVVPRERLQDEAEGMARKIAALNVDTVRLNKRLV
                     NRAYELAGFLGALDYRSYPELAELFTGSASDSAHLNVLREQGWEAFIRERDKLYRQA"
     gene            2535..3335
                     /locus_tag="VNL92_03080"
     CDS             2535..3335
                     /locus_tag="VNL92_03080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWO93724.1"
                     /translation="MFDDRSAIRNVSSEGVLVLGGGRALLMQVAHPSIAAGVADHSNF
                     RGDRIGRLLRTLEATYALVFGTPEQAEAAARRVNAMHERVVGPGYRALDPDLLLWVHS
                     TLIDTSLLMYSLFVRRLSDREREAYYRDARRMGHAYGLDDDALPRTYRDLDAYVREMV
                     ATLVVNDTARRIAAAIFAPEPPIVAPAMILARELTAELLPPSLRAQYGLHTQPMRTFA
                     LQSAAALSRELLPYVPRPLRAPPPFVMPKGGWSRAAFRRPIAVEASVP"
     gene            complement(3322..3741)
                     /locus_tag="VNL92_03085"
     CDS             complement(3322..3741)
                     /locus_tag="VNL92_03085"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWO93725.1"
                     /translation="MVIKLTEEMREHINNALMNRAPLIIASVDEEGQPHLTFRGSTHV
                     HGDDALAIWARNPDGAFLRHLASNPRVALMYQNFEARVGWQFQGRAHVATDPVEAKRV
                     YDESPEPERSLDPERKGCAVVIVLDRVIQRGTVIMER"
BASE COUNT          639 a         1291 c         1249 g          646 t
ORIGIN      
        1 tggcatggag gagccgcacc acggattcgg tcgagtcgag cccgacggcg ttcccaatgc
       61 gggcgttctt cgtcggatag ttggccagga cgatggcgac tcgcttccgg gcgttggggg
      121 tttgccgaag cttggcccat gccaacgctt gctcagcgat gacgctcacg ccctcctcgt
      181 ccggaacgag gcgcaagaca ttcgtaccca gcgccgacgc ttcggcggct tcccggaagg
      241 ccacgggcgg cccgatgatg tgcccatcga actccggcat cgccaccttc atcgccgcgt
      301 cgatgggact caacccggca gcgctggcga gccactgctc gcgcggcgag gacaccagga
      361 cgccctggat gatcggcacg tcgagcttgc tgaggtaatc gaccgaccat ccgctcgcga
      421 ccgtggcacc ctcgacgttg agattgccga tggagaagct gagggtgctg atgagcgcat
      481 cgatacgtgg ccggccggcg tcatccagaa gataccggcg aacgactgcg ggcgcgccgg
      541 tggaggccgc atcctcgtct ttgagggaat agcagaacgc gcagaggacg ttggctccgc
      601 gcgcctccag cgcgcggatg atcgcatcga tggccgcggt gtcgccgctc atccacgccg
      661 cgcgatagaa gaggacgccg atcgtgggag cgtttggctt gaggcggacc agatgctggt
      721 ccaggagcgc gccactcggg agatcgggat gatacaggcc atcccacgga agaggcgcag
      781 gcgggtcgta gccgagcgaa gtgccgagca tctcgtcggc gagaaagcga acgagatgct
      841 ccatgttagc cgttccgccg tgcagcagat actcgaacgc gacggacgcg atggaggatg
      901 gcacggtcga agcggcagcg aggccggcat cgggttggga atccccgggg agcgcgagga
      961 acggaatgcc gcgatcgcga caagccccgg ccagcgcgtg gaagggctcc tcgcccagtc
     1021 cggctttgcc accgagtagg cgcgccaaca ccaatcgcgt ttgtggccgc gaagtcagga
     1081 gattctgagc cgttgccgcg tcggtcaatt gacgcgcagc tacggcgcgg acggggggga
     1141 cgccctcagc ggacattccg cgcaacgcca tatcgagggc gagcacctca gtctcagccg
     1201 tggtgatgaa caggatcgac accgaaaagc ccgtccttcc ttgggaagca cgggcgtcgc
     1261 gtccttccgc gggacggaag gagtcgccga cgcccactcc tcgaggagcg tatggcgaag
     1321 gctcgtggca ggtctcctgg cttccggctc aggcgacgcc gagcgcgcct tcccggccgt
     1381 ctggcggcca gtggctcccc cgaggggaag gcgtcggcgt ctcgccggtc acagtggcgg
     1441 gaccgcgccg gtctctcacc ggcttcccta ttctgcccat acgggccacc gcgagcgctt
     1501 ccgtatttgg ttgtggcctc gcgcgaggcg gaacgtgggg acgatagcac ggagccgcga
     1561 gcgggtcaac ggcggaaccc gggccctgac gttgcgccgc ccacccgagg attcctcaga
     1621 ggtgttgacg ccatcgcgct gctcgacgca tgcttcgcat gcgatgtccc cgaccgaggg
     1681 gcgtcgacat ttgcaccgag gttcgatgtt ggctatccgg ccgcccgact tgccaccgcc
     1741 ttccgagaac gacgtcctgt acgagaagct cgatgggttc gcgcgcatca cactcaaccg
     1801 gcccctcgtg ctcaacgcga tcgactggtc ggtgtctcgc tggctctacc gatctctcct
     1861 gcgcgccgac gaggacaatg acgtccgcgc catcattctc acaggcgccg gccgggcctt
     1921 ctcctccggg ggagacatgg ccgccgaccc gcagccggac gacggcgtgc tcacaccgaa
     1981 catcgtggac atcgccctca agatttgggg catgcaggcg ccggtcatcg ccgcggtggc
     2041 cggccatgcc gtgggccagg gcatcgagct ggcgggcatc tgcgacatga ccatcgcggc
     2101 cgatgacgcc aagttcggcg agatccagat tcggcacggg ttcggcccgc cgctcctcat
     2161 tacgcccttc ctcgtcggat tgaagcaggc gaaggaaatg ctgctcctcg gcgacatctt
     2221 gacggcggaa gacgccctgc ggatcgggct catcaaccgt gtcgtgcccc gcgagcgcct
     2281 ccaggacgag gccgagggca tggcccggaa gatcgctgcc ctgaacgtgg acacggtgcg
     2341 actcaacaag cgtctcgtca atcgagccta cgagttggct ggcttcctag gagcgctcga
     2401 ctatcggtcc tatcccgagc tcgcggagct cttcacgggc agcgcttccg actccgccca
     2461 tctcaacgtc ctccgtgagc agggctggga ggcgttcatt cgggagcgcg acaagcttta
     2521 tcgtcaggcc taacatgttc gacgatcgat cggcgattcg gaacgtatcc agcgagggcg
     2581 tcttggtcct cggcggtggg cgcgccctgc tcatgcaggt ggcgcatccc tccatcgccg
     2641 cgggcgtggc cgatcacagc aacttccgcg gcgaccgcat cggacgcttg ctgaggacgc
     2701 tcgaagcgac ctacgccctc gtcttcggca cacccgagca ggccgaggca gcggcacggc
     2761 gcgtgaacgc catgcacgag cgagtcgtcg gacccggcta ccgggccctc gaccccgacc
     2821 tgctcctctg ggtgcactcg accctcatcg acacgtcgct cctgatgtac tcgctcttcg
     2881 tccggcgcct cagcgaccgc gagcgcgaag cgtactatcg cgatgcccgc cggatgggac
     2941 acgcctacgg cctcgacgac gacgccctgc cgcgaacgta ccgcgacctc gatgcctatg
     3001 tgcgcgagat ggtcgcgacg ctcgtcgtga acgacaccgc acgtcgcatc gcggctgcca
     3061 tcttcgcgcc cgagcctccc atcgtggcgc cggcgatgat tcttgctcgc gagctcaccg
     3121 cggagctgct cccgccgagc cttcgcgcgc agtacggtct ccatacgcaa ccgatgcgca
     3181 cgttcgcgct gcagagcgct gcggcgctca gtcgcgagct cctcccgtac gttccccggc
     3241 cgctccgcgc tcctccgccg ttcgtcatgc ccaagggcgg ctggagtcga gccgccttcc
     3301 ggcggccgat tgccgttgaa gctagcgttc catgattacg gtgccccgct ggatgacgcg
     3361 gtcgagcacg atgacgacgg cacagccctt ccgctccgga tcgagactgc gctcgggctc
     3421 cggtgattcg tcgtagacgc gtttcgcttc gacgggatcc gtcgccacgt gagctcgacc
     3481 ctggaattgc cacccaaccc gcgcctcgaa gttctggtac atcagggcga cacgcggatt
     3541 cgacgctagg tggcgaagga acgcgccatc gggattgcgc gcccaaatcg cgagcgcatc
     3601 gtctccgtgc acgtgcgtgc tgcctcgaaa ggtcaggtgc ggctgcccct cttcgtccac
     3661 ggatgcgatg atgaggggtg cacgattcat cagcgcattg ttgatgtgct cgcgcatctc
     3721 ctccgtgagc ttgatcacca tgctcctccc caccaaaagg ttttccgcgc atgattgtaa
     3781 caggcgtcac acatcgaacg aacctcaggg ctgtggtagt gtgag
//
LOCUS       DATMBB010000145         2675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_482232, whole genome shotgun sequence.
ACCESSION   DATMBB010000145 DATMBB010000000
VERSION     DATMBB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482232"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VNL92_03090"
     CDS             complement(<1..460)
                     /locus_tag="VNL92_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWO93726.1"
                     /translation="MALMLLWLTVAWNVVEGAVAVTSGLVAQSLALVGFGLDSFIEVA
                     AAGILLWRLRLAEDGERAERREAIAHRVVGTTFLVLAGYILAEAAYTLTFGDPPEESR
                     VGLTLAVLSLIVMPALGLLKRANARRLGSRALVAESTETLICAYLSLTLFI"
     gene            complement(517..1455)
                     /locus_tag="VNL92_03095"
     CDS             complement(517..1455)
                     /locus_tag="VNL92_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWO93727.1"
                     /translation="MAHNRGSAGFQHKGRLLTVFALTAAFMVVEATVGFLAGSLVLIA
                     DAGHMLTDVAGLGMALGSIWLAQRRPSDRKTYGYYRAEILAALFNAVLLIAVAGYILY
                     EAFARLTDPPDVSAVPLIVVASGGLAVNLIGLALLHAGSKESLNVRGAFLEVWKDVLG
                     SIAAVAAGVIILTTGWTYADPILAAAVGLLILPRSWDLMKKTLEVLLEGTPEHIDLEE
                     LEKSMVAVPGVLAIHDLHVWTVTSGFVALSAHAETGERHDQHDTLVGLRRLLAERFGI
                     DHATLQIETRALHEELESCCGIDSRNTSPAHAAHHR"
     gene            complement(1458..1823)
                     /locus_tag="VNL92_03100"
     CDS             complement(1458..1823)
                     /locus_tag="VNL92_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWO93728.1"
                     /translation="MTTTEPAAGAWEVRHIDEDEVEAVRCSLLNREDAGQIADIFRVL
                     SDPTRVLILHALSLAELCNGDLAAILGISESAVSHQMRDLRLMKLVTAEKRGRMVYYR
                     LADSHIRHVFEDSLRHIKE"
     gene            2007..>2675
                     /locus_tag="VNL92_03105"
     CDS             2007..>2675
                     /locus_tag="VNL92_03105"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWO93729.1"
                     /translation="MAKLKLAPWFIVLGLIAGTLGPSVAAAGGPAEQEISIVAVSPPY
                     GSRDVDRLETLVVRFDREVRWSDTHLAIEPDTAIEVEQTAPNEIAISPRDGWLPGTSY
                     DVRILAGLTASDGAVLQQDFVFSFRTRAGTQLLAFEPQGEAVPTDSLVRVEFAQAPSD
                     PAAIAASLRVWRMAGAAPVAVDGGATWETPTTIVWRPATPFVAGADYIAAIGGPGTSA
                     ARHEW"
BASE COUNT          502 a          857 c          874 g          442 t
ORIGIN      
        1 cgatgaagag cgtcaaagac aggtacgcgc agattagggt ctcggtggac tccgcaacga
       61 gtgctcgcga gccgagccgg cgcgcgttcg ccctttttag aagtcccagg gcgggcatga
      121 ctatgaggga caggaccgcg agggtgaggc cgacgcgcga ttcttctggt gggtcgccga
      181 aggtgagggt gtaggcggcc tcagcaagaa tgtagccagc gaggaccagg aaagttgtcc
      241 caaccacacg gtgtgctatc gcttctctcc gctctgcgcg ctcgccgtcc tcggctaggc
      301 gaaggcgcca cagaaggatg ccggccgcgg cgacttcaat gaaactgtct aggccgaagc
      361 ctacgagcgc taggctctga gcgacaagtc cggacgtgac tgcgacggcg ccttcgacga
      421 cgttccaggc gacagtaagc cacagcagca tgagggccat gcgccgcgaa cccttgggac
      481 ccgctcgaac cgcgttctgt ggcctcacag ttctgcttac cgatgatgcg cagcgtgggc
      541 gggggacgta ttgcgggaat cgatcccgca gcatgattcg agctcctcgt ggagcgcacg
      601 cgtctctatc tgcaatgtgg cgtggtcgat gccgaatcgt tcggcgagca ggcgccggag
      661 gcccacgagc gtgtcgtgct ggtcgtggcg ctcaccggtc tcggcgtgcg cactgagcgc
      721 cacgaaaccg gacgtgactg tccagacgtg aaggtcgtgg atagcgagaa cgccgggcac
      781 tgcgaccatc gacttctcga gctcctcgag atcgatgtgc tccggcgtgc cctccaggag
      841 aacctcgagc gtcttcttca tcaggtccca cgagcgagga aggatgagaa ggcccacagc
      901 cgccgcgagg atcggatctg cgtaagtcca cccggtcgtg aggatgatga ccccggcggc
      961 aacggcggca atcgagccga ggacatcctt ccaaacttcg aggaaggcgc cgcggacgtt
     1021 caggctctcc ttggacccgg catggagcag agcgaggccg atgagattaa cggcgaggcc
     1081 gccactcgcc accacgatca agggaacggc cgacacatcg ggcgggtcgg taaggcgcgc
     1141 gaacgcctcg tagaggatgt agcccgcgac cgcgatgaga agcacagcat tgaagagggc
     1201 cgccagaatc tccgctcggt agtagccata agtcttccgg tcactgggcc gtcgctgggc
     1261 cagccagatg gaaccgaggg ccatccccag tccggcgacg tcggtgagca tatgccccgc
     1321 gtccgcgatg aggacgaggc tgccggccag gaaacccacc gtcgcctcga cgaccatgaa
     1381 tgccgccgta agggcgaaga cggtgagcag gcgacccttg tgctggaacc cggcgctccc
     1441 gcgattgtga gccaaggcta ctccttgatg tgccgcagag agtcctcgaa gacgtgccgg
     1501 atatgggagt ctgcgagacg gtagtagacc atccggcccc gcttttccgc cgtgaccagc
     1561 ttcatcagac gaaggtcgcg catctgatgg gagactgcgg actcagagat gccgaggatc
     1621 gcagccaggt cgccgttgca gagctcggcc agtgacaggg cgtggaggat gaggacgcga
     1681 gtagggtccg ataggacgcg gaagatatcc gcaatctggc cagcatcttc gcggttgagg
     1741 agggagcaac gaaccgcttc cacctcatcc tcatcgatgt ggcgaacttc ccatgctccc
     1801 gcggctggtt ccgtggtcgt cacgcaggca ccatacttga acagttgctc atctgtcaat
     1861 ccggatcctt ctggtgccaa agctcagcgc ccgctatcat acggcgagcg ccccccgggc
     1921 gaccttcgta tcggtctcgc ctccatcgtg gaacttacga gcgtgctgcg cccaagcacc
     1981 cgctcgcccc agaaggaagg accagcgtgg ccaagctcaa acttgcgcca tggttcatcg
     2041 tgcttggcct catcgcgggc acgctcgggc caagcgtcgc ggccgccgga ggccctgccg
     2101 agcaggagat cagcattgtc gccgtgtcgc ctccgtacgg ctcccgcgac gtcgaccgcc
     2161 tcgaaacgct cgtggtccga ttcgatcggg aggtccgctg gagcgacacg catctcgcca
     2221 tcgaaccgga taccgcaatc gaggtcgagc agacggcgcc caacgaaatc gccatctctc
     2281 cccgggatgg ctggcttccg gggacctcct acgacgtgcg cattcttgcc ggcctgacgg
     2341 catcggatgg agccgtgttg cagcaggact tcgtcttctc gtttcgaaca cgcgcgggca
     2401 cccaactcct cgccttcgaa ccgcagggcg aagccgtccc gacggactcc ctcgtgcgcg
     2461 tcgagttcgc ccaggctccc tccgacccag cggccatcgc cgcatccctt cgcgtttggc
     2521 gcatggccgg cgcggcaccg gtagcggtgg acggaggcgc cacgtgggaa acgccgacca
     2581 caattgtgtg gcggcccgcg acgccatttg tcgccggggc cgactacatc gcggctattg
     2641 gcggtcccgg tacgtccgca gcccgacacg agtgg
//
LOCUS       DATMBB010000146         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_4526, whole genome shotgun sequence.
ACCESSION   DATMBB010000146 DATMBB010000000
VERSION     DATMBB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4526"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1303
                     /locus_tag="VNL92_03110"
     CDS             134..1303
                     /locus_tag="VNL92_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWO93730.1"
                     /translation="MTDPWTAMIVGLLVFLATIVSVEIGISVALIEITIGVIAGNFLG
                     LQTTPWIDYLAAFGSIVLTFLAGAEVDTDLLREQLKGSLWIGGLSFTLPFLAAWGVAL
                     WVLGWNPAASKIAGIALSTTSLAIVYAVLVETGLTGTPLGKLIMAGTFVTDFGTAAAL
                     SVLFVRPTVWIVVFLGVSTAIIVLMPRLQPWFFRRYGSRVIEPEIKGAFAALLVLIYF
                     GALADSHAVLPAFVLGLALSRVFAAHRAEQRRFRVIAFAMLTPFFFLKAGMNVELGVV
                     AANLGVLTLLLGVKLAAKFAGVLPAAQRYVGAHAMYTTLLMSTGLTFGTISSLYGLQA
                     GIIDQTQFSLLVTTVILSAVLPTVVAQRLFDPRRRGVPVPQAARVLPRVPAFEEE"
     gene            1310..1744
                     /locus_tag="VNL92_03115"
     CDS             1310..1744
                     /locus_tag="VNL92_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO93731.1"
                     /translation="MIAHILIANDGSEEARRAVDVGTDLALRYGAKATVLTVIYHPAF
                     AATVGEVQEADAEQRAVAHRVQREAIEFARMRGLDIGAAIVSGHPAEAIVDYAHEHDV
                     DLIVMGHRGMSNLRRFLVGSVADRVVDHAPCMVLVVRRDPRP"
     gene            complement(2365..>3442)
                     /locus_tag="VNL92_03120"
     CDS             complement(2365..>3442)
                     /locus_tag="VNL92_03120"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HWO93732.1"
                     /translation="ALPLIVGMILAAGLDSASVLVMFGATQIATGIWYRMPMPVQPLK
                     AMATIVITQKLTGNVLYGGGLAIGVLMLVLTGTRLVEWLARITPVVVIRGIQFGLGLQ
                     LATLALREYVRAEGVPGYVLAAVAFLATLALMGNRRYPAALAVIILGIVYAALFTLRT
                     DALLAGVGLRWPRLFVPTMSDALTGLLVLALPQLPLSLGNSILATQQSARDFFPGRPL
                     TVRKISATYAAMNLIQPFFSGVPVCHGSGGLVGHYTFGGRTGGSVIIYGSLYVLLGLF
                     LSEGFDAAIRIFPLPVLGVLLLFEGLALMTLLARLPNLEMDLAVALLVGVIAGSVPYG
                     YVVAMALGMLLASPVGRARVRLAR"
BASE COUNT          594 a         1126 c         1141 g          581 t
ORIGIN      
        1 cagcaggagc gtttcgtttc agcaccgggc tgggtagtcg gatgagcggc cgtcctgaga
       61 tagcagaggc ggccgacacc gtggccggac gggcgccata cgctggcgaa aacgtgcgtt
      121 cgaaggggaa gcgatgaccg atccgtggac cgccatgatc gtggggctgc tcgtgttcct
      181 cgcgacgatt gtgtccgtcg agatcggtat ctcggtggct ctcatcgaga ttacgatcgg
      241 tgtcatcgcc gggaacttcc tcgggctcca gaccactccc tggatcgatt acctggccgc
      301 gttcggcagc atcgtcctga ccttcctcgc tggcgccgag gtggacaccg atctccttcg
      361 ggagcaactg aaggggagcc tctggatcgg cgggctgtcg ttcacccttc cctttctggc
      421 ggcgtggggg gttgcgctgt gggtgttggg ttggaatccg gcggcctcga agatcgccgg
      481 catcgcgctc tccacgacgt cgctcgcgat cgtgtacgcg gtgttggtcg agacgggcct
      541 gacgggcacg ccgctgggca aactgatcat ggccggcacc ttcgtcaccg acttcggcac
      601 ggcagcggcg ttgagcgtgc ttttcgtccg accaaccgtg tggatcgtcg tgtttctcgg
      661 cgtatcgacc gccatcatcg tcctcatgcc tcgtctgcag ccgtggttct tccggcgcta
      721 cgggagtcgt gtgatcgaac ctgagatcaa gggagcgttc gccgccctgc tggtcctgat
      781 ctacttcggt gcgctcgccg acagccacgc cgtcctgcca gcgttcgtgc tggggctggc
      841 cctgtcaagg gtcttcgccg cccaccgcgc cgagcagcgc cggtttcgtg tgatcgcgtt
      901 cgcgatgctg acgcccttct tcttcctcaa ggccggaatg aacgtggagc tgggcgtcgt
      961 ggccgcgaac ctgggggtgc tcaccctgct gctcggggtc aagctcgcgg cgaagttcgc
     1021 gggcgtcctg cccgccgcgc aacgctacgt cggggcccac gccatgtaca cgacgctcct
     1081 catgagcacc ggcctgacgt tcggtaccat ctcgtcgctc tacgggttgc aagccggtat
     1141 catcgaccag acacagttct ccctgttggt gacgacggtc atcctgagcg ccgtgctgcc
     1201 gacggtggtc gcacagcggc tcttcgaccc ccgtcgccgc ggcgtgccgg tgccgcaggc
     1261 ggcgcgtgtt ctcccgaggg tgccggcctt cgaagaggag tgaggatgga tgatcgccca
     1321 cattctgatc gccaacgacg gctcggagga ggcgcggcga gcggtggacg tgggaacgga
     1381 tctggcgctg cggtatggcg cgaaggcgac ggtgctgacg gtgatttatc acccggcgtt
     1441 cgcggccacc gtcggggaag tccaggaagc cgacgcggag cagcgggcgg tggcacaccg
     1501 ggtccagcgc gaagccatcg agttcgcccg catgcgcggc ctcgacatcg gcgcggccat
     1561 tgtctctgga catccggccg aggccatcgt cgactatgcg cacgagcacg acgtggacct
     1621 gatcgtgatg ggccaccgag gcatgtccaa cctgcggcgc ttcctggtcg gcagcgtcgc
     1681 cgaccgcgtc gtcgatcacg cgccgtgcat ggtcctggtg gtcaggcggg acccgcggcc
     1741 ttagacggcg ctcgggcgac ggcccgagca gggaccgcct caaccgctcg cggcgagccc
     1801 cttgccgggc gagggaagcg cgaaactgga ggttgctctt ctccagtccc gtgatgttga
     1861 gcctcaaggt acgcatcaca tcttctgcaa gatacgcacg gtgcctcagg acggcgagac
     1921 tcgccgcggg ccgccgacca ctcgtcgcgt acctcctggg ctcgtcgtcg cggcactgcg
     1981 aggtccgcgg cgtaggcgag caggatcttg gccccctcct ccggcgtccg ccccttggtc
     2041 cgaacaaacg ctttgaagcg ctcggcgagg ggttctcgaa gagacagcac aacccgttgt
     2101 agcaagcgtc cgccacggcc tcaaccgcgc gccagacggg ttcccagtcc tcggcgagca
     2161 gctcgatctc gatctccatg ccgcactcga tccttccatc ggcgcctgta aagcgaagag
     2221 ctcctaccga ccggatccgt aggagctatc agcccacgcg ggcgcctgaa cgagctccat
     2281 cgcctatgac gttccactcc cactgcgatc ttatggggca aatggacgat gtcaacccac
     2341 acgggcgtga tcctgttccc agcttcaacg ggccagccgc acacgtgcgc gccccaccgg
     2401 agaggccagg agcattccca gggccatggc cacgacgtat ccatacggta cgcttccggc
     2461 gatcaccccg acgagcaggg cgacggcgag atccatctcg aggttcggaa ggcgtgccag
     2521 aagcgtcatg agcgcgagcc cctcgaacag gagcagtacc cccaacacgg gcaagggaaa
     2581 gatgcgaatg gccgcatcga agccttcgct gagaaagagt cccagaagca cgtagaggct
     2641 tccgtagatg atcaccgagc cgcctgtgcg tcctccgaac gtatagtggc ccaccagccc
     2701 gcccgaaccg tggcagacag ggacgccgct gaagaacggc tggatgagat tcatggccgc
     2761 gtaggttgcg ctgatcttcc gcacggtcaa cggacggcct ggaaagaagt ctcgggcgct
     2821 ctgttgagtg gcgagaatcg agtttcccag cgagagcggc agctgcggca gcgcgagcac
     2881 caacagcccg gtgagagcgt ccgacatcgt cggtacaaag aggcggggcc atcgcaggcc
     2941 gacgccggcc agcagggcat ccgtgcgcag cgtgaagagc gcagcataga cgatgcccag
     3001 aataatgaca gcgagcgcag cggggtaccg gcgattcccc atcagcgcca gggtggcgag
     3061 gaaggccacc gcggccaaca cgtacccggg aactccctcg gcccgcacat actcgcgcag
     3121 ggcgagtgtc gcgagctgca gccccaatcc gaactggatg ccgcgaatga cgacgacggg
     3181 cgtgatccgc gccagccact cgacgaggcg cgtgccggtc agcaccagca tgaggacgcc
     3241 aatggccagg ccgccgccat agagcacgtt gccggtgagc ttctgggtga tgacgatggt
     3301 cgccatcgcc ttcagcggtt gcaccggcat gggcatccgg taccagatac cggtcgcgat
     3361 ctgtgtcgcc ccgaacatga ccaggacact agcactgtcc agaccggcgg caaggatcat
     3421 gccgacaatg agcggcaggg cc
//
LOCUS       DATMBB010000147         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_422251, whole genome shotgun sequence.
ACCESSION   DATMBB010000147 DATMBB010000000
VERSION     DATMBB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422251"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..572)
                     /locus_tag="VNL92_03125"
     CDS             complement(87..572)
                     /locus_tag="VNL92_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93733.1"
                     /translation="MVDERDRRVPTTDTQRAIAATGVRHLDLAEARIQVHRALGRALP
                     GRERDWADHVAGTLELLRTALADHKGDALGAKGLYEQIRFDEPRLTRRVDELEAELLR
                     IEGVAAQLHERAQRISQGERAGIETMRDDTEALLKAIRDLLAAEADLVWEQYNEPIAL
                     D"
     gene            730..1629
                     /locus_tag="VNL92_03130"
     CDS             730..1629
                     /locus_tag="VNL92_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO93734.1"
                     /translation="MTYTVLVPFDGSPTAHRAIPYAAALAKLHPSPRLVLLTVFVPTL
                     YGQIDGWQPDAETERAATRELEQVAALAREAGVPDIAVRTATGVTEAREILRAAEDIG
                     ADAIALGTHGRGGLSRALLGSVAMAVATRSSIPCLLVPPGSPVGHAEIARAVLTTDGS
                     DAAAAAIPVAQDLARHGVHVDVVEVVDPSAKLLAFTIPAADEGFVPQRIMRELLPPAR
                     ARVRQIAGQFPSGTADGVVVRGRASAAITRYVHTHDIDLIVMASHGRTGASRLWWGSV
                     ADRVIRSARVPVLLVRSSDQGTA"
     gene            complement(1696..>2241)
                     /locus_tag="VNL92_03135"
     CDS             complement(1696..>2241)
                     /locus_tag="VNL92_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWO93735.1"
                     /translation="RFIVVLSLVLAFAWAAEEVGGLAAISGAYLVGVLLGRTVMKEQL
                     TEFGNLIGYAFFAPVFFSITGMSVDLGALAEAPVFTMLLVAVAVVTKAAGCYAGSLAG
                     GFSHGDSLSVGLGMVARGEVALVMAVLGREEGLLNDEAFAAAIAMTLLTTLVTPLVLP
                     LALRSTAPSTSASREPAAQAT"
BASE COUNT          354 a          793 c          733 g          361 t
ORIGIN      
        1 ctgctcgccc gccggcgata ctatcgctga cgttcggccc ccgcgaccat tgggagggac
       61 gccttggggc gtccctcccg tgccgctcaa tccagggcga taggctcgtt gtattgctcc
      121 cacacgaggt cggcctcggc cgctagcagg tcgcggatgg ccttgagcag cgcctcggta
      181 tcgtcacgca tcgtctcgat gcccgcacgc tcgccttggg atatgcgttg ggctcgctcg
      241 tgcagttgcg ctgccacgcc ctcgattcgg agcagctcgg cttccagctc gtcgacgcgg
      301 cgcgtcaatc gcggctcgtc gaatcgaatc tgctcgtaga gtcccttcgc accaagggcg
      361 tcgcccttgt ggtcggcgag agccgttcgc agaagctcga gcgtcccggc gacgtgatct
      421 gcccaatcgc gttccctccc ggggagggct cgtcccaggg cgcggtggac ctgaatgcgc
      481 gcctcggcga ggtcgaggtg tcgaacgccc gtcgcggcga tcgcgcgttg cgtgtcggtg
      541 gtcggcacgc ggcggtcccg ttcatcgacc atggcacgcc tccaaaatca attttgtgcc
      601 tctagcatgc catggaaagg acgccgtacg ggagagtcgc taagccctcg aaagaagggc
      661 cgaccggccg gttgccccgc gctatgctgg tgacgtacaa gtgcagtgga caactctgga
      721 gacgatgcaa tgacgtatac cgtgctcgtt ccgttcgacg gctcccctac cgcccatcga
      781 gcgataccgt atgcggccgc gctcgccaag ctgcatcctt cgccgcgact cgtgctgctc
      841 acggtgttcg tccccacgct gtacgggcag atcgatggct ggcagccaga cgccgagacc
      901 gagcgcgctg cgacgcgtga gcttgagcag gtcgcggcgc tcgcacggga agccggcgtg
      961 ccggacatcg cggtgcgcac ggcgaccggg gtcacggagg cacgcgagat ccttcgcgcg
     1021 gccgaggaca tcggcgccga tgccatcgcc ttgggcacgc acgggcgcgg cggcctgtct
     1081 cgcgccctgc tcggaagcgt ggccatggcc gtcgctaccc gcagctccat accttgcctc
     1141 ctggtgccgc ccggctcccc ggttgggcac gcggagattg cgcgggccgt ccttaccacc
     1201 gacggctctg acgccgcggc cgcggccatc cccgttgccc aagacctcgc gcgccacggc
     1261 gtgcacgtcg acgtggtcga ggtggtcgat ccgtccgcga aactcctcgc cttcaccata
     1321 cccgcggcgg acgagggatt cgtgccccaa cggatcatgc gcgagcttct gccgcccgcc
     1381 cgggctcggg tgcggcagat cgcaggtcag ttcccatccg gaaccgccga cggcgtggtc
     1441 gtgcgcggac gggcgagcgc cgccatcacg cgctacgtcc atacgcacga tatcgacctt
     1501 atcgtcatgg cctcgcacgg aagaacgggg gccagccgtc tttggtgggg gagcgtcgcc
     1561 gaccgcgtga ttcgctctgc gcgcgtgccc gtcctgctcg ttcggagcag cgaccagggg
     1621 acggcgtagc ccgcacaggt ggtccttcga tccgacacac caccgcgagc gccccaagcc
     1681 gaggcgcccc cagcgttatg tggcctgtgc cgcaggctct ctgctggcgc tcgtcgatgg
     1741 cgccgtggaa cgcaaggcga gggggagcac gagcggcgtc acgagtgttg tgagcagggt
     1801 catggcgatg gcggcggcga acgcctcgtc gttgaggagc ccttcctccc tcccgagcac
     1861 ggccataacc aacgccactt cgccgcgcgc aaccatcccg agtcccacgc tcagcgaatc
     1921 gccatgcgag aatcctcccg cgagcgaccc cgcatagcag cccgctgcct tcgtcactac
     1981 cgcgaccgca accagcaaca tcgtgaacac cggtgcctcg gcgagcgccc ccaagtccac
     2041 gctcatgccg gtgatggaga agaatacggg agcgaagaac gcatagccga tgagattgcc
     2101 gaactcggtg agctgctcct tcatcacggt gcggccgagc aacacgccca cgaggtacgc
     2161 gcccgagatg gcagcgagtc cgccgacctc ctctgccgcc cacgcgaagg cgaggaccag
     2221 tgacagcacg acgatgaacc g
//
LOCUS       DATMBB010000148         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_47019, whole genome shotgun sequence.
ACCESSION   DATMBB010000148 DATMBB010000000
VERSION     DATMBB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47019"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VNL92_03140"
     CDS             <1..741
                     /locus_tag="VNL92_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWO93736.1"
                     /translation="HQAALRITQCAEMPRLAVDSLIGGTFNVLEAAVEAGVRKVVAAS
                     SASVYGEASYVPMDEAHPFNNRTLYGAGKVSLEQLLRSFNDMYGLPYLALRYFNVYGP
                     RMDAYGVYTEVMIRWLQRLQQGERPIIFGDGKQTMDFVFVEDVAEANVLAMQSEASDE
                     ALNVASGVETSLAELCRLMCDEAGRPDLEPEHQPARKVNPVTRRLASTRRAQERIGFV
                     ARTPLRAGLRRLVEWHAALPAAEEVAAR"
     gene            738..1865
                     /locus_tag="VNL92_03145"
     CDS             738..1865
                     /locus_tag="VNL92_03145"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWO93737.1"
                     /translation="MIPITRPSVGPEEAQAAAEAVLSGWLSQGQRVREFEQAVATYVG
                     ARHAIATTNCTSALHLSLLAAGIGPGDEVICPSFSFIATANSIVHAGATPVFVDIDPR
                     TYTIDPDLIEAAVTERTRAIMPVDQIGLAADISAVLEVARRHGLAVIEDAAPSLGATV
                     GEARVGGLADFTCFSFHPRKSITTGEGGMIVTNDDGAAEFLRRTRSHGASVSDLSRHV
                     SGTVDIEEYHELGYNFRMTDIQAAIGLVQMRRLDDVLGARRRLAQRYNALLSGLDMFE
                     TPFEPEGRRHTYQSYCIRLRTQRPRHEIMAELASEGIATRRGVMAIHLEPYYRRRFPG
                     IHLPETERAAAETLLLPLYYGMTDAEQDLVVEKLIDAARGG"
     gene            1877..2884
                     /locus_tag="VNL92_03150"
     CDS             1877..2884
                     /locus_tag="VNL92_03150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO93738.1"
                     /translation="MQNDNEMSNLQVRWFDEWHPELDEALRLLPEMETCPHELFRMLM
                     VNRTSNRKRTALVTARGEPLAVVGLRQKRHHWELVPPDASPEAFLPAAPEAFYPTLAA
                     LGVDLWVWGLAEGAPPPGVARFAQAEPTYKVDCQADYEEHWRETGHIRVVRSARERTQ
                     GYRLEIDAPGAAEWTIRNWAAKWAARGSDTLSRAEDQIVAASYLQPRGQLHTFRLFDD
                     DEPVAGATLVVAGAELIGMSIYSSDAYRKQAVGTRLMDAIFAWAREAGYRKVELGTGD
                     GYKAGIAPADGCRWTLNFCPGPAYAFKRTVRWMASPLATARMRIAAIAPARIRVQQEA
                     G"
     gene            2881..>3236
                     /locus_tag="VNL92_03155"
     CDS             2881..>3236
                     /locus_tag="VNL92_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93739.1"
                     /translation="MISPTNESPDRRLRVRWFDAWHPELDKALATLPEMPTCPHELFR
                     MLMQNPIGARKQTALVTEDGRPVAVVGLRRKHRHWEPVPQDPSPRAVAPAAPGFLVPA
                     LRALDRDIWVWGFDDF"
BASE COUNT          547 a         1090 c         1059 g          540 t
ORIGIN      
        1 catcaggccg cgcttcgcat cacgcagtgc gcggagatgc ctcgcctcgc ggtcgatagc
       61 ctcatcggcg gcacattcaa tgtcctcgaa gctgcggtcg aagccggcgt tcggaaggtc
      121 gtcgctgcct cgagcgcgtc cgtctatggc gaggcttcct acgtcccgat ggatgaggcg
      181 cacccgttca acaatcgcac gctctacggc gccggcaagg tatcgctcga gcagttgctc
      241 cgcagcttca acgacatgta cggcctccct tacctcgcgc tgcgctactt caacgtctat
      301 ggacccagga tggacgcgta cggcgtgtac accgaggtga tgatccggtg gctccagcgc
      361 ctccagcaag gcgagcgtcc catcatcttc ggtgacggca agcagaccat ggacttcgtc
      421 ttcgtggagg acgtcgccga ggccaacgtc ctcgcgatgc agtccgaagc gagcgacgag
      481 gcgttgaacg tcgcgtcggg cgttgagacg agcctggcgg agctgtgccg cctgatgtgc
      541 gacgaggcgg gccggcccga cctcgagccc gagcatcagc ccgcacgcaa ggtgaacccg
      601 gtgacgcgaa ggcttgcctc cacgcgccgc gctcaggagc gaatcggctt cgttgcgcga
      661 acgccgctcc gcgctgggct gcggaggctg gttgagtggc acgccgcgct gccagccgcg
      721 gaggaggtcg ctgctcgatg attcccatca cgcgacccag cgtaggcccg gaggaggcgc
      781 aggccgccgc cgaagccgta ctctcgggct ggctctccca agggcagcgc gtgcgcgagt
      841 tcgagcaggc cgtcgcgacg tatgtcggcg ctcgccacgc catcgccaca acgaactgca
      901 ccagcgcgct gcacctctcg ctccttgcgg ccggaattgg ccccggcgac gaggtcatct
      961 gcccgtcatt cagcttcatc gcaactgcga actccatcgt gcacgctgga gcgacgcccg
     1021 tcttcgtgga catcgacccg cggacgtaca cgatcgatcc ggatctcatc gaagcagcgg
     1081 tcactgaacg gacccgcgcc attatgcccg ttgaccagat cggcctcgcg gcggatatct
     1141 ccgctgttct cgaagtcgcg cggcgccacg gcctcgcggt catcgaggac gccgctccgt
     1201 cgctcggcgc cactgttggc gaggcgcgcg tgggcggcct ggccgacttc acctgcttta
     1261 gcttccatcc acgcaagagc attacgacgg gcgaaggcgg aatgattgtc acgaacgacg
     1321 acggcgctgc cgagttcctg cgacgtacgc gctctcacgg cgcgtccgtc tccgatctct
     1381 cgcggcacgt ctcggggacc gtcgacatcg aggagtacca cgagcttggc tacaacttcc
     1441 ggatgaccga catccaggcg gccatcggcc tcgtccagat gcgtcgcctc gacgatgtcc
     1501 tcggtgcgcg ccgccgcctg gcgcagcggt acaacgccct gctctccggc ctggatatgt
     1561 tcgagactcc cttcgagccg gaggggcgac gccataccta tcagtcgtac tgcatccgcc
     1621 ttcgaaccca gcgcccgcgc cacgagatca tggccgagct cgccagcgaa ggcatcgcga
     1681 cgcgacgggg tgtcatggcc atccacctgg agccctacta ccgacgacgg tttcctggta
     1741 ttcacctgcc ggagacggag cgtgccgcag ccgagacgct gttgctcccc ctctactacg
     1801 gcatgactga cgcggagcag gacctcgtcg tcgagaagct gatcgatgct gcccgcggcg
     1861 gctgaggcaa ggagtcatgc agaacgataa cgagatgtcc aatctgcaag tacgctggtt
     1921 cgacgaatgg cacccggagc tcgatgaggc gctccgcctt cttccggaga tggagacgtg
     1981 cccgcacgag ctgttccgga tgctcatggt gaaccgtact tcgaatcgca agcggacggc
     2041 cctggtcacg gcacggggcg agcccttggc ggtggtgggc ctgcgccaaa agcgccacca
     2101 ttgggagctc gttccccccg atgcttcgcc ggaggccttt ctccctgcgg cgccagaagc
     2161 gttctatccg accctcgcgg cgctcggcgt cgacctgtgg gtctggggct tggcggaagg
     2221 ggcgccgccc ccaggcgttg cgcgcttcgc tcaggccgag cccacgtaca aggtcgattg
     2281 tcaggcggac tacgaggagc attggcgcga aacgggacac atccgtgtgg tgaggtcggc
     2341 gcgcgagcgc acccaaggct atcgcctgga gatcgatgcg cccggcgcgg cggaatggac
     2401 gattcggaac tgggccgcga agtgggccgc ccgcgggagc gacacgctgt cgcgcgccga
     2461 agaccagatc gtcgccgcgt cctatctgca accacggggc cagcttcata catttcggct
     2521 gttcgacgat gatgagccgg tcgctggcgc cacactcgtc gtcgccggtg ccgagctcat
     2581 cggcatgtcc atctacagca gcgacgccta ccgaaagcag gccgtaggaa cgcggctgat
     2641 ggacgcgatc tttgcctggg ccagggaagc cggctaccgg aaggtggagc ttgggaccgg
     2701 cgacggctac aaggcgggga tcgcgcctgc ggacggatgc cgttggaccc tcaacttctg
     2761 tccgggcccg gcctacgcgt tcaagcggac tgttcgatgg atggcatccc cactggcaac
     2821 ggcgaggatg cgaatcgcgg cgatagctcc cgcccgtatc cgggtgcaac aggaggcagg
     2881 atgataagcc caaccaacga atcgcccgac cggcgtctgc gcgtccgctg gttcgatgca
     2941 tggcatccgg agctcgacaa ggcgctggcg acgctgcccg aaatgccaac atgcccgcac
     3001 gagctgttcc gaatgctgat gcagaaccct atcggcgcgc gaaagcagac ggcgctcgtc
     3061 acggaggatg gccgccctgt cgccgtcgtg ggcctacggc ggaagcatcg tcattgggag
     3121 cccgtgcccc aggatccgag tcctcgagcc gtcgcgccgg cagcgccggg cttccttgtg
     3181 ccggccttac gcgcgctcga ccgcgacatc tgggtttggg gcttcgacga cttcga
//
LOCUS       DATMBB010000149         3358 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_588265, whole genome shotgun sequence.
ACCESSION   DATMBB010000149 DATMBB010000000
VERSION     DATMBB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588265"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VNL92_03160"
     CDS             complement(<1..1305)
                     /locus_tag="VNL92_03160"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HWO93740.1"
                     /translation="MTAPLDESQAELVAVGYFRDLGYDYAFGPDIAPDGERPERGDYA
                     QVVLLGRLRAALRRINPDVPGEAIEDAIRKVTRPESPSLVANNRAFHRLLVEGVDVSW
                     MEDGRERHGKVWLVQRNPARLDDNEFLVVNQFTVIEDRRNRRPDVVVFLNGLPVSVIE
                     LKNPADETTTIRHAFNQIQTYKADIPSLFTFNEIVAISDGFAAKLGTLTAGWDRFSPW
                     RTVDGDAIASAGTVQLEVLTRGVFEKRRLLDYLLHFITFEDDGREVVKKAAAYHQYWA
                     VNKAVECTVAATRPDGDKRAGVVWHTQGSGKSLSMVFYAGKIIAHAAMENPTIVVITD
                     RNDLDDQLFGTFTVNSDLLRQKPIQAESREHLKTLLRVAAGGVVFTTMQKFEGGDSLS
                     DRRNIVVIADEAHRSHYGFKAKIDQKSGDIKYGMAKYLRDALP"
     gene            complement(1302..>3358)
                     /locus_tag="VNL92_03165"
     CDS             complement(1302..>3358)
                     /locus_tag="VNL92_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO93741.1"
                     /translation="EDFPQSTLPIRVFNRDFIHESVFPVGGGDVPPIFVVGKESVEKQ
                     KEADRLKTERSGKQEQLDAAQRAKGQADRDFDKHCIDRAKVIKDTLQKTGSAYNNCNK
                     TDYQGRAQRMTADGDAASRRLSDDQREALLAQHQATPKPKVSAVTYQVPALQGLADQV
                     AALLRTTVVSSAIQALKDDSALAEWVRHGLGLHKDRRPDKCLFCEQPLPAGRLGELEA
                     HFNAEYERLVQRIDDQIRALEAANGQAAGVRSPNRAELYDDLRGDFDSAERAFRQAID
                     AVRTYLAVLVENLKRKRGEPFAALSLSEAVPTVDTAAIDRLNEVVRRHNQACDEFDRR
                     VSDARDRLALDMIAQALDEFVRLRDAAQAAVAAIGPIEREIKRLTEEIERLEREIREH
                     RRPAEELNEDLKRYLGHGDLQLTIKDNGYSITRNGVPADMLSEGEMSAIALLYFLKSL
                     EDRGFDRPNGVVVLDDPVSSLDGNSLFLAFGLIRERTKDAGQLILMTHDFALFRQARN
                     WLRHLPGQRKREVSERPARFYMLVCASQDGQRATTLRWVDPLLERFESEYHYLFAYTY
                     RIWSAPPAASLEDYYHVPNVSRRVLEWFLAFKVPQASGELHSALQHVAFDESKKTRII
                     RFMHTQSHGADLVPGHDPAALGECRAVLEDLFALMRALDEGHFSAMVQLTNPPADGAN
                     GS"
BASE COUNT          561 a         1122 c         1021 g          654 t
ORIGIN      
        1 cgggagcgca tcgcgcaggt acttcgccat cccgtacttg atgtcgcccg acttctggtc
       61 gatcttcgcc ttgaacccgt agtgactcct gtgcgcctcg tcggcgatca ccacgatgtt
      121 ccggcgatcg ctcagcgagt cgcccccctc gaacttctgc atcgtcgtga acacgactcc
      181 gccggcggcc actcgcagca gtgtcttcag gtgctcgcgg ctttcggcct ggattggctt
      241 ctgccgcagc aggtcgctgt tcacggtgaa cgtgccgaac agttggtcgt cgaggtcgtt
      301 gcggtcggtg atgacgacga tcgtcgggtt ctccatcgcc gcatgcgcga tgatcttccc
      361 ggcgtagaag accatcgaca ggctcttgcc ggacccctgc gtgtgccaca cgacgcccgc
      421 acgcttgtcc ccatcgggcc gagtcgcggc cacagtgcac tcgaccgcct tgttgacggc
      481 ccagtactgg tggtacgccg cggccttctt caccacctcc cgcccatcat cctcaaaagt
      541 gatgaagtgc agcagatagt ccagcagccg gcgcttctcg aatacgcctc tggtcaagac
      601 ctccagctga accgtgccgg cagacgcgat ggcgtcgcca tcgaccgtcc gccacggcga
      661 gaaacggtcc caccccgcgg tcagcgtccc caacttcgcg gcgaagccgt ccgagatggc
      721 cacgatctca ttgaaggtga agaggctcgg gatgtccgcc ttgtaggtct gaatctggtt
      781 gaaggcatgc cggatggtcg tggtctcgtc ggcggggttc ttcagttcga tcactgacac
      841 gggcaggccg ttcaggaaga ccacaacgtc cggcctgcgg ttccgccgat cctcaatgac
      901 cgtgaactga ttgacgacga ggaactcgtt gtcgtcgagt cgggcagggt ttcgctgcac
      961 caaccatacc ttcccgtgcc tctcgcgccc gtcctccatc caggagacgt ccaccccttc
     1021 gaccagcagc cgatggaacg cgcggttgtt ggcgaccaga gacgggctct ccgggcgcgt
     1081 gaccttgcgg atggcgtcct cgatcgcctc ccccggcacg tcggggttga tccgccgcaa
     1141 cgcggcccgc aaccgcccga gcagaacgac ctgcgcgtag tcgccccgct cggggcgctc
     1201 gccgtccggc gcgatgtccg ggccgaaggc gtagtcgtac cccaggtccc ggaagtaccc
     1261 caccgcgaca agctcggcct gggattcatc gagtggggca gtcatgaccc attggctcca
     1321 tctgccggtg gattcgtcaa ctggaccatc gccgagaagt gtccctcatc gagcgcgcgc
     1381 atcagagcga acagatcctc cagcacagct cggcattccc ctaacgcggc cggatcatgc
     1441 ccgggcacaa gatcggcacc gtgcgattgc gtgtgcatga agcggatgat tcgggtcttc
     1501 ttgctctcgt caaatgctac atgttgcagc gcgctgtgca gttcacctga agcctgaggg
     1561 accttgaagg ccaagaacca ctcaagcact cgccgcgata cgtttggaac gtggtaataa
     1621 tcctccagac tcgccgcggg aggggcggac catatgcggt aggtgtaggc gaagaggtag
     1681 tggtactccg attcgaagcg ttcgagcagc ggatcgaccc accggagagt ggttgcacgc
     1741 tggccatctt gggaggcgca cacgagcatg tagaaacgcg cggggcgctc gctcacctcc
     1801 cgcttgcgct gacccgggag gtggcgcagc cagttccgcg cctgcctgaa tagcgcgaag
     1861 tcatgcgtca tcaggatgag ttgcccggca tccttggtcc gctctcgaat cagcccgaag
     1921 gccagaaaca gggagttgcc gtcgaggctt gacacgggat cgtccaggac gaccacgcca
     1981 ttgggcctat caaacccacg atcctccagc gacttcagga agtagagcag cgcgatcgcc
     2041 gacatctcac cctcgctgag catgtcggcg gggacgccgt tccgggtgat gctgtagccg
     2101 ttgtccttga tggtcagttg caagtcgccg tgtccgaggt accgcttcag gtcttcgttc
     2161 agttcctcgg cggggcggcg gtgctccctg atctcgcgct cgagccgctc gatctcctcc
     2221 gtcaggcgct tgatctcccg ttcgatcggg ccaatcgctg caacagccgc ttgagcagca
     2281 tcccggagcc gcacgaactc gtccagggcc tgcgcgatca tgtccagagc caggcgatcg
     2341 cgtgcatcgc tcacgcggcg gtcgaactcg tcgcacgcct gattgtgccg ccggacgacc
     2401 tcgttcagcc tgtcgatggc cgccgtatca acagtcggca cagcctcgct tagagacagg
     2461 gccgcgaacg gctcgcccct cttgcgcttc aggttctcca cgagtacggc gaggtacgtt
     2521 cgcacggcat cgatcgcctg ccggaacgct cgttcggcgg agtcaaagtc ccctctcaga
     2581 tcatcgtaca gttcagcccg attgggagac ctcaccccgg ctgcctgccc gttggctgct
     2641 tccaacgccc ggatctgatc atcaatccgc tgcacaagcc gttcgtactc ggcgttgaaa
     2701 tgcgcctcca gttcaccgag gcggcccgcg ggcagtggtt gctcgcagaa gaggcacttg
     2761 tccggtcggc ggtccttgtg caaaccgagc ccatggcgga cccactccgc aagcgcagag
     2821 tcgtccttca gggcttggat ggccgacgag acgacggttg tgcgcagcag cgcggcaacc
     2881 tgatcagcca gcccctgaag ggcgggaacc tggtaggtga ctgccgacac ctttggcttg
     2941 ggggtggcct ggtgttgcgc aagcaatgcc tcgcgctggt catcgctcaa tcgacgcgag
     3001 gcggcgtctc catccgccgt catccgctgc gcccggccct gatagtccgt cttgttgcag
     3061 ttgttgtacg cgctgccggt cttctggagt gtgtccttga tgaccttggc ccggtcgatg
     3121 cagtgcttgt caaagtctcg atcggcctgc ccctttgccc gctgggcggc gtccagctgc
     3181 tcctgcttcc cgctccgctc agtcttgagc cggtctgcct ccttctgctt ctcgacgctc
     3241 tccttgccga cgacgaagat cggcgggaca tcaccgcccc ccacggggaa cacgctctcg
     3301 tggatgaagt cccgattgaa cacccggatc ggaagcgtcg actgcgggaa gtcctcgc
//
LOCUS       DATMBB010000150         4019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_366126, whole genome shotgun sequence.
ACCESSION   DATMBB010000150 DATMBB010000000
VERSION     DATMBB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366126"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VNL92_03170"
     CDS             <1..408
                     /locus_tag="VNL92_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HWO93742.1"
                     /translation="VVAHPFHHHFDPVHFRRNGLKPFDLTPEQAAELPVFKLVDDVEV
                     SNSCNTPRENVFALKVARILGKPGFAGSDAHSRTGIGSSAVMFEKQLSSVDEFLAELH
                     AGRYRPTQGLHVDSFRLFEDIAREFEESAAAAS"
     gene            530..1285
                     /locus_tag="VNL92_03175"
     CDS             530..1285
                     /locus_tag="VNL92_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWO93743.1"
                     /translation="MAGHSKWAQIKRQKAVVDARRGQLFGKLARDIAVAARQGGSGDP
                     ALNFRLRLAVQKAREANMPNDNIERAIKRGVGEGGASELQEVAYEGYGPGGVAILVQA
                     LTDNRNRTVADVRTAFSRHGGNLGEAGCVAWQFSQKGQVTVPPGAGDPDEIGLLAIDL
                     GAEDFKVDTDGSMEIITAPENLESVRDGFAAAGIHIDDADLVMHPSQTIRIDARDADT
                     LLRLFDRLDELDDVQRVYSNADIPEEVLAAAGV"
     gene            1303..1812
                     /gene="ruvC"
                     /locus_tag="VNL92_03180"
     CDS             1303..1812
                     /gene="ruvC"
                     /locus_tag="VNL92_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWO93744.1"
                     /translation="MAAAGITVLGVDPGLGATGYGVVTMTGRAAAGIEHGCIRPSRRL
                     ALADRLLTIYEAMYELVRRHQPAAMAIEDPFIGENARSALALGRAQAVVMLAAASGGV
                     PVLTYSPATVKDAVTGYGRSDKQQVAEMVRMQLGMTHAPEPLDAADALAVALCHLAHA
                     ARPALRELT"
     gene            1809..2387
                     /gene="ruvA"
                     /locus_tag="VNL92_03185"
     CDS             1809..2387
                     /gene="ruvA"
                     /locus_tag="VNL92_03185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWO93745.1"
                     /translation="MITPIAQLEGTVVEAGSDGVVLMVGGVGYGLQVPAHALARLRPG
                     SVATFHTYLVAREDGMTLFGFLTPEERKAFAGLLTVSGIGPKAALTVLSAMSPRDLAA
                     AVAAGDLDALRRLPGIGPKTAARMVLELRGTLDGTGALASLQSADDDALLALLALGYS
                     AEEASRALDGAGGDVEERVRAALLRMGQGKSA"
     gene            2443..>4019
                     /gene="uvrB"
                     /locus_tag="VNL92_03190"
     CDS             2443..>4019
                     /gene="uvrB"
                     /locus_tag="VNL92_03190"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWO93746.1"
                     /translation="MNRFELVSDYQPTGDQPEAIERLVEGLERGYRHQTLLGVTGSGK
                     TFTMANVIARVQRPTLVLAPNRTLAAQLWAEFKEFFPQSAVEYFVSYYDYYQPEAYIP
                     RTDTYIAKDADINDEIDKLRHAATRALFERRDVLIVASVSCIYGLGEPEEYHSFVLTF
                     KKGESTNRNRAIRRLIDMQYERNDQNPVRGRFRLRGDSLTIHPAYEELAVRIDFWGDE
                     VERILQLDPLTGEIVSEREAIDIFPAKHFVTSQERLDAAMADVQAELEDRVAYLRSQG
                     KLLEAARLEERTRYDIESLREAGYCAGIENYSRHLQRRPQGSTPWTLLDYFPDDYLLF
                     IDESHMAVPQVRGMYHGDIARKQTLVDFGFRLPSALDNRPLNFAEFQQRTHQVIYVSA
                     TPGPYEYEHSEQIVEQIIRPTGLVDPTVEVRPTKGQIDDLIDEIKLRTDRGERTLVTT
                     LTKKMAEDLADYLREMGVRTHYLHSEIDTLERVATLRDLRMGVYDVVVGINLLREGLD
                     LPEVSLVAILDADKEGYL"
BASE COUNT          694 a         1333 c         1321 g          671 t
ORIGIN      
        1 gtcgtcgccc acccgttcca ccatcacttc gatccggtgc acttccgccg caacggcttg
       61 aagcccttcg acctcacgcc ggagcaggcg gcggaactgc ccgtcttcaa gctcgtcgac
      121 gacgtcgagg tgagcaacag ctgcaatacg ccaagggaga acgtcttcgc cctgaaggtc
      181 gcgcgtattc tcggcaaacc gggatttgcc ggcagcgacg cacactctcg gacgggcatc
      241 ggcagctcgg ctgtcatgtt cgagaagcag ctctcgagtg tggacgagtt cctcgccgag
      301 ctgcatgccg gccggtatcg tccgacgcaa ggcttgcacg tcgactcgtt ccgtctcttc
      361 gaggacatcg cgcgcgaatt cgaagagagc gctgctgcgg ccagctagca ccggttcgcg
      421 tccgttgccc cctgcgttca cgtccatggc gtaagtcgca cttgctttga ccggcggcaa
      481 gcctgctggt agaatcgacg tacatccgta ctactctggg gagcgtaaca tggccggtca
      541 ctcgaagtgg gcgcagatca agcgccagaa ggccgtggtc gatgcgcggc gcggccagtt
      601 gttcgggaag cttgcccgcg atatcgccgt ggctgctcgc cagggtggca gcggcgatcc
      661 cgcgctcaat ttccgcctcc gcctcgctgt gcagaaggcc cgcgaggcta acatgcccaa
      721 cgacaacatc gagcgggcca ttaagcgtgg cgtcggcgaa ggcggcgctt cggagctgca
      781 ggaggtcgcg tacgagggtt acggaccggg tggcgtggcc atcctcgtgc aggcgctcac
      841 cgataatcgc aaccgcaccg tagccgacgt gcgcacggcg ttctcgcgcc acggcggcaa
      901 cctcggcgag gcgggctgcg tggcctggca gttctcgcaa aagggccagg tgaccgttcc
      961 tcccggcgcg ggagacccgg acgagatcgg cctgctcgcc atcgacctcg gggcagagga
     1021 cttcaaggtc gataccgatg gctcgatgga gatcatcacc gcgccggaga acctcgagtc
     1081 ggtacgcgat ggcttcgctg ccgccggtat ccacatcgat gatgccgact tggtcatgca
     1141 cccttcgcag acgatccgga tcgatgctcg cgatgccgac accttgctcc gcctcttcga
     1201 ccggctcgac gagctggacg acgtgcagcg tgtctactcc aacgccgaca tcccggaaga
     1261 ggtgctggcg gccgcgggcg tatagggcca tcggaggcac atgtggcggc agccggcatc
     1321 accgtactgg gtgttgatcc cggcctcggc gccactggct acggcgtcgt cacgatgacc
     1381 ggccgcgccg ccgccggcat agagcatggc tgtatccgac cgtctcgtcg cctcgcgctg
     1441 gcagaccgct tgctgaccat ctacgaagcg atgtacgagc tggttcgccg acaccagcct
     1501 gctgccatgg ctatcgagga tccgttcatc ggcgagaacg ctcggtccgc tcttgctctc
     1561 ggacgtgctc aggccgtcgt catgcttgcg gcggcctccg ggggcgtccc ggtgctcacg
     1621 tactcgcccg cgacagtgaa ggatgcggtg accggctacg ggcgtagcga caagcagcag
     1681 gtcgcggaga tggtccgcat gcagctgggc atgacgcacg cgcccgagcc cctcgacgcc
     1741 gccgatgcgc tcgcggtggc cctctgccac ctggcgcacg cggcgcgtcc agccctgcgg
     1801 gagctgacgt gatcacgccg atagcgcaat tggagggcac ggtcgtcgag gcgggctcgg
     1861 atggcgtcgt gctgatggtc ggtggcgttg gctacggact acaagtgccg gcccacgcgc
     1921 tcgcgcggct gcgccccggc tccgttgcga cgttccacac ctacctggta gcccgcgaag
     1981 atgggatgac gctgttcggg ttcttgacgc cggaggaacg gaaggccttt gccggactcc
     2041 tcacggtcag cggcatcggc ccgaaggccg ccctcacggt gctctcggcc atgagcccgc
     2101 gagacctcgc ggcggccgtc gcggcaggcg atctcgatgc cctacgccgc ctgcccggca
     2161 tcggccccaa gacggccgcg cgcatggtgc tcgagcttcg cggcacgctc gatggcacgg
     2221 gcgccttggc atcgctgcag agcgccgacg acgacgcgtt gctcgcgctc ctggcgctcg
     2281 gttattcggc ggaggaggcg agccgggcgc tggacggcgc cggcggcgat gtggaggagc
     2341 gcgttcgcgc ggcgctgctg cgcatgggac aaggaaagtc cgcgtagtcc gcgacgagat
     2401 gtcctcgccc cgtcgcccca acgctatact cagagagaaa ctatgaaccg atttgagctc
     2461 gtctcggact atcagccgac cggtgaccag ccggaagcta tcgagcgcct cgtcgagggc
     2521 ctggaacgcg ggtaccgcca ccagacgttg ctcggtgtta cgggcagcgg caagacgttc
     2581 acgatggcga acgtgattgc gcgcgtgcaa cggcccacgc tggtcctggc gccgaatcgc
     2641 acgctcgccg cacagctgtg ggccgagttc aaggagttct tcccgcagag cgcagtcgag
     2701 tacttcgtct cgtactacga ctactaccag ccggaagcgt acatcccgcg caccgacacg
     2761 tacatcgcca aggatgccga tatcaacgac gagatcgata agctgcggca tgccgcgaca
     2821 cgcgccctgt tcgagcggcg cgatgtgctc atcgtcgcgt cggtctcctg catttacggt
     2881 ctcggcgagc cagaggagta ccattccttc gtcctcacgt tcaagaaggg cgagtccacc
     2941 aatcggaatc gtgccatccg ccggctcatc gacatgcagt acgagcggaa cgaccagaac
     3001 cccgtgcgcg ggcgctttcg cctgcgaggc gactcgttga ccatccatcc ggcctacgag
     3061 gagctggccg tccggatcga cttctggggc gatgaggtcg agcgtatcct tcagctcgat
     3121 ccgctcacgg gcgagatcgt gtcggagcgc gaggccatcg atatctttcc ggcaaagcac
     3181 ttcgtgacgt cccaggagcg gctcgatgcc gcgatggcgg acgtgcaggc ggagctggag
     3241 gaccgggtcg cgtaccttcg gtcgcagggg aagctgctgg aagcggcgag gcttgaggaa
     3301 cgcacgcgct acgacatcga gagtctccgc gaggccggat actgcgccgg gatcgagaac
     3361 tactcccgcc atctgcagcg gcggccgcag ggcagtacgc cctggacgtt gctcgactac
     3421 ttccccgatg actacctcct gttcatcgac gaatcgcaca tggccgtgcc acaggtgcgg
     3481 ggcatgtacc acggcgacat cgcccgtaag caaacgctcg tggatttcgg cttccgcttg
     3541 ccctcggcgc tcgataaccg gcccctgaac ttcgccgagt ttcagcagcg cacgcaccag
     3601 gtgatctacg tgtcggcgac gccgggccca tacgagtacg agcacagcga acagatcgtg
     3661 gaacagatca tccggccgac gggcctcgtc gacccgaccg tcgaggtgcg gccgaccaag
     3721 ggacagatcg acgacctcat cgacgagatc aagctgcgca ccgaccgcgg cgagcgaacg
     3781 ctcgttacga cgctgacgaa gaagatggcg gaggacctgg ccgactacct gcgggagatg
     3841 ggcgtccgca cgcactacct ccactccgag atcgacacgc tggagcgggt ggccacgctg
     3901 cgcgacctcc gcatgggcgt gtacgacgtc gtggtcggca tcaacctcct tcgcgaggga
     3961 ctcgacctgc cggaggtcag cctcgtcgcc atcctcgatg cggacaagga aggatacct
//
LOCUS       DATMBB010000151         6619 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_242414, whole genome shotgun sequence.
ACCESSION   DATMBB010000151 DATMBB010000000
VERSION     DATMBB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242414"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VNL92_03195"
     CDS             <1..379
                     /locus_tag="VNL92_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWO93747.1"
                     /translation="ERCLDEATNYSKIRVTFGQPLAQRQAIQWMLADSAIDIYATRLM
                     TYNTIWKFEQGEDIRSEISMVKVFATEMATRVADRAIQIHGGLGLTTDLPLERIYRGL
                     RIMRIVEGASEVHRMVVARRLLA"
     gene            394..1188
                     /locus_tag="VNL92_03200"
     CDS             394..1188
                     /locus_tag="VNL92_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO93748.1"
                     /translation="MALIYEKRNHIAYLTLNRPESMNALDPQTNEELADAWADFRDDP
                     EAWVAIITGAGDRAFCAGADLKATALHTPQAQAAPPSFAQMFFSTPRPSLMRGLELAK
                     PVICAVNGYALGGGMEMMLACDIRIAAEHATFGLTEARIGSIPGGGGTQRLPRQIPYA
                     LAMYMLLTAERITAEEALRFGLINKVVPLDKLMEEAEAVAEKIAANAPLSVRGIKTAV
                     QRGLDVPLQHGLLIEQQIFGMLFNSEDRIEGRRAFAEKRPPHYKGR"
     gene            1196..2338
                     /locus_tag="VNL92_03205"
     CDS             1196..2338
                     /locus_tag="VNL92_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWO93749.1"
                     /translation="MKDTCAIVGVGQTKQGTLPGTTPLGLGIEAFKNALDDAGLEKDD
                     IDGLLTMPGTTSPEGPLHYLRFGEAAGINPRFTASMVMGGGTAGVLVEYAVQAINAGL
                     ATTVACIFGDTAKTGGVRFGGASGSRGESWGLWGMFGPHANSAMGARRHMALYGTKSE
                     HLGMVAVAVRKHANLNPEAVMFDRPMTLQDHQESRWIVEPLHLLDCCLISDGGVCIIV
                     TSAERARSLKKPPVIISGMGQAYTTQNLMREDWWYVPHQADALRRAFEMAQVTPKDID
                     VAQLYDNFTISVLFWLEHAGFCKRGEAGPFVEGGRIELGGELPVNTAGGNLSESYMEG
                     WLHIVEGTRQVRGECGPRQVPNVEYCLTTGRGMALNCSNALILRKA"
     gene            2347..2766
                     /locus_tag="VNL92_03210"
     CDS             2347..2766
                     /locus_tag="VNL92_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWO93750.1"
                     /translation="MTTQQEYQKPLPIIEDDNRPFWEACKRHELVMQQCDACQTVWWP
                     IGPVCQRCLSDRWHWQKLSGRGELYSFVIFHQLFHPGYKDDIPYNVVMVRLEEGPHMF
                     SNLVGVPNDQIAVGAPVEVTFDDVTDEISLPKFKPRT"
     gene            2876..4705
                     /gene="lepA"
                     /locus_tag="VNL92_03215"
     CDS             2876..4705
                     /gene="lepA"
                     /locus_tag="VNL92_03215"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWO93751.1"
                     /translation="MDQSHIRNFCIIAHIDHGKSTLADRLLENTGTLTQREMAEQVLD
                     QMDLEREKGITIKAQAVRMVYHAEAGQDYELNLIDTPGHVDFAYEVSRSIAACEGAVL
                     VVDAAQGIQAQTLANVYLAMEQDLVIIPVVNKIDLPNAQPERVAQELCDVVGFDRDEV
                     LLVSAKDGTGVDRLLEAIVQRVPPPAGHVDSPLRALIFDSKYDSYKGVLAYVRVVDGA
                     VTTGRPLKLMSNDARFEPLELGTFRPAPLRVGSLTTGEVGYIATGLKDVRQCRVGDTV
                     TLVSNAAATPLPGYEPAKPMVFAGLYPSDSNEYPLLRDALNKLQLNDAALTYQPESSG
                     ALGFGFRCGFLGLLHMEIVQERLEREYDLDLLISAPTVEYQVTRTNGVEVTVHNPDEL
                     PAPNEISEVREPWMHITVIAPDRYIGAVMELVTGRRGEFRRMEYLEHGGGRDGVEGSR
                     RVLLEYDVPLAEILVDFYDDLKSRTQGYASLDYTFAAYRPAKVVKLEILVNGVPVDAL
                     SLITNAENASTLGRAIIERLKDAIPRQLFDVPIQAAIGGRIIARETIRALRKNVVAKC
                     YGGDVTRKRKLLEKQAAGKRRMKRVGRVEIPQEAFMSVLRLER"
     gene            4742..5284
                     /locus_tag="VNL92_03220"
     CDS             4742..5284
                     /locus_tag="VNL92_03220"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWO93752.1"
                     /translation="MRIVSSEPIHEQWPPISRHLVEFPNGQRRHWVSFDFPDGTAVLA
                     VTPDRRLVLTRQHLMGVDHTALMLPGGVAQEGESLEGCARRELYEETGYTAEEFVYVF
                     RYVNLPSYSRGWVHLYLARNARRDPSYRRDGAIEVDRVELVGVDEARERALGGELEAS
                     STMMAVLAVPHLLERLGWDS"
     gene            5301..6593
                     /locus_tag="VNL92_03225"
     CDS             5301..6593
                     /locus_tag="VNL92_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO93753.1"
                     /translation="METTPATTSAKPEPPIFTRDFALVCLATLLFFTAFLFYFPTLPT
                     FAERLGSAESEIGLLIAASSMTALVVRPFVGQLIDALGSRRLVVAGALLYVFTTLAYN
                     LADSMPLLIVVRIFNGMSLATFVTSTGAYVADIAPVARRGEAMGYYGLANNLAFAIGP
                     LIAVGVMDASALADAEEAINARAGWLAGAAAKPENFTLVFLLASGFALAAFVMSLRLR
                     ETGAARYPFRFVVNPADVFTRSALFPSAINFVSSFAFATIVTFVALFAKDNDMGGGAS
                     LFFLIYAASIMAMRLIGGRLSDRYGRGSMIVPGIAILAASLLVTGYAQHETMFWFGAM
                     LYGVGAGAAQPALMALTVDLAAPEERGRAMGTFTLSNDLGLSLGALALGVILEASNYE
                     TMYTVAAVIVLAGALIYVLGSGRLSKTATVDASSGASP"
BASE COUNT         1173 a         2197 c         2129 g         1120 t
ORIGIN      
        1 cgagcggtgc ctggatgaag ccacgaacta ttcgaaaatc cgcgtcacat tcgggcagcc
       61 gctggcccag cgccaagcga tccagtggat gctcgccgac tcggccatcg acatctacgc
      121 gacgaggctc atgacctaca acacaatctg gaagttcgag cagggcgaag acatccgctc
      181 cgagatatcg atggtcaagg tgttcgcgac cgagatggcc acacgcgtcg cggaccgcgc
      241 catccagatc cacggcggcc tcggcctaac gacggatctg ccgctcgagc ggatctaccg
      301 gggcttgcgc atcatgcgca tcgtcgaggg cgccagcgag gtccatcgca tggtcgtcgc
      361 gcggcgcctg ctggcgtagg gaaggagagc atcatggccc tcatctacga aaagcggaac
      421 catatcgctt atctgacgct caaccgcccc gaatcgatga acgccctcga cccccagacc
      481 aacgaggagc tcgccgatgc ctgggcggac tttcgggacg atccggaggc gtgggtcgcg
      541 atcatcacgg gggcaggcga tcgagccttc tgcgctgggg ccgacttgaa ggcgaccgcg
      601 ttgcacaccc cgcaggccca ggccgcacct ccttcgttcg cccaaatgtt cttcagcacc
      661 ccgcggccct cgctgatgcg cgggctcgag ctcgccaaac cggtgatctg cgcggtcaac
      721 ggctacgcgc tcgggggagg gatggagatg atgctcgcct gcgacatccg catcgctgcc
      781 gagcacgcga cgttcggcct caccgaggcg cgcatcggca gcatcccggg cgggggcggg
      841 acgcagcgcc tgccccgaca gataccctat gccctcgcga tgtacatgct gctcaccgcg
      901 gagcgcatca cggcggagga ggcgcttcgg ttcggcctca tcaacaaggt cgtcccgctc
      961 gacaagctga tggaggaggc ggaggccgtg gccgaaaaaa tcgcggccaa cgcacccctc
     1021 tcggtgcgcg gcattaagac tgccgtgcag cgcgggctcg acgtgcccct ccagcacggc
     1081 ctgctcatcg agcaacagat cttcggcatg ctcttcaaca gcgaggaccg catcgaaggt
     1141 cggcgtgcct tcgcggagaa gcggccgccg cactacaagg gacggtgaca cggcaatgaa
     1201 ggatacgtgc gcaatcgtcg gcgtaggaca gacgaagcaa ggtaccctgc ccggcacgac
     1261 gcctctcgga ttaggcatcg aggcgttcaa gaacgccctc gacgatgccg gcctcgagaa
     1321 ggacgacatc gatggcctgc tgaccatgcc cggcaccaca tcgccggagg gaccgctgca
     1381 ctatcttcgc tttggcgagg ccgcgggaat caatcctcgc ttcacggcga gcatggtgat
     1441 gggcggcggt acggccggcg tcctcgtcga atacgcggtg caggcaatca acgcgggctt
     1501 ggccacgacc gtcgcctgca tcttcggcga cactgcgaag acgggcggcg tccgattcgg
     1561 cggcgcttcg ggttctcgag gggagagctg gggcctttgg gggatgttcg ggcctcacgc
     1621 caacagcgcg atgggtgcgc gccggcacat ggccctctat ggcacgaagt cggagcacct
     1681 cggcatggtg gccgtggccg tgcgcaagca cgccaatctg aaccccgagg cggtcatgtt
     1741 cgatcggccg atgacgttgc aggatcacca ggaatcgcgc tggatcgtcg agcccctgca
     1801 cctcctcgat tgctgcctca tctccgatgg tggcgtctgc atcatcgtga catcggccga
     1861 gcgggcgcgc tcgctcaaga agccgcccgt catcatcagt gggatgggac aggcttacac
     1921 gacgcaaaac ctgatgcgcg aggactggtg gtatgtgccg caccaggcag acgccctgcg
     1981 gcgagccttc gagatggccc aggtgacgcc gaaggacatc gacgtcgcgc agctctacga
     2041 caacttcacg atctcggtgc tgttctggct ggagcacgcg ggcttctgca agcgcggcga
     2101 ggcgggaccg ttcgtcgaag gaggacgcat cgagctgggc ggcgagctgc cagtgaacac
     2161 ggccggtggc aacctgtccg agagctacat ggaaggctgg ctccacatcg tcgaagggac
     2221 acgccaggtc cgcggcgagt gcggtccgcg ccaagtgccc aacgtcgagt actgcctcac
     2281 gacgggacgg ggcatggccc tcaactgctc caacgcgctc attctcagaa aggcctaggt
     2341 ggatcgatga cgacgcaaca ggagtaccag aagcccctcc cgatcatcga ggacgacaac
     2401 cggcccttct gggaggcgtg caagcgccat gagctcgtga tgcagcagtg cgatgcctgc
     2461 cagaccgtct ggtggcccat cgggcccgtg tgccagcgat gcctcagcga caggtggcat
     2521 tggcagaagc tcagtggccg cggcgaactc tactccttcg tcatcttcca tcagctgttc
     2581 cacccggggt acaaggacga catcccctac aacgtggtca tggtccgcct cgaggaaggg
     2641 ccgcacatgt tctcgaatct cgtgggcgtg ccgaatgacc aaatcgcggt aggggcgccc
     2701 gtcgaggtca ccttcgacga cgtgacggac gaaatctcgc taccgaagtt caagccgcgg
     2761 acctgactcg tccggctcgc cgacagcccc actttccccg ctccctggct gccatcgtgg
     2821 ccgacgtggt cgcgaccatg cggcgtgcgg gcgccgtggt acgattgact ccggaatgga
     2881 tcaatcgcac atccgcaact tctgcatcat cgcccacatc gatcacggca agtcgacact
     2941 cgcagaccgc ctcctcgaga acacgggcac gctcacgcaa cgcgagatgg ccgagcaggt
     3001 gctcgaccag atggacctgg agcgcgagaa ggggatcacg atcaaggccc aggccgtccg
     3061 gatggtctac cacgccgagg cgggccaaga ttacgaactg aacctcatcg atacccccgg
     3121 ccacgtcgac ttcgcctacg aggtgtcgcg ttcgatcgcg gcctgcgagg gcgccgtgct
     3181 ggtcgtcgat gcggcgcagg gcatccaggc acagacgctc gccaacgtct acttggcaat
     3241 ggagcaggac ctcgtcatca tccccgtcgt gaacaagatc gatctcccga acgcccagcc
     3301 ggaacgcgtc gcacaagagc tgtgtgacgt cgtcggattc gaccgcgacg aggtcctgct
     3361 ggtctcggcg aaggacggaa ccggggtcga ccggctgctc gaggccatcg tgcaacgggt
     3421 gcctccgcca gcgggccacg tggacagccc gctccgagcc ctcatcttcg actccaagta
     3481 cgactcctac aagggcgtgc tcgcctatgt ccgcgtcgtc gatggtgccg tgacgacggg
     3541 caggccgctg aagctcatgt cgaacgacgc gaggttcgag ccgctcgagc tggggacgtt
     3601 ccgccccgct ccgcttcgag taggctccct gacgacgggc gaggtcggct acatcgcaac
     3661 cggactgaag gacgtgcggc agtgtcgcgt cggcgatact gttacgctcg tgtcgaacgc
     3721 ggcggccacg ccgctaccgg ggtacgagcc cgcgaagccc atggtcttcg ccggcctcta
     3781 cccgtccgat tccaacgagt atccgctgct gcgcgatgcg ctcaacaagc tgcaactcaa
     3841 cgacgcggca ctcacgtatc agcccgagtc cagcggcgcc ctcggctttg gcttccgatg
     3901 cggctttctc ggcctcctcc atatggagat cgtgcaggag cggctcgagc gtgagtacga
     3961 cctggatctc ctcatctccg cccccacggt cgagtaccag gtgacgcgga cgaacggggt
     4021 cgaggtcacc gtgcacaatc cggacgagct gcccgctccg aacgaaatct cggaggtacg
     4081 cgaaccgtgg atgcacatca cggttattgc acccgatcgc tacatcggcg cggtcatgga
     4141 gctcgtcacc ggccgccgtg gcgaatttcg tcgcatggag tacctggagc acggcggagg
     4201 acgcgacggc gtcgagggct cgcgccgcgt tctcctcgaa tacgacgtcc cgctggccga
     4261 gatcctcgtg gacttctacg acgacctgaa gtcgcggact caggggtatg cctcgctcga
     4321 ttacacgttc gccgcgtatc gcccggccaa ggtcgtcaag ctcgagatcc tcgtgaacgg
     4381 cgttccggtc gacgcccttt cgctcatcac aaatgccgaa aatgcatcca cgctcggtcg
     4441 cgccatcatc gaacgcctca aggatgccat tccccggcag ctgttcgatg tcccgatcca
     4501 agccgcaatc ggcgggcgca tcattgcacg cgagaccata cgcgccctca ggaagaacgt
     4561 cgtcgcaaag tgctatggtg gcgacgtcac caggaagcgc aagctgctgg agaagcaggc
     4621 cgcgggcaag cgccgcatga agcgcgtcgg ccgtgtcgag attccgcagg aagcgttcat
     4681 gtcggtgctg cggctcgaac gctgacgcaa cggacgagag tgcccgcgag cggaggggta
     4741 catgcgcatc gtctcgtccg agccgatcca cgagcaatgg ccgcccatct cgaggcacct
     4801 cgtcgagttc cccaacgggc agcgacggca ttgggtctcg ttcgactttc cggacgggac
     4861 ggccgtcctc gccgtgacgc ccgaccgaag gctcgtgctc acgcgccagc acctgatggg
     4921 cgtcgatcac acggccctga tgcttcccgg cggggtcgca caggagggcg agtcgctgga
     4981 gggatgcgca cgccgcgaac tgtacgagga gaccggctac acggcggagg agttcgtcta
     5041 cgtctttcgt tacgtcaacc tgccttcgta ttcccgaggg tgggttcacc tctacctagc
     5101 tcgaaacgcg cgccgggatc catcgtatcg acgcgacggc gccatcgagg tcgatcgcgt
     5161 tgagctcgtc ggcgtggacg aggcgcgtga gagggccctg ggaggagagc tcgaggccag
     5221 ctcgacgatg atggccgtgc tggccgtccc gcacctcctc gaacgactcg gatgggatag
     5281 ctagcgaagg ccggacgccc atggagacca ctcccgccac gacgagtgcg aaaccggagc
     5341 ctccgatctt cacgcgcgac ttcgcgcttg tctgcctggc cacgctgctc ttcttcacgg
     5401 cgttcctctt ctacttcccc acgctgccga cgttcgcgga gcggctgggg agcgccgagt
     5461 ccgaaatcgg cctgctcatc gcggcgtcga gcatgacggc gctcgtggtt cggccgttcg
     5521 tcggacagct gatcgacgcg ctcggcagtc gcaggctggt cgtggcaggc gccctgctgt
     5581 acgtgtttac caccctcgcg tacaacctcg ccgattcgat gccgctgctc atcgtcgtgc
     5641 gcatcttcaa cgggatgtcg ctggcgacgt tcgtcacatc caccggggcc tacgtcgccg
     5701 acatagcgcc cgtggcgagg cgaggagagg ccatggggta ctacggcctg gcgaacaatc
     5761 tcgccttcgc cattggcccg ctcatcgctg tcggcgtcat ggacgcgtcc gccttggccg
     5821 atgccgagga ggcgatcaac gcgcgagcag gctggctcgc cggcgcggca gcgaagccgg
     5881 agaacttcac gctcgtgttc ctgctcgcga gcgggttcgc cctcgccgcg ttcgtcatgt
     5941 cgctccgcct gcgagagacg ggcgcggcac gatacccgtt ccgcttcgtc gtcaacccgg
     6001 cggacgtctt cacccgctcc gccctgttcc cctcggccat caatttcgtt tcgtcattcg
     6061 ccttcgcgac aatcgtgacg ttcgtcgcgc tgtttgcgaa agacaacgac atgggcggcg
     6121 gcgcaagcct cttcttcctc atctacgccg cttcgatcat ggcaatgcgc ctcattggtg
     6181 gacggctgtc tgatcgttac ggccggggct cgatgattgt gcccggcata gcgatactcg
     6241 ccgcatcgct tctcgtgacg ggctacgcgc agcatgaaac gatgttctgg ttcggcgcga
     6301 tgctgtacgg ggtcggagcg ggggcggccc agccggcgct catggccctg accgtcgacc
     6361 tggcagcgcc cgaggaacgt gggcgggcca tgggtacgtt cacgctgagc aacgaccttg
     6421 gcctgagcct cggggcgctc gcgctcggcg tcattctgga ggccagcaac tacgagacga
     6481 tgtacacggt ggcggccgtc atcgtgctcg cgggcgccct catctacgtg ctcgggagcg
     6541 ggcgcctctc gaaaaccgcg actgtcgacg cgtcgagtgg cgcctcgccc tgattcgggt
     6601 gcgcttgccg cggcccgca
//
LOCUS       DATMBB010000152         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_309740, whole genome shotgun sequence.
ACCESSION   DATMBB010000152 DATMBB010000000
VERSION     DATMBB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309740"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VNL92_03230"
     CDS             complement(<1..587)
                     /locus_tag="VNL92_03230"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWO93754.1"
                     /translation="MTDNEPREGDLVRAAGIIRRARYSIALVGAGMSRESGIPTFRGD
                     DGLWTRYGEPDMMGYQRFVEDPKGWWERRNAPDPRMQELAVALESARPNPGHYAMAEM
                     ERLGYLKHIITQNVDNLHHEAGSLSVTEIHGNRHKLRCLSCGQRFWLTEFTFTELPPR
                     CPHCGGLIKSDTVMFGEPIPSDALQTCFAESEKAD"
     gene            complement(584..982)
                     /locus_tag="VNL92_03235"
     CDS             complement(584..982)
                     /locus_tag="VNL92_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWO93755.1"
                     /translation="MERVLVRTDARESLRDITADVCSAIARTGVQDGLAVLFVPHTTA
                     AITVNENADPDVLADLTGHLRTLAPEDGGFRHAEGNADAHIKASIVGPSESLIIERGR
                     PQLGRWQGIFLCEFDGPRTREVWVQVAATR"
     gene            complement(1027..1233)
                     /locus_tag="VNL92_03240"
     CDS             complement(1027..1233)
                     /locus_tag="VNL92_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93756.1"
                     /translation="MAKDPVCGAEVDEARVNQQAGTVQAGAPQTDPVYGTKRFHEGTW
                     YYFCSLACRQKFMASPGQYVTPSR"
     gene            complement(1249..2358)
                     /locus_tag="VNL92_03245"
     CDS             complement(1249..2358)
                     /locus_tag="VNL92_03245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93757.1"
                     /translation="MDLELTETQQILQRTARDFLAKELSFRHVRELETSPTGHTPELW
                     ARMADLGWLGLPFPSEYGGADGDLLDLAALVEEMGRAAAPTPYLPTIVSGLAVLHHGT
                     EEQKRELLPRIARGELLLSPAIVEPGGGYRPESIEFVARPDGGRYVLNGTKLFVEFGH
                     IADFVLTAARSDGALALFLVPPSTGDIMRRPMPTTGLERHTEIVFADVKAGLETLLGG
                     PGDRSAALRDLIDLATAMTSVQLAGIAQRTLEMSVEYVSMRVQFGRPIGSFQAVQHHI
                     ANMATLAAAARLAAYEAVWKLQQGIAKPADIAYAKAIANNAREVTMTAHQLHGGIGYM
                     KEYDLQFFSRAAASGALRYGTTDDALAEVERALGL"
     gene            complement(2369..>2757)
                     /locus_tag="VNL92_03250"
     CDS             complement(2369..>2757)
                     /locus_tag="VNL92_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93758.1"
                     /translation="AFAKEAKIGGRRPADDPIVRRRLAELAVDLQITRLLALQNAYII
                     DKGMVPTKEASMVKVFNSELRYKVACVGMDIMGPFGQVQRGSEWAPLQGRIESLFRAA
                     PVARFGAGTNEVQRNIIAQRGLGLPR"
BASE COUNT          433 a          905 c          923 g          496 t
ORIGIN      
        1 caatccgcct tctccgactc ggcgaaacac gtttggagcg cgtcgctcgg gatcggctcg
       61 ccgaacatga cagtgtcgct cttgatcagg cccccgcagt gggggcagcg aggcggcaac
      121 tcggtgaacg tgaattcggt cagccagaac cgctggccgc aactcagaca gcgcagcttg
      181 tgccggttcc cgtggatctc ggtgacggag aggctccccg cctcgtgatg gaggttgtcg
      241 acgttctggg taatgatgtg cttcaggtag cccaagcgct ccatctccgc catggcgtaa
      301 tgccccggat tcgggcgggc cgattccagt gccacggcca gctcctgcat acgggggtcc
      361 ggcgcgttgc ggcgctccca ccatcccttc gggtcctcca cgaagcgctg gtaccccatc
      421 atgtcgggct caccgtagcg agtccagagc ccgtcgtcgc cgcggaaggt ggggatgccg
      481 ctctcccgcg acatgccggc gcccacaagc gcaatcgagt agcgcgcgcg gcggatgatg
      541 cccgccgccc ggacgaggtc gccttcccgc ggctcgttgt cggtcatcgt gtcgccgcca
      601 cctgtaccca tacttctcgt gtgcgggggc catcgaactc gcagagaaag atgccttgcc
      661 agcgcccgag ctgcggacgc ccacgctcga tgatgaggga ctcggacggc ccaacgatcg
      721 acgctttgat gtgcgcgtcg gcgttcccct cggcgtggcg gaagccgccg tcctcggggg
      781 caagggttcg gaggtgacca gtcaggtcgg cgagcacgtc ggggtcggca ttttcgttga
      841 ccgtgatggc cgccgtcgtg tgcggaacga agagcacggc gagcccgtcc tgcactccag
      901 tgcgtgcgat ggccgagcaa acatccgccg tgatgtcgcg gagcgattcg cgggcgtcgg
      961 tccgaacgag aacgcgttcc atgtcatcca tgataccgtg gccggagcct gtgcaggtgc
     1021 gcagggtcag cgcgacggcg tgacgtattg gcccggcgac gccatgaact tctgacggca
     1081 ggccagcgag cagaagtagt accacgtacc ttcgtggaag cgcttcgttc cgtacaccgg
     1141 gtcggtctgc ggtgcgccgg cctgtaccgt gcctgcctgc tggttcacgc gcgcctcatc
     1201 gacctcggcg ccgcagaccg gatccttcgc catgtctcac ctcactcgtc agagacccag
     1261 tgcgcgttct acctcagcga gcgcatcgtc cgtcgtgccg taacgaagcg cgcccgaagc
     1321 ggccgcccgg ctgaagaact gcagatcgta ctccttcatg tacccgatgc cgccgtgcaa
     1381 ctggtgggcg gtcatcgtga cctcgcgggc gttgttcgcg atggccttgg cgtaggcgat
     1441 gtcggccggc ttggcgatgc cctgctggag cttccacacg gcctcgtacg ccgcgagtcg
     1501 tgcggccgcg gcgagtgtgg ccatattggc gatgtgatgc tggacggcct ggaacgagcc
     1561 gatagggcgg ccgaactgca cgcgcatcga cacgtattcc accgacatct cgagcgtacg
     1621 ctgggcgatg ccggcaagtt gtaccgatgt catcgccgtc gccagatcga taaggtcgcg
     1681 aagtgccgcg ctccggtcgc ccggtccgcc caggagagtc tcgaggccgg ccttgacgtc
     1741 ggcgaagact atctccgtat gtcgctcgag gccagttgtt ggcatggggc gccgcatgat
     1801 gtcgcccgtg ctgggcggaa cgaggaagag agcgagcgcg ccgtcggagc gcgccgccgt
     1861 gagcacgaaa tcggcgatgt ggccgaactc gacgaagagc ttcgttccat tgagcacata
     1921 gcgtcctcca tcggggcgcg ccacgaactc gatgctttcg ggccgatatc cgccgcccgg
     1981 ctcgacgatc gcaggcgaca ggaggagttc gcccctcgcg attcgcggca gaagctcgcg
     2041 cttctgctcc tcggtgccgt ggtgcagcac ggcgagtccc gacacgatcg taggtaggta
     2101 cggcgttggc gcggcggcac gccccatctc ttcgaccagg gccgcgaggt cgaggaggtc
     2161 gccatcggcc cctccgtatt cgctggggaa cggcaggccg agccacccga gatcggccat
     2221 cctcgcccag agttcgggcg tgtggcccgt cgggctcgtc tccagctcgc ggacgtggcg
     2281 gaaggagagc tccttcgcga ggaagtcgcg agccgttcgc tgcaggattt gttgcgtttc
     2341 ggtgagctcg aggtccatcg ttctgccctt atctgggaag gccgaggccg cgctgcgcga
     2401 tgatgttgcg ttgcacctcg ttggtgcccg caccaaagcg ggccacgggg gccgcacgga
     2461 acagcgactc gatgcggccc tgcagcggcg cccattccga tccgcgttgt acctgtccaa
     2521 atgggcccat gatatccatg cctacacacg cgaccttgta ccgcaactcg ctgttgaaca
     2581 ccttcaccat cgaggcttcc ttcgttggca ccatgccctt gtcgatgatg taggcgttct
     2641 gcagcgcgag gagccgcgtg atctggagat cgaccgcgag ctcggcaagc ctgcggcgca
     2701 cgatcgggtc atcggcgggc cgccggccgc cgatcttcgc ctccttggcg aaggcca
//
LOCUS       DATMBB010000153         3184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_449003, whole genome shotgun sequence.
ACCESSION   DATMBB010000153 DATMBB010000000
VERSION     DATMBB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449003"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VNL92_03255"
     CDS             complement(<1..697)
                     /locus_tag="VNL92_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HWO93759.1"
                     /translation="MGLKTFGLRAVDWEERVNYQRLRTARLDRIRHLLRASELGALLC
                     FDMNNIRYITATHIGTWAMDKLVRFALLPQDDDPIMWDFGSAARHHALYCPWLGDRSR
                     AGISTLRGAMAPGSGRAEDVARKIRIELEERGLVKEPLGVDVIELPVLFALQAEGIKV
                     VDGQQLMQDARIIKTEDEITLLNTACMMVDAAYEELYRAMKPGMRENECVAIANKVLY
                     DLGSEHVEGVNAIS"
     gene            complement(718..1698)
                     /locus_tag="VNL92_03260"
     CDS             complement(718..1698)
                     /locus_tag="VNL92_03260"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWO93760.1"
                     /translation="MRELTFGQAVKEALAEELRRDERVFIIGEDVAEAGTTFKVLLGL
                     VDEFGPERVMDSPISEAGITGLAVGAAMTGMRPVVDIMFGDFLTLAMDQIVNQAAKVH
                     YMSGGRLRVPMVVRTTMGAGRRSAAQHSQSLHAFVAHVPGLKVVVPATPYDAKGLLKS
                     AIRDDNPVIFFEDKMMYQLKGPVPSDDYTVPLGVAEVKRAGEDVTLVATSSMVHVGLA
                     AADRLESIGIRMEVVDVRTLVPLDSHTLVESAKKTGRAIVVDEGYQRYGVTAEIASVI
                     AQGAFYQLDAPVARIGAMDVPIPFSPPLEDLTIPTAERVVEVARALCGRA"
     gene            complement(1730..2653)
                     /locus_tag="VNL92_03265"
     CDS             complement(1730..2653)
                     /locus_tag="VNL92_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWO93761.1"
                     /translation="MLTIRSFEEHVNDLYTRAKMPGLAHLYIGQEAVAVGVCAALRRD
                     DYVTSTHRGHGHCLAKGAAVDRMFAELLGKAAGYCRGKGGSMHIADPETGNLGANAIV
                     GGSAGIATGAALSIKRRRSGQVAVCFFGEGAFGQGLLYEVMNMASLWTLPVVYVCENN
                     LYNEYTPVRESTAGDFRARVEAFGIVVDEVDGQDVRAVHAAAARLVERARSGSGPGFL
                     LCHTYRFRGHHVGDVNRTFYRSKDEERAWATRRDPLSILADWLVSQGIAGRDDLQQDE
                     REVRAEVTAGVEFALAAPYPSLDEVDSHVYA"
     gene            complement(2843..>3184)
                     /locus_tag="VNL92_03270"
     CDS             complement(2843..>3184)
                     /locus_tag="VNL92_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93762.1"
                     /translation="ASEVAQVRERLSALSAPAGRTVEVSTRVTTRVLDVAMTSATAPT
                     REEGYYRAADAWSGIVGSGPQLLEQVKEYAAAGSTHFIAQFEHETVDQHVRAIRAFAE
                     AVITPWRRGER"
BASE COUNT          513 a         1155 c          969 g          547 t
ORIGIN      
        1 ccgagatggc gttcacgccc tcgacgtgct ccgaccccag gtcgtagagc accttgttgg
       61 cgattgcgac gcactcgttt tcccgcatgc ccggcttcat cgctctgtac agctcttcat
      121 aggccgcgtc gaccatcatg caggccgtgt tgagcagcgt gatctcgtcc tccgttttga
      181 tgatccgcgc gtcctgcatc agttgctggc catcgacaac cttgattcct tcagcctgga
      241 gggcgaagag cacgggcagc tcgatcacgt ctacgcccag gggctccttc acgagcccgc
      301 gctcctcgag ctcgatccgg atcttccggg ccacgtcctc cgcccgcccc gacccaggcg
      361 ccatcgctcc tcgcagtgtg gagatgcccg cccgcgatct gtctcccaac cagggacagt
      421 agagcgcgtg gtgtcgcgcg gcggatccga agtcccacat gatcgggtcg tcgtcctgcg
      481 gcaacaaggc gaaccgcacg agcttgtcca tggcccatgt cccgatgtgg gtggccgtga
      541 tgtaccggat gttgttcatg tcgaagcaca gcagcgcgcc gagctccgac gccctcagca
      601 ggtgcctgat tcggtcgagg cgagccgtac gcaggcgctg gtagttcacg cgctcttccc
      661 agtcaaccgc tctcagtccg aacgtcttga gccccatgag cgtcctcctt ggtgggacta
      721 ggctctcccg cagagggctc tggccacttc gacgacgcgc tccgccgtcg gaatagtcag
      781 gtcctcgagg ggaggagaaa aaggaatcgg cacgtccatc gcgccgattc gggccaccgg
      841 agcgtcgagt tggtagaacg cgccttgggc gatcaccgat gcgatctcgg cggtgacgcc
      901 gtaccgctga taaccctcgt caacgacgat ggcccgcccg gtcttcttcg cggattccac
      961 cagcgtgtgc gagtcgaggg gaaccagcgt ccggacatcg acgacctcca tacgaattcc
     1021 gatcgactcc agacgatcgg ccgcggccag gcccacatga accatgctgc tcgtggcgac
     1081 aagggtcaca tcctcgcccg cccgcttcac ctcggccacc cccagcggga ccgtatagtc
     1141 gtccgaaggc acagggccct tgagctggta catcatcttg tcttcgaaga agatgaccgg
     1201 gttgtcgtcg cggatcgcgg acttcaggag tcctttcgcg tcatagggcg ttgcgggcac
     1261 caccactttg agccctggca catgggccac gaaggcgtgc aggctctggc tatgctgcgc
     1321 ggccgagcgg cggccggcac ccatcgtcgt gcggacgacc attggcacgc ggagcctccc
     1381 gccggacatg tagtgcacct tcgccgcctg gttgacgatc tgatccatcg ccagcgtgag
     1441 gaaatcgccg aacatgatgt cgacgacggg ccgcataccg gtcatcgctg cgcccaccgc
     1501 caggcccgtg atgccggctt cggagatggg cgaatccatg acgcgctcgg gcccgaactc
     1561 gtccaccaga ccgagcagca ccttgaaggt cgtcccggct tcggccacgt cctcgccgat
     1621 gatgaagacc cgttcgtccc gccgcagttc ctccgcgagg gcctccttga cggcctggcc
     1681 gaaggtgagc tctcgcaggc cggcgtgcgg cggcgcgacc ggcgccgttt cacgcgtaga
     1741 catggctgtc cacctcgtcc agcgacggat agggagcggc aagggcgaac tccacacccg
     1801 cggtgacctc cgcgcgcacc tcacgctcgt cctgctgcag gtcgtcacgg ccggcgatcc
     1861 cctggctcac gagccagtcg gccaggatcg agagcggatc ccgccgggtc gcccaggcgc
     1921 gctcctcgtc cttggagcgg tagaacgtcc gattgacgtc ccccacgtga tgcccacgga
     1981 agcggtacgt atgacacagg aggaaaccgg ggccgctccc cgacctcgcc cgctctacga
     2041 gcctcgcggc cgccgcgtgg acagcccgga catcctgccc atccacctca tccaccacga
     2101 tgccgaacgc ctcgacgcga gcccggaagt cgccggccgt ggattcccgc actggcgtat
     2161 actcgttgta caggttgttc tcgcagacat acacgaccgg cagcgtccag agcgacgcca
     2221 tgttcatgac ctcgtagagc agcccttgtc cgaacgcccc ttccccgaag aagcacaccg
     2281 ccacttgccc cgaccgccgc cttttgatcg acagcgccgc gcccgtcgcg atccccgcgc
     2341 tgccgccgac gatggcgttg gcccccaggt tgccggtttc aggatcggcg atgtgcatgg
     2401 acccgccctt gccccggcag tacccggccg ccttcccgag gagctccgca aacatgcggt
     2461 ctacggcggc ccccttcgcc aggcagtgtc cgtgaccgcg gtgcgtgctc gtcacatagt
     2521 catcgcggcg caaagccgcg cagacgccga ccgcgaccgc ctcctgcccg atgtagagat
     2581 gagccagacc gggcatcttc gctctggtgt agaggtcgtt gacatgctcc tcgaagctgc
     2641 ggatcgtgag catcgtcctg tagagcgcgc gccaggagac cggccgagcg cgaccgtcat
     2701 ccgccgaatc cctgatggat gctgatgcct gcgtcatctc tctcctccaa ccgttcgtgg
     2761 gcttcccggg cacgcgccac cgtgacaccg cggcgatccc gtaccggtcg cttcccacgt
     2821 cccgttcccc cctcggcgag gctcagcgct cgccgcgccg ccacggcgtg atcaccgcct
     2881 cggcaaacgc ccgaatggct ctgacgtgct gatcgaccgt ctcgtgctcg aactgcgcga
     2941 tgaagtgggt ggagccggcc gccgcgtact ccttcacctg ttccaggagc tgcggcccgc
     3001 tgccgacgat acccgaccat gcatccgccg cccggtagta tccctcctct cgcgtcgggg
     3061 cggtggccga ggtcatggcg acgtccagga cccgggtcgt cacccgagtc gatacctcca
     3121 ccgtacgccc cgcgggcgcg gacagcgcgg acaagcgctc gcgcacctgg gccacctcgg
     3181 aggc
//
LOCUS       DATMBB010000154         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_138331, whole genome shotgun sequence.
ACCESSION   DATMBB010000154 DATMBB010000000
VERSION     DATMBB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138331"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VNL92_03275"
     CDS             <1..815
                     /locus_tag="VNL92_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93763.1"
                     /translation="PNGAGKTSTIRMITCVSPPSRGELRVAGFDVARQPRAIKSRIGV
                     VPQDDNLDPDLTVEENLRSHARFFGIERRIREQRIAESLELFQLAERRSARVDELSGG
                     MKRRLVIARALLNEPEMVVLDEPTTGLDPQARHVVWQKLRILRGRGITLLLTTHYMDE
                     AAILCDRIAIMDQGRIVAEGSPSELIARHAGTEVVELRVPPGQAIGLAQHLSRMSDLD
                     VEATGDTVYVFDTNGLQAVAILEHLETAHAVSTRPANLEDVFLRLTGRALEE"
     gene            819..1616
                     /locus_tag="VNL92_03280"
     CDS             819..1616
                     /locus_tag="VNL92_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93764.1"
                     /translation="MAVLSPVARVRTIWPTWRFLAVWQRHLDAAQHTWVSAALMPAVE
                     PLLTFLVLGFGLGQFVELEGGQEYRDFIAPGMLAAFPMWQAVFVCTWEAWMRMESNRT
                     YHAMIATPVEPEEVAAGDVAWAATLGTLSTSYVFLMALAFGAINSPLALLILPLSLVH
                     TAMLGSMALTYTTAIGNSSNLNYFFTIVVLPMFWFGDVFVPIDTLPEGLQALGWFVPT
                     THTTAIYRGLMNGEVELTMVGDLAWLGAAFAVFVLLAMRFMRRRLIT"
BASE COUNT          400 a          746 c          669 g          408 t
ORIGIN      
        1 ggcccaacgg ggccggcaag accagcacca tccgcatgat cacgtgcgtc tcgccgccaa
       61 gccgcggcga gctccgtgtc gccggcttcg acgtggcgcg gcagccgcgc gcgatcaagt
      121 cgcgcatcgg cgtcgtcccc caggacgaca acctcgaccc cgacctgact gtggaggaaa
      181 acctccgctc gcacgcgcga ttcttcggca tcgagaggcg catccgcgag cagcggatcg
      241 ccgagagcct ggaactgttc cagctcgccg aacgccgctc cgcgcgggtc gacgagctct
      301 ccggcggcat gaagcgccgc ctcgtcattg cccgcgccct gctcaacgag cccgaaatgg
      361 tcgtgctcga cgaaccgacg acggggctcg acccgcaggc gaggcacgtc gtgtggcaaa
      421 agctccgcat tctgcgcggc cggggcatca cgcttctgct cacgacccac tacatggatg
      481 aggccgccat cctgtgcgat cgcatcgcca tcatggacca ggggagaatc gtggccgagg
      541 gttcgccgag cgagttgatc gcgaggcacg ccggcaccga ggtcgtcgag ctccgcgtcc
      601 cgcccgggca ggcgattggc ctcgcccaac acctgtccag gatgtccgac ctcgatgtgg
      661 aggccaccgg cgacaccgtc tatgtgttcg atacgaatgg cctacaggca gtggctattc
      721 tggagcacct cgagacagcc cacgccgtga gtacgcggcc ggccaacctc gaggatgtgt
      781 tcctgcgcct cacgggccga gccctggagg aatagcgcat ggctgtgctc tcgcctgttg
      841 cgcgggtccg gacgatttgg ccaacatggc gcttcctcgc cgtgtggcag cgtcacctcg
      901 atgcggctca gcacacttgg gtttccgccg cgctgatgcc ggcagtggag ccgctcctca
      961 cgttcctcgt gctcggattc gggctcgggc agttcgtcga gctcgaaggc ggccaggaat
     1021 atcgcgattt cattgccccg ggcatgctgg ccgccttccc catgtggcaa gccgtcttcg
     1081 tatgcacgtg ggaagcgtgg atgcgtatgg aaagcaaccg gacctaccac gccatgatcg
     1141 caacgccggt ggagcccgaa gaggtggcgg cgggggatgt cgcatgggcg gcgaccctgg
     1201 gcaccttgag tacgtcctac gtgttcctca tggcgctcgc gttcggcgcc atcaattccc
     1261 cgctggcctt actcatactg ccgctgtcgc tcgttcacac ggcgatgctc ggctccatgg
     1321 cgctcacgta tacgacggcc atcggcaaca gcagcaacct gaactacttc ttcaccatcg
     1381 tcgtcctgcc gatgttctgg ttcggcgatg ttttcgtgcc gatcgacacg cttccagaag
     1441 gattgcaagc actcggttgg ttcgtgccga caacccatac caccgccata tatcgcggcc
     1501 tgatgaacgg cgaggtcgaa ctcaccatgg ttggcgattt ggcctggctt ggcgctgcct
     1561 tcgccgtgtt cgtcctgctc gcaatgcgct tcatgcggcg gcgcttgatc acgtaatcgc
     1621 tttcgtccac acacagatgc ggcgcctcac gaacgtgccg cgcagcgccg caccatccct
     1681 ttgccgggtt ggtctacgcc cccatcggcg tggacgggac gatcggaccg agaagcccgc
     1741 caatgagcga caacaggacc tgtatcagac ctaggaacaa gtccagcggg cctccttggg
     1801 ccgatactgt cggcgacgac aacgccatca ccgataaggc gatgagcgcc gcccctacta
     1861 cccacgcgta ccgattcgac cacgatagcg tcacgttcgg tctcctccct cgaattggtg
     1921 atcatcgcga attcttagcg ccatagcgtg aaagaagcct tgctatcatt cccaatgcat
     1981 aacgtgtgta taacaatgct tttatgcgaa gctacgccca gggttgagaa atcgcgtcac
     2041 gccatcccgc ccttagaacg cgaatccctg gaccaataca gcagcggccg agtttgacag
     2101 gctgtccgac gccacctacg atttccctca tgaccgccac gcgggatgga cgcctccgcg
     2161 tcttggatgg ctccaatcgc ggctggcgga cgcgggcctc gggataaagg gaacagcagc
     2221 atg
//
LOCUS       DATMBB010000155         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_525580, whole genome shotgun sequence.
ACCESSION   DATMBB010000155 DATMBB010000000
VERSION     DATMBB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525580"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VNL92_03285"
     CDS             <1..590
                     /locus_tag="VNL92_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93765.1"
                     /translation="FLGTQIMDEGRHLDVFRKRALANGGGLMEGLGFAAVGSIGSSDF
                     TEASTFLHIIGEGFVQSMFRMGEYISQNEAEKRIFRRSAEDESRHVAFGVMHMKHLID
                     TQPERREEIHHYLDRGESQTLNSQAGVTFNASTMESLAVLLGGGKANFEEGTKLLMAI
                     RKRQIKEYVHRLEVCGLGERVTDGRLAPALAALLN"
     gene            718..1923
                     /locus_tag="VNL92_03290"
     CDS             718..1923
                     /locus_tag="VNL92_03290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWO93766.1"
                     /translation="MAGIRVMISRAGREPVDPRMSQGRVDTTVRCVPIALVALLLLAG
                     CASGATSGGEATAPPQRLPLRCQTDCEPFEIVFTGDVLLGDAAQPWLERYGFAWAFEH
                     MAPLLRADAVIGNLEGPLTTRTRRQYSDQRWSYQADPKAAQALAEVGFDAFGFANNHT
                     YDRGPIGLADTLRHAREAGLAIFGAGLDAAEAAAPLLIETPYGDVGVVAFTSGARFGA
                     EAGPSHPGMALLSPEAAERGYRLAREAGARWVIAFVHWGENYATVTDEQRRRARVLAS
                     AGYDLVVGHHPHIAQGAEWIDDTLVLYSLGNFVFGTPGRFGPSAPGYGLVARVVLDGD
                     GIDRVELTGIVTDNDIVAFRPRPCDPASSDAILESLDSPTAAFCGTALIEWPRGTTLP
                     SAATRATRP"
     gene            complement(1910..>2410)
                     /locus_tag="VNL92_03295"
     CDS             complement(1910..>2410)
                     /locus_tag="VNL92_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93767.1"
                     /translation="TVMIEGDSATLTLEGDGITFGVGLIKDGDEWKADRLFVPNDDIP
                     SGTEVVEIGLAEFAFNGDLESPAVTSGDFAFHVTNEGQQPHELVLVALPEGRPIEELV
                     QDESFQPEPILVRTTYEPGDESDVALPAPLEPGRYGLVCFLPDTDDPEGTPHAFKGMV
                     AEFTVE"
BASE COUNT          399 a          840 c          774 g          397 t
ORIGIN      
        1 tgttcctcgg cacccagatc atggacgagg gacgccacct cgacgtgttc cgcaagcggg
       61 cactcgccaa cggcggcggc ctgatggaag ggctaggctt cgcagcagtc ggcagcatcg
      121 gctcaagcga ctttacggaa gcctcgacgt tcctccacat catcggtgag ggcttcgtcc
      181 agtcgatgtt ccggatgggc gagtacattt cccagaacga ggcggagaag cgcatcttcc
      241 gccggtccgc cgaagatgag agccggcacg tcgccttcgg cgtcatgcac atgaagcacc
      301 tcatcgacac acagccggaa cggcgcgagg agattcacca ctacctcgac cgcggcgaga
      361 gccagacgct caactcccaa gcaggcgtga ccttcaacgc gtccaccatg gagtcgttgg
      421 ctgtgctcct cggtggcggc aaggccaact tcgaggaggg cacgaagctc ctgatggcga
      481 ttcgcaagcg ccagatcaag gagtacgtgc accggctcga ggtatgcgga ctcggcgagc
      541 gcgtgacgga tggccgtctg gccccggcgc tcgccgcgct cctcaactaa gccggcgcat
      601 cgttcggaac cggaaagcgg cctcccacag ggaggccgct ctcgcatctt cggctcgctc
      661 gaccgcgcgc gccgccaccg ctgataatga ggccatcgct ccgctcctga ggcgaggatg
      721 gcaggcatcc gtgtcatgat aagtcgcgcc gggcgagagc ccgtcgaccc tcgcatgagc
      781 caaggccgcg tcgacacgac cgtccgatgc gtaccgatcg cgctcgtcgc gttgctcctc
      841 ctcgcgggct gcgcttctgg cgctacaagc ggaggcgagg cgaccgcgcc gccgcagcgt
      901 ctgccgctcc gctgccaaac cgactgcgag ccgttcgaga tcgtcttcac cggcgatgtg
      961 ctgctcggcg acgcggcgca gccgtggctc gagaggtatg gattcgcctg ggccttcgaa
     1021 cacatggcgc cgctgctgcg cgccgacgcg gtcatcggta atctagaggg tccgctcacg
     1081 acgcgaactc ggcggcagta cagcgatcag cgatggagct accaggcgga tcccaaggcc
     1141 gcgcaggccc tcgccgaggt gggattcgat gctttcggct tcgcgaacaa ccacacgtac
     1201 gaccgcggcc ccatcggcct cgcggatacg cttcgccacg ctcgcgaggc cggactcgcc
     1261 atcttcggtg ccggcctcga cgcggccgag gcggccgcac cgctgctcat cgaaaccccg
     1321 tacggcgatg tcggtgtggt tgccttcacc agcggcgcgc gcttcggtgc cgaggccggc
     1381 ccatcccatc ccggcatggc gcttctcagc cccgaggccg ctgagcgcgg ctatcgtctc
     1441 gcacgggagg cgggggcgcg gtgggtcatc gcctttgtgc actggggcga gaattacgcc
     1501 actgtcacgg acgagcaacg ccgacgggcg cgtgtgctgg cctcggccgg ctacgacctc
     1561 gtcgtggggc accacccgca catcgcccag ggcgccgagt ggatcgacga cacgctcgtc
     1621 ctctactcgc tcgggaactt cgttttcggg acgccgggcc gcttcggacc gtcggcgccc
     1681 ggctacggtt tggtcgcgcg ggtcgttctc gacggggacg gcatcgatcg cgtcgagctg
     1741 acgggcatcg tgacggacaa cgacatcgtc gcgttccggc cgcgcccttg cgatcccgcc
     1801 agctccgacg ccattctaga gtcgctcgat tcgcccactg ccgcgttctg cggcacggca
     1861 ctcatcgaat ggccgcgcgg gacaaccttg ccctccgccg cgactcgcgc tactcgaccg
     1921 tgaactcggc caccatcccc ttgaacgcgt gcggcgtgcc ctccgggtcg tccgtatcgg
     1981 gaaggaagca aaccagcccg tagcgcccgg gctcgagggg agcaggcagc gcgacatccg
     2041 actcgtcgcc cggctcgtac gtggtccgca cgagaatggg ctccggctgg aagctctcgt
     2101 cctgcacgag ctcctcgatt gggcgaccct ccggcagggc aacgaggacg agttcgtgag
     2161 gctgctgccc ttcattcgtc acatgaaaag cgaagtcgcc gcttgtcacc gcgggcgatt
     2221 caagatcgcc attgaaagcg aactccgcga gcccgatctc gacaacctcc gtcccggacg
     2281 gtatgtcgtc attcggaacg aacagccggt cagccttcca ttcgtcgccg tccttgatga
     2341 ggccgacccc gaacgtgatt ccgtcgccct cgagggtcag tgtggcagaa tcaccctcga
     2401 tcatcacggt
//
LOCUS       DATMBB010000156         2774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_442670, whole genome shotgun sequence.
ACCESSION   DATMBB010000156 DATMBB010000000
VERSION     DATMBB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442670"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VNL92_03300"
     CDS             complement(<1..574)
                     /locus_tag="VNL92_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWO93768.1"
                     /translation="MRAIGYFRELASDEAGGESIARQNDAFLKFCKRQGYEAAATFLD
                     SASSNGDRPGLRQLLSYLRRPEKGFVVIVVAGLDRLGDSPRDAARAFFQIESLGTQLV
                     MMDQPGDSLAQLLDAWGAAQAREHVGDRVREAMRRKAVRGEVLGRPPYGYRVGLRRKL
                     ELVPEEAAIVRYVFRLYLHEGLGIRLIVQRM"
     gene            complement(683..1630)
                     /locus_tag="VNL92_03305"
     CDS             complement(683..1630)
                     /locus_tag="VNL92_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWO93769.1"
                     /translation="MLNFLLQAAPKPYFYGGQAVLEGVMMRGARSMAVAVRRQNGEIV
                     VHEETLSGPYAGTVRNIPLVRGVLTLAETLTLGVRALLFSTNVALEDEGEEELGSGAV
                     AGMLTFSLAFIAGIFFIAPLVAVGLLDRVWDGGGHTTNVIIEGVLRLGLFFAYVWAIG
                     LMPDIRRTFAYHGAEHRAIHCWEAGDSLTVRNVLRFPNEHPRCGTGFLLVVMVVAIGV
                     FAFVGDLPWWGLVLSRILLIPVIAAIAYEIVRFSAAFWHHRLVRAVFTPSLRLQAFTT
                     RDPDADQVEVAIAALSAVLAADGVAASRSTETHFSPAVT"
     gene            1786..>2774
                     /locus_tag="VNL92_03310"
     CDS             1786..>2774
                     /locus_tag="VNL92_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWO93770.1"
                     /translation="MFTHLHLHSEYSLLDGLGRIPGILDRVAADGQVACALTDHGVLY
                     GAIDFYREAKARGLNPIIGVEAYVAPGSRLQRDQEAKNPYHLTLLAKNRTGYSNLLKL
                     VTSANLEGYYYKPRIDRDLLAQHHEGLIVLSGCPSSEVHRALQADREDDARAIASWYK
                     DVFGEDYFIEVQRHGNLDEYNRLNPKLVDLARWLGSPVVATNDAHYVDRGDARFHDVL
                     LCIGTNATVNEANRMRFSDDSYYVTSEAEMRSLFDDLPEAIDNTMLVAERCELELDFD
                     RLHLPVPDIPDGLTPDEYLAQLTREGARRRYPDVDEAVQARIDYELGVVRQTG"
BASE COUNT          565 a          915 c          865 g          429 t
ORIGIN      
        1 tcattcgctg cacgatgagg cgaatgccca agccttcatg caggtagagc cggaacacat
       61 agcggacgat ggccgcctcc tccggcacga gctcgagctt gcggcgaaga ccgacgcgat
      121 agccgtacgg cggccggccc aggacctcgc cgcgcacggc cttcctgcgc atcgcctcgc
      181 gaacacggtc gcccacatgc tcccttgcct gcgcagctcc ccacgcatcg agcaactgag
      241 ccaggctatc acccggctgg tccatcatga cgagctgcgt gccgaggctc tcgatctgga
      301 agaaggcgcg cgccgcatcg cgcggcgaat caccaagacg atcgaggccc gcgacgacga
      361 tgacgacgaa ccccttctcc gggcgccgca aatacgatag aagctgccgc agacccgggc
      421 ggtcgccgtt cgacgaggcg gagtcgagaa acgtggcagc ggcctcgtag ccctggcgct
      481 tgcagaactt caggaaggca tcgttctgcc gcgcgatgga ttcgcctccc gcctcatcgc
      541 tggccagttc acggaagtag ccgatagccc gcatcgttgg ctccggttcg tactgtggag
      601 ggagatgcgc gtcgcgcgct gtggtcaggg gtcagtatag gaagggaaac aagccgcgac
      661 aagcagactt ggaacagctc gattacgtga cggccggcga aaagtgagtc tcagtcgacc
      721 tggacgctgc gacgccgtcg gccgccagga ccgcgctgag cgccgcaatc gcaacttcca
      781 cctggtccgc atcggggtcg cgcgtcgtga acgcctggag gcgcaaactc ggcgtaaaga
      841 cggcccgaac gaggcggtga tgccagaacg cagctgagaa ccgcacgatc tcatacgcga
      901 tcgccgcgat gacggggata aggagaatgc gggacaacac gagaccccac cacggcaggt
      961 cgccaacaaa ggcgaacacg ccaatcgcca ccaccatgac aacgagcaga aagccggtcc
     1021 cgcagcgcgg gtgttcgttg ggaaagcgca gcacgttgcg tacggtcagc gaatcgccgg
     1081 cctcccagca gtggatggcg cgatgctccg cgccgtggta ggcgaacgta cgccgtatat
     1141 cgggcatcag gccaatcgcc cagacgtatg cgaagaacag accgagccgc agaacgccct
     1201 cgataattac gttcgtggta tggccacccc catcccacac ccgatcgagc agcccgacgg
     1261 cgaccagcgg cgcgataaag aagatgcccg cgatgaacgc caaagagaag gtcagcatgc
     1321 cagccacggc gccgctgccg agctcttcct cgccctcatc ctccagggcg acattcgtcg
     1381 agaagagcag ggcgcggaca ccgagcgtca acgtctcagc gagggtgagc acgccacgca
     1441 cgaggggaat gttcctgacc gtgccggcgt acggaccgct gagtgtctcc tcatggacga
     1501 cgatctcgcc gttctggcga cgcacggcga cggccatgga gcgcgctccg cgcatcatga
     1561 ccccttcgag gacggcttga ccgccgtaga agtagggctt gggggcggcc tgcaggagga
     1621 aatttagcat tcgacgtgac cctcacaagg tgcgtgcttt agcagtgtat cgtcccggtg
     1681 gcgaaagcgg caacgcgaag atcctacgct aacgcgctgg tcttcacata actgcccttg
     1741 agcagctccg cccggggcgg gataattggc gatgaggcgc ccctaatgtt cacgcaccta
     1801 cacctgcatt ctgaatacag cctcctcgat gggctgggcc gcataccagg catcctcgat
     1861 cgcgtcgcgg ccgatgggca ggtggcttgc gcactcacgg atcacggcgt cctctacggc
     1921 gccatcgact tttaccggga ggcgaaggcg cgtggactca atcccatcat cggcgttgag
     1981 gcgtacgtcg cccccggatc gcgcctgcag cgcgaccagg aggcgaagaa tccgtaccat
     2041 ctgacactcc tggccaagaa ccgcactggc tacagcaacc tgctcaagct cgtgacgagc
     2101 gccaatctcg aaggctacta ctacaagccg cgcatcgatc gggacttgct ggcccagcac
     2161 cacgagggtc tcatcgtgct ctccggctgt ccaagcagcg aggtccatcg tgccctgcag
     2221 gcggaccgcg aggacgacgc gcgggccatc gcgagctggt acaaggacgt cttcggggag
     2281 gactacttca tcgaggtgca gcgtcacggc aacctcgacg agtacaacag gctgaatccg
     2341 aagctcgtag acctcgcccg ctggttagga tcgccggtcg tcgccaccaa cgatgcccac
     2401 tatgtcgacc ggggcgacgc tcgcttccac gacgtcctgc tttgcatcgg cacgaacgcg
     2461 acggtcaacg aggccaatcg catgcgcttc tccgacgact cgtactatgt gacgtcggaa
     2521 gcagagatgc gctccctctt cgacgacctc ccggaggcca tcgataacac gatgctcgtc
     2581 gctgagcgat gcgagctcga gctcgacttc gaccgccttc acctgccggt cccggacatc
     2641 cctgacggct tgacccccga cgagtacttg gcacagctca cgcgagaggg cgcacggcgg
     2701 cgttacccgg acgtcgacga ggcggtgcag gcgcgcatcg attacgagct cggcgtcgtg
     2761 cggcagacgg gttt
//
LOCUS       DATMBB010000157         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_358562, whole genome shotgun sequence.
ACCESSION   DATMBB010000157 DATMBB010000000
VERSION     DATMBB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358562"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VNL92_03315"
     CDS             complement(<1..690)
                     /locus_tag="VNL92_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWO93771.1"
                     /translation="MSTRIAVVGAGPGGYAAAFYAADLGMQVTLIDPEKNPGGVCLYR
                     GCIPSKALLHVAKVIDEAAHARDWGIAFDKPAIDINALRGYKNRVVEKLTAGTGQVAK
                     LRKVNYLQGWATLLSPTRLQVRTAGGEQIVDVDDIILATGSEPTRIPALSINSPRVLD
                     STSALDLPEIPKTLLVIGGGYIGLELGSVYAALGTRVSVVEMTDGLLPGADRDLVRLV
                     AQRVEKTFERVM"
     gene            complement(833..>2010)
                     /locus_tag="VNL92_03320"
     CDS             complement(833..>2010)
                     /locus_tag="VNL92_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWO93772.1"
                     /translation="STPAGEPSELQSSTPAGEPSEPQSSTPARERSERPVERSEPPVE
                     RSEQEERSDEGERKRAPVVDIRERQAAAPPQAAPTVPAVPSTASAPASPSVRRIAREI
                     GVDISQVTGTGAGGRITEEDVKNFARQILSSLGGGAIAPSPARPGGGPALPDFSKWGE
                     VERKPMSGVRRKTAEHLSHAWLAIPHVTQFDKADMTVLEEIRPKYAGEVERQGGKLTV
                     TAIITKIVATALRKFPQFNASIDLATSSIVYKKYVHIGIAVDTEHGLLVPVVRNADQK
                     NIVQIAVEIHQLAEKAKARKLSLDEMSGGSMSITNLGGIGGTAFTPIVNWPEVAILGI
                     SRGAVEAVFKDGQIVPRQMLPLSLSYDHRVIDGADAIRFLRWVVDAIEQPFTVMLRG"
BASE COUNT          335 a          647 c          634 g          394 t
ORIGIN      
        1 catgacgcgc tcgaaggtct tctccacgcg ctgcgcgacc agacgcacga gatcgcgatc
       61 ggcgcccggg agcaggccat cggtcatttc gaccactgac actctcgtgc ccagggctgc
      121 gtacacggag ccgagctcga gaccgatgta gccgccgccg atcacgagaa gggttttcgg
      181 gatctcgggc agatcgagcg cgctcgtgga atcgagcacg cgcggactgt tgatcgacaa
      241 cgccgggatt cgcgtgggct ccgacccggt cgccaggatg atgtcgtcga cgtcgacgat
      301 ctgctcaccg cccgcggtgc gcacctggag gcgcgtcggg ctcaacagcg tcgcccagcc
      361 ttgcaggtag ttcaccttgc gcagcttcgc cacttgaccg gtgccggcag tgagcttctc
      421 gacgacccga ttcttgtagc cgcgcagcgc gttgatgtcg atggccggct tgtcgaaagc
      481 gatgccccaa tcgcgcgcat gcgccgcctc gtcgatcact ttcgcgacgt gcagcagcgc
      541 cttggaagga atgcacccgc ggtagagaca cacgccgccg ggattcttct ccggatcgat
      601 cagcgtgacc tgcatcccca aatcggccgc gtagaaggcg gccgcgtatc cgcccggacc
      661 ggcaccgacg actgcgattc gtgtggacat aatgaagcgt atggcgaggc atgccttgta
      721 cggattaagc agattatcta aatgaccttt aatgaaatct gcgtaatcag cctcatctgc
      781 ggataatcaa atctgcgtaa ttggttcgcc tgtgcgaaat ccgttcgccc gtttaccccc
      841 gcaacatcac ggtgaacggc tgttcgatag cgtcgacgac ccagcgcagg aatcgaatcg
      901 catcggcgcc gtcgatcacc cggtgatcgt agctcagcga caacggaagc atctgtcgcg
      961 ggacgatctg tccgtccttg aacacggctt cgacagcgcc tcgcgagatc ccgaggatgg
     1021 cgacttcagg ccagttgacg atgggagtga aggcggtgcc gccaatgccg ccgagattgg
     1081 tgatcgacat cgagccgccg gacatctcgt cgagcgagag cttgcgggct ttggcttttt
     1141 ccgcgagctg atggatctcg acggcgatct gcacgatgtt cttctgatcg gcgttgcgca
     1201 ccactggtac aagcaggccg tgctcggtgt ccacggcaat cccgatgtgc acgtatttct
     1261 tgtagacgat cgagctcgtc gcgagatcga tcgacgcgtt gaactgcggg aacttgcgca
     1321 gcgccgtggc gacgatcttc gtgatgatgg cggtgaccgt gagcttgcca ccctggcgtt
     1381 cgacctcgcc cgcgtacttc ggccggatct cctcgagcac cgtcatgtcg gccttgtcga
     1441 actgcgtcac gtgcggaatc gccaaccaag cgtggctgag gtgttcggcc gtctttcgcc
     1501 ggacaccgct catcggcttg cgttcaacct cgccccattt gctgaagtcg ggcagtgcgg
     1561 gcccgccgcc tggacgggcg gggctcggag cgatcgcgcc gcctccaagg ctgctcagga
     1621 tctgacgcgc gaaattcttg acgtcctctt cggtgatgcg gccgcctgct cccgtgccgg
     1681 tgacctggct gatatcgacg ccaatctcgc gtgcaatgcg ccgcaccgag ggcgacgccg
     1741 gcgcagacgc ggtgctcggc acggcaggca ccgtcggcgc ggcctgtggt ggcgcagccg
     1801 cttgacgctc tcggatatcg acgactggcg ctcgcttcct ttcgccctcg tcgctccgct
     1861 cctcctgctc gctgcgctca acaggtggct cgctgcgctc gacaggtcgc tcgcttcgct
     1921 cgcgtgcagg cgtcgaagac tggggctcgc ttggctcgcc tgcaggcgtc gaagactgga
     1981 gctcgcttgg ctcgcctgca ggcgtcgaag
//
LOCUS       DATMBB010000158         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_69923, whole genome shotgun sequence.
ACCESSION   DATMBB010000158 DATMBB010000000
VERSION     DATMBB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69923"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VNL92_03325"
     CDS             complement(<1..750)
                     /locus_tag="VNL92_03325"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWO93773.1"
                     /translation="MSNPRDRLRRILAEEQRRGYRDDTVTGGLDAFLSRMSAGEDREL
                     RRALLVLPPEGYRGLTPGKRRDWADLALGHAARTRSVAAEGPGHGTRLPPPNPGRRAS
                     RPAASRRRVSGPARVAPSQVALETPVEGLPGIRSDIAKKLASLGVRTLRDLLLFFPHR
                     HADYAEMHSIGALRPGEDTTVVGEVWSAAEAQIGRRMKGTEAVVADETGTLRVVWFNQ
                     PWIAKQLRTGQRIVLSGRASAFRGRLVMESPE"
     gene            complement(747..1592)
                     /locus_tag="VNL92_03330"
     CDS             complement(747..1592)
                     /locus_tag="VNL92_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019533666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWO93774.1"
                     /translation="MPIRVVTDSTADVPNDLAEELGIAVVPLYVNFGDDTFRDGVDMS
                     TDDFYERLVASKVLPTTSQPSAGDFEDVYRKLAEETTEIVSIHLSGKLSGTLGSAATA
                     AAAVPECTIEVIDSRSASLGCGLAAIKAAQAARAGARMHEVLDLVQHVTDHQRVVFTV
                     DTLEYLRRGGRIGRGAAFLGSLLSIKPLLELRDGEIFPVERVRSRKKALNRMYEGLSN
                     LEAISDIGIIHATTPDDADEMRARLEERFPDVRFWTGRIGPVLGAHTGPGILGVLALR
                     RRGEE"
     gene            1882..>2217
                     /locus_tag="VNL92_03335"
     CDS             1882..>2217
                     /locus_tag="VNL92_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93775.1"
                     /translation="MTTEQTAGTELLPGREALDHAAERIEELLEGDQEQAAAQALAQL
                     HPADEADIVRDIEPALRARLLPHIEAESLAHIIEQLDGRDRGRLVNQLPRPTLARVLD
                     RTDSDVAADV"
BASE COUNT          348 a          736 c          768 g          365 t
ORIGIN      
        1 ctccggcgac tccatgacga gacgcccgcg gaaggccgaa gcccgcccgg acaggacgat
       61 gcgctgaccc gtacgcagct gcttcgcgat ccatggctgg ttgaaccaga cgactcgcag
      121 cgtcccggtc tcgtcggcca cgacggcctc cgtgcccttc atacgccggc cgatctgcgc
      181 ttccgcggcc gaccacacct cgccgacgac cgtggtgtct tcgcccggcc ggagcgcgcc
      241 gatggagtgc atctccgcat agtccgcgtg gcggtggggg aagaacagca gtaggtcgcg
      301 gagcgtccgc actccgagcg atgcgagctt cttcgcgatg tcgctccgga tccccgggag
      361 tccttccacc ggcgtctcga gcgccacctg cgatggtgcg acgcgagcag ggccggagac
      421 gcgccgtcga cttgcggccg ggcgggaggc tcgccggccg gggtttggag gtgggaggcg
      481 tgtgccgtgg cctgggcctt cggcagcgac cgatcgcgtg cgggctgcat ggccaagggc
      541 gaggtcggcc cagtcgcgcc gcttgccggg agtcagccct cgatagcctt ccggaggcag
      601 gacgagcaga gcgcgccgca actcgcggtc ctcgcccgcc gacatgcggg agaggaaggc
      661 atcgaggcca cccgtgacgg tgtcgtcgcg gtacccgcgc cgctgttcct ctgccagaat
      721 gcgccgcaat cgatctcttg ggttgctcac tcctcccctc gccggcgcag cgcgagcacg
      781 ccgaggatgc caggacctgt gtgggctccg agcacgggac caatgcgccc cgtccagaat
      841 cggacgtccg ggaagcgctc ttccagccgg gctcgcattt cgtcggcgtc atccggtgta
      901 gtggcgtgga tgatgccgat atcggaaatg gcctccaggt tggaaagccc ctcgtacatg
      961 cgattcagcg ccttcttccg gcttcggacg cgctctacgg ggaagatctc accatcgcgc
     1021 agttcgagca acggcttgat cgagagcagg gagccgagga acgcggcgcc ccgtccgatg
     1081 cggccgccgc gtcgcagata ctccagcgtg tcgacggtga acacgacgcg ctggtgatcg
     1141 gtgacgtgct gtacgaggtc gagcacctcg tgcatgcggg cgcccgcacg ggcggcctgt
     1201 gcggccttga tcgcggcgag cccgcacccg agcgaggcgc tgcgcgaatc gatgacttcg
     1261 atggtgcatt cggggacggc tgccgccgcc gtcgccgccg agcccagcgt accggagagc
     1321 ttgcccgaca ggtggatgga gacaatctcg gtggtctcct ccgcgagctt gcggtacaca
     1381 tcctcgaaat cgccggcgct cggctgggag gtggtgggga ggaccttcga cgccacgagc
     1441 cgttcgtaga aatcgtcagt tgacatgtcg acgccgtcac ggaacgtgtc gtctccgaag
     1501 ttcacgtaca gcgggactac ggcgatgccc aactcctcgg cgaggtcgtt tgggacgtcg
     1561 gccgtgctgt cggtcaccac tcgaatcggc atgacgtcct ccgttggtgg cgagtataga
     1621 ccagccgagc gggaaagcga acctcgagca ggtcctatgc gatcctagca gttcctcgcc
     1681 acgcctcgca cccgtgcgag cgcgttgacg taccgtgcgc ggcggcgata cgctgaacat
     1741 ccgggcggcc tgcgcccggg gaggacggtt aggtgtagac agagcaagcg tgcctcgaga
     1801 tgtccgcggt tcgtgccgcg cccgcttgct ctgacccttc cgaataacgg aagggttgtt
     1861 cttgttcggt agggcgtgtc gatgacgacg gaacagacgg ctggcacgga gctcttgccg
     1921 ggacgagagg cgctcgatca tgcggcggag cggatcgagg agttgctcga gggcgaccag
     1981 gagcaggctg cggcccaggc tctggctcag ctgcacccgg ccgacgaggc cgatatcgtt
     2041 cgcgatatcg agcccgcgct tcgcgctcgc cttctcccgc acatcgaggc ggagtccctt
     2101 gcccacatca tcgagcagct cgatggccgc gatcgcggcc gcctcgtgaa ccagctaccg
     2161 cgaccgacac tcgcgcgggt cctcgatcgc accgattcgg acgtcgccgc ggatgtc
//
LOCUS       DATMBB010000159         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_402045, whole genome shotgun sequence.
ACCESSION   DATMBB010000159 DATMBB010000000
VERSION     DATMBB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402045"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VNL92_03340"
     CDS             complement(<1..290)
                     /locus_tag="VNL92_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93776.1"
                     /translation="MEAIAVVLALALIITLVALLAATRRPASSPSFEAGRIVEQLQSL
                     QAQLAPLPGIRSHLDVDGRLWEEMSRRIEETQRAIDALRLQAEERRRRDEDLV"
     gene            413..700
                     /gene="gatC"
                     /locus_tag="VNL92_03345"
     CDS             413..700
                     /gene="gatC"
                     /locus_tag="VNL92_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWO93777.1"
                     /translation="MSLTREEVLHLAKLARLGLSEAEVEMLRQQLSQILDYFAMLNEI
                     DTSEVPPTMHAIPMQNVTREDVPRPSLQREAVLANAPQAEEGYIRVRAVLE"
     gene            721..2199
                     /gene="gatA"
                     /locus_tag="VNL92_03350"
     CDS             721..2199
                     /gene="gatA"
                     /locus_tag="VNL92_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWO93778.1"
                     /translation="MDPYQLSISEASDLLQRREISAVELTTSVLERIATTEAALGAYV
                     AVTEDLAREQAVQAEARRSRGEAEALTGIPICHKDVLATAGVPTTCGSRMLERFVPPY
                     DATVVSRMKAAGVVMVGKGNMDEFAMGSSTENSALHPTRNPWDLSRVPGGSSGGSAAA
                     VAAGQALGATGSDTGGSIRQPAAFCGITGLKPTYGRVSRFGLVAFASSLDQVGPLART
                     ARDCAMLLQAMAGHDPNDSTSADIPVPDYVAALTGEVRGLRIGVPSEYFPDDLDANVR
                     SAIEEALAVFEHAGAQIVRDISLPSTRYAVATYYILAPSEASANLARYDGVKYGYSYQ
                     DGGGMWENMEKTRELGFGSEVKRRIMLATYALSAGYYDAYYLRAQRVRTLIRREFEAA
                     FQRCDVLLGPTTPTVAFPLGAKIDDPLQMYLNDVFTIPVNIAALPGISIPCGFVEGLP
                     VGLQLIGRAFDEETLLRAADAYQRLTDWHQRRPDVRVEEISA"
     gene            2196..2447
                     /locus_tag="VNL92_03355"
     CDS             2196..2447
                     /locus_tag="VNL92_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWO93779.1"
                     /translation="MSEDGIIGGIERSPEQAVHDVLDMLERDARLAPERIAQAIGRSA
                     DEVRDIIAQAEEDRIILHYGALIDWSRAGESEVEPLRYA"
BASE COUNT          477 a          906 c          848 g          421 t
ORIGIN      
        1 acgaggtcct cgtcccggcg acgccgttcc tccgcctgca gacgcagcgc gtcgatagca
       61 cgctgcgtct cctcgatgcg acgcgacatc tcctcccata gccgtccatc tacatcgaga
      121 tggctgcgaa tccccgggag gggcgccaac tgagcctgca agctctgcaa ctgctcgacg
      181 atcctcccgg cctcgaacga ggggctggac gccggccttc gcgtcgccgc gaggagagcc
      241 accagcgtaa tgatgagcgc gagggcgagg acaacggcaa tcgcctccat atgcagcctc
      301 cagtccagca catttgtgct gtcgaccccg attctacccg ccggtgccgc tttcgaacaa
      361 gccgggcttc gctatcatcg aagggttgct atactcccga aggaggtacg cgatgtccct
      421 gacgcgggag gaggtgctgc acctggcgaa gctcgcccgc ctcgggttga gcgaggcgga
      481 agtcgagatg ctccgccaac agctgtcgca gattctcgac tactttgcga tgctcaatga
      541 gatcgatacg tccgaggtac ccccgaccat gcatgccatc cccatgcaaa acgtcacgcg
      601 ggaggacgtc ccccgcccgt cgctgcaacg cgaggccgta ctggcaaacg cgccgcaggc
      661 cgaagagggc tacatccgcg tccgcgcagt cctcgagtag tcccacaggg gagacgagca
      721 ctggacccct atcaactgtc gatctccgag gcctcggacc tcctgcaacg tcgcgagatc
      781 tcggccgtcg agctcaccac cagcgtgctc gagcgtatcg cgacgacgga ggccgcgctc
      841 ggcgcctacg tcgcagtcac cgaggacttg gcgcgcgagc aggccgtcca ggctgaggcg
      901 cgacgatccc gcggcgaagc ggaggccctg acgggcatcc ctatctgcca caaggacgtt
      961 cttgccacag ccggcgtgcc cacgacgtgc gggtcgcgca tgctcgagcg cttcgtccct
     1021 ccgtacgacg ccaccgtcgt gagccggatg aaggcggccg gggtggtcat ggtcggcaag
     1081 ggcaacatgg acgaattcgc catgggctcg agcaccgaga actccgccct tcacccaaca
     1141 cgaaacccct gggacctgag ccgcgtcccc ggcggctctt ccggtggctc ggcggcagcg
     1201 gtcgcggccg gccaagcgct gggcgccact ggctccgata ctgggggcag cattcgtcag
     1261 cccgctgcgt tctgcggcat caccggcctc aagccaacat acgggcgtgt ctcccggttc
     1321 ggactcgtcg cgttcgccag ctcgctcgac caggtgggcc ccctggcgcg aacagcccgc
     1381 gactgcgcaa tgctgctgca agccatggcg ggccacgacc ctaacgactc gacgagcgct
     1441 gatatccccg tgcccgatta cgtggccgcc ctgacggggg aagttcgcgg tctccggatc
     1501 ggcgtgcctt ccgagtactt ccccgacgac ctcgatgcaa acgtgcgctc ggccatagag
     1561 gaggccctcg cggtcttcga gcacgcaggc gcgcagatcg tgcgcgacat atcactccca
     1621 agcacgcgct atgccgtcgc aacgtactac atcctcgccc caagcgaagc ctcggcgaat
     1681 ctggcgcgtt atgatggcgt gaagtacggt tactcctacc aggacggcgg cggtatgtgg
     1741 gagaacatgg agaagacgcg cgagctcggc ttcggcagtg aggtgaagcg tcgcatcatg
     1801 cttgccacct acgcgttatc ggccggctac tacgacgcgt attacctccg ggcacagaga
     1861 gtgcgcaccc tcatccggcg cgagttcgag gcagcgttcc aacgctgcga tgtccttctc
     1921 gggccgacga cgcccaccgt ggcgttcccg ctgggcgcga agatcgacga cccgctgcag
     1981 atgtacctca acgacgtgtt caccatccca gtgaacatcg cggcgctgcc cggaatatcc
     2041 ataccatgcg gtttcgtgga aggcctgccg gtgggcctcc agctcatcgg gcgcgccttc
     2101 gatgaggaga cgttgcttcg agctgccgat gcgtaccagc gcctgaccga ctggcatcaa
     2161 cgacgcccgg atgtccgcgt cgaggagata tcagcatgag cgaggacggc atcatcggag
     2221 gcatcgagcg ctcgccggag caggccgtgc acgacgtgct cgacatgctg gagcgggacg
     2281 ctcgcctcgc tcccgagcgc atcgcccagg ccatcggccg cagcgccgac gaggtgcgcg
     2341 acatcattgc ccaggccgag gaagatagga tcatcctgca ctacggagcg ctcatcgact
     2401 ggtcgcgcgc cggcgagagc gaggtcgagc ccctccgata cgcctgacgt gatgaggatt
     2461 tcgctttccg ggtcgtactc gatgccgtag tgccggcgga tggagcgcga gagggcgtga
     2521 cgaaatcggg ctcgccgacg ccgagcgaga tgacgccgtc catcgaggac agcaagtcga
     2581 agaagcgccg gatgccggac gcgggaaggt ctgccacccg ttgcgagatc gcgccgcgcc
     2641 tcgccgcgag gc
//
LOCUS       DATMBB010000160         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_338186, whole genome shotgun sequence.
ACCESSION   DATMBB010000160 DATMBB010000000
VERSION     DATMBB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338186"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VNL92_03360"
     CDS             <1..609
                     /locus_tag="VNL92_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HWO93780.1"
                     /translation="VITLDVPERAWDVAALLQNMDRYGVEAIHSRFRQSEQVVSVAAT
                     RLHNIAGKYTGKDDPIAIVRTQGIFPAPEEVTEPYLIAHYVTGDARGSHTMPLFPIAV
                     NTPVTGPYCHPLVSCLALSMDEKGRFSSNVVDAFDSPVWDEARRRSQEKALEMRKQGF
                     FGAAMASVGEMEYTGIVETYKALESEFNIRADGASPQAVAAR"
     gene            614..1234
                     /gene="rdgB"
                     /locus_tag="VNL92_03365"
     CDS             614..1234
                     /gene="rdgB"
                     /locus_tag="VNL92_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWO93781.1"
                     /translation="MPPTLLLATNNAGKQREFRELLADCGWELVVPAALRITLDAEEN
                     GRTYAENATIKAVAAAKAAKIPALADDSGLEVDALHGAPGLFSERYAGPGATDADRRA
                     KLLRELERVPEPSRTARFRCAIAIAVPEGSAVHVSVHEGEVQGVILMHERGEGGFGYD
                     PLFLIPELGRTMAELRPEEKNEVSHRGRAARSACIELRSIAAPLDR"
     gene            1261..2274
                     /gene="moaA"
                     /locus_tag="VNL92_03370"
     CDS             1261..2274
                     /gene="moaA"
                     /locus_tag="VNL92_03370"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWO93782.1"
                     /translation="MSVTIRDTLARPLRDLRISVTDRCNFRCTYCMPKEVFGRNYQFL
                     PSDRILSFEEIARLARIFVDHGVEKIRVTGGEPLVRRDIEHLVAMLAEIEGLRDLTLT
                     TNGSLLAQKARALRDAGLRRITVSLDSLDGGVFRAMNDVDFPVKKVLEGIDAAASAGL
                     APIKVNMVVKRGVNDGDLLDMAAYFRGSGHILRFIEYMDVGHTNGWRMDDVVPAEEIV
                     RTIDAVFPIEPAEPNYRGEVARRWRYRDGMGEIGIIASVSQPFCGNCTRARLSPEGQL
                     YTCLFGTHGHDFRALLRDGASDEDLAEAVRSVWSMRADRYSQLRSAETVALPKVEMSY
                     IGG"
     gene            2278..2751
                     /gene="moaC"
                     /locus_tag="VNL92_03375"
     CDS             2278..2751
                     /gene="moaC"
                     /locus_tag="VNL92_03375"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWO93783.1"
                     /translation="MSLSHLDDQGRAQMVDVTAKADTHRVAEAAGHIVMRPETLALIQ
                     TGGVPKGDVLAVARVAAVMAAKRTAELIPLCHPLPLTGIEVELRPNEAEACVDIHVVV
                     RTTGKTGVEMEALTAVSVAALTIYDMCKAVDRGMRIRDVRLLRKAGGKSGEIVLE"
     gene            complement(2764..>2988)
                     /locus_tag="VNL92_03380"
     CDS             complement(2764..>2988)
                     /locus_tag="VNL92_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93784.1"
                     /translation="PEYGCIVVKEDGELYEFRMGMDFSAGDDLVAVRNEETRPIALHP
                     RDYIVYAYNGLVALTELLLEQEEAGQQTSP"
BASE COUNT          537 a          952 c          989 g          510 t
ORIGIN      
        1 gtcatcacgc tcgatgttcc ggagcgcgca tgggatgtgg cggcgctgct gcagaacatg
       61 gaccgctacg gcgtcgaggc catccactca cgcttccggc agagcgagca ggtcgtttct
      121 gtggcggcga ccaggctgca caacatcgcg gggaagtaca ccgggaaaga tgatccgatc
      181 gccatcgttc gaacgcaggg aatcttcccg gcaccggaag aggtgacgga gccctacctc
      241 atcgcgcact acgtgaccgg cgatgcccga ggttcgcaca cgatgccgct cttcccgatc
      301 gcggtgaaca cgccggtcac agggccgtac tgccacccgc tcgtgtcgtg cctcgcgctt
      361 tcgatggatg agaagggacg gttctccagc aacgtcgtcg atgccttcga tagccctgtt
      421 tgggacgagg cacgccgacg gtcgcaggag aaggcgctcg agatgcgcaa gcagggcttc
      481 ttcggcgcgg cgatggcctc cgtcggcgag atggagtaca cgggcatcgt ggagacgtac
      541 aaggcgctcg agtccgagtt caacatcaga gccgacggcg cgagcccgca agctgtcgcc
      601 gcgcggtagc agcgtgccgc ccacgctgct gttggcgacc aacaacgccg gcaagcagcg
      661 ggaatttcga gagctcctgg ccgattgtgg ctgggagctc gtcgttcctg cagcgctccg
      721 gattaccttg gatgccgagg agaacggtcg aacctacgcg gagaatgcta caatcaaagc
      781 agtggcagcc gcgaaggcgg cgaagatccc ggcgctcgcc gatgactctg gcctggaggt
      841 cgacgcattg catggggcgc cgggtttatt ctcggagcga tatgctggcc cgggcgcaac
      901 ggatgccgat cgccgcgcaa agctgctgcg agaactggag cgagtgcccg agccgtcgcg
      961 aacggcgcga tttcggtgcg cgatcgctat cgcggttccg gaggggtccg ccgtccacgt
     1021 gtcggtccac gaaggcgaag tccagggggt catcctgatg cacgaacgcg gcgaaggagg
     1081 cttcggctac gatccgctct ttctcatccc cgaactcggc cgaaccatgg ccgagttgcg
     1141 gccggaggag aagaacgagg tcagccaccg gggccgagcc gcccgttccg cgtgcattga
     1201 gctgcggtct atcgccgcgc cgctcgaccg ctgattcgaa gctgggggac cagagtcacg
     1261 atgtctgtca caatccgcga cactttggcg cgaccgttgc gcgacctgcg catatcggtc
     1321 acggaccggt gcaacttccg ctgcacctac tgcatgccca aggaagtctt cggacgcaac
     1381 tatcagttcc tgccatccga ccgcattctc agcttcgagg agatcgcacg gctcgcccgc
     1441 attttcgtag accacggggt cgagaagatt cgcgtgacgg gtggcgagcc gctcgttcgg
     1501 cgcgacatcg agcacctcgt cgcgatgctc gcggagatcg aagggctccg cgacctgacg
     1561 ctgaccacga acggctcact gttggctcag aaggcgcggg cgctccgcga tgccggcctg
     1621 cgccgcatca cagtgagcct cgattcgctc gacggtggcg tcttccgtgc catgaatgat
     1681 gtcgacttcc ccgtgaagaa ggtgctcgaa ggcatcgacg ccgcggcctc cgccggactt
     1741 gcgccaatca aggtcaacat ggtggtcaag cgcggcgtga acgatggcga ccttctcgac
     1801 atggccgcct acttccgcgg cagcggccat atcctgcgct tcattgaata catggacgtg
     1861 ggccacacga atggctggcg catggatgac gtcgttccgg ccgaggagat tgtgcgaacc
     1921 atcgatgccg tcttcccgat cgagcctgcc gagccgaact atcgcggcga ggtcgcccgg
     1981 cgctggcgct atcgcgacgg catgggcgag atcggcatca tcgcctccgt cagccagccg
     2041 ttctgcggca actgcacacg agcgcggctc tcccccgagg gccagctgta cacctgcctc
     2101 ttcggcaccc acggccacga ttttcgagcc ctgctgcgcg acggagcgtc ggatgaagat
     2161 ctggcggagg ccgtgcgctc ggtctggagc atgcgcgccg ataggtactc gcagctgcgt
     2221 tccgccgaga ccgttgcgct ccccaaggtc gagatgtcct acattggggg atgattcatg
     2281 agcctctccc acctggatga tcagggccgg gcccagatgg tcgacgtcac ggccaaggcc
     2341 gatacacatc gtgtcgcgga ggccgccggc catatcgtca tgcggccgga aacgctcgcg
     2401 ctgatccaga cgggaggtgt gcccaagggc gacgtgctag ccgtggcccg cgtggccgcc
     2461 gtcatggccg cgaagcgcac cgccgaactc attcccctgt gccacccgct gccgctcacg
     2521 ggcatcgaag tggaactgag gccgaacgag gcggaggcgt gcgtggacat ccacgtcgtg
     2581 gtgcgcacaa cgggcaagac gggcgtcgag atggaggcgc tgaccgccgt ttcagtggcg
     2641 gcgctcacga tctacgacat gtgcaaggcg gtcgataggg gcatgcggat ccgggacgtg
     2701 cgccttctcc gcaaggcggg cggcaagagc ggcgaaatcg tcctcgagtg atgatcgagg
     2761 acgctacggc gaggtctgct gccctgcttc ctcctgctcg agcagcagct ccgtgagagc
     2821 aacgagcccg ttgtaggcgt agacgatgta atcgcgcgga tgcagcgcta tcgggcgtgt
     2881 ctcctcgttc cgcacggcga cgaggtcatc gccggccgag aagtccatcc ccatccgaaa
     2941 ctcgtacaac tcgccgtcct ctttgacgac gatgcaaccg tattcggg
//
LOCUS       DATMBB010000161         3334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_151392, whole genome shotgun sequence.
ACCESSION   DATMBB010000161 DATMBB010000000
VERSION     DATMBB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151392"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..462
                     /locus_tag="VNL92_03385"
     CDS             169..462
                     /locus_tag="VNL92_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018933788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWO93785.1"
                     /translation="MAYVITEACVDVKSGDCQDVCPVDCIFTSPDDPQFYINPDLCID
                     CAACEPACPVNAIYFEDDVPDDMKQYIEINRRYFEEHSDEISTGFRRRTERTH"
     gene            602..1573
                     /locus_tag="VNL92_03390"
     CDS             602..1573
                     /locus_tag="VNL92_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWO93786.1"
                     /translation="MDIGQVEAFVQVANHRSFSKAAEALFLTQPSVTARIQSLEKELD
                     EEMFERTGRGVRLTEAGSVFLPYAEMLLRTLRDGRDALDSLRNAEFGNLRLGTAFTIS
                     AYVLPGILRNFCARYPGVEVSVRTGRSEQVLQMVLADDVQVGLVRALQHPEIESIPLH
                     EEEIVLVVNPSHEFAEKWIVGIDELARQPLIFFDKGSSYYALTQSLFRQAGVVPRIAM
                     ELDSMEATKKMVEEGLGIALLPRASVQREIELGILKEAQVRGAERVRRPISLIYRRNR
                     KRPRVVQAFMEVLAEMYGFRVPEAAKLSPRRRESSNGRPPAVPSRTA"
     gene            1688..1990
                     /locus_tag="VNL92_03395"
                     /pseudo
     CDS             1688..1990
                     /locus_tag="VNL92_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(2018..3049)
                     /locus_tag="VNL92_03400"
     CDS             complement(2018..3049)
                     /locus_tag="VNL92_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003921139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWO93787.1"
                     /translation="MPTAFVHDPLYELHDAGRNHPERPDRIRAALALLQEAPVFADLV
                     PLTARDATREELALVHDAALIDLVREASRAGDSWLDPDTHAGTHSFDAAVRSAGGCLA
                     AVDWVLDKAGRNAFALVRPPGHHATPTRAMGFCFFNNIAVAAAHALERRGLDRVAIVD
                     LDVHHGNGTEDCFASDPRVLYVSTHQYPFYPGTGALEDTGLGEGRGSTINVPLPAGSG
                     DVAYCEAMAQIVTPALQRFGPQLILVSVGFDAHFADPLAGMQLSNAGYAHMVASLCAL
                     SGELCEGRIVFALEGGYDLDAVAWGVLNTCEALAGLEPSPDPLGPAPPAPEPDIARLL
                     QRAKALHRL"
     gene            complement(3102..>3334)
                     /locus_tag="VNL92_03405"
     CDS             complement(3102..>3334)
                     /locus_tag="VNL92_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93788.1"
                     /translation="FGLAGEFFDDIAEVLPGANVDTVLGANGAIKGTPVPEVTEPGPF
                     GLWGSTAILGGYIAAFTTLAVVLFQRRDITSE"
BASE COUNT          584 a         1024 c         1162 g          564 t
ORIGIN      
        1 ctgccctggg acgatcgtga ggcggcgcgg cgggccctgg catcatgatc agcttatgta
       61 ggcattgata tagcgtgcgg ggttggcgag ttcagcatcg gtgtgtacta tggtggagcg
      121 aagcgatccc atcgcgggcc ggcgcccgaa ggatgaaggg gaagtccgat ggcttacgta
      181 atcactgaag catgtgtgga tgtaaagtcc ggcgattgcc aggatgtgtg cccggttgac
      241 tgcatcttca cttcgccgga tgatccacag ttctacatca acccggacct gtgtatcgac
      301 tgcgcggcgt gcgagccggc atgtccggtg aacgcgatct acttcgagga cgacgtgccg
      361 gacgatatga agcaatacat cgagatcaat cgccgctact ttgaagagca ctccgatgag
      421 atatcgaccg gttttcggcg gcgtacggag cgtacgcatt aagcgatcgg cgtcgcgaca
      481 tgagggcggg tccttcgggg tccgcccttt gctttgaccc gcggtaggtt cgtcgtgcta
      541 caatcgcgcc cgccggcgcc gctgggcggg tgtggcgcgg ccgagcggga gtggctggaa
      601 catggacatc ggacaagtcg aagcattcgt ccaggtcgcg aatcaccgaa gcttcagcaa
      661 ggcggcggag gcgcttttcc tcacgcagcc ctcggtgacg gcgcgcattc aatcgctgga
      721 gaaggagctc gacgaggaga tgttcgagcg cacggggagg ggggtgcgcc ttacggaggc
      781 tggcagtgtc tttctcccct atgccgagat gctgctgcga accttgcgcg acgggcgcga
      841 cgcgttggat agcctccgca acgcggaatt cgggaacctg aggctgggca cggccttcac
      901 catcagcgcc tacgttctgc ctgggatcct gaggaacttc tgcgcccgct accccggtgt
      961 cgaagtttcg gttcgcaccg gccgttcgga gcaggtgctg cagatggtgc tggccgacga
     1021 cgtgcaggtc ggcctggtgc gcgcgctgca gcatcccgag atcgaatcga tcccgctgca
     1081 cgaggaggaa atcgtcctcg tcgtgaaccc ttcccacgag ttcgcggaga agtggatcgt
     1141 cgggatcgac gagctggcga ggcagccgct catcttcttc gacaagggat cgagttacta
     1201 cgcgctgacc cagagcctct tccgccaggc gggcgttgtt ccgcggatcg ccatggagct
     1261 cgattcgatg gaggcgacga agaagatggt ggaggagggg ctcggtatcg ccctgctccc
     1321 ccgcgcatcg gtccagcgcg agatcgagct cgggattctt aaggaggcac aggtgcgcgg
     1381 cgcggaacgg gtgcgccggc ccatctcgct catctaccgc cggaatcgga agcgtccccg
     1441 cgtggtgcag gcgttcatgg aggtgctggc ggagatgtat ggcttccgcg ttccggaggc
     1501 tgccaagctc tcgccgcgcc gtcgcgagtc atcgaacggt cgtccgccgg cggtcccatc
     1561 ccgcacggcc tgatgcgaac tcctctacga gcaatagacg cgcggcctcg gtagccagca
     1621 tactcctcgc atgcggaacg acgagacagt cctctactgg aaacgcggtt gaacgcagtg
     1681 cactcgagcg agggcggcgc tctcgcgcag tgggcaaagc ttcgtcgagc gtgagctctt
     1741 tcgcgaaccg ctgagccgcg atgagctcga ggcgctggcg cgtgagacgg gccatgcgcc
     1801 gaaggacctc gtcgcgtggc gcagcccgcg ggctcgtgcg ctcgacccgt ccgtgattga
     1861 cgacgaggcc gcgctcgacc tcatggcgaa cgagccctac cttatccgcc gtccattggt
     1921 gcgaagaggc tcgcgtctcg tcatcggagg ccagcccgat gacatcgagc gcttgttcgc
     1981 cgaggactag cgtgggcggc tcgacgagcg cccgccatca gagccggtgc agtgccttgg
     2041 cccgctggag tagccgcgca atatctggct ccggtgcggg cggtgccgga ccgagcgggt
     2101 cgggcgaagg ctcgaggcca gcgagcgcct cgcacgtgtt gaggacgccc caggcgacgg
     2161 catctaggtc ataccctccc tcgagagcaa agacgatgcg cccctcgcaa agctcgcccg
     2221 agagggcgca gagagatgcg accatgtgcg cgtaaccggc gttcgagagc tgcatccccg
     2281 ccagcggatc ggcaaagtgg gcgtcgaacc caaccgacac caggatcagc tgcggcccaa
     2341 agcgctggag ggcgggcgtg acgatctggg ccatcgcttc gcagtaggcg acgtcgccgg
     2401 acccagcggg gagggggacg ttgatcgtgc ttccacgccc ctcgccgagg cccgtgtctt
     2461 cgagggcgcc cgtgccggga tagaacggat attggtgcgt cgagacgtac aggacccgcg
     2521 ggtccgacgc gaagcagtcc tctgtgccat tgccgtggtg aacgtcgaga tcgacgattg
     2581 cgacgcgatc gaggccgcgt cgttcgaggg catgagcggc ggcaacggct atgttgttga
     2641 agaagcagaa ccccatggcg cgggtggggg tggcgtggtg gcccgggggc cggacgagag
     2701 cgaacgcgtt tcgccctgcc ttgtcgagaa cccaatcaac tgccgcgagg cacccgcccg
     2761 cggatcgaac ggctgcatcg aagctgtgcg tcccagcatg cgtgtccggg tcgagccagc
     2821 tgtcgcccgc acgcgaggcc tcgcgcacaa gatcgatgag cgccgcatcg tggacgagcg
     2881 cgagctcctc tcgcgtcgca tcgcgagccg taagcggcac cagatcggca aagaccggcg
     2941 cctcctggag cagggcgagc gcggcgcgga tgcggtccgg ccgttccgga tggttcctgc
     3001 cggcgtcgtg cagctcgtag agcggatcgt gaacgaatgc ggtcggcacg cttctatgat
     3061 aggccaccag cggcagcgct tcgcatccct tgccggcggc gctattcgga cgtgatatcg
     3121 cggcgctgga acaagaccac agcgagcgtc gtgaaggcag cgatgtagcc gcccaagatg
     3181 gccgtcgagc cccacaggcc aaatggaccc ggctccgtca cctcggggac gggcgtgccc
     3241 ttgatcgcgc cgttcgcgcc aaggactgtg tcgacgttcg cgccgggcag cacttcggcg
     3301 atatcatcga agaactcgcc ggcgaggccg aata
//
LOCUS       DATMBB010000162         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_526502, whole genome shotgun sequence.
ACCESSION   DATMBB010000162 DATMBB010000000
VERSION     DATMBB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526502"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2713
                     /locus_tag="VNL92_03410"
     CDS             <1..>2713
                     /locus_tag="VNL92_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HWO93789.1"
                     /translation="RVDTSQVRLTAAPGGAGLIVAAPDDAIPVIDRLIETLDQSSVSN
                     RLAIRRYEVQHARADELARTLQTLLDAQRQGAGADVPPSRILPDARTNTILVTASDAQ
                     HRDVSRLIAEADIPMRRPDEEMAIIALRQAAPTTVRRVIDEVLVAKEPARRERIHISA
                     EDTSSVLVVRAPKDDLDEIRAIVAQVDQAETGGLPVRSIKLERADAAAVASSLQQFFR
                     DRSSARAGARGASRVAVVGDRRSGTLVVSANDDDFAQVQELVRTLDSPTPARDMAFKI
                     VQLKHARVGEVGATIKTIIDEMRWETMWGPQREDRVEVRVELNPRTNSVVLVGRGEAV
                     EVAERVVQALDLPDEARAAIAVRSVKVRAADLNAMRNVLQRAFATPGWQSWRGADPEA
                     LTVEVDRAQRALVLVGKRERVEQAAAYIAELDKGPEGASQKIEAITLQHAQADRVANS
                     LRQFFADRARMLGVDTPGVSVVGSRDGNVVIVSGDEDSIGQMRDLVAQIDQPDGGKDR
                     RVEVIVLKNAVANDVAGALRAMFARPGKDDERVIVTPQPSTNSLILSAPAAMYADVRA
                     LLDQLDAAPRAEETNIETIALSSVRAAEAAQALRTALPPTVKVTITPVTRSNSLMLTG
                     SKEAIAIVMEQVKKLDTEPIRSGLVFRRFRLEHAEAADVSYTVEQLLEARPASPTEPS
                     ASIDYNRADNTVTAYAPADQIEEIERIIRELDEAPAEDRLTEFVKLQYANASQTASAL
                     KVFYGRTASEAASAAARNVTILPDELSNSLVIRADKSQWEGIRALLAKLDTKDYDTTR
                     QLAVIPLIYADAVSVARALNEGLRAPLEEQLRQAQIREARARQGQPQRPTDRPEATVL
                     IDAQSIPTVSAEPQTNSLVVFAGGRDLERIRDIVRQLDVAGFAEMPTP"
BASE COUNT          529 a          967 c          793 g          424 t
ORIGIN      
        1 cgggtggaca cctcgcaggt ccgtctgacc gccgcaccgg gaggagcagg gcttatcgtc
       61 gcggcgccgg atgatgcaat tcccgtcatc gaccgactca tcgaaaccct cgaccagagt
      121 tccgtcagca accggcttgc catccgccgt tacgaagttc aacacgctcg ggccgacgaa
      181 ctcgcccgca cactccagac gctccttgac gctcaacgac agggcgccgg cgcggacgtt
      241 cctccctcgc ggatcctccc cgacgcgcgt accaacacga tcctcgtcac cgcaagcgat
      301 gcacagcacc gcgacgtatc ccgtctcatt gcggaagccg atattcccat gcgccgcccg
      361 gatgaagaaa tggcgatcat cgcgctccgg caggcggcgc caaccactgt tcgtcgcgtc
      421 atcgacgaag tcctcgttgc caaggaaccc gcgcgacgcg agcgaattca catttcggcc
      481 gaggacacgt ccagtgttct cgtcgttcgt gcgccaaagg atgatctcga cgagattcgc
      541 gccatcgttg cacaggttga tcaggcggaa accggcggcc tgccggtgcg ctccatcaaa
      601 ctcgaacgtg ccgacgctgc cgccgtcgct tcgagccttc agcagttctt ccgagaccgc
      661 tcatccgcac gcgctggggc acgcggagca agtcgcgtgg ccgtcgtcgg agatcgtcgc
      721 tccggcacgc tcgtggtttc cgccaatgat gatgacttcg cacaggtcca ggaactcgtt
      781 cgcactctcg acagcccgac ccctgctcgc gacatggcct tcaagatcgt gcaactcaag
      841 cacgcccgcg tgggagaggt gggcgccacg atcaagacga tcatcgacga aatgcgatgg
      901 gaaaccatgt ggggtcctca gcgcgaggac cgcgtcgagg tccgcgtcga actcaatcct
      961 cgcacgaaca gcgtcgtgct ggtgggaaga ggcgaggccg tggaggtcgc ggagcgcgtt
     1021 gtccaggcgc tggacctgcc cgacgaggcc cgcgctgcga tcgccgtcag gagcgtcaag
     1081 gtccgcgccg cggacctcaa tgccatgcgt aacgtcctgc agcgagcatt tgctacgccg
     1141 gggtggcaat cctggcgagg cgccgacccg gaggcgctga ccgtcgaagt tgaccgcgcg
     1201 caacgagcgc tcgttctcgt cggcaagcga gaacgggtcg agcaggccgc agcctacatc
     1261 gccgaactcg acaaagggcc cgagggcgca tctcagaaga tcgaagcgat cacgctccaa
     1321 cacgcgcaag ccgaccgcgt ggcgaactcg cttcgccagt tcttcgcgga ccgtgcccgc
     1381 atgctcggcg ttgatacgcc gggcgtcagc gtcgtcggct cgcgagacgg caacgtcgtc
     1441 atcgtctccg gtgacgagga cagcatcggg cagatgcgag acctcgtcgc ccagatcgac
     1501 cagccggatg gcggaaagga ccgccgtgtg gaagtgatcg tcctcaagaa cgccgtggcc
     1561 aacgacgtcg ccggcgccct gcgcgccatg ttcgcccgtc cgggcaagga tgacgagcgc
     1621 gtcatcgtta ctccccaacc atcgacgaac tcgctcattc tctccgctcc ggccgccatg
     1681 tatgccgacg ttcgtgcgct gctcgaccag ctcgatgccg ctcctcgtgc cgaggaaacg
     1741 aatatcgaga ccatcgcact cagcagcgtt cgtgcagccg aggcggccca ggctctccgc
     1801 acggcgcttc caccgaccgt caaggtgacc atcacgcccg tcactcgcag caactctctc
     1861 atgctcaccg gctccaagga ggcaatcgca attgtcatgg agcaggtgaa gaaactcgac
     1921 accgaaccta ttcgcagcgg cctggtattc cgtcgcttcc gtctcgagca tgcagaagcc
     1981 gcggacgtct cctataccgt cgagcagttg ctcgaggccc gacccgcaag cccgacggaa
     2041 ccttccgcaa gcatcgacta caaccgcgca gacaacaccg tcaccgccta cgctccggcc
     2101 gatcagatcg aagaaatcga gcgcatcatc cgcgaactgg acgaagcccc ggccgaggac
     2161 cggctcaccg agttcgtcaa actccagtac gccaacgcct cccagacggc cagcgccctc
     2221 aaggtcttct acggacgcac cgcgtccgaa gccgcctccg ccgcggcccg caacgtgacc
     2281 atccttcccg atgaactctc caactcactg gtgatccggg ccgacaagtc gcagtgggag
     2341 ggcatccgcg ctctgttggc caagctcgac accaaggact acgacacgac acgccaactg
     2401 gccgtcattc cgctcatcta cgccgacgcc gtcagtgtgg cccgcgcgct caacgaaggc
     2461 cttcgggcac cgctcgaaga acaactgcgg caagcccaga tccgcgaggc tcgcgcccgt
     2521 caagggcaac cacagcgtcc aaccgatcgc cccgaggcca cggtcctgat cgacgcccag
     2581 agtattccga ctgtttccgc ggaaccacag accaactccc tcgtggtctt cgcgggagga
     2641 cgcgatctcg agcgcatccg cgacatcgtg cgccaactcg atgtcgcggg cttcgccgag
     2701 atgccgacgc ccc
//
LOCUS       DATMBB010000163         2034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_60846, whole genome shotgun sequence.
ACCESSION   DATMBB010000163 DATMBB010000000
VERSION     DATMBB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60846"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..709
                     /locus_tag="VNL92_03415"
     CDS             17..709
                     /locus_tag="VNL92_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93790.1"
                     /translation="MRDEDLRALALRVGSELDERESRNTLQSWQLEDLQRALLFLTTF
                     AYVLLEQHASRDATSHIQQARRRLGVEGLRNLRGVVFLANMGYVAPARRLMAALADAV
                     LGLRYFRQFPDDAEAWVGGGGPDAELMRERLGAAGEPVESEGSAVPASRAAPPLLPLA
                     ALLGEGNQQEQVARTLQRVGRLGSDLVLLLAEGLEGAFDSVGTMPLSRSDLDDALSLV
                     EALLLSSSVRAR"
     gene            824..2026
                     /locus_tag="VNL92_03420"
     CDS             824..2026
                     /locus_tag="VNL92_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93791.1"
                     /translation="MEFRDNAEEASFRGEVRRFIEANMPKGGLSPEFAERTQFGEGNG
                     SPEEEAFLERWKEALRERGWVAPHWPKEYGGAGMSVSEQFVFNEEMAEARAPAVGGMG
                     VQMIGPILIMYGSEEQKKQHLPGITSGKVWWCQGYSEPGSGSDLASLQTRAVRDGDDY
                     VINGQKIWTSGAHRADWMFMLARTDPDAPKHRGISMFLLDMKSPGISVRPLVNMANDH
                     GFNEVFFDSVRVPARNMVGEENRAWYIGATLLDFERSNIRAAIQLRHTIDDLVRYVKD
                     GEAKTGQVNVTPSIRAELADRLVEAEVARLICYRIISMQKRGAVPNYEASVNKLFYSE
                     ASQRAAQTGMHIIGLYGALHRDPKWAPLRGRLAHAYMTSVSGTIAAGTSEIQRNVIAT
                     RGLGLPRS"
BASE COUNT          348 a          625 c          712 g          349 t
ORIGIN      
        1 gccgtaggcg tcgacagtgc gagacgagga cctccgtgcc ctagcgcttc gtgttggctc
       61 cgagctcgac gagcgggagt cgcggaacac actgcaatcc tggcaattgg aggacttgca
      121 gcgggcgctc ttgtttctca cgacgttcgc ctacgttctc ctggagcagc acgcatcgcg
      181 cgatgcgacg agccacatcc agcaggcgcg ccgtcgcctc ggtgtggagg ggctgcgcaa
      241 tctgcgcggg gtggtctttc ttgccaacat gggctacgtt gcgccggccc gtaggctgat
      301 ggccgctttg gcggatgccg tgctcggcct tcggtacttc cggcagttcc ccgacgatgc
      361 ggaggcttgg gttggagggg gcgggcccga tgcggagctc atgcgcgaac gcctcggcgc
      421 cgccggcgag ccggtcgagt cggaaggctc cgcggtaccg gcgagccggg cagcgccacc
      481 ccttctgccc ctggccgcgc tgcttggcga ggggaaccag caggagcagg tggcgcgtac
      541 gttacagagg gtcggccggc taggcagcga tctcgtgctc ctcctcgcgg agggacttga
      601 aggcgccttc gactcggtgg gtaccatgcc tctctcgcgc agcgatctcg acgatgcgtt
      661 gtctctcgtc gaggcgctcc tgctctcgtc ttcggtccgg gcgaggtagc gacaccaagc
      721 cacgccggcc cgcgcttctg ttgacaaggc ccttgcgggc gagcacagtg tcaggcggat
      781 cgtcgcgctg ctcggaagcg gccgcgcgga gaggaggaag gtgatggagt ttcgtgataa
      841 cgcggaggaa gcgtcgttcc ggggtgaagt ccgacgcttc atcgaagcga acatgccgaa
      901 aggtgggctg agtcctgagt tcgccgaacg cacgcagttc ggtgagggga acggctcgcc
      961 ggaggaggag gccttcctcg agcgctggaa ggaggcgctg cgcgagcgcg gatgggtcgc
     1021 gccccattgg ccgaaggaat acgggggcgc gggaatgagc gtctcggagc agtttgtctt
     1081 caatgaggag atggcggagg cgcgcgcccc ggccgtcggc ggcatgggcg tgcagatgat
     1141 cggtccgatc ctcatcatgt acggctccga ggagcagaag aagcagcacc tgccgggcat
     1201 caccagcggg aaggtgtggt ggtgccaggg atactcggag cccggttcgg gctccgatct
     1261 tgcgtcgctg cagacacgcg ccgtccgcga tggcgacgac tacgtcatca atggacagaa
     1321 gatatggacg tccggggccc atcgcgccga ctggatgttc atgctggcgc ggacggaccc
     1381 ggacgcgccc aagcatcgcg gcatcagcat gttccttctc gatatgaaga gccccggtat
     1441 cagcgttcgg ccgctcgtca acatggcgaa cgaccacggg ttcaacgagg tcttcttcga
     1501 tagcgtccgc gttcccgctc gcaacatggt gggcgaggag aaccgcgcct ggtacatcgg
     1561 cgccaccctc ctcgatttcg agcgctccaa cattcgagcc gccatccagt tgcggcacac
     1621 gatagatgac ctcgtgcggt acgtgaagga tggcgaggcg aagacgggcc aagtgaacgt
     1681 aacgccgtcc atccgtgccg agctggccga tcgtctcgtg gaggcggagg tcgcgcgcct
     1741 catctgctat cgcatcatca gcatgcagaa gcgcggtgca gtgccgaact acgaggcctc
     1801 ggtgaacaag ctcttctact cggaagcgag ccagcgtgcc gcccaaacgg gcatgcacat
     1861 catcgggctc tacggcgccc tccaccgcga tccgaagtgg gccccgctcc gaggccgctt
     1921 ggcgcatgcg tacatgacga gcgtttccgg aaccatcgcc gcgggaacga gcgagattca
     1981 acgtaacgtc atcgccacgc gggggcttgg cctaccgcga agctaagggt cgtt
//
LOCUS       DATMBB010000164         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_150064, whole genome shotgun sequence.
ACCESSION   DATMBB010000164 DATMBB010000000
VERSION     DATMBB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150064"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VNL92_03425"
     CDS             <1..540
                     /locus_tag="VNL92_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93792.1"
                     /translation="EAFSLGSRVVVLDEGRVLQEGGRDDIFERPASRRVAELVGVRNL
                     LPARVLERGEGGAIVAWRGRPLLVQRDDLSSGTMVDLAIRSSRVLIVRADRPVPERNS
                     FYGRIIEERLRAENYTLRFRIDGSDEPWDLEIDVPSYVYGRLGLAERKEVAVTLKAED
                     LHVIPQRRGDSAEPELTGP"
     gene            637..1776
                     /locus_tag="VNL92_03430"
     CDS             637..1776
                     /locus_tag="VNL92_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWO93793.1"
                     /translation="MNSSYHLGRVFGVPVGLNPSWFIAFGLIMFVLAQRVYPMAIPAR
                     GADIYWAMAFISGVLFFGSILAHELAHSVVAKAYGIPVNGITLFILGGVSQIAREAPR
                     ARQEFLMAFAGPAVSFVLAGLALAAWIASGPSNEPLTVVLEWLWLMNFGVGLFNLVPG
                     FPLDGGRVLRAALWGMSGSYSGATRWSAYVGRGIAWLMMAYAGASLVAGGDAAWATDV
                     LGAIWLFLIAVFLEVAARSTWVRTRTLLSLRQWTVGDVVEPRWPMVASTARLGAMEEE
                     GLVAEEDDHVLVCENGSVIGVIPGPVALAALDEGRDPTALELMLAASSLPALALHYSA
                     YDAWERMDGANVDFLPVVDGSAFAGLVRRDGLVRLIRPRPVPSET"
     gene            1773..>2621
                     /locus_tag="VNL92_03435"
     CDS             1773..>2621
                     /locus_tag="VNL92_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWO93794.1"
                     /translation="MTRLAWVPSTNRAGPGPRTALAVLAGKGTGLASRLLGRGGGTAL
                     PGLVAERLDPRIVERLASQVEAPRVVVTGTNGKTTTSRLLGAILRAAGHRPIRNGAGS
                     NLMRGIAATLLHEAGTGGRLPPDRPAVFEVDEATLVQAGRALRPNVLVFTNLFRDQLD
                     RYGEIDSVADVWREALQALLGDARIVLNADDPLVARLSSVPEGRSVLYGIEDRSVALG
                     EAEHASDSRWCPRCDRDYTYEALFFGHIGWWRCSQCGDHRPTPDVGAADVRLVDDERA
                     SFSLRVG"
BASE COUNT          361 a          806 c          932 g          522 t
ORIGIN      
        1 gaggccttca gtctcgggtc tcgcgtcgtt gtgctcgatg agggacgggt gcttcaggag
       61 ggcgggcgag acgacatctt cgagcggccg gccagccggc gcgtggcgga gctggtgggg
      121 gtgcggaacc tgcttcccgc ccgtgtgttg gagcgaggcg agggcggcgc tatcgtcgca
      181 tggcgggggc gaccgctcct cgttcagcgg gacgatctgt catcgggcac gatggtcgac
      241 ttggccatcc ggtcgtcgcg ggtcctcatc gtgcgtgccg atcggccggt tcccgagcgg
      301 aacagcttct atgggcgcat catcgaggag cgtcttcgtg ccgagaacta cacgctgcgc
      361 tttcgcatcg acggctcgga cgaaccgtgg gacctcgaga tcgacgtgcc ctcgtacgtg
      421 tacggaaggc tcgggctggc ggagcggaag gaagtcgcgg tgaccctcaa ggcggaggac
      481 ctgcacgtca tcccccagcg gcgcggcgat agcgcggagc cggagctgac gggcccgtag
      541 ggtcaaggat cgcttgcgtc cctattgggc gtacactaca gtggatgcgt tctcaattga
      601 gcgctgggtt taggcttagg ttgtcaggaa ctcgtgatga atagctcgta tcacctcgga
      661 cgggtgttcg gtgttcccgt tgggctgaat ccttcgtggt tcatcgcgtt cggtctcatc
      721 atgttcgtac tggcccagcg ggtctacccg atggcgattc ccgcccgggg cgccgacatc
      781 tattgggcga tggcgttcat ctccggggtg ttgttcttcg gctccatcct ggcgcacgag
      841 ctcgcgcaca gcgtcgttgc gaaagcctac gggatcccgg tcaacggaat cacgctgttc
      901 atcctgggcg gcgtctccca gatcgccagg gaggcgcccc gtgctcgtca ggaattcctg
      961 atggcgttcg ccgggcctgc cgtgagcttc gtcctcgccg gcttggcgct ggctgcttgg
     1021 atcgcgtccg ggccgagcaa cgagccgctg accgtggttc tggagtggct ctggctcatg
     1081 aacttcggtg tcggtctgtt caatctcgtg ccgggctttc ctctcgatgg cggccgcgtc
     1141 ctgagggcgg cgctgtgggg aatgtcggga agctattccg gtgcgacgcg ttggtccgcg
     1201 tatgtggggc ggggcattgc ttggctgatg atggcgtatg cgggtgcctc gctcgtcgcg
     1261 ggcggcgatg cggcctgggc caccgacgtt ctgggggcca tctggctctt cctgatcgcg
     1321 gtgtttctcg aggtggctgc tcgcagcacg tgggtccgaa cacgcacgct cctctcgctt
     1381 cggcagtgga ccgttggcga cgtcgtcgag ccgcgatggc cgatggtcgc ttcgactgct
     1441 cgcttgggcg cgatggagga ggaaggactc gttgccgagg aggacgatca cgttctcgta
     1501 tgcgagaacg gtagcgtcat cggtgtgata cccggcccag ttgccttggc ggcgctggac
     1561 gaggggcgcg accccacggc gttggagctt atgctcgctg catcgagcct tccggcgctt
     1621 gcgcttcatt actcggccta cgatgcatgg gagcgcatgg acggcgcgaa cgtggatttc
     1681 ctgcctgttg tcgacggttc ggcgttcgct gggctcgtcc ggcgcgacgg tctcgtgcgc
     1741 ctcattcgac caaggccagt ccctagcgag acatgacacg tttggcgtgg gtgccctcca
     1801 cgaaccgtgc tgggccgggc cctcggaccg cgctcgccgt gctcgcgggc aaagggacgg
     1861 gcctcgcgag caggcttctg gggcgaggtg gtggcacggc gctacccggt ctcgtcgcag
     1921 aacgactcga tccacggatc gtcgagcggc tcgcgagcca ggtggaagcg ccccgcgtcg
     1981 tcgtcaccgg gaccaatggt aagaccacca cgtcgcgtct gcttggcgct atccttcgcg
     2041 ccgccggcca tcgcccgatc cgaaatgggg cgggatcgaa cctcatgcga ggcatcgccg
     2101 ccaccctgct ccacgaagcg gggaccggcg gccgtctccc tcccgaccgc ccggccgtgt
     2161 tcgaagtgga cgaagcaacc ctcgtgcaag cgggacgggc gttgcgcccc aacgtgctcg
     2221 tcttcacgaa cctgtttcgc gaccagctgg accgctacgg cgagatcgat tccgtagcag
     2281 acgtctggag ggaggcgctc caggcgcttc tcggcgacgc tcgcatcgtg ttgaacgccg
     2341 acgatccctt ggtggctcga ctctccagcg ttccggaggg tcgaagtgtg ctctatggta
     2401 tcgaggacag atccgtcgcc ctcggcgagg cggagcacgc gagcgattct cgctggtgtc
     2461 cgcggtgcga ccgcgactac acctacgagg ccttgttctt cggacacatt ggctggtggc
     2521 gctgctcgca gtgcggcgac catcgcccga ccccggacgt cggggccgcc gatgtgcggc
     2581 tggtggatga cgaacgagcg tcgttctcgc tgagggtggg g
//
LOCUS       DATMBB010000165         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_331390, whole genome shotgun sequence.
ACCESSION   DATMBB010000165 DATMBB010000000
VERSION     DATMBB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331390"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VNL92_03440"
     CDS             complement(<1..284)
                     /locus_tag="VNL92_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93795.1"
                     /translation="MVNTFDVALKSEGDGFETPCLLLDFDRLRANASLVRTTFTPLQP
                     SIFYSVKANSHPSVLRVLSEEGLGFDVASIGEIMALVELGVPADRMIFSST"
     gene            complement(462..>2400)
                     /locus_tag="VNL92_03445"
     CDS             complement(462..>2400)
                     /locus_tag="VNL92_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWO93796.1"
                     /translation="VFAAADLGFTYLTLQEVYLSGNPIDLGWPLGLLLIGYATVRRWR
                     YGPLAVPASDEASRERAALMDKVLSFGPYLLVGLAMTLLFVAGFGERESERNVFIGLA
                     LSVIVLVLLRQFVTLVENQRLAGTLRSFNEILEATVEERTAKLANLQSTASALATAAS
                     AEEVCQVGLGAIRTAAPAQSAALYIRKYDAVERVAGDDVPGVSRLVAMLDGNSRATVQ
                     ASVDGAEGVHVALLPFRTGGQTEGVVVARVETSSLDLGHLAALASELGVAYENQIQLQ
                     EAKRLAERDPVTEVFNHRYLHTRLNALLDEAQAQGNPLSLVLADIDDFKLFNDTYGHQ
                     TGDEVLRMVARGLKELAPADAVIARFGGDEFAVILADAGESDVIAFCEGVQEWMGQRA
                     FEQPGSERIPVALTFGYATFPEHGKRRYELVAAADSRLYEAKRSGTPLPSKQPAARLE
                     TPGSFMFLDALITSVDNKDRYTKRHCDLVAEYAVMLAEEMKLSQGVQRSLSIAGALHD
                     VGKICIPDRILRKPGPLDDEEYDIIKLHVPLAANLIQHVPQRREVLDGVLNHHERWDG
                     KGYPKGTSGTNIPLVGRIMAVADAFSAMTLDRPYRQALPFEVAIGELRKHRGTQFDGD
                     LVDIFVTAFERRIGNHRHSAA"
BASE COUNT          427 a          742 c          804 g          427 t
ORIGIN      
        1 gtggagctga agatcatgcg atccgcgggg acaccaagct cgacgagcgc catgatctcg
       61 ccaatcgaag cgacatcgaa ccccagccct tcctcactaa ggacgcgaag caccgaggga
      121 tgcgagttcg ccttgaccga atagaagatc gacggttgga gtggtgtgaa ggtcgttcgt
      181 actagggagg cgttcgcccg caggcgatcg aaatcgagaa gaaggcaagg tgtctcgaag
      241 ccgtcgccct ccgacttgag ggcaacatcg aatgtattca ccatgggtct tgtgcgtccg
      301 ccctttcccc cgccatcgct tatcataacg gcgaaacggg cttatggcga gcgcaagtgc
      361 tcagaggcac atctatcctg cggccgccct tcccaacgac actaatcata acgcctgaag
      421 gccgcgcgcg gtgcggtggg gcgtgtcgcg taacggggag gttaggcggc ggagtggcgg
      481 tgattgccga tccgtcgttc gaaggccgtg acgaagatgt cgacgaggtc accatcgaac
      541 tgcgtgccgc ggtgcttccg cagctcgccg atcgccacct cgaaaggcaa ggcttggcgg
      601 tagggccggt cgagggtcat ggccgagaag gcgtcggcga cagccatgat ccggcccacg
      661 agcggaatgt tcgttccgct ggttcccttg gggtagcctt tcccatccca gcgctcgtgg
      721 tgattaagaa cgccatcgag cacctcgcga cgctgcggca cgtgctggat caggttcgcg
      781 gccaacggca cgtggagctt gatgatgtcg tactcctcgt cgtcgagcgg gccgggcttg
      841 cgaaggatcc ggtcggggat gcagatcttg cccacatcgt ggagtgcccc ggctatcgag
      901 agtgagcgct ggacgccctg ggacagcttc atctcctccg ccagcatgac ggcatactcg
      961 gccaccaggt cgcagtggcg cttcgtgtag cggtccttgt tgtcgacgga agtgatgagc
     1021 gcatcgagga acatgaatga gccaggcgtt tcgagccttg ccgcgggctg cttcgacggc
     1081 agcggggtac ccgagcgctt ggcctcgtac agtcgcgagt cggcggcggc cacgagctcg
     1141 tagcggcgct tcccgtgctc ggggaaggtc gcgtagccga aggtgagggc gaccgggatg
     1201 cgctcgctcc ccggttgctc gaaggcccgt tggcccatcc actcttgcac gccctcgcag
     1261 aacgcgatta cgtcggactc tcccgcatcg gccagaatca cggcgaattc gtcgccgccg
     1321 aagcgcgcga tgacggcgtc ggccggcgca agctccttca gcccgcgcgc caccatgcgc
     1381 agcacttcgt cgccggtctg gtggccatac gtatcgttga acagcttgaa gtcgtcgatg
     1441 tcggcgagga cgagcgaaag tgggtttcct tgcgcctgtg cctcgtcgag cagcgcgttc
     1501 aatcgcgtgt ggaggtaccg gtggttgaac acctcggtga ccggatcgcg ctcggcgagc
     1561 cgcttcgctt cctggagctg gatctggttt tcgtatgcga cgccgagctc gctggcgagc
     1621 gccgcgaggt ggccgaggtc gaggcttgag gtttcgaccc ttgccacgac gacgccctcc
     1681 gtttggccgc cagtccggaa cgggagaaga gcgacatgaa cgccctctgc gccatccacg
     1741 ctggcttgca ccgtggctcg gctatttccg tctagcatgg cgacgaggcg cgagaccccg
     1801 ggcacatcgt ctccggcgac ccgctccacc gcgtcgtact tgcggatata gagcgcggcc
     1861 gattgggcag gggccgccgt tcggatagct ccgagaccga cctggcaaac ctcctcggcc
     1921 gacgcggctg tcgcgagggc ggaggcggtc gattgcagat tcgcgagctt cgccgtgcgc
     1981 tcttcgaccg tggcttcgag tatctcgttg aaggagcgga gtgtgccggc gagacgctga
     2041 ttctctacga gcgtgacgaa ctggcgtagg aggacgagca cgatcacgga gagcgccagg
     2101 ccgatgaaga cattgcgctc ggattctcgc tcaccaaagc cggcgacaaa cagaagcgtc
     2161 attgcgagcc caacgagcag gtacgggccg aagctcagca ccttgtccat gagcgctgcc
     2221 cgctcccgcg atgcttcgtc gcttgcgggt acggccagcg gaccgtaccg ccaccgccgc
     2281 acggtcgcat aaccgatgag cagcaggccc agcggccagc cgaggtcgat ggggttgccg
     2341 ctcagataga cctcctgaag ggtcaggtag gtgaacccga ggtcggccgc agcgaatacc
//
LOCUS       DATMBB010000166         3851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_2195, whole genome shotgun sequence.
ACCESSION   DATMBB010000166 DATMBB010000000
VERSION     DATMBB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2195"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VNL92_03450"
     CDS             complement(<1..1416)
                     /locus_tag="VNL92_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HWO93797.1"
                     /translation="MEASIPIKFGTDGWRAIIAEDYTFTNVRACMEATCRFLEGEGLA
                     GRGLVIGWDTRFGSRAFAEACAEVAAAHGITVHLTEGPAATPIVSFALLRKRAGGAVV
                     ITSSHNPAMWNGFKYKPEYAGSASPEVIAGIESHLPAIFAGGEPPRRPLADAEAEGLV
                     RRFDPAPAYEQHVAQLVDLDGIRSASLRVAVDSMYGAGAGWFNRLLAGGGTKVRELHG
                     EVNPAFPGIAAPEPIAKNLALLLEGIASDGADVGLATDGDADRLGIVDERGAFVNQLQ
                     VFALLTYYLLEVRGARGPIVKSVTTTQMVNRLGELYGVPVHETPVGFKYLGPKMMETD
                     ALIGGEESGGYGFRGHIPERDGILAGLYFLDFIARTGRRPSELLADLYAKVGPHFYDR
                     IDVHLDPERRKAIEDRVIAAKPDELAGVRVVSRDTTDGYRFLLEGGGWLLIRFSGTEP
                     LMRVYTEVRDEALVPKLLEAGR"
     gene            complement(1416..2531)
                     /locus_tag="VNL92_03455"
     CDS             complement(1416..2531)
                     /locus_tag="VNL92_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HWO93798.1"
                     /translation="MREGAPLHYTRCMKAIVLVGGEGTRLRPLTYSRPKALVPVLGRP
                     FLLHFIEYLRGHGFDDVILAVAHGADAIRAALGDGRDAGVRLTYVDEPVPLGSAGAIA
                     NCAPNLGEETFLVANGDIVTDIDLSAMLRQHRERPALVTVALISVEDPSGFGVAEIDE
                     DERIRRFVEKPPPEEAPSSWANAGLWMFEPQAVSFIPPGRSMVETDLFPSLADAGLLG
                     GYRSGGYWIDLGTPRRYLQLHADLLARSALCSDAEALIEPGASIKQPVSIARGAVVRQ
                     GARIEGAAVVGIGSEVASGALVTASVLWERVIVERGAVVRNSIVAEETVIGAGAVVED
                     ATIGPGAAIEAGARTPHGLRLDPGERFPQAEAAQELT"
     gene            complement(2544..2939)
                     /gene="rpsI"
                     /locus_tag="VNL92_03460"
     CDS             complement(2544..2939)
                     /gene="rpsI"
                     /locus_tag="VNL92_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWO93799.1"
                     /translation="MSQQMYHYGLGRRKTAIAKIRLYPGSGTVVVNGKPMEEVFHRDV
                     HRRVIEQPLRVTENWGKYNVQAVVRGGGVTGWAGAMSHGIARALVVADDRHRATLRRH
                     GLLTRDAREKERKKYGLKRARKAPQYTKR"
     gene            complement(3383..3784)
                     /gene="rplM"
                     /locus_tag="VNL92_03465"
                     /pseudo
     CDS             complement(3383..3784)
                     /gene="rplM"
                     /locus_tag="VNL92_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122452.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
BASE COUNT          590 a         1342 c         1270 g          649 t
ORIGIN      
        1 ccgcccggcc tcgagcagtt tcggcacgag cgcttcgtcg cgcacctcgg tgtagacgcg
       61 catgagcggc tcggtaccgg agaatcggat gagcagccat ccaccgccct cgagcaagaa
      121 gcggtagcca tccgtcgtat ccctcgatac gacgcggaca ccggcgagct cgtcgggctt
      181 ggccgcaatc acgcgatcct cgatcgcctt cctcctctcc ggatcgaggt ggacatcgat
      241 ccggtcgtag aagtgcgggc caaccttcgc gtagaggtcc gcgagcagct ccgatggtcg
      301 ccggccggtg cgtgcgatga agtcgaggaa gtacagccct gcaagaatgc cgtcgcgctc
      361 gggaatgtgg ccgcgaaatc cgtacccgcc gctctcctcg ccgccaatga gggcgtcggt
      421 ctccatcatc ttggggccca ggtacttgaa gccaaccggc gtttcatgca ccggcacgcc
      481 atacagctcg cccaggcggt tcaccatctg cgtcgtcgtg acggacttga cgatcggtcc
      541 gcgggcgccg cgtacctcga ggaggtagta cgtgaggagg gcgaagacct gcagctggtt
      601 cacgaatgcg ccacgctcgt cgacgatgcc gagccggtca gcatcgccat cggtcgcgag
      661 gccaacgtcg gcgccgtcgc tcgcgatgcc ttcgagcagc agggcgaggt tcttcgcgat
      721 cggctcgggc gcggcgatgc cggggaaggc ggggttcacc tcgccatgca gctcacggac
      781 cttcgtgccg ccacccgcga ggaggcgatt gaaccagccg gcccccgccc cgtacatcga
      841 atcgacggcg acgcgcaggc tggccgaacg gatgccgtcg aggtcgacga gttgggcgac
      901 gtgctgctcg tatgccggag cagggtcgaa gcgccgcacg agcccctccg cctccgcgtc
      961 cgcgagcggt cgtcgcggcg gctcgccacc ggcgaagatc gccggtaggt gcgattcgat
     1021 gcccgcgata acctcgggcg aagcgctccc tgcgtattcc ggcttgtact tgaagccgtt
     1081 ccacatcgcg gggttgtggc tcgatgtgat gacgacggcg ccccctgccc gctttcgaag
     1141 caaagcgaag ctcacgatgg gcgtggccgc tggtccctcg gtaaggtgga cggtgatgcc
     1201 gtgcgccgcc gccacttcgg cgcaggcctc cgcgaaggca cgcgagccga accgcgtgtc
     1261 ccatccgatg acgagaccgc gaccggccag cccctccccc tccagaaagc gacacgtcgc
     1321 ttccatgcag gcccggacgt tcgtgaacgt gtagtcctcg gcgatgatgg cgcgccatcc
     1381 gtccgtgccg aacttgatcg ggatcgatgc ttccattacg tcagctcctg cgctgcttcg
     1441 gcctgcggaa agcgctcgcc cggatcgagt cgcaagccat ggggcgttcg ggcgcccgct
     1501 tcaatggccg cgccgggccc gatcgtcgcg tcctccacga cggcgcccgc cccgatgacc
     1561 gtctcctccg ccacaatcga attcctcacg accgcgccgc gttccacgat gacgcgctcc
     1621 caaagcacgc tcgcggtgac gagcgcgccg ctcgcgacct ccgagcctat gccaacgaca
     1681 gcggcccctt cgatgcgcgc cccctgacgc acaacggccc ccctcgcgat tgaaaccggc
     1741 tgcttgatgc ttgcgccggg ctcgatgagc gcttccgcat cggagcacaa agctgaccgc
     1801 gccaggaggt cggcgtggag ctgcaggtaa cgacgcggcg taccgaggtc gatccagtac
     1861 ccgcccgacc ggtagccgcc cagtagaccc gcgtcggcca ggctggggaa cagatcagtc
     1921 tccaccatgg aacggccggg cgggatgaat gacaccgcct gaggctcgaa catccacagc
     1981 ccggcgttcg cccaggagct cggcgcttcc tccggcggcg gcttctcgac gaaccggcgg
     2041 atgcgttcgt cctcgtcgat ctccgcgacg ccgaaccccg acggatcttc gacggagatg
     2101 agcgccaccg ttacgagcgc tggtcgctcg cgatgctggc gcagcatggc gctcagatcg
     2161 atgtccgtga cgatgtcccc gttcgcgacg aggaacgtct cctcgcccag gtttggggcg
     2221 cagttcgcga tggcgccggc ggagccgagg gggacgggct catcgacgta ggttaggcgg
     2281 actcctgcgt cacgcccgtc gccgagtgcg gcgcgtattg catcagcgcc atgcgctacg
     2341 gccaggatca cgtcgtcgaa gccgtgtccg cgaaggtact cgatgaagtg gagcaggaac
     2401 gggcggccga gcaccggaac gagtgccttg ggccgggagt acgtgagggg gcggagcctg
     2461 gtaccctcgc cccctaccag cacgatggcc ttcatgcagc gagtgtagtg cagcggcgca
     2521 ccctcgcgca cgccatgcct cggctaccgc ttggtgtact ggggcgcttt tctggcgcgc
     2581 ttgagcccgt acttctttcg ttccttctcg cgcgcgtcgc gcgtgagcag cccatgacgc
     2641 cggagcgtcg cccgatgccg atcgtctgcc acaacgaggg cgcgggcgat cccgtgggac
     2701 atcgcgcccg cccaacccgt cacgccacca ccgcgaacga ctgcctgcac attgtacttg
     2761 ccccagttct cggtcacgcg caatggctgc tcgatgacgc ggcggtgcac gtcgcgatgg
     2821 aagacctcct ccataggctt gccattgacg acgacggtgc ccgagccggg atagagccgt
     2881 atcttggcga tggccgtctt ccttcggccc aggccgtaat ggtacatctg ctgactcatg
     2941 cgtcgcttcc ttccgcatcg tcgctcttct tggcgcgagt ccgacgcgga cgcgccggcc
     3001 ggctcggttt cacgtcgtcc gaagcttcgg gctcgggcgc cggcgcgtcg gcgaggagat
     3061 cctccggctc ggcccccgcg accgccgagt ccatgacctc ctcgacgatg tcctcgggcc
     3121 tctgggtcgg cgcatcccca cgtgccggag catccggtgc gagcagctcc tccggctccg
     3181 acgccgctat cgcggagtct atggcttcct cgatggcctc ctccggctcg agtgcgggag
     3241 tggccttgcg ggcgcgggag gcggccgcct ttcgcctcga ctgggcaggc gccgccgccg
     3301 tcgctgctgc cgcgggcggc ggctcggctg cagtggccgc tttggcgcga cgggctgcgc
     3361 gagccccgtc cccggcccgc acctgggcct cgtggggatg ggttgggcct gcatagacct
     3421 tcaggtggtg catcaccttg cggccgagcc ggttgtgggg cagcatgccc ttcaccgcct
     3481 gctcgatgac ccgctccggg tgcgtcgcga tcatctggcg aaaggtgcgc tctttcaggc
     3541 cgcccgggta gccggagtgc cgaacgtatt tcttcgcgtc ggggtcgctc gaaaggcgca
     3601 ccttctcggc gttcacgacg acaacaaaat cgcccatgtc gagatggggc gagaacgttg
     3661 gcttgtgctt gccgcgcatc agcgaagcgg cccgtgccgc aagcctgcca agcgggacat
     3721 cggtcgcatc cagcacgtgc caggtacgtt ccacctccgc agccttcagc gtgtacgtct
     3781 tcatgtcaca tcctcgaaga gaggcggatc gtatcgaacc cgccagaggt agagtccttc
     3841 gggcggagca a
//
LOCUS       DATMBB010000167         2583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_30571, whole genome shotgun sequence.
ACCESSION   DATMBB010000167 DATMBB010000000
VERSION     DATMBB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30571"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..565
                     /locus_tag="VNL92_03470"
     CDS             98..565
                     /locus_tag="VNL92_03470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWO93800.1"
                     /translation="MLDVGSPAPDFSAPLDTGETFTLADWRGKKNVVLYFYPKDNTRG
                     CTQEACTFRDNYDEIGKYDAIIVGVSSDSVESHQGFREKHNLPFPLIADTDKRVSKLY
                     DARGLFGFMTARVTYVIDKDGVIRDAFRHEMAIGDHLRDVLAALEKMTSAAPS"
     gene            complement(591..1145)
                     /gene="rfbC"
                     /locus_tag="VNL92_03475"
     CDS             complement(591..1145)
                     /gene="rfbC"
                     /locus_tag="VNL92_03475"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWO93801.1"
                     /translation="MVKTKLPGVLIIDTDFFRDERGFFIESYHRQRYAEHGITDEFVQ
                     DNHSRSAKGVLRGLHYQDMTAPMGKLVRCTRGAIWDVAVDLRAGSPTFGQWVGVELTE
                     QNMRQLLVPVGFGHGFVALEDGSDVQYKCTGFYTPASEGTIAWNDADLGIEWPIADPI
                     VSKRDSQGRSLREYAAAPAFRYVA"
     gene            1220..2452
                     /locus_tag="VNL92_03480"
     CDS             1220..2452
                     /locus_tag="VNL92_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWO93802.1"
                     /translation="MEHPRIYLDHAATTAPDPRVVEAMMAAYTSTWGNPSSLYEEGQR
                     AHAALDAARRTCAAILGCRPNEIVFTSGGSESDNLALRGAAYGALSRGRHIISTVMEH
                     HAVLHPLERLESEGFRVTYLPVDREGLVDPADLERAVTDETTLVSIMHANNEIGSVQP
                     VAELSRVAKKRRPGVIFHTDAVQSAGYLDLNVEALGVDLLSISAHKLYGPKGSGLLYV
                     RNRTPFVPQILGGSQERNRRAGTENVPGIVGLVEALRLAAEEREARTTKARRLRDRLL
                     AEVPQRIPNTAITGPLDGDRRLPNNASFCFGYVEGESILLQLDLNAISASSGSACTTG
                     AVEPSHVLVGTGVPEPLCRSSLRLTVGKDTTDEDIDRVLDVLPRIVADLRAMSSGPVQ
                     DGAAIAEWVERRAALPTS"
BASE COUNT          460 a          868 c          793 g          462 t
ORIGIN      
        1 cccgcccctt ctttacgtgc tagcgttgtt gccgctcgct acatggagtg gccatggtat
       61 agtgtatagt acaggcaaat gtatcgcgga ggctgccatg ctcgacgttg gatcccctgc
      121 acctgacttc tcagcgccgc tcgacaccgg cgagacgttc acgctcgccg attggagagg
      181 gaagaagaac gtcgtcctct acttctatcc caaggacaac actcgcggct gcacgcaaga
      241 ggcctgcacg ttccgcgaca actacgacga aatcggcaag tacgacgcga tcatcgtggg
      301 cgtcagcagc gattccgtcg aaagtcacca ggggttccgg gagaagcaca atcttccctt
      361 cccactcatc gccgacacgg acaagcgcgt ctcgaagctt tacgatgcgc gtggcctgtt
      421 cgggttcatg accgctcgcg ttacgtacgt catcgacaag gatggggtca tccgcgacgc
      481 cttccggcac gagatggcca tcggcgatca cctgcgcgat gtcctcgcag ccctagagaa
      541 gatgacttcc gctgcgccca gttgagcggg cggggcttag ccgcgcgcac tcatgccaca
      601 tagcggaagg ccggcgcggc agcgtactca cgtaggctgc ggccctggct gtcgcgcttc
      661 gacacgatcg ggtcggcgat gggccattcg atgcccaggt ccgcgtcgtt ccacgcgatc
      721 gtgccttcgg atgcgggagt gtagaacccg gtgcacttgt actgcacgtc cgagccatcc
      781 tccaaagcga cgaacccatg cccgaatccg accgggacga gcagttggcg catgttctgt
      841 tcggtcaact ccacgccgac ccactgtccg aacgtcgggg agccggctcg caagtcgacc
      901 gcgacgtccc aaattgcgcc gcgtgtgcag cggacaagct ttcccatggg cgcggtcatg
      961 tcctgatagt ggagcccgcg cagcacgcct tttgccgaac ggctatggtt gtcctgcacg
     1021 aactcgtccg taattccgtg ctcggcgtac cgctgacggt gatagctctc gatgaagaag
     1081 cctcgctcgt cgcgaaagaa gtcggtatcg ataatgagaa cgccgggcag cttggttttc
     1141 accacgctga tctccatgtc gcctccgcca tgcttctcgc gtctttaccc gccctacccg
     1201 cggtgctagc ctgatgatga tggaacatcc tcgcatctac ctcgaccacg ctgcaacgac
     1261 tgcgccggat ccgcgagtcg tcgaggccat gatggccgcc tacacctcga cctgggggaa
     1321 cccctccagc ttgtacgagg aaggccagcg tgcccacgct gccttggatg ccgcgcgtcg
     1381 cacctgcgcc gccatcttgg gctgccgccc taacgaaatc gtgttcacga gcggcggcag
     1441 cgagagcgac aatctggccc ttcgaggcgc cgcttacggc gctctttccc ggggccgcca
     1501 catcatcagc accgtcatgg agcatcacgc tgtcctgcac ccgctggagc ggctggaatc
     1561 cgaagggttc cgcgtcacct acctccctgt cgatcgcgag ggccttgtcg acccggcgga
     1621 cctggagcgc gctgttaccg acgagacgac gctcgtctcg attatgcacg cgaacaacga
     1681 gatcggcagc gtgcagcccg tggccgagct cagtcgcgtc gccaagaaac gccggccggg
     1741 agtcatattc cacaccgacg ccgttcagtc ggccggatac ctcgacctga acgtcgaggc
     1801 gcttggcgtc gacctgctgt ccatttcggc gcacaagctc tacgggccca agggcagcgg
     1861 tctgctgtac gtgcgtaacc gaacgccgtt cgtgccgcag atcctcggcg ggtcgcagga
     1921 gcgcaaccgg cgcgcgggga ccgagaacgt gcccggaatc gtcggactcg tggaagcgct
     1981 ccgcctggcc gctgaggagc gcgaggcgcg gacgacgaag gcgcgccgtc tccgagatcg
     2041 gctgctcgcc gaagtgcccc agcgcatccc gaatacggcc atcacaggcc ctctggatgg
     2101 cgaccgccgc cttccgaaca acgcgagctt ctgcttcggc tacgtggagg gcgaatcgat
     2161 tcttctgcaa ctcgacctga acgcgatcag cgcctcgagc ggatcggcct gcacgaccgg
     2221 cgccgtcgag ccttcccatg tgctcgtggg caccggcgtt ccggagccgc tgtgcagaag
     2281 cagcctccgc cttacggtgg gcaaggacac cacggacgag gacatcgatc gcgtgctcga
     2341 cgtgctcccg cgcatcgtgg ccgacctgcg tgcgatgtcg tccgggccgg tgcaagatgg
     2401 cgctgccatc gcggagtggg tggagcgccg ggctgcgcta ccaaccagct gagccaaggc
     2461 gcgtcgaggt cgacagttcc cgtttgctcc agctatcatt ttgccgcgta gacgcgctct
     2521 tccgctcgcc gaacacgctc cgaggaggcc gaaacgatgc ccagcaaggt cgaccgtgac
     2581 ctg
//
LOCUS       DATMBB010000168         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_130035, whole genome shotgun sequence.
ACCESSION   DATMBB010000168 DATMBB010000000
VERSION     DATMBB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130035"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VNL92_03485"
     CDS             <1..374
                     /locus_tag="VNL92_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HWO93803.1"
                     /translation="LYLTQLHYEGSEALCRGVGSCAEVNDSEYATVGPLPVALLGFTA
                     YATMLAIAVAAHLRPAGAPVFALAAFGIALTGAGYSGYLTYVELFVIEAICPWCVVSA
                     VVMTALLATTIRPALAPPGAR"
     gene            complement(434..1228)
                     /locus_tag="VNL92_03490"
     CDS             complement(434..1228)
                     /locus_tag="VNL92_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO93804.1"
                     /translation="MSTQTEYYRSHWVTIEDERLARYDLMFRWGPHLDPLFDGFDVGE
                     GMSVADYGCGPGWVAIEMARRVGPSGRVYGCDLNQTFLSVAAQHGEEAGVADVVEWRH
                     VADDRVPLPDRSVDRVFCKNVLEYVPSIDSTLAEFRRVLKPGGLMRLVDSDWDMIVIE
                     PLGAQRTDELMRYARHAYNDAQAGRHLYGAARRVGFDEVQVRVTVNADTQGNIRVVLS
                     NILGYAVAGGLAEAEAQRMAAEIDAAIERREFMALLPQFIVTAVAP"
     gene            complement(1502..>2560)
                     /locus_tag="VNL92_03495"
     CDS             complement(1502..>2560)
                     /locus_tag="VNL92_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HWO93805.1"
                     /translation="EADLHKADLSAADLREANLVTADLSGANLIGGSLGGAALRGANL
                     MDANLIGENLRGADLIEANLIGANLIDANLSGADLHNAMFGTVLGRCSLRSVKGLETC
                     QHLFPSFLDFHTLASSGPLPLPFLRGCGLPDRLIEKLPSLLNEPSELDEPSEYHSCFI
                     SYSSKDEKFAQQLYADLQDKGVPCWFARKDLPIGGRQREIFDRAIQLHDRTLLILSKH
                     SVASDWVEKEVETTFEKERKQKRTALFPIRIDNAVMEAECGWAADIRRTRHIGDFRGW
                     RNPEKYQKALEVLLRDLRASDEAAFVMDDDQVRLVGKGSIVDRTAGMLKSRKPVASTQ
                     ALRETSEQGFAEEATGLQ"
BASE COUNT          409 a          870 c          826 g          455 t
ORIGIN      
        1 cgctctacct cacgcaactg cactacgagg gaagcgaggc attgtgccgt ggcgtgggca
       61 gttgcgccga ggtgaacgac agcgagtacg cgaccgtcgg gccgctgccc gtggcgctcc
      121 tcggcttcac ggcgtacgcg acgatgctcg cgatcgccgt cgccgcgcac cttcggccag
      181 cgggagcccc tgtattcgca ctggcagcat tcggcatagc gctcaccggc gcgggctata
      241 gcggctacct gacgtacgtg gagctcttcg tcatcgaggc catctgtccc tggtgcgtcg
      301 tctccgccgt cgtgatgacg gccctcctgg cgaccaccat ccgtccggca ctcgcgccgc
      361 ccggcgcgcg atagcagcgg gccctgtccg cgtccctcga acgcggacgg ggccacgcgg
      421 atcggtgtgg gatctacgga gcgaccgcgg tcacgatgaa ctgcggcagg agggccatga
      481 actccctccg ctcgatggcc gcgtcgatct cggccgccat tcgctgcgcc tccgcctcgg
      541 cgaggccgcc cgcgaccgcg tacccgagga tgttgctaag gacgacgcgg atgttgcctt
      601 gggtgtcggc gttgaccgtg acgcgcacct gcacctcgtc gaagccgacg cggcgcgccg
      661 cgccgtagag atgacgcccg gcctgcgcat cgttgtaggc gtggcgagcg tagcgcatga
      721 gctcgtccgt gcgctgcgct ccaagcggct cgatgacgat catgtcccaa tcggagtcga
      781 cgaggcgcat gaggccgccc ggcttcagca cgcgccggaa ctccgccagc gtgctgtcga
      841 tggacgggac gtactcgagc acgttcttgc agaagacgcg atcgaccgag cgatcgggca
      901 acggaacgcg gtcgtcggcg acatgacgcc attcgaccac gtccgcgacg ccggcttcct
      961 ccccatgctg cgcggcgacg gagaggaagg tttggttgag gtcgcagccg tagacgcggc
     1021 cgctgggacc gacccggcgc gccatctcga tggccaccca gccgggcccg caaccgtagt
     1081 cggccacgct catcccctcg ccgacgtcga agccatcgaa gagggggtcg aggtgcggcc
     1141 cccatcggaa catgaggtcg tagcgggcga gccgctcgtc ctcgatcgtc acccagtgcg
     1201 agcggtagta ctccgtttgc gtcgacacgc cagtcttcct ccctcgtccg tcttcggctc
     1261 ccttggcgct gcccccggcg cggccgttct gccggtcgcg ccgagaccca ggacgcgcaa
     1321 gacatagccc ccgcccgcac cccgtgtcaa ccctcccgcc tcgccgcgat ggaccaaggc
     1381 atactttgac ggcggagcgc attcgctgca gatagtccgt agcgcgtggc gactgctccg
     1441 gctcatcgtc gaggacgtgg cgcagcagga tgttggtgtc gaggaagggt agagtcagcg
     1501 cctactggag ccctgtcgcc tcctcggcga acccctgctc ggacgtctcg cgaagcgctt
     1561 gcgtgctggc gaccggcttg cgggacttca gcatcccagc ggttcggtcc acaatgcttc
     1621 ccttccccac caaccggacc tgatcgtcgt ccatcacgaa ggccgcttcg tcggaagcgc
     1681 gaagatcgcg taggagcacc tcaagcgcct tctggtattt ctccgggttc cgccaaccgc
     1741 ggaagtcgcc gatatggcgc gtgcggcgga tgtcggcggc ccagccgcat tctgcctcca
     1801 tgaccgcatt atcgatccgg atgggaaaga gcgccgtccg cttctgcttc cgctcctttt
     1861 cgaacgtcgt ctcgacctcc ttctcgaccc agtcacttgc aacggagtgc tttgaaagta
     1921 tcagcagggt gcgatcgtga agttgaatcg cccgatcgaa tatctcacgc tgcctgccgc
     1981 cgatcggcag gtccttacgt gcgaaccagc aaggcacacc cttgtcctgg aggtcggcat
     2041 agagctgctg cgcaaatttc tcgtccttgg atgagtagct gataaagcaa gagtggtact
     2101 cggatggttc gtcgagctcg gatggttcgt tgagcaatga aggcagtttt tcgatgagcc
     2161 ggtccggcag cccacacccg cgcaggaatg gcaacggcag cgggccggag gacgccagcg
     2221 tgtggaagtc gaggaagctc gggaagagat gctggcacgt ttcgaggccc ttgaccgatc
     2281 gcaagctgca tcgccctagg accgtgccga acatggcgtt atgcaggtcc gccccgctga
     2341 ggttcgcatc gatgaggttc gccccgataa ggttcgcctc gataaggtcc gctccgcgga
     2401 ggttctctcc gatgaggttc gcatccatca gattcgcccc gcggagggcc gctccaccga
     2461 ggctcccccc gatgaggttc gccccgctga ggtccgccgt cacgaggttc gcctcgcgga
     2521 ggtccgccgc actgaggtcc gccttgtgga ggtccgcctc
//
LOCUS       DATMBB010000169         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_371925, whole genome shotgun sequence.
ACCESSION   DATMBB010000169 DATMBB010000000
VERSION     DATMBB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371925"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VNL92_03500"
     CDS             <1..648
                     /locus_tag="VNL92_03500"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93806.1"
                     /translation="VAVIGATGCGKSTLLRIAGGLLTPSSGTVLIDERPPQDAQRHKR
                     IGFVFQDPALLPWRSVRSNVRLPLEVNRRQASTPADVDTLLATVGLAGAADRRPHELS
                     GGMRRRVALARALALDPALLLMDEPFAALDELTRESMRYELLRIWERHRATVLFVTHS
                     VREAVLLADRVVVMTPGPGRIRAIVPVDLPRPRRTTLERDPAFLDRVDRLRDLLS"
     gene            645..1496
                     /locus_tag="VNL92_03505"
     CDS             645..1496
                     /locus_tag="VNL92_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO93807.1"
                     /translation="MTTETGSPRTGMLGSIRSWTGESRQALAEVLRWLVSVALPPVLL
                     AAAAVALWELWVRWTGTAEWLVPAPTSVGERLIEDPRFFAREGLVTLTEALLGLGVGS
                     AAALALAIVMTHSRFLERGLYPLAIGLKVTPVVVWAPLFALWFGFGTTPKVFVAALIT
                     FFPMLVNTVVGLRDVDPDAHAFLRSTGASRVEVLWFLRLPYALPYVFAGLRISALLAV
                     IGAVVAEWSGADDGIGHAIAVANNNLDTPTVFAGIITLAVIGAILNAILALAERLVID
                     WHATQRA"
     gene            1522..>2241
                     /locus_tag="VNL92_03510"
     CDS             1522..>2241
                     /locus_tag="VNL92_03510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93808.1"
                     /translation="MCTIAVAVIALACGDDDDATAGTATAGDAAPERAAMTFLAGFRP
                     QANLPFVGAYVAQENGYFEEEGLDVTIEHSEGGPSNLQLVAQGRVQVTTADAAILLQR
                     RADPGLPIVAIALIGQRGQQGWATLADAGLDSPADWAGKRVGYRNVIPPDLPAILDAN
                     GLSLADVEATDIGFQPPQLLVEGIVDVYPVFLSNEPDTIRRVLGREVRVVSAADYGVP
                     TLGLTYVASEEYAQEHADELSR"
BASE COUNT          312 a          821 c          719 g          389 t
ORIGIN      
        1 gtcgccgtca tcggcgccac tggttgcggc aagtcgaccc tcctgcgaat cgcgggcggc
       61 ctcctgacgc cctcatcggg caccgtcctc atcgatgaga gaccgccaca ggacgcgcaa
      121 cgccacaagc gcattggatt cgtgttccag gacccggccc tgctgccttg gcggagcgtg
      181 cgctccaacg tccggctacc cctcgaggtg aaccgcaggc aagcgtctac gcccgccgac
      241 gtcgacaccc tgctcgcgac cgttggcctc gccggcgcgg ccgaccgccg cccgcacgag
      301 ctctccggag gcatgcgccg gcgcgtcgcc ctggcccggg cgctcgctct cgaccccgcg
      361 cttctcctga tggacgagcc gttcgccgcg ctcgacgagc ttacgcggga gtcgatgcgc
      421 tacgagcttc tgcgcatctg ggaacgacat cgcgcgacgg tgctgttcgt cacgcacagc
      481 gtgcgcgagg ccgtactcct cgccgaccgc gttgtcgtga tgactccagg gcctggccgc
      541 atccgcgcga tcgttcccgt cgacctgcct cgtcctcgcc ggacgacatt ggaacgtgat
      601 ccagccttcc tcgaccgggt cgatcggctg cgcgacctcc tctcatgacg acggagaccg
      661 gttcgccccg aacaggaatg ctcgggagca ttcgctcctg gacgggcgaa tcacggcaag
      721 ccctggcgga ggtccttcgc tggctcgtgt cggtcgccct cccgccggtg ctcctcgctg
      781 cggcggccgt cgccttgtgg gagctgtggg tgcgctggac cggcaccgcg gagtggctcg
      841 tgcccgcccc caccagtgtc ggcgagcgat tgatcgagga tccgcggttc ttcgcccgcg
      901 aaggcctggt gacgctcacg gaggcgctgc tcggcctcgg cgttgggtcg gcggcggcgc
      961 tggcgctcgc gatcgtgatg acccattcgc gcttcctcga gcgagggctc tatccgctcg
     1021 cgatcgggct caaggtgacg ccggtcgtcg tgtgggcgcc cctcttcgcg ctttggttcg
     1081 gcttcgggac gacgccaaag gtgttcgtcg cggccctcat caccttcttc cccatgctcg
     1141 tgaacacggt ggtagggctg cgcgatgtcg accccgacgc gcacgcgttt ctccgatcga
     1201 cgggagcgag ccgcgtcgaa gtgctctggt tcctccgctt gccctacgcc ctgccctacg
     1261 tctttgcggg acttcgcatc tcggcgcttc tcgcggtcat cggcgcggtc gtcgccgagt
     1321 ggtccggcgc agacgacggc atcggccatg ccattgctgt ggcgaacaac aacctggaca
     1381 cgccgacggt gttcgcgggc atcatcacgt tggccgtgat aggagcaatc ctgaatgcga
     1441 tactcgccct cgccgagcgt ctcgtcatcg actggcacgc gacccaaagg gcctagccgg
     1501 cgcccacgca ccctcctggc gttgtgtacg atcgccgttg cggtcattgc cctcgcctgc
     1561 ggcgatgacg atgacgccac cgcgggcacc gcgacggccg gcgatgctgc gcccgaacgc
     1621 gccgccatga cgttcctcgc cggcttccgt ccgcaggcca acctcccctt cgtcggcgca
     1681 tacgtggcgc aggagaacgg gtacttcgag gaggagggcc tcgacgtgac gatcgagcac
     1741 tccgagggcg ggccatcgaa ccttcagctc gtggcgcagg gccgcgtcca ggtcacaact
     1801 gccgatgcgg cgatcctgct gcagcgccgc gccgatcccg gcctccccat cgtcgcgatc
     1861 gcgctcatcg gacagcgcgg ccagcaagga tgggccacct tggccgatgc cggtctggat
     1921 tctccagccg actgggcggg caagcgtgtc ggctaccgca acgtcatacc gcccgacctc
     1981 cccgcaatcc tcgatgcgaa cggcctgtcg ctcgccgatg tggaagcgac cgacatcggc
     2041 tttcagcccc cgcagctgct cgtcgaaggc atcgtagacg tctaccccgt cttcctctcc
     2101 aacgagcccg acacgatccg tcgcgtccta ggacgcgagg ttcgcgtcgt gagcgcggcc
     2161 gactacggag tccccacgct cggactcacg tacgtcgcgt ccgaggagta tgcgcaggag
     2221 cacgccgacg aactctcgcg c
//
LOCUS       DATMBB010000170         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_278456, whole genome shotgun sequence.
ACCESSION   DATMBB010000170 DATMBB010000000
VERSION     DATMBB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278456"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VNL92_03515"
     CDS             complement(<1..206)
                     /locus_tag="VNL92_03515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HWO93809.1"
                     /translation="MVYIASGIIGVLALMAFIVPSQLAVIWIERRAIGRMQIRLGPNR
                     VGPFGLLQPIADALKVLVKEALTPP"
     gene            complement(307..1416)
                     /locus_tag="VNL92_03520"
     CDS             complement(307..1416)
                     /locus_tag="VNL92_03520"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWO93810.1"
                     /translation="MASSLQTEPFIVNIGPQHPSTHGVFRIRVAVDGEVITDADMVMG
                     YLHRSMEKLAEERTYTQNIPFTDRLDYLAAMSNNLAYVLAVEKLAGIETPERGQYLRV
                     IMAEFQRIASHCMAVGTFVNDCGAWQTPLMYMFKQREQILDLFEMTCGARITCNYVRI
                     GGVSYDIPEEFLPRAWALVNEMPSVLDEYEDLLANNEILLARSKGVGVLTAEEAINLS
                     CTGPMLRGSGVAWDLRKADPYCGYERFNFDIPVGRNGDCYDRFRVRLEEMRQSVNILR
                     QALEQLPGGPSTNPVELAFRPPVGEAYRGIEGPRGEIGFYVVSDGGPAPYRLHVRAPS
                     LINLSALPKMTIGSTIADAIVILGSVDIVVGEVDR"
     gene            complement(1441..1968)
                     /locus_tag="VNL92_03525"
     CDS             complement(1441..1968)
                     /locus_tag="VNL92_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWO93811.1"
                     /translation="MTREVPGPGLAEAIARAFEGAVADIQSGWVELSPDRLLPVMRWL
                     HDAPEHDFRQLLSLSAVDRLRRFEVVYHLVSHRRNHMACVKVGVTDREAPEVDSVTPI
                     WAGANLQEREAYDLMGIRFRGHPDLRRLFLWEGFAGWPLRKDFLQIPGKHPGLARFPH
                     EVPGQELDLTPRGTI"
     gene            complement(1965..2339)
                     /locus_tag="VNL92_03530"
     CDS             complement(1965..2339)
                     /locus_tag="VNL92_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWO93812.1"
                     /translation="MLQEFGHASFLLIVGIIFPLGAIVTSWAFTYVRIRPQVPNPVKE
                     ETYECGVPSEGTAWVQFNPRYYLFALLFLVFDVEILFLFPWAAAFDALGTKGFVAAMV
                     FIGILIVGFIYDWKKGALEWVR"
BASE COUNT          503 a          810 c          766 g          447 t
ORIGIN      
        1 ggcggcgtca gcgcttcctt taccagcacc ttcaaggcat cggcaatggg ttgcaacagc
       61 ccgaatgggc cgacgcgatt cggacccagc cgaatctgca tgcgaccgat ggctcgccgc
      121 tcgatccaaa tgaccgcgag ctggctgggc acgatgaacg ccatcagcgc gagcacgccg
      181 atgatccccg aggcgatgta caccacctcc gcgctgcccc agtcgtcgac ccaatcgagg
      241 atctcgcgcg ccgcgttccc gaggttgcgg aagtcgtacc aattgtccgt tacgagggca
      301 cgcacgctaa cgatcgacct cccccaccac gatatccacg cttccgagga tcacgatcgc
      361 atcggcaatg gtcgagccga tggtcatctt cgggagggcg gagagattga tgagcgaggg
      421 cgcgcgcacg tgcagccgat agggcgccgg gccaccgtcg ctcacaacgt aaaagccgat
      481 ctcgccccgt ggcccctcga tgcctcgata cgcctccccg acgggtggcc gaaatgctag
      541 ctctaccgga ttcgtcgacg gtccgcccgg aagctgctcc agcgcctggc gcaggatgtt
      601 cacgctctgc cgcatttctt cgagacgaac gcgaaaccga tcgtagcaat cgccgttcct
      661 tccgaccggg atgtcgaagt tgaatcgctc gtagccgcag tatgggtcgg ccttgcgcag
      721 atcccatgcg acgccgctcc cccgaagcat gggccccgtg cacgacaggt tgatggcctc
      781 ctccgccgtg agcacaccga cacctttgga acgtgcgagc aatatctcgt tgtttgcgag
      841 aaggtcctcg tactcgtcga gcacgctcgg catctcgttg accagcgccc aggcgcgcgg
      901 cagaaactct tccggaatgt catacgacac gccgccgatg cgaacgtagt tgcaggtaat
      961 gcgcgctccg cacgtcatct cgaacaggtc gaggatctgc tcccgctgct tgaacatgta
     1021 catcagcggc gtctgccatg cgccgcaatc gttcacgaac gtgccgacgg ccatgcagtg
     1081 gctggcgatg cgctggaact cggccatgat gacacggagg tattgacctc gctccggtgt
     1141 ctcgatgccg gcgagcttct cgacggcgag cacgtaggcg aggttgttcg acatcgcggc
     1201 gaggtaatcg agccggtcgg tgaagggaat gttctgcgtg taggtacgct cctccgccag
     1261 cttctccatg ctgcggtgga ggtagcccat caccatatct gcatccgtga tgacttcgcc
     1321 gtcgacagcc acccgaatcc ggaacacccc gtgggtggac gggtgctgcg gcccgatgtt
     1381 gacgatgaac ggctcggtct ggagcgacga cgccacagtc gagcatcctc ctatcgcgcg
     1441 tcagatggtc ccgcgagggg tcaggtccag ttcctggccg ggaacctcgt gaggaaaccg
     1501 cgccaggccg gggtgcttgc ccgggatctg caggaaatcc ttcctcagcg gccagcccgc
     1561 gaatccctcc cacaggaaca ggcggcgaag atcggggtga ccccgaaatc ggatccccat
     1621 gaggtcgtac gcctcgcgct cctgcagatt cgcgcccgcc cagatgggcg ttaccgaatc
     1681 cacctcggga gcctcacgat cggtcacccc taccttgacg cacgccatgt gattccttcg
     1741 atgcgacacg agatggtaga ccacctcgaa cctgcgcagc cggtcgacgg cgctcaagct
     1801 caggagctgc cgaaaatcgt gctctggagc atcgtgcagc cagcgcatca cgggcagtaa
     1861 ccggtcaggc gatagctcta cccatccgga ttggatatcg gcaacggcgc cttcgaatgc
     1921 gcgggcgatg gcttccgcta gcccgggtcc cggtacttcg cgcgtcacct gacccactcc
     1981 aaggcgccct tcttccagtc gtagatgaag ccaacgatga ggatgccgat aaacaccatc
     2041 gcggcgacga atcccttcgt cccgagggcg tcaaacgcgg ccgcccaggg gaagagaaac
     2101 agaatctcga catcgaaaac aaggaagagc agcgcgaaga ggtagtagcg aggattgaac
     2161 tggacccacg cggtgccctc cgatgggacg ccgcactcgt aggtctcctc ttttacaggg
     2221 ttcggaacct ggggccgaat gcggacgtag gtaaatgccc acgaggtaac gatggcgccg
     2281 aggggaaaga tgatgccgac gatgaggagg aaggaggcgt gaccgaactc ctggagcatg
     2341 gatgcggctc ctcagtcggg gatgggcttt gccgcgacgc gaggggaatt gtgaatttcg
     2401 tccgcagcgg cgcgagtata gcacgcccct ccaaagcacg gcaacgctcc tatccctgat
     2461 acggttgatc tcgccatcta ccttgtatta ctatctagct gccatcacat cggaggtcca
     2521 tcatgc
//
LOCUS       DATMBB010000171         5486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_401984, whole genome shotgun sequence.
ACCESSION   DATMBB010000171 DATMBB010000000
VERSION     DATMBB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401984"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..288)
                     /locus_tag="VNL92_03535"
     CDS             complement(106..288)
                     /locus_tag="VNL92_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93813.1"
                     /translation="MALRSKSRHTRSRADAAAAVITAALILTIVLILLLAPGPDEPES
                     TSPDAPSPSYIGETWV"
     gene            411..1154
                     /locus_tag="VNL92_03540"
     CDS             411..1154
                     /locus_tag="VNL92_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO93814.1"
                     /translation="MADVHEVTEGVWSLGDVPRVNFYALQEGGRVTVIDAGLGGYWGR
                     LERGLLAMGRTLGDVEAIVLTHAHIDHIGIAERLRNEANAVVRVHSDDLDWALGRSSP
                     PLKMGAMFRHVPRVLPFLFYFLRNGGVRFNKVLEASAFGDGETLELPGRPRVIHMPGH
                     TPGECALLAESRRVLFSGDALVTMHPLSHARGPQVMGSTFDADSKLALESLRRLEGVE
                     ADLVLPGHGEPWSGGVEAAVTEARRRGSD"
     gene            1353..2453
                     /locus_tag="VNL92_03545"
     CDS             1353..2453
                     /locus_tag="VNL92_03545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWO93815.1"
                     /translation="MLRKLLLSLLFPALVAGAIGFAACGGDDDDDDPTATSEPTQVEP
                     TGEPTGESDGGEEIDSEDIIPMYEKTAVFILAEGFYGGASSGTGIVLDAEGNILTNNH
                     VVEGAGSLAVRDPETDQMIPARIVGRSPCDDLAVIQVRDPDNFVPAELGAVADLAPGS
                     QVYAVGFPSSPSEDFFDTRLSITGGLVSKLDAQMAYYGLQNMIQTDTALNHGNSGGPL
                     VDKFGRVVGVNTLGSAPDLQNTNYAISIDEARSVFEQLLEGRDIDWLGVNAEPNDPSF
                     EIDFGVPYVENTLVIIGVDPNSPLFEESWIEGDVIAFADGKALATPGDLCSVIRSKRP
                     GDEILLEGLGQFRNNQTQEITYDLYTTRITIP"
     gene            2596..3576
                     /gene="add"
                     /locus_tag="VNL92_03550"
     CDS             2596..3576
                     /gene="add"
                     /locus_tag="VNL92_03550"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWO93816.1"
                     /translation="MADLARDSVALSDLAELPKAELHVHLEGAIRPSTLEELCARSGL
                     AIPRKFANLPEFIEEYVLAWRVLDQPGDYARVIREYCEDAARCGVRYAEIEIAPGRRA
                     YDHLAEACEAALKQHDVVVRFVIGVGRTLPVEVGWRMLERMADLPGMVGIGLGGEEEG
                     FPPEPFAELFAEGIRRGLRSTPHAGEVAGPASIRGALDALGAERIQHGIRAVEDVDLL
                     AEIVRRRIALAVCPTSNVLLGAVATIERHPLRALWDAGALVSIHTDDPGLFQCDIVGE
                     YAIAGRLLELDRAGYAQLALNSVEGSFAPDDLKAKLRAEIAGWLGARPPG"
     gene            complement(3573..4286)
                     /locus_tag="VNL92_03555"
     CDS             complement(3573..4286)
                     /locus_tag="VNL92_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO93817.1"
                     /translation="MYGDGDPGNLGDELEAADMELSIIAVAGQPSEVEGTLRVLIQTT
                     RGDIPGLLTPCEGEPGAVIMVGGAYGGFDGPADSVYERLAAPLAQRGVSTLRIHYRRP
                     NDFEECVLDVLAAASFLKGIGAERIALVGHSFGGAVVVKAAQLSDRIVAIVSMSPQLF
                     GTRQVHLMSKPLLLIHGADDQVLDMAASQDIYDRANEPKELVILPDTGHTLYQGKDEV
                     YELLLDWIPKHVRWAPSPN"
     gene            complement(4306..5451)
                     /locus_tag="VNL92_03560"
     CDS             complement(4306..5451)
                     /locus_tag="VNL92_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWO93818.1"
                     /translation="MADLPASGIRRFFDLLSSMDGVISLGVGEPDFVTPWNIRDAAIR
                     SIEEGHTHYTSNYGIPALRHALSRSIRRHYGIEYDPESEILITSGVSEGLDLALRVVL
                     DPGDEVLVPDPAYVAYRPCVTLAGGVTVDVPTTMEHAFTLQIDDLERLVTDRTKALII
                     NYPSNPTGAVMSREELMYVADFAARHDLFVISDEVYDRLVYGVGHTCFPSLPDMRHRT
                     VLLGGFSKAYAMTGWRLGWIAAPAAVLEAAMKVHQYVMMSAPTPSQYAALEALEHGEE
                     DVRGMLSEYERRRRVMVDGLNAIGLECFEPRGAFYAFPSVRSTGLDDETFAEQLLMEE
                     KVAVVPGSAFGVHGAGHVRCCYATALPEIWEALERMQRFVRKVRGSS"
BASE COUNT          907 a         1742 c         1880 g          957 t
ORIGIN      
        1 gtgggctccg ccgcggtggg ccgggcggat tgtaccagaa acgcgggtgg cccatatgcc
       61 catatgggtg gatggttggc gcggtcaggg gagcgtctgt tccgcctaca cccacgtctc
      121 gccgatgtag gagggcgagg gcgcgtccgg cgacgtgctc tccggctcgt ccggcccggg
      181 cgcgaggagg aggatgagaa cgatagtgag aatcagcgcc gccgtgatga ccgcggccgc
      241 tgcgtcggcc ctggaccttg tgtgtctcga cttcgaacgt agcgccaagg ctcgattcct
      301 gattgaggat aggtgtatcc tatgcgcccg gctcgggtgg cagcgacaca atgggcgttg
      361 ccgagtccgc gcacagcgtt gcaggtacat gtcataagag gagtgcgttc atggccgacg
      421 tgcacgaggt gacggaaggc gtctggtcgc tcggcgatgt gccgcgggtc aacttctacg
      481 ccctccagga gggcggtcgc gtcacggtca tcgatgctgg gctgggcggc tactggggaa
      541 ggctggagcg aggcctgctc gccatgggcc gcacgctggg cgacgtcgag gcgatcgtgc
      601 tgacccacgc ccacatcgac catatcggga tcgccgagcg gctgcggaat gaggcgaacg
      661 ccgtggttcg cgtccatagc gatgacctcg attgggcgct gggtcgctct tcgccgccgc
      721 tgaagatggg ggcgatgttc cggcacgtgc cccgtgtcct cccgttcctc ttctactttc
      781 ttcggaacgg cggtgtgcgc ttcaacaagg tgctggaggc gtcggctttc ggcgatggcg
      841 agacactcga actgcccgga cgccctcgcg tgatccacat gccgggccac actccaggcg
      901 agtgcgctct gctcgccgaa tcgaggcgcg tgctcttctc cggcgatgct ctcgtgacga
      961 tgcatccgtt gagccacgcc cgcggccctc aagtcatggg ctcgacgttc gatgcggatt
     1021 ccaagctcgc gctggaatcg ctgcggcgcc tcgagggcgt ggaggcggat ctggtgctcc
     1081 ccgggcacgg cgaaccctgg agcggaggcg tcgaggcggc tgtcacggaa gcacgacgcc
     1141 ggggcagcga ttagtcaacc agcggcggtt gacccttact cgcagaatgc cgatgatcag
     1201 gaggacacag tggggagcgg tcgccccttt tgggcggccg catttggtgt tcgccgatgc
     1261 ggcatcggcg gagaggagga ccgcaccggg cacaagcgga tctcaatggc agactcaagc
     1321 gtcttcattc ttagggaatc gggagcacca ccttgttgag aaaactgctg ctttcactgc
     1381 tgttccccgc tcttgtggcg ggggccatag ggttcgccgc ctgcggcggc gatgatgatg
     1441 acgacgaccc gacggcaacg tcggagccga ctcaggttga gccaacgggg gaacccacgg
     1501 gcgagtctga tggcggggag gagatcgact ccgaagacat catccccatg tacgagaaga
     1561 ccgccgtctt catcctcgcc gagggcttct acggcggtgc gtcgtccggc acgggtatcg
     1621 tccttgatgc cgagggcaac attctgacga acaaccacgt cgttgagggc gccggatcgc
     1681 tggccgttcg cgatccggag accgaccaga tgatcccggc gcgcatcgtg ggcagaagcc
     1741 cctgcgacga cctcgcggta attcaggtgc gcgatcccga caacttcgta ccggcggagc
     1801 tgggtgccgt ggccgacctc gcgcccggat cgcaggtcta cgctgttggg ttcccatcgt
     1861 cgccttcgga ggacttcttc gatacgaggc tctccatcac gggcgggctc gtcagcaagc
     1921 tcgatgcgca gatggcctac tacggactcc agaacatgat ccagacggat acggccctca
     1981 accacggcaa ctccggcggt cccctcgtcg acaaattcgg ccgggtcgtg ggggtgaaca
     2041 cgctgggttc cgccccggac ctccagaaca cgaactacgc catctcgatc gacgaggccc
     2101 ggagcgtctt cgagcagctc ctcgaaggtc gcgatatcga ctggcttggc gtgaacgccg
     2161 agccgaacga cccaagcttc gaaatcgatt tcggggtgcc gtacgtcgag aataccctcg
     2221 tcatcatcgg tgtggacccg aacagccccc tcttcgagga gagctggatc gagggcgatg
     2281 tcatcgcctt cgccgatggg aaggcgctcg ccacgccggg cgacctgtgc tcggtcattc
     2341 gttccaagcg tccgggcgac gagatcctgc tcgaggggct tggtcagttc cgcaacaacc
     2401 agacgcagga gatcacgtac gatctgtaca cgacccgcat caccatcccg tagattccgc
     2461 atctcgactt gggatgggag cgaggaggcc gccggccggg cggcctcctc gcatgtaagg
     2521 acttggtgag caggcgcctc aggggcactg cgctgcctcg cgcgtcggga ggagccgggg
     2581 tagcatgtgg gcgtcatggc tgaccttgct cgcgactccg tcgctctctc cgacctcgcc
     2641 gagctgccca aggcagagct gcatgtacac ctcgagggag caatccgccc gtcgaccctc
     2701 gaggagctgt gcgcgcgctc cgggcttgct atcccgcgga agttcgcgaa cctcccggag
     2761 ttcatcgaag agtacgtctt ggcttggcgc gtgctggacc agcccggcga ttacgcacgc
     2821 gtgattaggg agtactgcga ggacgccgct cggtgcggcg tgcgctatgc ggagatagag
     2881 atcgcgccag ggcgccgcgc ttacgaccac ctggccgagg cgtgcgaggc cgccctcaag
     2941 cagcacgatg tggtcgtccg attcgtgatc ggcgttggca ggacactccc ggtcgaggtg
     3001 gggtggcgaa tgctggagcg catggccgat ctgccgggca tggtcggcat cggcctcggt
     3061 ggggaggagg agggcttccc gccggagccc ttcgcagagc tgttcgccga gggaatccga
     3121 cgcggccttc gttctacccc gcatgccggc gaggtggccg gccctgcttc gatccgtggg
     3181 gcgctcgacg cgctcggagc ggagcgcatc cagcatggga tccgtgctgt ggaagatgtc
     3241 gacctcctcg ccgagatcgt ccggcggcgc atcgcacttg cagtgtgccc cacctcgaat
     3301 gtcctgctcg gggccgtcgc cacgatcgaa cggcacccac tgagggctct atgggatgcc
     3361 ggggccctcg tctccatcca cacggacgat ccagggctgt tccagtgcga catcgtcggg
     3421 gagtatgcga tcgcgggccg cctgctcgaa ctcgaccggg cgggctacgc gcaactcgcg
     3481 ctgaactcgg tggaaggctc gttcgcaccc gacgatctga aggccaagct ccgtgcggag
     3541 attgcgggtt ggctcggggc ccggccgccg ggctagttcg ggctgggcgc ccaccgcacg
     3601 tgctttggga tccagtccag taggagctcg tacacctcgt ccttgccctg gtagagcgtg
     3661 tgaccggtat cgggcaggat gacgagctcc ttgggctcgt tggcgcggtc gtagatgtct
     3721 tggctcgccg ccatgtcgag cacctggtcg tccgcaccat ggatgagaag gaggggcttc
     3781 gacatcaggt gcacctggcg cgtcccgaac aactgggggc tcatcgacac gatcgccaca
     3841 atgcggtcgg acaactgcgc tgccttcacg acgaccgctc ccccgaagct atggccgacg
     3901 agcgcgatcc gctccgcgcc gatgcccttg aggaaggacg ccgctgccag cacatcgagg
     3961 acgcattcct cgaagtcgtt gggccgccgg tagtggatgc gcagcgtgct cacgcctcgc
     4021 tgagcgagcg gtgcggccag ccgttcgtac acggagtcgg ccggcccatc gaagccgccg
     4081 tacgctcccc cgaccatgat caccgcgccc ggctcgccct cgcacggcgt cagcagaccg
     4141 gggatgtcgc ctcgcgtcgt ttggataagc acgcgcaggg tgccttcgac ctcgctcggt
     4201 tgcccggcga cagcgatgat ggacagctcc atgtcggcag cttccagctc gtcgccaaga
     4261 ttgcccggat cgccgtcgcc gtacatggcg ccttccttgg gagattcagc tgctgccgcg
     4321 caccttacgc acgaaacgct gcatgcgttc gagtgcctcc catatctctg ggagggctgt
     4381 ggcgtagcag cagcgaacgt gaccggcgcc gtggacgccg aacgcggagc ccggcacgac
     4441 ggcgaccttc tcctccatga ggagctgctc ggcgaaggtc tcgtcgtcga ggccggtcga
     4501 gcggaccgag gggaacgcgt agaaggcgcc ccggggctca aagcactcga ggccgatcgc
     4561 gttgaggccg tccaccatca cgcggcgacg gcgctcatac tcgctgagca tgccgcgcac
     4621 gtcctcttcg ccgtgctcga gcgcctcgag cgccgcgtat tgactcgggg tgggcgccga
     4681 catcatcacg tactgatgca ccttcatcgc tgcttcgagg accgcggccg gcgcggcgat
     4741 ccatccaagg cgccagcccg tcatcgcgta ggctttcgag aagccgccga ggagcaccgt
     4801 gcgatggcgc atatcgggga gcgatgggaa gcacgtatgc ccgaccccat atacgagccg
     4861 gtcgtacacc tcgtcggaga tgacgaagag gtcgtgtctc gccgcgaagt ccgcgacgta
     4921 catcagctcc tcgcgtgaca taaccgcgcc cgttgggttc gatgggtagt tgatgatgag
     4981 cgccttcgtt cgatccgtca ccaggcgctc aaggtcgtca atctgcagcg tgaaggcgtg
     5041 ctccatcgtc gtgggcacgt cgacggtcac gccgcccgcc agcgtgacac acggtcgata
     5101 ggccacgtac gccgggtcgg ggacgaggac ttcgtcgcca gggtcgagga ccacgcggag
     5161 agcgaggtcg agcccctccg atacgcctga cgtgatgagg atttcgcttt ccgggtcgta
     5221 ctcgatgccg tagtgccggc ggatggagcg cgagagggcg tgacgcagcg ctggtatgcc
     5281 gtagttggag gtgtagtggg tatgcccctc ttcgatggag cgaattgcgg catcgcggat
     5341 gttccagggc gtgacgaaat cgggctcgcc gacgccgagc gagatgacgc cgtccatcga
     5401 ggacagcaag tcgaagaagc gccggatgcc ggacgcggga aggtctgcca cccgttgcga
     5461 gatcgcgccg cgcctcgccg cgaggc
//
LOCUS       DATMBB010000172         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_2278, whole genome shotgun sequence.
ACCESSION   DATMBB010000172 DATMBB010000000
VERSION     DATMBB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VNL92_03565"
     CDS             <1..739
                     /locus_tag="VNL92_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWO93819.1"
                     /translation="IVGLANGDARTAVNILESATNSATKDEAGRLHIDAALVSEVAQR
                     ALRHDRAGAAHYDTISAFIKSLRGSDPDAAVYWLARMLEAGEDPLFIVRRMVILAAED
                     VGLADPQALQMAVAAQQAVHFVGLPEGYLPMAEAAIYLAMAPKSNSALRAYQAALADV
                     RETRNDPVPLHLRNAVTGLMAREGYGRGYRYAHEHAGGWVKQQHLPDKLVDRRYYDPT
                     DRGFEAAFRELLAQRRSDRAPDSGDNA"
     gene            complement(773..1615)
                     /locus_tag="VNL92_03570"
     CDS             complement(773..1615)
                     /locus_tag="VNL92_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO93820.1"
                     /translation="MTTWSAWQPVDGFTDEEMLYDKKYRDRGGGIARLRFNRPHRMNA
                     FHADNLATFVRGIEDANRDPDIGVIVVTSMGDHFGTGGDVQWEAEGGLQRPMPNFDGA
                     LKNSRKPTIAAVKGYCIGGHNHLAYHCDFTIAADTAIFGQNGPRVASPAHGMIVGASA
                     YIMGVKRAKEMWMLCRQITAQRAYEMGLANEVVPLERLDETVDQWADELLDLVPDCLS
                     VVKQTFEWVEQALAPTSGTLLAMIQPNFFQQPYVREAQQAFFEKRKPNFWPNAKKAAD
                     VQGS"
     gene            complement(1618..2046)
                     /locus_tag="VNL92_03575"
     CDS             complement(1618..2046)
                     /locus_tag="VNL92_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93821.1"
                     /translation="MTIVLLWKDGTEDSLLGNLALAIESKKAGEDVTVIVTEEALAAM
                     AGKGYTWSQALKDRPSRMTILKAAKELGLPYHRDFDPRWTEPAKLVDAAHQAGIRLVA
                     DPIWVKLLGLDGNLPEAVERIETSDMLKTLREASVVVGGF"
     gene            complement(2197..>2440)
                     /locus_tag="VNL92_03580"
     CDS             complement(2197..>2440)
                     /locus_tag="VNL92_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93822.1"
                     /translation="VRGRVMSLLQLNMGVSQFLTLPLAAIAQATSIELLFPTLALVVM
                     VLVGAIVVGQRHRFGAAISGTRIGLDEPSVQPSRTG"
BASE COUNT          404 a          814 c          800 g          422 t
ORIGIN      
        1 catcgtcggg ctcgccaacg gcgacgcgcg tacggcggtc aacatcctcg aatcggccac
       61 aaattccgcg acgaaggacg aggccggccg cctccacatc gatgcggccc ttgtctccga
      121 ggtggcgcag cgcgcgctcc gccacgatag ggccggcgcg gcgcactacg atacgatctc
      181 cgcgttcatc aagtcgttac gtgggtccga cccggatgcg gcggtctact ggctcgcccg
      241 tatgctcgag gcgggcgagg acccgctctt tatcgtgcga cgcatggtca ttctcgccgc
      301 cgaggacgtc ggcctcgccg acccgcaggc gttgcagatg gccgtcgcgg cccagcaggc
      361 cgtacatttc gtcggcctgc cggaaggcta cctgccgatg gcggaggcgg cgatctacct
      421 cgcgatggcg ccgaagagca actcggccct gcgcgcgtac caagcagcgc tcgccgacgt
      481 gcgggagacc cgcaacgacc cggtcccgtt gcatctgcgc aacgccgtca cggggctcat
      541 ggcgcgcgag gggtacgggc gcggctaccg atacgcgcac gagcacgcgg gtgggtgggt
      601 gaaacagcag cacttgcccg acaagcttgt cgaccgccgc tactacgacc cgaccgaccg
      661 cggcttcgag gcggccttcc gcgagctgct cgcccagcgc agaagcgacc gagcacccga
      721 ttccggagac aacgcctagg cgccagcccg cctagtcgac gcggacgcct ctctagcttc
      781 cctgcacgtc ggcggccttc ttggcgttcg gccagaagtt cggcttgcgc ttctcgaaga
      841 aggcctgctg cgcctcccgc acgtacggct gctggaagaa gttcggctgg atcatggcga
      901 gcagcgtgcc cgaggtgggc gcgagcgcct gctccaccca ctcgaaggtc tgcttcacca
      961 cggacagaca atcgggaacg agatcgagca gctcatcggc ccactggtcg accgtctcgt
     1021 cgagccgctc gaggggcacg acctcgttgg cgaggcccat ctcgtaggcg cgctgtgccg
     1081 tgatctggcg gcacagcatc cacatctcct tggctcgctt cacgcccatg atgtatgcgg
     1141 acgccccgac gatcatgccg tgcgcggggc tggcgacccg aggtccgttc tgtccaaaga
     1201 tggcggtgtc ggccgcaatc gtgaaatcgc agtgataggc caggtggttg tgcccaccga
     1261 tgcagtagcc cttgacggcg gcgatcgtcg gcttgcggga gttcttcaac gccccatcga
     1321 agttcggcat gggccgctgg agcccgcctt cggcctccca ctggacatcg ccgcccgtgc
     1381 cgaagtggtc gcccatgctg gtgaccacga tgacgccgat gtcggggtca cggttcgcat
     1441 cttcgatgcc ccggacgaac gtcgccaggt tgtcggcgtg gaacgcgttc atccggtgcg
     1501 ggcggttgaa gcgtaggcga gcgatgccgc cgccgcgatc gcgatacttc ttgtcgtaga
     1561 gcatctcctc gtcggtgaag ccatcgaccg gttgccatgc ggaccaggtg gtcatgctca
     1621 gaaacctcct accaccacgc tcgcttcgcg cagcgtcttc agcatgtccg atgtctcgat
     1681 gcgctcgacg gcctcgggaa ggttgccgtc aaggccgagc agcttgaccc aaatcgggtc
     1741 agcgacgagg cggatgccgg cctggtgcgc agcatccacc agcttcgccg gctccgtcca
     1801 ccgcgggtcg aaatcgcgat ggtaggggag gccgagctcc ttcgctgcct tcagaatggt
     1861 catgcgcgag ggtcggtcct tcagcgcctg agaccacgtg tagcctttgc cggccatggc
     1921 cgcgagcgct tcctcggtca cgatgaccgt gacgtcttcc ccagccttct tggactcgat
     1981 ggcgagggcg aggttgccca gcagcgagtc ttcggtgcca tctttccaga gcaggacaat
     2041 tgtcatacgt cacccctgaa tctatgccgg acgtggttcc ctgcctaacg gtcgttttag
     2101 tcgcccgggc cgcgtcagtc tagggagtgg ccggaatgcc tgtcaagcac tgtggcgtgt
     2161 cgggacggct gagagcgaga agggctggac acggcctcat cccgtccgcg acggctgcac
     2221 tgagggttcg tccaggccaa tgcgcgtacc tgagatcgcc gctccaaacc ggtggcgctg
     2281 gcccaccacg atcgcgccga cgagcaccat gacgacgagt gcgagcgtgg gaaaaagcag
     2341 ctcgattgat gtggcctgcg cgatcgcggc gagggggagg gtgaggaact gagacacgcc
     2401 catgttgagc tgtaacaagc tcatcaccct gccgcgcacc
//
LOCUS       DATMBB010000173         3761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_101195, whole genome shotgun sequence.
ACCESSION   DATMBB010000173 DATMBB010000000
VERSION     DATMBB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101195"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..642)
                     /locus_tag="VNL92_03585"
     CDS             complement(64..642)
                     /locus_tag="VNL92_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWO93823.1"
                     /translation="MIIDSINQQIIRILQANGRSTNRDIARQIGVSEGTVRNRIERLT
                     SEGVIRIAAVINPVKVGLQTTALMNLQVEPVHLTEVGEQLGRRGELTYIGYATGSADI
                     VCLGQFEDNDALFAFLTNVVAKVPGIQKMDTAIILHTVRRIFTNPEGVEVPPFPATKS
                     PDGASAVGRAPNTAPSRRRARQDGKRPRATRQ"
     gene            863..1900
                     /locus_tag="VNL92_03590"
     CDS             863..1900
                     /locus_tag="VNL92_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWO93824.1"
                     /translation="MTTMVRVAERAGELADRLGRYIQHRLSLAEPAEICGLVQIPGGA
                     SRETWSFDAAWEDEEGRHTWPLIVRRDPVAGLLETDRDREFAVYRALQGSSIPVPPVY
                     WLERDSAWLERPFFIMGRVPGIATPDAVLKGEFGSPEVIGHEMARILADIHMFDWTKH
                     DLEALGPPPAPNECASREVEHWQRIYERESFEPRPILDAAFRWLRAHPPTAERISLVH
                     GDYRTGNYLVTHDGITAVLDWEMAHLGDPMEDLGWTCMRFWRWARDDRVGGVLTREQF
                     FHRYEEAGGPRVDLDSVHYWELLGNLKMAIICITGARSFVEKRAVNATLAMVGRAVAP
                     LELELLDMMGL"
     gene            1903..2361
                     /locus_tag="VNL92_03595"
     CDS             1903..2361
                     /locus_tag="VNL92_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93825.1"
                     /translation="MRPHALQAIEGVQALLMQTVLPEMPGPYEQSQVTAAVGLLAMAA
                     VELDTLVEDLVAERQRLDVLLGEARTALGPLRDAGPLIQRIESAVGDEPGLRLSSLLA
                     AVEGRRALLGEVLARCEGGSEAAHARLRSRIYVEMRRRAEREQRVGIFAR"
     gene            2373..3488
                     /locus_tag="VNL92_03600"
     CDS             2373..3488
                     /locus_tag="VNL92_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93826.1"
                     /translation="MEDKYAEQYPLDPMDDYLIHQLPQPLRHVYTSDARAYERFWFTA
                     QDRVGELFLVVGGGIYPNLDTAEAYAIVVHEGQHTTVRHFRRLGRDRMNLTFGPIQLQ
                     VIEGLRTWRLQLQENDHGVSFDMLWHDTKRQVFDSMPGRYVRGRILAETAGFEGFGYP
                     EGTLTVGGRRFELSRERFMGSRDRHWGVRFGVGGPPHNPDVPAFAHGGQWVEFKDWSI
                     WGNRILYNFGDPRPGAGRIVKQDRKLRFDPQSHVFVEGVVTNVLEDGRTLDVHYERMN
                     NQTAYLRCGMYGGPTGGTPEGDIWHGMYVGDHVTQSETYDVNDRDVQARLAGLDDHHC
                     RVTCEGEVTYGIFEPIEPTAYEWCKAGVPGYSLLGEE"
     gene            complement(3501..>3761)
                     /locus_tag="VNL92_03605"
     CDS             complement(3501..>3761)
                     /locus_tag="VNL92_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93827.1"
                     /translation="AFARAHDEGWALAIVPRLVMRWARFGKSPMGRRVWADSVIRLPA
                     DAPTRWANALTAETVLATQRGPELVIDAADALRRLPVALLIA"
BASE COUNT          639 a         1162 c         1317 g          643 t
ORIGIN      
        1 gcgcgcccgc cgtcgggcga tgtgcaacgc tgttcgagcg tgaggtgctg acggaggcgg
       61 atttcattgc cgcgttgcgc gagggcgctt accgtcctgt cgtgctcgac ggcgagatgg
      121 cgcggtattc ggcgcccggc ctacagcgct agcaccgtcg ggcgatttcg ttgccggaaa
      181 cggcggcacc tcgacgcctt cgggattcgt gaaaatgcgc cgcaccgtgt gcaggatgat
      241 ggccgtgtcc atcttctgga tgcctggcac cttggccacg acgttggtta ggaacgcgaa
      301 gagggcgtcg ttgtcctcga actgaccgag gcagacgata tccgcgctgc ccgtggcgta
      361 cccgatgtac gtgagctctc cgcggcgccc gagttgttcc cccacctcgg tcagatgcac
      421 gggctccacc tgtaggttca tgagcgccgt tgtttgcagg ccgaccttca ccggattgat
      481 gacggcggcg atgcggatga cgccctcgct cgtgaggcgc tcgatcctgt tgcgcaccgt
      541 tccctcggat acgccgatct ggcgagcgat atcgcggttg gtcgagcgac cattcgcctg
      601 caggatgcgg atgatctgct gattgatgga atcgatgatc acagtggact cgaacgggga
      661 tgaggcaacg tgcgactgta caggacgcca tcgtacgaag gtgcggagac tatgtcaaac
      721 ctgatataga cgaagagttc gtgggacaga tgaaggaggc tgcagtcgcc ggcccaaggc
      781 gtctctgttt accaaacgtt gacgtgccac gccgagccgc ggtataccac tcgggcatat
      841 cgccgtcgtg caggagggga gcatgacgac catggtccgt gtcgccgagc gtgccggcga
      901 gctcgccgat cgcctggggc gctacattca gcatcgcctg tcgttggccg agccagcgga
      961 gatctgcggc ctcgtgcaga tccctggcgg ggcttcccgt gagacgtggt cgttcgatgc
     1021 cgcgtgggag gacgaagaag ggcggcacac gtggccgctg atcgtgcgcc gcgatcccgt
     1081 ggccggcctc ctcgaaacgg accgcgatcg cgagttcgct gtgtaccggg cgcttcaggg
     1141 ttcgagcatc ccggtgccgc ccgtctactg gctcgagcga gactcggcgt ggctggaacg
     1201 ccccttcttc atcatgggcc gggtacccgg catcgcgacg ccggacgcgg tgctcaaggg
     1261 ggagttcggg tcgccggagg ttatcgggca cgagatggcg cggatcctcg cggatattca
     1321 catgttcgac tggacgaagc acgacttgga ggcgctcgga cctccgccgg cgccgaacga
     1381 atgcgcctca cgcgaggtgg agcactggca gcgcatctac gagcgggagt cgttcgagcc
     1441 gcggcccatc ctcgatgcgg cgttccgatg gttacgggcg catccaccga cggcggagcg
     1501 catctccctc gtgcacggtg actaccgcac ggggaactac ctcgtgacgc acgacggaat
     1561 cacggcggtg ctcgattggg agatggccca cctgggcgat ccgatggagg acttgggctg
     1621 gacctgcatg cgattctggc ggtgggcccg cgacgatcgc gtcggaggcg tgctgacgcg
     1681 cgagcagttc ttccatcgct acgaggaagc tggcggaccc cgagtcgatc tggattccgt
     1741 gcactactgg gagcttctcg gaaacctcaa gatggcgatt atctgcatca cgggcgctcg
     1801 gtcgttcgtc gagaagcgtg ccgtcaatgc cacgcttgcc atggttggtc gggccgtcgc
     1861 cccactcgag ctggaattgc tcgatatgat ggggctgtaa tcgtgcgacc gcacgcgctg
     1921 caagcgatcg agggagtgca ggcgctgctt atgcagacgg tcctacccga gatgcccggg
     1981 ccgtacgagc agagccaggt gaccgcggct gtcggtctgt tggccatggc cgccgtggag
     2041 ctggatacgc tcgtcgagga cctcgtggcg gagcgccagc ggctggacgt gctcctgggc
     2101 gaggcccgca cggcgctggg gccgctccga gatgcggggc cgctcattca gcgaatcgag
     2161 tcggccgtcg gagacgagcc gggcctgcgc ctcagctcgc tgctggcggc ggtcgagggg
     2221 cgacgcgcct tgctcggcga ggtcctcgcc aggtgtgagg gcggcagtga ggctgcgcac
     2281 gcgaggctaa ggtcgcgcat ctacgtcgag atgcggcgcc gggcggaacg ggaacagcga
     2341 gtcggcatat tcgccaggta gggggccacg ccatggaaga caagtacgcg gaacagtacc
     2401 cgctcgaccc gatggatgac tatctgatcc accagctgcc gcaaccgcta cgccatgtct
     2461 atacgagcga tgcgcgggcg tacgagcgat tctggttcac ggcgcaggac cgagtaggcg
     2521 agctgttcct ggtggtcggc ggcggcatct acccaaacct ggacacggcc gaggcctacg
     2581 cgatcgtggt tcacgaaggc cagcacacga ccgtccgaca cttccgccgt ctcggtcgcg
     2641 atcggatgaa cctgaccttc ggccctatcc agctgcaggt gatcgagggt ttgcgaacct
     2701 ggaggctgca gttacaggag aacgaccacg gcgtcagttt cgacatgctc tggcacgaca
     2761 cgaagcgtca ggtgttcgat tccatgcccg ggcgctacgt gcgaggccgc atcctggcgg
     2821 aaacggcggg attcgagggc ttcggctatc ccgagggcac ccttaccgtc ggagggagac
     2881 gcttcgagct gagccgcgag cggttcatgg gctcccgcga ccggcactgg ggcgtgcggt
     2941 tcggagtcgg cggccctcca cacaatccgg acgttccggc gttcgctcac ggtggacagt
     3001 gggtcgagtt caaggactgg agcatctggg gcaaccgcat cctgtacaac ttcggcgacc
     3061 cgcgtccggg cgcggggcgc atcgtgaagc aggatcgcaa gctacgcttc gatccccagt
     3121 cgcacgtctt cgtggagggt gtcgtcacca acgttctcga ggacggccgg acgctcgacg
     3181 tacactacga aaggatgaac aaccagaccg cgtacctccg ctgcggcatg tacggcggtc
     3241 ccacgggggg cacgccagag ggcgacatct ggcacggcat gtacgtgggc gaccatgtga
     3301 cgcagtccga gacgtacgac gtcaacgacc gcgacgtgca ggcgagactt gccggcctcg
     3361 acgatcatca ctgccgcgtg acgtgcgaag gcgaagtcac ctacggcatc ttcgagccca
     3421 tcgagcccac ggcgtacgag tggtgcaagg cgggcgttcc aggctacagc ctcctcggcg
     3481 aggagtgagg cgagctcgcg ctacgcgatg agcagcgcga ccggcaggcg tcgcagcgcg
     3541 tcggcggcat cgatgacgag ttcagggccg cgttgcgtcg ccagcacggt ctcggcggtg
     3601 agggcgttcg cccatcgagt cggggcgtcg gcgggcagcc tgatgacgct gtccgcccac
     3661 acacgcctgc ccatcggaga cttgccgaac cgagcccagc gcatcacgag gcgcggcacg
     3721 atggcgaggg cccacccctc gtcgtgggcc ctcgcaaagg c
//
LOCUS       DATMBB010000174         3301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_300662, whole genome shotgun sequence.
ACCESSION   DATMBB010000174 DATMBB010000000
VERSION     DATMBB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300662"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VNL92_03610"
     CDS             <1..988
                     /locus_tag="VNL92_03610"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWO93828.1"
                     /translation="STLITMALRGAGAQAISLSGAQAGIRTDRRHSKARISGIDTTRI
                     RRELDAGRIVVVAGFQGVTEELDVTTLGRGGSDTTAVALAAALGAECCEIYTDVAGVY
                     TADPRIEPSARVLAEIGYEEMLELASQGAKVLHPRAVELGLLYEVPILVASSFDAGAG
                     TLVRKGTIVEAQNRVRGIAHDVDVARVTVRGVPDRPGIAAQLFGPLAEAGVSVDTIVQ
                     NSSLEGLTDLSFTVAKGELMHAMRVVEPVAREIGAADLVADASLGKVSVVGSGMVSGP
                     GYAATMFKTLFEAGVNIEMITTSEIRITCIVREDKVTEAVRALHAAYQLDIP"
     gene            1099..2466
                     /gene="lysA"
                     /locus_tag="VNL92_03615"
     CDS             1099..2466
                     /gene="lysA"
                     /locus_tag="VNL92_03615"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWO93829.1"
                     /translation="MTSAEFAAAATALPLTSIFPIDTSISSRGRLALGGCDTLALVER
                     FGSPLYVFDETTLRSQARAFVRAFGERYPDAIVRYAAKAHLTIAFAHLFADEGLSLDV
                     VSGGELAVALAGGFPAERIEFHGNNKTPQELDEALRAGVGRVAVDNLDEVRLLASIAE
                     RLGVRQAAILRVGPNVDPHTHGHTTTGLLDSKFGLPISTGQAEEAVRQMMASPHIELR
                     GIHAHLGSPIFETEPYRQAIDVMAAFAADMRYRYDFECLEFSPGGGFAVQYVRETPAP
                     EPDVYAEAIVAALRGAFEARDLPLPRLSIEPGRALVARAGVALYTVGARKEIPGVRTY
                     VSVDGGMADNIRPAIYGSRYEALVANRPLAAPEERVTIAGKYCESGDVLIRDIELPRL
                     VMGDVLAVPAAGAYCLAMSSNYNLALRPAVVLVREGRARLVQRRETYADLMSRDVMGA
                     EEPLE"
     gene            2463..>3301
                     /locus_tag="VNL92_03620"
     CDS             2463..>3301
                     /locus_tag="VNL92_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HWO93830.1"
                     /translation="MTWHTIGQERAIAALRNDLAGGQLAHAYLFTGPAQAGKATLALE
                     LAQALNCMADVDERPCQACTSCTRIARKHHADVEVFDSRSPCDVADHNHERDSTGAVR
                     ICQIRRVERQAYQAPFEGTTRLVIIDPADALTNEAANAFLKTLEEPPANTVLVLVAVD
                     AEGILPTVRSRCRTVALSAVPAPQLAAAMVAQWGASSEEADRLARMARGRPGWARTAL
                     EDEGVLLEHEALLDRIEALAAAPRDERLAYAESLAERWFSHRATVQEELAVWRAWWRD
                     VLV"
BASE COUNT          530 a         1038 c         1142 g          591 t
ORIGIN      
        1 cagtacactc ataacgatgg cccttcgtgg cgccggcgcg caggccatca gcctctccgg
       61 cgcgcaagca ggcatacgaa cggaccggcg tcacagtaag gcccgcatta gcggcatcga
      121 cacgacccgg attcgccgcg agctcgatgc gggacggatc gttgtcgtgg cggggttcca
      181 gggcgtcacg gaggagctgg acgtcacaac actgggacgc ggggggtccg atacgacggc
      241 cgttgccctg gcggcagcct tgggagcgga gtgctgcgaa atctacaccg acgtcgcggg
      301 cgtgtacacg gccgatcctc ggatcgagcc gagcgcccgc gttctcgcgg agatcgggta
      361 tgaggagatg ctcgagctcg cgagccaggg cgcgaaggta ctgcacccca gggcggtaga
      421 gttgggcctc ctctacgaag tccccatcct cgtcgcctcg agcttcgacg ccggggctgg
      481 tacgctcgtc aggaaaggaa cgatcgtgga agcgcaaaac agggttcgcg gcatcgcgca
      541 tgacgtcgac gtcgcccgag tgacggtgcg tggtgtcccc gaccgtcccg gcatcgcggc
      601 gcagcttttc ggtccgcttg ccgaagcggg cgtatctgtg gatacgatcg tgcagaactc
      661 gagcctcgag ggtctcacgg atttgtcctt cacggttgcg aagggcgagt tgatgcatgc
      721 gatgcgcgtc gtcgagccgg tggcaaggga aatcggcgcg gcggacttgg tcgccgatgc
      781 cagcctcggc aaggtcagcg tcgtcggctc gggcatggtg agcggcccgg gttatgcggc
      841 gacgatgttc aagacgctgt tcgaggcggg cgtgaacatc gagatgatca cgacgagcga
      901 gatccggatc acctgtatcg tgcgtgagga caaggtcacc gaggcggtcc gggcgctgca
      961 cgccgcctac cagctcgata tcccttagcc gtgcgccgtc cttttgcgcc ggcggatcgg
     1021 ccgggctata cttgggcggt agaagggcga agagcgccgt cgataccgcc ttcgtggtgt
     1081 ccggcgtaga tttccgaaat gacatcagcc gaatttgcgg cagccgccac ggccctgccg
     1141 ctcacatcga tctttccgat tgatacgagc atctcctcgc gggggcgtct ggccctcggc
     1201 gggtgcgaca cgctcgctct cgtcgagcgc ttcgggtcgc cactctacgt cttcgacgag
     1261 acgacgcttc gctctcaggc gcgcgcgttc gtccgcgcgt tcggtgaacg ctatcccgat
     1321 gcgatcgtgc gctatgcagc gaaggcccat ctcacgatcg cgttcgcgca cctcttcgcc
     1381 gacgagggac tgagcctcga cgtcgtctcg ggcggcgagc ttgccgttgc gctggccggc
     1441 ggcttcccgg cggagcgtat cgagtttcat ggcaacaaca agacgccgca ggagctggac
     1501 gaagcgctgc gcgccggtgt tggccgcgtc gcggtcgata acctcgacga agttcggctc
     1561 ctcgcttcga tagcagagcg gctcggcgtc cggcaagctg ccatcctgcg cgtgggcccg
     1621 aacgtcgatc cgcataccca tgggcacacg acgacgggcc tgctcgatag caagttcggg
     1681 cttcccatct ctaccgggca agccgaggag gcggttcgtc agatgatggc ctcgccacat
     1741 atcgagctgc gtggcatcca tgcgcacctt ggttcgccca tcttcgagac ggagccctat
     1801 cgtcaggcca tcgatgtcat ggcggcattc gcggccgata tgcggtaccg ctacgatttc
     1861 gagtgcctcg agttcagccc cggcggtggc ttcgcggttc agtacgtgag ggagacgcct
     1921 gctcccgaac cggacgtcta cgccgaggcg atcgtggcag cgcttcgggg cgccttcgag
     1981 gcgcgcgacc tcccgttgcc tcgcctctcg atcgagccgg ggcgggcgct cgtcgctcgg
     2041 gccggtgtcg ctctctatac cgtcggggcg cgcaaggaga ttccgggcgt gcgcacctac
     2101 gtctccgtcg atggtggcat ggccgacaac attcgtccgg ccatctacgg ttccaggtac
     2161 gaggcgctcg ttgcgaatcg tcccctggcg gcaccggagg agagggtgac catcgccggc
     2221 aagtactgcg aatcggggga cgtcctgatt cgcgacatcg agttgcctcg actcgtcatg
     2281 ggggacgtat tggccgttcc ggctgcaggt gcctattgct tggcgatgtc gagcaactac
     2341 aaccttgccc tgcgaccggc cgtcgttctc gtacgcgaag gccgggcgcg attggtgcaa
     2401 cggcgagaga cgtacgccga tctgatgtcg cgagacgtta tgggtgccga ggagccgctg
     2461 gagtgacgtg gcacaccatc gggcaggagc gtgccatcgc ggcgctgcgg aacgatctcg
     2521 ccggcggtca gcttgcgcac gcttatctct tcaccgggcc cgcacaggcc ggtaaggcca
     2581 cgctcgcgct cgaactggcg caggcgctca attgcatggc cgatgtcgat gagcgtcctt
     2641 gtcaggcatg cacgtcgtgc acacggatag cccgcaaaca tcacgcagac gtcgaggtct
     2701 tcgattcgcg ttcgccgtgt gacgtcgccg accacaatca cgagcgcgat tccaccggtg
     2761 ctgtacgcat ctgccagatc cggcgcgtcg agcgccaggc ataccaggcg cccttcgaag
     2821 ggacgactcg gctggtgatt atcgacccgg ccgatgctct gacgaacgag gcggcgaacg
     2881 cctttctcaa gacgctggag gagcctccag ccaatacggt cctcgtgctc gtagctgttg
     2941 atgccgaggg gatattgccc accgttcggt cgcggtgccg aaccgtcgcg ctgagcgcgg
     3001 tgccggcccc gcagcttgcg gccgccatgg tggcgcaatg gggcgcgtca agcgaggagg
     3061 cggaccggtt ggcgcggatg gcgcgcggcc gtccaggatg ggcgcgtacg gcgctagagg
     3121 acgagggcgt gctgctggag cacgaggccc tcctcgatcg gatagaggcg ctggctgctg
     3181 cgccgcgtga cgagcgcctg gcctacgccg aatcgctggc cgagcggtgg ttctcccacc
     3241 gggcaaccgt gcaggaggag ttggcggtgt ggcgcgcgtg gtggcgagat gtactcgtca
     3301 t
//
LOCUS       DATMBB010000175         4456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_85935, whole genome shotgun sequence.
ACCESSION   DATMBB010000175 DATMBB010000000
VERSION     DATMBB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85935"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VNL92_03625"
     CDS             <1..828
                     /locus_tag="VNL92_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93831.1"
                     /translation="SPFGQTGPYRDYVGTDLTLFHAGGMGRDTPFNEVTDLEAQPPLR
                     AGGSQAEYIAGWTAATLTMVAVEQRALTGRGQLVDLGILESVVNMMRYILADTTHGCV
                     PPLQRQKTSFGWVMPCKDGHVSFSPFNFDHWWDAFKEMMGRPDWAESELFSTAAGRIE
                     NADAIEPLATEWLLQRTKQEVYEMALARGVPCFPVNSMAEVVGSRQFRFREYFREVEH
                     PVAGRVVQPGPAIRFGSTPWQVRRPAPTLGQHTAEVICDELGYTRDELVMLRRTGAV"
     gene            857..2074
                     /locus_tag="VNL92_03630"
     CDS             857..2074
                     /locus_tag="VNL92_03630"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93832.1"
                     /translation="MTNRPLEGIRIADFGWVLAVPHATAWLGTLGAEVIRVESMARLD
                     IARAGGLARGADGEPGMNRGGMFNGLNFGKKGITLNLAHPKGLDLAKELIRRSDVVTE
                     NFGSGVFEKLGLGYDVVRELKPDIIMLSGSPLGSKGPDGAATGWGPNTQAYAGLPHIT
                     GYEGGPPSGLGGFYPDYMIGVVMAFALLTALHHRRLTGEGQFVELAMAETVAAMIPEA
                     ILDYTMNGRERERIGNYHESMAPHGVYRTKGDDTWVAIAVRGDDDWAAMRRAMGEPAW
                     AADPRFDDALGRRRHREELDRCIAEWTRQRTDYEAMHALQAAGVPAAPCSSAASLLED
                     PQVLERGTLPEIDHPETGPRRVVGLPGRLGALTWDYRRAPCFGEHNDDVYGGLLGLSR
                     DEIDRLVAEQVIF"
     gene            2209..2739
                     /locus_tag="VNL92_03635"
     CDS             2209..2739
                     /locus_tag="VNL92_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWO93833.1"
                     /translation="MDAIQGIRQGLDFIHRSFRSAADGLSDEQLHWSPEGESHSIAWI
                     LWHAARIEDLFVHQVFQGKPAEWDTGGWAAKTGLPERGFGTGQPTAEAKAVRIANLDA
                     FAQYAAKVADLADELVAGLTEDDLNREVKLGERTETLGQSITLHLITHLNGHRGEINI
                     LRGMMGLQPVLPNQGG"
     gene            complement(2753..3331)
                     /locus_tag="VNL92_03640"
     CDS             complement(2753..3331)
                     /locus_tag="VNL92_03640"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWO93834.1"
                     /translation="MLIDLHCHTRVHSPCSNLDPAELIALAKQAGLDGVCLTEHDRTW
                     DAADLAALAAEHAFLVLGGMEVTTEQGHVLVFGLDRYLPDMAIAGNLRRHVDRARGFM
                     ALAHPTRDEAHARRDPAVVRLFDAVEVMNGSDGRLRTASVGWGLPGIAGSDAHARHEV
                     GTCATRFDGAIRDERDLIQALRAGRFAPEALR"
     gene            complement(3338..4135)
                     /locus_tag="VNL92_03645"
     CDS             complement(3338..4135)
                     /locus_tag="VNL92_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO93835.1"
                     /translation="MAYQYALYEKKNHIAYITINRPEVRNALHPPANWELAEIWDDFA
                     NDPDAWVAILTGAGEHAFSAGNDLKYTAERGLGGGGEGERPMMSPSGFGGITSRFDLW
                     KPTIAAVNGFAVGGGLEMALSLDIIIAAEHAQLGLPEPKRGLIAGAGGIHRLPRAIPR
                     KIAMGMILTGKHITAQEAYRYGLVNEVVPLADLIPTAERWANEIIECSPVAVRASKQG
                     AVMGLEMPLQDAMRAQFDQMEAWRDSDDIREGPRAFAEKRKPNWSGR"
     gene            complement(4199..>4456)
                     /locus_tag="VNL92_03650"
     CDS             complement(4199..>4456)
                     /locus_tag="VNL92_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO93836.1"
                     /translation="FPILRGEQILVDPNDEALRTNIAIEHPGIAHEQVLVGAPWKYSK
                     TPTTLFRPTPRFAEHNHEVLSRLLGMTSEEIDRLVEAGVVA"
BASE COUNT          709 a         1444 c         1599 g          704 t
ORIGIN      
        1 agccccttcg gtcaaacggg gccctatcgc gattacgtcg ggaccgacct gaccctgttc
       61 cacgccggcg gcatggggcg cgatacgccg ttcaacgaag tcacggacct cgaggcgcaa
      121 cctccgctcc gtgctggagg ctcgcaggcc gagtacatcg cgggatggac ggccgcgacg
      181 ctcacgatgg tcgccgtgga gcagcgcgcg ctcaccggac gcggccagct cgtcgacctc
      241 ggcatcctcg agtcggtggt gaacatgatg cgctacatcc tcgcggatac gacgcatggg
      301 tgcgtgccgc cgctgcagcg acagaagacc agcttcggct gggtgatgcc gtgcaaggac
      361 ggccacgttt ccttctcgcc cttcaacttc gatcactggt gggacgcgtt caaggagatg
      421 atggggcgcc ccgattgggc ggaaagcgag ctcttctcga ccgctgccgg ccgcatcgag
      481 aatgccgacg cgatagagcc gctcgccacg gagtggctcc tccagcgcac gaagcaggag
      541 gtgtacgaga tggcgctcgc acgcggagtg ccctgcttcc cggtgaactc gatggccgag
      601 gtggtgggct cgcggcagtt ccgcttccgc gagtactttc gcgaggtgga gcaccccgtg
      661 gccggcagag ttgtccagcc gggcccggcc atccgcttcg gcagcacgcc ctggcaggtg
      721 cgccggcccg ctccgacgct cggtcagcac acggccgagg tgatttgcga cgagctggga
      781 tacacgcgcg acgagctcgt gatgctgcgc cgaaccggcg cggtgtagcg ggcgcggata
      841 cgaggggagc aacgccatga cgaatcgacc gctggagggc atccgtatcg ccgacttcgg
      901 ttgggtgctg gctgtgccgc atgcgacggc ctggctcggg acgttgggcg ccgaggtgat
      961 ccgcgtggag tcgatggcgc gcctcgatat cgcgcgggcg ggaggcctcg cgcggggggc
     1021 cgatggcgag ccgggaatga atcgcggtgg catgttcaac gggctcaact tcgggaagaa
     1081 ggggatcacg ctcaacctcg cccatccaaa gggcctcgat ctggccaagg agctcatccg
     1141 tcgcagcgat gtcgtgaccg agaacttcgg ctcgggcgtg ttcgaaaagc tgggccttgg
     1201 gtacgacgtc gtccgcgagc tcaagccgga catcatcatg ctgagcggct cgccgctcgg
     1261 ctcgaagggg ccggacggcg cggcgaccgg ctggggaccg aatacgcagg cttatgcggg
     1321 gctgccgcac atcacgggat acgaaggcgg gccgccatcg gggctgggcg gcttctaccc
     1381 cgactatatg atcggcgtgg tcatggcgtt cgcgctcctc acggccctcc accaccgccg
     1441 cctgaccggc gaggggcagt tcgtggagct ggccatggcc gagacggtgg ccgcgatgat
     1501 cccggaggcc atcctcgact acacgatgaa cggacgcgag cgcgagcgca tcggcaacta
     1561 ccacgagtcg atggcgccgc acggcgtgta ccgaacgaag ggcgatgaca cttgggtggc
     1621 catcgccgtc cggggcgatg acgactgggc ggcgatgcgg cgtgccatgg gcgaaccggc
     1681 gtgggccgcc gatccgcgtt tcgacgatgc gctgggacgc cggcgccacc gcgaggagct
     1741 ggaccgctgc atcgcggagt ggacgcgcca gcgaaccgac tacgaggcga tgcacgcgct
     1801 gcaggccgcg ggcgtgccgg ccgcgccgtg ctcctccgcg gcgagcctgc tggaggaccc
     1861 gcaggtgttg gagcgcggga cgctgccgga gatcgaccat ccggagacgg ggccgcggcg
     1921 cgtcgtgggg ctgccgggcc ggctgggcgc gctgacgtgg gactatcgcc gggcgccctg
     1981 cttcggcgag cacaacgatg acgtctacgg cggcctgctc ggcctctcgc gggacgagat
     2041 cgaccgcctc gtcgcggagc aggtcatctt ttgaggcacg gcgtagcaaa agccggatat
     2101 catacccccg gctggccgcg ctcccctctc cattgataac cgacatggtc aggggcgacg
     2161 cccactcgca taggcgggcg aagtgtgctg cagcggaagg agggaacgat ggacgcgatt
     2221 cagggcattc ggcagggtct cgatttcatc caccggtcgt ttaggagcgc cgccgatggc
     2281 ctctcggacg aacagctgca ctggtcgccc gagggcgaga gtcattcgat cgcctggatc
     2341 ctgtggcacg cggcccgcat cgaggaccta ttcgtccacc aggtgtttca gggaaagccc
     2401 gccgaatggg acactggcgg atgggcggcg aagactggac tcccggaacg cgggttcggc
     2461 acgggtcagc cgacggccga ggcgaaggct gttcgcatcg ccaacctcga tgccttcgcg
     2521 cagtacgccg cgaaggtcgc cgacctcgcc gatgagctcg tcgcgggcct cacagaggac
     2581 gacctcaacc gcgaagtgaa gctgggcgaa cggacggaga cgctgggcca gtcgattacg
     2641 ctgcatctca tcacgcacct gaacggccac cgcggcgaga tcaacatcct gcggggcatg
     2701 atgggcctac agcccgtgct gccgaatcag gggggctgag ggcgagggcg attcagcgca
     2761 gagcctcggg ggcgaagcgg ccggcgcgga gggcctgaat cagatcgcgc tcgtcgcgga
     2821 tggcaccgtc gaaacgcgta gcgcaggtgc ctacctcgtg ccgcgcgtgc gcatcgctcc
     2881 ccgcgattcc cggcaagccc catcccacgg acgccgtacg caacctccca tcactgccgt
     2941 tcatcacctc caccgcgtcg aagaggcgga cgacggccgg gtcgcggcgc gcgtgcgcct
     3001 catcgcgggt cggatgggct agggccatga agcctcgggc acgatcgacg tgacggcgaa
     3061 ggttgcccgc gatagccatg tctggtaggt agcggtcgag cccaaagacg agtacatggc
     3121 cctgctccgt ggtcacctcc atgccgccca gcaccaagaa ggcatgctcg gcggcgagcg
     3181 ccgcgaggtc cgccgcatcc cacgtgcgat cgtgctcggt gaggcacacg ccatcgagcc
     3241 cggcctgctt cgcgagggcg atgagctcgg ccgggtcgag gttgctacag ggcgagtgga
     3301 cgcgcgtgtg gcagtggaga tcgatgagca accgcggtca tcgtcccgac cagttcggct
     3361 ttcgcttctc ggcgaaggcg cgcgggccct cgcgaatgtc atcggagtcg cgccacgctt
     3421 ccatctggtc gaactgggcc cgcatcgcgt cctgaagcgg catctccagg cccatgaccg
     3481 cgccttgctt gctcgcgcgc accgcaacgg gcgagcactc gatgatctcg ttcgcccagc
     3541 gctcggcggt ggggatgagg tcggcgaggg ggacgacctc gttgacgagg ccgtagcggt
     3601 aggcctcctg cgccgtgatg tgcttgccgg tgaggatcat ccccatcgcg atcttgcgag
     3661 ggatggcgcg cggcaggcgg tggatgccgc ccgcccccgc gatgaggccg cgctttggct
     3721 ccggcagtcc gagctgggcg tgctcagcgg cgatgatgat atcgagggac agggccatct
     3781 cgagtccgcc gcccacggcg aagccgttga ccgccgcgat ggtcggtttc cacaggtcga
     3841 agcggcttgt aatgccgccg aaaccgctgg gcgacatcat ggggcgctcg ccctcgccgc
     3901 ccccgccgag cccgcgctcg gccgtgtact tgaggtcgtt gccggcggag aaggcgtgtt
     3961 cccccgcgcc ggtgaggatg gccacccagg cgtccggatc gttcgcgaag tcgtcccata
     4021 tctcggcgag ctcccagttg gcaggcggat ggagcgcgtt gcgcacctcg ggacgattga
     4081 tcgtgatgta ggcgatgtga ttcttcttct cgtagagcgc gtactggtag gccatcgttt
     4141 cgtctccttg tcgaagggcg tctgcggcga cggtgcgggt cggtcgccgg accgcgcgct
     4201 atgctaccac accggcctcg acgagccggt cgatctcctc ggacgtcatg cccagcaggc
     4261 gcgacagcac ctcgtgattg tgctcggcga agcgcggtgt gggccggaac agcgtcgtgg
     4321 gcgtcttgct gtacttccag ggcgccccga cgagcacctg ctcgtgcgcg atgccggggt
     4381 gctcgatggc gatgttcgtg cgcagggcct cgtcgttcgg atcgacgagg atctgctccc
     4441 cgcgcaggat cgggaa
//
LOCUS       DATMBB010000176         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_241066, whole genome shotgun sequence.
ACCESSION   DATMBB010000176 DATMBB010000000
VERSION     DATMBB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241066"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VNL92_03655"
     CDS             complement(<1..720)
                     /locus_tag="VNL92_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWO93837.1"
                     /translation="MTSAHPSIDVLAGLNPPQREAASHVSGPLLILAGPGSGKTRVIA
                     HRIAYLLGHEAVPPRRVFAVTFTNKAARELRDRVATLVGAAIDDLTLGTFHAVCARWL
                     RIDGERIGLERGFAIYDDADQIALMKSLLVEVGVDPNRNSPRAVLSAISRAKSELIDP
                     RAYESRVASYFEEIVSRVYPRYQAALRQNNAVDFDDLLTRTVELLREHADLLDKYRER
                     YLHVLVDEFQDTNVAQYQLAKL"
     gene            complement(799..1179)
                     /locus_tag="VNL92_03660"
     CDS             complement(799..1179)
                     /locus_tag="VNL92_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWO93838.1"
                     /translation="MTPSSDDLRIECISAVTLATHDMARSVRFYRALGFDLKYGGESA
                     GFTSFRLGSGHLNLVVPAPDQRWDWWGRIIFYVSDVDAMYRRAVAAGLQPEAAPRDAE
                     WGERYFHLVDPDGHELSFARPVPA"
     gene            complement(1176..1574)
                     /locus_tag="VNL92_03665"
     CDS             complement(1176..1574)
                     /locus_tag="VNL92_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93839.1"
                     /translation="MVASQKAGWEDLLRYFRIYAGPDGESHFGDVDVPFATTLGKSEL
                     SDTIAAEGIIFRRSAPDEHIDWHPAPRRQFVITLEGEAEITASDGEVRRVGPGSVMLA
                     EDTTGRGHVTRTVGGRERLSIFVVLADDAL"
     gene            1624..2148
                     /locus_tag="VNL92_03670"
     CDS             1624..2148
                     /locus_tag="VNL92_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWO93840.1"
                     /translation="MQFNVAQLLKEVVGSTRSYQVGSDLQMDGEVRHVSGSLEMIRTD
                     AGILVRAEVVVPAEAECSRCLSPVPGRKRLILEEEFLPTVDVVSGTRVNRDRDPEAFT
                     IDENHILDVSEAIRQYWVLAEPMHPLCKETCAGLCAICGRNRNIERCDCRTDDERWST
                     LAELAGALRESERS"
     gene            2150..2347
                     /gene="rpmF"
                     /locus_tag="VNL92_03675"
     CDS             2150..2347
                     /gene="rpmF"
                     /locus_tag="VNL92_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWO93841.1"
                     /translation="MTPLPKRRYPKARQGARRSHLRAPKVNIIECPHCHTPRRAHHAC
                     PTCGWYAGREAIRLREPELPG"
BASE COUNT          460 a          831 c          816 g          426 t
ORIGIN      
        1 gagctttgcg agctggtact gcgcgacgtt cgtatcctgg aactcatcga caaggacatg
       61 gagataccgc tcacggtact tgtcgagcag gtcggcgtgt tcgcggagca actcgaccgt
      121 gcgcgtgagc aagtcgtcga agtcaacggc attattctgg cgcagggcgg cctgatagcg
      181 cggatagacc cgcgacacga tctcctcgaa gtagctcgcg acacgtgact cgtaggcccg
      241 cggatcgatg agctcgctct tcgcgcgaga gatcgctgag agcacggccc gcggcgagtt
      301 cctgttcggg tccacgccca cctcgacgag gagcgatttc atgagggcga tctggtcggc
      361 gtcatcgtag atggcaaagc cgcgctccaa gccgatgcgc tctccatcga tcctgagcca
      421 tctcgcgcag accgcgtgga acgtccccag cgttaggtcg tcaatcgcgg caccgacaag
      481 cgtcgcgacg cgatcgcgca gctcgcgcgc cgccttgttc gtgaacgtca cggcgaagac
      541 gcgacgcgga ggcacagcct cgtgccccag cagatatgcg atccgatgcg caatgacgcg
      601 cgtcttgccg ctgcccggtc cagctaggat gagcagcggt ccgctgacgt ggctcgccgc
      661 ctcgcgctga ggcggattca ggcctgctag cacgtcgatc gatgggtggg ccgaggtcat
      721 ggcctcatgc taccatccga acgtatgttg tgatagcatc acgccttcgc tacacggcgt
      781 tcgaggagta gcccaacctc acgctggcac cggccgggcg aacgagagct cgtggccatc
      841 cgggtcgacg aggtggaagt accgctcgcc ccactcggcg tcgcgcggag ccgcctccgg
      901 ctgcaggccg gccgcgaccg cccgccgata catggcatcg acgtcgctca catagaagat
      961 gatgcggccc caccagtccc accgttggtc gggagcaggc accacgagat tgaggtgccc
     1021 ggagcccagc cgaaagctcg taaagccggc ggactcgccg ccgtacttga gatcgaaccc
     1081 aagggcgcgg tagaagcgaa ccgaccgcgc catgtcgtgc gttgcgagcg tcaccgcact
     1141 gatgcattcg attcggaggt catcggagct gggcgtcaca gcgcatcgtc ggcgagcacg
     1201 acgaaaatcg aaaggcgctc ccgtcccccg accgttcgcg tgacatggcc gcggccggtc
     1261 gtatcctcag caagcatgac gcttcccggc ccgacgcgcc ggacctctcc gtcgctggcc
     1321 gttatctccg cctccccctc gagcgtgatg acgaactggc ggcgcggcgc cgggtgccag
     1381 tcgatgtgct cgtccggggc actgcgccga aagatgatgc cttcggccgc gatcgtatcc
     1441 gatagctccg atttgcccaa tgtcgtggcg aacggcacat cgacgtcgcc gaagtgcgac
     1501 tcgccgtcgg ggcccgcata gatgcgaaag tagcgcaaga ggtcctccca tccggctttc
     1561 tgcgatgcta ccacttcgtc cttggcgatg aggacggatg ctacaatagc cgcgaggcgc
     1621 cgaatgcagt tcaacgtcgc gcaactgttg aaagaagtcg tcggctcgac ccgttcgtac
     1681 caggttggct ccgatctgca gatggacggc gaggtccgcc acgtcagtgg ctcgctcgag
     1741 atgattcgaa ccgacgcggg catcttggtc cgcgccgagg tggtcgttcc ggccgaagcc
     1801 gagtgcagcc gttgtctcag cccggttcca ggacgcaagc ggctcatcct cgaggaggaa
     1861 tttctgccga cggtcgacgt ggtgtccggg acacgcgtga atcgtgatcg tgacccggag
     1921 gccttcacga tcgacgagaa ccacatcctc gatgtctccg aggccatccg ccagtactgg
     1981 gtgctcgccg aaccgatgca tccgctctgc aaggagacgt gcgccggcct ttgcgcgatc
     2041 tgcggcagga accgtaatat tgagcggtgc gattgccgca cagatgacga gcgttggtcg
     2101 acgctcgcgg agctcgccgg cgcattgcgc gaatcggaac ggagttgaga tgaccccatt
     2161 acccaagcga cgctacccga aggcgcggca gggggcacgg cgcagccacc tccgggcgcc
     2221 caaggtcaac atcatcgagt gcccccactg ccacacgccg cgaagggccc atcatgcttg
     2281 tccgacgtgc ggctggtatg caggacgcga ggcgatccgt ctccgggagc cggagctccc
     2341 gggctagacc cggcatccgt gccctgctac cgttccccac atcttccaca gccatatcga
     2401 ccgcgcaaca ctgggcgcag cagctccgga gtcagatgat tgtcgcgacg ctggctaccc
     2461 aatagcgcgg ctcgtgccga cttggcgtgg ctgctgcccg gacaaggaac gcaggagccg
     2521 ggcatgggcc gtg
//
LOCUS       DATMBB010000177         2305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_337340, whole genome shotgun sequence.
ACCESSION   DATMBB010000177 DATMBB010000000
VERSION     DATMBB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337340"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VNL92_03680"
     CDS             <1..193
                     /locus_tag="VNL92_03680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWO93842.1"
                     /translation="VAVLTVDLDVSYLRAAREGNVIVDAVILRRTRRLAWGEASVTND
                     TGDLLARGRATFAILSQGT"
     gene            196..726
                     /gene="folK"
                     /locus_tag="VNL92_03685"
     CDS             196..726
                     /gene="folK"
                     /locus_tag="VNL92_03685"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWO93843.1"
                     /translation="MAQGDVYIGLGSNMGNRAENLRMALGLMPPLVRVEAVSSLYETA
                     PVGFEDQPHFLNAVARVTTGLQPLQLLRHLKGIETDIGRRPGPHWGPRPIDLDILLMG
                     DRVVRDAELVVPHPRMYVRSFVLAPLADLAPDLVPPEMDEPVTALRDAASMEGVRLVA
                     GPEWLERGRGSWLRWT"
     gene            complement(782..1033)
                     /locus_tag="VNL92_03690"
     CDS             complement(782..1033)
                     /locus_tag="VNL92_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93844.1"
                     /translation="MDRSKMTGAAIMALAAFQMIAFSFGALRRSYVAVAVPVATALAG
                     LSALAFWIGWTMVTTESDLEGPEFEEEFAATGGVGGSSS"
     gene            complement(1026..1244)
                     /locus_tag="VNL92_03695"
     CDS             complement(1026..1244)
                     /locus_tag="VNL92_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004596226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HWO93845.1"
                     /translation="MRRDLLDILACPICKAEFELAVDQENAEGIVSGTLTCTKCGERY
                     PIVDSIPNLLPPELRGELGATMKEKPHG"
     gene            1333..1974
                     /locus_tag="VNL92_03700"
     CDS             1333..1974
                     /locus_tag="VNL92_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93846.1"
                     /translation="MVRDETLWRPQTIAATTLPLEHGRIALHRREARLRPQRIDVRPS
                     REAIAAPLIGVLFGAACFAAIVVLRDTLPVVLLAGMLLVAVIALPLAGMGLVYSLIGA
                     NVVIDQRKNSVVFQQGVAGLGVGTREVVPFEKIAELVIEEAGQDDSDPLPTEELTQWQ
                     LTLVKTNGKRLPIAGMTSLRAYEMDALQPVVELGRAVATLTGKDLVLPDVEGL"
     gene            1976..>2305
                     /locus_tag="VNL92_03705"
     CDS             1976..>2305
                     /locus_tag="VNL92_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWO93847.1"
                     /translation="MTIYEDVLRAHKTIAVVGLTSDETRPSHRVAAYLQANGYRIIPV
                     NPNEQEVLGERAYPSLRDIPEHVEFVDVFRRPEHVPPVVDDAIAIGAKVLWMQDGIVH
                     EEAASKAR"
BASE COUNT          403 a          747 c          763 g          392 t
ORIGIN      
        1 ggtggccgtg ctcacggtcg atctcgacgt gagctacttg cgcgcggcgc gggagggcaa
       61 cgtcatcgtc gatgcagtca tcctgcgccg aacgcgccgc ctcgcgtggg gcgaagcgtc
      121 cgtaacgaac gacacgggcg atctgctcgc tcggggacgt gcgactttcg ccattctgtc
      181 gcaaggtacg tgaccgtggc ccaaggcgac gtctatatcg gcctgggatc gaatatgggc
      241 aaccgcgccg agaatctgcg catggccctg ggcctcatgc cgccgctcgt ccgcgtggag
      301 gccgtgtcgt cgctctatga gaccgcgccc gttggcttcg aggatcagcc gcatttcctc
      361 aacgccgtcg cacgcgtgac cacgggcctt cagcccctcc agctcctacg ccacctcaag
      421 gggatcgaga cggacatcgg gcgccggccg gggccgcact ggggcccccg cccgatcgat
      481 ctcgacattc tgctcatggg cgaccgggtc gtgcgcgatg ccgagctcgt ggtgccgcat
      541 ccgaggatgt acgtgcgctc gttcgtgctc gctccgctcg ccgacctcgc gccggacctc
      601 gtgcctccgg agatggacga gcccgtcaca gccctgcgcg acgcggcgag catggaggga
      661 gtgcggctcg tggccgggcc ggagtggctc gagcgtggcc gcggctcgtg gctacggtgg
      721 acctagaaag aagcacaacg gcaccgtctt ggcgccgttg tgaattcgac gaagcgaagt
      781 gctagctgct ggagcctccg acgccacccg tcgccgcaaa ctcctcctcg aactcgggac
      841 cctcgaggtc cgattcggtg gtcaccatgg tccagccaat ccagaaggcg agagccgaaa
      901 ggccggccag agcagtggcg acggggaccg ccaccgcgac gtagctgcgc cgcagtgcgc
      961 cgaagctgaa cgcgatcatc tgaaacgcgg cgagcgccat gatagccgcg cccgtcatct
     1021 tggacctatc catgtggctt ctccttcatc gtggctccca gctcacctct cagctccggg
     1081 ggtagcaggt tcgggatgga atcgacgatc gggtagcgct cgccgcactt cgtacacgtg
     1141 agcgtgccgc tcacgatccc ttcggcgttc tcctggtcga cagcgagctc gaactcggct
     1201 ttacagatag ggcaggcgag aatatcaaga aggtcacgtc gcatcgggcc cgccttcgtg
     1261 ctcgtctccg gacgctctgg ggcattgtag gcatcgcgga aaacgagtgt caacgcgggt
     1321 cgaccctatg ctatggtgag agatgagacg ctctggaggc cgcaaacaat cgcagcaacg
     1381 acgttgccac tggagcacgg tcgaatcgcg cttcaccggc gggaggcgcg tcttcgcccg
     1441 cagcgcatcg acgtgcgccc atcacgcgaa gcgatcgcgg ccccactcat cggtgtcctg
     1501 ttcggagcgg cgtgcttcgc ggccattgtc gtacttcgag acacgctgcc cgtcgtcctc
     1561 ctcgcgggca tgctgctcgt cgcggtcatt gccctaccgc tggccgggat gggcctcgtc
     1621 tacagcctca tcggcgcgaa tgtcgtgatc gaccagagga agaactccgt tgtattccag
     1681 cagggcgtgg ctgggctggg tgtcggcacg cgcgaggttg tcccattcga gaagatagcc
     1741 gaactcgtca tcgaggaggc cggccaggac gactcggacc cgctgccaac ggaagagctg
     1801 acccagtggc aactgacgct ggtaaagaca aacggcaaac gtttgcccat agccggaatg
     1861 acgtcgctcc gcgcctacga gatggatgcg ctgcagccag tcgtagagct cgggcgagcg
     1921 gtcgctacgc tcaccgggaa ggaccttgtc ctgcccgacg tggagggcct gtgaaatgac
     1981 catctacgaa gacgtcctgc gcgcgcacaa gacgatagcc gtcgttgggc tcacctcgga
     2041 cgagacgagg ccgagccatc gcgtggcggc ctacctgcag gccaacggtt accgcatcat
     2101 cccggtgaat ccgaacgagc aggaggtgct cggcgagcgg gcctacccga gcctgcgcga
     2161 cattccggag cacgtggagt tcgtcgatgt gttccgaagg cccgagcacg ttccgccggt
     2221 cgtagacgat gccatcgcca taggtgcgaa agtgctgtgg atgcaggacg gcatcgtgca
     2281 cgaggaggcc gcgtcgaagg cgcgc
//
LOCUS       DATMBB010000178         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_133391, whole genome shotgun sequence.
ACCESSION   DATMBB010000178 DATMBB010000000
VERSION     DATMBB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133391"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VNL92_03710"
     CDS             complement(<1..284)
                     /locus_tag="VNL92_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93848.1"
                     /translation="MSVMTVPVEYLDSVQPGRVAAVAGLGFTERQARFLVTVMVHSGV
                     FLERQYCSFAGISHGQKSHDFVAKLVDRRFATAITPGRLHQGRLFHVHYKP"
     gene            complement(281..910)
                     /locus_tag="VNL92_03715"
     CDS             complement(281..910)
                     /locus_tag="VNL92_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93849.1"
                     /translation="MTVSIAATTAWSTSSKGACFPPSRSTVTTAASRSTDRGRDLLEA
                     NRRERDDDRKQEFYAGVNRLRELSHDAQLYRAYERVEKELRDAGAEVRRVVLEQELKR
                     EYQSFLQDHNRDRPDSDGRPDRDPREIAQWALEHELPYFDDRVHFPDFRVEYDLDGRE
                     EHRDIEVLTPHYRGAHAAERGRTGFTCFRVRAGGRTGGWFDPRLAEELL"
     gene            1590..2318
                     /locus_tag="VNL92_03720"
     CDS             1590..2318
                     /locus_tag="VNL92_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWO93850.1"
                     /translation="MARLGVLRGGQRAATFQDFKRRLDSLRERNRLRGAAGLSQIGCI
                     VLVQAVFFSPEEWIPQPADWATRNLRYTRYDLTTAEGARIWQACQERLAARSAAAREA
                     FVAREERARYGEPALVRPRLGQGAFRVAVTDAYQRACAVTREHSLPVLEAAHIVPFAR
                     GGPHEVNNGLLLRSDLHRLFDLGYVTVTPELRLEVSGRLRGEFENGRTYYPMHGSDVS
                     VPADPRLHPDPHLLRWHNDTAFRP"
     gene            2352..>2760
                     /locus_tag="VNL92_03725"
     CDS             2352..>2760
                     /locus_tag="VNL92_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWO93851.1"
                     /translation="MLDDTQVRLAAFRFLEEQQRLAGEEGVLPRRVLVDGFTYEGHRV
                     PLMGPQGIFKPRVLREIPLSITTVAVVEGETRPYDDAFGDDGLLRYRYRGTDPRHHEN
                     VGLRLAMQRRVPLIYFHGIVPGLYVAEWPVYIVG"
BASE COUNT          446 a          936 c          899 g          479 t
ORIGIN      
        1 ggcttgtagt ggacatggaa caggcgcccc tgatggagcc gcccaggcgt gattgcggtg
       61 gcgaacctgc ggtcgacgag cttcgcgacg aagtcgtggc tcttctggcc gtgagagatg
      121 cccgcgaagc tacagtactg gcgctcgaga aatacccctg agtgcaccat caccgttacc
      181 agaaagcgtg cctggcgctc ggtgaaaccg agcccggcta cggccgccac acgccccggt
      241 tggacgctgt cgagatactc caccggcacc gtcatcacgc tcacagcaac tcctccgcca
      301 gccttggatc gaaccacccg ccggtacgtc cacctgcccg cacgcggaag caggtaaacc
      361 cggtacgtcc ccgttcggca gcgtgggcac cccggtaatg cggcgtcaga acctcgatgt
      421 cccgatgctc ctcgcgaccg tcgagatcgt actcgacgcg gaagtcggga aagtggaccc
      481 tgtcgtcgaa gtacggcagc tcatgttcaa gcgcccactg cgcgatctcc cgtgggtctc
      541 gatccggtcg tccatcgctg tctggccgat ctcggttgtg gtcctgcagg aacgactggt
      601 actcgcgctt cagttcctgc tcgagcacca cacgccgcac ctcggcgccc gcgtcgcgca
      661 gctccttctc cacccgctcg tacgcgcgat agagctgcgc atcgtgactg agctcgcgaa
      721 gccggttcac tcccgcatag aactcctgct tccgatcgtc gtcgcgttcc cggcggttcg
      781 cttcgagcag gtcgcgtccg cggtcggtgg agcgtgacgc cgcggtcgtg accgtcgagc
      841 gagatggtgg gaagcaagcc cccttcgacg aggtgctcca ggctgtcgtt gcggctatcg
      901 agaccgtcat cccgcgaatc gagatcacgc tcaggaatca cgcggaacgc gccaaccgct
      961 gcgagcatcc ggctgtcttc gccgttcagt tcgtagacgc gatcgcgatc gagaaccagc
     1021 tcgcgctcga ggccacgcgg caggtccagg tcgttgagca gcgcgtcgcg cggatcatca
     1081 gcgcgctcac gtgggtcgcg gtaagtgtcg cgatcgagtt cgccggcgcc gtctcgatcg
     1141 cgcgagtcgt tccaacgtgg atcgaagctg tcgaacatca tcgccccccc gagagaaggg
     1201 gacgacctct ctcgaaggcg agtatcagac gctccggatc gcgtcgcaat cgatgctcgg
     1261 gcggtgggcg gcggctttgg gtggtttcgg actccgtgtg ggccgcgttg caggcgacat
     1321 cgcaacgaaa aacggcgaaa agggcggaag ctgtcgtatc ctcgtgccgg gctcacaaca
     1381 cccatgcatg gctggatcgc cgtcacgcac agcgactggt tcgactttct cgcgcggcag
     1441 cgtcatcgcg aggaggtcaa cttctggagc ccgtcggact actacgcgtt ccacgggacg
     1501 ccgggcgccc cgttcttctt caagctgaag gcgccccgga atgcgattgg cgggttcggc
     1561 tacgtcgcga gcttctcgcg cctgcccgat tggctcgcct gggagtgctt cggggagggc
     1621 aacgggcggc caccttccag gacttcaagc gtcggctcga cagcctgcgg gagcgcaaca
     1681 ggctccgcgg cgcagcgggg ctgtcgcaga tcgggtgcat cgtgctcgtc caggccgtgt
     1741 tcttttcgcc ggaggagtgg atccctcagc cggcggactg ggcgacacgg aaccttcgat
     1801 atacgcgcta cgacctgacc acagccgaag gcgcccgtat ctggcaggcg tgccaggagc
     1861 ggctcgcggc gcgcagcgcg gctgcgcgtg aggcgttcgt tgcacgcgag gaacgcgccc
     1921 gatacggtga accggcactc gtgcgcccgc gacttggaca gggcgccttt cgcgtcgctg
     1981 taaccgatgc ctaccagcgc gcctgcgccg tcacgcgcga gcactccctg cccgtgctgg
     2041 aggccgccca catcgtccca ttcgcgcgag gcggaccgca cgaggtcaac aacgggctgc
     2101 tactccgctc ggatctccac cggttgttcg atctgggata cgtgaccgtc acgccggagc
     2161 tgcgcctcga agtgagcggc cgcctccgcg gcgaattcga gaacggccgc acgtactatc
     2221 caatgcacgg cagcgatgtg agcgtgccgg ccgatccccg gctgcatccc gatccgcacc
     2281 tcctccgatg gcacaacgac acggcgttcc ggccgtgagc tgtctcgctc gttggaatgg
     2341 agccgctcct gatgctggat gacacccagg tccgactggc ggcgttccga ttcctcgagg
     2401 agcagcagcg tctcgccggc gaggagggcg tcctgcctcg cagggtgctc gttgatggat
     2461 tcacctacga gggacaccgt gtgccgctga tggggccgca gggcatcttc aagccgcgcg
     2521 tcctccgcga gatcccgctc agcatcacga ccgtggcggt cgtcgagggc gagacgcgcc
     2581 cgtacgacga cgcattcggc gacgatggcc tgctgcggta tcggtaccgc gggaccgatc
     2641 cgcggcacca cgagaacgtc gggctccggc tcgcgatgca gcgccgcgtc ccgctcatct
     2701 acttccacgg catcgttccc ggcctgtacg tcgccgagtg gcctgtctac atcgtgggcg
//
LOCUS       DATMBB010000179         2967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_193539, whole genome shotgun sequence.
ACCESSION   DATMBB010000179 DATMBB010000000
VERSION     DATMBB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193539"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VNL92_03730"
     CDS             <1..431
                     /locus_tag="VNL92_03730"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HWO93852.1"
                     /translation="GDVPDPQAEETFRRSKLNRALQTEPRHAALLALHRELLRLRRDL
                     SVLRELERSRTHVAHDDSGTLVVRRWSEEGEFVAAFRLRAEPGNVALPLAGGPWRKLL
                     DSEEERWQGAGSAVPPTIDSAGSVVVSLAGWSFVMLYRER"
     gene            438..2864
                     /locus_tag="VNL92_03735"
     CDS             438..2864
                     /locus_tag="VNL92_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HWO93853.1"
                     /translation="MNRYVCIHGHFYQPPRENPWLEEIELQDSAHPYHDWNERINAEC
                     YAPNTASRILDGEGCIVGIINNYAHMSFNIGPTLLKWLEDRSTSTYERILDADRESAE
                     RFGGEGSAMAQAFNHAIVPLCNSRDKWTQVVWGIRDFQHRFGRSPRGMWLPETAVDLE
                     TLDIMARNGVAFTVLAPHQASRTRSMGDRAWVDVSGSRVDPHRPYQVRTPSGRRIAVF
                     FYDGPTSRAVAFEGLLTRGENFANRLLGAFDDSRDEPQLVHIATDGESYGHHHRHGDM
                     ALAYAMKFIESTENVRLTNYSQFLRMFPPKHEAQIFENTSWSCAHGIERWRSNCGCNS
                     GGRPGWNQEWRKPLRDALDWLRDTIAPSFEAAGRELLRDPWEARNDYIDVILNRDPDN
                     IERFYATHALRPLSEEERVRVLKLMELQRHALYMYTSCGWFFDDISGIETVQVIQYAG
                     RVLQLADDLFGDSLESRFLTQLERAKSNIPEHRDGRHIYEKWVKPAQVDLLKVGAHYA
                     VSSLFEDYSEQTKIYFFRAEREDYRRMEAGIAKLALGRVRLVSELTGESARAAFGVLH
                     FGDHNLSAGVRPYGDDASYQVIVEEISEVFSRADLPEALRLLDKHFLGVAYSLRSLFR
                     DEQRRVLNVILAATLAEAEANYRTVYEHHAPLMRFLADLGSPLPRAFGAAAELVLNSN
                     LRNELQSDNPSPESVRALLDEVALWNVQLGSEELGFVFQLTLDRLAERMNEAPLDVDV
                     LHALGDAVEVGSMLPFQVVPWRPQNMYYDLLHSLYPEMEARAAAGDEEAHTWLEPFKS
                     LGAKLQVRVP"
BASE COUNT          554 a          976 c          902 g          535 t
ORIGIN      
        1 aaggagacgt acccgatccc caggccgagg aaacgttccg tcgatcgaag ctgaaccggg
       61 ccttgcagac cgaaccacgc catgccgcct tgcttgcgct ccatagggag ctcctgcggc
      121 tgcgacgtga tctgtccgtc cttcgcgagc tcgaacgctc ccggacccac gtcgcccacg
      181 acgattccgg cacgctcgtg gttcgccgtt ggagcgagga gggcgaattc gtggcggcgt
      241 tccggcttcg ggcggagccc ggaaacgtcg cgctgccgct cgcgggcgga ccgtggcgta
      301 agctgctgga ctccgaagag gaacgctggc agggagccgg cagcgccgtt cccccgacca
      361 tcgactccgc cgggtcggtc gtggtttcgt tagccggctg gtccttcgtc atgctgtatc
      421 gggagcgcta gctgcatatg aaccgttacg tgtgcattca tggccacttc tatcagccgc
      481 cacgcgagaa cccgtggctc gaggagatcg agctccagga ttcggcgcat ccgtatcacg
      541 actggaacga gcgcatcaat gctgagtgct acgcaccaaa cacggcgtcg cgcattctcg
      601 atggcgaggg atgcattgtc ggcatcatca acaactacgc gcatatgagc ttcaacatcg
      661 gtcccacact cctgaagtgg ctcgaggacc gatcgacctc tacctacgag cgcatcttgg
      721 acgccgaccg cgagagcgcg gaacgcttcg gaggtgaagg gtcggcgatg gcgcaagcct
      781 tcaaccacgc catcgtgccg ctctgcaatt cgcgcgataa gtggactcag gtcgtgtggg
      841 gcatccgcga tttccaacac agattcgggc ggtcgccaag aggcatgtgg cttccggaga
      901 cagcggtcga cctggaaacg ctcgacatca tggctcgcaa cggcgtcgcc ttcaccgtcc
      961 tggctccgca ccaagcctcg aggacccgtt ccatgggcga ccgcgcctgg gtggacgtat
     1021 ccgggagccg cgtcgatcct caccggccct accaagttcg aaccccttcc ggccgccgca
     1081 tcgccgtctt cttctacgat gggccaacgt cgcgcgctgt cgccttcgag ggcctcctca
     1141 cacgcggtga gaacttcgcc aaccgcctgc taggcgcgtt cgacgacagc cgagacgagc
     1201 cgcaactcgt ccacattgcc accgatggcg agtcatacgg ccatcaccac cggcacggcg
     1261 acatggcgct cgcttacgcg atgaagttca tcgaatcgac ggagaacgtt cgtctcacca
     1321 actacagcca gttcctgcgc atgtttcctc caaagcacga ggcccagatc ttcgaaaaca
     1381 cttcctggag ttgcgctcac ggcatcgagc gatggcgcag caactgtgga tgcaactctg
     1441 gaggaaggcc tggatggaat caggagtggc gcaaaccact ccgtgacgcg ctcgattggc
     1501 tgcgcgacac gatcgcgccg agcttcgaag cggcggggcg ggaactgcta cgcgacccat
     1561 gggaagcccg caacgattac atcgacgtca tcctcaaccg tgatccagac aacatcgagc
     1621 ggttctatgc tacgcacgct ctacgtcccc tttcggagga ggaacgcgtg cgcgtgctga
     1681 agctgatgga gctccagcgt cacgccctct acatgtacac cagctgcggg tggttcttcg
     1741 atgacatctc cggcatcgag acggtccagg ttattcagta cgcgggacgc gtcctgcaac
     1801 tcgcggacga tctcttcggc gacagtttgg agtcgcggtt cttgacccaa ctcgagcgcg
     1861 cgaagagcaa tatcccggag catcgcgatg gacgacacat ctacgagaaa tgggtgaagc
     1921 cggcgcaggt cgatctcctc aaggtcggcg cgcactacgc ggtcagctcc ctgttcgagg
     1981 actactccga gcagaccaag atctacttct tccgggccga gcgcgaagac taccggcgga
     2041 tggaggcagg tatcgccaag cttgccctgg ggcgcgtccg gcttgtctcc gagctgacag
     2101 gggagtcggc ccgagccgcg ttcggcgtgc ttcacttcgg cgaccacaac ctgagcgccg
     2161 gggtgcgccc ctatggagac gacgcgtcgt accaggtcat cgtggaggag atcagcgagg
     2221 tgttctcgcg ggcggacctt cccgaggccc tccgcctgct ggacaagcac ttcttgggcg
     2281 tggcctactc gctccgatcg ctgttccgcg atgagcagcg tcgagtcctc aacgtgatcc
     2341 tggctgctac gctcgccgaa gcggaagcga actaccgcac ggtgtacgag caccacgcgc
     2401 cgctcatgcg cttcctcgcc gatctcggca gccccctgcc tcgggctttc ggcgctgccg
     2461 cggaacttgt gctgaactcg aacctccgca acgagctgca gagcgacaac ccctctcccg
     2521 aaagcgttcg cgcactcctc gatgaggtcg ccctgtggaa cgtgcaactc ggctccgaag
     2581 agcttggctt tgtgttccaa ctcacgctcg accgcctcgc cgagcgtatg aacgaggcgc
     2641 cactcgacgt ggacgtgctg cacgcccttg gcgatgcggt cgaggtcgga tccatgctgc
     2701 cgttccaagt cgtgccttgg cgtccgcaga atatgtacta cgacctcctg cactcgctct
     2761 atccagagat ggaggcgcgc gcggcggcgg gagacgagga agcgcacacc tggctggagc
     2821 cgttcaagtc cctgggcgca aagctccaag tgcgcgtccc gtagatgccc gattcgagac
     2881 acgtacctcg cgcaacgtat cgcgtgcaac tcacacgggg gttcggcttt gcggcagcac
     2941 gcaagattgt gccatacctc cgcgacc
//
LOCUS       DATMBB010000180        10188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_557058, whole genome shotgun sequence.
ACCESSION   DATMBB010000180 DATMBB010000000
VERSION     DATMBB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557058"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNL92_03740"
     CDS             complement(<1..217)
                     /locus_tag="VNL92_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWO93854.1"
                     /translation="MQRVLAFPAMTDRIRTVMRNTVWMLMSTALTRGLSYGYTILLAR
                     ALGVSDFGEYIFLITYITYFGLLADFGL"
     gene            complement(301..861)
                     /locus_tag="VNL92_03745"
     CDS             complement(301..861)
                     /locus_tag="VNL92_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWO93855.1"
                     /translation="MLARKIAPLEDVGLLLLRLITGVTLVVHGFPKLFGGPEKQPPKV
                     LVRAFGQNYPQAYEHGSPSGFAEALRQMNVPMPRVSAYLSGMAELFGGLALILGLMTR
                     FFAPMAAFNMAVAARVAHWQTGFYGPGGYEFPVLLGSAAGTLFFTGPGALSLDAAIDA
                     ASNRKERKRGRSFWDRRARRVLRARR"
     gene            1030..1656
                     /locus_tag="VNL92_03750"
     CDS             1030..1656
                     /locus_tag="VNL92_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93856.1"
                     /translation="MIKIDIGCGMSKHLGTLGVDIAPLAGVDVVADINTGLPFRDSVI
                     DEVHMSHTLEHVDDFLATMEEIWRVCKDGALFYIRLPHGSSSFTTWVDPTHKRGFNIQ
                     TFTYFDPEVNHLSYYSKARFKLVYSKLALSTLPAELNGTRLPRRVLSMVLESVANKSP
                     HWRARTERWFAQLIGFEEARVVLRAMKPGAPARREGPGLASASRRNGA"
     gene            1658..2737
                     /locus_tag="VNL92_03755"
     CDS             1658..2737
                     /locus_tag="VNL92_03755"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO93857.1"
                     /translation="MEDGTKPQPPQLRNVLVTGGAGYIGAVLVPKLVARGYNVRVLDR
                     FFWGEAAVRDVLPEKALINRDIREIEPSDLRGIDAVVHLAGLSNDPTAEFDPIANHEM
                     NAEASERLARACRESGVRRMTFGSSCSIYDGLPPGPIYDETAPVQPKGAYAMAKRYAE
                     ERILEISDPEATDNAGFSAVVLRQATVYGYSPRMRFDLVVNTFLKDALTQGKLFLHGG
                     GWMWRPLVDVADVAEAHIVALESPARTVAGEVFNVVHSNYQIRELAMIVAGSVQLVAG
                     RQVELEEAPMPRFRRDYRCSNAKLATATGFLPEVGVLESVQTMLERLRGIDARDFSHP
                     RYYNIEWMTLLTEMHEHLRGYARVL"
     gene            2739..3602
                     /gene="rfbD"
                     /locus_tag="VNL92_03760"
     CDS             2739..3602
                     /gene="rfbD"
                     /locus_tag="VNL92_03760"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWO93858.1"
                     /translation="MRVLVLGGHGQLASDITRLWTNLQPIPLGHQECDVTDRASIDGA
                     IAAHRADLVLNTAAYHKVDDCEGEGALAAFAVNVLGAKNVADACREADLPYAWLSTDY
                     VFSGSKGAPYREGDAPDPVSMYGVSKAAGEHAVRYANSRHYIIRTSGLYGIAGASGKG
                     GNFVETMLRLARERKPIRVVNDQRLAPTSTADLAIALERLLVTEAYGTYHITNAGECS
                     WYEFAGAIFAFTQAEVDFEPTTSNEFGAAARRPAYSVLANERLTSLGIEQPRPWREAL
                     EAYLGEKGYLR"
     gene            complement(3616..3981)
                     /locus_tag="VNL92_03765"
     CDS             complement(3616..3981)
                     /locus_tag="VNL92_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO93859.1"
                     /translation="MPLIVSCSRCQAPHDETDRFCSACGTPLQEDARPVPATESRAVV
                     PWRGPMPGIVQGMVLAGGGLALRFALAKAIPMMARVAMRRAVGRRVRQGPSRGRLPVV
                     QQPDYIIEQYTVRRVRFFR"
     gene            complement(4012..4830)
                     /locus_tag="VNL92_03770"
     CDS             complement(4012..4830)
                     /locus_tag="VNL92_03770"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HWO93860.1"
                     /translation="MTLQVGWFATARGAGSYNLLETTLRAIDSGSLDARIAFVFCNRE
                     PGESEATDRFFDLVRSRDIPLLTLSSVRFRKEREGERSRPTAPLPAWRREFDDALLRL
                     VAPFSIDVAMLAGYMLIFTEDACLRLPLLNLHPAAPGGPAGTWQQVIRELIETRARRS
                     GVTVFLATPEVDAGPPVTYCTFSLDDAALAPHWAEAAPRRGATLDETALFRAIRERGM
                     AREGPLVVETLRAFVQGERRVQAFRVVDAKGEDAPPLDLTERVEAALAGVHPML"
     gene            complement(4827..5525)
                     /locus_tag="VNL92_03775"
     CDS             complement(4827..5525)
                     /locus_tag="VNL92_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWO93861.1"
                     /translation="MTSIRFDLHTHTIFSPDSAITPDEFVRRCVAAGLDCVAVTEHDT
                     IDGALYVRDIAPFRVVIGEEVRSREGEIIGLFLRETVPPGLSAEETVMRIREQGGVVA
                     IPHPFDRFRDGLGEDAMLRILPEIDVIEVFNGRIRLARDNEHAERFARQYGITMAGGS
                     DAHSASEVGRTYVVMPDFETPAEFLDSLCSATIVGRRAPRWVYAMSSFEKLRRRAGWG
                     RTLPERPHTADTSR"
     gene            complement(5527..5736)
                     /gene="xseB"
                     /locus_tag="VNL92_03780"
     CDS             complement(5527..5736)
                     /gene="xseB"
                     /locus_tag="VNL92_03780"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWO93862.1"
                     /translation="MSSKDEERFEQVYERLQETVRRLEDGSLPLEEAIEAYETGMALV
                     ARCRDLLTQAELRVTRLREQLDSDG"
     gene            complement(5733..6941)
                     /gene="xseA"
                     /locus_tag="VNL92_03785"
     CDS             complement(5733..6941)
                     /gene="xseA"
                     /locus_tag="VNL92_03785"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWO93863.1"
                     /translation="MSARPRPAMFVYSVGQLVTYLRELLETDTFLADVWVGGEIANLS
                     RSQAGHRYFTLRDSIGSIRCVLFARQAAMVRTALQDGTRVLVHGRVALYEQRGEVQLI
                     VDFVRPEGEGIEQARLERLMAKLEEEGLFDQARKRPLPHFPRRIGVVTSAHGAVFHDI
                     CHVLERRWPLAEVLLADTPVQGDAALAGVPEAIRRFNALPDIDVVIIARGGGGAEELA
                     PFNDEVVVRAIYGSVAPTISAIGHETNTTIADLVADVRAPTPSAAAELAAPDRRQVLS
                     ATEAAAMSLTGAMSRAIVERNGHVEALAFRLPALLPPLEKLRWSLEDAERAMERAALS
                     RCAALGERVAGQQMALAALSPLATLARGYAIVQRADDRRLVRSAKDAPPGTMLDVRLA
                     EGAIRAETVP"
     gene            complement(7011..7571)
                     /gene="efp"
                     /locus_tag="VNL92_03790"
     CDS             complement(7011..7571)
                     /gene="efp"
                     /locus_tag="VNL92_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWO93864.1"
                     /translation="MISTGELKKGATIEIDGTLYQILEYSHIKMGRGSAQVRMKLKDV
                     RAGHTIEKTVQAGEKFKTARVERHPVQYLYEQDGLHYFMNTETFDQLALSEDQVGEAR
                     QYLRENDTCDVLLYENQPIGLELPISVVMEVVETEPGFKGDTQSGGTKPARTDTGLVV
                     NVPLFVNSGDRIKVDTRTGQYQERVS"
     gene            complement(7568..8692)
                     /locus_tag="VNL92_03795"
     CDS             complement(7568..8692)
                     /locus_tag="VNL92_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWO93865.1"
                     /translation="MTTISAPSSTARRLERLRAAIAEHGLDAVLISNTENRRYLSGFS
                     GSAGVLVITASHAVIATDFRYYEQAEQQAPEFRLHKTVGGLQTWFRDLARDLELGGKK
                     LGFEAADLTFQAYQQHVKIIQELPEPERPKLVATTSLVESLRVIKDPEELQLLQRVID
                     IGDAAMAYVQRAIEPGWTEKRVAWEIEKHIREHGGDSLSFPTIVAAGPHSALAHAQPR
                     DRAIEEGEMVVIDMGARLDGYCSDLTRTIFLGEPDDKFLRIYDIVAVAQRTAIETIEA
                     GMTGEQAHMQAHAVIEEAGYGDAFGHGLGHGIGLQVHENPRLARTSGDILREGMVFSI
                     EPGIYLSGWGGVRIEDLATLENGKVRLLSHAPYWNERGTA"
     gene            complement(8689..9132)
                     /gene="aroQ"
                     /locus_tag="VNL92_03800"
     CDS             complement(8689..9132)
                     /gene="aroQ"
                     /locus_tag="VNL92_03800"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWO93866.1"
                     /translation="MRILILNGPNLNTLGAREPEHYGAVTLPEIERRVAARAAELGAS
                     VRFVQSNHEGVLIDELQSAQAEVDGLIINPGGLTHSSVALRDAVVAAGLPAIEVHLSN
                     IYAREDFRHTSLVAPVARGQITGLGWRGYIGALEALVGILEEEQA"
     gene            complement(9183..>10188)
                     /locus_tag="VNL92_03805"
     CDS             complement(9183..>10188)
                     /locus_tag="VNL92_03805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWO93867.1"
                     /translation="AASEIQRRFEERAGLQAPPQPVEIVALGTTLPARCSLAEIESTG
                     DWAAPWRDTATIVQGADLAIANLEAVPSDLAEPYRCQETFSFLTPAAMAQGYAFAGLD
                     VVSLANNHSRDFGGDVLLDGIANLRAAGVQVVGAGANAEEASAPLFVDVGGVRFAFLA
                     YDNVVGEPYAASHGSPGTSAFDETRVVADIRAVRSQADAVVVMLQWGLPEYYQDANAA
                     QRAIAAAAVEAGADYVYGDGPHVPQGLEFRDGAFVHYSLGNFIFDQDWCEWTQVGSIL
                     RLTFHGGRPVAFRLVPYRIADRHRPVPLSGADADAVVALIADATRQPSDAPARLLCEG
                     "
BASE COUNT         1773 a         3435 c         3329 g         1651 t
ORIGIN      
        1 tgaggccgaa atcggcgagg aggccgaagt acgtaatgta ggtaatcagg aagatgtatt
       61 cgccgaaatc gctgacgccg agcgcccgcg ccaggaggat tgtatagccg tacgagagcc
      121 cgcgcgtcag ggcagtcgac atcagcatcc acaccgtgtt gcgcatcacg gtgcgaatgc
      181 ggtcggtcat cgcggggaag gcgagcacac gttgcatgcg cgaatgatag cgcacgccaa
      241 tggaacgaac ccgcagacgc cgtcggcggc gggttctagg gagctatgcg aagctagcgc
      301 tcatctccgc gcgcggagga cacgacgggc tcggcgatcc cagaacgacc ggccccgctt
      361 gcgctccttc cgattcgagg cggcgtcgat ggccgcgtcg agcgagagcg cgcccggacc
      421 ggtgaagaac aacgtgccgg ccgccgatcc gagcagcaca ggaaactcgt acccgccggg
      481 cccgtaaaat cccgtctgcc aatgggcgac gcgcgccgca acagccatgt tgaacgcggc
      541 catcggcgcg aagaagcggg tcatcagccc aagaatcaag gcgaggccac cgaagagctc
      601 ggccatgccc gaaaggtacg ccgagacacg tggcatcggc acattcatct gtcgcagggc
      661 ctcggcgaag ccggagggac tcccgtgctc gtacgcttgc ggatagttct gcccaaaggc
      721 ccgcacgagc accttcggtg gctgcttttc aggcccaccg aacagcttcg gaaaaccgtg
      781 gacgacgagg gttacgccgg tgatgagcct cagcagaaga agtcctacgt cctcgagcgg
      841 cgcaatcttc cgagccagca ttccgacctc cagccccatg cttaccgaca gcataccagc
      901 gagcgcggga caacgtacac cgctcgcacc cggcgcgccg agcccctgtg atcgccggtt
      961 cctatcgtct ggccgagggg tttgttatca tcttgggcga tacccctgac ggacggccgt
     1021 ggagggctcg tgatcaagat cgatatcggc tgcggaatgt cgaagcatct cggcacccta
     1081 ggcgtcgaca tcgccccgct cgccggagtc gatgttgtgg ctgatatcaa caccggcctg
     1141 ccttttcgcg acagcgtcat cgacgaagtc cacatgtcgc acacgctaga gcatgtcgac
     1201 gacttcctgg ccacgatgga agagatctgg cgggtctgca aggacggcgc gttgttctac
     1261 attcgcctcc cccacggttc gtcgtcgttc acgacgtggg tggacccgac gcacaagcgg
     1321 ggcttcaaca tccagacgtt cacgtacttc gacccggagg tgaaccacct ctcctactac
     1381 tcgaaggccc gcttcaagct ggtctattcg aagctcgccc tcagcaccct gccggcggag
     1441 ctgaacggca ctcgcctgcc ccgccgcgtt ctctcgatgg tgctcgaatc ggtagcgaac
     1501 aagagcccgc actggcgcgc tcgcacagaa cgctggttcg cccagctcat cggcttcgag
     1561 gaggcgcgag tcgtcctccg tgcaatgaag cccggcgctc ccgcgcgtcg ggaaggccct
     1621 ggattggcct cggcttcgag gcgaaacgga gcataacttg gaggacggaa cgaaacctca
     1681 gccgccacaa ctgcggaacg tgctcgtgac cggcggcgcc ggctacatcg gcgccgtgct
     1741 cgtaccgaag ctcgtcgccc gtggctacaa cgtccgcgtg ctcgaccgct tcttttgggg
     1801 cgaagcggcc gtgcgcgacg tgctgccaga gaaggcgctc atcaatcggg acatacgcga
     1861 gatcgagccg agcgatttgc gcggcatcga cgccgtcgtg cacctcgcag gcctatcgaa
     1921 cgacccgacc gccgaattcg accctatcgc gaaccacgag atgaacgccg aagcctcgga
     1981 gcgcctggcc cgggcctgcc gcgagagcgg cgtgcgccgc atgaccttcg gatcgagttg
     2041 ctcgatttac gacggcctgc cgccggggcc catctacgat gagaccgccc cagtgcagcc
     2101 gaagggcgcc tacgccatgg ccaagcgata cgccgaagag cgcatcctcg agatctccga
     2161 tcctgaggcg acggacaatg cgggcttctc cgccgtcgtc ctgcgtcagg cgacggtcta
     2221 tgggtacagc cccaggatgc gcttcgacct cgtcgtcaac accttcctga aggacgcact
     2281 cacgcagggg aagctcttcc tccacggcgg cggttggatg tggcgacccc tggtcgacgt
     2341 ggccgatgtt gcggaggccc atatcgtcgc gctcgagtcg cccgcccgaa ccgtggccgg
     2401 cgaggtcttc aacgtcgtcc actcgaacta ccagatccgt gagctcgcca tgatcgtcgc
     2461 gggctcggtt caactggtgg cggggcggca ggttgagctg gaggaagcgc cgatgccgcg
     2521 cttccgccgc gactatcgct gctccaacgc gaagctcgcg accgcgacgg gcttcctgcc
     2581 ggaggttggc gtgctcgagt cggtccagac gatgctggaa aggctgcgtg ggatcgatgc
     2641 gcgcgacttc agtcatccgc gctactacaa catcgagtgg atgacgctgc tcaccgagat
     2701 gcacgagcat ctgcgcggct acgcgcgcgt cctctagcat gcgtgtcctg gttctcggcg
     2761 gccacggaca actggccagc gacatcactc gcctctggac caaccttcaa cccatcccgc
     2821 tgggccacca ggagtgcgac gtgaccgacc gtgcctccat cgacggggcg atcgcggccc
     2881 accgtgcgga cctcgtgctc aatacggccg cgtaccacaa ggtcgacgac tgcgaaggcg
     2941 aaggggcgtt ggcggcgttc gcggtcaacg tcctgggcgc caagaacgtc gccgacgcct
     3001 gccgcgaggc ggatctcccg tacgcatggc tcagcaccga ctacgtcttc agcgggtcga
     3061 agggcgcccc ctaccgcgag ggcgatgcac cggacccggt gagcatgtac ggcgtctcca
     3121 aggccgccgg cgagcacgcg gtgcggtacg ccaactcgcg acattacatc atccgaacga
     3181 gcggcctcta tggcatcgcc ggcgcgagcg gcaagggagg caacttcgtt gagacgatgt
     3241 tgcgtctggc gagggagcgc aagcccatcc gcgtcgtcaa cgaccagcgt ctcgcaccga
     3301 cgagcacggc cgacctcgcc atcgcgctcg agcgactcct cgtcaccgag gcctacggca
     3361 cgtatcacat cacgaatgcc ggcgagtgca gctggtacga gttcgcgggc gccatcttcg
     3421 cgttcacgca ggcggaggtc gacttcgaac cgacgacgtc gaacgagttc ggtgcggccg
     3481 cgcgccgtcc cgcctactct gtcctcgcca acgagcgact cacatcgctg ggcatcgagc
     3541 agccgcggcc ctggcgcgag gcgctcgagg cgtaccttgg ggagaagggc tacctgcgct
     3601 gattgtcgcg cgccgtcaac ggaagaatcg cacccgccgt accgtatact gctcgatgat
     3661 gtagtcgggc tgctggacga ccggcagtcg acccctggaa ggaccttgcc gaacccggcg
     3721 gcccacggcg cgccgcatcg ccacccgcgc catcatcggg atagccttcg cgagggcgaa
     3781 gcggagcgcc aggccacctc cggcgagcac catgccctgc acgatgccgg gcataggccc
     3841 gcgccacggc acaacggccc gagattcggt cgccggcacc gggcgcgcgt cctcctggag
     3901 gggggtgccg caggcgctgc agaatcgatc ggtctcgtca tgcggcgcct gacaacggga
     3961 acagctcacg atcaacggca cggacgacct cctcgctcga tgctcggccg atcatagcat
     4021 gggatgcacg ccggcaagcg cagcctccac ccgttccgtc aggtcgagcg gcggcgcatc
     4081 ctcgcctttg gcatcgacaa cacggaatgc ttgcacgcgc cgctcgcctt gcacgaacgc
     4141 gcgcagcgtt tcgacgacca gcggcccttc gcgggccatg ccccgctctc gtattgcgcg
     4201 gaacaacgcc gtctcgtcga gcgtagctcc gcgacggggc gccgcctcgg cccagtgggg
     4261 cgcgagcgca gcgtcgtcca gggagaaggt gcagtaggtg accggcggac ctgcatccac
     4321 ctctggggtg gcgaggaaca ccgtcacgcc ggagcgccgg gcgcgagtct caatgagctc
     4381 ccggatgacc tgctgccatg tgccggcagg cccgccggga gcggcgggat gaaggttcag
     4441 cagcggcagg cgaaggcacg catcttccgt gaagatgagc atgtagccgg ccagcatcgc
     4501 cacgtcgatc gaaaacggcg cgacgaggcg cagcagcgcg tcatcgaact cacgccgcca
     4561 cgcagggagg ggcgcggttg ggcgcgatcg ttcgccttcg cgctccttac gaaagcggac
     4621 cgaggacaac gtgagcagcg ggatatcgcg cgagcgaacg aggtcgaaga atcgatccgt
     4681 ggcctcggac tcgcccggct cccgattgca gaagacgaag gcgattctcg catcgaggct
     4741 gccggagtcg atggcgcgca aggtcgtttc cagcaagttg tacgagccgg ctcctcgcgc
     4801 tgttgcgaac catccgacct ggagcgtcat cgggacgtat cggccgtgtg tgggcgctcc
     4861 ggcaaggtcc gtccccagcc ggcccttcgc cgcagcttct cgaaggagct catcgcgtag
     4921 acccagcgcg gggctcgcct tccaacgatg gtcgccgagc aaagcgagtc gaggaattcc
     4981 gccggagtct cgaaatccgg catcacaacg tacgtccggc cgacctcgga ggcagaatgg
     5041 gcatcgcttc cgcccgccat ggtgatcccg tactggcgag cgaagcgctc cgcgtgctcg
     5101 ttgtcgcggg ccaggcggat gcgtccattg aacacctcaa tgacgtcgat ctcgggcagg
     5161 atgcgcagca tcgcgtcctc gcccaagcca tcgcgaaagc gatcgaacgg gtgcggtatg
     5221 gctacgacgc ccccttgctc ccgaatgcgc atcacggtct cctcggccga gaggccgggc
     5281 gggacggttt cgcggagaaa gaggccgatg atctcgcctt cacgcgagcg cacctcctcg
     5341 ccgatgacga cgcgaaacgg cgcgatgtcg cgcacgtaca gcgcgccgtc gatggtgtca
     5401 tgctccgtga ccgcgacgca atcgaggcca gccgccacgc agcggcgcac gaactcatcc
     5461 ggcgttatcg cggaatccgg cgaaaagatc gtgtgcgtgt gcaggtcgaa gcggatcgac
     5521 gtcatgctat ccgtccgaat cgagctgctc gcgcagtcgc gtgacccgca gctccgcctg
     5581 cgtcaacagg tcccggcagc gcgcgacgag ggccatgccg gtctcgtagg cctcgatcgc
     5641 ttcctccagt ggaaggctac cgtcctcgag ccgccgaacc gtctcctgaa ggcgttcgta
     5701 cacctgctcg aatcgctctt cgtctttgga gctcatggta ccgtctcggc tcggatcgcg
     5761 ccctcagcca ggcgcacgtc gagcatggtg cctggtggcg catccttcgc actgcgcacg
     5821 agacggcggt catctgcccg ttgcacgatg gcgtagcccc gagcgagcgt ggcgaggggc
     5881 gaaagggccg cgagcgccat ctgctggcct gcgacgcgct cgcccagtgc ggcgcaacgc
     5941 gaaagcgctg ctcgttccat ggcacgctcg gcatcctcca gcgaccaccg gagcttctcg
     6001 aggggtggca acaaggctgg gaggcggaaa gcgagcgcct cgacgtgccc attgcgctcc
     6061 acgatagcgc ggctcatggc gccggtcagg ctcatcgccg ccgcctccgt cgcgctgagg
     6121 acctggcgcc ggtccggcgc ggccagctca gcagcagccg aaggcgtggg ggcgcggaca
     6181 tccgcgacga ggtcggcgat cgtcgtgttc gtttcgtggc cgatcgccga gatggtcggc
     6241 gcgacactgc cgtagatagc gcgcacgacg acctcgtcgt tgaacggcgc gagctcctcc
     6301 gcacctcctc cacccctcgc gatgatgacg acgtcgatat ccggcaaggc gttgaatcgc
     6361 cggatggcct cggggacgcc cgcgagcgcg gcgtcgccct gcacgggcgt atcggcaagg
     6421 aggacctcgg ccagcggcca gcggcgttcg aggacatggc atatgtcgtg gaacacggcc
     6481 ccgtgcgccg acgtcacgac gccgatgcgc cgagggaagt ggggcagcgg acgcttgcgc
     6541 gcctgatcga acaggccttc ctcttcgagc ttggccatga ggcgctcgag gcgcgcctgc
     6601 tcgatgcctt cgccctcggg acggacaaag tcgacgatga gctgtacctc gccacgctgc
     6661 tcgtagaggg cgacacggcc atgcaccagc acgcgcgtgc cgtcctgaag cgcggtgcgt
     6721 accatggcgg cctggcgcgc gaacaggaca catcggatcg acccgatgga atcgcgcagc
     6781 gtgaagtagc gatgcccggc ctgcgaacgc gagagattcg cgatctcgcc gccaacccac
     6841 acgtcggcca gaaacgtgtc cgtctccagc agctctcgca ggtacgtgac gagctgcccg
     6901 acggagtaga cgaacatcgc cggtcgggga cgcgcgctca tcggatcaag gcctcgtgca
     6961 tggcaccggt atgcgtctga tgtcggctgc ctccacgccc caggacgagc ctaggagacg
     7021 cgctcctggt actggcccgt gcgcgtatcg accttgattc ggtccccgct gttgacgaag
     7081 agcggcacgt tgactacaag cccggtatca gtgcgggcgg gcttcgttcc gccgctctgc
     7141 gtatccccct tgaacccggg ctctgtctcg acgacctcca tcacgacgga gattggcagt
     7201 tccaagccga taggctggtt ctcgtacagg agcacatcgc aggtgtcgtt ctctcgcagg
     7261 tattgccgcg cctcgccgac ctgatcctcg gacagcgcaa gctgatcgaa cgtctccgtg
     7321 ttcatgaagt agtggaggcc gtcctgctcg tacaggtact gcaccggatg gcgctcgaca
     7381 cgcgccgtct tgaacttttc gcccgcctgg accgtctttt cgatggtgtg accggctcgc
     7441 acgtccttca gcttcatccg cacctgggcg ctgccgcggc ccatcttgat gtggctgtat
     7501 tcgagtatct ggtagagagt gccatcgatc tcgatggtcg ctcccttctt cagttcaccg
     7561 gtgctgatca agcagttccc ctttcattcc agtacggcgc gtggctcagc agccgcacct
     7621 tcccgttctc aagagtggcc aagtcctcga tacgcacgcc gccccaacca ctcaggtaga
     7681 tgccgggctc gatcgagaac accatccctt cgcggaggat gtcgccggaa gtgcgcgcga
     7741 gccgcggatt ctcgtggacc tggaggccaa tgccgtgccc caagccgtgg ccgaaggcat
     7801 cgccgtagcc cgcctcctcg atgacggcgt gcgcttgcat gtgcgcctgc tcgccggtca
     7861 tcccggcctc gatcgtctcg atcgccgtcc tctgggcgac cgcaacgata tcgtagatgc
     7921 ggaggaactt gtcgtccggc tcgcccagga agatggttcg tgtgaggtcg gagcagtagc
     7981 catcgagccg tgcacccatg tcgatgacga ccatctcccc ttcctcgatc gcgcggtccc
     8041 gaggttgggc gtgtgcgagc gcgctgtgcg gtcccgccgc gacgatagtc gggaacgaaa
     8101 gcgagtcgcc gccgtgctcc cgaatgtgct tttcgatctc ccaagcgacc cgcttctcgg
     8161 tccatccggg ctcgatggcc cgctgcacgt acgccatcgc cgcgtcgccg atatcgatga
     8221 cgcgctgcag aagctggagc tcctctggat ccttgatgac gcgcaggctc tcgaccaagc
     8281 tcgtcgtggc gacgagcttg ggtcgctccg gctcggggag ctcctggatg atcttcacgt
     8341 gttgctgata cgcctgaaac gtgagatccg cggcctcgaa gccgagcttc tttccgccca
     8401 gctccaagtc ccgcgcgagg tcgcggaacc aggtctgaag cccgccgacc gtcttgtgca
     8461 agcggaactc cggggcctgc tgctccgcct gctcgtagta gcggaagtcc gtggcgatga
     8521 cggcgtggga tgccgtgatg accaacacgc cggctgagcc cgagaagccc gaaaggtagc
     8581 gacggttctc cgtgttgctg atgagtacgg cgtcgagccc atgctcggcg atcgccgcgc
     8641 ggaggcgctc gaggcgtcgc gccgtcgatg aaggcgccga aatcgtcgtc atgcttgttc
     8701 ctcctctagg atgcccacca acgcttcgag cgcgccgatg tagcctcgcc aaccgaggcc
     8761 cgtaatctgt ccccgagcga ccggcgccac cagcgacgtg tgacgaaagt cctcgcgggc
     8821 gtagatgttc gagaggtgca cctcgatcgc cgggaggccg gccgcaacga cggcatcgcg
     8881 caatgccacg ctcgaatgcg tgagcccgcc ggggttgata atcagcccat cgacctccgc
     8941 ctgggcgctc tgcagctcgt cgatgagcac cccttcgtga ttcgactgga cgaagcgtac
     9001 gctggcgccg agttcggccg cgcgcgccgc gactcgtcgc tcgatctccg gcagcgtcac
     9061 ggcaccgtag tgttcgggct cgcgggcgcc gagtgtgttg aggttcggtc cattcaggat
     9121 caggatgcgt atggccgaca cactcgcatc atagcagcgc ggcacccctg ccgcacgtca
     9181 ggtcagccct cgcaaagcag gcgagcggga gcatcggacg gctggcgcgt ggcatccgcg
     9241 atcagggcga cgacggcgtc cgcatcagcg ccggacaagg gcacgggccg gtgccggtcg
     9301 gcgatgcgat acggaacgag gcgaaaggcg acggggcgcc cgccgtggaa ggtcagccgc
     9361 aggatcgagc cgacctgcgt ccactcgcac cagtcctgat cgaagatgaa gttgccgagc
     9421 gagtaatgaa cgaaggcgcc atcgcgaaac tcgagccctt ggggaacgtg cggaccatcg
     9481 ccgtacacgt aatcggcgcc ggcctcgaca gcggccgcgg cgatggcccg ctgcgccgcg
     9541 ttcgcgtcct ggtagtactc gggcaagccc cactgcagca tgacgacgac ggcatcggcc
     9601 tgcgagcgca cggcgcggat atcggccacc acacgcgtct cgtcgaaggc actggtgccc
     9661 gggctcccgt gcgacgctgc atagggctcg ccgacgacgt tgtcgtaggc gagaaaggcg
     9721 aagcgtacgc ctccgacgtc gacgaagagg ggtgcggacg cctcctccgc gttcgctccc
     9781 gcgcccacga cctgcacgcc cgctgcccgg aggttcgcta tgccatcgag cagcacgtcg
     9841 cccccgaagt cgcggctgtg attgttggcg agcgacacca cgtcgagtcc agcgaaggcg
     9901 tatccctgcg ccatggcagc cggggtcagg aagctgaacg tctcctggca gcgatatggc
     9961 tctgcgagat cgctcggcac ggcctcgagg ttggcgatgg cgaggtcggc cccttgcacg
    10021 atagtcgcgg tgtcccgcca cggcgccgcc caatcgcctg tcgactcgat ttcggccagg
    10081 gagcagcggg ccggcaacgt cgtgcccaag gcgacgatct ccacgggctg cggcggcgcc
    10141 tggaggccgg cacgctcttc gaagcggcgc tggatctcgg aggcggcg
//
LOCUS       DATMBB010000181        10035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_556999, whole genome shotgun sequence.
ACCESSION   DATMBB010000181 DATMBB010000000
VERSION     DATMBB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556999"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VNL92_03810"
     CDS             complement(<1..786)
                     /locus_tag="VNL92_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWO93868.1"
                     /translation="MQAIDYAAPTTLDEAIAVKRDRRERARVLSGGTDIIVQVREGRR
                     PQVDMLLDVKRIAETTRIELSDEGLTVGAAVPCYQIYENAEIARRWPALVDSASLIGG
                     TQIQSRASLGGNLCNASPAADSIPSLIALGAQAQIAGANGRRSIPVEEFCVGPGQSAL
                     ADDEILVSLFFPAQPPRSGSRFLRFIPRNEMDIAVANAAVALTLDATGTVSAARVAIG
                     AVAPTPLLVPEAGAALVGQPPTAEAIERAVQAAVGAARPITDMR"
     gene            961..1557
                     /locus_tag="VNL92_03815"
     CDS             961..1557
                     /locus_tag="VNL92_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO93869.1"
                     /translation="MSEQATGVRPPIEQAVAPYLNKWGPYRLTRDEVNTTAIRYFCEV
                     AEDANPVYWDDEFARKTRFGRLIAPPQALFSMTFAPWWQPDYVAQANEEDAHRLSAGS
                     AEVEGFEVHRITDEYGYTVNTVTNTESEYLDAFGPGDGRIKTRSMTTSVSPEKQTRVG
                     KGVFITSVTEYRTEVGDRLIGRSTMVLLKYNPAEPRKD"
     gene            1568..1996
                     /locus_tag="VNL92_03820"
     CDS             1568..1996
                     /locus_tag="VNL92_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWO93870.1"
                     /translation="MVTRESLRWAQVNEGDQVTRITFPLSLRNLMIDVAGTRDLYPIH
                     HDRDFARANGARDIFVNTMWYQAFFGRLVTDWAGPEAFLRKLTFQMRAPNCPGDTVTS
                     RATVVRKYEQDGMRLVDIDARLDNQLESDTTIAAMTVELP"
     gene            complement(2010..2459)
                     /locus_tag="VNL92_03825"
     CDS             complement(2010..2459)
                     /locus_tag="VNL92_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HWO93871.1"
                     /translation="MTRRRVVAVIGAGDCDEPTAHLAEEVGRLLARRKTVVVTGGLGG
                     VMEATSRGAAAEGGLVVGIVPTASPSDANAYVDIAIATGMGEARNAIIAHTAEALIVV
                     AGGWGTLSEIAFALRAGKRVVTLGAWSEIAGVERVGSPGEAVDRALA"
     gene            complement(2694..3893)
                     /locus_tag="VNL92_03830"
     CDS             complement(2694..3893)
                     /locus_tag="VNL92_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93872.1"
                     /translation="MIRTTTGAAVATRVACPACGTAPMQPFFRQGAVPVFCNVLHATA
                     DSARRAPRADIALGFCRGCGMVGNVAFEPQLVEYAPGYENSQLFSPAFRAYAESMARD
                     LVERHDLDGRTVVDIGCGQGEFLSLLCEAGAAYGLGFDPSFRGTPESPRVAVVPHAFT
                     AESLPATTGLVCCRHVLEHVADPLGFLRSLRRALDGAPDTPIFFEVPNGLSTLRDLAV
                     WDVIYEHCSYFTAPSLRRLFERSGFAVEGVWEAFGGQMLCLEARPSNIVNLAVTEPAE
                     VETLAPLVEAFAAAQNAEVGRWSRKLRDLRDEGRSVVVWGAGSKGVTFVNVLEPGLVM
                     ALVDVSPRKRGRYVACVGLPVVGPDDLRSLQPDVAIVMNPQYASEVSAVLTELGLSTE
                     VVPVAPC"
     gene            complement(3890..5068)
                     /locus_tag="VNL92_03835"
     CDS             complement(3890..5068)
                     /locus_tag="VNL92_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO93873.1"
                     /translation="MTNSTPICPACSGGDATIFYEVRDVPVHSCVLVRSRQDALDFPR
                     GNLRLASCKGFGFIWNVDFDPTLLDYRQDYEETQGFSPTFNAFARQLAHDLVDRHDLR
                     GKDVLEIGCGKGEFLALLAELGGNRGLGIDPSFAASRRPGTDASLTFIEDYYSEAYTH
                     LTGDLIACRHTLEHVPNVADFVELTRTSAERRPGSIVFFEVPDTLRVLREAAFWDIYH
                     EHCSYFTPGSLARLFRRSAFTVTDVALAYGDQYILLEATLDSAPAEVLPSEDGVGEME
                     ALVDCARRALPANVDAWRTRLQRWTSEGKATVLWGAGSKAVAFLTTTGAGEHVAAAID
                     INPHKHDTFLPVTAHAVLSPERLCTLQPEVVIVMNRVYEGEVRARLAEMGLHPEVVAL
                     "
     gene            complement(5079..6314)
                     /locus_tag="VNL92_03840"
     CDS             complement(5079..6314)
                     /locus_tag="VNL92_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93874.1"
                     /translation="MELYDVITGCRSCGSPRLEPILSLGTTPLADGLIDAEALERPEP
                     RFPLDVAFCEDCSLVQILATVRPEMLFGTDYPYFSSFSPELLRHSRENAESLTRTRNL
                     GPRSLVVEIASNDGYLLRNFVEAGIPVLGIDPAPGPARAAEAAGVRTMCTFFDLDLAE
                     RLAGEGTQADVVIGNNVLAHVPDQNAFIAGIARLLKHDGVAVIEVPYVRDLIERCEFD
                     TIYHEHHCYFSVTALVRLFARHGLSLNHVEHYPIHGGSLRLFVEPREDVGISVRRYLR
                     EEADAGLTHAAFYRDFAARVERTRDSLVQTVTALKAQGRRIAAYGAAAKGATLLNYTG
                     IDSEIIDFVVDRNVHKQGRYMPGVRIPIADPCRLMEDRPDYVLMLAWNFKDEILNQQE
                     AYRALGGRFIVPIPEPVII"
     gene            complement(6337..7380)
                     /locus_tag="VNL92_03845"
     CDS             complement(6337..7380)
                     /locus_tag="VNL92_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014277207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO93875.1"
                     /translation="MRVLITGHDGYIGSVLTPMFVAAGHDVVGLDSYLFEGCALGPES
                     PPNVPAIRKDVRDVEFHDLAGFDAVVHLAGISNDPLGDLNPDCTYDINYHGTVHLAKV
                     AKAAGVQRFLFSSSCSVYGAQGDEFATEEAALNPVTPYGESKILAEEALSLLADDAFS
                     PTYLRNATAYGASPRLRGDLVVNNLVGYALTTGRVFIKSDGSPWRPLIHIEDIARAFL
                     AVLAAPRPVVHDQAFNVGRTEENYRISEVADIVRETVAGSVIVYAEGASPDTRNYRVD
                     CQKFARAVPQFQPQWTVRRGAEQLASAYRQHGLTELAELTGSRFQRIKRVRELQDAGL
                     LDAELRWRVPALT"
     gene            complement(7384..8154)
                     /gene="rfbF"
                     /locus_tag="VNL92_03850"
     CDS             complement(7384..8154)
                     /gene="rfbF"
                     /locus_tag="VNL92_03850"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HWO93876.1"
                     /translation="MKVAILAGGLGSRLAEETEIRPKPMVEVGGKPILWHIMKHYAHF
                     GFRDFAIALGYKGDYIKKYFVEYCSLASDLRVDLATGAIARCSAEPEQWSVELIDTGF
                     GTATGGRIKRLGPHLGGQTFMLTWGDGVSDIDLCDLLAFHRYHGKLATVTAVRPPARF
                     GRLDLEGDRVARFEEKPQLSEGWINGAFFVLEPEVLEYIDGDDTQWEREPLQRLADDG
                     QLMAYRHESFWQCMDTIRDRKLLEELWQRGDAPWKVWR"
     gene            complement(8160..9131)
                     /locus_tag="VNL92_03855"
     CDS             complement(8160..9131)
                     /locus_tag="VNL92_03855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWO93877.1"
                     /translation="MAQAAPKVTIGVPVYNGERFLAEALDSALAQTYGDFEIIISDNA
                     SQDGTDEIARSYVARDARVRFVENQTNVGAARNYNKLVRLARGRYFKWLAADDIMAPE
                     FLARCIDVLERHPDVALAYPRTRCIDEDGLVYPDDPWSPMVWPAEPSARMRLYLEAAP
                     WAPVLVFGLMRTEALRRTRLIGPYPGGDLNFVAELALEGAFVELTPALLFFRLHADSL
                     TWAKADPERHVAFYAPEQRSRLARAFLARRHGLEYGRSVLRSDVGVRQKAALASYLAT
                     RAVAGALRGLRRRVGSHPSDTAASVTPPQTHWTELIEAGDRVVALAR"
     gene            complement(9258..>10035)
                     /locus_tag="VNL92_03860"
     CDS             complement(9258..>10035)
                     /locus_tag="VNL92_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWO93878.1"
                     /translation="GEPGGRPVRPGLSLGDIAAGLFAAIGILAAVHERERSGEGQMLD
                     ISMLDCQIAILENAYMRYFATGKPPEPIGTRHPSATPFQAFPTADGHLVIALSWGVEN
                     QWELLCAELGVPQLMDDERFITSGRRTANHAELEPLLEAAFRARTTAEWLPRLEAIGV
                     PCGPLNTIPQAAEMPQVAAREMLVDVAHPRIGSLRIANSPVRLSRTPGRVHGGPPDMG
                     EHTIEVLRGRLGLTEERIDALKQAGVIATERPPLPDMLRG"
BASE COUNT         1741 a         3242 c         3432 g         1620 t
ORIGIN      
        1 gcgcatatcg gtaatcgggc gcgccgcgcc aacggcagcc tgcaccgcgc gctcgatcgc
       61 ctcggcggtc gggggttggc cgaccagcgc tgcgcctgcc tcgggcacca gcaacggcgt
      121 cggcgccacc gcgccgatgg cgacgcgcgc ggcgcttacg gtgccggtgg catcgagcgt
      181 caaggcaacg gccgcattgg ccacggcaat gtccatctca ttgcgtggga tgaaccggag
      241 gaatcgggat ccggatcgcg gaggttgcgc cggaaagaag agcgacacga ggatctcgtc
      301 gtcggccagg gcggattggc cgggcccaac gcagaactcc tcgaccggaa tcgatcgtcg
      361 cccgttcgcc cccgcgatct gggcttgcgc gccgagggcg atgagcgagg gaatgctatc
      421 ggcggccggc gatgcgttac agaggttccc acccaaggac gccctgctct gaatctgcgt
      481 gccgccaatg agcgaggcgg aatcgacgag ggcgggccac cggcgggcga tctcggcgtt
      541 ttcgtatatc tggtagcacg gcacggccgc gcccaccgtg agtccttcgt cgctgagctc
      601 gatacgcgtc gtctcggcga tccgcttcac gtcgagaagc atgtcgactt gtggccgccg
      661 cccctcgcgc acctgcacga tgatgtccgt cccgccgctg agcacgcggg cacgctcgcg
      721 ccggtcgcgc ttcaccgcga tcgcttcatc gagagtggtt ggcgcggcgt aatcgattgc
      781 ttgcaccggt gatgaacctc ctctcgttag gtccgccgca gcatagcggt ccctccgatt
      841 cccgcgcaat cctatcatag tcgacgggcg gctggtgcga agaggcacaa tggattgaaa
      901 gacggggccg gcacgggtag actccgcacg aaccaatgac aagcaggggg aggatgttgc
      961 atgagcgaac aggcgacggg tgtgaggccg cccatcgagc aagccgtggc cccgtacctc
     1021 aacaagtggg ggccatatcg cttgacccgc gacgaggtga acacgacggc gattcgctac
     1081 ttctgcgagg tggcggagga cgcaaacccc gtctattggg atgacgagtt cgcgagaaag
     1141 acccggtttg gccgtctcat cgcgccgccg caagcgctgt ttagcatgac gttcgccccg
     1201 tggtggcaac ccgactacgt ggcccaggcc aacgaggagg acgcgcacag gctaagcgcc
     1261 ggcagcgccg aggtcgaagg cttcgaagtg caccgcatca cggacgaata cggctatacg
     1321 gtcaatactg tcacgaacac ggagagtgag tacctcgatg cgttcgggcc gggcgacggc
     1381 cgcatcaaga cgcggtccat gacgacgagc gtgtcgccgg agaagcagac ccgagtaggc
     1441 aagggggtct tcattaccag tgtcaccgag tatcgcaccg aggtcggcga tcggctcatc
     1501 ggccggtcga cgatggtgct cctgaagtac aaccccgccg agccgcgaaa ggattaggag
     1561 gagagcgatg gtgacgaggg agagcctccg ctgggctcaa gtgaatgagg gagaccaggt
     1621 gacgcgcatc acctttcccc tcagtctgcg gaacctgatg atcgatgttg cgggcacgcg
     1681 cgacctctat cctatccatc acgaccgcga cttcgcccgg gcgaacggcg cacgcgacat
     1741 cttcgtcaac accatgtggt atcaggcatt cttcggacgg ctcgtcaccg attgggctgg
     1801 gccggaggcc ttcctccgga agctcacctt ccagatgcgg gcgccgaact gccccgggga
     1861 tacggtgaca tcccgcgcga cggtcgtccg gaagtacgag caggacggga tgaggctcgt
     1921 cgatatcgat gcgaggctcg acaaccagct cgagagcgac acgaccatcg ccgcgatgac
     1981 cgtggagttg ccgtagcctg caacgcgacc tacgcgaggg cgcggtcgac ggcttccccg
     2041 ggcgatccga cgcgctcgac gccggcgatc tcggaccaag cgccgagggt gacgacgcgc
     2101 ttgcccgcac gcagggcaaa cgcgatctcg gagagggtgc cccagccgcc ggcgacgacg
     2161 atcaatgctt cggccgtatg cgcgatgatg gcgttgcgtg cttcgcccat gccggtagcg
     2221 atagcgatgt cgacgtacgc attcgcgtcg ctgggcgagg ccgtgggcac gatgccaacg
     2281 acgaggccgc cttccgcggc cgcgccacgc gacgtggctt ccatcacacc cccgagcccg
     2341 cccgtcacta cgacggtctt gcggcgcgca agaagacggc ccacctcttc cgcaaggtgg
     2401 gccgtaggct catcgcagtc gcccgcgccg ataaccgcga cgacgcgtct cctcgtcatc
     2461 gctgactacg cggccactcg agtggcggac ccgcgtcgta tgagagcggc gtcgcgccgg
     2521 tagccgcagt tgaggcacac gaattcgctc gtagcgaggt catcttcgag gaagagtcgt
     2581 ccctcgcatc gagggcagat cagcgtcgac agcatcgtct catcacctcc gcttctcttc
     2641 gtgcgtatca tagcgagact ccatccccat cgtcaactaa catgataacg atattagcac
     2701 ggtgctacag gcacgacctc ggtcgaaagg ccgagctccg tgagcacggc gctcacctcg
     2761 ctcgcgtact gcgggttcat cacaatggcg acatcgggct gcagcgatcg aaggtcgtcc
     2821 ggaccgacga ccggaagtcc gacgcatgcg acgtaacgtc cgcgcttccg agggctcacg
     2881 tcgacgagcg ccatgacgag accgggctcg aggacgttga cgaaggtgac ccccttcgac
     2941 ccggcccccc aaacgacaac gctgcgcccc tcgtcgcgga ggtcacgcag cttgcgcgac
     3001 cagcggccga cctcggcatt ctgcgctgcg gcgaacgcct cgacgagtgg cgcgagggtt
     3061 tcgacttcgg caggctccgt gaccgcgaga ttgacgatgt tggatggccg ggcctcgagg
     3121 cagagcatct ggccgccgaa cgcttcccag actccctcca cggcgaatcc gctgcgctcg
     3181 aacagacggc gcagcgaggg cgcggtgaag taggagcaat gttcgtagat gacgtcccac
     3241 acggccaagt ctcgcagggt ggagagccca ttcgggacct cgaagaagat gggcgtatcg
     3301 ggtgctccat cgagcgccct gcgcagtgag cgcaggaacc ccagcgggtc ggctacgtgc
     3361 tcgaggacgt ggcgacagca cacgagcccc gtcgtcgcgg gcagcgattc ggcggtaaac
     3421 gcgtgcggca caaccgcgac ccgaggcgac tcaggcgtgc cgcggaagct tgggtcgaag
     3481 cccaggccgt acgcggcacc ggcctcgcag agaagcgaga ggaactcgcc ctgtccgcac
     3541 ccgatgtcga ccaccgttcg tccgtccaga tcgtgacgct ccacgaggtc gcgcgccatg
     3601 ctctcggcgt aggcgcggaa agccggcgaa aagagctgcg agttctcgta gccgggggcg
     3661 tactcgacga gttgcggctc gaacgccacg ttacccacca ttccgcagcc gcggcaaaag
     3721 ccgagcgcga tgtcggcgcg tggggcgcgc cgagccgaat ctgccgtcgc atgcagcacg
     3781 ttgcagaaga cggggacggc gccctgtcgg aagaagggct gcatcggcgc ggtgccgcag
     3841 gctgggcaag ccacgcgggt ggcgacggcg gcccccgtgg tggtccggat catagcgcga
     3901 cgacctccgg atggaggccc atctccgcca accgcgctcg gacttcgcct tcgtagaccc
     3961 ggttcatcac gatcacgacc tccggttgca gcgtgcagag acgctccggc gacaggaccg
     4021 cgtgggccgt gacgggcagg aacgtatcgt gcttgtgggg gttgatatcg atggcggcgg
     4081 caacgtgctc gcccgcgccg gtggtcgtca ggaaggcgac ggccttcgat ccggcgcccc
     4141 agagcacggt cgccttgcct tcgctggtcc atcgctgcag gcgcgtgcgc catgcgtcga
     4201 cgtttgccgg caatgcccgg cgcgcgcagt ccacgagcgc ctccatctcg ccgacgccgt
     4261 cctcgctcgg cagcacctcg gccggtgcgg agtcgagcgt ggcctcgagc aggatgtact
     4321 ggtcgccgta ggcgagcgcg acgtcggtga cggtaaaggc gctacgccga aacagtcgcg
     4381 ccagcgatcc gggcgtgaag tacgagcagt gctcgtggta gatgtcccag aaggcggcct
     4441 cgcggagcac gcgaagcgta tccggcactt cgaagaagac gatgctgccc ggccggcgct
     4501 cggcgctcgt tcgcgtcaac tcgacgaagt cggcgacgtt gggaacgtgc tcgagggtgt
     4561 gccggcaggc gatgagatcg cccgtaaggt gcgtgtaggc ctcgctgtag tagtcctcga
     4621 tgaacgtgag gctagcgtcg gttccaggcc tgcgagatgc ggcgaaactc gggtcgatgc
     4681 cgaggccgcg gttccctccc agctcggcca acagcgcgag gaactcgccc ttgccacagc
     4741 cgatctcgag cacgtccttc ccgcgaaggt cgtgccggtc gacgaggtcg tgcgcgagtt
     4801 gcctggcgaa ggcgttgaac gtgggcgaga agccctgcgt ctcctcgtag tcctgacgat
     4861 agtcgagcaa cgtcgggtcg aagtcgacgt tccatatgaa gccgaagcct ttgcaagagg
     4921 cgaggcgcag gttcccccgc gggaagtcga gtgcgtcctg ccgcgagcgg acgagcacgc
     4981 agctgtgcac ggggacgtct cgcacctcgt agaagatcgt tgcgtcgccg ccgctgcagg
     5041 cggggcagat cggcgtggag ttggtcatgg tcctcgcctc atatgatcac gggctcggga
     5101 atcgggacga tgaagcggcc gccgagcgcg cggtaagcct cctgctggtt gaggatctcg
     5161 tccttgaagt tccacgccag catcagcacg tagtccggcc ggtcctccat gaggcggcag
     5221 gggtcggcga tggggatacg cacgccgggc atgtaccgtc cctgcttgtg cacgttccga
     5281 tcaacgacga agtcgatgat ttcgctgtcg atacccgtgt agttcaggag cgtcgctccc
     5341 ttcgctgccg cgccataggc agcgatgcgc cgcccctgcg ccttcagcgc cgtgacggtc
     5401 tgcacgagcg aatcccgggt gcgctccacg cgggcggcga agtcgcgata gaacgcggcg
     5461 tgcgtgagcc cggcgtctgc ttcctcccga aggtagcgcc gcacggaaat gccgacgtcc
     5521 tcacggggct ccacgaacaa gcgcaacgag cccccgtgga tcgggtaatg ctcgacgtga
     5581 ttcagcgaga ggccgtggcg agcgaataac cgaacgagcg cggtgaccga gaagtagcag
     5641 tggtgctcgt ggtagatggt atcgaactcg cacctctcga tgaggtcgcg gacgtagggc
     5701 acctcgatga cggcgacgcc atcgtgcttg agcagccggg cgatgcccgc aatgaaggcg
     5761 ttctggtcgg gcacgtgggc cagcacgttg ttaccgatga cgacgtcggc ttgcgtcccc
     5821 tccccggcca ggcgctcggc caagtcgaga tcgaagaatg tgcacatggt ccgcacgccg
     5881 gccgcttccg ccgcgcgcgc cggtccgggt gccggatcga tgccgagcac gggaatcccc
     5941 gcctcgacga agttgcggag caggtagcca tcgttgctcg cgatctcgac cacgaggctt
     6001 cgagggccaa ggttccgcgt gcgcgtgagc gactctgcgt tctcgcgcga atgtcgcagc
     6061 agctcaggcg agaaggacga gaagtacggg tagtcggtgc cgaacagcat ctcgggacgg
     6121 acggtcgcga ggatctgcac aagcgagcag tcctcgcaga acgcgacatc gagcgggaac
     6181 ctgggctccg gcctctcgag cgcctcggcg tcgatcaatc cgtccgcgag cggcgtcgtt
     6241 ccgagagaca ggatcggctc gagtcgaggc gacccgcagg atcggcaacc ggtgatgaca
     6301 tcgtacaatt ccattcctcc tacgcgtgct gcgaggtcac gtgagcgcgg gcactcgcca
     6361 cctcagctcc gcatccagca ggcccgcgtc ctgcagctcg cggacgcgct tgattcgctg
     6421 gaagcgcgag cccgtgagct cggcaagctc ggtcaggccg tgctgtcggt atgccgaggc
     6481 gagctgctca gcgccgcgcc gcaccgtcca ttgtggctgg aactgcggga cggcccgggc
     6541 gaacttctgg caatcgacgc ggtagttgcg cgtgtcgggg cttgcgccct cggcgtaaac
     6601 gatgacgctt cccgcaaccg tctcacgcac gatatccgcg acttcggaga tgcggtagtt
     6661 ctcttccgtg cggcctacgt tgaaggcttg gtcgtgcacc accggacgcg gcgccgccag
     6721 tacggccagg aaggcgcgag cgatgtcctc gatgtggatc agggggcgcc acggtgagcc
     6781 gtcgctcttg atgaaaaccc ggcccgtcgt gagagcgtag ccaacgaggt tgttgaccac
     6841 caggtcgccg cgaagtcgcg gcgaggcccc gtacgccgtg gcgttccgta ggtaggtggg
     6901 gctgaatgcg tcgtcggcca gcagcgacag cgcttcctcg gcgagaatct tcgactcgcc
     6961 gtagggcgtc actgggttga gtgccgcttc ctccgtcgcg aactcgtcgc cctgcgctcc
     7021 atacacgctg cacgacgagg agaagaggaa gcgttgcacg ccggcagcct tggcgacctt
     7081 ggccaggtgc acggtgccgt ggtagttgat gtcgtaggta cagtccgggt tgaggtcgcc
     7141 gagcgggtcg ttggaaatac cggcgaggtg cacgacggca tcgaagcccg ccaggtcgtg
     7201 gaactcgacg tcacgcacgt ccttgcgaat ggcgggcacg ttcggcgggc tctcgggtcc
     7261 gagcgcgcat ccctcgaaca gatatgaatc gaggccaacg acgtcgtggc ccgcagccac
     7321 gaacatcggc gtcaggaccg acccgatgta gccatcgtgc ccggtgatga ggacacgcat
     7381 ccgctacctc cagactttcc agggcgcgtc gccccgctgc cacagctcct cgaggagctt
     7441 cctgtcacgt atcgtgtcca tgcactgcca gaacgactca tgacgatagg ccatgagctg
     7501 cccgtcgtcg gcgagacgct ggagcggttc ccgctcccac tgcgtgtcgt cgccgtcgat
     7561 gtactcgagc acctcgggct cgaggacgaa gaacgcgccg ttgatccagc cttccgagag
     7621 ctgaggcttc tcctcgaagc gggccacgcg gtcgccttcg aggtccagcc tgccgaagcg
     7681 agccggaggc cggaccgcgg tcacggtcgc gagcttgccg tggtatcgat ggaaggcgag
     7741 gaggtcacac aggtcgatgt cggagacgcc gtcgccccag gtgagcatga aggtttgtcc
     7801 accgaggtgc gggccgagtc gcttgatgcg cccacccgtg gccgttccga accccgtgtc
     7861 gatgagctcc accgaccact gttcgggctc ggccgagcac cgggcgatgg cgcccgtcgc
     7921 caggtcaacc cgcaggtcgc tcgcgagcga gcagtactcc acgaagtact tcttgatgta
     7981 gtcgcccttg tagccgagcg cgatcgcgaa gtcgcggaac ccgaagtggg cgtagtgctt
     8041 catgatgtgc cacaggattg gctttccgcc cacctcgacc atcggcttcg ggcggatctc
     8101 ggtctcttcc gcgagccgag agccgaggcc accggcgaga attgccacct tcatgtctgt
     8161 cacctcgcga gggcgaccac gcggtcgccg gcttcgatga gttcggtcca gtgagtctgg
     8221 ggtggagtca cggaagcggc cgtgtcgctc ggatgcgagc caacgcgccg gcggaggccg
     8281 cgaagagcgc cggctacggc ccgcgtagcc aagtacgacg cgagggccgc cttctgtcgc
     8341 actccgacgt ccgatcgcag gacggaacgc ccatattcca agccatggcg ccgagcgagg
     8401 aaagcgcgcg cgaggcggct ccgctgctcg ggcgcgtaga acgccacgtg ccgctctggg
     8461 tcggccttgg cccatgtgag ggaatcggcg tgcaggcgga agaacaggag cgccggggtc
     8521 agctcgacga atgctccttc gagcgccagc tcggccacga aattgaggtc gccccccgga
     8581 tacggaccga tgaggcgtgt ccggcgtagc gcttccgtgc gcatgaggcc gaacacgagc
     8641 accggcgccc agggcgccgc ttccaagtag agacgcatac gagccgatgg ctccgccggc
     8701 cagaccatcg gcgaccacgg gtcgtcgggg tatacgaggc cgtcctcgtc gatgcatctg
     8761 gtccgcgggt acgcgagggc cacatcgggg tggcgctcga gcacgtcgat gcagcgcgcg
     8821 aggaactcgg gcgccatgat gtcgtcggcg gcgagccact tgaagtagcg gccccgagcg
     8881 aggcggacga gtttgttata gtttcgtgct gccccgacgt tggtttggtt ctcgacgaat
     8941 cggacgcgtg catcgcgagc tacgtaggag cgcgcgattt cgtccgtccc gtcctgcgag
     9001 gcgttgtccg agatgatgat ttcgaaatcg ccgtaggtct gcgccagcgc cgaatcgagc
     9061 gcctcagcca gaaagcgctc cccgttgtac acgggtacgc ctatcgtgac cttcggggca
     9121 gcctgtgcca tgcgccgacc tccctgctgg cgtcggagcc agcacgaaca tcgtcggcca
     9181 gtttcgtgca ggagatgttg agcggagggt gccgtaaggt tgcgcgaagg ccgagcgcgt
     9241 cccgccggct ggcgaggtta gccccgaagc atatccggca gcggaggccg ttcggtcgcg
     9301 atgacgccgg cctgcttgag cgcgtcgatg cgttcctccg tcagacccag gcggccccgt
     9361 agcacctcga tcgtgtgctc gcccatgtcc ggaggcccgc cgtgaactcg gcccggcgtc
     9421 cgagatagcc gcacgggaga attcgcgatg cggagggagc caatgcgcgg atgcgcaaca
     9481 tcgaccagca tctcccgggc cgcgacctgg ggcatctcgg ccgcttgtgg gatggtgttc
     9541 aagggtccgc agggcacgcc gatcgcctcg agccgcggca gccactcggc cgttgtccga
     9601 gcgcggaagg ccgcctccag cagcggttcg agctcggcgt ggttcgccgt gcggcgtccg
     9661 ctcgtgatga atcgctcgtc gtccatcagc tgggggacgc ctagctcggc gcagagcagc
     9721 tcccactggt tctccacgcc ccagctcagg gcgatcacga ggtggccgtc cgctgtcggg
     9781 aaggcctgga acggcgtagc ggacgggtga cgtgtaccga tcggctccgg tggcttccca
     9841 gtcgcgaagt aacgcatgta ggcgttctcc aggatggcta tctggcagtc cagcatggag
     9901 atatcgagca tctggccttc accggaccgc tcccgctcgt gaacggcggc caggatgccg
     9961 atggcggcga agagcccggc cgcgatatcg cccagcgaga gtcccgggcg cacgggacgt
    10021 cctcccggct ctccg
//
LOCUS       DATMBB010000182         3411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_321070, whole genome shotgun sequence.
ACCESSION   DATMBB010000182 DATMBB010000000
VERSION     DATMBB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321070"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VNL92_03865"
     CDS             <1..608
                     /locus_tag="VNL92_03865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO93879.1"
                     /translation="GVGEDNMVREIGIVPKPLQKPYPPVYQPFAFSESTIRWCARQGI
                     QPILSPVDISHQTHLYHAYQDEAAKHGRQLAFGEGMGLLRDVLIADTMEEALALYVNG
                     APFVNQNWFAPFGFGQGIEAPGEKREDLTLEKMLERGHLLVGTVDSVTRQLEALMKRA
                     PVYHIMAYQYIGLTPHEAQMKSIELFGSKVLPRFKDVVSMS"
     gene            640..1083
                     /locus_tag="VNL92_03870"
     CDS             640..1083
                     /locus_tag="VNL92_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93880.1"
                     /translation="MRNIYFDVDATLISVQGLHLRPHAREVIARLRAHGLRVYLWSAM
                     GDWYAKNAAIKHGLECDGFFSKPADRDDWTLEPHLPEPHFAFDDCEERFIETFDGLIV
                     RPYYDPDPNDDELLRVLDWIEVHPSVRELIVASDADAHQPRSVPP"
     gene            1156..1428
                     /locus_tag="VNL92_03875"
     CDS             1156..1428
                     /locus_tag="VNL92_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWO93881.1"
                     /translation="MPTYEYRCSGCQAFYERREGFDAPAVHPCERCGAEARRVLRPPA
                     IVFKGAGFYATDSRNGRPADGASEVESVTAGASDDDHGHSHGDDLD"
     gene            1435..1884
                     /gene="dtd"
                     /locus_tag="VNL92_03880"
     CDS             1435..1884
                     /gene="dtd"
                     /locus_tag="VNL92_03880"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWO93882.1"
                     /translation="MRLVIQRVTEAAVTVDDRETGRIGAGLVIFVGVASGDTEDDAIR
                     LARKTAHLRIFPAESGRFDRSLIDICGAALVISQFTLLADVRRGRRPYFGEAAAANEA
                     EPLVRRYADSLRALGIEVAEGVFGAHMVVRLANDGPVTITLASEDLG"
     gene            2021..3322
                     /locus_tag="VNL92_03885"
     CDS             2021..3322
                     /locus_tag="VNL92_03885"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWO93883.1"
                     /translation="MPARVVIGGQWGDEGKGRIVDLLARDAHAIVRYSAGNNAGHTVI
                     NELGEFALHLIPAGIFYSDKVCVIGNGVAVDPAVLIGEIEMLESRGVSTANLVVSDRA
                     HVIMPYHTIIDRLDEELRGAAAVGTTGRGVGPAFVDKVGRLGLRMADLIDPAAFAERL
                     RFVLTYKNRVLTQLYGADPLDFDTLRDQYTAYADKLRPYVRDTSALLRDMLAQGRDVL
                     LEGAQGSLLDLDSGTYDYVTSSVPSSTAAGAAIGAGISPMAIGEIVGVYKAYMTRVGN
                     GPMPTELLDETGDLLRMGGPRPEVGTTTGRPRRCGWFDAVVSRHSAAINGVSMAVLTR
                     LDVLDRLPRVFVCTAYELDGERVTTPPASASALLRAKPVFEELAGWQQSTAEARSFRQ
                     LPRAAQAYVRRIEELLATPIRVISVGPERDQAIFVGERGEP"
BASE COUNT          594 a         1108 c         1095 g          614 t
ORIGIN      
        1 agggtgtagg cgaggataac atggtgaggg agatcggcat cgtgccgaag cccctccaga
       61 agccctaccc gcctgtgtac caaccattcg ccttcagcga atcgacgatt cgctggtgcg
      121 cgcggcaagg catccaaccc atcctctcgc ccgtcgacat ctcgcaccag acccacctct
      181 accacgccta ccaggacgaa gcggcgaagc acggccgcca gctggccttc ggcgaaggca
      241 tgggattgtt gcgtgacgtg ctcatcgccg acacgatgga ggaggcgctc gcgctgtacg
      301 tcaatggggc gcccttcgtc aaccaaaact ggttcgctcc gttcggcttc ggccagggca
      361 tcgaggcgcc cggcgagaag cgcgaggacc tcaccctcga gaagatgctc gaacgcggcc
      421 atctcctcgt ggggaccgtc gactcggtga cgcgccaact cgaggccctc atgaagcgcg
      481 cgcccgtgta tcacatcatg gcgtaccagt acatcgggct cacgcctcac gaggcgcaga
      541 tgaagtccat cgagctcttc ggctccaagg tgttgccgcg attcaaagac gtggtgagca
      601 tgagctgact ccctggatgc ggcgccgggc ggaacaagta tgcgcaatat ctacttcgat
      661 gtggacgcga cgctcatctc ggtgcaggga ttgcacttgc ggccgcacgc gcgcgaagtg
      721 atcgcacgtt tgcgcgcgca cgggctgcgc gtgtaccttt ggtcggctat gggcgattgg
      781 tacgccaaga acgccgccat caagcacggc ctggagtgcg acggattctt cagcaagccg
      841 gccgaccgtg acgactggac gctggagcct catctcccgg agccgcactt cgccttcgac
      901 gactgcgagg agcggttcat cgagacgttc gatggcctca tcgttcggcc ctactacgat
      961 cccgacccta atgacgacga gcttctgcgt gtcctcgact ggatcgaagt tcacccgagc
     1021 gtcagggagc tcatcgttgc gagcgatgcg gacgcgcatc agcctcggtc ggtgccgccg
     1081 tagcgcgccg cttgtcgtct tcgttgtgaa gccctacact tctgctagca tcttcctcgg
     1141 aggcaccgaa aacctatgcc gacctatgaa taccgctgca gcggatgcca ggcgttctac
     1201 gagcggcgcg agggtttcga cgcgccggcg gtgcacccgt gcgagcgctg cggcgccgaa
     1261 gcgcgacgcg ttctgcgccc gcccgcgatc gttttcaagg gcgcaggctt ctacgccacc
     1321 gacagccgta atggccgccc agccgacggg gccagcgagg tcgagagtgt gactgccggc
     1381 gcctcggatg atgaccatgg tcacagccac ggtgacgacc tcgactgaac ggccatgcgg
     1441 ctcgtcatcc agcgggtgac tgaagccgcg gtgacggtag atgatcgtga gaccggccgc
     1501 attggggccg gtctcgtcat attcgtgggc gtcgcgtccg gcgatactga ggacgacgcg
     1561 atccgcctcg ctcggaagac agcgcacctt cgcatcttcc cagccgaatc ggggcgattc
     1621 gaccggtccc ttatcgacat ctgtggcgcg gcgttggtca tctcgcagtt caccctgctc
     1681 gccgatgtac gccgcgggcg ccggccctac tttggggagg ctgctgcggc aaatgaagcc
     1741 gagccgctcg ttcggcgcta cgccgattct cttcgggcgc tcggcatcga ggtggcggag
     1801 ggggtgttcg gtgcccacat ggtcgtacga ctggcaaacg atggtccggt cacgatcaca
     1861 ctcgcctccg aggaccttgg ctagtcgatc cccgtgatcc ccgtatagtg gcgatcgcgc
     1921 tcgagaggcc tctctgctat actccgcgtc gggatgcccc ccagccctgg ggggcgcttg
     1981 tgcgtgtgct tggcacgcac cctgcattaa ggagaaggac atgccggcgc gagtcgtcat
     2041 cggtgggcag tggggcgatg aaggcaaggg gcgtatcgtt gacctgctgg cgcgcgacgc
     2101 acacgccatc gtccgctact cagcgggcaa caacgccggc cacaccgtca tcaacgagct
     2161 cggggagttc gcactgcatc tcatccccgc cggaatcttc tacagcgaca aggtttgcgt
     2221 catcggcaac ggggttgccg tggaccctgc cgtcctcatc ggcgagattg agatgctcga
     2281 atcccgcggc gtgagcacag cgaacctggt cgtctccgac cgcgcgcacg tcatcatgcc
     2341 gtaccacacg attatcgatc ggctcgatga agaactccgc ggcgcggccg ccgttggcac
     2401 cacagggcgt ggcgttggac cggcgttcgt cgacaaggtg ggacgcctcg gcctgcgcat
     2461 ggccgatctc atcgacccgg cggcgttcgc ggagcgcctg cggttcgtgc tgacctacaa
     2521 gaatcgtgtc ctcacccagc tctatggcgc cgacccgctc gacttcgata cactccgcga
     2581 ccaatacacg gcgtacgcgg acaagctgcg accttacgtg cgggatacgt cggcgctact
     2641 ccgcgacatg ctcgcacaag gacgcgatgt gctgttggaa ggtgcacagg gcagcctcct
     2701 cgatctcgat agcggaacgt acgattacgt gacatcctcc gtaccctcct cgactgcggc
     2761 cggcgccgcc atcggtgcgg gtattagtcc gatggccatt ggcgagatcg tgggcgtata
     2821 caaggcgtac atgacccgag taggcaacgg gccgatgcca accgagctcc tcgacgaaac
     2881 aggagatttg cttcgcatgg gggggcctcg cccggaagtg gggacgacca cgggacgtcc
     2941 gcgccgctgc ggctggttcg atgccgtcgt ctcgcgacat tccgcggcca tcaacggcgt
     3001 cagcatggcc gtgctcacgc gcctcgacgt gctcgaccgc cttccgcgcg tgttcgtatg
     3061 cacggcctac gaactggatg gcgagcgcgt gacaacgccg cccgccagcg cgagcgccct
     3121 cctacgcgca aagcccgtct tcgaggagtt ggccggctgg cagcaaagca ccgcggaagc
     3181 gcggagcttt cgacagttgc cgcgggctgc gcaggcgtac gttcggcgga tcgaggaact
     3241 gttagcgacg ccgatccgag tcatctccgt cgggccggag cgcgatcaag cgattttcgt
     3301 gggtgaacgc ggggagccgt aaaggggatg ccatgggcga agccgtaacc ttctggatcc
     3361 acctcttggg catggccgtt ttcctcgggt cccagatcat gcttgccttc g
//
LOCUS       DATMBB010000183        15040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_160191, whole genome shotgun sequence.
ACCESSION   DATMBB010000183 DATMBB010000000
VERSION     DATMBB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160191"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VNL92_03890"
     CDS             <1..887
                     /locus_tag="VNL92_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWO93884.1"
                     /translation="VAAPEGPMPQTREHILLARQVDVPNIVVFLNKVDMMDDPELLDL
                     VELELRELLTNYEFPGDEVPIIRGSALQALESTSTDPGAPEYAPIWELMKAVDEYIPT
                     PERPRDQPFLMPIEDVFGIKGRGTVVTGRIERGVVKVGEEVEIVGLRETRKTTVTGVE
                     MFKKLLDTGEAGDNVGCLLRGIERDDVERGQVLAKPGTITPHTKFEGEVYVLSKEEGG
                     RHTPFFTGYRPQFYIRTTDVTGSIALPEGVEMVMPGDNVKMTIELIQPIAIEDGLRFA
                     IREGGRTVGAGVVTKVLQ"
     gene            890..1216
                     /gene="rpsJ"
                     /locus_tag="VNL92_03895"
     CDS             890..1216
                     /gene="rpsJ"
                     /locus_tag="VNL92_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWO93885.1"
                     /translation="MARGDTATRRKIRIRLKAYDHRILDQSAVQIVEAAERTGAAVAG
                     PVPLPTHIKKFTVNRSPFIDKDSREQFEIRTHKRLIDILDPTSRTVDTLMRLQLPSGV
                     DIEIKL"
     gene            1216..1842
                     /gene="rplC"
                     /locus_tag="VNL92_03900"
     CDS             1216..1842
                     /gene="rplC"
                     /locus_tag="VNL92_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWO93886.1"
                     /translation="MALPGLLGRKIGMTQVFRPDGTVEPVTVIQAGPNVVTQLRTVEK
                     DGYEAVQVGFGDKKRLNKPERGHLGNRGNLRHLREFRGPVEGVSVGDHIGPEILEPGM
                     LVDVTARSKGRGFAGVVKRHGFHGGPKTHGQSDRHRAPGSIGSGTTPGRVFKGLKMAG
                     HMGAENVTVRALEVLEVDPARGLLLVKGSIPGARNGLVRVRRSTKQRR"
     gene            1849..2517
                     /gene="rplD"
                     /locus_tag="VNL92_03905"
     CDS             1849..2517
                     /gene="rplD"
                     /locus_tag="VNL92_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWO93887.1"
                     /translation="MELPVVDRTGTSVGTIEVADEVFGLAPNRSVLHQTFVAQMANRR
                     QGTVETKTRGQVRGSTVKIRRQKGLGAARQGSIRAPHRTGGGIVFGPHKRDFSKSLPK
                     RMRRLAIRSALSSKARDGELVIVDELTMATPKTKDIVALLEALDVRGSTIIATGQPDE
                     NVKRSAANLERVRVMPAAYLNVADMMNHRHLILTKDAVRAAESLWGGERANLRRAPLA
                     GGTR"
     gene            2527..2823
                     /gene="rplW"
                     /locus_tag="VNL92_03910"
     CDS             2527..2823
                     /gene="rplW"
                     /locus_tag="VNL92_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWO93888.1"
                     /translation="MPKAIHPYTVLLRPIITEKSTVLAEQGKYVFAVAVGANKLQIKD
                     AVELAFDVTVEAVNVMNVRGKTHRFGRRTSKSPDWRKAVVTLAPGDRIELFEGV"
     gene            2826..3659
                     /gene="rplB"
                     /locus_tag="VNL92_03915"
     CDS             2826..3659
                     /gene="rplB"
                     /locus_tag="VNL92_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWO93889.1"
                     /translation="MPIRPYKPTSPGRRGASVNTFDGLAKKRPEKTLVAGPNKRTSGR
                     NNQGRITTRHRGGGPKRLLRSIDFRREKHGVPGRVASIEYDPNRSARIALIVYRDGDK
                     RYILAPDGLKVNDIVTSGPEAEFRVGNSLPLRNIPTGTMVHNIELVAGKGGQMVRSAG
                     ASAQLMAKEGDYCLLRLPSGEMRRVRSECLATIGQVGNVEHSNVKLGKAGRTRHRRRR
                     PQVRGSAMNPPDHPHGGGEGKAPIGLPGPKSPWGKPTLGHRTRKNKSTDKFIVRSRHA
                     R"
     gene            3664..3951
                     /gene="rpsS"
                     /locus_tag="VNL92_03920"
     CDS             3664..3951
                     /gene="rpsS"
                     /locus_tag="VNL92_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWO93890.1"
                     /translation="MSRSTKKGPYVHEKLARKVEMVARSGQKQVIRTWARASTITPQM
                     VGMTIAVHDGRRHVPVYITENMVGHKLGEFAPTRTFRGHVSKSERTAKVRR"
     gene            3965..4303
                     /gene="rplV"
                     /locus_tag="VNL92_03925"
     CDS             3965..4303
                     /gene="rplV"
                     /locus_tag="VNL92_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWO93891.1"
                     /translation="MQVRAVAKNVHVSPRKARLVLRELPGKSVGEAMTMLRFTPVPIA
                     RDIAKVVQSAAANAENNYGLDTEDLRIVRATVDDAMRLRRFKAKARGRAAPRIRRFSH
                     ITVVVEDTEA"
     gene            4303..5022
                     /gene="rpsC"
                     /locus_tag="VNL92_03930"
     CDS             4303..5022
                     /gene="rpsC"
                     /locus_tag="VNL92_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWO93892.1"
                     /translation="MGQKVNPTGFRIGVIYDWQSKWYADKAYTDLLHEDLAIRNQVAK
                     QLADASVSKVEIDRSAHQATVTIHTAKPGIVIGRGGQRVDELRNRLEALTGHRVRVNI
                     HEINQPETDAYLVARSVAEQLQRRVAFRRAIKQAAQRTMQRGALGVKIRVAGRLGGSE
                     MSRAETERQGRVPLHTLRADIDYGFTEAHTTFGRIGVKVWIYKGDILPERRKEPEIAE
                     PAAAPVEGEEAAAAPASAVEG"
     gene            5044..5466
                     /gene="rplP"
                     /locus_tag="VNL92_03935"
     CDS             5044..5466
                     /gene="rplP"
                     /locus_tag="VNL92_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWO93893.1"
                     /translation="MLQPRRVRYRRSHRGRRAGPANSGNSVTFGEFGLQALENAWIDA
                     RQIEAARRSITHHMKRGGKVWIRIFPDKPVSKKPAETRMGSGKGPPDHWVAVVKRGRM
                     MFELAGVREDVAREAMRRAGQKLPIATRFVVREQEPVA"
     gene            5463..5672
                     /gene="rpmC"
                     /locus_tag="VNL92_03940"
     CDS             5463..5672
                     /gene="rpmC"
                     /locus_tag="VNL92_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWO93894.1"
                     /translation="MTRKADELRSLSDEDLAKRLEESYRQLFTLRFQVATRQLGNHRA
                     LRDTRRRIARIKTLQRERELAAQEG"
     gene            5674..5916
                     /gene="rpsQ"
                     /locus_tag="VNL92_03945"
                     /pseudo
     CDS             5674..5916
                     /gene="rpsQ"
                     /locus_tag="VNL92_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018347772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            6129..6497
                     /gene="rplN"
                     /locus_tag="VNL92_03950"
     CDS             6129..6497
                     /gene="rplN"
                     /locus_tag="VNL92_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWO93895.1"
                     /translation="MIQQETRLKVADNSGAREILCIRVLGGTRRRYARVGDIIVASVK
                     TAQPNSSVKKGDVVRAVIVRTKKPYGRSDGSHIRFDENAAVILSDKLNPRGTRIFGPV
                     GRELRERNFMKIVSLAPEVV"
     gene            6499..6813
                     /gene="rplX"
                     /locus_tag="VNL92_03955"
     CDS             6499..6813
                     /gene="rplX"
                     /locus_tag="VNL92_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWO93896.1"
                     /translation="MRRIKRNDTVRVMKGRDRGRQGVVRQVVTDEDRVVVTGINMVKR
                     HARARGLNQPAGIIELEAPLHVSNIRLVCPQCGKPTRVGFRLQANGAKVRYCKQCDAD
                     VD"
     gene            6825..7376
                     /gene="rplE"
                     /locus_tag="VNL92_03960"
     CDS             6825..7376
                     /gene="rplE"
                     /locus_tag="VNL92_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWO93897.1"
                     /translation="MAELPELKRRYREEIVPAMQREFDYRNVMQVPRLTKIVVNIGLG
                     EALQNARALDAASTDVATITGQKPVITRAKTSISNFKVREGNPIGIMTTLRGDRMYEF
                     FHRLVSAALPRIRDFGGVSPDAFDGRGNYSLGVREQLIFPEIEYDQVDRIRGLQITIV
                     TSAETDEEARRLLQLFGMPFSRS"
     gene            7386..7571
                     /locus_tag="VNL92_03965"
     CDS             7386..7571
                     /locus_tag="VNL92_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWO93898.1"
                     /translation="MAKVSQIVKAQRTPKFSSRRKHRCRLCGRPRAYMRQFALCRICF
                     RELAHRGQLPGVQKSSW"
     gene            7586..7984
                     /gene="rpsH"
                     /locus_tag="VNL92_03970"
     CDS             7586..7984
                     /gene="rpsH"
                     /locus_tag="VNL92_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWO93899.1"
                     /translation="MAINDPIADMLTRIRNAVAAKHELVQMPSSKLKVAIANVLKEEG
                     FIRDYEVQEQDSKRMLRIKLAYTGKKDSVLSGLKRVSRPGLRVYVQKGEIPRVYGGLG
                     VAILSTPQGVMTGQQAWARRIGGEVICYIW"
     gene            8000..8551
                     /gene="rplF"
                     /locus_tag="VNL92_03975"
     CDS             8000..8551
                     /gene="rplF"
                     /locus_tag="VNL92_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWO93900.1"
                     /translation="MSRIGKLPITVPSGVDVELGDGSHVRVRGPKGTLERDLPRDMTL
                     TIDDGTVSVARPSDAPQHRALHGLTRTLVYNMVAGVSEGFTKTLEVQGVGYRAQMQGK
                     VLALQLGYSHPITVEPPEGITLEVEGNRVRVSGIDKELVGQVAANIRALRPPQVYTGK
                     GVRYEGERVRRKAGKAGKGAGRR"
     gene            8573..8938
                     /gene="rplR"
                     /locus_tag="VNL92_03980"
     CDS             8573..8938
                     /gene="rplR"
                     /locus_tag="VNL92_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWO93901.1"
                     /translation="MQKTKTRRAARIRRHVRIRRRLEGTPERPRLAVFRSQTHIYAQI
                     IDDAARRTIVAASDVEPELRKNGAEGKTKRAAIVGQRLAERAKAAGITEVVFDRGGFK
                     FHGRVKALAEAAREGGLKF"
     gene            8952..9554
                     /gene="rpsE"
                     /locus_tag="VNL92_03985"
     CDS             8952..9554
                     /gene="rpsE"
                     /locus_tag="VNL92_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWO93902.1"
                     /translation="MVMAATRGTREGRRGDRGEQRDGDLQEKVVYINRVAKVVKGGRR
                     FNFSALVVVGDGHGSVGAGLGKANEVPEAIRKASTIAKRNLIQVPMRETTLPHSALVN
                     YGAAKVLLKPASRGTGVIAGGGVRAVLEMAGIRDVLAKSLGSNNPVNVVRATMKGLAS
                     MRDPERERLRRHALREEQESPRAMRPAKAEQQPEEADDGE"
     gene            9544..9744
                     /gene="rpmD"
                     /locus_tag="VNL92_03990"
     CDS             9544..9744
                     /gene="rpmD"
                     /locus_tag="VNL92_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWO93903.1"
                     /translation="MASKLRVTWKRSGIGQRTDQRRTVRALGLHRLGQSVEHADNPTI
                     RGMVHKVRHLVVVEELDDVERT"
     gene            9763..10233
                     /gene="rplO"
                     /locus_tag="VNL92_03995"
     CDS             9763..10233
                     /gene="rplO"
                     /locus_tag="VNL92_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWO93904.1"
                     /translation="MQAHQLRPDRGARHARKRIGRGNASGQGTYSGKGIKGQKARSGN
                     DLRVGFEGGQLPLVQRLGRKRGFTNIFRVEYQPVNVGRLAAYAAKTDATEVTPEALRS
                     AGIVKHLRQPVKVLGAGDIDRALTVRAHAVSAEARRKIEAAGGSVEEVAYASGR"
     gene            10217..11554
                     /gene="secY"
                     /locus_tag="VNL92_04000"
     CDS             10217..11554
                     /gene="secY"
                     /locus_tag="VNL92_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWO93905.1"
                     /translation="MQAGARPQARQGSPRLLQAVVDAFQQADVRAKLLFTLAMLVIFR
                     FVAHVPVPNVDSDVLDQVFDDNAALGFLDIFSGGALRNLSIAALGVYPYISASIILQL
                     LTPVVPRLQALSQEGEQGRNRIQLYTYWLTVPLAMVQGYAQLIILERNGAIANIGFTE
                     GDALPTLATILAMTGGTMFLVWLGELITERGIGNGLSIIIFGGIVAGFPGLIGNVLDS
                     NVSFGGVAILAVLAVVMIAAIVYVQEAQRRIPVQYARSMFRSGRMYRQSGQSHIPLRV
                     NSAGMIPLIFAYSIMIFPTTAAQFFEASDTGWVRDTADFIGNVFDPTNPVYWTLVFLM
                     VVAFTFFYTMVVFQQQNLAENLQKQSGFIPGIRPGRPTQEYITRVLTRITWGGALFLG
                     LMAIIPYAATRFMDVNALTISSTGLLIVVGVVLDTMRQLEAQLLMRNYEGFIR"
     gene            11590..12219
                     /locus_tag="VNL92_04005"
     CDS             11590..12219
                     /locus_tag="VNL92_04005"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWO93906.1"
                     /translation="MLLGPPGAGKGTQAERIKNEFAVGHLSTGDMFREEVAAGTELGQ
                     RAKAYMDRGELVPDELTIEMLLQRLFQPEYASGVLFDGFPRTLEQAKALDRALGERGR
                     HIDAVLYLSIPDEEALTRLSGRWICRQCGAVYHERNNPPRHPGRCDQCGGELYQREDD
                     TRETAMRRLERQKPPQDLLAYYREQGKLVEVDGMRSVEEVGGDLVAALR"
     gene            12277..13044
                     /gene="map"
                     /locus_tag="VNL92_04010"
     CDS             12277..13044
                     /gene="map"
                     /locus_tag="VNL92_04010"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWO93907.1"
                     /translation="MAVELKSPQEIEVMREAGRIVAEVLIILKEAVRPGVAERELDEI
                     VRREFKRRGAIPTFLNYSPRRGLTPYCATVCVSVNDEIVHGIPGDYAFREGDVVSIDL
                     GATYKGFVGDSAITIGVGNVSEEAERLIRATEESLHRGIEAARVGARLGDVSAAIQEV
                     GERYGYGIVREYGGHGVGREMHEDPFVPNYGVPDRGMELREGMVLALEPMLNAGDWRT
                     KVDPDQWTVRTADGSLSAHFEHTVAVTRDGPVVLTSP"
     gene            13068..13334
                     /gene="infA"
                     /locus_tag="VNL92_04015"
     CDS             13068..13334
                     /gene="infA"
                     /locus_tag="VNL92_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWO93908.1"
                     /translation="MARKGRQTTAEARGPRVAKKDVIEVEGIVKEPLPNAMFTVELAN
                     GHEVLAHISGKIRMHFIKILPGDRVLVELSPYDLTRGRITYRFK"
     gene            13351..13464
                     /gene="rpmJ"
                     /locus_tag="VNL92_04020"
     CDS             13351..13464
                     /gene="rpmJ"
                     /locus_tag="VNL92_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWO93909.1"
                     /translation="MKVQASVRPRCEKCKVIRRHGRVMVICEIPKHKQRQG"
     gene            13480..13860
                     /gene="rpsM"
                     /locus_tag="VNL92_04025"
     CDS             13480..13860
                     /gene="rpsM"
                     /locus_tag="VNL92_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWO93910.1"
                     /translation="MARIAGVDIPRDKRVEVALTYIYGIGPRSSKEILTTTQVSPDAR
                     VRDLTDEEVARIREAIERNYVVEGDLRREVRQNVQRLIEIGCYRGIRHRRGLPVRGQR
                     TRTNARQKRGTRRTVAGKRKAVKK"
     gene            13891..14280
                     /gene="rpsK"
                     /locus_tag="VNL92_04030"
     CDS             13891..14280
                     /gene="rpsK"
                     /locus_tag="VNL92_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWO93911.1"
                     /translation="MADRTTRGRRRERKSVPRGKAFIRSTFNNTIITLTDPNGNVVAW
                     GSSGSAGFKGSRKGTPYAAQLAGENAAKKAMEHGMREVEVLVRGPGSGREAAIRSLQA
                     AGLNVVRIRDITPIPHNGCRPPKRRRV"
     gene            14307..14930
                     /gene="rpsD"
                     /locus_tag="VNL92_04035"
     CDS             14307..14930
                     /gene="rpsD"
                     /locus_tag="VNL92_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWO93912.1"
                     /translation="MARYTGPVCRLCRREGQKLFLKGEKCYTKCTIERRPSPPGQRPA
                     RRRKVSDRALQLREKQRAREVYGVLERQFRRYHEQAVHQKGVTGDNLVRLLEGRLDNV
                     VYRLGWADSRNQARQIVRHGHITLNGRKTDVPSAQVRPGDTIGWSPRGQRSFFFDAAK
                     QNAPGKSVVPWLSADAAAMTGRVLAEPDPAEAGLLFDPSIIVEYYSR"
BASE COUNT         2906 a         4490 c         5294 g         2350 t
ORIGIN      
        1 tggtggcggc gccggagggg ccgatgccgc agacgcggga gcacattctg ttggcgcggc
       61 aggtggacgt tccgaacatc gtggtgtttc tgaacaaggt cgacatgatg gacgacccgg
      121 agttgctgga ccttgtggag ctggagttgc gggagctcct gacgaactac gagttccctg
      181 gggacgaggt gccgatcatc cgcgggtcgg cgttgcaggc gttggagagt acgagcacgg
      241 acccaggggc gccggagtac gcgcccatct gggagctgat gaaggcggtg gacgagtaca
      301 tcccgacgcc ggagcggccg cgggaccagc cgttcctgat gccgatcgag gacgtgttcg
      361 gcatcaaggg acgagggacg gtcgtgacgg ggcggatcga gcgcggcgtc gtgaaggttg
      421 gcgaggaggt ggagatcgtc ggcctgaggg agacgcgcaa gacgacggtg acgggtgtgg
      481 agatgttcaa gaagctcctg gacacgggcg aggcggggga caacgtgggg tgccttctgc
      541 ggggcatcga gcgggacgac gtcgagcgtg gccaggtgct ggcgaagccg gggacgatca
      601 cgccgcacac gaagttcgag ggggaggtct acgtcctgtc gaaggaagag ggagggcggc
      661 acacgccatt cttcacgggg taccggcccc agttctacat tcggacgacg gacgtgacgg
      721 ggtcgattgc gttgccggag ggcgtggaga tggtgatgcc cggggacaac gtgaagatga
      781 cgatcgagct gatccagccg atagcgatcg aggacggctt gcggttcgcc attcgcgagg
      841 gcggccggac cgtgggcgcc ggcgtggtaa cgaaggttct gcagtaacca tggcacgagg
      901 cgacacggca acacggcgca agatccgcat ccgccttaag gcgtacgatc accgtatcct
      961 cgaccagtcg gcggtacaga tcgtcgaagc cgcggagcgc acgggtgcgg ccgtcgccgg
     1021 gccggtgccg ctaccgacgc acatcaagaa gttcacggtc aaccgctctc cgttcatcga
     1081 caaggattcg cgcgagcagt tcgagatacg gacgcacaag cgactcatcg acatcctcga
     1141 tccaacctcg cgcacggtcg acacgctgat gcggttgcag ttgccttctg gcgtagatat
     1201 cgagatcaag ctctgatggc gcttccagga ttgctaggac gcaagatcgg catgacgcag
     1261 gtgttccggc cggacggtac ggtggaaccc gtcaccgtca tccaggctgg ccccaacgtc
     1321 gtcacccagc tccgcaccgt cgagaaggac gggtatgagg ccgtgcaagt cggattcgga
     1381 gacaagaagc gcctgaacaa accggaacgg ggccacctcg ggaaccgggg aaacctgcgc
     1441 cacctccggg agttccgcgg tcccgtggag ggcgtgagcg tgggtgacca catcggtccg
     1501 gagatcctgg aacccggcat gctggtcgat gtgacggcgc gttccaaggg ccgaggcttc
     1561 gccggcgtgg tcaagcgaca tgggtttcat ggaggaccga agacacacgg ccagtccgac
     1621 cggcatcgcg ctcccggttc catcggttcg ggcacgacgc cgggccgtgt gttcaaggga
     1681 ctcaagatgg cgggccacat gggtgccgag aacgtgaccg tacgggcact cgaggtgctc
     1741 gaggtcgacc cggcgcgagg cctgctcctg gtcaagggca gcatcccggg cgcccggaac
     1801 ggcttggtgc gcgtgcgacg ctcgacgaag cagcgaaggt aggcagaggt ggagctcccc
     1861 gtcgtcgatc gcactggaac gtccgtcggc accatcgagg tggccgacga ggtgtttggc
     1921 ttggcgccga accgctccgt gctgcaccaa acgttcgtcg cgcagatggc gaaccgccgc
     1981 cagggaaccg tcgaaacgaa gacgcgcggt caggtgcgcg gctcgaccgt caagatcagg
     2041 cggcagaagg ggctcggggc cgcgcgtcaa ggctccatcc gggcgccaca tcgaacgggg
     2101 ggtggcattg tcttcggccc gcataagcga gacttctcga agagcttgcc gaagcgcatg
     2161 cgccgcttgg ccatccgctc ggcgctttcc tcgaaggcac gcgatggtga gctcgtcatc
     2221 gtcgacgagc tcacgatggc gacgcccaag acgaaggaca tcgtcgcact cctcgaggcg
     2281 ctcgacgttc gcggatcgac gatcatcgcc accgggcagc cggacgagaa cgtgaagcgc
     2341 agcgccgcca acctggagcg ggtgcgcgtg atgccggccg cgtacttgaa cgtcgcggac
     2401 atgatgaacc atcgtcacct catcctgaca aaggatgcgg tgcgggcagc ggagtcactt
     2461 tggggcggcg aacgcgcgaa cctgcgtcgc gcgccgctcg ccggtggaac gcggtaggga
     2521 agcgagatgc cgaaagcgat tcatccgtac acggtcctcc tccgccccat cattacggag
     2581 aagagcacgg tgcttgccga acaggggaag tacgtgttcg ccgtcgctgt cggcgccaac
     2641 aagctccaga tcaaggatgc cgtcgagttg gcgttcgatg tgacggtcga ggccgtcaac
     2701 gtgatgaacg ttcgcggcaa gacgcaccgg ttcgggcgcc gcacgagcaa gagccccgat
     2761 tggcggaagg ccgtcgtgac gctcgcgccc ggcgatcgca tcgagctgtt cgagggagtg
     2821 taggcatgcc gatccgcccc tataagccga cctcgccggg ccgccgcgga gcatcggtca
     2881 atacattcga cgggcttgcc aagaagcgtc ccgaaaagac gctcgtcgcg ggccccaaca
     2941 agcgcacatc cgggcgcaac aaccagggac gcatcaccac gcggcaccgt gggggcggtc
     3001 cgaagcgatt gttgcgaagc atcgactttc gccgggagaa gcacggcgtt ccgggcaggg
     3061 tggcgtcgat cgagtacgac ccgaatcgct ccgcccgcat cgctctcatc gtgtaccgcg
     3121 atggggacaa gcgctacatc ctggcgccgg acggcctgaa ggtgaacgac atcgttacct
     3181 ccggccccga ggccgagttc cgcgtgggga acagcctgcc cctgcgcaac ataccgaccg
     3241 gcaccatggt gcacaacatc gagttggtgg cgggcaaggg tggtcagatg gtgcggagcg
     3301 ccggtgcctc ggcacagctg atggcgaagg agggcgacta ctgtcttcta cgattgccct
     3361 ccggcgagat gcggcgtgtc cgcagcgagt gcctcgcgac catcggccag gtcggcaacg
     3421 tggagcatag caacgtgaag cttggcaagg ccgggcgcac gcggcatcgc cggaggcgtc
     3481 cgcaagtccg cggttcggcg atgaatccgc cggaccaccc ccacgggggc ggcgagggga
     3541 aggcgcccat cgggctgccg gggccgaagt cgccgtgggg caagccgacg ttggggcatc
     3601 gcacgaggaa gaacaagtcc acggacaagt tcatcgtgcg cagccggcac gcgaggtaac
     3661 cgaatgtcgc gttcgacaaa gaagggaccg tacgttcacg agaagctcgc ccgcaaggtg
     3721 gagatggtgg cgcggtccgg gcagaagcag gtgattcgga cctgggcgcg ggcgtccacc
     3781 attacgcccc agatggtggg catgacgatc gcggtgcacg atggacgccg ccatgtcccc
     3841 gtatacataa cggagaatat ggtgggccac aagctcggag agtttgcgcc gacccgcacg
     3901 ttccgggggc acgtgagcaa atcggagcgc acggcgaaag tacgccgtta ggagctgagg
     3961 acgagtgcag gttcgagctg ttgcgaagaa cgttcacgta tcgccgcgga aggcgcgcct
     4021 cgtgttgcgc gaactcccgg gcaagagcgt tggcgaggcg atgacgatgc tgcgattcac
     4081 gccggtgccg atcgctcgcg acatcgcgaa ggtggtccaa tcggcggcgg cgaacgccga
     4141 gaacaactac ggcctcgaca cggaagacct tcgcatcgtt cgggcaacgg tcgatgatgc
     4201 gatgcgcttg cggcgcttca aggcgaaggc gcgcggacgc gccgcccctc gcatcaggcg
     4261 cttcagccac atcaccgttg tggtcgagga cacggaggcg taatgggaca gaaggtcaac
     4321 ccaaccgggt tccgcatcgg cgtcatctac gactggcaga gcaagtggta cgcggacaag
     4381 gcgtatacgg accttctgca cgaggatctc gccatccgga accaggtggc gaaacagctc
     4441 gccgatgcta gtgtctccaa ggtcgagatc gaccgcagcg cgcaccaggc gacggtcacg
     4501 atccacacag cgaagcccgg catcgttatc ggccggggcg gacagagggt cgatgagttg
     4561 cgtaaccggc tcgaggcgct cacggggcac cgcgtgcgcg tcaacattca cgagatcaac
     4621 cagccggaaa ccgacgcgta cctcgttgcc cggagcgtcg cggagcagct gcaacggcgg
     4681 gtcgcctttc gccgggcgat caagcaggca gctcagcgga ctatgcaacg gggcgcgctc
     4741 ggcgtgaaga tccgcgtcgc gggccggctc ggaggctcgg agatgtcccg ggccgagacg
     4801 gagcgccagg gacgcgtgcc cctgcacacc ctccgcgccg acatcgacta cggcttcacg
     4861 gaagcccaca cgaccttcgg tcgcatcggg gtcaaggtct ggatctacaa gggcgatatt
     4921 ctaccggagc gccgtaagga acccgagatc gccgaacctg ctgcggcccc ggtggaaggc
     4981 gaagaggcgg cggccgcgcc ggcgagcgcg gtcgagggat agggaaggaa gggacgcttt
     5041 cccatgctgc aaccacgacg tgtacggtac cggaggtcgc atcgcggacg ccgtgctggc
     5101 cccgcgaact cgggcaactc cgtgacgttc ggcgagttcg gcctccaggc gctcgagaat
     5161 gcgtggatcg atgcacggca aatcgaggca gcgcgtcgct ccatcacgca ccacatgaag
     5221 cgcggcggca aggtgtggat tcgcatcttt ccggacaagc cggtctccaa gaagccggcc
     5281 gagacacgca tggggagcgg caagggaccg cccgatcatt gggtggccgt tgtgaagcgg
     5341 gggcgcatga tgttcgagct ggccggtgtg cgtgaagacg tcgcgcggga ggccatgcgc
     5401 cgcgcgggcc agaagctgcc catcgcaacg cgcttcgtcg tgcgcgagca ggagcccgtg
     5461 gcgtgacgag aaaggcggac gagttgcggt ccctctcgga cgaggacctc gcgaagcggc
     5521 tcgaggagtc gtatcggcag ctgttcacgc tgcgcttcca ggtggcgacg cggcagctcg
     5581 ggaatcatcg cgctctccga gacacgcgga ggcgcattgc gcgaatcaag acactgcaac
     5641 gcgagcgcga gcttgccgcg caagaaggtt gagatggaag cgaaccggaa gacccggcag
     5701 ggcgtggtag tgagcgacaa aaacgacaag accgtcgtcg tcgcggtcca gacgatcaaa
     5761 tcgcacaggc tgtacggcaa gcgcatgcgt cgtacgcagc ggctgcacgc gcacgacgag
     5821 agcaatgtgt gccgtctcgg cgacctggtt ctgctgcggg agacgcggcc actctcgaag
     5881 ctgaagcgct ggaccgtcgt cgagatcttg cgaaagggcg atgtcgcgga ggtgcagccc
     5941 caacagatcg gtcgagagtt ggaggtgcct gcgaggaccg aggaggagga gcgggcggaa
     6001 gaggtcgtgg ccggtggaac ggcgccccaa acggaagccc cggaggctga gctgccggcg
     6061 gcgctcgacg aagcgctcgg cgctgaggaa ggcgtggaag ctgagccgag cggagacgag
     6121 gcgacggaat gatccagcag gagacccggc tcaaggttgc cgacaactcg ggcgcccggg
     6181 agatactctg catccgcgtg ctcggcggca cgaggcgccg ctatgcgcgc gtgggcgaca
     6241 tcatcgtggc ctccgtaaag acggcccagc cgaactcctc ggtcaagaag ggcgatgtgg
     6301 tgcgtgccgt catcgtgcgc accaagaagc cgtacggccg gtccgacggc agccacatcc
     6361 ggttcgacga gaacgccgcc gtcattcttt cggacaagct caatccgcgg ggtacgcgca
     6421 tcttcgggcc ggttgggcgc gagttgcgcg aacgcaactt catgaagatc gtttccctcg
     6481 ctccggaggt cgtctagcgt gcgcaggatc aagcgaaacg acaccgtgcg cgtgatgaag
     6541 ggtcgcgacc gtggcaggca gggcgtcgta cgccaagtcg tcaccgatga agaccgcgtc
     6601 gtcgtgaccg gcatcaatat ggtcaagcgt catgcacggg ctcgcgggct caaccagccc
     6661 gcggggatca tcgagctcga ggcgcccctg catgtaagca acatcaggct cgtttgtcct
     6721 caatgtggaa agccgacccg cgtcggcttc cgcctacagg ccaacggcgc gaaggtgcgc
     6781 tactgcaagc agtgcgatgc cgatgtcgac taggacggat tcgaatggct gagctacccg
     6841 agctgaagcg acgctaccgg gaggagatcg tcccggcgat gcagcgcgag ttcgactacc
     6901 gcaacgtgat gcaggtgccg aggctcacga agatcgtcgt gaacatcggc ctgggcgaag
     6961 cgctgcagaa cgcgcgggca ttggatgccg cctcgacgga cgtggcgacg atcacgggac
     7021 agaagccggt gattacgcgc gcaaagacgt cgatatcgaa cttcaaggtg cgcgagggga
     7081 accccatcgg gatcatgacg acgttgcgcg gcgatcggat gtacgaattc ttccaccgac
     7141 tcgtctccgc cgcgttgccc cgcattcgcg acttcggcgg cgtatccccg gatgcgttcg
     7201 acgggcgagg gaactatagc ctcggtgtcc gggagcagct catcttcccg gagatcgagt
     7261 acgaccaggt agaccgcatc cgcgggcttc agataactat cgtgacaagc gctgagacgg
     7321 acgaggaggc gcggcgtttg ctgcagctct tcggaatgcc attcagccga agctaggagg
     7381 ccaacgtggc caaagtgtcg cagatcgtaa aagcgcaacg aacgccgaag ttctcgagcc
     7441 ggcgcaagca ccgctgccgg ctgtgcggcc ggccgcgcgc atacatgcga cagttcgcgc
     7501 tctgccgcat ctgctttcgg gagctcgctc accgcggaca gcttcccggt gtgcagaagt
     7561 cgagctggta ggagggaggg cacgcatggc aatcaacgac ccgatcgccg atatgctcac
     7621 ccgcattcgt aacgccgttg cggccaagca cgagctcgtg cagatgcctt cttcgaagtt
     7681 gaaggtagcc attgccaacg tgcttaagga agaagggttc attcgcgact acgaggtaca
     7741 ggagcaggac tcgaagcgaa tgctacgcat caagctcgcg tacaccggga agaaagactc
     7801 ggtgctcagc ggcctgaagc gcgtctcgcg tccggggctc cgtgtctacg tccagaaggg
     7861 cgagattccg cgcgtttacg gagggctggg cgtcgccatc ctttccacgc cgcaaggcgt
     7921 gatgacaggg caacaggcct gggctcgccg aatcggaggc gaggtcatat gctacatctg
     7981 gtaggacagc gggggaacga tgtcgcgcat cggtaagctg cccatcaccg tgccttcggg
     8041 cgttgatgtc gagcttggcg acggcagcca cgttcgtgtc cgcggaccga agggcacact
     8101 cgagcgggac ttgcctcggg acatgacgct gacgatcgac gacggcacag tgtcggtcgc
     8161 ccggccgtcc gatgctcctc agcaccgtgc gctgcacggt cttacgcgaa cgctcgtcta
     8221 caacatggta gcgggcgtct ccgagggctt cacgaaaacg cttgaggtgc agggcgtcgg
     8281 ttatcgagct caaatgcagg gaaaggtctt ggcgctccag ctcggctatt cgcatcccat
     8341 taccgtggag ccgcccgagg gcatcaccct cgaggtcgag ggcaaccgcg tgcgggtgag
     8401 cggcatcgac aaggagctcg tgggtcaggt ggcggccaac attcgcgcct tgaggccgcc
     8461 tcaggtgtac acgggcaagg gcgtccgtta cgagggcgag cgcgttcggc gcaaggcagg
     8521 caaggccgga aagggcgccg gacggcggta gcgccaatcg gggcgaaggt tcatgcagaa
     8581 gacgaagaca cgcagggcag cacgcatccg gagacacgtc cgcatccggc gccggttgga
     8641 aggcacgccg gagcggccga ggctcgccgt gtttcgcagc cagacgcaca tctatgcgca
     8701 gatcatcgac gatgccgcga ggcgcacgat cgttgcggct tccgatgtgg agccggagct
     8761 gcgcaagaac ggcgccgagg ggaagacgaa gcgggcagcc atcgtaggac agcgcctggc
     8821 ggagcgcgcg aaggcggcag ggatcaccga agtcgtcttc gatcgcggcg gattcaagtt
     8881 ccacggacga gtgaaggcgc tggcagaagc cgcgcgcgaa ggcggactga agttctagag
     8941 gattcgaacg tatggtaatg gcagctacgc gaggaacacg cgaaggccgg aggggcgacc
     9001 gcggcgaaca acgcgatggc gaccttcaag agaaggtcgt ctacatcaac cgtgtcgcca
     9061 aagtcgtgaa gggcggtcgg cggttcaact tcagtgccct cgtcgtcgtg ggcgatggcc
     9121 atggttcggt cggcgccggc ttggggaagg cgaacgaggt tcccgaggcc atccggaagg
     9181 cctcgaccat cgcgaagcgg aacctgattc aggtgcccat gagggagaca acgctgccac
     9241 actcggcgct cgtcaactac ggcgccgcga aggttctgct gaagccggca tcgcgcggga
     9301 cgggcgttat cgcgggcgga ggcgtccggg ccgtgctcga gatggccggc atccgcgacg
     9361 tcctcgctaa gtcgctcggc agcaacaatc ccgtgaacgt cgttcgcgcg acaatgaagg
     9421 ggctcgccag catgcgagac cccgagcgcg aacgcctgcg acggcacgcc ctgcgcgaag
     9481 agcaggaatc gccacgcgcc atgcggccgg cgaaggcgga gcagcagcct gaagaggcgg
     9541 acgatggcga gtaagctccg tgtgacctgg aagcgcagcg gcattgggca gcgcacggat
     9601 cagcgccgca cagtccgcgc actcgggttg caccgtctcg gacagtccgt tgagcacgcg
     9661 gacaatccga ccattcgcgg catggtgcac aaggttcgcc atctcgtggt cgtcgaggag
     9721 ctcgacgacg tagagcggac gtaagggacg ggagggcggt tcatgcaggc gcatcagtta
     9781 cgaccggatc gcggagctcg gcacgcgcgg aaacgcatcg gccgaggaaa cgcgtccgga
     9841 cagggtacct actcgggcaa agggatcaag ggccagaagg cccgcagcgg caacgacctg
     9901 cgcgttggat tcgaaggcgg ccagttgccg ctggtgcagc gcctgggacg caagcgtggc
     9961 ttcactaaca tcttccgtgt cgagtaccag ccggtgaacg tgggcaggct cgctgcctat
    10021 gcggcgaaga cggacgccac ggaggtgacg ccggaggcgc tacgttcggc gggcatcgtg
    10081 aaacacctgc ggcagccggt gaaggtgctc ggcgccggtg acatcgatcg cgccctcacg
    10141 gtgcgggccc acgccgtctc cgccgaggcg aggcgcaaga tcgaagcggc tggaggctcc
    10201 gtcgaggaag tggcatatgc aagcgggcgc taggccgcag gcacggcaag gctcgccgcg
    10261 gcttcttcag gccgtcgtcg acgcgttcca gcaggccgac gtacgcgcga agctgctatt
    10321 tacgctcgcg atgctggtca tctttcgttt cgtggcgcac gttcccgtcc cgaacgtcga
    10381 ctccgacgtg ctcgaccagg tgttcgatga caatgcggca ctcggcttcc tcgatatctt
    10441 cagcggcggc gcactgcgta acctgagcat cgcagcgctg ggcgtgtacc cgtacatcag
    10501 cgcgtccatc attctgcagc tgctgacacc ggtggtaccg cgcctgcaag cgctttcgca
    10561 ggagggcgag caggggcgca atcgcatcca gctctacaca tactggctga cggtgccgct
    10621 cgccatggtc cagggatacg ctcaactcat catcctcgaa cgtaacggcg ccatcgcgaa
    10681 cattggcttt acggagggcg acgcgctgcc cacgctggcg acgatactcg cgatgacggg
    10741 cggaacgatg ttcctggtat ggctcggcga gctgatcacc gagaggggca ttggcaacgg
    10801 tctctccatc atcatcttcg gcggaatcgt ggccggcttc ccaggcctca tcggcaacgt
    10861 gctcgactcc aacgtgagct ttggcggcgt ggcgatcctc gccgtgctcg ctgtcgtgat
    10921 gatcgcggcc atcgtctacg ttcaggaggc gcagcggcgc attccggtgc agtacgcgag
    10981 aagcatgttc cgaagcgggc gcatgtaccg gcagtcaggg cagagccata tcccgctccg
    11041 cgtcaactcg gccggcatga ttccgctcat cttcgcctac tcgatcatga tcttcccgac
    11101 cacggcggcg cagttcttcg aggcgtcgga taccggttgg gtgcgtgaca cggcggactt
    11161 catcggcaac gtcttcgacc cgacgaatcc cgtctactgg accttggtat tcctgatggt
    11221 cgtcgcgttc acgttcttct acacgatggt cgtgttccag cagcagaact tggccgagaa
    11281 cctgcagaag cagagtgggt tcattcccgg catccggccc ggccgtccga cgcaggagta
    11341 catcacccgc gtgctcaccc gcattacgtg gggcggggcg ctgttccttg gcctgatggc
    11401 gatcatcccg tacgcggcca cgcggttcat ggacgtgaac gcactcacga taagcagcac
    11461 tggcctcctc atcgtcgttg gcgtggtgct cgataccatg cgccagctgg aggcgcagct
    11521 cctgatgcgg aactatgagg gcttcatccg ttagggcgga gctgcgacga gaggttggtc
    11581 ttggacatcg tgttgctggg cccgcccggc gcgggcaaag ggacccaggc ggagcgcatc
    11641 aagaatgaat tcgcggtcgg gcatctttcc acgggcgaca tgttccgcga ggaggtggcg
    11701 gccggcaccg agctcgggca gcgggccaag gcctacatgg accgcggcga gctggttccg
    11761 gacgagctta cgatcgagat gctgctccaa cgcttgtttc agcccgaata tgcctcgggc
    11821 gtcctgttcg atggattccc gcgaaccctc gagcaagcga aggccctgga ccgcgcactc
    11881 ggggagcgag gccggcatat cgatgcagtg ctctatctga gcatccccga tgaggaggcc
    11941 ctcacccgcc tttcgggccg ctggatctgc cgtcaatgcg gcgcggtgta ccacgaacgc
    12001 aacaatcccc cgcggcatcc tggacgctgc gaccagtgcg ggggcgagct gtatcagcgc
    12061 gaggacgaca cgcgagagac ggccatgcgc aggctggagc gtcagaagcc gccgcaggat
    12121 ttgctcgcct actaccgcga gcaaggcaag ctcgtcgaag tagacgggat gcgatcggtg
    12181 gaggaggtcg gcggcgatct ggtcgccgcg cttcgatagg gtgctgtacc atagagagga
    12241 atcatttata ctaagagatt gtcggccggg ggttggatgg cggtcgaatt gaagtcgccg
    12301 caggagatcg aggtgatgcg ggaggccggg cgtatcgtcg ccgaggtcct catcatcttg
    12361 aaggaggcgg tgaggcccgg ggtcgcagaa cgcgaactcg acgagatcgt ccggcgcgag
    12421 ttcaagaggc gaggcgcgat tccgacgttc ctgaactact ctccgcgccg gggtctcacg
    12481 ccctactgcg cgacggtctg cgtctctgtg aacgacgaga tcgtgcatgg cataccgggc
    12541 gactacgctt tccgcgaggg agacgtggtg agcatcgatc tcggggcgac gtacaagggg
    12601 ttcgtcggtg attcggcgat caccatcgga gttggcaacg tgagcgagga ggccgaacgc
    12661 ctcatccgtg cgacggagga aagcctgcac cgaggcatcg aggccgctcg cgttggcgcg
    12721 cggctcggag acgtctccgc agcgatccag gaggtcggcg aacggtacgg atacggcatt
    12781 gtgcgagagt acggcggcca cggggtcggt cgagaaatgc acgaggaccc attcgtgccg
    12841 aactatggag tgcccgatcg cggcatggag ctgcgcgagg gaatggtcct ggcgttggag
    12901 ccgatgctca acgcgggcga ttggcggacc aaggtggacc cggaccagtg gaccgtgcga
    12961 acggcggacg gcagcctttc cgctcacttc gagcatacgg tggccgttac gagggatggc
    13021 ccggtcgtgc tcacctcgcc ataggcggag cgtagtaagg gaggattatg gcacgcaagg
    13081 gacggcagac gacggcggag gcgcgtgggc ctcgtgttgc caagaaggac gtcatcgagg
    13141 tcgaagggat tgtcaaggaa cccctgccga acgcgatgtt cacggtcgag ttggcgaacg
    13201 gtcacgaggt gctggcgcac atttccggga agatccgcat gcactttatc aagatcctcc
    13261 cgggcgaccg cgtgcttgtc gagctttcgc cctacgatct cacgcgcggc cgcatcacct
    13321 atcggttcaa gtgacagcgg agagggcgac atgaaggttc aggcttcggt tcggccgcgc
    13381 tgcgagaagt gcaaggtgat tcgcaggcat ggccgcgtca tggttatctg cgaaatcccg
    13441 aagcacaagc agcggcaggg ctaggcacgg aggcaaggca tggcacgcat cgccggggtg
    13501 gatattccca gggacaagcg ggtcgaggtt gcgctgacct acatctacgg catcgggccg
    13561 aggtcgagca aggagatcct cacgactacg caggtgagtc ctgatgctcg cgtgcgcgat
    13621 ctcacggacg aagaagtggc ccgaatccgc gaggcgatcg agcgcaacta tgtggtggag
    13681 ggtgatctcc gccgcgaggt acgccagaac gtgcagcgac tgatcgagat cgggtgctat
    13741 cgcggcatcc gccaccggcg ggggctgccg gttcgcggtc agcgtacgcg cacgaacgcg
    13801 cgccaaaagc gcggcacgcg gcgcaccgta gccggcaagc ggaaggctgt gaagaagtag
    13861 gttcgggcgg aaaggcggag gcagcggagc atggcagatc gcacaacgcg ggggcgcaga
    13921 cgggagcgga aatcggtccc gcgtgggaag gcgttcatcc gctcgacgtt caacaacacg
    13981 atcatcacgc ttaccgaccc gaacgggaac gtcgtagcct ggggcagctc gggctcggcc
    14041 ggattcaagg gctctcgaaa gggaacgccg tacgctgcgc agctggccgg cgagaacgcg
    14101 gcgaagaagg cgatggaaca cggcatgcgc gaggtggaag ttctggtgcg cgggccggga
    14161 tcgggccgcg aagctgccat acgctcgctg caggccgctg ggctcaacgt ggtacggatc
    14221 cgtgatatca cgcccatccc gcacaatgga tgtcgtccgc caaagcggcg ccgggtctag
    14281 cggagcgtac gggaggaagg gatagcttgg ctcgatatac aggtccggtt tgccgcctat
    14341 gccggcgtga gggacagaag ctgttcctca agggcgagaa gtgctacacg aagtgcacca
    14401 tcgaacgccg cccttcgccg cctggtcagc gaccggcgcg gcgacggaag gtttcggacc
    14461 gcgccctgca gctgcgcgaa aagcaacgcg cccgcgaagt ctatggtgtg ctggaacggc
    14521 agttccggcg ctatcacgag caggcggtcc atcagaaggg tgtgaccggc gacaacctcg
    14581 ttcgcctgct ggaggggcgg ctcgataacg tggtctatag gctcggatgg gccgactcga
    14641 ggaaccaagc gcggcagatc gtccggcatg ggcacatcac gctcaacggc cggaaaacgg
    14701 atgtcccctc ggcgcaggtg cggccgggcg acacgattgg ttggagtcca aggggccagc
    14761 gatcgttctt cttcgatgcc gccaagcaga atgcgccggg caagtcggtc gtaccctggc
    14821 tcagcgccga tgctgctgcc atgacgggcc gcgtgttggc cgagccggac ccggccgagg
    14881 ccgggctcct gttcgacccg agcatcattg tggagtacta ctcgcgctag cccggccctg
    14941 ctgggcttcc gcttctgtgg gccgctcgcg ttctcggcgc ttgagcggcg tccgcacggc
    15001 gggcctctca cataaggaag gtggttccgt tgcaaccgca
//
LOCUS       DATMBB010000184         2300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_443352, whole genome shotgun sequence.
ACCESSION   DATMBB010000184 DATMBB010000000
VERSION     DATMBB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443352"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VNL92_04040"
     CDS             complement(<1..372)
                     /locus_tag="VNL92_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HWO93913.1"
                     /translation="MKRIFWGATITIGIMVAAWIAASVPSRAADQVDRVARGKYLVEG
                     IGCSDCHTPKKMGPQGMELDTTRLLAGHPEGTPLPEAPTGQSAWAIAATGDLTAWTGP
                     WGVSYAANLTPDQNTGLGIWTE"
     gene            complement(489..>2300)
                     /locus_tag="VNL92_04045"
     CDS             complement(489..>2300)
                     /locus_tag="VNL92_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWO93914.1"
                     /translation="ICQLLCRVAQARPGTSGQRTAVGSHRSRSASRASAPSTPCTEYA
                     EYTVAVMQHAGTSHPVERGWSCLKNREWPEARAAFQEAVDLDPSSGSAWEALGAAAMF
                     LDYGAGSRKAHERAYREYLERGDFRGAARVAIQLALYHDAYRGELAIASGWFERARSL
                     LATVPPSPEHAWLAFWKAHLDIHVHGEVARGEPSLEEALRLSEQTGPGTELELMMRGL
                     RGLMAISEGAIDEGLRRLDEATTAVTAEKLPTPQTIGWTYCYVLDACENVRDIDRASQ
                     WIDRAFEMERDLGIQHLMGACREHYIGILTWRGDYAGAERQIESMRREVEGIAPMFVA
                     RSDIRLGEIRRRQGRLEEAAALLEPQVAKPSAMLSLAWLALDRGSPRLAVDLIERYFR
                     RVSDGDRIRRLHGLDVLTRATIALGDTRAARAALDAHGEVVRRSATPMMQATACELQG
                     LVRVAEGDLDEGRKLLEDAVDGYSLTRAPYEGLVARLALGDVLKALGRVDSAEGVLQA
                     ALSGAREIGATRLAQRASDALAGERAERRPTALLTEREIEVLALVAQGISNQEIGERL
                     FISAYTVKRHIANILNKLDLPTRAAAAAYAIREGLAR"
BASE COUNT          325 a          828 c          768 g          379 t
ORIGIN      
        1 ctcggtccag atgccgaggc cggtgttctg gtcgggggtc aggtttgccg cgtagctcac
       61 gccccacggt cccgtccacg ccgtcaggtc ccccgtggcg gcaatcgccc acgccgattg
      121 accggtcggc gcctccggca gcggcgtgcc ctcaggatgg ccggccagca ggcgagtcgt
      181 gtcgagctcc atgccctgcg gccccatctt cttgggcgtg tgacagtcgc tgcatccgat
      241 cccctcgacc aggtacttcc cgcgcgcgac gcgatcgact tgatcggccg cccgactggg
      301 aacactcgct gcgatccacg ccgcaaccat gatcccgatg gtgatggtgg ctccccagaa
      361 gatgcgcttc attgtcgtcc tccaatcgtc ggttccggag gactgtagat gccgtcaggg
      421 gcgttgcgcg tcgccccgtc ggaccaattt gacagcggcc gtcgattggt ccgatcggac
      481 cacggcgatc agcgggcgag cccttcgcgg attgcgtacg ccgcggcggc ggcccgtgtc
      541 gggaggtcga gcttgttgag gatgttggcg atatggcgct tgacggtgta cgcgctgatg
      601 aacagccgct cgccgatctc ctggttgctg atgccctgcg cgacgagcgc gagcacctcg
      661 atttcgcgct cggtgagcag ggcagtcggc cggcgttccg cgcgctcccc ggccagcgcg
      721 tccgacgcgc gctgggcgag ccgggtcgcg ccaatctcgc gcgcgccgga gagagccgcc
      781 tggagcacac cctccgccga atcgacgcgg ccgagcgctt tcaggacatc tccgagcgcc
      841 agcctggcga cgagcccctc gtacggggcg cgcgtgaggc tgtagccgtc gacggcatcc
      901 tcgagcagct tccggccctc gtcgagatcc ccttcggcta ctctgacgag tccctggagc
      961 tcgcacgccg ttgcctgcat catcggcgtg gccgagcggc gcaccacctc gccgtgcgcg
     1021 tcgagcgcgg cccgcgcggc gcgcgtatct cccagcgcga tcgtcgcgcg cgtcagcaca
     1081 tcaagcccgt gcagccggcg gatccggtcg ccatccgaga cccggcggaa gtaccgttcg
     1141 atcagatcga cggcaagccg agggcttccc cggtcgagtg cgagccacgc gaggctgagc
     1201 atcgcggacg gcttggcgac ctgcggttcg agcagcgcgg cggcctcctc gagccgcccc
     1261 tgtcgcctgc ggatctcgcc cagacggatg tcgctgcgcg cgacgaacat cggcgcgatg
     1321 ccttcgacct cgcgacgcat gctttcgatc tggcgctcgg cgcccgcgta gtcgccgcgc
     1381 caggtcagga tgccgatgta atgctcgcgg cacgcgccca tgaggtgctg gattccgaga
     1441 tcgcgctcca tctcgaacgc gcggtcgatc cactgcgaag ctcgatcgat gtcgcgaacg
     1501 ttctcgcacg cgtcgagcac gtagcagtac gtccagccga tcgtctgcgg cgtcgggagc
     1561 ttctcggccg tcaccgcggt cgttgcctcg tccagccgcc ggagaccctc gtcaatcgct
     1621 ccttcgctga tcgccatcag gccgcgaagc ccgcgcatca tgagctccag ctcggtgccc
     1681 ggaccggtct gctcgctcag ccgaagcgct tcctcgaggc tgggctcgcc cctggcgacc
     1741 tctccatgca cgtggatgtc gagatgcgcc ttccagaacg cgagccacgc atgttcgggc
     1801 gagggtggga cggtagcgag caggctgcgc gcccgctcga accatccgct ggcgatcgcc
     1861 agctcgccgc gatacgcatc gtggtacagc gccagctgga tggcgacgcg cgcggcgccg
     1921 cggaaatccc cacgttccag atactcgcga tacgcccgct cgtgcgcctt gcggctccct
     1981 gccccgtagt ccaggaacat ggccgccgct ccgagcgcct cccaggcgga tcccgacgag
     2041 gggtcgagat cgaccgcctc ctggaacgcg gcgcgggcct cgggccactc gcgattcttc
     2101 agacacgacc agccgcgctc gaccgggtgg ctcgtaccgg cgtgctgcat gaccgccaca
     2161 gtgtactcgg cgtactcggt acacggcgta ctcggcgcag aggcgcggct cgcggagcgg
     2221 gagcggtgtg aacccacggc cgttcgctgc ccgctggttc caggtcgggc ctgggccacg
     2281 cgacagagca attgacatat
//
LOCUS       DATMBB010000185         5725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_1572, whole genome shotgun sequence.
ACCESSION   DATMBB010000185 DATMBB010000000
VERSION     DATMBB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VNL92_04050"
     CDS             <1..1111
                     /locus_tag="VNL92_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93915.1"
                     /translation="LVDGNHEYTVFNRAIGTGPFIMESYDTLGDGGRIKYVRNPDYFK
                     KGQPFFDAMELPIVTSSETIQAMFLQGDTDTSGTGLDATLTAALKEARPDMKTFEASG
                     FFLFKVYWDMANQDSPWARDVRMRQAMWYFMPYDALLNQLGVTCCRTGPMPPGQKPWA
                     LPNEDLPGSGLDLQSSIQEGLKLLDAAGYPAGTEIPLELSISNFYGGDLLGEVLTAIF
                     GGLKQASGGALNINVRLNVMELGSWLKDVYRGGGTFEATSHADAVFHDPDNGFYRYYH
                     STGVANNTHLNDPKVDDFLERQRREFDHEKRVEVVHEFQRYLLDISPTVYIVAPNSFS
                     GAQPWVENYKTHTIGYLETLRHWDELWFNETAPQR"
     gene            1159..2229
                     /locus_tag="VNL92_04055"
     CDS             1159..2229
                     /locus_tag="VNL92_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWO93916.1"
                     /translation="MKAAIVRQVGAKVEVVDVDVRDPGPGEVRLRIAATGVCHSDLSF
                     QNGTLPGMLPAILGHEGAGVVTHTGPGVTSVKPGDHVILNWVPSCGACYFCNRGQFQL
                     CGSPRGASEPKLFLDGVPIPGVAGMGTFAEEMVVSENAVVPIPDDVPMDVAALIGCGV
                     MTGAGAALNTAQVEPGSSVVVIGCGGVGINTIQGAKVCGAATILAVDRYENKREMAKR
                     FGATHAAAPEELPQAMQDLTAGRGFDYGFEVIGLAATIRQAWDATRRGGKTIVVGAGR
                     FDDMLQLSAFELFFMEKQLVGSVYGSANVRRDFPRLIALWRSGKLDLEGLVTRHVQLG
                     QVQEAFEAMERGEVIRQVVTFE"
     gene            complement(2216..3127)
                     /locus_tag="VNL92_04060"
     CDS             complement(2216..3127)
                     /locus_tag="VNL92_04060"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWO93917.1"
                     /translation="MTRVLVTGGAGFLGRHLVDALLARGDDVTILDNFRRSEPEGIPP
                     AVRLFEGDIRDPAAVQAAMEGAELVFHLAAQSNVMGSSADPDYAFATNVMGTYNVLRA
                     ARAAGVRRVVFSSSREAYGEPAALPVAEDTPLGAKNAYGASKIAGEAYCRVSEAEGGP
                     TCSILRFANVYGPGDRERVIPLWLDRAVRGEPLELYGGSQVLDFVWVGIAVECLLAAG
                     ERDLDGPVNVGSGTGTSLTELAERIRSLTGGRSELKVLPARPIEVTRFVADTTRMRRV
                     LGIQPPEDPLFALPSMLPLLRTTATRT"
     gene            complement(3124..4098)
                     /locus_tag="VNL92_04065"
     CDS             complement(3124..4098)
                     /locus_tag="VNL92_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWO93918.1"
                     /translation="MRIVIAGGAGFIGSNLTERLLAAGHSVICIDNFATGRPSNVEPF
                     LREAAFELIPHDIIEPLPPLPPVDRVYHLASPASPPGYVRLPIETMRANSEGTRHLLD
                     LSVRNGATFVYCSTSEVYGDSLEHPQTEAYRGNVSTTGPRSVYDESKRFGEALVAAYH
                     RRFDADVRIARIFNTYGPRSAPDDGRLVPNFVVAALRDKPITVYGDGTQTRSLCYVSD
                     LVEGLIAMAESDAAGGEVVNLGNPDEHTILEFAELVRELTGSRSEIVFRDYAVADDPR
                     RRQPDITKARRLLGWEPIVGLREGLTRTIDYFRAELGLAMSAEGRSRL"
     gene            4268..4344
                     /locus_tag="VNL92_04070"
     tRNA            4268..4344
                     /locus_tag="VNL92_04070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4302..4304,aa:Tyr,seq:gta)
     gene            4381..5556
                     /locus_tag="VNL92_04075"
     CDS             4381..5556
                     /locus_tag="VNL92_04075"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014127496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWO93919.1"
                     /translation="MTIGSQPSGLTADVDQRLALISRGAEEIMTRDELRKLLAGGQPL
                     KHYIGFEISGRIHLGTGLACMAKVKDLVDAGVDCTVFLADWHTWINDKLGGDREVIRR
                     IARDYFEEGLRASFSALGGDPSRLQFVLASDLYASHGRYWETVIEVSKNTTLSRMRRS
                     ITILGRKEGDTVDFAKLIYPAMQVADIFALGVHIAHAGMDQRKAHVIARDTALKLTIA
                     PLCDAEGRRIKPIALHHHLLLGLSKPPVWPPPEAELQDFWASMKMSKSDPSSAVFVDD
                     EPDDIVRKVRKAFCPPAEVQFNPMLDWAESLVFREAGSALTMPAGREGGSVTFDSYED
                     LAEAYAAGRVHPADLKTGIAEWLIAKLEPVRRHFNEPGPRAAREEVTTLLAAASGSR"
     gene            complement(5569..5697)
                     /locus_tag="VNL92_04080"
     CDS             complement(5569..5697)
                     /locus_tag="VNL92_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWO93920.1"
                     /translation="MKGQDESRSELYEQAKRLDIQGRSRMSKEELAEAVHNARARR"
BASE COUNT         1010 a         1833 c         1906 g          976 t
ORIGIN      
        1 gctcgtcgac gggaaccacg agtacaccgt gttcaatcgc gccatcggga cgggcccctt
       61 catcatggaa agctacgaca cgctcggcga tggtggacgg atcaagtacg tccgcaaccc
      121 tgattacttc aagaagggcc agccgttctt cgatgcgatg gaattgccga tcgtcacgag
      181 ctccgagacg atccaggcga tgttcctcca gggcgatacg gacacgagcg gcaccggcct
      241 cgacgcgacg ttaacggcag ccttgaagga agcccggccg gacatgaaga cgttcgaggc
      301 atcgggcttc ttcctgttca aggtgtattg ggacatggcg aaccaggatt cgccatgggc
      361 gcgcgacgtg cggatgcgcc aagccatgtg gtacttcatg ccgtacgacg cgcttctgaa
      421 tcagctcggc gttacgtgct gccgtacggg gccgatgccc cctgggcaga agccctgggc
      481 cttgcccaac gaggatctgc cgggatcggg gctggaccta cagtcgtcga tccaggaggg
      541 cctgaagctg ctcgatgcgg ccggatatcc ggccggcacc gagatcccgc tcgagctcag
      601 catctcgaac ttctacggcg gcgatcttct tggcgaggtg ctgaccgcca tcttcggcgg
      661 gttgaagcag gcttccgggg gagcactgaa catcaatgtc cggttgaacg tcatggagct
      721 cggctcctgg ctcaaggacg tgtatcgcgg cggcggcacg ttcgaggcga cgtcccatgc
      781 cgatgccgtc ttccacgatc cggacaacgg gttctaccgc tattaccact cgaccggagt
      841 tgcgaacaac actcacctca acgatccgaa ggtggacgac ttcctggagc gccagcgccg
      901 cgagttcgat cacgagaagc gcgtcgaggt cgtgcacgag ttccagcgct acctcctcga
      961 catctcgccc acggtatata tcgttgcccc aaactccttc tcgggcgccc agccctgggt
     1021 cgagaactac aagacgcaca ccatcggcta cctcgaaacc ctgcgtcact gggatgagct
     1081 ttggttcaac gagaccgcgc cgcagcgtta gcggggcgac gtcatccaca ggatggatgg
     1141 aaggaggccc agcaccgaat gaaggcagcg attgttcgcc aagtaggagc gaaggtcgaa
     1201 gtcgtcgatg tcgacgtgag ggaccctggt cccggcgagg tgcgcctgcg catcgcggcc
     1261 acgggcgtat gccactcgga cctgtcgttc cagaacggca cgttgccggg aatgcttccg
     1321 gcgatcctgg gtcacgaggg ggcgggcgtc gtcacgcata ccggccccgg cgtgacctca
     1381 gtgaagccgg gggaccacgt catcttgaac tgggtaccgt cgtgcggcgc ctgctacttc
     1441 tgcaaccggg ggcagttcca gctctgcgga tcaccgcgtg gcgcgagcga accgaagctc
     1501 ttcctcgatg gtgtgccgat ccccggcgtg gcggggatgg gtacgtttgc ggaagaaatg
     1561 gtcgtctcgg agaacgcggt cgtgcccatc ccggacgatg tgcccatgga cgtggcggcg
     1621 ctcatcggct gtggcgtgat gaccggcgca ggcgcggcgc tcaatacggc tcaggtcgag
     1681 cctggctcga gcgtcgtcgt gatcggctgc ggcggcgtgg gcatcaacac gattcagggc
     1741 gcgaaggtct gcggcgcggc aaccatcctc gccgtggacc gctacgagaa caagcgcgag
     1801 atggcgaagc ggtttggggc cacccacgcc gccgcgcccg aggagttgcc gcaggcgatg
     1861 caggatctga ccgctggacg cggattcgac tatggcttcg aggtcatcgg cctcgctgcg
     1921 acaatccgcc aagcctggga cgccacgcgc cgcggcggca agacgatcgt ggtcggcgcg
     1981 ggccggttcg acgacatgct gcagctctcc gcgttcgagc tgttcttcat ggagaagcag
     2041 ctcgttggct cggtgtacgg ttcggccaac gtacggcgcg atttcccgcg gctcatcgcc
     2101 ctatggcgca gcggcaagtt ggacctcgag ggcctggtca cgcgccatgt tcagcttggc
     2161 caggtccagg aggcgttcga ggccatggag cggggcgagg tcatccggca ggtcgtcacg
     2221 ttcgagtagc tgttgtcctc aggagaggca gcatcgaggg caaagcgaag agcggatcct
     2281 ccggcggctg gatgccgagc acgcgacgca tccgtgtcgt gtcggcgaca aagcgcgtca
     2341 cctcgatggg gcgtgcgggc aggaccttga gctccgagcg gccgccggtc aggcttcgga
     2401 tacgttcagc gagctcggtg agcgaggttc ccgtgcccga gcccacgttc acgggcccat
     2461 ccaggtcgcg ctcgcccgcg gcgagcaggc attctacggc gatcccgacc cagacgaagt
     2521 cgagaacctg cgagccgccg taaagctcga gtggttcgcc tcgtacggcg cggtcgagcc
     2581 agagagggat gacccgctcg cggtcgcccg ggccgtacac attggcgaat cgcaagatgg
     2641 agcaggttgg cccgccctcc gcctccgaga cgcggcagta cgcttcaccc gcgattttgc
     2701 tggcgccgta agcgttcttg gccccgagcg gcgtgtcctc ggcaacgggg agcgcggccg
     2761 gctcgccgta cgcctcacgt gaggacgaga acaccacgcg ccgtaccccc gccgccctcg
     2821 cggcccgcaa gacgttgtac gtgcccatca cattcgtcgc gaaggcgtaa tctgggtccg
     2881 ccgacgagcc catcacattg gactgagcag cgaggtggaa gacgagctcg gcgccttcca
     2941 tggcagcctg tacggcggct ggatcccgaa tatccccctc gaacaagcgc accgcaggag
     3001 gtatgccctc cggctcgctg cggcgaaagt tgtccaggat cgtcacgtca tcgccgcggg
     3061 cgagcagcgc atctacgagg tggcggccaa gaaagccggc gccgccggtc accagcacgc
     3121 gcgtcacagc cgcgaccgcc cttcggccga catggcaagc ccgagctcag cgcggaagta
     3181 gtcgatcgtg cgcgtcaggc cctcgcgaag accgacgatg ggctcccagc cgaggagtcg
     3241 ccgggccttc gtgatgtccg gttgccggcg ccgtggatcg tcggcgacgg cgtagtcgcg
     3301 gaacacgatc tcgcttcgcg atccggtcag ctcccgcacg agctcggcga attcgaggat
     3361 cgtgtgctcg tctggattgc cgaggttgac gacctcgccc ccagccgcat cgctctccgc
     3421 catggcgatc aggccctcca caagatcgct cacgtaacag agggagcgag tctgcgttcc
     3481 atcgccgtat acggtgatgg gcttgtcgcg gagcgccgcg accacgaagt tcggcaccag
     3541 acggccgtcg tccggtgcgg aacgcgggcc gtacgtgttg aagatgcggg cgatacgaac
     3601 gtcggcgtcg aagcggcgat ggtaagcggc gacgagcgcc tcgccgaagc gcttcgattc
     3661 gtcgtatacc gagcgcggcc ccgttgtgct gacgttgccg cgatacgcct cggtctgcgg
     3721 gtgctccagt gaatccccgt acacttccga agtgctgcag tagacaaacg tggcaccgtt
     3781 gcggacgctg aggtcgagaa ggtggcgcgt gccttcgctg ttcgctcgca tggtctcgat
     3841 ggggaggcgg acgtatcctg gcggactggc ggggctcgcg aggtggtaca cacggtcgac
     3901 gggagggaga ggtggcagcg gctcgatgat atcgtgcgga atgagctcga aggcggcctc
     3961 ccgaaggaag ggctcgacgt tcgacggtcg acccgtggcg aagttgtcga tgcagatgac
     4021 cgaatggccg gctgccagga gccgctccgt gaggttcgag ccgatgaacc cggctccgcc
     4081 ggcgatgacg atgcgcatgt agggacctcg tgtcagggag gtgcgaggag catcctacgg
     4141 acgctcccta tgatcatcgg ccgcttgcgc cgaatcgaca cgccacgtgc gccccggcat
     4201 cgaccgcacc gcgctgttga ctcggactca tacgccacct atgctggaat cgcaatccca
     4261 gcagattccc agggtagcca agcggcctaa ggcagcggtc tgtaaaaccg ccaatcgctg
     4321 gttcgaatcc agcccctggg accaggtcag cctcgcttcc gcgatagtcc gtgagcacgc
     4381 atgacgatcg gctcgcagcc ctccggcctc accgctgacg tagatcagcg gcttgcgctg
     4441 atctcgcgcg gggcggagga gatcatgacg cgcgacgaac tgcgcaagct cctcgccggt
     4501 ggccagcccc tcaagcacta catcgggttc gagatatcgg gccgcattca cctcggcacc
     4561 ggcctcgcat gtatggcgaa agtgaaggac ctcgtcgacg ccggggtcga ctgcacggtc
     4621 tttctcgccg actggcatac gtggataaac gataagctgg ggggcgaccg cgaggtcata
     4681 cgccggatcg cgagggacta cttcgaggag ggattgaggg catcgttctc cgccctcggg
     4741 ggcgacccgt cgcgactcca gttcgtcctt gcgagtgatc tttacgcgag ccacggcagg
     4801 tactgggaaa cggtcatcga ggtcagcaag aacacgacgc tctcccgcat gcgcaggagc
     4861 atcacgattc tcgggcgcaa ggagggcgac acggtcgact tcgccaagct gatctacccc
     4921 gcgatgcaag tcgccgacat cttcgccctg ggggtgcaca tcgctcatgc gggcatggat
     4981 caacggaagg cgcacgtcat cgcgcgcgat actgccctga agctcaccat cgcccccctg
     5041 tgcgacgccg aagggcggcg catcaagccg atcgcgctgc accatcacct tctgctcggc
     5101 ctctcgaaac ctcccgtatg gcccccgccc gaagccgagc tgcaggactt ctgggcgtcg
     5161 atgaagatga gcaagtccga cccgtcgtcg gcggtattcg tggacgatga gcccgacgat
     5221 atcgtgcgca aggtgaggaa ggctttctgt ccgcccgctg aggtgcagtt caacccgatg
     5281 ctcgattggg cggagtcgct cgtcttcagg gaggctggct cggcccttac gatgcccgcg
     5341 ggacgtgagg gcggcagcgt gactttcgac tcatacgaag acttggcgga ggcgtacgcg
     5401 gcgggtcgcg tccacccggc ggatctgaag acggggatag ctgaatggct catcgccaag
     5461 ctcgagcccg ttcgccgtca cttcaacgaa ccgggcccgc gcgcggcgcg cgaggaggtg
     5521 acgaccctcc tcgcagccgc gtcaggatcg cgctaggcct ggatgacctc agcgccgggc
     5581 ccgagcgttg tgaaccgcct cggctagctc ctctttggac atcctactgc gcccctgaat
     5641 atccagccgc tttgcctgct cgtacagctc cgatcggctt tcgtcctgcc ctttcacgcg
     5701 cccccctgcc gtgggtgcac ggggt
//
LOCUS       DATMBB010000186         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_319041, whole genome shotgun sequence.
ACCESSION   DATMBB010000186 DATMBB010000000
VERSION     DATMBB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319041"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VNL92_04085"
     CDS             complement(<1..683)
                     /locus_tag="VNL92_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO93921.1"
                     /translation="MDEVGYWQRQARRRITRRRALSVGAMGAGGAALAAVGCGDDDDD
                     DDGGDSTAAAGGAQRDASQLPSELANARYGGTFILVINDPPIGFDFHATEQPGSQIGA
                     FGVYNGLFRRPEDPPGNFLGLQPELVEDYEQPDETTLVMKFHEGVKWQDVAPVSGRPF
                     TSEDVKYNLERMRGEHPAANQVAGEFRMRDLFQPVESMEASDDRTLTLKTAFPFAPLL
                     DHLSFSWVQ"
     gene            906..2168
                     /locus_tag="VNL92_04090"
     CDS             906..2168
                     /locus_tag="VNL92_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWO93922.1"
                     /translation="MAINTALRLEDIDLTNLDLFVRGDPHPVWTYLRKNAPIYRHPPG
                     EGGVPFWSVTKYMDARQVYSDPVLFSSEDGINLPRGDQLMRREDAADYGGHGQSLIMT
                     DPPKHTKHRQLVNKRFTPRGVAPNEAHIREIVNDILDEVAPRGECDFVIDVAARLPTA
                     VICEMMGIPRQDWDMMFELGNKLIGAQDPEYQGDADVQRTGLQAAMEMTMYFAKIVEE
                     RKRNPGDDLVSALVHGEIDGEKLSDIDLYADCVLFILGGQETTRNATSGGVAAVIERP
                     EERARLLADPSLVPTWVEESLRWTSPITHVRRTATRNTELGGYAIREGDYVVIWIASA
                     NRDEDVFPDPFRFDVGRTPNEHLAFTYGEHFCLGSNLARLELRVMFEEVRRRLLPTME
                     LVGPPERLRSNFVSGIKHMPVRFEPSRA"
     gene            2320..>2850
                     /locus_tag="VNL92_04095"
     CDS             2320..>2850
                     /locus_tag="VNL92_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93923.1"
                     /translation="MLKRLVPAALVLVAMMVPLPSGLANAGSIDVTPSGPYLPGLNGI
                     VVEGSSSAAAVAHTIEAESGLEVDAIWLLSGTWRFYLPDQPEIDGGLNEFSGPVAAAF
                     VVLAAATPVTSEPTGIAEVDAVVEAVLAGNVDALVSMVRYTTVVCTSEPPAAGLPPLC
                     ASVGLPEGSELATLPII"
BASE COUNT          508 a          948 c          907 g          487 t
ORIGIN      
        1 atctgcaccc acgagaacga gaggtgatcg agcaatggag cgaagggaaa cgccgtcttc
       61 aacgtcaagg tccgatcgtc actcgcttcc atcgactcca cgggctggaa caaatcgcgc
      121 attcggaact cgcccgcgac ctggttggca gcagggtgtt cgcctcgcat ccgctcgagg
      181 ttgtacttca cgtcctccga tgtgaagggt cggccgctca caggcgcgac gtcctgccac
      241 ttcacgccct catggaactt catgacaagc gtcgtctcgt cgggctgctc gtagtcctcc
      301 acaagctccg gctggagccc caggaaattc cccggcggat cctcgggacg acggaagagt
      361 ccgttgtaga cgccgaacgc gcctatctgc gagccgggct gctccgtcgc gtggaagtcg
      421 aacccgatgg gcgggtcatt gatgacgagg atgaacgtcc cgccatagcg tgcgttcgcc
      481 agctcagagg gcagttgcga tgcgtcgcgc tgcgctccgc ccgcggccgc cgtcgaatcg
      541 ccgccatcgt catcgtcgtc gtcatcgccg cagccgaccg cggcgagcgc cgcgccaccg
      601 gcgcccatgg ccccaacact cagggcgcga cgacgcgtaa tgcgcctccg cgcctgccgc
      661 tgccaatagc cgacctcatc catggacttt ccccctcttg ctcatgaatt gagcccgccg
      721 ccctcacctc cgtgtcaacg agaggcgcgc gatctgccgt tcgggcatgc taggcttgca
      781 tgacgaggcg cgtcaagcgt cccatcaagc agcgggccat cgttcccgct cgaaggcctt
      841 gacagacggc ggccctactg ccagtcttcc agcaggctta tgcagcattg tcgaggggga
      901 gaatcatggc catcaacacg gctctgcgcc tcgaggacat cgaccttacg aacctcgatc
      961 tcttcgtccg cggcgatcca cacccggtgt ggacgtacct tcggaagaac gcgcccatct
     1021 atcgccaccc acccggcgaa ggcggtgtgc ccttctggtc cgtgaccaag tacatggatg
     1081 cgcgccaggt ctactccgat ccggtgctct tcagctcgga ggacggcatc aacttgccgc
     1141 gaggcgacca gctgatgcgt cgcgaggacg cagcggacta cggcggccac ggacagtcgc
     1201 tgattatgac cgaccccccg aagcacacga agcaccggca gctcgtcaac aagcgattca
     1261 ccccccgggg cgtcgcgccg aacgaggctc acatccgaga gatcgtcaac gacatcctcg
     1321 atgaagtggc cccgcgaggg gagtgcgatt tcgtcatcga tgtggcagca cgcctgccca
     1381 cggcggtcat ctgcgagatg atgggcatcc cacggcaaga ctgggacatg atgttcgagc
     1441 tcgggaacaa gctcatcggc gcccaggacc cggagtacca aggcgatgcc gatgttcaac
     1501 gcacgggcct tcaggcggcg atggaaatga ccatgtactt cgccaagatc gtggaggagc
     1561 ggaagcgcaa cccgggcgac gatctggtga gcgccctcgt ccacggcgag atcgatgggg
     1621 agaagctcag cgacatcgat ctctacgcgg actgcgtgct ctttatcctg ggcggccagg
     1681 agaccacgcg caacgcgaca tccggcggag tcgccgctgt catcgaacgc cccgaggagc
     1741 gcgcgagact actcgccgac ccctcgctcg ttccaacgtg ggtcgaggaa tcactgcggt
     1801 ggaccagccc catcacgcac gtccgtcgca cggcaacgag gaacacggag ctcggcggat
     1861 atgccatccg cgagggcgat tacgtcgtga tctggatcgc ctccgcgaat cgggacgagg
     1921 atgtgttccc cgatccgttc cggttcgacg ttggacgcac cccgaacgag cacctggcct
     1981 tcacttatgg cgagcacttc tgcctcggct cgaacctggc tcgactcgag ttgcgggtga
     2041 tgttcgagga ggtgcgccgg cggctgctcc cgacgatgga gctcgtcggg ccgcccgagc
     2101 ggctccgttc gaacttcgtc tccgggatca agcacatgcc cgtgcggttc gagccaagcc
     2161 gagcgtagat cgcaccccgg cgacggggag ccgcgcgccc gctgtgccta tggcagcgaa
     2221 tacgccaaaa caagcgtgcg ctggtccggt ggcacgcgtt gttggtcccg gttcgtgtgc
     2281 cgtagaaggc ctcgcccggc gttctgaacg agagtcgtaa tgctgaagcg cctcgttccc
     2341 gcagccctcg ttctcgtcgc gatgatggtg ccgctaccgt cgggcctagc gaacgccggc
     2401 agcatcgatg tcacgccgag cggaccgtac ctgccgggtc tcaacggcat tgtcgtggag
     2461 gggagctcct cagccgctgc cgtcgcacac actatcgaag cggagagcgg gcttgaggtc
     2521 gacgcgatct ggctgctgtc cgggacgtgg cggttctacc ttcccgatca gcccgagatc
     2581 gacggcgggc tgaacgagtt cagtgggccg gtagccgcgg ccttcgtcgt gttggccgca
     2641 gcgacacctg tcacgtcaga gccgaccggt atcgcggagg tggacgcggt cgtcgaggcg
     2701 gtgctcgcag gaaatgtgga tgcgctcgtc tcgatggtgc gctacacgac cgtcgtatgc
     2761 acgagcgagc caccggcggc aggcctgccg ccgctgtgcg catcggtcgg cctgccggag
     2821 ggctcggagc tcgccacatt gccgataatc
//
LOCUS       DATMBB010000187         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_513814, whole genome shotgun sequence.
ACCESSION   DATMBB010000187 DATMBB010000000
VERSION     DATMBB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513814"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..633)
                     /locus_tag="VNL92_04100"
     CDS             complement(127..633)
                     /locus_tag="VNL92_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWO93924.1"
                     /translation="MSYKIIETCIGCTACTKRCPTNAITGERNQLHVIDPTLCIDCGA
                     CGLVCPPEAILDTYGEVCKSLKKNEWPKAVVIEEKCIGSGCELCINICPFDALSLQHS
                     DRIDDFFGVSTVDEKKCTGCRLCEDVCGWDAVHIFPLRHEILKPWEALTEHEKELVKQ
                     ARAAEAVR"
     gene            698..1657
                     /locus_tag="VNL92_04105"
     CDS             698..1657
                     /locus_tag="VNL92_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWO93925.1"
                     /translation="MLPDVRLGPTDTMLRPLGLGVWQWGDSYWSYDPTTAYENARAAF
                     TVSLDAGVTLIDTAEAYGKGESERTLGRLIAETGRRPFIATKFQPLPRLRPSSVATAL
                     EGSLERLGVDCIDLYQVHHPFSILRMEHVLGRIADAVRAGKVRHVGVSNYNERQMRKA
                     HRLLARRGIPLVSNQVHYSLLHRAPEANGVLAACRELDITLIAYSPLAQGALTGKYGP
                     GRGKVSGMRRFRRVFRHLGRAMPLVEALEEIGRVHDRTAAQVALNWLAAQPNVIPIPG
                     ARDGRQAEENAHSIDFHLSEAEMALLDGLTRVYRRHGRPIIDM"
     gene            complement(1740..>2438)
                     /locus_tag="VNL92_04110"
     CDS             complement(1740..>2438)
                     /locus_tag="VNL92_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO93926.1"
                     /translation="LGIHAMDASDPNNPQIVHVFMPRDTPGYRIDPSWDTLGMRATRS
                     DDTILEGAFVPDRYVGRVLPAGAADLFVLAIFAWAEPTFASIYLGLAKRALDIGMAGA
                     KKRTSLALSRSMAYHPEVQHLVAESVFALEAAEAQVEKVADDWSNGVDHGGNWPLKLV
                     AAKHNATEAAKKVVDAMMTISGGTGMFKSNELERLYRDVRCGGFHPANSMLAHELVGK
                     IALGIDLGEQPRWG"
BASE COUNT          399 a          932 c          690 g          417 t
ORIGIN      
        1 gccgtgggca cgcctgcgca tccgcgttcc cccgcgccac cccgatgccc gttgagcgca
       61 gcgccacatt ccgcctgcgc ccgcaacggc gaggcggtcc aggaccggga acccgcgtgt
      121 ctctgtctag cgcaccgctt cagccgcgcg cgcctgcttc accagttcct tctcgtgctc
      181 ggtgagcgct tcccacggct tcaggatctc gtggcgcaac gggaagatat ggactgcatc
      241 ccagccacac acgtcctcgc agagccggca gcccgtgcac ttcttctcgt ccaccgtcga
      301 aacgccgaag aagtcgtcga tccggtcgct gtgctgcagg ctcaacgcat cgaacgggca
      361 gatgttgatg cagagctcgc atcccgaacc gatgcacttc tcttcgatga ccaccgcctt
      421 cggccactcg ttcttcttca gcgacttaca gacctcgcca tacgtatcga ggatcgcctc
      481 cggcgggcag accagcccac acgcgccaca atcaatgcag agcgtcgggt cgatgacgtg
      541 cagctgattt cgttcgcccg taatcgcgtt ggtggggcac cgcttggtac acgcagtgca
      601 gccgatgcac gtctcgatga tcttgtagct catgtggcta tcataccaac gtccggacga
      661 aggcgcttcc ggccaccact ccgctcgcga ggtccccatg cttccggatg tccgcctcgg
      721 tccgaccgac accatgcttc gtcccctcgg cctcggcgtc tggcaatggg gcgatagcta
      781 ctggtcctac gaccccacca cggcctacga gaacgcaagg gccgccttca ccgtctcgct
      841 cgatgccggc gtcacgctca tcgatacagc cgaggcctac ggcaaagggg aatcagaacg
      901 caccctcggc cgcctcatcg ccgagaccgg ccgccgccca ttcatcgcga ccaagttcca
      961 gccactgccc cgcctgcgcc cgtcttccgt cgccacggca ctcgaaggaa gcctcgagcg
     1021 cctcggcgtc gactgcatcg acctctacca ggtccaccac ccgttctcca tcctccgcat
     1081 ggaacacgtc ctcggccgca tcgccgatgc cgtcagggct ggcaaggtcc gccacgttgg
     1141 tgtttcgaac tacaacgagc gccagatgcg caaagcccac cgcctgctcg cccgccgcgg
     1201 catcccgctc gtctcgaacc aggtgcacta cagcctcttg catcgcgccc cggaagccaa
     1261 cggcgtcctt gccgcctgtc gagagctgga cataacactg atcgcctata gccccctggc
     1321 ccagggcgcc ctcaccggaa aatatggtcc cggccgcggc aaggtctccg gcatgcgccg
     1381 cttccgccgc gtcttccgcc acctcggccg tgcgatgccc ctggtcgaag cgctcgagga
     1441 gatcggccgc gtccacgatc gcaccgccgc ccaggtcgcc ctcaactggc tcgccgccca
     1501 gcccaacgtc atccccatcc ccggggccag ggatggccga caggccgagg aaaacgccca
     1561 ctccatcgac ttccacctca gcgaagccga gatggccctc ctcgacggcc tcacacgcgt
     1621 ctaccgcagg cacggccgac ccatcatcga catgtgacgc aaacgggccg agggccgaac
     1681 gcgggatgcg ccgctcggcc ctggcgtccc gggctcggcc ctcgcttcgc tcttcgcgcc
     1741 tacccccagc gcggctgttc gcccaggtct atcccgagcg cgatcttccc cacgagctcg
     1801 tgcgccagca tcgagttcgc cggatgaaat ccgccgcagc gtacgtcgcg gtagagccgt
     1861 tcgagctcat tgctcttgaa catccccgtg ccgcccgaga tcgtcatcat cgcgtcgacg
     1921 acctttttcg cagcctcggt cgcattgtgc ttggcggcca cgagcttcaa cggccagttc
     1981 ccgccatggt ccaccccgtt cgaccagtcg tcggcaacct tctcgacctg cgcctccgca
     2041 gcctccaggg caaagaccga ctccgcgacc aggtgctgca cctccgggtg ataggccatg
     2101 ctcctgctca gcgccagcga tgtgcgcttc ttcgcgccag ccatgccgat gtcgagcgcg
     2161 cgcttcgcga ggcccaggta gatgctggcg aacgtcggct ccgcccacgc gaagatcgcc
     2221 agtacgaaca ggtcggcggc gccagcgggc agcacgcggc ccacatatcg gtcggggacg
     2281 aaggcccctt ccaggatcgt gtcatcgctt cgcgtggccc gcatcccgag cgtgtcccag
     2341 ctcgggtcga tgcggtatcc cggcgtgtca cgcggcatga acacgtgcac gatctgcggg
     2401 ttgttcgggt cgctcgcatc catcgcgtgg atgcccag
//
LOCUS       DATMBB010000188         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_294594, whole genome shotgun sequence.
ACCESSION   DATMBB010000188 DATMBB010000000
VERSION     DATMBB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294594"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1599
                     /gene="tig"
                     /locus_tag="VNL92_04115"
     CDS             115..1599
                     /gene="tig"
                     /locus_tag="VNL92_04115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWO93927.1"
                     /translation="MALIIRPDRASRAAQAAAILDDTMKVTSEHIPERQVVLNIEVDQ
                     DQVERSIDQAYRRLVRRTKVPGFRPGKAPRPMLERFLGRDRLMNEALDDLVPEVYRQA
                     IEQEGLQPIDQPDLEITSIEPQISFKATVPLAPTVKLGDYKSVRLRPPEVTVEPSEVD
                     AVLEDLRRRYATIEPRDRPVQDGDIVRADVRAWVDDRQILDEKDAQFAVRAGGFVSLP
                     GFAEALIGRTKGGPHEITIDVAEDYEEPVVAGKRVRYEVDIKEVKEERLPELEDGFAK
                     TVGEGFESLAELRAQIERDLREAHLREAEERYQNDVLAAVADEAEVDFPPVMVEREVE
                     RILHDRFGHGDRREAFEQYLQQVGRSEREVRDEVRPEAAERLIRSLVLSEVSKAEGIE
                     ATDEDIDKEIETVAGAGARSDELRAMFGTVEGRDVLRRSILTRRTLERLAEIARQPDG
                     APAAAPTEDAAAAPASPAKAGRKTRAKAGAAGSGDAGKTSDEGD"
     gene            1649..2257
                     /locus_tag="VNL92_04120"
     CDS             1649..2257
                     /locus_tag="VNL92_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWO93928.1"
                     /translation="MNNRTIQNYMPMVIETSARGERAFDIYSLLLRERIVFLGTPIDD
                     QVANLIIAQLLYLQREDPEKDITMYIHSPGGVISAGLGIYDTMQIIQPDISTVVVGVS
                     ASMATVLLSAGTAGKRYALPNATVHMHQARGGATGEAADIEIAAREILRNQQIIREIL
                     VKHTGQPMERIARDSDRDFYMDAKAAVDYGIVDQILTRDSGA"
     gene            2292..>2627
                     /locus_tag="VNL92_04125"
     CDS             2292..>2627
                     /locus_tag="VNL92_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HWO93929.1"
                     /translation="MANGRASRVQYHCSFCGKNQDQVRRLIAGPGAVYICDECVDLCR
                     EIIDEEATGQTKAKPQPGQRLPTPKSIFDHLNQYVIGQDQAKKVLSVAVYNHYKRIQS
                     STSLDVELEK"
BASE COUNT          547 a          807 c          859 g          414 t
ORIGIN      
        1 acctcagata cgaggacagc atagggatcg cgcgtctggc gccacggcag ctcgtggcgg
       61 ccgtgggccg cgtaccacgc cagaagccgg cgccggctga ttctgcgagt gtccatggcc
      121 ctcatcatac ggcccgatcg cgcttcccgc gccgcgcagg ccgctgctat actggacgac
      181 acgatgaaag tcacctccga gcacattccc gagcgccagg tcgtgctgaa catcgaggtc
      241 gaccaggacc aggtcgaacg ctcgatcgat caagcctata gaaggctcgt gcgacgcacc
      301 aaggtcccgg gctttcgccc cggcaaagcc cccaggccca tgctcgagcg cttcctcggc
      361 cgcgatcgct tgatgaacga ggcgctcgac gatctcgttc ccgaggtgta tcggcaggcg
      421 atcgagcagg agggcctgca acccatcgat cagccggacc tcgagattac aagcatcgag
      481 ccgcagataa gcttcaaggc cacggtgccc ctggcgccga cggtcaaact gggcgactac
      541 aagagtgtcc gtcttcggcc accagaggtg acggtcgaac catcggaggt tgacgcggtc
      601 ctcgaggact tgcgccgccg ttacgcgacc atcgagccgc gcgaccgacc tgtgcaggac
      661 ggggacatcg tccgtgcaga cgtgcgcgct tgggtcgacg accggcagat tctcgacgag
      721 aaggacgcgc aatttgccgt acgagccgga ggcttcgtat cgcttccggg gttcgcggaa
      781 gccctcatcg gccgaacgaa gggtggcccg cacgagatca ccatcgatgt ggcggaggat
      841 tacgaggagc ccgtcgttgc cgggaagcgg gtccgctacg aagtcgacat caaggaagtg
      901 aaagaagagc gcctgcccga gttggaggat gggttcgcga agactgttgg cgaggggttc
      961 gaaagcctcg cggagctgcg tgcccagatt gagcgggatc tgcgcgaggc gcatctgcgc
     1021 gaagccgagg aacgatacca gaacgatgtt ctcgccgcgg tggccgacga ggcggaggtc
     1081 gattttccgc ccgtcatggt ggaacgcgaa gtcgagcgca tcctccacga tcggttcggg
     1141 cacggagatc gccgcgaggc tttcgagcag tacctccagc aagtcgggcg tagcgaacgc
     1201 gaggtgcgcg acgaggttcg ccctgaggca gccgagcgcc tcatccgttc gctggtcctc
     1261 agcgaggtct ccaaggcgga aggcatcgaa gcgacggacg aggatatcga caaggaaatc
     1321 gaaaccgtag cgggcgcggg ggcgcgctcg gatgagctcc gggcaatgtt tgggaccgtg
     1381 gaggggcgcg acgtccttcg gcgtagcatc cttacgcgaa ggacgctcga gcgcctcgcc
     1441 gagatcgcgc gccaacccga tggagcgccg gccgccgcgc cgaccgagga tgcggcagcg
     1501 gcgcctgcct ctcccgcaaa agcagggcgg aagacccgag ccaaggcagg tgcagccggc
     1561 agcggcgatg ctggcaagac gtccgacgaa ggcgattagc gggacggaat ctggcctgcg
     1621 gccaaggtcc gtgagataga aggcaaagat gaataatcga actatccaaa actacatgcc
     1681 gatggtcatc gagacgagcg cccgcggaga gcgcgcgttc gatatttatt cccttttgct
     1741 ccgcgaacgt attgtgttcc tgggcactcc tatcgatgat caagtagcga acctgatcat
     1801 cgcccaactc ctctacctcc agcgtgagga cccggagaag gacatcacga tgtatatcca
     1861 ttcgccgggg ggcgttatct ccgccggcct ggggatttac gatacgatgc agatcattca
     1921 gcccgatatc tcgacggtcg tggtgggcgt atccgccagc atggcgacgg tcctcctgtc
     1981 cgcgggcacg gccggaaaga ggtacgccct acccaacgcc acggtgcaca tgcatcaagc
     2041 ccggggtggt gcgaccggcg aggccgctga catcgagatc gccgctcgcg agattctgcg
     2101 taaccagcag atcatccgtg agatactggt caagcacacc ggccagccca tggaacgcat
     2161 cgcgcgagat tccgatcgtg acttctatat ggacgcgaag gcagcagtag actacggcat
     2221 cgtcgaccag atcctgacgc gcgacagcgg cgcgtagttc ggccggcgga caagcgagaa
     2281 aaggcggtat cgtggccaac ggcagggcga gtagagtcca gtaccactgt tccttctgtg
     2341 gaaagaacca ggaccaggtt cggcgtctga tcgctggccc cggcgccgtc tacatctgcg
     2401 atgagtgcgt ggacctttgc cgcgagatca tcgatgagga ggccaccggc cagacgaagg
     2461 ccaagccgca acccggccaa cggctcccga ccccgaagtc gatcttcgac cacctgaacc
     2521 agtacgtgat cgggcaggac caggcgaaga aggtcctctc cgtcgccgtg tacaaccact
     2581 acaagcgcat ccagagctcc acgagtctgg atgtcgagct cgagaag
//
LOCUS       DATMBB010000189         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_487473, whole genome shotgun sequence.
ACCESSION   DATMBB010000189 DATMBB010000000
VERSION     DATMBB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487473"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1986
                     /locus_tag="VNL92_04130"
     CDS             121..1986
                     /locus_tag="VNL92_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWO93930.1"
                     /translation="MSFGEPIYFALLVTIPLAVLVFAYAGWSRRRAASRFAGMAAGNA
                     RRGRRAVSSVLLLASLVLIAIAAARPQWGEEEATIHREGVDVVVALDTSLSMMAEDVE
                     PNRLGAAKLAIDDLFDALRGDRVGLVVFGGNALVRFPLTTDIDAARHLLQTTVYDEQL
                     GPGSNPASAINAARSAFLDGGTATRVLLLVTDGEQLVAASGEATDVQLEIDRALEEDV
                     RIIVVGVGTEEGGTIPVVDEATGAVTLRLDPATGAPAVSRLDSEALQGLAEAAEGEYV
                     DLSEGEAAPAIADRIAQMEASAFFEADEAHAIERFQIFAGAALGLIALQPLLVRLVPH
                     RARRRRTVAISVTGALLGLLLVACGGDETASLIREGNEHYEGGEYEAALERYREAQVQ
                     RPDLAELNYNAANALFRLGETERAIEEGQRALNTDEPDTLGRAYYSLGNFYASLNRWQ
                     EARAAYRNSLIYEPTDVDAKYNLEVANRQLGSTPPEGQPPTEGEEGEGQGQGEGQPGA
                     SPTPGAEGTATPPPGGTAGPGEGTPADATPGAGQPGGLGDPSGSALPEDTSENPANIS
                     DEERAALREALGQLDPGEAYSVEDALRILEMLRDAREGRITGLPVVPAVPTEPDW"
     gene            complement(1937..2482)
                     /locus_tag="VNL92_04135"
     CDS             complement(1937..2482)
                     /locus_tag="VNL92_04135"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010273642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWO93931.1"
                     /translation="MNLRAIVTNTELGWLAVAFTERGVRASTMPQPHADAAFAALRSL
                     GIDVRLDLSREAEAFATNLRRTVDGEAGAADLPLDLQGTPFQLLVWNAAAAIPRGQTR
                     SYGWIADQIGKPSAARAVGRALGANPAPVVVPCHRVVRENGALGGFGGGLALKQQMLL
                     AEGVELTNRVPLVPPELPVAP"
     gene            complement(2482..>3947)
                     /locus_tag="VNL92_04140"
     CDS             complement(2482..>3947)
                     /locus_tag="VNL92_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWO93932.1"
                     /translation="ADRAADADPSRIVIVDIEARRLLDQLAVPLAPLGVDEDFDATYL
                     EPEQFRVPLGNAESSQDDLPPELEDVLPELRRTYVPPGPGEGAAFQEYGAILPDGYEP
                     KLVEHDTQTLIVERSRSLTDALALEPADDLQRGDHYRVAALSPFATERQLRAASTEYP
                     SDVRDAYLALPDDFPDTVRQLATQLTAGTSSPYDAALAIETYLRSIPYDESIGRPPPD
                     VDRIEYFLFELQRGYFDYHASAMTVMLRAIGVPARLATGYALTEIDESGRYVLRERHT
                     YSWPEVYFPGFGWIAFNPTPNLPTVPRAPGAADAATGVEPVEGGGNFGFDSLDLDALQ
                     DLGPIDVDTPEGGISTDASSNGGGFPWLWLAAGAGAAIAIAGLARLAWERPFAGLPYP
                     AKLWGKTQLLATLAGIGPQPDQTPREFAREAQAKIDGLAQLESLALVYERSRYGIREP
                     NDDERTQLARDYRRVRNRLLARVVRWRWTPKGEEEEM"
BASE COUNT          639 a         1259 c         1398 g          651 t
ORIGIN      
        1 agcgattcct gacgtacgac gagctggcgg tctatgcgct cgcggcagcc gccggcgtgc
       61 tcgttctcga agcgctcctc cgtgcgaccg tattccggcg cgtgccttga ggtggactct
      121 gtgagcttcg gagaacccat ctacttcgct ctcttggtta ccattccgct ggctgtgctc
      181 gtgttcgcgt acgccggctg gagccgcagg cgcgccgcga gccggttcgc cggcatggcc
      241 gcagggaacg cgcgccgcgg gcgccgcgcg gtgagttcgg tgctgctcct cgcgagtctc
      301 gtgctcattg ccatcgccgc tgcccggccc caatgggggg aggaggaggc gacgatccac
      361 cgcgaggggg tggatgtcgt cgttgcgctc gacacgtcgc tgtcgatgat ggcagaggac
      421 gtggagccca atcgcctcgg tgcggcgaag ctcgccatcg acgatctgtt cgacgcgctc
      481 cgtggcgacc gggtgggact cgttgtgttc ggagggaacg ccctcgtccg gttcccgctc
      541 acgaccgata tcgatgcggc gcggcacttg ctgcagacga ccgtctacga cgagcagctc
      601 ggccccggat cgaaccccgc cagcgccatc aatgccgccc gttcggcgtt cctcgatggc
      661 ggtacggcga cacgcgtgct gcttctggtg accgatggcg agcagctggt cgccgcctcc
      721 ggtgaggcga ctgacgtcca actcgagatc gatcgtgccc tggaggaaga cgtgcgcatc
      781 atcgtggtcg gagttggcac tgaggagggc ggcacgattc cggtggtcga tgaggctacg
      841 ggcgcggtga ccctgcgact cgaccccgcc actggtgctc cggccgtctc ccgtctcgat
      901 tcggaggccc tccaggggct cgccgaggcg gccgaaggcg agtacgtcga cctgtcggaa
      961 ggcgaggcag ctcccgccat cgccgatcgc atcgcccaga tggaggcatc ggccttcttc
     1021 gaagcggacg aggcccatgc catcgagcga ttccagatct tcgcgggcgc cgcgctcggt
     1081 ctgatagccc tgcaaccgtt gctcgtgcgg ctggttcccc acagggcacg caggcggcgc
     1141 accgtcgcca taagtgtgac gggcgcattg ctcggcttgc tgctcgtcgc ttgtggggga
     1201 gacgagacgg ccagcctcat ccgcgagggg aacgagcatt acgagggcgg cgaatacgag
     1261 gcagcgttgg agcgttaccg cgaggcacag gtgcagcggc ccgatctcgc cgagctgaac
     1321 tacaacgcgg cgaacgccct cttccggctg ggcgagacgg aacgcgccat cgaggaagga
     1381 cagcgggcgc tcaacaccga tgagccggac acgctaggcc gcgcgtacta tagcctcggc
     1441 aacttctacg cctccctcaa ccgttggcag gaagcgcgtg ccgcctatcg gaattcgctc
     1501 atctatgagc cgaccgatgt cgacgcgaag tacaacctgg aggtcgccaa ccggcaactc
     1561 ggcagcaccc caccggaggg tcagccgcct accgagggcg aggaaggtga ggggcaaggc
     1621 cagggcgaag gtcagcccgg ggcttcgccc acgcctgggg ccgaggggac cgcgacgcct
     1681 ccgcctggtg gtaccgcagg gcctggggaa gggactcccg ctgatgcgac gccgggcgct
     1741 ggtcagccgg gaggcctcgg ggatccgagc ggaagcgcac ttcccgagga cacgagcgag
     1801 aatccggcaa acatcagcga tgaggagcgc gccgccctac gcgaagccct ggggcaactc
     1861 gacccgggcg aggcctacag cgtggaggat gccctgcgca ttctcgagat gcttcgcgat
     1921 gcgcgcgagg ggcgcattac ggggctaccg gtagttccgg cggtaccaac ggaacccgat
     1981 tggtgagctc gacgccctcg gcgaggagca tctgctgctt cagtgcgagc ccacccccga
     2041 atccacccag cgctccattc tcgcgtacga cgcgatggca cgggacgacg acaggggccg
     2101 ggttcgcgcc tagggcgcgg ccgacggcac gagccgccga gggcttgccg atctggtccg
     2161 cgatccagcc gtagcttcgc gtctggccac gtgggatcgc cgccgcggcg ttccacacga
     2221 gcaactgaaa gggcgtgccc tggaggtcga gcgggaggtc ggccgcgccg gcctctccgt
     2281 ccacggtgcg gcgcaggtta gtcgcgaagg cttccgcctc ccgggagagg tccaagcgga
     2341 cgtcgatccc taggcttcga agcgccgcga acgcggcatc tgcgtgaggt tggggcatcg
     2401 tcgatgcgcg tacgcctcgt tcggtgaagg ccaccgccaa ccatccaagc tcggtgttcg
     2461 tgacgatggc gcgcaagttc atcacatctc ctcttcctca cctttcggtg tccaacgcca
     2521 tcgtaccacg cgagcgagca ggcgattccg aacgcgccga tagtctcggg cgagctgcgt
     2581 tcgttcgtcg tcgttcggtt ctcgtatgcc gtagcgggat cgttcgtaga cgagcgcgag
     2641 cgactcgagc tgcgctagtc cgtctatctt tgcttgtgcc tcgcgcgcga actcgcgcgg
     2701 cgtttggtct ggctgcggcc cgatgccggc aagcgtcgcg agcaactgcg tcttgcccca
     2761 gagcttggcg ggatagggca gtccggcgaa ggggcgctcc caggcgagcc ttgcgaggcc
     2821 cgcgattgcg atggctgccc ctgcgcccgc ggccaaccac agccatggga atccgccacc
     2881 gttgctggag gcgtcggtac tgatgccccc ttccggtgta tccacgtcga tcgggccgag
     2941 gtcctgcaac gcatcgaggt cgagtgagtc gaacccgaag ttgccgcccc cctcgaccgg
     3001 ttccacgccg gtcgctgcgt cggcggcacc gggcgcacgc ggcacggtgg ggagattcgg
     3061 ggtcggattg aacgcgatcc agccaaagcc agggaagtac acctcgggcc agctgtacgt
     3121 gtggcgttcg cggagcacgt accgccccga ttcgtcgatc tcggtgaggg cgtagccggt
     3181 ggcgaggcgg gcagggacgc cgatggcgcg aagcatgacg gtcatggccg aggcatggta
     3241 atcgaagtaa cctcgctgga gctcgaagag gaagtactcg atacggtcaa catcgggggg
     3301 tggccggccg atcgactcgt cgtacggaat ggagcgaaga tacgtctcga tggctagtgc
     3361 ggcatcgtaa ggactgctcg tgccagctgt cagctgggtc gcgagctggc gcacggtatc
     3421 cgggaagtcg tccggcaggg ccaagtacgc gtcgcggaca tcgctgggat actcggtgga
     3481 ggcagcgcga agctgacgct ccgtcgcgaa tggcgagagg gccgcaacgc gatagtggtc
     3541 gccccgctgg aggtcatcgg ccggttcaag ggcgagcgca tcggtgagcg agcgtgagcg
     3601 ttcgacgatc agggtttgcg tatcgtgctc gacgagcttg ggctcgtagc catcgggcag
     3661 aatggccccg tattcctgga acgccgcccc ctcgccgggt ccgggcggaa cgtaggttcg
     3721 gcgcagttcg ggcaacacgt cctcgagttc cggcgggagg tcatcctgcg aggattcagc
     3781 gttaccgagt ggtactcgga actgctcggg ctcgaggtag gttgcgtcga agtcctcgtc
     3841 cacgccgagc ggcgcgagtg ggacagcgag ctggtcgaga agcctgcgcg cttcgatatc
     3901 gacgatgacg attcgcgaag gatcagcatc cgcggcgcgg tcggcga
//
LOCUS       DATMBB010000190         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_274198, whole genome shotgun sequence.
ACCESSION   DATMBB010000190 DATMBB010000000
VERSION     DATMBB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274198"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VNL92_04145"
     CDS             <1..1365
                     /locus_tag="VNL92_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWO93933.1"
                     /translation="ELDYERGQAIIDAGIPGVKAVPASRRIYPEGDLASAVLGFVGKD
                     QVGLAGLEADYDDALAGEPGSAVFERDSFGNEIVIGRREVKPPKPGADLVLTIDRYLQ
                     RLVEEELDAALEQHQATGGAIIIQDPTTGAILAMAVRPSFSLLNLDLSDPANEDLYRN
                     RAVTDLYEPGSVFKLVTIAAAIDHGAVTPETPYYDSGLLVIGDSEIRNWNEQGYGTQT
                     IRQVLQNSLNLGSAFAAQRLGAEAFYDYVQQFGFGQLSGIGIGGEAEGHYRTPDMDIW
                     SPVDLATNSFGQGISVTPLQMINAVSAIVNGGELMRPYIVQEVVAEDHREVTQPQVIR
                     RVISEETSRTMRQLMNDVVDGVPGHLAQVAGYSAAGKTGTANIQDETSGQYSSTTIAS
                     FVGAVPADEPRFVMLIKIDGPRDDEFGSTVAAPIFARLAPPVLEYLKVPPEDVPVAGE
                     ETPG"
     gene            1362..>2285
                     /gene="murF"
                     /locus_tag="VNL92_04150"
     CDS             1362..>2285
                     /gene="murF"
                     /locus_tag="VNL92_04150"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWO93934.1"
                     /translation="MSALTQAEVLNALAGELQARSGPTQTEFRRVVVDSRVAERGDLF
                     VALPGERVDGHAFVAEAAERGATGALIEHPVEGVPPGVALFQVPSTLRALQLLAAERR
                     RSAAVRVVAVTGSVGKTTCKELVASVLGTRFRVHKNEANQNNEVGLPLTILAMDRATE
                     VAVLEMGMYARGEIALLASIARPEVGVVTNVGPIHLERLGSIEGIAEAKAELVEALPS
                     DGVAILNGDDRRVAAMAERTEARALLYGEGPACHVRGSDLRTRGLAGITFRISYEGGT
                     TEVATRVPGRVGMMNCLAAAAAGLAFGMPLDE"
BASE COUNT          402 a          780 c          750 g          353 t
ORIGIN      
        1 gagctcgatt atgagcgagg ccaagccatt atcgacgcgg ggataccggg cgtgaaggca
       61 gtgcctgcga gccggcgcat ctatccggaa ggcgacctcg cgagcgcagt gctcggcttc
      121 gtgggcaagg accaagtcgg actggcgggg ctcgaggccg actacgacga tgcactcgcc
      181 ggcgagcccg gcagcgccgt gttcgagcgg gacagcttcg gtaacgagat cgtcatcggc
      241 cggcgcgagg tcaagccgcc caagccgggc gccgaccttg tgctcacgat cgatcgatac
      301 ctccagcgcc tcgtcgaaga ggagctggac gcggcgctcg agcagcacca ggcgaccggt
      361 ggcgccatca tcattcagga ccccacgacc ggcgccattc tcgccatggc cgtgcgcccc
      421 tccttcagcc tgctcaacct cgacctcagc gacccggcga acgaggacct ctaccgcaac
      481 cgagctgtca ccgatctcta cgagccgggg tcggtattca agctcgtgac catcgccgcg
      541 gcgatcgatc acggcgccgt gacgcccgag accccctact acgactctgg cctcctcgtt
      601 atcggcgact ccgagatccg caactggaac gagcaggggt acggcaccca aaccatccgg
      661 caagtgttgc agaacagcct caatctcggc agcgccttcg ctgcgcagcg cctcggcgcc
      721 gaggccttct acgactacgt gcagcagttc ggcttcggtc aactctccgg cattggcatc
      781 ggcggggagg cggaaggcca ctaccgcaca cccgacatgg acatatggtc gcccgtcgac
      841 ctggcgacga acagctttgg gcagggcatc tccgtcacgc cgctccagat gatcaacgcc
      901 gtcagcgcaa ttgtgaacgg cggcgagctc atgcgaccat acattgtgca ggaggtcgtc
      961 gccgaagacc atcgcgaggt tacacagcct caggtgatca ggcgcgtcat ctccgaggag
     1021 acgtcccgca cgatgcggca gctgatgaac gatgtcgtgg atggcgttcc gggccacctt
     1081 gcgcaggtag ccggatactc cgccgccggg aagaccggca ccgccaacat ccaggacgaa
     1141 acgtcgggcc aatactcctc caccaccatc gcttcgttcg tcggcgcggt acccgccgac
     1201 gaacctcgct tcgtcatgct gatcaagatc gatggtccgc gcgacgacga gttcggctcg
     1261 accgtggccg ccccgatctt cgcgcgcctc gccccgcccg tgctcgagta cctcaaggtg
     1321 ccgccggaag acgtgccagt tgctggcgag gagaccccgg gatgagcgcg ctgacgcagg
     1381 ccgaagtcct gaacgcgctc gccggcgagc ttcaggcgcg aagcggacca acgcagacgg
     1441 agtttcgccg ggtcgtggtc gattcgcgcg tcgcggagcg cggcgatctg ttcgtcgcgc
     1501 tgccaggcga gcgggtagac ggacacgcat tcgtggccga ggcggccgaa cgcggcgcca
     1561 cgggcgccct catcgagcat cccgtcgaag gcgtgccgcc cggcgtggcc ctgtttcaag
     1621 tcccgagtac gctccgcgct ctgcaactcc tcgcggccga gcgacgtagg agcgcggccg
     1681 ttcgcgtcgt cgctgtcacg ggcagcgtcg gcaagacgac gtgcaaggag ctcgtcgcct
     1741 ccgtgcttgg cacgcgattc cgcgtgcaca agaacgaagc caaccagaac aatgaggtcg
     1801 gcctacccct caccatcctg gcgatggacc gcgccaccga ggtggccgtg ctggagatgg
     1861 gcatgtacgc gcggggcgag atcgcgctgc ttgcgagtat cgctcgtccc gaggtcgggg
     1921 tggtcaccaa cgttggtccg atccacctcg aacgccttgg ctccatcgag ggcatcgccg
     1981 aggccaaggc cgagctcgtc gaggccctcc cgagcgacgg cgtagccatc ttgaacggcg
     2041 acgaccgtcg tgtggccgcg atggccgaac ggaccgaggc ccgagctctg ctgtacggtg
     2101 aagggccggc ctgccacgtg cgcggttcgg atttgcgaac gcgcggcctt gcaggcatca
     2161 ccttccgcat cagttacgag ggtggcacaa cggaggtcgc aacccgggtt ccaggcagag
     2221 tcggcatgat gaactgcctc gcggcggcgg ccgccggctt ggccttcggc atgccgctcg
     2281 acgag
//
LOCUS       DATMBB010000191         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_89111, whole genome shotgun sequence.
ACCESSION   DATMBB010000191 DATMBB010000000
VERSION     DATMBB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89111"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VNL92_04155"
     CDS             complement(<1..480)
                     /locus_tag="VNL92_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWO93935.1"
                     /translation="MREHAEIVGSGEHYTATGLDNRKLMMWLFLGSECLFFGSLISAY
                     FYYRGRSLTGPYPSDVYDIPFTSVSSFVLLMSSLTMVLALAAIQRNDLRGMRVWLIAT
                     SLLGMVFLGGQVYEFTTFVDEGLTLQQNLFGTTFFVLTGFHGTHVGIGVLILLSIVAG
                     "
     gene            complement(495..2372)
                     /gene="ctaD"
                     /locus_tag="VNL92_04160"
     CDS             complement(495..2372)
                     /gene="ctaD"
                     /locus_tag="VNL92_04160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWO93936.1"
                     /translation="MATASPSVPLIVRRPTSTEGVWGWITTVDHKRIGILYAVTSFIF
                     FLLGGIEALLIRLQLAQPESDLLSADAYNQLFTMHGTTMVFLVVMPMSAAFFNYLIPL
                     MIGARDVAFPRLNALSYWIYLFGGIFINAGFLYGDAPDGGWFGYANLTQEAFLPGDGI
                     DYWALGLQILGISTMMSAFNFAVTIINLRAPGMTMMRLPVFAWMTLVVAFLVITAFPV
                     ITVALFQVSFDRFYEANFYNTAKGGDPVLWQHMFWLFGHPEVYILILPAMGIVSEVLP
                     TFSKKPLFGYPFVIFSGIAIGFMGWGVWAHHMFTVGLGSVAVSAFGISTMLIAVPTGV
                     KIFNWLATMWGGAIRFTPAMLFAIGFIAMFTTGGLTGVTHAIVPADSQQQDTYYIVSH
                     FHQVLFGGAIFALFAGFYYWFPKMTGRMLNDTLGHIHFWGMLIGFNLTFQPMMILGLN
                     GMPRRVFEYPEGMGWDLWNLVATVGAFMIAVSVLAFMINIVRSWRSGERAPADPWDGR
                     TLEWSIPSPPPHYNFAEIPVVHARDPFWHQKYIEDAQGRAIPIPAGGAIDVEEHGEEH
                     ADIHMPSPSYFPVIIALGLPLMAAGLMFGYWITAIGGVILALGVFGMAMEPPDAHGEH
                     A"
     gene            complement(2430..>2695)
                     /locus_tag="VNL92_04165"
     CDS             complement(2430..>2695)
                     /locus_tag="VNL92_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWO93937.1"
                     /translation="PAGAQAATNCAACHTVSGTEAGGRVGPDLTHFASRETLAAGILD
                     QTPENVAKWLRNPADVKPGALMPDLNLSEDEIDALVAYLLSLE"
BASE COUNT          567 a          797 c          900 g          431 t
ORIGIN      
        1 gcccgccaca atggagagca gaatgagcac cccgatgccg acgtgtgttc cgtggaagcc
       61 cgtgagcacg aagaacgtcg tgccgaagag gttctgctgc agggtcaggc cctcgtccac
      121 gaaggtggtg aactcgtaca cctgcccccc gaggaagacc atgccgagga gggacgtcgc
      181 gataagccat acgcgcatgc cgcgcaggtc gttccgctgg atagcggcaa gggcgagcac
      241 catcgtgagg ctgctcatca gcagcacgaa agaagacacc gaggtgaacg ggatgtcgta
      301 gacgtcgctt gggtacggac cagtgagact gcgcccgcga tagtagaagt aggccgagat
      361 gagcgagccg aagaagaggc actccgaacc gaggaagagc cacatcatga gcttccggtt
      421 gtcgagtccg gtcgctgtat agtgctcgcc cgagccgaca atttcggcgt gctcgcgcac
      481 tgtgctctcc tctcctaagc gtgctcgccg tgggcgtcgg gcggctccat cgccattccg
      541 aacaccccga gcgcaaggat gacgccaccg atggcggtga tccagtaccc gaacatgagg
      601 ccggccgcca tgagcgggag ccccagggcg atgatgaccg ggaagtacga aggcgaaggc
      661 atgtggatgt cggcgtgctc ttcgccgtgc tcctccacgt cgatggcccc acccgcgggt
      721 atcggaatgg cgcgaccctg agcatcctcg atgtacttct ggtgccagaa ggggtctcga
      781 gcgtggacca cgggaatctc ggcgaagttg tagtgtggcg gcggcgacgg gatcgaccac
      841 tccagcgtgc gaccatccca cggatcggcc ggcgcgcgct cgcccgagcg ccaggagcgg
      901 acgatgttga tcatgaacgc gagcacagat accgcgatca tgaaggcgcc gaccgtcgcc
      961 acgaggttcc agagatccca acccatgccc tcgggatact cgaagacccg tcgaggcatg
     1021 ccgttcaggc cgaggatcat catcggttgg aacgtgaggt tgaagccgat gagcataccc
     1081 cagaagtgga tgtgtccgag tgtgtcgttc agcatgcggc cggtcatctt cgggaaccag
     1141 tagtagaagc cggcgaagag cgcgaagatc gcccctccga agagcacttg gtggaagtgg
     1201 gagacgatgt agtaggtgtc ctgctgctgg ctatcggcgg gaacgatggc atgcgtgacg
     1261 cccgtgagcc cgcccgtcgt gaacatggcg atgaacccga tggcaaacag catcgccggc
     1321 gtgaaacgaa tggcgccgcc ccacatggtc gccagccagt tgaagatctt gacgccggtc
     1381 ggaacggcga tgagcatcgt ggagatgccg aaggccgaga cggcgaccga gcccagaccc
     1441 accgtgaaca tatgatgcgc ccacacgccc cagcccatga agccgatggc gatgccggaa
     1501 aagatgacga acgggtagcc gaacaacggc ttcttcgaga aagtgggcag cacttcggag
     1561 acgatgccca tggccgggag gatgaggata tacacctccg ggtggccgaa cagccagaac
     1621 atgtgttgcc agaggacggg atcgccgccc ttcgccgtat tgtagaagtt cgcctcatag
     1681 aagcggtcga agctgacctg gaacagcgcc acggtgataa cggggaaggc cgtgatgaca
     1741 aggaaggcta ccaccagcgt catccaggcg aagacgggca gacgcatcat cgtcatgccg
     1801 ggggcccgca gattgatgat cgtgacggcg aagttgaacg cagacatcat cgtcgagatg
     1861 ccgaggatct gcaggcccaa cgcccagtag tcgattccgt cgcccggcag gaacgcctcc
     1921 tgcgtgaggt tcgcgtagcc gaaccagccg ccgtccggag cgtcgccata gaggaagccg
     1981 gcgttgatga agatgccgcc gaacaggtag atccagtagc tgagggcatt gaggcgcggg
     2041 aaggccacgt cgcgcgcacc tatcatcagc ggaatgaggt agttgaagaa cgcagcgctc
     2101 atcggcatca cgacaaggaa caccatcgtc gtgccgtgca tcgtgaacag ctggttgtag
     2161 gcgtccgcgc tcagcagatc gctttccggc tgcgcgagct ggaggcggat gaggagcgcc
     2221 tcgatgccgc ccaacagaaa gaagatgaac gacgttaccg cgtagaggat gccgatccgc
     2281 ttgtgatcga cggtcgtaat ccaaccccac accccctcgg tgctcgttgg gcgacgaacg
     2341 atcagcggta cagaaggcga ggcggtggcc atttcagctt ccctcttcgg ccccgcgagc
     2401 gctggtgact cgcggcgcca tcgatgatcc tactcgagcg atagcagata cgcgacgagc
     2461 gcgtcgatct catcctcact caggttcagg tccggcatga gcgcacccgg cttcacgtcg
     2521 gcggggttgc ggagccattt ggcgacgttc tcgggagttt ggtcgaggat gccggccgcg
     2581 agggtttccc tgctcgcgaa gtgtgtgagg tccggcccga cgcgcccgcc tgcctcggtt
     2641 ccgctcactg tgtggcaagc ggcgcagttc gtggcggcct gggcgccggc cggga
//
LOCUS       DATMBB010000192         3395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_591572, whole genome shotgun sequence.
ACCESSION   DATMBB010000192 DATMBB010000000
VERSION     DATMBB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591572"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VNL92_04170"
     CDS             complement(<1..212)
                     /locus_tag="VNL92_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HWO93938.1"
                     /translation="MRRYLLVPAALLVVAALGLAACGDDDDDADSASAAGTSTSVTGT
                     STSAGAATAEAGDLIAALTVIDGAGF"
     gene            complement(258..665)
                     /locus_tag="VNL92_04175"
     CDS             complement(258..665)
                     /locus_tag="VNL92_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93939.1"
                     /translation="MAEHRHRLDTFAALWRRTDLLALALLAALLPNLFFLGHPLTAES
                     HAESTTGTTHHHAPRGVPGTTEHEEHARHCHAGLATCGDQPVTSGPGQMLLTESLMPQ
                     PPARASLALPAAERSPASFVSRVVTPPPRPSAA"
     gene            784..1644
                     /locus_tag="VNL92_04180"
     CDS             784..1644
                     /locus_tag="VNL92_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWO93940.1"
                     /translation="MPKAIDVHIHPPGPNEDTSERAEAMRRYFRAGAPPKNIDELAEY
                     YRKLDYFGVLFAVDAETATGEPPIPNDYIAECVRKYPDLFVGFGSVDPNKGRMAIKEA
                     ERCVKDLGLRGFKFHAGSQRFFANDARYYPLWEKMQELGTIALFHSGTTGVGAGTPGG
                     GGIKLNYMKPIPYMDDVAADFPGLTIILAHPSFPWQDEQLAMLIHKPNVYMDLSGWSP
                     KYFSPLLVQYAKTIAQDKVMFGSDFPVINPERWMTDFETLGFDDEQVRRKVFFENAKR
                     VLKLEGLQLT"
     gene            1685..2176
                     /locus_tag="VNL92_04185"
     CDS             1685..2176
                     /locus_tag="VNL92_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWO93941.1"
                     /translation="MSEIERMLEANRAYAAQFKKGELPMPPGRQVAVVVCMDARLDPA
                     KFLGLEEGDAHVIRNAGGLVTDDALRSLIISHHLLGTKEFLIIHHTDCGMLTFSNEQA
                     RDLVATTTGADPSDIDFLPFSDLDESVRRDIAKVRESPFIHGETVRGFVYDVRTGQLR
                     EVT"
     gene            complement(2185..2781)
                     /locus_tag="VNL92_04190"
     CDS             complement(2185..2781)
                     /locus_tag="VNL92_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWO93942.1"
                     /translation="MDDYARALVSFFAIIDPIGNLLVFALLSRGLPRQLVVGMAGLSN
                     AAALATISVFVFGGQEVLDYLDISEASFQIAAGVLLLFPAYRLVEHGEPLARLADEGE
                     SAGPYQLALVPLAIPLLAGPAALATAVTFGETSGRLTTLAAAATVLAGTTVLFVVSDA
                     LVRVVGTPALRVAARIVGVLLMAIAADLIVSGLDVVFE"
     gene            2836..>3395
                     /locus_tag="VNL92_04195"
     CDS             2836..>3395
                     /locus_tag="VNL92_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO93943.1"
                     /translation="MPPHPTITLEPVDSVSITTLVDNVTDMLMQDQGPAKRIGLTSEG
                     ERPLVSAAFLEGGATVDQPLAEHGFSALITLTKGGRTRRVLFDAGVTPTGMVDNMRRL
                     SVSPKDVEAIVLSHGHFDHTAGLDGFVREVGRPNLPVIIHPDFWTRRRISIPGRDPFE
                     LPSTSKTALLGAGFDVVEERHPSFLL"
BASE COUNT          622 a         1111 c         1141 g          521 t
ORIGIN      
        1 tggaacccgg cgccgtcgat gaccgtgagc gcggcgatga ggtcgccggc ttcggccgtg
       61 gcggccccgg cgctcgttga cgttcccgtc acgctcgtcg aagttccggc cgcgcttgcg
      121 gagtcggcgt cgtcatcatc gtcaccgcag gcggcgaggc ccagcgcggc caccacgaga
      181 agggcggcgg gcaccagcaa atatcggcgc atggttgcgc tcctttcgtc tgtcgtcgta
      241 tcggagttgg gcggtggtca ggcggcggag ggtcgtggcg gaggcgtgac gactcggctg
      301 acgaacgatg caggcgaccg ctcggcagcc ggcaaagcga gcgatgcgcg agccggcggc
      361 tgcggcatca atgactccgt gaggagcatc tgcccgggcc cggaggtgac cggctgatcg
      421 ccgcacgtgg cgaggccagc atggcaatgc ctggcatgct cctcgtgctc cgtcgtgccg
      481 ggcacgcccc ttggggcatg gtgatgcgtt gtgcccgtgg tcgactctgc gtgggactcg
      541 gccgtgagcg gatggccgag gaagaagagg ttcggtagca gggccgcgag cagcgccagg
      601 gcaaggaggt cggtccgcct ccacagcgcc gcgaacgtgt ccagtcgatg ccgatgttca
      661 gccaaggatc gcctcaccat acagtaaagc acgggcccgg acggggggaa agcagcttga
      721 caggagcacg gccggccgcc tagctttccc aggcgacacc gcgttcagga gggcgaagcg
      781 aacatgccaa aggccatcga cgtccacatc catcctcccg ggcccaacga ggatacgagc
      841 gagcgagccg aggcgatgcg ccgctacttc cgcgccggag cgccgccgaa gaacatcgac
      901 gagctggccg agtactaccg gaaactcgac tacttcgggg tcctgttcgc cgtggatgcc
      961 gagacggcaa ccggcgagcc gcccataccg aacgactaca tcgcggagtg tgtccgcaaa
     1021 taccccgacc tcttcgtcgg cttcggttcc gtcgacccca acaagggccg catggcgatc
     1081 aaggaggccg agcggtgcgt gaaggacctg ggactgcgcg ggttcaagtt ccacgcgggc
     1141 agccagcgct tcttcgccaa cgacgcgcgc tactatccgc tgtgggagaa gatgcaggag
     1201 ctcgggacca tcgcgctctt ccattcgggg accaccggcg tgggcgccgg aacgccgggg
     1261 ggcggtggca tcaaattgaa ctacatgaag ccgatcccgt acatggacga cgtggccgcg
     1321 gacttccccg gcctgacgat catcctggcc cacccctcgt ttccgtggca ggacgagcag
     1381 ctcgccatgc tcatccacaa gcccaatgtg tacatggacc tgagcggatg gtcgcccaag
     1441 tacttctccc cgctcctcgt gcaatacgca aaaacgatcg cgcaggacaa ggtgatgttc
     1501 ggcagcgact ttcccgtcat caatcctgag cgctggatga cggacttcga gacgctgggt
     1561 ttcgatgacg agcaggtccg gcggaaggtc ttcttcgaga acgcgaagcg cgtcctgaaa
     1621 ctggaaggat tgcagctcac gtaggctttg catcctagcg gctagttcat gaggggaggg
     1681 attcatgagt gagatcgaac gaatgctcga ggcgaatcgc gcgtatgcgg cgcagtttaa
     1741 gaagggcgag ttgcccatgc ctcctggccg ccaggtggcg gtcgtcgtct gcatggatgc
     1801 gcgcctcgac ccagcgaagt tccttgggct ggaggaaggc gacgcccacg tgattcggaa
     1861 cgcgggcggt ctcgtcaccg acgacgcgct ccggtcgctg atcatctcgc accacctgct
     1921 cggtacgaag gagttcctca tcattcatca cacggactgc ggcatgctca ccttcagcaa
     1981 cgagcaggcg cgcgacctcg tcgcgacgac gaccggcgcc gacccctccg acatcgactt
     2041 cctcccgttt tccgacctgg acgagagcgt gcggcgcgac atcgcgaagg tccgcgagtc
     2101 accattcatc cacggcgaaa ccgtgcgcgg cttcgtctac gacgtgagga cgggtcagct
     2161 ccgtgaggtg acctgacgcc taccttactc gaacaccacg tcgagcccgc tcacgatgag
     2221 gtcggcggcg atggccatga gcagcacgcc gacgatgcgg gccgcgacgc gcagggccgg
     2281 cgtccccacg acccgaacga gcgcgtccga tacgacgaag agcaccgtgg tgccggcgag
     2341 gacggtcgcg gccgccgcca gcgtcgtcag cctgccgctc gtctcgccaa acgtcacggc
     2401 tgtggcgagc gccgcagggc cggcaagcag cggaatggcg aggggcacga gcgcgagctg
     2461 gtacggaccc gcgctttcac cttcgtctgc gagccgcgcg aggggctcgc cgtgttcgac
     2521 gaggcgatac gccgggaaga ggaggagcac gccggccgct atctgaaagc tcgcctccga
     2581 gatatccagg tagtcgagca cttcctggcc cccgaacacg aacacggaaa tggtggcgag
     2641 tgcggcagcg ttgcttaggc ccgccattcc gacgacgagc tggcgcggta agcctcggct
     2701 cagcagggcg aacacgagca ggttgccaat cgggtcgatg atggcgaaga acgagacgag
     2761 ggcgcgggcg tagtcgtcca tccgccgcat ggtacactga ggccgccctg caagggcgcc
     2821 gtccgaggac gccggatgcc accccacccc accatcaccc tcgagccagt cgacagcgtc
     2881 agcatcacga ctctggtcga caacgtgacc gacatgctca tgcaggacca aggcccggcg
     2941 aagcgcatcg ggctgacgag cgaaggcgag cgaccgctcg tatcggccgc gttcctcgaa
     3001 ggcggcgcca ccgtcgacca gccgctcgcc gagcatggat tttcggcgct catcacgctc
     3061 acgaagggcg gcaggacgcg gcgcgtcctg ttcgacgcgg gagtcacgcc gacgggcatg
     3121 gtggacaaca tgcgccgcct ctcggtctcg cccaaggatg tcgaggcgat cgtgctgagc
     3181 cacggccact tcgatcacac agccgggctg gacggcttcg tccgcgaagt cggccgcccc
     3241 aaccttccgg tcatcatcca tcccgacttc tggacgcggc ggcggatcag catcccgggc
     3301 cgcgatccgt tcgagttgcc atcgacgagc aagacggcgc tcctcggcgc agggttcgac
     3361 gtcgttgagg agcgacaccc gagcttcctg ctcga
//
LOCUS       DATMBB010000193         3027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_358546, whole genome shotgun sequence.
ACCESSION   DATMBB010000193 DATMBB010000000
VERSION     DATMBB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358546"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..401)
                     /locus_tag="VNL92_04200"
     CDS             complement(144..401)
                     /locus_tag="VNL92_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWO93944.1"
                     /translation="MCRNIQPLFNFDPPATDEEIRAAALQFVRKVSGFTKPSRANEAA
                     FARAVDEVAAASRRLVDSLTTAAPPRDRAVEAARARARRAR"
     gene            complement(446..742)
                     /locus_tag="VNL92_04205"
     CDS             complement(446..742)
                     /locus_tag="VNL92_04205"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWO93945.1"
                     /translation="MMVTIFRSRLRPEHEAEYEREATRIAQIAATMPGFVGIKTFEAD
                     DGERVSIVEFESAEAHESWRLHPEHRAAQRLGRERFYSEYHIQVGEVSKDYRWP"
     gene            complement(801..1628)
                     /locus_tag="VNL92_04210"
     CDS             complement(801..1628)
                     /locus_tag="VNL92_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWO93946.1"
                     /translation="MLEAGLLLLLGIAVGAFGTLVGAGGGFVLVPILLLVYPDRDAAT
                     VTAMSLFVVFANATSGSLAYARQRRIDYRSAAWFALGTLPGALAGAIVVGYIPRRAFD
                     FLFAAVVAVVAVYLSLPRGAQEVRTPVAGRGVVTRTLTDSAGHTFRYAYRLWQGVLIS
                     SAVGFMSSLLGIGGGIVHVPVMILLLHFPVHIATATSHLVLALMSFEGSVVHFVQGEL
                     GWDETFLQTIAIAAGAIPGAQIGARLAQRIHERMIVRGLASALLLISIRLWVAALVG"
     gene            complement(1718..1876)
                     /locus_tag="VNL92_04215"
                     /pseudo
     CDS             complement(1718..1876)
                     /locus_tag="VNL92_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562757.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2134..2619)
                     /locus_tag="VNL92_04220"
     CDS             complement(2134..2619)
                     /locus_tag="VNL92_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HWO93947.1"
                     /translation="MAAADGAGYVLSKDEGTATWFLDTLMVVKASGEQTGGSFTLIEQ
                     VMPAGYGPPLHIHALDDEAFYVLDGEITFICGDRSLRASAGSFVFLPRGVKHAFSVEA
                     SAPARVVQITSPSGFERFVAEAGEPARALTLPPPAPPDLGGLIAAAAKYGIEILESPG
                     P"
     gene            2715..>3027
                     /locus_tag="VNL92_04225"
     CDS             2715..>3027
                     /locus_tag="VNL92_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWO93948.1"
                     /translation="MAQEPPNRRMLGALLRLPFQAIVAAIHRSLTDAGYVDLGPSHFI
                     VFQHLPAEGARLTDLARAAQITKQSMGYLVDHLEERGYVKRVPDPEDGRARLVQLTDK
                     GY"
BASE COUNT          546 a         1015 c          994 g          472 t
ORIGIN      
        1 cgttcggttc gcagggcgcg cgtgtcctca gcatggccat cccgcccatg gatcgcagcg
       61 accagcacgt catcgccgag ccgccggccg aataggcgac ggacggggcg aggggcggca
      121 tccaccccat cgcgccgccc cagctaacgc gctcgcctcg ccctggcccg tgccgcctcg
      181 acagcacgat cgcgcggggg agcggccgtc gtgaggctgt cgacgagacg ccgcgaggcc
      241 gcggccacct catcgacggc tcgcgcgaag gcggcctcgt tcgcgcgcga cggcttcgtg
      301 aagccactca ccttgcgcac gaactggagg gcggccgccc ggatctcctc gtccgtagcg
      361 ggcgggtcga agttgaagag cggttggatg ttgcgacaca cgggatagct cctggactcg
      421 ccggcgcctt cttcgagcgg cgcccttagg gccagcgata gtccttgctg acctcgccga
      481 cctggatgtg gtactcggaa tagaagcgct cgcgtcccag tcgctgggct gcgcgatgct
      541 cgggatggag gcgccaggac tcgtgcgcct ccgctgactc gaactccacg atcgagacac
      601 gctcgccgtc gtccgcctcg aacgtcttga tgcccacgaa gccaggcatg gtcgcagcga
      661 tctgtgcgat gcgagttgct tcccgctcgt actccgcctc gtgctcgggt cgaagacgcg
      721 agcggaagat ggtgaccatc attgcaagcc tccgcgggca aggtatcgac caatgataca
      781 ggctctgcac tggttcgccg ctagcccacg agtgccgcca cccacagccg gatcgagatg
      841 aggagcagcg ccgaagccag ccctcggacg atcatccgtt catggatgcg ctgggcgagc
      901 cgggcaccga tctgcgcccc cgggatggcg ccagcggcga tggcgatcgt ttgcaggaac
      961 gtctcgtccc acccgagctc gccctgcacg aagtggacca cgctgccctc gaacgacatc
     1021 agggcgagga cgagatggct ggtggcagtg gcgatgtgaa caggaaagtg gagcagcagg
     1081 atcatcacgg gcacatgcac aatcccgccg ccgatgccga ggaggctcga catgaacccc
     1141 acggcgctgc tgatgaggac tccctgccaa agccgatagg cgtaccggaa cgtgtggccg
     1201 gcgctgtcgg tcagcgtgcg cgtcacgacg cctcgcccag cgacgggtgt gcgcacctcc
     1261 tgcgcccccc gcggcaagga gaggtacacc gcgaccaccg cgacgaccgc ggcgaacagg
     1321 aagtcgaagg cgcgccgcgg gatgtagccc accacgatcg cgccggccag cgcgccgggg
     1381 agcgtgccca gcgcgaacca cgccgcactg cgatagtcga tgcgccgctg gcgagcgtag
     1441 gcaagcgatc ccgaagtcgc gttggcgaag acgacgaaga gtgacatagc cgtaacggtg
     1501 gccgcatcgc gatcggggta gacgaggagc aggatgggaa cgaggacgaa gccgccgccg
     1561 gcgccgacca gcgtgccgaa cgcccctacg gcgatgccga gcaagagcag gagtcctgcc
     1621 tcaagcaagg tcgctcgcca tcttcctggc ccagcactgc gattcgccga agcggaggct
     1681 tagggtcggc gccgttccgc accgacgcgg caggccgtca tcgctccgaa tttcgcggct
     1741 cagcgggccc ttctccttcg agcggaaggc ggatggtgaa ggtggcgccg tgcccggcct
     1801 cgctctcgag cgcaatcgat cctccgtgcg cctccactat gcccttggcg atggctaagc
     1861 ccaagcccgt gccgccggtc tcgcgcgcgt gccttgtccg cgcgatcgaa ccggtcgacg
     1921 acatgcggta ggctaccggc aggctttgct gacaggaggc tgtggtgggg agatggtcta
     1981 gcgcccctgg ggacgttccg gccaagggtg cacggtcata acgcgcggtg accggctcag
     2041 ctgttagcgc gccggctcac cctacctgag aagtgcaagc gtggcgaagg cacaatcgat
     2101 cgatgaacat tgctccgaag agccttcccc ctgtcaggga cccggcgact ccagaatctc
     2161 gatgccgtac ttggccgcgg cagcaatcag ccctcccaag tccggggggg cgggcggtgg
     2221 cagcgtcaat gcgcgggcgg gttcgccggc ctccgcgacg aaccgctcga acccggatgg
     2281 cgaggtgatc tgcaccaccc gcgccggcgc gctcgcttcc acgctgaagg cgtgcttcac
     2341 accacgaggt aagaacacaa atgacccggc actagctcgg aggctacgat cgccgcaaat
     2401 gaacgtgatc tcgccgtcga gaacgtagaa ggcttcatcg tcaagagcgt ggatgtggag
     2461 tgggggccca tacccggccg gcatcacctg ctcgatgagc gtgaagctac cgcctgtctg
     2521 ctcgcccgat gccttcacga ccatgagcgt atcgaggaac cacgtcgccg tcccttcgtc
     2581 ctttgagagt acgtacccgg cgccatcagc cgccgccatt tgcgacctcc cgcttgtgtt
     2641 agattatcgt cagatagtct gacgacctcc aaaagtatga ccctctgact aggttgtcaa
     2701 gagagcaata gccgatggcg caggaaccac caaaccgccg catgctcgga gcgttgttgc
     2761 gactcccttt ccaggccatc gtggcggcga tccaccggtc gctgaccgac gctggctatg
     2821 tcgacctcgg cccttctcac ttcatcgttt tccagcactt gccggccgag ggcgcgcgcc
     2881 tgactgactt ggcccgagcg gcacagatta cgaagcagtc catgggatac ctcgtggacc
     2941 atttggaaga gcgcggctat gtgaaacgcg tccccgaccc cgaagatggc agggcgcgcc
     3001 tcgtgcagct gacggacaag ggctacg
//
LOCUS       DATMBB010000194         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_589845, whole genome shotgun sequence.
ACCESSION   DATMBB010000194 DATMBB010000000
VERSION     DATMBB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589845"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /locus_tag="VNL92_04230"
     CDS             complement(<1..1739)
                     /locus_tag="VNL92_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO93949.1"
                     /translation="MRNLSIALAYVTRYRWHYLAGFVAVVAASFVVMAPPFVLGRAID
                     DLRDGLSGEGGPSQARLAAYAGIILAFAAIESVLRWGYRLLVSGTSRRVEYDIRNDLA
                     AHLTTLDQRFFLRSQTGDLMARCTNDLQMVRDLLGPTLIDLIRIVTMLIVGFVLLLNV
                     NVKLGLIAYAYFPVVALLIASCSTIVEARYRAVQDQFGTLSTRVQENVSGQRTIKAYA
                     QEQTEIEGFAAANREMQRRSMAWAYWTAALWPLMVIGTGASTVLVLWFGGREVVSGNM
                     TLGQFVEFNGYLGILTWPLMSLGWSATAFQTGIASWNRIAEVLKTEPSIATAPAAAAP
                     ARIRGDVSFRDVTFGYGDEPVLRHVNLDIPAGTTVALVGATGAGKTTLLNLLARLWDP
                     WEGSVAIDGIDLRQLPLPVVRQAVAFVPQETFLFSDSLVENIGYGRANASPEALQFAL
                     ETSQLVNDLPQLTHGIETVIGERGVTLSGGQKQRTAIARAVIKDAPILVLDDALSHVD
                     THTEEEILRRLRAVMAGRTTILVAHRTSTVAAADRIVVLERGRIAESGTHEELLARNG
                     LYARFYRRQLLAE"
     gene            complement(1888..>2108)
                     /locus_tag="VNL92_04235"
     CDS             complement(1888..>2108)
                     /locus_tag="VNL92_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWO93950.1"
                     /translation="ERMRRLRLCARLDAIVHAEFGDDAQRRREAVRRRRASDPGFMLC
                     QAAVMIGPEKLPAEAFDERQEAELAAMR"
BASE COUNT          376 a          636 c          774 g          322 t
ORIGIN      
        1 tgctcggcca ggagctggcg ccgatagaag cgcgcgtaga gcccgtttcg cgcgagcagc
       61 tcttcgtggg tgcccgattc ggcgatgcgg ccccgttcaa ggacaacgat ccggtccgcg
      121 gctgcgacgg tggatgtgcg atgggcgacg aggatggtcg tgcggccggc catgacggcc
      181 cggagccgcc ggaggatctc ctcctctgtg tgggtgtcga cgtgggagag cgcatcgtcg
      241 aggacgagga tgggggcgtc cttgatgacg gcgcgggcga tggcagtccg ctgcttttgg
      301 ccaccggaga gggtgacacc ccgttcaccg atgacggtct caatgccgtg ggtgagctgt
      361 ggcaggtcgt tcacgagctg actggtttcg agcgcgaact ggagcgcctc gggcgaagca
      421 ttcgcccgcc catagccgat gttctcgacg agcgagtccg agaagagaaa cgtctcctgg
      481 ggcacgaagg ccacggcctg gcgcacgacg ggaagcggca gctggcgaag gtcaatgccg
      541 tcgatggcga ccgagccctc ccaggggtcc cagagccgtg cgagcaggtt gagtagcgtg
      601 gttttcccgg cgcccgtggc gccgaccagc gcaacggtcg tgccggccgg gatatcgagg
      661 ttcacgtggc ggaggaccgg ctcatcgcca tagccaaagg tgacgtcgcg aaaactgacg
      721 tcgccgcgga tgcgggccgg ggcggccgcc gccggcgccg tcgcgatcga tggttcggtc
      781 ttcaacacct cggcgatacg gttccaggag gcgatgccgg tctgaaaggc ggtggcactc
      841 cagccgaggc tcatgagggg ccacgtgagg atgccaaggt agccgttgaa ttcgacgaac
      901 tgtccgagcg tcatgttgcc agaaacgact tcgcgacccc cgaaccagag gacgaggacg
      961 gtggaggcac cggtgccgat gaccatgagc ggccagaggg cagcggtcca gtaggcccag
     1021 gccatcgagc ggcgctgcat ttcgcggttg gcggcagcaa agccctcgat ctcggtctgc
     1081 tcctgcgcat aggccttgat ggtccgttgg ccggagacgt tctcctgcac gcgggtggag
     1141 agcgtcccga actggtcctg cacggcccgg tagcgcgctt cgacgatcgt gctgcagctc
     1201 gcgatgagca gcgccacgac agggaagtac gcgtaggcga tgaggccgag cttcacgttc
     1261 acgttgagca gcaatacgaa gccgacgatg agcatcgtga cgatgcggat caggtcgatc
     1321 agcgtcgggc cgaggaggtc gcgcaccatc tggaggtcgt tggtgcagcg ggccatgagg
     1381 tcgcccgtct gggagcgcag gaaaaagcgc tggtcgagcg tcgtgaggtg cgcggcgagg
     1441 tcgttgcgga tgtcgtattc gacacggcgc gaggtgccag agacgaggag gcgatatccc
     1501 cagcgaagca cgctttcgat ggcggcaaac gcgaggatga tgccggcata ggccgcgagg
     1561 cgagcctgcg agggaccgcc ctcgccactg agcccatcac gcaggtcgtc gatcgccctg
     1621 ccgaggacga acggcggcgc catgacgacg aaggaggccg ccaccacggc aacgaacccg
     1681 gcgaggtagt gccagcggta gcgggtaacg taggcgaggg cgatggacag gttccgcacg
     1741 ggtcaccgcg gggcacgccg gtggacggac acggcgaacg ttctgtgccg gagagcgtgc
     1801 cgactccaca agaaacgcat tcgaaacacg aaggttaaag gcggcatgtg cttcctctat
     1861 ggtccgcgtc acagaagcgg cgaagcttta ccgcatcgcc gccagctccg cttcctggcg
     1921 ttcgtcgaac gcctcggccg ggagcttttc tggcccgatc atgaccgcgg cctggcagag
     1981 catgaagccc gggtcgctgg cgcgccgtcg gcggacggct tcacgccggc gctgggcgtc
     2041 gtccccgaac tcagcatgga cgatcgcgtc cagccgggcg cagagacgca ggcgtcgcat
     2101 ccgctcgc
//
LOCUS       DATMBB010000195         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_564499, whole genome shotgun sequence.
ACCESSION   DATMBB010000195 DATMBB010000000
VERSION     DATMBB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564499"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VNL92_04240"
     CDS             complement(<1..251)
                     /locus_tag="VNL92_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93951.1"
                     /translation="MNDPHLESLTYTLVPGADTSYRDEAEPVTIETDDFTARLERGAL
                     NVTMKDHHSSEDSARAIVDPYLRAWETVEALESGRPEFT"
     gene            complement(458..1228)
                     /locus_tag="VNL92_04245"
     CDS             complement(458..1228)
                     /locus_tag="VNL92_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93952.1"
                     /translation="MTLLIANVVPQGLIFAADRNLSVNGIKVAEETKVVKVGEVVAGY
                     AGRARVGGMPMCDWLRDFLRRRSGASLAELGPALAAELELAMGAVPREHRGTIVHVGG
                     FERASDGRPLPVIWYVRDAEIQTDGSVAFLPKFEARDELKVAGAEPPYFGRATGDDIR
                     RRLNESGTALPWAGFRQGWDLSVFGVLDQLGWIFSAELIRGIAREHTHTAPTTLEEWE
                     RFVRFSILVYGAYFEAFYPDGQRPVGGGVDVATVAWPE"
     gene            complement(1240..1500)
                     /locus_tag="VNL92_04250"
     CDS             complement(1240..1500)
                     /locus_tag="VNL92_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93953.1"
                     /translation="MEKSEEDRLTIQACRSEARALGYTLLVDEVPNLGELTHAAFAAP
                     YIPGQPSMGRFLAYGRSAVEAAELGADLLRRIVERNEPWPSK"
     gene            1666..2799
                     /locus_tag="VNL92_04255"
     CDS             1666..2799
                     /locus_tag="VNL92_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93954.1"
                     /translation="MSRYTVVLKSASQLRLMAKAIGRPGSWLLNREPPDTTEVRVLDD
                     VLEENEIPWHRGITFEVTAEAPTIDDAVEAARGEAEATGILMATAARAPIGPARPVLA
                     VDVTPGIEERSFLQWVYGGGIVIGKTVVTPEAFGAFHGALLEQGSPGTTDRRLLERAL
                     LSLSWYRLALGETDALTRFMHLWLAVEALEPLLADHFGCDSQGFGGLRALAEAQQPGE
                     HGEGSKRVSAALGVRRALFHSLRANVQELKERAREQIEFAESLLHRGWGEILGISDLD
                     KRLPTGSVTAFDAYILIRATIVEQDETFGGRLPYMVSELEPYRVNPDDPDHLNVSYRG
                     KLSLRNADLARLHRLEIRGPRGPNVGQFVELGDETVSGDKDSS"
BASE COUNT          553 a         1016 c          989 g          515 t
ORIGIN      
        1 aacgtgaact ccggtcggcc gctctccagc gcctcgacgg tttcccacgc gcgcaagtac
       61 gggtcgacga tcgcccgcgc cgagtcctcc gaagagtggt ggtccttcat cgtcacgttc
      121 aatgctcccc gctcaagccg cgcggtgaag tcgtccgttt cgatcgtcac cggttccgcc
      181 tcatcgcggt acgaggtgtc cgcgcccgga acgagggtgt acgtcagcga ttcgaggtgg
      241 ggatcgttca cgctgcgccc gccttccaag acctttccac gtcccaagtc gctttccgtc
      301 cgcggcgccg gtcgctgaaa ggaaagaccg ccgtccgggg aagttgtgaa tcggccgtac
      361 caagcattat gcgaagcgcg agatgctcgt cgtcgctggc gccctctccc tccgttgggc
      421 tcgtgccggt gcgcgcagta cgcgttcgcc caccgcccta ctcaggccac gccacggttg
      481 ccacgtcgac accgcccccg accggacgtt gcccatctgg atagaacgcc tcgaagtacg
      541 ccccgtacac gaggatcgag aaacgtacga agcgttccca ctcctccagg gtcgtcggtg
      601 cggtgtgcgt gtgctcgcgc gcgataccgc gaattagctc ggcggagaag atccagccca
      661 gctggtcgag aactccgaac accgaaaggt cccagccctg ccggaaaccg gcccacggca
      721 gggccgtccc gctctcgttc agcctgcgcc gaatgtcgtc gcctgtcgcc cgcccgaagt
      781 acggcggctc agcacctgcg accttcagct cgtcccgcgc ttcgaacttc ggcagaaacg
      841 cgacgctccc atcagtttgt atttccgcgt cgcggacgta ccagatgacc ggcaacggac
      901 ggccatcgct cgcgcgctcg aagccgccga catgaacgat cgtcccgcgg tgctcccgcg
      961 ggacggcgcc cattgccagc tccaactcgg ccgcgagcgc tggcccgagc tcagccaggg
     1021 acgcgccgct gcggcggcgc agaaagtcgc gaagccagtc gcacatcggc attccaccca
     1081 cacgcgcgcg cccggcgtat cccgcgacaa cctcgccgac tttcaccacc ttcgtctcct
     1141 cagccacctt gatcccgttg acactcaggt tgcgatccgc cgcaaatatc agcccctgcg
     1201 ggacgacgtt cgcgatcagc agcgtcacgc gctagagctc tacttagacg gccaaggctc
     1261 gttgcgctcg acgatccggc gaaggagatc agcgccgagt tcagccgcct cgactgcgga
     1321 gcgcccgtaa gccaggaatc gtcccatcga cggctgcccc gggatgtacg gcgcggcgaa
     1381 ggccgcatgg gtgagctcgc cgagattcgg cacctcgtca acgagcagcg tgtagccgag
     1441 tgcgcgagcc tcactacgac acgcctggat tgtcaggcgg tcctcctccg acttctccat
     1501 gcgtccaggc taggccgcgg atcagacggt ccaccgtgga aacccgcatc agcgcaaggg
     1561 agcgaacaac ggtcgatcgt cgggcggtcg tagtcgacca ccgaatcgcc ggcgatagcg
     1621 tctgaccgtg accggcgccg ctcaaccgcc actagtctcg acagggtgag tcgctacacg
     1681 gtcgttctca agtcggcgtc ccagctccgg ctcatggcga aggcgatcgg tcggcctggt
     1741 tcatggctcc tcaataggga gccccctgac acgaccgagg tgcgagtact cgatgacgta
     1801 ctcgaggaga acgagatccc gtggcaccga ggaattacgt tcgaggtgac ggcggaggct
     1861 ccaacgatcg atgacgccgt tgaggccgct cggggagagg cagaggcgac tggaatcctg
     1921 atggcgactg cggcgagggc cccgattggt ccggcgcggc cggtgttggc agtcgacgtg
     1981 acccccggaa ttgaggagcg ctcgttcctt cagtgggtct acggtggcgg gatagttatc
     2041 gggaagaccg tcgtgacgcc agaggcgttc ggggccttcc acggcgccct gctcgaacaa
     2101 gggagccccg gaaccacaga tcgtcggcta cttgagcgcg ctcttctgtc ccttagctgg
     2161 tatcgacttg ctctcggtga gacggatgcg ctgacgcgat tcatgcatct gtggcttgct
     2221 gtcgaagcgc tagaacctct tctcgcagat cacttcggct gcgattcgca gggcttcggc
     2281 gggctccgtg cgcttgcgga ggctcagcag cccggcgagc acggcgaggg ttcaaagcgc
     2341 gtgtcagcgg cgctcggagt gcgccgagcc ctgtttcatt cgctgcgtgc gaacgtccag
     2401 gagctcaaag agcgggcgcg cgaacagatc gagttcgccg agagcttgct tcatcgtgga
     2461 tggggcgaga ttctcggaat cagcgacctc gacaagcgcc taccgacagg ttcggtgacc
     2521 gcgttcgacg cctacatcct gatccgagca accattgtcg agcaggacga gacgtttggg
     2581 ggcaggctgc cgtacatggt cagcgaactc gagccgtacc gagtcaaccc ggacgacccg
     2641 gaccatctca acgtgagcta tcggggaaag ctgtcgctac gcaatgctga cctcgctcgg
     2701 ctgcaccggc tcgaaatacg cgggccgcga ggaccgaatg ttggccagtt cgtcgagctc
     2761 ggcgacgaaa cggtctccgg cgacaaagac tcgagctgac tgtggcagtt gcgcgagcca
     2821 agtgagcggc agcggagcgt cgcgccgcgt aacagcggcc gaccggcggt atcgtcaaac
     2881 gatgcgctgg agaccgtgga agtggcggcg cgagcgccgg aagctcgaac cgctcggcgt
     2941 cattcccgaa cacgagagcg cggttcgtcg cctgatggca gacgcccgcg gcggaacgta
     3001 cgaggccttg tgtcactcga ggaggcgaaa caggtagacg acgcggcggt cgtgatggaa
     3061 ggcgactacg gcg
//
LOCUS       DATMBB010000196         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_82018, whole genome shotgun sequence.
ACCESSION   DATMBB010000196 DATMBB010000000
VERSION     DATMBB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82018"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VNL92_04260"
     CDS             <1..329
                     /locus_tag="VNL92_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158066129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWO93955.1"
                     /translation="THLPTGIVVTCQNERSQGRNRESAMKVLHARLLELELERRAEEQ
                     ARMKGEHVSAGWGNQIRSYVLHPYKLVKDHRTGYETSDTTAVLDGDLDAFLAAYHKSE
                     LGVAAS"
     gene            329..1717
                     /locus_tag="VNL92_04265"
     CDS             329..1717
                     /locus_tag="VNL92_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HWO93956.1"
                     /translation="MLDGAVAARILRPLWRAALLALLVPLIMLLGASLVDAQSAPSIS
                     SSQASVQFPEGIRFNLEAQAPGEIERVQLRYTILPDGVPTNTVADCAPPSGATISCSV
                     LVRGNDPPRIYIPPFTELRYSWEVVPTEGPTARSEEQSVVYTDTRFEWAQQSEANLTI
                     YSYGNTDAAALLSAGRAALDRTSSLLATPVSFPVKVVAYASREDMAAALQNRSATFGE
                     LVVTLGVRVSSDTVIVLNDEGAVATLQHELAHVVTKQAGEGTLGGLPAWLDEGTAVYA
                     QDNVDFGYLTAYENAVESDSLLSLRAMSSPTGNPENVNLFYGQSYAFVRYLVDTYGEE
                     RFAQLFATFKAGARVDEALQDVYGKDLLELENEWRAALGLPPRSVEEARAEAERTATF
                     TPLGAGGIALPSTPDAADGEDDAGRPVESNDDDDDDGVDGATVAVIAVAAVAALGATA
                     GGGVALYRYRRR"
     gene            complement(1714..2316)
                     /locus_tag="VNL92_04270"
     CDS             complement(1714..2316)
                     /locus_tag="VNL92_04270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93957.1"
                     /translation="MAKTEHRWFAYMWENRLSKQEAMRPWREYTAGRARGRVLEVGIG
                     NGASIPYYRGIDELIGVEPDPAMRRYAEPRAKEAPFPVRLVAGEAQHLPLADDSVDVV
                     VSNLVYCTIPDVDAALGEVARVLKPGGEFRFFEHVRASGNIAGRLQDAVTPLWSWFGA
                     GCHPNRRIEDAIRRAGFDIVEIERFRAGLPHIAGLARPPA"
     gene            complement(2349..>2691)
                     /locus_tag="VNL92_04275"
     CDS             complement(2349..>2691)
                     /locus_tag="VNL92_04275"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWO93958.1"
                     /translation="GMREADVLLVNPVIDGMNLVSKEGVLVNRRDAVLVLSETAGSFE
                     QLGAHALPVAPADVEGTVRALHAALTMPKEERHARAEALREAVRNDDIIVWLQRQLSD
                     LRAVAGSTATV"
BASE COUNT          450 a          899 c          914 g          428 t
ORIGIN      
        1 tcacccatct accgactggc attgtagtta catgtcagaa cgagcgatcg caggggcgca
       61 accgcgagag cgccatgaag gtgcttcacg cgcggctcct ggaactggag ctggagcgcc
      121 gcgcggagga gcaggcgcgc atgaagggcg agcacgtgag cgccggatgg ggcaaccaaa
      181 tcagaagcta cgtgctccac ccctacaagc tcgtgaagga ccaccgaacg ggatacgaga
      241 cgagcgacac cacggcggtg ctggacggcg acctcgacgc gttcctggcc gcctatcaca
      301 agtcggaact gggcgtggcg gcctcgtgat gctggacggc gcagtcgcag cccggatcct
      361 gcggccactc tggcgagcgg cgcttctggc gctgctcgtc ccgctgatca tgctgctcgg
      421 cgcgtccctg gtcgatgcgc agtcggcccc ctcgatcagc tcgtcgcagg cgagcgtcca
      481 gtttccagag gggatccgct tcaacctgga ggcgcaggcc ccgggcgaga tcgagcgggt
      541 ccagctacgc tatacgattc ttccggacgg cgtaccgacc aacaccgtcg cggattgcgc
      601 acctccgagc ggcgcaacca tctcgtgctc ggtgctcgtt cgcgggaacg atcctccccg
      661 catctacatc ccgcccttca cggagctacg gtactcgtgg gaggtggtgc ccactgaagg
      721 tcccacggca cgctccgaag agcagtcggt cgtctatacc gacacccggt tcgaatgggc
      781 ccagcagagc gaggcgaacc taacgatcta tagctacggc aacacggacg cagcggcgct
      841 gctttcggcg gggcgcgctg cgctcgacag gacgagttcg ctgttggcca cgccggtgtc
      901 ctttccggtg aaggtggtcg cgtatgcgag ccgcgaggac atggcggccg ctttgcagaa
      961 ccggagcgcg acgttcggcg agctcgtcgt gaccctgggc gtgcgcgtgt cctccgatac
     1021 cgtcatcgtg ctcaacgacg aaggcgcggt cgcgacgctc cagcatgagc tggcccacgt
     1081 cgtcacgaag caggcgggcg agggcacgct cggcggcctg ccggcgtggc tcgacgaggg
     1141 aacagcggtc tacgctcagg acaacgtcga cttcggctac ctcacggctt acgagaacgc
     1201 ggtcgaaagc gactccctgc tgtcccttcg cgccatgtca tcgcccacgg gcaatcccga
     1261 aaacgtcaat cttttctatg ggcagtcgta cgcgttcgtg cgctatctcg tcgatacgta
     1321 cggcgaggag cgcttcgcgc aactcttcgc gacgttcaag gccggcgcgc gtgtcgacga
     1381 ggcgctgcaa gacgtgtacg gaaaggacct cctcgagctc gagaacgaat ggcgcgcggc
     1441 cctcgggctc ccgccgcgct ccgtcgaaga agcgcgcgcc gaggcggagc gtacggccac
     1501 gttcacgccg cttggggccg gcggcatcgc gctgccgtcc acaccggacg ccgcggatgg
     1561 cgaagatgac gccggccgtc ctgtcgaatc gaacgatgac gatgatgacg acggcgtgga
     1621 tggagcgacg gtggccgtca tcgcggtcgc tgcggtcgct gctctcggcg caaccgctgg
     1681 gggaggcgtt gccctctacc gatatcggcg ccgctaggcc ggaggcctcg ccaggccggc
     1741 gatgtgcggc aagcccgcgc gaaagcgctc gatctcgacg atgtcgaagc ccgcgcggcg
     1801 gattgcatcc tcgatgcgac gattcgggtg gcagcccgct ccaaaccagg accacagcgg
     1861 cgtcaccgca tcttgcaggc gcccagcgat attgcccgag gcgcgcacgt gctcgaagaa
     1921 ccggaactcg ccgcccggct tgagaacgcg agccacttcg ccgagcgcgg catcgacgtc
     1981 ggggatcgtg cagtagacga gattcgagac gacgacatcg accgagtcgt cggcgagcgg
     2041 gaggtgctgc gcctcgccgg ccaccaggcg cacagggaag ggcgcctcct tggcacgcgg
     2101 ctcggcgtag cgtcgcatcg cggggtccgg ctcgacaccg atgagctcgt cgatccccct
     2161 gtagtacgga atgctcgccc cgttgccaat gccgacctcg agaacgcgtc cgcgagcgcg
     2221 gccggccgtg tactcccgcc agggccgcat cgcctcctgc ttgctcaggc ggttctccca
     2281 catgtaggcg aaccatcggt gctcggtctt cgccattttt tgctcccctc ccagctccgc
     2341 ggggttcatc agacggtggc tgtcgagccc gcgaccgccc ggagatcgct gagttgccgc
     2401 tgcagccaca cgatgatgtc gtcgttgcgc acggcttcgc ggagggcctc agcacgggcg
     2461 tggcgttcct ccttgggcat cgtgagggcg gcgtgcaggg ctcgcacggt gccctcgaca
     2521 tcggcaggag cgacggggag cgcatgggcg cccagttgct cgaaggagcc cgcggtttcg
     2581 gagaggacca ggacggcgtc gcggcggttg acgagcaccc cctccttgga cacgaggttc
     2641 atgccgtcga tcacgggatt gacgagcagc acatcggcct cgcgcatgcc g
//
LOCUS       DATMBB010000197         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_398074, whole genome shotgun sequence.
ACCESSION   DATMBB010000197 DATMBB010000000
VERSION     DATMBB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398074"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VNL92_04280"
     CDS             complement(<1..289)
                     /locus_tag="VNL92_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911835.1"
                     /note="catalyzes the interconversion of 3-phosphoglycerate
                     and 2-phosphoglycerate; this enzyme does not require the
                     cofactor 2,3-bisphosphoglycerate as a phosphate donor;
                     BPG-independent PGAM; aPGAM; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HWO93959.1"
                     /translation="MDLTLAREIAVRDGDNLGKIILLVADGLGGLPHPDTGRSELETA
                     HTPNLDALARESMCGLTIPVAPGVTPGSGPGHLGLFGYDPVRYNIGRGVLEA"
     gene            complement(312..1505)
                     /locus_tag="VNL92_04285"
     CDS             complement(312..1505)
                     /locus_tag="VNL92_04285"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWO93960.1"
                     /translation="MRKKTVRDLDVRERRVFLRADLNVPLDESGRVADDTRIRAIVPT
                     VRYLSDQRARIIIASHLGRPRGVDESLRLAPLRGPLESILGVPMTYVPDCIGPEVQRA
                     VEQLRPASALLLENLRFHPEEEANDQEFARKLASLADVYLNDAFGAAHRAHASTEAIA
                     HLLPSGAGLLMERELTFLGRVVTSPERPYAAVIGGAKVSSKLGVLQALLDKVDLMIIG
                     GGMANTFMRAEGFDMGGSRIEEDLVQEARAIIEQAERRKVRLLLPTDLVVADAFDADA
                     RTQIVGVKDVPAGWQALDIGPKTVEVYSRSLEGCRTVVWNGPMGVFEMPLFAQGSLAV
                     GKAIAELTDCTTIVGGGETAQLAAMLQEHYPGLQFSHISTGGGATLEFLEGRQLPGVA
                     ALMDA"
     gene            complement(1534..>2077)
                     /locus_tag="VNL92_04290"
     CDS             complement(1534..>2077)
                     /locus_tag="VNL92_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWO93961.1"
                     /translation="SRWQYITSKVVAISGMVVMGTLAGMLVALIVSALITLIAGNPDY
                     SFVDVGYIGDSFASYGRTLLAMSPYIAMAVFFSTWGRSTMGGIGASLGVLFLETIITS
                     LMQLAGEVPARITNVFPGANADTLMLENGLQIAIQQRQDPTLDVPVLDSPFAALVLAT
                     YVVGFLIATYVLFERRDVQP"
BASE COUNT          383 a          697 c          651 g          346 t
ORIGIN      
        1 ccgcctcgag cacgcctcga ccgatgttgt aacgaaccgg gtcgtacccg aacaggccga
       61 gatgcccggg accgcttccc ggcgtgaccc caggggccac cggtatcgtg agtccgcaca
      121 tcgattcgcg cgccagcgcg tcgaggttcg gcgtgtgggc cgtctcgagt tccgaacggc
      181 ccgtatctgg gtgcggtagg ccgccgaggc cgtcggcgac aagcaggatg atctttccga
      241 ggttgtcccc gtctcgaact gctatctctc tggcaagcgt gagatccatg gatcgtaagt
      301 ctcggctccg gtcaggcgtc catcaaggcg gcgacgcccg gaagctgacg gccctcgagg
      361 aattcgagcg tcgcaccccc acccgtcgag atgtgcgaaa actgcagccc cggatagtgc
      421 tcctggagca tcgccgccag ctgcgcggtc tctcctccgc cgacaatggt cgtgcagtcg
      481 gtcaattcgg caatcgcttt gcccaccgcg agggaaccct gcgcaaagag cggcatctcg
      541 aacacgccca tcggtccgtt ccataccacg gtccggcatc cctcgagcga tcggctgtag
      601 acctccaccg tcttcgggcc gatgtcgagc gcctgccatc cagctggcac gtccttcact
      661 cctacaatct gggtacgcgc gtccgcatcg aaggcgtccg cgacgacaag gtccgtcggc
      721 aggagcaagc gaaccttgcg tcgctctgcc tgctcgatga tggcccgagc ctcctggacc
      781 aggtcctcct cgatgcgcga gcctcccata tcgaacccct cggctcgcat gaaggtattc
      841 gccattcccc cgccgatgat cataaggtcg accttgtcga gcagggcctg cagcacgccg
      901 agcttggagc tgacctttgc gccgccaatc accgccgcat agggccgctc cggcgatgtg
      961 acaacacgcc ccaggaaggt cagctcgcgt tccatcagga gtccggcgcc gctcggcaaa
     1021 aggtgcgcga tcgcctcggt cgaagcgtgc gcacgatggg cggcgccgaa ggcatcgttc
     1081 aggtagacgt cggcaagtga ggcgagctta cgcgcgaact cctggtcgtt cgcctcctcc
     1141 tccgggtgaa accgcaggtt ctcgagcaag agcgcgctag ccggacgcag ctgctcgacg
     1201 gcgcgctgaa cctcaggccc gatgcagtcc ggcacatagg tcataggcac gccaaggatg
     1261 ctctccagcg gcccgcgaag cggggcgagc cgcagcgatt cgtcgacgcc gcgtgggcgc
     1321 ccgaggtgcg aggctatgat gatgcgcgcc ctctggtcgc tcagatagcg aaccgtgggg
     1381 acgatggcac ggatgcgcgt gtcatcggcc acgcgtccgg actcatccaa cggcacgttg
     1441 aggtcggcgc gcaagaagac acgtctctcg cgcacgtcga ggtcgcgcac cgtcttcttc
     1501 cgcatcgcct atcgagcata ccatgaccgt gattcagggc tgcacgtcgc gccgctcgaa
     1561 tagcacatag gtcgcgatga ggaagccgac gacgtacgtg gccagcacca gcgccgcgaa
     1621 gggcgaatcg agcacaggga cgtcgagggt cgggtcctgc cgttgctgga tagcgatctg
     1681 gaggccgttc tccagcatca aggtgtccgc gttcgcgccg gggaagacgt tcgtgatgcg
     1741 ggccggcacc tcacctgcga gctgcatcaa actcgtgata atcgtctcca ggaacaagac
     1801 gccgaggctc gccccgatgc cgcccatcgt cgaacgtccc catgtggaga agaagacggc
     1861 catcgcgatg tacggcgaca tcgcgagcaa cgtccgcccg taggaggcga atgagtcgcc
     1921 gatgtagccc acatcaacga agctgtagtc ggggttgccc gctatcagcg taatcagcgc
     1981 cgacacgatg agcgcgacca gcatgccggc cagcgtaccc atgacgacca tcccggaaat
     2041 ggcgacgacc ttgctcgtga tgtactgcca tcggctc
//
LOCUS       DATMBB010000198         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_326942, whole genome shotgun sequence.
ACCESSION   DATMBB010000198 DATMBB010000000
VERSION     DATMBB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326942"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VNL92_04295"
     CDS             complement(<1..836)
                     /locus_tag="VNL92_04295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWO93962.1"
                     /translation="MFTLRGLISALVALLTIALAGAAAACGGEASGADRVSVSTATAT
                     PGEPPPKLTLTATAVRQGGVLGMQVEGAGIAVDTIQFGDRLLRLAPTPEGAWAAIGVA
                     VDEPPGEYALEVSVVGPDARTHVLHAVVQVTPYDFPIDAVDLAPDVASLLDPSRVAEE
                     ERILQRTLRGFSDAPAPPVRFQRPLAGEITTTFGQARVYNGQEPAAADHHRGMDIGAD
                     EGTEVAAAAAGTVVLARELPIRGTYVMIDHGLGVFTGYGHLAEMRVREGDPVTAGQVI
                     GLV"
     gene            1055..1351
                     /locus_tag="VNL92_04300"
     CDS             1055..1351
                     /locus_tag="VNL92_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93963.1"
                     /translation="MNSSLIGKIEKAKRYATSERHRIRVEELRVTFDGVNDVHEVILR
                     DAAWQCSCEFFAMWRVCAHTMALERILEDMLPAEARSGELALGSAAMNEAGAPR"
     gene            complement(1429..1599)
                     /locus_tag="VNL92_04305"
     CDS             complement(1429..1599)
                     /locus_tag="VNL92_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWO93964.1"
                     /translation="MEWQPGMRVIDLLHREGWQGDDAAAIAAIVNGEQAAAERELHDG
                     DQVELMVAIAGG"
     gene            complement(1681..3561)
                     /locus_tag="VNL92_04310"
     CDS             complement(1681..3561)
                     /locus_tag="VNL92_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWO93965.1"
                     /translation="MDEKPYNGKILNVNLSTGEISTEHLDDSFYRTYLGGYGMGSRLL
                     FDRIPAGADALGPDNILGFFPGLLTGTPLFGSRFQVCAKSPKNNGWGDANCGGDFGPN
                     LKFAGWDGILFYGKAEKPVYLYIEDDKVELRDASDIWGMMAIECEDQLKAEFGKKSSI
                     ALIGPAGEKLSRMAGVCNERGRLAARSGLGGVMGSKMLKAVVVKPSRQIIMGTNKELR
                     QTLRSSLDGFVKPLADFFRTYGTTGITPMSAMNGDAPVKNWGGVGIADFQQGTLMTGD
                     NVNARMEKRYACWSCPLACGGESKPSENPAYNYPHHTHRPEYETMTAFGTMALMNDVD
                     ALIMANHLCNEYGLDTIAAGATVTFAIECFENGILTKEDTGGLELHWSDAQAIIQLLH
                     LIGRREGIGDVLGDGVKFAADRIGRGSEQFAMHIDGEEVPMHDPKLNPGYGTTYKLDP
                     TPARHTQGTEGGGEAGGPRGVPLPEFKGDRTVYSGRGPLHKGVAAYQHVVNGSGFCMF
                     VTSAAESGRIPEWINTATGWDTNWDELLKTGDRIGNLRMAFAVKHGNYPPQRHVPGRI
                     IGVPPQEAGPHQGITVDLDTLSREFLEAAGWDQQTGRPSEATLRALGLDDVAQQLAAI
                     PA"
     gene            complement(3655..>3909)
                     /locus_tag="VNL92_04315"
     CDS             complement(3655..>3909)
                     /locus_tag="VNL92_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93966.1"
                     /translation="REAAQFLAGLADRRPAMRTPLLQASKAYREEALALSRLTSLFPY
                     PHGGDIGNPRAREQGARYLASAYMNEGEAISSLREALGSA"
BASE COUNT          599 a         1269 c         1329 g          712 t
ORIGIN      
        1 accaggccga tgacctgccc cgccgtgacc ggatctccct cgcggacccg catctccgcg
       61 aggtgtccgt acccggtaaa aacgccgagc ccgtggtcga tcatgacgta cgtgcccctg
      121 atcggcaact cgcgggcaag caccacggtg cccgcggccg cggcggcgac ctcagttcct
      181 tcgtcggccc cgatatccat gccgcgatgg tggtccgcgg cggcgggctc ctgaccgttg
      241 tagacacgcg cctggccgaa ggtcgtcgtg atctcgccgg cgagggggcg ctggaatcgg
      301 accggcggcg ccggcgcgtc gctgaagccg cgcaacgtgc gttggaggat gcgctcctcc
      361 tcggcgacgc ggctcggatc gaggagcgac gcgacgtccg gggccaggtc gacggcgtcg
      421 atcgggaagt cgtacggagt cacctggacg acggcgtgga ggacgtgggt tcgtgcgtct
      481 gggccgacca cgctgacctc gagggcgtac tcgccgggag gttcgtcgac cgcgacgccg
      541 atggccgccc acgctccctc aggcgtgggg gctagccgga ggaggcggtc gccgaattgg
      601 atcgtgtcta cggcgatgcc cgcgccctcg acctgcatgc ccagcacgcc tccctgacgc
      661 acggctgtcg cggttagcgt gagcttcggt ggcggctcgc caggggtcgc ggttgcggtc
      721 gaaacggaga cacggtcggc gcccgatgcc tcgcctccgc aagcagccgc agcgccggcc
      781 agagcgatcg tgaggagcgc gacgagggcg gatatcaggc cgcgaagggt aaacacggcc
      841 ctattgtaga ggcgctcgtt ggccgaagat agccttggca gggttgcgga ctgtggctgc
      901 tctgaccctt gacgggtgcg gaggcgcttg tctacactac ggcccaaccg aggggatgat
      961 gatcatgagt ctccgagtac gtaacaagcg agaagaggcg ggcccagccc tgggggcccg
     1021 ccttgattgt ttctgaaact ggaggagcga gcggatgaac tcgagtctca tcggcaagat
     1081 cgagaaggcc aagcggtacg ccacgtcaga gcggcatcgt atccgtgtcg aggaactcag
     1141 ggtgaccttc gacggtgtga acgacgtgca cgaggtcatc cttcgcgatg ctgcctggca
     1201 gtgctcatgc gagttcttcg cgatgtggcg ggtgtgcgct cacacgatgg cgctggagcg
     1261 cattctcgag gacatgttgc cagccgaggc gcgcagtggt gagctggccc tgggatcggc
     1321 agccatgaac gaagccgggg ctccacgcta gaatccgcct tccgcggtac aggtgacgag
     1381 agggcgtgct tagcacgccc tctcggtttc actgcagcgg gggtcgggtt agcccccggc
     1441 gatagccacc atcagctcga cctggtcgcc gtcgtgcaat tcgcgctcgg cggccgcctg
     1501 ctcgccgttg acgatggcag caatggcggc agcatcatcc ccttgccacc cctcgcggtg
     1561 cagcaggtcg atgacgcgca tgccaggctg ccactccata tcgaactccg gcttccttgt
     1621 ggccgatgac ctcgccagcg tcggaaagag ccgaacatgg agcgccacgc ggaatctccg
     1681 tcaggccgga atcgccgcga gttgctgggc cacgtcatcg agtccgagcg cccgcagcgt
     1741 ggcctcgctg ggccgtcccg tctgctggtc ccagccggcg gcctcgagga actctcgcga
     1801 gagcgtgtcg aggtcgacgg tgatgccctg gtggggcccc gcctcctgcg gggggacgcc
     1861 gatgatgcga cccggcacat ggcgctgcgg cgggtagttg ccatgcttga cggcgaaggc
     1921 catacggagg ttaccgatgc ggtcgccggt cttgaggagc tcgtcccaat tggtgtccca
     1981 tcccgtcgcc gtgttgatcc actccgggat gcgtccggac tcggcggccg acgtgacgaa
     2041 catgcagaac ccgctcccgt tcacgacgtg ctggtaggca gcgacgccct tgtggagcgg
     2101 accgcgtccg ctgtagacgg tgcggtcgcc cttgaactcc ggcagcggca cgcctcgcgg
     2161 accgccggcc tcgccgccgc cctcggtgcc ctgcgtgtgc cgggccggtg tcgggtcgag
     2221 cttgtaggtc gtgccgtagc ccgggttcag cttcgggtcg tgcatcggca cttcctcgcc
     2281 gtcgatgtgc atcgcgaatt gctccgaacc tcgaccgatc cggtccgcgg cgaacttgac
     2341 gccgtcgccg aggacatcgc cgatgccctc gcgccgcccg atgaggtgga ggagctggat
     2401 gatggcctga gcatcgctcc aatgcaactc gagcccgccc gtgtcttcct tggtcaggat
     2461 tccgttctcg aagcactcga tggcgaaggt cacggtcgcg ccggcggcta tcgtgtccaa
     2521 cccgtactca ttgcagaggt ggttggccat gatgagcgcg tcgacgtcgt tcatcagagc
     2581 catggtgccg aacgcggtca tcgtctcgta ctcgggccgg tgcgtgtggt gcggatagtt
     2641 gtacgccggg ttctccgatg gcttggattc gccgccgcag gcgagcgggc agctccagca
     2701 ggcgtagcgc ttctccatcc gggcgttgac gttgtcgccc gtcatcagcg ttccctgctg
     2761 gaagtcagcg ataccgacgc cgccccagtt cttgaccggc gcgtcgccgt tcatggccga
     2821 catcggcgtg atgccggtcg tcccgtacgt gcggaagaag tccgcgagcg gcttcacgaa
     2881 cccgtcgagc gagctccgca gcgtttggcg tagctctttg ttcgttccca tgatgatctg
     2941 ccgcgacggc ttcacaacga cggccttcag catcttggag cccatcacgc cgccgagccc
     3001 ggagcgtgct gccagccggc cgcgctcgtt gcagacccct gccatgcgcg agagcttctc
     3061 gccggctggg ccgatgaggg cgatactcga cttcttgccg aactccgcct tgagctggtc
     3121 ctcgcattcg atggccatca tgccccaaat gtcgctggca tcccgcaatt cgaccttgtc
     3181 gtcttcgatg tagaggtaca ccggcttctc ggccttcccg tagaagagga tgccgtccca
     3241 gccggcaaat ttgagattcg ggccgaaatc gccgccgcag ttggcgtcgc cccagccatt
     3301 gttcttcggc gacttggcgc agacctggaa gcgcgagccg aacagcggcg tgccggtgag
     3361 cagcccgggg aagaatccca ggatgttgtc cgggcccagc gcatcggcgc ccgcagggat
     3421 tcgatcgaag agcagccgag agcccattcc gtagcccccg aggtaggttc ggtagaacga
     3481 gtcgtcgagg tgctcggtcg aaatctcgcc cgtgctgagg ttcacgttca ggatcttgcc
     3541 gttgtagggt ttctcgtcca tgggctcgtt gtcccccttc tcacgttttg actctcgatt
     3601 gctcgcatat tctacacgat gttcctcgcc tcgcaaccgt atggcagttc gagttcaggc
     3661 ggatcccaat gcctcgcgta gcgaggagat cgcctcgcct tcattcatgt aggcggacgc
     3721 gaggtagcgc gccccctgct cgcgcgcccg cgggttcccg atgtcgccgc catgagggta
     3781 tgggaagagc gaggtgaggc gcgacagagc gagcgcctcc tcgcggtagg ccttgctggc
     3841 ctgtaggagg ggcgtccgca tggcgggccg ccggtccgcg aggcctgcca ggaactgggc
     3901 agcctccct
//
LOCUS       DATMBB010000199         2676 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_336100, whole genome shotgun sequence.
ACCESSION   DATMBB010000199 DATMBB010000000
VERSION     DATMBB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336100"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..>2676
                     /locus_tag="VNL92_04320"
     CDS             96..>2676
                     /locus_tag="VNL92_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93967.1"
                     /translation="MATVRDLATELRCTVSDIQRAARAVGVAAKSPGYPLKTDDCHRI
                     REHMQAQARAAAAAQAAAAAEAKRREEAASSSPILEERRVGVRGSVGPVAGMTAARLL
                     LHEDFVYWLAADQTDGRLKKRANLVLRQLAAFGRTSIVKSVRGVAKGWRRSPLGGNQG
                     RQFYLWWAPTGAPPVAELPLAKDTVLIRAVRHHDETAVALAPGNPSSDYIDLNPADLV
                     NRPDEYGYAFNDAQVSIAKSTSAVRFIKGHPGAGKTTALWLAASLAEGHRALYLTCNA
                     KLALDAEQYFRALGPETLAAEVMTFEQLTAHLLQTGDSKPLPTLDVDEAAGEFEEVIA
                     GNYRGILGPWDDCIDELYAELHAHAVGECVPIAIRDVPASEGLVLPKAVYLNLRAGVL
                     GEQGARTAAQIAMQLAESGLIEQLFPGPARARRALDRLMADSSAVKRFDQVDLVFVDE
                     AQDLTLVELFLLVRLCAAIGSFRQGAPPAFVAAGDEGQTVRPSDFEWGQLADLTASQI
                     GRRSEFELTGNVRSPQNIALVVNRSWDMYRHFAKDERPRGYASAEIEEASAGRVIYTT
                     CRTPEDLREIIEVFDGIPNAALVYPGYRVPDSYRSVASATSDLVLTSRSAKGLDFQTV
                     GVLDAGHQMARLKELALDAGPDSRLSALWGRILADHLRVALSRATENLVLIDVEPGDE
                     ARAEVETLCADAQLTEMEPEELVTFLRRQDESDAAELALEFCEEAEKLLGNQSVRAYR
                     RSRQAVVLLGDAKSPNAVQDAALRRQAWTLRGVAAAELLRHRAGRQKVPYSADELLTD
                     AVSSLRRAERGADAQAIQLINRFVTGSAGPHSLVGEVKELLPLLPSLERHIERYAREV
                     IVSW"
BASE COUNT          467 a          858 c          918 g          433 t
ORIGIN      
        1 gccgtcgtag tcggcatgag gtcgcgaagc accgccacct tcgctgctcg cgtgcaacga
       61 cacgcggatg agtagtgatg agtaagtaac cgaccatggc gacggtcagg gatctcgcga
      121 ccgaattgcg atgcacggtg agcgacatcc aacgcgccgc tcgcgccgtc ggcgttgccg
      181 cgaagtcgcc tggctacccg ctgaagacgg atgactgcca ccgcatccgc gagcacatgc
      241 aggcccaggc tcgcgcggcg gctgccgcgc aggcggctgc cgcggcagag gcgaagcgtc
      301 gagaggaagc agcctcgtcg tctcccattc ttgaggagcg tcgtgttggc gttcgtgggt
      361 cggtcgggcc cgtcgccggc atgacggcgg cacggctcct tctgcacgag gacttcgtct
      421 actggctcgc ggccgaccag accgacggac ggctcaagaa gcgggcgaac ctcgtcctgc
      481 gccagctcgc ggcatttggc aggacgtcca tcgtgaagtc ggtgcgggga gtcgccaagg
      541 gttggcggcg atcaccgctg ggcggcaacc aggggaggca gttctatctc tggtgggcgc
      601 ccacgggagc gccccccgtc gccgagttgc cgctggcgaa ggacacggtc ttgatcagag
      661 cggtccgcca tcacgacgag acggcggtgg cgctggcgcc gggtaaccca tcgtcagact
      721 acatcgatct gaatccggcg gacctggtca acaggcccga cgagtacggg tatgcgttta
      781 acgacgctca ggtttcgatc gcgaagtcca cgagcgccgt tcgcttcatc aagggacatc
      841 cgggcgccgg caagacgacg gcgctatggc ttgcggcctc cctcgccgag ggacatcgag
      901 ccctgtacct cacatgcaat gcgaagttgg cactggatgc ggagcagtac ttccgagcgc
      961 tgggccccga gacgcttgct gccgaggtca tgaccttcga acagctcacg gctcaccttc
     1021 ttcagaccgg cgattcgaag ccgctgccta ccttggacgt cgatgaggcc gccggagaat
     1081 tcgaggaggt catcgccggg aactaccgag ggatcctcgg gccctgggac gactgcatcg
     1141 acgagctcta cgccgagctc cacgcgcatg ccgtcggcga gtgcgtgccg atcgcaatcc
     1201 gcgacgtacc ggcgagcgaa gggctcgtgc tgccgaaagc ggtctacttg aacctgcgag
     1261 ccggtgtact cggcgaacag ggagcgagaa cggcagcgca gattgcgatg caactcgccg
     1321 agagcggtct gatcgagcaa ctgttccccg ggccggcgag ggcgaggcgg gcgctcgacc
     1381 gcctgatggc cgactcgtcg gcggtgaagc ggttcgatca agttgatctc gtgttcgtgg
     1441 acgaggcgca agacctcacg ctcgtcgagc tcttcctgct ggtgaggctg tgcgctgcga
     1501 tcggctcgtt ccgccaaggc gcaccaccgg ccttcgtagc cgccggcgac gagggccaga
     1561 cggtccgacc ctcggacttc gaatggggtc agctcgcgga tctcacggcg agccagatcg
     1621 gccgtcgcag cgaattcgag ctcacgggca atgttcgaag cccgcagaac atcgcgttgg
     1681 tcgtcaaccg ctcctgggac atgtaccgac acttcgcgaa ggatgagcgg ccgcgcgggt
     1741 acgcgagcgc ggagatcgag gaggcgtctg cgggacgagt gatctacacg acgtgccgga
     1801 cgccggagga tcttcgggag atcattgaag tcttcgacgg gattcccaac gccgcgctcg
     1861 tctatccggg ctaccgggtc cccgacagct atcggtccgt ggcttccgcg acctcggacc
     1921 tggtgctgac gagccggtcc gcgaaggggc tggacttcca gaccgtgggg gttctggacg
     1981 ccggccatca gatggcgcgg ttgaaggaac tcgcgctgga cgccggcccg gacagtcgcc
     2041 tctccgcgct ctggggtcgg atcctcgccg atcatctgcg agtcgcgctg agccgcgcaa
     2101 ccgagaacct ggtcctgatc gatgtagagc ccggcgacga agcgcgcgcg gaggtcgaga
     2161 cgctgtgtgc cgacgcccag ctcaccgaga tggaacccga ggagctggtc acattcctcc
     2221 gcagacagga cgagagcgac gccgcggagc tcgctctaga gttctgcgag gaagccgaga
     2281 agcttctcgg gaaccaatca gttcgcgcgt accggcgatc tcgacaggcc gtagtgctgc
     2341 tgggcgatgc caagtcgccc aacgcggtgc aggatgccgc actccggaga caagcatgga
     2401 cgttgcgcgg cgtcgcagcg gccgagctcc ttcgccatcg cgccggacgc cagaaggtcc
     2461 cgtactccgc cgatgagctc ctcaccgacg ccgtctcttc gcttcggcgg gcagagcggg
     2521 gggcggatgc tcaggcgatc cagctcatca accgcttcgt caccgggtcg gcaggaccac
     2581 acagcttggt cggcgaggtc aaggaacttt tgccgcttct cccgagcctc gagcgccaca
     2641 tcgagcgcta cgcccgggag gtgatcgtga gctggt
//
LOCUS       DATMBB010000200        15349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_139134, whole genome shotgun sequence.
ACCESSION   DATMBB010000200 DATMBB010000000
VERSION     DATMBB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139134"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VNL92_04325"
     CDS             complement(<1..987)
                     /locus_tag="VNL92_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWO93968.1"
                     /translation="MDQRLLDEVALSREEYDRIVELLDREPNEVELGMFGALWSEHCG
                     YKNSRPLLRRLPSEGPRVLTRVGAENAGAVDIGGGLCVVMKIESHNHPSAVEPYQGAA
                     TGVGGIVRDIFAMGARPIALLDSLRFGALDDAHQRYLFEGVVGGIGGYGNCLGIPTVG
                     GELQFDDAYAGNPLVNAMCVGIAKVGQLLSARASGPGNPIMLVGSETGRDGIHGASGL
                     ASRTEPAARFEELRPAVQVGNPFLEKLLMEACLELAEHRDWIVGLQDLGAAGLTSAAI
                     ECAHRGECGVDIDVALVPRRERGMTPYEVMLSESQERMLVIARKEHEEDVRRL"
     gene            complement(1012..1593)
                     /locus_tag="VNL92_04330"
     CDS             complement(1012..1593)
                     /locus_tag="VNL92_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012394487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HWO93969.1"
                     /translation="MGYPVEYEIEYPEHLSRWMWLVKWLLAIPHFIILDLFGSLAFAV
                     AIISGFSLLFTGRYPAGLFDLVAAYLRWQTRTAAYLFLRDEYPPFSGAEEPQYPVRVR
                     VERPEGLSRWMWLVKWLLAIPHLIVLVFYGILALVLVIIGLIALLFTGRFPKGLFDLI
                     VGFSRWALRVQAYALYLLTDQYPPFSGRPAAEV"
     gene            complement(1654..2364)
                     /gene="purQ"
                     /locus_tag="VNL92_04335"
     CDS             complement(1654..2364)
                     /gene="purQ"
                     /locus_tag="VNL92_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWO93970.1"
                     /translation="MRFGIVVFPGTWSDRDCYHAISDVLGQEAEYVWHRDRDLSRFDA
                     VILPGGFSYGDYLRCGAIARFSPIMDAVAEFAESGRLVLGICNGFQVLCEAGLLPGAL
                     RRNADLRFHCEWVNLRTERTDTAFTNACLPSQVLRIPISHGEGNYYADERALEELEAA
                     GRVVFRYCDAKGDARPDANPNGSVHNIAGIINGRGNVLGMMPHPERAVESLLGSDDGI
                     LILRSMVTAGVGMLTASR"
     gene            complement(2412..4607)
                     /locus_tag="VNL92_04340"
     CDS             complement(2412..4607)
                     /locus_tag="VNL92_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWO93971.1"
                     /translation="MRREQGGRSAAAIVAFTAALAVGVFAILFPIGSAGIDVQEGERA
                     PRTVQAPRDASYESEVLTQRLRDEAAASVQPTLVFDAGVADRQLRRLDEILAAISSIR
                     SANMDATQKSAALTNIDGLSLSPRAAVLAVAFTEEEWSVVEAEAREALAATYEGSVGP
                     TDLDAAQQALSARVQGQLGEGVALLVEELARPLVVPNQIVDEAATERAREEARARIAP
                     AMVDVVRGEVIIREGETVDSLALEKLERLELLRSDLPRSDIAGVAIFAALFAATLGLY
                     VAFGPSQALGAEHRLPLLGFILLATAAMARFGLDAFLPDEEDLSLSYVLPLAAAPMLT
                     VALVDIPAGLLVAALTAVLAGFAGYYHIDATEAAAVRPIDTVALIATVFLGGVAGAFA
                     THRAQRLNAYLLAGLAVAGVTAVTILVFWLLDPSRSLGDIPWFVGTALGGGVGAALLT
                     IGALALAGSALRVTTSLRLMELAQLEQPLLRRLREEAPGTFHHSVIVSGLAERAAEAI
                     GADALLVRVGCYYHDIGKTLQPRFYIENQGGEGNPHDELDPEESARIIIQHVLGGEDL
                     ARRQGLPDAVRAFIREHHGRRLVTYFYHRAAERDPEAQEGAFRYPGPRPQSRETAIVM
                     LADSTEAVVRSATDRSPERIDELVDSVIAERLTEGELDESDLTLRNLADIARAFKATL
                     RGVYHPRIEYPSATGTAQQRAAEASRGVQLPMPMDGVSFPTTRDAPIRR"
     gene            complement(4707..5156)
                     /locus_tag="VNL92_04345"
     CDS             complement(4707..5156)
                     /locus_tag="VNL92_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWO93972.1"
                     /translation="MSLEEKVAGDLREALRSRDQVRLSALRMLAAALHNARIGAGRDL
                     ADGEALAVVQREVKQRRDSIEQFRAGGRQDLVDKETAEIAVLEAYLPRQLGRDEIAQE
                     ARSIIANVGAAGPSDKGKVMGPLMERLRGRADGRLVNEVVTELLAGH"
     gene            complement(5218..5421)
                     /gene="rpsU"
                     /locus_tag="VNL92_04350"
     CDS             complement(5218..5421)
                     /gene="rpsU"
                     /locus_tag="VNL92_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWO93973.1"
                     /translation="MADIIISETESFESALKRFNKRVQQDGVLAESRRREHYEKPSVK
                     RRKKEAARIRKIMKAKKKAAARM"
     gene            complement(5458..6993)
                     /locus_tag="VNL92_04355"
     CDS             complement(5458..6993)
                     /locus_tag="VNL92_04355"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWO93974.1"
                     /translation="MRVLLAHAAVHTPLEQARSVLIDGERVLALDASESEAEAVIDLR
                     GRAVVPAFVDAHGHLLAFAASLMSVDCRGAKDMADLQSRIRAVAERTPAGEWIRAYGY
                     DENDLTEGRHPTRSDLDGAAPDHPVRLAHRGGHAVVLNTLALRRCGVDTEIAEPAGAY
                     VDREVPSGEPSGLFFEMGDVLDARIPPISPAEMDLALAQAEVALLRAGVAAFHDCGAR
                     NGPGEWESLSRLSSERDFRPRLVAASGWPAWSEGYDIRELAPAVAGSHVKLMLSELGD
                     DVAPDADELSERVARVHAAGHDVAIHAVTARGVRAAVDAIDRALALHPRRDHRHRIEH
                     ASICSPELMPRMAALGITVVSQPAFIYANGERYLRTVPPADLPNLYAFGSMAEAGVHL
                     AFGSDCPFGRVEPLIGLRSAYERRAANGAVLCGRPMWPAAALAASTLGAARAAGIEDE
                     HGWIRPGARADLVGLSKAPESREAQIEAVLISGRVVAGRASLVERLETFLPIDTQKAG
                     VLW"
     gene            complement(7007..8950)
                     /gene="mnmG"
                     /locus_tag="VNL92_04360"
     CDS             complement(7007..8950)
                     /gene="mnmG"
                     /locus_tag="VNL92_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HWO93975.1"
                     /translation="MEVVPYEVVVIGAGHAGCEAALASARMGLRTLLLTMNLDTIALM
                     PCNPSIGGPAKGHLVREIDALGGEMGRIADQTFVQIRLLNSAKGPAVQALRAQSDKRL
                     YGLAMKRTLERTPNLQLRQAMVESIERTGEDAGRPTLDVMTNTGRTYRAQALVVTTGT
                     SLAGRIIIGDVSHEGGRTGEASARGLSASLRRLGFELGRLKTGTPPRLDARTLDYDAA
                     VPQPGSDMPLSFSFDGPPGDPYLLTPNPVFPAHPPFAWRPQMHCFLVHTIPATHEIIR
                     ANLHRAPMFNGTIESRGPRYCPSIEDKVVRFADKTSHGLFLEPEGWETNEVYLQGANT
                     SLPEDVQWAMVRSVPGLESAEIMRLGYAIEYDYVKTHQTSVSLESRLVPGLFFAGQIN
                     GTTGYEEAAGQGIVAGINAARYVRGQAPLILRRDQAYLGVMLDDLVTRELTEPYRLFT
                     SRAEYRLLLRQDNADLRLTPIGRELGLVNAERAQRVEAKREAIEGELGRIRRTYLKPS
                     DELRERAIELGLGDIVRSLSEEELLRRPEATYAHLLALGLGDPRLAADVAEQVAIEVK
                     YAGYIERQGREVARVRRLEEQRVPADIDYAAIPGFRREAAERLAHFRPATVGQAGRLP
                     GVTPADIAVLLVQLERQAKVPAY"
     gene            9041..10678
                     /gene="murJ"
                     /locus_tag="VNL92_04365"
     CDS             9041..10678
                     /gene="murJ"
                     /locus_tag="VNL92_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWO93976.1"
                     /translation="MRRVALPLRRGRLSALFGGQQSIAAAAILVAAGYLLSRVLGVVR
                     TAAIARAFGTESDLAAYWVAFRLPDLIFQLVAGATLASAFIPAFTNVATNEGERAAWR
                     LASSVINLVLLATIAVAILGAILAPVVVPLLAPGLGEDTGREAELQDLAVELTRWMLI
                     SSVLFSVSGMIMGVLNARRHFLAPALAPAVYNLSIIVAAVALTGPFGVHGLVVGVIAG
                     AALHLAVQLPALVRAGMRYSPIAEWTDRRVREVFRLMVPRTIGLAAAQANFFITTIFF
                     ASFIGDEAISALTYAWLLMTLPLALFGMAIGTAVFPEMAESAARADAERLREMVARAL
                     RLILYLTIPASVGLILLGRPIIALLLQRGEFDAADAELTAAALTFFSVGLFAHGAIEI
                     LARGFYAISNTSTPMRMALLSMAVNAVLSLAFVGPFEVDGLAVALSVATTIEAALLYV
                     TLRREFGDLDEREAIVSLRRTLVGTALLAEVVLFFVLGFNLDDPGVGAFLGVVCAIGL
                     GAVSFFLATWLLRSEEADLLLRRGLAFLRSRRGAEPR"
     gene            complement(10675..11313)
                     /locus_tag="VNL92_04370"
     CDS             complement(10675..11313)
                     /locus_tag="VNL92_04370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWO93977.1"
                     /translation="MTRLLLVRHGESQGNAEHRLQGTVPYPLTELGRKQGEALAERVA
                     AEGADVLYTSPTLRARETGEIIAQRIGLRLDVLEGVREYNFGVLSGLTMAEIAQRHPA
                     WVHALREGGPVPLAEGEEGHESFRERVVSSLWGLVDKHPEDVVAVVTHSGVISVFCQD
                     VSGARSMQAINVFGTENCSLTVVDALPPSALPPGMPRGVIVAFNDVCHTRAS"
     gene            complement(11310..11999)
                     /locus_tag="VNL92_04375"
     CDS             complement(11310..11999)
                     /locus_tag="VNL92_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93978.1"
                     /translation="MLANLSVGARVACFVLLVVAGALTALLLLGSADAQTAGQAIVTF
                     ETADGSTYRVLLTDPDDIARAEEALAGDGRAGIPNGELRPGDGGVNTGHAWHVVDVHF
                     ADLTIELCDGTASMVDENLDYWLNVVGSFCPWSARVVDVSTADAGCTAVVDAPPTGSL
                     GSVVLQGCRAPQEATEAIEAATNRPVAALWQLGGGRWLYYLPAHPSAGSLDQVSDPAA
                     AIVVFGSEGLG"
     gene            complement(12004..12609)
                     /locus_tag="VNL92_04380"
     CDS             complement(12004..12609)
                     /locus_tag="VNL92_04380"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWO93979.1"
                     /translation="MQITRDALGRYTPRQLDLAPGQMSSAVLVPLYVESSRYHILFTK
                     RSDHVEHHKGQISFPGGAQDPDDPDLLHTALRETHEEIGVDPADVEVLGQLDDFVTIT
                     NFRVRPYVGLIRRSPYEFIYNRHEVAEVLVVPVSHLVDDRNIAEELRQVGERQVMMRS
                     YVFEDHVIFGATAFMLRAFLDLLAEEGSIEELIREIAEEVR"
     gene            12916..13404
                     /locus_tag="VNL92_04385"
     CDS             12916..13404
                     /locus_tag="VNL92_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93980.1"
                     /translation="MRRLLTFVLLLTIVGSIALACGDDDDGEDTPGAAGPPSKEGVLP
                     TVAAPEVTIPPLDATPAPNVQVIPNGGEWTGGQNGVVVRGTGSAEGLAAAAREASGRE
                     VLAIWFLSGGVYRWSVPSLPQLQDGFVQTESDPLSTIFVLELEEGEEPVEVPTIEATA
                     QE"
     gene            complement(13434..14546)
                     /locus_tag="VNL92_04390"
     CDS             complement(13434..14546)
                     /locus_tag="VNL92_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWO93981.1"
                     /translation="MAAGRRRGVGLAGRMAVAGLAAIGAQRLYGLAVQRAVRRRALRL
                     EEGVPYDAHTGFCLDRGEPLYLVRHEHDATLFFFNGFRLRSAPGMYAEWLQSMHERLG
                     LNVIAPALGVQSAPYDVRSRGWCFLQDIRTAIQVYDAYAAQAGPGHRLILFSHSYGSI
                     VTLTLAAKRSPSHAILLSPVPHRMGTPMVVRRMQGPVGPLLSRAMDRTLDEEGGLAPG
                     RLSWLPHVLPLYVRRGSSGGWDVADQELRRRANAEIGNVPEVGTADLLELLRAILFAR
                     ERVLPHLSNRTFTLIYEGRDLVYPPELQHELAERLRSNGNVVDEHHLPDSAHDILLDR
                     DAALAKDIIEGAIANVMGGSETSEGMTPSWLRADGG"
     gene            complement(14519..15334)
                     /locus_tag="VNL92_04395"
     CDS             complement(14519..15334)
                     /locus_tag="VNL92_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWO93982.1"
                     /translation="MPHATVNGIQLYYEEEGAGFPVVFIPGFTETCAMWSFQRAPFAQ
                     RYRFIAYDLRGHGRSSAPDDSAAYSPDIFVEDTKGLLDALGIERAHVVGHSLGGAVAL
                     RFALAHPDRIDCLVVVNSNSGAAPDEWREQVEPRMHGMAETLRRKGVMALADGMINRR
                     LPPAELEARRREFAALSPAGIAHTATQVLPYISSLEKAERISCPTLLVVGERDRDFVV
                     RSDWLMERMPNCRKVVLAGAGHAANEHAPEAFNEAVLAFLAEREPWRPGDAGA"
BASE COUNT         2566 a         5194 c         5099 g         2490 t
ORIGIN      
        1 caggcgccgc acatcttcct cgtgctcctt gcgggcaatc acgagcatgc gctcctgcga
       61 ttcggagagc atgacctcgt acggagtcat gccccgttcc cgccggggca cgagggccac
      121 atcgatgtcg acaccgcact cgccccgatg ggcgcactcg atggcggccg aggtaaggcc
      181 ggccgcgccg aggtcctgga ggcccacgat ccaatcgcgg tgctccgcga gctcgaggca
      241 cgcctccatg agcagcttct cgaggaatgg gttccccacc tggaccgcgg gacgcaactc
      301 ctcgaagcgg gccgcgggct cggttcgcga tgccaggccg gaggcgccgt ggatgccgtc
      361 gcgacccgtc tcggagccca cgagcatgat cggattcccc gggccgctcg ctcgcgccga
      421 cagaagctgg cctaccttgg caatgccgac gcacatggcg ttgaccagcg gattaccggc
      481 atatgcgtcg tcgaactgca gctcgccgcc gacggtcgga ataccgaggc agttcccgta
      541 cccgccgatg ccgcccacca cgccctcgaa cagatagcgc tgatgcgcat cgtccagcgc
      601 gccgaagcgg agcgagtcga gcagagcgat ggggcgggcg cccatggcga agatgtcccg
      661 gacgatgccg cccacacccg tggccgcccc ctggtacggc tccacggccg acgggtggtt
      721 gtgcgattcg atcttcatga cgacgcagag gccgccgcca atgtcgaccg cgccggcgtt
      781 ctccgcgcca actcgtgtca gcacccttgg gccctccgaa gggagacggc gcagcagcgg
      841 acggctgttc ttgtagccgc agtgctcgct ccagagggca ccgaacatgc cgagctccac
      901 ctcgttcggc tccctatcga ggagttcgac gatgcgatcg tattcctctc gcgagagcgc
      961 gacttcgtcg agcaggcgct ggtcaatggc caagtggcgg acctccggta gctagacttc
     1021 tgcggccggc cgtccgctga acggcggata ctggtcggtc agcagataca atgcatacgc
     1081 ctggactcgc agcgcccagc ggctgaaacc gacgatgagg tcgaacaggc ccttcgggaa
     1141 gcgtccggtg aagagcagcg cgatgaggcc gatgatcacg agcactaacg ccaggatacc
     1201 gtagaagacc agcacgatga ggtgcggaat ggcgagcagc catttgacga gccacatcca
     1261 ccgagagagt ccttccggcc gctcaacacg cacgcgaaca gggtattgcg gctcctcggc
     1321 gccgctgaag ggcggatact cgtcccgcag aaacaggtag gcggccgtcc gcgtctgcca
     1381 gcggaggtag gctgccacga ggtcgaatag gcctgccgga tagcgaccgg tgaataacag
     1441 ggagaatccg gagatgatcg caaccgcgaa agccaaggag ccgaacaggt cgaggatgat
     1501 gaaatggggg atggcgagca gccacttcac cagccacatc cagcgagaca gatgctccgg
     1561 atactcgatt tcgtactcga ccggatagcc cattttgcgt cccctttcgc tgagcgttcc
     1621 ggcccagccg ggacccgaag gacgaggggg ttcttatcgg ctggcggtca gcatgccgac
     1681 tccggcagta accatggagc ggaggatgag gatgccgtca tcgctgccca gcagcgactc
     1741 gacggcgcgc tcggggtggg gcatcatgcc gagcacgttg ccacgaccat tgatgatgcc
     1801 ggcgatgttg tggacggagc cgttggggtt cgcgtcgggg cgggcgtcgc cctttgcgtc
     1861 gcagtagcga aacactacgc gcccggccgc ctcgagctcc tcgagggccc tctcgtcggc
     1921 gtagtaattg ccctcgccat gggagatggg aatgcgcagg acctgcgagg gcaagcaggc
     1981 gttcgtgaag gcggtgtccg tacgctcggt gcgcaagttc acccattcgc agtgaaagcg
     2041 caggtcggcg ttgcggcgca gcgctcccgg caggaggccc gcttcgcaca gcacctggaa
     2101 tccgttgcag atgcccagaa cgagacggcc gctctccgcg aactcggcga ccgcatccat
     2161 gatcggcgag aagcgagcga tcgcaccgca tcggagatag tcgccgtagg aaaagccgcc
     2221 tggaaggatg acagcgtcga agcgcgacag atctcggtcc cgatgccaaa cgtactcggc
     2281 ctcctggcca agcacgtccg atatcgcgtg gtagcagtcg cgatcgctcc atgtgccggg
     2341 gaagacgacg atgccgaagc gcatcaatcc ctccgctcgt agtatacccg gcccgggacc
     2401 acgcttcccg gctagcgacg tatgggggcg tcgcgggtcg tgggaaacga gacgccatcc
     2461 atcggcatcg ggagctggac gccccgggaa gcctcggcgg cgcgctgttg ggccgtcccc
     2521 gttgcagacg ggtattcgat acgcgggtgg tacacgcccc ttagtgtggc cttgaaggcg
     2581 cgagcgatgt cggccaaatt ccgcaacgtc aggtccgatt cgtccagctc tccctcggta
     2641 agacgctcgg cgatgacgct gtcgaccagc tcatcgatgc gctccggcga acgatcggtg
     2701 gccgaacgca cgacggcttc cgtcgagtcg gcgagcatga cgatcgcagt ctcgcggctt
     2761 tggggccgcg gcccggggta acggaacgct ccttcttggg cctccgggtc tcgttcggct
     2821 gcccggtggt agaagtaggt gacgagccgc ctgccgtgat gctctcggat gaaggcgcgg
     2881 acggcgtcgg gtaggccctg ccgccgagcc aggtcctcgc cgccgagcac gtgctgaatg
     2941 atgatgcgtg cgctctcctc cggatcgagc tcatcgtgcg ggttgccctc gccaccctgg
     3001 ttctctatgt agaagcgggg ctgcagcgtc ttgccgatgt catggtagta acacccgacg
     3061 cgcacgagga gggcgtcggc gccgatggcc tcggcggcac gttcggccaa cccgctcacg
     3121 atgacgctgt gatggaatgt gcccggcgcc tcttcccgta aacgccgcaa cagcggttgc
     3181 tccagctggg caagctccat gaggcgaagg ctggtcgtga cgcgaagcgc cgagccggcg
     3241 agggcgagag cgccaatggt caacaaagcc gctccaactc cgccaccgag cgccgtaccc
     3301 acgaaccacg gaatgtcgcc cagcgaccgc gaaggatcga ggagccagaa caccaggatg
     3361 gtcaccgcag tgaccccggc gaccgcgagc cctgcgagca gatatgcgtt gagccgctgc
     3421 gcccgatggg tcgcgaacgc gccggcgacg ccacccagga acaccgtcgc gatcagggcg
     3481 acggtatcga tgggacggac cgccgcagcc tccgtggcat cgatatggta atagcccgcg
     3541 aatccggcga gcacagccgt cagcgcggcc acgagcaggc cggcgggaat gtcgaccagc
     3601 gcgacggtca acatcggtgc cgcggctagc gggaggacgt agctcagcga aaggtcttcc
     3661 tcgtcaggga ggaacgcgtc gaggccgaac cgggccatcg cggccgttgc gagcagaatg
     3721 aagccaagca gcgggaggcg atgctccgca cccaatgcct gcgatgggcc gaacgcgacg
     3781 tagaggccaa gggtggcggc gaacagggcc gcgaagatgg ccacgccggc gatgtcggag
     3841 cgcggcaagt cggaacggag cagctccaac ctttcgagct tctcgagtgc cagcgaatcg
     3901 acggtctcgc cttcgcggat gatgacctcg ccccggacga catctaccat cgcgggcgcg
     3961 attcgagcgc gcgcctcttc gcgcgctcgc tcggtcgcgg cctcgtcgac gatctggttc
     4021 ggtacgacga gcggcctcgc cagctcctcg accagcaaag caacgccctc gccgagttga
     4081 ccctgaacac gggcagagag ggcctgttgc gccgcgtcga gatcggtggg gccgacggat
     4141 ccctcgtagg tggccgcgag cgcctcgcga gcttcggcct caacgaccga ccattcctcc
     4201 tcggtgaagg ccacggcgag gacagcagcg cgcggcgaca gtgagaggcc atcgatgttg
     4261 gtaagcgcgg cgctcttctg cgtcgcgtcc atgttcgcgg aacggatgct ggagatcgcc
     4321 gcaaggatct cgtcaaggcg ccgcagctgc cggtctgcca cgccggcgtc gaacacgagc
     4381 gtcggttgca ccgacgccgc ggcctcgtcg cggaggcgct gggtcaggac ctcgctctcg
     4441 tacgaggcat cgcgcggtgc ttgcaccgtc cgcggagcgc gctccccctc ctgcacgtcg
     4501 atgccagcgg agccaatcgg gaacaggatg gcgaacacgc caacggcgag ggccgccgtg
     4561 aaggcgacga tggccgccgc gctgcgaccg ccctgctcgc gtcgcatgct ccctccgtgc
     4621 aatccggatg gcgccataca tggacgaggc gcgccagttg cgacctcatc gtaacagtcc
     4681 gtgggattgg gagaaacgaa tcggggtcaa tgtccggcga gcagctcggt cacaacctcg
     4741 ttgacgagac gcccatcggc gcggccacga aggcgctcca tcagcggtcc catcaccttg
     4801 cccttatccg atggaccagc agcgccgacg ttcgcgatga tggaccgtgc ctcctgcgcg
     4861 atttcgtcgc gtcccagctg ccgcggcaag tacgcctcga gcacggcgat ctccgccgtc
     4921 tccttatcga ccaggtcctg ccgtccgccg gcgcggaact gctcgatcga gtctcgccgc
     4981 tgcttgacct ccctctggac gacggcgagg gcctcgccat ccgcgaggtc gcggcctgct
     5041 ccaatgcggg cgttatggag cgcggccgca agcatgcgga gggcgctgag acggacctgg
     5101 tcacgcgagc gcaatgcctc gcgcaggtcg cccgcgacct tttcctcgag gctcatacgg
     5161 tctgatctct ccgggtgaac gagatgacgg cccgatggcc accggaccgg ctgcgggcta
     5221 catccgagcg gcggccttct tcttcgcctt catgatcttg cggatccggg ccgcttcctt
     5281 cttgcgccgc ttcacactcg gcttctcgta gtgctctcgc cgtcgcgact cagcgagaac
     5341 cccgtcctgc tgtacccgct tattgaaacg cttgagcgca gattcgaagc tttcggtttc
     5401 gctaatgatg atatctgcca gaaacctccc ctcccttcct atcgagactg ccgaacgtca
     5461 ccatagtacg cccgccttct gggtgtcaat tggcaggaac gtctccaggc gctccaccag
     5521 gctcgccctc cctgcgacga cacgaccgct gatcagcacc gcctcgatct gcgcttcccg
     5581 tgactcaggc gccttgctca gcccaaccag atcggcacgc gcgcccggtc ggatccagcc
     5641 gtgctcgtcc tcgatgccgg ccgcacgcgc ggcgcccagc gtcgaagcgg ccagcgcggc
     5701 cgccggccac atgggccgcc cacagagcac cgctccgttg gcggcgcgcc gttcgtacgc
     5761 cgaacgcagg ccgatgagcg gctcgacccg cccgaacggg caatcggagc cgaacgcgag
     5821 gtgcacgccg gcctctgcca tcgatccgaa cgcgtagagg ttcggcaggt ccgccggcgg
     5881 gacggtccga aggtagcgct cgccgttcgc gtagatgaag gcaggctggc tcaccacggt
     5941 gatgcccaac gccgccatgc gcggcatgag ctcgggggaa cagatggagg catgctcgat
     6001 tcggtggcga tggtcgcggc gcggatgcag cgccagggcg cgatcgatcg cgtcgaccgc
     6061 ggcacgcacc ccgcgggcag tgacggcatg aatcgccacg tcgtgtcccg ccgcgtgcac
     6121 gcgcgccacg cgctcgctca actcgtcggc atccggtgcc acgtcgtcgc ccaactcgct
     6181 cagcatgagc ttgacgtgcg agcccgccac tgccggcgcc agctcgcgaa tgtcgtaccc
     6241 ctccgaccac gcgggccagc ccgatgccgc gacgagccgc gggcgaaagt cacgctccga
     6301 ggacagccgg gaaaggctct cccactcgcc aggaccgttg cgcgctccgc agtcgtggaa
     6361 cgcggccacg cccgcacgga gcaaagcgac ctcggcctgg gccaaggcga ggtccatctc
     6421 ggctggcgag atcggtggga tgcgcgcgtc caggacgtcg cccatctcga agaagagacc
     6481 gctgggctct ccgctcggca cttcgcggtc gacgtaggcg cccgcgggct ctgctatctc
     6541 tgtgtcgacg ccgcatcggc gcaatgcgag cgtgttgagc acgacggcat gtccacctcg
     6601 atgagcgagt cgcacgggat gatctggcgc tgccccatcg aggtcgctcc tcgtgggatg
     6661 tcggccctcc gtgaggtcgt tctcgtcgta gccgtaggcg cgtatccact ctcccgccgg
     6721 cgtccgctcg gctaccgccc ggatccgcga ctggagatcg gccatgtcct tcgcgccgcg
     6781 gcaatccacc gacatgagcg acgccgcgaa ggccagcaag tggccatgcg catccacaaa
     6841 tgcgggtacg acggctcgcc ctcgcaagtc gatgacggct tcggcctccg attccgaagc
     6901 gtcaagggcg agaacgcgct cgccatcgat cagaaccgag cgagcttgct caagcggcgt
     6961 gtggaccgcc gcgtgcgcca agaggacacg cacgttggct ctcccctcag tacgcgggca
     7021 ccttcgcctg tcgctcgagc tgaacgagga gcacggctat gtcagctgga gtgacgccgg
     7081 gcagccggcc cgcctgtccg acggtggccg ggcgaaaatg agccagtcgc tccgccgcct
     7141 cccgccgaaa ccccgggatg gccgcatagt cgatatcggc agggacgcgc tgttcctcga
     7201 ggcggcgcac gcgcgccact tcccggccct gccgctcgat gtagccggcg tacttcactt
     7261 cgatggcgac ttgctcagcg acatccgcag caagccgcgg atcgccgaga ccgagcgcga
     7321 gaagatgagc gtacgtcgct tccgggcgtc gcagtagctc ttcctcgctc aggctccgga
     7381 cgatgtcgcc caggcccagc tcgatcgcgc gctcccgcag ctcatcgctc ggcttcaggt
     7441 acgtccggcg gatgcggccc agctcgccct cgatcgcctc gcgcttcgcc tcgactcgct
     7501 gagcccgctc cgcgttgacg agaccgagct cgcggccgat gggcgtgagc cgcaggtcgg
     7561 cgttatcctg ccgcaacagg agccggtact cggcgcgcga cgtgaagagc cggtagggct
     7621 cggtcagctc cctcgtcacg aggtcgtcga gcatgacgcc gaggtaggcc tgatcgcggc
     7681 gcaggatgag cggcgcctgc ccgcgcacgt aacgagccgc gttgatgccg gcgacgatgc
     7741 cctgacccgc ggcctcctcg tagcccgtcg tcccattgat ttgcccggcg aaaaacagcc
     7801 ccggcacgag gcgcgactcc agcgagacgc tcgtctggtg ggttttcaca tagtcgtact
     7861 cgatggcgta gccaagccgc atgatctcgg cgctctccag gcccggcacc gatcggacca
     7921 tggcccactg cacatcctcc ggaaggctcg tattcgctcc ctggaggtac acctcgttcg
     7981 tctcccagcc ttcgggctcg aggaagagac catgcgatgt cttgtccgcg aatcggacga
     8041 ccttgtcctc gatcgaagga cagtatcgcg gcccgcgcga ctcgatagtc ccgttgaaca
     8101 tgggcgcccg gtgaaggttg gcgcggatga tctcgtgggt cgcgggtatc gtatgtacga
     8161 ggaaacagtg catctgcggg cgccacgcga acgggggatg cgccgggaac acggggttcg
     8221 gcgtcaagag atacgggtcg cccggcggac catcgaacga gaacgagagc ggcatgtcgc
     8281 ttccgggttg gggaaccgcg gcatcataat cgagcgtgcg agcgtcgagg cggggcggcg
     8341 tcccggtctt gagccggccg agctcgaacc ccagacgccg gagcgaggcc gagagacccc
     8401 gggctgatgc ctcgcccgtg cgtccgccct cgtggctcac atcgccgatg atgattcgac
     8461 cagcgagcga cgtgccggtc gtgacgacga gcgcctgcgc ccgatatgtc cgtcccgtat
     8521 tcgtcatgac gtccagcgtg ggccgcccgg catcctcgcc cgttcgctcg atgctctcca
     8581 ccatcgcttg gcgaagctga aggttcggcg tgcgttcgag ggtccgcttc atggccagcc
     8641 cgtagagccg cttgtccgac tgcgcgcgga gggcctgcac ggccggcccc ttcgcgctgt
     8701 tgaggagccg aatctgcacg aaggtctgat cggcgatacg gcccatctcg ccgccgaggg
     8761 catcgatttc ccgcacgagg tgtcccttcg ccgggccgcc tatcgacgga ttgcagggca
     8821 tgagggcaat cgtgtcgagg ttcatcgtca ggagcagggt gcggaggccc atgcgagccg
     8881 aagcgagggc cgcctcgcag ccggcgtgcc ctgctccgat caccacgacc tcgtacggca
     8941 cgacttccat ggtgcgatcc tagcgtctcc ggcactgcga aggaagggcg ccggttcgtc
     9001 gctgagcgag gcggccgcta tactcgcgcg aggaggcggc atgcgccgag tcgcgcttcc
     9061 gctgcgcagg gggcgcctga gcgccctgtt cggtgggcag caaagcatcg cggccgcggc
     9121 catcctggtg gccgccggct atctcctgag ccgggtgctc ggtgttgtac gcacggcggc
     9181 catcgcgcga gccttcggca cggagtcgga cctcgccgcg tattgggtcg cctttcgcct
     9241 acccgacctc atctttcagc tggtagccgg cgcaaccttg gccagcgcat tcatccccgc
     9301 cttcacgaac gtggcgacga acgaaggcga acgggcagcg tggagactcg ccagctccgt
     9361 catcaatctc gtgctgctgg cgacgattgc cgtcgcgata ctcggcgcga tccttgcgcc
     9421 cgtcgtcgtt cctctgctcg cgcctgggct tggcgaggat acgggccgcg aggcggaact
     9481 ccaggaccta gcggtcgagc tgacccgctg gatgctgatc tcgtcggtcc tgttctcggt
     9541 cagcggcatg atcatggggg tgctgaacgc gcggcggcac ttcctggcgc cggccctcgc
     9601 gcccgccgtg tacaacctgt cgatcatcgt ggctgcagtc gccctcacgg gcccgttcgg
     9661 cgtacacggg cttgtcgtcg gggtcatcgc gggcgccgcg ctgcacctgg cggtacagct
     9721 gcccgccctg gtgcgtgccg gcatgcgcta ttcgcccatc gccgagtgga ccgaccgccg
     9781 ggtccgcgaa gtgtttcgac tcatggtgcc acggaccatc ggacttgcgg cggctcaggc
     9841 gaacttcttc attacgacca tcttcttcgc gtcattcatc ggcgacgagg ccatctccgc
     9901 cctcacgtac gcttggctgc tgatgacgtt gccgctcgcg ctgttcggca tggcgatcgg
     9961 cacggccgtc ttcccggaga tggccgagag cgcggcccgt gcggacgccg agcggctgcg
    10021 ggagatggta gcgcgtgccc tccgtctcat cctctacctc accatcccgg cgagcgtcgg
    10081 cctcatcctc ctcggccggc cgatcatcgc cctgctcctc caacgaggtg agttcgatgc
    10141 cgcggacgcg gagctcacgg ccgccgcgct cacgttcttc tccgtgggct tgtttgcgca
    10201 cggcgccatc gaaatcctgg cccgcggatt ctacgccatc tccaatacga gcacgccgat
    10261 gcgcatggcc ctgctctcca tggccgtcaa cgccgtcctc agtctggcgt tcgtcggtcc
    10321 cttcgaggtg gatggccttg ccgtggcgct gtcggtcgcc acgaccatcg aggcggcctt
    10381 gctgtacgtg acgctgcgtc gcgaatttgg cgaccttgac gaacgcgagg ccatcgtttc
    10441 gctgcgccga acgcttgtcg ggacggcgct cctggccgaa gtcgtgctct tcttcgtgct
    10501 cgggttcaac cttgatgatc ccggtgttgg cgcgttcctg ggcgtcgtct gcgcgattgg
    10561 gcttggggca gtcagcttct tcctggcgac gtggctgctg cggtcggagg aagccgatct
    10621 tcttctgcgc cggggactgg ccttccttcg atcgcgcaga ggcgccgagc cgcgctagct
    10681 cgcgcgtgtg tgacagacgt cgttgaacgc gacgatgacg ccgcggggca tcccgggcgg
    10741 gagggcgctt ggcggcagcg catcgacgac ggtgagggaa cagttctcgg tgccgaacac
    10801 gttgatagcc tgcatcgaac gagcgccgga cacgtcctgg cagaagacgg agatgacccc
    10861 cgagtgggtg acgaccgcga cgacatcctc cggatgctta tccaccagcc cccaaaggga
    10921 ggagacaacc ctctcccgga acgactcgtg gccctcctcg ccctcggcca gggggacggg
    10981 gccgccctcg cggagcgcgt ggacccaggc aggatggcgc tgcgcgatct ccgccatcgt
    11041 cagaccggac agcacgccaa agttgtactc gcggacgcct tcgaggacat cgagccgcag
    11101 cccgatgcgt tgggcgataa tctcaccggt ctcgcgcgcc cgcagcgtcg gactcgtgta
    11161 tagcacgtcc gcaccctcgg ccgccactcg ctcggccagc gcctcgccct gcttgcggcc
    11221 gagctctgtc agcggatacg gaaccgtgcc ctgcagccga tgctccgcgt tgccctgaga
    11281 ttcgccgtga cggaccagga ggagtcgcgt catcccaatc cctcggagcc gaagaccacg
    11341 atggcggccg ccggatcgct gacctggtcg agcgagccgg ccgagggatg ggcggggaga
    11401 tagtagagcc aacggccgcc tcccagctgc caaagcgccg cgacaggccg gttcgtcgcc
    11461 gcctcgatgg cctccgttgc ttcctgcggc gctcggcagc cctgcagaac aacgctgccg
    11521 agcgaacccg tgggcggcgc atcgaccaca gccgtgcaac ccgcatccgc ggtcgagacg
    11581 tcgacgacac gcgctgacca ggggcaaaac gacccgacga cgttgagcca gtagtcgagg
    11641 ttctcgtcca ccatggaggc cgtcccgtcg cagagctcga tggtcaagtc ggcgaagtgt
    11701 acatcgacga catgccaggc atggcccgtg ttcacgcccc cgtcgccggg ccggagctcg
    11761 ccgttcggaa tgcccgcgcg tccgtccccc gcgagcgcct cctcggcacg agcgatgtcg
    11821 tccggatcgg tgagcagcac ccggtacgtg ctgccatccg ccgtttcgaa ggtgacgatg
    11881 gcctgcccgg cggtctgcgc atcggcggag ccaagcaaca gcaacgcggt cagcgctccc
    11941 gcgacgacga ggagaacgaa gcatgccacc ctggcgccca cactcagatt ggcgagcacg
    12001 aacctaccgc acctcttccg ctatctcgcg aatcagctcc tcgatggatc cttcctccgc
    12061 caggaggtcg aggaacgcgc gcaacatgaa ggcggtcgcc ccgaagatga cgtggtcctc
    12121 gaacacgtac gagcgcatca tgacctggcg ctcgccgacc tggcgcagct cctccgcgat
    12181 gttgcggtcg tcgacgaggt ggctgaccgg cacgacgagc acctcggcca cctcgtggcg
    12241 attgtagatg aactcgtacg gcgagcgccg gatgaggccg acatatgggc gcacacgaaa
    12301 gttggtaatc gtcacgaagt cgtcgagctg acccagcacc tcgacgtcgg cagggtcgac
    12361 gccaatctcc tcgtgtgtct cacgcagcgc ggtgtggagc aggtcagggt cgtcgggatc
    12421 ctgagcgccg ccgggaaacg agatctgacc cttgtggtgc tcgacgtggt cgctgcgctt
    12481 ggtgaagagg atgtgatagc ggcttgattc gacgtacaag ggcacgagga ccgcggacga
    12541 catctgtccc ggagcgaggt cgagttgacg gggtgtataa cggccaagag cgtctcgcgt
    12601 gatctgcacg gcgtcattgt cagcgtctcc ggcggcgcgg gtcaaccgat gcgcgtccac
    12661 cccgtcgttg ccggacgcat cgcttgtccc tgaactttcg tggcgctagg gtggaaccat
    12721 ggccattccc atccagcgtt gtccccgctg cggcgaacgc atgcccgata tcaagggctc
    12781 ccgtctctcg atctgccgcc gctgcggcta caaggacgat tgttgctgag gcccgagacg
    12841 gcgccggctt agctcgtcgc cggcatggac gccgtcccct gcatgtcaag gaaccacacc
    12901 ccgaggaggc aacgggtgcg tagactcttg acgtttgtcc tgctcctgac catcgtgggc
    12961 tcgatcgctc tcgcctgcgg cgatgacgac gacggcgaag acactccggg cgccgctggt
    13021 ccgcccagca aggagggtgt gcttcccacc gtagccgccc ctgaggtgac gataccgccc
    13081 ctcgatgcga cgcctgctcc gaacgtgcag gtcatcccga acggcggcga gtggacgggc
    13141 ggccaaaacg gtgtcgtcgt gcggggcacg ggatccgcgg aagggcttgc cgcagcggcg
    13201 agggaggcaa gcggccgcga ggtcctcgcg atatggtttc tctcgggtgg cgtataccgg
    13261 tggtcggtgc cgagcctgcc ccagcttcag gacggattcg tgcagaccga aagcgatccg
    13321 ctcagcacca tcttcgtgct cgagctggag gaaggcgagg agcccgtcga ggtgccgacc
    13381 atcgaggcga cggcgcaaga atagcgcgaa tcggactact ccggtacacg gagtcatcct
    13441 ccatcggctc gcagccacga gggcgtcatg ccctcggacg tctccgatcc ccccatcacg
    13501 ttcgcgatgg caccctcgat gatgtctttc gccaaggcgg catcgcggtc cagcaggatg
    13561 tcgtgagcgg agtccggcaa gtgatgctcg tcgaccacgt tgccgttgga acgtagccgc
    13621 tccgccagct catgttggag ctctggcggg tacacgaggt cgcgcccctc gtaaatgagc
    13681 gtgaacgtgc ggttgctcaa atgaggcaga acgcgctcgc gcgcaaacag aatggcccgc
    13741 aagagttcga ggagatcggc tgtccccacc tcgggcacgt tgccaatctc ggcgttcgcc
    13801 ctccggcgca actcttggtc ggcgacgtcc catcccccgc tgctccctct acgaacgtag
    13861 agagggagga cgtgcggcag ccacgagagg cgccccgggg ccaggcctcc ctcctcgtcg
    13921 agcgtccggt ccatggcgcg gctgagcagc ggccccaccg gcccctgcat gcggcgcacg
    13981 accatcggtg tgcccatgcg gtgagggacg ggcgagagca gaatcgcatg cgacgggctg
    14041 cgcttagccg cgagcgtgag cgtgacgatc gacccgtacg aatgggagaa caggatcagc
    14101 ctgtggcctg gacccgcctg ggctgcatag gcgtcgtaga cctgtattgc ggtcctgatg
    14161 tcctgcagga agcaccatcc gcgcgaccgt acatcgtagg gagcgctctg cacgccgagc
    14221 gccggcgcga tgacattgag gccgagacgc tcgtgcatcg actgcagcca ctcagcgtac
    14281 atgcccgggg cggagcgaag gcgaaagccg ttgaagaaga agagcgtcgc gtcgtgctcg
    14341 tgccggacga ggtagagcgg ctcgccgcgg tcgaggcaga acccggtgtg cgcatcgtag
    14401 gggacgccct cctcgaggcg cagggcccgg cggcgtacgg cacgctgcac ggcgagaccg
    14461 tacaggcgtt gtgcgccaat ggcggctagg ccagccaccg ccatgcgtcc ggcgagtcct
    14521 acgcccctgc gtcgcccggc cgccatggtt ccctctccgc gaggaaagcg agcacggcct
    14581 cgttgaaggc ctccggcgca tgctcgttcg cagcgtgtcc agccccggcg agcacgacct
    14641 tccgacagtt cggcatgcgc tccatcagcc aatcggatcg cacgacaaag tcgcggtcgc
    14701 gctcccccac gacgagcagc gtcgggcaac tgatgcgctc ggccttctcg agcgaggaga
    14761 tgtacggcaa tacctgagtc gccgtgtggg caatgcccgc gggcgagagc gcggcaaact
    14821 cgcggcgtct cgcctcgagc tcggcgggcg gcaggcgtcg attgatcatg ccatcggcga
    14881 gggccatgac gcccttcctc cgtagcgtct ccgccatgcc atgcatacgc ggctcgacct
    14941 gctcgcgcca ctcgtcaggg gcggcacccg aattcgagtt cacgacgacg aggcagtcta
    15001 tgcgatccgg gtgggcgagg gcgaagcgaa gagcgaccgc gccgccgagc gagtggccaa
    15061 cgacgtgcgc cctctcgatc ccaagggcat ccagcaaccc cttcgtgtcc tcgacgaaga
    15121 tgtccggcga gtacgcggcg ctgtcgtcgg gagcgctgct gcggccgtgg ccacgtagat
    15181 cgtaggcgat gaatcgatag cgctgggcga acggcgcgcg ctggaaactc cacatggcgc
    15241 aggtttccgt gaagccggga ataaagacga ccggaaagcc cgccccctct tcttcgtagt
    15301 acagctggat gccgttgacc gtagcatgag gcatgatcgc gcctcatta
//
LOCUS       DATMBB010000201         4537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_207333, whole genome shotgun sequence.
ACCESSION   DATMBB010000201 DATMBB010000000
VERSION     DATMBB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207333"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNL92_04400"
     CDS             complement(<1..222)
                     /locus_tag="VNL92_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWO93983.1"
                     /translation="MGDYEILAHVEMPECSIRIIGMEPTEAVELHYHRTFVQIYVILR
                     GEVEVRVGEQRLVLRPYETVRIAQETPHSV"
     gene            complement(228..848)
                     /locus_tag="VNL92_04405"
     CDS             complement(228..848)
                     /locus_tag="VNL92_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWO93984.1"
                     /translation="MSEPVAIVLAAGAGTRMGQPKALLPWGIGTLLGYQLRELAAAGF
                     AERIVVAGAAAPPVADITVKAGAALVVNTAWEEGRASSLRVGADAVRRDRDPILVLNV
                     DQPRPAPIHAGLLGAYRDEPAPIIVPVFQGRRGHPIILSGALLDELRTVRDEERGLRA
                     VVERHSSEVREIDLGDRLITIDINTPEEYEEARRIFEALAAEGTTT"
     gene            complement(845..1303)
                     /locus_tag="VNL92_04410"
     CDS             complement(845..1303)
                     /locus_tag="VNL92_04410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWO93985.1"
                     /translation="MTTVQVGDAAPDFTLESTEGPVRLSELRGNRVLLLFYQEDSTPT
                     CSNQLSAFIEDFETLQALGARVVAISVDDIDSHQRFAERLGGAPFPLATDPSLEVAQQ
                     YGVVDETGKRSRRAVFVLDKEGRVVLAIPWYSPSNFDQYEQIYRALGMNV"
     gene            complement(1322..2515)
                     /locus_tag="VNL92_04415"
     CDS             complement(1322..2515)
                     /locus_tag="VNL92_04415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWO93986.1"
                     /translation="MAEKLKVDPWDITAPRDAGGEPTLEELLRARIRTAGAMTFAEFM
                     ATALYEPRFGYYTTGVHRMGREGDYVTSPEVHPVFGALIAKQVVQCWELLGEPGSLRL
                     IEAGAGNGSLARDVLVALEALGLPPAIEYVIVEPFERVRRHQEDAILGTGRPEWNVRW
                     VAGLGEIEPGENVVVLSNELLDAFPVHRVVVRDGELREVCVGLEGDRFVEVEASVSDA
                     ALLDYFADLGVFPGEGCYAEVNLQAPAWITDVARLVARGFVLTFDYGATAEELYAPWR
                     RDGTLLCFWRQAPVHDPYARIGRQDMTASVDFTTLVRAGRKAGLEPLGLARQSEVLVA
                     LGIGDGIGDASGVLGLERYMERRRAATELLDPAGLGRILVLAQGRGVGRPHLLGFPEG
                     PPGLL"
     gene            2645..3106
                     /locus_tag="VNL92_04420"
     CDS             2645..3106
                     /locus_tag="VNL92_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWO93987.1"
                     /translation="MISDELIHRATQLYGRAASLVDPQRIGVWEELGLTIPQLRVLFF
                     LQREPNAPAGAIAQHLGVTPSTVTGLVDRLVRTRLVRRVEDPADRRMVRNTLTDRGRE
                     VVSELEQSGRAYMADIFRSMTPEQLENLVRALDDLVEAAARTQAEAVSSGA"
     gene            3169..>4537
                     /locus_tag="VNL92_04425"
     CDS             3169..>4537
                     /locus_tag="VNL92_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWO93988.1"
                     /translation="MQNLPARQKVVIMAGVLLGLFFSSMNQTVVSTAMPRIIASLGGI
                     DLYSWVFTSFMLTSTTTIPIFGKLSDIYGRKPLYLGGMVVFSIGSVLAGLSQDMTQLI
                     VFRAIQGLGSGGMMAISFTIIGDIFSPAERGKWQGLNSSVFGLSSVIGPLIGGYITDN
                     LSWNWVFYVNLPFGALAFAVIWFGLPWQRNPVKRPVDYFGVMALVGGLVPMLLAFVWG
                     GTEYAWSSPTILGLLAGSASMLIVFVIVEHRAEEPIMPLFLFRHRMFTVSVLATFVAG
                     IAMFATITYMPLFIQGVNSESATRSGIVMMPLMVSMMTASTLTGQIMSRTGKYLIPAL
                     VGTVIMGFGIFLASQMDVNSSNGEAMRNMVIIGLGLGPTMPVFIIAVQNAMPYRYMGV
                     VTSNVQFFRQIGGTMGVAIMGSVLNNRISTELDTRLPTEVQEQGSSELLGRLENPQVL
                     LDDSAL"
BASE COUNT          773 a         1493 c         1442 g          829 t
ORIGIN      
        1 aacgctatgg ggcgtctcct gggcgatgcg gacggtctcg tagggccgga ggacgagccg
       61 ctgctcgccg acccgcacct ccacttcgcc gcgcaggatg acgtagatct gcacgaatgt
      121 cctgtggtaa tgcagctcca cggcttcggt gggctccata ccgatgatgc ggatgctgca
      181 ttccggcatc tcgacgtgcg cgaggatttc gtagtccccc atgtgattca cgtggttgtg
      241 ccctccgctg ccagcgcctc gaagatgcgg cgcgcctcct cgtactcctc cggagtgttg
      301 atgtcgatcg tgatgagcct gtcgccgagg tcgatctcgc gtacttcgct cgagtgccgc
      361 tccacgaccg cgcgtagccc gcgttcctcg tcgcgaaccg tccgcaactc atcgagcaga
      421 gcgccggaca ggatgatcgg atggccacga cggccctgaa acacggggac gatgatgggc
      481 gcgggctcat cgcggtaggc ccccaggagg ccggcatgga tgggggccgg gcggggctgg
      541 tcgacgttga gcacgaggat cggatcgcga tctcggcgga cggcatcggc gccaacccgc
      601 agggaagacg cgcgtccctc ttcccatgcc gtgttcacga caagcgccgc gcccgccttg
      661 acggtgatgt cggcgacggg gggcgcggcg gcaccggcca cgacgatgcg ctcggcaaat
      721 cccgctgcgg ccagctcgcg cagttggtaa ccgaggagcg tcccgattcc ccaaggcagg
      781 agcgctttcg gctgccccat cctcgtgccg gcgcccgcgg ccaacacgat ggccaccggc
      841 tcgctcaaac gttcatcccc agggcgcggt agatctgttc gtactgatcg aagttcgagg
      901 gactgtacca ggggatagcc agcacgactc gcccctcctt atcgaggacg aagacagctc
      961 ggcgcgagcg cttgcccgtc tcgtcaacca cgccgtactg ctgggccact tcgagcgagg
     1021 ggtcggtggc caagggaaac ggggctccgc cgaggcgctc cgcaaaccgc tgatgggaat
     1081 cgatgtcgtc gacggatatc gcgacgacgc gcgccccaag cgcctgcagc gtctcgaaat
     1141 cctcgatgaa cgcggagagc tgattgctgc acgtcggcgt gctgtcttcc tggtagaaga
     1201 ggaggaggac gcggttgccg cggagctctg agaggcgtac cggcccctcg gtcgactcca
     1261 acgtgaagtc cggcgccgcg tcgcccacct gtaccgtcgt catcgtcccc tcccgcgaat
     1321 gtcagagcaa gcccggtgga ccctcgggaa agcccaggag gtggggtcgt cccacgccgc
     1381 ggccttgcgc cagcaccaga attcggccaa gccccgccgg gtcgagcagc tcggttgcag
     1441 cgcggcgacg ctccatgtag cgttcgagcc ccaacacccc gctggcgtcg ccgatgccgt
     1501 cgccgatccc gagagcgacg agcacctcgg actgacgtgc gagaccgagt ggctcgagtc
     1561 cggctttccg gccggcgcgg accagcgtcg tgaagtcgac ggacgcggtc atgtcctggc
     1621 gtccgatgcg ggcgtatggg tcgtggacgg gcgcctgccg ccaaaagcag aggagcgttc
     1681 cgtcgcgccg ccagggcgca tacagctcct ccgccgtcgc gccatagtcg aacgtgagga
     1741 cgaatccgcg cgctacgagt cttgccacgt ccgtgatcca cgccggtgcc tgaaggttga
     1801 cctccgcgta gcacccctct cccgggaaca cgccaaggtc ggcaaagtag tccaggaggg
     1861 ctgcatccga cacgctcgct tcgacctcga cgaatcggtc cccctcgagg ccgacgcaaa
     1921 cctcgcgcag ctcgccgtca cggacgacga cgcgatgcac tgggaacgcg tcgagcaact
     1981 cgttcgacag gacgacgacg ttctcgcccg gctcgatctc gccgagtccc gccacccacc
     2041 gcacattcca ctcggggcgc ccagtgccga ggatcgcatc ctcctggtgc cgtcgcacgc
     2101 gctcgaacgg ctccacgatc acgtactcga tggcaggagg gagcccgagg gcctcgagcg
     2161 cgaccagcac atcgcgcgcg agggagccgt tgcccgcgcc cgcttcgatg agccgcaacg
     2221 accctggctc gccgagcagc tcccagcact ggaccacttg cttcgcgatg agggctccga
     2281 acaccgggtg gacctccggg ctcgttacgt agtcgccttc gcgtcccatc cgatggacgc
     2341 cggtcgtgta gtagccgaac cgcggctcgt acagtgcggt ggccatgaac tcggcgaacg
     2401 tcattgcacc tgccgtccgg attctggcgc gaagcagctc ctccagcgtc ggttcgccac
     2461 cggcatcccg cggcgcggtg atgtcccagg gatcgacttt gagtttctcc gccatcgaaa
     2521 cccatgatag cgcagtttcg aaacgagtct aagataggaa gagcggcgag ctttgttggg
     2581 gttgcgacat tgacgccgcc gacgtactct tagctgagcg cgaacaagcg cgagggggga
     2641 gacgatgatc agcgatgagc tgattcaccg agcgacgcaa ctgtacggtc gagccgcgag
     2701 cctcgtcgat ccgcagcgca tcggcgtgtg ggaagagcta ggcctcacga ttccccagct
     2761 ccgggttctc ttcttccttc agcgggagcc caacgcgccg gcaggcgcca tcgcccagca
     2821 cctcggcgtc actccgtcca cggtgacggg gctggtcgac cgcctggtgc gtacgcggtt
     2881 ggtccgtcgt gtcgaggatc cggccgaccg gcgcatggtc cgcaacacgc taacggaccg
     2941 aggccgcgag gtggtttcgg aactggagca aagcggtcgt gcctacatgg ccgatatttt
     3001 ccgaagcatg acgccggagc agttggagaa cctcgtacgt gccctcgacg atctcgtcga
     3061 ggcagccgcg cgcacccaag ccgaggccgt ttcgtccggc gcctgatccc gatcggccgc
     3121 ccgttcaatc gacggccgat cgtctaagct cgttgcgaga agctcgctat gcagaacctt
     3181 cctgctcgcc agaaagtcgt catcatggcc ggcgttctgc tcggtctgtt cttctcgtcg
     3241 atgaaccaga ccgtcgtgag cacggccatg ccgcgcatta tcgcgagcct cgggggaatc
     3301 gacctgtaca gttgggtctt cacctccttc atgctcacct cgacaacgac catccccatt
     3361 ttcggaaagc tttcggacat ctacgggcgg aagccgctct acctcggagg gatggtcgtc
     3421 ttcagcatcg gatcggtgct tgccggcctg tctcaggaca tgacgcagct tattgtgttc
     3481 cgagcgattc aaggccttgg ctcgggcggc atgatggcga tttcgttcac catcatcggc
     3541 gacatctttt cgccggcgga gcgcggaaag tggcaaggcc ttaacagctc cgtcttcgga
     3601 ctttcgagcg tcatcggacc gctgatcggc gggtacatca cggacaatct ctcgtggaac
     3661 tgggtgttct acgtcaacct gccctttggg gcgctagcgt tcgcggtcat ctggttcggc
     3721 ctcccctggc agcgcaaccc ggtgaagcgc ccggtcgatt acttcggggt gatggcgctc
     3781 gtgggcggtc tcgtgccgat gctgctcgcg ttcgtctggg gtggcaccga gtatgcctgg
     3841 tcgtcgccga cgattctggg attgcttgcc ggttcggcat ccatgctcat cgtgttcgtc
     3901 atcgtcgagc acagagcaga agagccgatc atgcccctct ttctcttccg gcaccgcatg
     3961 ttcacggtgt ccgtgctggc gaccttcgta gccggcatcg cgatgttcgc gacgatcacg
     4021 tacatgcccc tcttcatcca gggcgtgaac agcgagtcgg cgacgaggag cggtattgtc
     4081 atgatgccgc tcatggtttc gatgatgacc gcctcgaccc tgaccggcca gatcatgtca
     4141 cgaacgggca agtatctcat cccagccctg gtcggcaccg tgatcatggg atttggcatc
     4201 ttcctggcca gccagatgga tgtcaattcg tcgaacggcg aggcaatgcg gaacatggtc
     4261 atcattggcc tcggccttgg gcccacgatg ccggtgttca tcatcgcagt gcaaaacgcc
     4321 atgccatacc ggtacatggg cgtcgtcacg tccaacgttc agttcttccg ccaaatcggc
     4381 ggcaccatgg gtgtggcgat catgggctcg gtactgaaca atcgaatcag caccgagctg
     4441 gacacgagac tgcccacgga ggtccaggag cagggctcgt ccgagctgct cggtcgcctc
     4501 gagaaccccc aggttctgct cgatgacagt gcgttgg
//
LOCUS       DATMBB010000202         5709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_288683, whole genome shotgun sequence.
ACCESSION   DATMBB010000202 DATMBB010000000
VERSION     DATMBB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288683"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VNL92_04430"
     CDS             <1..498
                     /locus_tag="VNL92_04430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWO93989.1"
                     /translation="VQNAGGTLHADGVTLFSGVRLEVGRGAQLSIGKGTFLNRNTTVV
                     CEREVTIGRDCMISWDVVILDTDQHERPGIGKGTAPVRIGDGAWICCRSVILKGVTIG
                     DGAVIGAGAIVTRDVPAHTLALGQPARLVRRLDTGETIHETNGHQPTFDNAHGWPASV
                     VERKS"
     gene            495..1367
                     /locus_tag="VNL92_04435"
     CDS             495..1367
                     /locus_tag="VNL92_04435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO93990.1"
                     /translation="MTTDARALRSAQPEAAAPGLLDYYVTSLLTGIRALPGRHVRDSL
                     ARIINPLSYPRYMEYRLALQALGDLGGMRVLDIGSPKLPALLIARDRSCELVATDIRD
                     YFVGSTAHFLRRNGLGDRIGSGVRLEVEDARHLSYGDATFDRVFSISVLEHIPDDGDS
                     EAMREIARVLRPGGTVAVTVPFDAAGYREDYAEGRVFEREDAAAPVFYQRHYDLATLT
                     SRLIAPSGLRVTDVTFFGEPGFRFEQYWNRIGMRLKLPLLWAQPFLAKLFLKELPPEN
                     VTAACGVALRLEKT"
     gene            1431..2567
                     /locus_tag="VNL92_04440"
     CDS             1431..2567
                     /locus_tag="VNL92_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWO93991.1"
                     /translation="MTEPRPPARQHDARPTSADHRTSVEPVEPRVEQATKPARDGYAT
                     GVPEIDQAIAALIATAAVAEGDLLREAITAAVRIARDGADRGEMKLIARTLREFGRAF
                     RLFAQYRDRPRVSIFGSARVQEHDPAYQTTARLARMLAERNWMVITGAGPGIMAAGNE
                     GAGAEASFGAGIVLPGFEQANIFIANDAKLLTFRYFFTRKVTFVKESDAFVLLPGGFG
                     TLDEAFELLTLMQTGKTAIRPVVLLEPPGSRYWRVWAEFVHDQLESRGFIASEDSAFY
                     RIAATPEEAAAEIESFYANYRSMRFVGRTLYLRLQVAPTAEQLAQLRSDFGDLLASSE
                     FEVVGPAPVEVADGDALDLARVAFEPVHNYARLRLLIDALNALG"
     gene            2634..3965
                     /gene="nhaA"
                     /locus_tag="VNL92_04445"
     CDS             2634..3965
                     /gene="nhaA"
                     /locus_tag="VNL92_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HWO93992.1"
                     /translation="MRGRVVRPIVEFLSTESAAGVVLVAATLVALVWANSPWNDTYTD
                     ILTTTITIDLGFVDVSEDVHHWVNDGLMTIFFFVVGLEIKRELLMGELNSVRRAALPV
                     AAALGGMVVPAALYLALNAGGEGEDGWGIPMATDIAFAVGLLALLGTRAPFALRVFLL
                     ALAIVDDLGAIAVIAVFYTDDLAVDALGIALGFIALILVMQRANVRDVPLYIPVGALL
                     WLALLKSGVHATIAGVILGILTPHLPFTTREEFTHRASEITSRLQGLLGRKREEETVD
                     EERYALQSLEDLTRDSESPLERLEHFLHPWSSFGIVPIFALANAGIELNADTISDALE
                     SEVMYGIVLGLVVGKTVGIFAASWLAVRSGMAELPAGVRWPQMLGVAMLAGVGFTVSL
                     FITELAFTDAELVGDAKIGILGASAIAGAAGFAYLWMVAERAPEVEPSLNR"
     gene            complement(3967..4269)
                     /locus_tag="VNL92_04450"
     CDS             complement(3967..4269)
                     /locus_tag="VNL92_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO93993.1"
                     /translation="MRLPRWPRFRKGSGRPSLDRRLAVQLMSMQAEPPGLLSLHPEDD
                     EPLREHLQLRAALLAQPKATPSIEAIAWGEERLLDELITRRCESRRRRRFLPPRRR"
     gene            complement(4266..4859)
                     /locus_tag="VNL92_04455"
     CDS             complement(4266..4859)
                     /locus_tag="VNL92_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWO93994.1"
                     /translation="MIDPIREHPEFAAPEDGLVEGLRAYNDDAWSELFALFHPLVYRY
                     FRAHLLDDDAAAEGSSSVFVEATRHIHRYRGDNDGLLPWLFDMAAAMLRAERGVSRER
                     RGRPRSHRDSRDERSAALITRLLRPAEDRTTTDPRAIRHALGSLRDDEREMILLRFSV
                     GLPPEQVARVLRISIDETYLREARALISLQRAVRALP"
     gene            complement(4988..5371)
                     /locus_tag="VNL92_04460"
     CDS             complement(4988..5371)
                     /locus_tag="VNL92_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWO93995.1"
                     /translation="MSMREDRLEKAQDVRRQLFGQVVPSAMPAGDVMTDFYDMINEWI
                     FGGVWARDGLPMKQRSMIVLSVLTALGREAELRLHLRAALNLGLTKEEISELLMQVAF
                     YAGVPASVSAFRIFREVLAQAEASS"
     gene            complement(5386..>5709)
                     /locus_tag="VNL92_04465"
     CDS             complement(5386..>5709)
                     /locus_tag="VNL92_04465"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWO93996.1"
                     /translation="RKAGVDVRQLAEIITKSSGNSLAINVFPRILDGQYGFSFTLDLA
                     TKDVSLAHQLARDVAAPARIAEMVEAVLKRGQAQGWGQEGCTVAIRILEQFSGVELGA
                     PEQGA"
BASE COUNT          965 a         1850 c         1859 g         1035 t
ORIGIN      
        1 gtccagaacg ctggcggcac attgcacgcg gatggcgtca ccctgttctc gggggtccga
       61 ctcgaggtgg gccgaggcgc gcaactctcc atcggcaagg gcaccttcct caaccggaac
      121 acgacggtcg tgtgtgagcg agaggtcacg atcgggcgtg actgcatgat ttcatgggat
      181 gtcgtgatcc tcgacacgga ccaacacgag cggcctggca tagggaaagg tacggcgccc
      241 gttcgtatcg gcgacggcgc atggatctgc tgtcgctcgg tcatcctcaa gggggtcacg
      301 ataggtgatg gcgccgtgat cggagcgggt gccatcgtta cccgcgacgt gcccgcccat
      361 accctcgccc tcgggcagcc cgctcgcctg gtccggcggc tcgatacggg cgagacgatc
      421 catgagacga acgggcacca gccgaccttc gacaacgcgc atggatggcc ggcttcggta
      481 gtggagcgca agtcatgacg accgacgcaa gggcgcttcg ttcagcacag cctgaagcgg
      541 cggcccccgg cttgctcgac tactacgtta cgagcctgct gacgggcatt cgcgccctgc
      601 ccggccgcca cgtgcgagac tcgttggccc gcatcatcaa cccactttcg tatcctcgct
      661 acatggagta ccgtctcgcg ctccaagctc tcggcgacct gggcgggatg cgggtgctcg
      721 acatcggcag cccgaagctc cctgcgctcc tcatcgcgcg cgaccgctcg tgcgagcttg
      781 tcgctaccga catccgcgac tacttcgtcg gctcgaccgc ccacttcctg cgccgcaacg
      841 gcctaggcga ccgtatcggc agcggcgtgc gcctggaggt agaggatgct cggcatctct
      901 cgtacggcga tgccacgttc gatcgggttt tctcgatatc cgtgctggag catatcccag
      961 acgacggcga tagcgaggcg atgcgcgaga tcgcccgggt gctccgaccg ggagggaccg
     1021 ttgccgtgac ggtgccgttc gatgcagcgg gttaccgcga ggattacgcg gaaggtcgtg
     1081 tgttcgagcg ggaagacgct gccgcgccgg tcttctatca acgccattac gacctcgcga
     1141 cgctcacctc acggctcatc gcgccgtccg gacttcgcgt taccgacgtc accttcttcg
     1201 gggagccggg ctttcgtttc gagcagtact ggaaccgcat cggcatgcgc ctcaagttgc
     1261 cgcttctgtg ggcgcagccg ttcttggcca agctgttcct gaaggagctg cctcccgaga
     1321 acgtcactgc cgcctgcggc gtggcactac gactcgagaa gacttaggca cactcggctc
     1381 ggccggcggc agtggcgacc ctgctctctt ctggcgcata atcgaagctg atgactgagc
     1441 ccaggccgcc agcgcgacag catgacgctc gccccacctc cgccgatcac cgaaccagcg
     1501 tcgagccggt cgaaccgaga gtcgagcagg ccacgaaacc ggcacgcgac ggttacgcaa
     1561 cgggcgttcc ggaaatcgac caggccatcg cggcgctgat tgcgacagcc gcagtcgccg
     1621 agggcgatct tctccgtgag gcgattaccg ccgccgttcg cattgctcgc gatggggcag
     1681 accggggtga gatgaagctc atcgcgcgga cactgcgcga gttcgggcgg gccttccgcc
     1741 tcttcgcaca gtaccgtgac cggccgcgcg tcagcatctt cggctcggct cgggttcaag
     1801 agcacgatcc cgcctatcaa acgacggcga ggctggcgcg catgctcgcg gagcgaaatt
     1861 ggatggtgat tacgggagca ggtcccggaa tcatggccgc cggcaacgag ggggctgggg
     1921 cggaagcgag cttcggcgcg ggtattgtcc tgccgggctt tgaacaagcg aacatcttca
     1981 tcgccaacga tgccaagctc ctcacgtttc ggtacttctt cacgcgcaag gtcacgttcg
     2041 tcaaggagtc ggatgccttc gtgctcctcc ccggcggttt cggcaccctc gacgaagcgt
     2101 tcgagttact cacgctcatg cagacgggga agacggccat tcggccggtt gtgcttctgg
     2161 agccgcctgg aagccggtac tggcgtgtgt gggcggagtt cgtccacgac caactcgaat
     2221 cgcgcggatt catcgcctcg gaggacagcg cgttctaccg aatcgcagcg acacccgagg
     2281 aagctgcggc cgagatcgaa tcgttctacg cgaactaccg ctcgatgcgg ttcgtcggca
     2341 ggacgctgta cctgcgtctg caagttgcgc cgaccgcgga gcagctcgcg caactgcgat
     2401 cggacttcgg ggacctgctc gcgtccagtg agttcgaggt cgtcggtccg gctccggtcg
     2461 aggtcgccga cggcgatgcc ctggacctgg ctcgcgttgc attcgagccg gtccacaact
     2521 acgctcgact gcggctgctc atcgatgcgc tcaacgcgct aggttagcag gccgatcaac
     2581 gactctgaac cagttccgcg atgagagcgg agactcagca caaggagcat gctttgcgcg
     2641 gacgcgtcgt acggccgata gtcgaatttc tcagcacgga atccgctgct ggcgttgtcc
     2701 tcgtcgccgc gacacttgtc gcgcttgttt gggcgaactc gccctggaac gacacgtaca
     2761 cggatatcct cacgacgacg atcacgatcg acctcggctt cgttgacgtt tcggaggacg
     2821 tgcaccattg ggtcaatgat ggcctgatga ctatcttctt cttcgtcgtc ggcctcgaga
     2881 tcaagcgcga gttgctcatg ggcgagttga acagcgttcg gcgcgccgcc ttgcccgtgg
     2941 ccgccgctct cgggggaatg gtcgttcctg cggcgctgta cctcgccctg aacgccgggg
     3001 gcgagggaga ggacggttgg ggcatcccga tggcgacgga tatagcgttt gccgtcgggc
     3061 tcctcgcgtt gctgggaacc cgtgcgccat tcgccctgcg cgtcttcctt ctcgccctcg
     3121 ccatcgtcga cgacctcggc gctatcgcgg tcatcgccgt cttctacacg gacgacctcg
     3181 cggtcgacgc tcttggaatc gcgctgggct tcatcgcgct cattctcgtc atgcagcggg
     3241 ctaatgtgcg tgacgtcccg ctgtacatac cggtcggagc actgctctgg ctggcactgc
     3301 tgaagtccgg ggtgcacgcg acaatcgctg gcgtgatcct gggtatactt acgccccacc
     3361 tcccgtttac gacgcgcgaa gagttcaccc accgcgcctc cgagataacg tcccgcctgc
     3421 aggggctgct cggccgcaag agggaggagg agaccgtcga cgaggagcgc tacgcgctcc
     3481 agtcgctcga ggacctgaca cgcgattctg aatcgccgct ggagcggctc gagcacttcc
     3541 tgcacccgtg gtcgagcttc ggcatcgtac cgatcttcgc tctggcaaat gccggcatcg
     3601 agctgaacgc ggacacgatt tccgatgccc tcgaaagtga ggtgatgtat ggaatcgtgc
     3661 tgggtctggt tgtggggaag actgtgggca tctttgccgc gagctggctc gccgtgcgaa
     3721 gcggcatggc ggagctgccc gcgggcgtgc gatggcctca gatgctggga gtcgcgatgc
     3781 tcgccggcgt cgggttcacc gtctcgctgt tcatcaccga gctcgccttc accgatgccg
     3841 aactcgtggg cgatgcgaag atcggtatcc tgggagcctc cgcgatagct ggtgcggcgg
     3901 gattcgcgta cctgtggatg gtggccgagc gcgcgcccga agtcgagcca tccttgaatc
     3961 gatgatctat cgtcgccggg gagggaggaa gcgacgtcgc cttcggctct cgcagcggcg
     4021 cgtgatcagc tcgtcgagga ggcgctcctc gccccaggcg attgcttcga tcgacggcgt
     4081 agctttcggc tgcgccagca acgcggcgcg caactggagg tgctcgcgca ggggctcgtc
     4141 gtcctcgggg tggagtgaga gcaaaccggg cggctccgcc tgcatggaca tgagttgcac
     4201 ggcgagccgg cggtcaaggc tgggccggcc ggagcccttg cggaagcgcg gccatcgcgg
     4261 aagcctcatg gcagagcccg gaccgctcgt tgcaggctga tgagcgcgcg cgcttcgcgc
     4321 aggtacgttt cgtcgatgga gatgcggagg acacgcgcca cctgctccgg cggcagaccc
     4381 acggagaacc gaagaagaat catctcgcgc tcgtcgtcgc gaaggctgcc gagcgcatgc
     4441 cgaatggcgc gagggtcggt cgtcgttcgg tcctcggccg gtcgcagcag tcgcgtgatc
     4501 agcgcggcac tgcgctcgtc ccgggagtcg cggtgagacc gcgggcgtcc acggcgttcg
     4561 cgcgacaccc cgcgctcggc cctcagcatg gcagcggcca tatcgaacag ccacggcagc
     4621 aagccatcgt tatcacctcg gtagcgatgg atgtgacgcg ttgcttccac gaagaccgag
     4681 ctagagccct ccgcggccgc atcgtcgtcg agcaggtgag cgcggaagta gcggtaaacg
     4741 agcggatgaa agagcgcgaa caactccgac cacgcgtcat cgttgtaggc gcgcaggccc
     4801 tcgacaaggc catcctccgg agcagcgaac tcgggatgct cacgaatcgg gtcgatcatg
     4861 acaatgccgc gaacccccgc caccggtccg gcgggcggga gtgcctccta gctcgattat
     4921 cggaggctag gggcaaaacc ttaggggaag gtccgagggg aagcagtgcc ccgctgacgc
     4981 gcaggcctca gctactcgcc tcggcctgtg ccagcacctc gcggaagatg cgaaacgctg
     5041 agacgctcgc cggcacgccc gcgtagaacg ctacctgcat cagcagctcc gagatctcct
     5101 ctttggtgag gcccagattc agggccgccc gcaggtgaag gcgcagctcc gcctcgcgac
     5161 caagtgccgt gagcacggag agcacgatca tgctgcgctg cttcatcgga aggccgtctc
     5221 ttgcccagac accgccgaag atccattcgt tgatcatgtc atagaagtcg gtcatgacat
     5281 cgcccgcggg catcgccgag ggaacgacct gaccaaagag ctgccgtcgg acgtcctgcg
     5341 ccttctcaag gcgatcttcg cgcatagaca tggctttctc ccccactagg ctccctgctc
     5401 aggggcgccg agctcgactc ccgagaactg ctcgaggatg cggatggcga cggtgcatcc
     5461 ctcctggccc catccctgcg cctgacctcg ctttagcacg gcctccacca tctctgcgat
     5521 gcgtgccggc gccgcgacat cgcgcgcgag ctgatgggcc aggctcacat ccttggtggc
     5581 gaggtcaagc gtgaagctga atccgtactg tccatcgagg atccgcggga agacgttgat
     5641 ggccagactg ttcccgctgc tcttcgtgat gatttcggca agctgtcgca catcgacacc
     5701 cgccttgcg
//
LOCUS       DATMBB010000203         2131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_579703, whole genome shotgun sequence.
ACCESSION   DATMBB010000203 DATMBB010000000
VERSION     DATMBB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579703"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1902
                     /locus_tag="VNL92_04470"
     CDS             <1..1902
                     /locus_tag="VNL92_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWO93997.1"
                     /translation="CFYYRSSRSFTPSDLRLATILADFAATAISSAEAYERVNVLRAV
                     AEESARVVQSVQRITDAALSKLGLQDLLHELLRRVRETLKADVATILLTSESGTELTM
                     SVHEGLDRTWEGPVRMPVGRGIAGTIASTLRATVIDDISKVEVVSEFLRRTVKSLAGV
                     PLLVEGRLIGVLHVATVKPRHFSDQDVQLLQLVGDRVGLAIEQARLFEAEREVRIQAE
                     QARETVAFIAESTRSLSASLNVDETLKRLANLAVPALADLCLIDVVDESGRIRRVTAS
                     VSEAHGPILRELEERYPLQDAPRGVMHVITTGETLFVPVVDVSRLSEVAQDARHLELL
                     WQIGPRSYIIVPLITRGRTIGALSVASIEAARTYREEDVIIVEEVARRAAIAIENARL
                     YRAAQEAARQREEFLSIASHELKTPLTTVKGYVQMLERELQRTDLSNDRVRELFGELQ
                     GQVRRFEDLVLDLLDASRLQRSRLDLQPEQADLVAILRSVMTRMERSTEFLPSHRLIA
                     EMPESIEGTWDAARLDQVFTNLIANALKYSPQGGDVRVSARQHDGVVEVTVRDSGIGI
                     AEDDFGAIFEPFVRGKVGRRGISGTGLGLYVARQIVEQHGGTISVESALGKGSTFIVR
                     LPMVANTDS"
     gene            complement(1899..>2131)
                     /locus_tag="VNL92_04475"
     CDS             complement(1899..>2131)
                     /locus_tag="VNL92_04475"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWO93998.1"
                     /translation="FALLRGDDPGEYVSHSVWQSREAFEAWTQSESFLKGHRQGSLHG
                     LLPGPPHLKVYEAVLVERGVVDGMDPASRGGR"
BASE COUNT          370 a          712 c          674 g          375 t
ORIGIN      
        1 tgcttctact atcgatcgtc ccgttcattc acgccgtcgg accttcgcct ggccacgatc
       61 ctcgccgatt tcgcggcgac cgcgattagc agcgccgagg cctacgagcg cgtcaacgtc
      121 cttcgggctg tcgcggagga atcggcccgt gtcgtgcaat cggtgcagcg gatcactgac
      181 gcggcgctct ccaagcttgg tttgcaggac cttctgcacg agctcttgcg ccgcgtccgc
      241 gagacgctga aggcggatgt cgccactatc cttctaacga gcgaaagcgg caccgagctc
      301 accatgagcg tgcatgaggg actcgatagg acgtgggaag gtcccgtgcg catgcccgtc
      361 ggccgtggaa tcgccggcac catcgcctcc acgctccgcg ctaccgtaat cgacgacatt
      421 tcgaaggtgg aagtcgtcag cgagttcctt cgccgcacgg tcaagtctct cgcgggtgtc
      481 ccgctgctcg tcgaagggcg ccttatcggg gtactgcacg tggcgacggt gaagccgcgt
      541 catttcagcg atcaggatgt ccagctcctt cagctggtcg gggaccgtgt cgggctcgcc
      601 atcgagcagg cgcggctgtt cgaggcggag cgcgaggtgc gtatccaggc ggagcaggca
      661 cgtgagacgg tcgccttcat cgccgagtcg acgaggtccc tatccgcctc gctcaatgtc
      721 gacgagacgc tcaagcgcct cgcaaatctg gccgtgccgg ccttggccga cctttgcctg
      781 atcgacgtcg tcgacgagag tggtcggata cgccgtgtca ccgcatccgt gtccgaggcc
      841 cacggtccga ttcttcgcga gctcgaggaa cgatatcccc tacaggacgc gccgcggggc
      901 gtcatgcacg tcatcaccac gggcgagaca ctcttcgtcc cagtcgtgga tgttagccgc
      961 ctttccgaag ttgcccagga cgcgcgccat ctcgaactgc tgtggcagat cgggccgcgc
     1021 tcctacatca tcgtcccgct catcacgcgt ggacgcacca tcggcgccct cagcgtcgcc
     1081 tccatcgaag cggcccgcac gtaccgtgag gaagatgtca ttatcgtcga ggaggtggcc
     1141 cgtcgggccg ccatcgccat cgaaaacgcg aggttgtatc gcgcggcgca ggaagcggcc
     1201 cgccagcgcg aggagttcct ttccatcgca tcgcacgagc tgaagacccc gctgacgacc
     1261 gtgaagggtt acgtccagat gctcgaacgg gaactgcagc ggactgacct gtcgaacgac
     1321 cgcgtccgtg agctcttcgg cgagttgcag gggcaggtgc gccgcttcga ggacctagtg
     1381 ctcgacctcc tcgacgcatc gcggcttcag cgaagccgcc tcgacctgca gccggagcaa
     1441 gccgatttgg tcgccatcct ccgcagcgtg atgacgagga tggagcggtc gacggagttc
     1501 ctccctagcc accggctcat cgccgagatg ccagaatcta tcgagggcac gtgggatgcg
     1561 gcgcggctcg accaggtgtt cacgaacctc atcgccaacg cgctcaagta ctcgcctcag
     1621 ggcggcgacg ttcgtgtcag cgcgagacag cacgatggcg ttgtcgaagt caccgtgcgg
     1681 gacagcggta tcgggattgc cgaggacgac ttcggcgcca tcttcgagcc gttcgtacgg
     1741 gggaaggtgg ggcgtcgagg catcagcggg accggcctcg gcctttacgt cgcccgccag
     1801 atcgtcgagc agcacggcgg caccatcagc gtcgaaagcg cgctcggcaa aggcagcacc
     1861 ttcatcgtgc gccttccgat ggtcgccaat accgactcct aacgtccgcc acgcgacgcc
     1921 gggtccatgc catcaacaac gccgcgctcc accaggacag cttcgtatac cttcaggtgc
     1981 ggcggccctg gaagcaggcc gtgcagcgag ccctgccggt gccccttcag gaatgactcg
     2041 ctttgcgtcc aagcctcgaa ggcctcgcgc gattgccaaa cggagtgcga cacgtactcg
     2101 cccggatcgt cgccccgcag cagggcgaac t
//
LOCUS       DATMBB010000204         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_45254, whole genome shotgun sequence.
ACCESSION   DATMBB010000204 DATMBB010000000
VERSION     DATMBB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45254"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VNL92_04480"
     CDS             <1..712
                     /locus_tag="VNL92_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO93999.1"
                     /translation="GRATSRLFAREGAKLVTADVTGAQEQTANEIRDAGGEATAVHVD
                     VTNESDVKAMLDKARQTYGRIDILYNNAGVLGELGALTDCTEQNFDRIISTNLKGVFF
                     GLKHGLRAIAESGGGAIVNTASIAGLVGLPGLGAYCASKGGVIQLTRTAALEGAAMNV
                     RVNCVCPGGVNTPMVQNAGLGRAAGDAGGAAAGATLGATRIIDPEEIGRLVLFLASDD
                     ASAITGGAYPIDLGVTAR"
     gene            complement(748..1626)
                     /locus_tag="VNL92_04485"
     CDS             complement(748..1626)
                     /locus_tag="VNL92_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anchored repeat-type ABC transporter permease
                     subunit"
                     /protein_id="HWO94000.1"
                     /translation="MSSFIDYLAEPWDYEFMRRAFLATAIVGAVAGLLGTYVVLRGLA
                     FIGDAIAHAAFPGVVIAFLLDRSLFLGGAIFGLATAVSIGAVSQTRRIREDTAIGVLF
                     AAAFALGVVLISTEDAYTGNLAAFLFGQVLAVSETDIALAAGFGAAIVVVALALQKEL
                     IAVAFDRDLAQSMGLPVFWLDLLLFAMVTMAIVISLRAVGNILVVAMLVTPAATARLL
                     TDRVYPMMALSTLLGAASGIIGLYISFHEDLAAGGLIVLVVTAFFALAWLFSPKYGIV
                     TGAARRFIGGRAQSDA"
     gene            complement(1610..2395)
                     /locus_tag="VNL92_04490"
     CDS             complement(1610..2395)
                     /locus_tag="VNL92_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWO94001.1"
                     /translation="MSVARTPRLQLSHVTVRYGDRTALEDVSMTVEPGSYVGVLGPNG
                     SGKTTLIRAILGSTPLSAGAVTVDGLPARSAKGVFAYVPQTHQIALDMPLTVWDVVLM
                     GRLPRLRWHRRYGRHDREVAAWALERVHLADERNRLIGELSGGQQQRAFIARALAQEG
                     RILLLDEPLTGVDAVTQEIVLRLLANLHADGLTILIATHDLSSAAGTCDTLCLLNRRL
                     VAYGPMRETFTPAALRETFGRHVHLGEGIEADHPELVEHVILH"
     gene            complement(2392..3330)
                     /locus_tag="VNL92_04495"
     CDS             complement(2392..3330)
                     /locus_tag="VNL92_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HWO94002.1"
                     /translation="MMRRSIIILPLLVFVLALAAACGDDDDDDAAGTSPASGEATETA
                     DAITVVATTTQIADMARNVADDRAEVESILTANTDPHEYEPEPATIRLIGDADLVLRN
                     GLELDAFLDDLLAEAGDAPVVTVTEGITQLSDDPHVWFDVARAKQMVENIRDALTQAD
                     PDGAEQYEANARDYLAQLDELDQYIRSETDTIPPECRKLVTDHEILTHFAAAYDLQII
                     GTIVPQGTTEAEPSAADLASLIDAIRVEGVPAVFGQASENRDLVEQVAGEAGISAVTD
                     LYLDALGPADSGASTYIDMMRHNTDLIVEALEGCAG"
     gene            complement(3367..3813)
                     /locus_tag="VNL92_04500"
     CDS             complement(3367..3813)
                     /locus_tag="VNL92_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWO94003.1"
                     /translation="MEQYVEHLQSRGRRATMARRAVIDTMAGLGEHFSAEELAQRVPH
                     IGRATVYRTLRLLQDEGLLCRVLLESGGLHYRLTERGHHHHLTCTECGAVQDFSDAEV
                     AGVMEGIARRAGFRMSGHWIEIYGMCGSCTQVAAAPASVAGQVAQA"
     gene            3955..>4180
                     /locus_tag="VNL92_04505"
     CDS             3955..>4180
                     /locus_tag="VNL92_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO94004.1"
                     /translation="MRFGLFYLIEKPEGASDLEVYQNTMEQIRVADELGYDYIWLAEH
                     RFTRYGIAPDVLVLGAWAAAVTKKVRIGTAV"
BASE COUNT          713 a         1333 c         1461 g          673 t
ORIGIN      
        1 cgggcgggcg acctcacggc tcttcgcgcg cgagggcgcg aagttggtta cggccgacgt
       61 gaccggcgct caggagcaga ccgccaacga gattcgcgat gcgggcggcg aggccacggc
      121 ggtccacgtc gatgtcacga atgagtccga cgtcaaggcc atgctcgaca aagcccgcca
      181 gacctatggt cgcatcgata tcctctacaa caacgccggc gtgctggggg agctgggcgc
      241 gctcaccgac tgcacggaac agaacttcga ccgcatcatc tcgacgaacc tgaagggtgt
      301 gttctttggg ctcaagcacg gcctgcgcgc catcgccgag agtggtggcg gtgccatcgt
      361 caacaccgca tccatcgcgg gcctcgtcgg gctgccggga ctcggcgcgt actgcgccag
      421 caagggcggc gtcatccagc tgacgaggac ggccgcgctc gaaggggcgg ccatgaacgt
      481 ccgcgtcaac tgcgtctgtc cggggggcgt gaacacgcct atggtgcaga atgcaggact
      541 cggtcgggcc gccggcgacg ccggcggcgc ggcggccggc gcgacgcttg gagcgacccg
      601 catcatcgat ccggaggaaa tcgggcgact cgtcctcttt cttgcgagcg acgatgcctc
      661 ggcaattacg ggcggagcgt acccgatcga tctcggcgtg accgctagat aaggcctgcg
      721 cggcctctcg ttagccctac ggcagcttca ggcgtcgctc tgcgcgcggc cgccgatgaa
      781 gcggcgggcc gcgcccgtga cgatgccgta cttcggcgag aagagccagg cgagggcgaa
      841 gaacgccgtg accaccagga cgatgaggcc cccggccgca aggtcctcgt ggaacgagat
      901 gtagagcccg atgatgccgc tcgcggcacc aagaagcgtc gacagcgcca tcatgggata
      961 gacacggtcc gtgagaagcc gcgcagtcgc ggccggcgtg acgagcatcg ctaccacgag
     1021 gatgttcccg acggcccgca acgagatgac gatggccatc gtcaccatgg caaagaggag
     1081 caagtcgagc cagaacacgg gcagccccat cgactgcgcg aggtccctat cgaacgctac
     1141 ggcgatgagc tccttctgga gagcgagcgc cacgaccacg atcgccgccc cgaagccggc
     1201 ggcgagggcg atatccgtct cggagacggc gagcacctgg ccgaacagga acgccgcgag
     1261 attgcctgta taggcatcct cggtactgat gaggacgacg cccagggcga acgccgccgc
     1321 aaagagcacg cctatcgccg tgtcctcgcg gatacgcctg gtctgcgaga ccgcgccgat
     1381 gctgaccgcc gtagcgaggc caaagatggc gcctcccaga aacaggctgc ggtcgagcag
     1441 gaaggcgatg accaccccgg gaaacgctgc gtgcgcgatc gcgtcgccga tgaaggcaag
     1501 gccgcgaagg acgacgtagg tgccgagcag ccccgcgacg gcgccgacga tcgccgttgc
     1561 gagaaaggct cgccgcatga actcgtagtc ccacggctcg gccaggtagt caatgaagga
     1621 tgacatgttc cacgagctcc ggatggtcgg cctcgatgcc ctcgccgaga tggacgtgac
     1681 gtccgaaggt ctcccgcaag gcggcgggcg tgaacgtctc ccgcatgggt ccgtacgcca
     1741 cgagccgtcg attgaggaga cagagcgtgt cgcaggtgcc ggcggcgctg ctcagatcgt
     1801 gcgtcgcgat gaggatcgtc aggccatcgg cgtgcaggtt ggcgaggaga cggaggacga
     1861 tctcctgcgt aacggcgtcg acgccggtca gcggctcgtc gagcaggagt atccgcccct
     1921 cctgggccag ggcccgggcg atgaaggctc gctgctgctg tcccccggag agctcgccga
     1981 tgagccggtt gcgttcgtcc gcgagatgga cccgctccag tgcccaggca gcgacctccc
     2041 ggtcgtgccg cccgtaccgg cggtgccatc gaaggcgggg caggcgtccc atgagcacga
     2101 cgtcccagac ggtcagcggc atgtcgagcg cgatctggtg ggtctggggc acgtaggcga
     2161 agacgccctt tgccgagcgg gcgggcaggc catcgacggt gacggccccg gcgctcaggg
     2221 gcgtggaacc aaggatggcc cggatgagcg tcgtcttgcc cgagccgttg ggtccgagca
     2281 cgccaacgta gctaccaggc tcgacggtca tcgacacgtc ttcgagtgcg gtgcggtcgc
     2341 cgtagcgcac ggtgacgtgc gagagctgga gtcgcggggt gcgtgcgacg ctcatccggc
     2401 gcagccctcc agcgcctcga cgatgaggtc ggtgttgtgc cgcatcatgt cgatgtacgt
     2461 gctggcgccg ctatcggccg ggccgagagc gtcgaggtag aggtcggtga ccgcactgat
     2521 ccccgcttcg cccgccacct gctcgacgag atccctgttt tccgatgcct gaccaaacac
     2581 cgccggtaca ccctcgacgc ggatcgcgtc gatgaggctc gcgaggtctg ctgcgctcgg
     2641 ctcggcttcc gtcgtgcctt gggggacgat cgtcccgatg atctgcaggt cgtaggcggc
     2701 cgcgaagtgg gtcaggatct cgtggtcggt gacgagcttg cggcactcgg gcgggatggt
     2761 gtctgtctcg ctgcggatgt actggtcaag ctcgtccaat tgcgccaggt agtcccgcgc
     2821 gttcgcctcg tactgctcgg cgccgtcagg gtccgcctgc gtcagggcgt cccggatgtt
     2881 ctcgaccatc tgcttggcac gggcgacgtc gaaccagacg tgtgggtcgt cgctgagctg
     2941 agtgatgccc tcggtgacgg tgaccacggg cgcatccccg gcctcggcca gcaggtcatc
     3001 gaggaacgcg tcgagctcga ggccgtttcg cagtacgagg tccgcgtcgc cgatgaggcg
     3061 gatcgtggct ggctccggtt cgtattcatg cgggtcggtg tttgccgtca ggatggactc
     3121 cacctcagcg cggtcgtccg ccacattgcg agccatgtcc gcgatttgcg tggtcgtggc
     3181 gacgaccgtg attgcatccg cagtttccgt ggcttctccc gacgcgggcg aagttccagc
     3241 agcgtcatcg tcgtcatcgt cgccgcaggc agcggcgagc gcgaggacga acacgaggag
     3301 gggaagaata atgatactac gtctcatcat cgactccaac aaaggcgcag ccctttgact
     3361 gcgccgctac gcctgggcga cctgccctgc gaccgatgcc ggtgccgccg ccacttgtgt
     3421 gcagctgccg cacatgccgt agatctcgat ccagtggcca ctcattcgga agccggccct
     3481 gcgggcgatg ccttccatga cgccggcgac ttcggcgtcg ctgaagtcct gcacagcacc
     3541 gcactcggtg cacgtgaggt gatggtggtg cccgcgctcc gtgaggcgat agtgcaggcc
     3601 accgctttcg agcaggaccc ggcacagcaa cccttcgtct tgcagcagac gaagggtccg
     3661 gtaaacggtg gcgcgtccga tgtggggcac ccgctgcgcc agctcctcgg cggagaagtg
     3721 ttcgccgagg ccggccatgg tgtcgatgac ggcgcgacgg gccatcgtgg cgcggcgtcc
     3781 ccgcgactgc agatgctcta cgtactgctc catacgagac accgtatcat gtagcgcctc
     3841 ggtccgcaat cggtgaagcc aacctgccgt ttgttgttga cacgccgcga gccgatgggc
     3901 agactgtcgg cggacagggg agactattgg cgctacgcgc cggggagatg agcgatgcga
     3961 tttggccttt tctaccttat cgagaagcct gaaggagcct cggatctgga ggtctatcag
     4021 aacacgatgg agcagatccg ggtcgccgac gagctggggt acgactacat ctggctcgcg
     4081 gagcaccggt tcacgcgcta cggcatcgcg cccgatgtgc tcgtgctggg cgcgtgggcc
     4141 gcggccgtca cgaagaaggt gcgcatcggc acggcggtgg
//
LOCUS       DATMBB010000205         3134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_397067, whole genome shotgun sequence.
ACCESSION   DATMBB010000205 DATMBB010000000
VERSION     DATMBB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397067"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VNL92_04510"
     CDS             <1..216
                     /locus_tag="VNL92_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94005.1"
                     /translation="NKFPDFVKRHRRFVGGATATAVISTTALVLAYRALGARMAKGES
                     PGEALKGVTEEEMDRSMALLRKIRRKK"
     gene            complement(155..1411)
                     /gene="alr"
                     /locus_tag="VNL92_04515"
     CDS             complement(155..1411)
                     /gene="alr"
                     /locus_tag="VNL92_04515"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWO94006.1"
                     /translation="MSEQVAPERAIIGQPPALWRGRPVWAEIHLDQLSENVRLLADRV
                     HPARLMAVVKANAYGHGAAPVAQAALEAGASYLGVVCVDEGVQLRAAGIEAPIVVLGY
                     TPVEQAEAVIENRLTPVVNSRQFALALSALASQFGVRCPVHVKVDTGMARFGCSPAEA
                     VDLAEWLRELPSIDVEGICTHFASADEEDKAFTQAQYERFLATTERLPWIPIRHAGAT
                     AVALDLPEMVLDMVRVGLGLYGYAPGETAPPVPIRPALALRSRIARIHTLEPGETVSY
                     GRTWRAERRTRVGLVMIGYGDGLPRLLSNRGEVLIRGRRASIIGRVCMDMTMVDLTDI
                     PEAQLEDEVTIIGAQGAERIGAQDLAVLCGTISYEILTGISARVPRLYLRGGRVVHAE
                     SLVATATTSYAGSSAAVPSTDPSPPR"
     gene            complement(1460..1942)
                     /locus_tag="VNL92_04520"
     CDS             complement(1460..1942)
                     /locus_tag="VNL92_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94007.1"
                     /translation="MNVMRFTVVDANGSVSFVFHGEALPALLASCAHDPRDIASLLAG
                     AEPYYPRLSEHVLNSLAVFDEHNAPGNPSVIHQAFDFLQPHELPAFRVIDERTREESL
                     RPVKAGAIVLNLLDRRIVQIQNTFHKIQRAGRWRVYQDSKPTERTFVYRIPRTWSLVP
                     "
     gene            2098..2235
                     /gene="rpmH"
                     /locus_tag="VNL92_04525"
     CDS             2098..2235
                     /gene="rpmH"
                     /locus_tag="VNL92_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWO94008.1"
                     /translation="MPKRTYQPKRIPRKREHGFRARMSTPGGRNVLRARREKGRKRLT
                     V"
     gene            complement(2244..2579)
                     /locus_tag="VNL92_04530"
     CDS             complement(2244..2579)
                     /locus_tag="VNL92_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94009.1"
                     /translation="MLRGLQAGPLDELRNGSLQVLEARLVCPRTSDHDDIPAIFNVEI
                     ASDGTKPPPHLVAYHGFPHSLANNEAKPRVRQVVSSNSNRKIGARPGPSGSVGSLKIA
                     AAAQPPMLR"
     gene            2594..2803
                     /gene="yidD"
                     /locus_tag="VNL92_04535"
     CDS             2594..2803
                     /gene="yidD"
                     /locus_tag="VNL92_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWO94010.1"
                     /translation="MRTAALASIRFYQRAISPWWPGQCRYFPTCSEYGREAIERYGVV
                     RGARLTVGRLLRCTPFRRGGYDPVP"
     gene            2830..>3134
                     /locus_tag="VNL92_04540"
     CDS             2830..>3134
                     /locus_tag="VNL92_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWO94011.1"
                     /translation="MKHPRALVVALAGLAIALVGCTGIDSPDGWAGAVVAGDVVYVND
                     EGDDLLAMSRADQAELWRFPGDNDDIDIEAIYGEPAVGDGRVFISGYDGFLYALDAET
                     "
BASE COUNT          562 a          943 c         1079 g          550 t
ORIGIN      
        1 aacaagttcc ccgacttcgt gaagcgtcac cggcgcttcg tgggaggagc gacggcgacg
       61 gcggtgatct cgacgacggc tctcgtgctc gcatatcgcg ccctcggcgc gcgcatggcg
      121 aagggcgagt ctccagggga ggcgctgaag ggcgttaccg aggaggagat ggatcggtcg
      181 atggcactgc tgcggaagat ccggcgtaag aagtagtagc agtcgcaacg agcgattccg
      241 cgtgcacgac tcgtcctcca cgaaggtaga gacgcggcac gcgggctgaa atcccggtaa
      301 gaatctcgta gctgatcgtt ccgcacagga ccgccaggtc ctgcgctccg attcgctcgg
      361 ccccctgggc gccgatgatc gtgacctcat cttcaagttg cgcctcggga atgtcggtga
      421 gatcgaccat cgtcatgtcc atgcaaaccc ggccgataat ggacgcgcgc cgtccgcgga
      481 tgaggacttc gcctcgattc gaaagcaggc gcggcaggcc gtccccgtag ccgatcatca
      541 cgaggccgac ccttgttcgg cgctctgccc gccacgtccg gccgtagctg acggtctcgc
      601 ccggctccaa tgtatgaatg cgggcgatcc ggctgcggag ggcgagcgcc gggcggatgg
      661 gaaccggtgg cgcggtctcg ccgggtgcgt acccgtacag gccaagcccc actcggacca
      721 tgtcgagcac catctcgggc agatcgaggg cgaccgccgt cgcgccggcg tggcgtatcg
      781 gaatccaggg cagccgttcg gtcgtggcga gaaagcgctc gtattgggct tgggtgaacg
      841 ccttgtcctc ttcgtcggcg cttgcgaagt gggtgcaaat gccctccaca tcgatcgagg
      901 gaagctcgcg cagccattcg gccaggtcca cggcttcggc cggcgaacag ccgaagcggg
      961 ccatgcccgt gtccaccttc acgtggacgg ggcagcgcac gccgaactgc gacgcgagcg
     1021 cggagagcgc gagtgcgaat tggcgcgagt tgacgaccgg cgtcaggcga ttctcgatca
     1081 cggcctcggc ctgttccacc ggcgtgtaac ctaagacgac gatcggcgct tcgatgcctg
     1141 ccgcgcgaag ctggacgccc tcatcgacgc agacgacgcc aagatagctg gcgccggcct
     1201 cgagcgccgc ttgagcgacg ggggccgcgc catgtccgta ggcgttcgcc ttcacgaccg
     1261 ccatcaggcg ggcggggtga acgcgatcgg cgaggaggcg aacgttctcg ctcagctggt
     1321 cgaggtgaat ctcggcccaa acagggcgcc cgcgccagag ggcggggggt tgcccgataa
     1381 tcgctcgctc cggggcgacc tgttcgctca tgacggaggg atcacgggtg accgcacgtc
     1441 cacatcgtcg gaggcaacct cagggaacga gcgaccatgt gcgagggatg cgatatacga
     1501 aggtgcgctc cgtgggtttc gagtcctggt acacgcgcca gcgacccgcc ctctgaatct
     1561 tgtggaacgt gttctgtatc tggacgatgc gtcgatcgag caggttgagg acgatggcgc
     1621 ccgctttgac tggacgcagg ctttcctcgc gggttcgctc gtcgatgacg cggaaggcgg
     1681 gcagttcgtg aggctggaga aagtcgaacg cttggtggat gaccgatggg ttgccgggcg
     1741 cgttgtgttc gtcgaacacg gcgaggctgt tcagcacgtg ctcggagaga cgcggatagt
     1801 acggctcggc gccggcgagc agcgacgcaa tgtcacgagg gtcgtgggcg cacgaagcga
     1861 gcaaggccgg caaggcctcg ccgtggaaca cgaagctcac ggaaccgttg gcatcgacga
     1921 cggtgaagcg catgacgttc atagcgtgcc ctcggactca gtttcacgac gattagtgta
     1981 gcacgatgcc attcctcgaa cactgaacgt acctggcggc cgaagctcgc tagtttgact
     2041 gcgtcaacgg gcgctaggta tactccgtgg gtatcgcgaa gtttccaggg aggccttgtg
     2101 cccaagcgaa cgtatcagcc gaagcgcatc ccgcggaagc gtgagcatgg attccgggct
     2161 cgcatgagca cgcctggtgg tcggaatgta ctccgggcgc gtcgcgagaa gggacgcaag
     2221 cgcctgaccg tctgaggccc ttgctagcga agcatcggcg gctgcgcagc cgccgcgatt
     2281 ttcagcgagc ctacgcttcc ggacgggcct ggacgggcac caatcttgcg attcgagttc
     2341 gacgaaacga cttgccgcac acgaggtttg gcttcgttgt tggcaagcga gtggggaaag
     2401 ccgtggtacg caacaaggtg cggcggcggc ttcgtgccat cgctcgcgat ctcgacattg
     2461 aagatggctg ggatgtcgtc gtgatcgctc gtccgggggc agacaaggcg ggcttcgagg
     2521 acctgcaacg atccattgcg cagctcatcg agcgggccgg cttgaagacc gcgcaacatg
     2581 ggtccgccac atcatgagga cagcggcgct ggcaagtatt cgattctatc agcgagccat
     2641 ctcaccgtgg tggcccggcc aatgccgcta cttcccgacg tgctcagaat acggacgcga
     2701 ggccatcgag cgatacggcg tggtacgagg cgctcgcctc acggtcggaa ggctccttcg
     2761 ctgcaccccg tttcgtaggg gtggatacga tcctgtcccg tgactgtttc acgtgaaaca
     2821 caaggacgaa tgaagcaccc tcgcgctctc gtcgttgcgc tcgcgggcct cgcgatagca
     2881 ctcgtcggct gcacgggcat cgattcgccg gatggctggg cgggcgcggt cgtggccggt
     2941 gacgtggtct acgtcaatga cgagggcgat gacctgctgg cgatgtctcg tgccgaccag
     3001 gctgaactct ggcgtttccc tggagacaac gacgacatcg acatcgaggc gatctacggt
     3061 gaaccggcgg ttggcgacgg ccgcgttttc atcagcgggt acgatggctt tctctatgcg
     3121 ctcgatgccg agac
//
LOCUS       DATMBB010000206         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_508132, whole genome shotgun sequence.
ACCESSION   DATMBB010000206 DATMBB010000000
VERSION     DATMBB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508132"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /gene="sat"
                     /locus_tag="VNL92_04545"
     CDS             <1..299
                     /gene="sat"
                     /locus_tag="VNL92_04545"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017250543.1"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HWO94012.1"
                     /translation="GNYYGTYDAQRIFSEFRPDELGITPLFFDHTFFCRSCDSMASVK
                     TCPHPEQYRVTLSGTQVRAMLSAGETPPIEFTRPEVARVLIEAQRAPAISEAGR"
     gene            296..1426
                     /locus_tag="VNL92_04550"
     CDS             296..1426
                     /locus_tag="VNL92_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94013.1"
                     /translation="MTVEEGRMAAPEATSQARRIWLPRPITRPTLTPLVLRRPVIAVA
                     AIWLFVIAIRTLSIGGAGIEPVLDALSVSNPIDALGFAWLGAYGVLSGSPIAAITMTL
                     FDGDLISRTEAFAMLNGSRFGASFIVLFVGFAAYVLRKREPDGIYIGVIALLTAFSLN
                     LPVLILGLGLLESGLLDGVQFDLPALVSIVETVADPAAHRIDDALPVGLVFVVGVGML
                     LTSFQMFDRALPQLEAADERFERLVVFLHRRWAMFAVGFLMTAMTLSVSVSLTVLIPL
                     SLKGYVRRDGVVPYVMGANISTWIDTLVASLLLGNPDAFTIVLAQMIVGLAVSLLILG
                     LLYRPYESALLGLSRRIVASRRSLATFLAIILIVPTILLVAS"
     gene            1453..2160
                     /locus_tag="VNL92_04555"
     CDS             1453..2160
                     /locus_tag="VNL92_04555"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWO94014.1"
                     /translation="MVTLSERFTPERLSELSAELEGQPPQDILRWAFETFHDKITLAC
                     SFGGASGMALLDMAVKLRPDVRVFYLDTDFLFPETYETVRKASERYRIEPIALRPLLS
                     VAEQAERHGEALWQRDPDACCAIRKVEPNRRALEGMEAWISGLRRDQAETRKQIAVVS
                     WDAKFGVVKVSPLAAWTEKDVWRYIVENDVPYNPLHDRGYPSLGCTHCTRPVQPGEDA
                     RAGRWSDFDKTECGLHD"
     gene            2178..2747
                     /gene="cysC"
                     /locus_tag="VNL92_04560"
     CDS             2178..2747
                     /gene="cysC"
                     /locus_tag="VNL92_04560"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWO94015.1"
                     /translation="MPGGAEHLERGFTVWFTGLSGAGKTTIAERVQAELRDRGHRVEV
                     LDGDVVRTHLSKGLGFSKEDRDTNIRRIAFVAHLLTRNGVAVITAAISPYRDVRSEAR
                     EMIGDFVEVYVKCPIEVLAERDVKGLYKKALAGEIQNFTGVSDPYEEPHAPEVIVETD
                     REAVEDSVAKVLRALEELGYTRSRSTIAP"
BASE COUNT          491 a          955 c          901 g          545 t
ORIGIN      
        1 tggggaacta ctacggaacc tacgacgccc agcgcatctt ctcggaattc cggccggacg
       61 agctgggtat cactcccctc ttcttcgacc acaccttctt ctgccgctcg tgcgacagca
      121 tggcgagcgt caagacgtgc cctcacccgg aacagtatcg cgtcacgctg tccggcacgc
      181 aggtgcgggc gatgctgagc gcgggggaga ctccgccgat cgagttcacg cggccggagg
      241 tcgcgcgcgt gctgatcgag gcccagcgag cgccggcaat atccgaagcg gggcgatgac
      301 cgtcgaggag gggcggatgg cggcgccgga ggcgacttct caagcccgcc gcatctggct
      361 accgcggccg atcacacgcc caaccctgac tccgctcgtt ctgcgccgcc cggtcatagc
      421 cgtggccgcc atctggctgt tcgtcatcgc tatccgcacg ctgagcatag gtggcgcggg
      481 tatcgagccg gtactcgacg ccctctccgt ttccaatcca atcgatgcgc tgggctttgc
      541 ctggttgggc gcatacggcg tcctgagcgg ctccccaatc gctgccatca ccatgaccct
      601 gttcgatggc gacctgattt cgcggaccga ggccttcgcg atgctgaacg gctctcgatt
      661 cggggcctcg ttcatcgtcc tgttcgtcgg attcgccgcc tacgtgcttc gcaagcgcga
      721 gcccgatggc atctacatcg gcgttatcgc gctgctcacc gcgttctcgc tcaacctgcc
      781 cgtgctgatc ctcggcctcg ggctgctcga gtcgggcctg ctcgatggcg tgcaattcga
      841 cctgccggcg ctcgtgtcca tcgtcgagac ggtggccgat ccggccgcgc accgcatcga
      901 cgatgcccta ccggtaggcc ttgttttcgt ggtgggcgtg ggcatgctgc ttacgagctt
      961 ccagatgttc gatcgcgccc ttccgcagct ggaggcggcc gacgaacggt tcgagcgcct
     1021 cgtcgtcttt ctgcaccgtc gctgggccat gttcgccgtc ggattcctga tgacggcgat
     1081 gacgctctcc gtttcggtgt cgctcaccgt gctcattccc ctttccctca agggatacgt
     1141 gcggcgcgat ggcgtcgtgc cgtacgtcat gggggcgaac atctcgacct ggatcgatac
     1201 cctcgtagcg agcctcctcc tcggtaaccc agacgccttt actatcgtgc tggcgcagat
     1261 gatcgtaggc ttggcagtct cgctcctcat tctgggcctg ctgtaccgcc cgtacgaatc
     1321 ggcgcttctt ggcctcagcc ggcgcatcgt cgcgtcgcgc cgcagcctgg cgacgtttct
     1381 cgccatcatc ctgatcgtgc ccacgatact gctggtggca tcatgacgga gcgtaatcaa
     1441 caggaggaag cgatggtcac gctgtccgag cggttcacac cggaacggct gtccgaactg
     1501 tctgcagagc tggaaggcca gccgccgcag gacatcctgc ggtgggcctt cgaaacattc
     1561 cacgacaaga ttacgctcgc ctgcagcttc ggcggggctt ccggcatggc gctactcgat
     1621 atggctgtga agctccgccc ggatgttcgc gtgttctacc tcgataccga cttcctcttt
     1681 cccgagacct acgagacggt ccggaaggcc tcggagcggt accgaatcga accgatcgcg
     1741 cttcgcccgc tcctcagcgt tgccgagcaa gccgagcgtc acggcgaggc gctgtggcaa
     1801 cgcgatcccg acgcctgttg cgcgatccgc aaggtggagc ccaaccgccg ggcgctcgag
     1861 gggatggaag cgtggatctc cggactgcga cgcgaccagg cggagacgcg caaacagata
     1921 gccgtcgtca gctgggatgc gaagttcggc gtagtcaagg tgagtcctct cgcagcgtgg
     1981 acggagaagg acgtgtggcg ctacatcgtc gagaacgacg ttccctacaa cccgctacac
     2041 gaccgaggct accccagcct cggctgcacg cactgcacac gtccggtgca gcccggcgag
     2101 gacgcccgtg cgggccggtg gtccgacttc gacaagactg agtgcggcct gcacgactga
     2161 ttcgcgtatc acgaacgatg ccaggaggag cagaacactt ggaacgtgga ttcactgtct
     2221 ggttcacagg cctctcggga gcgggaaaga ccaccatcgc cgaacgcgtc caagcggagc
     2281 tgcgcgaccg cggccataga gtcgaggtgc tcgacggcga cgttgtgcgc acccatctga
     2341 gcaaggggct cggcttcagc aaggaagacc gggacacgaa catccgccgt atcgctttcg
     2401 tggcccacct gctcacgcgc aacggcgtcg ccgtcatcac cgcagccatc tcgccctatc
     2461 gcgacgtacg tagcgaggcg cgcgagatga tcggcgactt cgtggaggtg tacgtgaaat
     2521 gccccatcga agtgctcgcc gagcgtgacg taaagggcct ctacaagaag gccctcgccg
     2581 gcgagattca gaacttcacc ggcgtgtccg acccgtacga ggagccgcac gcgccggagg
     2641 taatcgtcga aaccgaccgc gaagcagtgg aggatagcgt ggccaaggtg ctccgcgcac
     2701 tcgaggagct gggctacaca cgctcccgct cgacgattgc cccctaactc aagatgccgt
     2761 tgggacgcgc cgcttgtgaa gctacggcgg tggtgctacg attgttgaca gaatcactct
     2821 actttctctg aggtgagcgt gcgcgtctcc accaaggtcg attacggtat ccgcgctctc
     2881 atcgagctcg cc
//
LOCUS       DATMBB010000207         2426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_511661, whole genome shotgun sequence.
ACCESSION   DATMBB010000207 DATMBB010000000
VERSION     DATMBB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511661"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..462
                     /locus_tag="VNL92_04565"
     CDS             235..462
                     /locus_tag="VNL92_04565"
                     /inference="COORDINATES: protein motif:HMM:NF027617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C2H2 type zinc finger domain-containing protein"
                     /protein_id="HWO94016.1"
                     /translation="MTRDLSCPVCLMTFREGDYHALAAHVSQEADRSDGKHIMWLNRN
                     VTKLRTDPSTLAELLKRYLEGGTTGAERIVR"
     gene            480..1217
                     /locus_tag="VNL92_04570"
     CDS             480..1217
                     /locus_tag="VNL92_04570"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HWO94017.1"
                     /translation="MTSERERAERIERLVVDEYFRNPPPFVRAMASEPTLGVARAFVI
                     EWTKFSLQFPRWVGQIVANCPEFPVIAFEIENLMSEVVRDPAADTNHYELLIRLGDAL
                     GLSRSEIESHQPSDEAKRAFSYWWDAARQPDWLLGFAAINGLEILGDRTLPRRHGLSQ
                     GTGLAVHPWTRLGLPEAALEFFRVSDEADEGHGRETVEILARYAHDDERMSQAMEALR
                     RTIGELRSMMDGMQRVAEAMETRAAAG"
     gene            1347..1514
                     /locus_tag="VNL92_04575"
     CDS             1347..1514
                     /locus_tag="VNL92_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94018.1"
                     /translation="MAHLRERIGEGVIVRSDDVVGNDILGEATWHGRRYRVRIRCNDD
                     GTVAGSDIEPA"
     regulatory      complement(1784..1923)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            2089..>2426
                     /locus_tag="VNL92_04580"
     CDS             2089..>2426
                     /locus_tag="VNL92_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWO94019.1"
                     /translation="MPRSIWTGAISFGMVSIPVKLYAATESKDVTFHLLHDECKTRVK
                     QLRWCPVHEREVGWDELVRGYEYAKGQYVIVTEEDFESLPVPSKHVIDLSAFVQAEEI
                     DPIYYERSYYL"
BASE COUNT          453 a          740 c          808 g          425 t
ORIGIN      
        1 gccggaaggc ttgtcgccga tggcggcaaa ggccgcgaac tgtgtcccat cggccgaggt
       61 caacgtaagg tcgcggtgtt cgacggccgc gcccgcgatc ggcgcgatcg gaggtcgcgc
      121 gtccggtgaa tagcacatag cttgctctct cctccccttc ctgaatacaa gcggcggcat
      181 tgtagcatgc acatcgaggc ggcgatccct tagcgctcgc gataagatgg tgtcatgact
      241 cgcgatctga gttgccccgt ctgcctgatg actttccggg agggcgatta ccacgcgctc
      301 gccgctcacg tttcacagga ggcggaccgc agcgatggca agcacatcat gtggctcaat
      361 cgcaacgtca ccaagctcag gaccgatccc tcgacactcg ccgagctcct gaagcgatat
      421 ctggaaggtg ggacgacggg cgccgagcgc atcgtccgct gagccaaagg aggtgcaaca
      481 tgacaagcga gcgtgagcga gcggagcgca tcgagcggct ggtcgtcgac gaatactttc
      541 gcaacccgcc gccgttcgtg cgcgccatgg cgtcggagcc cacactgggc gtggcgcggg
      601 ccttcgtgat cgagtggacg aagttctcgc tgcagttccc gcgctgggtg gggcaaatcg
      661 tcgccaactg cccggagttc cccgtcatcg cgttcgagat cgagaatctg atgtcggaag
      721 tcgtgcgcga tccagcggcg gacacgaacc actacgagct gctcatccgc ctcggcgatg
      781 ccttaggcct gagccgatct gagatcgagt cgcatcagcc gagcgacgag gccaagcggg
      841 cgttctcgta ctggtgggat gcggcgcgtc agcccgattg gctgctgggc ttcgccgcca
      901 tcaacggact cgagatcctc ggcgatcgga cgttgccgcg gcgccacggc ctctcccagg
      961 ggacaggcct tgccgtgcat ccgtggacca ggctcggcct gccggaggcg gctctcgagt
     1021 tctttcgcgt gagcgatgag gctgacgagg gccatgggcg agagaccgtc gagatcctgg
     1081 cccgctacgc tcacgacgat gagcgcatgt cgcaagccat ggaggcgctg cgccggacga
     1141 tcggcgagtt gcgctcgatg atggatggca tgcaaagggt ggccgaggcg atggagacac
     1201 gagcagccgc cggctaggag gtacgaatgt cgcgagagga accgtgtgcc ctatgcggct
     1261 cgacctgggg cgactatcac gccgacatct tcgggcgcaa ctaccacttt tgctgaaacc
     1321 tctgagggga cgccttcgcc aacatcgtgg cgcacctgcg ggagcgcatc ggcgaaggcg
     1381 tgatcgtccg gtcggacgat gtggtaggca acgacattct cggcgaagcg acgtggcacg
     1441 ggcgccgcta tcgggtgcgg atacgctgca acgacgacgg caccgtcgcg ggctcggaca
     1501 tcgagccggc ctgatgcgcg cgaaccggga ccggccaggc taggccggca cgggtgtacg
     1561 ccggggccgg cgagatggtc gccgatcgct gacgccgttg ccgcacatgc gacagatagg
     1621 cgtccccgcg gacttctcat cctcgatgag gtcgccgagg cagattgggc aggggcgagg
     1681 aatcgaaaag tcgtcttcgg cttctgaact tcgagaactt gccttccgca cgcgtgccct
     1741 ccttcgcggg aacggccgcc gcatggacct atggacccgg cgcacggcct ctcagtggcc
     1801 tacggggtta gctgtcgggt tcggactgag aggtctgtcc gtcctgccga ggcaggattc
     1861 accccaattg ttcggttccc ccgttccccg cacctgcgtg cgaggactca gccgtattcg
     1921 ttgcaagtct aggcgagagg acgacgctcg tcaacgtaac aacattacaa gtttgtgacg
     1981 aacggcttcg cccgcccgca tatgcgacgc ggcgttaggt acgaacataa tatgatagga
     2041 tggtaatccg ccaagcgttg attggcgaaa tcaacgagga ggctgccggt gccgcgttcg
     2101 atctggacgg gggccatcag cttcggaatg gtcagcattc cggtcaagct gtacgcggcg
     2161 accgagagca aggacgtcac gttccacctg ctccacgacg aatgcaagac gcgcgtgaag
     2221 caactccgct ggtgccccgt ccacgagcgc gaggtgggtt gggatgagct cgtacggggc
     2281 tacgaatacg cgaagggcca atacgtcatc gtgacggagg aggacttcga gtcgttgcct
     2341 gtccccagca agcacgtcat cgacctttcg gcgttcgtgc aagccgaaga gatcgatccg
     2401 atctactacg agcgcagcta ctacct
//
LOCUS       DATMBB010000208         2410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_76902, whole genome shotgun sequence.
ACCESSION   DATMBB010000208 DATMBB010000000
VERSION     DATMBB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76902"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VNL92_04585"
     CDS             complement(<1..753)
                     /locus_tag="VNL92_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWO94020.1"
                     /translation="MAVVQSVVPFLLILLVLVVIHELGHFITAKLAGVSVLEFGIGYP
                     PKIWGKKFGETEYTINWLPLGGFVRLVGEEDPSDPRSLARRPRWIRLIVLASGSIMNL
                     LLPILLFAIAFMIPQEISIGRPIIADVVPDSPAAEAGLQPGDVIYEAEGRETRNLADL
                     SRAIQLNLGEQLDLKVKRGTEFVQLDAKARWVPPDGQGPLGIRIASQYPFTETESYPV
                     WEAVPKGFQATWETVILARNQIIALIKGGGESE"
     gene            complement(763..1917)
                     /locus_tag="VNL92_04590"
     CDS             complement(763..1917)
                     /locus_tag="VNL92_04590"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWO94021.1"
                     /translation="MGDSVTRVALLGSTGSIGTQALEVLRGLAPQYRVAGLAAGRNVA
                     LLARQIAESRPQLVSLEDARMMRSLPEGLPAFEPTTAIDMASHPDVDLVIVGTSGKAG
                     LAPILAALRAGKDVALANKEALVMAGGIIMDEARRTGRTVRPIDSEHSAIWQCLWGED
                     PSALRRIILTASGGAFRDLPLEALERVSPEEALRHPTWNMGPKITVDSATLMNKGMEV
                     IEAHWLFDVPLDRIDVILHRESIVHSMVEMHDGSVKAQLGWPDMRLPIRCALTYPARV
                     GAEAQPALDLTTVRSLTFDQIDRRRFPCLDVAIEAGSRGGTYPAAMAAADEVAVELFL
                     ARRLGFMDIASVVEEAMLHHPAVSAPSLDDILEVDEWARDYVARQATAHA"
     gene            complement(1902..>2410)
                     /locus_tag="VNL92_04595"
     CDS             complement(1902..>2410)
                     /locus_tag="VNL92_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HWO94022.1"
                     /translation="RTETALHDWAVVAAGALYIGWLGAHAVLLRELDDGREWVLLAFG
                     VVFATDTGAYFIGRAFGRRKMAPRVSPGKTWAGGVGGFACGAIVAAVLNFAFGLDAPP
                     WEIAGLAIIVPVTAEIGDLAESVLKRSMHVKDMGKVMPGHGGIVDRLDSLLFAIPVLY
                     YWVRWVIQ"
BASE COUNT          424 a          788 c          791 g          407 t
ORIGIN      
        1 ctccgattcg ccgccaccct tgatgagcgc gatgatctgg ttgcgcgcga ggatgacggt
       61 ctcccaggtc gcctggaatc ccttcggcac cgcctcccag acgggatacg actcggtctc
      121 cgtgaaggga tactgcgacg cgatgcgtat gccgagtggt ccttggccat ccggtggtac
      181 ccagcgggcc ttcgcatcga gctgcacgaa ctccgtgccc cgcttcacct tcagatcgag
      241 ctgctcgccc aggttgagct gaatggcgcg cgaaaggtcg gcgaggttgc gggtttcgcg
      301 gccttccgct tcgtagatga cgtcgcccgg ctggagcccg gcctcagccg ccggactgtc
      361 gggcacgacg tccgcgatga tgggccgtcc gatcgagatc tcctgcggaa tcatgaatgc
      421 gatcgcgaag aggaggatgg ggagcagtag gttcatgatc gaacccgatg ccagcacgat
      481 gaggcgaatc cagcgaggtc ggcgggcaag gctgcgcgga tcgctcggat cctcctcgcc
      541 cacgagccgg acgaagcccc cgagcgggag ccagttgatg gtgtactccg tctccccgaa
      601 cttcttcccc catatcttcg ggggatagcc tatgccgaac tccagcacgc tcacgcccgc
      661 gagcttcgcg gtgatgaagt ggccgagctc gtggatgacg acgagcacga ggaggatgag
      721 caggaacggg acgacggatt gaacgacggc catcgatagg tcctacgcgt gtgccgtcgc
      781 ttgtcgcgcc acgtagtcgc gcgcccactc gtcgacttcg agaatgtcgt cgaggctcgg
      841 cgcggagacg gccgggtggt gcagcatggc ttcctcaacg accgaggcga tgtccatgaa
      901 tcctagcctt cttgccaaga acagctcgac agcgacctcg tcggcggcgg ccatagccgc
      961 gggatacgtg ccccctcggc tgcccgcctc gattgcgaca tcgaggcaag ggaatcgccg
     1021 ccggtcgatc tggtcgaatg tcaacgagcg taccgtcgtc aggtcgagcg cgggttgagc
     1081 ctcagcgccg acccttgccg gataggtgag ggcgcaccgg atggggaggc gcatatccgg
     1141 ccagccgagt tgcgccttca ccgagccgtc gtgcatctcg accatcgagt gaacgatgct
     1201 ttcgcggtgt aggatcacgt cgattcggtc gagcggcaca tcgaagagcc agtgcgcctc
     1261 gatgacctcc atgcccttgt tcatgagcgt ggccgaatcg accgtgatct tcggtcccat
     1321 gttccaggtc gggtggcgca gcgcctcctc gggactcacg cgctctaacg cctcgagggg
     1381 caggtcacga aacgctccgc ccgatgcggt gaggatgatg cgtcgcaggg ccgatgggtc
     1441 ttcgccccac aggcactgcc aaatggcgct gtgctcgcta tcgatgggcc gaaccgtgcg
     1501 gccggtgcga cgtgcttcgt ccatgatgat gccgccggcc atcacgagcg cctctttgtt
     1561 tgccaacgcg acgtccttgc ccgcccgcag ggcggcgagg atgggcgcaa gtcctgcctt
     1621 gccgctggtc ccgacgatga cgagatccac atcaggatgg gacgccatgt cgatggccgt
     1681 tgtcggctcg aacgcgggca gaccctccgg gagagaccgc atcatacggg cgtcctcgag
     1741 gctcaccagt tggggtcggg attccgctat ctggcgggcg agcagcgcaa cgttgcggcc
     1801 cgcggcgagg cccgcaacgc gatactgcgg ggccaaaccc cgcagtacct cgagtgcttg
     1861 cgttccgatc gaaccggtgg agccgaggag cgccacgcgg gttactgaat cacccatcgc
     1921 acccagtagt atagcaccgg aatcgcgaac agcaggctat cgagccggtc gacgatgccg
     1981 ccgtggccag gcatcacctt gcccatgtcc tttacgtgca tcgaccgttt gagcaccgac
     2041 tccgcgaggt cgccgatctc ggcggtcacg gggacgatga tggcgagtcc ggcgatctcc
     2101 cacggaggag catcgaggcc aaacgcgaag ttcagcacgg cggcgacgat ggcgccgcag
     2161 gcgaaaccac ctacgcctcc cgcccaggtc ttgccggggc tcacgcgagg cgccatcttg
     2221 cgccgtccga acgcgcgccc gatgaagtag gcgccggtgt cggtggcgaa cacgacgccg
     2281 aaggcgagta acacccactc cctcccatcg tcgagctccc gaagcagcac ggcgtgagct
     2341 cccaaccagc cgatatacag ggcgcccgcc gcgacaacgg cccaatcgtg tagggccgtc
     2401 tccgttcgtg
//
LOCUS       DATMBB010000209        10763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_48377, whole genome shotgun sequence.
ACCESSION   DATMBB010000209 DATMBB010000000
VERSION     DATMBB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48377"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1217)
                     /gene="thrC"
                     /locus_tag="VNL92_04600"
     CDS             complement(147..1217)
                     /gene="thrC"
                     /locus_tag="VNL92_04600"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWO94023.1"
                     /translation="MRRALPRSEGVLARYAQYLPLTPATPMISLGEGATPLIRSRRIV
                     DRIGCRALYFKLESCNPTGSFKDRGMVVAVAKAIEDGARAVLCASTGNTSASAAAYAA
                     VCDLDCFVLVPKGRIAQGKLAQAVAYGARVLAIDGSFDSALELARAVTGDHNIALVNS
                     VNPNRIAGQKTAAFEIVDDLGEAPDLLCIPVGNAGNITAYWQGFEEYMEAGRTETAPR
                     MMGFQAAGAAPIVLGHPVDDPQTIASAIRIGNPASWRGAEIARDASGGAIEAVTDDDI
                     LEAYQLLSREEGIFAEPASAASLAGAMKLAREGRIEDTDVIVCIITGSGLKDPETALT
                     IPQNLIEVPAEREAIERALVEA"
     gene            complement(1192..2235)
                     /locus_tag="VNL92_04605"
     CDS             complement(1192..2235)
                     /locus_tag="VNL92_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94024.1"
                     /translation="MTRNRSASSYRDVEDLPAAFLDAVEWMQDQLADLREQIGRVRAD
                     AEQTNARLNTVHSQLDRIDTRLTRQEHTTNVAIHLQRALDEARAIVEDIRQRQLATER
                     DLATVMRDQDEHSERNADARAELQGRIDEVARLTVDLDSRTNVLDEAHRHVQDRLSAL
                     RQEVDELATTVATTQNRQGTVGEAIHRLDASHALLDSRSNSLQSSLEVLTERVQSAME
                     QARRVDLRIDTAVLPGLAAIDEIVEAIELLRAERSRIESAQQTASQRIDRVEEALAGQ
                     SSATARLDARANSFEERLSQAQTELEEVRERVVQQVLRLIQTEERQKRREIDDLEREI
                     RELKQYATRLAEE"
     gene            complement(2232..3527)
                     /locus_tag="VNL92_04610"
     CDS             complement(2232..3527)
                     /locus_tag="VNL92_04610"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWO94025.1"
                     /translation="MTRNAIGVGLLGLGTVGGAVAEAITSREQLYAERAGLPLTLRRA
                     VVRDLAKERSPALRNLPLTTDIDEVLDDPEVRIVVEVIGGEQPARDLIRRALLSGRHV
                     VTANKEVIAKHGGELRSLAAERGLAVRYEASVGGGIPIITLLERDLAANEITRIAAII
                     NGTTNYMLTRMAREHVTFEQALAQAQALGYAEPDPRNDVEGIDAAYKLAILASLAYGA
                     HVPPERVHYEGIRKLEPRDFRYAEELGYAIKLLAVADHAERGVRVRVHPALVSSDAPL
                     AKVDGVFNAIQIEGDLLGPILLQGRGAGPEPTASAVVADIVDVARTLRDECLPRTAAA
                     GANLLETEPMDTLSAQYYVRMTVADSPGVLASVAHVFGEESISIASVIQKEADEVAGT
                     AEIVIMTHHARERSLQSALRTLGSAEPVREIGNCLRVAE"
     gene            complement(3527..4711)
                     /locus_tag="VNL92_04615"
     CDS             complement(3527..4711)
                     /locus_tag="VNL92_04615"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWO94026.1"
                     /translation="MTQRRRGTRPARRSSHPVRGGRRLDAIAWIWIGAGALLVIVAVL
                     VGFGYYREYYVPPRETVLEVGDRDFKMSYFADRMRYLIADSVARGSSAPTADAPQQTL
                     ESLEHDEIIRQLGPQYGLEATDEEIDEALRRKVALLTGRLPPEGSPTPTPAGTAEPTA
                     TPVPGVGATGTPSATATPNDELFATEEEFESALEDVKDQTHLSDEELREIIHAEVLLQ
                     KLSDQLRAELPQSMEQVQMYVIAAPNRQVADQAKQRLDAGEDFLTVAEEVSPESLPPL
                     DPAAPSAPATSDESGAGTTPPPARQAPDWLIADQINMIFREAAFNSPIGTYTDVIPSD
                     RGFAILYIIGREDRPLDETQQNFLLTRALEDWFNQREGEILVRRHLDNDKSEWVLDEV
                     FG"
     gene            complement(4771..5043)
                     /gene="rpsR"
                     /locus_tag="VNL92_04620"
                     /pseudo
     CDS             complement(4771..5043)
                     /gene="rpsR"
                     /locus_tag="VNL92_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(5264..5677)
                     /locus_tag="VNL92_04625"
     CDS             complement(5264..5677)
                     /locus_tag="VNL92_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWO94027.1"
                     /translation="MASLNKVMIIGNLGTDPEMRFTANGSAVTTFRIAATRNYTSSDG
                     ERREETEWFSVVTWNKLAETCSQYLQKGRRAYVEGRLQTRSWEGQDGQRRYRTEVIAN
                     TVLFLDRAGAPGELPPVDEFGGAGLDVDAEELPFE"
     gene            complement(5763..6053)
                     /gene="rpsF"
                     /locus_tag="VNL92_04630"
     CDS             complement(5763..6053)
                     /gene="rpsF"
                     /locus_tag="VNL92_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWO94028.1"
                     /translation="MRQYELVLVLRPDLEDELIERTLERVGNAIRNTGGEVTQTDHWG
                     RRRLAYPIDRQMEGYYVVEQLRLDGQQASSVEGSVRLTEEIMRHLLVRKDEA"
     gene            6381..7217
                     /locus_tag="VNL92_04635"
     CDS             6381..7217
                     /locus_tag="VNL92_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWO94029.1"
                     /translation="MSERCETFNSGLEWLGLALALAIGLLLVVRGRRNITRSALLEPV
                     IIAVAYVTYFGVRSLTEGDVSDAFAHARDIVRLERDLGIYWEPDLQSLIVDRDCAVAL
                     ANWVYIWWHWPVIAVAAIWLLLWHPYTYRRFRNAFLISGGIGLIVFALYPVAPPRLVA
                     DLDFVDTVTMHSRGYRVLQPPAFTNQYAAMPSLHFGWNLLIGLALVRYAPLVAAKAFG
                     VVMPIAMFFAIVVTANHYILDPVAGAALALLGLALSSVLPPGRELWRRIGRRERAEEA
                     VP"
     gene            7214..7891
                     /locus_tag="VNL92_04640"
     CDS             7214..7891
                     /locus_tag="VNL92_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWO94030.1"
                     /translation="MSRPLPPQRPLAIAHRAGNDVRLLREAERLRLDIIEADVWSYRS
                     RLEVRHLKTMGRLPVLWDRWKLASGWGPRLGLVDLLEAAARESELMLDLKGTVASLPD
                     AVMEALQVRPGVGVTVCSQSWTLLKPFEALQQATVIYSIGNRRQLQELLARPPSADGF
                     SIHRRLLNAPVVASLKQIAPAVITWPVASEAEAHTLAAIGVDGVISTNLPMLGRLVAE
                     REGKRPE"
     gene            complement(7898..8308)
                     /locus_tag="VNL92_04645"
     CDS             complement(7898..8308)
                     /locus_tag="VNL92_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWO94031.1"
                     /translation="MKTGPRGVRAEAPHSPPNIMPAEARTSPDAGLLRTDPDHRTIAR
                     GGRALRLSRLEFALLQHLLESFPATMNRDELAIQLWGEASPASKGKLNLLVFRLRTKL
                     RPLAGIDIRTMRGRGYMLAISDPQEASPPRSAAG"
     gene            complement(8308..8646)
                     /locus_tag="VNL92_04650"
     CDS             complement(8308..8646)
                     /locus_tag="VNL92_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWO94032.1"
                     /translation="MKKIEAIIRPERLEAVRVALQELGCAGMTIIEVKGHGIQRGITE
                     QWRGRQYRVDFLPKLLLMVVVEDGVVEKAIDVICETAATGNIGDGKIFVTPVEEVVRV
                     RTRERGAAAM"
     gene            complement(8666..10096)
                     /locus_tag="VNL92_04655"
     CDS             complement(8666..10096)
                     /locus_tag="VNL92_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWO94033.1"
                     /translation="MSLANTLKRAFQLRRVLAVVAGIFGAMAIMSIAGAQEEDPVADL
                     TAAIDTAWLLIAAFLVFFMQAGFAMVEAGFVRAKNTTNILMKNVLDACVGGLVFWAVG
                     WGFAFGVSGDESTGFIGDGNFFLNEFEDYASWIFQFAFAATAATIVSGAMAERTKFEA
                     YLIYTACISAFVYPIVVHWAWDGNGWLSAFTEDPIGTNGYIDFAGSGVVHMVGGFAGL
                     MGAMMVGPRLGKYTDGRINAIPGHNIAIATLGMFILWFGWYGFNPGSTLALSGGFAAL
                     AAKVAVTTTLSAVAGGVTATIGSKIRTGNYDMGLTVNGILGGLVGITAPCAVVDPWAA
                     VVIGVVAAVIVMAGVEILDNLHVDDPVGAVSVHAFAGTWGVLSVGLFASEAGIAQAYV
                     PSDQYGLFLGGGIEQLGVQALGAAAIAGWTILTSGIIFGIIRATVGLRVDEEEEHRGL
                     DLGEHGVEAYHGLGSSGQTEFAGRGV"
     gene            10494..>10763
                     /locus_tag="VNL92_04660"
     CDS             10494..>10763
                     /locus_tag="VNL92_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWO94034.1"
                     /translation="MEASGDLFISLALILLTARLGGQLSSMLGLPSVFGVLITGVVLG
                     PSVTDLVEPTDSLGGLANIGVVLLLFIAGLETDLVELRTVGVPATL"
BASE COUNT         1677 a         3473 c         3514 g         2099 t
ORIGIN      
        1 ccgctcggcg tcgaactgtt gagacacaat gatgggcttc ggagccatgc tactcctatt
       61 cttgagcgac gcctgcttgc gccgcatttg attctagagc cggcccgatc agggggtcaa
      121 ggcaaactgg cggatagcgt acagcactag gcttctacca gtgcacgttc gatggcttcg
      181 cgttcggcag gcacctcgat gaggttctgg gggatggtga gggccgtctc cgggtccttg
      241 agcccgctgc cggtaatgat gcatacgatg acgtccgtgt cctcgatgcg tccttcgcgt
      301 gccaacttca tcgctccagc aaggctcgcc gcggacgcgg gctcggcaaa gatgccctcc
      361 tcgcgggaga gcagctggta cgcttcgagg atgtcgtcgt ccgtcaccgc ttcgatggca
      421 cccccggacg cgtcgcgcgc tatctccgcg cctcgccagc tcgccggatt gccgatacgt
      481 atggcggagg cgatcgtttg tgggtcgtcg acggggtgac cgagcacgat gggcgcggcg
      541 cccgctgcct gaaagcccat catgcgggga gctgtttccg tccttccggc ctccatgtat
      601 tcctcgaacc cctgccagta ggcggtgatg ttgcccgcat tgccgaccgg gatgcacagc
      661 aagtcgggcg cttcgccaag gtcatcgacg atctcgaacg ccgcggtctt ctggccagcg
      721 atgcggttcg gattgacgga gttgaccagc gcgatgttgt ggtcccccgt gaccgcgcgc
      781 gcgagctcca atgctgaatc gaagctgcca tcgatggcga gcacgcgggc gccgtaggca
      841 acggcttgcg cgagcttgcc ttgggcgatc cgtcccttgg gcacgaggac gaagcaatcc
      901 aagtcgcaga ccgcggcata ggctgcggcc gaggcgctcg tgttccccgt cgaagcacag
      961 aggacggcgc gcgctccatc ctcgatcgcc ttggcgaccg cgaccaccat gccgcggtcc
     1021 ttgaacgatc ccgttgggtt gcagctttcg agcttgaagt acagggcgcg gcagccgatg
     1081 cggtcgacga tgcgccgcga gcggatgagg ggcgtcgccc cttcgccgag cgagatcatc
     1141 ggagtggccg gagtgagggg aaggtactgg gcatagcgcg ccagaacgcc ttcactcctc
     1201 ggcaaggcgc gtcgcatact gcttgagctc ccgtatctcc cgctcgagat cgtcgatctc
     1261 gcggcgcttc tggcgctcct ccgtttgaat caaccgcagg acttgctgga cgacccgctc
     1321 gcgcacttcc tcgagctcgg tctgggcctg cgagagccgt tcctcgaaac tgttcgcccg
     1381 cgcatcgagg cgtgccgtcg cgctcgactg gccggcgagc gcttcctcca cgcgatcgat
     1441 tcgctgcgac gcggtctgtt gtgccgactc gatccggctg cgctcggctc gcaggagctc
     1501 gatggcctcg acgatctcgt cgatagcggc caagcctggc agcacggccg tatcgatgcg
     1561 caggtcgacg cggcgggcct gctccatcgc cgactgcact cgctcggtga ggacctcgag
     1621 tgacgactgc agcgaattgc tccgcgaatc gagcagggcg tggctcgcgt ccagacgatg
     1681 gatcgcctca cccaccgttc cttggcggtt ttgcgtcgtg gcgaccgtcg tcgccagctc
     1741 gtccacttcc tggcgaaggg cgctcaggcg gtcctgcacg tggcggtgag cctcatccag
     1801 tacattggtg cggctgtcga gatcgaccgt gagccgcgca acctcgtcga tgcggccctg
     1861 aagctcggcg cgggcatcgg cgttgcgttc cgaatgctcg tcctgatcgc gcatgaccgt
     1921 tgcgaggtcg cgttcggtag ccagttggcg ctggcgaata tcctccacga tcgcgcgcgc
     1981 ctcatcgagc gcacgttgga ggtgtatcgc gacgttcgtt gtgtgttctt gcctcgtgag
     2041 ccgcgtatcg attcgatcga gctggctatg caccgtgttc aggcgcgcgt tcgtctgctc
     2101 agcatcggcg cgcacgcgcc cgatctgctc gcgcaggtcg gccagctgat cctgcatcca
     2161 ttcgacggca tcgaggaatg cggcgggcag atcctccacg tcgcggtaag aggatgccga
     2221 tcggtttcgt gtcattccgc cacccgcaaa cagttgccaa tctctcgtac gggctctgcc
     2281 gagccgagcg tccggagcgc gctttgcagc gagcgttccc gcgcatggtg cgtcatgatc
     2341 acgatttcgg ccgtccctgc cacctcgtcc gcctcctttt ggatgacgga ggcgatgctg
     2401 atggattcct cgccgaagac atgagcgacg ctggcgagga caccggggct atccgcgact
     2461 gtcatgcgca cataatactg cgcactcagc gtgtccatcg gttcggtctc aagcagattg
     2521 gcgcctgctg ctgccgttcg gggcaggcat tcgtctcgca gcgtcctcgc tacatccaca
     2581 atgtccgcca cgacggcgct cgccgtaggc tccggcccgg ccccgcgtcc ttgcaggagg
     2641 atggggccaa gcaagtcgcc ctctatctgg attgcattga agacgccgtc gaccttcgcg
     2701 aggggtgcgt cactcgagac gagtgcggga tggacgcgca cgcgcacgcc gcgctcggcg
     2761 tggtcggcga cggcgagcag cttgatggca taaccgagct cttcggcgta gcgaaaatcg
     2821 cgtggttcga gcttgcggat gccttcgtag tgaactcgct ccggcggaac gtgggcaccg
     2881 taggcgagac tcgccaggat ggcgagcttg tatgccgcgt cgatgccttc gacgtcattg
     2941 cgggggtcgg gctcggcata tccgagtgct tgggcctgcg cgagcgcctg ctcgaacgta
     3001 acgtgctccc gggccatgcg ggtgagcatg tagttcgtcg ttccgttgat gatcgccgcg
     3061 atgcgggtaa tctcgttagc cgcgaggtca cgctccagga gggtgataat cggaatcccg
     3121 ccgcccacgc tggcctcgta ccgcaccgcc aagccgcgct cggccgccag tgagcgcagc
     3181 tcccccccgt gtttcgcgat gacctccttg ttagccgtca caacgtgacg cccggacagc
     3241 agcgcccggc gaataaggtc gcgggcgggt tgttcgccgc cgatgacctc gaccacgata
     3301 cggacttcgg ggtcatcgag cacctcgtcg atgtcggtcg tcagcggcag attgcgaagc
     3361 gcgggggaac gctctttcgc gaggtcgcgc acgactgccc ggcgaagggt cagcggcagg
     3421 cccgcgcgct ccgcgtagag ctgttctcgc gacgtgatcg cttcggccac ggcgccccct
     3481 acggtcccga ggccaagcag cccaaccccg atcgcgttgc gcgtcatcag ccgaagacct
     3541 cgtcgagcac ccactccgac ttgtcgttat cgaggtggcg gcgcacgagg atctcgcctt
     3601 cgcgctggtt gaaccagtcc tcgagggcac gcgtgaggag gaagttctgc tgtgtctcgt
     3661 cgaggggccg gtcctctcgc ccgatgatgt agaggatggc gaatccgcga tcgcttggga
     3721 tgacgtcggt gtaggttccg atgggcgagt tgaacgccgc ctcccggaag atcatgttga
     3781 tctgatcggc gatgagccag tcgggcgcct gtcgggccgg agggggagtg gtccccgcgc
     3841 cgctctcgtc gctggtcgct ggtgcgctcg gcgccgctgg gtccagcggg ggcaggctct
     3901 ccggcgaaac ctcctccgcc accgtgagga agtcctcgcc ggcatcgaga cgttgtttcg
     3961 cctggtcggc cacctgccgg tttggggccg cgatcacgta catctgcacc tgctccatgc
     4021 tttgaggcag ctccgcgcgc agctgatcgc taagcttctg gagcagcacc tcggcatgga
     4081 ttatctcgcg tagctcctca tcggagaggt gagtctggtc cttgacgtcc tccagcgcgc
     4141 tctcgaactc ctcttccgtc gcaaagagct cgtcgtttgg agttgcggtg gcgctgggcg
     4201 tgcctgtggc gcccacccct ggaacgggag tagccgtcgg ctcggcggtg ccggctggtg
     4261 taggtgtggg cgagccttcc ggagggagac ggcccgtcag gagcgcgacc ttgcgccgca
     4321 aggcctcgtc gatctcctcg tcggtcgctt cgagtccgta ttgcgggcca agctgccgga
     4381 tgatctcgtc gtgctcgagg ctctcgagtg tctgttgggg cgcgtctgcg gtcggggccg
     4441 agctcccccg cgcgacggag tcggcgatta ggtaacgcat gcgatctgcg aagtacgaca
     4501 ttttgaagtc gcggtcgccg acctcgagca cggtttcgcg cgggggcacg tagtactcgc
     4561 ggtagtagcc gaagccgacc aaaacggcca cgatcaccag gagcgcgccg gcgccgatcc
     4621 agatccatgc gatggcatcg agacggcgtc ctcctcgtac cgggtgcgaa gaacgacgcg
     4681 ccggtcgtgt gccgcgacgg cgttgcgtca tatcaaccta tccgcgggcc cgcggtttgt
     4741 agcggcatcc tgtgctcgtt gattcgctca gcgcggcggc ccgaatgtgg cgcccgtgat
     4801 gcggatgtgc tcgggggtgt agggcaggag ggcgatatgc cgggcgcgct tgagcgcggt
     4861 ggccaagctg cgctgatgct tcgcgcaaac tccagtcttc cggcgcggct cgatgcgtcc
     4921 gcgttcggag atgtagcgcc ggaggcggga aacatccttg tagtcgatga agctgacctt
     4981 gtcgacacag aatggacagg gcctgcgccg tggtccaccg cggccgccgc cgcgccggcg
     5041 agggccgccg cctcggcggt ccccgcgctc ggggccacgc tcgcccgcac cctcggcttg
     5101 gccagggacg ctcgtcgcgc ttgccggcgc ttcctgcacg tcggtcccga ctggctcttc
     5161 ggccggttcg gataccgctg cgtcatcgtc cgatatagtc atactggtcc tccgcgcacg
     5221 agaagtgcgg ccttaattct tcgtgaggga tgaggataca agctcattcg aagggcaact
     5281 cttcggcgtc gacatcgaga cccgcgccac cgaactcatc gacagggggc aactcgcccg
     5341 gtgcgcccgc ccggtcgagg aagaggaccg tgttagcaat gacctcggtt cggtagcggc
     5401 gttgtccgtc ctgtccttcc cacgatcgtg tctgcaagcg accctcgacg tacgctcggc
     5461 gccccttctg gaggtactgg ctgcacgttt cggcgagctt gttccaggtg acgacggaga
     5521 accactccgt ctcctcgcga cgttcaccgt cgctcgatgt gtagttgcgc gtcgcggcga
     5581 tgcggaatgt cgtcaccgcg ctgccgttcg ccgtaaagcg catctccggg tccgtaccca
     5641 ggttgccgat aatcatgacc ttgttcaggc tggccatccc gcatctcctc ctctctcgac
     5701 ttccgctccg atgcttccaa gtgcgagttg gcgattaccg gacactatca tgtcgtgccg
     5761 cgtcacgctt cgtccttgcg cacgaggaga tgccgcatga tttcctctgt tagtcggacc
     5821 gaaccctcga cggaggacgc ctgttgacca tcgagacgca actgttcgac gacgtagtag
     5881 ccttccatct ggcggtcgat gggatacgcg agcctacgtc ggccccaatg gtccgtctgt
     5941 gtgacctcgc cgccggtgtt gcgaatggcg ttgcccacac gctcgagcgt tcgctcgatg
     6001 agttcatctt cgaggtcagg gcgaaggacg aggacgagtt cgtactgacg catggtgcac
     6061 ctcctgcact gggcccgagc ctattggcgg cgggggagat ggctggatgc ggcaatgccc
     6121 gagagaacgg gcatgcttgt tgccgtccgg gacgctcgcg atcggcgcga gctcccgagt
     6181 ttgcgccatt atagcatcat cccggcgacg gctcggatgc gagcgcgcga tgttggcggc
     6241 catcgcgaag acagaagcgc gcgctgaacc gcaaagccct ttacaattgg gccgttgatg
     6301 ctgtctttac tattggctaa tactcgcgcg gcgcgctcgc gatcgccctc agtgcggtcg
     6361 taacaccgcg agacgatcgc gtgtcggagc ggtgcgagac attcaacagc ggcctggagt
     6421 ggcttggact cgcgctcgcg ttggcgatcg gacttctcct ggtcgtgcgc gggcggcgga
     6481 acatcacgcg ctccgccctg ctggagcccg tcatcatcgc ggtggcgtac gtcacgtact
     6541 tcggggtccg cagcctcacc gagggggacg tatctgacgc cttcgcgcac gcgcgggata
     6601 tcgttcggct cgagcgcgac ctcggcatct actgggagcc cgatcttcag tcgctcatcg
     6661 tcgatcggga ctgcgcagta gcgttggcga attgggtcta catctggtgg cactggccgg
     6721 ttatcgccgt ggccgccatc tggctgttgc tgtggcaccc gtacacgtac cgccggttcc
     6781 ggaatgcgtt tctcatctcc ggcggcatcg gtctcatcgt cttcgcgctc tacccggttg
     6841 cgccgccccg gctggtggcg gatctcgact ttgtggatac ggtgacgatg cactcgcgcg
     6901 gctatcgagt gctgcagcca cccgcattta cgaatcaata cgcagcgatg cccagcctcc
     6961 actttggctg gaacctgctg atagggcttg cgctcgttcg ctatgctccg ctcgttgccg
     7021 cgaaagcgtt tggcgtcgtc atgcccatcg cgatgttctt cgcgatcgtc gtgacggcga
     7081 atcactacat tctcgacccc gtggccggag cagcgctggc gctcctcggc ctagcgctgt
     7141 catcagtcct tccgcccggc agggagctat ggaggaggat agggcgccga gagcgagcgg
     7201 aggaggcggt accgtgagcc gcccattgcc accgcagcgg ccgctggcca tcgcccaccg
     7261 ggccggcaac gatgtccgcc tgctgcgcga ggccgaacgg ctgcggttgg acatcatcga
     7321 ggcggacgtg tggtcgtatc gatcacggct cgaggtgcgg catctgaaaa cgatgggccg
     7381 gctgcccgtc ctgtgggatc gctggaagct ggcgtccgga tgggggccgc gcctcgggct
     7441 ggtagacttg ctcgaggctg ccgcgcgtga atcggagctg atgctcgacc tgaaaggcac
     7501 cgtggcgagt ttgccggatg cggtgatgga ggcgttgcag gtccgtccgg gcgtcggcgt
     7561 gacggtgtgc tcccagagct ggaccctgct gaagccattc gaggctttgc agcaggcgac
     7621 ggtcatctac tcgattggca accgccgtca gttgcaggag ctcctcgcgc ggcctccttc
     7681 ggcggacggt ttctcgatcc accggcggct actgaatgcg ccggtcgtcg catccctgaa
     7741 gcagatcgca ccggcggtga tcacgtggcc agtggccagc gaagcggagg ctcacacgct
     7801 ggccgcgatt ggtgtcgatg gtgtcatcag cacgaacctc cccatgctgg gccggctcgt
     7861 cgcggagagg gaggggaagc gcccggagta ggaccagtta gcctgccgcc gagcgcggcg
     7921 gcgatgcttc ctgagggtcg ctgattgcaa gcatgtagcc ccggccgcgc atcgtgcgaa
     7981 tgtcgatgcc cgcgagcggg cgcaacttgg ttcgcaggcg aaacacgagc aggttcagct
     8041 ttcctttgct cgcggggctt gcttcccccc agagctggat tgccagctcg tcgcggttca
     8101 tcgtcgccgg aaagctttcg agcaagtgtt gtaggagggc gaactcgagg cgcgagagac
     8161 gcagcgctcg ccctccccgc gctatggtcc tgtggtccgg atcggttctg agcaggcccg
     8221 catcggggct ggttctggcc tcggccggca tgatgttggg tggggagtgg ggcgcttcgg
     8281 cccgcactcc ccgtggtccc gtcttcatca catggccgcg gcgccgcgct cgcgggtgcg
     8341 gacgcgcacg acctcttcga ccggcgtgac gaagatcttg ccgtcgccga tgttcccggt
     8401 ggcggcggtt tcgcagatca cgtcgatagc cttctcgacg acgccatcct cgacgacgac
     8461 catgagcaag agcttgggga ggaaatcgac gcggtattgc cggcctctcc actgttcggt
     8521 gatgccgcgc tggatcccgt ggcccttcac ttcgatgatg gtcatccccg cgcatcccag
     8581 ctcctgaagg gcgacgcgca ccgcttcgag tcgctcgggc cgaatgatcg cttcgatctt
     8641 tttcatgctg gctcgtcccc tctgcctaga cgccgcggcc ggcgaattcc gtctgtccgg
     8701 agcttccgag gccatggtac gcctcgacac cgtgctcgcc gaggtcgagg ccgcggtgct
     8761 cttcctcttc gtcgacacga aggccgacgg tggcgcggat gatgccgaag atgattccgg
     8821 acgtcaggat agtccagccg gcgatcgccg ccgctcccag cgcttggacg ccgagctgct
     8881 cgatgccgcc gccgaggaag aggccgtact gatcgctcgg cacgtacgcc tgcgcaatgc
     8941 ccgcctcgct tgcgaagagt cccacgctca ggacgcccca cgtaccagca aatgcgtgca
     9001 cggagaccgc accgaccggg tcgtcgacgt gcaggttatc gaggatttcc acgcccgcca
     9061 tgacgatgac cgcggcaacg acaccgatca ccaccgccgc ccatgggtcg acaacggcgc
     9121 acggagcggt gatgccgacg agccctccga ggattccgtt cacggtgagc cccatatcgt
     9181 agttgccggt ccggatcttc gagccgatgg tggcggtgac gccacccgca acggccgaca
     9241 acgttgtcgt gacggccacc ttcgccgcca acgccgcaaa gccgccactg agtgccaggg
     9301 tcgagccggg gttgaatcca taccagccga accagaggat gaacattcct aaggtcgcga
     9361 tggcgatgtt gtgacccggg atcgcgttaa ttcggccgtc ggtgtacttc ccgaggcgag
     9421 gaccaaccat catggccccc atgagcccag cgaaaccgcc aaccatgtgc acaacgcccg
     9481 agcccgcgaa atcaatgtag ccgttggtgc cgatcgggtc ttcggtgaag gcggaaagcc
     9541 agccgttgcc atcccaagcc caatggacga cgatcggata tacgaacgcc gagatgcacg
     9601 cggtgtagat gagataggcc tcgaattttg tgcgctccgc catggcgccg gacacgatgg
     9661 tcgcggctgt cgccgcgaac gcgaactgga atatccagct tgcgtaatcc tcgaactcgt
     9721 tgaggaagaa gttgccgtcg ccgatgaagc cggtggactc atcgccgctg acgccaaagg
     9781 cgaagcccca gccgacggcc caaaagacga gccctcccac gcacgcatcc agcacgttct
     9841 tcatgaggat gttggtggtg ttcttcgcgc gtacgaaccc cgcctccacc atggcgaagc
     9901 ctgcttgcat gaagaagacg aggaacgcgg cgatgaggag ccacgctgtg tcgatggccg
     9961 ccgttaggtc tgcaactgga tcttcctcct gtgccccggc gatggacatg atcgccatcg
    10021 ccccgaatat cccggccacc acggccagca ccctacgcaa ctgaaacgct ctcttgaggg
    10081 tgtttgccaa tgacaacgtg tgcctccttt cgccacgaca taccgtcccg actatcgggg
    10141 cgcagcattt cgtgctgatt tcgcgtttgt tacgcctcgg taaatcgatt gcggctccga
    10201 gtgcccggcg ggtcgcgtta ccgcagcttc atctgcccgt aacatgcttg caacatgcgc
    10261 tgcctaacct gcgaatgaac ctaaacagcc ggagcggtag caatggttgc tggacttacc
    10321 ttctttggcc tcctcgtcgg tgcccttgcg attggctcgc tttggcgccc gcgatctgag
    10381 ccttgacgcc tgtggtgatg acgctgggtc gtctcgctct ggagcgagat gaccggaatg
    10441 gtcccgatgg tgcagcgggc cacagccgcg cttcgtaggg cggccgaccg cccgtggaag
    10501 cttccggcga tctcttcatt tccctcgccc tcatcctcct caccgcccgg ttaggcggac
    10561 aactctcctc catgctgggc cttcccagcg tgttcggtgt tctcatcacc ggcgttgtcc
    10621 ttggccctag tgttacggac ttggtggagc cgacggactc tctcgggggc ctggcgaata
    10681 tcggcgtcgt gctgctgctg ttcatcgccg gcctggagac ggaccttgtg gagctccgaa
    10741 ccgtcggcgt accggcgacc ctc
//
LOCUS       DATMBB010000210         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_2283, whole genome shotgun sequence.
ACCESSION   DATMBB010000210 DATMBB010000000
VERSION     DATMBB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VNL92_04665"
     CDS             <1..247
                     /locus_tag="VNL92_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HWO94035.1"
                     /translation="RHRACYHDGMERTTISLPEDLLRRLRLLAAERRTSMATLIREAL
                     EAELASYRPTPKSLGIGDSGHSDTSPRAGEERAIPRS"
     gene            complement(350..748)
                     /locus_tag="VNL92_04670"
     CDS             complement(350..748)
                     /locus_tag="VNL92_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWO94036.1"
                     /translation="MRVIHAHEGSTSEATNQPIFEGGPVWFRGLAGRQLAPSDELSVA
                     VVQFGAGAKTRWHTHTSDQVLYVTAGIGKVGNRDGEHIISAHDCVVIPANEPHWHGAA
                     DTGSPMSHVAITLAASETKLEESDESAYGR"
     gene            complement(745..1932)
                     /locus_tag="VNL92_04675"
     CDS             complement(745..1932)
                     /locus_tag="VNL92_04675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO94037.1"
                     /translation="MSSAQRRGDDLVRLVEEMAPRILERAAQHDREGSLPFESVEALR
                     DAGYLRAAIPREYGGDGYGLPALVESQMALARADGSTALGIGMHLIVCGTEGSARSWP
                     EPARARIFEDVVRRGALLNFVAAEPELGSPQGGGRPRTSLRRDGEGRWRLSGRKTFTT
                     FAPALTYFITYVAYDDATGQVGRVAVHRELPGVRIEETWDALGMRASASHDVIFEDVP
                     LTDADILTRHSLGARSPMAESAWFPLLVGAAYFGVAEAARDYAVAFARGRTPTGYPAP
                     IAKIPHVREQVGRMDALRFAVRALMLETAEEWEAHPDARGKLGAKVAAAKRLAANSAV
                     EICEIAMRIVGGAGLRRAEPLERYFRDVRGGLINPPIEARALDTIAAAALDGGEEAGG
                     KGE"
     gene            2238..>2558
                     /locus_tag="VNL92_04680"
     CDS             2238..>2558
                     /locus_tag="VNL92_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWO94038.1"
                     /translation="MLGFEMLPGSEDEALVLTQHGEIWRVPLGDGEAERVADLSERII
                     QDPGFEEGLLGIAFSPDFATDNTLYLYFSAGEPRRTVLSRFHIAPDGTLDLGSEEVLL
                     EVAQP"
BASE COUNT          396 a          889 c          839 g          434 t
ORIGIN      
        1 gcgacatcgt gcttgctatc atgatggtat ggagcgcacc accatttccc tcccggaaga
       61 cctgctgagg cgtctccgcc tcttggcagc cgagcggcga acatccatgg cgaccctcat
      121 ccgcgaggcg ctggaggcgg agttggctag ctaccgcccg acgccgaaga gcctgggtat
      181 tggggactct ggtcacagcg acacgtctcc ccgtgcgggc gaggagcgcg ctatcccacg
      241 ttcgtagcgc cagcagatca ggcacgccag ggccgacgtt gccgtcaaga gtagtggcgc
      301 cggtgacgaa cggaggccgg ggccgaccgc cggttcgcgt gaagccaggt cagcgcccgt
      361 aggcgctctc gtccgactct tcgagcttcg tctcgctcgc ggccagggtg atggcgacgt
      421 gcgacatcgg cgagccggtg tcggctgcac cgtgccagtg cggctcgttc gccggaatca
      481 cgacacaatc gtgcgccgag atgatatgct cgccgtcccg attgccgacc ttgccgatgc
      541 ccgcggtcac gtacaggacc tgatcgctcg tgtgcgtgtg ccagcgcgtt ttggcccccg
      601 ccccaaactg cacgaccgcg acgctgagct cgtcggaggg agcgagctgc cgtcccgcga
      661 gtcctcgaaa ccacacgggc ccgccttcga agatgggctg attcgtggcc tcgctggtgg
      721 aaccttcgtg cgcatgtatc accctcattc gcccttgcct cctgcttcct cgccgccgtc
      781 gagcgctgcc gcggctatcg tatccagcgc gcgggcctcg atcggcggat tgatgagacc
      841 gccgcgtacg tcgcgaaagt agcgctcgag cggctccgct cgccgcaggc cagccccgcc
      901 gacgatgcgc atggcgatct cgcagatctc gacggcgctg tttgccgcga ggcgcttcgc
      961 cgcggccacc ttcgctccca gcttgcctcg cgcgtccgga tgggcttccc actcctcggc
     1021 ggtctcgagc atgagcgccc ggacggcgaa tcggagcgcg tccattcgcc cgacctgctc
     1081 gcggacgtgg ggaatcttcg cgatgggcgc ggggtagccg gtgggcgtgc gacctcgcgc
     1141 gaatgcgaca gcgtaatcgc gcgcggcctc cgcgacgccg aagtacgcgg cacccacgag
     1201 cagggggaac catgcggatt cggccattgg cgagcgcgca ccgagcgagt gtcgcgtcag
     1261 gatgtcggcg tccgtgagcg gtacgtcctc gaagatgacg tcgtgggacg ctgaagcgcg
     1321 cattccgagc gcatcccatg tctcttcgat ccggacgccg gggagctcgc gatgcacggc
     1381 cacgcgccct acctggccgg tcgcgtcgtc gtaggcgacg tacgtgatga agtacgtcaa
     1441 cgccggtgcg aaggtcgtga aggtcttgcg ccccgaaagt cgccatctgc cctcgccgtc
     1501 gcgccgcaga gatgtgcgtg gtctaccccc tccctgcggg gaaccgagct ccggctcggc
     1561 cgcaacgaag ttcagcagcg cgccccttcg gacgacatcc tcgaagatgc gggcgcgcgc
     1621 cggttcgggc caggagcgag cgctcccttc ggtgccgcac acgatcaagt gcatgccgat
     1681 gccgagggcg gtggacccat cggcccgggc gagtgccatc tgcgactcga cgagtgccgg
     1741 gaggccatac ccgtcgcccc cgtactcgcg ggggatggcg gcgcgcaggt atccggcatc
     1801 ccgcaaggcc tccacgcttt cgaacggcag gctcccctcg cggtcatgct gcgccgcgcg
     1861 ttccagaatg cgaggcgcca tctcctcgac gagccgcacc aagtcatcgc cgcgccgctg
     1921 cgccgaactc atcgattcgc agcgtaccac cggcgccgcg ctttggtcat gctaagctga
     1981 gagacgaagc gaatcttgca agggcttggc acatgctgcg ctggtgtctc ctcgcgctgg
     2041 ccgcactccc cttcgtctct ctccttgcct gcggcgacga tgatggcgac ggggcaagcc
     2101 catcggcgtc tcctacatcg ccgtccgcgg ctacggccac cgccacccct ggccctgggg
     2161 aagaagccat acagtccgct cccgaacccg ggtcgtatcg tctcacgccc gtggttcctg
     2221 cggcggactt cgacaacatg ctcggattcg agatgctgcc cggcagcgaa gacgaagcgc
     2281 tggtgcttac acagcacggc gagatctggc gtgtccccct gggcgatgga gaagcggaac
     2341 gcgtcgcgga cctcagcgag cggatcatcc aagacccggg ctttgaggaa gggcttctcg
     2401 gcatcgcgtt ctcacccgac ttcgcgaccg acaacacgct gtatctctac ttctcggcag
     2461 gtgagccgcg gcggaccgtc ctgtcccgct ttcatatagc cccggatggg acgcttgatc
     2521 tcggcagcga ggaagtcctg ctggaggtgg cacagcct
//
LOCUS       DATMBB010000211         9076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_354166, whole genome shotgun sequence.
ACCESSION   DATMBB010000211 DATMBB010000000
VERSION     DATMBB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354166"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VNL92_04685"
     CDS             <1..243
                     /locus_tag="VNL92_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HWO94039.1"
                     /translation="WELGGKGIPRELSDAALDGLDDDDAALAAASRRAPALDPHDEEA
                     FRRRLTGFLRRRGFSWEVTQRTIDHLWNATQAGPSA"
     gene            421..1944
                     /gene="rny"
                     /locus_tag="VNL92_04690"
     CDS             421..1944
                     /gene="rny"
                     /locus_tag="VNL92_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWO94040.1"
                     /translation="MIIGIIVGLAALVIGAAAGYAVRRSTVHEQERAARAEADRIVRE
                     AEQREKEILLEAKEEAIRARGAAETELKDRREELNRLEHRLLQKEENLERKTESLERR
                     NAALSQREAELDQRSRDINELRAQKIAEIERIAELTREEARNALLQEIDVEVRDDANR
                     RVRAADAATREEAERRARDILSTVMQRVTSEVTAETTVSVVPIPSEEMKGRIIGREGR
                     NIRALEAATGVDLIIDDTPDAVTLSGFDPIRREVARIALSKLVTDGRIHPTRIEEVVN
                     KARQEVEAGIREAGEQAALEAEVPGLHPEILRYFGRLRYRTSYGQNQLKHAVESAHIA
                     ALIAAELGADVNVARRGALLHDLGKSIDHEVEGTHAALGAELARRHGIHEAVAHCIEA
                     HHEEVEPRTVEAHIVMIADAISGSRPGARRESLERYIQRLEALENVANGFEGVDKSFA
                     IQAGREVRILVKPDEIDDLGSMRLARDISKKIEETLEYPGQIKVTVVRETRASEYAK"
     gene            2037..2813
                     /locus_tag="VNL92_04695"
     CDS             2037..2813
                     /locus_tag="VNL92_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HWO94041.1"
                     /translation="MRILMIGDVVGKPGRKAVAAIVPQLRRELRLDLVIANGENGAAG
                     RGLTPKTADELFRAGVDVITSGNHIWDNRDIYPALQEEAPILRPLNYPPGTPGRGLIR
                     CGDAVVLNAIGRTFMPMAYDDPFRLIDAALESIRPGTPIIVDFHAEATSEKQALAWHL
                     DGRVSAVLGTHTHVPTADARILPRGTAFVCDVGMVGARRSVIGDDVEAVLSRFLTAIP
                     TRLPVAEDTEADFFAVYVEIDSATGRALEIRREDRQVAIG"
     gene            2810..3685
                     /locus_tag="VNL92_04700"
     CDS             2810..3685
                     /locus_tag="VNL92_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWO94042.1"
                     /translation="MSIADFHTHSTRSDGILPPAELVRLAASRGVRLLALTDHDSMEG
                     IPEALEAARQAGLLLIPGIEMSTDIPDDEAHVLGYFLDPSDEALQAELARMREARFDR
                     GRRMVAKLNEMGVPVRWERVQEIAGDASVGRPHVARALLEGGWIASFDEAFERYIGRN
                     GPAYAERDKLTPADAVRFILDHGGVAVLAHPREIATLEPVLDQTVEAGLAGMEVYYRS
                     YSEDDIARLASVASAWGLFPLGGSDYHGQGSEGERLPGDIPLPDAAIAHFLDESARLI
                     GEERIAPYRVALASA"
     gene            3785..4702
                     /gene="argF"
                     /locus_tag="VNL92_04705"
     CDS             3785..4702
                     /gene="argF"
                     /locus_tag="VNL92_04705"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWO94043.1"
                     /translation="MRGKDLLSIADFGREELERVITTALAMKRNGGGAGLLRHRTLAL
                     LFEKPSLRTRVSFDAGMAQLGGHTIYLSQSEVGLGTREPIADVARVLDRYVDVIAART
                     FAHQTVQELAHHASVPVINALSDGEHPCQALADMLTIREKKGEVRGVRIAYIGDGNNV
                     ARSLALAATALGAEFAIASPPGYGLDEVTLAQLSAAGAGNSIRLLEDPYEAVRGADVV
                     YTDVWTSMGQESEAGSRREAFAPYRVTNELLAQAARDAIFMHDLPAHRGEEVENDVIE
                     GPHSVVFDQAENRLHAQKAVLALVLGADI"
     gene            4735..5574
                     /gene="cdaA"
                     /locus_tag="VNL92_04710"
     CDS             4735..5574
                     /gene="cdaA"
                     /locus_tag="VNL92_04710"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HWO94044.1"
                     /translation="MDLFDDVRDAFRLFEAESVLDIAIIALLIFGVLYLLRNTTAMSV
                     LRGIGLLFVVVIALARLLDLAVLSWLLDRSVTGIFIALPIIFQPELRRALERVGRTGV
                     GAWLSRNRQDQVIEELVTACLQLSERRHGALIVLERDTGLQDYVDTGIQIDAALSAAL
                     LGGLFYPNSPLHDGAVILRDGRVVAAGCTLPLSDRTVPTHVGTRHRAALGIAERTDAL
                     AIVVSEETGDTSVAWGGRMVTDVDETRFRALLYNVLGAEGAVDASGNVTSIDEERRTR
                     ATS"
     gene            5614..6855
                     /locus_tag="VNL92_04715"
     CDS             5614..6855
                     /locus_tag="VNL92_04715"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HWO94045.1"
                     /translation="MRDRRWRFAAPATRWVRENLTLGLLAVGLAVALWVYVTEEENPR
                     VTVDVTPAVRVRAVNVPEGAAVLGLGAPVAIRASAPEDVAEGLVGEDFDATVDLEGLS
                     LGSFDVPVQVNHVGDGHGVQVVQVVPGRVNVTLQPLREVELPVRAEIGLRPPIGYDIA
                     SDPEVTPDTVTVSGPEDLIERVVEVTARIDLANATADVRANVPLVASNGTSAIEGVTI
                     EPDTASVTVAIQQTRFERAFAVNPRITGNPAPGYSVASVEVEPPTVVLSGPLDALDEV
                     SAAQTNEIDLSGAISDIVQQVQLELPDEVRVRGGPDTVQVRVRIIPQTGRARFAVPPT
                     LQGVGDGLAARSLDPFVQIDVEGPLPQLQGLGPASFVAIADVSGEGEGQHEVDVSVRA
                     PAGLRVISVDPESVRVEVTRP"
     gene            6852..8234
                     /gene="der"
                     /locus_tag="VNL92_04720"
     CDS             6852..8234
                     /gene="der"
                     /locus_tag="VNL92_04720"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWO94046.1"
                     /translation="MSPAPRAPAEERPATTQLPLVVIVGRPNVGKSALFNRILGERRA
                     VVEDLPGTTRDRVGGEAEWAGRRFRVFDTGGFEEADTDHFSPLVREQIRVALEQADVI
                     LFVVDAISGLVPADEEVASLVRPTRRPVLIVANKADNQQRADRAVEFYELGLGDPYPV
                     SAIHGAGAAELLDEIVSLLPPPSETSETEAEGAEIALAIVGRPNVGKSTLLNTLVGEE
                     RAIVSEVPGTTRDIIDTVFEHGGRRLRLLDTAGIRRRGRIERGVEKHSVLRSEEAIDR
                     CDVAILLLDGPEGITAQDTHIAGYVAKAAKGLVVAVNKWDAMPPEHDPVRYTRWVSHK
                     LRFVPWADVCLISAKQGTGLETVLSMAEAAYRARASRVGTGELNRVIQAAVSAHPPGL
                     IKGKRLRIYYATQADISPPTFVFFVNDAKLLHFSYRRYLENSLRSAFGFRGTAVRLVF
                     REREALEQRD"
     gene            8256..8885
                     /gene="plsY"
                     /locus_tag="VNL92_04725"
     CDS             8256..8885
                     /gene="plsY"
                     /locus_tag="VNL92_04725"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWO94047.1"
                     /translation="MGDVLIWIAVIALSYAYGAVPWGLVIARATRGIDIRDYGSGNIG
                     FTNALRVLPLPFALAVFALDLSKGVAPILLSRLVLDDAPLQASAGVAAIVGHVWPVFL
                     RFKGGKGVATAAGGAFVLAPLPLLGLAAIALPFMFFTRYMSLTILIFAPLVSVVVLVL
                     AILGLSQWAFFAYMAAGTLIIVFRHQENIARLRAGTESRLGQRAQRSAV"
     gene            8882..>9076
                     /locus_tag="VNL92_04730"
     CDS             8882..>9076
                     /locus_tag="VNL92_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HWO94048.1"
                     /translation="MSEPYRRIVVVGATSWGTTLAILLSNLEREVSLLVRMPGEAADL
                     TLRRENHRLLPGVALPLSLTI"
BASE COUNT         1507 a         3060 c         3026 g         1483 t
ORIGIN      
        1 tgggagctcg gcgggaaggg cataccgcgt gagctcagcg acgccgccct cgatgggctc
       61 gacgacgatg acgctgccct cgcggcagcg tcgcggcggg ccccggccct cgatccccac
      121 gacgaggagg cattccgacg gcgcctcacc ggcttcctcc ggcggcgcgg cttctcgtgg
      181 gaggtcaccc agcgaaccat cgaccatttg tggaacgcga cgcaggccgg cccctccgca
      241 tagcgcgtcc ctcgcgcgag tggttctccg ccggcaactg agcgtggaac acctggcgta
      301 ctcgaacggc agtgacgcgt ttgacagccg ctgcccggca aacctatcat gagcctgaca
      361 tacgtttatt cgcgttagtt gaaatgccaa tagagtcgtt taagagcgag gttgagcgtg
      421 atgattatcg gcatcatcgt aggcctcgct gctctcgtca tcggtgctgc cgctggctac
      481 gcggtacgcc ggagtaccgt tcacgaacaa gaacgcgcgg cacgggcgga agccgatcgg
      541 atcgttcggg aagctgagca gcgagagaag gagatcctcc tcgaggcaaa ggaggaggca
      601 atccgcgccc ggggggccgc ggagacggaa ctcaaggacc gacgggagga gttgaatcgc
      661 ctcgaacatc gcctcctgca aaaggaagag aacctcgagc gtaagacgga gagcctggag
      721 cgccgcaatg cggcgctgag ccagcgcgag gcagagctcg atcagcgcag ccgcgacatc
      781 aacgagctac gtgcacaaaa gatcgccgag atcgagcgca tcgcggagct gacgcgggag
      841 gaggcgcgca acgccctcct ccaagaaatc gacgtcgagg ttcgcgatga tgcgaaccgg
      901 cgtgtgcgcg cagccgacgc cgctacgcgc gaggaggccg agcgtcgcgc ccgcgacatc
      961 ctctcgacgg tgatgcagcg cgtcacctcc gaggtcaccg ccgagacgac ggtttccgtc
     1021 gtgcccatcc cgtcggagga gatgaagggc cgcatcatcg ggcgtgaggg gcggaacatc
     1081 cgtgccctcg aggcggccac gggcgtcgac ttgatcatcg acgacacgcc cgacgccgtg
     1141 accctctccg gcttcgaccc gattcgtcgc gaagtggcgc gcatcgccct gtccaagctg
     1201 gtcacggacg gccgcatcca cccaacacgc atcgaagagg tcgtcaacaa ggcccgccaa
     1261 gaggtggagg ccggcatccg cgaggcgggc gagcaggccg cgctcgaggc cgaggtgccc
     1321 gggctgcacc cggagatcct gcgctacttc ggtagactgc gctaccgcac cagctatgga
     1381 cagaaccagc tcaagcatgc ggtcgaaagc gcccacatcg ccgccctcat cgccgccgag
     1441 ctgggcgccg atgtcaacgt ggctcgtcga ggcgccctcc tgcatgatct cggcaagtcc
     1501 atcgaccacg aagtcgaggg gacgcacgct gcgctcggcg ccgagctggc gcgcaggcat
     1561 ggcatccacg aagccgtcgc gcactgcatc gaggcacacc acgaggaggt cgaacctcgt
     1621 accgtcgaag cgcacattgt gatgatcgcc gatgcgatca gcgggagcag gcccggggct
     1681 cgccgcgagt cgctcgagcg ctacatccag cgcctcgagg cgctcgagaa cgttgccaac
     1741 ggattcgagg gcgtcgacaa gtccttcgcc atccaggcag ggcgcgaggt ccgcatcctc
     1801 gtgaagcccg acgagatcga cgatctcggg tcgatgcgcc tcgcgcgcga catcagcaag
     1861 aagatcgagg aaacgctcga atacccgggg cagatcaagg tcaccgtcgt gcgggaaact
     1921 cgcgcgtcgg agtacgcgaa gtagcccgat tccatcgccg gcgagcggca cacgggggcg
     1981 taaagaacgc ccccgttgct atatgctgag aagcgaggtt ctgtgcggag ggattcgtgc
     2041 gcattctcat gatcggcgat gtcgtgggca agccgggccg aaaggccgtc gcggccatcg
     2101 tgccccagct gcgccgcgag ctgcgcctcg atctcgtaat cgcaaatggc gagaacgggg
     2161 ccgcgggacg cggcctcacg cccaaaacgg ccgacgagct cttccgcgcc ggcgtcgacg
     2221 tcattacgtc cggcaaccac atctgggata atcgcgacat ctatccagcc ttacaggagg
     2281 aggcgccgat tctgcgtccg ctaaactatc cgcctggcac accggggcga ggccttatcc
     2341 ggtgtggcga cgccgtcgtc ctcaacgcca tcggcaggac gttcatgccc atggcctacg
     2401 atgacccgtt ccggctcatc gacgcggcgc tcgagtcgat tcggcccggc acgccgatca
     2461 tcgtcgactt ccacgccgaa gccacgagcg aaaagcaggc gctcgcctgg cacctcgatg
     2521 gtcgcgtcag cgctgtcctc ggaacgcaca cccacgtccc cacggccgat gcgcgcatcc
     2581 tgccccgagg gacggcgttc gtgtgcgacg tcgggatggt gggggcgcgc cgatccgtca
     2641 tcggcgacga cgtggaggcg gtgctgagcc gcttccttac cgcgatcccg acgcgccttc
     2701 ccgtcgccga agacacggag gccgacttct tcgccgtcta cgtcgagatc gactcggcga
     2761 cgggccgggc cttggagatt cgccgcgagg ataggcaggt ggccatcgga tgagcatcgc
     2821 cgacttccat acgcatagca cacgctcgga tggaatcctc ccgccggccg agctcgtgcg
     2881 cctcgccgcg tcgcggggcg ttcgcctcct cgccctgacg gaccacgact cgatggaggg
     2941 catccccgag gcactcgagg cggcccggca ggcggggctc ctgctcatcc ccggcatcga
     3001 aatgagcacc gacatcccag acgacgaggc ccatgtcctc ggttacttcc tcgatccctc
     3061 cgacgaggcg ctgcaggcgg agctcgcgcg catgcgagag gcgcgcttcg atcgcggccg
     3121 gcgtatggtc gcgaagctca acgagatggg cgtccccgtt cgttgggagc gcgtccagga
     3181 gattgcgggc gatgcctcgg ttggccggcc ccacgtggcg cgggcccttc ttgaaggcgg
     3241 ctggatcgcc agcttcgacg aggcgttcga acgctacatc ggacggaatg ggcccgctta
     3301 cgcggaacgg gacaagctca ccccggccga tgctgtgcga ttcatcctcg atcacggcgg
     3361 cgtcgccgtg ctcgcccacc ctcgcgagat cgcgacgctg gagccggtgc tcgatcagac
     3421 agtggaggcc ggcctcgcgg gaatggaagt gtactaccga agctacagcg aggatgacat
     3481 cgctcgtctc gcgagcgtcg cgtctgcatg ggggctgttc cccctcggag gcagcgacta
     3541 ccatggccaa ggcagcgaag gcgaacggct ccccggcgat atcccgctcc ccgatgccgc
     3601 tatcgcgcac ttcctcgatg agtcggcgcg cctcatcggc gaggagcgta tcgccccgta
     3661 tcgggtcgct ctcgcatcgg cctgagcggc ggcgcgcacg cctcgcgcgc gcccttccat
     3721 cgcgctcgtc aactcgaacg gtcatgctat actggctgcg cattttccag actgggagga
     3781 gcgggtgcgc gggaaggacc tgctctctat cgccgacttc gggcgcgagg agctggagcg
     3841 ggtcatcacc acggcactcg ccatgaagcg aaacggcgga ggcgccggcc tcctccgtca
     3901 tcgcacgctc gcgctcctgt tcgagaagcc ttcgctccgc acccgcgtta gcttcgatgc
     3961 cggcatggcg caactgggcg gccacactat ctatctgtcg cagagtgaag tcggtctcgg
     4021 cacgcgcgag cccattgccg atgtcgcccg cgtgctcgac cgctatgtcg atgtcatcgc
     4081 cgcccgaacg ttcgcgcacc agacggtgca ggagctcgcc caccacgcaa gcgtgccggt
     4141 catcaacgcg ctctccgatg gagagcatcc gtgccaggcc ttggccgaca tgctgacaat
     4201 ccgggagaag aagggcgagg tgcgcggcgt gcgcatcgcg tacatcggcg atggcaacaa
     4261 cgtcgcgcgc tccctcgcac tcgctgcaac ggcactcggc gccgagttcg ccatcgcctc
     4321 gcctccgggc tacggcttgg atgaggtcac gctggcacag ctcagcgcag cgggcgcggg
     4381 gaactccatc cgcctcctcg aagacccgta cgaggccgtc cgcggcgccg acgtcgtcta
     4441 cacggacgtg tggacgagca tgggccagga atcggaggcg gggtcgcggc gcgaggcctt
     4501 cgcgccctac cgcgtcacga acgaactctt agcgcaggcc gcacgcgatg cgatcttcat
     4561 gcacgacttg ccggcgcacc ggggagaaga ggtggagaac gacgtgatcg aagggccgca
     4621 ctccgtcgtg ttcgatcagg cggagaaccg cctccacgcc cagaaggccg tgctcgcctt
     4681 ggtgctgggc gcggacatct gaacggcccg ctgagggcag cctgaggggg acccatggac
     4741 ctcttcgatg acgtccggga tgcgtttcgc ctgttcgagg ccgagagcgt actcgacatc
     4801 gctatcatcg cgctgctcat cttcggcgtg ctgtacctgc tccgcaacac gacggccatg
     4861 tcggtcctgc gcggcatcgg cttgctgttc gtcgtcgtca tcgcgctggc gcgcctgctc
     4921 gacctcgccg tgctgtcgtg gctgctcgat cggtcggtga ccggcatctt catcgcgctt
     4981 cccatcatct tccagcccga gcttcgccgc gcgctcgagc gtgtgggacg aacaggtgtc
     5041 ggggcgtggt tatcgcgcaa ccggcaggat caggtcatcg aggagctcgt cacggcctgc
     5101 cttcagctat cggagcggcg tcacggcgcg ctcatcgtgc tcgagcggga cacaggactc
     5161 caggattacg tcgataccgg cattcaaatc gacgcggcgc tctcggccgc gctgctcggc
     5221 ggcctcttct acccgaactc cccgctgcac gatggcgcgg tcatccttcg cgatggacgc
     5281 gtcgtggccg cgggctgcac gcttcccctc tccgaccgga ccgtgccaac ccatgtgggc
     5341 acgcggcatc gcgccgccct cggcatcgcc gagcgtaccg atgcgctcgc catcgtcgtc
     5401 tcggaggaga cgggcgatac atccgtcgcc tggggcggcc gcatggtgac ggacgtcgac
     5461 gaaactcgat tccgggcgct gctctacaat gtcctcgggg ccgagggagc cgtcgacgca
     5521 tcgggcaacg tgacgtcgat cgacgaagag cggcgaacca gagccacatc ctgatacccc
     5581 cacgtgagta gattcgatcg ctggagacag aacatgcggg accgacgatg gcgattcgcc
     5641 gccccggcga ctcgctgggt gcgagagaat ctcacgcttg gcctgctggc cgtggggctc
     5701 gcggtcgcgc tgtgggtcta cgtcacggag gaggagaacc ctcgcgtcac ggtcgatgtg
     5761 acgcccgccg tccgcgtacg cgcggtcaac gttcccgagg gcgccgccgt cctcggattg
     5821 ggcgcacccg tcgcgatccg cgcatcggcg ccggaggacg tagccgaggg tctggtcggg
     5881 gaggacttcg atgccacggt cgacctcgag ggactaagcc tcggatcgtt cgatgtgccg
     5941 gtgcaagtga accatgtcgg cgatgggcac ggggtacagg tggtccaggt cgttcccggc
     6001 cgcgtgaacg tcacgctcca gccgttgcgc gaggtcgagt tgccggtgcg cgccgagatc
     6061 gggctacgcc cacccatcgg ctacgacata gcgagtgacc cagaggtgac tcccgatacc
     6121 gtgacggtgt ccggccccga ggacctgatc gagcgcgtcg tcgaggtcac cgcgcgcatc
     6181 gacctggcca acgcgacggc ggatgtgcgt gcgaacgtcc ccctggtcgc ctccaacggc
     6241 acgagcgcca tcgaaggggt caccatcgaa cctgacacgg ccagcgtcac cgttgcgatt
     6301 cagcagacgc ggttcgagcg agcgttcgcg gtcaacccac gcatcaccgg caaccccgcg
     6361 cccggatact cggtagcctc cgtcgaggtc gagcctccca cagtcgttct gagcggtcca
     6421 ctcgatgccc tcgacgaagt cagcgccgcc cagacgaacg agatcgacct gtcgggcgcg
     6481 atctcggata ttgtgcagca ggtgcagctc gaactgcccg atgaggtgcg cgtgcgcggc
     6541 gggccggaca ccgtgcaggt gcgtgtccgc atcattcccc agaccggccg agcccgcttc
     6601 gccgtgccgc ccacgctgca gggagtgggc gatggtttgg ccgcgcggag cctggacccg
     6661 ttcgtccaga tcgacgtcga gggcccgctg ccgcagcttc agggcctcgg gccggcgagc
     6721 ttcgtcgcca tcgccgacgt gtccggcgag ggcgaaggtc agcacgaggt agatgtctct
     6781 gtacgtgcgc ccgcgggcct tcgggtcatc tccgtcgatc cggagagcgt acgcgtcgag
     6841 gtcacgcgcc catgagcccc gcgcctcgcg ccccggcgga agagaggcct gcgacgacgc
     6901 agctccccct cgtggtcatc gtggggcggc cgaatgtcgg caagtccgcg ctcttcaacc
     6961 gcatcctggg ggagcgtcgc gccgttgtcg aggacttgcc ggggacgacg agggatcgcg
     7021 tcgggggcga ggccgagtgg gcggggcggc gcttccgggt cttcgataca ggcggcttcg
     7081 aggaggccga tacggatcac ttctcgcccc tcgtgcgtga acagatccgc gtggccctcg
     7141 agcaggccga cgtcatcctc ttcgtcgtcg atgccatatc gggcctggtg cccgcagacg
     7201 aggaggtcgc ctcgttggtg cggcccacgc ggcgccccgt gctgatcgtg gcgaacaagg
     7261 ccgacaatca gcagcgcgca gaccgcgccg tagagttcta cgagctcggc ctcggcgatc
     7321 cgtaccccgt ctccgccatt cacggggccg gggccgcgga gctgctcgac gagatcgtga
     7381 gcctgctccc gcccccatcc gagacaagcg aaacggaagc ggagggcgcg gagatcgccc
     7441 tcgcaatcgt cggccggccg aacgtgggga agtcgacgtt gctcaacacg ctggtgggcg
     7501 aggagcgcgc catcgtcagc gaggtccccg gcacgacgcg tgacatcatt gatacggtgt
     7561 tcgagcacgg cggacggcgc ttacgattgc tcgacacggc gggcatccgc cggcgcggcc
     7621 gcatagagcg cggtgtcgag aagcacagcg tccttcgctc ggaggaggcg atcgatcggt
     7681 gcgacgtcgc gatcctgctg ctcgatggtc cggaaggcat caccgcccag gacacgcaca
     7741 tcgcaggcta tgtggcgaag gcggcgaagg gcctcgtcgt cgcggtcaac aagtgggacg
     7801 cgatgcctcc cgagcacgac ccagtgcggt acacgcggtg ggtatcgcac aagttgcgct
     7861 tcgtgccgtg ggcggatgtg tgcctcatct ccgcaaagca aggtaccggg ctggagaccg
     7921 tgctctccat ggcggaagcc gcgtatcgtg cccgtgcgtc gcgtgtcggc acgggcgagc
     7981 tgaaccgggt gattcaggct gccgtatcgg cgcacccgcc ggggctgatc aaggggaagc
     8041 gtctccgcat ctactacgcc acgcaggcgg acatttcccc gccgaccttc gtgttcttcg
     8101 tcaacgacgc gaagttgctg cacttctcct accggcggta cctggagaac tccctgcgtt
     8161 ccgcattcgg gttccgcggc acggctgttc gcctcgtctt tcgggagcgc gaggcactcg
     8221 agcagcgcga ttagcgaagg gttggaggta cttcggtggg ggacgtgctc atatggatcg
     8281 cggtcatcgc cctcagttat gcctacggag ccgtgccgtg gggtctcgtt atcgcgaggg
     8341 cgacccgcgg catcgacatt cgcgattacg gcagtggaaa catcgggttt acgaacgcgc
     8401 tccgggtgct gccgctcccg ttcgcccttg ccgtattcgc cctcgacctt tcaaaggggg
     8461 tagcgcccat cctgctctcc cgcctcgtgc tcgatgatgc gccgctgcag gctagcgctg
     8521 gtgttgctgc catcgtgggc cacgtatggc ctgtcttcct gcgattcaaa ggcggcaagg
     8581 gcgtcgcgac tgcggccggc ggggccttcg tcctcgcgcc cctcccgctc ctcggcctgg
     8641 cggcaatcgc gctgccattc atgttcttca cacgatacat gtcgctgacc attctcatct
     8701 ttgcaccgct cgtcagcgtg gtcgtgcttg ttctcgccat ccttggtctg agccagtggg
     8761 ccttcttcgc gtacatggcc gccggcacgc tcatcatcgt ctttcgccac caggagaaca
     8821 tcgcacgctt gcgagccgga accgagtcga ggctcggcca acgcgcccag agatctgcgg
     8881 tatgagcgag ccatatcggc gcatcgtcgt tgtcggcgca accagctggg ggacgacgct
     8941 cgcgatcttg ctctcgaacc tggaacgcga ggtctccctc ctcgttcgta tgccgggcga
     9001 ggccgctgac ttgacgctgc gccgcgagaa ccaccgcctg ctccccggag tcgcgctccc
     9061 gctgagcctg accatc
//
LOCUS       DATMBB010000212         3219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_324903, whole genome shotgun sequence.
ACCESSION   DATMBB010000212 DATMBB010000000
VERSION     DATMBB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324903"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VNL92_04735"
     CDS             <1..363
                     /locus_tag="VNL92_04735"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWO94049.1"
                     /translation="GLLQSDREVQEGRERRATERASFVAWLQQRNLLETGQDDDTAEV
                     LRALLAALAATDAWAVLVGLEDLWLETQPQNTPGTSHERPNWRRKARLDLDTIASAED
                     VRAALEAVRRGRRREMPR"
     gene            360..2327
                     /gene="glgB"
                     /locus_tag="VNL92_04740"
     CDS             360..2327
                     /gene="glgB"
                     /locus_tag="VNL92_04740"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWO94050.1"
                     /translation="MTLQPDQNRAGASGVRAEADAVRHDVSLLTEDDLYLFNEGSHVR
                     LYQKLGAHPIRVGDVDGTYFAVWAPDAEQVGVMGDFNGWSPESHLLRPRGQSGIWEGF
                     VPGIGAGAVYKYRIHSRFHGYRVDKADPFAFRAELPPKTASIVDRCEYEWGDGQWLRE
                     RAGRNSMTAPISIYEMHLGSWARPPDDGRRWLSYREIAPKLADYVKQLGFTHVELLPV
                     MEHPFYGSWGYQTTGYFAPTSRYGTPEDFAFFVDHLHQQGVGVILDWVPSHFPADEHG
                     LAFFDGTHLYEHADPREGFHPDWNSYIPNYGRNEVRSFFLSSALFWLDRYHIDGLRVD
                     AVASMLYRDYSRREGEWIPNIYGGRENLEAISFLRRFNEEVYQHYPDVQTTAEESTAW
                     PMVSRPTYVGGLGFGLKWDMGWMHDTLHYMQRDPIYRRFHHGEITFRMIYAFNENFVL
                     PLSHDEVVHGKGSLLGMMPGDDWQKFANLRLLLAYMAGQPGKKLLFMGGELGQWSEWN
                     HEASLDWDLTQWDQHAGIQRLVGHLNRTYQGERAMHELDTHPDGFLWIDANDSESSTL
                     SWIRRARSTDEDIVLLFNFTPVPRENYRVGVPRAGVWREIVNTDAVEYGGSGIGNLGE
                     VQTAPIPLHGQPYSLTLTLPPLAALFLKRAE"
     gene            2333..>3219
                     /locus_tag="VNL92_04745"
     CDS             2333..>3219
                     /locus_tag="VNL92_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWO94051.1"
                     /translation="MRDWRPRHGALYLGQGTTRFRVWAPGHDVELHLLTPTDRYIPMK
                     PDGCGYHEEAVAGTSPGDLYRYRLDGGEEWPDPASRSQPEGPHGPSQIVDPEFAWTDE
                     AWAGVPLERYVVYEAHVGAFTGEGTFRAMIPELPYLRELGITVLELMPVAQFPGERNW
                     GYDGVFPYAAQTSYGGIDSLRSLVDACHAHGLAVCLDVVHNHLGPEGNYFSNFGPYFT
                     DRYRTPWGAAINYDGPESDEVRAFFIENALYWTLDCHIDALRLDAVHAIADNSPVRYL
                     EDLAATVHRRTAHLSRPTYV"
BASE COUNT          597 a         1060 c         1015 g          547 t
ORIGIN      
        1 ggcctgcttc aaagcgatcg cgaagtacag gaggggcgcg aacggagggc gaccgagcgg
       61 gcctccttcg tcgcgtggct gcagcagcgc aacctgctgg agacgggcca ggacgacgac
      121 acggcagaag tgctgcgggc cctgctcgcg gcactcgcgg caacggacgc gtgggccgtc
      181 ctcgtcgggc tcgaagacct atggctcgaa acccagcctc agaacacgcc gggcacaagc
      241 cacgagcgtc cgaactggcg gcgcaaggct cgcctcgatc tcgatacgat agcttcagcc
      301 gaggacgtgc gcgccgcgct cgaagcggtg cggcgaggcc ggagaaggga gatgcccaga
      361 tgacgctgca accggaccaa aatcgcgcag gagcatcggg cgtgcgggcc gaagctgatg
      421 ccgtgcgcca cgacgtatcg cttctcaccg aggacgacct gtacctcttc aacgaaggct
      481 cgcatgttcg cctctatcag aagctcggtg cccaccccat tcgcgtcggt gatgtggacg
      541 gaacgtactt cgcggtctgg gccccggacg cggaacaggt cggcgtcatg ggggacttca
      601 acggctggag tcccgagtcg cacctgctcc gtcctcgggg acagtcaggc atctgggaag
      661 ggttcgtgcc cggcatcgga gcaggcgccg tctataagta tcgaatccac tcccgcttcc
      721 acggctatcg agtcgacaag gcggatccct tcgccttccg ggcggagctt cccccgaaaa
      781 ccgcgtccat cgtcgatcgc tgcgagtacg aatggggcga cgggcagtgg ctgcgcgagc
      841 gggccggccg taactcgatg acggccccga tatcgatcta cgagatgcac ctcggctcct
      901 gggctcgccc tccggacgac gggcgccgct ggttgtccta tcgggagatc gcgccgaagc
      961 tcgccgacta cgtgaagcaa ctcggcttca cgcatgtcga actgcttccc gtgatggagc
     1021 atcccttcta cggttcctgg ggctaccaga cgaccggata tttcgcgccg acgagccggt
     1081 acgggactcc cgaggacttc gccttcttcg tcgaccatct ccaccagcag ggagttggcg
     1141 ttattctcga ttgggtcccg tcgcacttcc cagccgatga gcacggtctc gccttcttcg
     1201 acgggacgca tctctacgag cacgcggacc cgcgtgaagg cttccacccc gattggaaca
     1261 gctacatccc gaactatgga cgaaacgagg tgcgcagctt cttcctgagc agcgccctgt
     1321 tctggctcga ccgctatcac atcgatggtc tccgcgtcga cgccgtcgcc tcgatgctct
     1381 accgcgatta ctctcgccga gagggagagt ggatccccaa catctacggg ggacgggaga
     1441 acctggaggc gatcagcttc ctgcgtcggt tcaacgagga agtgtaccaa cactaccccg
     1501 atgtccagac cacggcagag gagtcgacgg cgtggccgat ggtgtcgcga ccgacgtatg
     1561 taggcggcct tggcttcggc ctgaagtggg atatgggctg gatgcatgac acgctgcact
     1621 acatgcagcg cgaccccatc taccgccgct tccaccacgg agagatcacc ttcaggatga
     1681 tctacgcatt caacgagaac ttcgtgctgc ccctttccca cgacgaagtc gtgcacggca
     1741 aaggttcgct cctcggcatg atgccgggtg acgattggca aaagttcgcc aacctgcgtc
     1801 tcctgcttgc gtacatggct ggtcagccgg ggaagaagct gctcttcatg ggcggcgagc
     1861 tcggccaatg gagcgagtgg aaccacgaag cgagcctgga ttgggacctg acgcagtggg
     1921 accagcacgc cggcatccag cgccttgtcg gtcacctgaa ccgcacgtat cagggcgagc
     1981 gcgccatgca cgagctcgat acgcaccccg atggctttct atggatcgat gccaacgaca
     2041 gcgagtcgag cacgctgagc tggatacggc gggcacgctc caccgacgaa gacatcgtgt
     2101 tgctgttcaa cttcacaccg gtgccgagag agaactaccg cgtcggcgtg ccgagggcgg
     2161 gtgtgtggag ggagatcgtg aacacggacg ccgtggagta tgggggatcg ggcatcggga
     2221 atctcggcga agtacagacg gcgcccatcc ctctccacgg acagccgtat tcgctcacgc
     2281 tcacactgcc acccctcgcg gcgctctttc tgaagcgtgc cgagtgagcc aagtgcgcga
     2341 ctggcggcca cggcacggtg cgctgtatct aggccagggc acaacacgat ttcgcgtgtg
     2401 ggcgccgggc catgacgtcg agttgcactt gctcacgccc acggatcgct acatcccgat
     2461 gaagccagac ggctgcggct accatgagga ggcggtagcc ggcaccagcc ccggtgacct
     2521 ctaccgctat cgactcgacg gcggcgagga gtggcctgac ccggcgtcac gctcccagcc
     2581 cgaaggtccg cacggcccct cgcagattgt cgaccccgag ttcgcgtgga cggacgaggc
     2641 ctgggcgggc gtccccctcg agcgatacgt ggtgtacgaa gcgcatgtcg gcgcgttcac
     2701 gggcgagggg accttccgcg cgatgattcc cgaactgcca tacctgcggg agctcggcat
     2761 caccgtcttg gagctcatgc ctgtcgccca gtttccggga gaacgcaact gggggtacga
     2821 cggcgtcttt ccctatgcgg cgcagaccag ctacggcgga atcgacagcc tgcgcagcct
     2881 cgtcgacgcc tgccacgccc acggcctcgc cgtttgcctc gatgtcgtcc acaaccacct
     2941 cgggccggag ggcaactact tctcgaactt cggcccgtac ttcactgacc gctaccggac
     3001 gccctggggc gctgccatca actacgatgg accggaaagc gacgaagtgc gggcattctt
     3061 catcgagaac gcgctctact ggacgctcga ttgccacatc gatgcgctac gcctcgatgc
     3121 cgtgcatgcc atcgcagaca actcgcccgt gcgctacctc gaggacctcg cagcgacggt
     3181 ccaccgccga accgcgcacc tgtcgcggcc cacgtacgt
//
LOCUS       DATMBB010000213         5538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_156890, whole genome shotgun sequence.
ACCESSION   DATMBB010000213 DATMBB010000000
VERSION     DATMBB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156890"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="clpX"
                     /locus_tag="VNL92_04750"
     CDS             <1..943
                     /gene="clpX"
                     /locus_tag="VNL92_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007952676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWO94052.1"
                     /translation="VGPTGCGKTLLARTLAKALDVPFTIADATALTEAGYVGEDVENI
                     LLHLIQAADFDIARAERGIVYIDEIDKIARKADNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPHQDFIQINTQNILFICGGAFEGLDKIISRRLGHDRRALGFRPVSLD
                     SKETDHILAEVNHDDLLQYGIIPEFAGRVPMVVALEELDRAAMVRILTEPKNALVRQF
                     QRFFSMDNVELSFTQDALIAIAEEAMRHKTGARGLRTVLEDVLLDVMYEIPSRGDVTK
                     CVVSAETILSRKRALLLTQSGQSVEFDESLALRDESA"
     gene            1322..2188
                     /locus_tag="VNL92_04755"
     CDS             1322..2188
                     /locus_tag="VNL92_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO94053.1"
                     /translation="MRYALNLPTGGPCGDPATLADFAELAERSGWDAVFVEDYIVYQG
                     REDLPTHDPWIALAAMATRTSRIRLGTMVTPLARRRPWKVAREATSLDHLSNGRVTLS
                     VGLGDASTDSSFWKFKEELDDRRRAAMLDEALDIVAGLWTGEPFSYDGAHFKVDRVTF
                     RPRPIQTPRIPIWVGGAYPNEGPMRRAARWDGANFYRAKTPGSAGDSGRGLTLDEFIR
                     LREYILSRRKTDAPLDLVVHPQRHPRSAESEVSRLRAFAEAGASWSQVWIPPGERHQM
                     ESEIAVGPLLIV"
     gene            complement(2458..3288)
                     /locus_tag="VNL92_04760"
     CDS             complement(2458..3288)
                     /locus_tag="VNL92_04760"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HWO94054.1"
                     /translation="MLGAIALPQRDRAAILSVLAALIALSWLTLADPLGLGIGAALDH
                     EEIFEGKDAVGILARLPLFVAAWVLMTVAMMLASTLPTQQAVAAIAARRGPMGPGVSA
                     LLFTAGYVAVWSAFGVAAFGVDLGVHRLVEVVHVLDDHELLLPGGLTALAGLYQFTEL
                     KDRCLTVCRTPMSFVMTRWRGGRHGALAMGLDHGLYCLGCCWPLMLLMFAFAATSLPL
                     MLLAAVYFFAEKALVQGDSVSRAVGVAFVAVGLGLLAIEVGAIVLDAGAVCAHGEGAC
                     "
     gene            complement(3296..3922)
                     /locus_tag="VNL92_04765"
     CDS             complement(3296..3922)
                     /locus_tag="VNL92_04765"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HWO94055.1"
                     /translation="MTTQAGVKVKWRMKGKYLETCSCDFGCPCNFEAPPTQGYCSYVL
                     GWHIDEGQIGDVRVDGLNIAAAGWFPEAMHLGNGTMIPYVDERATPEQRQALLTMISG
                     QLGGLPWEILAAITPNVREPRFVPIQFKVDGRNSHLKIGDVAEAGLTPIKNPVTGEPE
                     NPRVVLPEGFIFHEGHVAQSTASWVKDGDIQRDQTGQNGFYAEVDYSN"
     gene            complement(4106..4402)
                     /locus_tag="VNL92_04770"
     CDS             complement(4106..4402)
                     /locus_tag="VNL92_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWO94056.1"
                     /translation="MYGTVARMKAKPGMGQQLLDVARSQWEERIPGIVSECIYQADAD
                     GDEYWLTVVFESKEAYRKNAESPEQDARYRKLRALMAADPEWHDGEVVLTAPPK"
     gene            complement(4536..5219)
                     /locus_tag="VNL92_04775"
     CDS             complement(4536..5219)
                     /locus_tag="VNL92_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HWO94057.1"
                     /translation="MTTRAYGLWDTAMVQTAPPRPLLRGILHLVAAMFAIVGLVALLL
                     TATSPSGYVGASIYGAGLILLYSTSAAYHRLSRRPALHGVLRRLDHGMIFVLIGATYT
                     PFCLGVLDLAWGIPMLAVVWSIAALGVMLKVAWIGLPKWLGVSLYVALGWMAVVIAPE
                     VASELAIGGLALLVGGGVLYTVGGVAYGLAWPNPWPRVFGFHEVFHSFVIGGSALHYA
                     AVALYAVGP"
     gene            complement(5273..>5538)
                     /locus_tag="VNL92_04780"
     CDS             complement(5273..>5538)
                     /locus_tag="VNL92_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006376851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWO94058.1"
                     /translation="WLRERGVAVLGCDGVSDVIPSGIEGGAGLPIHFGAIVAMGVHLI
                     DNAQLEDLGAACERAGRWEFLLTLAPLRLLKGTASPVNPIAML"
BASE COUNT         1066 a         1881 c         1693 g          898 t
ORIGIN      
        1 cgtcggaccg accggctgcg gcaagacgct tctcgcccgc accctggcca aggcgctcga
       61 tgtcccgttc accatcgccg atgcgacggc gctgaccgag gcgggctacg tgggcgagga
      121 tgtcgagaac atcctgctcc acctcattca ggccgccgac ttcgacatcg cccgcgcaga
      181 acgcggcatc gtctatatcg atgagatcga caagatcgcc cggaaggcgg acaacccctc
      241 gatcacgcgc gacgtctcgg gcgaaggcgt ccagcaggcg ctgctgaaga tcatggaggg
      301 aaccgtcgcg agcgtgccgc cgcaaggcgg acgaaagcat ccgcatcagg acttcatcca
      361 gatcaacacg cagaacatcc tgtttatctg tggcggggcc ttcgaggggc tcgacaaaat
      421 catcagccgc cgcctcggac acgaccgaag ggccctcggc ttccgtcccg tctccctcga
      481 tagcaaggag acggatcaca tcctcgccga ggtcaaccac gacgaccttt tgcaatacgg
      541 catcatcccc gagttcgctg gtcgcgtgcc gatggtcgtt gctctcgagg agttggaccg
      601 tgccgccatg gtccgcatcc tcaccgagcc gaagaacgcg ctggtccgtc agttccagcg
      661 attcttcagc atggacaacg tcgagctcag cttcacgcag gacgccctca tcgccatcgc
      721 cgaagaggcg atgcgtcaca agacgggcgc gcgcggcctc cgcacggtgc tcgaagatgt
      781 gctgctcgac gtgatgtacg agataccatc gcgcggcgac gtcacgaagt gcgtcgtgag
      841 cgcggagacc atcctatcgc gcaagcgcgc ccttttgctc acgcaatcgg gccagtcggt
      901 cgagttcgac gagagcctcg cgctgcggga cgaatcggcc taggcccatc tgcacgtgaa
      961 cgcgaaaagg ccgcccacct gggcggcctt ttccatctgc caggccgcgg cctagcgtgt
     1021 cacctccgaa gagatgtccc gtccgagacc gcgaccgcag atacgcttat cgtccatggg
     1081 catcacctcc ttcactgcgc gacatcgtag catggcggcc tcgcgacggc aatgctcgcc
     1141 tggcacccgc cgccctgacg actgccctag caggcgctct caccgtgcac acggcgtcgg
     1201 tatcgagcgc gatggcgccg agcagcaaac gccggccgag ggctgagacg acgcttccac
     1261 aggcgtcgct tccgtggtta agctgtcgta gttgcgcacg cgcaccagga gggctggcaa
     1321 catgcgctac gcgctaaacc tcccgacggg tggcccttgt ggagatccgg ccacccttgc
     1381 cgacttcgct gagcttgccg agcgatcggg ttgggatgca gtcttcgttg aggactacat
     1441 cgtctatcaa ggccgcgaag acctgcccac ccacgatccg tggatcgcat tggcggcgat
     1501 ggctacgcgc accagtcgaa ttcgtttggg cacgatggtt acgccgctgg ctcggcggcg
     1561 gccgtggaag gttgcgaggg aggccaccag cctcgaccac ctctcgaacg gacgagtaac
     1621 cctctccgtc ggactgggcg atgcttcaac cgactcaagt ttctggaagt tcaaagagga
     1681 actggatgac cgccgccgag cagctatgct cgatgaagcg ctggacatcg tggcgggcct
     1741 ctggacgggc gagccgttca gctacgacgg agcgcacttc aaggtcgatc gggtgacgtt
     1801 tcgtccccgt ccaatacaga ctccgcgcat tccgatttgg gtcggcggag catacccgaa
     1861 tgagggcccg atgcgccgcg cagcacgctg ggatggcgcg aacttctacc gagccaagac
     1921 gcccgggagc gccggggaca gcggccgggg cctcactttg gacgagttca tccgactgcg
     1981 tgagtacatc ctcagccggc ggaagaccga cgcacctctt gatctggtgg tccatccgca
     2041 acgtcatcct cgaagcgcgg agagtgaggt ctccagactg agggcattcg ctgaggctgg
     2101 agcgagttgg agccaagtgt ggattcctcc gggcgagaga catcagatgg agagcgagat
     2161 tgctgtcggt ccgctcctca ttgtgtgact gcgtgggttc aggccgctgc gaggagttgc
     2221 gcgtcgcgct gccgatccgt gaggctctcg aaagtctcct gaccctggtc ctgcccgctg
     2281 agcggcgagc ttcgggcgct gcaaacgatg gacacgggcc gtcctccctt gctcggtgct
     2341 gattcccgca caccaaggct agaacgagac ggccctcaat atttggtaat tggtgccaat
     2401 ctccctacca tggaaaaagg caatgctcgc ccgccacccg ccgccctgac gactgcccta
     2461 gcaggcgccc tcaccgtgtg cgcacacggc gccggcatcg agcacgatgg cgccgacctc
     2521 gatggcaagt aacccaagtc cgacggcgac gaacgccacg ccgaccgccc tgctcacgct
     2581 atcgccctga acaagcgcct tctccgcgaa gaagtacact gccgcaagga gcatgagcgg
     2641 aagactcgtc gcggcaaacg cgaacatcag gagcatcagg ggccagcagc agcccaggca
     2701 gtagagcccg tggtctaacc ccatcgcaag cgcgccatgc ctgccgccgc gccagcgtgt
     2761 catcacgaac gacatgggtg tgcggcagac ggtgaggcag cgatccttga gctcggtgaa
     2821 ctggtagaga ccagccaggg ccgtcaatcc tcccgggagg aggagctcgt ggtcatcgag
     2881 gacgtggacg acctcaacca gcctgtgtac gccgagatcg acgccgaacg ctgcgacacc
     2941 gaaggcgctc caaactgcca cgtagccggc agtaaagagc aatgctgaga ctccggggcc
     3001 catggggccg cggcgagcag cgatcgcagc aaccgcctgc tgcgtcggca gcgtcgatgc
     3061 caacatcatc gcgaccgtca tcagcaccca ggccgccacg aacagcggaa ggcgcgcgag
     3121 gatgccgacg gcgtccttgc cctcgaaaat ctcctcgtgg tccagcgccg ccccgatgcc
     3181 gaggccgagc ggatcggcga gcgtcagcca cgagagcgcg atgagggcag ccagaaccga
     3241 taggatcgcc gcacgatccc gctgcgggag tgcgatggcg cccagcacgc cggtactagt
     3301 tgctgtaatc gacttcggcg tagaacccgt tctgcccggt ctggtctcgt tggatgtcgc
     3361 catccttcac ccagctcgcc gtcgactgcg caacatgtcc ctcgtggaag atgaaaccct
     3421 ccggcagcac gacacgcggg ttctcaggct cgcccgtcac gggattcttg attggcgtca
     3481 gaccggcctc cgcaacgtcg ccgatcttca ggtgcgagtt tcgcccgtcg actttgaact
     3541 ggatgggcac gaaccgcggc tccctcacat tcggcgtaat ggcggcaagg atctcccagg
     3601 gtagcccgcc cagctgaccc gatatcatgg tcagcaacgc ttggcgctgc tcgggagtgg
     3661 cgcgctcatc cacatatgga atcatcgtgc cgttgccgag atgcatcgcc tcggggaacc
     3721 agcccgcggc cgcgatattc agcccatcga ctcgcacgtc gccgatctgg ccctcatcga
     3781 tgtgccatcc caatacatag ctgcagtatc cctgcgtagg aggagcctcg aagttgcagg
     3841 ggcaaccgaa gtcgcaactg cacgtctcga ggtacttccc cttcatgcgc cacttcacct
     3901 ttacgccggc ttgtgttgtc atggtgctgc accctcttca ccctcgcgcc cacgcggacc
     3961 tatccgattc gttgggaggc cgcgctccgc caaccgatcg gagcccactt ccccaactct
     4021 tcctcatcac agcggatgac gaagtggcgc aattcttcag caaacgcgga cacttgtcaa
     4081 gaattggtgc gcctcccccg aaagattact tgggtggcgc ggtgagaacc acttcgccgt
     4141 cgtgccactc agggtcggcg gccatgagcg cgcgcaactt ccggtatcga gcatcctgct
     4201 ccggcgattc cgcgttcttg cggtacgcct ccttgctctc gaacaccacg gtgagccagt
     4261 actcgtcgcc gtccgcgtcg gcctggtaga tgcactccga aacgatgccc ggaatgcgct
     4321 cctcccactg cgatcgcgct acatcgagca gttgctgccc catccctggc ttcgccttca
     4381 tccgagccac cgttccgtac atcgcccctt cctccttacg taacatactg ccgaagttcg
     4441 ggctgagcgc ccgaccccta acttcggccg aagtgcgcag aattttcggg cctagccacg
     4501 ccgatgtcaa ttcgaagagg agagacctgg acgagctacg ggccgacggc gtacagggcg
     4561 accgccgcat agtgcagggc actgccaccg atgacgaagc tgtgaaacac ttcgtggaag
     4621 ccgaacacgc gcggccatgg gttcggccac gccagcccat acgcgacgcc gccgaccgtg
     4681 tacaacacgc cgcccccgac cagcaacgcc agcccgccta tcgcgagctc ggacgcgacc
     4741 tccggggcga tgacaaccgc catccacccc agcgcgacgt acagcgacac gccgagccac
     4801 ttcggaagcc caatccatgc gaccttcagc atcacgccga gggccgcgat gctccaaacg
     4861 acggcgagca tcgggatgcc ccacgccagg tcgagcacgc cgagacagaa cggcgtgtag
     4921 gtggcgccga tgagcacgaa gatcatcccg tggtcgagcc gccgcagcac cccgtggagc
     4981 gcaggccggc gagacagccg gtggtaggca gcgctcgtgc tgtacagcag gatgaggccg
     5041 gcgccgtaga tagacgcccc cacgtacccg ctcggcgacg ttgcagtcag gaggagggcc
     5101 acgaggccca caatcgcgaa catcgccgcg acgaggtgga ggatgccgcg cagcagcgga
     5161 cgcggcggcg cagtctgaac catcgcggtg tcccagaggc catacgcacg cgtcgtcaca
     5221 agctcattat gccctatgcc cggctaaaga caatcgggga agtgcaccaa acctatagca
     5281 ttgctatcgg gttgacgggc gaggcggtcc ccttcagcag acgcagcggc gcgagggtca
     5341 gcaggaactc ccagcgcccc gccctctcgc acgcggcccc caggtcctcg agctgcgcgt
     5401 tgtcgatgag gtggaccccc atggcgacga tcgcgccgaa gtggatcggc aagcccgcgc
     5461 ccccctcgat gccggagggg atgacgtcgc tcacgccgtc gcagccaaga acggcgacgc
     5521 cgcgctcgcg caaccaag
//
LOCUS       DATMBB010000214         2302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_407801, whole genome shotgun sequence.
ACCESSION   DATMBB010000214 DATMBB010000000
VERSION     DATMBB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407801"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1089)
                     /locus_tag="VNL92_04785"
     CDS             complement(16..1089)
                     /locus_tag="VNL92_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWO94059.1"
                     /translation="MRFRLNPIPSLLGLFSHDIGIDLGTANTLVMVRGRGIVINEPSV
                     VAIDRSTKKLLAIGAEAKRMVGRTPANIIAVRPLKDGVISDFEITEKMLHYFIRQVHE
                     RFGAGGLPRPRVVVGIPSGVTEVEKRAVHDATLSAGARVAYLIEEPMAAAIGAGLPVM
                     EAGGCMMVDIGGGTTEVAVISLGGVVVSTSIRVAGDEMDQDLIAYARQVHNMLIGERT
                     AEQIKIEAGTAYPLEEEIMVTLRGRDLATGLPKSVEVSSVEIRDALSGSINTIVDAVR
                     STIEITPPELIADLMTQGIALAGGGAMLQGLDRRLAQDTRFPVYVAEDPFTCVVRGAG
                     EVLEEAEILRRVQSNLATRKPSR"
     gene            complement(1205..1984)
                     /locus_tag="VNL92_04790"
     CDS             complement(1205..1984)
                     /locus_tag="VNL92_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO94060.1"
                     /translation="MTYQDLLLEREDGIATITLNKPDKLNAMSWTTWRELRESVAECD
                     ADDDVKVIIVTGAGRGFSSGTDLTAASQDEIPSRRETMRSGYLSAVSLIHSAKPTIAA
                     VNGIAAGAGLSIALACDIRIGAASSRYSAIFVKRALSADFGCSYLLPRVVGMSNAMRL
                     MYTGDIIDAQEALRIGLLSEVVPDEALIQRARELSLPIAHGPSIAIEATKRLVYRQIE
                     AEIDAHVEYEEYLQRLTHQSEDALEGRMSFLEKREPRFHGR"
     gene            complement(1981..>2302)
                     /locus_tag="VNL92_04795"
     CDS             complement(1981..>2302)
                     /locus_tag="VNL92_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HWO94061.1"
                     /translation="LRRAGVRMAGAEPPRREGPLAGKTFVLTGSLERFPRSRATQIIE
                     SLGGAVASSVTKKTDYVVVGEAPGSKLERAREIGVAVLDEDAFVDLLRDCGATEAAAG
                     RGSS"
BASE COUNT          397 a          773 c          697 g          435 t
ORIGIN      
        1 catgcccgcg tcattctatc gtgaaggctt tctcgttgcc aagttcgatt ggacgcgccg
       61 cagaatttcc gcttcctcga gcacttcgcc cgctccgcgc accacgcacg tgaagggatc
      121 ctccgcgacg taaacgggga aacgcgtgtc ctgcgccaag cgccggtcga ggccctgaag
      181 catggctccc ccgccggcca gcgcaatacc ctgcgtcatc aggtcggcaa tgagctccgg
      241 cggcgtaatc tctattgtgc tccgaaccgc atcgacgatc gtgttgatgg agccggacag
      301 tgcgtcgcgt atttcgacgc tgctcacctc caccgacttc ggcaggcccg tcgcgaggtc
      361 gcggccacgg agcgtgacca tgatctcctc ttcgagcggg tacgccgtgc cggcctcgat
      421 cttgatctgt tcggccgtgc gttcaccgat gagcatgttg tggacttggc gggcgtaggc
      481 gatgaggtcc tgatccatct cgtcgcccgc aacgcgaatc gacgtgctca cgacgacacc
      541 gcccagcgag atgacggcga cttctgtcgt gccgccgccg atgtccacca tcatacagcc
      601 gcctgcctcc atcacgggca aaccggcccc gatcgccgcc gccatcggct cctctatcag
      661 ataggcgaca cgggctccgg cgctcagcgt cgcgtcatgc acggcacgct tctcgacttc
      721 cgtgacgccg ctcggaatgc caacgacgac ccttggccgc ggcagaccgc ccgcgccgaa
      781 gcgctcgtgc acctggcgaa tgaagtaatg cagcatcttc tccgtgattt cgaagtcgga
      841 gatgacgccg tccttgagag ggcgcacagc gatgatgttg gcaggtgtgc gtcccaccat
      901 tcgcttcgcc tcggccccga tcgcgagcag cttcttggta gagcggtcga tggcgacaac
      961 cgagggttcg ttgatgacga tgcccctgcc tcgcaccatg acgagcgtgt tcgccgtgcc
     1021 gagatcgatg ccgatatcgt gcgagaagag gccgagcagc gatgggatcg ggttcagcct
     1081 gaagcgcatg gcctccactc gaaagtcgat tattcaggaa gaccgcttgt cggcagtata
     1141 ccctggaaca ggcttagcga ccccgttttg tgcttcagga acgctggaag gttatcggaa
     1201 acgtctaacg tccgtggaag cgcggctctc gcttttcgag gaagctcatt cgcccctcga
     1261 gtgcgtcctc actctgatgc gtgagccgct gcaggtactc ctcgtattcg acgtgggcat
     1321 ctatttcggc ctcgatctgg cgatagacga gccgctttgt cgcttcgata gctatggacg
     1381 gcccgtgcgc gatgggcagg ctcaactctc gagcccgctg tatcagcgcc tcatcgggca
     1441 ccacttcgga gagcaggccg atccgcaatg cctcctgtgc atcaatgatg tcgcccgtgt
     1501 acatgagccg catcgcgttc gacatcccga ctacacgcgg tagtaggtag ctgcagccga
     1561 agtccgccga gagagcgcgc ttgacgaaga tggccgagta ccgagacgag gccgcgccga
     1621 tacgaatgtc gcaggcgagc gcgatcgaga ggccggcgcc cgctgcaatg ccgttgacgg
     1681 cggcgatggt gggctttgcg gaatggatca ggctcaccgc cgagaggtac ccgcttcgca
     1741 tcgtctcacg gcgactcggg atctcgtcct gggaggcagc cgtcaggtcg gtgcccgaag
     1801 agaagccgcg ccctgccccc gtcacgatga tcaccttgac atcgtcgtcc gcgtcacact
     1861 ccgcgacgga ctcgcgcagc tctcgccagg tcgtccacga catggcgttg agcttgtcgg
     1921 gcttgttcag cgtgatggtt gctatgccat cttcccgttc gagcagaagg tcctgatacg
     1981 tcacgagctc cccctcccag ccgcggcctc ggtcgcgccg caatcgcgca gcagatctac
     2041 gaaagcgtcc tcatccagca cagcgacacc gatttctcgc gcccgctcga gcttcgagcc
     2101 cggcgcctcg cccacgacaa cgtagtcggt cttcttcgtc acgctcgacg cgacggcgcc
     2161 gcccaggctc tcgatgatct gcgtcgcccg cgaccggggg aagcgctcca gcgaaccggt
     2221 gagcacgaag gtcttccctg ccaacggccc ttcgcgacgg gggggctctg ccccggccat
     2281 gcgaacgccg gctctacgca gc
//
LOCUS       DATMBB010000215         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_178105, whole genome shotgun sequence.
ACCESSION   DATMBB010000215 DATMBB010000000
VERSION     DATMBB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178105"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VNL92_04800"
     CDS             <1..949
                     /locus_tag="VNL92_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWO94062.1"
                     /translation="QAVLNSCRYLERFGLTVTYLPVDQYALVSPEQVASALTEQTAMV
                     SLTYVNNEVGSILNVEDIVRAIRERERALGHRIAVHLDAVQAPGYAPLDVRTLGVDIM
                     SLSGHKFGGPVGTGVLYVRRATPFLPLIDGGVQERRRRAGTENVAGAVGLATALGLVT
                     SERPTVEPRIRRLRDRLIERITAGLPGAKLNGHPTERAANNVNFSFAGLDGESLVLAL
                     DAQGVACSAGSACAHTTWEPSHVLLAMGCTLEEATGALRLTLGNPTTDQDIDDAGRII
                     VDVVRRLLSDAYTLPSQGSEWAPPTEGEASPHGSAARLP"
     gene            983..1666
                     /gene="pth"
                     /locus_tag="VNL92_04805"
     CDS             983..1666
                     /gene="pth"
                     /locus_tag="VNL92_04805"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWO94063.1"
                     /translation="MNPHRLTTLPKGPLQLPRKALRRFRRAEEPERAVEWLIVGLGNP
                     GAHYAEHRHNIGFRVVNRLARRHDIDVKAKGKLAAIGQGEIEGLPVALAKPRTYVNRS
                     DEAIVELMRRYQVAPQRLLAVYDELDLPVGRVRIGRAGGHGGHNGMRPVVAAIGTDFP
                     RIRIGIGRPLVDGEPSWDPDVIAAYVLSQPPPEEREVLDAAVAKAAEAIESIVRDGVE
                     AAMNRFNRG"
     gene            1789..2976
                     /locus_tag="VNL92_04810"
     CDS             1789..2976
                     /locus_tag="VNL92_04810"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWO94064.1"
                     /translation="MTDVVIASAARTPIGSFGGQFAEVPAQALGAASIKEALRRAGLE
                     PSQVDEVIMGNVLQAGLGQNSARQAAIQAGLPETIPAMTINKVCGSGLKAVALAAQAI
                     ALGDASIVVAGGLENMTRAPYLLPTGRYGARLGHTEALDSMIVDGLWCAMSDCHMGVT
                     AENVAAKYEISRQEQDEFAARSQARAAEAMESGRFDDEIVKVEVPQRKGEPVIVAKDE
                     HPRPGTTVERLATLPAAFKKDGQVSAGNASGINDGAAATVVMSAEKAAELGIRPLAKI
                     RSYASAGVDPKVMGIGPVPASRKALARAQVDIGQIDLIEANEAFAAQSIAVARELQLD
                     LDKVNVNGGAIALGHPIGASGTRILTTLLYEMRRRGSHLGLATLCIGGGQGIAMVVEN
                     GSS"
     gene            complement(2973..>3133)
                     /locus_tag="VNL92_04815"
     CDS             complement(2973..>3133)
                     /locus_tag="VNL92_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HWO94065.1"
                     /translation="AAANRLQLIEAGVDAGRIDDLALCTGCRTDLFYSHRMEGEPTGR
                     FGASIVLV"
BASE COUNT          535 a         1014 c         1093 g          491 t
ORIGIN      
        1 ccaagccgtg ctgaacagct gccgctacct ggaacgattc ggcctgactg tgacctatct
       61 ccccgtggac cagtacgcgc tcgtttcgcc ggagcaagtg gcctccgcgc tgaccgagca
      121 gacggcgatg gtgagtctga cgtacgtcaa caacgaggtc ggcagcattc tgaacgtgga
      181 ggacatcgtc cgggcgattc gcgaacggga gcgtgcgcta ggacaccgca tcgctgtgca
      241 cctcgacgcg gtgcaggcac ctggatacgc gccgcttgat gtgcggacgt tgggggtcga
      301 catcatgtca ctctcaggcc acaagttcgg tggtccggtc ggcacgggtg tcctgtacgt
      361 ccggcgcgcg acgcccttcc tgccgctcat cgacggcggt gtccaggagc gtcgccggag
      421 ggcggggacg gagaatgttg ccggtgcggt aggcctcgca acggcgctcg gcctcgtgac
      481 atcggagcgc ccgacggtcg agccacgtat ccgccgcctg cgggaccggc tcatcgagcg
      541 cattacggct ggtttgccag gcgcgaaact caatgggcat ccgaccgagc gagccgcgaa
      601 caacgtgaac ttcagctttg ccggcctcga tggggaaagc ctcgtgctgg ctctcgacgc
      661 gcagggcgtg gcttgcagcg cgggtagcgc ttgcgcgcac acgacgtggg agccgtcgca
      721 cgtgcttctt gccatgggct gcacgctgga ggaggcgacg ggggcgctgc ggctcacgct
      781 cgggaacccc acgaccgacc aggacatcga cgacgccggt cgcatcatcg tcgacgtcgt
      841 gcgccgcctg ctgtcggacg cgtacacgct ccccagccaa ggttcggagt gggcgcctcc
      901 cactgagggt gaggcttcgc cccacggttc ggcggcgcga ctcccgtgac catttcgccc
      961 tcgccacttg gagtgtgggt ggatgaatcc gcaccggctc acgaccctgc cgaaggggcc
     1021 gcttcagctg ccccgtaaag ccctgcgccg gtttcgccgt gcggaggagc cggagcgcgc
     1081 cgtggaatgg ctcatcgtgg gcctcggcaa tccaggcgca cactatgccg agcaccgcca
     1141 caacatcggc tttcgcgtcg tcaatcgtct cgcacgaagg catgacatcg acgtgaaggc
     1201 gaaggggaag ctcgcggcca ttggccaggg ggagatcgag ggcctgccgg tcgcccttgc
     1261 caagccgcgg acctacgtga accgcagcga cgaagccatt gtcgagctca tgcggagata
     1321 ccaggtcgcg ccacagcgcc tcctcgcggt ctacgatgag ctggacttac ctgtcggccg
     1381 cgtgcgtatc ggcagggccg gcggtcatgg agggcacaat ggcatgcgcc ccgtcgtagc
     1441 ggccatcgga acggactttc cccgcattcg aattggcatt ggccgaccac tcgtggatgg
     1501 cgagccgagc tgggaccccg atgtcatcgc ggcgtacgtg ctgagccaac cgccaccgga
     1561 agagcgcgag gtgctcgatg cggcggtggc gaaggcggcc gaggcgatcg aatcgatcgt
     1621 ccgcgatggg gtcgaggcgg cgatgaatcg cttcaaccgc ggctgacggg gcgagctcag
     1681 cctcccagct tggctccgcg tgatatgatc cgtcacaact cagccggcat cggcgacggg
     1741 cgggccccgc tgtctgcctg ctcgcgctgg ccctcggagg ttccgaccat gaccgacgtt
     1801 gtgatcgcaa gtgccgctcg tacgccgatt ggttccttcg gggggcagtt cgccgaggtt
     1861 ccggctcagg cgcttggggc ggcttccatc aaggaagcgc tgcgccgcgc gggactggag
     1921 ccgtcgcagg tggacgaagt gatcatgggg aacgtgctcc aggcaggcct gggccagaac
     1981 agcgcccgtc aagccgccat ccaagccggc cttccagaaa cgatccccgc gatgacgatc
     2041 aacaaggtct gcgggtccgg cctgaaggct gtcgccctgg cggcgcaggc catcgcgctg
     2101 ggcgatgcca gcatcgtcgt ggccggaggg ctcgagaaca tgacgcgggc gccctacctg
     2161 ctcccaacgg gccggtacgg agcgcgcctg ggtcacacgg aagcgctgga tagcatgatc
     2221 gtcgatggcc tgtggtgcgc gatgagcgac tgccacatgg gcgtcactgc cgagaacgtc
     2281 gctgcgaagt acgagatatc gcgccaggag caggacgagt tcgccgcgcg gagccaggcg
     2341 cgagccgccg aagctatgga aagcggacgc ttcgacgacg agatcgtgaa ggtcgaggtg
     2401 ccacagcgga agggcgagcc ggtcatcgtg gccaaggacg agcatccgcg cccgggcacg
     2461 acggtcgaac gcttggccac gcttccggcc gcgttcaaga aggacggtca ggttagcgcc
     2521 ggtaacgcct ccggcatcaa cgatggtgcc gccgctaccg tcgtcatgag cgccgagaag
     2581 gcggccgagc tcggcatacg gccgctcgcg aagatccgct cctacgcctc ggccggcgtc
     2641 gatccgaagg tgatgggaat cgggccggtg ccggcttcgc ggaaggcctt ggctcgcgcc
     2701 caggtcgaca tcgggcagat cgacctcatc gaggcgaacg aggccttcgc agctcaatcg
     2761 atagccgtcg cgcgggagct gcagctcgac ttggacaagg tcaacgtcaa cggcggtgca
     2821 atcgcgctcg gtcaccctat cggcgcgagc gggacgcgca tcctcacgac cctgctctac
     2881 gagatgcgcc gccgaggctc gcatctcgga ctcgccacac tgtgcatcgg gggcggccag
     2941 ggcatcgcta tggtcgtgga gaacgggtcg agctaaacga ggacgatgct cgcgccaaac
     3001 cggcctgtcg gctccccttc catgcggtgg gagtagaaca ggtcggtccg gcatcccgta
     3061 cagagcgcca ggtcatcgat acgtccggca tcgacgccgg cctcgatgag ctggaggcgg
     3121 ttggcggccg cca
//
LOCUS       DATMBB010000216         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_8896, whole genome shotgun sequence.
ACCESSION   DATMBB010000216 DATMBB010000000
VERSION     DATMBB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8896"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VNL92_04820"
     CDS             complement(<1..594)
                     /locus_tag="VNL92_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94066.1"
                     /translation="MAEKLSITFLGSGNAFAPGRLWNAFLVNDRYLFDPSPAALPQMK
                     ALEKRPNDVELIFVSHFHADHWFGLPFLLLDYVYGDRRVPLTVIGPPGIEERVRMVTE
                     AGFPRVADEAATKFGVRWVEIDDGAEGEAGGVRYEVREVVHAKQLQCFGFRTRIAGRT
                     LAYSGDTILCQALVPLALGADVFVVECACWESACGPHL"
     gene            complement(622..1275)
                     /gene="thyX"
                     /locus_tag="VNL92_04825"
     CDS             complement(622..1275)
                     /gene="thyX"
                     /locus_tag="VNL92_04825"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWO94067.1"
                     /translation="MTVELLGHLGDDLAIVNAARVSYGRESRELTERDAGLIRFLARE
                     RHGTPFEHVVFTFRVTTSIAVAREWFRHRMGSFNEISTRYVEMNDEFYLPPPENVRRQ
                     VGKPGAYHFEPVAEDEARAAIEAMRSAYEHAYQAYKELLDSGVAKELARNVLPLGMLT
                     QFIWTVNFRSVTNFLSLRTASNALLEIRQEAQQVEELVEKVLPHAYAAWVEGGRASL"
     gene            complement(1272..1634)
                     /locus_tag="VNL92_04830"
     CDS             complement(1272..1634)
                     /locus_tag="VNL92_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94068.1"
                     /translation="MTSEQHAPVPELRLAAFQRDVHETARGRGWWDEERSPLEAIALI
                     HAELSEAVEEYRASHGSISATRIENGKEEGFGVEMADAIIRILDLAEHLGIDLESILL
                     RKAAYNKTRPYRHGGKRA"
     gene            complement(1631..2221)
                     /gene="dcd"
                     /locus_tag="VNL92_04835"
     CDS             complement(1631..2221)
                     /gene="dcd"
                     /locus_tag="VNL92_04835"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWO94069.1"
                     /translation="MLSNRDIRRRIEAGDLIIDPPPADAAFGGASVDLTLSTNIRIIN
                     PSRCAYIDYARIQDDPEYLTSITDAVDLAEGQPLILHPGRVVICSTVERVRVPVDICA
                     ELHGRSSLARLGVLPHTAGKVDPGWDGTLTLEVSNLGEIPVALYPGEAVCQMVFHELS
                     SPADLSSPRAVSRYQGQTGPTLTSPRRDGIPTVWPP"
     gene            2506..>2749
                     /locus_tag="VNL92_04840"
     CDS             2506..>2749
                     /locus_tag="VNL92_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO94070.1"
                     /translation="MYDLSLRSKVFIMTGVMLGLLLSALDQTIVSTAMPRIIASLGGI
                     EYYSWVVTAYLLASTASVPIFGKLSDIYGRKPFYLFG"
BASE COUNT          480 a          905 c          889 g          475 t
ORIGIN      
        1 gaggtgaggg ccgcacgcgc tctcccagca ggcacactcg acaacgaaga cgtcggcgcc
       61 gagcgctagg ggcacgaggg cctggcagag gatcgtgtcg cccgagtagg cgagggttcg
      121 tccggctatc cgcgtccgaa agccgaagca ttgcaactgc ttcgcgtgga cgacctcgcg
      181 cacctcgtac cgcacgccac ctgcctcgcc ctcggccccg tcgtcgatct cgacccaccg
      241 cacgccaaac ttggtcgccg cctcgtcggc gacacgcggg aagcccgctt cggtcaccat
      301 gcgcacacgc tcctcgatac caggcgggcc gatcacggtg agcggcaccc tgcgatctcc
      361 gtagacgtag tcgagcagca ggaacggcag tccgaaccag tggtcggcgt ggaagtgact
      421 gacgaagatg agctcaacat cgttcgggcg cttctccagc gccttcatct ggggaagggc
      481 ggcaggcgat ggatcgaaga ggtagcgatc gttcacgagg aaggcgttcc acaggcgccc
      541 gggcgcaaag gcgttgccgg agccgaggaa cgtgatgctc agcttctcag ccacgccacg
      601 acccgccgcg ccatgctttc atcagagcga tgcccggccg ccctcgaccc atgcggcata
      661 ggcgtggggc agaaccttct ccacgagctc ctccacctgt tgcgcctcct gccggatctc
      721 gagcaaggcg ttcgaggccg ttcgcaggct caggaagttc gtgaccgagc ggaagttcac
      781 ggtccagatg aactgggtca gcatcccgag cgggagaacg ttcctggcga gctccttcgc
      841 gacgccagaa tcgagcagct ccttgtacgc ctggtatgcg tgctcgtagg cggaacgcat
      901 agcctcgatg gccgcccgtg cctcatcctc cgcgacgggc tcgaagtggt atgcgcccgg
      961 cttgccgacc tgccgtcgca cgttctccgg gggcggaagg tagaactcgt cgttcatctc
     1021 cacgtagcgc gtgctgatct cgttgaacga tcccatgcgg tggcgaaacc actcgcgggc
     1081 cacggcgatg gacgtcgtca cccggaacgt gaagacgaca tgctcgaagg gcgtgccgtg
     1141 ccgctcgcgc gccaggaagc ggatcagccc agcatcgcgt tccgtcagct cgcgcgactc
     1201 gcgcccgtac gaaacgcgcg ccgcgttgac gatcgcgagg tcgtcgccga ggtgtccgag
     1261 cagctccacg gtcacgcgcg cttgcctcca tgccggtaag gacgtgtctt gttgtacgcg
     1321 gccttccgca acaggatcga ctccaggtcg atgccaaggt gttccgcgag gtcgaggatg
     1381 cggatgatcg catccgccat ctcgactccg aagccctcct ccttgccgtt ctcgattctc
     1441 gtggccgaga tgctcccgtg ggaggcgcga tattcctcca ccgcctccga cagctctgcg
     1501 tggatgagcg cgatggcttc gagcggcgac cgctcctcgt cccaccagcc acggccccgc
     1561 gccgtctcgt ggacgtcgcg ctgaaacgcc gcgagccgca gctccggaac gggcgcgtgt
     1621 tgttccgatg tcatggcggc catacggtcg gaatcccatc ccggcgcggc gaggtgagcg
     1681 tgggcccggt ctggccctgg taccgcgaca cggccctagg cgacgagagg tcggcggggc
     1741 tgctgagctc gtggaagacc atctggcaca ccgcttcccc cggatacagg gccacgggga
     1801 tctcgccgag gttgctgacc tccaacgtga gcgttccatc ccaacccggg tcgactttgc
     1861 ctgccgtgtg cggcagcacg ccgaggcgcg cgaggctgct gcgaccgtgc agctcggcgc
     1921 agatgtcgac cgggacgcgc acgcgctcga ccgtcgagca gatgaccacg cgcccgggat
     1981 gcaggatgag gggctgcccc tcggcgagat cgacggcatc cgtaatggac gtgaggtatt
     2041 ccgggtcgtc ctggatgcgg gcgtaatcga tgtaggcgca ccggctgggg ttgatgatcc
     2101 tgatgttcgt tgacagcgtg agatcgacgg aggccccgcc gaaggccgcg tcggcgggcg
     2161 gcgggtcgat gatgaggtcg ccggcctcga tgcgtcggcg gatgtcgcga ttggacagca
     2221 tctcgttccg ttacccccgc gcgcggatcc cgagcgaagg ctagcagccg ggcgcattac
     2281 acggcaagtc gattatgcaa agcgcgacga accagcgccc ctcgatagtg agttacagga
     2341 aggtcagcag gcaaacaatt tcgcctcacc ctacttgttc ggacgatacg tgccgatcta
     2401 cagtatgaaa gctacagcac tgcacacagc cggacgcgcg tgcccggcgc tccctccaag
     2461 agcacagcaa gagttctgcc atcacgtata gaggttactt ggcgcatgta tgatctaagc
     2521 ctgcgtagca aagttttcat catgaccggc gtcatgctcg gtctcctgct ctccgccctg
     2581 gaccagacga tcgtgagcac ggcgatgccg cgaatcatcg catcgctggg cgggatcgag
     2641 tactacagct gggtcgtgac ggcgtacctt ctcgcctcga cggcatcggt acccatcttc
     2701 ggcaagctgt ccgacatcta tggccgcaag ccgttctatc tgtttggcg
//
LOCUS       DATMBB010000217         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_87033, whole genome shotgun sequence.
ACCESSION   DATMBB010000217 DATMBB010000000
VERSION     DATMBB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87033"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VNL92_04845"
     CDS             complement(<1..658)
                     /locus_tag="VNL92_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94071.1"
                     /translation="MPRKLVEAAFRHKLLLALPIVLVPALVFLFYPSPEDEYVSETTV
                     WVETPSFAAQVTQPAPVYPILTPAQQQAEVLRQMLTTRSFRENAAIDAKLVTPEAPEA
                     DRSEAATEIGERISIVERGENLIAIEARGTSPQHAQALALGFLKEYNDRVIFEAGRTT
                     QLTTDYYMGQLNLAQAELAKRRQEAQAYLTAHPKAYESKAPDFTYDSLIARVNEQAAV
                     V"
     gene            complement(767..1447)
                     /locus_tag="VNL92_04850"
     CDS             complement(767..1447)
                     /locus_tag="VNL92_04850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWO94072.1"
                     /translation="MTTTSVADPVASPPQQIAVPGVLERLAPMPWWKRSIDIVAASIG
                     LVLLLPIFAVIALAIVIDSRGGPLYRQTRVGRGARTFRCWKFRTMHVNADQALASLLE
                     HNEANGHIFKMKDDPRVTRVGRLLRKTSLDELPQLWNVLRGNMSLVGPRPPLVSEVVR
                     YEARELRRLTATPGITGLWQVTARDRHDFEDMVELDAEYAERLGFWLDVRILLKTIPT
                     VLLGRGSC"
     gene            complement(1444..>2019)
                     /locus_tag="VNL92_04855"
     CDS             complement(1444..>2019)
                     /locus_tag="VNL92_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HWO94073.1"
                     /translation="QALAHTAAVHGAHPQLLRSVMDINRDQRLRVVQKLTEVLGGLEG
                     RRIGILGAAFKPHTDDLRYSPAIELAALLCIEGAQVAVYDPVVSADAIERSGCGAKAS
                     ANVESAAIGAQALVLATEWPEFRDLPFARLAQLMAYPVLVDARNFLDGAVLRGAGFVY
                     RSIGRPASDGGPLPAERDVWPSELAATVAAR"
BASE COUNT          349 a          641 c          678 g          351 t
ORIGIN      
        1 cgaccaccgc cgcttgctcg ttgacgcgcg cgatgaggct atcgtacgtg aagtccggag
       61 ccttgctttc gtacgccttg ggatgcgccg tgaggtaggc ttgcgcctcc tgccgacgct
      121 tagcgagctc ggcctgggcc aggttcagtt gccccatgta gtagtccgtc gtcagctggg
      181 tcgtccgtcc ggcctcgaag atcactcggt cgttatattc cttcaggaag ccgagggcga
      241 gggcctgcgc atgttgcggc gacgtgccgc gcgcctctat cgcgatgagg ttctccccgc
      301 gctcgacgat ggagatccgc tccccaattt cggtcgctgc ttcggaccgg tcagcttccg
      361 gcgcctccgg cgtgaccagc ttcgcatcga tcgcggcgtt ctcgcggaac gatcgcgtcg
      421 tcagcatctg ccgcaggacc tccgcctgct gctgggccgg cgtaaggatc ggatacacgg
      481 gcgccggctg cgtcacttgc gccgcaaacg agggagtctc gacccacacg gtcgtctccg
      541 agacgtactc gtcctctggg ctcggataga acaggaatac gagcgccgga acgaggacga
      601 tgggaagggc gaggagcagc ttatgccgaa acgcggcctc tacgagcttt cttggcaccg
      661 gtgtaacctc cgaggctgga actacggtcc gttcggccta ggaagaagat aggccccggt
      721 ggtggagcag gagttaagga aagggtgagg gagcgacgga cgaggattaa caggagcccc
      781 gtccgaggag cacggtcgga atcgtcttga ggaggatgcg aacgtcaagc cagaacccca
      841 ggcgctcggc gtactccgcg tcgagctcga ccatgtcttc gaagtcgtgt cggtcgcgtg
      901 ccgtcacctg ccagaggccc gtgatgccgg gggtggcggt gagccggcgc agctctcgcg
      961 cctcatagcg gacgacttcg ctcacgaggg gcgggcgcgg tccgacaagg ctcatgttcc
     1021 ctcgcagcac gttccaaagt tgcggcagct cgtcgaggct cgtctttcgt agaaggcggc
     1081 cgacgcgtgt gacccgtggg tcgtccttca tcttgaagat gtggccgttc gcctcgttgt
     1141 gctctagcaa gctcgccagc gcctggtccg cgttgacgtg catggttcgg aacttccagc
     1201 agcggaaggt ccgcgcgcct ctgccaactc gtgtctgtcg atagagcgga ccgccccggc
     1261 tatcgataac gatggccaag gcgatgacgg caaagatcgg caggaggagc acgagtccaa
     1321 ttgacgctgc cacgatgtcg atcgaccgct tccaccacgg catcggtgca aggcgttcca
     1381 gaacgccggg aactgcgatc tgctgtggcg gacttgcgac cgggtccgcg acggatgtcg
     1441 tcgtcatcgc gccgccaccg tcgccgcgag ctcggatggc caaacatccc gctcggccgg
     1501 cagcggtccc ccatcggatg cagggcgacc tatcgagcga tagacgaagc cggcgccgcg
     1561 aagcacggcg ccatcgagga agttgcgcgc gtcgaccaac accgggtacg ccatgagttg
     1621 cgcaaggcga gcgaaaggaa gatcgcgaaa ctccggccac tcggtcgcga ggacgagcgc
     1681 ctgcgcaccg atggcggcgc tctccacatt cgcggaggcc ttcgctccgc acccagagcg
     1741 ctcaatcgca tcagcagata cgaccgggtc gtacacggcg acctgcgctc cttcgatgca
     1801 gagcagcgcc gcgagctcaa tggccggcga gtatcgtaga tcgtcggtgt gcggcttgaa
     1861 cgcggccccg agtatgccga tccgccggcc ctcgagcccg ccgagaacct cggtgagctt
     1921 ctgcacgacg cgaagccgct ggtcgcggtt gatgtccatg accgagcgga ggagctgcgg
     1981 atgtgccccg tgcaccgctg cggtgtgcgc aagcgcttg
//
LOCUS       DATMBB010000218         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_24440, whole genome shotgun sequence.
ACCESSION   DATMBB010000218 DATMBB010000000
VERSION     DATMBB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24440"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..508)
                     /gene="crcB"
                     /locus_tag="VNL92_04860"
     CDS             complement(161..508)
                     /gene="crcB"
                     /locus_tag="VNL92_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWO94074.1"
                     /translation="MAGSLGAVSRYAVDAWANDHLGGPFPWGTLIVNLSGSFAFGLVF
                     TLVTERLDPHPDVRFALTTGFLGAYTTFSTFAFETYRLADRGNVALAIVNVIGSIGAA
                     LMAVWLGIRLARA"
     gene            complement(543..1295)
                     /locus_tag="VNL92_04865"
     CDS             complement(543..1295)
                     /locus_tag="VNL92_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HWO94075.1"
                     /translation="MRQTTELIEIRVTPLRVPDLAASLVAEFLGPFTFIFAGIAATAV
                     ANEGAGNLITIGLAYGLAIGLMVAAMGHISGAHFNPAVTIGMFLAGKIDLVKGVLYIV
                     AQLAGTAAAAGLLKYILNDLPFDAVKAGVPALSGAVDEGQGLLLEIIMTFFLVMVIFG
                     TAVDPRAGHRAVAGLAIGLAVTMDTFLGGPLTGAAMNPARSFGPAAVANEWADAWIYW
                     VGPIVGAALAALLFSTVLMSAEEEAVPFEQPV"
     gene            complement(1412..>2344)
                     /locus_tag="VNL92_04870"
     CDS             complement(1412..>2344)
                     /locus_tag="VNL92_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94076.1"
                     /translation="QVLDPGPAGRQHISDRNGDGTITLGCLGDSNTREIALGGNTFLG
                     WCRIIDAQLATLGGRWSAINLGVPAATAVRFADWPSAYDQLDLALQREDADIVVLSFG
                     SGDIPFVTAAARPLTVAYACQEGALMADLSWSPASDQADIWVDEGGDGEFEQFIDVSP
                     HQSSLRLEAGQARRVDYFLIGEDLRTITANRGRASPTCSDQPATFAPTASRQLTLDDI
                     VAAYLDLVSTAASQNVPAYVALTPLYNNGDWRQNRLVLELNNMLDHELPNAALIDTAS
                     IMTEDDYYDALHANQRGQSRRAEAIFRAIAISFR"
BASE COUNT          422 a          716 c          806 g          400 t
ORIGIN      
        1 acgaagtcgt cacgaagctc ctcgccctgc acgaggccgg tgtcgagtac ttcgtcatca
       61 acatgcccga ttcctatacg ctggaaccgc tgcggctgtt cgcccgtgag gtgatgccgg
      121 cgctcggtga actgccgcta cgctgaaccg cgtcgcctcc ttacgctctc gccaggcgga
      181 tgccgagcca taccgccatc agcgccgcgc cgatgctacc aatcacgttg actatggcga
      241 gggcgacatt gccgcggtcg gcgaggcggt acgtctcgaa ggcgaacgtg gagaacgtcg
      301 tgtacgctcc gagaaagccg gttgtgagcg cgaaacgaac gtcggggtgc ggatcgagcc
      361 gttcggtcac gagcgtgaac acgagtccga aggcgaagga gccgctgagg ttcacgatca
      421 gcgtacccca ggggaaaggc cctccgaggt ggtcgttggc ccaggcatcg acggcgtagc
      481 gagagacggc gccgagcgat ccggccaagg cgatccatat cagacgcatc cggaggcgcc
      541 gatcagacgg gctgctcgaa tggaacggct tcctcctcgg cgctcatgag cacggtgctg
      601 aacaggagag ccgcgagggc cgccccgacg atcggaccaa cccagtagat ccaagcgtcg
      661 gcccattcgt tcgcaacggc cgcggggccg aaggaacgtg ccgggttcat ggccgcgccg
      721 gtaagcggac cgcccaggaa ggtatccatg gtgaccgcca ggccgatggc gagtcccgcg
      781 acggcgcgat ggccggcgcg gggatcgacc gctgtcccga agatcaccat caccaggaag
      841 aacgtcatga tgatctcgag aaggagccct tgtccctcgt cgaccgcgcc gctgagtgcc
      901 ggtacgccgg ccttgacagc atcgaacggg agatcgttga ggatgtactt gaggaggccc
      961 gcggccgcgg cggtgccggc aagctgggcc acgatgtaga gcacgccctt cacgaggtcg
     1021 atcttccctg cgaggaacat gccgatggtc actgctggat tgaagtgggc gccggagatg
     1081 tggcccatag cggcgaccat gaggccgatg gcgaggccgt atgccagacc gatcgtgatg
     1141 aggttacccg cgccctcgtt cgcgacagcc gttgcggcga tacccgcgaa gatgaacgtg
     1201 aacggtccca ggaactcggc gacaaggctt gcggcgaggt cgggcacgcg caatggtgtc
     1261 acccgtatct cgataagctc tgtggtttgg cgcacggcag tctcccccaa atctgcttaa
     1321 gggcccacct ccatgtacat tcgcacaata ggatgctccg ccaccgtcgt caacggccaa
     1381 caagcagcag tgcgtcagat ggttcgcatt cttaccgaaa gctgatggcg attgccctga
     1441 agatggcctc cgcacggcgc gactgcccgc gctggttggc gtggagagcg tcgtagtagt
     1501 cgtcctcggt catgatcgaa gccgtgtcga tgagcgcggc gttcggcagc tcgtggtcga
     1561 gcatgttgtt gagctcgagg acgagccggt tctggcgcca gtcgccattg ttgtacaggg
     1621 gcgtcagcgc gacataggcc gggacgttct gactcgcggc cgtgctgacc agatccaggt
     1681 aggcggcgac gatgtcgtcg agtgtgagtt gtcgtgaggc tgtgggggcg aaggtcgccg
     1741 gttggtcgct acacgtgggg ctggcgcggc ctcggttcgc cgtaatcgtc cgcaaatcct
     1801 cgccgatgag gaagtagtcg actcggcgtg cttggccggc ctcgaggcgg agcgaggatt
     1861 gatggggcga cacgtcgatg aattgctcga actcgccgtc cccgccctca tcgacccata
     1921 tgtctgcctg atcgctcgcg ggcgaccacg agaggtcggc catcagcgcg ccctcctgac
     1981 acgcgtatgc gaccgtcagt gggcgcgccg ccgccgtgac gaatggaatg tcgcccgacc
     2041 cgaagctgag gacgacgatg tcggcgtcct cgcgttggag cgcgaggtcg agctgatcgt
     2101 atgcactggg ccaatcggcg aatcggaccg ctgtggcggc aggcacgccg aggttgatgg
     2161 cgctccagcg ccctccgagc gtggcgagct gcgcatcgat gatgcggcac catccgagga
     2221 atgtattgcc cccgagcgcg atctcacgcg tgttggaatc gccgaggcag ccgagtgtga
     2281 tggtaccgtc cccgttccgg tccgaaatgt gctgccgtcc tgctgggccg gggtcgagga
     2341 cctg
//
LOCUS       DATMBB010000219         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_468179, whole genome shotgun sequence.
ACCESSION   DATMBB010000219 DATMBB010000000
VERSION     DATMBB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468179"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VNL92_04875"
     CDS             <1..213
                     /locus_tag="VNL92_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWO94077.1"
                     /translation="GLAILCPITSQVKGYPFEVPIPAGLPVGGVVLSDQAKSLDWRAR
                     KAEFICTLPRETTQEILLKLATLLTP"
     gene            395..955
                     /locus_tag="VNL92_04880"
     CDS             395..955
                     /locus_tag="VNL92_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWO94078.1"
                     /translation="MGLLTLSINVTLDGCVDHREGIADDETHAFFTRLMDECGAMLWG
                     RVTYEMMESYWPAVARGDVEAPPAMREWAVKLEAKPKYVVSSTRKDYPWTNSHHIVGD
                     LRTGVQRLKDATPAGVLLGSGRLATELDRLDLIDEYKFLVHPRIAGHGPTLYQSGLPS
                     TRWLELVSTKPLRSGAVTMHYRRARG"
     gene            1042..2136
                     /locus_tag="VNL92_04885"
     CDS             1042..2136
                     /locus_tag="VNL92_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94079.1"
                     /translation="MDYPRKDADGKTGSNLDGEPSRLLFEAELRKHVALHDKLARDYL
                     KQYRKLLTKKDLRLVAKITSDVADQWRHAILHSEGDSARIAQLAAAARRKLDRAVSRA
                     LPGVAKARALRRAQMREHSKLSAATLAFHGAGHHVDLGDLTATLTDCAEFVPPFTSFD
                     VVSVAGPGDYIVRDDSFAKPGIGHLVNNFDYDQNENTSFSDGLWGLLTPAFGSNSAAC
                     GVAFTAPRDGRLEIHAVMRNFYNKVMFSVTDKFGFSDAEVDFFLQLFIMVVRGSQVIF
                     MPTTLRHNGLVSHGSNLSFHESPIDNSVPVTISAITDQRFNANESVLVLAGSRVLVST
                     VIDDMHARVNAVLWWQLQKLCIKTADDVFT"
     gene            2676..>2999
                     /locus_tag="VNL92_04890"
     CDS             2676..>2999
                     /locus_tag="VNL92_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94080.1"
                     /translation="MKVTLFISRAENQPGHYLVVVNGEGFFESVNRSVGARIRGDDPW
                     FDDRLFSIGGTGIERVGPDGSFSLSRIVSGSQLNEDWGRDEIYALVDVEGLSGTFKSN
                     TVVGHF"
BASE COUNT          566 a          936 c          966 g          531 t
ORIGIN      
        1 ggcctcgcta tcctctgccc catcacgagc caagtgaagg gctatccctt tgaggtcccc
       61 atcccggccg ggctccccgt cggtggagtt gtactctcag atcaagccaa gagcctcgat
      121 tggagagcaa ggaaggccga attcatatgc acccttccgc gggagacaac ccaagaaatt
      181 ctcctcaaac tcgcaaccct cctcacgcca tagagacgca tctcagggtg cctaacaact
      241 ggtggagccg ccccggccag cccggagcca gcatttcatg cgatactagt cctgggctgg
      301 ccgcgcagct catctcgagg ccgttagccg ctgcgaggta cttgtcgaaa gtgacagttc
      361 ggcggtccgc ccaacttgga gtagacttga cgtcatggga ctcttgaccc tcagcatcaa
      421 cgtcaccctc gacggttgcg tcgaccaccg ggagggaatc gccgacgacg agacacacgc
      481 gttcttcacc cgcctcatgg acgagtgcgg ggcgatgctg tggggccgcg tcacctacga
      541 gatgatggag agctactggc cagcggtggc ccgcggcgac gtggaggcgc cgccggcgat
      601 gcgcgagtgg gcggtcaagt tggaggccaa gccgaagtac gtggtgtcat cgacacgaaa
      661 ggactaccca tggaccaaca gccaccacat cgtcggcgac ttgcgcacgg gcgtgcagag
      721 gctcaaggac gcgaccccgg ccggcgtgct cctcggtagc ggcaggctcg cgaccgagct
      781 ggaccggctg gatctgatcg acgagtacaa gttcctcgtc caccccagaa tcgccggcca
      841 cggcccgacc ctctaccaga gcgggctgcc cagcacacga tggcttgagc tggtttcgac
      901 gaagccactc cgcagcggcg cggtcacgat gcactatcgg cgcgcgcgcg gctagcaacc
      961 gggtgcagcg gacggtccgc tgcgcggccc gccgctgaac cggagcgtca gcgttttcgc
     1021 agtatctcaa ggaggcactc agtggactat cctcggaagg atgccgacgg gaaaaccggt
     1081 tccaacctgg acggcgagcc ctcgcgtctc ctgttcgaag ccgagcttcg caaacacgta
     1141 gcgctccacg acaagctcgc gcgcgactat ctgaagcaat acaggaagct gctgacaaaa
     1201 aaggacctcc ggctcgtggc gaagatcacg agcgacgtcg ccgaccagtg gcggcacgcc
     1261 atcctccaca gcgagggcga ctcggctcgt atcgcgcagc tcgcagccgc cgcgcggcgg
     1321 aagctcgatc gggccgtgag ccgcgcgctg ccaggtgtcg ccaaggcaag agcgctgcgc
     1381 cgggcgcaga tgcgcgagca cagtaagctg tcggcggcta cgcttgcctt tcacggcgcc
     1441 gggcaccatg tagacctcgg cgatctcacg gcgacgttga ccgactgcgc ggagttcgta
     1501 ccgcccttca cgtcgttcga cgtggtctcg gtcgccggcc ccggcgacta catcgtgcgc
     1561 gatgactcct tcgcgaagcc gggtatcggt caccttgtga acaatttcga ttacgatcag
     1621 aacgagaaca cgtcgttcag cgacgggctg tggggactcc tgacccccgc gttcggatct
     1681 aactcggctg catgcggcgt ggcgtttacg gcgccgcggg acggccggct cgagatccac
     1741 gcagtaatgc gcaacttcta caataaggtc atgttctcgg tcactgacaa gttcggcttc
     1801 tccgacgccg aggtcgactt tttcctgcag ttgttcatca tggtcgtgcg tggctcgcag
     1861 gtcattttca tgccaacgac gctgcggcac aacgggctcg tctcccacgg cagcaacttg
     1921 agctttcacg agtcgcccat cgacaactcg gtgccggtga cgatcagcgc cattaccgat
     1981 caacgcttca acgccaacga atcggtgctc gtccttgcgg gatcgcgagt gctcgtatcg
     2041 accgtgatcg acgatatgca cgccagggtg aatgccgttc tctggtggca gcttcagaaa
     2101 ctgtgcatca agaccgccga cgacgtgttt acctgattgt cgaagttggg tcgataggcg
     2161 ttacctagcg cgagcccggt agccaactac ctcgccgtct cgcggtgaag ggaggtgcgg
     2221 ggagggatcg cccgggtgct gctgcgaggc cgagcgatcg ccatggacgc tgccgtggtc
     2281 ggggagcccc ggggggggcg gtggattcag cccgccaacg ctggcatttc cgttcggttc
     2341 agcgaccttc cgtcagctag gttcaaggcg tggctgaggc gcttactgac ggtacgcatc
     2401 cggacggggt gtcaaccaaa ccggggcaag atccccgggc cggctgcggc gaggcgagtt
     2461 cgtgctcgaa ccgaatcgcc atattcgatc cggagacgcg gtgtccgggt ggcgattgaa
     2521 gacagtctcc tcgacctggc aggcgtccgt gaagtcggcg ggggtggatg gtcggcaccc
     2581 tgggcgaacg aagtcgctcc agccttgaca tgggagggtg gtaggcgcag agttaccgta
     2641 cagcgttcac gacagtcccg acggagggac ctcatatgaa ggtaacgctc ttcatcagcc
     2701 gagccgagaa tcagccgggg cactacctcg tggttgtcaa cggcgaaggg ttcttcgagt
     2761 cggtcaacag gagtgtcggc gctcggattc ggggcgacga cccgtggttc gatgacaggc
     2821 tcttcagcat tggcggcacc ggcatcgaac gcgtcggccc tgacgggagc ttcagcctgt
     2881 ccaggatcgt ttccggcagc cagctcaacg aagactgggg ccgggacgag atttacgccc
     2941 tcgtcgatgt cgaagggctc agtgggacgt tcaagagcaa tacggtcgtg gggcacttc
//
LOCUS       DATMBB010000220         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_560720, whole genome shotgun sequence.
ACCESSION   DATMBB010000220 DATMBB010000000
VERSION     DATMBB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560720"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2264
                     /locus_tag="VNL92_04895"
     CDS             <1..2264
                     /locus_tag="VNL92_04895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWO94081.1"
                     /translation="GRFVRQDIEDSKGKVLLERDAEVTDQALAKLRKAGVERVPVRPF
                     VTSQIEYLAADEEDRYVIAQANAVLDDRGHFVEGRIEVRLGDRFLMEAPENVEYMDVS
                     PKQIVSVAASLIPFLEHDDANRALMGSNMQRQAVPLLRPEVPLVSTGMERQAARDSGQ
                     VVLAHRGGVVVGASSKEIVVRTDEGETDRYELLKFVRSNQGTCINQRPIVRPGDRVEP
                     GQPLADSSSTDGGELALGQSVLCAFMSWEGYNFEDAIILSEALVRDDKFTSIHIEKYE
                     TEARDTKLGPEDITRDIPNVGEESLRDLDENGIIRIGAEVTAGDILVGKITPKGETEL
                     TAEEKLLRAIFGEKAREVKDTSLRVPHGERGKVIDVKVFSRENHHELPAGVNQLVRVS
                     IAQRRKVSEGDKMAGRHGNKGVVARVLPVEDMPYLPDGTPVDIILNPLGVPSRMNVGQ
                     VLETHLGWAAQTLGFRAITPVFDGASGESIEDALARAWIGVESGAIEVDSDERHQNGA
                     RIGERFDLEKARRWLGERGYNPDEILDDARPGTAARACLRMWLEAQGESNVRDLDLEG
                     LRVRAEEVGRRKHLAPPVLEKQILYDGRTGEPFDQPVTVGYIYMMKLVHLVEDKIHAR
                     STGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAHVLQELLTVKSDDVVGRVKT
                     YEAIVKGEDVLEPGVPESFKVLVKELQSLGLAVEVFNEDEERVTLAETSAVDVPELDI
                     NLSGFEREDPFSA"
     gene            2257..>2882
                     /locus_tag="VNL92_04900"
     CDS             2257..>2882
                     /locus_tag="VNL92_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWO94082.1"
                     /translation="MLEVNDFNAIRISLASPEQIMSWSYGEVTKPETINYRTLKPEKD
                     GLFCEKIFGPTKDFECYCGKYKRVRYKGIVCDKCGVEVARSKVRRERMGHIALATPVS
                     HIWFVKGTPSRMGLLLDLSPRSLEKVLYFAQYIVTSVDERARERALEVLREDLDREAM
                     TRERPHQNELDALMNKRDADLVAVESERASRLAQVDDDLSAQVDEVME"
BASE COUNT          563 a          834 c          987 g          498 t
ORIGIN      
        1 ttggccgatt cgtgcgccag gacatcgagg attcaaaggg taaggtgctg ctcgaacgtg
       61 atgcggaagt gaccgaccag gcgcttgcca agctccgcaa ggccggcgtc gaacgcgtgc
      121 ctgttcgacc gtttgtcacc agccagatcg aatacttggc ggccgatgag gaggatcgct
      181 acgtcatagc gcaggcaaac gccgttctcg acgaccgcgg ccatttcgtg gagggccgca
      241 tcgaggtgcg cttgggcgac cggttcctca tggaggcgcc ggagaatgtg gagtacatgg
      301 acgtgtcgcc gaagcagatc gtttcggtgg cggcgtcgct cattccgttc ctcgagcacg
      361 acgacgcgaa ccgcgcgctc atgggttcga acatgcagcg tcaggcagtg ccgctgcttc
      421 gaccggaagt tccgttggtc agcaccggta tggagcgcca agcggcccgc gattcggggc
      481 aggtcgttct ggctcaccgc ggcggtgtcg tggtcggtgc ctcctcgaag gagatcgtcg
      541 ttcgtacgga cgaaggggag accgatcggt acgagctact caagttcgtg cgctcgaatc
      601 agggaacgtg catcaaccag cgacccatcg ttcgcccggg cgatcgtgtc gagccgggcc
      661 agcccctggc agatagctcg tccacggatg gaggggagct tgcgctcggt cagagcgtcc
      721 tctgcgcgtt tatgagctgg gaaggctaca acttcgagga cgcgatcatc ctctcggagg
      781 cgctggtccg cgacgacaag tttacgtcga ttcacatcga gaagtacgag acggaagcgc
      841 gcgatacgaa gttgggtccg gaggacatca cgcgggacat cccgaacgtc ggcgaggagt
      901 cgcttcgcga tctggacgag aacggcatca tccgcattgg ggccgaagtg acggccggcg
      961 acatcctcgt cggcaagata actccaaagg gcgagacgga gctgaccgcc gaggagaagc
     1021 tgctccgcgc catcttcggc gagaaggctc gcgaagtgaa ggacacctcg ctccgagtcc
     1081 cccacggcga acggggcaag gtcatcgacg tcaaggtgtt ctcacgtgag aaccaccacg
     1141 agctgcctgc gggcgtgaat caacttgtgc gcgtatccat cgcccagcgc cggaaggtgt
     1201 cggagggcga caagatggcc ggtcgccacg gcaataaggg ggtcgtggcc cgcgtgctgc
     1261 cggtcgagga tatgccgtac ctgcccgatg gcacgccagt cgatatcatc ctgaatccgc
     1321 tcggcgtacc gagccgcatg aacgtcgggc aagtgctcga gacccacctc ggctgggctg
     1381 cccagaccct cggattccgc gcgatcacgc ctgtgttcga tggcgcgagc ggcgaatcga
     1441 tcgaagacgc cctcgctcga gcctggattg gtgtcgagtc cggcgcgatc gaagtcgact
     1501 cggacgagcg gcaccagaac ggcgcgcgca tcggcgagcg gttcgatttg gagaaggccc
     1561 gcaggtggct tggggagcga ggctacaacc cggacgagat cctcgatgac gcgcgtccgg
     1621 gcacggcagc ccgcgcttgc ttacgcatgt ggctcgaggc ccaaggtgag agcaacgtcc
     1681 gggacttgga cctggaaggg ctgcgtgtcc gagcggagga agtcggtaga cgcaagcact
     1741 tggcgccgcc tgtgctcgag aagcagatac tctacgacgg tcgcaccggc gagccgttcg
     1801 atcagccggt gacggtcggc tacatctaca tgatgaagct tgtgcacctt gtcgaggaca
     1861 agattcacgc tcgctccacc ggtccgtact cgctgattac ccagcagccg cttggcggaa
     1921 aggcgcaatt cggtggccag cgtttcggcg aaatggaggt gtgggcgctc gaggcctacg
     1981 gcgccgcaca tgtgttgcag gagctgctca ccgtgaagag cgatgacgtg gtgggccgcg
     2041 tgaagaccta cgaggccatc gtgaagggcg aggacgttct ggaaccgggc gttcccgagt
     2101 ccttcaaagt gctcgtgaag gaattgcaga gcctcggctt ggccgtcgag gtgttcaacg
     2161 aagacgagga acgtgtgacg ctcgccgaga catcggcggt cgacgtgccg gagctggaca
     2221 tcaacctttc tggcttcgag cgggaggatc ccttcagtgc ttgaggttaa cgatttcaac
     2281 gccatccgta tttccttggc ctcgcctgag cagatcatgt cgtggtcgta cggtgaggtc
     2341 accaagccgg agacgatcaa ctaccgcacg ctcaagccgg agaaggatgg cctgttctgc
     2401 gagaagatct tcggtccgac gaaggacttc gagtgctatt gcggaaagta caagcgtgtc
     2461 cgttacaagg gcatcgtctg cgacaagtgc ggtgtggaag tggcacggag caaggtacgc
     2521 cgcgagcgga tgggtcacat cgccctggcc acgccggtca gccacatctg gttcgtcaag
     2581 ggcacgccga gtcgcatggg gctcctgcta gacctgtcgc cccgcagcct cgagaaggtg
     2641 ctgtacttcg cgcaatacat cgtgacctcc gtggatgagc gggcgcggga gcgcgcactc
     2701 gaggtgctgc gcgaggacct ggaccgcgag gcgatgacgc gagagcgacc gcaccagaat
     2761 gagctcgatg cgctgatgaa caagcgggac gccgacctcg tggcggtcga gagcgagcgg
     2821 gcctcgcgtt tggcgcaagt cgatgacgac ctctcggccc aggtcgacga agtgatggaa
     2881 ca
//
LOCUS       DATMBB010000221         2645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_492760, whole genome shotgun sequence.
ACCESSION   DATMBB010000221 DATMBB010000000
VERSION     DATMBB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492760"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VNL92_04905"
     CDS             complement(<1..413)
                     /locus_tag="VNL92_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWO94083.1"
                     /translation="MFGLGVAKGMMITARRAFTRPFTVQYPEEQRPIPARARTNLVWF
                     VERCTGCSTCAQACPDGCILVETSQREDRSLNVDRYEIDFRLCMYCGLCVEACPYEAI
                     QAGGPLNDADYVFDNLYKNKETLTVLAQDYLREHD"
     gene            complement(424..1077)
                     /locus_tag="VNL92_04910"
     CDS             complement(424..1077)
                     /locus_tag="VNL92_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWO94084.1"
                     /translation="MSDQAAYLPVVPVSGIDEDVRSNILVSSVDTVLNWARRSSLWPA
                     MFGLACCAMEMIATATSRYDLARFGAEIFRASPRQADVMIVSGTVTWKMAPPVRRVYL
                     QMAEPKWVISMGSCANIGGPFAYGYSTLPGVNLIVPVDVYVPGCPPRPESLIAGLMLL
                     QEKIKQDSIAGERVRKEPSGDFSEYLPEGDPLRLELESLFTPEGISAVTSGAHAGEL"
     gene            1272..1922
                     /locus_tag="VNL92_04915"
     CDS             1272..1922
                     /locus_tag="VNL92_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWO94085.1"
                     /translation="MFRRPRRSEESIAAPADDAAQLQQDIAEEEDADLDEDGELPSDL
                     DEFLAEDAELEAEDAEPAVEDVTVATPVAAAAEPRPEVPATLAQLPEYEGEPPTIIGP
                     ATAIEGQVRTPDDLRVEGSVTGVVEVRGTLLIEPGGTVDAEVDARSIRARGRLHGKVH
                     CRDRLDIGEGSHVEGSFTTGILVIEEGAVVSGRFATSGGRMAEDRVTTGAEEARKR"
     gene            1919..2503
                     /locus_tag="VNL92_04920"
     CDS             1919..2503
                     /locus_tag="VNL92_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWO94086.1"
                     /translation="MTSTYTTDFDPATLLDQDQPFSRLDRFSRFDGRYEAMQNLLIEG
                     TCTGEIDCRGMLVVAEGARVNARIFARDMRIAGYIEGQFECSGRFELLPTARAQGAVK
                     ARQIVVHEGAEYDGELSMSDTVVGETEPAAWDTRPAPPATEEPATEEASAPSPPAAVE
                     PTLDATPEPASAASPETGEMPDFLKRRRSSRNSN"
BASE COUNT          492 a          876 c          878 g          399 t
ORIGIN      
        1 ttgtcatgct cgcgaaggta atcctgcgcc agcacggtca gcgtctcctt gttcttgtac
       61 aggttgtcga acacgtagtc agcgtcgttc agagggccgc cggcctggat ggcctcgtag
      121 gggcacgcct cgacgcaaag cccacaatac atgcacaggc ggaagtcgat ctcgtagcga
      181 tcgacgttca gcgatcggtc ctcccgttgc gatgtctcga cgaggatgca gccatccggg
      241 catgcctgcg cgcacgtgct gcatcccgtg cagcgctcca cgaaccaaac gaggttcgtc
      301 ctcgcacgcg cgggaatggg ccgctgctcc tccgggtact gcactgtgaa cgggcgtgtg
      361 aaggcgcgtc gcgccgtgat catcatgcct ttggcaacac cgaggccaaa catcgtgcct
      421 cctctagagc tcgccggcgt gcgcgccgct ggtaaccgcc gagattccct caggcgtgaa
      481 caacgattcg agctccaacc gcaacggatc gccctcaggc agatattccg agaagtcccc
      541 ggaaggctcc ttgcgcaccc gctcgcccgc gatcgagtcc tgcttgatct tctcctgcag
      601 caacatgagt ccggcgatga gcgattcggg ccgcggcggg caacccggga cgtacacgtc
      661 gactgggacg atgaggttca cgccgggcag cgtgctgtag ccataggcga acggcccgcc
      721 gatgttggcg cagctcccca tggagatgac ccacttcggc tcggccatct gcaggtaaac
      781 gcggcgaact ggaggcgcca tcttccacgt gacggtgccg gagacgatca tgacgtcggc
      841 ttgacgcgga gaggcacgga agatctcggc cccgaagcgc gcaaggtcat accgcgaagt
      901 cgccgtcgca atcatctcca tggcgcagca cgccagcccg aacatggcgg gccagaggga
      961 gctgcgccgc gcccagttga gcacagtatc gaccgacgaa acgaggatgt tgctccggac
     1021 gtcctcgtcg atgccgctca cggggacaac ggggagatag gcggcttggt cggacatgtg
     1081 gcgcctccat cggtccaccg cgctggacgt gccagcgcag ttcgtgccca gtgtaacaac
     1141 cgctcccgcg gcgcgacaag tcgcttgcgc aattcctaga gttttcctct tatgcttaca
     1201 cgtggtaacg tgcgcctagc ggccgaatcc gaatgcgcgc gcgagagcgc ttaggcacga
     1261 aggagggggc agtgttccgc cgaccacgcc gatcagagga gagcatagca gcgcctgcgg
     1321 acgatgcggc gcaacttcaa caggatatcg ccgaggaaga ggatgcggac ctcgacgagg
     1381 acggcgagct tcccagcgac ctcgacgaat tcctcgccga ggacgcggag ctcgaggccg
     1441 aggacgcaga gcccgccgtc gaggacgtta cggtggcgac gccggtcgcc gcggccgccg
     1501 agccgcgccc ggaagttccc gcaacgctgg cccagctccc cgagtacgag ggcgagccgc
     1561 cgacgatcat cggacccgcc acggcaatcg aaggccaggt ccgtacgccg gacgacctgc
     1621 gagtggaagg cagcgtcact ggggttgtgg aagtccgcgg cacactcctc atcgagcccg
     1681 gcggtaccgt cgacgccgag gtcgatgctc gctccattcg agcgcggggg cgattgcacg
     1741 gcaaagtgca ctgccgtgac cgtctggata tcggcgaggg tagccacgtg gaaggtagct
     1801 tcaccacggg catccttgta atagaggaag gcgccgtggt gagcggccgc ttcgccacca
     1861 gcggtggccg gatggctgag gatcgcgtga cgacgggagc cgaggaggcc aggaagcgat
     1921 gaccagcaca tacaccaccg acttcgaccc cgcgacgctc cttgaccagg accagccgtt
     1981 tagccgcctg gaccgcttct ctagattcga cggccgctac gaggcgatgc agaatctcct
     2041 catcgagggt acttgcacgg gcgagatcga ctgccgcggc atgctcgtcg tcgcggaggg
     2101 cgctcgcgtg aacgcccgca tcttcgcgcg cgacatgcga atcgcgggct acatcgaggg
     2161 tcagttcgag tgctcgggcc ggttcgagtt gcttcccacg gcccgggccc agggcgcggt
     2221 caaggcccgc cagatcgtcg tccatgaggg cgccgagtac gatggcgagc tgagcatgag
     2281 cgacaccgtc gtcggcgaga cggagcccgc ggcctgggac acgcgtccgg cgcccccggc
     2341 aaccgaggag cccgcaacgg aggaggcatc ggccccttcg ccccccgcgg cggtggaacc
     2401 gacgctcgac gcgacgcccg agccggcgag cgccgcctcg cccgagacgg gcgagatgcc
     2461 cgacttcctg aagcgcaggc ggagcagccg caacagcaac tagcgcatcg agccgccccc
     2521 aacgcgacgt cacccccgta ctcacagtga gggaaacgcc gccaccgctg ggctgcgttc
     2581 ggtgcgaggt caacctcgac aggtggcgtt gacagatcgt ttgggccacg tcatcatgcg
     2641 cccga
//
LOCUS       DATMBB010000222         3831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_477297, whole genome shotgun sequence.
ACCESSION   DATMBB010000222 DATMBB010000000
VERSION     DATMBB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477297"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VNL92_04925"
     CDS             <1..471
                     /locus_tag="VNL92_04925"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HWO94087.1"
                     /translation="GTLRLVRDVAEATGRLGVEPLAPEFTTPCLAALLDDRRAPVKSF
                     LLNQALIAGIGNIYADEALFAARIDPRRAAGSLSSEEVTRLHAAIREVLCAGIRNRGV
                     SFRDYRDAEGNAGGNQAYVRVFRRTGQPCDVCGTPIARIKVAGRSTHYCPQCQR"
     gene            complement(484..912)
                     /locus_tag="VNL92_04930"
     CDS             complement(484..912)
                     /locus_tag="VNL92_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWO94088.1"
                     /translation="MTRSILPESWALPLVDETNREFFTSGRLKVQKCAACGIVQHPPE
                     DVCHACGGMQFEYPEASGRGTVWSHTIVRHPVHPALADVVPYAVALVALEDHPHVRIV
                     GNVVNVPPEQVQVGLPVRVTFDAVRDEYGEVLLPQWEAMA"
     gene            complement(931..2091)
                     /locus_tag="VNL92_04935"
     CDS             complement(931..2091)
                     /locus_tag="VNL92_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWO94089.1"
                     /translation="MHGRVAVVGVGESQYYKRGGSPHSEFQLANIAIRNAVADAGLNI
                     EEVDGLVSYMDSRNEPVRLSAALGLRRLNFTAQTWGGGGNGTASAVAIADAALTAGYA
                     TNIVVFRALAQGQFGRFGQARGVERIGGEQAYTYPYGLVAPAHTCAMQTRRFMHEYGV
                     TQDALAAISLASYAHAQRNPRALRYGRPLTREEYDSSRWIAEPFHLFDCCPENDGAAA
                     AVLTTAERARSMRKKPAYIRAAAQGLNYRGGAAAFNSPDFPTAYYAGVAEQLWPRAGV
                     RPEDVDVAQFYENFTGPVVMAMSEMGFAPPDGIEEFVTNGNLEWPNGKLPLNTSGGNL
                     AEAYIHGFELIVEAVRQIQGESTCQVPDAKLSLTVAGPGFAPGSAILFSSEP"
     gene            complement(2166..3482)
                     /locus_tag="VNL92_04940"
     CDS             complement(2166..3482)
                     /locus_tag="VNL92_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWO94090.1"
                     /translation="MTAIHIDPPTGRLGFLTPGMGAVATTTYAGVMALRRGLTQPIGS
                     LTQMGHIRLGRRTAHRNPLIKDFVPLAELDDIVFGGWDPIPDNAHQAALRAGVLDEHD
                     LAAVRDEMEAIEPMPAVFDPDYVRRLTGTTNVKTGENKWDLTQQLMEDIQRFREDHKC
                     DRLVVVWCASTEAYEAAGPVHQSVEAFEAGLRRSDPAIAPSQMYAYAALQLGVPFANG
                     APNLTVDIPAMIELANRNNVPICGKDFKTGQTLLKTILAPGFKARLLGIQGWFSTNIL
                     GNRDGEVLDDPESFRSKEVSKLGVLETILQPDQYPELYGELYHKVRINYYPPRRDNKE
                     GWDNIDIFGWMGYPMQIKVDFLCRDSILAAPIVLDLALFMDLAKRAGQRGIQEWLSFY
                     FKSPQVAPGLYPENDLFIQQTKLKNTLRHWMGEDLITHLGTEYYEE"
     gene            complement(3580..>3831)
                     /locus_tag="VNL92_04945"
     CDS             complement(3580..>3831)
                     /locus_tag="VNL92_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94091.1"
                     /translation="AGVVGVPITADKTLIVACIVPAEPAPDLAAVRSHVERALAAYKR
                     PHEYRIVASLPRTANGKLQRRELRDRLLREMALDPTQAK"
BASE COUNT          642 a         1254 c         1243 g          692 t
ORIGIN      
        1 gggacgctcc gcctcgttcg cgacgtcgcg gaagcgacgg gccgcctcgg tgtggagcct
       61 ctcgctcccg agttcaccac gccatgcctc gcggccctcc tcgacgaccg gcgcgcgccg
      121 gtgaagtcgt ttctcctgaa ccaggcgctc atcgcgggca tcggaaacat ctacgccgat
      181 gaggcgctct tcgctgctcg catcgacccg cgccgcgccg cgggctcact ctcctcagag
      241 gaggtgacgc ggctccacgc ggcgattcgc gaggtgctct gtgccggaat ccgcaatcga
      301 ggcgtcagct tccgcgacta ccgcgatgct gaagggaacg cgggcggcaa ccaagcgtat
      361 gtgagagtct ttcgccgcac gggtcagccg tgcgacgtgt gcggcacccc gatcgctcgc
      421 atcaaggtgg cgggacgcag cacccactac tgcccccaat gccaacgata gtccgcccct
      481 gattcaggcc atcgcctccc actgaggcag aaggacttcg ccgtattcgt cgcggaccgc
      541 gtcgaacgtc acccgcacgg gcagacccac ctgcacctgc tcgggcggca cattcacgac
      601 gttcccgaca atgcgaacgt gcggatggtc ctcgagcgcc acgagggcca cggcgtaggg
      661 gactacatcg gccagcgccg ggtgcaccgg atgccggacg atcgtgtggc tccagaccgt
      721 cccgcggcca ctcgcctccg gatactcgaa ctgcatgcct ccgcaagcgt ggcatacatc
      781 ctcggggggg tgctgaacaa tgccgcaggc cgcgcacttc tgcaccttca gcctgccgct
      841 cgtgaagaac tcgcggttgg tctcgtcgac gaggggaagc gcccatgact cgggtaagat
      901 ggagcgtgtc atcgtttcga tctccttgct ctacggctcg gacgagaaga ggatggcgct
      961 gccgggcgcg aagccggggc cagccacggt gaggctgagc ttcgcatcgg gcacttggca
     1021 ggtcgactcg ccctgaatct gccgtaccgc ttcgacgatg agctcgaatc cgtgaatgta
     1081 ggcctcggcg aggttgccgc cgctcgtatt cagcggcaac ttcccgttcg gccactcaag
     1141 gttgccgttt gtaacgaatt cctcgatgcc gtccggaggc gcgaatccca tctcgctcat
     1201 ggccatgacg accgggccag tgaagttctc gtagaactgg gcgacgtcga catcttccgg
     1261 gcgcactccg gcacggggcc aaagctgctc ggcgactcca gcgtagtacg cagtcgggaa
     1321 atcgggactg ttgaaggcag cggcgccgcc ccggtagttg agcccttggg cggctgcccg
     1381 gatgtaggcc ggcttcttac gcatgctacg agcgcgctcg gctgtggtga gcaccgcggc
     1441 ggcagcgccg tcgttctccg ggcaacaatc gaagaggtgg aagggctccg ctatccagcg
     1501 ggagctgtcg tactcctccc tcgtcagcgg gcgcccgtac cgcaacgcac gcgggttgcg
     1561 ctgcgcatgt gcgtaagacg cgagcgagat tgccgcgaga gcatcctgcg tgacgccgta
     1621 ttcgtgcata aagcgtcgtg tctgcattgc gcacgtgtgg gcgggcgcga caaggccata
     1681 ggggtacgta tacgcttgct cgccaccgat gcgctcgact ccgcgggcct ggccaaaccg
     1741 gccgaattgg ccctgagcta gagcgcgaaa caccacgatg ttcgtggcgt agccggccgt
     1801 gagcgccgca tcggctatgg caacggccga tgcagtgcca ttcccgccgc caccccacgt
     1861 ttgggccgtg aagttgagtc gccgtaatcc cagcgcagcg ctcaatcgca ccggctcgtt
     1921 gcgcgagtcc atgtacgaga cgagcccatc gacctcctcg atgttcaggc ccgcatcggc
     1981 caccgcgttc cggatcgcga tgtttgccag ctggaactcg ctatgcggcg aaccgccgcg
     2041 cttgtagtac tggctctccc ccacgccgac cacggcaaca cgcccatgca tcgagcgcac
     2101 ctcctcacgg atggactcgc gcgcaacggt agccgcagcg ggccggacgt gtcaaacgga
     2161 ggccgctatt cctcgtagta ctccgtgccg aggtgcgtga tgagatcttc gcccatccag
     2221 tgacgcagcg tgttcttgag cttggtttgc tggatgaaca gatcgttctc cgggtagagg
     2281 ccgggggcga cctggggact cttgaagtag aagctcagcc actcctgaat gccgcgctgg
     2341 cccgcccgct tggccaggtc catgaacagc gcgaggtcga gcacgatcgg cgccgcgagg
     2401 atgctatcgc ggcacaggaa gtcgaccttg atctgcatcg gatagcccat ccacccgaag
     2461 atatcgatat tgtcccaccc ctccttgtta tcgcgccgcg gaggatagta gttgatgcgc
     2521 accttgtggt agagctcgcc gtagagctcc ggatactgat caggctggag gatcgtctcg
     2581 aggacgccga gtttgctgac ctccttactg cggaagctct ccggatcatc gaggacttcg
     2641 ccatcgcggt tgccgagaat gttcgtgctg aaccatccct ggatgccgag gagacgggcc
     2701 ttgaatccag gcgcaaggat cgtcttgagg agggtttgcc ctgttttgaa gtccttcccg
     2761 caaatgggga cgttgttccg attggcgagc tcgatcatag ctggaatgtc gacggtgagg
     2821 ttgggggcgc cgttcgcgaa cgggacaccg agctggagcg ccgcgtaggc gtacatctgg
     2881 ctcggtgcga tggcggggtc gctgcggcgc agcccggcct cgaacgcctc caccgactga
     2941 tgaacggggc ctgctgcctc gtaggcttcc gtgctcgcgc accaaacgac gacgagccgg
     3001 tcgcacttgt ggtcctcccg aaaccgctga atgtcctcca tgagctgctg ggtgaggtcc
     3061 cacttgtttt cgcccgtctt cacgtttgtc gtgccggtga gcctgcgcac atagtcggga
     3121 tcgaagaccg ccggcatggg ctcgatggct tccatctcgt cgcgcaccgc cgcgagatcg
     3181 tgctcgtcga gcacaccggc cctgagcgcg gcctgatgcg cgttatcggg aatcgggtcc
     3241 caaccaccga aaacaatgtc gtcgagctcg gcgaggggaa cgaagtcctt gatgagcggg
     3301 ttacggtgcg ccgtccgcct gcccagccgg atgtgaccca tctgcgtcag cgagccaatg
     3361 ggctgtgtga ggccacggcg cagcgccata acgccggcgt aggtcgtcgt cgccacggcg
     3421 cccatgcccg gcgtcagaaa gccgagacgg cccgtcggtg ggtcgatgtg aatcgcggtc
     3481 atgtcctgtg tctcctctcc tccgagcgag ggcggcgcaa gtcccagtct ccgcctccgg
     3541 tcaccgtaga gcctcgccgc cgatccgaca agggccttgt tacttcgcct gcgttggatc
     3601 gagcgccatc tcgcgaagca ggcgatcgcg cagttcgcgc cgctgcagtt tcccgttggc
     3661 cgtccggggc agcgaagcga cgatccggta ctcgtgcggg cgcttgtacg cggcgagcgc
     3721 cctctcgaca tgggagcgca cagccgcgag gtccggcgca ggctcggccg ggacgatgca
     3781 ggccacgata agcgttttgt cggcggtgat gggcacgccg acaacacccg c
//
LOCUS       DATMBB010000223         2999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_473072, whole genome shotgun sequence.
ACCESSION   DATMBB010000223 DATMBB010000000
VERSION     DATMBB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473072"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2963)
                     /gene="mfd"
                     /locus_tag="VNL92_04950"
     CDS             complement(<1..2963)
                     /gene="mfd"
                     /locus_tag="VNL92_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWO94092.1"
                     /translation="MRPDDMTSVEIVRGQKLPQDDLLRQLVTLGYSIEAITDRPGAAS
                     RRGGIVDVHAPDGDAPVRIEWLGDELESLREFDPTTQRSLRTIERAAFGPAGEGLLAF
                     TSEAIGSLRRSLNTASLNPEATERVESELSVLGSRGEADTFWLPFLLQATLFDHIPEN
                     ALLIVDERAECGSSLRDAEVAAALARDEREQLGELPGGLPLPFAAWDDVEAAIERQAR
                     RIELARWATGEGNGSIRLDFGQAPAFGGQLRSLVSGVAAEARSGDAVVVVSQQAPRIR
                     ELFQEVGIAAQAEITAPSAGSVAVASGIVSGGWSYQLEGRRLLLISDAETFGFRKQRR
                     LLPERRERVASTLLSEIEPGEYVVHIEHGIARFEGLVRRTSDGREREYMELRYAEGDR
                     LFVPIDQIARVSRYVGPSERPPSLTRLGGAEWSRTTARVRRAVMDLAKDLLDLYAARE
                     VAEGHAFSPDTPWQQELEASFPYVETPDQMDAIRATKADMERLTPMDRLVCGDVGYGK
                     TEVAVRAAFKAVMDGMQVAVLVPTTVLAQQHYETFSERCAGFPVRIEMLSRFRSEREQ
                     RRILAEMAEGSVDIVIGTHRLLQKDVAFKNLGLVIIDEEQRFGVMHKERLKRMRREVD
                     VLTLTATPIPRTLHMALAGIRDMSTIDTPPEARQPIRTYVAEYDERLVREAVRRELER
                     GGQVYFVHNRVRNIEHIAEKLRATVPEARIVVGHGQMAEGELERVMHVFAEGEADVLV
                     CTTIIESGIDIANVNTMVVNHADQLGLAQLYQLRGRIGRSARRAYAYLLYEKGKVLSE
                     VARKRLQAVFEANELSAGFRIAMRDLEIRGAGNLLGAEQSGHVGAVGFDLYTRMLAEA
                     VGRLKALRAGKPEPPPRPSTEVSIDLPLVAYLPESYVPELAVRLALYQRMADVRTPDE
                     AANLAREMRDRFGPLPPAAQQLLYAAGLRAAAMEAGVESIARSDDVLVLKLSQDTLDR
                     LSPGQRLGIERALPRG"
BASE COUNT          485 a         1009 c          998 g          507 t
ORIGIN      
        1 ccacgcggca gcgcgcgctc gatgccgagc cgttgccctg gcgacaggcg atcgagcgtg
       61 tcttgcgata gcttcagcac gagcacgtca tccgagcgcg cgatcgattc gacgccggct
      121 tccattgccg ccgcgcgcag cccggctgcg tacaggagct gctgcgccgc cggcgggagt
      181 ggcccgaagc ggtcgcgcat ctcgcgcgcc aggttggccg cctcatccgg cgtccgtacg
      241 tcggccatgc gctgatagag cgcgagacga accgcgagct ccggcacgta ggattcgggc
      301 aggtaggcga cgagcggaag gtcgatgctg acttccgtgg aaggccgcgg tggaggttcc
      361 ggcttgccgg cgcgcagggc cttgagccgc ccgacggcct cggccagcat ccgggtgtag
      421 agatcgaatc cgacggcgcc gacgtgtccg ctttgctctg cgccgagcag attgccagcg
      481 ccacgaatct cgagatcgcg catggcgatg cggaagcccg cactcagctc gttcgcctcg
      541 aacacggcct gcaatcgctt gcgggctacc tcgctcagca ccttgccctt ctcgtagaga
      601 aggtacgcgt aagctcggcg ggcgctgcgt ccaatacgcc cgcgaagctg gtagagctgt
      661 gccaggccca actggtcggc atggttgacg accatcgtgt tgacgttcgc gatgtcgatg
      721 ccggattcga tgatcgtcgt gcaaacgagg acatccgctt cgccctctgc gaagacgtgc
      781 atcacacgct cgagttcccc ctcggccatc tggccatgcc ccacgacgat acgggcttcg
      841 ggcacggtag cacgtagctt ttcggcaatg tgctcgatgt tgcgaacgcg gttgtgaacg
      901 aagtagacct gtccgccgcg ttccagctcg cgccgcaccg cctcccgcac gagacgctca
      961 tcgtactctg cgacgtaggt gcgaatcggt tggcgcgcct cgggaggcgt atcgatcgtc
     1021 gacatgtcgc gaatgccggc cagtgccatg tgcagggttc gcgggatagg tgtggcggtg
     1081 agcgtgagca catcgacctc acgccgcatg cgcttgaggc gctccttgtg catgacgccg
     1141 aaccgctgct cctcgtcgat gatgacgagg ccgaggttct tgaaggcaac gtccttctgc
     1201 aggaggcggt gagtgccgat gacgatgtcg acgctgccct cggccatctc ggcgaggatg
     1261 cggcgctgct ccctctcgga gcggaagcgc gatagcattt cgatgcggac ggggaagccc
     1321 gcgcaacgct ccgagaacgt ctcgtagtgc tgctgggcga gaaccgtggt cggaacgagg
     1381 acggccacct gcatgccgtc catcacggcc ttgaacgccg ctcgtacggc tacttccgtc
     1441 ttcccgtatc cgacatcgcc gcagaccaaa cggtccatcg gggtcaggcg ctccatgtcg
     1501 gccttcgttg cgcggatagc gtccatctgg tccggggtct ccacgtaggg gaagctcgcc
     1561 tccagttcct gctgccacgg cgtatcggga gaaaacgcat ggccctccgc gacctcgcgc
     1621 gccgcgtaga ggtcgagcaa gtccttcgcc aggtccatga cggcgcgccg cactcgggcc
     1681 gtcgtccgcg accactctgc gccaccgagc cgagtgaggg agggtgggcg ctcgctgggg
     1741 ccgacgtagc ggctcacccg cgcaatctga tcgatgggga cgaacagtcg atcgccctcc
     1801 gcatagcgga gctccatgta ctcgcgctcc ctgccgtcgg acgtgcggcg tacgaggcct
     1861 tcgaagcggg cgatgccgtg ctcgatgtgc acgacgtatt cgccgggctc gatctctgag
     1921 aggagggtgg aggcaacgcg ctcccggcgc tccggcagga gacgccgctg cttccgaaac
     1981 ccaaacgtct ccgcatcgct gatgaggagc agccggcgac cttcgagttg gtaggaccag
     2041 cccccggaga cgatgccgct cgccacggcg actgagccgg cgctcggtgc cgtgatctcg
     2101 gcctgggcgg cgattccgac ttcctggaac agctcccgga ttcgaggcgc ctgctgtgag
     2161 acgacaacga cggcgtcgcc cgagcgagct tccgccgcga cgcccgacac caacgaacgc
     2221 agctgcccac cgaacgccgg cgcctggccg aaatcgaggc gaatcgagcc gttcccttcg
     2281 cctgtcgccc agcgtgcgag ctcgattcgc cgggcctggc gttcgatggc ggcttctacg
     2341 tcgtcccatg cggcgaaggg gagggggagt cccccaggca gttcgcccag ctgctcgcgc
     2401 tcgtctctgg ccagggcggc ggcgacctcc gcgtcgcgca gggacgatcc gcactcggcc
     2461 cgctcgtcaa cgatgaggag cgcgttctcg ggaatgtggt cgaacagcgt cgcctgaagc
     2521 aggaacggga gccaaaaggt gtcggcttcc ccacgcgacc ccagcaccga cagctccgac
     2581 tccacgcgct cggttgcctc tggattcagc gaagcggtgt tgagcgaccg gcgcagcgag
     2641 ccaatggctt cgctggtgaa cgccaacagg ccttcacctg ccggtccgaa cgcagctcgc
     2701 tcgatcgtcc gaagcgaacg ttgcgtggtg ggatcgaact cgcgcaggct ctcgagctcg
     2761 tcgccaagcc attcgatgcg caccggcgca tcgccgtccg gcgcgtgcac atcgacgatg
     2821 ccaccccggc gggacgcggc gcccggtcga tcggtgatcg cctcgatgct gtagcccaac
     2881 gtgacgagtt ggcggaggag atcgtcctgc ggcagcttct gtccacgcac gatctctacg
     2941 ctcgtcatgt cgtcgggccg caacgtgcac tccgatgcgg cccgtatcga cgtgacgat
//
LOCUS       DATMBB010000224         2996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_1301, whole genome shotgun sequence.
ACCESSION   DATMBB010000224 DATMBB010000000
VERSION     DATMBB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VNL92_04955"
     CDS             <1..910
                     /locus_tag="VNL92_04955"
                     /inference="COORDINATES: protein motif:HMM:NF039222.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94093.1"
                     /translation="ATRGLRFSKVISFGNALDLNEADFLEYFAADPETELVAVYLEGV
                     QKGPRMFHALRSLAQRKPVVLLKGGATEAGSRAAASHTGSLAGAARVWQALARQSNAI
                     AVDSIEEVCDQLVALQALPHGLSGNRAAIIGSGGGTSVLAADACAREGIDVPALPGDV
                     QERLLEFTPLAGNSVRNPLDTFAITERDNFVRTVRIVGEAENIDFILLHTHAGWGFRQ
                     MTPGEALEQTIAGILASREATTKPVLVVLREPMSAEQAEPWMQLRTRCSAEGVPVFNG
                     IERAARALSRVVEWHEQRGELASQAG"
     gene            1037..1411
                     /locus_tag="VNL92_04960"
     CDS             1037..1411
                     /locus_tag="VNL92_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWO94094.1"
                     /translation="MVTENAVVSVTEKAAEKARALLDERGMPSGALRVFVAGGGCSGY
                     QYGMALATEAEEGDLVLEQHGVRLVIDPDSAPLLGGAEIDYVDDVMKSGFTIFNPNAV
                     KSCACGTSFQTKGGEGAPKSCC"
     gene            complement(1457..2140)
                     /locus_tag="VNL92_04965"
     CDS             complement(1457..2140)
                     /locus_tag="VNL92_04965"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HWO94095.1"
                     /translation="MSGAHPRRAVVAAGVPIVGTPIELDAMAEADLRVAADSGAETLL
                     RFGFTPHCLVGDLDSVDPHVVDRLRSAGVEVITYPERKDYTDTHLALRLARDRGAGDI
                     ALVGGAGGERLDHALANIAVLAGHEFRRVRMRLVSGYSEAHVVRSSLRIRGRRGEIVS
                     LLPVTSVVRGVSAQGFEYTLDGATLRRDASRGVSNRLVGEEGRIAVERGTLLVVQEHG
                     SEGPPSRTA"
     gene            2208..>2996
                     /locus_tag="VNL92_04970"
     CDS             2208..>2996
                     /locus_tag="VNL92_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94096.1"
                     /translation="MAHWLRYATPAPAAAPNYYPHIFPYRLPPLVEFDPEPVPLDPAP
                     AIWITDTTFRDGQQSRNPYSPQQIADLYQLLSRLGGPNGMIRQTEFFLYSRKDREAVE
                     RCLELDCRYPEITGWIRATPSDYAAVREMGLRETGILTSISDYHIYKKLRSDRESVLR
                     SYLEVVDLALADGVVPRCHLEDSTRADFHHVVLPFVQALMERSRESGIQVKIRFPDTL
                     GVGVPHPQAALPRGIPKLTYALRHEAGVPPEALEFHGQNDLNAIV"
BASE COUNT          527 a          994 c          971 g          504 t
ORIGIN      
        1 ggctacgcgc ggccttcgct tcagcaaggt catcagcttc ggcaatgccc ttgacctgaa
       61 cgaagcggac ttcctggaat acttcgcggc cgaccccgag accgaacttg tcgccgtgta
      121 cctcgagggt gtgcagaagg gcccgcgcat gttccatgct ctgcggtcgc ttgcgcaacg
      181 gaagcccgtc gtcctgctca agggcggggc aacggaggcg gggagccgag cggcggcgtc
      241 gcatacgggg tcgctcgccg gggcggcgcg cgtgtggcag gccctcgcgc ggcaatccaa
      301 cgccatcgcc gtggattcga ttgaggaggt ctgcgatcaa ctcgtggcgt tgcaggcgct
      361 gccgcacggg ctatcgggca atcgagcggc catcattggc tcgggcgggg gcacaagcgt
      421 gctcgccgct gatgcgtgcg cgcgggaggg catcgacgtg cccgccctcc ctggcgacgt
      481 tcaggagcgg ctgctcgagt tcacgccttt ggcaggtaac agcgttcgca acccgctcga
      541 caccttcgcc atcaccgagc gggataactt cgtacgcacc gtgcgcatcg ttggtgaggc
      601 cgagaacatc gacttcatcc ttctccatac tcatgcgggt tggggctttc gccagatgac
      661 gcccggggag gcactcgagc agaccatcgc cggcatcctc gcctctcgag aggcaacgac
      721 caagcccgtg ctcgtggtcc tgcgcgagcc gatgagcgcc gagcaggccg agccctggat
      781 gcaactccga acgcgttgct cggccgaggg agtgccggtg ttcaacggaa tcgaacgggc
      841 ggcgcgcgcg ctaagccggg tagtggaatg gcacgagcag cgaggcgagc tcgcctcaca
      901 ggcgggctga caggcggggc gcagcccgat gtttgacagg cagccggcga ctttctacac
      961 ttgatagcgt cgtaaaggaa gaggttcgcc gtgccgcggt gccgtgccgg cgatttgaag
     1021 gcgagggagc aaacctatgg taaccgaaaa cgccgtcgtc agcgtgacgg aaaaggcagc
     1081 agaaaaggct cgcgcgcttc tcgatgagcg cggcatgccg agcggcgcct tgcgcgtctt
     1141 cgttgctggt ggaggctgct ccggttacca gtacggcatg gcccttgcca cagaggccga
     1201 agagggcgac ctcgtgctcg agcagcacgg cgtccgtctt gtcatcgacc cggacagcgc
     1261 gcccctgctc ggaggcgccg agatcgatta cgtcgacgac gtgatgaaga gcggcttcac
     1321 catctttaac ccgaacgccg taaagagctg cgcctgtggc accagcttcc agaccaaggg
     1381 cggcgagggc gcgccgaaga gctgctgcta acgaactgag ccgcgcgtat gcatgaagcc
     1441 ccggacagcc ggggcttcat gctgtccggc tcggcgggcc ttcggacccg tgctcctgaa
     1501 cgacgagtag cgtcccccgc tcaacggcga tccgtccctc ctcgcccacc aagcggttgc
     1561 tgacgccgcg ggaagcatcg cgccgcagtg ttgcgccgtc cagcgtgtac tcgaagccct
     1621 gcgccgatac gccccgcacc acgctcgtca cgggcagcaa cgaaacgatc tcgcctcggc
     1681 gcccccgaat tcgcagcgat gaccggacga cgtgcgcttc gctgtatccc gaaacgaggc
     1741 gcatgcggac tcggcggaac tcgtgcccag ccaggacagc gatattcgcg agggcatggt
     1801 ccagtcgctc gccgccggct ccgccgacca gcgcgatgtc cccggcccca cggtcgcgcg
     1861 cgagacgcaa cgccaggtgc gtgtccgtgt agtccttccg ctccgggtag gtgatcacct
     1921 ccacgcccgc cgacctgagg cgatccacaa cgtgcgggtc cacggagtcg agatcaccga
     1981 cgagacagtg gggcgtaaag ccaaagcgca gcagcgtctc ggcgccggaa tccgcggcga
     2041 cgcggagatc tgcctccgcc atcgcgtcga gctcgatggg cgttccgacg atcggaacgc
     2101 cggcggcaac gaccgcgcgg cgtggatggg cgccgctcac gggcattgac tcggtgcgga
     2161 ttgcatacta tatacaatca tgtaacgacc gcagaatgga ggcggccatg gctcattggc
     2221 tgcgttacgc tactccggcg cccgcggcgg ctcccaacta ctacccccac attttccctt
     2281 atcgcctgcc accgctcgtc gagttcgatc cggagcccgt tccgctcgat cccgcccctg
     2341 cgatatggat tacggacacg actttccgtg atgggcagca gtcgcgcaat ccgtattcac
     2401 cccagcaaat cgccgatctg taccagctgc tatcccggct cggcggtccg aacgggatga
     2461 tccgccaaac ggagttcttc ctctactctc ggaaggaccg cgaggcggtc gaacgctgtc
     2521 tggagctcga ctgccgttat cccgagatca ccggctggat tcgcgctaca ccgagcgatt
     2581 atgcagcggt ccgggagatg ggcctccggg aaaccgggat actgacatcg atctccgatt
     2641 accacatcta caagaagctg cgtagcgacc gcgaatcggt cctgcgttcc tacctcgaag
     2701 ttgtcgacct cgccctcgcc gatggtgtcg tgccgcgctg ccacctggaa gatagcacgc
     2761 gggcggattt ccaccacgtc gtgctgccgt tcgtccaggc gctgatggag cggtctcgtg
     2821 agtcgggcat acaggtgaag attcgctttc ccgatacgct cggggtgggc gtgccgcatc
     2881 cgcaagcggc tctccctcgg gggataccga agctcaccta cgctctgcgc cacgaggcgg
     2941 gtgtgccgcc cgaggccctc gagtttcacg gccagaacga cctgaatgcc atcgtc
//
LOCUS       DATMBB010000225         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_6149, whole genome shotgun sequence.
ACCESSION   DATMBB010000225 DATMBB010000000
VERSION     DATMBB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6149"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VNL92_04975"
     CDS             <1..310
                     /locus_tag="VNL92_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO94097.1"
                     /translation="IGTEERIVGIARYDRSPDTKQAEVAVLVHDAYQQRGLGTAMLTT
                     LADIARENGIETLEADVLPYNQRMLEVLLHLGYRLEAGSAKRTGLVRLLKPLKPATES
                     "
     gene            complement(307..2037)
                     /gene="polX"
                     /locus_tag="VNL92_04980"
     CDS             complement(307..2037)
                     /gene="polX"
                     /locus_tag="VNL92_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWO94098.1"
                     /translation="MRNREVAAILDEVADLMELLGEDRYRVARYRDAAMKIEHFPEPI
                     ENLEREGRVQAIRGVGKSIGGKVEEYLRSGSLRQLEELRARVPPAALTLMRIPGVGPK
                     RALLLTERLGVRSVDDLAQAIERGDVARLPRMGPKVAEQLLEEIHRLRTRSKRIPLGV
                     AAALADDVVRLLSECKAAQRIEAAGSLRRMQETIGDIDILVASAEPSQVIDAFVRMAI
                     VRDVLAAGDTRASVLTQNDLQVDVRVVPGESWGAALQYFTGSKEHNVRLRELAVRRGW
                     RLSEYGLFDGEQRIAGSTEAEVYEALGLAWVPPELRENQGEIEAALAHQLPDLIGYGD
                     LRGDLHAHTRLSDGSSTIEQMAEAAAARGYEYLAITDHSQALGVARGMTEDELRAEHA
                     AIRRAQAMVPTTRLLCGVEVDIRVDSRLDCSDDFLAECDVVVASIHSAFQRSAADQTA
                     RLIAAIEHPCVDIVGHPTGRMLGKRRGYDIDLAAVLDAAARTGTALEVNAQPNRLDLP
                     ADAIRAAVERGVMLAINTDAHEAQQLGQTRLGIGTARRGWVPSDLVLNALPLEGLLAW
                     LRERRSRAAA"
     gene            2103..>2243
                     /locus_tag="VNL92_04985"
     CDS             2103..>2243
                     /locus_tag="VNL92_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWO94099.1"
                     /translation="MPSTISLAEGQTASFEKTITEDDVRAFGNASGDMNPLHFDDAYA
                     RRT"
BASE COUNT          381 a          776 c          720 g          366 t
ORIGIN      
        1 cataggcacc gaagagcgga ttgtcggcat cgcccggtat gatcggtctc ccgacacgaa
       61 gcaagcagag gttgccgtcc tcgtccacga tgcatatcag cagcgggggc tggggacggc
      121 catgctgacg acgctcgccg atatcgcgcg ggagaatggc atcgaaacgc tcgaggccga
      181 cgtgctgccc tacaaccaac ggatgctgga agtgctgctg cacctcggct accggctgga
      241 ggccggatcg gcgaaacgga cgggtctggt ccggctactc aaacctctaa agccggcaac
      301 ggaatcctag gccgcggcac gcgaccggcg ctcccgcagc caggcgagca agccttccaa
      361 cggcaaggca ttcagcacta agtccgacgg cacccaaccg cgccgcgccg tcccgatacc
      421 cagcctcgtt tgtcccagct gctgcgcctc gtgtgcgtcg gtgttgatgg ccagcatgac
      481 gccacgttcc accgcggctc gtatggcatc ggcgggaaga tcgaggcgat tgggttgtgc
      541 gttcacctcg agcgcggttc ccgtgcgagc agcggcatcg agcaccgccg ccaggtcgat
      601 gtcgtagccg cgacgtttgc cgagcatgcg cccggtcgga tgcccgacga tgtcgacgca
      661 cggatgctcg atggcggcga tgaggcgtgc cgtctggtcg gcggcacttc gctggaacgc
      721 ggagtgaatc gaggccacca cgacatcgca ctctgcgagg aagtcgtcgc tgcaatccag
      781 gcgggaatcg acccggatgt cgacctcgac gccacacaga agtcgcgtcg tcgggaccat
      841 cgcctgtgcg cgacggatcg ctgcgtgctc ggcacggagc tcgtcctccg tcatgccccg
      901 cgccacgccg agcgcctggc tgtggtcggt gatggcgagg tattcgtagc cacgcgctgc
      961 tgccgcttcg gccatctgct cgatcgtgct ggagccgtcg ctcaagcgag tgtgggcgtg
     1021 cagatcgccg cgtagatcgc cgtagccgat gaggtcgggc aactggtgcg cgagcgccgc
     1081 ctcgatctcc ccctggttct cacgcagctc gggcgggacc cacgcgaggc ccagcgcctc
     1141 gtagacctcg gcctcggtgc tccccgctat gcgctgttcg ccatcgaaca agccgtattc
     1201 gctcaggcgc cagccccgcc gcaccgccag ctcgcgcagt cggacgttgt gctccttcga
     1261 gcccgtgaag tattgcagcg ccgcacccca cgactcgcct ggaacgacgc gcacgtcgac
     1321 ctgcagatcg ttttgcgtga gaacggacgc gcgcgtgtca ccagccgcca gcacgtcgcg
     1381 aacgatggcc attcgtacga aggcgtcgat cacctgtgag ggttcggcag aggccacgag
     1441 aatatcgata tcgccgatcg tttcctgcat gcggcgcagg ctaccggccg cctcgatgcg
     1501 ctgagccgcc ttgcactcgc tgagaagacg cacgacgtca tcggcgaggg cggccgctac
     1561 gccgagcggt atccgcttcg atcgggttcg caaccggtgg atctcttcca gcagctgctc
     1621 tgccaccttg ggccccatcc tcggcagcct cgcgacgtct ccccgctcga tcgcctgggc
     1681 gaggtcgtcg actgaacgga cgccgagccg ctccgtgagg agcaaggcgc gcttcggccc
     1741 cacgccggga atacgcatca gcgtcagcgc tgccgggggc acgcgggccc gcaactcctc
     1801 gagctggcga agcgagccgc tgcgtaggta ttcctcgacc ttgccgccga tgctcttccc
     1861 gacgccccgg atcgcctgca cgcggccctc acgctcgagg ttctcgatcg gctccgggaa
     1921 gtgctcgatc ttcatcgcgg cgtcccgata gcgcgccacg cgataccggt cctccccgag
     1981 gagttccatc aggtccgcca cctcgtcgag gatcgccgcc acctctcggt tccgcatagg
     2041 gttatggtac agtcggtgac cagcggtggg cagagccgca agtaatacgc cggaggaact
     2101 cgatgcccag caccatctcg ctcgccgagg gccaaaccgc gagcttcgaa aagacgatca
     2161 ccgaggacga cgttcgtgca ttcgggaacg cgagcggcga catgaaccca ctccacttcg
     2221 acgacgcgta cgcaaggcgc acg
//
LOCUS       DATMBB010000226         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_402501, whole genome shotgun sequence.
ACCESSION   DATMBB010000226 DATMBB010000000
VERSION     DATMBB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402501"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..865
                     /locus_tag="VNL92_04990"
     CDS             5..865
                     /locus_tag="VNL92_04990"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWO94100.1"
                     /translation="MARRRSDLSRMAVQRLIREGNVLVNGHAAKASLRLEAGDRVALY
                     VPPPRPVLLEAEALPLVVRYEDDDLLVIDKPAGIAVHPAPGHERGTLVNALLHLVPDL
                     AGVGGELRPGIVHRLDKDTSGLIIVAKHDAAHRSLAAQMKAREVAKTYLAIVEGHPVP
                     PEARIDAPIGRHARDRKRMAIVAGGREARTTYRVLESFTEAALLEIGLETGRTHQIRV
                     HLASVGHPVLGDRLYGRPSPLIGRQALHASRLRFRSPSTGREIDVRCDMPVDMRDALA
                     ALRGVSLATR"
     gene            complement(855..1349)
                     /locus_tag="VNL92_04995"
     CDS             complement(855..1349)
                     /locus_tag="VNL92_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWO94101.1"
                     /translation="MIRASRLDVTLYEEVEADRNLTVEALGVVLLGSLAYGLGVGLAS
                     EPIALVFGLAGALLSWFAYAGIAYFVGTRVLAEQTTQADWGQLLRAVGYANAPNVLLV
                     TLFIPILGFLVYIGVSVWVLIATVLAVRQALDYRSTGRAIATSAIAWVVQIVIFSVLF
                     AVIV"
     gene            1488..>2139
                     /gene="gltX"
                     /locus_tag="VNL92_05000"
     CDS             1488..>2139
                     /gene="gltX"
                     /locus_tag="VNL92_05000"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWO94102.1"
                     /translation="MAVRVRYAPSPTGEPHVGNIRTALFNWLFARRHSGAFIVRIEDT
                     DRARLQEGALEAILDALHWLGLDWDEGPGVGGPDAPYVQSQRLALYASAAQRLVDAGH
                     AYECTCTPERLDEMRRAQQARKEPPRYDGRCRRRERSEIEADKAAGLPAVVRFAMPLE
                     GETTVHDLIRGDVSFENALLDDFILLKSDGFPTYHLAHIVDDHEMRISHVLRAEEWL"
BASE COUNT          366 a          730 c          713 g          330 t
ORIGIN      
        1 cttcattgcc cgccggcgga gcgatctctc ccggatggct gtgcagcgcc tcatccggga
       61 gggcaacgtg ctcgtcaacg gtcatgctgc gaaggccagt ctccggctcg aagctggcga
      121 tcgcgttgcc ctgtacgtac cgccgccgcg cccggtactc ctcgaagcgg aggcgctccc
      181 cctcgtcgtt cggtacgagg acgatgacct gctggtcatc gacaagccgg ccggcatcgc
      241 cgtccacccc gcgcccgggc acgagagggg tacgctcgtc aatgccctgc tgcacctcgt
      301 gcccgacctc gctggcgtcg gcggcgagct tcggccgggc atcgtgcatc ggctggacaa
      361 ggacacgtcg gggctcatca tcgtcgcgaa gcacgatgcg gcccatcgga gcctcgccgc
      421 gcagatgaag gcgcgcgaag tcgccaagac gtatctggca atcgtggagg ggcatcctgt
      481 tccgccagag gcgcgcatcg atgcgcccat cgggcgtcac gcccgcgacc gcaagcggat
      541 ggccatcgtc gccggcgggc gtgaagcccg cacgacgtac cgcgtgctcg agtcgttcac
      601 cgaggcggcg ctcctggaga tcggcctgga gacgggccgc acgcatcaga tccgggtgca
      661 ccttgcctca gtcggccatc ctgtcctcgg cgaccggctc tacggccggc cttcaccgct
      721 catcggcagg caggccctgc acgcgagcag gctccggttc cgttcgccat cgacgggccg
      781 cgagatcgac gtgcggtgcg atatgcctgt ggacatgcga gatgcgctgg ccgcgctgcg
      841 gggcgtgagc cttgctacac gatgacggcg aacagcacgc tgaagattac gatctgtacg
      901 acccaggcga tggccgatgt cgcgatggca cggcccgtgc tccgatagtc gagcgcctga
      961 cggaccgcga gaacggtggc gatgagcacc cacacggaca cgccgatata gacgaggaac
     1021 ccgagtatgg gaatgaacaa cgtcaccagg aggacgttcg gagcgttcgc gtatccgacg
     1081 gctcggagca gctgccccca gtcggcctga gtcgtctgtt cggccaggac tcgcgtcccc
     1141 acgaagtacg caatgcccgc ataggcgaac caggagagca gcgccccggc gagaccaaac
     1201 acgagcgcaa tcggctcgga cgccaggcca acgccgaggc catacgccag ggagccgaga
     1261 agcacgacgc ccagcgcttc caccgtgagg ttgcggtcgg cttcgacttc ctcgtacagc
     1321 gtgacgtcga ggcgcgaggc gcgaatcatc cgatcgatca ttgggcaccc cttggtcctg
     1381 gccgcgttcc cgctacccta acacgagtcc ctcggatggc cacgtccctt tcggtaggac
     1441 atgccgatcc gctgatacaa tagccgcaat ctgccgcggg agaaaccatg gccgttcgcg
     1501 tccgttatgc gcccagcccc acgggcgagc cgcatgtcgg caacatccgc acggcgctct
     1561 tcaactggct gttcgcccgg cggcatagcg gcgcgttcat cgttcgcatc gaagacacgg
     1621 accgcgcgcg tctgcaggag ggcgcgctgg aggcgattct cgatgcgctg cactggctgg
     1681 gcctcgactg ggacgagggg ccgggtgtcg gcgggccgga cgccccgtac gtccagtcgc
     1741 agcggctcgc gctctatgcc tccgccgcgc agaggctcgt cgatgccggc cacgcgtatg
     1801 agtgcacgtg tacgcccgag cggctggacg agatgcggcg ggcgcagcaa gcgcgaaagg
     1861 agccgcctcg ctacgatggc cgatgccggc gccgcgagcg cagcgaaatc gaggcggaca
     1921 aggcggcagg cctcccggcg gtggtgcgat tcgcgatgcc cctcgagggc gaaaccacgg
     1981 tgcacgacct catccggggc gatgtcagct tcgagaacgc gctcctcgac gacttcattc
     2041 tccttaagtc ggacggattc cccacgtatc atctcgccca catcgtcgat gatcacgaga
     2101 tgcgcatctc ccatgtgctg cgagcggagg agtggctgc
//
LOCUS       DATMBB010000227         3914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_358370, whole genome shotgun sequence.
ACCESSION   DATMBB010000227 DATMBB010000000
VERSION     DATMBB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358370"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1482
                     /locus_tag="VNL92_05005"
     CDS             100..1482
                     /locus_tag="VNL92_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWO94103.1"
                     /translation="MTPVALLTMLAFIAVSGGLAYWQILRASDLNERGDNPRVVQQAH
                     REDRGRILDRNVEVLAYTDTSTEQVRLYARPSLAQVIGYSSLRFGQNGIELAEHEYLS
                     GERSTDVFEFLLNELLGRERAGDDVVLTIDGALQDVAVDAMAGRRGAAIALDPRTGEV
                     LASVSNPGYDPNAIEDTGEALRNDPSQPLLNRATQGLYPPGSTFKTVTAAAALDTGRY
                     EADTPFSCHDALVFEGFEVECANVPEGEGDYDWGFGYVHSINAVFAQTGVDIGWPTLR
                     EFARRFGFGTDLGIETESSVSRVGGDDDSLASLASTAFGQGELLVTPLQMALVAATVA
                     NEGVVPQPHFVLRVQDRDGDVVDRRNPGSRGRAMSEATAQTLTELMVRNVEEAAGSLA
                     AIPGVAVAGKTGTAETGRPDEEPHAWFIGFAPAEEPVIAVAVVLENAGFGGTYAAPVA
                     GQIMAAAVGR"
     gene            1505..2302
                     /locus_tag="VNL92_05010"
     CDS             1505..2302
                     /locus_tag="VNL92_05010"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWO94104.1"
                     /translation="MNPLQRLETALREAFERPVWALGGQRLHPARLGDLLEDAMEQGR
                     LSLASGTFVPERYALLVHPDDYRRFRALVGAVEQGLAEHLEAVRQAQHLQCRRPITVQ
                     LRPDEGVRRGEAEVDAAFLERGGTSPAIARTPQGSAPPLGATMELDRRAVLAEATRIA
                     STMTEPILGKLEALDQQGQTVGAEYGIAVLPCTIGRSPECDIVLEDLRVSRRHARLTR
                     DGEAFIIEDLGSANGTWLDGARIEQAHVHDGATLTLGGPRFRLRLWS"
     gene            2307..2801
                     /locus_tag="VNL92_05015"
     CDS             2307..2801
                     /locus_tag="VNL92_05015"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWO94105.1"
                     /translation="MEDNLLIIIRLTLLAILYLFLWAVLSAVWRDIRRAPQRAPATYA
                     LAGMPRLYVVEPGSTTLRQGEVIDLQPLTTLGRSAVNTLTLQDETVSARHAAVEYRDG
                     RWWVHDLDSTNGTYVNRRRLAGTTELQPQDLLHIGRIAFQFAVETPQVYAGAARRPTA
                     RISR"
     gene            complement(2756..>3914)
                     /locus_tag="VNL92_05020"
     CDS             complement(2756..>3914)
                     /locus_tag="VNL92_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWO94106.1"
                     /translation="ADQLLLPLAAMLSAIGLVFVLRLRPDLAMDQLTWLVFGGALMAI
                     ALPLLSRYQMLAQYQYVAAMAGIGLLAITALFGTEINGARLWLSVGGFSFQSTELLKV
                     LLIVFLAAYLAERSQLLSGASLQGWRMQGRVRMRMPTLPYLIPLAIIWGMTLLMLIWQ
                     KDVGAVAILVGVTLGMLYVATGRLTFVLVGAVLLVVNIALTYRLFGYVQERVDTWLDP
                     WAETDAAGYQIVQSLYAFASGGFTGKGLGEGSPEYIPAVHTDFVFAALGEELGLLGAF
                     GVLVIYLLFVFRGYRAAMVQSSAFGQLLALGAAGVIALQAIVIMAGNLALIPVTGVTL
                     PFISYGGSSIVVNFVLLALLVRLSAPPSNEEERGTATQREMRAVGRRAAPA"
BASE COUNT          725 a         1347 c         1234 g          608 t
ORIGIN      
        1 cgggtaagct ccgccgtcgc cacccggcac tcgttcgctc catcattcgc ccttgcctat
       61 actcgatcta tgcgccggac gaaccctatc gcgggccgca tgacgcccgt cgccctactg
      121 acgatgctcg ccttcatcgc cgtttccggg ggtctcgcct attggcagat cctgcgcgcc
      181 tccgacctca acgagcgggg cgataatcct cgcgtcgtcc agcaggcgca tcgcgaggac
      241 cggggccgca ttctcgatcg caacgtcgag gttctcgcct acaccgacac gtcgaccgag
      301 caggtgcgcc tctacgcgcg tccatccttg gctcaggtga tcggctactc gagtctgagg
      361 ttcggccaaa acggcatcga actcgcggag catgagtacc tgagcggtga acgctcgacc
      421 gacgtgttcg aattcctctt gaacgagctg ctggggcgcg aacgtgcagg agatgacgtg
      481 gtactcacca tcgacggcgc ccttcaggac gtcgccgtcg atgcgatggc ggggcgtcgt
      541 ggcgccgcta tcgccctcga cccgcgaacg ggagaagtgc tggccagcgt gagcaacccg
      601 ggatacgacc cgaacgccat cgaggatacg ggtgaagcct tgcgcaatga tccatcgcag
      661 cccctcctga accgcgccac acagggcctc tacccacccg gctctacctt caagacggtg
      721 acggccgccg ccgccctcga taccggccgc tacgaagcgg acacgccatt cagctgccac
      781 gatgcgctcg tgttcgaggg gttcgaggtg gagtgcgcca acgtccccga gggcgaaggg
      841 gattacgact gggggttcgg atacgtccac tcgatcaacg ccgtgttcgc gcagacaggc
      901 gtcgatatcg gctggccgac actccgcgag ttcgcccggc ggttcgggtt cggcaccgac
      961 ttgggcatcg agaccgagtc gagcgtcagc cgtgtcggcg gcgatgacga ttcgctcgcc
     1021 tcgctggcga gcaccgcgtt cgggcagggc gagctgctcg tgaccccgct gcagatggcg
     1081 cttgtcgccg cgaccgtcgc caacgagggg gtcgtgccgc agccgcactt cgtgctgcgc
     1141 gtgcaggatc gcgacggcga tgtcgtggac cgtcgcaacc ccggatcgcg aggccgagcc
     1201 atgagcgaag cgacggcaca gacgttgacg gagctgatgg tccgaaacgt ggaagaggcg
     1261 gccggatcgc tggcagccat accgggcgtg gccgttgcag gcaagaccgg aaccgctgag
     1321 accggccgcc ccgacgagga accccacgcc tggttcatcg ggtttgcgcc ggccgaggag
     1381 ccggtcatag cggttgccgt cgtcctcgag aacgccggct tcggcggtac gtacgccgcg
     1441 cccgtggccg gacagatcat ggccgcggcg gtcggccgct gagtctcaag ggtatacttt
     1501 cgccatgaat ccactccagc gtctcgagac cgcgctccgc gaagcgttcg aacggccggt
     1561 ttgggccctt ggcggccagc gactccaccc ggcgcggctc ggcgaccttc tggaggatgc
     1621 gatggaacaa ggccggctct cgctcgcttc gggcaccttc gtcccggagc gatacgcgct
     1681 cctcgttcac ccggacgatt accggcgctt ccgcgctctc gtgggtgcag tcgaacaggg
     1741 cctcgcggaa catctggagg ccgtgcgcca ggctcagcat ctccagtgcc gtaggcccat
     1801 cacggtccag ctccgccccg acgagggcgt ccgccgcggc gaggcagagg ttgacgccgc
     1861 gttccttgaa cgaggcggca cctcgcccgc aatcgctcgg actccccaag gttcggcgcc
     1921 gcccctaggt gcgacgatgg agctcgatcg acgggctgtc ctcgccgagg cgacccgcat
     1981 cgcgtccacg atgacagaac cgatcctcgg gaagctggag gctctcgatc agcaaggcca
     2041 gacagtcggc gcagagtatg gaattgccgt cctgccttgc acgataggcc gctcacccga
     2101 atgcgatatc gtcctcgagg acctgcgcgt gtcgcgacgg cacgcccggc tcacccgcga
     2161 tggcgaggcg ttcatcatcg aggacttggg cagcgcgaac ggcacgtggc tcgacggcgc
     2221 ccgcatcgag caggcgcacg tgcacgatgg agcgacactc acgctcggcg gcccgcgctt
     2281 tcgactgagg ttgtggagct gaggcggtgg aggacaacct tctcatcatc atccgcctca
     2341 cgctcctcgc cattctctat ctcttcctgt gggcggttct cagcgccgtc tggcgcgaca
     2401 tccggcgggc tccgcagcgc gcacccgcca cgtacgcgct ggctgggatg ccgcggctct
     2461 acgtggtcga acccggatcc accactctgc gccagggtga ggtcatcgac ctgcagccac
     2521 tgaccaccct tggccgcagt gctgtcaata ccctaacgtt gcaggacgaa accgtgtccg
     2581 cccgccacgc cgcggtcgaa tatcgcgatg ggcgctggtg ggtgcacgac ctcgattcga
     2641 cgaacggaac ctacgtcaac cgccgaaggc tcgccgggac gacagagctc caaccgcagg
     2701 accttctgca tatcggccgt atcgcgtttc aattcgcggt cgaaacgccg caagtctatg
     2761 ctggcgccgc gcggcggcca accgcgcgca tttcgcgttg agtcgcggtc ccgcgctctt
     2821 cctcgttgct tggcggcgcc gacagacgta cgagcaacgc cagcagcacg aagttcacga
     2881 caatcgacga gcccccgtag ctgatgaacg gcaacgtcac tccggtgacc ggaatgaggg
     2941 cgaggtttcc tgccatgatc acaatggcct gcagcgctat gactcccgct gcgccaaggg
     3001 cgagaagctg cccgaacgcg ctggactgaa ccatggcagc ccgatagcca cgaaacacga
     3061 acagtagata gataaccagc accccaaacg cccccaggag ccctagctcc tcaccaagcg
     3121 ccgcgaacac gaagtcggtg tgaaccgcgg gaatgtactc cggcgaaccc tcccccaggc
     3181 ccttgcccgt gaatccccct gaggcgaacg catagagaga ctgcacgatc tgatagcccg
     3241 ccgcgtccgt ctccgcccag ggatcgagcc acgtatcgac acgctcctgg acgtaaccga
     3301 acagccgata cgtgagggcg atgttgacta ccagaagcac ggccccaacg agaacgaacg
     3361 taagccgccc cgtagcgacg tacagcatcc cgagcgtcac acccaccagg atggcgacgg
     3421 cccccacgtc cttctgccag atgagcatga gcagcgtcat gccccagatg atggcgagtg
     3481 ggatgaggta gggcagggtg ggcatccgca tccggacccg gccttgcatt cgccagccct
     3541 gcaacgatgc gccggacaac agctgcgaac gttccgccag atacgccgcg aggaacacga
     3601 tgagaagcac cttcagcaac tcggtgctct ggaaggaaaa ccccccaacg gagagccaca
     3661 gacgcgcacc gttgatctcc gtcccgaaca gcgcggtgat ggccaggagg ccaatgcccg
     3721 ccatggcggc gacgtactgg tactgcgcca acatctgata gcgactcagc agcggcagcg
     3781 cgatcgccat cagcgccccg ccgaacacga gccacgtcag ctggtccatc gccaagtcgg
     3841 gacgcagccg caggacgaag acgagcccga tggcggaaag catggccgcc agcggcagca
     3901 gaagctgatc ggct
//
LOCUS       DATMBB010000228         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_59128, whole genome shotgun sequence.
ACCESSION   DATMBB010000228 DATMBB010000000
VERSION     DATMBB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59128"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VNL92_05025"
     CDS             complement(<1..1560)
                     /locus_tag="VNL92_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94107.1"
                     /translation="MSYWFDPSAPLPSDNAASPMRGVYRRILGYMALFWGGIATAVAL
                     AMASSLFVVLQPWPIKFAIDGVLVSSQLDLGPLGEFPTASDGEKLRAAAALAGAYFGV
                     TVAGVVLGALSFYWIARTALYMIHGLRSRLMAHLRRLSLRFHAQQSLGDTIWRAINDA
                     RSVQEVMIFGVRTWSILILRLALMVALMAVLDWRLTLVALAALPLLFLTIHVLTGRIQ
                     STSQESREHMARMTSLIEQTMGSIRAVQVFGREGPERQRFERTSLAFVQAQLRFRVAE
                     QVLNVATIVITGLGTAAVLMYASYRVIDGPLTVGSLWIFVAYMQSLYQMMNEVMFVYG
                     PFQDAVVGVGRVFQALDQVSDVEEARDAEPKPTFDQGIRLRSVWVEYAPGKPVLRGID
                     LEVCKGEKVAIVGETGSGKTTVLNLIPRLFDVSGGAVELDGRDVRRLRIADLRDLVSM
                     VPQEPLLFSASVRENILYGRLDASPEEVEEAARLARADGFIQELPAKYETEVGDRGVR
                     LSTGQQQRISIA"
     gene            complement(1571..>2119)
                     /locus_tag="VNL92_05030"
     CDS             complement(1571..>2119)
                     /locus_tag="VNL92_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94108.1"
                     /translation="RPSEVAKCVALINRTHKGQDLFRPYTADFLRGRLDDPSWGPRPE
                     FWVPVYGWPDYWVLEEHGRVVACAGLWDRGRHIRELWHKPSTGERRALASTALMDWGF
                     AEGHEEAMARLIAFFLGETARLGRDHLLAPIEHHPRLFELLAQYDPAPDTRGMNWRVF
                     DGKGGSLRDPRPSRIYTDLAYW"
BASE COUNT          385 a          725 c          686 g          323 t
ORIGIN      
        1 cgctatcgag atgcgctgct gctgacctgt ggacagacgt acgccgcggt cgccgacctc
       61 cgtctcgtac ttggccggaa gctcctggat gaacccgtcc gctcgcgcca atcgagccgc
      121 ctcctcgact tcctctggcg atgcgtcgag ccggccgtag aggatgttct cgcgaaccga
      181 cgccgaaaag aggagcggct cctgtggcac catgctcacg agatcgcgaa ggtctgcgat
      241 acggaggcga cgcacgtcgc gtccgtcgag ttcgacggcg ccgccgctga cgtcgaagag
      301 cctggggatg aggttgagca ccgtcgtctt ccccgagccg gtctcgccga cgatcgccac
      361 cttctcgccc ttgcacacct cgaggtcgat gccgcgaagc accggcttcc cgggagcgta
      421 ctcgacccat accgagcgaa ggcgaatacc ttggtcgaac gtcggcttcg gctcggcgtc
      481 gcgcgcctcc tcgacatcgg agacttggtc gagcgcctga aacacgcggc caacgcccac
      541 gacggcatcc tggaaaggcc catacacgaa catgacctcg ttcatcatct ggtagaggct
      601 ctgcatgtag gcgacgaaga tccacagcga gccgacggtc agcggtccgt cgatgacgcg
      661 gtacgacgca tacatcagga ccgccgccgt gcccaggccg gtgatcacga tggtcgccac
      721 gttcagcacc tgttcggcga cgcgaaagcg cagctgcgcc tggacgaacg cgaggctcgt
      781 gcgttcgaac cgctggcgtt cgggcccctc ccggccgaac acctgcacgg cacgaatgga
      841 gcccatcgtc tgctcgatga gcgacgtcat gcgggccatg tgctcccggc tctcttgcga
      901 ggtgctctgg attcggcccg tgagcacgtg gatcgtcagg aagagcagcg gtagcgccgc
      961 gagcgcgacg agcgtgagcc gccaatcgag caccgccatg agcgccacca tgagggccag
     1021 ccgaaggatg aggatcgacc aggtccggac cccgaagatc atcacctctt gcacgctccg
     1081 ggcgtcgttg atggcgcgcc agatcgtgtc gcccaaggac tgctgggcgt ggaaccggag
     1141 cgagagccgg cggaggtggg ccatgaggcg cgacctcagc ccgtgaatca tgtagagggc
     1201 cgtccgcgcg atccaataga agctgagggc gccgaggacc acgcccgcga cggtgacgcc
     1261 gaagtatgcg ccggcaaggg cagcggcggc ccgcagcttc tcgccgtcgc tcgccgtagg
     1321 gaactccccg agcggtccga ggtcgagttg actcgagacg agcacgccgt cgatggcgaa
     1381 cttgatcggc cacggctgga gcacaacgaa gagcgagctc gccatcgcga gcgcgacggc
     1441 ggtagcgatg cccccccaga acagcgccat gtagccgagg atgcggcgat acactcctcg
     1501 catcggcgag gccgcgttat cgctcggaag gggtgcgctg gggtcgaacc aatagctcac
     1561 gacttcacca tcaccagtac gcgaggtccg tgtagatgcg cgatggacgc gggtctcgta
     1621 gcgagccgcc tttcccatcg aagactcgcc agttcatccc gcgtgtgtcg ggagccggat
     1681 cgtactgcgc gagcagctcg aaaagccgcg ggtggtgctc gatgggcgcc aggaggtgat
     1741 cgcggcccag gcgcgccgtt tcgcccagga agaacgcaat gagcctcgcc atcgcctcct
     1801 cgtgtccctc ggcgaacccc cagtccatca gagccgtgct cgccagcgca cgccgctccc
     1861 ccgtggatgg cttgtgccat agctcccgaa tgtgccggcc acggtcccac aggccggcgc
     1921 acgcgaccac tcgcccatgc tcctccaaca cccagtagtc gggccacccg tacacgggca
     1981 cccagaactc cggcctcggg ccccacgaag gatcatccaa ccgtccgcgg aggaaatccg
     2041 ccgtgtaggg gcggaacaga tcctgtccct tgtgcgttcg gttgatgagc gccacgcact
     2101 tcgccacctc ggacggccg
//
LOCUS       DATMBB010000229         3976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_159528, whole genome shotgun sequence.
ACCESSION   DATMBB010000229 DATMBB010000000
VERSION     DATMBB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159528"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VNL92_05035"
     CDS             <1..769
                     /locus_tag="VNL92_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94109.1"
                     /translation="LRHGVPAEALEGRGEAAFLSPSLLGGPQDHRRGVDQEVEELEAE
                     HGRRHRRKLAWRTPLRMTAVQPDRLLDDGDVVERCGVTLQTIVCPGHTPANTVYWHEP
                     SGTLFSGDHILSRVTPNPGIHFPDGASRMRSLPAYVRSLQRVRSLPAREVLPGHGEEM
                     SDLPGAIERLLAHHEHRAGRIEAMLLERPHTAYEVMLRIFPRVGPERLRQAMAEIVGH
                     LDLLVEQGVAVELDDGGVVHYRGHSRRPIADLRGTAT"
     gene            865..1347
                     /locus_tag="VNL92_05040"
     CDS             865..1347
                     /locus_tag="VNL92_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWO94110.1"
                     /translation="MANLVRWDPWQELATLRQQMDRLFEDGFGRLMPRGFDESFGIAM
                     DVVETPDRYTVKAAVPGVKPEDVDISVEDGVLTIKGEHREEHRDEKENYLRRELRYGA
                     FERSLRLPPSVDADRAEAHFANGMLTLSLPKRAEAQPKKIRVSAQGSTPAIEGEARQS
                     "
     gene            1551..2321
                     /locus_tag="VNL92_05045"
     CDS             1551..2321
                     /locus_tag="VNL92_05045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003930421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO94111.1"
                     /translation="MGLEGRVAVVSGGGRGIGAGISLALARDGADVAMLYARDAESAQ
                     DTARRIGELGRRAEPIQADIRDEAQAASAIEAAMRALGRIDILVNNAGIASRGLAVAD
                     TDPAEMANVVAVHAFGPFYLTRAALPHLREAAKERGRADVLFISSGATQFFGPNGAPY
                     NMGKAAMEALAFTLAKEERRNNIRVNIVAPGLVETEMGRRLVRATRGVDDMRQLDAHS
                     PFGRVCQPEDIGNMVAFLCSDGASYVTNQRIYVHGGGF"
     gene            complement(2332..2784)
                     /locus_tag="VNL92_05050"
     CDS             complement(2332..2784)
                     /locus_tag="VNL92_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HWO94112.1"
                     /translation="MTDGEAGPRRRYARSPGERRHDGARRQREERRGRFLELVQRAID
                     ELPPAFAEAIENVDIVVASRPTRAELREAGLGPGGVLLGLYQGIPLTRRGSSYNLALP
                     DKITLYQDVIERSCASEEELVHEVRATLLHELAHHFGIDDDRLDELGR"
     gene            complement(2781..3218)
                     /locus_tag="VNL92_05055"
     CDS             complement(2781..3218)
                     /locus_tag="VNL92_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94113.1"
                     /translation="MDNDYGIDIAGIIRTIQSADVMTFRFVILSERLLFDTRSNELDG
                     PMLAIVPRVDSAEERFRSVRRLRPRFANPERISTLWWPRRVESLVSTGVWDAIMRRII
                     ESPFPRCSEVAQRTLGDLVQLQQSEIRHAITGEGYRSLWECRS"
     gene            3350..3829
                     /locus_tag="VNL92_05060"
     CDS             3350..3829
                     /locus_tag="VNL92_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94114.1"
                     /translation="MRPPLFGRLFRRRAIERQIEKCIRDGTGLRQEQWVWPESDAIGE
                     DDLAVMSDAIVQWCRDTMAGLRRPHGIDHVALSVACAHAGSPPMASMTLEVFRPLDFY
                     QEGGPREQLETFIRGLDVQTLNEPRHPIRFAAALFSWGEVTWAALFADDRRRAGVPA"
BASE COUNT          657 a         1285 c         1366 g          668 t
ORIGIN      
        1 gctccgccac ggcgtgcctg ccgaagcgct cgaagggcgt ggggaagccg cgttcctcag
       61 cccatcgctc ctcggcggcc cgcaggacca ccgccgcggc gtggaccagg aggtggagga
      121 actggaggcc gagcacggac gtcgtcatcg gcgcaagctg gcttggcgca cgccgctgcg
      181 aatgacggcc gtgcaaccgg acagactgct cgacgacggc gacgtcgtcg agcgctgcgg
      241 cgtgacgctg caaacgatcg tttgcccggg ccacacgccg gccaataccg tgtactggca
      301 cgagccgagc ggaacgctgt tctcgggcga ccacatccta tcgcgcgtga cgccgaaccc
      361 gggcattcac ttcccggacg gcgcctcgcg catgcggagt ttgcccgcct atgttcgctc
      421 cttgcaacga gtgcgctcgt tgccggcacg ggaggtgctg cccggccacg gggaggagat
      481 gtcggacctg cccggcgcca tcgagcgcct gctggcacac cacgagcatc gcgctggacg
      541 catcgaagcg atgctgctgg agcgaccgca caccgcatac gaggtgatgc tgcggatctt
      601 cccgcgcgtg gggccggagc gtctgcgaca ggcgatggcg gagatcgtcg gtcatctcga
      661 cctgctcgtc gagcagggcg tggccgtcga gctggacgat ggcggggtcg tgcactaccg
      721 agggcactca cgtaggccta tcgccgacct ccgcgggaca gcgacctgac aatcgtgatg
      781 taaaccctct tgacagcatg ctagggtctt gctatcctta cgccgtaatc aaggaagcac
      841 ataggttccc aggaggtatt ggcaatggcg aatctggtac ggtgggatcc ctggcaggag
      901 ttggcgacgt tgcgccagca gatggaccgt ctctttgagg atggctttgg ccgcctgatg
      961 ccgcgcgggt tcgacgagtc cttcggcatc gccatggacg tggtcgagac ccccgatcgc
     1021 tacaccgtca aggcggccgt gccgggcgtc aagcccgaag acgtggatat cagcgtcgag
     1081 gacggtgtct tgaccatcaa aggcgagcac cgtgaggagc atcgcgacga gaaggagaac
     1141 tacctgaggc gcgagctgcg ctatggtgcc ttcgagcggt cgctgcgcct gcctccgtcg
     1201 gtcgacgcgg accgtgccga ggctcacttc gccaacggca tgctgacgtt gtcgctgccg
     1261 aagcgcgcgg aggcgcaacc gaagaagatt cgcgtttcgg cccagggctc gacgcccgca
     1321 atcgagggcg aggcgcggca gagctaaggg atccttccgc ccacaactga atagcgtgac
     1381 ggccccggga cttggtctcg gggctttgct tttgcgcggc cccaagtgcg tgaccaaggc
     1441 gagcttgccc ctggctgagc acgacaccaa ggccgcccta cgccgcccgg gcacttcgga
     1501 tacaataccg cggcagggcc gggacacgtg ttctgcggga ggtgttgctc atggggttgg
     1561 agggaagggt tgccgtggtg agcggaggag gccgaggcat cggcgccggc atctcgctcg
     1621 cgctggcgcg cgatggagcc gatgtcgcga tgctctacgc gcgggatgcg gaatcggcgc
     1681 aggatacggc ccgcagaatc ggagagctag ggcgccgggc ggaaccgatc caggctgaca
     1741 tccgcgacga ggctcaggcg gcctccgcga tcgaggcagc catgcgcgcc ctcggccgca
     1801 tcgacattct cgtgaacaac gccggcattg cctcgcgggg actcgccgtc gccgacaccg
     1861 acccagcgga gatggcgaac gtcgtcgccg ttcacgcctt cgggccgttc tacctcaccc
     1921 gcgcggcgct gccgcacctc cgcgaggcgg cgaaggagcg cgggcgcgcc gatgtgctct
     1981 tcatctcgtc aggtgcgacg cagttcttcg ggccaaacgg cgctccatac aatatgggca
     2041 aggccgcgat ggaggccttg gcgtttacgc tcgcgaaaga ggagcggcga aacaacattc
     2101 gcgtcaatat cgtcgctccc ggcctcgtgg agacggagat gggccggcgg ctcgtccgtg
     2161 cgacgcgtgg cgtagacgac atgcgccagc tcgatgcaca ctcgccgttc gggcgcgttt
     2221 gtcagccgga ggacatcggc aacatggtgg ccttcctttg ctccgatggc gcgagctacg
     2281 tgacgaacca gcgcatctac gtgcacggcg ggggcttctg agccggcgaa cctagcgccc
     2341 gagctcgtcg agacggtcgt cgtcgatgcc gaagtggtgt gccagctcgt gcagtagcgt
     2401 ggcgcgcact tcgtgcacga gctcctcttc ggatgcacag ctccgttcga tgacatcctg
     2461 gtagagcgtg atcttgtccg gcagggccag gttgtaggag ctgccgcgcc gcgtaagcgg
     2521 gattccctga tacaggccca ggagcacgcc gccgggcccg aggcccgctt cccgaagctc
     2581 ggcgcgtgtt gggcgggagg cgacgacgat atcgacgttc tcgatcgctt cggcgaaggc
     2641 tggtggtagc tcgtcgatgg ctcgctggac aagctcgagg aagcgccctc gtcgctcctc
     2701 tcgttgccgg cgagcgccgt cgtggcgcct ttcgcccggc gagcgagcgt aacggcgacg
     2761 aggtcccgcc tcgccgtccg tcacgaccgg cattcccaca aggagcggta gccttcgccg
     2821 gtgatggcgt ggcgtatctc gctctgttgg agttgcacga ggtcgcccag ggttcgctgc
     2881 gccacttcgg agcagcgagg gaacggactc tcgatgatcc tgcgcatgat cgcgtcccaa
     2941 acgccggtcg ataccaggct ctcgacgcgt cgcggccacc acaaggtgct gatgcgctcc
     3001 gggttcgcga accgcggcct cagccgccgc acgctacgaa agcgctcctc ggctgagtcg
     3061 acccggggca cgatcgccag catcggccca tcgagctcgt tcgaccgcgt atcgaacagc
     3121 agacgctcgc tcaggatgac gaagcgaaac gtcatcacgt cggcggactg tatggtgcgg
     3181 ataattccgg cgatgtcgat gccgtagtcg ttgtccattg cgcctcggtt ggctctgggc
     3241 cgcgtcgcga tcctgtaatg acattgtaac gtgaactgca cgtcccacgc ctaccgagcg
     3301 ccggctgttt tgccgatgat gtgagggaga gtacctaggg gagatgcgta tgcggccacc
     3361 actgttcggg cgtctgtttc gccgccgagc gatcgagcgc cagatcgaga aatgcatccg
     3421 cgatggcacc ggtctgcgcc aggagcagtg ggtgtggccg gagagcgacg ccatcggcga
     3481 ggatgacctc gcggtcatga gcgacgcgat cgtccagtgg tgccgggata ccatggcagg
     3541 tttgcgccgg ccgcacggca tcgatcacgt ggcgctttcg gtggcctgcg cccatgccgg
     3601 cagcccccca atggcgagca tgacgctgga ggtgttccgg ccgctcgact tctaccagga
     3661 gggcggtcca cgggagcaac tggagacgtt cattcgcggc ctcgatgttc aaaccctcaa
     3721 cgagcctcgc cacccgattc gcttcgcggc cgcgctgttc agctggggcg aagtgacctg
     3781 ggcggcgctc ttcgccgacg atcggagacg cgcaggcgtt ccagcctagc cctcccgcag
     3841 catccgctct tccagcaacc agcggtcgaa ctcgccctcc gggtcctcga cacccaggtc
     3901 gttcgcggcg cggcgccggc tatggatgcc ggcctcgacg agcgagcgct ggtcggcaac
     3961 catgcggctc cgatct
//
LOCUS       DATMBB010000230         2820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_42012, whole genome shotgun sequence.
ACCESSION   DATMBB010000230 DATMBB010000000
VERSION     DATMBB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42012"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="nuoH"
                     /locus_tag="VNL92_05065"
     CDS             complement(<1..1019)
                     /gene="nuoH"
                     /locus_tag="VNL92_05065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWO94115.1"
                     /translation="MTLLGQWYDIRDLSELVAGLSGWLRDTFDAGDGVIYVVSALLGA
                     AGILAFIGGTAIVNIWIERRLVGRMQNRLGPNRVGPFGLLQPVADAIKLIQKEALMPR
                     VADPVVFTLAPILVFVPAILAWAVLPWGEHMTLASLDAGVLYFVALGTVAVLAVFMAG
                     WSSNNKFSLLGAMRVVPMLLSYEIPIVLAFLGPVLLAGTMNLSGIARWQADYHVWIIF
                     LQPLTFLIYFIAASAELNRTPADIAEAESEILAGYHTEYSGMKFGLFYAVELVNALLV
                     SGLAASMWYGGWYLFEIDRVVPGWLIFIGKMYALYFLLIWTRGTLPRLRIDQLVALSW
                     KFMTPL"
     gene            complement(1016..2119)
                     /locus_tag="VNL92_05070"
     CDS             complement(1016..2119)
                     /locus_tag="VNL92_05070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWO94116.1"
                     /translation="MPIKIEPYVVNIGPQHPSTHGVFRMRVTLDGETITDAEMIVGYL
                     HRSMEKLAEERTYTQNIPFTDRMDYLAAMTGNLGYCLAVEKLAGIEVPERATVLRVIF
                     AELQRIASHCMANGVFINDCGAWQTPLMYMFREREKILDLFEMTCGARLTTNYMRIGG
                     VAFDIPDEFLPRLRALIDELPERIAEYEELLLDNEILLLRGRDVGVITKELAVNASLS
                     GPMLRSTGIAWDVRKGDPYCGYETYDFEIPVGHNGDAYDRFVVRMEEMKQSVRIIEQA
                     IERLPDGPYKADVPLAIRPPACEVYSRIESPKGELGFYLVSDGGSAPWRFHVRSPSLI
                     NLSALREMVVGLGVADAIVAFGSVDINVGELDR"
     gene            complement(2121..2693)
                     /locus_tag="VNL92_05075"
     CDS             complement(2121..2693)
                     /locus_tag="VNL92_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWO94117.1"
                     /translation="MTRRLSGFEVAERIRTRFPDAVVEETADWVVLRPDNLVEVMRFI
                     RDDEELDAKFLNMLCGVDRIEDFEVVYHVSSLTKNHTFALKVRADHDRPSVPSVVPVW
                     LGAHLQEREAYDLMGIRFEGHPSLKRLFLWEGFPGHPLRKDFVRLPGGQKPGLARFPK
                     EMPGRTGDEFRPELGGPAHDLPGDEPYMKD"
     gene            complement(2693..>2820)
                     /gene="ndhC"
                     /locus_tag="VNL92_05080"
     CDS             complement(2693..>2820)
                     /gene="ndhC"
                     /locus_tag="VNL92_05080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWO94118.1"
                     /translation="PWAVSLRQVKLFGFVEAMIFIAILLVGYLYAWRKRALEWRY"
BASE COUNT          513 a          903 c          957 g          447 t
ORIGIN      
        1 aggggcgtca tgaacttcca cgacagcgcc acgagctggt cgatgcgcag ccgcggcagc
       61 gtaccgcgcg tccagatgag caggaagtac agggcgtaca tcttgccgat aaagatgagc
      121 cagcccggca cgacgcggtc gatctcgaac aggtaccacc cgccgtacca catcgacgcc
      181 gcaaggcccg aaaccaggag cgcgttcacc agctcgacgg cgtagaagag cccgaacttc
      241 atgcccgagt actcggtgtg gtagccggcg aggatctcgg attcggcctc ggcgatgtcc
      301 gccggcgtgc ggttcagctc ggccgacgcg gcgatgaagt aaatcaggaa ggtcagaggt
      361 tgcaggaaga tgatccagac gtggtagtcc gcctgccagc gagcgatgcc cgacaggttc
      421 atggtccccg ccagcaagac cggtcccaaa aacgcgagga cgatcggaat ctcgtaactc
      481 aggagcatgg gaacgacgcg catcgctccg agcagggaga acttgttgtt cgacgaccag
      541 cccgccatga atacggcgag cacggcgacc gttcccagcg cgacgaagta caggacgccg
      601 gcgtcgaggc tcgcgagggt catgtgctcg ccccagggaa gcaccgccca cgcgaggatg
      661 gccggcacga acacgagaat gggcgcgagg gtgaacacga cggggtcggc cacccgcggc
      721 atcagcgctt ccttctgaat gagcttgatg gcgtcggcga ccggctggag cagcccgaag
      781 ggaccgaccc ggttgggtcc caggcggttc tgcatgcggc cgaccagcct gcgctcgatc
      841 cagatgttga cgatggcggt gcccccgatg aacgccagga tgccggcggc gcccagcagc
      901 gcggagacga cgtagatcac gccgtcgccg gcgtcgaacg tgtcgcgcaa ccacccagag
      961 agcccggcga cgagctcgga caggtcgcga atgtcgtacc actgtcccag aagcgtcacc
     1021 ggtcgagctc tcccacgttg atgtcgacgc tcccgaacgc gacgatggcg tcggcgacgc
     1081 cgaggccgac caccatctcg cggagcgcgc tcaggttgat gagcgatggc gagcgcacgt
     1141 ggaaccgcca gggcgcggag cccccgtcgc tcaccaggta gaagccgagc tcgcccttcg
     1201 gcgactcgat gcggctgtac acctcgcagg caggcgggcg gatggccagc ggcacgtcgg
     1261 ccttgtacgg cccgtcaggg aggcgctcga tcgcctgctc gatgatgcgc acgctctgct
     1321 tcatctcctc catgcgcacg acgaaccggt cgtaggcgtc gccgttgtgg cccaccggaa
     1381 tctcgaaatc gtacgtctcg tacccgcagt acgggtcgcc cttgcgcacg tcccaggcga
     1441 tgcccgtcga gcgcaacatg ggcccgctga gggaggcgtt gacggcgagc tcctttgtga
     1501 tcacgcccac gtcgcggcca cggaggagga ggatctcgtt gtcgagcagg agctcctcgt
     1561 actccgcgat acgctcgggg agttcgtcga tcagggcgcg caggcgcggg aggaactcgt
     1621 ccgggatgtc gaacgccacg ccgccgatgc gcatgtagtt cgtggtgagg cgggcgccgc
     1681 aggtcatctc gaacaggtcg aggatctttt cgcgctcgcg gaacatgtac atgagcgggg
     1741 tctgccaggc gccgcagtcg ttgatgaaga caccgttggc catgcagtgg ctggcgatgc
     1801 gctgcagctc ggcgaagatc acccggagca ccgtcgcccg ctcgggaacc tcgatgccgg
     1861 cgagcttttc gacggcgaga cagtacccca gattgcctgt catcgccgcg agatagtcca
     1921 tccggtcggt gaacgggatg ttctgcgtgt acgtccgctc ctccgccagc ttctccatgc
     1981 tgcggtgaag gtacccgacg atcatctcgg cgtcggtgat cgtctcgccg tccagggtga
     2041 cccgcatccg gaacacgccg tgggtgctgg ggtgctgcgg gccgatgttg acgacgtagg
     2101 gctcgatctt gatcggcatg tcagtctttc atgtacggct cgtcgcccgg gaggtcgtgc
     2161 gccgggccgc ccagttcggg gcggaactcg tctcccgtgc ggccgggcat ttccttgggg
     2221 aaccgggcga gtcccggctt ctgcccgccc ggcagccgca cgaagtcctt gcgcagcggg
     2281 tgccccggaa agccctccca caggaagagg cgcttgagcg acggatgtcc ctcgaaccga
     2341 attcccatca ggtcgtacgc ctcgcgctcc tggagatgcg cgccgagcca gaccggaacg
     2401 acgctgggga ccgacggccg gtcgtgatcg gcgcgcacct tgagggcgaa cgtatggttt
     2461 ttggtgagcg acgagacgtg atagacgacc tcgaagtcct cgatccggtc gaccccgcag
     2521 agcatgttca ggaacttggc gtcgagctct tcgtcgtcgc ggatgaagcg catgacctcg
     2581 accaggttgt cgggccggag gacgacccag tcggctgtct cctctacgac agcgtccggg
     2641 aagcgggtgc ggatgcgttc ggcgacctcg aagccggaaa gcctgcgggt catcagtatc
     2701 gccactccag ggcgcgtttg cgccaggcgt acaggtagcc gacgagcaga atcgcgatga
     2761 agatcatggc ctcgacgaag ccgaagagct tcacctggcg caggctcacc gcccacggat
//
LOCUS       DATMBB010000231         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_565764, whole genome shotgun sequence.
ACCESSION   DATMBB010000231 DATMBB010000000
VERSION     DATMBB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565764"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VNL92_05085"
     CDS             complement(<1..1110)
                     /locus_tag="VNL92_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWO94119.1"
                     /translation="MTQQSYDERIERLRALGAAMFGEHRLIIASNRGPIEYDLSPSGD
                     ILEGRGSGGVVTALSAIARYMPLTWISATMTEGDYAAYERAGGRAERAPFEDQEMYIR
                     LVAPPRATYQRHYNVFCNPLLWFVQHYMWNTPRMPNISRAVYDAWDSGYVVVNRMFAD
                     AIVDEADRLQDGRCFVMLHDYHLYLAPGMVRERLPEARIQHFTHIPWPDPRYWQLIPS
                     FMRSEIHAKLAAADIAGFQTRRDVRNFLLCCEAFLGAEVDYRTNEVIHAGHRTYVAAY
                     PISVDARGLEELAASDEVERYSARLAPMFGPQTIVRVDRTEPSKNILRGFRAFELLLS
                     RYPDLRGRVQMVAFLVPSRSELAIYQTYTDEIFETA"
     gene            1286..2131
                     /locus_tag="VNL92_05090"
     CDS             1286..2131
                     /locus_tag="VNL92_05090"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HWO94120.1"
                     /translation="MLLTVRPWVDVGSVGTQTLAFLAAQWGAKKLGQLRRPGLYYDFT
                     RYRPLIGNVAGERVVTVPNSTIHCATSDSDWLLVDVMEPHAHGEDFCDAIIDVMRRFE
                     VSEYLMIGAMYGPVPHTRPAVLSGGATDEGLGARMQRLGIRASSYEGPTTILATIPQE
                     VRSLGAATGTLLVQLPAYARLDPDIHGRYALLRTLSDLFDLGFELDRMRREGEQQYDS
                     LEVALAQNPELAGWVRELESVYDRTVGRPAAPEEAPGGEPSQLSPELERFLADMERRF
                     QAPED"
     gene            complement(2128..>2412)
                     /locus_tag="VNL92_05095"
     CDS             complement(2128..>2412)
                     /locus_tag="VNL92_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94121.1"
                     /translation="GWIVLAAAVIAIIGAALRMRPVIGPGVWQVWAFACAAFGGVAAF
                     VNAVHVTAAGDFELADISFGVVVSLVAAVSMLAFTVADGEPEPGTDGARA"
BASE COUNT          415 a          761 c          814 g          422 t
ORIGIN      
        1 cgccgtttcg aagatctcgt ccgtgtacgt ctggtagatc gccagctcgg agcgcgaggg
       61 gacgaggaag gcgaccatct gcacccttcc ccggaggtcg ggatagcggc tcaggaggag
      121 ctcgaaggcg cggaagcccc gcaggatgtt cttgcttggc tccgtgcggt ctacgcgcac
      181 gatggtctga ggtccgaaca tcggcgccaa cctggcgctg taacgttcca cttcgtcgga
      241 agcggcgagc tcctcgagcc ctcgtgcgtc gaccgagatg gggtaggcgg cgacgtatgt
      301 ccgatggcct gcgtggatga cttcgttggt ccgatagtcg acttcggccc cgaggaaggc
      361 ttcgcagcag aggaggaagt tccggacgtc gcgccgagtc tggaagcccg cgatgtcggc
      421 ggcggctaat ttcgcgtgga tctcgcttcg catgaacgag gggatgagct gccaataccg
      481 ggggtcaggc catgggatgt gggtgaagtg ctggatgcgc gcttccggca accgctcgcg
      541 taccatcccg ggcgccaggt acaggtgata gtcgtgcagc atcacaaagc atcggccgtc
      601 ctggagccga tcggcttcgt cgacgatcgc atcggcgaac attcgattga ccacgacata
      661 gccggagtcc catgcgtcat acactgcacg gctgatgttc ggcatgcgcg gcgtattcca
      721 catgtaatgc tggacgaacc agaggagcgg attgcagaag acattgtagt ggcgctggta
      781 ggtcgcgcgc ggcggagcca cgagccgtat atacatctcc tggtcttcga agggggcccg
      841 ctccgcgcgt cctcccgccc tttcgtaggc ggcatagtcg ccctccgtca tcgtcgcgga
      901 tatccacgtg aggggcatgt atctcgcgat cgcgctgagg gccgtgacga ccccgccgct
      961 tccgcgcccc tcgaggatat ctccggaggg cgagaggtcg tattcgattg gcccacggtt
     1021 gcttgcgatg atgaggcgat gctcgccaaa catggccgcg ccgagcgctc gcaggcgttc
     1081 gatgcgttcg tcgtaggatt gctgcgtcat ggcggcaccc cagcgaccct cacgctagca
     1141 tcgggccttt cgaggtgtca atttcgccgg gcgctgccgc cttgcgcggt cgcggtaaga
     1201 tcttcgcaaa gagtcacggg aggacatcat ttccgggcac acgttcgacc ttatcgagcc
     1261 tccgccgagc ctacgcagtc cccgcatgct actcaccgtg cgaccgtggg tggatgtggg
     1321 ttccgtgggg acgcaaacgc tcgccttcct ggcggcacaa tggggcgcca agaagctggg
     1381 acagctgcga cggccgggct tgtactacga tttcacacgc taccgtccgc tcattggcaa
     1441 cgtggcaggg gaacgcgtgg tcaccgtccc gaactccacg attcattgcg cgactagcga
     1501 ttcggactgg cttctggtgg acgtcatgga gccgcacgcc cacggcgaag acttctgcga
     1561 tgccatcatc gacgtcatgc gtcgattcga ggtgtcggag tacctgatga tcggcgccat
     1621 gtatggaccg gtgccgcaca cccggccggc ggtcctgagc ggcggcgcaa ccgacgaggg
     1681 actaggggcg cgcatgcaga ggcttggcat ccgcgccagc tcatacgagg ggcccacgac
     1741 gattctcgcg accattcctc aggaggtgcg ttcgctggga gcggcaaccg gcaccctgct
     1801 cgtgcagctt cccgcctacg ctcgtctgga ccccgacatc cacgggaggt acgcgttgct
     1861 ccgcacgctc agcgacctgt tcgacctcgg cttcgaactc gaccgcatgc ggcgtgaggg
     1921 cgagcaacag tacgattcgc tcgaggtggc gttggcccag aatccggagc tcgccggatg
     1981 ggtgcgcgag ctggagtcgg tctacgaccg cactgtgggc aggccggccg cgccggaaga
     2041 ggcgcccgga ggtgaaccga gccagctttc gccggagctc gagcgctttc tggcggatat
     2101 ggagcgtcgc ttccaggcgc ccgaggatta ggccctcgcg ccgtccgttc ctggctccgg
     2161 ctcgccatcg gcgaccgtga aggcgagcat ggacacggcg gcaacgagcg aaacgactac
     2221 cccgaacgag atgtcggcca gctcgaaatc gccggcggcg gtgacgtgca ccgcgttgac
     2281 gaacgccgcg acgccaccga acgcggcgca cgcgaacgcc cacacctgcc agacgccggg
     2341 cccgatcacg ggtcgcatgc ggagcgctgc tccgatgatg gcgatgacgg cggcggcgag
     2401 cacgatccag cc
//
LOCUS       DATMBB010000232         5646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_121592, whole genome shotgun sequence.
ACCESSION   DATMBB010000232 DATMBB010000000
VERSION     DATMBB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121592"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VNL92_05100"
     CDS             complement(<1..1005)
                     /locus_tag="VNL92_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94122.1"
                     /translation="MGQDTVSTEAGPGGPLRNVRVLDLTTERGAYATKLLADLGADVI
                     RVEPPAGDSMRDLPPFLDDERHRDRSLTHWFYNSGKRSIIADLHVESDRDVVRRIAGR
                     VDILVESHDVGSLASLGLGHDDMLAANDRLIYVSITPFGQQGPWRDYRADDFVLCALG
                     GMMGVCGDPDRPPLVAFGWQAYHTAGNFAALSAMAALRSRRSMGRGQYVDVSIEACVA
                     GFLEHVNVFYLHQGSFGALSGDPVARRQGPLHWSLGFRTYRTADGYALLSHFHQWDDL
                     IAWLAADDMAEDLTDPRYDDPRVRRTDVHHVCEVIARWAATKRTEQLVNEGQLRRFPW
                     A"
     gene            complement(987..1211)
                     /locus_tag="VNL92_05105"
     CDS             complement(987..1211)
                     /locus_tag="VNL92_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94123.1"
                     /translation="MNDEPTPKRYAADCRFLQRTFDSSRIDSFVTRCRHIVRDGEDCV
                     GPFLDETETRCQLWEPLPIGEPVEPWGKIP"
     gene            complement(1324..2112)
                     /locus_tag="VNL92_05110"
     CDS             complement(1324..2112)
                     /locus_tag="VNL92_05110"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94124.1"
                     /translation="MPPNSVHVAGLQHTFAAAGGAGIAALGGVTLDVAPGSFVAIVGP
                     SGCGKSTLLRILAGLLVPSAGQASIGERSVLGRPGLAAYMPQRDALLPWRRALENATL
                     GPEVARRNVREKRERAGALFGRFGLAGFERAWPAQLSGGMRQRLALLRTFLIDASVLL
                     LDEPLGALDAITRRSMHQWLESVWLEDGRTTILVTHDVDEALLLADRIFVMTPRPGRV
                     ALAIDVPQPRPRRPDVVTTPGFVTLKADVLAALDATMALDTSAV"
     gene            complement(2099..3169)
                     /locus_tag="VNL92_05115"
     CDS             complement(2099..3169)
                     /locus_tag="VNL92_05115"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94125.1"
                     /translation="MKHRLARLAPLLALVALGFALSAGCGDDDDDGAAAETDGGGEDL
                     RSVTLMLDWTPNTNHSGIYVAQDKGWYEEQGLRVDIIEPAAGGTEQAVAGGQAQFGIS
                     VQEQVIPARQQGIPVVSIAAIIQHNTSSLMSLAEDGIERPADLPGHTYGGFGGALETA
                     LIRTLVTCDGGDPDGVEFVEVGNADYLVGMEEDQFDVVWIFDAWDGLRATDVEQRDVN
                     FIHFADWFDCIPDWYTPVFITSQDMIESDPDTVRAFMEATSRGYEFAMENPEETADIL
                     LRAAPELDERLVRASAEWLATRYVDEGRQWGLQDREVWDRFHEFVLNAGLIEEPIDVE
                     AAYTNDFLPDNEGSMDAGDATQ"
     gene            complement(3166..4029)
                     /locus_tag="VNL92_05120"
     CDS             complement(3166..4029)
                     /locus_tag="VNL92_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94126.1"
                     /translation="MPSSNAIDQTPSLHVPDVPPKRVPSLVQAAGRATAQYLPAIALL
                     IALIFLWEFLVRWLDTRPYLLPAPSRIWDAFVDVRDVLPRHVRATMYEAIVGLIIAAA
                     SGAVLAGLIATIPLVRRVLYPIVIASQTIPIIVLAPLLVTWFGFGLAPKVTVVALAGF
                     FPVVVSTADGLMQADRELIGLVRSMGGGRYQVMRFVLVPSALPSFFAGMKIAAAYAML
                     AAIIGEWVGAREGLGVFITRSQASFRTDQIFVAIAIIALASMALFAVVTILARIATPW
                     MYIGGKEDGER"
     gene            complement(4033..4329)
                     /locus_tag="VNL92_05125"
     CDS             complement(4033..4329)
                     /locus_tag="VNL92_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HWO94127.1"
                     /translation="MIAEVECIPTPAGTESEPYRFVDAAIAVIQQSGLHYEVSALGTT
                     LEGEPDQVWDTVRRTHEACLAAGARGLLTIVKFEQVAEGREQPTMDSLTKKFRQ"
     regulatory      complement(4413..4518)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4572..>5646)
                     /locus_tag="VNL92_05130"
     CDS             complement(4572..>5646)
                     /locus_tag="VNL92_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94128.1"
                     /translation="FLRKETQQPTGPQLQLTWMHAKTERGARARASNPSRGLTINDIN
                     VGGYGDIPDSWHIETQLPRGAMPPADSLPSLGYSIYDKVDQWADSCGDLYEEAIQRRW
                     APALDIPWDTVQPLPEEIERAMCQICTELSEAALHNVRVLGRWLQEFAYGYHEVKLYL
                     ATEVYSHARHFEGLRKRALVNGGGLGVEAPGMYLRNINDARKWTDLECAMFIVQATFH
                     QGYCLTLARYAHNEAERRLFTLMAQDLARSITYGMEQIRLQLRREPRRRNEINSYLNR
                     SEAILMAEIERDVPLTEAFEVVLGGGIENAATIGKQRLEEMRRWQVERYLKRVAAMGL
                     PERAENPSPQLAKYLPQREAQPV"
BASE COUNT          937 a         1846 c         1899 g          964 t
ORIGIN      
        1 cgcccatggg aagcggcgga gctggccttc gttcacgagc tgttcggtgc gcttggtcgc
       61 tgcccaccgc gcgatgacct cacagacgtg gtgcacgtcc gtacggcgca cacgcgggtc
      121 gtcgtaccgc ggatccgtga ggtcctctgc catgtcatcg gcggcgagcc aggcgatgag
      181 gtcgtcccac tgatgaaagt ggctcaggag cgcgtagccg tcggccgtcc gataagtgcg
      241 aaagcccagg ctccaatgca gggggccctg tcgccgcgcc accggatcgc cggagagggc
      301 gccgaaggat ccctggtgca gatagaacac gttgacatgc tcgaggaagc cggcgacgca
      361 cgcctcgatg ctcacatcca cgtattggcc gcgccccatc gatcgcctgc tgcgcagcgc
      421 cgccatcgcg ctcagtgccg cgaagttgcc agccgtatgg tacgcttgcc agccaaaggc
      481 gacgagcggc ggacgatcgg ggtcgccgca cacccccatc atgcctccga gggcacagag
      541 cacgaagtca tcggcgcggt aatcacgcca cgggccttgc tggccgaacg gcgtgatcga
      601 tacgtagatg aggcggtcgt tcgccgccag catatcgtcg tgcccgaggc cgaggctcgc
      661 cagagatccg acgtcgtggc tctccacgag gatgtcgacg cgcccagcga tgcgccgcac
      721 gacgtcccgg tccgactcga cgtgcagatc ggcgatgatg ctgcgcttgc cgctgttgta
      781 gaaccagtgc gtgagcgagc ggtcacggtg ccgttcgtcg tcaaggaagg gcggcaggtc
      841 tcgcatggaa tcgcccgcgg gcggctcgac ccgaatgacg tcggcaccga ggtcggcgag
      901 caatttcgtc gcgtaggcgc cgcgctcggt cgtcaggtcg aggacgcgta cgttccgcag
      961 tgggccgccg ggaccagcct ccgtgctcac ggtatcttgc cccaaggctc cacgggctca
     1021 ccgatgggca gcggctccca aagctggcag cgcgtctcgg tctcgtcgag gaacggccct
     1081 acgcaatcct cgccatcgcg aacaatgtga cggcatcgcg tcacgaacga atcgatccgc
     1141 gacgaatcga acgtgcgctg gaggaagcga caatcggcgg cgtaccgctt cggggtcggt
     1201 tcgtcgttca tgccggcgcc gccactgcga cctcggattc cgtgatgaag tgaggcatgg
     1261 gtgagtaaga tcctgccgca gcatcgtacg gcatgccgcc gggcgtgaca aaccggacgc
     1321 gagttagacg gccgatgtgt cgagcgccat cgtcgcatcg agggcggcga gcacatcggc
     1381 cttgagggtg acgaagcccg gtgtcgtgac cacgtccggc cggcgtggcc gcggctgtgg
     1441 cacgtcgatg gcgagggcca cgcggcccgg gcgcggggtc atgacgaaga tgcggtcggc
     1501 gagaaggagg gcctcgtcga cgtcatgggt aacgagaatc gtggtccgcc cgtcctcgag
     1561 ccacacgctc tcgagccact ggtgcatgct gcgtcgcgtg atggcatcga gtgcgccgag
     1621 cggctcgtcg agcaggagca cgctcgcgtc gatgaggaag gttctcagca gggcgaggcg
     1681 ctgacgcatg cctccgctca gctgggcggg ccacgcacgt tcgaatcccg ccaggccgaa
     1741 cctcccgaac aaggctcccg ctcgctctcg cttctcgcgc acattgcgcc gcgccacctc
     1801 ggggccgagc gtcgcgttct cgagggcgcg ccgccaggga agcagcgcat cacgctgagg
     1861 catgtaggcc gcgagcccgg ggcgtccgag gaccgagcgc tcgccaatcg atgcctggcc
     1921 agctgatgga acgagcagac ccgccaggat gcggagtagc gtgctcttcc cgcagccgct
     1981 cggcccgacg atggccacga acgagcccgg cgccacatcg agggtcacgc cgccgagcgc
     2041 agcgattccg gcgcctccag cggcggcgaa cgtgtgctgc aggcccgcga cgtgtacgct
     2101 attgggtggc atcgccggcg tccatcgacc cctcattgtc cggcaggaag tcattcgtgt
     2161 aggcagcttc gacgtcgatt ggctcctcga tcagccccgc gttcaggacg aactcgtgga
     2221 atctgtccca gacctcgcga tcctgaaggc cccactgtcg cccctcatcg acgtatcgag
     2281 ttgcaagcca ttcggcgctt gcgcggacga gccgctcgtc gagctcgggc gccgcgcgca
     2341 gaaggatgtc cgcggtctct tcggggttct ccatggcgaa ctcgtagccg cgagacgtgg
     2401 cttccatgaa ggcgcgcacg gtatcggggt cgctctcgat catgtcctgg ctcgtgatga
     2461 agacgggcgt gtaccagtct ggtatgcagt cgaaccagtc ggcgaagtgg atgaagttca
     2521 cgtctcgctg ctcgacgtcc gtcgcacgca agccgtccca ggcgtcgaag atccagacga
     2581 cgtcgaattg gtcctcctcc atgcctacga gatagtctgc attgcccact tcgacgaact
     2641 cgacgccgtc ggggtcaccc ccatcgcagg taacaagtgt gcggatgagc gctgtctcta
     2701 acgctccacc gaagccgccg taggtgtgcc cgggcaagtc ggccggccgc tcgatgccat
     2761 cctcggccag ggacatgagg ctcgatgtgt tgtgctggat gatggccgcg atggacacga
     2821 cggggatgcc ctgctgacgg gccgggatca cttgctcctg gaccgagatg ccgaattgcg
     2881 cctgtccacc ggccacggcc tgctcggttc cgccggcggc cggctcgatg atgtcgaccc
     2941 gcaggccctg ctcctcgtac cagcccttgt cctgcgccac gtagatgccc gagtgattcg
     3001 tgttcggcgt ccaatccagc atgagcgtca cgctgcgtag atcctcgcca cctccgtccg
     3061 tttccgcagc ggccccgtcg tcgtcatcgt cgccgcagcc tgccgacagc gcgaacccga
     3121 gcgcgacgag cgcaagcagc ggtgccaagc gcgccaagcg gtgtttcatc gttcaccatc
     3181 ctccttcccg ccgatgtaca tccatggagt tgcgattcgc gccaggatcg tcaccacggc
     3241 gaacagcgcc atgctcgcga gggcgatgat cgcgatggcg acgaagatct ggtccgttcg
     3301 gaaggaggcc tgcgagcgcg tgatgaacac accgagtccc tcccgggctc cgacccattc
     3361 gccgatgatg gcagcaagca tcgcgtaggc tgcggcgatc ttcatgccgg cgaagaagga
     3421 gggcaacgcg gaggggacga gcacgaaccg catgacctga tacctccccc cacccatgga
     3481 gcgaacgagg ccgatgagct cgcgatcggc ctgcatgagg ccatccgcgg tgctcacgac
     3541 gacggggaag aaccccgcga gcgccacgac cgtcaccttc ggcgctaggc cgaatccgaa
     3601 ccatgtgacc aagagcggtg cgagcacgat gatgggaatc gtttgcgacg cgatgacgat
     3661 ggggtagagg acgcgccgca ccagcggaat cgtcgcgatg agtccggcga gcacggcgcc
     3721 gctcgccgcc gcgatgatga gacctacgat cgcttcgtac atcgtcgcac ggacgtggcg
     3781 tggcaggacg tcgcgcacat cgacgaaggc atcccaaatg cggcttggcg cgggcaggag
     3841 gtacggtcgc gtgtcgagcc agcgcacgag gaactcccac aggaagatga gcgcgatgag
     3901 cagcgcgatg gccggcagat actgagccgt ggctcggccg gcggcctgga ccaacgacgg
     3961 cacgcgcttc ggcggaacgt ccggcacgtg cagcgatggc gtctgatcga tcgcgttgga
     4021 tgaaggcata ggctactgcc ggaacttctt cgtcaggctg tccatggtgg gctgctcgcg
     4081 gccttccgcg acttgctcga acttgacgat ggtgagcagg ccgcgagcgc ctgccgcgag
     4141 gcacgcctcg tgagttcgtc gcaccgtgtc ccaaacctgg tccggctcgc cctcgagcgt
     4201 cgtcccaagg gcgctcacct cgtagtgcag gccggactgc tggatgacgg cgatggccgc
     4261 atcgacgaag cggtagggct cgctctccgt tccggcgggg gttgggatgc actcgacttc
     4321 tgcgatcatg gttcctccag ctcctccgtc agcacgacgg gcttacgacg acaaaacgac
     4381 cgtcccgcag ggaagcggga cggtcgttca tgtgtggcgc cggtccgctt tccctacgct
     4441 cgcattaccg agatcaggtt cgaagggtcg gcgctcgttg cgcctctcag ccggaaaccg
     4501 gctcccctag cggtcgatat tcgtttgtcg gccctatgct accacatcgg cagcatccgt
     4561 gcggtcggtt ctcagacggg ctgtgcctcc cgttgcggca ggtacttcgc gagctgcggg
     4621 ctcgggttct cggcacgctc cggcaggccc atggcggcga cacgcttgag gtaccgttcg
     4681 acctgccatc gccgcatctc ctcgagccgc tgcttgccga tcgtggctgc gttctcgatc
     4741 ccgccgccga gcacgacctc gaaggcttcg gtgaggggca cgtcccgctc gatctcggcc
     4801 atcaggatag cctccgaacg gttgaggtac gaattgatct cgttgcggcg gcggggctcg
     4861 cggcgcagct ggaggcggat ctgctccatg ccgtaggtga tgctgcgtgc gaggtcctga
     4921 gccatgagcg tgaagagcct gcgctccgcc tcgttatgcg cgtaccgggc aagcgtcaga
     4981 cagtatccct ggtggaacgt ggcctggacg atgaacatcg cacattcgag atcggtccac
     5041 ttgcgggcgt cgttgatgtt gcgcaggtac atgccgggcg cctcgacgcc gagtccgccg
     5101 ccgttgacca gcgcccgctt ccgaagaccc tcgaagtgac gagcgtggga gtagacctcc
     5161 gtggcgaggt agagcttcac ctcgtgatag ccgtacgcga actcctggag ccagcggccc
     5221 agcacacgca cgttgtggag cgccgcctcg gagagctcgg tgcagatctg gcacatggcc
     5281 cgctcgattt cctcgggcag cggctggacc gtgtcccagg gaatgtcgag cgccggcgcc
     5341 catcggcgct gaatcgcctc ctcgtagagg tcgccgcagc tgtccgccca ctggtcgacc
     5401 ttgtcgtaaa tggagtagcc caggctcggc agggagtcgg ccggcggcat cgctccgcgg
     5461 gggagctgcg tttcgatgtg ccagctgtcg gggatgtcgc cgtagccgcc cacgttgatg
     5521 tcgttgatgg tgagcccgcg ggaaggattc gaggctctcg cccgtgcgcc tcgctccgtc
     5581 ttggcgtgca tccaggtgag ttgcagttgc gggcccgttg gctgctgcgt ttcctttctc
     5641 aggaag
//
LOCUS       DATMBB010000233         3248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_436306, whole genome shotgun sequence.
ACCESSION   DATMBB010000233 DATMBB010000000
VERSION     DATMBB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436306"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VNL92_05135"
     CDS             <1..471
                     /locus_tag="VNL92_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HWO94129.1"
                     /translation="IEGFGSAGGLVPTSEPGGNVTLGARAMQGALRGAGRLQGEVDWI
                     LAHGSGLPRGDAVEVEAIRRVFGPAAANPRVSSVKGAIGHTLGASGALSAAIAVMALT
                     ESSIPPTSGLTDPDPQFDLDFVVSRPGLGALGVVVVNSFSTLGQHASLVLTRAS"
     gene            529..1170
                     /locus_tag="VNL92_05140"
     CDS             529..1170
                     /locus_tag="VNL92_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94130.1"
                     /translation="MDLTWLGHGCIRMRGRETTILCDPCSKDTGYNIGRQAADIVTLS
                     HDHPAHSNVAVVSGHPKVIAGPGEYEVGGALIIGVRTYHDAQSGALRGKNTAYLIEID
                     GTRVCHLGDIGHLPSEQQIEQLVGADILVVPVGGGTTIDAQVAARTVSLLEPRIVVPV
                     HYATPASRVEGLDGVERFLKEMGVTDVTPESKISTGRGGVSGETRTVVLEYRA"
     gene            complement(1230..2678)
                     /locus_tag="VNL92_05145"
     CDS             complement(1230..2678)
                     /locus_tag="VNL92_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HWO94131.1"
                     /translation="MRRPYLHLVALALGIAGVVLGRVANGAFDGDGLPHLAEAPAISL
                     PGIAPGAQIREVAPAPSVTLEEQDAVLVAPALPETVEGETELQEIATSTPEAATDVRA
                     RVDVLPDVEAAEAPQEETVVPEEQPVEEPTPEPEQPIEEEPPAPAEEDPIRVDATTPE
                     QEAQNTGAAGQQGAYFNYIVLPGDTVNSIAAQFGLLSNTILAANLDLVGAETLQPGQN
                     IRIPLADGVLHPVQPGDTIAGLAEQYGTTVEAIVGFAPNGLTSPDDIRIGQELLVVGG
                     TRSFAPPEPAAEETPAETAPEPVDEGNPFAADPAAPSEGAPAAEGEAEVPIVGPEAEP
                     PNPFAAEPPPPEPSPTEAPAVEAGGQPSFVWPFSGNISSYFGPGHPLGIDIDGFQNPT
                     GPVAAAAAGTVTFAGGDPGYSYGYYVIISHGGGWETLYAHLSSISVSVGQVVSAGEAI
                     GIVGTTGYSTGVHLHFEVRQGGAHLNPLVFLP"
BASE COUNT          515 a         1001 c         1159 g          573 t
ORIGIN      
        1 atcgaaggct tcggctccgc tgggggcctc gtgcccacct cggagcctgg aggcaacgtg
       61 acactcggtg cccgggcgat gcagggtgcc ctgcgaggcg ccgggcggct gcagggcgag
      121 gtcgattgga ttcttgcgca tggttccggt cttccgcgtg gcgatgccgt cgaggtggag
      181 gcgatacgcc gggtcttcgg tccggccgcg gcgaatcccc gggtcagcag cgtcaagggg
      241 gccatcggcc atactctagg agctagcggc gccttgagcg ccgccatcgc agtgatggcg
      301 ttgacggaga gcagcattcc gcctacttcg ggcctgaccg atccagaccc acagttcgat
      361 ctcgacttcg tggtatcgcg gccgggactc ggtgcgctcg gcgtcgtggt cgtcaactcg
      421 ttcagcacct tgggccagca cgcctcgctc gtgctcacac gagcgtcatg aggcatacgg
      481 cgcaaggatc gagatgaaaa ggatcgagat gaagaggaga gtggcacgat ggacctaacg
      541 tggctgggcc acggctgcat ccgcatgcgg ggacgggaaa cgactatcct ctgcgatccg
      601 tgttcgaagg atacggggta caacatcggt cgccaggccg ccgacatcgt gaccctgagc
      661 cacgatcacc ccgcgcacag taatgtcgcc gtcgtcagcg gtcatccgaa ggttatcgcg
      721 ggaccagggg aatacgaggt cggcggagcg ctcatcatcg gcgtgcgcac gtatcacgat
      781 gcgcaatccg gtgcgttgag ggggaagaac acggcctacc tcatcgagat cgatggaaca
      841 cgggtttgcc acctgggcga cattgggcac ctgccgtccg agcagcagat cgagcaactc
      901 gtcggcgcag acatcctcgt ggtgcctgtc ggcggtggca ccacgatcga tgcgcaggtg
      961 gctgcgcgca ccgtgagttt gctcgagcca cggatcgtcg tgcccgtgca ctacgcgacg
     1021 ccggcttcgc gcgtcgaggg tctcgacgga gtggagcgct tcctgaagga gatgggtgtg
     1081 acggacgtga cgcccgagtc caagatcagc accgggcggg gcggcgtcag tggagagacg
     1141 aggaccgtcg tgctcgagta ccgagcctag ggcgctgaag ccgcccgacc ttcgcgcggc
     1201 gtcacgttcc tgcgggcaga tcgatcagct catggcaaga acacaagcgg gttcaggtgg
     1261 gcgccgcctt ggcgcacctc gaagtgcagg tggacaccgg tgctgtagcc ggtggtgccg
     1321 acaatcccga tcgcctcgcc tgcggatacc acttgcccga cggagacgct gatgctcgac
     1381 aggtgcgcgt acagggtctc ccagccgccg ccgtgggaga tgatcacgta gtagccgtag
     1441 ctgtagccgg gatcgccgcc cgcgaacgta acggttccgg ctgcagcggc cgctaccgga
     1501 cctgtcgggt tctggaatcc atcgatgtcg atgccgagtg ggtggcctgg gccgaagtag
     1561 ctcgagatgt ttcccgagaa cggccatacg aacgatggct ggccgcccgc ctcgacggct
     1621 ggggcctccg ttggtgacgg ctcgggtggt gggggctcgg cggcgaacgg gttcggcggc
     1681 tcggcttccg gaccgacaat tggcacttcc gcctcgccct cggctgccgg cgctccctcg
     1741 gatggtgcgg cagggtcggc ggcgaacgga ttgccttcgt cgaccggctc cggcgcggtc
     1801 tcggccggcg tttcctcagc ggcgggctcc ggtggggcga agcttctggt gccgccgact
     1861 acgagcaact cttgtccgat gcgaatgtcg tccggtgacg taaggccgtt gggcgcgaag
     1921 ccgacaatgg cctcgaccgt cgtgccgtat tgctcagcaa gcccggcgat ggtatcgccc
     1981 ggttgtacgg ggtggagcac gccgtccgcc agcgggatgc ggatgttctg gccgggctgg
     2041 agagtctctg cgccaacgag gtcgaggttg gcggcaagga tggtgttcga cagcaggccg
     2101 aactgcgctg ctatcgagtt cacagtgtcg ccggggagga cgatatagtt gaagtaagcg
     2161 ccctgttgac cggcggcgcc ggtgttctgc gcctcctgct caggggtcgt cgcgtcgacc
     2221 cgaatcggat cctcttccgc gggagcggga ggctcctctt cgatgggctg ttccggctcg
     2281 ggcgtcggct cttcgaccgg ctgttcttcg ggaacgaccg tctcctcctg gggagcctcg
     2341 gccgcctcaa cgtcgggcag gacatcgacg cgtgcccgca cgtcggtcgc agcctcgggg
     2401 gtcgatgttg cgatttcctg cagctccgtc tcgccctcca cggtctccgg cagggccggc
     2461 gcgacgagga cggcgtcctg ctcctcgagc gtcacgctcg gggcgggagc gacttcacgt
     2521 atctgggcgc cgggtgcaat gccggggagg gagatggcgg gcgcctccgc cagatgcggc
     2581 agaccgtcgc catcgaacgc gccgttggcc acgcgcccaa gcacgacccc tgcaattccg
     2641 agtgcgagcg ccacgagatg caggtacggg cgtcgcaacg ggtgcagcga agctggctcg
     2701 agcgccgtgt cggcgtcgtg cggactgtcg gggaagagga cgcggcgggg aatatcgccc
     2761 cgcgagccgc gtcggccagg cgcggatgag atagcctcag tctcccttct ggtctggccg
     2821 gcgttgatcc gcgccggacg agagggccaa cactgagccg gagctccggc cccggctcga
     2881 ttccgcgcca ttattgcggc ttgttctctc aagcgcaagt cggtgatagc ctgcggttgc
     2941 gtcacgggga gtatgtaaaa cgggagcgcg catctttgct gatagaggct tcggatgcgg
     3001 cttcgccccg aggatggccg gcgggaggtg ttgcacactt gaggctacgc cgcactgcgc
     3061 accggcaacc ttcgttcgat tagcacacct atgctagact gacgtacagc ccgggaattg
     3121 ggcgagcctt atttcctttc ggagtgcgat gtgacccagg aggcgacggc gaccgtcgac
     3181 atggagacca tcgtgtcgct gtgcaagcgg cgcggtttca tcttcccgag cgccgagatt
     3241 tacggcgg
//
LOCUS       DATMBB010000234         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_485660, whole genome shotgun sequence.
ACCESSION   DATMBB010000234 DATMBB010000000
VERSION     DATMBB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485660"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VNL92_05150"
     CDS             complement(<1..350)
                     /locus_tag="VNL92_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94132.1"
                     /translation="MSSTLRLIDVEVFKLRKRWMTWLLLSVLLVLIVVVYVLLWAVTE
                     TLTDTAGGRPIGLFGTTQLSDVRETLFLEQAVPFGLQMTQIVGTFLGVILASGSVGGE
                     YGWDTVRPFLTAVP"
     gene            complement(334..1278)
                     /locus_tag="VNL92_05155"
     CDS             complement(334..1278)
                     /locus_tag="VNL92_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94133.1"
                     /translation="MTDGPVLVTRELTKRFGERLAVDKLNLHLNAGEVYGLLGPNGSG
                     KSTTVAMILALVRPSSGQIEVFGLPLQSHRWEILRRVGAIVESPAFYPYLSGRDNLRI
                     YARAIGVISERQIDDSLDRVDLLERADDRYDEYSLGMKQRLGIASTLLRDPQLIILDE
                     PTNGLDPAGTIEIRALIPRLASEGRTVLVCSHLLYEVEQTCERVTIMQRGQQLTETTV
                     HDLLQQERGIRIRIDGDGAGEGVIAALRSVPWVRDIRREEDSYLRVMAPADRASEMNR
                     ILVEHGIYVAELTPVHPTLESVFLEFTGDGSKPDVIDA"
     gene            complement(1332..2366)
                     /locus_tag="VNL92_05160"
     CDS             complement(1332..2366)
                     /locus_tag="VNL92_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWO94134.1"
                     /translation="MSEREPSFVTRYRPAILDAMYRAFDEATSDQPRLPLYDALRYHL
                     GWLTEEGAPREGADGKLLRPALLLLSCEALGGDIERALPAAASAELIHNFTLLHDDIE
                     DRSELRHGRPTVWWRWGDALAINAGDGLWAIARIAMHRLSAVGNHPARVLAAMDELDR
                     ACLALCEGQDRDIRSEGDATIGTDDYLATVRGKTAALTAACAAIGAILAGTGDGSVVA
                     MREFGLHVGLAFQLRDDALGIWGSIGKAPAEDIHSRKLSYPVVYALARATPSDRARLV
                     ELYSEAPDDAGVDEAIRILERSGAREATEAAAHAAAERAVGALHGLALREPFAGELEA
                     LARYAVERTR"
     gene            complement(2371..>2590)
                     /locus_tag="VNL92_05165"
     CDS             complement(2371..>2590)
                     /locus_tag="VNL92_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HWO94135.1"
                     /translation="IAGTDAHRESNLGYCATAFERPVADMESLLAELRAGRFAPVVGI
                     GRGLCLPFDASSLTQLETMQRDASRRTG"
BASE COUNT          482 a          851 c          827 g          430 t
ORIGIN      
        1 ctcgggacgg cggtgagaaa tggccgcacg gtatcccatc catactcacc gcccacgctt
       61 cccgacgcga ggatgacgcc caagaacgtg ccgacgatct gcgtcatctg gaggccgaag
      121 ggcacagctt gctcgagaaa gagggtctcc cgcacgtcgg atagctgcgt agtgccgaaa
      181 aggccgatgg ggcggccgcc cgcagtatcg gtcagcgtct ccgtcacggc ccagagcagc
      241 acgtacacca cgacaatcag gacaaggagc acactgagca ggagccaggt catccaccgc
      301 ttgcgcagct tgaacacctc gacgtcgatg agcctaagcg tcgatgacat caggcttact
      361 cccatcgccg gtgaactcca ggaacacgct ctccagtgtt ggatgcaccg gtgtgagctc
      421 ggcaacgtag ataccgtgct cgaccaggat gcgattcatc tcgctcgcgc ggtccgcggg
      481 cgccataacg cgcaggtacg aatcctcctc gcgccgtatg tcccggaccc acggaacgga
      541 gcgcagggcc gcgataacac cttctcctgc gccatcccca tcgatccgta tccggatacc
      601 gcgctcctgc tggagcagat cgtgcaccgt ggtctccgtg agctgctgtc ctcgctgcat
      661 aatcgtgacg cgctcgcagg tttgttccac ctcgtacaag agatggctgc acacgagcac
      721 agtgcggccc tcagaggcaa gacggggaat aagggcgcgg atctcgatgg ttccggccgg
      781 atcgagccca tttgtgggct cgtcgagaat gatgagctga gggtcgcgca gcagcgtgga
      841 ggcaatgccg agccgctgct tcatgcccaa cgagtactcg tcgtagcgat catcggcgcg
      901 ctcgagcaga tcgacgcggt ccagtgaatc gtcgatctgc cgctcgctaa tgacgccgat
      961 ggcccgcgcg tagatgcgga ggttgtcgcg ccccgaaagg tagggataga aggcaggcga
     1021 ttcgacgatc gccccgaccc gacgcaggat ctcccatcgg tgcgactgga gcggcagccc
     1081 gaatacttcg atctgtccgg aactcgggcg caccagcgcg aggatcatcg cgactgttgt
     1141 gcttttcccc gacccattcg gtccgagaag cccgtacacc tcacctgcgt tcaggtgaag
     1201 attcagcttg tcgacggcca ggcgctcgcc aaagcgcttc gtcagctcac gtgtcacgag
     1261 gacaggcccg tccgtcatgc tgatcctaga ctacaaggac tcggccaaag attcgagggg
     1321 tggccgatcg ctcagcgggt gcgctccacg gcgtagcggg ccaacgcctc gagctcgccg
     1381 gcgaaaggct cccggagcgc caagccatgg agcgcgccca cggcccgctc cgccgcggcg
     1441 tgggcggccg cctccgtcgc ctcgcgtgct cccgagcgct cgaggatgcg tatcgcttcg
     1501 tcgacacccg cgtcgtcggg agcttcgctg tagagctcga caagacgcgc tcgatcggaa
     1561 ggcgtggctc gcgccagtgc ataaacgaca ggataggaca gcttcctgct atggatgtcc
     1621 tcggcaggag ccttcccgat gcttccccag atgccaagcg cgtcatcgcg gagttggaac
     1681 gccaagccga cgtggagacc gaactctcgc attgcgacga ctgatccgtc gccggtccct
     1741 gcgagaatgg cgcctatggc ggcgcaggcc gccgtgagtg ctgctgtctt cccccggacc
     1801 gtggccagat agtcgtccgt tccgatggtc gcatcccctt cggagcgaat gtcacggtcc
     1861 tgaccctcgc agagcgcgag gcaggcgcga tccaattcat ccatcgcggc gagcacgcgt
     1921 gcaggatgat tcccgacggc cgacagacga tgcatcgcga tgcgggcgat ggcccacaag
     1981 ccgtcgcccg cattgatggc gagcgcgtct ccccagcgcc accaaacggt cggccgtcca
     2041 tggcgcagct cgctcctgtc ctcgatgtcg tcgtggagca aggtgaagtt atggatgagc
     2101 tcggcgctgg cagcggcggg aagcgcccgc tcgatgtccc ccccgagcgc ctcgcagctc
     2161 agcagcagca gtgccgggcg aagcagcttt ccatcggcgc cctcgcgagg tgctccctcc
     2221 tcggtgagcc atccaaggtg gtagcgaaga gcatcgtaca gcggcagacg cggctggtcg
     2281 gacgtagctt cgtcgaaggc gcggtacatg gcatcgagga tcgccgggcg gtagcgggtt
     2341 acgaaggatg gctcacgctc ggacacgcga tcacccagtg cgacgcgagg cgtcccgctg
     2401 catcgtctcc agctgtgtca gggagctggc gtcgaagggg agacacaagc ccctcccaat
     2461 gcccacaacg ggagcgaatc ttccggcacg caactcggcg agaaggcttt ccatgtcggc
     2521 gacgggacgc tcgaaggccg tcgcgcagta tcccaagttg ctctcccgat gagcgtcggt
     2581 cccggcgatg
//
LOCUS       DATMBB010000235         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_438868, whole genome shotgun sequence.
ACCESSION   DATMBB010000235 DATMBB010000000
VERSION     DATMBB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438868"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..702
                     /locus_tag="VNL92_05170"
     CDS             58..702
                     /locus_tag="VNL92_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO94136.1"
                     /translation="MTQPGPTPFRPEHFRREDESPDEAFYSDPRLVVHIDDHAIEALR
                     GYLRETIAPGAEVLDLMSSWRSHLPDEIEYKRVVGLGMNDVELAENPQLTQRIVQNLN
                     VDPRLPFADAEFDVVLCTVSVQYLTRPVEVFAEVARVLRPNGRLVVSFSNRLFPSKAI
                     ALWRYSNDGDHAQIVMAYLRYAGGYEAIEFVDRSPRLPYSTDPMYIVTARRSTG"
     gene            complement(715..951)
                     /locus_tag="VNL92_05175"
     CDS             complement(715..951)
                     /locus_tag="VNL92_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWO94137.1"
                     /translation="MAVKAYVLIVAQPGKTKAVFESVRKAPGISECHEVMGPYDIVVE
                     VEAKELAEIPPILSERIRTIEGVESTTSLVTFPE"
     gene            complement(967..>2386)
                     /locus_tag="VNL92_05180"
     CDS             complement(967..>2386)
                     /locus_tag="VNL92_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HWO94138.1"
                     /translation="AVKLVAEEGVGTIAAGVAKAYADTILISGHDGGTGASPLSSIKY
                     AGSPWELGLAETQQVLLINGLRERVTLQTDGGLRTGRDVVVAALLGADEFGFGTAALV
                     AVGCKMARQCHLNTCPVGVATQRDDLRQKFIGQPEHVIRFLGGVAEEVREILAQLGFR
                     RLEEIIGRTDLLEQIPDERHPNAGLLDLSQLLAMPSVNGSPLHQTWARNDRPNDVPFD
                     DMLLNDVRDAIEFGTPVRRSYRIRNVDRTVGARVAGEIALRYGQQGLPPETIRLQFTG
                     AAGQSFGAFCTSGLVLTLIGEANDYVGKGMSGGLVAVAPPKDATFATHENTIAGNTLL
                     YGATGGELYIAGRAGERFAVRNSGAEAVVEGVGDHCCEYMTAGLVVVLGETGRNFGAG
                     MSNGLAFVLDERGDFPERYNDELIAIEKVTDPRDIDMLRYKIIRHLEVTGSGRAADVL
                     AQWDHYLPMFWKVEPKSMAKAQ"
BASE COUNT          388 a          785 c          775 g          438 t
ORIGIN      
        1 gagcaggact agcgtgggtg gctgcccgcc gtgtagcttg tgggcaggga gaacgggatg
       61 acgcagcccg gacccacacc gttccgaccc gagcacttcc ggcgggagga cgaatcaccc
      121 gacgaggcct tctactcgga cccgcgtctc gtcgtccaca tcgacgacca cgccatcgaa
      181 gctctgcgag ggtacctccg ggagacgatc gctcccgggg ccgaagtcct cgatctgatg
      241 agctcgtggc ggtcgcacct gccggacgag atcgagtaca aacgggtcgt cggattgggt
      301 atgaacgatg tggagctcgc cgagaaccct cagctcaccc agcggatcgt ccagaacctc
      361 aacgtggacc cgcggctccc cttcgccgac gcggaattcg acgtcgtcct ttgtacggtg
      421 tcggtgcagt accttacccg gcccgtcgag gtgttcgccg aggtggctcg cgtgctgcgc
      481 ccgaacggtc gcctcgtcgt cagcttctcg aaccggctgt tcccgagcaa ggcgatcgcg
      541 ctgtggcggt acagcaacga tggcgaccac gctcagatcg tgatggcgta cctgcgctac
      601 gcgggcggat acgaggcgat agagttcgtc gatcgatccc cgcggctgcc gtactccacc
      661 gatccgatgt acatcgtcac ggcgaggcga tcgaccggct agctcgggac gtagctactc
      721 ggggaacgta acgagggatg tcgtactctc gacgccttcg atggtccgga tgcgctcgct
      781 gaggatgggg ggaatctcgg cgagttcctt cgcttcgacc tccacgacga tgtcgtacgg
      841 ccccattacc tcgtgacatt cggagatgcc gggagccttg cgtacgctct cgaagacggc
      901 cttggtcttg ccgggctgcg ccacgatgag cacgtatgcc ttcacggcca tgcgatcgct
      961 cctgtcttac tgggccttcg ccatgctctt cggctcgacc ttccagaaca tgggcaggta
     1021 gtggtcccac tgtgcgagca cgtcggcggc cctgccgctg cccgtcacct cgaggtggcg
     1081 gatgatcttg tagcggagca tatcgatgtc gcgtggatcg gtgaccttct cgatcgcgat
     1141 gagctcgtcg ttgtagcgct ccgggaagtc cccccgttcg tccaggacga aagcgagccc
     1201 gttcgacatg ccagcgccga agttgcgccc ggtctcgccg agaacgacga cgaggcccgc
     1261 ggtcatgtat tcacagcagt ggtcgcccac gccttcgaca actgcctcgg cgccgctgtt
     1321 ccgcacggcg aaccgttcgc ccgcacggcc ggcgatatac aactctccgc ccgttgcgcc
     1381 gtagagcagc gtgttgccgg caatcgtgtt ctcgtgcgtg gcgaaagtgg cgtccttcgg
     1441 gggggccacg gccacgaggc cgccactcat gcccttgccg acataatcgt tcgcttcacc
     1501 gatgagcgtg agcacgagcc cggacgtgca gaacgcccca aaactttgcc ccgctgcacc
     1561 cgtgaactga agccggattg tctccggtgg gagaccctgt tggccgtacc gcaaggcgat
     1621 ttcgcccgcc acgcgcgcgc caaccgtccg atcgacattg cggatgcggt aggagcgccg
     1681 gaccggggtg ccgaactcga ttgcatcccg aacgtcgttc agcagcatgt cgtcgaaggg
     1741 tacatcgttc ggccggtcgt tgcgcgccca ggtctggtgg agggggctgc cgtttacgct
     1801 gggcatggcg aggagctggg acagatcgag gaggccggcg ttggggtgcc gctcgtccgg
     1861 gatctgctcc agcaggtcgg ttcgcccaat gatctcttcg aggcgccgaa agccaagctg
     1921 cgccaggatt tcgcgtacct cttccgccac accgccgagg aagcggatga cgtgctccgg
     1981 ctgtccgatg aacttctggc ggagatcgtc gcgttgcgtg gcgacgccga ccgggcaggt
     2041 attgagatgg cactgtcgtg ccatcttgca gccaacggcg acgagcgctg ccgttccgaa
     2101 cccgaactcg tccgcgccga gcagcgcggc gacgacgaca tcgcgtccgg ttcgcaatcc
     2161 gccgtccgtc tgcagcgtga cccgctcgcg cagcccgttg atcagcagca cctgctgcgt
     2221 ctcggcgagg ccgagctccc agggcgagcc ggcgtacttg atcgagctga gcggcgatgc
     2281 gcccgtccct ccatcgtgac ccgagatcag tatcgtatcg gcgtaggcct tggcgacgcc
     2341 cgccgcgatt gtgccgacgc cttcctcggc gacgagcttt acggcg
//
LOCUS       DATMBB010000236         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_162867, whole genome shotgun sequence.
ACCESSION   DATMBB010000236 DATMBB010000000
VERSION     DATMBB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162867"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VNL92_05185"
     CDS             complement(<1..277)
                     /locus_tag="VNL92_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94139.1"
                     /translation="MATVVIAAASALAVTQLTRGETDRQASAPMERRVVFVAGLQTSS
                     DCDDPPTDGNGTSWGGLWQAIASGLGLEPDDLFVYSYSGDYECDGAPP"
     gene            complement(325..1179)
                     /locus_tag="VNL92_05190"
     CDS             complement(325..1179)
                     /locus_tag="VNL92_05190"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HWO94140.1"
                     /translation="MKIGAHVSTSGGIANAPARAAQIGAETIQIFTSIPQTWRKRSIS
                     DAEVEAFRGSLAEHGIGPVFIHAIYLINLATSNPELLEKSVSALITDLEHGSRIGAGG
                     VIFHVGIFQTGSFDDVAPQIIDAIRRILDATPEDTRLLIENAAGERKGVGCQFSEIGA
                     LIRGVGSPRLGMCLDTAHAFASCYDVTDRQTLDRMMDELDREVGLERLTAVHANDSKV
                     ECGRGVDRHENIGEGKIGRAGFENILAHPAFRDLPLLLEVPGYDGKGPDARNVETLRS
                     LATAVGAA"
     gene            complement(1238..>2197)
                     /locus_tag="VNL92_05195"
     CDS             complement(1238..>2197)
                     /locus_tag="VNL92_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HWO94141.1"
                     /translation="TLREQPAAEALRALPQLPGGPQVLARLHAFLDRFGDRLAGGDDW
                     AEPTWREAPEQALQRAAALLETTSDGHVDHTQLIERRERITAEALCSIEDPDLRREFE
                     ESLRMMRRAWPLKEEHNFYIDQTLAAHMRYVMQEIGRRLEQRGAFDAPDDVFMLTLDE
                     ALDAFRAPQAPVLRSRVAERKAEREWQLSLDPPMLLGAPVEPEGAHGPPAAPRVLRGR
                     PGSPGRARGPARVVLSIDDFPKLRAGDILVCRSTTPHWTPLFQSAAGIVAEAGGALSH
                     AAIAAREYGIPAVLSVPAVTAILADGEPIEVDGAAGEVRRSID"
BASE COUNT          338 a          728 c          771 g          360 t
ORIGIN      
        1 ccggcggcgc accatcgcat tcgtagtcgc ccgagtagct gtagacgaac aggtcgtccg
       61 gctcgagccc gaggccggac gcgatcgcct gccacagccc gccccagctc gtcccattgc
      121 catctgtggg tggatcgtcg cagtccgatg aggtttgcag gccggcgacg aagaccaccc
      181 tgcgctccat gggcgccgat gcctgccggt cggtctcgcc ccgggtgagc tgcgtcaccg
      241 ccaaggcgga tgctgctgcg atgacgaccg tcgccaaggc gagggcccac ctggacgctg
      301 cagtacgggg gaggtgtggg cgaatcatgc agcaccgaca gcggtcgcga gggagcgcaa
      361 cgtctcgacg ttccgcgcat ccggcccctt accgtcgtag ccggggacct cgaggagcag
      421 cggcagatcg cggaaggcgg ggtgggccag gatgttctcg aagcccgccc ggccgatctt
      481 tccctcgccg atgttttcgt ggcggtccac cccgcggccg cactccacct tcgagtcgtt
      541 cgcgtgcacg gcggtgagcc gctcgaggcc gacctcacgg tccagctcgt ccatcatccg
      601 atcgagggtc tggcggtcgg tcacgtcata gcaggatgcg aaggcgtggg cagtatcgag
      661 acacatgccg agccgcgggc tgccgacgcc gcgaatgagg gctcctatct cggagaactg
      721 gcaaccaacg cccttccgct cgccggccgc gttctcgatc aggaggcggg tgtcctcggg
      781 tgtagcgtcg agaatgcgac gtatcgcgtc gatgatctgg ggcgccacgt catcgaaaga
      841 gccggtttgg aagattccga cgtggaagat gacgccgcct gccccgatgc gcgaaccgtg
      901 ctcgagatcg gtaatcagcg cgctgacgga cttctcgagc agttctgggt tcgaggtcgc
      961 gaggttgatg aggtagatcg cgtggatgaa gacggggccg ataccgtgct ccgcgaggct
     1021 gccccgaaac gcctcgacct ctgcgtcgct gatggagcgt ttccgccacg tttgcgggat
     1081 ggaggtgaag atctggatcg tttcggcgcc gatctgggcc gcgcgggcgg gtgcgttcgc
     1141 gataccgccc gaggtcgaga cgtgcgcacc gatcttcatg cgtgccctcc cgctatgctc
     1201 agttggctga ttatagacga atgccgtcgc ggatcgctca gtcgatcgag cggcgaacct
     1261 cgccggccgc gccgtcgacc tcgatcggct cgccatcggc gagtatcgcg gtgacggcgg
     1321 ggacgctgag caccgcaggg atgccgtact cgcgggcggc gatggccgca tgcgagagcg
     1381 cgcctcccgc ttcggccacg atacccgcgg cgctctggaa cagcggcgtc cagtggggcg
     1441 ttgtggaccg gcacacgagg atgtcgccgg cgcggagctt ggggaagtcg tcgatcgata
     1501 ggacgacccg cgcagggcca cgggcgcgcc caggtgagcc gggcctgccg cgcagcaccc
     1561 gcggcgccgc agggggaccg tgagctccct ccggttccac cggcgcgccg agcagcatcg
     1621 gcgggtcgag ggagagttgc cattcgcgct ccgccttccg ctcggcgaca cgggagcgga
     1681 gtaccggggc ctggggcgcg cgaaaggcgt cgagggcctc gtcgagcgta agcatgaaga
     1741 cgtcatcagg ggcgtcgaac gccccccgct gctccaggcg gcggccgatc tcctgcatga
     1801 catagcgcat gtgggcggcg agcgtttgat cgatgtagaa gttgtgctcc tccttgagcg
     1861 gccacgctcg ccgcatcata cgcagcgatt cctcgaactc gcgccgcaag tccgggtcct
     1921 cgatgctgca aagagcttcc gctgtgatcc tctcgcgccg ctcgatgagc tgggtgtgat
     1981 cgacgtgccc gtcgctcgtc gtctccaaca gggcagcagc tcgctgcagc gcctgctccg
     2041 gcgcctctcg ccacgtaggc tctgcccaat cgtccccgcc cgccagccta tccccgaagc
     2101 ggtcgaggaa cgcgtggagg cgggcgagaa cctgcggacc acctgggagc tgcgggagcg
     2161 ctctcagcgc ttccgctgca ggctgctcgc gcagcgt
//
LOCUS       DATMBB010000237         4550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_72020, whole genome shotgun sequence.
ACCESSION   DATMBB010000237 DATMBB010000000
VERSION     DATMBB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72020"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VNL92_05200"
     CDS             complement(<1..944)
                     /locus_tag="VNL92_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWO94142.1"
                     /translation="MTLSRPRPALQYAPVEGHLNLARVVLGDAAAHPRTADRPALTFI
                     DGRGDPTWTYRELWDWVSAIGRGLLASGLTPGERVLVRLAHSPAYAAAFFGAVAAGLV
                     PIPASPLLTESEARFLADDSEAAAVVTSPELRVGGFRGLEIFSDRLAALDGPGPLPDT
                     RAEEPAYLIYTSGTGARPKGVLHAHRSILGRLFMLEAWEGFGPGDRTLHAGTLNWSYT
                     LGVGLMDPWLVGAHAMLFGGSRDPAAWPAIIAERRISVFAAVPTVFRQILKYGRPEAA
                     DLSLLRHGLCAGEPLHPAILQEWRQRAGTDLYEALGMT"
     gene            complement(955..3138)
                     /locus_tag="VNL92_05205"
     CDS             complement(955..3138)
                     /locus_tag="VNL92_05205"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HWO94143.1"
                     /translation="MVMHPVPPERSEAAPPHRGGEPLDIDTIGDALVAKEGDVFLFTD
                     ESGEIRPDDRAGFGLYRGNTRYLSEYRLLLQGQAAIALLSTAANSFESEQVMTNRRLR
                     GKRGRIAAPQTLQVRRRRVVDGGLLERLELSNYGVEPLSIELTLRIAADFADIFEVRG
                     LEGVQRGRRQDTIVNRASVLFRYESLDGLSLSTELAFDPPPAEVTADHVRYVLELSPH
                     VTHVIEVAVRVDRRATPGLDYASARLHDEYREWRRRPARITVEGNETFAAAIGRAFDD
                     LRMLTRQTDGYALLGAGTPWYDDLFGRDSVVASLQVLPYRPEIARNTLRLLAKYQGQE
                     VVPEREEQPGKILHELRGGELARTGQVPFGRYYGSIDSTPLFLILYSEYLLWTGDDEL
                     AQELAPNVESALAWVDRYGDPDGDGYLEYQSSAEGGLVNQGWKDSHDGIIDENGAILD
                     APIALVEVQAYVYAALNGLARVAGKWSSRERRDVLMERAATLQRRFTRDFWLGRERFL
                     ALALDGAKRPSRAIASNAGHALWGGILQQAPGDVVARRLFSPELFSGWGIRTLATSSP
                     RYNPMGYHLGTVWPHDNSLIGMGLKRYGHDHLLIQLTSALFDAAQGFPYVRLPELFCG
                     NERAEHSPPVPYPVACRPQAWGAGTIPYFVQALLGLVPDASERTLFLVRPTLPPWLTS
                     LMIEDIHVGEGSGNLLIQRRFDRTEVKVIGADNLNVEVVSEWPRP"
     gene            complement(3163..4236)
                     /locus_tag="VNL92_05210"
     CDS             complement(3163..4236)
                     /locus_tag="VNL92_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWO94144.1"
                     /translation="MRIAVVAPLVESVPPKLYGGTERVVHLLTEELVRRGHDVTLFAS
                     GDSWTSARLVPCSEGSLRASGITDEVPATREMVRRVYGRAHEFDIIHCHVDWHPFPHA
                     RVCPVPTISTTHGRLDLPEIVREYEAFPEHPLVSISCAQQQYLPRANWLATVHNAVDV
                     ERFTFRGRGGDYLVFLGRFSPEKGPDRAIAIAMRVDMPLIMAAKIDPKDRAYYEAIVE
                     PLIRRHHPRVRYIGQVDERAKDELLGGAYAYLFPIDWPEPFGLTMVESMATGTPVLAI
                     GLGSVPEIVADGVTGFVCGSLDEMADAVKRVPEIDRAACRERVARLFSPQRMCDGYEE
                     VYAQLLATPSAPAPDALDGRMSA"
BASE COUNT          815 a         1496 c         1528 g          711 t
ORIGIN      
        1 tccgtcatgc ccagcgcttc atagaggtcc gtgccggcgc gttgccgcca ctcttgcagg
       61 atggctggat gcaacggctc gccggcacaa agtccgtgcc gcaacaacga caggtcggcc
      121 gcctcgggcc ggccgtactt caggatctgc cggaataccg tcggcacggc cgcgaaaacc
      181 gatatccgtc gctccgcgat gatggccggc cacgcggccg ggtctcgcga gccaccgaag
      241 agcatcgcgt gcgccccgac cagccacggg tccatcagtc cgactcccag cgtgtaggac
      301 cagttcaacg taccggcgtg caaggtgcgg tcgcccgggc cgaacccctc ccaggcttcg
      361 agcatgaaga gacgccccag aatcgagcga tgtgcgtgaa gcacgccttt tggccgggcc
      421 cccgttcccg atgtgtagat gaggtaggcg ggctcttcgg cgcgggtatc gggcagcggg
      481 cccggaccat cgagcgcagc caggcggtcg ctgaatatct cgagcccgcg gaacccgccc
      541 acgcgcagct ccggcgacgt gacgaccgcg gccgcttcgg agtcatcggc gagaaatcgc
      601 gcctcggatt cggtgaggag cggcgacgcg gggatcggca cgagaccggc cgccaccgca
      661 ccgaagaacg cggcggcata cgccggcgag tgggccagcc gcacgagcac ccgctcgccg
      721 ggcgtgaggc cgctcgccag caggccacgg ccgatggcgc tcacccagtc ccagagctct
      781 cgatacgtcc aagtaggatc ccctcgccca tcgatgaacg tgagggccgg gcggtcggcc
      841 gtgcgcgggt gcgcagcagc gtcgccgagg acgacgcgcg cgaggttgag atgcccctcc
      901 acgggagcgt actgcagcgc tggacgcgga cgcgatagag tcacggatac gggctcaagg
      961 gcgaggccat tcggacacga cttcgacgtt gaggttatca gcgccgatga ccttcacctc
     1021 ggttcggtcg aagcggcgct gaatcaagag attgcccgag ccttcgccga catggatatc
     1081 ctcgatcatc aggctcgtaa gccaaggcgg gagggtcggt cgcacgagga acaaggtacg
     1141 ctccgacgca tcgggcacca gcccaagcag cgcctgcacg aagtacggga tggtccccgc
     1201 accccatgcc tgcggccggc aggcgacggg gtagggaacg ggcggactgt gctcggcacg
     1261 ctcgtttccg cagaacagct cgggcaggcg aacgtaggga aagccctgtg ccgcatcgaa
     1321 gagggcgctc gtcagctgta tgagcaggtg atcgtgcccg taccgtttga ggcccatccc
     1381 tatgagggag ttgtcgtgtg gccagaccgt gccgaggtgg tagcccatcg ggttgtaccg
     1441 gggcgagctc gtcgcgagcg tgcggatgcc ccacccggag aacagctcgg gcgagaagag
     1501 ccggcgggcc acgacatcgc caggggcctg ctggaggatg cccccccaaa gagcgtggcc
     1561 cgcgttcgag gcgatggccc tcgaggggcg cttggcgcca tcgagggcta gcgccaagaa
     1621 tcgctcccgc cccagccaaa agtcgcgggt gaatcttcgt tggagtgtcg cggcacgttc
     1681 catgagcaca tcgcgccgct cccggctcga ccatttgccg gcgactctcg ccaggccatt
     1741 gagcgccgcg taaacgtagg cttgcacctc gacgagcgcg atcggcgcgt cgaggatggc
     1801 cccgttctcg tcgatgatgc catcatggga atccttccaa ccctggttga cgaggccacc
     1861 ctccgcgctc gactgatact cgaggtagcc gtcgccgtcc gggtcaccgt agcgatccac
     1921 ccaggcgagg gccgattcaa cgttaggggc gagctcctgc gccagctcat cgtcgccggt
     1981 ccagagcagg tactcgctgt acaggatgag gaacaacggc gtggaatcga tacttccgta
     2041 gtaccgtccg aagggcacct ggccggtcct cgccaactcg cctccccgga gctcgtggag
     2101 aattttgccg ggctgctcct cgcgttcggg cacaacctcc tgtccctggt acttggcgag
     2161 gagacgcagc gtgttgcggg cgatttccgg acggtacggc agtacctgga gggacgcgac
     2221 gacgctgtcc cggccgaaca agtcatcgta ccagggcgtg ccagcgccca gcagcgcgta
     2281 cccgtcggtc tgcctcgtga gcatacgtag atcgtcgaac gcccgcccga tcgccgccgc
     2341 gaacgtttca ttcccttcga ccgtgatgcg agccggcctc ctgcgccatt cacggtactc
     2401 gtcgtggaga cgagcggacg cgtagtcgag ccccggcgta gcgcggcgat cgacgcgaac
     2461 ggccacctcg atgacatggg taacgtgggg cgacagctcg agcacgtagc gtacgtgatc
     2521 ggcggtgact tccgccggcg gcggatcgaa ggcgagttcg gtcgaaagcg agaggccgtc
     2581 gagcgattcg taccgaaaca ggaccgaggc gcggttcact attgtgtcct gacggcggcc
     2641 gcgctgaaca ccctccaatc cgcgaacctc gaagatgtcg gcgaagtctg cggcgatgcg
     2701 cagggtcagc tcaatcgaga ggggctcgac cccgtagttc gaaagctcga gccgctcgag
     2761 caggcctccg tcgactacac gtcgccggcg aacctgcagc gtctggggcg cggcaatgcg
     2821 cccccgcttg ccgcgcaagc ggcggttcgt catcacttgc tccgactcga agctgttggc
     2881 cgccgtggag agcaacgcga tcgctgcctg gccttgcagg aggagccgat actccgagag
     2941 gtagcgcgtg ttgccgcggt agaggccgaa cccggcccga tcgtcgggtc gaatctcgcc
     3001 cgactcgtcc gtgaagagga atacgtcgcc ctctttggcg accagtgcat cgccaatcgt
     3061 gtcgatatcg agtggctcgc cgccgcggtg cgggggagca gcctcggatc tctcgggcgg
     3121 gaccggatgc atcaccatca gcgcgtctca cccgatgctc ggtcaggcac tcatccgccc
     3181 gtcaagcgcg tcgggagccg gagcgctggg tgtggccagg agctgtgcgt aaacctcctc
     3241 gtatccgtca cacattcgtt gtggcgagaa cagacgagcc acgcgctcgc gacatgcggc
     3301 acggtcgatc tcgggcacgc gcttcacggc gtcggccatc tcgtcgagcg agccgcacac
     3361 gaaccccgtc acaccgtccg ccacgatctc ggggacggag ccgagcccaa tagcgagcac
     3421 cggcgttccg gtggccatcg attcgaccat cgtgagtccg aacggctcgg gccaatcgat
     3481 ggggaagaga taggcgtacg ctccgcccag aagctcatcc ttggctcgct cgtcgacctg
     3541 accgatgtag cgcacccggg gatgatgtcg tcgaatcagc ggttcgacga tcgcctcgta
     3601 gtaggcccgg tcctttgggt cgatcttcgc cgccatgatg aggggcatat cgacacgcat
     3661 ggcgatggca atcgctcggt cgggtccctt ctcgggcgag aaccgcccca ggaacacgag
     3721 gtagtcgccg ccacggccgc ggaacgtgaa gcgctcgacg tcgaccgcgt tatgaacggt
     3781 cgccagccaa ttggctcggg gaagatactg ctgctgggca cagctgatcg agaccagcgg
     3841 gtgctccggg aacgcttcgt actcccggac gatttccggc aagtcgaggc gcccgtgggt
     3901 tgtcgaaatg gtgggcaccg ggcagacccg agcgtgggga aaagggtgcc aatcgacgtg
     3961 gcagtggatg atgtcgaact cgtgggcccg gccatagacg cggcggacca tctcccgggt
     4021 cgccggaacc tcgtcggtga tgccggaggc gcgtagcgac ccctccgagc acgggacgag
     4081 cctcgcggac gtccacgaat ccccactcgc gaacagcgtg acgtcgtgtc cgcgtcggac
     4141 gagctcctcg gtcagaaggt ggactacgcg ctccgtcccg ccatacaact tcggcgggac
     4201 ggattcaacg agaggcgcga cgacggcgat gcgcatgctg gcaactcctg caaggcttgc
     4261 tatgagcacg aacgagggca cacgcgccgg cgtttccgcc ggctgccatc cgggttcata
     4321 gtagcaacgc atcccgctca atcgctaggt cccaaggcga tagcggcaaa gcccagatcg
     4381 ggggattgac agtcctgggg cgcgacgcta aggtttgcga gcctgagggg cgcttctctg
     4441 ccgatcccgc actcgaacgg cccagttgat gcaacaacct cgattcgcga tcacgaacgc
     4501 cacgctcatc gatggcacgg ggcgccctcg gaccccgcga gcgaccgtcg
//
LOCUS       DATMBB010000238         3192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_93980, whole genome shotgun sequence.
ACCESSION   DATMBB010000238 DATMBB010000000
VERSION     DATMBB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93980"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VNL92_05215"
     CDS             <1..970
                     /locus_tag="VNL92_05215"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWO94145.1"
                     /translation="NDVFPAAMSISSAEECVRHLLPALRHLHEALASKSERFANIVKI
                     GRTHMMDATPLTLGQEFSGYAAQVENGVARVESVLPRVYELALGGTAVGTGLNTHPEY
                     ATRVAAEIARLTGLPFVSARNKFEALAAHDALVELSGQLKTVAASLMKIANDIRLLGS
                     GPRAGIGELLLPENEPGSSIMPGKVNPTQAEAMTMVCAQVMGNDVSVTIGGALGHLEL
                     NVFKPVIIYNTLQSIRLLADASMSFCDNMIAGLEPNIERIHGLMESSLMLVTALSPHI
                     GYDKAAKVAHKAHHEGTTLREAALALGYVTNEQFDAWVRPEDMTHP"
     gene            complement(1004..1735)
                     /locus_tag="VNL92_05220"
     CDS             complement(1004..1735)
                     /locus_tag="VNL92_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWO94146.1"
                     /translation="MVTLLVKRGEQGDLREVEYRIPAGTGWVVLDAIHYIQAHIAPDL
                     AVRWNCKAAKCGSCSAEVNGRPKLMCKTRLDDYDPSEPLTVSPMKTFPHIKDLVTDVS
                     WNYEVNKRIQPFTPREDADFIIHQEDMDRVQEFRKCIECYLCQDTCFVLRNHQYLDRY
                     AGPRYFVRVAGLEMHPMDAGNRLQFLKDEAGISYCNITKCCTEVCPEDIHITDNAIIP
                     LKERVADRYYDPVRWMFRKILRRKD"
     gene            complement(1816..>3192)
                     /locus_tag="VNL92_05225"
     CDS             complement(1816..>3192)
                     /locus_tag="VNL92_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWO94147.1"
                     /translation="GRILQRAFGGHTYRRLAHVGDRTGLEMIRTLQDRGVHQGLDVFM
                     ECTVARLFLDDGRIAGCLAYWRETGRFVVFKAKAIVMATGGIGKMFQITSNSWEYTGD
                     GMAMAYEVGAELLDMEFTQFHPTGMVWPPGVRGILVTEAVRGEGGVLTNALGERFMER
                     YDPKRMELSTRDIVAKAINEEVGAGRGSPHGGVFLDISHRGHDYIIRKLPSMYHQFKD
                     LADVDITREPMEVGPTMHYMMGGIRVDPETQASTVPGLYACGECAGGMHGGNRLGGNS
                     LSDLLVFGQRAGAAAAEYAKGTSQGSMDPDQLDATARELVAPFERERGETPYDLQAAL
                     QETMQTGVGIVRDAAGLSRAIDGIAGIAQRMGGMWIEGTRLYNPGWHLSWDMRQMLII
                     AEAVARAALQREESRGGHTRLDFPESDHGYWESVNSIVRKKSDGSMETTTMPKPEMPE
                     ELRALLAD"
BASE COUNT          537 a         1009 c         1042 g          604 t
ORIGIN      
        1 gaacgacgtg tttcccgcgg ccatgtcgat ctcgagcgcg gaggagtgcg tgcgtcatct
       61 cctgccggcc ctccgtcacc ttcacgaggc gctcgcgtcg aagtcggagc gattcgcgaa
      121 catcgtgaag atcggtcgga cccatatgat ggacgcgacg ccgctcacgc tcgggcagga
      181 gttttctggc tacgccgctc aggtggagaa cggcgtcgcg cgcgttgaga gcgtgctccc
      241 acgcgtctac gagctcgcgc tcgggggcac agccgtcggc acaggcctga acacgcaccc
      301 cgagtacgcg actcgggtag cggcggagat cgctcgcctg accggattgc cgttcgtctc
      361 ggcgcgcaac aagttcgagg ctctggcggc gcatgacgcg ctggtggagc tgtccggtca
      421 actgaagacc gtcgcggcct cgttgatgaa gatcgccaac gacattcgtc tgctcgggtc
      481 tggtccgcgc gccggcattg gggagctgct gctgccggag aatgagcccg gctcctcgat
      541 catgccgggt aaggtgaacc cgactcaggc cgaggcgatg acgatggtgt gcgcccaggt
      601 gatggggaac gacgtgagcg tgacgatcgg cggcgcgctg gggcacctcg agctcaacgt
      661 attcaagccg gtcatcatct acaacacgct gcagagcatc cgccttctcg ccgacgccag
      721 catgtcgttc tgcgacaaca tgattgctgg gctcgaaccg aacatcgagc ggatccacgg
      781 cctcatggag tcctcgctca tgctcgtgac ggcgctcagc ccgcatatcg gctacgacaa
      841 ggcggcgaag gtcgcgcaca aggcccatca cgaggggacg acgctgcgtg aggcggcgct
      901 ggctctcggg tacgtcacga acgagcagtt cgatgcgtgg gttcggcccg aggacatgac
      961 gcatccgtag tcccggggct ccgggtccgg agctggttgc ggttcagtcc ttcctgcgaa
     1021 ggatcttgcg gaacatccaa cggacaggat cgtagtagcg gtccgccacc cgctccttca
     1081 gcgggatgat ggcgttgtcc gtgatgtgga tgtcctccgg acacacttcg gtacagcact
     1141 tggtgatgtt gcagtaggag atgccggcct catccttgag gaactggagg cggttgccgg
     1201 catccatcgg gtgcatctcg agcccagcaa ctcggacgaa gtaccgaggg cccgcgtagc
     1261 ggtcgaggta ctgatggtta cgcaggacga agcaggtgtc ttggcacagg tagcattcga
     1321 tgcacttgcg gaactcttgg acgcggtcca tatcctcctg gtgaatgatg aagtccgcat
     1381 cctcgcgggg agtgaacggc tggatgcgct tgttgacctc gtagttccag gagacgtccg
     1441 tgacgaggtc cttgatgtgc gggaaggtct tcatcggcga aacggtgaga ggttcgctcg
     1501 gatcgtagtc atcgaggcgt gtcttgcaca tcagcttcgg acgtccgttg acctcggcgc
     1561 tgcacgagcc gcatttcgcg gccttgcagt tccagcgcac cgcgaggtca ggagcgatgt
     1621 gcgcctggat gtaatgaatc gcgtcgagga cgacccatcc ggttccggcg ggaatgcggt
     1681 actcgacctc tcgcagatcc ccctgctcgc cgcgcttgac cagcagggtc accatctggc
     1741 cagtcgtctc agcggcttgg gatgctgcgg tggcttgtgg tgtcgtgggt gcttgcgcca
     1801 tacgtcgctc ctcagtcagt cggcaagaag ggcccggagc tcttcaggca tctccggttt
     1861 cggcatggtg gtcgtctcca tggagccgtc gctcttctta cggacaatgc tgttgaccga
     1921 ttcccaataa ccgtggtcgg attcggggaa atcgagcctg gtgtgaccac cgcggctctc
     1981 ttcgcgctga agcgcggcgc gcgcgacggc ctccgcgata atgagcattt gccgcatgtc
     2041 ccagctaagg tgccaccccg gattgtagag gcgcgtgcct tcgatccaca tgccacccat
     2101 gcgctgggcg atgccggcga tgccgtcaat cgcgcggctc aggccggcag cgtcccggac
     2161 gatgcccacg cctgtctgca tggtctcctg cagcgcagct tgcagatcgt agggtgtttc
     2221 gccacgctcg cgctcgaagg gcgcgacgag ctcgcgtgcc gtcgcgtcga gctgatccgg
     2281 gtccatgctc ccctgcgaag tgcccttggc gtactccgcc gcagccgcgc ccgctcgttg
     2341 gccgaagacg agcagatcgg acagcgagtt gccgccgagc cgattgccgc cgtgcatgcc
     2401 gccggcgcac tcgccgcacg cgtacagccc gggaaccgtc gatgcctggg tctcggggtc
     2461 gacgcgaatg ccgcccatca tgtagtgcat cgtcgggccg acttccatcg gctcgcgtgt
     2521 aatgtcgaca tcggcgaggt ccttgaactg atggtacatg ctgggcagct tgcgaatgat
     2581 gtagtcgtgc cctcgatgcg agatatcaag gaacacgccg ccatgcggac ttcccctgcc
     2641 ggcccccacc tcctcgttga tcgccttggc gacgatgtcg cgggtggaca gctccatgcg
     2701 cttcgggtcg taacgctcca tgaagcgctc gcccaaggcg ttcgtgagta cgccgccttc
     2761 gccgcgcacc gcctcggtca cgaggatgcc gcgaactccc ggtggccaga ccatcccggt
     2821 agggtggaac tgggtgaact ccatatcgag gagctcggcg ccgacctcgt aggccatggc
     2881 catgccgtcg ccggtgtatt cccacgagtt tgacgtgatc tggaacatct tgccgatgcc
     2941 gcccgtcgcc atgacgatcg ccttggcctt gaacacgacg aatcggccag tctctcgcca
     3001 ataggcgagg cagccggcga tccgcccgtc gtcgaggaag agccgcgcga cggtgcactc
     3061 catgaacacg tcgagcccct ggtggacgcc gcgatcctgc agggtgcgga tcatctccaa
     3121 ccccgtgcgg tcgcccacat gcgccaaacg acgatacgtg tggccgccga aagcgcgctg
     3181 cagaatccgt cc
//
LOCUS       DATMBB010000239         2596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_200704, whole genome shotgun sequence.
ACCESSION   DATMBB010000239 DATMBB010000000
VERSION     DATMBB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200704"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1156..2211)
                     /locus_tag="VNL92_05230"
     CDS             complement(1156..2211)
                     /locus_tag="VNL92_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO94148.1"
                     /translation="MRWPGIGVTSLGLLVGGGIVGREVIRRRRAMDLSGKVVLVTGSS
                     RGLGFVLAQEFARRGSSLVMCARNESVLREAQREVESLGAEVLAEPCDVGNPAQVEKL
                     VAAAVDRFGRIDVVVNNAGVITMSPFEMLDRADFEEAMDSMFWGVYNTTSAVLPEMRR
                     RKEGTIVNITSIGGKVAAPHLLPYTSAKSAAVGFSEGLHAEVARDGVRVVTVVPGFMR
                     TGSHVNARFKGKHRWEYAWFSLLATLPLTSTDAQRAARRIVMATRLGETEVIVTPQAQ
                     LAARLHGAMPGLVVDIASAVGRLLPSTEGNDITKQPALGKESETPVSASFLTSLGRRA
                     AERFNQNSSPQPSQLPE"
     gene            complement(2228..2506)
                     /locus_tag="VNL92_05235"
     CDS             complement(2228..2506)
                     /locus_tag="VNL92_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94149.1"
                     /translation="MTGKAALLHVAIGVAGGAAFGALPGRGAAPLLVGPAYGLAFYLV
                     AIGLAGPSLGILPPMHRQSRTFLVRELLTHLVFGTTVASSVARAEQRR"
BASE COUNT          462 a          868 c          778 g          488 t
ORIGIN      
        1 catcccatgc agtggtcgaa tgatacatcg gaatgccccg aacgctgttg tcgtcagggg
       61 cactttgaag ggggcgtgac ttatttgcct gggtctctac tctccgccac ttaggtcggc
      121 ctcgagcgcg tcagtccgcg cgacgtttcg cattacgacg ggctacgtga cgcccgacgg
      181 ctccgtgggg ccgcccacag cctggatgcc tggcggttgt cgcccgggcc tgtcgcggtt
      241 tacgtgagga gtaccaccaa gaggacaacc accaggatca gaacaatcgt cccaactccg
      301 atatacataa tgactccctc tcactgccca cccggggccg tttcgcctca gcctaccggg
      361 gattacgtat cgtgggagtg aaggggaagt attggccgct tgcgatactg tcgcgcttta
      421 tgcgaggttc acgcctttcg gaagcagaac attgaccgct tcataccttc tcgaagcggt
      481 cgttgttctc ttcacctgag ccgggggtat gggtgggggg atgctgcaac gctcgccgta
      541 gtcagctgta tccgtatgag gtacgggacg ccggcgcaca ctagcggttg cggcgcttgc
      601 aggaagggag gcgcgtgtcg ccctcgtccc cttctcgttc agcctcagcg gggaaaggag
      661 gcgggcagcc ttgcgctcgc agtctcacga agcgtaggcg gctcatggca tgcgcgagag
      721 cggtggccca agaccatctg cacgacgagc acgtcgtctg ctagatccga ggaaacggag
      781 ggcacttgtt gcggccggtc ttcggagtga cgatgacgaa ggggaaggct cggccggcgc
      841 cctccccttc gtgattctac agaccgagta ccgcgagcag ggcgaagatg gtatcgacgg
      901 tgctggtcac gattggcaga agcccttcga gcagatgcgt caccatgatt cacgcctcct
      961 tggttcactt ccgtgcctat ggtgaagagc ttgtgcaccc tcgcccgagt gagtcgatcg
     1021 acaccttcgt atcgtgagct agttgagtta cgagttgatt gcccctcggt tacgcatttg
     1081 tgaacggatt ccgcgttcga gcctgattca cgaggctctg cccgagctcg ccaacgtctg
     1141 cgctcccgac gcggttcatt caggaagctg gcttggttgc ggagacgagt tctgattgaa
     1201 gcgctccgcc gcgcggcgtc cgagcgacgt caggaacgac gccgaaaccg gcgtctcgct
     1261 ctcctttccc aaggccggct gcttcgttat gtcattgccc tcggtcgaag gcaacagccg
     1321 tcccacggcc gacgcgatgt cgacgacgag gcccggcatc gcgccgtgca gtcgtgccgc
     1381 aagctgcgcc tggggcgtaa cgattacctc cgtctcgccg aggcgagttg ccattacgat
     1441 ccgtcgcgcc gccctttgcg catcggtcga agtcaacgga agcgtggcca gcagactgaa
     1501 ccatgcatac tcccatcgat gcttcccctt gaatcgtgcg ttgacatgcg atccggttcg
     1561 catgaaccct gggacgaccg tcacgacacg aacaccgtcg cgcgcgacct ccgcatgcag
     1621 cccctcggag aaccccaccg cggccgactt ggcactcgtg tacgggagga ggtgcggcgc
     1681 cgcgaccttg cctcctatcg acgttatgtt aacgatcgtc ccttccttcc gccgcctcat
     1741 ttccgggaga acggcgctcg tcgtgttata gacgccccag aacatgctgt ccatcgcctc
     1801 ttcgaaatcg gccctgtcga gcatctcgaa cggggacatg gtgatgacgc ctgcgttatt
     1861 caccacgacg tctatccgcc cgaaccggtc cacggccgcg gcgaccagct tctcaacctg
     1921 ggccgggttg cccacatcgc atggctcagc aagcacctct gcccccaggg actcgacctc
     1981 ccgttgggcc tcgcgcagca ccgactcgtt acgcgcgcac atgacgaggg acgacccacg
     2041 gcgagcgaac tcctgcgcca gcacgaatcc caggccccgc gagcttcccg tgacgagcac
     2101 gaccttgccg gaaaggtcca tcgctctcct tcgccggatc acctcccgcc cgacgattcc
     2161 acccccaaca agaagtccca ggctcgtcac tccgatcccg ggccacctca ttggtacgcc
     2221 tcctcgctta gcgccgctgt tccgcacgcg ccacactact cgccactgtc gttccgaaga
     2281 cgaggtgtgt gagaagctcg cgcactagga aggttctgct ctgccggtgc atcggcggca
     2341 ggatgcccag ggacggcccg gcgagaccga tagcaacgag gtaaaaggcg agtccgtacg
     2401 cgggtcccac aagcagcgga gcggcgccac ggcccggcag tgcaccgaac gccgcgcctc
     2461 ccgccacgcc aatcgcgaca tggaggaggg ccgccttacc cgtcacggaa cttcgcctga
     2521 ggattggagg ccctcgccgc tcgacggccc atgccatctg gcgaggcggc gtatcggacc
     2581 cagtcccagc ctcctc
//
LOCUS       DATMBB010000240         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_147539, whole genome shotgun sequence.
ACCESSION   DATMBB010000240 DATMBB010000000
VERSION     DATMBB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147539"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..867
                     /locus_tag="VNL92_05240"
     CDS             220..867
                     /locus_tag="VNL92_05240"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWO94150.1"
                     /translation="MARISLPDKEARRLLGGGIVVLVTTAWHGQHNVAPIIWHTPLSL
                     EPPLIGIAVHPSRHTHDMIRYSQQFAINVPTPALMKHVQYTGVVTGADTNKIEAAHLP
                     THKAEKVEAPLLDYCIGWIECGVNDALRLGDHTLFIAEVASVTVEEEAFDGVWSLTPA
                     DQRPVHYVGGPFYAMLERRIEVTLGRSEPEPGEEQGAYLPPEETDEERRRESREA"
     gene            complement(864..1913)
                     /locus_tag="VNL92_05245"
     CDS             complement(864..1913)
                     /locus_tag="VNL92_05245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWO94151.1"
                     /translation="MRVESGFRTGDLATVPAEARRLERLGYDAANTPETQHNPFLPLV
                     LAAEHTERLHLGTAVAIAFARSPMVVANTFWDLQAYSRGRMSIGLGSQVKGHNIRRFS
                     VPWSPPAPRMREYVLALRAIFDTFQNGVPLDFQGQHYQFTLMTPNFNPGPIDHPRIPI
                     YISAVGKVMARVAGEVCDGVRIHGFTTRRYTEEVLFPAFEEGRRRSGRSLADFDISGG
                     GFIVSGRTQAEVEQAREATRRQVAFYASTRTYQAVLELHGWADVCTQLHEMVAQGRWS
                     ELHTLITDDILDEFAVFGTYDEIVPKVQARYEGLCDSIAFGPTPRTAGEEDRIRETIA
                     GLKTIRTRRDAVQAA"
     gene            2080..>2266
                     /locus_tag="VNL92_05250"
     CDS             2080..>2266
                     /locus_tag="VNL92_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015408062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HWO94152.1"
                     /translation="MAQLSARQRAILDFIADFRRTKDYPPSIRDIQQGCDISSTSVVD
                     YNLKALERLGYIRRDREV"
BASE COUNT          420 a          770 c          710 g          366 t
ORIGIN      
        1 cgacgacgcg tccttcgatg agggcgggga gcgcctgggc ggaagcggcg cgagcatcgg
       61 gaagcatcag cacgcccaga aacgcgagaa gcgcgaggat cagacgccgc ggtgcagccc
      121 attcgcccat cggcgtccag cttagcacac gaacgctttc ccactcgcgc cggcgtgcgc
      181 tatcgtagac gaacccgaac cgcagcccga ggaggtcgcg tggccaggat aagcttgccc
      241 gacaaggagg cacgccgtct gcttggcgga ggcatcgtcg tgctggtgac cacagcttgg
      301 cacggccagc acaacgttgc ccccatcatc tggcacacgc cgctttcgct cgaaccgccg
      361 ctcatcggca tcgccgtgca cccctcacgc catacgcacg acatgattcg ctacagtcag
      421 cagttcgcca tcaacgtgcc cacgccagcc ctcatgaagc acgtgcagta caccggcgtc
      481 gtcacgggcg cggacacgaa caagatcgag gccgcgcatc tgcccacgca taaggcggag
      541 aaggtggagg cgcccctcct cgactactgc attggatgga tcgagtgcgg cgtgaacgat
      601 gccttgcgcc ttggcgacca cacgctgttc atcgcggaag tcgcgtccgt gaccgtggaa
      661 gaggaggcgt tcgacggcgt gtggtcactg acaccggcag accagcgtcc cgtgcactac
      721 gttggtgggc cgttctacgc catgctcgag cggcgcatcg aggtgacgct cggccgcagc
      781 gaaccggagc cgggcgagga acaaggcgcc tatctccccc ccgaggagac agacgaagag
      841 cgccgtcgcg aaagccggga ggcctaagcg gcctgcaccg cgtcacgccg cgtgcgaatc
      901 gtcttcaagc cggcgatcgt ctcgcggatc cgatcctcct cgcctgctgt gcgtggcgtc
      961 gggccgaaag cgatggaatc gcaaaggccc tcgtaccgcg cctgcacctt cggcacaatc
     1021 tcgtcgtacg tgccgaacac cgcgaactcg tccaggatgt cgtcggtgat cagcgtatgc
     1081 agctcgctcc accggccctg cgccaccatc tcgtgcagct gagtgcagac atcggcccat
     1141 ccgtgcagct cgagcaccgc ctggtacgtc cgggtcgagg cgtagaaggc gacttgccgg
     1201 cgggtggcct cgcgcgcctg ctccacctcc gcttgggtcc ggcccgagac gatgaacccg
     1261 ccgcccgaga tgtcgaagtc cgccaggctg cgcccgctac ggcgcctccc ctcctcgaag
     1321 gctgggaaca gcacctcctc cgtgtagcgg cgcgtcgtga acccgtggat gcgcacacca
     1381 tcgcacacct cgccggctac gcgagccatc accttgccga cagcggagat gtagatggga
     1441 atgcgcgggt gatcgatcgg tccggggttg aagttggggg tcatgagcgt gaactggtag
     1501 tgctgcccct ggaagtcgag cggcactcca ttctggaacg tatcgaagat cgcacgaaga
     1561 gcgaggacat attcgcgcat gcgaggcgcc ggagggctcc acggcacgct gaatcgccga
     1621 atgttgtggc ccttcacctg gctcccgaga ccgatgctca tcctgccgcg cgagtacgcc
     1681 tgcaggtccc agaacgtgtt cgccaccacc atgggactgc gcgcaaacgc tatcgcgacg
     1741 gcggtcccga ggtgcaggcg ttccgtgtgc tcggccgcca gcacgagggg cagaaacgga
     1801 ttgtgctgcg tctccggtgt gttggcagcg tcgtagccga gacgttcgag ccggcgtgcc
     1861 tccgccggaa ccgtcgcaag atcgccagtg cggaaccccg attcgactcg cattgcgcgc
     1921 ctcccccttc cctgagctga acctcgtgag cgcatcctag cagatcgtcc cagcgcgcgg
     1981 ttgccgcgtt cttacattcg ccttgacgga agtgaaatgc acgttctaaa ctcgcacaga
     2041 gcgaacacac tttctattcc tagccacagg aggccgcaga tggcgcaact ctccgcccgg
     2101 cagcgagcga tcctcgactt catcgccgac tttcgtcgaa cgaaggacta ccccccgagc
     2161 atccgggaca tccagcaggg gtgcgacatc agctcgacgt cggtcgtcga ttacaacctc
     2221 aaggcgctcg aacgcctcgg gtacattcgg cgcgaccgcg aggtat
//
LOCUS       DATMBB010000241         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_121757, whole genome shotgun sequence.
ACCESSION   DATMBB010000241 DATMBB010000000
VERSION     DATMBB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121757"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VNL92_05255"
     CDS             complement(<1..657)
                     /locus_tag="VNL92_05255"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HWO94153.1"
                     /translation="MTLRRLIICCDGTWNTPDRTDGGIGVPTNVVRMARAIPPVAPDG
                     TPQIVYYDQGVGTDNMINKYTGGAFGIGLSRNVRDAYLFLIHNYIEGDELYFFGFSRG
                     AYTVRSTAGMIRKIGLLDKVEADRAMEAWRIYREHAGGPDSQRAIAFREAFSRYPVRI
                     RFLGVWDTVGALGIPGAHMDVGGGHRDHRLADAALIWIADGARAAGLALNDAYMEQIA
                     D"
     gene            777..923
                     /locus_tag="VNL92_05260"
     CDS             777..923
                     /locus_tag="VNL92_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWO94154.1"
                     /translation="MAKDPVCGMDVQENQAAATSQYQGQTYYFCSTSCKTNFDRDPQK
                     YVKQ"
     gene            1007..1270
                     /locus_tag="VNL92_05265"
     CDS             1007..1270
                     /locus_tag="VNL92_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWO94155.1"
                     /translation="MKASKPEALRRLAYIEGHVKGIRRMVENDEYCVDILKQTRAVKR
                     ALDRFDALLLEGHFAGCVAEGFREGRDDDVLRELHELFDLSRR"
     gene            1324..>2567
                     /locus_tag="VNL92_05270"
     CDS             1324..>2567
                     /locus_tag="VNL92_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWO94156.1"
                     /translation="MGLDQIVVTLGGIVLVAALAWYFFGPKAARQAELRGGAQEVRVT
                     VKGGYSPNYIRVQQGVPLRLIFDRQEGGECTSRVVFPDFGLNRSLPAFATTTVEFTPE
                     KSGTFGFACGMNMVHGTLLVEPSENGASHNGSAPASAAAPPASDGHAHETALAVGMGT
                     QHDMGETAEVEFAVEGGGVSCPTCVTNIESILKDIHGVERVDANLGAGRVSVAFVPGE
                     VSVDDLRQAIESSGYRVRQRPQPGSTATEDSEAAARRAEIRDLSRRLLVGAVLTAPVL
                     FAVMVGDIFDPGWMPGLLMEDWFQLLLIVPVYVYTGWPIHRIGWLTLLHRTSDMNTLV
                     TIGTTTAFVYSLVVTVAPDALPEDLRDVYYEAVGVILVLIVLGRLLEARAKAGTGEAI
                     RKLIGLQARTARVVRDGREQEIP"
BASE COUNT          461 a          825 c          847 g          434 t
ORIGIN      
        1 gtccgcgatc tgctccatat acgcgtcgtt gagggcgagg cccgccgctc gggcgccatc
       61 ggcgatccag atgagcgcgg cgtcggcgag ccgatgatcg cgatggcctc ctccgacgtc
      121 catgtgcgcg ccggggatgc ccaacgcccc caccgtatcc caaacgccga gaaaccggat
      181 gcgcacggga tagcgcgaaa aggcctcgcg gaaggctatc gcccgctggg agtccgggcc
      241 tccggcgtgc tcacgataga tgcgccaggc ctccatcgcg cggtcggctt cgaccttgtc
      301 gagcagaccg atcttgcgaa tcatgccggc cgtgctgcgg acggtgtagg cgccgcggct
      361 gaacccaaag aagtagagct cgtcgccctc gatatagttg tggataagga agagataggc
      421 atcgcgcaca ttgcgcgaga ggccaatgcc gaacgcgccg cccgtgtact tgttgatcat
      481 attgtcggtt cccacgcctt ggtcgtagta cacgatctga ggcgtgccgt ccggcgcaac
      541 gggcgggatg gcgcgagcca tgcgcaccac gttggtcggc acgccgatcc caccatcggt
      601 ccggtcgggc gtgttccatg tgccatcgca acagatgatg agccggcgca acgtcatccc
      661 cctgacgaat ggcgcctatc gtagcacctg tcagcctcac gtgatacgac gacatgacac
      721 gtcgacgaca ttgtcacgct cgctgggtgt aggctggaga aagaaggagg tgcgctatgg
      781 ccaaggatcc ggtttgcggg atggacgtgc aggagaacca ggcagcggcg acgagtcaat
      841 accagggaca gacctactac ttctgctcga catcctgcaa gacaaacttc gatcgcgacc
      901 cacagaagta cgtcaagcag taggggagtc gtaggcgctt gacggcgtcc ccacatcgtc
      961 tatactgacc ttacccccac caggggggta gggtcaggag gcgatcgtga aggctagcaa
     1021 accggaggcg ctgcgcaggc tggcctacat cgagggccac gtaaagggca tccgcaggat
     1081 ggtcgagaac gacgagtact gcgtcgacat cctgaagcag acacgcgccg tgaagcgcgc
     1141 cctcgatcgc ttcgatgcac tgcttctcga ggggcatttc gcgggctgcg tcgccgaggg
     1201 cttccgcgag ggacgcgacg atgacgtcct gcgggagttg cacgagctct tcgacctctc
     1261 caggcgctag acggtacgtt cgcccgaccc ggcctcgcgc acgcaatcca tagaaggtga
     1321 tccatgggac tcgatcagat agtcgtcaca ctgggcggaa tcgtcctcgt cgccgcgctc
     1381 gcatggtact tcttcggccc gaaggccgcc cgccaggcgg agcttcgagg cggagcccag
     1441 gaggtgcgcg tcaccgtcaa gggtggctac tcgccgaact acatccgagt gcagcagggt
     1501 gttccactgc ggctcatctt cgaccgacag gagggcggcg agtgcacctc gcgcgtcgtg
     1561 ttccccgatt tcggcctgaa ccgcagtctg cccgcgttcg cgacaacgac cgtcgagttc
     1621 acgcctgaga aaagcggcac gttcggcttc gcgtgcggca tgaacatggt ccacggtacc
     1681 ctgctcgtcg aaccgtccga gaacggcgcc tcgcacaacg gctccgcgcc cgcgagtgcc
     1741 gccgcgccac ccgcgtccga tggacatgca cacgagaccg cgctcgccgt cggcatgggg
     1801 acgcagcacg acatgggcga gacagcagag gtcgagttcg cggtcgaggg cggcggagtc
     1861 tcctgtccga cgtgcgtgac gaacatcgag tcgatcctca aggacatcca cggtgtcgaa
     1921 cgcgtcgatg cgaacctcgg agctggccgc gtgagcgtgg ccttcgttcc cggggaggtg
     1981 agcgtcgacg atctgcggca ggcgattgag tcgtcgggct accgggttcg ccagcgccct
     2041 cagcccggct cgaccgcgac agaggacagc gaagcggcag cccgccgggc cgagatccgc
     2101 gatctctcgc ggcgtctcct cgtcggcgct gtgctcaccg cgcccgttct cttcgcggtc
     2161 atggtgggcg acattttcga cccgggctgg atgccgggac tcctcatgga ggactggttc
     2221 cagctgttgc tcatcgtgcc ggtgtacgtg tacacaggct ggccgatcca tcgcatcggc
     2281 tggctcacgc tcctgcaccg cacgtccgac atgaacacgc tggtgaccat cggtacgacg
     2341 acggcattcg tgtatagcct cgtcgtcacg gtcgcgccgg acgcgctccc cgaggatctg
     2401 cgtgacgtgt actacgaggc ggtgggcgtc atcctcgtcc tcatcgttct cgggcggctc
     2461 ctcgaggctc gcgccaaggc ggggacgggc gaggcgattc gaaagctcat tggcctgcaa
     2521 gcgcgcaccg cccgcgtggt tcgggacggc agggagcagg agatacc
//
LOCUS       DATMBB010000242         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_122185, whole genome shotgun sequence.
ACCESSION   DATMBB010000242 DATMBB010000000
VERSION     DATMBB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122185"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1877)
                     /gene="ligA"
                     /locus_tag="VNL92_05275"
     CDS             complement(<1..1877)
                     /gene="ligA"
                     /locus_tag="VNL92_05275"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWO94157.1"
                     /translation="MTTSERTLYAARERIEQLRAQIVYHDYRYFVLDSPEISDAESDE
                     LMRELRALEEAHPELITPDSPTQRVGGEPVAAFGIVEHPVPLLSLANAFKPEDLQNWY
                     RRVRQLAERDEVPLVAEPKIDGLAVALVYEEGRFVQGATRGDGRRGENVTANLRTIRS
                     LPLTLRSKNIPRRFEVRGEVYLSEQAFARINEERARVGQPLFANPRNCAAGSLRQLDP
                     RITASRPLDIWLYGLGWAEGDAPRTHWETLHWLQDLGFRVNPHVARYETIEGAIAHSR
                     EWETRREQLDYDIDGIVIKVDDFDIYDRLGVVGREPRWAIAFKFPPTQATTKLLAIEV
                     NVGRTGSLNPFAVLEPVVVGGARVKLATLHNEDDIRRKDIRVGDTVIVQRAGEVIPQV
                     VGPVLSKRTGAERVYSMPKQCPSCGAKVVRLEGEAMSYCPNRACPAQIFRGLVHFASK
                     GAMDIDGVGEALCAALLKQGLVRDPADLYSLTKQQLLTLERMGERSADNVLRAIEASK
                     DRPPSRLLFALGVRHVGSEVAEALLSHFGSIDALMEATEDELAGIPGVGRVIAQSVRA
                     YFQEPRNRELLEKLRRAGVRMAGAEPPRREGPLAGKTFVLTGSLERFPRSRATQIIES
                     LG"
     gene            complement(1905..>2234)
                     /locus_tag="VNL92_05280"
     CDS             complement(1905..>2234)
                     /locus_tag="VNL92_05280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWO94158.1"
                     /translation="RLPVTDQLRNGPRASLHGGVLAAVVDAAIASALATLLPEDTEAG
                     ALTATLDLNITYISAVREGELRAEGRILRRGRSIAFGQTDIFDATGELVSTGRATYRI
                     RGTAAGG"
BASE COUNT          361 a          743 c          748 g          382 t
ORIGIN      
        1 cccaggctct cgatgatctg cgtcgcccgc gaccggggga agcgctccag cgaaccggtg
       61 agcacgaagg tcttccctgc caacggccct tcgcgacggg ggggctctgc cccggccatg
      121 cgaacgccgg ctctacgcag cttctcgagc agctcgcgat tgcgcggctc ttgaaagtac
      181 gctcgaacgc tctgagcgat aacccggccg acaccgggga tgcccgcgag ctcgtcctcg
      241 gtcgcctcca tgagcgcgtc gatggagccg aagtgcgaga gaagcgcttc ggccacctcg
      301 ctccccacgt ggcggacgcc gagcgcgaag aggagacggc tcggaggcct atccttgctg
      361 gcctcgatgg cccgcagaac gttgtcggcg ctcctctcgc ccatccgctc gagcgtgagg
      421 agttgctgct tcgtcaggct gtagagatcg gccggatcgc ggaccaggcc ctgcttcagc
      481 agtgccgcgc agagtgcctc ccccacgcca tcgatgtcca tcgccccttt cgaggcaaag
      541 tgaacgaggc cgcggaagat ttgtgcagga caggcacgat tcgggcagta ggacatcgcc
      601 tcgccctcga gtcgcacgac cttagcgccg caggatgggc attgcttcgg catcgagtag
      661 acgcgttcgg cgccggtgcg cttgctcaac accggcccca ccacttgggg gatgacctcg
      721 cccgcgcgtt ggacgatgac cgtatctccg acgcgaatgt ccttacggcg aatgtcatcc
      781 tcgttgtgca gcgtggcgag cttgacgcgc gccccgccga cgacgacggg ctccagcacg
      841 gcgaacggat tcaggctgcc cgtgcggccc acgttcacct cgatcgcgag gagcttggtt
      901 gtggcctggg taggcgggaa cttgaaggcg atggcccagc gcggctccct gcccaccaca
      961 ccgagccgat cgtagatgtc gaagtcgtcg accttgatga cgatgccatc gatgtcgtaa
     1021 tccagctgct cgcgtctcgt ctcccattcc cggctatgtg cgatggcgcc ctcgatcgtc
     1081 tcatagcggg caacgtgcgg gttcactcga aagccgaggt cctgaagcca gtggagcgtt
     1141 tcccagtggg tccgaggagc gtcgccctcg gcccagccga ggccatagag ccagatgtcg
     1201 agcggacgcg acgcggttat ccgcgggtcg agctggcgga gggagccggc agcgcagttg
     1261 cgcggattgg cgaacaaagg ctgaccgacg cgggcccgct cctcgttgat gcgcgcgaac
     1321 gcctgctcgg acagatacac ctcgccccgg acctcgaagc ggcggggaat gttcttggag
     1381 cggagcgtca gtgggaggga gcgaatggtt ctcaggttgg ccgtcacgtt ctcgccccgg
     1441 cgtccgtcgc ctcgggtggc gccctgcacg aagcgcccct cctcgtacac gagcgccacg
     1501 gccagaccat cgatcttcgg ctccgcgacc agaggtacct cgtcccgctc cgccagttgc
     1561 cgcacccgcc gataccaatt ttgcaggtcc tcgggcttga acgcgttcgc caggctcagc
     1621 agcgggaccg ggtgctcgac gatgccgaac gcggcgacgg gctctccgcc gacacgctgc
     1681 gtgggagaat cgggcgtgat gagctccggg tgcgcctcct cgagcgcgcg aagctccctc
     1741 atgagctcgt cggactcagc gtcggagatt tcggggctat cgagaacgaa gtatcggtag
     1801 tcgtggtaga cgatctgagc gcggagctgc tcgattcgct ctctcgcagc gtagagggtg
     1861 cgttcgctcg tcgtcatggt gctagcggga gtatagcatc ggcttcagcc gccggcggcc
     1921 gtgccgcgaa tgcgatacgt tgcccggccc gtggacacca attcgccggt cgcgtcgaag
     1981 atgtcggtct ggccgaaggc gatggaccgt ccccggcgca ggattcgtcc ttcggcgcgg
     2041 agctcaccct cccgaactgc cgagatgtag gtgatattga ggtcgagcgt ggccgtcagc
     2101 gccccggcct cggtgtcctc cggcagcagg gtcgccagcg ccgaggcgat ggccgcatcg
     2161 accaccgcgg ccaggacgcc cccatgcaga gatgcacgcg gtccatttcg gagctggtcc
     2221 gtcacaggca gacg
//
LOCUS       DATMBB010000243         3020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_224327, whole genome shotgun sequence.
ACCESSION   DATMBB010000243 DATMBB010000000
VERSION     DATMBB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224327"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VNL92_05285"
     CDS             complement(<1..974)
                     /locus_tag="VNL92_05285"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWO94159.1"
                     /translation="MVTSSATTGLALETAMRQFDEAADILELDEGLRGVLAHPKLELT
                     TNFPVEMDDGSIRVFTGTRIQHNVARGPGKGGVRYHPDVTVDEVKALAMWMTWKCAVV
                     DLPFGGAKGGVCVDPRALSLNELENLTRRYTAEIGPLIGPERDIPAPDMGTNAQVMAW
                     MMDTYSMNVGYSVRGVVTGKPLSVGGTVGREAATGRGILHIARSYAERMGFALAGRRV
                     AVHGFGNVGAWAASFLAEEGCHIVAISDSSGGVYNPGGIEVAAARAAKRQGPLAQTQG
                     LGDHITNEELLALPVDILVLAAMEGAIDAKNASNVRAPLIIEGANGPI"
     gene            1117..2442
                     /locus_tag="VNL92_05290"
     CDS             1117..2442
                     /locus_tag="VNL92_05290"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWO94160.1"
                     /translation="MEEQKQYVLQTAKEHGIKFIRLWFTDILGALKSFAITVSELEGA
                     LEEGMRFDGSSIEGFARVDESDMRALPDPTTFQILPWRPTEQGVARMFCDIVQPDGSP
                     FEGDPRHVLKRTLRRASELGYTFYVEPEIEFFYFRGPDSPEPLDQGGYFDLTPLDAGT
                     DLRRETVLTLEQMGIPVEFSHHEVAPSQHEIDLRYTDALTMADSAMTYRLVVKEVAMR
                     NGVYASFMPKPLEGENGSGMHIYMSLFRGDDNVFFDQDDPLNLSPLARSFIAGLLHQA
                     REITLVTNQWVNSYKRLTPGYEAPVYATWGRHNRSDLVRVPDYKRGHEGSTRIEYRAP
                     DSACNPYLTFAVLLAAGLRGIEREYPAPQPVARDVFSMSAEELRDLKIDRLPGSLYEA
                     IAAAEDSDFLRETLGEHVHQTLIVNKKVEWEAYRNHISTYELRRYLPIL"
     gene            complement(2507..>3020)
                     /locus_tag="VNL92_05295"
     CDS             complement(2507..>3020)
                     /locus_tag="VNL92_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HWO94161.1"
                     /translation="FTCIIAIYNQTGKENISDFAGVAETSPFLALVLTASLFSLAGLP
                     LFAGFVTKFILFQAAANEGYLWLAAIGVFNSFVSLYYYLLVIKQMYVVAPPTPGRFRV
                     SPPMYGAAAILAIAVMAFGLYPRPFLEAADEATQDVFSASARNLEESDCAQPDDGLVQ
                     AGAPAPSPPC"
BASE COUNT          538 a          951 c          973 g          558 t
ORIGIN      
        1 ctgatgggcc cattcgcgcc ttcgatgatc aagggcgccc ggacattcga ggcgttcttg
       61 gcatcgatcg ctccctccat ggccgcgagg acgaggatat ccacggggag ggcgagcagc
      121 tcctcgttcg taatgtggtc acccagtccc tgcgtttggg ccagtgggcc ttgtcgcttc
      181 gccgcccgcg ccgcggcgac ctcgatgccc cctggattgt agacgccgcc cgaggagtcg
      241 ctgatggcga cgatatggca cccttcctcg gcgaggaacg atgccgccca ggcccccacg
      301 ttcccgaacc cgtgcaccgc aacgcgcctt ccggccagtg cgaagcccat ccgctcggcg
      361 tacgaccgcg cgatgtgcag gatcccgcgg ccggttgctg cctcgcgtcc gacggtgcca
      421 cccacgctca gaggcttgcc ggtgacgaca ccccgcacgg aatagccaac gttcatgctg
      481 taggtgtcca tcatccaggc catgacctga gcattggtgc ccatgtcggg agccggaatg
      541 tcccgctccg gtccgatgag cggaccgatt tccgcggtgt agcgacgtgt gaggttctcg
      601 agctcgttga gcgacagtgc tcgcggatcg acgcagacgc cgcccttcgc gccgccgaac
      661 ggcaggtcga cgacggcgca cttccacgtc atccacatcg cgagcgcctt gacctcgtcg
      721 accgtgacgt ccgggtggta acggacgccg cccttccccg gcccgcgtgc gacattgtgc
      781 tgaatgcgcg tgccggtgaa gacgcggatc gagccatcgt ccatctcgac agggaagttt
      841 gtcgtcaact cgagcttggg gtgagccaga acgccgcgca gcccctcgtc gagctcgagg
      901 atgtcggcgg cctcgtcgaa ttggcgcatc gcggtctcga gggcgaggcc ggtagtcgcc
      961 gaagatgtga ccatgaaccg tctccattcg agtggggatc tgagcaaaac gtaacacatt
     1021 ggtaactcta gccgcctcgc cgctcatgga taatgcttct gttcgccggg cgtgatacgc
     1081 tcgctcagag cagcctgatt caggaggccg tgcccgatgg aggagcagaa gcagtacgtc
     1141 ctgcagacag caaaagagca cgggatcaag tttatccgcc tttggttcac cgatatcctc
     1201 ggtgcgctca agagctttgc catcacggtc agcgagctcg aaggggccct cgaggagggt
     1261 atgcgcttcg atggctcgtc gatcgagggg tttgcgcgcg tcgacgaaag cgacatgcgg
     1321 gcactgccgg acccgaccac gttccagatc ctgccctggc gccccactga gcagggggtg
     1381 gcccgcatgt tctgcgatat cgtgcagccc gatggctcgc ctttcgaggg cgacccgcgt
     1441 catgtactga agcgcacgct tcgccgtgcg tctgagctcg gctacacctt ctacgtggag
     1501 cccgagatcg agttcttcta cttccgtggt ccggactcgc ccgagccgct cgatcaggga
     1561 ggctacttcg atctgacgcc gctcgacgct gggacggacc tgcgccgcga gaccgtgctg
     1621 accctcgagc agatgggcat tccggtcgag ttcagccatc acgaggtcgc gccctctcag
     1681 cacgagatcg atcttcgcta taccgatgcg ctgacgatgg cggacagcgc catgacgtac
     1741 cgcctcgtcg tgaaggaagt ggcgatgcgc aacggtgtgt acgccagctt catgccgaag
     1801 ccgctcgaag gtgaaaacgg cagtggtatg cacatctaca tgtcgctgtt tcggggcgac
     1861 gacaacgtgt ttttcgacca ggacgatccg ctcaacctgt ccccgctagc gcggtccttc
     1921 atcgctggtc tgctgcatca ggcgcgggag atcacgctgg tcaccaatca atgggtgaac
     1981 tcgtacaagc ggctgacccc gggttacgag gcgcccgtgt atgctacgtg gggtcgtcac
     2041 aaccggtcgg acttggtgcg agttccggat tacaagcgcg gccatgaagg gtcgactcgt
     2101 atcgaatatc gggccccaga ctccgcttgt aacccctacc tcacgttcgc agtattgctc
     2161 gctgccgggc ttcggggcat cgagcgggag tatcccgccc cacaacctgt tgctcgtgat
     2221 gtcttcagca tgtcggcgga ggagttgcgc gacctaaaga tcgatcggtt gccggggtcg
     2281 ctgtacgagg cgatcgctgc cgccgaggac agcgatttcc tgcgggaaac cctcggcgag
     2341 cacgtgcacc agacgctcat cgtgaacaag aaggtcgagt gggaggccta tcgcaaccac
     2401 atctcgacct acgagttgcg tcgctacctg cccatcctct gatcgttcga acggacgcgc
     2461 gcccctatcg tcgcccgcta cgccgttccg ccggatcgaa tagggctcaa cagggcggtg
     2521 atggcgctgg agcgccggcc tgcacgaggc cgtcgtccgg ttgggcgcag tccgactcct
     2581 caaggttccg tgcgctcgcg ctgaacacgt cttgggtcgc ttcgtccgct gcttcgagga
     2641 atgggcgggg gtagaggccg aacgccatga cggcgatggc caggattgca gcagctccgt
     2701 acatcggtgg cgagacgcgg aagcggccgg gcgtgggggg cgccacgacg tacatctgct
     2761 tgatgacgag caggtaatag tagagcgata cgaagctgtt gaacacgccg atcgccgcca
     2821 gccagaggta gccctcgttc gcggcggcct ggaagaggat gaacttcgtg acgaagcccg
     2881 cgaataacgg cagccccgcg agcgagaaca gggacgccgt gagcacgagg gccaggaagg
     2941 ggctcgtctc ggcaacgccg gcgaaatcgg agatgttctc tttgcccgtc tggttgtaga
     3001 tcgcgatgat gcaggtgaac
//
LOCUS       DATMBB010000244         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_66729, whole genome shotgun sequence.
ACCESSION   DATMBB010000244 DATMBB010000000
VERSION     DATMBB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66729"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2375
                     /locus_tag="VNL92_05300"
     CDS             <1..2375
                     /locus_tag="VNL92_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HWO94162.1"
                     /translation="EDRDYMYRTYASDPEARINLGIRRRLAPLLGNHRRKMELMNGLL
                     LALPGTPVIYYGDELGMGDNIYLGDRDGVRTPMQWSSDRNAGFSRANPQRLYLPVIID
                     PEYHYETVNVEAQQANPHSLLWWMKRIIALRKRHKAFGRGSIEFLYPENARILAFIRE
                     YEDEHILVVANLSRFVQAVELDLTRFRGATPIEMFGRTHLPPVGEAPYFLTLGPHAFY
                     WFILQARTDSIMAEPNAAGRTIPTLTTSGSWTTVFRGRAVSNLTALLPEYLRGRRWFG
                     GKARTIQTVHVVDAVPIGGRAGREPKAFLALLSVEYREGEPETYMLPLGLARGERSEA
                     LMQEAPQAIVARIESRTDGGTGILFDASLDEEFGETLLSAIARGRSFEGTGKLTASPT
                     PALRRIRSRADGPLCPTVLRAEQSNTSIVYGDKLILKLFRRPERGINPDLELGRFITE
                     RTKMRHVPQVAGYIEYRAGRSEPMTLAILQAYVPNEGDAWRYTIDELDRYFERVLALG
                     TLPDERLLPRQPMALLAASPTPSAAEDAIGAYLESARLLGQRTAELHLALASSPEDKN
                     LAPEPFTPFYQRSLFQSMRNLTAQMLSLLRRSIDRLPEAAREEGLRIAELEPVIIERF
                     QRINQSRLNITRIRCHGDYHLGQVLYTGNDFVIIDFEGEPARPLSERRLKRSPLRDVA
                     GMLRSFHYAAYTALLEADGAVRDEDRPMLESWARVWHTWVSATFLRAYLETAAGASFL
                     PADSGDLVTLLDAFIMEKAVYELGYELNNRPEWVRLPLQGILQLLEEGSS"
     gene            2375..>2985
                     /locus_tag="VNL92_05305"
     CDS             2375..>2985
                     /locus_tag="VNL92_05305"
                     /inference="COORDINATES: protein motif:HMM:NF045011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWO94163.1"
                     /translation="MPTSIIPRALRQLAADFDVQTAYTDVGGQRRQASIEALVAVLQA
                     LGAQLTGPDDAAEALRERRRELLSRAVPPVVVARVGEAAAVELHTRAQASGSLDAQLT
                     LESGEERQWSIVLGAAHPLRTLSLEDEDWVSRRIMLPEALPPGYHWLHIEGSGTRGDT
                     LVVSAPPTTYVPARSEDRIWGVFLPVYALQSNESWGSGDFTDLR"
BASE COUNT          522 a         1041 c          894 g          528 t
ORIGIN      
        1 acgaagaccg cgactacatg tatcgcacct acgcctccga tcctgaagct cgcatcaatc
       61 tgggcatcag gcgaaggctt gcccctctgc tcggcaacca tcggcgcaag atggagctga
      121 tgaacggcct gctcttggcg ctgccgggta cgccggtcat ctactacgga gacgagctcg
      181 gcatgggcga caacatctat ctcggcgacc gcgatggtgt ccgcacgccc atgcagtggt
      241 cttccgaccg caacgccgga ttctcgcgtg ccaatccaca gcggctctat cttcccgtca
      301 tcatcgaccc ggaatatcac tacgaaaccg tcaatgtgga agcccagcag gcgaaccctc
      361 actccctcct gtggtggatg aagcggatca tcgccctgcg gaagcgtcat aaggccttcg
      421 gccgcggctc catcgaattc ctgtacccgg agaacgcccg catcctcgcc ttcatccgag
      481 agtacgagga cgagcacatc ctcgtcgtcg ccaacctgtc ccgcttcgtc caggccgtcg
      541 agctcgacct cacccggttt cgcggcgcga cgccgatcga gatgttcggt cgcactcacc
      601 tgccacctgt cggcgaggct ccgtacttcc tcacgctggg gccacacgcc ttttactggt
      661 tcatcctgca ggcgcgcacc gatagcatca tggcggaacc gaacgctgcc ggacgaacga
      721 ttccaacgct cacgacgtcc ggctcctgga cgacggtgtt ccgtggacgg gccgtctcca
      781 acctcacggc cttgctcccg gagtatctcc gcgggcgccg gtggtttggc ggaaaggcac
      841 gcacgatcca gaccgttcac gtcgtcgatg ccgtgcccat tgggggtcgc gcggggcgcg
      901 aaccgaaggc gttcctcgcg ctcctcagcg tcgagtaccg ggaaggtgaa ccggaaacgt
      961 acatgctccc cttgggactg gctcgcggcg agcgttccga ggcgctgatg caggaggcgc
     1021 ctcaggccat tgtcgcccgc atcgagagcc gcacggatgg cggcacgggc atcctcttcg
     1081 acgcgtctct cgacgaggag ttcggcgaga cgctgctcag cgctattgca cggggacgct
     1141 cgttcgaggg cactggcaag ctcaccgcga gcccgacgcc cgccctccga cgcatccgct
     1201 ctcgcgccga tggcccactc tgccccaccg tgctccgcgc agagcagagc aacacgtcga
     1261 tcgtctacgg cgacaagctg atcctgaagc tctttcgccg gcccgaacgc ggaatcaacc
     1321 ccgatttgga gttgggtcgg ttcatcaccg agcgaacgaa gatgcgtcac gtcccacagg
     1381 tggccggcta tatcgaatac agggccggtc gaagcgagcc gatgaccctg gcgattctgc
     1441 aagcctacgt tccgaacgaa ggcgatgctt ggcgctacac gatcgacgag ctagatcgct
     1501 acttcgagcg cgtcctcgcg ctggggacgc tccccgacga gcgcctgctg ccgcgacagc
     1561 ccatggccct gcttgccgcg agcccaactc cctccgctgc cgaggacgcg atcggcgcct
     1621 acttggaatc ggcgcgcctg cttggacaac gcactgcgga gctgcacctc gccctcgcct
     1681 cctccccgga agacaagaac ctcgcgccgg agccgttcac tcccttctat caacgctcgc
     1741 tatttcaatc catgcgcaac ctgacggccc aaatgctgag tctcctgcgg cgcagcatcg
     1801 accgcctacc cgaggcagcg cgcgaggagg ggcttcgcat cgctgagctt gaacccgtga
     1861 tcatcgagcg cttccagcgc atcaaccaat ccaggctcaa catcactcgt atccgctgtc
     1921 acggcgacta ccacctcggg caggtgctgt acacgggtaa cgacttcgtc atcatcgact
     1981 tcgagggaga gcccgccagg ccgctcagcg agcgccgcct gaagcggtcc ccgctgcgcg
     2041 acgtggcggg catgcttcga tccttccact atgctgccta taccgcgctg ctcgaagccg
     2101 acggcgccgt tcgtgacgag gatcgcccga tgctcgagtc atgggcacgc gtctggcata
     2161 cgtgggtaag cgccaccttc cttcgtgcct atctggagac ggcagcaggc gcctcgttcc
     2221 tccccgcaga ttccggcgac ctcgtcacgc tcctcgatgc gttcatcatg gagaaggcgg
     2281 tatacgagct cgggtacgag ctgaacaatc gtcccgagtg ggtgcggctg ccgctccagg
     2341 gcatccttca gttgctcgag gaaggctcat cctgatgcca accagcatca ttccccgggc
     2401 tttgcgccag ctcgcggcag acttcgatgt tcagacggcg tacaccgatg tcggcggaca
     2461 acggcggcaa gcctccatcg aagcgctcgt agccgtgctt caggccctcg gcgcgcagct
     2521 cacgggacca gacgatgcgg ccgaggcgct gcgcgagcgg cggcgggaat tgctctcacg
     2581 tgccgtcccg cccgttgtcg tggcgcgtgt aggcgaagct gcggcagtcg agctacacac
     2641 ccgcgcccaa gcctccggct cgttggacgc gcagctcacg cttgaatcgg gcgaggagcg
     2701 gcaatggagc attgtactcg gggccgccca tccgctccgg accctttcac tcgaggacga
     2761 ggactgggta tcgcggagga tcatgctgcc ggaggccctg ccgccgggtt atcactggct
     2821 ccacatcgaa ggttccggca cccgcggcga taccctcgtc gtcagcgcgc cgccgaccac
     2881 atacgtgccc gcacggtccg aagatcggat ttggggggtc ttccttcccg tgtatgcgct
     2941 ccaatccaac gagagctggg ggtcgggcga tttcaccgac ctgcg
//
LOCUS       DATMBB010000245         2504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_285945, whole genome shotgun sequence.
ACCESSION   DATMBB010000245 DATMBB010000000
VERSION     DATMBB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285945"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VNL92_05310"
     CDS             <1..605
                     /locus_tag="VNL92_05310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWO94164.1"
                     /translation="VRGLHRFYEELALRFAEEGINAVAIDYFGRTAGVAKRGDDFDFM
                     DHVRQTKIATISEDTRAALDYLRSPEGGSCRALFTVGFCFGGSNSWLQAAQGHGLAGA
                     IGFYGRPGTGLTGEPGPIDSVAKFECPILGLMGGADPMIPEDQVNEYRQALDKAGVKN
                     EIISYPGAPHSFFDRAYEQFQEASADAWQRVLAFVRANAG"
     gene            640..1929
                     /locus_tag="VNL92_05315"
     CDS             640..1929
                     /locus_tag="VNL92_05315"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94165.1"
                     /translation="MGTGPLSGIRVLEFSVIYAAPFAGMNLADLGADVIKVEAEGGEA
                     FRHQGAVVPGHGKTFMWLNRGKRGLCLNLRDPRGQEAIHRLVATTDVVLINYRPGVPQ
                     RLRIDYETLSAIRPDLVYADIAGFGKQGPLAGDAASDIVAQAYGGAIALDAKLDDDGA
                     PVSIGVAIADLSTGLAIAMGISAALYHRAMTGEGQYLSAALVRTVMALSGVSVLREPV
                     HDAVLREPLIATMNRVRESGGSYAELIQSRTAGRGLLGAAFTLYYGGYRVKDGGVVLG
                     ALTPANRDAFRRVLGIEDDDSDTPDYDARDPENIAKALRLRDRIRALMKTKTVAEWMA
                     LFREAGAPAGPVNFPEELADDPQAATLMVDLEHELTGWQKQVAPVVDMSRTPTAIPRG
                     SPGLGQHTDEILQSIGYTPDEIRALHTDRVAFGPAVD"
     gene            complement(1943..>2504)
                     /locus_tag="VNL92_05320"
     CDS             complement(1943..>2504)
                     /locus_tag="VNL92_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWO94166.1"
                     /translation="ESEGLFADLTLSGEVDDGEDALDWLLARPEVDSTRLGVAGHSFG
                     GLVSVLLAARRPEDVRAIAPMSAVFDAPARFRRNFAAREPEWRARQAIALGPGGPMLK
                     VSFLDDLAGWDVREHARRVRAPALIVHGTADESVPTSDAAELASFLRPELVSVHLIEG
                     ADHVYSDAGALREVQGLVAEWFSDRL"
BASE COUNT          437 a          859 c          809 g          399 t
ORIGIN      
        1 atgtccgcgg ccttcaccgc ttctatgagg agctcgcgct cagattcgcg gaggagggca
       61 tcaacgccgt tgccatcgac tacttcgggc gaacagccgg tgtcgccaag cgcggcgatg
      121 acttcgattt catggaccac gtgcgccaga cgaagatcgc cacgataagt gaggacacgc
      181 gcgcggccct cgattacctc cgttcacccg aaggcggttc atgccgggcg ctcttcacgg
      241 tcgggttctg cttcggcggg tcgaattcgt ggttgcaggc cgcgcagggt catgggcttg
      301 cgggcgcgat agggttctac ggccggcccg gcacgggact caccggcgag ccgggaccca
      361 tcgacagcgt cgcaaagttc gaatgcccca tcctcgggct gatgggcggc gccgacccga
      421 tgatccccga ggatcaggtg aacgagtacc ggcaggctct cgacaaggcg ggagtcaaga
      481 acgagatcat cagctacccc ggtgcgccac acagcttctt cgatcgagcc tacgagcaat
      541 tccaggaagc ttcggccgac gcttggcagc gcgtcctcgc cttcgtgcgg gcgaacgcgg
      601 gctagcctca ctgcgccatc tcgtcatctg ggaggctcga tggggacggg accgctaagc
      661 ggcattcgcg tgctcgaatt ctcggtgatc tacgcggcgc ccttcgccgg gatgaatctg
      721 gccgacctcg gggcggacgt catcaaggtg gaggccgaag ggggcgaagc gtttcgtcac
      781 caaggtgcgg tcgtgccggg ccacggaaag acattcatgt ggctgaaccg aggcaagcgc
      841 ggcctctgcc tcaaccttcg tgacccgcgc ggccaagagg ccatccatcg cctcgttgcc
      901 acgacggacg tcgtcctcat caactaccgc cccggcgtcc cccagcgctt gcgcatcgac
      961 tacgagacgc tgtcggcgat tcggccggac ttggtctatg ccgatatcgc aggcttcggc
     1021 aagcaggggc cgctcgcggg cgatgccgcc tccgacatcg ttgcgcaagc gtacggcggc
     1081 gccatcgcgc tcgacgccaa gctcgacgat gacggcgcgc cggtttccat cggcgttgcc
     1141 attgctgatc tttcgaccgg cttggcaatc gccatgggca tatctgcggc cctctatcac
     1201 cgtgcgatga cgggagaagg tcaatacctc tccgctgccc tcgtccgcac cgtcatggcg
     1261 ctgagcgggg tcagcgtcct ccgcgagccc gttcacgatg cagtcctgcg cgagcccctc
     1321 atcgcgacca tgaaccgcgt gcgagagtcc ggcggctcct acgccgagct gatccagtcc
     1381 cgaacggccg gacgcggtct cctcggcgcc gccttcacgc tgtactacgg cggctatcgc
     1441 gtcaaggatg gtggagtcgt cctcggcgca ctgacgccgg ctaatcgcga cgcatttcgc
     1501 cgcgtcctcg gcatcgagga cgacgatagc gacacgccgg attacgacgc acgggatccg
     1561 gagaacatcg cgaaggccct tcgcctgcga gaccgcattc gtgccctgat gaaaacgaag
     1621 accgtcgcgg aatggatggc gctgttccgc gaagccggag cgccggcggg tccagtcaac
     1681 tttccagaag agctggccga cgacccgcag gcggccacgc tcatggtcga cctcgagcac
     1741 gagctaaccg gctggcagaa gcaggtggcg cctgtcgtcg acatgagccg caccccgacg
     1801 gccatccctc gaggctcgcc gggcctcgga cagcacaccg acgagatcct gcaatcgatc
     1861 ggctacacgc cggacgagat tcgcgccctc cataccgatc gcgtggcctt cgggcccgcg
     1921 gtcgactaag cgcgagcgcc tgctacagcc ggtcgctgaa ccactcggcc acgagacctt
     1981 gcacttctcg cagcgccccg gcgtcgctgt agacgtggtc ggcgccctcg atgaggtgga
     2041 cggacacgag ctccgggcgg agaaagctcg caagctccgc ggcgtcgctg gtcggtacgc
     2101 tctcatccgc cgtgccgtgg acgatgagcg cgggagccct cacgcggcga gcgtgctcgc
     2161 gcacgtccca tccggcgagg tcgtcgagaa acgaaacctt gagcatcggc ccaccgggcc
     2221 caagggcgat ggcctgccgt gcgcgccatt ccggctcgcg tgccgcgaag ttgcgccgga
     2281 agcgggccgg cgcatcgaat acggccgaca tgggggcgat ggcgcgaaca tcctcgggcc
     2341 tgcgggcggc aagcaacacg gacaccagcc caccgaagct gtgtccggcc accccgaggc
     2401 gagtcgaatc gacttcgggc cgcgcaagca gccaatccaa ggcatcctcc ccgtcatcga
     2461 cctcgcccga tagcgtcaga tccgcaaaca agccctcgga ttcg
//
LOCUS       DATMBB010000246         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_212896, whole genome shotgun sequence.
ACCESSION   DATMBB010000246 DATMBB010000000
VERSION     DATMBB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212896"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VNL92_05325"
     CDS             <1..498
                     /locus_tag="VNL92_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO94167.1"
                     /translation="KENLVGELNRGWYQVAVALDFERSGIQAYAGGRRTLERLVQFAR
                     EHPHFVARNPQVRYELADRWVELNVGYNIAYRITWMQAAGKIPNTEASVSKLYGSELS
                     QRIARTGVALMGMHGELVEGKQAPLDGSVARGYMTSVASTVAAGTSEVQRGIIAGRGL
                     GLPRG"
     gene            890..1969
                     /locus_tag="VNL92_05330"
     CDS             890..1969
                     /locus_tag="VNL92_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94168.1"
                     /translation="MPDVAYTLPEYTYNRLERLVDGIEAKPGKPVEIGAFAAMEANLK
                     VRSVLGTLPNGLSDDDFVGILRLAMLTECATETYGRAIGERARMFDARWLERFNTNVW
                     IPDELSHATPYEMLLRDLGFAPAQLRRDIRETQQKAFEHKGGDTPVHVTAFGMIQEYL
                     TDHWHGLIGGLLRPSSPDGSRLVRQVKRRETLHTMWYRDMTALQVEAQPRYIAYVAEA
                     MAQFEMPGNSLVPELQSQASRWLPLMNADFEQLLRDLTRIIYEVMGNARSVGKLLGDL
                     AAELGIKLGPIPARHLNAAISRLGGPGHGLLGEALLERAGLRHLYQREPPNEASHPVA
                     RLRALLRSWVARQMESRLDLARLAG"
     gene            complement(1943..2743)
                     /locus_tag="VNL92_05335"
     CDS             complement(1943..2743)
                     /locus_tag="VNL92_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO94169.1"
                     /translation="MDLNGARVWLLGASSGIGAALVPELTRAGAKLAISARREDALQR
                     VAERWGTTQRPIVVKPLDVTERYATDRAADELRQLWGAIDVLIYGAGDWEVTDVARFD
                     VDAIERQIMVNYVGLVRAVGAVLPAMLERRRGQIVGIASLSGYRGLPRAEAYGSTKAA
                     ANVFLQSLRIDTRHSGISVTTVNPGFVRTRLTAKNDFPMPFLLTPQQAARSIVLGIRR
                     DKAEVHFPLRLSVPLKLFTALPRPVYEWALTRGRRPSPAASPSQASEV"
     gene            2770..>3315
                     /locus_tag="VNL92_05340"
     CDS             2770..>3315
                     /locus_tag="VNL92_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWO94170.1"
                     /translation="MRVAVIGAGIAGLTAAHMLRPIHQVTLYEGESRAGGHANTIDVC
                     EGARSLALDTGFIVYNEHTYPGFTRLLAELGVATRPSDMSFSVSCRACRLEYSSAGLR
                     GLLAQPWSALRPQTHALLVDLLRFYGDAARALRTGEADGCSLSQWARDRRYSGSFWRH
                     FLAPLGAAVWSTRPADIGQFPA"
BASE COUNT          569 a         1111 c         1074 g          561 t
ORIGIN      
        1 aaggagaacc tcgttggcga gctgaaccga ggatggtacc aggtcgctgt tgcgctcgac
       61 ttcgagcggt ccggcatcca ggcatatgcg ggcggaaggc gcacgctcga gcgcctcgtt
      121 cagttcgccc gcgagcatcc ccacttcgtc gcacgcaacc cccaggttcg gtacgagctc
      181 gcggaccgct gggtcgagct caacgtcggg tacaacatcg cctatcgcat cacgtggatg
      241 caggccgccg gcaagatacc gaacacggag gcatcggtct cgaagctata cggctctgag
      301 ctgagccaac gcatcgccag aacaggtgtc gcgctcatgg ggatgcacgg cgagctcgtc
      361 gagggcaagc aagcgccgct cgatggaagc gtggcccgcg ggtacatgac atcggtcgca
      421 tcgaccgtag cggcaggtac gagcgaggtg cagcgcggca tcattgccgg ccgggggttg
      481 ggtctgccgc gcgggtgagc gctaccgcgg cacacgcgcc gagaccggga cgcgatcgtc
      541 ccggtctctc tgcgtcgcgc tcccgggcgg ccgcaacgcc ccggagcaac tggggaggcg
      601 cggctatcgt gcttgtgccc gaccaccgca gtccgcacat taccgcccca aggtttcggc
      661 cggattacct tcgcatcaca gtatggttac gccgcgcagg tcgtgtcatg gctgagaagg
      721 aactgggctt ccgcgaggtg cgatctcctc ttagtggtcc aattcttaca cactcgttac
      781 gctagaccca tacgcatcag actcgatgct tcgtgcgctc cgatgtacaa ttcattggac
      841 tgagcgccat ggccccagca atcgcctggc ctcgttgcgg gagagggact tgccggacgt
      901 cgcgtacacg cttccagaat atacgtacaa tcgcctcgag cgcctcgtgg atggcatcga
      961 ggccaagccc ggcaagccgg tcgagatcgg cgcattcgcg gccatggagg ccaacctcaa
     1021 agtccgctca gtactgggca cgctcccgaa cggcctcagc gatgacgatt tcgtcggcat
     1081 cctccgtcta gccatgctga ccgaatgcgc gacggagacg tacggccgcg ccatcggcga
     1141 acgggctcga atgttcgacg cacgctggct ggagcggttc aacacgaacg tctggatccc
     1201 cgacgagctg tcgcacgcca cgccgtacga gatgctgctg cgcgacctgg gcttcgcccc
     1261 cgcgcagctt cggcgcgata tccgagagac gcagcagaag gcattcgagc acaagggtgg
     1321 cgacactccg gtgcatgtga cggcgttcgg gatgatccag gagtacctga ccgatcactg
     1381 gcatggcctc atcggagggc tgctccgccc gtcttcgccc gatggttcgc gactcgtccg
     1441 gcaggtcaag cgccgcgaga cgctccacac gatgtggtac agggatatga ccgccctcca
     1501 ggtggaggcg cagccccgct acatcgcgta cgtcgccgag gcgatggcgc aattcgagat
     1561 gcccgggaac tcactcgtgc cggaactgca gtcgcaggcg tcacgatggc tgcctttgat
     1621 gaacgcggac ttcgagcaac tcctgcgcga cctcacccgc atcatctacg aggtaatggg
     1681 caacgcccgg agtgtgggca aactgctcgg cgacctcgcg gccgagcttg gcatcaagtt
     1741 gggtccaata cccgctcgac acctcaacgc ggcgatctcc cgcttgggcg ggcccggcca
     1801 cggcctcctc ggtgaggcgc tgctggaacg cgccggcctg cgtcacctgt accaacgaga
     1861 accgccgaac gaggcgtccc acccggtcgc gcggctccgt gcgctgctgc gctcctgggt
     1921 cgcgcgccag atggagagcc gcctagacct cgctcgcttg gctgggtgag gcagccggcg
     1981 atggacggcg gccgcgggtc agcgcccatt cgtacaccgg ccgaggcagg gcggtgaaca
     2041 gcttgagcgg caccgaaagg cgcagcggga aatgaacctc cgccttgtcg cgccgaatgc
     2101 ccagcacgat gctccgcgcc gcttgctgcg gtgtcagcag aaacggcatc gggaagtcgt
     2161 tcttggcggt cagacgagtc cgcacgaatc ccgggttcac ggtcgtcacg ctgatgccgg
     2221 agtgcctcgt atcgatcctc agcgattgga gaaacacgtt ggccgctgct ttcgtcgagc
     2281 cgtaagcctc ggcgcgtggc aaaccgcgat accccgagag gctcgcgata ccgacgatct
     2341 gccctcggcg gcgttccagc atcgcaggca ggacggcgcc gaccgcgcgt acgaggccga
     2401 catagttcac catgatttgc cgttcgatgg cgtcgacatc gaatcgtgcg acgtcggtga
     2461 cctcccaatc gccagcgcca tagatcagga cgtcaatcgc cccccacagt tgacgcaact
     2521 cgtctgcggc gcgatcagtc gcgtagcgct ccgtcacatc gagcggcttg acgacgatcg
     2581 ggcgctgcgt cgtcccccag cgctccgcga cacgctggag cgcatcctcc cggcgagcgc
     2641 ttatcgccag cttggccccg gcccgcgtca gctccggcac cagcgcagcc ccgattccgg
     2701 agctcgcccc gaggagccaa acacgagcgc cattcaggtc catttctcgg caccttcgtt
     2761 cgccggatca tgagggtcgc cgtcatcggt gcgggcatcg caggactcac ggccgcccac
     2821 atgttgcgtc cgatccacca agtcacgctc tatgagggcg agtcgcgggc gggcggtcat
     2881 gcgaacacga tcgacgtgtg cgagggcgcg cggtcgcttg cgctcgatac ggggttcatc
     2941 gtctataacg agcatacgta ccccggcttc actcgccttc tcgccgagct gggcgttgcg
     3001 acgcgaccca gcgacatgtc gttcagcgtc tcctgtcgcg cctgccgact agaatatagt
     3061 agcgccggcc tgcgcgggct tctcgctcag ccgtggagtg ccctccgtcc gcagacgcac
     3121 gcgctcctgg tggacctgct gcggttctac ggcgatgccg ctcgcgcgct tcggacggga
     3181 gaggcggacg gctgctcgct gagtcaatgg gctcgcgacc ggaggtacag cggctcgttt
     3241 tggcgccact tccttgcgcc gctgggagcg gcggtctggt cgacgaggcc ggcggacatc
     3301 ggacagtttc cggcg
//
LOCUS       DATMBB010000247         2072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_226059, whole genome shotgun sequence.
ACCESSION   DATMBB010000247 DATMBB010000000
VERSION     DATMBB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226059"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VNL92_05345"
     CDS             complement(<1..474)
                     /locus_tag="VNL92_05345"
                     /inference="COORDINATES: protein motif:HMM:NF040248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94171.1"
                     /translation="MFAYTLRRVLAIIPLILAVATITFLLMHAVKGGPFDSDKPLSPA
                     ARANLERRYGLSDPLPQQYIKYITNLARGDLGISFANDRPVRTIIRERMEVSVQLGIA
                     TFLFATVFGLGLGSLAALNQNGIGDYVGVFLATAGAALPSFVLSPILQIVFAVELG"
     gene            complement(542..>2072)
                     /locus_tag="VNL92_05350"
     CDS             complement(542..>2072)
                     /locus_tag="VNL92_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWO94172.1"
                     /translation="TEFENWDPHFSDFAQDIAHFFKVWRGMYELDLDNKPIPSMADGE
                     PQVSEDGKTYTIKIKSGLKWSDGDDLKAEDFVAGIQRTCNPDIAGHYQYILTAIVGCD
                     DYYAAAEASDAEKEELLQALGVRAVDDLTVEIKLTDPQPTFPILLALWPTFPVPVHLV
                     SEVDGAWPEPTANVYNGPFMVSAYTEGSNMELVPNPNWSGEQKPYLDKIILQYVDDAA
                     VLNNAYRANEVQATVANKPELDALQSEFPDEVHLYPATRTIGIEFMLEDPTLAKPGVR
                     LALSQATDRETMMDVVFSGANTPTTSWVPPVRNGLEGGEYDDILGFNPESAKKHLADA
                     GYANGQGFPKLVLLLTDSQTNKLAGEFLQAEWKKHLNIDIELEFVDGPTRAARFNASD
                     FQLVLGGWQEDYPDPENWFLGLWETGGSINKVKVSIPELDDVIDRARFNTNDEERREL
                     YREAERILLREANGIAPIYHTLAAFLVKPNIRGMVEWKRPGDTFVPGDWNPEYWALAA
                     N"
BASE COUNT          361 a          630 c          688 g          393 t
ORIGIN      
        1 gcccaactcg accgcgaaga cgatctggag gatcggcgag aggacaaagc tcggcagcgc
       61 tgcgcccgcc gtcgcaagga agacgccaac atagtccccg atgccgttct ggttcagcgc
      121 ggccaggctg ccgagcccaa ggccgaagac cgtcgcgaaa aggaatgtcg cgatgccgag
      181 ctggaccgac acctccatcc gctcacggat gatcgtccgg acaggcctgt cgttggcgaa
      241 cgagataccc aggtcgcccc gggcgaggtt cgtgatgtat ttgatgtatt gctgcggaag
      301 ggggtcgctg agcccatatc gccgctcgag gttcgcacgc gcagcgggcg aaagcggctt
      361 atcgctatcg aaggggccgc ccttcactgc gtgcatcaac aaaaacgtga tcgtcgcaac
      421 cgcgaggatc agggggatga tggcgaggac gcggcgcagc gtataggcga acatcggcgg
      481 tccggttcag gtggaggggg cccggaccgg ccgggccccc tttgtgcgtg cgtagccgga
      541 tttagttggc tgccagcgcc cagtactcgg ggttccaatc gcccggcacg aacgtatcgc
      601 cggggcgctt ccactccacc atgccgcgga tgttgggctt cacgaggaag gcggcaagcg
      661 tgtgatagat gggcgcgatg ccgttggctt cgcggagcag gatccgctcg gcttcgcggt
      721 aaagctcgcg ccgttcttca tcgttcgtgt tgaagcgcgc acggtcgatg acgtcgtcga
      781 gctcgggaat gctcaccttc accttgttaa tcgacccgcc cgtctcccag agcccgagga
      841 accagttctc cggatcgggg tagtcctcct gccagccacc aagcacgagc tggaagtcgc
      901 tcgcgttgaa gcgcgcggcc cgggtcggac cgtcgacgaa ctcgagctcg atatcgatgt
      961 tcaggtgctt cttccattcc gcctgcagga attcgccggc gagcttattc gtctgcgaat
     1021 cggtcagcag gagcacaagc ttggggaagc cctggccgtt ggcatagccg gcatcggcaa
     1081 ggtgcttctt cgccgactcg gggttaaacc cgaggatatc gtcgtactct ccgccctcaa
     1141 gaccgttccg gacgggcggc acccagctcg tcgtcggcgt attcgcgccg ctgaagacga
     1201 cgtccatcat cgtctcgcgg tccgtcgcct gcgagagcgc aaggcgaacg cccggcttgg
     1261 ccagcgtcgg gtcctcgagc atgaactcga tgccgatcgt gcgcgtcgca ggatagagat
     1321 gaacctcgtc cgggaactcc gactgcagcg cgtcgagttc cggcttgttg gcaaccgtgg
     1381 cctgcacttc gttcgcgcga tacgcgttgt tgaggacggc tgcgtcgtcg acgtactgga
     1441 ggatgatctt gtcgagatac ggcttctgct cgcccgacca gttcgggttc gggaccagct
     1501 ccatgttcga gccttcggta tacgccgaga ccatgaaggg gccgttatag acgttcgccg
     1561 tgggctccgg ccacgcgccg tcgacctcgg agacgaggtg gaccggcacc gggaaggtcg
     1621 gccacagcgc cagcagaatg gggaacgtgg gctgcgggtc cgtcagcttg atctcgaccg
     1681 tgaggtcgtc caccgcgcgc acaccgagcg cctggagcag ctcttccttc tcggcatcgc
     1741 tggcctcagc ggcagcgtag tagtcgtcgc agccgacgat cgcggtaagg atgtactggt
     1801 agtggccggc gatgtcggga ttgcaggtcc gctggatgcc ggcgacgaag tcctcggcct
     1861 tgaggtcgtc accatcggac cacttcaggc cactcttgat cttgatggtg taggtcttgc
     1921 cgtcctcgga gacctggggc tcgccatcgg ccatcgatgg gatgggcttg ttatcgaggt
     1981 cgagctcata catgccccgc cacactttga agaagtgggc gatgtcctgg gcgaagtccg
     2041 agaaatgcgg gtcccagttt tcgaattcgg tg
//
LOCUS       DATMBB010000248         3436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_176161, whole genome shotgun sequence.
ACCESSION   DATMBB010000248 DATMBB010000000
VERSION     DATMBB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176161"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VNL92_05355"
     CDS             complement(<1..414)
                     /locus_tag="VNL92_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94173.1"
                     /translation="MHSGSAALLLSDGQVRQTAGAPGDAFFRLDVHYITEAGTPSIGL
                     AWLDEAGVQITMRTLALASAGPGYTPFTIAGVSPSRARAVEVMFLAPPGAILHVDDVS
                     LDMGESVPIDDPAPPPTSQPDEAESAAPGPEPPSER"
     gene            714..1460
                     /locus_tag="VNL92_05360"
     CDS             714..1460
                     /locus_tag="VNL92_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWO94174.1"
                     /translation="MDEGKPDYTLLEFPAPPDARPYVVINMVASLDGRVVIEGTERGL
                     GSKVDQRLMRELRYHADVVMNGANTLRASGTSSRVGDAELEERRIAQGKTRHPIAAVI
                     TASGSLPLDRSFFTARDFDAIVYLSERTPEDRRRAIEATGRRVQVLRAGEEIHAMLRH
                     MRAELGASLLLVEGGPTLNAELFDLDAVDELFLTLGGLIVGGRDPLTMIREGRERSAE
                     HVRRMDLVHAVPNRATNEVYLRYRRPSREA"
     gene            1524..2789
                     /locus_tag="VNL92_05365"
     CDS             1524..2789
                     /locus_tag="VNL92_05365"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94175.1"
                     /translation="MAWAGPLAGVRLADFCWWGVGALCTRMLADFGAEVIKIEDRHKM
                     DMIRTAPPLPGRVAALGQVGGEVDPNTSGIFNNHNRNKLSIVINMRDQRGRELCRRLI
                     AKSDVVSENFRPGVMERWGLDYEALRAIRGDIIFARLSGLGHSGPESPYGTAGPVVQA
                     LCGLTAMAGLPGREPSGWGYSYMDNTAAYYGTIAIMLALYHKRATGLGQEIDVSAVEA
                     GINLLGPDLLDFMANQRPYKRDGRPEGNRLSSPPAAPHGVYPTNEEDRWIAIAVFSDA
                     EWEGLLDVMARPAWANDPRFATLGKRLKHQDELDAHLAEWTSGQERYDLMTRLQATGV
                     PASVVQTPEDRCERDPQIRLRELFPIVDHPVLGPYPTEGLSFKMSASPPKEGWRGAPC
                     LGEHTALVLTEVLGMSSREVDELAEAGVI"
     gene            2789..>3436
                     /locus_tag="VNL92_05370"
     CDS             2789..>3436
                     /locus_tag="VNL92_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94176.1"
                     /translation="MTSETSGRKPRPLQGLQVLELADEMGVYAGKLLADAGARVIKVE
                     PPGGSAMRHRGPYLNDDPGPDRSLYFAFYNTGKESVTLDLGDEDDREVARRLASQADV
                     VIDGMGLGRLASAGLSYEALRGDNPGLIWLQITPFGPDGPWASYKTSDLVALALGGPL
                     FDCGYDDHSIPPIRGGGNQGYAIVSNLAMCAVMVALLWRQQSGEGQHIDLSMHDAC"
BASE COUNT          578 a         1098 c         1191 g          569 t
ORIGIN      
        1 ccgctccgaa ggtggctcag ggcctggcgc cgccgattct gcttcatcgg gctggctcgt
       61 cggcggggga gcgggatcgt cgatggggac ggactcgccc atgtccaacg atacgtcgtc
      121 gacatggagg attgcccccg gaggagccag gaacatcacc tcgaccgcgc gggcgcgcga
      181 aggcgatact ccggctatcg taaagggtgt gtacccgggc ccggctgagg cgagcgccag
      241 cgttcgcatc gtgatctgta cgccggcctc gtcgagccat gcgaggccga tgctcggcgt
      301 gccggcctcg gtgatgtagt ggacgtcgag gcgaaagaag gcatcgccgg gggcgccggc
      361 cgtctggcgg acctgcccgt cgctcagaag tagtgcggcg ctgccggagt gcactatcgt
      421 ggcatcgagc gatgccccat ccgtgtttgc ccacccggcg aggccttcct caaagccgtc
      481 gttctttaat atgggtgggg agggtgtggg ggagggccct gcgctgccga tgctgcaaat
      541 gagggccagc gcaagtgcgg ttggcgccca gaatcgggcg tggcacagct gtcgaaacag
      601 ccgcaaagtt tggtccccgc gtgtcccccg cccgggcccg agttgcagcg cgtagcatag
      661 gtggtggctc cggcgtgtca agcccctggg cttcagggag aggagagggt cgattggacg
      721 agggtaagcc agactatacg ctccttgagt ttccggcgcc gccggatgcg cggccctatg
      781 tcgtgatcaa catggtggca tctctcgatg ggcgcgtcgt catcgagggt acggaacgcg
      841 gattgggctc gaaggtcgac cagcggctga tgcgcgagct gcgctaccat gcggacgtcg
      901 tcatgaacgg cgccaacacg ctgcgcgcga gcggcacatc ctcgcgcgtg ggcgatgcgg
      961 agctcgagga gcggcgaatc gcccagggca agacacggca tcccatcgcc gcggtcatca
     1021 ccgcttcggg ctccctcccg ctcgaccgct cgttcttcac tgcccgagac ttcgatgcca
     1081 tcgtgtacct ctcggagcgg acgcccgagg atcggcggcg tgcgatcgag gccaccggac
     1141 ggcgcgtgca agtgctccgc gcgggcgagg agatccacgc gatgttgcgc cacatgcgcg
     1201 cggagctcgg ggcctcgctc ctgcttgtcg agggagggcc cacactcaac gcggaactct
     1261 tcgacctcga cgcggtcgac gagctcttcc ttacgctcgg cgggctcatc gtcggggggc
     1321 gtgatccgct cacgatgatt cgagaagggc gggagcgctc cgccgagcac gtacgccgca
     1381 tggacctcgt gcatgcggtc ccgaaccgcg cgacgaacga ggtgtatctc cggtaccggc
     1441 gtccttccag ggaggcatga cggatggaca ggttcgacca ctgctgctag gattcgcgca
     1501 cctgcagatg agggggtgcg cctatggcgt gggcaggtcc gctggccggc gtccgtctgg
     1561 ccgatttctg ctggtggggc gtaggagcgc tctgcacacg aatgctcgcc gacttcggtg
     1621 ccgaggtcat caagatcgag gatcggcaca agatggacat gatccgaacg gccccgcccc
     1681 tgccgggacg ggtcgccgcc ctcggtcagg ttggcggcga ggtcgacccc aacacgagcg
     1741 gcatcttcaa caatcacaac cgcaacaagc tcagcatcgt gatcaacatg cgcgaccagc
     1801 gcggccggga gctctgccgc cgtctcatcg cgaaaagcga cgtcgtgagt gagaacttcc
     1861 gccccggggt gatggaacgc tgggggctcg attacgaggc gctgcgcgcc atacgaggcg
     1921 atatcatctt cgcccgcctg agcggcctcg ggcattccgg ccccgaatcg ccctacggca
     1981 ctgccggtcc cgttgtgcag gctctctgcg gtttgacggc gatggcaggc cttccggggc
     2041 gggagccgtc cggctggggc tattcctaca tggacaacac ggccgcctac tacggcacga
     2101 tcgccatcat gttggcgctg tatcacaagc gcgcgacggg gctgggtcag gagatcgacg
     2161 tttcggccgt cgaggccggg atcaacctgc tcggcccgga cctgctcgat ttcatggcca
     2221 accaacgccc gtacaagcga gatggccggc cggagggcaa tcggctctcg agcccgcctg
     2281 ccgctccgca tggtgtgtac cccacgaacg aagaggatcg ctggatcgcc atcgccgtct
     2341 tctcggacgc tgaatgggaa gggctgctcg atgtcatggc tcgtcccgcg tgggcgaacg
     2401 atcctcgctt cgcgacgctc ggcaagcgcc taaaacacca ggacgagctc gatgcacacc
     2461 tcgccgagtg gaccagcggc caggaacggt acgacctgat gacacgcctg caggccacag
     2521 gggttccggc cagcgttgtc cagacgccag aggaccgctg cgaacgtgac ccgcagattc
     2581 gcttgcgaga gctcttcccc atcgtcgatc atccggtcct ggggccgtac ccgaccgagg
     2641 ggctttcctt caagatgagc gcaagcccgc caaaggaggg atggcgcggg gcgccctgtc
     2701 tgggcgagca caccgcactc gtactcaccg aggttctcgg catgagctct cgggaggtcg
     2761 acgagctcgc cgaggcgggg gtgatctgat gacgtcagag acaagtgggc ggaagcctcg
     2821 ccccctgcaa ggactgcagg tcctggagct ggcggacgaa atgggcgtct acgccggcaa
     2881 gctcctcgcc gatgccggcg cccgcgtcat caaggtcgaa ccgcctggcg gttcggcgat
     2941 gcgtcaccgc gggccgtacc tgaacgatga tcccgggccc gaccgcagcc tgtacttcgc
     3001 gttctacaac acgggcaagg aatcggtgac gctggacctg ggcgacgagg acgaccgcga
     3061 ggtcgcgagg aggctggcat ctcaggccga cgtcgtcatc gacggtatgg gactgggccg
     3121 cttggcatcg gcgggcctga gctacgaggc gctgaggggt gacaatccgg gactgatctg
     3181 gctgcagatc acgcccttcg gtcccgacgg gccgtgggcg agctacaaga cctcggacct
     3241 cgtcgcgctg gcgctgggcg ggcccctgtt cgattgcggc tacgacgatc acagcatccc
     3301 gcccatacgg ggcggtggta accaaggcta cgctatcgtc tcgaacctcg cgatgtgcgc
     3361 ggtcatggta gcgttgctct ggcgtcagca atccggggag gggcagcaca tcgacctctc
     3421 gatgcacgac gcttgc
//
LOCUS       DATMBB010000249         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_588441, whole genome shotgun sequence.
ACCESSION   DATMBB010000249 DATMBB010000000
VERSION     DATMBB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588441"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1244)
                     /gene="dnaN"
                     /locus_tag="VNL92_05375"
     CDS             complement(141..1244)
                     /gene="dnaN"
                     /locus_tag="VNL92_05375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWO94177.1"
                     /translation="MLKLDCPRKEFFEAVSAASAAASVRTSINILQNLKIDAHDAGLR
                     VVGCDGEMWVERNVACMVGEPGSVCLQARLLSDLVGKLAEGDVQLKTLNGHGAMLQQG
                     ASEYRLQTLDASDFPEPPDLSPEAELTLPMGTLRHAIDSVIFAVSTDTHRQVLTGVLF
                     RYDGAVLTLVATDTHRLAVRRIEQPGIGSEVTAVVPEKALKAIKALPVTDEESITLRF
                     GAGRLSVEAGGARVVSQLLSGAYPNWERVVPTEATRTWSVECDQLEEKVGRIMILARD
                     NANRVRFRGQGDQIVIAARSEERGEAKEEVPMVATNGDIEIAFNGKYVEDALGPISGP
                     GVKIEMTESSRPAVFKGTEDDGYFCVIMPMALA"
     gene            complement(1258..1542)
                     /locus_tag="VNL92_05380"
     CDS             complement(1258..1542)
                     /locus_tag="VNL92_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94178.1"
                     /translation="MNDLYRKLVDLYAGRELPAELEAELEERAKDEPELAYDMRSLRA
                     TVDNLRASDDVEMTEESYQRILMRLYTRGVDVQPQAPAPAHLQYYLPIQG"
     gene            complement(1539..>2127)
                     /locus_tag="VNL92_05385"
     CDS             complement(1539..>2127)
                     /locus_tag="VNL92_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWO94179.1"
                     /translation="TPRASLPGASPSYPMPNDALRDSLAEKRWLTALANGDSSALGGL
                     YEMYGERIFRYTYRMLGNRTDAEDATAETFLRVLRRSTELRADGAFRTWLFRIARNLC
                     IDKLRQHKLMELPPDSHDAGTEERTTLRLTVRQALEDLPIEYREPLILCDLEDMAARE
                     AAELLKISVPALKSRLYRGRRALRDKLGGTWERTS"
BASE COUNT          300 a          730 c          710 g          387 t
ORIGIN      
        1 tgctcttcca cgtgatcaac tttcggctgc aggcgcggtg cggctgcgac cgctgccgca
       61 cggggcattg agccgacggc ggggaggccc cccccgggga aacgtcccat ccggcgagcg
      121 gcccggggcc tagggccccg ctacgccagc gccatgggca tgatcacgca gaagtagccg
      181 tcgtcctccg tgcctttgaa caccgcgggc cggctgctct ccgtcatctc gatcttcacc
      241 ccggggccgg agatggggcc gagggcgtct tccacatact tgccgttgaa ggcgatctcg
      301 atgtccccgt tcgtcgcgac catcgggacc tcctctttcg cctcgcctcg ctcctcgctg
      361 cgcgccgcga tcacgatctg gtccccttgg ccgcggaacc ggacccggtt ggcgttgtcc
      421 cgggcgagga tcatgatgcg cccgaccttc tcctcgagct ggtcgcactc cacgctccag
      481 gttcgcgtcg cctcggtggg caccactcgc tcccagttgg ggtaggcgcc gctgaggagc
      541 tgcgagacga cgcgcgcgcc gcccgcttcc acggacagcc ggccggcgcc gaatcgcaag
      601 gtgatcgact cctcgtcggt caccggcagc gccttgatgg ccttcagcgc cttctccggc
      661 accaccgccg tcacctcgct gccgatgccg ggctgctcga tgcgccggac cgcgagccgg
      721 tgggtgtcgg tggccacgag cgtcagcacc gcgccgtcgt agcggaacag cacgccggtc
      781 agcacctggc ggtgggtgtc ggtggagacg gcgaagatca ccgagtcgat cgcgtgcctc
      841 agcgttccca tgggcagggt cagctcggct tcaggcgaga ggtcgggcgg ctccgggaag
      901 tcggaggcgt ccagcgtctg cagccggtac tcggaggcgc cctgctgcag catggcgccg
      961 tggccgttca gggtcttgag ctggacgtcc ccttccgcga gcttgccgac gaggtcgctg
     1021 agcaagcggg cctgcaggca gacggagccg ggctcgccca ccatgcaggc gacgttgcgc
     1081 tcgacccaca tctcgccgtc gcatccgacc acgcggagcc ccgcgtcgtg ggcgtcgatc
     1141 ttcaggttct gcaagatgtt gatcgaggtt cgcacgctcg cggccgccga ggcggcgctg
     1201 acggcttcga agaactcctt gcgcggacaa tccagcttca acacgtagtc tcctcgtcta
     1261 gccctggatg ggcaggtagt actgcaggtg cgccggggcg ggggcttgcg gctgcacgtc
     1321 caccccgcgc gtgtagaggc gcatgaggat ccgttggtag gattcctcgg tcatttcgac
     1381 atcgtcggac gcgcgcaggt tgtcgaccgt ggcgcgcagc gagcgcatgt cgtaggcgag
     1441 ttcgggctcg tccttggccc gctcctcgag ctcggcttcc aactccgccg gcagctcgcg
     1501 cccggcgtat aggtccacca gtttccggta aaggtcgttc atgacgttct ctcccaagtt
     1561 cctcctagtt tgtcgcggag ggccctacgt cctctgtaaa gcctcgattt cagcgcggga
     1621 acgctgatct tcaggagctc cgcagcctcc cgggccgcca tgtcctccag gtcgcagagg
     1681 atgagcggct ccctatattc gatcggcagg tcctcgagcg cttgccgcac cgtgaggcgc
     1741 agcgtggtcc gctcttcggt ccccgcgtcg tgcgagtcgg gtggcagttc catgagcttg
     1801 tgctgccgga gcttgtcgat gcacaggttc cgggcgatcc ggaacagcca cgttctgaag
     1861 gcgccgtcgg cacgcagctc cgtcgatcga cggagcaccc gcaagaacgt ctctgcggtc
     1921 gcgtcttccg catccgtgcg gttgcccagc atgcgatagg tgtagcggaa gatccgctca
     1981 ccgtacatct cgtacaggcc gcccagcgcg gaggaatccc cgttcgccag ggcggtgagc
     2041 cacctctttt cggccagcga gtctcgcagt gcgtcattcg gcatagggta ggacgggctc
     2101 gcacccggca aggatgcgcg aggcgta
//
LOCUS       DATMBB010000250         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_320542, whole genome shotgun sequence.
ACCESSION   DATMBB010000250 DATMBB010000000
VERSION     DATMBB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320542"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VNL92_05390"
     CDS             <1..446
                     /locus_tag="VNL92_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94180.1"
                     /translation="IPPLSRETQEALLEFTPFAGTSVRNPIDTAVFGDPELVRRTLEL
                     VAKDPAIDLILYPTGFGGMPGSATLDPEEQAERALAPARAVEREIGKPVVYVIRPPMT
                     QDGLEATQVFQERAAEEGFAVFPSVARAARAIAALFERQRLLMEE"
     gene            complement(469..933)
                     /locus_tag="VNL92_05395"
     CDS             complement(469..933)
                     /locus_tag="VNL92_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWO94181.1"
                     /translation="MSFTIIEEKCISCGACEFVCPTVAVHKPVVGAASPSFWIETNRC
                     NDCDACAPVCPTYAIVVDPDAYVCYGKGCPVATHTTGPITGWLCTELTRFCEHCGDVL
                     HRESEGSDWVCVQCDLGIKARCPKARKLEAEKHSRPMLQYSREDFESLTDLL"
     gene            1239..2237
                     /locus_tag="VNL92_05400"
     CDS             1239..2237
                     /locus_tag="VNL92_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94182.1"
                     /translation="MVAQVTEVVPGIHQFKVPLPMEYLPYVLVYCIRGDDGYTLIDCG
                     WNTEEAWNALTDQLRSVGADVRDVAHLIVTHIHPDHYGLAGRLKEATGCEVVLHEREQ
                     KMVPGRYTDPSGLLDHMKVWLKRQGVPEALANELHNASMPTRQFVTEVEPDTKIRGGE
                     RRRIGPYTFEALWTPGHSPGHICLVEHEAGFMMTGDHVLPSITPNVSLHPQQEGNPLG
                     EYIESLEKIKPLNIRRGLAAHEHVIEDLPKRLEELEEHHHRRLEEMLRAVASGAETAH
                     DVAAAIVWTTGTFDTFSPWQKRAAISETLAHLEYARSRGMVTAVERGGVVRYLRVD"
     gene            2336..2524
                     /locus_tag="VNL92_05405"
     CDS             2336..2524
                     /locus_tag="VNL92_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94183.1"
                     /translation="MSESAEVPERSDSDRRDRRVVVGDQQCPRCHEWFTLSYWNADFL
                     IQARCFSCDLRWPRPDVV"
     gene            2555..3055
                     /locus_tag="VNL92_05410"
     CDS             2555..3055
                     /locus_tag="VNL92_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWO94184.1"
                     /translation="MNFEDLKNQTVISIDEGERMGKVTDGVIDLDKKQLVALLLGSGG
                     IIGSKNYTLPVDAIRTVGRDAVTVQSRSMLREGEDVLDGQVRVSDLARRTVISEGGDE
                     IGRISDVELDTSYHVTRFHISTGSRLLSMFGSTAMIDAGQVVAFGEVITVKDGAVPSK
                     EESGKG"
     gene            3060..>3375
                     /locus_tag="VNL92_05415"
     CDS             3060..>3375
                     /locus_tag="VNL92_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWO94185.1"
                     /translation="MELPRNLDVRSLGVGDQPLPRFASVRQSFRDDSLPDAALAVREA
                     LDRSGLQDRLPTGGRIAITAGSRGIDRIAEATRAVVDWVRAAGCEPFVVPAMGSHGGA
                     TAE"
BASE COUNT          618 a         1081 c         1107 g          569 t
ORIGIN      
        1 agatcccgcc gctatcgcgc gagacgcagg aggcccttct cgagttcacc ccgttcgctg
       61 ggacgagtgt gcgcaacccg atcgacaccg cggtctttgg cgacccggag ctcgtgcgcc
      121 gcaccctcga gctcgtcgca aaggaccccg cgatcgatct catcctctac cccaccggct
      181 tcggcggaat gccgggcagc gccacgctgg atccagagga gcaggcggag agggcactgg
      241 cccccgcccg tgctgtggag agagagattg gaaagcctgt cgtgtatgtc attcgtccgc
      301 caatgacgca ggatgggctc gaggcgacgc aggtcttcca ggagcgcgcg gcggaggaag
      361 ggttcgccgt gttcccatcc gtcgctcggg cggcacgtgc catcgcggcc ctgttcgagc
      421 gtcagaggct gttgatggag gagtagcggg agtcgctctt gcccgctgtt agagcagatc
      481 cgtcaagctc tcgaaatcct cgcggctgta ctgcagcatc ggccgactgt gcttctccgc
      541 ctcgagcttc cgagcctttg ggcagcgagc cttgatgccg agatcacact gcacgcagac
      601 ccagtcgctc ccctcgctct cccggtggag cacgtcgcca cagtgctcgc agaagcgcgt
      661 gagctcggtg caaagccagc cggttatcgg gccggtggtg tgtgtcgcta cgggacatcc
      721 cttcccgtag cagacatagg cgtcggggtc gacgacgatg gcatacgtcg ggcacaccgg
      781 ggcgcaggcg tcgcaatcgt tacagcggtt tgtctcgatc cagaaggagg gactggctgc
      841 tccgacgacc ggcttgtgga ccgcgaccgt cggacagacg aactcgcacg ccccgcagga
      901 gatgcacttc tcctcgatga tggtgaagct caagcggtat gctcctccca acgacgcgac
      961 ctcggggagg aggcgcttcg ttaattagtg gaacgattgt acacgacgga tgcgtgatag
     1021 cctcgttcga attcttgccc gccgtgcaac cggagcggct cggcacgcgg aaccgcgagg
     1081 aagccggcga aacgtttacg ttcgcgcaaa ggattgaccc gcttcgcgcc tctgtgtcag
     1141 actgtcgtcg tgggccgtgc gaggcccacg acgtgtgttt ggcattgaca ggagggccgc
     1201 gcatcccggt tgggagcgcc agcccggaag gggccaacat ggtagctcag gtcacagaag
     1261 tcgtccccgg gattcaccag ttcaaagtac cgctccctat ggagtatctc ccctatgtcc
     1321 tcgtgtactg cattcgcggc gacgacggct atacgctcat cgactgtggg tggaacaccg
     1381 aagaggcgtg gaacgcgctc acggatcagc tccgaagcgt cggagcggac gtgcgcgatg
     1441 tcgcccacct catcgtcacc cacatccacc cggaccacta cgggctggcg gggaggttga
     1501 aggaagcgac cggatgcgag gtggtgctcc acgagcgcga gcagaagatg gtgcccggcc
     1561 gctacacgga tccgtccggg ctgctcgatc acatgaaggt gtggctcaag cggcagggcg
     1621 tacctgaagc tctcgcgaac gaactgcaca atgccagcat gccaacgcgg caattcgtga
     1681 ccgaggtgga acctgacacg aagattcgcg gtggcgagcg gcgccgcatc ggcccctaca
     1741 cgttcgaggc gctttggacc ccggggcact cccccggcca catctgtctc gtcgaacacg
     1801 aggccggctt catgatgacc ggcgaccatg tgctgccgtc aatcacccca aacgtgagcc
     1861 tgcacccgca gcaagaaggc aatccgctcg gcgagtacat cgagtcgctc gagaagatca
     1921 agccactgaa cattcgccgt ggattggccg cgcacgagca cgtcatcgag gacctgccga
     1981 agcgactcga ggagttggag gagcatcacc accgccggct ggaggagatg ctccgggccg
     2041 tggcgagcgg cgccgagacc gctcacgacg tcgctgcggc aatcgtctgg acaacgggca
     2101 cgttcgacac gttctccccc tggcagaagc gcgcggccat cagcgaaacc ctcgcccacc
     2161 tcgaatatgc tcggtcacgc ggcatggtca ccgctgtcga gcgcggtggc gtggttcgtt
     2221 accttcgcgt cgactaagcg gcgtccaacc taccccaacg cgtggtgcca cgcgaatcgc
     2281 cccggctgct gttttccgcc gccgcctgct agaatgcggt cgaaagggga tggtggtgag
     2341 cgaatcggca gaggtgccgg agcgcagcga ctcggaccgc agggaccggc gcgtcgtcgt
     2401 cggcgaccag cagtgcccgc gctgccacga atggttcacg cttagctatt ggaacgccga
     2461 cttcctcata caggcgcgct gcttcagttg cgatttgcgg tggccgcgcc ccgatgtcgt
     2521 ctaggaccga ggacaaccaa ggggagcacg accagtgaac ttcgaagacc tcaagaatca
     2581 gaccgtgatc tccatcgacg agggcgagcg gatggggaag gtcaccgatg gcgtgatcga
     2641 cctggacaag aagcaactcg tggcgctcct cctcggatcg ggcggcatca tcggaagcaa
     2701 gaattataca ttgccggtcg acgccatccg caccgtgggg cgcgatgcgg tcacggtcca
     2761 gagtcgctcg atgcttaggg aaggcgaaga cgtgctggat gggcaggtgc gcgttagcga
     2821 tctcgcgcgc cggacggtga tcagcgaggg cggcgacgag attggccgca tttccgatgt
     2881 cgagttggac acgtcctacc acgtcacccg gttccacatc tccaccggct cgcgcctcct
     2941 ttcgatgttc gggagcacgg ccatgatcga tgcgggccaa gtcgtcgcgt tcggcgaagt
     3001 cataacggtc aaggacggcg ccgtgccctc caaggaggag tcgggcaaag ggtagggcga
     3061 tggagctgcc ccggaacctc gacgtccgct cgctcggcgt cggcgaccag cccttgcctc
     3121 gtttcgcctc cgtccgccag agcttccgcg atgactccct gcccgatgct gcactcgccg
     3181 tccgagaggc gctcgaccgc tcgggcctcc aggatcgcct cccaacggga ggtcgcatcg
     3241 ccatcacggc cggtagccgc ggcatcgacc gcatcgcgga ggccacacgc gcggtagtcg
     3301 attgggtgcg cgcggcgggc tgcgaacctt tcgtcgtacc tgccatggga tcgcacggcg
     3361 gtgcgacagc cgaag
//
LOCUS       DATMBB010000251         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_122173, whole genome shotgun sequence.
ACCESSION   DATMBB010000251 DATMBB010000000
VERSION     DATMBB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122173"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VNL92_05420"
     CDS             <1..602
                     /locus_tag="VNL92_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HWO94186.1"
                     /translation="MPTQSRQARRYRVIAIGASTGGPGAVVEILRALPFDFPLPVLLV
                     LHIGEPFGAAFADWLAGRTDRSVSYARGGELLSSSAGQVLMAPPGRHLVVRGDRLWLT
                     NDAERHSCRPSVDVLFESLAQEVGAAAALCLLTGMGKDGAAGLLAARKAGALTIAQDE
                     ATCVVYGMPREAVRLGAAERILPLQDIGPALASLGRHGQ"
     gene            599..>2137
                     /locus_tag="VNL92_05425"
     CDS             599..>2137
                     /locus_tag="VNL92_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWO94187.1"
                     /translation="MSDSVLIIDDSLTVRMDLADAFEAAGFRVRLSASAAQAREALAS
                     GPVDVVVLDVLLPDADGVELLRELRATTATSSAVILMLSTEAEVKDRVRALKTGADEY
                     VGKPYERLQLVSRAKELRRMRQTEMAEGRLTILVIDDSATFREGLQEALEGVGYSVIT
                     AASGEEGLRAAADRLPHAVIVDGLLPGMDGASVIRRIRLDAALRTIPCILLTASEEAG
                     AELRALDSGADAFVRKVESVEVILARVGAVLRRTSASPPREGTSSLLGPKRILAVDDS
                     RTYLHELAAALREEGYDVVLAHSGEEALELLAVQRVDCVLLDLLMPGLGGQETCRRIK
                     AAPGVRDVPVIMLTALEERGAMIEGLLAGADDYIPKSSDFEVLRARVRAQMRRRQFED
                     ENRRIREELLRRDLEARSAKELAEMRAVLLQELERKNRELEAFSYSVSHDLRAPLRSI
                     DGFSQALLEDYSQVLDERGRDYLRRVRGSAQRMAELIDDLLQLSRVSRAELRRAPVNL
                     SEMAS"
BASE COUNT          335 a          689 c          749 g          364 t
ORIGIN      
        1 ccatgccgac tcagtcccgc caagccaggc ggtaccgagt catcgccatc ggtgcctcta
       61 cgggaggccc cggcgccgtg gtcgaaatcc ttcgggcgct tccgttcgat ttcccgcttc
      121 ctgtgctgct cgtgctccac atcggcgaac ccttcggtgc cgcgtttgcc gattggctgg
      181 cgggccgaac ggatcgaagc gtttcctacg ctcggggagg cgagcttctt tcctcctccg
      241 ccggacaggt gctgatggcg ccgccaggtc gacacctcgt ggtccgcggc gaccgactgt
      301 ggctcacgaa cgacgccgaa cgccattcgt gccggccgtc agtcgacgtc cttttcgaat
      361 cgttggcaca agaagtcggt gcggcggcgg ccctatgcct gctgacgggc atgggcaagg
      421 acggggccgc agggctgctc gcggcgcgca aggcgggagc cctcaccatc gcgcaggacg
      481 aggcgacgtg cgtggtgtac ggcatgccgc gcgaggcggt gcggctcggg gcggccgagc
      541 gcattctacc gctccaggac atcggccccg cgttggcgtc gcttggaagg cacggccaat
      601 gagcgattcg gtgctcatca tcgatgacag cctgaccgtc cggatggatt tggcggacgc
      661 gttcgaggca gcgggctttc gcgtgcgttt gagcgcctct gccgcacaag cgcgcgaggc
      721 tttggcgagc ggcccggtcg acgtcgtggt cctggacgta ttgctgcccg atgccgatgg
      781 ggtggagttg ctccgagagc tgagagccac aactgcgacg tcttcggccg tcatcctcat
      841 gctgtcgacc gaggccgaag tgaaggatcg cgttcgggcg ctcaagacgg gcgccgacga
      901 gtacgtcggc aagccctacg agaggttgca gctcgtctcg cgggcgaaag agctccggcg
      961 catgcggcag accgagatgg ccgaaggtcg tctcaccatc ctcgtcatcg acgacagcgc
     1021 cacgtttcgc gaagggttgc aagaagcgct cgaaggggtc ggctacagcg tcatcacggc
     1081 ggccagcgga gaagaggggc ttcgtgccgc tgctgaccgc cttccccatg ccgtcatcgt
     1141 ggacggactg ctgcccggaa tggacggcgc atcggtgatt cgacgcattc gcctcgacgc
     1201 cgccctgcga accataccct gcattcttct caccgcatcg gaagaagcgg gtgccgagct
     1261 gcgggccctc gacagcggcg cggacgcctt cgtccgaaag gtggagagcg tcgaggtcat
     1321 tctcgcccgc gtcggcgccg ttctccggcg gacctccgcg agtcccccga gggaaggcac
     1381 gtcgagcctg ctcgggccca agcggatcct ggcggtcgac gacagcagga cctacctcca
     1441 cgagctcgcg gcagccttgc gcgaggaggg ctacgacgtg gtgctcgccc actccgggga
     1501 ggaggcgctc gagctgctcg cggtgcagcg ggtcgactgc gtgctgttgg acctgctcat
     1561 gcccggcctt ggcggacagg agacctgccg ccggatcaag gcggctcccg gcgtccggga
     1621 cgtgccggtc atcatgctca ccgcgctcga ggaacgcggc gcgatgatcg aggggctgct
     1681 cgcgggcgcc gacgattaca ttcccaagtc gagcgacttc gaggtgctcc gcgctcgcgt
     1741 gcgcgcccag atgcggcgtc ggcaattcga ggacgagaac cggcggatac gggaggagct
     1801 gttgcggcgc gacctcgagg cgcgctcggc aaaagagctc gcggagatgc gggccgtgtt
     1861 gctccaggag ctcgagcgca agaacaggga actcgaagct ttcagttatt ccgtgtcaca
     1921 cgatcttcgg gctccccttc gcagcatcga cgggttcagc caggcgttgc tcgaggacta
     1981 cagccaggtt ctcgacgagc gagggcgcga ctatttgcgg cgtgtgcgcg gctcggccca
     2041 gcgaatggcc gagctcatcg acgatttgct tcagctgtcg agggtgagcc gcgccgagct
     2101 caggcgagcg ccggtcaatc tttcggagat ggcgtcc
//
LOCUS       DATMBB010000252         3078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_42587, whole genome shotgun sequence.
ACCESSION   DATMBB010000252 DATMBB010000000
VERSION     DATMBB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42587"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1441)
                     /locus_tag="VNL92_05430"
     CDS             complement(149..1441)
                     /locus_tag="VNL92_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWO94188.1"
                     /translation="MSFPRRRLRTSAVGLLAALLIAAASACGDDDSDAGTATAGNSEQ
                     QSASTAPAAQDPFDHLRVLADDIGPRPAGSDAERRAAEYLRDEYASAGYETELQTFTF
                     SAPPSALVASPSGAIAFTTAFSGSPSGDVTGPLVYVGLARPADVPAAGLAGAIALAER
                     GEIFFQDKAANVAAAGARALIVINNEAGPLGGEVESSPIPVVGAPDSERDHLLALAEA
                     GDTVHVVLEPADTMTSQNVVARPPDGRCVVVVGAHYDSVPQGPGANDNASGAGVALAI
                     AEERAERGDLDGACYVSFGAEEVGLHGSQHFVQALSAAQLAGLRAMLNLDMVGAGGSW
                     RLIGSPEYQELGQRLAGELGIAAEPWELPPQFGSDHQAFVEAGVSSLFLHRFEDPNYH
                     TPNDTSQAVDPGLLREALAFGHAFVAEVLTEAPVLASR"
     gene            complement(1485..1889)
                     /locus_tag="VNL92_05435"
     CDS             complement(1485..1889)
                     /locus_tag="VNL92_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94189.1"
                     /translation="MADRGDRVEREIEDILRKIDDFPTEAARRRERRGREAPQASLGQ
                     RLARRIGRITPAQLMIASMVLVFAGFLIVDPIEANVAKYMIIAGLILFFSSFVLSFRP
                     ARRQQAPKRWRGRVIEPDRSSGRGIRGWWGRR"
     gene            complement(1969..2457)
                     /locus_tag="VNL92_05440"
     CDS             complement(1969..2457)
                     /locus_tag="VNL92_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94190.1"
                     /translation="MTSQPQRHLLVLRGRAGARQRELAMWLASELPGPTAVIPQDAFH
                     TRWVLKPGPDRARESEMGARIGKLVAISYMREGYSVVVEGAYAPEDPGQESGLSELIS
                     IARTLPARTYVIELRESRYESQAPSAVASAIRADDVVDLTDEPIGSAAARIVAQLLRV
                     TP"
     gene            2522..>3078
                     /locus_tag="VNL92_05445"
     CDS             2522..>3078
                     /locus_tag="VNL92_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWO94191.1"
                     /translation="MEADGVEEREQAEHAPWLIDMHVHSTLGSADSNLTPDHLVRRAR
                     VLGLAGAALTEHYRVWPDEEVARAQAPDLALLPAAEWSTDLGHILCFGIERHVSGMSR
                     AEDVREYVLERRGFMVAAHPFRYAFTTQERHAYLGTRGRMTAEQAAEAPVFRLVDEIE
                     VCNGGCTDEENLFALQVAHLLGKRG"
BASE COUNT          523 a          998 c         1045 g          512 t
ORIGIN      
        1 gcgaatcgat ctcttgcgcc tcgccgccgt catcatcgtc gtcgccacag gcgacgagca
       61 aggcaagcga aacgatgagg gtagactgaa gaagtagccg gtagctccgg ccgcgccatc
      121 tcaacacggt cggatgctaa caaacgcgct accgcgaagc aaggacgggg gcttctgtca
      181 ggacctccgc cacgaatgcg tgaccgaacg ccagcgcctc cctcagcagg cctgggtcca
      241 ccgcttggct cgtgtcgttc ggcgtgtgat agttgggatc ctcgaagcga tgaaggaaga
      301 gggacgatac acctgcctcc acgaacgcct gatgatccga cccgaactgc ggcggcagct
      361 cccatggttc ggcggcgatg ccgagctccc ctgcgaggcg ctggcccagc tcctggtact
      421 ccggcgagcc aatgagccgc cagctccccc cggccccgac catgtccagg ttcagcatcg
      481 ctcggagtcc ggcgagttgg gccgcactca aagcctgcac gaagtgctgg ctaccgtgga
      541 ggccgacttc ctccgcgccg aacgatacgt agcaggcgcc gtcgagatcg ccgcgctccg
      601 cgcgctcctc cgctatcgcc agggcgacgc ccgcgccgga cgcgttgtcg ttcgcgcccg
      661 gaccttgcgg gacggaatcg tagtgagcgc cgacgaccac cacacaccgg ccatcgggcg
      721 gacgggcaac gacattctgc gaagtcatgg tgtcggctgg ctcgagcacg acatgtactg
      781 tatcgcccgc ctcagcgagc gcgaggaggt ggtcgcgctc ggagtccggc gcgcctacca
      841 cgggaatcgg cgaggactcg acttcgccgc ccaggggacc ggcctcgttg ttgataacga
      901 tgagtgctcg ggcccctgca gcggcgacgt tggctgcctt gtcctgaaag aagatctcgc
      961 ccctctccgc gagcgcgata gcgcccgcca acccggccgc cggcacatcg gctgggcggg
     1021 cgagccccac gtagacgagc ggacctgtca catcgccgct gggcgagccc gagaatgcag
     1081 tcgtgaacgc gattgctccg gagggtgacg ccacgagggc gctggggggc gcggaaaacg
     1141 tgaaggtctg gagctcggtc tcgtagccgg cggaggcgta ctcgtcgcgc aggtactccg
     1201 ctgcgcgacg ttcggcatcg gaccccgccg gacgcggacc gatgtcgtcg gcgagcacgc
     1261 gaaggtggtc gaaagggtct tgtgctgccg gtgctgtgga tgctgattgc tgctctgaat
     1321 tgccagctgt cgcggtcccg gcgtcggagt cgtcatcccc gcatgcggag gctgcggcga
     1381 tgaggagggc ggccagcagc ccgaccgcgc tcgtcctcag ccggcgacga gggaagctca
     1441 tggtgcgcac atgatggaaa cggcggcact tggcctggcg gatgttagcg gcgcccccac
     1501 catcctcgga tgccgcgtcc agaagaacga tcgggctcga tgacgcggcc acgccagcgc
     1561 ttcggcgcct gctgccggcg tgccggccgg aaggagagga cgaaactcga gaagaacagg
     1621 atgaggcctg cgatgatcat gtacttcgca acgttcgcct caatcggatc cacaatgagg
     1681 aagccggcga acacaagcac catgctggca atcatgagct gcgccggcgt gatgcgaccg
     1741 atgcgccggg cgagccgttg gccgagcgag gcttggggag cctcccgtcc ccggcgttcg
     1801 cgcctacggg ctgcctcggt cgggaagtcg tctatcttgc ggaggatgtc ctcgatctcg
     1861 cgttcgacgc ggtcgcctcg gtcagccatc gagcagctcc ttaggtgcaa gtgcagcttg
     1921 gctcattcta gcgccaggag ggattgcgag tccacgtaca ttgcggacct atggcgtcac
     1981 gcgcaacagc tgcgcgacaa tacgcgccgc cgccgatccg atcggttcgt ccgtcaagtc
     2041 gacgacatcg tcggcccgta tcgcagaggc gactgctgat ggtgcttggg actcgtatcg
     2101 cgattcccgg agctcgatga cataggtccg cgctggcagc gtccgtgcaa tcgagatcag
     2161 ttccgaaagg ccagactctt gccctgggtc ctcgggggcg taagctccct cgaccacgac
     2221 gctgtagcct tccctcatat agctgatcgc gacgagcttg ccaatgcgcg cccccatctc
     2281 cgactcccgc gcccggtccg gcccgggttt cagaacccac cgtgtgtgga aggcgtcctg
     2341 ggggatgacg gccgttggtc cgggcaattc cgaggcaagc cacatcgcga gctcgcgctg
     2401 ccgtgcccct gcgcgaccgc gaaggacgag gaggtggcgt tgaggttggc tcgtcacgat
     2461 cttctccgct tcgtgttgcg cagcgagtat agccgaatcc cacatgctag acttcaggag
     2521 gatggaagcg gacggcgtgg aggagcgcga acaggcggag cacgcgccgt ggctcatcga
     2581 tatgcacgtg cacagcacgc ttggttcggc cgatagcaac ctgacgcccg accacctggt
     2641 gcggcgcgcg cgggtgcttg ggctcgccgg agccgccctc acggagcact accgcgtgtg
     2701 gccggacgag gaagtcgcgc gggcgcaggc tccggacctc gcgctgttgc ccgccgcgga
     2761 atggtcgacc gatctggggc acatcctgtg cttcggcatc gagcgtcacg tgtccggcat
     2821 gtcgcgcgcc gaggacgttc gcgagtacgt gctggagaga cgggggttca tggttgctgc
     2881 tcaccccttt cgctacgcgt tcacaacgca ggagcggcac gcatatctcg ggacgcgcgg
     2941 ccggatgacg gcggagcagg ctgccgaggc gcccgtgttt cgtctcgtcg acgaaatcga
     3001 ggtgtgtaac ggcggttgta ccgacgagga gaatctcttc gcgcttcagg ttgcccatct
     3061 gctcggcaag cgtggcat
//
LOCUS       DATMBB010000253         2509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_519019, whole genome shotgun sequence.
ACCESSION   DATMBB010000253 DATMBB010000000
VERSION     DATMBB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519019"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..968)
                     /gene="prfB"
                     /locus_tag="VNL92_05450"
                     /pseudo
     CDS             complement(1..968)
                     /gene="prfB"
                     /locus_tag="VNL92_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158066129.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(965..1678)
                     /gene="nfi"
                     /locus_tag="VNL92_05455"
     CDS             complement(965..1678)
                     /gene="nfi"
                     /locus_tag="VNL92_05455"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144227.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HWO94192.1"
                     /translation="MTSASARHAWDLRPRDARELQLQLAPLVETEGEPGNVRIVAGVD
                     VAAPRSARMARVALVLLSFPTLEEVGHVALELGVQFPYVPGLLSFREAPLILTALERL
                     GTPPDLLFVDGQGVAHPRRFGIASHIGLLTGIPTIGCAKSRLVGQYEMPGEHPGDRSM
                     LMDDGQRIGTVVRTKAGARPLFVSPGHRIGFEEATRWVLACCRGHRLPEPTYLADRMS
                     KASVLRDSRSPGEGWENPQ"
     gene            1774..2208
                     /locus_tag="VNL92_05460"
     CDS             1774..2208
                     /locus_tag="VNL92_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWO94193.1"
                     /translation="MATETRIEEEILNLLFRLGRAFTRRRHTPNGEAELSMLQLQALL
                     FIRERENCSMSELANEFHIGLPSATALVDRLVRAGLLSRAEDPNDRRVTRVRLTNAGK
                     ERVRRLFEQEVAETRAIVDRLPGEDREALVRIMTHVLRELEA"
     gene            2301..2376
                     /locus_tag="VNL92_05465"
     tRNA            2301..2376
                     /locus_tag="VNL92_05465"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2334..2336,aa:Phe,seq:gaa)
BASE COUNT          425 a          827 c          815 g          442 t
ORIGIN      
        1 agcttctgat ttggttaccc catccggcgc tcacgtgctc gcccttcacg cgcgcctgct
       61 cctccgcacg gcgctccagc tccagttcca ggagccgcgc atgaagcacc ttcatggcgc
      121 tctcgcggtt gcgaccctgc gatcgctcgt tctgacatgt gactacgatg ccagtcggta
      181 gatgggtgat gcggatggca gtcgagttct tctggacgtt ctgccccccg tgcccgctgg
      241 cacggaacac atcgatccgc aggtcgtcgg cgtcgatgtg cacttctgcg gcctcatccg
      301 cttcgggcat cacctcgacg agggcgaacg atgtgtggcg ggcatgcgcc gaatcgaagg
      361 gagacagccg tacgaggcga tgcacacccc gctcggcgcg caggaagccg taggcatatt
      421 ctccgctgaa ctcgacggtg gcgctcttga tccccacctc ctctccgggc gtgatgtcga
      481 tgacatcggt ccggtagccg cgcttctcgc cccagcgcag gtacatgcgc agcagcatct
      541 ccgcccagtc ctgcgactcg gtccccccgg cgccggcatg gatggcgaga atagcggatc
      601 ggtcgtcgta cgggccggag aacgacaagg cgaactcgcg ctcctcgacc tcgcgtcgga
      661 gcgcatcgta gtcgcgttcc gcctcggcca ggaacgcctc atcgccctcc gccgccgcga
      721 gcccgacgag gtcgcgcagg tcaccggcgc gccgttcgat gtcgttccag acatcgatgg
      781 tcttgcggag ggaggcgagc tgctgcatgg cgtcgcgagc ggctgcttgg tcgtcccaaa
      841 agcccggcgc gctggtacgc gcggcgagct cttcggcctc tcgctccttt ccggctatgt
      901 caaaggcgca ccatggtatc gcggatgtgt tcgagaaggg actcggtctc gtcccgaagg
      961 tcgctcattg agggttctcc catccttccc ccggggatcg cgagtcccgg aggacgctgg
     1021 ccttgctcat acgatcggcg aggtacgtcg gctcgggcaa ccgatgccct cggcagcacg
     1081 ccaagaccca ccgcgtcgct tcctcgaacc cgatgcgatg tcccggcgag acgaacagcg
     1141 ggcgcgcccc tgccttcgtc ctgacgacgg tcccaatccg ctgtccgtcg tccatgagca
     1201 tcgaccggtc gcccggatgt tcccccggca tctcgtattg tcccacgagg cgcgactttg
     1261 cgcatccgat cgtcgggatg ccggtaagca gcccgatgtg gctggcgatc ccgaaccgcc
     1321 gtgggtgcgc aacgccctgg ccatcgacaa agaggaggtc gggcggcgtc cccagtcgct
     1381 ccagcgccgt gaggatgagt ggcgcttcgc ggaacgagag caggccggga acgtagggga
     1441 actggacgcc gagctcgagc gccacgtgtc cgacctcctc gagcgtgggg aacgagagca
     1501 gcacgagggc aacgcgcgcc attcgggcgg atcgaggtgc cgcgacgtcc acaccggcga
     1561 cgatgcgcac gttccccggc tcgccctccg tttcgaccaa tggagcgagc tgcagctgca
     1621 gctcgcgcgc atcgcgaggg cgaaggtccc aagcgtggcg tgctgacgcg gacgtcatag
     1681 cggtagagac ggaatcttcg tcccgtgata cactacgcat gtatcaacca attcctgccg
     1741 cggctcccgc agaaaataac gatcggaagg gcgatggcga ccgaaacccg tatcgaagaa
     1801 gagattctca acttgctgtt ccggctggga cgggcgttca cgcgccgccg tcataccccc
     1861 aacggcgaag cggagcttag catgctgcag ttgcaggcgc tgctcttcat cagagagcgc
     1921 gagaattgca gtatgagcga attggcgaat gagtttcaca tcggcttgcc ttcggccacg
     1981 gcgctggtcg atcggcttgt acgcgcaggc ttactgagcc gggccgagga ccccaacgac
     2041 cggcgcgtga cgcgcgtgcg gttgacgaac gccgggaagg aacgcgtacg ccggctcttc
     2101 gagcaagagg tggccgagac gcgggcgatc gtcgatcgac tgccgggcga ggaccgggag
     2161 gccttggtcc gcatcatgac acatgtcctg cgagagttgg aggcgtaggc gaaggcctca
     2221 gccttcctcg ccttgaccgg gcgggaacat cccgcctatg ctgaaattcg agttccccgt
     2281 ccaagtttgc acgtgccgct gctggggtag ctcaggtggt agagcacacg actgaaaatc
     2341 gtggtgtcgg cggttcgact ccgcccccca gcaccagttt ttgcatctga gaggctcccg
     2401 tttaggagcc tcttggtggg tccgagcgcc gaggcactgt gtcgcaggtc ccggaggcgc
     2461 gcccagacgg cagcggcctt gcacccacca tatctaccaa gcggggatc
//
LOCUS       DATMBB010000254         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_925, whole genome shotgun sequence.
ACCESSION   DATMBB010000254 DATMBB010000000
VERSION     DATMBB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1683
                     /locus_tag="VNL92_05470"
     CDS             166..1683
                     /locus_tag="VNL92_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HWO94194.1"
                     /translation="MARRAASTAIIEWIEKHCRVPEGRDVGKPVTLRPWQRREIKKIY
                     DNPHGTRRAILSFGRKNGKTAAAAFLLLVHLCGPKARPNSQLFSAAQSREQAAILFGL
                     AAKIVRMSPTLVDYVVVRDTAKQLYCPELGTLYRALSAEASTAYGLSPVFVVHDELGQ
                     VRGPRSELFEALETAMSAHESPLSVIISTQAPTDGDLLSILIDDALQGHDKRVVCSLY
                     TAPPELPPFDRKTIKLANPAFGDFQNADEVMAMAADAERMPSREAEFRNLILNQRVEV
                     NNPFISRAVWEACGGPVAEYTGGVYIGLDLSETTDLTALVMVSAVDGAWHVKSKFWLP
                     EEGLRERARRDRVPYDLWHDQGFLDVTPGRSIEYEWVAQRMREIFDRYDVRRVGFDRW
                     NWRHFKPWLVKAGFSEEELKRFEEFGQGTQSMSPALRTLEAALVAGKIRHGGHPVLTM
                     CAANAVVNSKDAANRKLVKAKSSGRIDGMVALAMAIGVAASEDLEPQELSFWEVA"
     gene            1684..2946
                     /locus_tag="VNL92_05475"
     CDS             1684..2946
                     /locus_tag="VNL92_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HWO94195.1"
                     /translation="MAWWPFRRRSGRKAGDGTLELFREIFGYPETKAGQRVNSTTALQ
                     VATVLACARVLADGVAQVPLKLFKESADGRMRLPAKDHHLYWLLFRRPNPWQTSFQYR
                     ETLMLHLVLCGNHFSFINRVRRRIVELIPFEPGQVTVKRDERTWARTYEVRATESGAK
                     QVFPQEAIWHISGPSWSGWLGLEPVLLAREAIGLSMAIEAQQAGFYARGAQTGGVYSI
                     DGTLTDEQYKKLRDWIEKEHGGAANAWRPMVLDRGAKWLQQTLSGHDAQTLEQRKYQV
                     EEICRAMRVMPIMVGHSDKTSTYASAEQMFLAHVVHSLAPWYERLEQDIDANLLTDED
                     VRNGLYAKFIEEGLLRGALTDTAEYLSKLTGGPIMSRNEARAKLDMNPVDDPEFDKPL
                     TPLNMGGDQEPEPEPSSDATTTDAEQET"
     gene            2949..>3515
                     /locus_tag="VNL92_05480"
     CDS             2949..>3515
                     /locus_tag="VNL92_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HWO94196.1"
                     /translation="MERFACALELKFADSDTKEMTFSGYGAVFGNVDSYGDVIQKGAF
                     RETLREAKKSKQFPAMLLQHGGWGVTSEDFTPIGIWTDMEEDDVGLRVEGKLADTARG
                     REAYTLLKMTPRPAINGLSIGYRAKEYSLGTKPEEPRRTLKKVDLLEVSLVTFPANPK
                     ARVSAVKSIEQLSTLAEAERYLRDACGLS"
BASE COUNT          646 a         1042 c         1232 g          595 t
ORIGIN      
        1 tcacgaagag atcgatgtcg agcagtacga ccggctgctc aagatgcagg agcgtgaggg
       61 ccgggcgatc tcgtcactcg cgacccggat gcgcatatcg cagcagtcga cgctcgatcg
      121 agagaagcgc aagccggtca agccgcagcg gaagccctgg gagtaatggc gcgcagagcg
      181 gcatccaccg cgattatcga gtggatcgag aagcattgcc gcgttccgga gggtcgggat
      241 gtaggcaagc cggtgacgtt gcggccgtgg cagcggcgcg agatcaagaa aatctatgac
      301 aacccgcacg ggacgcggcg ggcgatcttg agcttcggcc ggaagaacgg caagacggcg
      361 gcagctgcct tcctgctgct tgtgcatctt tgcgggccga aggcgcggcc gaacagccag
      421 cttttcagcg cggcgcagtc gcgggagcag gcggcgattt tgttcgggct cgcggcgaag
      481 atcgtgcgca tgtcgccgac actggtcgat tacgtcgtcg ttcgcgatac ggccaagcag
      541 ttgtattgtc ccgagctcgg gacgttgtat cgcgcgttgt cggctgaggc ctcgacggcc
      601 tatggtctct cgcccgtctt cgtcgtgcac gatgagctcg ggcaggtgag aggccctagg
      661 tcggagcttt tcgaggcgct cgagaccgcg atgagtgcgc atgagagtcc gctctcggtc
      721 atcatttcga cgcaggcgcc gaccgatggc gacctgctct cgatcctgat cgacgatgcg
      781 ctgcagggcc acgacaagcg tgtggtgtgc agcctgtaca cggcgcctcc cgaactgccg
      841 ccgttcgacc gcaagacgat caagctcgcg aatccggcgt tcggcgattt ccagaacgcc
      901 gatgaagtca tggcgatggc ggcggatgcc gagcgcatgc cgtcgcgcga ggcggagttt
      961 cggaacctga tcctgaatca gcgcgtggag gtgaacaatc cgttcatctc gcgtgctgtg
     1021 tgggaggcgt gcggcgggcc ggtggccgag tacacgggcg gcgtatacat cggtctcgat
     1081 ctgtccgaga cgaccgacct tacggcgctc gtgatggtgt cggcggtcga tggtgcatgg
     1141 cacgtgaagt cgaagttctg gctgcccgag gagggcctgc gcgagcgtgc gcgtcgggat
     1201 cgcgtgccgt acgacctctg gcatgaccaa ggctttctcg acgtcacgcc cgggcgatcc
     1261 atcgagtacg agtgggtcgc gcagcgcatg cgcgagatct ttgaccgcta cgacgtgcgg
     1321 cgcgtcggat tcgaccgttg gaactggcgt cacttcaagc catggctcgt gaaggccggt
     1381 ttctccgagg aggaactcaa gcggttcgag gagttcggtc aagggacgca gtcgatgtcg
     1441 ccggcccttc ggacgcttga ggccgcgctc gtcgccggga agatccggca tggcgggcac
     1501 cccgtgctga cgatgtgcgc ggccaacgca gtggtgaatt cgaaggacgc cgcgaatcgc
     1561 aagctcgtga aagcgaagtc aagcggaagg atcgacggaa tggttgcact ggcgatggcg
     1621 atcggggtcg cggcgagcga ggacctcgag ccgcaggagc tatcgttctg ggaggtcgcg
     1681 taggtggcat ggtggccgtt tcgacggcgc agcggtcgca aggctggcga cggcacgctt
     1741 gagctcttcc gcgaaatctt cggctatccg gaaacgaagg cgggacagcg cgtcaactca
     1801 acgacggcac tccaggtcgc cacggtgctg gcgtgcgccc gcgtcctcgc cgatggtgtc
     1861 gcgcaggtcc cgctcaaact cttcaaagag tcggcggacg gtcggatgcg gctgccggcg
     1921 aaggatcatc atctctactg gctgctgttc cgtcgcccga atccttggca gacgagcttc
     1981 cagtatcgcg agacgctgat gctgcacctc gtgttgtgcg gcaatcactt ctcgttcatc
     2041 aaccgggtgc gcaggcggat cgtcgagctg attccgttcg agcccgggca ggtcaccgtc
     2101 aagcgagacg agcgtacctg ggcgcgaacg tacgaggtgc gcgcgacgga gtctggggcg
     2161 aagcaggtgt tcccgcagga ggcgatctgg cacatcagcg ggccctcgtg gtcaggctgg
     2221 ctcggcctgg agcccgtgct gctcgcgcgc gaggcgatcg ggctctcgat ggcgattgag
     2281 gctcagcagg ccggttttta cgcgcgcggc gcgcagaccg ggggcgtcta ctcgatcgat
     2341 ggcacgctga ccgacgagca gtacaagaaa ctccgcgact ggatcgagaa ggagcacggg
     2401 ggcgcggcga atgcctggcg gccaatggtc ctcgaccgag gcgcgaagtg gctccaacag
     2461 acgctctccg gtcacgacgc gcagacgctc gagcagcgca agtaccaagt cgaggagatc
     2521 tgccgggcaa tgcgcgtcat gccgatcatg gtcggacact ccgacaagac gtcgacctac
     2581 gcttcggccg agcagatgtt ccttgcgcac gtcgtgcact cgctcgcgcc ctggtacgag
     2641 cggctcgagc aggacatcga tgcgaacctg ctcaccgatg aggacgtgcg caacggtctg
     2701 tacgcgaagt tcatcgagga agggctcctc cgcggcgcgc tgacggacac cgccgagtat
     2761 ctcagcaagc tgactggcgg gccgatcatg agccgtaatg aggcccgcgc caaactcgac
     2821 atgaatccgg tggacgatcc cgagttcgat aagccgctga cgcccttgaa catgggcggc
     2881 gaccaggagc cggagccgga gcctagcagc gacgcgacga cgactgatgc cgagcaggag
     2941 acgtaaagat ggagcgattc gcttgcgcgc tggagctgaa gtttgccgac agcgatacga
     3001 aggagatgac gttctccggc tacggcgccg tgttcggcaa cgtcgacagt tacggagacg
     3061 tcatccagaa gggcgcattc cgcgagacgc tccgcgaggc gaagaaatcg aagcagttcc
     3121 cggccatgct gctgcagcac ggcggctggg gcgtgacgtc ggaggacttc acgccgatcg
     3181 gcatctggac cgacatggag gaggacgatg tcggcctgcg cgtcgagggc aagctcgccg
     3241 acactgcgcg tggccgcgag gcgtacacgc tgctcaagat gacgccgcgg ccggcgatca
     3301 acggtctctc catcggctac cgggcgaagg agtactcgct cggtacgaaa ccggaggagc
     3361 cgcggcgcac cctgaagaag gtcgatctcc tcgaggtgtc gctggtgact ttcccggcga
     3421 accccaaggc gcgcgtgagc gcggtcaagt ccatcgagca actttccacg ctcgccgagg
     3481 cggagcgcta cctgcgtgat gcttgtggcc tctcg
//
LOCUS       DATMBB010000255         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_86268, whole genome shotgun sequence.
ACCESSION   DATMBB010000255 DATMBB010000000
VERSION     DATMBB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86268"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VNL92_05485"
     CDS             <1..765
                     /locus_tag="VNL92_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO94197.1"
                     /translation="EGPAFDVIVPSLPGYAFSEPPKQPGMHLGRIAERMHLLMRDLGY
                     ERYGVQGGDWGAAVGSRLAQQHPEAVIGLHLNFAIAPPPPLEELSDDERAALRDAEMW
                     RMQETAYAMLQGTKPQTLAYALVDSPTGLLAWILEKFWAWSDHGDDLWQTFEKDAVLA
                     NVSLYWFTNTVLSAARTYYEARRESPAMAVTAGRVEVPTAFANFPAEPFRSPRAAIER
                     AYNLVRWTDQQKGGHFAAMEQPELFAKDVAEFFASL"
     gene            complement(785..1759)
                     /locus_tag="VNL92_05490"
     CDS             complement(785..1759)
                     /locus_tag="VNL92_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94198.1"
                     /translation="MQVSPNVRAVQVPDDNPMHPQFTTIYLVGRGQVFTVDSGEDMDR
                     YRWMLRGYLAATEKAEIALSGITHHHIDHSANLGWLRDSFGVELYVHAEGRPLLGERL
                     PQNGVHVLRDGDSIEVGGVRLAVLHTPGHSVDSVCYYLESEGVLFSGDTILGSTTTTI
                     GDLGAYMASLRRLRGLPNLRMICPGHGPIIEDPLSYIDQYVAHREERERQILSVLREG
                     GEVTSWEIMLRLYPEIDERLRRAADNNVRQHLAKLEAEGLVRAYPGVPKPKSEEQILK
                     ERAEEEERRSIIAKAREYEEQARRRMAYLQENPPLDEWEEMPRYELVR"
     gene            complement(1762..2190)
                     /locus_tag="VNL92_05495"
     CDS             complement(1762..2190)
                     /locus_tag="VNL92_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009952367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HWO94199.1"
                     /translation="MAQKRYFEDVEVGDEIGPVIENCSEVQLFFFSAATNNGHRIHYD
                     LPYAKTEGHPTILVHGPLQTALMAKTVLDWAGPGARLVRIQLQNRGSAYPHEDLYFGG
                     VVTDKRQESGRALVDCDLYERKADGTVIMPGTATVSLPRR"
     gene            complement(2194..>2384)
                     /locus_tag="VNL92_05500"
     CDS             complement(2194..>2384)
                     /locus_tag="VNL92_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO94200.1"
                     /translation="GEDTEFYEPVYPGDVISSVTRIAAIEEKTGRSGRFVLVTRETVY
                     TNQDGVVVARSRNSSIVR"
BASE COUNT          396 a          803 c          759 g          426 t
ORIGIN      
        1 gagggccccg cattcgacgt catcgtcccg tcgctccccg gatatgcgtt ctcggaacca
       61 cccaagcagc ccggcatgca cttggggcgc attgccgagc gcatgcatct gctcatgcgt
      121 gacctcggtt acgagcgcta cggcgtgcag ggcggcgatt ggggtgccgc ggtcggctca
      181 cgcttggccc agcagcatcc ggaggcggtc atcggcctgc acctcaactt cgccattgcg
      241 cctccgccgc cgctcgagga attgagcgac gacgagcgtg ccgccctacg cgacgccgag
      301 atgtggcgta tgcaggagac ggcatacgcc atgctccaag ggacgaagcc gcagacgctc
      361 gcctatgcct tggtcgattc gccgacagga ctgctggcct ggatactcga aaagttctgg
      421 gcttggagcg atcacggcga cgacctgtgg cagacgttcg agaaggacgc ggtacttgca
      481 aacgtcagcc tatactggtt cacgaacacc gtgctgtccg cggcgcgcac ctactacgaa
      541 gcgcgacgtg aatcgcccgc gatggccgtg acggcaggcc gcgtcgaggt gccgaccgcg
      601 ttcgcgaatt tccccgcgga gccgttccgg tcgccgcgcg ctgccatcga acgggcctac
      661 aacctcgtgc ggtggaccga ccagcagaag ggcggccact tcgccgcgat ggagcaaccg
      721 gagctcttcg ccaaggacgt cgccgagttc ttcgcttcgc tctgagcagc ccgcgcgtac
      781 tctgctagcg caccagctcg tatcgcggca tctcctccca ctcgtcgagc ggcggattct
      841 cctgcaggta ggccatgcgc cggcgggcct gctcctcgta ttcgcgcgcc ttcgcgatga
      901 tgctccggcg ctcctcctcc tccgcgcgtt ccttgaggat ctgctcctcg ctcttcggct
      961 tgggcacacc cggatacgcg cggaccaagc cctccgcctc cagcttcgcg agatgctgac
     1021 gcacgttgtt atcggcggcg cggcgtagcc gctcgtcgat ctcagggtag aggcgcagca
     1081 tgatctccca gctcgtgacc tccccgccct cccgcagcac gctcaggatc tgccgctcgc
     1141 gctcctcccg gtgcgcaacg tactggtcga tgtacgagag gggatcctca atgatcgggc
     1201 catgtccggg gcaaatcatg cgcaggttcg gcaggccgcg caggcgccgc agactggcca
     1261 tgtaagcacc gaggtcgccg atggtcgtcg tggtggagcc gagaatcgta tcgccggaga
     1321 agagcacgcc ctcgctctcg aggtagtagc agaccgagtc gacgctgtgg cccggcgtat
     1381 ggagcacggc gaggcgaacg cccccgactt cgattgaatc gccgtcgcga agaacgtgca
     1441 caccgttctg gggaaggcgc tcgccgagaa gaggccggcc ctcggcatga acgtagagct
     1501 cgaccccgaa agaatcgcga agccagccca ggttggcgct gtggtcgatg tggtggtgcg
     1561 taatccccga gagggcgatc tccgcctttt cggtcgctgc gagatagccc cggagcatcc
     1621 agcgatagcg gtccatgtcc tcgccggagt cgaccgtgaa cacctggcca cgccccacga
     1681 ggtagatcgt cgtgaattgg gggtgcatcg ggttatcgtc gggcacctga accgcccgaa
     1741 cgttggggct gacctgcatg gctagcgccg cggcaggctt acggtggcgg ttcccggcat
     1801 aatgaccgtg ccatccgcct tccgctcata gagatcgcag tcgacgaggg cgcgtccgct
     1861 ctcctgccgc ttgtcggtca ccacaccgcc gaagtagagg tcctcgtggg gataggcgct
     1921 gccccggttc tggagctgga tgcgcaccag ccgagcgccg ggccccgccc agtcgagcac
     1981 cgtcttggcc atgagcgctg tctgcagcgg gccgtgcacc agaatcgtcg ggtggccctc
     2041 cgtcttcgca taggggaggt cgtagtgaat gcgatggccg ttgttcgtcg ccgcggagaa
     2101 gaagaacagc tgcacctcgg agcagttctc aatcactggg ccgatttcat cgccgacctc
     2161 gacgtcttca aagtaccgct tctgggccat ccctcacctc acgatgctgg agttgcggct
     2221 tcgcgccacc acaacgccgt cctggttggt gtacaccgtc tctcgcgtca caaggacgaa
     2281 tcgcccggag cggccggtct tctcctcgat ggccgcaatg cgcgtgacgg acgagatgac
     2341 atcccccgga tacaccggtt cgtagaactc cgtatcctcg ccgc
//
LOCUS       DATMBB010000256         2903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_111488, whole genome shotgun sequence.
ACCESSION   DATMBB010000256 DATMBB010000000
VERSION     DATMBB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111488"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNL92_05505"
     CDS             <1..492
                     /locus_tag="VNL92_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HWO94201.1"
                     /translation="NLGWLRLPCEQKSDLAVRSGLHLQNGTPCTDDQIVLISDKLFAH
                     VVNSNLEVRTSVAINPETGAADEGKLFTYEALPRGTVLVWDITCRDPRHFQRTLQNAS
                     GDASVTVLDVVRKAEPFFEALGVGGMGTRGMGRLKVLSCCDASASAAALATGGLPNGE
                     ASR"
     gene            489..1043
                     /locus_tag="VNL92_05510"
     CDS             489..1043
                     /locus_tag="VNL92_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94202.1"
                     /translation="MSDGNKTIRDIDLACAEAGRRLAALGNRAKDAENTLNKALGVLE
                     EQGVYAMFLYLFAREERCGVDELCQLLGDTCDTDVPRQCGAELKDLESRLQNARRQEK
                     EQGRQQKPQQRGRSGRHQTEAGVSEITRLEEEIQKKKTEFFNKVESLANDLDQLLFAR
                     EVLHNALVYARYHAKARASAGERE"
     gene            1040..1942
                     /locus_tag="VNL92_05515"
     CDS             1040..1942
                     /locus_tag="VNL92_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94203.1"
                     /translation="MTWSLYRWTWQLESPLYVGMPPAGSLNRCRLYVPARALWGAVTA
                     ELARAKGASTPADYKRVGEQVSEHVRFTYLYPAEATGKDWRAWLPKYERGEGLVWERE
                     DRVGDPRSDRAFRTRLLSSRPGTAIDPGTETAADGSLRETECINEQWRDTDGRAGSRV
                     ALVGYLFICTANGAAASPIAASELEGLDTLFIGGDTRYGLGKLSRAFPGGWEEATSVF
                     GAAVDVAGPHPRIRSGRVLAHARPPRTGGREVFGQLELVAGWDDRNLVPQDTGPLLAP
                     GSSSGEELWWRLEPSGFWNPASIA"
     gene            1894..>2903
                     /locus_tag="VNL92_05520"
     CDS             1894..>2903
                     /locus_tag="VNL92_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWO94204.1"
                     /translation="METGAERLLESGEHRVNIFELHRNVLTDYRDFVRSFFLIADERA
                     REFVDRAIEQEQRLWPEPLVQLSPAYVPGPNVDELARDGLVAQETARIFSWPDGTTFR
                     LYRHQEEAIRKVLAGESIVVTSGTGSGKSLCYFLPIVDSLIRRPETGERVAALIVYPM
                     NALINSQFQALSGLKERYERALGRPFPVTFAKYTGDTPDSDRDDMRRKPPRILLTNYV
                     MAELLLVRPEDQRFLDRAGGGLRFLVFDELHAYRGRQGADVAMLVRRLKERCAEEGLV
                     HIGTSATMIARPEATREERCRSVADFAARFFGHPFSREQVIEETLVPFTQGGPPSAGE
                     LRS"
BASE COUNT          564 a          875 c          991 g          473 t
ORIGIN      
        1 aacctcggct ggcttcgcct gccctgcgag cagaaaagtg atttggctgt gcgcagcggt
       61 ctccatctcc agaacggaac cccgtgcacg gacgaccaga tcgtgctgat ctcggacaag
      121 cttttcgcac acgtcgtcaa cagcaacctc gaggtgcgca cctcggtcgc gatcaacccg
      181 gaaaccgggg ctgccgacga gggcaagctg tttacgtacg aggcgctgcc gcgcggaacc
      241 gtactcgtat gggacatcac ttgccgcgat ccacggcatt tccagcggac gcttcagaat
      301 gcgagcggcg acgcgagcgt cacggtgctg gacgtggtca gaaaggccga gccctttttc
      361 gaagccctcg gcgtcggcgg catggggaca cgcggaatgg gacggctcaa ggtgctctca
      421 tgctgcgacg cgagtgcgtc agcggcagcc ttagcgacag gcggccttcc aaatggggag
      481 gcgagccgat gagcgacggt aacaagacga ttcgggacat tgacctcgcc tgcgcggaag
      541 cgggccgcag gctcgccgcg ctcggcaata gggcaaagga cgccgagaac acgctgaaca
      601 aggccctcgg cgtgctggaa gagcagggtg tgtatgcaat gtttttgtat ctcttcgccc
      661 gggaggagcg ctgtggcgta gacgagctgt gccagcttct cggagacacg tgtgacaccg
      721 acgtcccgag acagtgcggt gcggaactga aggatctgga gagccggctc cagaacgcgc
      781 gaagacagga gaaggagcag gggcggcaac agaagcccca gcagcgaggt cggtcgggtc
      841 gtcaccagac ggaagcaggt gtgtcggaaa tcacgcgact cgaagaggag attcagaaaa
      901 agaagaccga gttcttcaac aaagtcgagt cgttggccaa cgacctcgat cagcttcttt
      961 tcgcccgcga ggtcctccac aacgcgctcg tgtacgctcg ctaccacgcc aaggcgcggg
     1021 caagtgcagg agagagggaa tgacctggtc gctctaccgc tggacctggc agcttgaatc
     1081 gccgctctac gtcgggatgc cgccggctgg aagcctcaac cggtgccggc tctacgtgcc
     1141 cgcgcgggcg ctgtggggcg cggtgacagc cgagctggcg cgcgcaaagg gcgcaagcac
     1201 gcctgctgat tacaagcggg tcggcgaaca ggtatctgag cacgtgcgtt tcacgtacct
     1261 gtatccggcc gaagcgaccg ggaaggactg gagggcgtgg cttccgaagt acgaaagagg
     1321 agaaggattg gtctgggaac gtgaggatcg ggttggcgat ccccgctcgg accgagcttt
     1381 tcgaacccgc cttttgagtt cccggcccgg caccgcaatc gacccgggta cggagacggc
     1441 cgccgatggg tcgctccgcg agacggaatg catcaacgag cagtggcgag atacggacgg
     1501 tcgagcggga tcgcgggtgg cgctggtcgg ctacctgttc atatgcaccg cgaacggcgc
     1561 agcggctagc cctatcgccg cgagcgagct cgaaggtctc gacacgctgt tcatcggcgg
     1621 cgacacgcga tacgggctgg gcaagctgag cagggcattc ccaggaggat gggaggaggc
     1681 cacttcggtg ttcggggcgg cggtcgatgt tgcggggccg caccctcgaa tcaggagcgg
     1741 ccgggtcctg gcccatgcga gaccccccag aacgggggga agagaggttt tcggacaatt
     1801 ggaactggtg gcaggttggg acgaccggaa cctggttccc caggacaccg gaccgcttct
     1861 cgcccctggc tcgagctccg gcgaggagct gtggtggaga ctggagccga gcggcttttg
     1921 gaatccggcg agcatcgcgt gaacattttc gagcttcacc gcaacgtcct caccgactac
     1981 cgcgacttcg tccgctcgtt cttcctgatc gcggacgagc gagcacggga gttcgtggat
     2041 cgggcgatcg agcaagagca gcgcctctgg cccgagccgc tcgtccaact gagcccggcg
     2101 tatgtgcccg gcccgaacgt cgacgaactg gcgcgcgatg gtctcgtagc gcaagagaca
     2161 gcgcgcatct tttcctggcc ggacggtaca acgttccgtc tctaccgcca ccaggaagaa
     2221 gcgatccgca aggtgcttgc cggggaaagc atcgtcgtga ccagcggaac gggctccggc
     2281 aagagcctgt gctacttcct gccgatcgtg gacagcctga tccgaaggcc cgagaccggg
     2341 gagcgagtcg ccgcactgat cgtgtatccg atgaacgccc tgatcaactc ccaattccaa
     2401 gccttgagcg gcttgaagga gcgctacgaa cgagcgctgg ggcggccgtt tccggtgacg
     2461 ttcgccaaat acacgggaga cacgccggac tcggatcgcg acgacatgcg tcgcaaaccg
     2521 ccgcggatct tgctcaccaa ctacgtgatg gccgagctgc tgctggtccg tccggaagat
     2581 cagcggtttc tcgacagagc tggaggcggc ctgcgctttc tcgtcttcga tgagctacac
     2641 gcgtaccgcg ggcgccaggg agccgacgtg gcgatgctcg tgcgccggct caaagaacgc
     2701 tgcgccgagg agggcctcgt ccacatcggc acgagcgcca cgatgatcgc ccgccctgag
     2761 gccacgagag aggaacggtg ccggagcgtc gccgacttcg ccgcccggtt tttcggccat
     2821 ccgtttagcc gcgagcaggt gatcgaggaa actttggtgc ccttcaccca gggcggcccg
     2881 ccttcggctg gagagttgcg atc
//
LOCUS       DATMBB010000257         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_290518, whole genome shotgun sequence.
ACCESSION   DATMBB010000257 DATMBB010000000
VERSION     DATMBB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290518"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VNL92_05525"
     CDS             <1..1077
                     /locus_tag="VNL92_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94205.1"
                     /translation="APPGLSWAPPDLVMGTGRNVVVRDAVHPFAEYIDEFRKRVAYRA
                     HFREGAAFGEGAKIFARSHGGAAVGVDIPLGAGRIVFIPPLPDLKPGTERSPVASCIL
                     DAGRKLLEMPPDEEAPEWTRSYALPGLEQKEAALAEAEEALVAAEARFEAAYRDEEDL
                     ARYRGLLWREGPWALDPIVHQAFRLLGFEVNGPPEGPAVLKADGVEIMLEVEGAPGPV
                     DMAPHYRLRDRREQWLAERGALPRGLVVVNGERLAPPERRQHPYIDALRIASESMGYA
                     LLTATDLFGLVQHALADTGAAWRASVRRQIAEAAGPLRFDDPRAEAEVEPPVSEAAEA
                     EPESAPEVEPTSRPDAAPARPRAH"
     gene            1098..1898
                     /locus_tag="VNL92_05530"
     CDS             1098..1898
                     /locus_tag="VNL92_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO94206.1"
                     /translation="MTQTDGRDWALILGASSGFGAAAAIELARSGFDIFGVHLDMRAT
                     IENARRVQLDIEGLGRRAVFFNQNAASPQKRAIVIDAIADELARTGTTVRVLLHSLAF
                     GSLMPYFGSNAEERVSQAQMEMTLDVMANSIVYWTQDLVERGLMTSGGRIFGMTSAGS
                     LRVFESYGEVSAAKAALESHIRQIAFELAPKGIRANAIRAGVTDTPALRKIPGAQRLY
                     NIAVERNPSGRPTTPEDVARAIALLCDPRADWITGNVINVDGGEEITV"
     gene            1901..2269
                     /locus_tag="VNL92_05535"
     CDS             1901..2269
                     /locus_tag="VNL92_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94207.1"
                     /translation="MGSDMRDVPRIRLVFYALFALLTALAVARLVDPDSTTPQPSISE
                     VRTNPADWLDHEVQLTGTLLETPGTVRYVLDDDGNRIGITGVSEEALRDLTGSDVEAR
                     GRVDYQRDRGITLNVESFSA"
     gene            2274..>2590
                     /locus_tag="VNL92_05540"
     CDS             2274..>2590
                     /locus_tag="VNL92_05540"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWO94208.1"
                     /translation="MPNYVSGSVSLHYEERPAAGVPERTLVFVHGWTGRGAGWRTLMD
                     SLSESYDSFAPDLRGCGESDHPPGGYTIEQYASDVFTLCELKGIANVDLVGHSMGGAV
                     ALTL"
BASE COUNT          426 a          851 c          880 g          433 t
ORIGIN      
        1 gcgccgccgg gcctctcgtg ggcgccgccc gatctcgtga tgggcacggg ccggaacgtc
       61 gtcgttcgcg acgcggtgca tcccttcgcg gagtacatcg acgagttccg gaagcgtgtg
      121 gcctatcggg ctcactttcg cgagggcgct gcattcggcg agggagccaa gatcttcgcg
      181 cggagtcacg gcggagccgc cgtgggggtc gatattcccc tcggcgccgg gcgcatcgtc
      241 ttcattccgc cgcttcccga tctcaaaccc ggcacggagc gatcgccggt ggcgtcgtgc
      301 atcctcgatg cggggcggaa gctcctcgag atgccgccgg acgaggaagc gcccgagtgg
      361 acgcggtcct acgccttgcc gggcctcgag caaaaggagg cggccttggc cgaggcggag
      421 gaggcgctgg tcgcggccga ggcgcgattc gaggcggcct atcgggacga ggaggatctg
      481 gcacgatatc ggggcctcct ttggcgcgaa ggaccgtggg cactggatcc gatcgtccat
      541 caggcgttca ggctcctcgg cttcgaagtg aacggccctc cagagggccc tgctgtgctg
      601 aaggccgacg gcgtcgagat catgctcgag gtcgagggtg ctccaggtcc ggtcgacatg
      661 gcgccacact atcgattgcg agatcggcga gaacagtggc tggcagagcg cggggcgctt
      721 ccgcgaggcc tcgtggtcgt gaacggcgaa cggctcgctc ctcccgagcg ccgccagcat
      781 ccgtacatcg acgcgcttcg tatcgccagc gagagcatgg gatatgctct gctcacggcc
      841 accgacctgt tcggccttgt tcaacacgct ctcgccgaca cgggagcggc gtggcgcgcc
      901 tcggtgcgac ggcagattgc cgaggccgcg gggccgttgc ggttcgatga tcctcgtgct
      961 gaggcagaag tcgagcctcc ggtatcggaa gcagcagagg cggagccgga gagcgcgcca
     1021 gaggttgagc ccacgagccg gccggacgct gctccggcgc gaccgcgagc gcactaagaa
     1081 tagggaggcg cacatccatg acgcagaccg acgggcgcga ctgggccctc atcctgggcg
     1141 catcgagcgg attcggagcg gcggcggcca tcgagctggc acggagcggc ttcgatatct
     1201 tcggcgtcca tctggacatg cgcgcgacaa tcgagaatgc gcggcgtgtc cagctcgata
     1261 tcgagggcct tgggcggcgc gcggtattct tcaaccagaa cgctgcgagt ccccagaagc
     1321 gcgccatcgt gatcgacgcc atcgccgatg agttggctcg cacaggcacg actgtccgcg
     1381 tgctccttca ttcgctcgct ttcggctccc tcatgccgta ctttggctcg aacgccgagg
     1441 agcgcgtctc gcaggctcag atggagatga cgctcgatgt catggcgaac agcatcgtct
     1501 actggacaca ggacttggtg gagcggggcc tcatgacctc gggcggccgc attttcggga
     1561 tgacgagtgc cgggtcgctg cgcgtgttcg agtcctacgg agaggtgtcc gccgccaagg
     1621 ccgcgctgga gtcgcacatc cgccaaatcg cgttcgagct cgcgccgaag ggcatccgcg
     1681 ccaacgcgat tcgtgccggc gtgaccgata ccccagcgct ccgcaagatt ccgggggcgc
     1741 agcgcctcta caatatcgcc gtcgaacgca accccagcgg gcgcccgacc acgccggagg
     1801 acgttgcgcg cgctatcgct ctcctctgcg accctcgcgc cgactggatc acgggcaacg
     1861 tcatcaacgt cgatggcggc gaggagatta cggtgtaacc atgggaagcg acatgcgcga
     1921 tgtccctcgc atccgtctcg tgttctatgc cctgttcgcg cttctgaccg cgctcgccgt
     1981 cgctcgtctc gtcgaccccg actcgacgac gccacagcca tcgatcagcg aggtgcgaac
     2041 gaacccggcg gactggctcg atcacgaagt gcaactcacc ggaaccctcc tggagacgcc
     2101 cggcaccgtt cgttatgtcc tggatgacga tgggaaccgc atcggcatca ccggcgtatc
     2161 cgaggaagcg ctgcgcgacc tcaccggctc cgacgtcgaa gcgcgtgggc gcgtggacta
     2221 tcaacgcgac cgcggtatca ccctcaacgt cgagagcttc agcgcctagt tccgtgccga
     2281 actacgtttc gggcagcgta tcgctgcact acgaagagcg gcctgccgcg ggcgttcctg
     2341 agcgcaccct tgtcttcgtc cacggctgga cggggcgggg cgccggctgg cggacgctca
     2401 tggacagcct gtccgagagc tacgactcct ttgcccccga cctccgtggc tgcggagaga
     2461 gcgatcaccc gcccggcggc tacacgatcg agcagtacgc cagcgacgtc ttcaccctgt
     2521 gcgagctcaa ggggatcgcg aacgtcgatt tggtgggaca ttcgatgggg ggagccgttg
     2581 cgctcaccct
//
LOCUS       DATMBB010000258         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_301339, whole genome shotgun sequence.
ACCESSION   DATMBB010000258 DATMBB010000000
VERSION     DATMBB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301339"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VNL92_05545"
     CDS             <1..540
                     /locus_tag="VNL92_05545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWO94209.1"
                     /translation="GTQLLEENGEIQLSARAFPGYSTALFNRHSMLTRLLPGNRFSRR
                     YLLSSWAHDETRDVDWVSGACMVLARRGIESFGAFDPAYFMYIEDVDLCLRAKKAGFR
                     VVYFPAVRVRHTIGGSSRSATFRMIRARHASMARYYRKHLRRGFVFDSLTVGGIWARC
                     GLQFALAAVRRVAGRPHRP"
     gene            complement(556..1719)
                     /locus_tag="VNL92_05550"
     CDS             complement(556..1719)
                     /locus_tag="VNL92_05550"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWO94210.1"
                     /translation="MTVELLSLSVAFVAAVALTTLLRVRTAATPRGIISFPTRRVDDM
                     PRIGGIAIFLAFLITPIIVASVSNDASERLSDDWREVAGFGASGGLVLLLGIFDDIRI
                     AHFRQKLAVQIVAALLLYVSGFRIEELAFPGNLSVDLGWSAPFMTIVWVVGVSNAVNL
                     IDGKDGVATGVSAIVAGTLMLTAWNMDNALAAMLFATLTGSVLGFLPFNFPPASRYLG
                     DSGALFLGFAIAALSIKGSVNIAGHVFLGIPLVALGFPIVDTVLAATRRLADGRHPML
                     GDEDHIHHRLASTAGLGPRSLALATYGLTGLFAAAALILHFVPDAVGLPLVIGLVGMT
                     IAALLTWLGYPQSFWDSAVSRAVRRVPPREAMPPSVLTRHPRRQSEPRADESG"
     gene            complement(1873..2310)
                     /locus_tag="VNL92_05555"
     CDS             complement(1873..2310)
                     /locus_tag="VNL92_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94211.1"
                     /translation="MAALTFPSVDWFDAIRNMMNNDPEFRKLGTCDAEVGIEVRDTGQ
                     RFVLTFEAFECTGVREATQAELDATDFYLSMDLADWKEMIQNIKQNGGADLDHTLNTL
                     DMAREDSLAHSKDEYRRDLFYRFNQTFQDFFNASAKLDTNFPN"
     gene            complement(2366..>2969)
                     /locus_tag="VNL92_05560"
     CDS             complement(2366..>2969)
                     /locus_tag="VNL92_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94212.1"
                     /translation="EVKAFLSTQIWDASHQFEVFRKRALSNGGGLGVQSPGLFARSIV
                     NARDWTEVTVFLHLLRDSFLQTLFAWGERFAHNPAEKAIFAYSLQDVTRHITYGVDHI
                     RFFLHKRPERHREIINYVTLGETAAAAEWEKDVPQNEALAIILGGGKDNIGEGFRLLK
                     QLRRDQIENYMRHARAARIGDRRDSLAKGLAAYFTEAVTA"
BASE COUNT          547 a          879 c          992 g          551 t
ORIGIN      
        1 ggaacgcagc tcctcgagga gaacggcgag atccaactct cggcccgggc cttccccggc
       61 tattcgaccg cgctattcaa ccggcactcg atgcttacgc gattgctgcc cgggaacaga
      121 ttctcccgtc gctacctact atcgagctgg gcgcatgacg agacgcggga cgtagattgg
      181 gtatcggggg cgtgcatggt gctggcgcga cgaggcatcg aatcctttgg cgcgttcgac
      241 cctgcgtact tcatgtacat cgaggacgtc gacctgtgtc tgagggcaaa gaaggcaggc
      301 ttccgcgtcg tctactttcc cgccgtgcgg gttcggcaca cgatcggggg cagctcgcgc
      361 tcggcgacct tccgcatgat ccgcgcccgc cacgcaagca tggcgcgcta ttaccgcaag
      421 catctgcgtc ggggcttcgt attcgacagt ctgacggttg gcgggatatg ggcgcggtgc
      481 gggctgcagt ttgctctggc cgctgtgcga agggtcgcgg gtcgtccgca ccggccctag
      541 tgctccgcgc gcctcctatc cactctcgtc tgcgcgtggc tcgctttgcc tccgggggtg
      601 ccgcgttagc acggagggag gcatcgcctc tctcggaggc actctgcgta cggcgcgact
      661 caccgcgctg tcccagaacg actgcgggta cccaagccag gtgagcagcg cagcgatcgt
      721 catgcctacg aggccgatca cgagtggaag gccgacggca tcgggcacga agtgaaggat
      781 gagcgcggcc gccgcgaaaa ggccggtgag accgtaggtt gcgagcgcga ggcttcgcgg
      841 gccgaggccg gctgtcgacg cgagccggtg gtggatgtgg tcctcgtcgc ccagcatggg
      901 atggcgaccg tccgcgagcc ttcgcgtcgc ggccaggacc gtatcgacga tcgggaaccc
      961 gagcgccacg agcgggatgc cgaggaagac gtgaccagcg atgttcacgg agcccttgat
     1021 cgacagcgcc gcgatggcaa agcccaggaa gagcgcgcct gaatcgccga gataccgcga
     1081 ggccggaggg aagttgaacg gcaggaaacc gagcacactg ccggtcagcg tggcgaagag
     1141 catggcggcg agcgcattat ccatgttcca tgccgtgagc atgagagtac cggcgacaat
     1201 cgcgctcacg cccgtggcaa cgccatcctt gccatcgatg aggttgacgg cgttgctgac
     1261 gcccacgacc cagacgatgg tcatgaacgg cgccgaccag ccgagatcga ccgagaggtt
     1321 gccgggaaac gccagttcct cgatgcggaa gcccgagacg tagagcaaca atgcggccac
     1381 gatctggacc gccagcttct gtcggaagtg ggcgatacga atgtcgtcga agatgccgag
     1441 cagtagcacg aggccgcccg aggcgccgaa gccagcgacc tcacgccagt cgtcggatag
     1501 ccgctcggag gcgtcattgg acacggaggc gacgatgatg ggcgtgatga ggaaggcgag
     1561 gaagatggcg atgccaccaa tgcggggcat gtcatccact cgccgtgtgg ggaagctgat
     1621 gattccccgc ggtgttgccg cggtgcggac gcggagcagg gtggtgaggg cgacggccgc
     1681 gacgaaggcc acggagaggg acaggagttc gaccgtcaag ctaacgtttc ctcatgcttg
     1741 ggggctaaac ggcccgccag agcggcattg tagcacggcg ggccctaacg atcgccgggg
     1801 ccaagcgaaa cgaagatgag ggatcggtct ttgccgatcc ctcatcgatc ccctagccga
     1861 tgttctgcgt gactagttcg ggaagttcgt gtccaacttc gccgacgcgt tgaagaagtc
     1921 ctggaaagtc tgattgaagc ggtagaacaa gtcacgccgg tactcgtcct tggagtgcgc
     1981 caagctatct tcgcgggcca tgtcgagcgt gttgagcgta tggtcgaggt cggccccgcc
     2041 gttctgtttg atgttctgga tcatctcctt ccagtcggcg aggtccatcg aaaggtagaa
     2101 gtcggtggca tcgagctccg cctgggtcgc ttctcggaca ccggtgcact cgaacgcctc
     2161 gaaagtgagg acgaatcgct ggcccgtgtc acgaacctcg atgccgacct cggcatcgca
     2221 cgtgccgagc ttcctaaact cagggtcgtt gttcatcatg ttgcggatgg cgtcgaacca
     2281 gtctacggat ggaaacgtga gagcagccat tgctttcccc tctgctggtt tggaatgctc
     2341 gcgtcggtcg cggtcctatc gggtgttatg cggtcacggc ctcggtaaag taggcggcca
     2401 agcccttggc taggctatca cgccgatcgc cgatgcgtgc cgcgcgtgca tgacgcatat
     2461 agttctcgat ctggtctcgt cgtagttgct tgagtagccg gaagccttcc ccaatgttgt
     2521 ccttgccacc tccgaggata attgcgaggg cctcgttctg cggtacgtcc ttctcccact
     2581 ccgctgccgc ggctgtctcg ccgagtgtga catagttgat gatctcgcga tgacgctcgg
     2641 gtcgcttgtg gaggaagaat cgaatgtggt cgactccgta ggtgatgtgt ctcgtcacat
     2701 cctgcagact atacgcgaag atcgccttct cggcagggtt atgggcgaac cgctcgcccc
     2761 aggcgaagag cgtctggagg aagctgtctc gcagcagatg caggaagacg gtgacctccg
     2821 tccaatcgcg ggcgttgacg atcgagcgcg caaagaggcc cggactctga actcccaggc
     2881 cgcccccgtt tgagagcgct cgctttcgaa agacctcgaa ctggtggctg gcgtcccaga
     2941 tttgggtcga gaggaacgcc ttcacttcg
//
LOCUS       DATMBB010000259         2092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_129836, whole genome shotgun sequence.
ACCESSION   DATMBB010000259 DATMBB010000000
VERSION     DATMBB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129836"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VNL92_05565"
     CDS             <1..870
                     /locus_tag="VNL92_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94213.1"
                     /translation="TPLVDLPPKPGHEVKEAIAEAADGRVIVKRRTGLVIVVRPGEYF
                     VLGDVTKHEHQVHNVIGNAFASGFLEQGYVMLHASAVSDPSVGMAFGARSGGGKSSLA
                     LAMLERGFRFVTNDRLFVRPIDDGVQMLGMPKMPRVNPGTLVRLPSLRSLLDREERRL
                     IATLSHRELWELERKHDVDVDSLYGPGTWVTDVVPLDGLYILHWSLDGDGLEVRTVES
                     VAERFRELRGLLKGLGPYAPRRAHHLSPAGLDAIAARLPVYRVEGRADVVLLSERLAA
                     GVRSPAGSIDGQS"
     gene            881..1999
                     /locus_tag="VNL92_05570"
     CDS             881..1999
                     /locus_tag="VNL92_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWO94214.1"
                     /translation="MTIGAANAERLLVLDPASFGLGRKCTVTPRPLGAGGSGLNVAAR
                     LLATGHAVFPILPVAPDGTGQALLQVLHDAAERGGQTVNVESFMMDGDTSTTPLTTIL
                     VLGDQRTVLSEFPDAIVREFVVHWRRRLARLGPVMPSAVVIGHIHADRPTSEQGQSGG
                     ISQAVIEMFAERGIPIFVNFGASQYTLGIARWSHLLDRVACLQLDIDEARRFVHGEVR
                     PTLHAMLDWFRDRCTVVITLERLGAVAQFRGSDSVIFAWPYDLPPDVIADTTGAGDAF
                     VAGVVSSALDVPLEDEDALLKAVEKGRLWASYACTTFGGANDCPTHEQLAQFPISHSL
                     LLQSERRRMSEAVPLLRILDRSFASTAPARSPSARPAG"
BASE COUNT          321 a          694 c          693 g          384 t
ORIGIN      
        1 acccctctcg tcgacctgcc gcccaagccc gggcacgagg tgaaggaggc aatcgccgaa
       61 gcggccgatg gtcgcgtcat cgtgaagcgt cgtaccggac tcgtgatcgt ggtgcgaccg
      121 ggagagtact tcgtcctcgg cgacgtgacg aagcacgagc atcaggtcca caacgtcatc
      181 ggaaacgcct ttgcctccgg gttcctcgag cagggctacg tgatgcttca tgcctcggcc
      241 gtttcggatc cgtcggtcgg aatggcgttc ggtgcacgat cgggcggggg caagagcagc
      301 ctcgcgctcg ccatgctgga acgcggcttc cggttcgtga ccaacgaccg gctcttcgtc
      361 cgtccgatcg atgatggtgt ccagatgctc ggcatgccca agatgccgcg cgtcaacccg
      421 ggcactctcg ttcgcctgcc gtcgctacgt tcgctcctgg accgggagga acggcggctc
      481 attgcgaccc tctcccaccg ggagctctgg gagttggagc gcaagcacga cgtcgatgtc
      541 gatagcctgt acggaccggg aacctgggtt accgatgttg tccccctcga tggcctctac
      601 attcttcact ggtcgctcga cggcgacggc ctcgaagtac ggaccgtcga gagtgtggcc
      661 gagcgctttc gagaactgcg cggcctgctc aagggcctgg gtccctacgc gccgcgccgc
      721 gcgcatcatc tatcgcccgc cgggctcgac gccatcgcag cgaggcttcc ggtctatcgc
      781 gtcgagggtc gggccgacgt cgtattgcta tcggagcgcc tcgcggcagg agtacgatcg
      841 cccgccgggt ccatcgatgg ccaatcatga gcgtccgatc gtgacgatcg gagcggcgaa
      901 tgcggaacgt ctgttggtgc tggaccccgc atcgtttggc ctggggcgca aatgcacggt
      961 gactccgcgg ccgctggggg ccgggggcag cggcctgaac gtcgcggcgc ggctgctggc
     1021 cacgggtcat gccgtgttcc cgattctgcc cgtggcgccg gacggcaccg gccaggccct
     1081 cctgcaggtc ctccacgacg ccgccgagcg tggcggtcag accgtcaacg tcgagagttt
     1141 catgatggat ggtgacacct cgacgacgcc cttgaccacg atcctcgtgt tgggcgacca
     1201 gaggacggtg ctgagcgagt tccccgacgc tatcgtgcgg gagttcgtcg tgcactggcg
     1261 acgacggctc gcccgtctcg gtcccgtcat gccctcggcg gtcgttatcg gccacatcca
     1321 tgccgaccgg ccgacctccg agcaggggca gagcggaggc atttcgcagg ccgtcatcga
     1381 gatgttcgcg gagcgcggca tccccatatt cgtcaacttc ggcgcctctc agtacaccct
     1441 cggcatcgcg aggtggagcc acctccttga tcgcgtcgcg tgcctgcaat tggatatcga
     1501 cgaggcgcga cgattcgtcc acggcgaggt gcgaccgacc ctccacgcca tgctcgactg
     1561 gttccgcgat cggtgcacgg tggtcatcac gctcgaacgc ctgggcgcag tggctcagtt
     1621 tcgcggaagc gatagcgtca tctttgcgtg gccttacgac ctgccgccgg atgtgatcgc
     1681 ggataccact ggcgcgggcg acgcattcgt ggccggcgtc gtttcgagcg ctctggacgt
     1741 tccactcgaa gacgaggatg cgctcctgaa ggccgtggag aagggccgcc tttgggcgag
     1801 ctacgcctgc acgacgttcg gcggcgccaa cgactgtccg actcacgagc agctggcgca
     1861 gtttcccatc agccactcgc ttctgctgca aagcgagcgc aggcgcatgt cggaggccgt
     1921 tccgcttctg cgtatcctcg accgaagctt cgcatcgacg gcgccggcac ggagcccgtc
     1981 cgcccgtccg gcggggtgat ggcgtcgtcg ccatggccgc gtctgggcgc atctcgagag
     2041 tcacgtcgca cgacgggaag acgttgagcg aaccgccctc attgctcttc at
//
LOCUS       DATMBB010000260         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_40733, whole genome shotgun sequence.
ACCESSION   DATMBB010000260 DATMBB010000000
VERSION     DATMBB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40733"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..507)
                     /locus_tag="VNL92_05575"
     CDS             complement(286..507)
                     /locus_tag="VNL92_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6117 family protein"
                     /protein_id="HWO94215.1"
                     /translation="MSIPDHARMNFATLLRAAAGGNLALMECTDAVTGEPRYVICAVG
                     RDGTDFVFTPFGHLADGNPFDAYVPPSTP"
     gene            complement(504..929)
                     /locus_tag="VNL92_05580"
                     /pseudo
     CDS             complement(504..929)
                     /locus_tag="VNL92_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599811.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1002..1196)
                     /locus_tag="VNL92_05585"
     CDS             complement(1002..1196)
                     /locus_tag="VNL92_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94216.1"
                     /translation="MYGTYLRLDITVWTRNRAVIRAARRKLTRAAQRDPAKREARKRF
                     YRQMLADHANAQRHVAEFRL"
     gene            complement(1198..1851)
                     /locus_tag="VNL92_05590"
     CDS             complement(1198..1851)
                     /locus_tag="VNL92_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94217.1"
                     /translation="MPAYTVETTYRVPVYRHRTYHAATPAEACRQAIEDDDWSDEKLD
                     YEAAGETYVTGIWLGADAAYSGETVPVPSHFDETVQRKAAHFEVLFGLLKMVVADQVA
                     QRRTDAYWLARGNAAVAKAEAILAGASDPDDPVTAPRPRHVLAQLQEDRVCDQIAAIV
                     EADPEFAGLTADAVPDADIHSACVSVAASIDLSEEIGAAEFRAALAALRAAKQAKGG"
     gene            complement(1924..>2115)
                     /locus_tag="VNL92_05595"
     CDS             complement(1924..>2115)
                     /locus_tag="VNL92_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94218.1"
                     /translation="RVVFQYLVPVHVEVEDGLVHSVKVIDETTVESPTVVEGDADYLE
                     EAVEAANDGQPWPSWQFGF"
BASE COUNT          344 a          654 c          764 g          353 t
ORIGIN      
        1 aagggttgcc gcgtcgacct gacggccctg ttcgagatgc ggaaggagat ggtgcgcggc
       61 tgcgccgatg cagcgagcga tatcggaggg agacgcaagc ggcgacgccg cgaccgcagg
      121 cgcggtcgtg agaccgttgg atggggtcat gtcggtgaac ctcggagagc ggggacaggc
      181 gagccggccg gcgctctctc tggaccgcac cggctcaaac ccgtcccggc caggctctcc
      241 ctctcgtcac gcagccttca ccgcgcgccg ccacgtacgc gaggatcagg gtgtcgaagg
      301 aggaacgtag gcgtcgaagg gattgccgtc ggcgagatgg ccgaagggcg tgaacacgaa
      361 atccgtgccg tcgcggccca cggcgcagat gacgtaacgc ggctcgccgg tgacggcgtc
      421 ggtgcactcc atgagcgcga ggttgccgcc cgccgccgcg cgcagcagcg tcgcgaaatt
      481 catccgtgca tgatcgggga tgctcatggc gcacctcgct ttcccggccg agcggacaga
      541 gtgcggtcga gccagggatt ggtcgcgatc cacgcgacgg tcttgcggcg gccgagatcg
      601 accacatgcg cgccgccccc gaacgcgttc acacgcgggc gcgagcaggt gttcgcccat
      661 tggaaaccct agcgtccggt gaggccgaat tcctccgcgc aacggagcac gaagtcgatg
      721 acggcctgcg gatcgccggt cgtgtcatcg cgaatccaga gctgcgttcc accatgctcg
      781 ggctggatgg agaggaggaa cgcctcggac ggcgggtcct cggcggcgtt ctcctccatc
      841 aggcgattgt agatttcgag agcgcgtgcg gcgttctccg gcgtgcggac atcgagaaga
      901 caggagaagt gcgtgaagaa gtcagccatg tcaggctcct gaaacgacga agcccggcgc
      961 gatggccggg ctggtttagg gacggggttg ttgctagagc gtcagagccg gaattcggcg
     1021 acgtgccgct gcgcgttggc gtgatccgcg agcatctggc ggtagaagcg cttgcgcgcc
     1081 tcccgcttcg ccggatcacg ttgcgccgct cgcgtgagct tgcgccgggc agcgcggatc
     1141 actgcacggt ttctggtcca gaccgtgatg tcgaggcgga gataggtgcc gtacatctca
     1201 gcctcccttc gcctgctttg ctgcccgcag ggcggcgagc gccgcgcgga actcggccgc
     1261 gccgatttcc tccgagagat cgatgcttgc ggcgacggac acgcatgcac tgtgaatgtc
     1321 agcgtccggc acggcgtccg cggtcagacc tgcgaattca ggatcggcct cgacgatcgc
     1381 ggcgatctgg tcgcagacgc ggtcctcctg gagctgtgcg aggacgtgac gtgggcgtgg
     1441 agccgtgacg gggtcgtcgg gatcgctcgc tccagcgagg atcgcttcgg ccttggcgac
     1501 cgcggcattc cctcgcgcga gccaataggc atccgtccgc cgttgtgcga cctggtccgc
     1561 aacgaccatc ttgaggagac cgaacaggac ctcgaagtgc gcggccttgc gctggacggt
     1621 ctcatcgaag tgcgatggga ctggaacggt ctcacccgaa taggcggcat cggccccgag
     1681 ccagatgccg gtgacgtagg tttcgccggc ggcctcgtag tcgagcttct cgtcggacca
     1741 gtcgtcgtcc tcgatggctt ggcggcacgc ttctgccggc gtcgcggcgt gataggtgcg
     1801 gtgccggtag accggcacgc ggtaggtcgt ttcgacggtg taagcgggca tgacaggctc
     1861 ctgaaaacgc gaaagcccgg cgcggcggcc gggctgttgg ggttgggtgg agggaaggcg
     1921 ggcctagaag ccgaattgcc aggacggcca gggctggccg tcattggcgg cctccaccgc
     1981 ctcctcgaga tagtcggcat cgccttccac gacggtcggg ctctcgacgg tcgtttcgtc
     2041 gatcaccttc acgctgtgga cgaggccgtc ctcgacttcg acatggacgg gcacgagata
     2101 ctggaagacg acgcg
//
LOCUS       DATMBB010000261         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_196482, whole genome shotgun sequence.
ACCESSION   DATMBB010000261 DATMBB010000000
VERSION     DATMBB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196482"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /gene="gcvPB"
                     /locus_tag="VNL92_05600"
     CDS             <1..1316
                     /gene="gcvPB"
                     /locus_tag="VNL92_05600"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWO94219.1"
                     /translation="VRYFTALSSLNYGVDSGFYPLGSCTMKYNPKANEAIASLPGFAD
                     IHPLQPEESVQGVLAALHDLQRCIAALTGMDAVCLTPFAGAQGELAGILCVKAHHEAN
                     GEGGRRRTVLVPDSAHGTNPATAAMCGYEVRAVPSDRAGNMDLGRLEEMADDSVAALM
                     LTMPTTLGLFDPNIERIASLLHDRGIQLYGDGANMNALIGRARFGDLGFDLVHLNLHK
                     TFSTPHGGGGPGAGPVAARAHLAPFLPTPIVEKRESTFHLAAPERSIGRLGAFQGNVG
                     VLLRAYAYIRALGLEGLRSVSENAVINANYVRTRLAGRYQLPYDRPCMHEVVFSGSRQ
                     KAQSGVRTLDIAKRLIDYGFHPPTIYFPLIVDEALMIEPTESEALEGLDAFCEAMLAI
                     ADEAESNPEVLRDAPSTAPLRRLDEAAAARRPVLRWRPDPRTADE"
     gene            1334..2419
                     /locus_tag="VNL92_05605"
     CDS             1334..2419
                     /locus_tag="VNL92_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWO94220.1"
                     /translation="MLPTISAGTLFLACCASGSGDATIAPQNDIAAPRADNLHLPDGF
                     VAYRYAQGFEEPTAIELAPDGMLWLSERGGRIIALPDTNDDLVAEPRTFAENFDLQEL
                     LGIAVDEDDVVYASSRGRISVITDDDGDGDADSIDDIVTDLPNGRHQNNNIVLGRDER
                     LYFGNGSTCNLCDDESEMSGAILSVKPNGDDLRVFATGLRNPYGIIFSADGNLYVTDN
                     GPDPPDIQGAPDELNRIVEGDDYGWPDCIGGQELEAGACEESTPPLALLQQRSSSDGL
                     ATYDAEDFPEEYRHAAFIAQWGPTSEDIDAGRRVVYVQVREEVPQERVFATGFDRPID
                     VAADLEGRLYVADFATGIVYVIVYEGA"
BASE COUNT          423 a          848 c          791 g          402 t
ORIGIN      
        1 tggttcgata ctttaccgcc ctctcgagcc tgaactacgg cgttgacagc ggcttctacc
       61 cattgggcag ctgcacgatg aagtacaacc caaaggcgaa cgaagcgatc gcctccctgc
      121 cgggcttcgc cgacatccat ccgctgcaac cggaggaatc ggtgcaaggc gtgctggcag
      181 cgctgcacga tctccagcgc tgcatcgccg cgctcaccgg catggacgcc gtgtgcctca
      241 ctcccttcgc cggagcccag ggcgagctcg ccggcatcct ctgcgtgaag gcccaccacg
      301 aggcgaacgg cgaaggcggg agacggcgca cggtcctggt gcccgattcc gcccatggta
      361 cgaacccggc gaccgcggcg atgtgcggct acgaggttcg cgcggtgcca tccgaccgtg
      421 ccgggaacat ggacctcggg cgtctagagg agatggcgga cgatagcgtc gcggcgctca
      481 tgctgacgat gccaacgacg ctcggcctct tcgacccgaa catcgagcgc atcgcctcgc
      541 ttctgcacga ccgcggcatc cagctctatg gcgacggcgc caacatgaac gccctgattg
      601 ggcgagcgcg cttcggcgac cttggcttcg acttggtgca cctcaacctg cacaagacgt
      661 tcagcacccc tcacggtggc ggcgggccgg gtgccggtcc agtcgccgcc cgcgcgcatc
      721 tggcgccctt tctcccaaca ccgatcgtcg agaagcgcga gtccacgttc catctggccg
      781 cgccggagcg gagcatcggc cggctggggg cattccaggg caacgtcggc gtgctcctac
      841 gggcctacgc ctacattcgc gccctcggct tggagggctt gcgctccgtg agcgagaacg
      901 cggtcatcaa cgcgaactat gtacgaactc gcctggccgg ccgctaccag ctgccctacg
      961 accggccctg catgcacgag gtcgtcttct cgggatcgcg ccagaaggcc cagagcggcg
     1021 tgcgtacgct cgacatcgcc aagcggctca tcgattacgg cttccacccg ccgacgatct
     1081 attttccgct catcgtcgac gaggcgctca tgatcgagcc caccgaatcg gaagcgctcg
     1141 agggactcga cgccttctgc gaggccatgc tcgccatcgc cgatgaggcc gaatcgaatc
     1201 ccgaggtcct gcgtgacgcc ccgagcacgg cgccgctgcg gcgactcgac gaggcggcgg
     1261 ccgcccgccg gccggtgctg cgctggcgcc ccgaccctcg gacggcggac gaatgagagc
     1321 tcggcggctc ctcttgcttc ccaccatttc ggccgggacg ctcttcttgg cctgctgcgc
     1381 cagcggttcc ggcgacgcta ctatcgcgcc gcagaacgat atcgccgccc cgcgcgcgga
     1441 taatctgcat cttcccgatg ggttcgtcgc ataccgctac gctcagggat tcgaggagcc
     1501 gacggccatc gagctcgcgc cagacgggat gctgtggttg agcgagcgcg gcgggcgcat
     1561 catcgccttg cccgacacga atgacgatct cgtcgcggag ccacgcacgt ttgccgagaa
     1621 tttcgatctg caggagctgc tcggcatcgc cgtcgacgag gatgatgtcg tctacgcctc
     1681 gagccgcgga cgcatcagcg tcatcacgga tgacgacggc gatggcgacg cagacagcat
     1741 cgacgacatc gtgaccgatc tgcccaacgg ccggcaccag aacaacaaca tcgtcctggg
     1801 ccgcgacgag cgcttgtatt tcgggaacgg gtctacgtgc aacctctgtg acgacgagag
     1861 cgagatgtcg ggcgccatcc tctcggtcaa gcccaacggg gatgacctgc gcgtgttcgc
     1921 gaccggcctc cggaaccctt acggcatcat cttctcggcc gacgggaacc tctacgtcac
     1981 cgacaacggg cccgatccgc cggacatcca gggagccccc gacgagctga accgcatcgt
     2041 cgaaggcgat gactacggct ggcccgattg catcggcggg caggagttgg aggccggcgc
     2101 ctgcgaggag agcacgcctc cgctcgccct gctccagcag cgcagctcct ccgacggcct
     2161 ggccacctat gatgctgagg acttcccgga agagtaccgg cacgcggcgt tcatcgcgca
     2221 gtggggaccg acctcggagg acatcgatgc cggccgccgc gtggtctacg ttcaggtacg
     2281 ggaggaggtt ccccaggagc gcgttttcgc gaccgggttc gataggccca tcgatgtcgc
     2341 agccgacctc gaaggcaggc tctacgtcgc tgatttcgcg accggcatcg tatacgtcat
     2401 cgtgtacgag ggagcgtagc cgtgacgtgg cgcgcgacca gaggaatcgg agttgtggtc
     2461 ggag
//
LOCUS       DATMBB010000262         3092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_581481, whole genome shotgun sequence.
ACCESSION   DATMBB010000262 DATMBB010000000
VERSION     DATMBB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581481"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="VNL92_05610"
     CDS             <1..1086
                     /locus_tag="VNL92_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWO94221.1"
                     /translation="PGKSSMQLMAEACRNAMDDAGIDKSEVDAVLTCGFGFMPSVLVS
                     EYLGIVPKWTDGTSIGGSSFEVHVEHAAAAIKAGICEVALIAHGEAGKSGSRFRGGGM
                     DPTMPQLSFEMPYGIGAAPSVYSMACQRHMHQYGTTHEQLAEIAVATRKWAELNPKAL
                     MRDPIGIDDVLNSRWVAYPFHLLDCCLVTDAAGAVIVASPERAAKMKKKPVWVLGCGE
                     SNTHQSISQMPDLTQTPARHSGPRAFEMAQMKHADIDVVEIYDSFTYTALVTLEELGF
                     CKKGEGGRFVSGQRTAPGGDFPMNTNGGGLSYTHPGMYGIFILIEAARQLRGECGERQ
                     VPNAKTALVNGTGGVLSSTGTVILGVD"
     gene            1224..1871
                     /locus_tag="VNL92_05615"
     CDS             1224..1871
                     /locus_tag="VNL92_05615"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWO94222.1"
                     /translation="MLIDLHSHTWPLSDDSLLDPDDLIIRTKEAGIDGICLTEHDFFW
                     DHEKVRQLAHKHQFLVLPGCEVNTEQGHMLTFGLTEYRFGMHRVEELARMVDAVDGAM
                     IAAHPYRRQMPWQPNDPNDYHRALERAKQNRAYRYVAALEIENGRGSERENEFSRRLA
                     TELEMPGTAGTDSHARKDIARCATYFEADIRSLEDLIQALKAGRFYPVTLQRARA"
     gene            1904..2380
                     /locus_tag="VNL92_05620"
     CDS             1904..2380
                     /locus_tag="VNL92_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO94223.1"
                     /translation="MAEEQESVITDEMRAQIGKESEPVIYEVDKTTIRLYARAVGYDD
                     PLYYDEEFARSKGYRSLPAPPGFLGFPIYDPRNAGQQMGPPRFQTRLQRVLNGGSEFE
                     YFDDICAGDTLVATGRITDIYERPGRMGLMLFTEREQVFKRDGKVVAVSRGRSINY"
     gene            2438..2878
                     /locus_tag="VNL92_05625"
     CDS             2438..2878
                     /locus_tag="VNL92_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWO94224.1"
                     /translation="MATETRTQLYWDDVQEGQELPTLRKKPNSQQLVLWAAGSGDFYQ
                     IHYDKDFAKSTGLDNIIVHGLLKNAFQGQLLHDWIGYEGRIKKFGCSYRGMDPVDQEI
                     LVKGVVTKKYEAGGEYLVDVDVWTENPAGEKTTPGYGTVRLPRR"
     gene            2969..>3092
                     /locus_tag="VNL92_05630"
     CDS             2969..>3092
                     /locus_tag="VNL92_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO94225.1"
                     /translation="MGRLDGRVALITGSGRGIGRGMALLFASEGAKVVVNDPGVN"
BASE COUNT          618 a          968 c         1023 g          483 t
ORIGIN      
        1 cccggcaaga gcagcatgca gctgatggcc gaggcctgcc gcaacgccat ggacgatgcc
       61 ggcatcgaca agtcggaggt cgatgcggtc ctgacgtgcg gcttcggctt catgccgagc
      121 gtgctcgtat cggagtacct cggcatcgtg cccaagtgga ccgatggcac gtcgatcggc
      181 ggatcgtcgt tcgaggtgca cgtcgagcac gcggccgccg cgatcaaagc cggcatctgc
      241 gaggtcgccc tcatcgcgca cggcgaggcc ggcaaatcgg gcagccggtt ccgcggcggt
      301 ggcatggatc cgacgatgcc gcagctttcg ttcgagatgc cctatggaat cggggctgcc
      361 cccagcgtct actcgatggc gtgtcagcgg cacatgcacc agtacggcac gacgcacgag
      421 caactcgccg agatcgccgt ggccacgcgc aagtgggcgg agctgaaccc gaaggcgctc
      481 atgcgcgacc ccatcggtat cgacgacgtg ctcaactcgc gttgggtcgc ctacccgttc
      541 cacctgctcg actgctgcct agtgaccgat gccgcgggcg ccgtcatcgt cgcgagcccg
      601 gaacgagcgg ccaagatgaa gaagaagccc gtgtgggtgc tcggatgcgg cgaatcgaac
      661 acacaccagt cgatctcgca gatgccggat ctcacgcaaa caccggcgcg ccattccggc
      721 ccgcgagcct tcgagatggc gcagatgaaa cacgccgaca tcgatgtcgt cgagatctac
      781 gactcgttca cgtacacggc gctcgtgacg ctcgaggagc tcgggttttg caagaagggg
      841 gaggggggac ggttcgtatc cggccagcga accgcgcccg gcggcgactt tccgatgaac
      901 acgaacgggg gcggcctctc ctacacgcat cccggaatgt acggcatctt catcctcatc
      961 gaggcggcgc gtcagctgcg cggcgaatgc ggcgagcggc aggtgccgaa cgccaagacg
     1021 gcgctcgtca acggcacggg cggcgtcctt tcttccacgg gaaccgtgat tctcggcgtc
     1081 gattgaggga tcgccggccg ctatcagccg aagtatgtcg accagtgcag ggccgggcgg
     1141 atgacatgcc cggcccgctt cgtctacgat agcccacgcg acgcataccg cgtacgccat
     1201 ccggagggaa gactccatct atcgtgctga ttgacctgca ctcgcatacc tggccgctga
     1261 gcgatgacag ccttctcgac ccagatgacc tcatcatccg aaccaaggaa gccggcatcg
     1321 atggcatctg cctcaccgag cacgacttct tctgggacca cgagaaggtg cgccagctgg
     1381 cgcataagca ccagttcctc gtcctgcccg gttgcgaggt caatacggag cagggccata
     1441 tgctgacgtt cgggctcacc gagtaccggt tcggcatgca ccgtgtcgaa gagctcgccc
     1501 ggatggtgga tgcggtggac ggggcgatga tcgccgccca tccgtatcgc cgtcagatgc
     1561 cctggcagcc gaacgacccg aacgactacc atcgggcgct cgagcgagcg aagcagaatc
     1621 gcgcttatcg gtatgtcgcc gcattggaga tcgagaacgg gcgcggcagc gagcgtgaga
     1681 acgagttctc gcgccgcttg gcgacagagc tcgagatgcc ggggacggcc ggcaccgatt
     1741 cgcacgcgcg caaggacatc gccaggtgtg ccacatactt cgaggccgac atccggtcgc
     1801 tggaggatct catccaggca ctgaaggcgg gccgcttcta cccggtcacg cttcagcgag
     1861 caagggcata gcggttgccc caacgggggc gagggaggga gacatggccg aagagcaaga
     1921 atccgtcatc acggatgaga tgcgagcaca gatagggaag gagtccgagc ctgtcatcta
     1981 cgaggtcgac aagacgacga tccggctcta tgcccgcgcc gtcggctacg atgacccgct
     2041 gtactacgac gaggagttcg ccaggagcaa aggatatcgc tcgcttcccg cgccgcccgg
     2101 attcctcggg ttccccatct acgatccgcg caacgccggt cagcagatgg gaccgccgcg
     2161 gttccagact cgccttcagc gagtgctgaa cggcggcagc gagttcgagt acttcgacga
     2221 catctgcgcc ggcgatacac tcgtcgcgac cggccgtatc accgatatct acgagcgccc
     2281 tgggcgcatg ggactcatgc tcttcaccga gcgcgagcag gtgttcaagc gcgacggcaa
     2341 ggtcgtcgcg gtcagccgcg gacgcagcat caactactag cgccatcgac ggcgagtgct
     2401 gtgccgcaga ggcgcgacag cgaggggaga tggaaccatg gcgacggaaa ctcgtactca
     2461 gctctattgg gacgacgtgc aggaagggca ggagctaccc accctcagga agaagccgaa
     2521 cagccagcag ctcgtgctgt gggcggccgg ttcgggcgac ttctaccaga tccactacga
     2581 taaggatttc gcgaagtcga ccggcctgga caacatcatc gtgcacgggc tcctcaagaa
     2641 tgccttccag gggcagctcc tgcacgactg gatcggctac gaaggccgca tcaagaagtt
     2701 cggctgcagc tatcgaggca tggacccggt cgaccaggag atcctcgtga agggcgtcgt
     2761 gacgaagaag tacgaggcgg gcggcgagta tctcgtcgat gtggacgtgt ggacagagaa
     2821 ccctgccggc gagaagacga cgccgggcta cggcaccgtc cggttgccca ggcgctgaga
     2881 gtacagcttc catcagcacc cgaggcctcg gagcctgggc gccattcgaa gaaccgcgcg
     2941 gtgcgagaag gcacaaaagg agatcaccat gggccgactg gacgggaggg tggctctcat
     3001 cacgggaagc gggcgcggca tcggccgcgg tatggccttg ctgttcgcca gcgagggcgc
     3061 gaaggtcgtc gtgaacgacc ccggtgtgaa ca
//
LOCUS       DATMBB010000263         4049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_406432, whole genome shotgun sequence.
ACCESSION   DATMBB010000263 DATMBB010000000
VERSION     DATMBB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406432"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VNL92_05635"
     CDS             complement(<1..1818)
                     /locus_tag="VNL92_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWO94226.1"
                     /translation="MIDEPQAFGLYRREHEHDACGVGFVADVRGRPSHDILAKALEAV
                     ANLTHRGAVDAELTGDGAGIMSQLPKRFFAREAERLGVRVQRPDDLAVAMLFLPGREP
                     EAARKAEELLEGALAAHCIPLLAWRDVPVDASVLSPLGRRTQPLIRQAIVGRPDAASD
                     DHEYERVLYAARRDFEKRVQAAGLADAYVPSFSAHTIVYKGLMIAPQLPDFYEDLRDP
                     AFETAIALYHQRYSTNTFPNWYLAQPFRLLAHNGEINTRQGNQNWMRAREPEFHSSAW
                     GDRIAELTPIIQPGGSDSSDLDNVYEALVRSGRDPLHAMLLMVPEAWENMPSMDPKVR
                     AFYEYHACLMEPWDGPAALSFTDGRIVGAVLDRNGLRPSRFVLTEDGLLFAGSEVGAI
                     DLEDRVIVHKGRLGPGEMIAVDTERGVLLDNAAIKEAYSERAPYGEWLERRMVRLEGL
                     VSREAAAEAASKDVTSSALLPAQRAFGYTREELQLVLRPMVLEAKEAVGSMGDDTPLA
                     VLSHEPRLLYNYFRQKFAQVTNPPIDPIREQLVMSLLTTLGPRGSVFEEDEEHAHLLE
                     LRSPFLTEREMAVLRTLDDPKFRSATVSALFPVDDGAAGL"
     gene            complement(2038..2364)
                     /locus_tag="VNL92_05640"
     CDS             complement(2038..2364)
                     /locus_tag="VNL92_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HWO94227.1"
                     /translation="MTTQERTMPTNDEIMERLRTVEDPELRMSIVDLGLVYDVTPRPD
                     GTVRILMTLTSPGCPLGPVIGGQAYAALIDLPGVEDVDVEITFKPPWDPRTMASEEVQ
                     MMLGIY"
     gene            complement(2361..2834)
                     /locus_tag="VNL92_05645"
     CDS             complement(2361..2834)
                     /locus_tag="VNL92_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWO94228.1"
                     /translation="MTRNVELDELYRDIILDHYRKPRNRGRIDDSNATAEGLNPVCGD
                     QVRLQMHIEPEGRIDAIGFQGSGCSISQAAASIMTVSVKGKSVREASGMVRGVRSMLT
                     EGARPPEEAGDDIEALQGVAQFPARVKCAMLPWTVLEQALQEGPGQDDPAKEVST"
     gene            complement(2831..>4049)
                     /locus_tag="VNL92_05650"
     CDS             complement(2831..>4049)
                     /locus_tag="VNL92_05650"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWO94229.1"
                     /translation="DFPILSRQVHGRPLVYLDNAATTQKPQVVIDALVEYYTRHNANI
                     HRGLHRLAEEATEAYEATREHVARFIHAASSEEIVFTRNTTEAINLVAATWGRTNVGQ
                     DDEIVVTCMEHHSNLVPWQMLAQEKGARLRFIGVDGAGELRREDFDLIGERTKLVALA
                     HASNVLGTVNPVRELAERAHAAGALLLVDGAQSAPHMPVDVQALQCDFFAFSAHKMLG
                     PTGVGVLRARRDLLAEMPPFLGGGEMVSLVGDEAATWNALPWKFEAGTPNIADVVAFD
                     AALTYLEQLGMANVREHEEQIAAEAIHRLSELPGLTLHGPRDAARRGAAISFSLDGIH
                     PHDIGTILDQLGIAIRAGQHCAQPLMRVLGVPATARASFYVYNTFDEIDMLIEGLREA
                     QRIFAAPAEATLR"
BASE COUNT          663 a         1298 c         1359 g          729 t
ORIGIN      
        1 gagcccggca gcgccgtcgt cgaccgggaa gagcgccgaa accgtcgcgc tacggaactt
       61 cgggtcgtcc aacgtgcgca gcacggccat ttcgcgttcg gtgaggaacg gcgagcgcaa
      121 ctcgagcagg tgcgcgtgct cttcgtcctc ctcgaagacg ctgcctcgcg gcccaagcgt
      181 cgtcaggagc gacatcacga gctgctcgcg aatcggatcg atgggcggat tcgtgacttg
      241 cgcgaacttc tgccggaagt agttgtagag cagacgcggc tcgtggctca gcacggcaag
      301 gggcgtgtcg tcgcccatcg accctaccgc ttccttcgcc tccagcacca tgggccggag
      361 gaccagttgc agctcctcgc gcgtgtagcc gaaggcgcgc tgcgcgggca gaagagccga
      421 ggacgtgacg tccttgctgg cagcctcggc tgcggcctca cggctgacga gcccctccag
      481 acggaccatc cgccgttcca gccattcgcc gtagggtgcg cgctcgctat aggcctcctt
      541 gattgccgcg ttgtccagca ggaccccacg ttccgtatcg acggcgatca tctcgccggg
      601 gccgagccgg cctttgtgga cgatgacgcg gtcctcgaga tcgatggcgc cgacctccga
      661 gcccgcgaag agaaggccgt cttcggtgag gacgaagcga gaggggcgca agccattgcg
      721 gtccagcacc gcgcctacga tccggccgtc ggtgaacgag agcgcggccg gtccatccca
      781 cggctccatg agacatgcgt gatactcgta gaaggcgcgg accttcgggt ccatcgacgg
      841 catgttctcc cacgcctcag ggaccatgag gagcatggcg tggaggggat cgcgtccgga
      901 gcgcacgagc gcctcataca cgttatcgag gtcggacgag tcgctgccgc cgggctggat
      961 gatgggcgtc agttcagcga tccggtcgcc ccacgcggag gagtggaact ccggctcccg
     1021 agcccgcatc cagttctggt tcccctggcg cgtgttgatc tcgccgttgt gcgccaggag
     1081 gcgaaacggt tgggccaggt accagttcgg gaacgtattg gtgctgtacc gttgatggta
     1141 cagggcgatt gccgtctcga acgcggggtc gcgcaggtcc tcgtagaagt cgggcagttg
     1201 gggcgcgatc atcagcccct tgtagacgat cgtatgggcg gagaaggagg gaacgtaggc
     1261 atctgccagg cccgccgcct ggactcgctt ctcgaagtcg cgtcgcgcgg cgtagagtac
     1321 ccgctcgtac tcatggtcat cggaggccgc atcgggccgt ccgactattg cttgtcgaat
     1381 gaggggctgg gttcgcctgc cgagcgggct gagcacgctg gcatcgacag ggacatcgcg
     1441 ccaggcgagc aacggaatgc agtgcgcggc gagcgcgccc tccagcagtt cctcggcctt
     1501 gcgcgccgcc tccggctcgc ggccgggaag gaacaacatc gcgacagcga gatcgtccgg
     1561 acgttggacg cggacaccca agcgctcggc ctccctggcg aagaagcgtt tgggcagctg
     1621 gctcatgata ccggccccat cgccggtcag ttcggcgtcg acggccccgc ggtgtgtcag
     1681 gttcgcgacg gcttcgagcg ccttcgcgag gatatcgtgc gaagggcgcc cacggacgtc
     1741 ggcgacgaag ccgacgccac acgcgtcgtg ctcgtgctcc cttcgataga ggccgaaggc
     1801 ttgcggctca tcgatcaagg taatggcacc cctacccgcg cggcgcgcga tccctgtgaa
     1861 cgtgaccgat atgaggttgg ggaaggatgg ttcatgatag cacaatagat accgtcatga
     1921 cgttctcgcc gcaagtatat gtgaaaaatt ccaccaacac gagatcgtgc tggtggtgaa
     1981 cccatgtccg tctcgaggat cagatccggg aaagcggccc tgaacgctct aggctagcta
     2041 gtagatcccc agcatcatct ggacttcctc gctcgccatc gttcgcgggt cccacggcgg
     2101 cttgaacgtg atctccacgt ccacgtcttc cacgccaggc agatcgatga gggcggcgta
     2161 cgcctgacca ccgatgacag ggcctagggg acagccgggg ctcgtcaacg tcatcaggat
     2221 gcgcacggtt ccatcgggcc gcggggtcac gtcgtagaca agcccgaggt ctacgatgct
     2281 catgcgcagc tccggatcct ccaccgtgcg gagacgctcc atgatctcgt catttgtcgg
     2341 catggttcgc tcttgggttg tcatgtactc acctccttcg ccggatcgtc ttggcctggg
     2401 ccttcctgca gcgcctgctc gagcaccgtc caaggcagca tcgcgcattt gacgcgtgcc
     2461 ggaaactggg ctacgccctg cagcgcttcg atgtcgtcgc cggcctcctc gggcggccgc
     2521 gcgccctctg tcagcatcga ccgaacgccg cggaccatgc cgctggcctc gcgcacgctc
     2581 ttgcccttca cgctgaccgt catgatggaa gcggccgctt ggctgatgga gcatccgctc
     2641 ccctggaatc cgatggcgtc gatacgccct tcgggctcga tgtgcatctg aagccggacc
     2701 tgatcgccgc agacggggtt caatccctca gcggtggcgt tcgagtcgtc gatccgacct
     2761 cgattcctcg gtttgcggta atggtcgagg atgatgtcgc ggtagagctc gtccagctca
     2821 acgttcctgg tcatcgtagc gtcgcctctg cgggggcggc gaagatgcgt tgcgcctcgc
     2881 gcagcccctc tatcagcata tcgatctcat cgaatgtatt gtagacgtag aagctggctc
     2941 gagcggtcgc cggaacgccg aggacgcgca tgagcggctg ggcgcaatgc tgcccggccc
     3001 gaatcgcgat cccgagctgg tcgaggatcg taccgatgtc gtgcgggtgg atgccatcaa
     3061 ggctgaagga gatggcggcg ccgcgacgcg cggcgtcgcg tggcccgtgg agggtgaggc
     3121 cgggcagctc gctcaaccgg tgtatggcct ccgcggcgat ctgctcctcg tgctcccgga
     3181 cgttcgccat gccgagctgc tcgaggtagg tgagcgcggc gtcgaaagca acgacgtcgg
     3241 cgatgttcgg cgtgccggcc tcgaacttcc agggcagcgc attccaggtc gccgcctcgt
     3301 cgccgaccag gctcaccatc tcgcctcctc cgaggaaggg cggcatctcg gcgaggaggt
     3361 cacgtcgcgc ccggagcacg ccgacccctg tcggaccgag catcttgtgt gcggaaaagg
     3421 cgaagaaatc gcactgcagc gcctgcacgt cgacgggcat gtgcggagcg ctctgtgcgc
     3481 cgtccacgag gagcagcgca ccggcggcat gggcgcgctc ggccagctcc cggaccgggt
     3541 tgaccgtgcc gagaacgttg gaggcatgcg cgagcgccac gagcttcgtt cgctcgccga
     3601 tgaggtcgaa gtcctcgcgg cgcagctctc cggctccatc gacgccgatg aatcggagcc
     3661 gggccccctt ctcctgggcg agcatctgcc acgggacgag attcgagtgg tgttccatgc
     3721 atgtcacgac gatctcgtcg tcctggccca cattggtgcg tccccacgtc gccgcgacga
     3781 ggttgattgc ctccgtagtg ttgcgcgtga acacgatctc ctcggagctc gcggcatgga
     3841 tgaagcgtgc gacgtgctca cgcgtggcct cataggcctc cgtggcctct tccgcgagac
     3901 gatgcaggcc gcgatggatg ttcgcgttgt gccgggtgta gtactcgacg agcgcgtcga
     3961 tgacgacctg tggcttctgg gtcgtcgcgg cgttgtccag gtacaccagc gggcgtccgt
     4021 ggacctggcg gctcaggatg gggaaatcg
//
LOCUS       DATMBB010000264         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_247362, whole genome shotgun sequence.
ACCESSION   DATMBB010000264 DATMBB010000000
VERSION     DATMBB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247362"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VNL92_05655"
     CDS             complement(<1..1285)
                     /locus_tag="VNL92_05655"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWO94230.1"
                     /translation="MTYTKSIELAGKQLTIETGKLAQQADGAVTVRYGDTVILATAVA
                     SKTAREGIDFFPLTVEYEERHYAVGRIPGSFFRREGRPTTEAILTARLVDRPIRPLFP
                     KGFRNEVQIIVTTLSTDQENDPDVLAILGASAALSISDIPFGGPVAGVRIGRINGRYV
                     VNPTFSERDDSDLDLVVAGTADAIMMVEAGASQVEEAVMLEGLRVATEAIREMVELQE
                     DLVAEVGRPKREIEVRLPDPAIEEAVRAYVADDLLHAVSTVAKQERNDEIAALRNRTL
                     EHFLEQYSPIDVSSAFEEQLQRAIRGRILELGARPDGRRPDEIRPISCEVGLLPRTHG
                     SGLFSRGETQVLTMATLGSKGDAQRLDTLEPISSKRFMHHYNFPPFSTGETGRVGSPR
                     RREIGHGALGERALEPVIPPEEDFPYTIRLVSEVLS"
     gene            complement(1505..1771)
                     /gene="rpsO"
                     /locus_tag="VNL92_05660"
     CDS             complement(1505..1771)
                     /gene="rpsO"
                     /locus_tag="VNL92_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033016867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWO94231.1"
                     /translation="MVVTEAKSEIIEKYRRGPDDTGSPEVQIAVLTKRINHLNEHLRT
                     HKHDHHSRRGLLQMVGRRRRLLAYLNRTDVSRYRAIIAALGLRR"
     gene            complement(1869..>2430)
                     /locus_tag="VNL92_05665"
     CDS             complement(1869..>2430)
                     /locus_tag="VNL92_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWO94232.1"
                     /translation="RVSEMGLERAKRLVEGGQDVVILMDSITRLSRAYNLAMPSSGRT
                     LSGGMDPIALYPAKRFFGAARNTEEGGSLTIIATCLVDTGSRMDEVIYEEFKGTGNME
                     LHLERRLAERRIFPAIDVPRSGTRREELLFDEGTLRQVYLLRRMISLIGANSTNPSEP
                     TERLLERLSKTKNNEEFLATLSKQEV"
BASE COUNT          387 a          746 c          822 g          475 t
ORIGIN      
        1 tcgagagcac ctccgatacg aggcggatgg tataggggaa atcctcttcg ggcgggatca
       61 cgggctcgag tgctcgttca cccagcgctc catgacctat ctccctacgc cgaggcgagc
      121 ccacccgtcc cgtctcgcca gtggagaagg gcgggaagtt gtagtggtgc atgaagcgct
      181 tgctcgagat gggttctagc gtgtccaggc gctgtgcgtc gcccttggag ccgagggtgg
      241 ccatggtgag tacctgggtt tcgcctcggc tgaacaagcc ggagccatgg gtgcgcggca
      301 gcaatccgac ttcgcaggag atgggacgga tctcgtccgg acggcggccg tccggacgcg
      361 cgccgagctc gaggatgcgg ccccggattg ctcgctgcag ctgctcctcg aaggccgacg
      421 agacatcgat aggcgagtac tgctcgagga agtgctcgag ggtgcggttg cgtagcgccg
      481 cgatctcgtc gttgcgctcc tgcttcgcga ctgtcgacac cgcgtgcagc aggtcgtcgg
      541 ccacgtaagc tcgcacggcc tcctcgatgg ctggatcggg cagacggacc tcgatctccc
      601 gcttggggcg tcccacctcg gctacgagat cttcctgcag ctccaccatc tcgcggatcg
      661 cttccgtggc tacccgcaag ccctccagca tcaccgcttc ctcgacctgc gaggcgcctg
      721 cctccaccat catgatcgcg tcggcggtgc cggctaccac gaggtcgagg tcgctgtcat
      781 cccgctcgga gaaggtgggg ttgacgacgt agcggccgtt gatgcggccg atgcggaccc
      841 cggcgacagg gccgccgaac gggatgtcgg agatcgagag cgccgcggat gcgccgagta
      901 tggcgagaac gtcagggtcg ttctcctgat ccgtggagag cgtggtgacg atgatctgca
      961 cctcgttgcg aaagcccttg gggaagagcg gccgaatcgg ccggtcgacg aggcgcgccg
     1021 tcaggatcgc ttccgtcgtc ggacgtccct cgcgccgaaa gaagctgccc ggtatgcgcc
     1081 cgacggcgta gtgtcgctcc tcgtactcaa cggtcagggg gaagaagtcg ataccctccc
     1141 gcgccgtctt gctcgcgacg gccgtcgcca ggatgacggt atctccgtag cgtaccgtca
     1201 cggcgccgtc ggcttgctgg gcgagcttgc ctgtctcgat ggtgagctgc tttcctgcaa
     1261 gctcaatgct ctttgtgtag gtcatagcgg actcctcgtc gtgggcggcg cgtggaaggc
     1321 gttatctcga tgccctgagg cgtacctcag tagcaagggg gcctcttggc gagcctgtgc
     1381 gtagctgact ggtggatagt ggaggccatc ccctcggggc ggcgcaaatc cgtctcgcat
     1441 tggggctcgc cggtggttct ccctctcaca cccgtacgcg ccagatagcg aaaggcctcc
     1501 gcgattatcg tcggaggccc agtgcggcga tgatggcccg ataccggctc acgtcggtcc
     1561 ggttgaggta agccaagagg cgccgccgcc gtccgaccat ctgcagaagg ccgcgcctcg
     1621 agtgatggtc gtgcttgtgc gtgcgtaggt gctcgttcag gtgattgatt cgcttcgtga
     1681 gcacggctat ctgaacctcg ggtgaaccgg tgtcatccgg accgcggcgg tacttctcga
     1741 tgatctcgga cttggcctct gttaccacca tgaaccctgc ttcgtagtag ttatgcccag
     1801 tctagcacgt gcccgaacgg gtgggcaatt tgtcgggatg gatggacttg cgcacctgcc
     1861 agatgaatct acacttcttg cttgctgagg gtggcgagga actcctcgtt gttcttggtc
     1921 ttcgagaggc gctccaacag tcgctctgtg ggctcggagg gattggtgga gttggcgccg
     1981 atgagcgaga tcatgcgccg cagcaggtac acctggcgga gcgtcccttc gtcgaagagg
     2041 agctcctccc ggcgcgttcc ggagcgggga acgtcgatag cggggaagat gcgccgctcc
     2101 gcgagccgcc tttcgaggtg cagctccatg ttgccggtgc ccttgaactc ctcgtagata
     2161 acctcgtcca tgcggctgcc cgtatcgacg aggcaggtag cgatgatggt gagactgcct
     2221 ccctcctcag tgttgcgggc ggcgccgaag aagcgcttgg cggggtacag ggcgatggga
     2281 tccataccac cggaaagcgt gcgcccgctg gagggcatcg ccaggttata agcgcgggag
     2341 agccgcgtga tgctatccat gaggatgacg acgtcctgcc cgccctcgac gagacgcttg
     2401 gctcgctcca gccccatctc gctcacgcgc
//
LOCUS       DATMBB010000265         3476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_123354, whole genome shotgun sequence.
ACCESSION   DATMBB010000265 DATMBB010000000
VERSION     DATMBB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123354"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VNL92_05670"
     CDS             complement(<1..255)
                     /locus_tag="VNL92_05670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWO94233.1"
                     /translation="MTQPYPELLGRWAELKKEWEAIPFWRLLGMEATGLDEGRCRIRL
                     GVRPDLLAPGDVLHGGFYGCLIDTAVGSALKCYRELDRPEV"
     gene            complement(252..1118)
                     /gene="folP"
                     /locus_tag="VNL92_05675"
     CDS             complement(252..1118)
                     /gene="folP"
                     /locus_tag="VNL92_05675"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWO94234.1"
                     /translation="MVAAALESQSSPAPIRIGPRVFTWSARTYVMGILNMTPDSFSGD
                     GLALRGIEAALETARRMVEAGADMLDVGGESTRPGAEPVGAEEEMRRVVPLIEALAKA
                     LPDVPISIDTWKAEVARAALQSGAHWVNDVWGFLGDSNMANVVAEHDVPCVLMFNGRG
                     VKVHDVLNDAKAALRRSMEAAREAGVPADRIVLDPGFGFGITAEQSLELLRRLRELRE
                     LGRPLLAGTSRKSMIGAVLGLPVEARLEGTAATVALAIANGADIVRVHDVQEMTRVAR
                     MADAIVRGWRPS"
     gene            complement(1121..1528)
                     /locus_tag="VNL92_05680"
     CDS             complement(1121..1528)
                     /locus_tag="VNL92_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWO94235.1"
                     /translation="MFELPQSYYESIVAHAREDAPNECCGILAGDDGRVVKLYRATNI
                     EHSPYRYNVDPKELFHIYREIEERSWDLLGIYHSHTATEAYPSPTDVRLAGWPDAIYL
                     IVSLRDAAPVLRAFRINDGHVTEEELRIVEEAR"
     gene            1677..2273
                     /locus_tag="VNL92_05685"
     CDS             1677..2273
                     /locus_tag="VNL92_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94236.1"
                     /translation="MPKRLTQHRIIAGLSLVVLVMLLALAVACGDDDDDDDDDDGATA
                     VATTERTAEATEPADADDDEDEASPTADAGDVDPEVAAYAGQFCGEFDSFLQEILAVP
                     GLAEAETLTDEQAAVFEGLANDFADGLAALDPPGQFGDWQDEFVAAIRDNAGDAEALL
                     MEVELPDPPEEAQADFEAALQTEPACLDLQETISSGGP"
     gene            2369..2935
                     /locus_tag="VNL92_05690"
     CDS             2369..2935
                     /locus_tag="VNL92_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94237.1"
                     /translation="MTRVTRYRLVPLLGLLACALLFTLAVACGDDDDDDDDDENGATA
                     VATSEPTEEGTPDATATGEAGTTDIDPDVEQYVASFCTEFDQLFTDLSTTAADAGAGS
                     LTEEEAFAEIEQAINAFADRMDALDPPPSLQQWQDDFVALLRENAADPEAFSNLEPPE
                     PPADVADQFNAAGETIPECQALSSAIGA"
     gene            2999..>3476
                     /locus_tag="VNL92_05695"
     CDS             2999..>3476
                     /locus_tag="VNL92_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWO94238.1"
                     /translation="MTASSTRRRVLVVEDDETLRESLRYTLSRDGYEVVAAADGPAGL
                     RVAREDEPDLVILDLMLPGLDGLEVCRQIRRSSQVPILMLTAKREEVDRVVGLEIGAD
                     DYVAKPFSMRELLARVRALLRRSVYGAQQATEKPTVVRSGDMTIDLGRRETRIGERV"
BASE COUNT          617 a         1139 c         1172 g          548 t
ORIGIN      
        1 cacctcaggc cgatcgagct cgcggtagca cttgagcgcc gagcctaccg ccgtgtcgat
       61 caggcatccg tagaagcctc cgtgcaacac gtcgccgggc gccaacaggt ccggccgaac
      121 cccgaggcga atccgacaac gtccctcgtc gagccctgtc gcctccatgc cgagcagccg
      181 ccagaaggga atggcttccc actccttctt cagctccgcc cagcgaccaa gcaactcggg
      241 gtagggttga gtcacgaggg ccgccagcct cggacgatgg catcggccat gcgcgctacg
      301 cgcgtcatct cctgtacgtc gtgtactcgc acgatgtcgg cgccgttcgc gatggcgagc
      361 gccacggttg ccgcggtccc ctcgagccgc gcctcgacag ggaggccgag gacggctccg
      421 atcatcgact tccgcgatgt tccggcgagg agcgggcgtc cgagctcgcg gagctcgcgg
      481 aggcgtcgca gcaattcgag gctctgctcg gctgtgatgc cgaagccgaa ccccggatcg
      541 agcacgatgc ggtccgcggg cacgccggcc tctcgcgccg cttccatcga gcgccgcagg
      601 gccgccttcg catcgttaag gacgtcgtgc accttcaccc cgcgcccgtt gaacatgagc
      661 acgcagggca cgtcgtgctc cgccaccacg ttcgccatgt tcgaatcgcc caggaatccc
      721 caaacgtcgt tgacccagtg ggccccgctc tggagcgcgg cgcgcgcgac ctcagccttc
      781 cacgtatcga tgctgatggg cacatcgggc agcgccttcg cgagggcttc gatgaggggt
      841 acgacccgcc gcatctcctc ctccgcgccg accggctccg caccggggcg ggtcgattcc
      901 ccgcccacgt cgagcatatc ggcgccagct tccaccatcc ggcgcgcggt ctccagggca
      961 gcttcgatgc cgcgaagggc caggccatcg cccgagaagg agtcgggcgt catgttgagg
     1021 atgcccatca catacgtccg cgcactccag gtgaaaaccc gtggcccaat gcggatgggg
     1081 gcgggagaag actggctctc cagcgcggcc gcgaccacgg ctaccgtgcc tcctcgacga
     1141 tgcggagctc ctcttccgtc acgtgaccgt cgttgatgcg aaaggcgcgc agtacgggag
     1201 cggcatcgcg caaggagacg atgaggtaga tcgcatcggg ccagccggcg aggcgcacat
     1261 cggttggcga agggtacgcc tccgtcgcgg tgtgcgagtg gtagatgccg aggaggtccc
     1321 acgagcgctc ctctatctcc cgatagatgt ggaacagttc ctttggatcg acgttgtagc
     1381 ggtaagggct gtgctcgata ttcgtggcgc ggtacagctt gacgacccgg ccatcgtcgc
     1441 cggccagaat gccgcagcac tcgttcggcg catcctcccg cgcgtgcgca acaatggatt
     1501 cgtagtagga ctgagggagc tcgaacacgc gaacctcgga tgctcacgcc gtcacaccgg
     1561 caccgtcccc acgagtgtag cagaaggcgt tgggagcgga gttgcctttg gcggagcccg
     1621 tcggttgcct caaggcgtca ggtagggtag gaatgaaagg acgaaaggag gctctcttgc
     1681 caaagcgcct tacccaacac cggatcattg cgggactcag cctcgtcgtt ctcgtgatgc
     1741 tgctcgccct cgcggttgca tgcggcgacg acgatgacga cgatgatgac gacgatggcg
     1801 ccaccgccgt ggcgacaacc gagcgaaccg cggaggcgac tgagcccgcg gacgccgatg
     1861 atgacgagga cgaagcttct ccgacggcag atgccggcga cgtggatccc gaggtagcgg
     1921 cctacgccgg gcagttctgc ggcgagttcg actcgttcct tcaggagata ctggccgttc
     1981 ccggactggc cgaggccgaa acccttaccg acgagcaggc cgccgtgttc gaagggctcg
     2041 cgaacgactt cgcggatggg ctggctgcgc tggacccgcc agggcagttc ggagattggc
     2101 aggatgagtt cgtcgcggcc attcgcgaca acgccggcga cgcggaagca ctcctgatgg
     2161 aggtcgagtt gcccgacccg cccgaagagg cgcaggccga tttcgaagcc gcactgcaaa
     2221 ccgagcccgc gtgcttggac ctgcaggaga ccatctcctc aggcggaccc tagatgctgc
     2281 gtttcgtgac ccgcgctaag tcgggacgca tgggccatcg aagccgcgaa ccttggggcg
     2341 agcgagcaca tccgaggagg ccggaaccat gactcgcgtc acgcgatatc gcttggtgcc
     2401 cttgctgggg ctcctggcgt gcgcactcct gttcacactt gcggtcgcat gcggcgacga
     2461 tgatgacgat gatgatgacg acgaaaacgg ggcgactgct gtcgccacga gcgagccgac
     2521 cgaggagggt actcccgacg cgaccgccac gggagaagcg gggacgactg acatcgaccc
     2581 cgatgtggag cagtatgtcg ctagcttctg caccgagttc gatcagctgt tcaccgacct
     2641 ctcgaccacg gcagccgatg ccggcgctgg gagcctgact gaggaagagg cgtttgcgga
     2701 aatcgaacag gcgatcaacg cgttcgccga tcggatggac gcgctcgatc cgccgccctc
     2761 actgcagcag tggcaagacg acttcgtcgc actgctgcgg gagaacgccg ccgacccgga
     2821 ggccttcagc aacctggagc cgccggagcc gcctgcggac gtggccgacc agttcaacgc
     2881 cgcgggcgag acgattcccg agtgccaggc actgagcagc gctataggag cgtgacggga
     2941 gcgatccgag ggggccttcg ccgagcggca tggggcgccc atcacctata atggcgccat
     3001 gaccgcgtca tccacgcgac ggcgtgtcct tgtcgtcgag gacgatgaga cgctgcgtga
     3061 gtctctgcgc tacacgctgt ctcgcgacgg ctacgaggtc gtcgccgcgg cggatggacc
     3121 cgcagggctg cgagtcgcgc gcgaggacga gccggacctg gtcattctcg accttatgtt
     3181 gccggggctg gacggcctcg aggtgtgccg gcagattcga cgctcgtcgc aagtaccgat
     3241 cctgatgctg acggcgaagc gggaagaggt cgacagggtc gtcggcctcg aaatcggagc
     3301 ggacgactac gtagcgaagc cgttcagcat gcgcgagctg ctcgcgcgcg ttcgggcgct
     3361 gctgcgccgg tccgtatatg gcgcccagca ggccaccgaa aagcccaccg tcgtgcgcag
     3421 cggcgacatg acgatcgacc tgggccggcg ggagactcgc atcggcgagc gagtgg
//
LOCUS       DATMBB010000266         6238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_142249, whole genome shotgun sequence.
ACCESSION   DATMBB010000266 DATMBB010000000
VERSION     DATMBB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142249"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1732)
                     /locus_tag="VNL92_05700"
     CDS             complement(74..1732)
                     /locus_tag="VNL92_05700"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWO94239.1"
                     /translation="MAGKPVRVIPLGGLGEIGRNCMIIEYGDDMIMIDAGLMFPEEEM
                     LGVDLVIPDITYVLQNRRKLRGIFMTHGHEDHIGALPYLLRQIQAPIYATKLTHGLIE
                     VKLKEARLLRETELCMVEPEDEIDIGAFTVEFMRVAHSIPDACGIIVRTPEGTIIHTG
                     DFKIDHTPVMGHYTDLPRLAELGDEGVLLLCSDSTYAEVPGYTPSEQVVGEALDRMIA
                     TAPGRVIISTFASLIARIQQVIDAAERHGRKVFVTGRSMIDNVHMAQELGYLKANTGT
                     ILRVEEMRRLAPEKLVVVSTGSQGEPTSALVRMANQDHPHIKIVPGDTIIMSSTPIPG
                     NDALVDRVIDNLFKLGARVLYSKVASVHVRGHAAQEELKLILRLVRPRFFMPIHGEYR
                     HLVHHADLATQMGLPRENVFVLEDGDVLELDRDQAYLEERVPASYVYVDGLGVGDVDH
                     VVLRDRWHMATDGTVVVVVTMDKQTGQVVGLPEVVWRGFVDAEESEELLQRTREAVAA
                     SLNGGGGQRVDWSMVHGQMKDSVARFLWEQTHRRPMVLPVAVEV"
     gene            complement(1768..2397)
                     /locus_tag="VNL92_05705"
     CDS             complement(1768..2397)
                     /locus_tag="VNL92_05705"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWO94240.1"
                     /translation="MIQEATRAGELAALHRAIATCTKCALSGGRTQAVPGEGPWNADV
                     MFVGEGPGFHEDRQGRPFVGRAGQLLDEMLGVAGLQRDEVFITNIVKCRPPENRDPLP
                     NEIAACADYLTRQIELIGPKFIVTLGRYSMARFFPGQSISRIHGRQQTIGGITYVPMY
                     HPAAALRSAAVHEALETDFRRLGEMLREHARRELERQEPEPSAQQLRLI"
     gene            complement(2394..2789)
                     /locus_tag="VNL92_05710"
     CDS             complement(2394..2789)
                     /locus_tag="VNL92_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWO94241.1"
                     /translation="MPDFAVIAMVGIVGGWLAGMLGVGGGVIFVPALVLLLDHDQHEA
                     QGISLVVIAVTALAGTVVHRRQGNIDVPVAALVTPAAMVAGLVGGILATQLPEDALQR
                     IFAIMLAALSIRMSVQAWRGSPARSEVRP"
     gene            complement(2801..3220)
                     /locus_tag="VNL92_05715"
     CDS             complement(2801..3220)
                     /locus_tag="VNL92_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWO94242.1"
                     /translation="MRLLPSCAGCTIGPSMDDMQMRSRVVRSVATGVSGGLFSGLVGG
                     GGGVVMIPLLTGFLGMSQHRAHGTSLAIIILVAAAGAMPYVVSGHVDWGLALGLGVTS
                     TVAAMVSARAMVAMPDVWLRRAFALVLLAAAVRMLLG"
     gene            complement(3229..3957)
                     /gene="tatC"
                     /locus_tag="VNL92_05720"
     CDS             complement(3229..3957)
                     /gene="tatC"
                     /locus_tag="VNL92_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWO94243.1"
                     /translation="MTILEHLIELRNRVVVSAVAVVICTVITLVFAERIIDFLLEPAK
                     ESIDEEDFTLIFTKPFGYVGSYFRVGLTGGVALAMPILVYEALMYVSPALTKEEKRWV
                     FPTVLGAAAAFLGGAAFAYYIVMPPSMDFLLNFGEGTAEPFLEIGPYIDFVTRLLLAV
                     GLSFETPLIIMFLARIGLVSARKLLGWWRYAIVLAFVVSAVITPTIDPVTQLFVAGPI
                     IVLYGLGIVLAAIFGRTREVLQPR"
     gene            complement(4036..4596)
                     /gene="tatB"
                     /locus_tag="VNL92_05725"
     CDS             complement(4036..4596)
                     /gene="tatB"
                     /locus_tag="VNL92_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HWO94244.1"
                     /translation="MPNFDVFGVGFLEILVVLVLALIVIGPQRLPEVAVQLGRAVGQM
                     RRYANQFRREFMEEFGDIQAEWEMSQKEYREVQRQLSDVSVDVRSIDRDVKDATREVN
                     AALRPPIESSSRPKSDETLPSNVVPLDDAATQWRDGEAAGPPPEGEPSAVEDASPIPS
                     PGDEKDAIAAVREAMGSAPRPKLQRH"
     gene            complement(4689..4949)
                     /gene="acpP"
                     /locus_tag="VNL92_05730"
     CDS             complement(4689..4949)
                     /gene="acpP"
                     /locus_tag="VNL92_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWO94245.1"
                     /translation="MATVFERVQKIIVEQLGVDEDQVTPTTSFVDDLNADSLDLVELI
                     MSMEEEFSKDGVELEISDEEAEKITTVQDAVTYLKEHGIEDS"
     gene            complement(5018..5440)
                     /gene="nusB"
                     /locus_tag="VNL92_05735"
     CDS             complement(5018..5440)
                     /gene="nusB"
                     /locus_tag="VNL92_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWO94246.1"
                     /translation="MAGPARRQARTLALQALYESDTTGHSADAALARMLEAEEVNAPT
                     RELAESLLRGVNMHRSEIDRLLAEAAPQRPVEDIAAIDRNVLRLAIYEVVFDNRAPVA
                     AVLNEAVALAKRFGSESSGRFVNGVLRTIAGSATGQSR"
     gene            complement(5455..6198)
                     /gene="fabG"
                     /locus_tag="VNL92_05740"
     CDS             complement(5455..6198)
                     /gene="fabG"
                     /locus_tag="VNL92_05740"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWO94247.1"
                     /translation="MSLAGHVALVTGGSRGIGRAVSLLLAQRGAAVAVNYKTREDAAR
                     EVVDAIVSAGGRAIAIGGDVAVRTDVERIVREAEARLGVTDILIANAGLTHDDLLLRM
                     SEDDWDTVIDTNLRGTFLCTKAVLRGMMRRRWGRIIAISSVAGIIGNAGQANYAAAKA
                     GVNGFIRSVAKEMGSRGITANSVAPGFIDTEMTTGLSRDLREQALSVMAIRRFGTVDD
                     VAELVAFLASDAASYITGQVLAVDGGISL"
BASE COUNT         1134 a         2005 c         1982 g         1117 t
ORIGIN      
        1 tctcctcacg agccttgctc cagtcggacc ccgccacgac atccgaccgg cagatacaag
       61 cggctgtggt tcgctatacc tccacggcta cgggcaacac catcggccgc cgatgggttt
      121 gctcccacag gaagcgcgcc acgctgtctt tcatctgtcc gtggaccatg ctccagtcga
      181 cacgttgccc tccgcctcca ttcagcgacg ccgcgacggc ttcgcgcgtg cgctgcaaca
      241 gctcctccga ctcctcggcg tccacgaatc cacgccacac gacctcagga agcccgacca
      301 cttggcccgt ttgtttatcc atcgtgacga caaccaccac cgtgccatcg gtcgccatgt
      361 gccagcggtc gcgcagcacg acgtggtcga catcgccgac gccgaggcca tcgacgtaga
      421 cgtaactcgc cggcactcgc tcttcaagat acgcctgatc gcggtctagc tccagcacgt
      481 cgccatcctc gaggacgaag acgttctcgc gcgggaggcc catctgggtc gccaggtcgg
      541 cgtggtggac cagatggcgg tactcgccgt ggatcggcat gaagaatcga ggacggacga
      601 gacgcaggat gagctttagc tcctcctgtg cagcatgccc gcggacgtgc acactcgcga
      661 ccttgctgta cagcacacgg gcgccgagct tgaagagatt gtcgatcacg cggtcgacga
      721 gggcgtcgtt accggggatg ggggttgacg acataatgat cgtgtctccg ggcacgatct
      781 tgatgtgcgg gtggtcctgg ttcgccatgc gtacaagcgc tgatgtgggc tctccttggc
      841 tccccgtcga gacgacaacg agcttctcgg gcgccagccg gcgcatctcc tcgacgcgga
      901 ggatcgtgcc agtgttcgcc ttcaggtagc cgagctcctg cgccatgtgg acgttgtcga
      961 tcatgctgcg gccggtgacg aacaccttcc gcccatgccg ctcggcggcg tcgatgacct
     1021 gctggatgcg agcgatgagt gacgcgaagg tggagataat cacgcggccg ggtgcggtcg
     1081 cgatcatccg atcgagcgcc tcgcccacga cctgctcgga aggagtgtag cccgggacct
     1141 ccgcgtacgt ggagtcggaa cagaggagca gcacgccttc gtcccccagc tcggcgagcc
     1201 gaggcaggtc ggtgtagtgg cccatcacgg gcgtatggtc gatcttgaaa tcgccggtat
     1261 ggatgatcgt tccctccggc gtgcggacga taatgccaca cgcatccggg atgctgtggg
     1321 cgacacgcat gaactcgacc gtgaaggcgc ctatgtcgat ctcgtcctca ggctccacca
     1381 tgcacagctc tgtctcccgc aggaggcgcg cttccttgag cttcacttcg atcagcccgt
     1441 gggtcagctt cgtggcatag atgggcgcct gaatctgccg gaggaggtag ggaagggccc
     1501 cgatgtggtc ctcgtggccg tgggtcatga agatgccccg gagcttgcgt cggttctgga
     1561 gcacgtaggt gatgtcgggt atgacgaggt cgaccccgag catctcttcc tcggggaaca
     1621 tgaggccggc gtcgatcatg atcatgtcgt cgccgtattc gatgatcatg cagttccggc
     1681 cgatctcccc gagaccgccg agtggtatga ctctaactgg ctttccggcc atggatcctc
     1741 gtctactttg ggactagcga cataatacta gattagccgc agttgctgtg cgctcggttc
     1801 cggctcctgt cgctcgagct cgcgacgcgc atgctcgcga agcatctcgc ctagccgccg
     1861 aaagtcggtt tcgagagctt cgtgcacggc ggccgagcgg agagcggctg cgggatggta
     1921 catgggaacg taggtgatcc cgccaatcgt ctgttgcctg ccatggatac gcgagatcga
     1981 ttggcctggg aagaaccgag ccatcgagta ccggcccaac gtgacgatga actttgggcc
     2041 aatcagctcg atttgccggg tcaggtagtc agcgcatgcg gcgatctcgt tcgggagggg
     2101 gtcgcgattc tcaggcgggc ggcatttcac gatgttcgtg atgaacacct cgtcacgctg
     2161 cagcccggcg acgcccagca tctcgtcgag cagctgaccg gcgcgaccaa cgaagggccg
     2221 gccctgccgg tcttcgtgga aacccgggcc ttcgccaacg aacatgacgt ccgcgttcca
     2281 cgggccctcg ccggggacgg cctgcgtgcg tccaccgctg agcgcgcact tcgtgcaggt
     2341 cgcgatggcc ctgtggaggg cggcgagctc gcctgctcga gtggcttcct ggatcacggg
     2401 cgcacctccg atcgcgctgg ggagcctctc caggcctgca cgctcattct gatactcaat
     2461 gctgccagca tgatagcgaa gatgcgctgc agcgcgtcct ccggcagctg tgtcgcgagg
     2521 atccctccca ctaggcccgc gaccatggcg gccggcgtga cgagcgccgc cacgggcaca
     2581 tcgatgttgc cctggcgacg gtgcacgacc gtccccgcga gcgccgtgac ggcgatgacc
     2641 acgagcgaga tgccctgcgc ctcgtgctgg tcgtgatcga gcagtagcac gagagcgggc
     2701 acgaaaatca ctccgccccc gacacccagc atgccggcga gccatccgcc cacgattccg
     2761 accatggcga tgaccgcgaa atctggcacg cctgccgccc ctaacctagc agcatccgca
     2821 ctgcggccgc gagaagcacg agcgcgaagg cccgccgcag ccagacatcg ggcatggcca
     2881 ccatggctcg ggcgctgacc attgcagcta ccgtgctcgt cactcccagt ccgagcgcga
     2941 gcccccaatc gacgtgaccg ctcacgacat acggcatcgc gccggcggcc gcgacaagaa
     3001 tgatgatcgc gagggacgtg ccgtgagcgc gatgctggga catgccgagg aatccggtca
     3061 gcaagggaat catgacgacg ccgccacccc cgccgacgag gccactgaac agacctcctg
     3121 atacgccggt tgccaccgac cgaactaccc gtgaccgcat ctgcatgtcg tccatggatg
     3181 ggccgatggt acaccctgca caggaaggga gcaagcgcat aatcgtcttt accgaggttg
     3241 caacacctcg cgagtgcgcc cgaagatcgc ggcaagaacg atgcccaagc cgtagagcac
     3301 gatgatggga cccgccacga agagttgcgt gacggggtcg atggtgggtg tgatgacagc
     3361 cgatacgaca aacgcgagca cgatggcgta tcgccaccaa cccagaagct tgcgcgcgga
     3421 gacgagacca atccgcgcca agaacatgat gatgagggga gtctcgaacg acagtcctac
     3481 ggcgaggagc agccgcgtca cgaaatcgat gtagggtccg atttcgagga agggctcggc
     3541 ggtgccttcg ccgaagttga ggaggaagtc catcgagggc ggcatcacga tgtagtaggc
     3601 aaacgcggcg ccgccgagga aggccgcggc tgccccgagt accgtgggaa agacccaccg
     3661 cttctcttcc ttcgtcagcg cgggcgagac gtacatgagg gcttcgtaca cgaggatggg
     3721 catcgcgagg gcaacgcctc ccgtcagtcc gactcgaaag taggagccga cgtagccgaa
     3781 cggcttcgta aagatgaggg taaagtcttc ctcgtcgatg ctctccttgg ccggctcgag
     3841 caggaagtcg atgatccgct ccgcgaacac gagggtgatg acggtgcaga tcacgacggc
     3901 gaccgcggag acgacgacgc ggttccgaag ctcgatcagg tgctcgagga tcgtcatttc
     3961 ccccggggct gggggagctt cctcttcgac gttcggggtt ggctgacgca aggtcatggc
     4021 gcggccgggc agggactagt ggcgctgcag cttgggtcgc ggcgccgatc ccatcgcttc
     4081 acgtacggcg gcgatggcat ccttctcgtc gccgggcgag gggatcgggg atgcgtcctc
     4141 gaccgcggac ggctcgcctt cgggcggtgg cccggcagcc tccccatctc gccattgtgt
     4201 agccgcatcg tcgagtggaa cgacgttgga cggcaaggtc tcgtctgact tggggcggct
     4261 gctgctttcg atgggcggac gcagggcggc gttgacctcc cgcgtggcat ccttgacgtc
     4321 gcggtcgatg gaccgcacgt cgacggacac gtcggagagc tgacgctgga cttcgcggta
     4381 ctccttctgc gacatctccc actcggcctg gatatcgccg aactcctcca tgaattcccg
     4441 gcggaactgg tttgcatatc ggcgcatctg gccgaccgcg cgtcccagtt ggaccgcgac
     4501 ttcgggcagc cgctgggggc caatcacgat gagggccagg acgagaacga cgaggatttc
     4561 gagaaagccg acgccgaaga catcaaagtt tggcatgggg attcattcgg gcgttgccgg
     4621 ggaaagcaat cgccgcgccg gcggccatgg gcggcgcggc gcggcgacca accggattcg
     4681 cttgcgacct agctatcctc gattccgtgt tccttcaaat acgtaactgc atcctggacc
     4741 gtcgtgatct tctcggcctc ttcgtcggag atctcgagct ccaccccgtc cttggagaac
     4801 tcctcctcca tcgacatgat cagctcgacg aggtcgagcg aatcagcatt caagtcgtcg
     4861 acgaacgacg tagtcggcgt cacctggtcc tcgtccacac cgagctgttc cacgataatc
     4921 ttctggactc gctcgaagac ggttgccaac gctgactccc ttcattcgtt gtacatcctc
     4981 cgcggcacga gcgccgcgcg atggccctct ggatatgtta ccgcgactgg cccgtcgcgg
     5041 acccggcaat ggtacgaagg acgccgttca caaagcgtcc cgagctctca cttccgaatc
     5101 tcttggcaag tgccacagcc tcgttcagca cggcggctac cggcgcccta ttatcaaata
     5161 cgacctcgta gattgcaagt cgcaacacgt tccggtcgat ggccgcgatg tcctccacgg
     5221 ggcgctgcgg cgctgcttcc gccaacagcc ggtcgatctc ggagcggtgc atattcacgc
     5281 cgcggaggag cgattctgcg agctcgcggg ttggggcgtt cacctcctcc gcttccagca
     5341 tgcgcgccag tgccgcgtcc gccgaatggc ctgtagtgtc cgattcgtag agcgcctgca
     5401 aggcgagggt gcgagcttgc cttcgtgccg gacctgccat cgaacgtccc caggctagag
     5461 tgagatgccg ccatcaacgg cgagcacttg tcccgtaatg taggatgccg catcggatgc
     5521 caggaaggct accagctcgg cgacatcatc gactgtgcca aagcgccgga tggccatgac
     5581 gctgagagcc tgctcgcgca gatcgcgcga gaggccagta gtcatctccg tatcgatgaa
     5641 accgggagca acggaattcg ccgtgatgcc gcgcgagccc atctccttgg cgactgagcg
     5701 gatgaacccg ttgacgccgg ccttcgcggc ggcgtagttg gcttggcccg cgttgccgat
     5761 gatgcccgcg acgcttgaga tggcgatgat ccggccccag cggcgacgca tcatgccacg
     5821 gagcactgcc ttcgtgcaga ggaaggttcc gcgaaggttc gtatcgatga ccgtgtccca
     5881 gtcatcctcc gacatgcgga gcaagaggtc atcatgcgtg aggcccgcat tggcgatgag
     5941 gatgtcggtg acgccgagtc gtgcctccgc ttcacggacg atgcgctcca catctgttcg
     6001 gactgcgaca tcgccgccaa ttgctatggc acgtccgccg gcgctcacga ttgcgtcgac
     6061 aacctcgcgt gctgcatctt ctcgagtctt gtagttcacc gcgacggccg cgccgcgctg
     6121 ggcaagcaac aacgataccg cgcgaccgat gccgcgcgat ccccccgtga cgagagcgac
     6181 gtgtcccgcc aggctcagag gaccgctcac ggcgatgctc cggcaatcga agccatgt
//
LOCUS       DATMBB010000267         5178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_31932, whole genome shotgun sequence.
ACCESSION   DATMBB010000267 DATMBB010000000
VERSION     DATMBB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31932"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VNL92_05745"
     CDS             complement(<1..608)
                     /locus_tag="VNL92_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWO94248.1"
                     /translation="MLDAVRSDIFRQRKRLMPTVLLVIFALIACALYVVPWIAEKAQG
                     DEMNLRDLLDLRASLWIQNVIPYGLQFQSFFGLVLTAVLAGSSVGGEFSSGTVRTIII
                     RTRARWHFVAAKLTTIALFALLLPTLGMTLHLALAAAMTRDVGGNFDDVFTFAYAGDL
                     ATAVLRAFWCLLTYATLVVLLGVWTRSTAVAIAGSIGALFLEP"
     gene            complement(601..1575)
                     /locus_tag="VNL92_05750"
     CDS             complement(601..1575)
                     /locus_tag="VNL92_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94249.1"
                     /translation="MSTTTLRVTPNERSPNSEHDVVLRVRGLVKRYGAITAVRDLDLD
                     LYRGEVFGLLGPNGSGKSTTLGCVLGLIAPNHGRIEAFGFDLTRDRWEVLRRIGAIIE
                     TPALYGHLSGRDNLQALAISVGGLPQGRIEELLDLVGLRERADDKFKTYSLGMKQRLG
                     IASTLLTDPELIILDEPTNGLDPAGQREVRNLIPRLAAEGRTVLLASHLLHDVEQVCD
                     RIAILRRGEVLAVGGVGELLRTERRFRISLSPAELAPAETILRSMAGIAAIEQRDTSL
                     VVNAPSLTGRDVNRALSEAGIFAEELRSEGASLEDYFLELTTEETADA"
     gene            complement(1627..2835)
                     /locus_tag="VNL92_05755"
     CDS             complement(1627..2835)
                     /locus_tag="VNL92_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO94250.1"
                     /translation="MTEPGRVAPLVRVPRTVLLLGLVSLFADISSEMVYPLLPLFLTE
                     TLGAPATAVGLIEGIAEGTASVLKSVSGRLSDVMSRRKPFVVAGYALSAAAKPLLAAA
                     FVWPAVLLVRFVDRTGKGLRTAPRDAILAEATRERTRGRAFGLHRALDTAGAVLGPLA
                     ALGLIAILGDRYRVVFLVAFLPGLVSVALLSGVREASSARNATNSEAEGGALGWRGLG
                     RPYYILLGATLLFAIGNSSDAFLLLRADDLGLGATSVVLAYVTFNTTFAFLATPAGSL
                     SDVLGRRNVLVAGYVIFAVVYVAFGLVESKQAVWLLFPLYGLFMAMTDGVGRAYVADF
                     APRDRLGAALGLYHAVVGVAALLASLIAGALWDAIDPAAPFFLGGATAALTALLLLVA
                     LPSARPADPI"
     gene            2981..3466
                     /locus_tag="VNL92_05760"
     CDS             2981..3466
                     /locus_tag="VNL92_05760"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWO94251.1"
                     /translation="MPTKDEIVNALDGVVARVQELAPKVREKPNAKLPEGDWVVRDAL
                     CHLAARSNSVPMVIGLAERVAQSGGGGFPAGFDIDAVNQEQIEERRARTVDELINEII
                     EGHAVAKAEVRGVDDGLFERRLPNPRGEGELALADFLFAATAWHDNMHLDDIAKAVDG
                     A"
     gene            complement(3488..4399)
                     /locus_tag="VNL92_05765"
     CDS             complement(3488..4399)
                     /locus_tag="VNL92_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWO94252.1"
                     /translation="MTTTERIGIITDSVAVLPQHIRDELHIHVIPLNLEIDGRSYKDG
                     IDLEPDDFYTLMRSSDRPPTTSQPSIGDFLEIYARASEGVDRTVYFALSSRLSRTCET
                     ARLAARQFEEETGRHVDVFDCLTAASSQGLIVSEAARVARAGRGYEAMLRRAQELRPK
                     VVMYAVVETLEYLVRGGRVPRVAGWVGEALHVRPMIKLKDGLVSRAGFARSMKHGFQR
                     MGSHVQREIDAVRAAFGQAELRVAVMHADNRAGGEAFLHVVNQRFGPTESYLTAMTPV
                     MGAHTGPGLVGLGYFVEAPVERAKYYD"
     gene            complement(4419..>5178)
                     /locus_tag="VNL92_05770"
     CDS             complement(4419..>5178)
                     /locus_tag="VNL92_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO94253.1"
                     /translation="NGERLRAAADEIRTIAPDAEVEPIVVDLSSLAETRRLAEQVRAN
                     HDRLHVLINNAGVIRMDRRTTVDGLEETFVINHLSHFLLSSQLLDLLRSSAPARIMNV
                     SSATHKGATLDFDDLQGERSYGGYLAYRRSKLANILFTYELARRLEGTGVTVNCLHPG
                     AVATRFASGNRGVLGRLLRFGYIVGKPVMRSAAKGAETSIYLATSPDVEGVTGQYFEN
                     CRPAESSRQSYDEEAARKLWAISEQLVGGDRKAP"
BASE COUNT          931 a         1647 c         1784 g          816 t
ORIGIN      
        1 ggctccagga acaacgcgcc gatcgagccc gcgattgcga cggccgtgga tcgagtccat
       61 acgccgagta acacgacgag cgtcgcatag gtgaggagac accagaatgc tcgcagcacg
      121 gcggtcgcga gatcgcccgc ataggcgaac gtaaacacat cgtcgaagtt cccgccgacg
      181 tcgcgcgtca tcgcggcggc aagcgccagg tggagtgtca tcccgagcgt cggaaggagc
      241 agggcgaaga gcgcgatcgt tgtgagcttg gcggcgacga aatgccaccg ggctcgcgtg
      301 cggatgatga tggtgcgtac ggtgccggag gagaactcgc ctcccacact gctgccggcc
      361 agcaccgccg tcaggacaag gccgaagaac gattggaatt gcaggccgta cggaatcaca
      421 ttctgaatcc aaagcgaggc gcgcagatcg agcaggtcgc gcaggttcat ctcgtcgcct
      481 tgtgccttct ccgctatcca cgggacgaca taaagcgcgc aggcgatgag cgcgaagatg
      541 acgaggagca ccgttggcat caggcgtttg cgctggcgaa agatgtcgct ccggacggca
      601 tcaagcatcg gccgtctcct ctgtcgtcag ctccaggaag tagtcctcga gcgacgcgcc
      661 ctcgctgcgc agctcttcgg cgaagatgcc tgcttccgag agggcgcggt tgacatcgcg
      721 gcccgtgagg ctcggcgcat tcacgacaag ggatgtatcc cgctgctcga tggcagcgat
      781 gccagccatc gagcgcagga ttgtctcggc gggagcgagc tcggctggcg agaggctgat
      841 gcgaaagcgc cgctccgtgc gcagcagctc gccgacgccg ccgaccgcga gcacctcccc
      901 acgtcgcagg atggcgatgc ggtcgcagac ctgctccacg tcgtgcagca gatggctggc
      961 cagaagcacg gtgcgcccct cggccgccaa gcgggggatg agattgcgca cctcccgctg
     1021 gccggccggg tccagcccgt tcgttggctc atcgagaatg atgagctccg ggtcggtgag
     1081 cagcgtggag gcgatgccca gccgctgctt catcccaagg gagtacgtct tgaacttgtc
     1141 atcggcgcgc tcccgaaggc ccacgagatc gagcagctcc tcgattcgtc cctgcgggag
     1201 tccgccgacg gagattgcga gcgcttggag attgtcacgc cccgagagat gaccatagag
     1261 ggcgggcgtt tcgatgatgg cgccgatgcg acgcagcacc tcccacctat cgcgcgtgag
     1321 gtcgaagccg aacgcctcga tgcggccgtg gttgggggcg atgagcccaa gaacgcaccc
     1381 gagcgttgtg gacttgccgg agccgtttgg tccgagcagg ccgaacacct cgccgcggta
     1441 caggtcgagg tcgaggtcgc gcaccgccgt gatggccccg tagcgcttca cgagaccacg
     1501 cacgcggaga acgacatcgt gctcggagtt gggggaccgc tcgttgggtg tgactcgtaa
     1561 cgtggtcgtt gacacggtcg atcctagggg ttggttcgcg gcgctttcac catagtaacc
     1621 gacgcatcat attgggtctg caggccgcgc cgaaggaagg gcaacgagca gaagaagggc
     1681 ggtgagggcg gccgtcgcgc ctcctaggaa gaacggcgcc gccggatcga tcgcgtccca
     1741 gagcgcgccg gcgatgaggc tggcgaggag cgcggccacg ccgactacgg cgtggtagag
     1801 gccaagcgca gctcccagac gatcacgcgg ggcgaagtcc gccacgtacg ctcgccccac
     1861 gccgtccgtc atggccatga acaacccgta cagtgggaac aggagccaga ccgcctgctt
     1921 cgactcgacg agcccgaagg cgacgtacac aacagcgaag atcacgtagc ccgccacaag
     1981 cacattgcga cgaccaagga cgtcggagag cgagccggcc ggcgtcgcca agaacgcgaa
     2041 cgtcgtgttg aacgtcacgt aggccaagac gacggaggtc gcacccaggc cgaggtcgtc
     2101 ggcacggagg aggaggaacg catcgctcga gttcccgatg gcgaagagca gtgtcgcgcc
     2161 tagcaggatg tagtacggtc gcccgaggcc acgccatccc agcgcgcctc cctcggcctc
     2221 gctgttcgtc gcattcctcg ccgacgaagc ctcccggacg ccgctcagga gagcgacgct
     2281 gacaagcccg ggaaggaagg cgacgaggaa gacgacccga tagcgatcgc ccagaattgc
     2341 gatgagcccg agcgcggcga ggggcccgag aacggctccg gcggtatcga gagcgcggtg
     2401 gaggccgaag gctctgcccc tggtccgctc gcgagtcgct tcggcgagga tggcgtcgcg
     2461 aggcgccgtg cgcaagcctt tcccagtacg gtcgacgaag cgcacgagca gaacggcggg
     2521 ccacacgaag gccgcggcga ggagaggctt tgcggcggcg ctcagggcat agcccgcgac
     2581 gacgaacggc ttccgccggc tcatcacgtc ggacaggcgg cccgagacgg acttgagcac
     2641 gctcgccgtg ccttcggcga tcccttcgat gagcccaacg gccgtggccg gcgcgccgag
     2701 cgtttcagtg aggaagagcg gcagcagcgg gtacaccatc tcgctgctga tatcggcgaa
     2761 gagcgagacg agacccagca gcaggacggt ccgcggaacg cggaccagcg gtgcgaccct
     2821 acctggttcc gtcacgagtc gagtatcgca cctcggcccg attgacggat cgatcgagcg
     2881 ttgcgttcaa taaggttcgg ccgcgcgcgc cctgtgctcg aagatcgccg cgggcctgcg
     2941 cgtcgtacga atgatggatg tgaaggaagg aggccgcgct atgccgacca aggacgagat
     3001 cgtcaacgcc ctcgatggag tcgtggctcg cgtgcaagaa ctggctccca aggtccggga
     3061 gaagccgaac gccaagctgc cggagggaga ttgggtcgtg cgcgatgcgc tctgccacct
     3121 ggccgcacgc tccaacagcg tccccatggt catcggcctg gcggagcgcg tggcgcagtc
     3181 gggcggaggg gggttccctg caggcttcga catcgatgcg gtgaaccagg agcagatcga
     3241 ggagcggcgg gcgcgcacgg tcgacgagct catcaacgag atcatcgagg gccacgccgt
     3301 cgccaaggcg gaggtgagag gcgtcgatga tggcctgttc gagcggcggc tgccgaaccc
     3361 gcgcggcgag ggcgagctgg cgctcgcgga cttcctgttc gctgcgaccg cctggcacga
     3421 caatatgcac ctcgacgata tcgccaaggc cgtcgatggg gcgtaggttc ttgcgtcgac
     3481 tcgacgttca gtcgtagtac ttggcgcgtt ccaccggcgc ttcgacgaag tagccgaggc
     3541 ccacgaggcc ggggcccgtg tgcgccccca tgaccggcgt cattgccgtc agatagctct
     3601 cggttgggcc gaagcgctgg tttacaacgt gaaggaaggc ctcaccgccg gctcgattgt
     3661 ccgcatgcat caccgcgaca cggagctcgg cttgtccgaa tgcggctcga acagcgtcga
     3721 tctcccgctg cacgtgcgag cccatgcgct gaaagccgtg cttcatgctg cgtgcgaagc
     3781 cggcgcgcga taccaggccg tccttgagct tgatcatggg cctcacgtgc agcgcctcgc
     3841 cgacccagcc ggcgacccgc gggacacggc cgccgcggac cagatactcg agtgtctcga
     3901 ccacggcgta catcacgact ttggggcgca gctcctgcgc acggcgcagc atcgcctcgt
     3961 agcctcgtcc cgcccgcgcc acccgcgccg cctcgctgac gatgagccct tgggaagatg
     4021 cggccgtcag gcaatcgaac acgtcgacgt gtcgcccggt ctcctcctcg aactgtcgcg
     4081 cggccaagcg ggcggtctcg caggtgcggc tgagccggct gctgagggcg aagtagaccg
     4141 tccggtccac gccttcggag gcgcgtgcat aaatctccag gaagtcgcct atgctgggct
     4201 gtgacgtggt cggaggccgg tctgacgatc gcatgagcgt gtagaagtcg tcgggctcca
     4261 aatcgatgcc gtccttgtag gagcgcccgt cgatctcgag gttcagcggg atgacgtgga
     4321 tgtgcaattc gtcgcgaatg tgctgcggca gcacggcgac actatccgtg atgatgccga
     4381 tcctctcggt cgtcgtcatt cgtttccccc ttcttccgct acggcgcctt ccggtcgccc
     4441 ccgaccagtt gctcgctgat ggcccacagc ttgcgcgcgg cctcctcatc gtacgactgc
     4501 cgtgaggatt ctgcgggtcg gcagttctcg aagtactggc cggtcacgcc ctccacgtcg
     4561 ggcgacgtcg ccaggtagat gctcgtctcg gcgcccttcg ccgcgcttcg catgaccggc
     4621 ttgccgacga tatagccgaa ccgcaagagg cggccgagca ctcctcgatt gccgctcgcg
     4681 aagcgcgtcg ccacggcgcc cgggtggagg cagttgaccg tgacgccggt cccctccaag
     4741 cggcgggcga gttcgtaggt gaacaggatg ttggccagct tcgaccgccg gtacgcgagg
     4801 tatccgccgt agcttcgctc gccctgcagg tcgtcgaagt ccaacgtcgc gcccttatgc
     4861 gtggccgacg agacgttcat gatgcgggct ggtgcggacg atcgcagcag atcgagcagc
     4921 tggctgctga gaaggaagtg ggagaggtgg ttgatgacga acgtctcctc gaggccatcg
     4981 acggtcgtcc ggcgatccat gcgaatgact ccggcgttgt tgatcagcac gtgcagccgg
     5041 tcgtggttcg cccgtacttg ctccgcgaga cggcgcgtct cggcgagaga ggaaaggtcg
     5101 acgacgattg gttcgacttc ggcgtcaggg gcgatggttc gaatctcgtc cgcggccgca
     5161 cgaagacgtt cgccgttg
//
LOCUS       DATMBB010000268         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_70276, whole genome shotgun sequence.
ACCESSION   DATMBB010000268 DATMBB010000000
VERSION     DATMBB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70276"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VNL92_05775"
     CDS             complement(<1..268)
                     /locus_tag="VNL92_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94254.1"
                     /translation="MSNRWARLDLGLVAVVVAGALSAAIYATLTVRMSLQEFFPGPGY
                     TIDFKDMLGPDWRENWWLLWSAFGALFLLWGVALSRVGGARDRTV"
     gene            complement(332..1618)
                     /gene="serS"
                     /locus_tag="VNL92_05780"
     CDS             complement(332..1618)
                     /gene="serS"
                     /locus_tag="VNL92_05780"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWO94255.1"
                     /translation="MLGLPYIREHADELRDILANKLFTDAPIDEILRLDDERRRLVSE
                     ADDLRARRNAAGKVIGRAKDPEERQRLIDAQRAESQRIDELEARLAELEPRLQQLLLE
                     VPNIPDPDVPVGRDDSSNIVVAMEGALPEFPFEPLPHWELGERLGVIDFERGVKLSGS
                     RFYVLRGAGARLQRALIDWMLDMHREEHGYTEVYPPFLVREETLVGTGNLPKFADNLY
                     RDIEDDLWLVPTAEVPVTNLHRDEILPSEALPLRYVAYTPCFRREKMSAGRDVRGIKR
                     GHQFDKVELVKLVHPEDSDDELRTLLADATHIVRGLGLAYRVVQLCTGDLGFKAAKAF
                     DVELWAPGCGEWLEVSSCSNFRDFQARRANIRFRRSREDRPEFVHTLNGSALALPRLM
                     IAILETYQREDGTVDVPPVLHPYMRGTTQLRPEPHV"
     gene            complement(1618..2100)
                     /locus_tag="VNL92_05785"
     CDS             complement(1618..2100)
                     /locus_tag="VNL92_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWO94256.1"
                     /translation="MERHFTVTGYVVHNDRTLLHWHRKLGMWLPPGGHIDPNEDPVSA
                     VIREVREETGLQVELLPQRPPVRGFGRPVQIAPPVTILLEDIDEPGRPHQHIDMIYFT
                     RVMVPSQPSVDAEAWRWATLRELEAARLDGAAAPAIAEDVRKLGLLAIARFAEATVAG
                     "
     gene            2197..>3252
                     /locus_tag="VNL92_05790"
     CDS             2197..>3252
                     /locus_tag="VNL92_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWO94257.1"
                     /translation="MVESSRQAPAIASVRAELPATQSCVYLNTGTYGPLPRMAVEAIE
                     QHERADLERGRARPESFQASNEASATLRGLFAQVVGARDDEIALTYGTSHGMDIALWG
                     LDWREGDELVTTNVEHIGGLAAPYILRDRFGVVLRFADCTDPGRAAEEIRASLSPRTR
                     AIVMSHVSYSTGEALPVAEVGRVARERDALLIVDGAQSAGATPVAVHDLEVDAYAIPG
                     QKWLCGPGGTGALFIAGRSRERFRLTFAGYASFADFDEHGDALPHPSARRFELGNVHT
                     PLVFGAQAALRWLLNDLGLPWIFERTQAMTQLARRRLAEIDGIHVTTSRNANSPLTHF
                     TVRGWEPAAVVEELSARG"
BASE COUNT          565 a         1094 c         1073 g          520 t
ORIGIN      
        1 ggacggtgcg gtccctcgcc ccgccgacac gactgagcgc gacaccccaa agcaagaaca
       61 aggccccgaa cgccgaccag agcagccacc agttctcccg ccagtcggga ccgagcatgt
      121 ccttgaagtc gatcgtgtag ccggggccgg ggaagaactc ctggagcgac atccgtacgg
      181 tcagcgtcgc gtagatggcc gccgatagtg cgccggcaac gaccacggcc acgagcccca
      241 ggtcgaggcg cgcccatctg ttcgacatgc ggacatccta ggtggccgga ggcggacgca
      301 caacggcgag gccgcctatg gcgtggccag gctagacgtg aggctcgggc cgcagctgcg
      361 tcgtaccgcg catgtagggg tgcagcacgg ggggaacgtc gacagtccca tcctcccgct
      421 ggtacgtctc gaggatcgcg atcatcagtc ggggcaaggc gagcgccgag ccattgagtg
      481 tatgcacgaa ctcgggccga tcctcgcggc tgcgacggaa gcggatgttg gcgcgccggg
      541 cctggaaatc gcggaaattg gagcaggagg aaacctcgag ccactcgcca catccgggcg
      601 cccacagctc gacgtcgaaa gccttcgcgg ccttgaaccc aagatcgccg gtgcaaagct
      661 gtaccacgcg gtaggccaag cccaaaccgc gtacgatgtg cgtggcatcg gcgagtaagg
      721 ttcgaagctc gtcgtccgag tcttccggat ggacgagctt aaccagctcc accttgtcga
      781 actggtggcc ccgcttgatg ccgcgaacgt cccgacccgc gctcatcttt tcgcgacgga
      841 agcagggtgt gtaggccacg tagcgaagcg gcaaggcctc cgacggcaga atctcgtccc
      901 ggtgcagatt cgtgaccggc acctcggccg tcgggacgag ccacaggtcg tcttcgatat
      961 cgcggtagag gttatcggcg aacttgggca gattgcccgt gcccaccaag gtctcctcgc
     1021 gcaccaagaa cggcggatag acctccgtgt agccgtgctc ctcgcgatgc atatcgagca
     1081 tccaatcgat gagcgcacgc tgcaaacgcg cgcccgcacc gcgcagcacg tagaagcgcg
     1141 agcccgagag cttgactccc cgctcgaagt cgatgacgcc cagtcgctcg cccagctccc
     1201 agtgcggcag cggctcgaac gggaactcgg gcagcgcacc ctccatcgcc acgacgatgt
     1261 tggaggaatc gtcccggccc acgggaacat ccgggtcggg gatgttcgga acctcgagga
     1321 ggagctgctg gaggcggggc tcgagctcgg ccagcctcgc ctcgagttca tcgatgcgct
     1381 gcgattcggc ccgctgcgcg tcgatgagac gctgccgctc ctctgggtct ttcgcccgcc
     1441 cgatgacctt accggcagcg ttgcggcgtg cgcgcaggtc gtctgcttcc gatacgaggc
     1501 gccgtcgctc gtcatcgaga cggagaatct cgtcgatcgg cgcgtccgtg aagagcttgt
     1561 tcgcgaggat gtcgcgcaac tcgtcggcat gctcccggat gtatggcagt cctaacatca
     1621 gcccgcgacc gtcgcttcgg cgaatcgcgc tatggccagc agacccagct tgcgcacgtc
     1681 ctcggcgatg gctggcgccg ctgcgccatc caatcgcgcc gcctccagct cccgtaacgt
     1741 agcccagcgc caggcctccg catcgacgga cggctgcgac gggaccatga cccgcgtgaa
     1801 gtagatcatg tcgatgtgct ggtgcggcct tcccggctcg tcgatgtcct cgaggagaat
     1861 cgtgacgggc ggagcaatct ggaccggccg cccgaaccct cgaacgggcg gcctttgcgg
     1921 cagcagctcc acttgcagac cagtctcctc gcgcacctcg cgaatcacgg cactcaccgg
     1981 atcctcgttc ggatcgatgt gcccgccggg cgggagccac atgcctagct ttcgatgcca
     2041 gtgcaggagt gtcctatcgt tatggacaac gtagccggtg acggtgaaat gacgctccac
     2101 ccggccagta tagcgggacg agcgctgatg ggccctcgac gtgcgccaga tgcggcggcg
     2161 tggagtacgc tagcaaccgg aaggaggaaa gacgagatgg tggaatcgag ccgccaggcg
     2221 ccggcaatcg cgagcgttag ggcggagctt ccggcgacgc agagctgtgt ctatctcaac
     2281 accgggacgt acgggccttt gccgcgcatg gccgtcgagg ccatcgagca gcacgagcga
     2341 gccgacctgg agcgaggacg ggcgcgaccc gagagctttc aggcgagcaa cgaagcatcg
     2401 gcgacgctcc gggggctctt cgcacaggtg gtcggcgcgc gagacgatga gatcgccctc
     2461 acgtacggca cctcccacgg aatggacatc gccctttggg gcctcgactg gcgcgagggc
     2521 gacgagctcg tcaccaccaa cgtcgagcac atcggaggac tggcggctcc ctacatcctg
     2581 cgcgaccgct tcggcgtcgt gcttcgattt gccgattgca cggatcccgg acgcgctgct
     2641 gaagagatcc gcgcctccct ttcgcctcgc acccgcgcga tcgtcatgtc ccatgtctcg
     2701 tattccaccg gcgaggccct ccccgtggcc gaggtcgggc gcgtcgcacg ggagcgggac
     2761 gcgctgttga ttgtcgacgg cgcccagtcg gcgggagcga ccccggtcgc agtgcacgat
     2821 ctcgaggtcg atgcttacgc catccctgga cagaagtggc tctgcggccc cgggggcacc
     2881 ggcgccctgt tcatcgccgg ccgctcgcgc gagcgctttc ggttgacgtt cgcggggtat
     2941 gcctcgttcg ccgatttcga tgagcacggc gatgcacttc ctcaccccag cgcacgccgg
     3001 ttcgagttgg gcaacgtcca cacaccgttg gtgttcggcg cgcaggccgc gcttcgctgg
     3061 ctcctgaacg acctcggcct tccctggatc ttcgagcgga cgcaggcgat gacgcaactc
     3121 gcgcgccgcc gcctcgccga gatcgacggc atacacgtca cgacgagccg taacgcgaac
     3181 tcgccgctca cgcacttcac ggtgcgaggc tgggagcccg cggctgtcgt ggaggagctc
     3241 tccgcgcggg gc
//
LOCUS       DATMBB010000269         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_256181, whole genome shotgun sequence.
ACCESSION   DATMBB010000269 DATMBB010000000
VERSION     DATMBB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256181"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VNL92_05795"
     CDS             complement(<1..1207)
                     /locus_tag="VNL92_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94258.1"
                     /translation="MWDLLIRHTIRPDGEDGFLLPYHEYLEQTGDAAEDARRERLLRE
                     IAVPADPAHVRVFSYAAELAPADIALSTLVRCLESVRKIREHGIAKGPWERREEWLNQ
                     QIALAWQDRGPFPGLGPALEALGMRLGTALALELRSSGMVKADADPWPTVDAILRGKQ
                     KPPQPAYAEDLKAIRETWSSLPDERRALLKLLSRFALTSAQALRWFDPKERAKGTPGK
                     VSDEEILANPYRMSEVDLGDWNDSPVSVGLIDRGLLPDATIAAKQPVPAPSKVGSPND
                     ARRLRAALVAVLREASENGDALLSVPEALQRVGSLDLVHPCVIGSDWPSTNRTTLSGV
                     IELVEVAGTGATPTSALQLTELKGREDRLRSVLGKRVGKPAAPVKADWQKLLVAAISE
                     AGGKFDKTNE"
     gene            complement(1107..1847)
                     /locus_tag="VNL92_05800"
     CDS             complement(1107..1847)
                     /locus_tag="VNL92_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94259.1"
                     /translation="MFHLTVRVAWHDSRWNGTVCRKPSCNSFCVALDRIRLERNDARE
                     DALAGKQWSTLDPDDLPACKAESGAFMNEEEWTRRFVHPYAGIKKAEETHGHLKPTLV
                     RVPSYATFVVPFAWMLRSEQEAIDERLPTPLPPDEESPFASPWVFGRERQEAILKLFS
                     SRLTPERSLVFFYCKEGQPLGDTISRLVMGVGRIATLAPPKAYEVTKKKPHAPHVGPA
                     HSAHDPTGRRGRVPAPVPRVPRADGRRR"
BASE COUNT          325 a          674 c          686 g          373 t
ORIGIN      
        1 gctcgttcgt tttgtcgaac ttgccgccag cctccgagat cgcggcaacg agcagcttct
       61 gccagtcggc cttcacggga gcagcgggct tgccgacgcg ctttcccagg accgagcgaa
      121 gtcgatcctc acggcctttc agttctgtga gctgcagcgc ggaagtcgga gtagcgccgg
      181 tgccggcaac ttccacgagt tcgatgacgc cagagagcgt cgtgcgattc gtactcggcc
      241 agtccgagcc gatcacacaa gggtgaacga ggtccagcga gccgacgcgc tgcagcgctt
      301 ccggaacgct caggagtgcg tcaccgttct ccgaggcttc acgcagcacc gcaaccaacg
      361 cggcgcgaag acgtcgcgcg tcgttcggcg agcccacctt cgagggcgcc gggaccggct
      421 gcttcgctgc gatggtcgcg tcagggagca agccgcgatc gatgagcccc acggataccg
      481 gcgagtcgtt ccagtcgccg aggtccacct cgctcatccg atacgggttc gcgaggatct
      541 cctcgtcgct caccttcccg ggcgtgccct tggctctctc cttcgggtcg aaccagcgaa
      601 gcgcctgcgc cgacgtaagc gcgaatcgcg acagcagctt gagcagagcg cggcgctcgt
      661 cagggaggct cgaccacgtc tctcggatcg ccttcaggtc ctccgcatag gccggctgcg
      721 gcggcttctg tttccctcgg aggatcgcgt ccacggtcgg ccacgggtcg gcgtcggctt
      781 tgaccattcc cgacgaccgc agctccagcg cgagggccgt cccgagtcgc atcccgagcg
      841 cctcgagcgc ggggccgagc ccagggaacg gtcctcggtc ttgccatgcc agtgctatct
      901 gctggttgag ccactcctcg cggcgctccc acggcccctt cgcgatcccg tgctctcgaa
      961 tcttgcgcac cgattcgagg caacggacca gcgtcgacag cgcgatgtcg gcgggcgcca
     1021 gctctgcggc gtaggagaag acgcgaacgt gtgccgggtc ggcgggcacc gcgatctccc
     1081 gcaggaggcg ttcacgccga gcgtcttcag cggcgtcgcc cgtctgctcg aggtactcgt
     1141 ggtacgggag caggaacccg tcctctccgt ccggtcggat cgtgtgccga atgagcaggt
     1201 cccacatgag gtgcgtgtgg cttcttcttc gtgacctcgt aggccttcgg cggggcgagt
     1261 gtggcgatcc ttccgacgcc catcacgaga cgcgagatgg tgtcgccgag cggctggccc
     1321 tccttacagt agaagaagac caatgagcgc tccggcgtga gcctgctgga gaacagcttg
     1381 aggatcgcct cctgccgctc gcgaccgaag acccaaggac tcgcgaacgg ggactcctcg
     1441 tcgggcggaa gcggtgtcgg aagccgttcg tcgatcgcct cctgctcgct acggagcatc
     1501 cacgcgaacg gcaccacgaa cgtggcgtag gacgggaccc tgaccagcgt cggcttgagg
     1561 tggccgtgcg tctcctcggc cttcttgatg ccggcgtagg ggtggacgaa gcgtcgcgtc
     1621 cactcctctt cattcatgaa cgcccctgac tcggccttgc atgcgggcag gtcgtccggg
     1681 tccagagtgc tccattgctt ccctgccagc gcgtcctcgc gggcgtcgtt gcgctccagg
     1741 cggatgcggt cgagagccac gcagaaggag ttgcaggaag gcttgcggca gacggtgccg
     1801 ttccaacggg aatcgtgcca agcaacacgt acagttaggt ggaacatggg ctgttcagtc
     1861 ttcctgggtc tgacagtgat tacacgggct catggggctg agattcatga tgctgtcccc
     1921 cgcccgagga atgacgagcc gcgcgccagg cgagccttca ggccgtcagc gatgaagtcg
     1981 gcgagttttt cagcttcctt ccacttggtc gtgcggcggg tggggtcgaa gtcgtgggtt
     2041 cgaaccaggg cgcgtagc
//
LOCUS       DATMBB010000270         4222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_440537, whole genome shotgun sequence.
ACCESSION   DATMBB010000270 DATMBB010000000
VERSION     DATMBB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440537"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VNL92_05805"
     CDS             complement(<1..263)
                     /locus_tag="VNL92_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWO94260.1"
                     /translation="MSIREAIERAVGGGDLAEDEAAAVMEEIMTGAVTDAQLAAFLVA
                     LRMKGETVAEMAGMARVMREKAVPVMVEGPLLDTCGTGGDGSCS"
     gene            complement(288..875)
                     /locus_tag="VNL92_05810"
     CDS             complement(288..875)
                     /locus_tag="VNL92_05810"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWO94261.1"
                     /translation="MLLLIDNYDSFTYNLYQYLAELGEEMLVVRNDMITLDEVEALAP
                     ERIVISPGPCTPAEAGISTETIRRFAGRVPILGVCLGHQCIAEAFGGRVGAAGEIMHG
                     KMSLIEHDGEGVFRGLPNPFRAVRYHSLAADPISLPDELVVTARSESGVIMGVRHRRF
                     AVEGVQFHPESIMTEVGKDLLRNFLAIEEKAAVRP"
     gene            complement(912..1364)
                     /locus_tag="VNL92_05815"
     CDS             complement(912..1364)
                     /locus_tag="VNL92_05815"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWO94262.1"
                     /translation="MGTDVASTIVTISLLLVREGRALVVRRHGGHSESPGAWDLPGGR
                     LLDWEDDIDAAVLRIAQEELGVRSTGYEFFATQYAPLVGGEPRVDNLYIVDTWEGDPR
                     INSIDQHDAMKWAQLEELAHLPLVEPRRTLVQRLFGGTGDSLGESGWF"
     gene            complement(1354..2823)
                     /gene="trpE"
                     /locus_tag="VNL92_05820"
     CDS             complement(1354..2823)
                     /gene="trpE"
                     /locus_tag="VNL92_05820"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HWO94263.1"
                     /translation="MTYLPSLSEARRLAHSGAGNLLPVYREVRADLETPVSAYLKVAR
                     GRYSFLLESVEGGERVARYSFIGTEPYRVFSADGSEDPLVPVQEELERFRVLHPPALP
                     RFTGGAVGYLAYEAVRHFEPRVPCAPNDPLGLPEALFMFVDTLLVFDHLERNIKIVSH
                     MRLDGDVEAAYRSATQRIDELVERLSGPVPTSAAPRATPASGNVRSNVGKEAYLEFVE
                     SIREYIVAGDVIQVVPSQRLSRATSAAPFEIYRALRGINPSPYMYYLDLGDFQVAGAS
                     PEMLVRVEDGLVETHPIAGTRPRGRTVEEDDALAEELAHDEKELAEHVMLLDLGRNDV
                     GRVSEPGSVNVTTMMGVERYSHVMHLVSHVTGRLRSDLTAFDALRACFPAGTVSGAPK
                     VRAMQIIAELEPDRRGLYAGAVGYVDFSGNLDTAIAIRTMVMKDGVAHVQAGGGIVYD
                     SVGEAEHQESMNKAMALLRAIERAESMAESPQPAGVRGY"
     misc_binding    complement(2872..3147)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3559..>4222
                     /locus_tag="VNL92_05825"
     CDS             3559..>4222
                     /locus_tag="VNL92_05825"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWO94264.1"
                     /translation="MPENQHNHDALDTMRHSAAHVMAKAVQRLYPGARLAIGPTIEHG
                     FYYDIDVPETISEDDLPRIEDEMRRIVAAGEPFERIEMTRDDAIRHYREVEPNPLKVE
                     VLEGIDDPKVSFYKTGPDWLDLCRGPHVRSTREIGAFKLTSVAGAYWRGDERNPMLQR
                     IYGTAWPTEEELQEYLHRIEEAQRRDHRRLGRELDLYLTHELVGQGLPMLTPKGAVVR
                     RLL"
BASE COUNT          766 a         1475 c         1276 g          705 t
ORIGIN      
        1 gagcagctcc catcgccacc cgttccacac gtatcgagca ggggaccttc caccatcacc
       61 ggcacggcct tttcgcgcat cacgcgagcc atgccggcca tttccgccac cgtttcgcct
      121 ttcatccgca gcgccacgag gaacgccgcc agctgggcat cggtcacggc gccggtcatg
      181 atctcttcca tgacggcggc agcctcatcc tccgcgaggt ccccaccgcc caccgcacgc
      241 tcgatcgctt cgcgaatgct catgccccac tcccccgaaa tcagatgtta cgggcggacc
      301 gccgccttct cctcgatggc cagaaagttg cgcagcaggt cctttccgac ctcggtcatt
      361 atgctctcgg ggtggaactg gacaccctcc acggcgaagc gccggtgtcg cacgcccatg
      421 atgacgccgc tctccgagcg agccgtcacc acaagctcat cgggaaggct gatgggatcg
      481 gccgccaagg aatggtaccg aacggcacgg aacgggttcg gcaggccgcg gaacacgccc
      541 tccccgtcat gttcgatcag tgacattttg ccgtgcatga tctcgccggc cgccccaact
      601 cgccccccaa acgcctcggc gatgcattgg tgtccaaggc agacgcccag gatcggcaca
      661 cgacccgcga agcgtcgaat cgtctccgtg ctgatgcccg cctccgccgg cgtgcacggt
      721 ccgggcgata tcacgattcg ttcaggagcc agcgcttcca cttcatccag cgtgatcatg
      781 tcgttgcgga cgacgagcat ctcctcgcca agctcagcca gatactggta caaattgtag
      841 gtaaagctat cgtagttatc gatcagcagc agcacggctc tcccctcctc ctcgacacat
      901 tccctgcggc gctagaacca gccgctctcg cccaaagaat cgccggtccc gccgaacaaa
      961 cgttgcacaa gcgtgcgtcg cggctcgacc agcggcagat gcgccagctc ctcgagctgc
     1021 gcccacttca tggcgtcatg ctgatcgatc gaattgatgc gggggtctcc ctcccaagtg
     1081 tcgacgatgt acaggttgtc gaccctcggt tcgccgccga cgagcggcgc gtattgcgta
     1141 gcaaagaact cgtaccccgt cgaccgcacg ccaagctcct cctgagcgat gcgaagcacc
     1201 gccgcatcga tatcgtcctc ccaatcgagc agccggccgc ccggcagatc ccacgcgccc
     1261 gggctctcgc tgtgtccccc gtggcgtcgc acgaccaggg cgcgcccttc gcgcacaagg
     1321 agcaggctga tcgtaacaat cgtgctcgcc acgtcagtac ccacgtacac ctgccggctg
     1381 gggcgactcc gccatcgatt ccgcgcgctc gatggcgcgc agaagggcca tcgccttgtt
     1441 catgctctcc tggtgctccg cctcaccgac gctgtcgtag acgatgccac ccccagcctg
     1501 tacgtgcgca accccatcct tcatgaccat cgtgcgtatc gcgatcgctg tatcgagatt
     1561 tccggagaaa tctacgtagc ccacggcgcc ggcgtacagg ccgcgccggt ccggctcgag
     1621 ctcggcgatg atctgcatcg cgcgtacctt cggcgcgccg gagacggtgc ccgcgggaaa
     1681 gcatgctcgc agcgcgtcga aagcggtgag atcgctgcgc aggcggccgg tcacgtgcga
     1741 cacaaggtgc atcacgtgcg aataccgctc gacccccatc atcgtcgtca cgttcacgct
     1801 gcccggctcg ctgacgcggc ccacgtcgtt ccgcccgagg tccaggagca tcacgtgctc
     1861 ggccagctcc ttctcgtcat gcgccagctc ttccgcgagc gcgtcgtcct cctccacggt
     1921 ccggcctcgg ggacgggtac cggcaatggg atgcgtctcg acgagtccgt cctccacgcg
     1981 caccagcatc tctggggatg cgccggctac ttgaaagtcg ccgaggtcga ggtagtacat
     2041 gtacggcgag gggttgattc cccgcagcgc ccgatagatc tcgaacggcg cggccgacgt
     2101 cgcacgcgat agccgctgcg aaggaaccac ctgaatcaca tcgcccgcca cgatgtattc
     2161 gcggatcgac tccacgaact ccaggtacgc ttccttgcct acattcgaac gaacgttgcc
     2221 gctcgcgggg gtcgcgcgcg gcgccgccga agtcggcacg ggcccggata gccgctcgac
     2281 gagctcatcg atgcgttgag tcgcgctgcg ataggccgct tcgacgtcgc catcgaggcg
     2341 catgtggctc acgatcttga tgttgcgttc gaggtggtcg aacacgagca gcgtatcgac
     2401 gaacatgaac agcgcttcgg gcaggcccag cggatcgttc ggggcgcacg gcacgcgcgg
     2461 ctcgaagtgc cgcacagcct cgtaagcgag atagccgacg gcgccgcccg tgaaccgtgg
     2521 cagggcgggc gggtgcagaa cgcgaaagcg ctccaactcc tcctggacgg ggacgagcgg
     2581 gtcctccgag ccatcggcgg agaagacacg gtacggctcc gtaccgataa agctgtagcg
     2641 cgccacgcgc tcgccgccct cgacgctttc cagcaaaaag gagtatcggc cgcgcgctac
     2701 cttgagatag gccgaaaccg gcgtctcgag gtccgctcgc acctcgcgat acacgggcag
     2761 aaggttgccc gcaccgctgt gggcaagacg tcgcgcctcg ctcagcgatg gcagataggt
     2821 caacggtctt ccctcccctc gaaggagcca aaatacaacg atcccctcgc cgatagggac
     2881 gaggggatcg ctcgcggtac caccctagtt ggcctcctcg cggaaggccc actctcttcg
     2941 agacacggcc cgggcgcaca caagcggcag gcgatgctct agacttcgat aacggggtca
     3001 ccccggcggc gcctaccacc cgatgcaacg gggttcgggc cgcggctccc aggcccattt
     3061 caccgccgcg ccgtcgccgg gctcgcacct accccggctc tctgcaggct cgcgtttgcg
     3121 gctactcgtc ctgttcgctg ccgattcgct cttcagttat gtacgagcct agggcgaggc
     3181 gcgagctctg tcaagcggcg gaacaacacc gaacagtcct gttgacggtg tccatgtgtc
     3241 gtgccagtat ggggtgcgaa cacgtttata ctggcgacga ccaggacgag tagcgatgga
     3301 caccgcgtcc agagagcggg gccggagcct cgaagaggcc ggctgaaagc ccccgcccca
     3361 ggaccatcgc gaaaaccacc tgcagctggt gcggcgaagc gtttgtgctc agtagtcgcc
     3421 ccggatggcc ccgttaccgg ccgtaatgag gtcctctcgt gcctggtcgc gtggggatga
     3481 agcagggtgg aaccgcgagc cgcaagcctc tcgcccctac gggtgggagg cttgagtttt
     3541 ttcgcaggga gcgcggcgat gcccgaaaac caacataacc acgatgcgct cgatacgatg
     3601 cgtcacagtg cggcccatgt gatggcaaaa gccgtgcagc ggctctatcc gggcgcccga
     3661 ctcgccatcg gtccgaccat cgagcacggc ttctactacg acatcgatgt gcctgagacc
     3721 atcagcgagg acgacttgcc ccgcatcgag gacgaaatgc gccgcattgt cgccgctggc
     3781 gagcccttcg agcgcatcga gatgacgcga gacgacgcta tccgtcacta ccgcgaagtg
     3841 gagcccaacc cgctcaaggt cgaagttctc gaaggcatcg acgacccgaa ggtctccttc
     3901 tacaagaccg gtcccgactg gctcgacttg tgccgcgggc cgcacgtgcg ctcgacacgc
     3961 gagatcgggg cgttcaagct caccagcgtc gccggcgcct actggcgggg cgatgaacgc
     4021 aaccccatgc tgcagcgcat ctacgggacg gcctggccca cagaggagga gctccaggag
     4081 taccttcatc gcatcgagga ggcgcagcgg cgcgaccatc ggaggctcgg ccgcgagctc
     4141 gatctgtatt tgactcacga gcttgtcggc caaggcctcc cgatgctgac gccgaagggc
     4201 gccgtcgtcc ggcgactgct cg
//
LOCUS       DATMBB010000271         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_158680, whole genome shotgun sequence.
ACCESSION   DATMBB010000271 DATMBB010000000
VERSION     DATMBB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158680"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VNL92_05830"
     CDS             complement(<1..669)
                     /locus_tag="VNL92_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94265.1"
                     /translation="MPPLVVGTVALIVGVLASAASAEHTMLPLPAPAGTEWEIIAGYN
                     TATHSQADGNDPYAIDIVRVDGPTAGTPALAPVASTIGFVTDSCVGLEDDHGMTLLLC
                     HLFASPGLEGGSKVMPGQRIGTVAPDGAAGNNGIAHIHLAVSPTEGGASIPLVGQYAL
                     EGIALLPLAEPNAHAGERFVSTLLSSGIPVEPPSPEPEPAQPDAPIPEPAPVAPPAPP
                     VEPPP"
     gene            869..1954
                     /locus_tag="VNL92_05835"
     CDS             869..1954
                     /locus_tag="VNL92_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007974217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWO94266.1"
                     /translation="MKAAVFHGPNQPLTIENDVEMDKPMRREVVVRTVASGVCHSDLH
                     FIEGLYPGPAPGILGHESAGIVEAVGEDVTYVKPGDHVIVCLSVFCGHCEQCLTGHPN
                     RCRSDDVRRGRGEPPRYTWRGKPISQFANVGGYAEKMLVHENGIVKIDDEYPLDRAAL
                     IGCGVTTAVGAALNTAKVEPGSTVAVFGCGGVGLSAIQGAFIAGARQIIAVDMVEGKL
                     GMAREFGATHTVDASSRDPVQAIQEMTGGGVDYSFEAIGNKRAAEQAYESLRAGGTAT
                     IIGMIPVGQKVEIDGTSLLSERRLQGSGMGSNRFRVDMPKYIDFDRQGRLHLDKMITR
                     RGSIEDVNEAFRAMKAGEAARTVLMWS"
BASE COUNT          342 a          605 c          737 g          318 t
ORIGIN      
        1 cggcggcggt tcgaccggtg gcgctggggg tgcaactgga gctggctcgg gtataggcgc
       61 gtcaggctgc gcgggttctg gctcggggga cggaggctcg acggggatgc ctgagctcag
      121 gagggtcgat acgaatcgct cgccggcgtg cgcgttcggc tccgcgagcg gtaggagggc
      181 gatcccttcg agggcgtact ggcccacaag cgggatcgag gctccgccct cggtagggct
      241 cacggccaga tggatgtggg cgattccatt gttgccggcg gccccatctg gcgcgaccgt
      301 gccgatccgc tgtccgggca tgaccttcga gcccccttcg aggccgggcg atgcgaacag
      361 gtggcaaagg agaagcgtca tcccgtggtc gtcctcgagc ccgacgcagc tgtccgttac
      421 gaagccgatg gtggatgcca cgggagcgag ggccggtgtg cctgccgttg ggccgtcaac
      481 gcgaacgata tcaatggcgt acgggtcgtt gccatccgct tgggaatggg ttgccgtgtt
      541 gtacccggcg atgatctccc actcggtgcc ggccggggcg ggcaggggga gcatcgtgtg
      601 ctcggcgctt gccgccgagg ctagcacgcc aacgatgagg gccaccgtac cgacgacgag
      661 cggaggcacg caggggattc gtaatgatct gtgcataggt agatgttcgg cagaatgcca
      721 gcgtcggagc actcggtgcg ggccccacaa gagggccaga gtcgcccgta ggagccctcg
      781 tcgcggaatg tacttgacgt gcggcaggct cctaggtatg gtctcggcgg acccggaacg
      841 ggccggaagg ttgagggagg agacatttat gaaagccgcg gtgttccacg gccccaacca
      901 gcctctgacc atcgagaacg acgtcgagat ggacaagccg atgcgtcgag aggtcgttgt
      961 gcggacggtc gccagtgggg tgtgccatag cgacctccac ttcatcgagg ggctctaccc
     1021 ggggcccgcg cccggcatcc ttgggcacga gtcggccggc atcgtcgagg ccgtgggcga
     1081 ggacgtcacc tacgtcaaac caggcgacca cgtgatcgtc tgcctctcgg tcttctgcgg
     1141 gcattgcgag cagtgcctca ccggtcaccc gaaccgctgc cgtagcgatg acgtgcgtcg
     1201 tggacgcggc gaaccgccac ggtatacgtg gcgagggaag cccatctccc agttcgcgaa
     1261 cgtgggcggg tatgccgaga agatgctcgt gcacgagaac ggcatcgtaa agatcgacga
     1321 cgagtatccg ctcgatcgcg cagcgctcat cggctgcggc gtgaccacgg ctgtgggcgc
     1381 cgcgctcaac accgcgaagg tggaaccggg ctcgaccgtt gccgtattcg ggtgcggggg
     1441 cgtcggcctc tcagcgattc agggcgcgtt tatcgccggg gcccggcaga tcatcgccgt
     1501 cgacatggtg gagggcaagc tcggcatggc gcgcgagttc ggagcgaccc acacggtcga
     1561 cgcgtcgagc cgagatccag tgcaggccat ccaggagatg acgggcggcg gcgtggacta
     1621 ctccttcgag gcgattggca acaagcgcgc cgccgagcag gcctacgagt cgctacgggc
     1681 gggcggcacg gcgacgatca tcggcatgat cccggtgggc caaaaggtcg agatcgacgg
     1741 cacgagcctc ctcagcgaga ggcggctgca gggcagcggc atgggctcga accgcttccg
     1801 cgtcgacatg ccgaagtaca tcgacttcga ccggcagggg cggctgcatc tcgacaagat
     1861 gatcacgcgt cgtggctcga tcgaggacgt gaacgaggcc ttccgggcca tgaaggcggg
     1921 cgaggcagcc cgcaccgtcc tcatgtggag ctaggactgc caggataccg cgcttcgagg
     1981 gccgggcatt gcccggccct cg
//
LOCUS       DATMBB010000272         5609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_77503, whole genome shotgun sequence.
ACCESSION   DATMBB010000272 DATMBB010000000
VERSION     DATMBB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77503"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1000)
                     /locus_tag="VNL92_05840"
     CDS             complement(89..1000)
                     /locus_tag="VNL92_05840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO94267.1"
                     /translation="MAELSGGRVESLRRRAYLVERATDLATIRRWLETRRHYAAYALG
                     QFEPELTDLTEWWTATGDLGRGLVAFARGGLGDALFCMGEAEAVDAILRLHQGSRLTY
                     ATYQPEHLTVVRRRYMLQSPTVMSRMAVRGDTFRPSPPPRNGTLRRLYERDVQMLNRL
                     YGTEGGATYYSSSHLREGVYYGMLVDGRLVAAAGTHVVGPTQGIAVVGNVFTHPAHRG
                     RGFAKATTSAVTAALLERCSDVVLTVDPSNTPAVEAYRALGYEHVCEIMEGAAARKDP
                     IGLASFVRRRVAGWRGRKHGGEMVFAS"
     gene            complement(1011..1922)
                     /locus_tag="VNL92_05845"
     CDS             complement(1011..1922)
                     /locus_tag="VNL92_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWO94268.1"
                     /translation="MSILVAGTIALDNIETPFGSMASTLGGSATHFAAAASFYSRVNL
                     VSVIGPDLDQRELDFLRQRHVDMRGVQVVEGSTFRWAGRYDYDMNSRETLETQLNVFT
                     DFRPTIPDDYRRSQYIFLANMDPEQQRLVLEQVDQPQLTLMDTMDLWIQTRREELVRT
                     MRMVDMVTMNDSEVRMFGETISLVAAARQVLELGPRAVLIKKGEHGALLFTRTNEYFA
                     APAYPLEDVRDPTGAGDSFAGGFLGFIAECGQLNEDILKRAVIHGSAVASYTVEDYGV
                     RRMRTLTRSEIDRRYQEFRTFTHFEPI"
     gene            complement(1948..2826)
                     /gene="mtnP"
                     /locus_tag="VNL92_05850"
     CDS             complement(1948..2826)
                     /gene="mtnP"
                     /locus_tag="VNL92_05850"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWO94269.1"
                     /translation="MGGGSKDDVRFAVIGGTGVYDLESLAEAVRVEVATPFGMPSDAI
                     TIGSLDGVRIAFLPRHGGDHGIPPNELPSQANIWALKSLGVRQVLSISAVGSLRADIA
                     PQHIVVPHQLIDRTTGRPGSFFGRGLVAHISFAEPFCPRLSRAAGASAEAAGVTTHLG
                     GTCVVINGPRFSTRAESELYRSWGADVIGMTAAPEAQLAREAEMCYAALMAVTDYDTW
                     HEGHESVNANLIFQNLQKNAATTKTIVRLLVGSIPAPDGCACQNALADAFATPLARAS
                     DGKRRELAPIIGRYLK"
     gene            complement(2856..3368)
                     /locus_tag="VNL92_05855"
     CDS             complement(2856..3368)
                     /locus_tag="VNL92_05855"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWO94270.1"
                     /translation="MDLRAYVRDIPDFPQPGILFRDITPLLRTPEAMQYVLDTYEARY
                     RDAHLDAIVGIESRGFLFGAPLADRLRLPFVPIRKPGKLPAQRMSMEYALEYGTSQLD
                     IHADALDHGQRALIVDDLLATGGTAHAAAKLVELIGGVVAGCAFVIELTALGGRERLR
                     AYEAFSLLQY"
     gene            3481..3924
                     /locus_tag="VNL92_05860"
     CDS             3481..3924
                     /locus_tag="VNL92_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWO94271.1"
                     /translation="MKTALVQYLYDYQYAQLLTADGHAFVADEPTDSGGDGLGPDPYE
                     LLLWSLGACTAMTLLMYARRHDYPLADIAVQVEHDRVYPDDSAATAEPGAERRKIEVL
                     RRKITLRGDITDEQRERLLEIARRCPVHRTLAAAPEVVDTISVGE"
     gene            complement(3943..4140)
                     /locus_tag="VNL92_05865"
     CDS             complement(3943..4140)
                     /locus_tag="VNL92_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94272.1"
                     /translation="MNARSHVRVSHWFADSEERNERDVPAAELEAYLRSLRIMKSFSA
                     ALRVAVEDSEIDLGVQPKHVW"
     gene            4260..5120
                     /locus_tag="VNL92_05870"
     CDS             4260..5120
                     /locus_tag="VNL92_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94273.1"
                     /translation="MLISLETNGTGPSFGYTQQKVHQMEPRDEGAAREESRSPFARVS
                     EELARARREADRVLRDAERRAEEILHEAEEPRGAPVERSLAELLHQLGVQVTQVVASA
                     EEDRRLLREQMQLIREMLGAMREDIASLRSQAAPRVVAPPPVAPPPPMTEPPVAEAPA
                     AAPTPPPPAFEPPPPAFEPAPPEPSAPLVPDLEGTVFEARGQYVHLFIENVRTFGQLT
                     SIYQALLAMDSIQAISISRYQSDEAHLLLDLKVDVAAGSVVETLRAAGYDASIVEADA
                     GLLTLRLALS"
     gene            5165..>5609
                     /locus_tag="VNL92_05875"
     CDS             5165..>5609
                     /locus_tag="VNL92_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO94274.1"
                     /translation="MYLRHLTLNHYRTYARLEIELEPGVSVFIGENARGKTNLLEAIY
                     LLAVGRVARAELDGEAIAWDASDRPPFARVAGDVVVARGEMRAEAVIAAHQGWEEEPS
                     PSRPPRASKRFRINGVPRRLADFAGTFAATLFSVEDLAVVNGPPSG"
BASE COUNT          916 a         1856 c         1895 g          942 t
ORIGIN      
        1 ctccagagcg cggggcgaaa gtgacatcgc ctcgctgatg agaaccttcg aaccgtcctt
       61 tggatcatcg gcaggctctc gccccgtctc aggaggcgaa gaccatctcg ccgccgtgct
      121 ttcgtccccg ccagcctgcc actcggcggc gaacgaaact tgcaaggccg atgggatcct
      181 tgcgggcggc ggctccctcc atgatctcgc agacgtgctc gtagccgagc gctcgatatg
      241 cctcgaccgc cggcgtgttg ctcgggtcga ctgtgagcac cacatcgctg cagcgctcca
      301 gcagggccgc ggtcaccgcg gatgtggttg ccttcgcgaa gcctctgccg cgatgggcgg
      361 ggtgcgtaaa cacgttccca acgacggcga ttccctgcgt aggccccaca acatgtgtgc
      421 cggcggctgc aacgaggcgc ccatcgacga gcatgccgta gtatacgcct tcacgaaggt
      481 gtgaggaact atagtacgtg gcgccacctt cggtgccgta gagccggttg agcatctgca
      541 cgtcgcgctc gtagagcctc cgcagggtac cgttccgagg tggaggagat ggccggaacg
      601 tgtctccgcg cacagccata cgcgacatga ccgttgggga ttgcagcatg tatcgccggc
      661 ggaccaccgt cagatgctcg ggctggtacg tggcgtaggt gaggcgcgag ccttggtgga
      721 gccgcaggat ggcatcgacg gcctccgcct cgcccatgca gaacagcgcg tcacccaggc
      781 cgcccctcgc aaaggcgacg agcccgcgcc cgaggtcgcc cgtcgcggtc caccattccg
      841 tcaggtcggt gagctcaggc tcgaactgtc cgagagcgta ggcggcatag tggcgacgtg
      901 tctccagcca ccgccggatg gtggcgaggt ccgtcgcccg ctcgacgagg taggcgcgcc
      961 ggcgtaggga ctccacgcgc ccgccactca gctcggccaa ggatttcccc ctaaatcggc
     1021 tcgaagtgcg tgaacgtgcg gaactcctgg tagcgacggt cgatttcact tcgagtcagc
     1081 gtgcgcatgc gccgtacgcc gtaatcctcg acggtgtagc tcgcgactgc gcttccgtga
     1141 atgacggctc gcttcaggat gtcctcgttg agctgtccgc actcggcgat gaagccgagg
     1201 aatccgcccg cgaaggagtc gccggcgccc gtcggatcgc gcacgtcttc gagcggatac
     1261 gcgggtgcgg cgaagtactc gttggtgcgc gtaaacagca gcgctccatg ctcgcccttc
     1321 ttgatgagca cggcgcgcgg gccgagctcc agaacctgcc gcgccgccgc gacgaggctt
     1381 atcgtctcgc cgaacatgcg cacctcggag tcgttcatcg tgaccatgtc gaccatgcgc
     1441 atcgttcgga ccagctcctc acgccgcgtt tggatccaga ggtccatcgt gtccatcagc
     1501 gtgagctgcg gctgatcgac ctgctcgagg acgaggcgct gctgctcggg atccatgttg
     1561 gcgaggaaga tgtactggct ccggcggtag tcgtccggga tcgtggggcg gaagtccgtg
     1621 aacacgttca gctgcgtctc taacgtttcg cgcgagttca tgtcgtagtc ataccgtccg
     1681 gcccagcgga acgtgcttcc ttcgactacc tgcacgccac gcatatcgac atggcgctgg
     1741 cgcagaaaat cgagctccct ctggtcgaga tcgggtccga tgacggagac gaggttgacg
     1801 cgcgagtaga agcttgccgc cgcggcgaag tgggtggccg agccaccgag cgtcgatgcc
     1861 atggaaccga aaggggtctc gatgttgtcg agggcgatgg ttcctgcgac gagaatgctc
     1921 acgctgtctt ccttccctgc attgccctta ctttaggtag cggccgatta tcggcgcaag
     1981 ctcgcgcctc tttccgtccg aggcccgagc gagtggcgtc gcgaaggcgt cggcgagcgc
     2041 attttggcaa gcgcaaccat ccggcgcggg aatactgccc acgagcagcc ggacgatcgt
     2101 cttcgtggtc gcggcgttct tctggaggtt ctggaagatg aggttcgcat tcaccgactc
     2161 gtggccctcg tgccatgtat cgtaatcggt gacggccatg agggccgcgt agcacatctc
     2221 ggcctcccga gcgagctgcg cctccggcgc tgccgtcatg ccgatgacat cggcgcccca
     2281 gctgcggtac agctccgatt cggctcgagt cgagaaccgc ggtccattga tgacgacgca
     2341 tgtcccgccg aggtgcgtcg tgacgccggc ggcttccgcg ctggcgcccg cggcgcggct
     2401 gagccgggga cagaacggct cggcgaacga gatgtgcgct acgaggccgc gtccgaagaa
     2461 ggagccggga cgtcccgtcg ttcgatcgat gagctggtgc ggcaccacga tgtgctgcgg
     2521 cgcgatgtcc gctcgcaggc tcccgacggc gctgatcgat aggacctgcc gcacgcccag
     2581 cgacttcagg gcccagatgt tcgcttgcga ggggagctcg ttcgggggga tgccgtggtc
     2641 gcctccgtgg cgaggcagga acgcgatcct cacgccatcg agcgacccaa tcgtgatcgc
     2701 atcgctgggc atgccgaatg gcgtcgcgac ttcgacccgg acggcttcgg caagagactc
     2761 gaggtcgtat acgcccgtcc cgccgatgac agcgaagcga acgtcgtcct tcgagccgcc
     2821 acccatatcg aacgcagtat agcgcagacg ttccgctagt attgcagcag cgagaaggct
     2881 tcgtaggcgc gcagccgctc gcgaccgccg agcgccgtga gctcgatcac gaaggcgcag
     2941 cccgcgacca ccccgccgat gagctcgacg agcttggcgg cagcgtgcgc cgtgccgcct
     3001 gtcgccagca ggtcgtcgac gatgagcgcc cgctgaccat ggtcgagtgc gtcggcgtgg
     3061 atatcgagct ggctcgtgcc gtattcgagc gcgtactcca tcgacatgcg ctgcgccggc
     3121 agcttccccg gcttgcgaat gggaacgaac ggcagccgca ggcgatcggc gagcggggca
     3181 ccgaagagga aaccgcgcga ctcgatgccg acgatcgcgt caaggtgagc gtcgcggtag
     3241 cgcgcctcgt aggtgtcgag gacgtactgc atggcttccg gcgttcggag caggggcgtg
     3301 atatcgcgga agaggatgcc cggctggggg aagtcgggta tgtcgcgaac gtaggctcgg
     3361 aggtccatgg ttgccgctcc gcgcgaggtt gttcgcatcc taccgcctct ccttcgcgaa
     3421 cacgaggccc aacgccgccg gtccgtggta cgctcgcgcg agagatcacg ggaggaaggc
     3481 atgaagacgg ccctagttca gtacctctac gattaccagt acgcgcaact cctcacggcc
     3541 gatggccacg ccttcgttgc cgacgaaccg accgacagcg gtggcgacgg cctgggccct
     3601 gacccgtacg aactcttgct gtggagcctc ggcgcctgca ccgcgatgac gctcctcatg
     3661 tacgcgcggc gccacgacta tccgctcgcc gacatcgctg tccaggtgga gcacgaccgc
     3721 gtgtatccgg acgactccgc cgccaccgcc gagccgggag cggagcgcag gaagatcgaa
     3781 gtgctgcgcc gcaagatcac gctgcgcggc gacatcacgg acgagcagcg cgagcgactg
     3841 ctggagatcg cgcggaggtg ccccgtgcac cgcaccctcg cggccgcgcc cgaggtcgtc
     3901 gacacgatct cggtgggcga gtgagcggat cgcgagcgcc tgctaccaga cgtgcttcgg
     3961 ttgtacgccg agatcgatct cggagtcctc gacggcgacg cgaagggcag cgctgaagga
     4021 cttcatgatg cgtagactgc ggaggtacgc ctccagctcc gcggcgggga cgtctcgctc
     4081 gttccgctct tcggagtccg cgaaccaatg gctgacacgc acgtgggacc tggcgttcat
     4141 ggtgcctccc gggtctcgaa tcgccttatc tcagggccgg gcctatgatt tcgtagtgga
     4201 gaaccctacg tttccccttc tcgcactcaa tcctggctcg accggccgat aataaggtca
     4261 tgctgatctc attagaaacg aacggaaccg gaccaagttt cgggtacaca caacagaagg
     4321 tgcaccagat ggaaccgcgc gacgagggcg ccgctcgcga ggagtcccgc tctccgtttg
     4381 ccagggtaag cgaagagctc gcgcgggcac ggcgagaggc cgatcgcgtg ctgcgcgacg
     4441 cggagcgccg tgcggaggag atcctccacg aggccgagga gccccggggc gcgcccgtcg
     4501 agcggtcctt ggccgagctt ctgcatcagc tcggcgttca ggtcacgcag gtcgtcgcat
     4561 cggcggagga ggaccgccgt ctgctgcgtg agcagatgca gctcatccgc gaaatgctcg
     4621 gcgcgatgcg cgaagacatc gcctccctgc ggtcgcaggc cgctccccgg gtcgtcgcgc
     4681 cgcctccagt cgcgcctccg ccgcctatga cggaaccgcc ggtggcggaa gcgccggccg
     4741 ccgcgcccac acctccgcct ccggcgttcg agccgccgcc tccggcattc gagccggcgc
     4801 cgcccgagcc ttcggcgccg cttgtgcccg acctggaggg gacggtgttc gaggcgcgcg
     4861 ggcagtacgt gcaccttttc atcgagaacg tgcgcacgtt cgggcagctc acatcgatct
     4921 accaggcgct gctggcgatg gatagcatcc aggccatctc catcagccgc tatcagtccg
     4981 acgaggcgca cctgctgctc gacctgaagg tcgacgttgc ggcgggctcg gtcgtcgaga
     5041 cgctccgcgc cgcgggctac gacgcctcca tcgtcgaggc cgacgcgggc ctgctgacgc
     5101 tgcggctggc gttgtcctga gcgaacgcgg gtagcacgcc cgggagcgaa cgtataatcg
     5161 gcgcatgtac ttgcgccatc tcaccctgaa ccactaccgc acgtacgccc ggctcgagat
     5221 cgagctcgaa ccgggcgttt ccgtgttcat cggagagaac gcgcgcggca agacgaacct
     5281 gctggaggcc atctacctgc tcgcggtggg gcgcgtcgcc cgcgcggagc tcgacggcga
     5341 ggcgatcgcc tgggacgcgt ccgatcgtcc gcccttcgct cgggtcgcgg gggacgtggt
     5401 cgtggcgcgc ggcgagatgc gggcggaggc ggtgattgcc gcgcaccagg gttgggagga
     5461 ggagccttcg ccctcgcggc cgccccgcgc ctcgaagcgg ttccgcatca acggcgtgcc
     5521 ccgccgcctc gccgacttcg cgggaacgtt cgcggcgacg ctgttttccg tcgaggatct
     5581 cgccgtggtg aacggcccgc cctcgggcc
//
LOCUS       DATMBB010000273         4333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_285955, whole genome shotgun sequence.
ACCESSION   DATMBB010000273 DATMBB010000000
VERSION     DATMBB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285955"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VNL92_05880"
     CDS             <1..1025
                     /locus_tag="VNL92_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWO94275.1"
                     /translation="VVIHELGHFITAKLAGVSVLEFGIGYPPKIWGKKFGETEYTINW
                     LPLGGFVRLVGEEDPSDPRSLARRPRWIRLIVLASGSIMNLLLPILLFAIAFMIPQEI
                     SIGRPIIADVVPDSPAAEAGLQPGDVIYEAEGRETRNLADLSRAIQLNLGEQLDLKVK
                     RGTEFVQLDAKARWVPPDGQGPLGIRIASQYPFTETESYPVWEAVPKGFQATWETVIL
                     ARNQIIALIKGGGESEGPAVSGPVGIAQATGEVVEEAGWEPLLEFAALLSLNLGVVNI
                     LPLPMLDGGRIAFVVLEILRGGKRISPQKEGLVHFIGFVLMISFVVIVSYFDVLRLIS
                     GDNILE"
     gene            1093..2283
                     /gene="ispG"
                     /locus_tag="VNL92_05885"
                     /gene_synonym="gcpE"
     CDS             1093..2283
                     /gene="ispG"
                     /locus_tag="VNL92_05885"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWO94276.1"
                     /translation="MDEPLTPIRRVSKPVYVGDVQVGGDAPVRVQSMTNTDTANAEAT
                     IEQIAELADLGCEIVRIAVPDRQAAAAVPEIVQRSPVPLIADIHFDYRLALESIRGGI
                     HGLRLNPGNIRDAEKVRAVVREAKERDIPIRIGVNAGSLPPIGWLDEGELPPPTWQRM
                     VDAALWEIGILHEMDYDNIKISLKAFDVPTMVRAYRALAQQVPYPLHLGVTEAGTPRA
                     GSVRSAIGIGILLAEGIGDTIRVSLAADPKEEVPVCWDILASLNLRQRGPTIVACPTC
                     GRIEIDLIPLANQVEEYFGKRGYPLTVAVMGCVVNGPGEAKEADIGIAGGKGRGVIFR
                     GGEVIRTVNEAEFLPALIEEGERIIAEKEGRELAPRSSTDDLILPVVPAPSGPRIGRG
                     ARLA"
     gene            2280..3200
                     /locus_tag="VNL92_05890"
     CDS             2280..3200
                     /locus_tag="VNL92_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWO94277.1"
                     /translation="MTSTSRALALSTMWSQQPRFAHDLGEFARTAVELGYTHVELSYI
                     AREEGIEQVRRSGVPVTSLHAPAPLIEIRGRRNFDLNLAAEDEDERCQAVAHTKRTIE
                     VAASLGARAIVVHLGAVEGEARQSEVQLRRLVREVAPTEDIEHAREATLRARAEGQPK
                     AFAQAKRSLAELASHAERYRVTIGLEDRFHVHEIPNVDEALELLAAYPPDVVGYWHDV
                     GHAEVLDRLGVIPHERWLRELGERCVGAHLHDVQGIIDHRAPGCGDADWDYVARYLPD
                     EAIRTFEINQQIPDDEVRAAIPFLRARGIV"
     gene            3283..>4333
                     /gene="proS"
                     /locus_tag="VNL92_05895"
     CDS             3283..>4333
                     /gene="proS"
                     /locus_tag="VNL92_05895"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWO94278.1"
                     /translation="MRLSSLFGKTLRQAPAEAETPSHRLILRARLAEQIAAGIYAFLP
                     LGWAVLRRVEQIVREEMDREGGQEVRLPILTPVEQWESTGRRTVMDDILLVTEDRRGR
                     ELVLGPTHEETFTELVRRQLQSYRALPLRLYQIEAKFRDEPRPRGGLIRLRQFTMKDL
                     YSFDADQAGLTESYAAMDRAYRRIFARCQVPVVAVQAHSGSMGGSESQEFMLLTDIGE
                     DTVVTCPACGYAANAERAEIRRSQPDRPNEPLPIEEVSTPGARTIEEVARFLGIGRDE
                     TLKAVFYVADGEPVFVAIRGDLDVNEVKLAAVLGARELRLMSDDEVRAAGLTAGAASP
                     IGVHGMRVIADESAAW"
BASE COUNT          755 a         1481 c         1379 g          718 t
ORIGIN      
        1 tcgttgtcat ccacgagctc ggccacttca tcaccgccaa gctcgccggc gtgagcgtgc
       61 tggagtttgg cataggctat cccccgaaga tatgggggaa gaagttcggg gagacggagt
      121 acaccatcaa ctggctcccg ctcgggggct tcgtccggct cgtgggcgag gaggatccga
      181 gcgatccgcg cagccttgcc cgccggcctc gctggattcg cctcatcgtg ctggcatcgg
      241 gttcgatcat gaacctgctg ctccccatcc tcctcttcgc gatcgcattc atgatcccgc
      301 aggagatctc gatcggacgg cccattattg cggacgtcgt gcccgacagt ccggcggccg
      361 aggccgggct ccaaccgggg gacgtcatct acgaagcgga aggtcgcgaa acccgcaacc
      421 tcgccgacct ttcacgcgcc attcagctca acctgggcga gcagctcgat ctaaaggtga
      481 agcggggcac ggagttcgtg cagctcgatg cgaaggcccg gtgggtaccg ccggatggcc
      541 aaggaccact cggcatacgc atcgcgtcgc agtatccatt cacggagacc gagtcgtacc
      601 cggtctggga ggcggtgccg aagggattcc aggcgacctg ggagaccgtc atcctcgcgc
      661 gcaaccagat catcgcgctc atcaagggtg gcggcgaatc ggaggggccg gctgtgagtg
      721 ggcccgtcgg catcgcgcag gctacgggcg aggtcgtgga ggaggcgggc tgggagccgc
      781 tcctggagtt cgcggcgctg ctgagcttga acctaggcgt cgtgaacatc ctgcccctgc
      841 ccatgctcga tggcggccgc atcgccttcg tggtcctcga gattctgcgc ggcggcaaac
      901 gcatctcgcc ccaaaaggaa ggcctggtcc acttcatcgg cttcgtgctc atgatctcct
      961 tcgtcgtcat cgtcagctac ttcgacgtgt tgcgcctcat ctcgggcgac aatatcctcg
     1021 aatagcctct cccttacgat ccgataatcc tccactttgg gaaatgaggt acgatatccg
     1081 gcgggagaac gcatggacga acccctgacc ccgatacgcc gcgtctccaa gcctgtgtac
     1141 gtcggcgacg ttcaggttgg tggcgacgct cctgttcgcg tccagtcgat gacaaacacc
     1201 gacaccgcca acgccgaggc gacgatcgag cagatcgccg aactggccga tctcggctgc
     1261 gaaatcgtgc gcatcgccgt tcccgatcgc caggccgcgg ccgccgtacc cgaaatcgtg
     1321 cagcgcagcc ctgttccgct catcgccgac atccacttcg actatcgcct cgcgctggaa
     1381 tcgatccggg gcggcatcca cggtctccgc ctcaacccgg gcaacatccg cgacgctgag
     1441 aaggtgcggg ccgtggtccg ggaggcgaag gagcgcgaca ttcccatccg tatcggcgtc
     1501 aacgccggat cgctgccgcc catcggatgg ctggacgaag gcgagttgcc accacccacg
     1561 tggcagcgca tggtcgatgc ggcgctttgg gaaatcggca tcctgcacga gatggactac
     1621 gacaacatca agatctcgct caaggccttc gacgtaccga ccatggtgcg cgcctatcgt
     1681 gccctcgcgc agcaggtacc gtatcccctg catctgggag tcaccgaagc aggcacgccg
     1741 agggccggat cggttcgcag tgccatcggc atcggcatcc tcctcgcgga gggcatcggc
     1801 gacaccattc gcgtttcgct cgccgccgac ccgaaggaag aagtgcccgt ctgctgggac
     1861 atcctcgcat cgctgaacct gcgccaacgc ggtcccacca tcgtcgcttg tccgacctgc
     1921 ggaagaatcg agatcgactt gatcccgttg gcgaaccagg tcgaggagta cttcggcaag
     1981 cgaggctacc cgctcaccgt tgccgtcatg ggttgtgtgg tcaacggccc cggagaagcg
     2041 aaggaggccg acatcggcat tgccggaggc aaggggcgcg gggtcatctt ccgaggcggc
     2101 gaagtcatcc gcaccgtgaa cgaggccgaa ttcctccccg ctctcatcga agagggcgag
     2161 cgcatcatcg ccgagaagga agggcgcgag cttgcccccc gctcctccac ggatgacctc
     2221 atcctccccg ttgtgccggc gccgtccggc ccgcggatcg gccggggcgc tcgcctcgca
     2281 tgacttcaac ctcgcgggca ctcgctctgt cgaccatgtg gtcgcagcag ccgcgctttg
     2341 cccacgacct tggcgagttc gcgcggaccg cggtcgagct gggctacacg cacgtcgagc
     2401 tcagctacat cgcacgagag gaaggcatcg agcaggtccg ccgaagcggc gtgccggtca
     2461 ccagccttca tgctcctgcg cccctcatcg agatccgagg ccggcgcaac ttcgacctca
     2521 accttgcggc agaggacgag gacgagcgct gccaggccgt cgcccatacc aagcgaacca
     2581 tcgaagtagc agcctcgttg ggcgcccggg ccatcgtggt ccacctcgga gcggtcgagg
     2641 gcgaggcgcg ccaatccgag gtgcagctgc gccggctcgt gcgtgaggtc gcaccgacgg
     2701 aggacatcga gcacgcgaga gaggccaccc ttcgggcgcg cgccgagggt cagcccaagg
     2761 ccttcgccca ggccaagcgt tcgcttgccg agctcgcaag ccatgcggag cgataccgcg
     2821 tgacgatagg cctcgaggac cgctttcatg tccatgagat cccgaacgtc gacgaagcgc
     2881 tcgaactcct cgccgcctat ccgccggacg tcgtcggcta ctggcacgat gtcggccatg
     2941 ccgaagtgct cgatcgactc ggcgtcatcc cacacgagcg ctggctccgc gagctcggtg
     3001 agcgctgcgt aggggcgcac ctccatgacg tgcaggggat tatcgaccac cgcgcgcccg
     3061 gttgcggcga tgccgattgg gactacgtgg cacgatacct gcccgatgag gccatccgca
     3121 cgttcgaaat caaccagcag ataccggacg acgaggttcg cgcggccatc cccttcctcc
     3181 gagcaagggg tatcgtctga cctgccccac ctgaagccgg tggcccgtgg cgcttcgctc
     3241 ccctatactc gacccatccc ccacgaggtc ggtaccttgg ccatgcgact ctcgtcgctg
     3301 ttcgggaaga ccctccggca ggcgccagct gaggccgaga cgccgagcca tcggctcatc
     3361 ctacgcgcgc gcctcgcgga acagattgcg gccggaatct atgcctttct cccgctcggt
     3421 tgggccgtgc tgcgccgcgt cgagcagatc gtccgcgagg agatggaccg ggaaggcggc
     3481 caagaggtac gactgcccat cctcacgcct gtagagcagt gggaatcgac ggggcgccga
     3541 accgtcatgg acgacatcct cctcgtgacg gaggaccgcc gaggccgcga gcttgtcctc
     3601 gggcccacgc acgaagagac gtttaccgaa ctcgtgcgcc gtcagctcca aagctatcgc
     3661 gccctcccgc tgcggctgta tcagatagag gcgaagttcc gcgacgagcc ccgccctcgc
     3721 ggcggtctca tccgcctgcg gcagttcacc atgaaggacc tctacagctt cgatgccgac
     3781 caagcgggcc tgaccgagag ctacgcggct atggaccgag cgtaccggcg catcttcgcc
     3841 cgctgccagg tccccgtcgt agccgtgcaa gcgcactccg gctcgatggg cggcagcgag
     3901 tcccaggaat tcatgctgct caccgacatc ggcgaggaca ccgtggttac ttgcccggct
     3961 tgtggttacg ccgcgaacgc cgagcgcgcc gagatccggc ggagtcagcc ggaccgcccg
     4021 aacgaaccgc tgcccatcga ggaagtatcg acgccgggcg cccgcacgat cgaggaagtg
     4081 gcgcgcttcc tcgggattgg ccgcgacgag accctgaagg ccgtcttcta cgtcgcggat
     4141 ggcgagccgg tgttcgtggc catccgcggc gacctcgatg tcaatgaggt caagcttgcg
     4201 gccgtcctcg gggcacgcga gctccgtctc atgtccgatg acgaagtgcg ggctgccggc
     4261 ctcaccgccg gcgccgcctc gcccatcggc gttcacggca tgcgggtgat cgccgacgaa
     4321 tcggcggcgt ggc
//
LOCUS       DATMBB010000274         3543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_477369, whole genome shotgun sequence.
ACCESSION   DATMBB010000274 DATMBB010000000
VERSION     DATMBB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477369"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNL92_05900"
     CDS             <1..492
                     /locus_tag="VNL92_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO94279.1"
                     /translation="EITLGVGRVAMPSYYPRHRLNSGTTGCMGTGVPYAIGAKLARPN
                     SQVVAILGDYAFGASGMEVETAARVGANVVFVIDNNQGIAGHIIHKSMFGTEHYGIST
                     LLPARYERMIEMVGGHAERVEDPDEIRPAVDRALNSGKLALVNVMTDPELGGARGANY
                     LMG"
     gene            complement(505..1215)
                     /locus_tag="VNL92_05905"
     CDS             complement(505..1215)
                     /locus_tag="VNL92_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HWO94280.1"
                     /translation="MVLTASQSAPPHDDDTPQGQEPVHSGSEAPQLLPNLIPLEPQDV
                     QFERAFGGRVSLRFTTMSWNAGAGPFEIVPGQTDADSQLVYQRIYRADGSYDDRPAGR
                     FVFHPQHQHMHFDNYATYALYSVDAGDTYRTESKTSFCLRDSRLYEPELAGAPRLPAY
                     QECENAKQGLSVGWSDRYGYDLPGQALDVTDLPPGYYELTIVVDPENQIVESDEGDNI
                     AGWGLYLDVEEDRIDILY"
     gene            complement(1225..1530)
                     /locus_tag="VNL92_05910"
     CDS             complement(1225..1530)
                     /locus_tag="VNL92_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94281.1"
                     /translation="MSSSRRAVFAAFAASWIAIVGGVPRITQADPAVHPNGAYGPGAN
                     AIVVEGGGTPGDIAKVVAEDSRMRVDALWLLIGGKWLFYLPQAPLVEGGLQTFPPNA"
     gene            complement(1673..3058)
                     /locus_tag="VNL92_05915"
     CDS             complement(1673..3058)
                     /locus_tag="VNL92_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWO94282.1"
                     /translation="MRGFVVAGTTSGVGKTTITSGLISALRARGCRVQPFKAGPDYID
                     PSYHARAAGRACRTLDSWMLPHDVLRALFAHASRDADIAVVEGVMGLFDGRSGEGEAG
                     STAEIAKLLGLPVILVVDAAKVARSVAAQVLGYRQFDADVRIAGVILNNVAGPGHSAL
                     CAEPIVAATGIPVLGALPRSDELRLPERHLGLIPTVEGRTAGGFFEAARAMVEEHVDL
                     TALEAIAASAVLRAPVVGWEALHRPASKPERVRLAVAQDEAFSFIYPENLELLQAAGA
                     DIVPFSPLRETQLPHDASGVYLGGGFPELYAEALAANEPMLRSVRDAARMGLPIYAEC
                     GGLMYLGEALVDQAGRSHRMAGVVPVRSSMERTRLTLGYRTVRARADGPLLPAGRSVR
                     GHEFHWSELDAPVPAATAAYQVVETGALEGYAKDNICASYIHLHFAADLSIAPRFVEA
                     CRIAPRRSAVL"
     gene            complement(3060..>3543)
                     /gene="cobO"
                     /locus_tag="VNL92_05920"
     CDS             complement(3060..>3543)
                     /gene="cobO"
                     /locus_tag="VNL92_05920"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWO94283.1"
                     /translation="KGKTTAALGLLLRGWGRDWRVCMLQFIKAKTGNYGENKAARKLG
                     VELIPLGDGFTWLSKDIEKDKALARECWELCKQKIASDEYDLVIMDELTYPLVYGWLP
                     TSEVIETLRARPAGLHVVITGRDAPQELIDFADLVTEMREVKHPYRTQGIRAQPGIEF
                     "
BASE COUNT          594 a         1200 c         1171 g          578 t
ORIGIN      
        1 gagatcacgc tcggtgtggg acgcgtcgcg atgccgagct actatcctcg gcaccgcctg
       61 aactcgggca cgaccggctg catggggacg ggcgtgcctt acgcaatcgg ggccaagctc
      121 gcccgcccga actcgcaggt cgtcgccatc ctcggcgact atgccttcgg cgcgagcggt
      181 atggaggtgg agaccgccgc ccgcgtgggg gcgaacgtcg tcttcgtcat cgacaacaac
      241 cagggcatcg cggggcacat catccacaag tcgatgttcg ggaccgagca ctacggcatc
      301 tcgacgctgc tgccggcccg ctacgagcgc atgatcgaga tggtgggagg ccacgcggag
      361 cgcgtcgagg acccggacga gattcgaccc gccgtggacc gcgccctcaa ttcgggaaag
      421 ctcgcgctcg tgaacgtgat gacggacccc gagctcggcg gcgcccgggg cgccaactac
      481 ctcatgggct gacgtacccg cggctcagta gaggatgtcg atcctgtcct cttcgacatc
      541 aaggtaaagc ccccatccgg cgatgttgtc gccttcgtcg gactcgacga tctggttctc
      601 cgggtcgacg acgatcgtga gttcgtagta gcccggtggc aggtccgtga catcgagcgc
      661 ctgccctggg aggtcgtacc cgtaccggtc ggaccagccg actgagaggc cttgctttgc
      721 attctcgcac tcctggtagg ccgggaggcg gggcgctccc gccagctccg gctcatacag
      781 acgcgagtcc cgaaggcaga aggaggtctt gctctccgtc cgatacgtat cgcccgcgtc
      841 caccgagtag agcgcgtacg tcgcgtagtt gtcgaagtgc atgtgctgat gttgagggtg
      901 gaatacgaac cggcccgcgg gcctatcgtc gtacgacccg tcggcccggt agatgcgctg
      961 atagacgagc tgcgagtcgg cgtccgtctg gcccggcaca atctcgaacg gacccgcgcc
     1021 agcgttccac gacatcgtgg tgaatcgcaa ggacaccctt cccccgaacg cgcgctcgaa
     1081 ctgcacatcc tgcggctcca aggggatgag attcggcaga agctgcggcg cttcggaccc
     1141 ggagtgcact ggctcctggc cttgcggcgt gtcgtcatcg tgcggaggtg cgctttggct
     1201 cgccgttaga accacgatgg cgctctatgc gttgggtggg aaggtttgca ggccgccttc
     1261 gaccagcggt gcttgcggaa ggtagaacag ccacttgccg ccgatgagga gccagagggc
     1321 atcgacgcgc atgcgactat cttcggcgac gaccttcgcg atatcacctg gcgtgccgcc
     1381 gccctcgacg acgatggcgt tcgcgcccgg gccgtaggct ccattcggat ggacggcagg
     1441 atcggcctgg gtgatacggg ggacgccccc gacgatcgca atccacgacg ccgcaaacgc
     1501 cgcgaacacg gcgcgcctcg aactcgacat gcccctgtct ttccctcctg ccgcccgcgg
     1561 cagatcaatc ttctcgaacc atggtcgcgg agtaccggcg agccaaggtg aagcgaaggt
     1621 tggccaagcg tggagcgtgt cgaagctcgt ctcgccggtc gcagggtccg aactaaagta
     1681 ccgcactccg ccttggagcg atacggcagg cttcgacgaa tcgcggggcg atgctcaagt
     1741 ccgcggcgaa gtgaaggtgg atatacgagg cacagatgtt gtccttcgca tatccctcga
     1801 gtgccccggt ttccaccacc tggtatgcgg ccgtcgccgc gggtaccggc gcatcgagct
     1861 ccgaccagtg gaactcgtgt ccgcggacgc tgcgtcccgc cggaagcagc ggcccatcgg
     1921 cacgggcgcg cacagtgcga tagccgagcg tgaggcgcgt gcgttccatc gaggatcgga
     1981 ccgggacgac gccggccatc ctgtgtgatc gcccggcctg atcgacgagc gcctcgccca
     2041 ggtacatcag accgccgcac tccgcgtaga tgggcagccc catcctcgcg gcgtcacgta
     2101 ccgagcgcag catcggctcg ttggcggcca gcgcctccgc gtacaattcc gggaagcctc
     2161 caccgaggta gacccccgat gcgtcgtgcg gaagctgagt ctcccgcagc ggactgaagg
     2221 gcacgatgtc cgcgcctgcg gcctggagga gctcgagatt ctccgggtag atgaagctga
     2281 acgcctcgtc ctgcgctacc gcgaggcgca cgcgctccgg tttcgaggca gggcgatgga
     2341 gtgcttccca gccgaccacg ggcgctcgta agacggccga cgcagcgatg gcctcgagcg
     2401 ccgtgagatc gacatgctcc tcgaccatcg ctcgcgccgc ctcgaagaag ccgccggcgg
     2461 tccgcccttc caccgtcggg atgaggccga gatgccgctc cggcagccgg agctcgtcgg
     2521 agcgcggcag ggcgccgagc accgggatgc ccgtcgcggc gacgatcggc tccgcacaga
     2581 gggcggaatg gcccggaccc gcgacgttgt tcaggatgac ccccgcgatc cggacgtccg
     2641 cgtcgaactg ccggtaccca agcacctgcg ccgcgacgct ccgcgccacc ttcgcggcgt
     2701 ccacgacgag gatgaccggt aggcccagca gcttcgcgat ctctgcggtg ctgcccgcct
     2761 ctccttcgcc ggaacggccg tcgaacaggc ccatcacgcc ttcgaccacg gcgatgtcgg
     2821 cgtcacggga agcgtgcgcg aagagcgccc gcagcacgtc atgcggcagc atccacgaat
     2881 cgagcgtgcg gcaagcacgc ccggccgcgc gagcgtggta cgagggatcg atgtagtccg
     2941 gcccggcctt gaacggctgc acgcggcagc cacgcgcccg gagcgccgaa atcagcccgg
     3001 acgtgatggt cgtcttcccc acgccgctcg tggtccccgc gacgacgaag ccgcgcacgt
     3061 cagaactcga ttccgggctg ggcgcgaatg ccttgcgtgc gatacgggtg cttcacttcg
     3121 cgcatctccg tcacgaggtc ggcgaagtcg atgagctcct ggggcgcgtc ccgccccgtg
     3181 atgacgacat ggagacccgc tgggcgcgcg cgcaaggtct cgatgacctc cgatgtaggc
     3241 agccagccgt acacgagggg ataggtcagc tcgtccatga tcacgaggtc gtactcgtcg
     3301 ctcgcgatct tctgtttgca cagctcccag cactcccggg cgagggcctt gtccttctcg
     3361 atatccttcg aaagccaggt aaagccatcg ccgagcggga tgagctcgac gccgagcttg
     3421 cgggctgcct tgttctcgcc gtagttccct gtcttcgcct tgatgaactg cagcatgcac
     3481 acacgccagt cgcgtcccca gccgcgcaga aggaggccca gggccgccgt cgtcttgccc
     3541 ttg
//
LOCUS       DATMBB010000275         6120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_70695, whole genome shotgun sequence.
ACCESSION   DATMBB010000275 DATMBB010000000
VERSION     DATMBB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70695"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1297
                     /locus_tag="VNL92_05925"
     CDS             20..1297
                     /locus_tag="VNL92_05925"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWO94284.1"
                     /translation="MEEAIEDYRQGKFVIIVDDEDRENEGDLTIPAQFVTAEHINFMA
                     RFGRGLICMPMTGQRLEELKIPMMVQNNDAHFGTPFTVSVEAAHGVTTGVSAADRAHT
                     CRVLIDPRSKPEDIAMPGHLFPLRARDGGVLVRAGQTEATVDLCKLAGLYPAGVLCEV
                     MRPDGEMARMPDLIRFARRHQIRIITVADLIAYRHRQEKLVERVAETVMPTSWGEFRV
                     VAYRSRIDPDEHVALVRGEIDPDVPTLARVHSQCVTGDVFHSLRCDCGEQVDIALQRI
                     ADEGRGVFVYMRQEGRGIGLHNKLRAYALQDNGLDTVEANEALGFPADRRDYGIGMQI
                     LVDLGLRRIKLLTNNPAKRAGLEGYGLEVVERIPIITKPNPYNVRYLDTKRRKLGHLL
                     DDAAATAAASGGTRKPRRAASPSMGNGAGDVVG"
     gene            1290..1760
                     /gene="ribH"
                     /locus_tag="VNL92_05930"
     CDS             1290..1760
                     /gene="ribH"
                     /locus_tag="VNL92_05930"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWO94285.1"
                     /translation="MGRRFEASLSGEGLRLAIVVARFNALVTERLLEGAREGLRRHGV
                     RDEDVDEAFVPGSFELPLAARKLAATGRYDAVICLGAVIRGETTHDQYISSQAAAGIQ
                     RVALDCAVPVVFGVLTPNTLEQAFDRAGGKSNKGHEAAVTAIEMANLLRAIDSA"
     gene            1770..2219
                     /locus_tag="VNL92_05935"
     CDS             1770..2219
                     /locus_tag="VNL92_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWO94286.1"
                     /translation="MRGVSISFRSNGPQDSPDFGPWLMGLLIRWAINAVALVVAAALV
                     PGIELEGWESTLITAGIFGIVNALIRPFVMCITCLLQVLTLGLFTLIVNAAMLALTAW
                     AADGLGLEFEVDGFWAAFLGALVISLVSFVLTRVAPPAGRGSERSRH"
     gene            2311..2733
                     /locus_tag="VNL92_05940"
     CDS             2311..2733
                     /locus_tag="VNL92_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWO94287.1"
                     /translation="MTTTIDRLAGVPLFRNLPKKSLERLQRVVATRTFGSGEDIVKEG
                     EIGSGFFLITEGAAEAVRGDTKLATYGPGDFFGEMALLGNHPRSATVRSTAGTTCLAL
                     TRWDFVAELRANPDLAVEMLEVMAARLREADKRLAEHD"
     gene            2916..3599
                     /locus_tag="VNL92_05945"
     CDS             2916..3599
                     /locus_tag="VNL92_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWO94288.1"
                     /translation="MSDPVTTQKASRLLTEVEAKELLSSAGIAVAATRLATSAADASK
                     IAEQLGFPVVIKIVSPDIAHKSDIGGVRLNLNSPADVAAAYEAMMADVAKVAPSAKIL
                     GAAVQHMEPAGTEVIMGMTKDPQFGPVLMFGLGGIFVEVLQDVAFRIVPLERRDARQM
                     IREIKGYAVLEGVRGQDPSDIEALEDMLLKLSAFVEARPEIAELDLNPVFAYKKGALA
                     VDARIVVEE"
     gene            3639..5081
                     /locus_tag="VNL92_05950"
     CDS             3639..5081
                     /locus_tag="VNL92_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWO94289.1"
                     /translation="MTTRVRSRLERVFNPRTLAVIGDKQISGYMWLRNNKDFAGKLYS
                     VQIDEREIPGIEAMGIPNYKSLLDIPEDVDYAIIAVPRQVAPRVVQDCVAKGVGGCML
                     FTSGFAETEEEEGVHLQRTLAELARAGDLALIGPNCMGIHVPRLGIRHSPDQPVGPDA
                     GGPVGFIAQSGTHAGFFTQAAPEHGIRISKSVSFGNAIVLDASDYLEYLAVDDETEII
                     GMYIEGVRDGRRFFETLRQTARRKPVVIWKGGQTEAGQRAIYSHTASLAAPMRIWDAM
                     TKQVGAVSTNNLDETVDVMKALLHAKPATRMAAGLIAMTGGPSVGITDAFARAGFDVP
                     RLHEGSYEKLSQFFNVIGGSYRNPFDAGGTIGMGGQPQNLDKLLEIIDEDDNIAVVAM
                     DLGFGFAGRRWKERPEMLDAMLDTLAGFRDRSSKPLALILQPAHLEDLAVEMRRRVQE
                     RGFAVFASYERAADAMAKAIAYHRFRAELD"
     gene            5197..>6120
                     /locus_tag="VNL92_05955"
     CDS             5197..>6120
                     /locus_tag="VNL92_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWO94290.1"
                     /translation="MQTHISYVLIAGEHVYKLKKPLDLGFLDFRTLDARRIACEREVA
                     LNSRLCAEAYLGVVPIIEMPEGPAIGESAEGREPLDWAVHMRRLPDESFLDRRLLDGH
                     VPANTAALLAQRLVEFHEAAATNDEVARYGSVELIAANWQENFDQVRPYIGRTIAQAD
                     FDSLVSYVDRVLREDAGTFARRAKRDRVRDGHGDLRCDSVSLGDDGSLCIIDCIEFND
                     RFRYADVASDLAFLLMDLDRLGASWLADEVLTQYLARSFDESLPLVLPFYRCYRAFVR
                     GKVHGFEMDEAEIPAEQRARAELTARRCFDLA"
BASE COUNT         1067 a         2041 c         1984 g         1028 t
ORIGIN      
        1 cacgaaggtt cgagccacta tcgaggaagc tatcgaggac taccgccaag gtaagttcgt
       61 gatcatcgtg gacgacgagg atcgcgagaa cgagggcgat ctcaccatac cggcccagtt
      121 cgtaacggcg gaacacatca acttcatggc caggttcggg cgaggcctca tctgcatgcc
      181 gatgactggc cagcgcctcg aggagctcaa gatcccgatg atggtgcaaa acaacgacgc
      241 gcacttcggg acacccttca ccgtgtcggt cgaagccgcg catggcgtga cgacgggcgt
      301 ctccgccgcc gaccgcgccc acacctgccg cgtcctcatc gacccgcggt cgaagcccga
      361 ggacatcgcg atgcccggcc acctcttccc cctgcgcgcg cgggatggcg gcgttctcgt
      421 ccgcgccggc cagacggagg cgaccgtgga tctatgcaag cttgccggcc tgtaccctgc
      481 tggcgtcctc tgcgaggtca tgcggccgga cggcgagatg gcgcgcatgc ccgatctgat
      541 tcgcttcgcg cggcgtcacc agatccgcat catcaccgtc gccgacctca tcgcctatcg
      601 ccaccgccag gagaagctcg tcgagcgcgt cgccgaaacc gtgatgccga cctcgtgggg
      661 agagttccgt gtcgtcgcat acaggagccg aatcgacccc gacgagcatg tcgcgctcgt
      721 acgcggggag atcgacccgg atgtgccgac cttggcacgg gtgcacagtc agtgcgttac
      781 cggcgacgtg tttcacagcc tgcgctgcga ctgcggcgaa caggtcgaca tcgctctcca
      841 gcgcatcgcg gatgaaggac gtggcgtatt cgtctacatg cgacaagaag ggcgcggcat
      901 cggcttgcac aacaagctgc gcgcctacgc cctgcaggac aacggcctcg acaccgtcga
      961 ggcgaacgag gcgctcgggt tccctgccga ccggcgcgac tacggcatcg gcatgcagat
     1021 cctggtcgat ctcggcctgc ggcgcatcaa gctgctcacc aacaacccgg cgaagcgagc
     1081 cggcctggag ggctacggcc tggaggtcgt cgagcgaatc cccatcatca ccaagcccaa
     1141 cccttacaac gttcgatatc tcgacacgaa gcgacgcaaa ctcggacatc tgctcgatga
     1201 cgctgcggcg acggcggcgg cttccggagg gacacgaaag ccgagacgcg cggcatcgcc
     1261 gagcatgggg aacggcgccg gggacgtcgt tgggtaggcg tttcgaggca tcgctgtcgg
     1321 gggaaggcct gcgactcgcc atcgtcgtgg cgcgattcaa cgcgctcgtc acggaacgct
     1381 tgctcgaagg cgcgcgcgaa ggcctgcgcc gccacggtgt gcgtgacgag gatgtcgacg
     1441 aggcgttcgt gccaggctcc ttcgaattgc cgctggcggc gaggaagctc gctgccacgg
     1501 ggcggtacga tgctgtcatc tgcctcggcg ccgtcatccg gggcgagacg acgcacgacc
     1561 agtacatctc atcgcaggcg gcggccggca ttcagcgggt ggcgctcgat tgcgccgtgc
     1621 ccgtcgtctt cggcgtgctg accccgaata ccctcgagca ggcgttcgat cgcgccggtg
     1681 gcaagagcaa caagggccac gaggccgcgg tgacggcaat cgagatggcg aacctgctac
     1741 gcgccatcga ttccgcctaa ccgtcgccca tgcgcggtgt ttccatctcc ttccggtcga
     1801 acggccctca ggactcgccc gacttcggcc cctggttgat gggcctcctc atccgatggg
     1861 ccatcaacgc cgttgcgctc gtcgtagcag cagcgctcgt ccctggaatc gagctcgaag
     1921 ggtgggaatc cacgctgatt acggcgggga tcttcggcat cgtgaacgcg ctcatccgtc
     1981 cattcgtgat gtgcatcacc tgtttgctgc aagtgctgac cttggggctc ttcaccctca
     2041 tcgtcaacgc ggccatgctt gccctcacgg cttgggcggc ggacggcctc ggtctggagt
     2101 tcgaggtcga tggcttctgg gcggcctttc tcggggcgct cgtcatcagc ctcgtgagct
     2161 tcgtcctcac gcgcgtggcg ccgccggcag ggcgcgggtc ggagcgatca cgacactaga
     2221 cagcgtgggt cgatgcacct acaatcgcgc cacccggaac gaggccacgg tctcgccgat
     2281 tcggggacga gagagggagg gagcgtcacc atgaccacta ccatcgaccg cctggcgggc
     2341 gttccgctct tccgcaactt gccgaagaag tcgctcgagc ggctgcagcg cgtggtcgcg
     2401 acgcgcacct tcggcagcgg ggaggacatc gtcaaggaag gggagatcgg ctccgggttc
     2461 ttcctcatca ccgagggcgc cgccgaggcg gttcgcgggg acaccaaact cgcaacgtac
     2521 gggccgggcg actttttcgg ggagatggcc ctgctgggga accaccctcg cagcgccacc
     2581 gtgcgctcga cggcgggcac gacgtgccta gcgctgacgc ggtgggactt cgtcgcggag
     2641 cttcgcgcca atcccgactt ggccgtagaa atgctggagg tgatggcagc gaggttgcgc
     2701 gaggcagaca agcggctcgc ggagcacgac taggggagcc gcccgtcgct caggtggctg
     2761 cggccgcccc ctcgcaatga tctgtgcggg aagtcgcgca cggtgacact ccgcccacca
     2821 ccccgaccga tgctcgccgt ttgacgcctt ggcggccttc ccgtaccata cgtgcggccc
     2881 aaagatgggc acttctcggc atccgaggcc atcccatgag cgacccggtc accacccaga
     2941 aggcaagccg gctcttgacc gaggtcgagg ccaaggagct gctgagctcg gccggcatag
     3001 cggtagcggc gacgcggctg gcgacgagcg ccgccgatgc ctcgaagatc gcggagcagc
     3061 ttggcttccc ggtcgtcatc aagatcgtgt cgccggacat cgcgcataag agcgacatcg
     3121 gcggcgttcg cctcaacctc aattcgcctg cagacgtggc tgccgcctac gaggcaatga
     3181 tggcggacgt agcgaaggtc gcgccctcgg ccaagatcct cggagcggcc gtccagcaca
     3241 tggagccggc cggcaccgag gtcatcatgg gcatgacgaa ggatccgcag ttcggccccg
     3301 tcctcatgtt cggcctcggc ggcatcttcg tggaggtcct gcaggacgtg gcctttcgga
     3361 tcgtgccgct ggagcgccgc gacgcgcgtc agatgatccg cgagatcaag ggctacgctg
     3421 tgctcgaggg cgtgcgtggc caagatccgt ccgatatcga ggcactcgag gacatgctac
     3481 tgaagctatc cgccttcgta gaggcacgcc ccgagatcgc cgagctggac ctgaatcctg
     3541 tattcgccta caaaaagggc gcgctcgccg tcgatgcccg catcgtcgtc gaagagtagc
     3601 ggcgctccga tcgagcggga gccgaaagga gcaacgtctt gacgacccga gtccgcagcc
     3661 gcctggagcg cgtgttcaat ccgcgcacgc ttgccgtcat cggcgacaag cagatcagcg
     3721 gctacatgtg gcttcgcaac aacaaggact tcgccggcaa gctgtactcc gttcagatcg
     3781 acgagcgcga gattcccggc atcgaagcca tgggcatacc gaactacaag agcctcctcg
     3841 atatcccgga ggacgtcgac tacgcgatca tcgcggtccc tcggcaggtc gcgccgcgcg
     3901 tcgttcaaga ttgcgtcgcc aagggcgttg gcggctgcat gctcttcaca tcgggtttcg
     3961 ccgagacgga agaggaggaa ggcgttcacc tccagcgcac actcgcggag ctcgcccgcg
     4021 cgggcgacct ggcgctcatc gggccgaact gcatgggcat ccacgtgccg cggctcggca
     4081 ttcggcattc acccgatcag cccgtcggtc ccgatgccgg cgggcctgtc gggttcattg
     4141 cccaaagcgg gacgcatgcc gggttcttta cccaagccgc gcccgagcac gggatacgca
     4201 tcagcaagtc cgtatcgttc gggaacgcca ttgtcctcga cgcgagcgac tacctcgagt
     4261 acctcgccgt cgatgacgag acggagatca tcggcatgta catcgagggc gttcgcgatg
     4321 ggcgccggtt cttcgagacg cttcggcaga ctgcccggcg caagcccgtc gtcatctgga
     4381 agggcggcca aaccgaggct gggcagcgcg ccatctactc ccacactgca tcgctcgccg
     4441 cgcccatgcg catctgggat gcgatgacga aacaggtggg tgcggtctcg accaataatc
     4501 tggacgagac ggtcgatgtc atgaaggcgc tgctgcacgc aaaacccgcc acgcgaatgg
     4561 ccgcaggcct catcgcgatg acaggcggcc cctccgtcgg gatcacggac gcgttcgccc
     4621 gtgccggctt cgacgtcccg cgtctgcacg agggctccta cgaaaagctg tcgcagttct
     4681 tcaacgttat tggcggcagc taccgcaacc cattcgacgc cgggggaacg atcggtatgg
     4741 gcggccagcc gcagaatctc gacaagctgc tcgaaatcat cgacgaggac gacaacatcg
     4801 cggtggtcgc gatggacctc ggcttcggct tcgcgggccg ccgatggaag gagcggccgg
     4861 agatgctcga tgccatgctc gataccctcg ctgggttccg cgaccgttcg tcgaagccct
     4921 tggccctcat cctgcagccc gcgcacctgg aggacctggc cgtggagatg cgccgccggg
     4981 ttcaggagcg tggcttcgcg gtgttcgcca gctacgagcg ggccgcggac gcgatggcca
     5041 aggccatcgc gtaccatcgt tttcgggccg agctcgacta aacacgccca gcccagaagt
     5101 gcccatctaa ccatgccggc atccgcgctc cctcctctcg tggccgcgtt actccaaccg
     5161 cgcgcctacc cccatccggt cacggatgtg cgtctcgtgc aaacccacat ctcctacgtg
     5221 ctgatagccg gcgagcacgt gtacaagctc aagaagccgc tcgacctggg attcctcgat
     5281 ttccgaaccc tcgatgctcg ccgcatcgcg tgtgaacgcg aggttgcgct caacagccgt
     5341 ctctgcgccg aggcctacct cggcgtggtc cccattatcg agatgccgga aggacccgcc
     5401 atcggcgagt ccgcggaggg tcgagagccg ctagactggg cggtccacat gaggcggctg
     5461 ccggatgaga gcttcctcga ccgcaggctc ttggatgggc acgtaccggc gaacacggcc
     5521 gcgctcctgg cgcaacgcct cgtcgagttc catgaggctg cggcgacgaa cgacgaggtc
     5581 gcgcgctacg gctcggtgga gctcattgcc gcaaactggc aggagaactt cgaccaggtg
     5641 cggccctaca tcggccgcac gatcgcgcag gccgatttcg acagcctcgt cagctacgtg
     5701 gatcgcgtgc tgcgagagga tgccggtacg ttcgcacgtc gggccaagcg cgaccgcgtt
     5761 cgcgacgggc acggcgactt gcgctgcgat agcgtttcgc tcggcgacga cggctccctc
     5821 tgcatcatcg attgcatcga attcaacgat cggttccggt acgccgacgt cgcatcggac
     5881 ctcgcgttcc tcctcatgga tctcgaccgg cttggggcga gctggctcgc cgacgaggtg
     5941 ttgacccagt acctcgcgcg ttccttcgac gagtcactgc cgctcgtcct tcccttctat
     6001 cgctgctatc gcgccttcgt gcggggaaag gtgcatgggt tcgagatgga cgaggccgag
     6061 attcccgccg agcagcgtgc gagggccgag ctgacggctc gccgctgctt cgacctcgcc
//
LOCUS       DATMBB010000276         3219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_403658, whole genome shotgun sequence.
ACCESSION   DATMBB010000276 DATMBB010000000
VERSION     DATMBB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403658"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /gene="carB"
                     /locus_tag="VNL92_05960"
     CDS             <1..1907
                     /gene="carB"
                     /locus_tag="VNL92_05960"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWO94291.1"
                     /translation="VLARSTGIDPWFLDRLFAIVAMERRLLSEPLTRDLLREAKRMGF
                     SDADIGQLADRLPDQVRQDRQRWNIRPVYKMVDTCAAEFEAATPYFYSTYEEENEATT
                     LDGQRALVIGSGPIRIGQGIEFDYCSVHAATALREAGYRAILANSNPETMSTDFDTSD
                     RLYFEPLDEEAVRDILDNEGAGGESPATVVQFGGQTAINLAAPLHRAGLPIIGSSAEA
                     IDLAEDRRRFEDFLQRLGIPQPPGTTVVDVEGALRAAQQLGYPVLVRPSYVLGGRAME
                     IVQNAGELVRYMAHAIDAAPGKPILIDKYLEGKEVEVDAICDGESVLVPGIMEHIERA
                     GVHSGDSMAVYPAINLTQDEVTTVIDYTRRIGLAMGVCGLMNVQYVIRPEPDRPGPGV
                     YVLEVNPRSSRTVPFLSKVTGVPMVQAAVKVMLGQSLREQGYPDGLWPKQPLVAVKAP
                     VFSMAKLIGVDTYLGPEMKSTGEVMGIDRTFEAALAKALAASDLMLDRGASMLLSIAD
                     RDKPESIPLIRRLHEAGCRLYATEGTAEMIRGLGVPVEMVTKRLDQGHPNMVDVIREG
                     TVDGVVNTAESARTGTLRDGFHIRRAASERRIPCFTSLDTARAAVEALTAGSPVLNVL
                     PIGAYRSRSR"
     gene            1907..2695
                     /locus_tag="VNL92_05965"
     CDS             1907..2695
                     /locus_tag="VNL92_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWO94292.1"
                     /translation="MGAGHPHVQDAFVIETAEIAENLWLTWLGGVEAHRSARPGQFFM
                     FMPGFSDDPLLPRPMSYHRFRDRDGAFEFAVLYSIVGRGTGWLAERRPGDEVPVLGPL
                     GNGFDVRPDSGNLLCVAGGVGVAPFAALADEAIRHGRSVLLAMGARTASMLLPAPLVP
                     AEVEIAVATDDGTAGHRGPVSELFERHLAWCDQAFACGPTPMFHALAAIRRRSRWRKP
                     VQALLEERMACGFGACYSCAVRPRRGGVKLVCRDGPQFDLLEMF"
     gene            2695..>3219
                     /locus_tag="VNL92_05970"
     CDS             2695..>3219
                     /locus_tag="VNL92_05970"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HWO94293.1"
                     /translation="MNQRRPSPLVKWAGGKSSALAPILASAGTLPGHDGTYFEPFLGG
                     AAAFLAIAPSRAVLSDTNAELIAMYEAVRDEPEALMRELDALQPYVADREYYYAERAR
                     APQAMSRARAAARFIYLNKTCYNGLYRVNRRGQFNVPFGRYTTPPRLYDRENLLAVSA
                     ALGAAELVCADYRVA"
BASE COUNT          523 a         1106 c         1058 g          532 t
ORIGIN      
        1 tcgtcctcgc gcgcagcacc gggatcgatc cgtggttcct cgatcggctt ttcgctatcg
       61 tcgcgatgga gcgccgcctc ctctcggagc cgctgacgcg cgaccttctc cgcgaagcga
      121 agcgcatggg cttttccgac gcggacatcg gtcagctggc tgaccgtcta ccggaccagg
      181 tacgacagga tcgccaacgc tggaacatcc gtcccgtcta caagatggtc gatacgtgcg
      241 ctgcggaatt cgaagccgcg accccatact tctacagcac ttacgaggaa gagaacgagg
      301 cgacaacgtt ggacggccaa cgcgccctcg tcatcgggtc gggcccaatt cgcatcggcc
      361 aagggatcga gttcgactac tgcagcgtcc acgccgcgac cgcccttcgc gaggccggct
      421 atcgcgccat cttggcgaat tccaaccccg agacgatgtc caccgacttc gacacatcgg
      481 accggctcta cttcgagcct ctggacgagg aggccgtgcg cgacatcctg gacaatgagg
      541 gcgccggagg cgaatcgccc gcgaccgtcg tccagttcgg cggccagacc gccatcaacc
      601 ttgcggcgcc gctccatcga gccggcctgc ccatcatcgg cagcagcgcg gaggcgatcg
      661 acctcgcgga ggaccgcagg cggttcgagg atttcctcca acgcctgggc atcccgcagc
      721 cgcccggcac tacggtcgtc gatgtcgagg gagccctgag ggcggcccag cagctcggct
      781 atccggtgct cgttcgcccc agttacgtgc ttgggggccg cgcgatggaa atcgtgcaga
      841 acgcgggcga gctcgtccgc tacatggctc acgcgatcga cgccgccccg ggcaagccga
      901 tactcatcga caagtacctg gagggcaagg aagtcgaggt cgatgcgatt tgcgacggag
      961 aatccgtgct cgtgcccggc atcatggagc acatcgagcg ggccggcgtc cattccggcg
     1021 actcgatggc tgtctatcct gcgatcaacc tgacgcagga cgaggtcacc accgtcatcg
     1081 actacacgcg ccgcataggg ttggcaatgg gcgtttgcgg gctcatgaac gtgcagtacg
     1141 tcatccggcc ggagcccgac cggccgggcc ccggcgtgta cgttctcgag gtgaaccccc
     1201 gttcgagccg caccgtcccc ttcctctcga aggtgacggg cgtgccgatg gtacaggcgg
     1261 cggtcaaggt gatgctgggc cagtcgctcc gcgaacaagg ctacccagac gggctttggc
     1321 cgaagcagcc actcgtcgcc gtcaaggccc cggtcttctc gatggccaag ctcatcggcg
     1381 tcgatacgta ccttggcccg gagatgaaat cgaccggcga ggtcatgggc atcgaccgaa
     1441 cgttcgaggc ggccctcgcg aaggctctcg cagcctccga cctgatgttg gaccggggcg
     1501 cctcgatgct cctctccatc gcagaccgag acaagccgga gtcgatcccg ctcatccgcc
     1561 ggctccacga ggccggctgc cggctctacg ccaccgaggg cacggccgag atgattcgcg
     1621 ggctcggcgt gcccgtggag atggtgacga agcggctgga ccagggccat ccgaacatgg
     1681 tcgacgtcat tcgcgagggc acagtcgatg gcgtggtgaa cacggccgaa agcgctcgca
     1741 ccggcacgct gagggatggc ttccacattc ggcgggcggc aagcgagcgt cgcatcccct
     1801 gcttcacctc gctcgatacg gcgcgggccg ccgtcgaggc cctgacagcc ggatcgccgg
     1861 tgctgaacgt gctccccatc ggcgcctacc gatcgcggtc gaggtgatgg gagccggaca
     1921 tccgcacgtt caagacgcgt tcgtcatcga gaccgccgag atcgcggaga acctctggct
     1981 cacgtggctg ggcggagtag aagcgcatcg cagcgcccgt cccggacagt tcttcatgtt
     2041 catgccgggc ttcagcgacg atccgctgct cccgcggccg atgagctacc accggttccg
     2101 cgaccgcgat ggcgccttcg agtttgccgt cctgtacagc atcgtgggac gcggcacagg
     2161 ttggctcgct gagcgacgcc ccggcgacga agtgcccgtc ctcggtcccc tcggaaacgg
     2221 cttcgacgtg cgaccggact cgggaaacct cctctgcgtg gcgggaggcg tcggcgtcgc
     2281 gccgttcgcg gccctcgccg acgaagcgat ccgccacggc cgcagtgtcc tcctcgccat
     2341 gggagcccgc actgcgtcga tgctgctgcc cgcgccgctc gtcccggccg aggtcgagat
     2401 cgccgtggcg accgacgatg gcaccgccgg gcatcgtggt cccgtcagcg agctcttcga
     2461 gcggcacctc gcgtggtgcg accaagcgtt cgcgtgcgga cctacgccca tgttccacgc
     2521 cctggccgcg atccgccgtc gctctcgatg gcggaaacct gtgcaagcgc tcctggagga
     2581 gcggatggcg tgtggcttcg gcgcttgcta cagttgcgcg gtgaggcccc gacggggtgg
     2641 cgtgaagctg gtctgccgcg atgggccgca gttcgacctt ctcgagatgt tctgatgaac
     2701 cagaggcgac cctcaccact cgtaaagtgg gcgggcggga agagcagcgc tctcgcgccc
     2761 atcctcgcct ccgcgggcac ccttcccggg cacgacggaa cctacttcga gccgttcctc
     2821 ggtggggcgg ctgcgtttct cgccatcgcc ccatcgcgtg cggttctctc cgatacgaac
     2881 gcggagctca tcgccatgta cgaggcggtt cgcgacgagc cggaggcgct gatgcgagag
     2941 ctcgacgcgc tgcagcccta cgtcgccgac cgggagtact attacgcgga gcgggcccga
     3001 gcgccgcagg cgatgagccg ggcccgggct gctgcccgtt tcatctacct caacaagacg
     3061 tgctacaacg gtctgtatcg cgtgaaccgg cggggacagt tcaacgttcc cttcgggcgg
     3121 tacactacgc caccgcgcct ctacgatcgg gagaacctgc tggcggtcag cgctgccctc
     3181 ggcgccgcgg agctggtgtg cgccgactat cgggtcgcc
//
LOCUS       DATMBB010000277         3872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_242041, whole genome shotgun sequence.
ACCESSION   DATMBB010000277 DATMBB010000000
VERSION     DATMBB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242041"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1787)
                     /locus_tag="VNL92_05975"
     CDS             complement(96..1787)
                     /locus_tag="VNL92_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWO94294.1"
                     /translation="MSTRETSGAPESAPRPKGMPSHLSVLCRMLRSPEENRRCAAALV
                     LAELAPRETPVVEALGEVLADENRVLTNYALDALERIGSRAALPYLLPLLERDDQTRL
                     RAQRAIAALGEPAILDVCRELAQAPRERRRPLIQLLVDLDGKGALATVADALLLEDPE
                     LVSEVVTRYARRWSSLSPEARAERREVLLKLLDRQMPHGRHTAQVLALQLLGALADPS
                     ARKTVLPYSRPKQPPAVRRAALLALRRLPPPKGKEETAFAELAALLTEADYEHVVAPA
                     TEALRALPTPSAATSVLKPLLDSPHHTVRRFAAERLGEIDDPAAATALLKVLRGDDAA
                     LRETVQDALRKSPSALAGLVRALRSSESPEDAWTLARLVAARAAELTPALVKAVSEEA
                     LHAVERGDARAEAILHALRAADPARHREVLLQHGQRLLKKGKTEDAARILAPLDRGEA
                     DAEARFALAVALLKTSPKDLNRKVRLGDRSLSLFAGLVDEPDFPLANRLFADKNVSAE
                     DVFYVAFHLAEGDEAQREVGIALLEKLVKSEGRSKLGKIAKNKLALARKRQDAEQ"
     gene            complement(1849..2736)
                     /locus_tag="VNL92_05980"
     CDS             complement(1849..2736)
                     /locus_tag="VNL92_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94295.1"
                     /translation="MASLARFSEWSRKAALLGALLVPASAAAALAVHAWRAAEVPDLG
                     DWTRAAAAVRSQFRAGDLIAFAPGWAQEGRGLFRGLDVLPQERWSRDDIARARRLFVV
                     SSFDGRTPAWLSDVGREEAREDVGGIVVRRFELLFPRALTDFRALGPVEVSLDATPCT
                     RSGERWDCTQSGRFPWRWVGPQFMQIGGRARSCIYAHPTTRARLRIAFPPATLGSRLL
                     IGHGLSDAVAGRGASVTLGVSVAGEPISSLEHGQERGWRADSVDTSRWRGRSERVEFT
                     VTTDNDGARHFCFTAEALE"
     gene            complement(2745..>3872)
                     /locus_tag="VNL92_05985"
     CDS             complement(2745..>3872)
                     /locus_tag="VNL92_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94296.1"
                     /translation="LPCDQSYNSRLRKEDWDEPADYARLISYFRYGQPSDPLYVRAGQ
                     LHGATFGHGTVLNAYYNAINLDLYKLGVQINVNTDYGGVQTVMDNLFQPNLFGARVYV
                     RPMSFFDKASYANNLAIGMTVLADAFAPVGTPPPTTSRSGHEPPLPTEAAVVLGVDVE
                     FAVLRTKMLDVIPYTDFNHILGARGGWHLGVLTKVRPLDKLGLNFRLEYRYLSTQYVP
                     GYFDSLYDIQRYTFPLGCTTTKFDYVRERNRQQACLVGLGGSGFYGEGVFSLFDLLTI
                     LITYEDTVGPNNSNLLLSVQLPAFDAVKFAAYYYKRYFDGLSNAFSLDNAVLVGEARI
                     RMYSFMYFIARYARSWTLSADGTRFDSTDDFSVGIGFQARF"
BASE COUNT          674 a         1263 c         1306 g          629 t
ORIGIN      
        1 tgtcctggct cgtggcggcc aggagcgcga agtagccgac tcccagagcg gtggcgaccg
       61 cgaggtcgga tctcgtcagt cgcctgccca tgaacctact gttcggcgtc ctgtcgcttg
      121 cgcgcgaggg cgagcttgtt cttcgcgatc ttccccagct tggaacgccc ctcgctcttt
      181 acgagcttct ccagcaaggc gatgcccact tcgcgctggg cttcgtcacc ttcagcgaga
      241 tggaacgcga cgtagaagac gtcctctgcc gagacgttct tgtccgcgaa gagccggttc
      301 gccaagggga agtctggctc gtctacgagt ccggcgaaga gcgataggct gcggtccccg
      361 agccgaacct tgcgattcaa gtccttgggc gatgtcttga ggagcgccac ggcaagggcg
      421 aagcgcgcct ccgcgtccgc ttcgccacgg tccaaaggcg ccagaattcg cgccgcatcc
      481 tccgtctttc ctttcttgag cagtcgctgt ccgtgctgca ggagcacctc ccggtggcgc
      541 gccgggtccg cagccctgag cgcgtgcaga atggcttccg cacgggcgtc ccccctctcc
      601 accgcgtgaa gcgcttcctc ggacacggcc ttgacgagcg ctggggtcag ttcagccgct
      661 ctggcggcga cgagccgcgc caacgtccag gcgtcttccg gcgactcgct gctcctcagc
      721 gctcgcacga gccccgcaag cgcggagggg ctcttgcgca gggcatcctg cacggtttcg
      781 cgtagcgccg cgtcgtcgcc ccggagaacc ttcaggagcg cagttgcagc cgcaggatcg
      841 tcgatctcgc cgagccgctc ggcggcgaac cggcggaccg tgtgatgggg gctgtccaga
      901 agcggcttca gcacactggt ggcggccgaa ggggtcggca gggctcgaag cgcttccgtc
      961 gctggcgcga cgacgtgctc gtagtcggcc tcggtcaaca acgccgcaag ctccgcaaag
     1021 gcggtctctt ccttgccctt cggcggcggc agacggcgga gcgccaacag ggcggcgcgg
     1081 cggactgccg gcggctgctt cgggcgcgag taggggagaa ccgtcttccg cgcggaaggg
     1141 tcggccagcg ctccgagaag ctgcagcgcc agcacctggg ctgtgtggcg gccgtgaggc
     1201 atctggcgat caaggagctt cagcagcact tcgcggcgct cggcgcgcgc ctcgggcgac
     1261 aaggagctcc accgccgcgc atagcgcgtc accacttccg acacgagctc gggatcttcg
     1321 aggagcaacg catcggcgac agtcgccagc gcgccctttc cgtcgagatc gacgagcagc
     1381 tgaatcagag gtctccggcg ctcacgaggc gcctgggcca gctcgcgaca gacgtcgagg
     1441 atcgccggct cgccgagcgc cgcgatcgcg cgttgtgctc tgagccgcgt ctggtcatcg
     1501 cgctcgagca gcggaaggag atacggaaga gcggcgcgcg atccaatacg ttcgagcgcg
     1561 tcgagcgcgt agttcgtgag gacccggttc tcgtctgcga gcacttctcc caaggcctcg
     1621 acgaccggtg tctcgcgtgg ggcgagctcc gcgagcacga gcgcggcggc acaacgccgg
     1681 ttctcctccg gagagcgcag catgcggcac agcaccgaga ggtgggacgg catccccttg
     1741 ggacgaggcg ccgactccgg cgcgcccgac gtttctctcg tgctcatcgg tccgtatccc
     1801 tccccgctcc gcgggacgcc gggcgcaccg cgctttgatt ggctggcgtc attcgagcgc
     1861 ctcggccgtg aagcagaagt gccgcgcgcc gtcgttgtcc gtagtcaccg tgaactctac
     1921 gcgctccgat cgaccgcgcc agcgcgacgt gtcgacagaa tccgcgcgcc atcctcgctc
     1981 ctgtccgtgc tcgagagagc tgatgggctc acccgcgaca gagaccccga gcgtgaccga
     2041 ggcgccgcgc ccagcgaccg catcggagag gccgtggccg atgagcagtc ggctgccgag
     2101 cgtcgcgggc ggaaatgcaa tgcggaggcg cgcacgcgtc gtcggatgcg cgtagatgca
     2161 cgaccgggct cttcctccaa tctgcatgaa ctgtgggccg acccagcgcc acgggaaccg
     2221 tccgctctgg gtgcaatccc accgttcacc ggacctggtg catggagttg cgtcgaggga
     2281 cacctcaacc ggtccaagcg cgcggaagtc cgttaaggcg cgggggaaca gcagctcgaa
     2341 ccgccggacg acaatcccgc caacgtcctc acgagcctct tcccggccca cgtctgagag
     2401 ccacgccggc gtccgtccgt cgaacgagct cacgacgaac agccgccgcg cacgagcgat
     2461 gtcgtcccgg gaccagcgct cctgcgggag aacgtcgaga ccgcgaaaga gcccgcgccc
     2521 ttcctgtgcc caaccgggag cgaaggcgat caggtcaccc gcgcggaact gagatcgtac
     2581 agccgctgca gcgcgcgtcc agtcgcccag atcgggcacc tccgcagcgc gccacgcgtg
     2641 aacggcgagg gccgctgccg cggacgcggg cacgagcagc gcgccgagga gcgccgcctt
     2701 tcgcgaccat tctgaaaacc gtgccaggga cgccatcccg ctcctcagaa acgagcctgg
     2761 aaaccgatac caaccgagaa gtcgtccgtc gagtcaaatc tcgtgccgtc ggccgacagg
     2821 gtccacgagc gggcgtaacg ggcgatgaag tacatgaacg agtacatacg aatccgcgcc
     2881 tcgccaacga gtaccgcatt gtcgaggctg aaagcgttgg acaggccgtc gaagtaccgc
     2941 ttgtagtaat aagcggcgaa cttcacggcg tcgaaggcag gcaactgcac gctgaggagg
     3001 aggttcgagt tgttcggccc gaccgtgtcc tcataggtga tgaggatcgt caggaggtcg
     3061 aacagcgaga acaccccctc gccgtagaac ccgctgccgc cgaggcccac gaggcatgcc
     3121 tgctggcggt tgcgctcccg gacatagtcg aacttcgtcg tcgtgcagcc caatgggaat
     3181 gtgtaccgct ggatgtcgta aagtgaatcg aagtagcccg gcacgtactg ggtggagagg
     3241 taacgatact ccaggcggaa gttcagcccg agcttgtcga gggggcgaac cttcgtgagc
     3301 actcccaagt gccagccgcc gcgggcaccg aggatgtggt tgaagtcggt gtagggaatg
     3361 acgtcgagca tcttcgtccg caggacggcg aactcgacat cgacgccgag cacgacggcg
     3421 gcctcggttg ggagcggagg ttcgtgtccg ctacgcgacg tagttggcgg cggcgtgccg
     3481 acaggggcaa aagcgtcggc gagcaccgtc atgcctattg ccaagttgtt ggcgtaagat
     3541 gctttgtcga agaacgacat cggccgaacg tagacccgag ctccgaacaa gttcggttgg
     3601 aagaggttgt ccatcacggt ctggacgccg ccgtagtcgg tgttgacgtt gatctgcacc
     3661 ccgagcttgt agaggtcgag attgatggcg ttgtagtaag cgttgaggac cgtcccgtgg
     3721 ccgaatgtcg ccccgtggag ctgtcccgca cgtacataga gtggatcgga gggttgtccg
     3781 taccggaagt agctgatgag acgggcgtag tcagctggct cgtcccagtc ctccttgcga
     3841 agccggctgt tgtagctctg atcgcaaggt ag
//
LOCUS       DATMBB010000278         5422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_65888, whole genome shotgun sequence.
ACCESSION   DATMBB010000278 DATMBB010000000
VERSION     DATMBB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65888"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VNL92_05990"
     CDS             complement(<1..561)
                     /locus_tag="VNL92_05990"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94297.1"
                     /translation="MIRILYGPDTYSRTIALNRIRSELNADTADEVRFDASVGMEQLL
                     AALHTLPFLATRRVVIVDGLLARAQPPRRGASRTRARGSTRRDTSGLFWAPLADAAPT
                     LPPSTELILVDDAIDSDNPLLTALTPYAEVRQFRPLAQRDVPGWITARARDIGVRLAP
                     DAVRLLADLCGNNLWAAESELEKLHLY"
     gene            633..1571
                     /locus_tag="VNL92_05995"
     CDS             633..1571
                     /locus_tag="VNL92_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWO94298.1"
                     /translation="MEYRNLGKSGLQVSVLGLGTNNFGMRSDRDTSIAVVNRALDSGI
                     TFIDTSDSYGGQGRSEEYIGEALKGRRQEAVLATKFSSPMGEGPYMSGGSRRYVMRAV
                     EASLRRLQTDYIDLYQFHWPDPRTPIEETLRALDDLVRRGDVRYIGCSNFAAWQVVEA
                     AWTARTEHLTPFISAQNLYNLTDRGVERELLPACRACGLGFIPYFPLASGLLTGKYRR
                     GEPPPEGARLSAGPMASRFLSDRNFDLVERLESVASASGHTVGELAVAWLAAQPAIGS
                     VIVGVRSPEQLDAHLQAVEWKMTPEELQAIDEASRG"
     gene            1665..2429
                     /locus_tag="VNL92_06000"
     CDS             1665..2429
                     /locus_tag="VNL92_06000"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWO94299.1"
                     /translation="MHAHEVVAVGLDGQRETIVEVPNQPSGLGWLPDGTMLVVSMVDR
                     KLLRMGAKGLTEVADLSSLAPFHCNDMVVDAQGRAYIGNFGFDIYSGGAAEPRTTTLV
                     MVTPDGEARAVAEDMAFPNGSVITPDGRTLIVAETMARRLTAFDIRPDGSLENRRVWA
                     QLEVPPDGICLDADNCIWVAVPTRPGGFVRVAEGGKVLDRIDLEDRGGYACMLGGPDR
                     RTLLLLEAFDGNPANIARGNGRLRTVEVETGGAGLP"
     gene            2468..3658
                     /locus_tag="VNL92_06005"
     CDS             2468..3658
                     /locus_tag="VNL92_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94300.1"
                     /translation="MPALEGMRVLDMTQYEAGTSCTQLLAWLGADVVKLEPPGRGDPG
                     RGVGPHGSDVPSQYFLNYNSNKRSVVIDLAKEEGRDLLLDMAPHYDVFVENYGPGVIE
                     RLGLTYDEMRQRNPSIIYGRIKGFGLQGPYADYKCYDWVAQAAAGAFSVTGDRDGPPT
                     MPGPTIADSGAGVQMALAITAAYVQKLRTGEGQFIEVSMQEAVTLFMRTLGLAEWGKA
                     AAPRDGSRRGPPTDVYPCKPGGPNDYLFIMTVTTRMWDTLCAAIDRTDLLTDPRFENP
                     WARVQHGDDLHAEISKWTRERTKFEAMHELARAGVPCSAVLDTYDVFVDPHLQARAFI
                     RTVPHPTAGDVLLMGSPLRMSGSEVSIQAAPLLGEHTNDVLHTDLGLTDAQIAELRRA
                     GAVG"
     gene            complement(3683..3973)
                     /locus_tag="VNL92_06010"
     CDS             complement(3683..3973)
                     /locus_tag="VNL92_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWO94301.1"
                     /translation="MAGDIGEDIASLQNRLRRLEGQLRGIQRMLETGRHCDDVLTQIM
                     AVRSAVDQVGLMMLDHHLEHCVLPGTPVDEVRLGELRRAMRMWMRFAPVGDG"
     gene            4114..4440
                     /gene="trxA"
                     /locus_tag="VNL92_06015"
     CDS             4114..4440
                     /gene="trxA"
                     /locus_tag="VNL92_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWO94302.1"
                     /translation="MAKPTDVTDSDFDTAVLKAERPVLVDFWAPWCGPCRMVAPIVEE
                     LADEYEGKVEFRKLNTDDNPATAGRYGIRSIPTLLVFKDGEPVGQIVGFRPKSDLKKR
                     LDAALS"
     gene            4546..>5422
                     /gene="trxB"
                     /locus_tag="VNL92_06020"
     CDS             4546..>5422
                     /gene="trxB"
                     /locus_tag="VNL92_06020"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWO94303.1"
                     /translation="MTEREYDVAIIGAGPAGLAAALYAARARRRTVLFERRGPGGQIA
                     TTDIVENYPGFPEGVDGFELGQRMHQQAEKYGLETEYLDITSIARDEDGRYVLHSDDG
                     EFVAKAVILTGGADYNHLNIPNEERLTGKGVSYCATCDAAFFRDATVAVAGGGDSAME
                     EALFLTRYATKVYVIHRREELRASRIMQERAFAEPKIEFIWNTVVDEVVGEDKVQAIR
                     IRNLLTGETRDLPVDGFFVAIGQTPNSDVVRGLVPLDEGGHVPVNEWMETELPGLYAA
                     GDLRWRSARQVVSAAG"
BASE COUNT          949 a         1677 c         1895 g          901 t
ORIGIN      
        1 gtacaggtgc agcttctcga gttccgattc cgcggcccaa aggttgtttc cgcacaggtc
       61 ggcgaggagc cgcacagcgt caggcgcgag ccgcacgccg atatccctcg cgcgagccgt
      121 gatccatcct ggaacgtcgc gttgggcaag ggggcgaaat tggcgaacct cggcgtacgg
      181 tgtgagggcg gtcagaagcg gattgtcgct atcgatggcg tcatccacca ggatgagctc
      241 cgtgcttggc ggcagggtgg gggcggcgtc ggcaagcggt gcccaaaaca acccggatgt
      301 atcgcgccga gtcgacccgc gcgcgcgagt ccgtgaggcg ccgcgccggg gcggctgcgc
      361 ccgagccagc aggccatcga cgatgacgac gcgccgcgtg gccaggaagg ggagggtgtg
      421 gagcgcggcg aggagctgct ccatgccgac ggacgcgtcg aaccgcacct cgtcggcggt
      481 gtccgcattg agctcggatc ggattcgatt gagcgcgatg gttcgcgagt aggtgtctgg
      541 cccatacagg atgcggatca cgttcgcagt ataccggcgg cctcgggtcg atcgggtata
      601 ctgcgtgcct catcgcaaga caggaggtgg acgtggagta taggaatctc ggcaagtctg
      661 gattgcaagt ctccgtcctc ggcctgggca cgaacaactt cggtatgcgg agcgaccgtg
      721 atacgtcgat cgccgtcgtc aatcgggcct tggattccgg aatcacgttc atcgatacgt
      781 ccgacagcta tggagggcaa ggccggtcgg aggagtacat cggcgaagcg ctgaagggac
      841 gccgtcagga ggccgtgctg gccacgaagt tctcgtcccc gatgggcgaa ggtccgtaca
      901 tgagcggagg atcgcgacgc tacgtgatgc gggccgtcga ggcgagtttg cgccggctgc
      961 agaccgacta catcgacctg taccagtttc attggcccga cccgcgaacg ccaatcgagg
     1021 agacgctcag ggcgctcgac gaccttgttc ggcggggcga tgtgcgctac atcggctgct
     1081 cgaatttcgc ggcatggcag gtcgtcgagg ctgcgtggac ggcgcgcacg gagcatctga
     1141 cgccgttcat cagcgcgcag aacctgtata accttaccga tcgcggcgtg gagcgcgagt
     1201 tgttgccggc ctgccgcgcc tgcggtcttg ggttcatccc gtactttccg ctcgccagcg
     1261 gactgctgac gggcaagtat cgacgaggcg agccgccgcc ggagggagcg cgcctctccg
     1321 ctgggccgat ggcctcgcgc ttcctcagcg atcgcaactt cgatttggtc gagcgtctgg
     1381 agtcggtcgc gtcggcgtcg ggacatacag tcggagagct cgcggtagca tggctcgcgg
     1441 cgcagccggc catcggcagc gtcatcgtag gcgtgaggtc gccggagcaa ctcgacgcgc
     1501 acctgcaggc agtggagtgg aaaatgacgc cggaggagct gcaggccatc gatgaggcga
     1561 gcagggggtg agatatgccg ctcggagagc cgaaggttct gctcgaaggt ctcgtctttc
     1621 cagaagggcc gcgctggcat ggtggacggt tttgattcag cgacatgcat gcgcacgagg
     1681 tggtggcggt ggggctggac ggacagcgcg agaccatcgt cgaggtgccg aaccaaccct
     1741 cggggctagg ctggctgccc gacggcacga tgctcgtcgt ttcgatggtg gaccgcaagc
     1801 tcttgcggat gggcgccaag ggcctcacgg aggtagcgga cctcagcagt ctcgctccat
     1861 tccactgtaa cgacatggtc gtcgatgcgc agggccgagc ctacatcggc aatttcgggt
     1921 tcgatattta cagcggaggc gcggccgagc cgcggaccac aaccctcgtg atggtgacgc
     1981 cggacggcga ggcgcgtgca gtggcggagg acatggcctt cccgaacgga tccgtcatca
     2041 cgcccgacgg tcggacgctc atcgtcgccg agacgatggc ccgccggctt acggccttcg
     2101 atatccggcc cgatggctcc ctcgaaaacc gccgcgtctg ggcgcagctc gaagttccgc
     2161 cggacggcat ctgcctggat gcggacaact gcatctgggt cgccgtgccg acgcgtcccg
     2221 gcggcttcgt gcgcgtggcg gaaggcggca aggtgctcga ccggatcgat ctcgaggatc
     2281 gcggcggcta cgcatgcatg ctgggcggcc cggaccggcg aaccttgctt ctcttggagg
     2341 cgttcgacgg caacccggcc aacatcgccc gcgggaacgg caggctgcgc accgtggagg
     2401 tcgagacagg gggcgccggt ctgccctgag cgaggcgact gagggctgta cgaaggaggg
     2461 ggaagagatg ccggcactcg aagggatgcg cgtgctcgat atgacacagt acgaggcggg
     2521 gacatcgtgc acgcagctgt tggcctggct cggcgccgac gtggtgaagc tggagccgcc
     2581 gggccggggc gaccccggcc gtggcgtggg cccgcacggt agcgacgtgc cttcccagta
     2641 cttcctgaac tacaacagca acaagcgcag cgtcgtcatc gacctcgcga aggaagaagg
     2701 gcgcgacctc ctgctcgaca tggcgcccca ctacgacgtg ttcgtcgaga actacgggcc
     2761 cggcgtcatc gagcggctcg ggctgacgta cgacgagatg cggcagcgga atccgagcat
     2821 catctatggt cgcatcaagg gttttgggct tcagggtccc tatgctgact acaagtgcta
     2881 cgactgggtc gcccaggccg cggccggcgc cttctccgtc accggcgatc gggatggacc
     2941 accaaccatg ccgggcccga ccatcgccga ttcgggggcg ggggtccaga tggcgctggc
     3001 gattacggcc gcgtacgtgc agaagttgcg cacgggcgag gggcagttca tcgaagtatc
     3061 gatgcaggag gcggtcacgc tgttcatgcg tacgctgggc ctggcggagt gggggaaggc
     3121 cgctgctcct cgcgacggga gccgtcgcgg cccgccgacc gacgtctatc cctgcaagcc
     3181 cggcggaccg aacgactatc tcttcatcat gacggtgacc actcggatgt gggacacgct
     3241 gtgcgccgcc atcgaccgaa ccgatctgct gaccgatccg cggttcgaga acccatgggc
     3301 gcgggtgcag cacggcgatg atctccacgc ggagatatca aagtggacgc gcgagcgcac
     3361 caaattcgag gcgatgcacg agctcgcgcg ggcgggcgtt ccgtgtagcg ccgtcctcga
     3421 cacgtacgac gtcttcgtcg acccgcatct gcaggcacgc gcgttcatca ggacggtccc
     3481 ccacccgacg gccggcgatg tgctcctcat gggatcgccg ctgcgcatgt caggttccga
     3541 agtgtcgatc caggctgccc cgctgctcgg cgagcacacc aacgacgtct tgcatacgga
     3601 ccttggcctg acggacgcac agatagccga gctccggcga gcgggtgcgg tcggctaggg
     3661 gacttgggcg ccggagcagc ccctagccat cgccgacggg ggcgaagcgc atccacatgc
     3721 gcatggcccg ccgcagctcc ccgagccgca cctcgtcgac cggcgttccg ggcaggacgc
     3781 aatgctcgag gtggtggtcc agcatcatga ggcccacctg gtcgacagcg ctgcgcacgg
     3841 ccatgatctg cgtcagcacg tcgtcgcagt gacggcctgt ctccagcatg cgctggatgc
     3901 cgcgcagctg gccttcgagc cggcggagtc tgttctggag cgaggcgatg tcttcgccga
     3961 tgtcgcccgc catggccccg ccctccgatt cgcgctccag gtgtctttcc gtccccaaac
     4021 gatcgccttt gacatacccc tatggggtat gctacagtct acccacgtcc catccatctg
     4081 tcaacgacag agcatgcacg aggaggaaca cgaatggcga agccaaccga cgtaaccgac
     4141 agcgatttcg acacggcagt cctgaaggcc gaacgccctg tcttggtcga tttctgggcg
     4201 ccgtggtgcg gcccctgccg gatggtcgcg ccgatcgtcg aggagctggc cgatgagtac
     4261 gaaggcaagg tcgagttccg caaactgaac acggacgaca atccggccac agctgggcgc
     4321 tacggcattc gtagcatccc cacgctgctc gtcttcaagg acggcgagcc ggttgggcag
     4381 atcgtggggt tccggcccaa gagcgacctg aagaagcgat tggacgctgc cttgtcttaa
     4441 gaaggcggca aggccgttgc ctgcgacagg gccgtccttc tcatggggga cggccctgct
     4501 agcgatgacc aacccgatgt gccaacatcg gaacgaggtg catggatgac ggaacgcgag
     4561 tacgatgtcg cgatcatcgg cgcaggtccc gcgggccttg cggcggcact ctacgccgcg
     4621 cgcgcccgcc gaaggacggt cctcttcgaa cgccgtgggc ccggtggaca gatcgccacg
     4681 acggacatcg tcgagaacta cccaggcttt ccggagggcg tcgatggctt cgagttgggg
     4741 cagcgcatgc atcagcaggc ggagaagtat ggcctcgaga cggagtacct cgacatcacg
     4801 tcgatcgcgc gcgacgaaga tgggcgctac gtgctccact ccgatgatgg cgagttcgtt
     4861 gcgaaggcgg tcatcctcac cggtggggct gactacaatc acctcaacat cccgaacgag
     4921 gagcggctga ccgggaaggg tgtctcgtat tgcgccacct gcgatgcggc cttcttccgc
     4981 gatgccacgg ttgccgtggc cggcgggggc gactcggcga tggaggaggc gctcttctta
     5041 acgcgttacg ccaccaaggt ctacgtcatc catcgccggg aggagttgcg ggccagccgc
     5101 atcatgcagg agcgcgcgtt cgccgagccg aagatcgagt tcatctggaa cacggtcgtc
     5161 gacgaggtgg tgggcgagga caaagtgcag gcgatcagga ttcgcaacct cctcacgggc
     5221 gagacgcgcg atctgccggt cgacggcttc ttcgtcgcca ttggccaaac gccgaatagc
     5281 gatgtcgtgc gaggcctggt gcccctcgac gaagggggcc acgttcccgt gaacgagtgg
     5341 atggagacgg agctccccgg tctctatgcg gccggcgacc tgcggtggcg ctctgccagg
     5401 caggtcgtga gtgcggcggg cg
//
LOCUS       DATMBB010000279         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_334071, whole genome shotgun sequence.
ACCESSION   DATMBB010000279 DATMBB010000000
VERSION     DATMBB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334071"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VNL92_06025"
     CDS             complement(<1..623)
                     /locus_tag="VNL92_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWO94304.1"
                     /translation="MSEIAELAGLNRATSYRFCQTLLSLGYLEEADERRLRPGLKAVS
                     LARAALSSHELPDLALPHLRELHDATGETVNMALLDGADVVYVSRLLSDDLLALRLYV
                     GSRLPAYASSLGRAILAHLPEDDVRALLDGQTFEAFTEHTIVDKARLMAELRRIRRRG
                     YAINNQELVLGISGVAAPVFGVSGRPVAAINLSLSRVPDRGEIESKLA"
     gene            908..1549
                     /locus_tag="VNL92_06030"
     CDS             908..1549
                     /locus_tag="VNL92_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94305.1"
                     /translation="MAPAVGPRQPSAVIHLAFLELERAVEAEFIRWYEGEHVPRMLAR
                     PGWRRMACYRCTDGYPLLSLYDLDSDVPRAPPVSEAPFRSGPFAARGIRTYHARTWRE
                     IHAAGSPSWAAQWINAITVDVDPAHAEDFSRWYNEIHVPEILACPGWVANRRYECIDG
                     EPRFLAIYDLKDPKSPFASCKWRSAVGWDEHREHIRGYHGFRVYERIFDSEDR"
     gene            2129..>2545
                     /locus_tag="VNL92_06035"
     CDS             2129..>2545
                     /locus_tag="VNL92_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94306.1"
                     /translation="MMQRLGVTRVLLALVSVGLLVLAATGGLGTSATTQSGKGSLQCK
                     NGVLTVGIAKAQSGLASFFDIAGTRGTKIAIDQINAAGGIKGCKIKTIEGDTKSDPAV
                     AAQVARSLLDKGAQILLVPDDFDLGIAAARVGQQAGV"
BASE COUNT          436 a          821 c          848 g          440 t
ORIGIN      
        1 gccagcttcg actcgatctc gcctcggtcg ggcacgcggc tcagcgagag gttgatggcc
       61 gctaccggac gccccgacac accgaagacc ggtgccgcca cccccgagat accgaggacg
      121 agctcctggt tattgatggc atacccacga cggcgtatgc gacgtagctc ggccataagg
      181 cgcgccttgt cgacgatcgt gtgctcggtg aaggcctcga acgtctggcc gtcgagcagt
      241 gcgcgcacgt cgtcttcggg caggtgggcg aggatggctc gcccgagcga cgaggcgtat
      301 gccggcagac ggctgccgac gtagagtcgg agtgctagaa gatcgtcgct gagcaggcgc
      361 gagacgtaga cgacgtccgc accgtccagc agtgccatgt tgacggtctc gccggtcgcg
      421 tcgtggagct cgcgaaggtg agggagcgcg agatccggaa gctcatgact gctgagcgcc
      481 gctcgcgcga ggctgaccgc cttgagcccc ggtcgcaggc gacgctcgtc cgcctcctcc
      541 aggtagccga ggctcaggag cgtctggcag aagcggtagg acgtcgctct gttcaacccc
      601 gcgagctcgg cgatctcgct catggtgaga gccggtcgac ggtcgctgaa gagctcgaga
      661 atcgtcaacg cccgacgaac cgattggatc ccatcggcgc gctctgcgtt cgctcggcgt
      721 ctcggctgtc ttgcgctcac gtgacaaaca ggcgatggcg tgcggtcgac ggatcgacgg
      781 tccgtcgctt gtccgatacg ctcacctgga gtaggtttct cacgatccgg tcagtatgcc
      841 gcactgcgat cacctccatc cgacttctct accgagtcgg gcccgacgct gacgcgggcg
      901 ccggagtatg gcccccgccg tcggtccccg ccagccgagc gcagtgatac acctcgcctt
      961 cctcgagctg gagcgagccg tcgaagcgga gttcatccgt tggtacgagg gcgagcacgt
     1021 cccccgaatg ctggcccgcc ccggctggag acgcatggcg tgctaccgct gcaccgacgg
     1081 ctatccgctg ctgtcgctct acgatctcga ctccgacgtt ccacgtgccc cacctgtctc
     1141 ggaggcgcct ttccgatccg ggccttttgc tgctcgaggg atccggacgt accacgcacg
     1201 cacgtggcgg gagatccacg ctgcagggag cccatcttgg gcggcgcagt ggatcaacgc
     1261 catcacagtg gacgtcgacc cggctcacgc cgaggacttc agtcgttggt acaacgagat
     1321 tcacgttcca gagatcctcg cgtgtccggg atgggtcgcc aaccgtcgct acgagtgcat
     1381 cgatggcgag ccgcgcttcc tcgcaatcta cgacctcaag gatcccaaga gcccgttcgc
     1441 gagctgcaag tggaggtcag cagtgggttg ggacgagcat cgcgagcaca tccgtgggta
     1501 ccacgggttc cgcgtctacg aacgtatctt cgactcggag gacaggtgag gcggatgtgc
     1561 ggctctagcg ggcccggacc tgaacggagt tgagcgacga ggtgacgcga tcaggctcga
     1621 gccgagccgg gccgcggccg gatcgcgacg ggtgatgtcg tcgccttccg ggctcggcat
     1681 ggaagcagcg ccctggctag cgcggcgggc gaaggtcgtg accgtcacgg ccgatgcagg
     1741 aggagaggcc cttccgccgg aagaccccgg gcggttcctt tcggctctac ctcctggctg
     1801 aagagggaat gccgatcatc gagaacgtat ggctggagga gctggtagcc cgcggggtga
     1861 gtgaagtggc gtttctcgcg tttccgccga agcttcttgg ggagcaccgt cgcccccgtg
     1921 cgcccgatcg ctctctcgct ttccgagcag tcagcgcgcg ctcgcagcca ctacgcacag
     1981 ctgcatgtgc ccgccatacg gatgggcgtt cgctattcga tggctcgtgg cccgatagct
     2041 cttgcttgac ccagcgtagg tattggaata cccttgagcc ttcgcccacg acgcgaaaac
     2101 ctgtccgcat cgtgaaggga ggagatcaat gatgcagcga ctcggtgtca cacgggtcct
     2161 gctcgcgctc gtatcggtag gcctgctcgt tcttgccgca accgggggcc tcggaacaag
     2221 tgcgaccacg cagtcaggca aaggctcgct tcagtgcaag aacggcgtcc tcactgtcgg
     2281 gatcgcgaag gcgcaaagcg gcctggcgtc cttcttcgac atcgccggga cgcgcggcac
     2341 gaagatcgcg atcgaccaga tcaacgcagc aggcggcatc aagggctgca agatcaagac
     2401 gatcgaaggc gacaccaaga gcgatcctgc cgttgcggcc caggtcgcgc ggtcgcttct
     2461 cgacaagggc gcgcagatcc tcctcgtgcc cgacgacttc gacctcggaa tcgcagcggc
     2521 tcgcgtcggt cagcaggcgg gcgtc
//
LOCUS       DATMBB010000280         2698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_170729, whole genome shotgun sequence.
ACCESSION   DATMBB010000280 DATMBB010000000
VERSION     DATMBB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170729"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VNL92_06040"
     CDS             complement(<1..555)
                     /locus_tag="VNL92_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HWO94307.1"
                     /translation="MFDALTEKLNNTFRRLGSQGTVSEQDLDEAMREVRLALLEADVN
                     FKVAKKFISDVREKAVGAKVLESLTPVQHIIGIVNEELVAILGGEPAPLTRAPKPPTI
                     IMFAGLKGSGKTTTAAKLALHLRREGLKPALVAADPYRVAGSVQLQTLGKQLDLPVFG
                     LNGETSLEEATARGVADATAAGSTA"
     gene            complement(637..1788)
                     /gene="mqnC"
                     /locus_tag="VNL92_06045"
     CDS             complement(637..1788)
                     /gene="mqnC"
                     /locus_tag="VNL92_06045"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HWO94308.1"
                     /translation="MLNQIREKVEAGERIEREEGLFLLREAHLLDLAPLAQTWRFRHN
                     PEPAVTFVVDTNLNYTNVCDAFCTFCAFYRTPADRDAYTYSVEEMMEKFEQSLEKGVT
                     RILLQGGLNDALPLEYYEELLRESQRRYPQIHCHFFSAPEVQKMSQVSGLSVREVLQR
                     LKAAGLKSLPGGGSEVLSDRVKQKVSRLFPKGSTQDWVEVHREAHRLGIATTATMMYG
                     HLEEDDDIIEHLEVVRQLQDETHGFLAFIPWSFKKAHTALDRRVKEEAGPNRYLRIMA
                     LSRIYLDNVQHIQASWFSEWKKVGQVALHFGGDDFGGTLFDENVMQEAGFYNRTTVDE
                     VVAIIREAGFIPVERGTLYEFVRSYGDEAAAELGSPTAVPASVAGGRRD"
     gene            complement(1790..2593)
                     /locus_tag="VNL92_06050"
     CDS             complement(1790..2593)
                     /locus_tag="VNL92_06050"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HWO94309.1"
                     /translation="MTINVGRMPYLNSELFYLRMDSPEFDLHPLVPTAMSRAAQRGEL
                     DAGPFSLADCFRLGSPGGGFERLGGFCIASRDAARSILLFSRVPWEELDGGRIAITDE
                     TATSVRLLKVVAAQKLHIAPTYVGPNEEADALLLIGDGALRARRGVEGKPWVYDLGSV
                     WHEWTGLPFVFALWMVRRSLPEADKERLLRALDSSLEEGLRDLPAAAERRKDLGMSAD
                     EVSEYLGGFAFRAGPAEEQAIGEFTRRLRELVPSGPDELRELQALGLET"
BASE COUNT          460 a          912 c          849 g          477 t
ORIGIN      
        1 cgcggtgctg cccgccgcgg tcgcgtccgc cacgcctcgc gccgtcgcct cttcgaggct
       61 ggtctcgccg ttgagtccaa acaccggcag gtccagttgc ttgccgaggg tctgcagttg
      121 cacgcttccg gccacgcgat aggggtcggc ggcgacaagg gccggcttca acccctcgcg
      181 ccgaagatgc agcgcgagct tggcggccgt cgtcgtcttg ccggagccct tgaggccggc
      241 gaacatgatg atcgttggcg gcttgggtgc ccgcgtcagc ggcgcaggct cgccgccgag
      301 aatcgccacg agctcctcgt tgacgatgcc gatgatgtgc tgcaccggcg tcaggctctc
      361 gagcactttc gccccgacgg ccttctcgcg cacatcggag atgaacttct tggctacctt
      421 gaagttgacg tcggcctcga gcaaggccag ccgaacctcg cgcatcgcct cgtcgaggtc
      481 ctgctccgag accgtgccct ggctgccaag gcgccggaag gtgttgttca gcttctcggt
      541 tagagcatcg aacatactaa gtgaattcta gctgccgctt tccaggccgg tcaaatgccc
      601 gaactgcagc gatggctcga agactgggaa tccgtattag tcgcgccgtc cgcctgcgac
      661 ggacgcgggg accgccgtcg ggctcccgag ctcggccgca gcctcatcgc cgtacgagcg
      721 cacgaactcg tacagcgtac cgcgctccac cggaatgaaa ccggcctccc ggatgatcgc
      781 cacgacctca tccaccgtag tgcggttgta gaacccggcc tcctgcatca cgttctcatc
      841 gaacagcgtg ccgccgaagt cgtccccacc gaagtgcagc gcaacctgtc ccaccttctt
      901 ccactccgag aaccacgatg cctgaatgtg ctgcacgttg tcgaggtaga tgcgcgagag
      961 ggccatgatt cgcagatagc ggttcggtcc ggcctcctcc ttcacgcggc ggtcgagcgc
     1021 cgtatgcgcc ttcttgaagc tccacggtat gaaggccaag aagccgtgcg tctcgtcctg
     1081 gagctgccgg acgacctcca gatgctcgat gatgtcgtcg tcctcctcca gatggccgta
     1141 catcatcgtc gccgtcgtgg cgataccgag ccggtgagcc tcgcggtgga cttcgaccca
     1201 atcctgagtg ctgcccttcg gaaagaggcg gctcaccttc tgcttcacac gatcggacag
     1261 gacctccgaa ccgccgccag gcagactctt gaggcctgcc gccttgagac gctggagcac
     1321 ctcgcgcacg gatagtcccg agacctggct catcttctgg acctcgggcg cggagaagaa
     1381 gtgacagtga atttgaggat agcgccgttg gctctcccgc agcagctcct cgtagtactc
     1441 cagcgggagc gcgtcgttca ggccaccctg cagaaggatg cgcgtcacac ccttctcgag
     1501 cgactgctcg aacttctcca tcatctcttc gacggagtac gtgtacgcgt cgcggtcggc
     1561 aggcgtacgg tagaaggcgc agaaggtgca gaacgcgtca cacacgttcg tgtagttgag
     1621 gttcgtgtcg accacgaagg tcaccgcggg ctcggggttg tgccggaacc tccaggtctg
     1681 tgccagcggc gcaagatcga ggagatgcgc ctcgcggagg aggaataggc cctcctcccg
     1741 ctcaatgcgc tcgcccgctt ccaccttctc gcgaatctgg ttcagcatgt tatgtctcca
     1801 atccgagcgc ttgcagctca cggagctcgt ccgggcccga cggcacgagc tcgcggaggc
     1861 gccgcgtgaa ttccccgatg gcctgctcct ccgccgggcc cgccctgaag gcgaaaccgc
     1921 cgaggtactc gctgacctcg tcggccgaca tgccgaggtc cttgcggcgc tccgccgcgg
     1981 cggggaggtc ccgtagccct tcctccaggc tcgagtccaa cgctcgcagt aggcgttcct
     2041 tgtccgcttc cggcaagcta cggcggacca tccagagggc aaagacgaac ggcagccccg
     2101 tccattcgtg ccataccgaa cccaggtcat agacccacgg cttcccttcg acgccgcggc
     2161 gagcgcgaag ggcgccatcg ccgatgagga ggagggcatc ggcctcctcg ttcggtccga
     2221 cgtacgttgg agcgatgtgt agcttctgag ccgcgaccac cttcagcagc cggacggacg
     2281 tcgccgtctc gtcggtaatg gcgatgcgac ccccgtcgag ctcctcccac gggacgcgcg
     2341 agaacagtag gatgctgcgg gcggcgtccc tcgaggcgat gcagaagccg ccgaggcgct
     2401 cgaagcctcc cccaggcgaa cccaatcgaa agcagtcggc gagcgagaag ggcccggcgt
     2461 ctagctcgcc gcgctgggcc gcccgcgaca tcgcggtcgg tacgagggga tgcaggtcga
     2521 actcgggact gtccatccgg agatagaaga gctcggagtt caggtatggc atccggccga
     2581 cgttaatggt catacactct cacgacctcg tagagcgcgt tgcgctccac ggggactttg
     2641 ccggcatcgc ggacgagccg caccagccgt tcgcgcgcga gaacgatggg gctcgtgg
//
LOCUS       DATMBB010000281         4943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_37202, whole genome shotgun sequence.
ACCESSION   DATMBB010000281 DATMBB010000000
VERSION     DATMBB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37202"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VNL92_06055"
     CDS             <1..483
                     /locus_tag="VNL92_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94310.1"
                     /translation="LLEACKRMDADANITQEPHPTVKEAKSFAEEVVHERLERRMAAL
                     EEENRAAYKRESEKLAAFYAHRQAVAEEKFEREQRRLERIEALPEDDEARRILPALRA
                     RAKDADEARARVRRDQERRLAFLRQRAHVSPEFRLLAVAAVETSRPGSLPPISAAGAP
                     "
     gene            480..695
                     /locus_tag="VNL92_06060"
     CDS             480..695
                     /locus_tag="VNL92_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94311.1"
                     /translation="MTRATQYRSSSRTSGPTSPALSAMTRDRPYRKGVPADESISELR
                     ACAGSQLDPQAVEAFIAAYLRRGLHAA"
     gene            1131..2876
                     /locus_tag="VNL92_06065"
     CDS             1131..2876
                     /locus_tag="VNL92_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO94312.1"
                     /translation="MTTAADMLVDTLQEWGVDTVFGLPGDGINGIMETLRQRQDKVRF
                     IQVRHEESAAFMACAYAKFTGRLGVCLATSGPGGLHLLTGLYDAKLDGQPVLAITGHH
                     YHDLIDTRAQQDVDLDRVFEDVAVYNTRVMGPAHVENVTNLACRTALSYRGVSHINFP
                     VDLQEHEGGERSKRNIAGHNQEVLGQSAQVPDSSALQSAAEVLNAGSKVVILAGRGSL
                     GAREELEQVAEVLGAPIVKALLGKAAVPDMSPYTTGGIGLLGTKPSMEAMEDCDTLLM
                     VGTSFPYIEFFPVPGKARAVQVDLDPARIGLRYPVEVGLVGDSKKSLQALLPLLERNE
                     DRGFLDRAQSGMKDWWELMEERGTRRDSPMKPQVVAWELGKRLAPDAIVASDSGTVTT
                     WWARQIPAQGDQMFSCSGNLATMACGLPYAVAAQIAYPERQVVAFVGDGAFSMLMAEF
                     ATAVKYNLPIKVVVVKNDYLGQIKWEQMVFLGNPEYGVDLQPIDFAKFAEACGGTGIR
                     IEDAERCGEQIEEALRTPGPVLVEAVVDPLEPPMPASVTREQAMNFALSLLRGQPDRG
                     QIAAGVLKNRVRELI"
     gene            2890..4005
                     /locus_tag="VNL92_06070"
     CDS             2890..4005
                     /locus_tag="VNL92_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWO94313.1"
                     /translation="MDDRDDGFVVVGVDVSAYTIPTDGLEADGTLEWDETTLVLVELT
                     SASGITGLGFSYTSAAAALVVRDVLANLAIGRSVHDLPSIHEEMVRAVRNIGRAGVVA
                     SAIAAVDVALWDLRARLHDLPLFRLLGAVRHSVPLYGSGGFTSYEERRLVQQLTEWAK
                     AGIAAVKMKVGLDGGKYPDEDLARVRSVRQALPDGVRLFVDANGAYETKLAIRQANRF
                     AEQNVSYFEEPVSSDHLSQMAAVRAAAPMPIAAGEYAYDEYYFRRMLESDAVDILQAD
                     ATRCLGISGFLQGGQLAHSFAKPFSAHTAPSIHAHAACAVPSLEHVEYFHDHVRIEGM
                     LFDGVLEPRGGLLYPDPKRPGLGLEFKRAEAERWRVI"
     gene            4074..>4943
                     /locus_tag="VNL92_06075"
     CDS             4074..>4943
                     /locus_tag="VNL92_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWO94314.1"
                     /translation="MSDYEGLRRDLETQIRGEVRFDDGSRALYATDASNYRQVPIGVV
                     IPRDTDDVIAAVDLCRKHGAPVLSRGGGTSLAGQCCNVAVVLDMSKYVNRIIEIDSEA
                     RTASVEPGLVLDELRSAAEQHHLTFAPDPSTHDHCTIGGMIGNNSCGVHSLMAGKTVD
                     NVEELEVLTYDGCRFTVGSTDERELDEMIAADGRKGEIYGRLRALRERYADQIRARFP
                     DIPRRVSGYNLDELLPESRFHVGRSLVGTEGTCVVVLRAKLRLVPSPRHRALLVLGFP
                     DVHVAADHVPDVLA"
BASE COUNT          915 a         1545 c         1597 g          886 t
ORIGIN      
        1 ctcctagagg cctgcaagcg catggacgcc gacgcaaaca taacgcagga gcctcatccg
       61 acggtgaaag aggctaagtc gttcgctgag gaagtcgtgc acgagcggct tgagagacgc
      121 atggccgccc tggaggaaga gaaccgggcg gcctacaagc gagagagcga gaagctggca
      181 gccttttacg cacaccggca agccgttgcc gaggagaagt tcgagcgtga gcagcgcaga
      241 ctcgagcgga ttgaggcgct gccggaagac gacgaggcaa ggcggatcct gcctgcgctt
      301 cgcgcgcgtg cgaaagatgc agacgaggct cgcgcgcgcg ttcggcggga tcaggagcgg
      361 cgcttggcgt tcctccgcca acgcgcgcac gtcagtcctg agttccggct gctagctgtg
      421 gcagcagtgg agacctcccg tccggggtcc ctgcccccca ttagtgcggc cggcgcccct
      481 tgacgagggc cacccagtac cggtcgtcga gccgtacctc gggcccgacg tcgcccgccc
      541 tctcggccat gacgcgcgac cggccgtacc gcaagggagt ccccgccgac gagtccatca
      601 gcgagctgcg cgcctgcgcc ggctcgcaac tcgacccgca agccgtggag gcgttcatcg
      661 ccgcctacct gcggcgcgga ctccacgcgg cgtagcacag gcccctgcgg gcgggggagc
      721 ctcaccacaa ggccgccgcc tcgcttgtca ggccgcaatc ccgtgcgagg atgctttcgt
      781 ttcagtccgt cacgtcaccc tctcaatcag cgtgcacctg ccagctggag gccccacggc
      841 aagcgctctc ggagcattgt taggcgttcg ttagggagga gcagaaaccg tcatcccggt
      901 gcgaggatga gccccctacg atgcgattgg aactgattcc accggagcct cagtacgaca
      961 gaagtgagac aaaaatgtgg tcccgcccgg ggcctcactc gtgaagtcag ccagggccgc
     1021 gttgccggcg ggcttgtgat ggcgaagcaa gccgtcgtga gggtgcctga gcgcgccggc
     1081 tatcccccga aggaggtcta tcagatgtca ctgggctggg aggaggagac atgacaaccg
     1141 ccgcggacat gctggtagat acgctacaag agtggggagt cgatacagtt ttcggcctgc
     1201 cgggagacgg catcaatggg atcatggaaa cgctacggca gcggcaggac aaggtccggt
     1261 tcattcaggt gcggcacgag gaatcggccg ccttcatggc ctgcgcctac gcaaagttca
     1321 ccggtcgcct cggcgtttgc ctggctacct ctgggcccgg cggtctccat ctcctcactg
     1381 gcctctacga tgccaagctg gatggccagc cggtgcttgc gatcacgggc caccactacc
     1441 atgacctgat agatactcgt gcccagcagg atgtcgacct cgatcgcgtg ttcgaagacg
     1501 tggcagtgta taacacgcgc gtaatgggcc cggcgcatgt cgagaacgtg acgaacctcg
     1561 cctgcaggac cgcgctgagc tatcgaggag tctcacatat aaactttccc gtcgaccttc
     1621 aggagcatga aggcggagag cgatcgaagc gaaatatcgc cggacacaat caggaggtcc
     1681 tggggcagag cgcccaggta cccgacagct ctgcgctgca atcagcggcc gaggtactca
     1741 acgcgggatc gaaggtggtc atcctggccg gtcggggctc gctcggggcg cgcgaggaac
     1801 tcgagcaggt cgccgaggtc ctaggcgcac cgatcgtaaa ggcactgctg gggaaggcgg
     1861 ccgtaccaga tatgagtccg tacacgacgg gcggcatcgg tctgctcggg acgaagccgt
     1921 cgatggaggc catggaggac tgcgacaccc tgcttatggt cggtacatcc tttccgtaca
     1981 tcgagttctt ccctgtgcct ggtaaggccc gcgccgtcca ggtcgatctc gacccggcgc
     2041 gcatcgggct gcgctacccc gtggaagtcg gactcgttgg cgacagtaag aagtcgctcc
     2101 aggccctgct gccactcttg gaacggaacg aagatcgcgg cttcctcgat cgtgctcaat
     2161 ctgggatgaa ggactggtgg gagctgatgg aggagcgggg gacgcgcagg gactcgccga
     2221 tgaagccgca ggtggtggcc tgggagctgg gcaagcggct ggcaccggat gcgattgtcg
     2281 cttcggacag tggcacggta acaacttggt gggcgagaca gatcccagct cagggggacc
     2341 aaatgttctc ctgttccggt aacctcgcta cgatggcctg cgggcttccc tatgccgtcg
     2401 ccgcccagat cgcctacccg gaacgtcagg tggtcgcctt tgtcggcgac ggcgccttct
     2461 cgatgctgat ggccgaattt gcaacggccg tgaagtacaa tctgccgatc aaggttgtgg
     2521 tcgtcaagaa cgactacctc ggccagatca aatgggagca gatggtcttc ctcggaaacc
     2581 ctgagtatgg cgtagatctg caaccaatcg atttcgcaaa gttcgccgag gcctgcggtg
     2641 gtacggggat taggatcgag gacgcggaac gatgtggcga gcagattgaa gaggcgctcc
     2701 gcacccccgg tcccgtgctg gtcgaggcgg tcgtcgaccc cctggaacca ccgatgcccg
     2761 cgagtgtcac ccgagagcag gcgatgaact tcgcgctatc gctcttgcgc ggtcagcccg
     2821 atcgcggcca gatagctgcc ggagtgctca agaaccgggt gcgagagctt atctagtcgg
     2881 cgcctacaca tggacgatcg agacgatgga ttcgtcgtcg ttggagtcga tgtcagcgcc
     2941 tacaccattc ccaccgatgg tctcgaggct gacgggacgt tggagtggga cgagactacg
     3001 ttggtacttg tcgagctcac cagcgcttcc ggaatcaccg gcctgggttt ctcttacacg
     3061 tcagcggccg ctgcactcgt cgtccgtgat gtcctcgcca accttgccat cggccgctcg
     3121 gtccacgacc tgccctcgat ccatgaggag atggtgaggg cggtccggaa cattggacgc
     3181 gcgggcgttg ttgcctccgc catcgccgcc gtggacgtgg cgctctggga ccttcgcgct
     3241 cgactgcacg acctgccgct cttccggctc ctcggtgccg ttcgacacag tgtccctctc
     3301 tacggcagcg gcggcttcac ctcctacgaa gaacgccgac tcgtacagca gctcacggaa
     3361 tgggccaaag cggggatcgc cgctgtcaag atgaaggtag gcctggacgg gggtaagtat
     3421 ccggatgagg atctggctcg cgtccgctcg gttcggcagg ccctccctga tggtgtccga
     3481 ctcttcgtag acgcgaacgg cgcttacgag acgaagctgg ccatccggca agcgaaccgg
     3541 ttcgccgagc agaatgtctc ctacttcgag gaacctgttt cgtccgacca tctttcgcag
     3601 atggcggctg tccgcgccgc ggcaccaatg ccaatcgcgg caggcgagta tgcgtacgac
     3661 gagtactact tccgacgaat gctagagtcg gatgcggtcg atatcctcca ggccgacgca
     3721 acccgatgcc tcgggataag tggcttcctc cagggcggtc agctcgccca cagcttcgcg
     3781 aagccttttt cggcccatac tgctccttcg attcacgcgc acgctgcctg cgccgtcccc
     3841 tcccttgagc acgtggagta cttccatgac cacgtccgga tcgaaggcat gctgttcgac
     3901 ggcgtccttg aacctcgcgg cgggctactt tatccggatc ctaaacgccc aggcctgggc
     3961 cttgagttca agcgcgccga agcggagcgc tggcgagtca tctgacgaaa gggctggtga
     4021 tgagggcatg gtgggacgaa caactcaaca atcgcatggg gagactagcg gcgatgagcg
     4081 attacgaggg gctgcgacgt gacctggaga cgcagattcg gggtgaggtg cggttcgacg
     4141 acggcagccg cgccctttac gcgaccgatg cttccaacta tcggcaggtg ccgatcggcg
     4201 tcgtcatccc ccgcgacact gatgacgtca ttgccgccgt cgacctctgc cgaaagcatg
     4261 gcgctcccgt gctctcgcgt ggcggaggaa cgagcctcgc tgggcagtgc tgcaacgtgg
     4321 ctgttgtgct tgacatgtcg aagtacgtca accggatcat cgagatcgat tcggaagcgc
     4381 ggacggccag cgtcgagcct ggactggtgc tcgacgagct ccgttccgcg gcggaacagc
     4441 atcacctgac ctttgcgccc gatccctcaa cccatgacca ctgcaccatc ggcggcatga
     4501 tcggcaacaa ttcctgtggc gtccattcac ttatggcggg aaagaccgtc gacaacgttg
     4561 aagagctgga agttctcacg tatgacggtt gccgtttcac tgtcggcagc accgacgagc
     4621 gtgagctcga tgaaatgatc gccgcggacg gtcgcaaggg ggagatctac ggccggcttc
     4681 gcgctctccg ggagaggtac gcggatcaga tccgagctcg atttcccgat atcccccgcc
     4741 gtgtctccgg gtacaacctg gacgagctcc tcccggagag ccgcttccac gtcggccgct
     4801 ccctcgtcgg gactgaagga acctgcgtcg ttgtccttcg cgcgaagcta cggctcgtgc
     4861 ctagcccgcg gcaccgcgcg ctcctcgtcc tcggcttccc cgacgtccac gtagcagcgg
     4921 accacgtccc cgatgtgctg gcg
//
LOCUS       DATMBB010000282         3906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_123908, whole genome shotgun sequence.
ACCESSION   DATMBB010000282 DATMBB010000000
VERSION     DATMBB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123908"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VNL92_06080"
     CDS             complement(<1..406)
                     /locus_tag="VNL92_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94315.1"
                     /translation="MTNTISEAAAIGVHQVTMPIPFPLKEVHAYLIDAGGEWVMMDAG
                     FPSQEALSILKPEVERICGGPGNVRAVFISHYHPDHCGLAGWLEAHGAEVIVHERDWP
                     SVERMNSDEPVRPEEYGGSAFAAIASATNFSFE"
     gene            complement(415..870)
                     /locus_tag="VNL92_06085"
     CDS             complement(415..870)
                     /locus_tag="VNL92_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWO94316.1"
                     /translation="MRQDLLIRAITAYGRALAVADPIRLRFWDSRGLTMTQLRLMYII
                     YQRNELTISELAELMVVRKPTITGLTDRLVRQKLVRRLSDKTDRRIVRIALTNEGRRV
                     LGEIEVASKAYLDRVFGTMGETQVEEFVRLMESFAASAEQVQQAAEFRP"
     gene            962..2143
                     /locus_tag="VNL92_06090"
     CDS             962..2143
                     /locus_tag="VNL92_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWO94317.1"
                     /translation="MVQQKAPNAMSLFAPEMAENPYPFYAQLRKESPVSKIGPLGLWA
                     IAKYDDCVRILRDPETFSSIVRVPDPEENLPPSMLFNDPPVHTRLRGMISKAFTPRVV
                     ELQRGAIQEYCDRLIDALLVEDEVDIVAGLAYPLPVMVIANMMGVADGDLATFKRWSD
                     EIIENVGQVILSGDQSGLEETNKQFDEYFSARLDKLRREPEDNLLSELVHVETEEGRL
                     TQEDLLMFCRLLLVAGNETTTGLIVNTIRALAEFPEVLRQVRANPDLLPTTIEESLRY
                     YAPFQVTIRRAARDLQVRGETIAAGDRMFVMLASANRDEDQFERPDEFVADRDPNRHV
                     AFGMGIHYCVGAPLARLEADVAMRTLLPRITGIEVEAAEPGSLLRPGGPKSLRVKFSK
                     V"
     gene            2204..2413
                     /locus_tag="VNL92_06095"
     CDS             2204..2413
                     /locus_tag="VNL92_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94318.1"
                     /translation="MSEQFIAMAQDAGIEAVAIRMEDDACEPCRTLILAYAPRDLPLL
                     PFAKCTRAGGCECSYVPLPKRDREA"
     gene            complement(2419..>3906)
                     /locus_tag="VNL92_06100"
     CDS             complement(2419..>3906)
                     /locus_tag="VNL92_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWO94319.1"
                     /translation="TPQFAAMIGIGVGIDYALLITNRFRESRHRGTDVEDAIAEAAGT
                     SGRAVLFAGGTVVIALLGLALIGIPFVAYLGGAASTVVLLSVVVALLVLPALLGLAGP
                     HIDRLHVPGLPRASYESETGIGYHWSRVVQRAPWLWASLALGILLLLAAPLLDIRLGV
                     SDAGSNPESATSRRAYDLLAEGFGVGFNGPIIVGVAVNDPSAVNAVNELPARIEGLEN
                     VASASAPRFNDAGTAATIRVIPETAPQAAETADLVHLLRKEADAWQASQDIEILVGGP
                     TAVFIDLGDKIASRIPFFFVAVIGLSFILLMMVFRSVLVPLKAAVMNLLSIGAAYGVI
                     VAVFQWGWFGDVFGVSRTGPIESFVPIVMFAVLFGLSMDYEVFLVSRIREEYLEHGDN
                     TQAVARGLSSTTRVISAAAAIMIAVFLSFALSEQRIVKEFGLGLATAIFIDATIVRLA
                     LVPSIMQILGRWNWWLPGWLDRLVPHVAIEQTREPERAAAAAGGD"
BASE COUNT          726 a         1289 c         1250 g          641 t
ORIGIN      
        1 gctcgaacga aaagttcgtg gcggaagcga tcgccgcgaa tgcactgccc ccgtactcct
       61 ccggacgaac gggctcgtca gaattcatgc gctccacgct cggccagtcg cgctcatgca
      121 cgatgacctc ggcgccgtgc gcctcgagcc agcccgcgag accacaatgg tcgggatggt
      181 aatgactgat gaacaccgcc cgaacgttgc ccgggccgcc gcagatccgc tctacctccg
      241 gcttcaggat cgagagtgcc tcctgcgagg gaaagcccgc gtccatcatc acccactcgc
      301 ccccggcgtc aatgaggtac gcatgcacct ccttcagggg aaatgggatc ggcatcgtta
      361 cctggtggac gccgatagcg gcggcctcgg agatggtgtt cgtcattgtt tcccctatgg
      421 acggaactcg gcggcctgct ggacctgctc ggcgctcgcg gcgaagctct ccatcagccg
      481 cacaaactcc tcgacctgcg tttcccccat cgtcccgaac accctgtcca ggtacgcctt
      541 gctcgcgacc tcgatctccc cgagcacgcg gcgcccctcg ttggtgaggg cgatgcgtac
      601 gatgcgacgg tcggtcttgt cgctcaggcg ccgaacgagc ttctgcctga cgaggcggtc
      661 ggtgagcccg gtaatggtcg gctttcggac gaccatcaac tcggcaagtt cgctgatcgt
      721 caactcgttg cgctggtaga tgatgtacat cagccgtagc tgggtcatcg tcagtccgcg
      781 actgtcccag aaccgcagcc ggattgggtc agcgacggcc agcgcccgcc cgtacgcggt
      841 gatcgcccga atgagcaggt cttgcctcat gccggcccct tcccgtagcc tgagatagtt
      901 tcggtaagac taacagttag tttagcctcc gggtatgtgc ccggcaaacc gaggtggtgc
      961 catggtccag caaaaggccc ctaacgcgat gagcctcttc gcgcccgaga tggcggaaaa
     1021 cccctatccg ttctacgccc agctccggaa ggagtcgccg gtctcgaaga tcgggccgct
     1081 cggcctctgg gcgatcgcca agtacgacga ctgtgtgcgc atcctgcgcg accccgagac
     1141 cttctcatcg atcgtgcgcg taccggatcc ggaagaaaat cttcccccaa gcatgctgtt
     1201 caacgatccg ccggtgcaca cccgcctgcg cggcatgatc agcaaggcgt tcacgccgcg
     1261 cgtcgtcgag ctgcagcgcg gagcgatcca ggagtactgc gatcggctga tcgatgcgct
     1321 gcttgtcgag gacgaggtcg acatcgtagc ggggctcgcc tacccgctac cggtgatggt
     1381 catcgccaac atgatgggcg tcgccgacgg cgacctggca acattcaagc gctggtcgga
     1441 cgagatcatc gagaacgtcg gccaggtgat cctgtcgggc gaccagagcg gcctcgaaga
     1501 aaccaacaag cagttcgacg aatacttctc agcgcgcctc gacaaactgc gccgggagcc
     1561 tgaggacaac ctgctcagtg agctcgtgca tgtcgagacc gaggagggcc gcctcacgca
     1621 ggaagacctg ctgatgttct gccggctatt gctcgtcgcc ggcaacgaga cgaccactgg
     1681 tctcatcgtc aacacgatcc gggcactggc ggaattcccg gaggtcctgc ggcaggtacg
     1741 ggcgaatccc gatctgctgc cgacgacgat cgaagagtcg ctgcggtact acgcgccgtt
     1801 ccaggtcacg attcgccgcg ctgcgcgcga tctccaggtg cgcggcgaga ccatcgcagc
     1861 cggcgaccgc atgttcgtga tgcttgcgtc cgcgaaccgg gacgaggatc agttcgagcg
     1921 cccggacgaa ttcgtcgccg atcgggaccc gaaccgtcac gtcgccttcg gcatgggcat
     1981 ccactactgc gtcggtgcgc cgctggcccg cctggaggcc gatgtcgcga tgcggacgct
     2041 gctcccgcgc atcaccggca tcgaggtgga ggccgccgag ccgggctcgc tcctgcgccc
     2101 cggtggtccg aagtcgttgc gggtgaagtt ctccaaggtt tgactagccg tggacggcgc
     2161 agccgaacct ggggcgaatg catcgggcgg tagcccgacc tggatgagcg agcagttcat
     2221 cgcgatggcc caggacgccg gcatcgaggc cgtcgccatc cgaatggagg acgacgcctg
     2281 cgagccttgt cgaaccctca tcctggctta cgcgccgcgg gatcttccgt tgctgccgtt
     2341 cgcgaagtgc acgcgcgccg gtggttgcga gtgttcgtac gtgccgctcc cgaagcgcga
     2401 ccgcgaggct tagccgcctt aatcgccgcc ggcagccgcg gcagccctct ccggctcgcg
     2461 ggtctgttct atcgcgacgt gcggaacgag tcggtcgagc cagccgggga gccaccagtt
     2521 ccagcgcccg agaatctgca tgatggatgg gacgagggcg aggcgcacga tcgtcgcgtc
     2581 gatgaagatc gcggtggcaa ggcccagacc aaattccttc acgatgcgct gctcgctcag
     2641 ggcaaagctc aggaacaccg cgatcatgat cgccgcggcg gcggagatca cgcgtgtcgt
     2701 cgacgagagc cctcgcgcca cggcctgcgt gttgtcaccg tgttcgaggt actcctcacg
     2761 gatgcggctg acgaggaaga cctcgtaatc catcgagagt ccaaacagca cggcgaacat
     2821 cacgatgggc acaaacgatt cgatcggccc tgtcctgctc acgccgaaca catcgccaaa
     2881 ccatccccac tggaagacgg cgacgattac accgtaggcg gcgccgatcg acaggagatt
     2941 catcacggcg gccttcaatg ggaccagcac ggacctgaac accatcatga gcaggatgaa
     3001 gctgaggccg atgacggcga caaagaagaa cggtattcgc gacgcgatct tgtcgccgag
     3061 atcaatgaag acggcggtcg ggccaccgac cagaatctcg atgtcctggc tcgcctgcca
     3121 tgcgtccgcc tctttccgca gaaggtgcac caggtccgcc gtctcagccg cctgcggcgc
     3181 cgtctctgga atgacccgga tcgtggcggc ggtgcccgcg tcattgaagc gtggtgctga
     3241 ggcgctggcc acgttctcca gtccctcgat ccgcgctgga agctcgttga cggcattgac
     3301 ggctgaggga tcgttgacag cgacgccgac gatgatcggg ccgttgaagc cgacgccgaa
     3361 accttccgcc aacaagtcgt aggcgcggcg ggatgtcgcc gactcgggat tgctgccggc
     3421 gtcggacacg ccgaggcgaa tgtccagcag cggggcggca agaagcagga gaatgccgag
     3481 cgccagcgac gcccagagcc agggagcgcg ctggacaacg cggctccagt gatagccaat
     3541 tccggtctcg ctctcgtacg acgccctcgg cagcccggga acgtgcaggc ggtcgatgtg
     3601 aggcccggcc agtccgagga gcgccggcag caccaggaga gcgacaacga ccgagaggag
     3661 cacaaccgtg gacgccgccc cgccaaggta ggcgacgaag gggatgccaa tgagcgctag
     3721 gccgaggagc gcgatgacga ccgtcccgcc ggcgaacagc accgcccgcc cggaggtccc
     3781 cgcggcttcc gcgatcgcgt cctcaacatc tgtgccgcgg tgccgggact cccgaaagcg
     3841 attcgtgatc agcagcgcat agtcaatgcc cacgccgatc ccgatcatcg ccgcgaactg
     3901 cggggt
//
LOCUS       DATMBB010000283         3701 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_450671, whole genome shotgun sequence.
ACCESSION   DATMBB010000283 DATMBB010000000
VERSION     DATMBB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450671"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VNL92_06105"
     CDS             <1..920
                     /locus_tag="VNL92_06105"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWO94320.1"
                     /translation="IGGRTIHTYHTEGAGGGHAPDIIRLAGEPNVLPSSTNPTRPYTV
                     NTLAEHMDMLMVCHHLNPRVPEDVAFADSRIRPETMAAEDVLHDIGALSMYSSDSQAM
                     GRAGESFTRLLQTAHKMKEVRGALPEERAGDDNARILRYLAKLTINPARAHGIAAFVG
                     SIEPGKLADIVLWPIEFFGVKPALVIKGGTIAWALMGEPNASIPTVEPMLFRPMFGAH
                     RTVVASTCVTFTSQAALAGGLRGRLGLARRVEAVAGCRMLGKADMLRNDALPRIDVDA
                     ESYRVFVDGELATAPPAEQLPLAQRYFLI"
     gene            917..1615
                     /locus_tag="VNL92_06110"
     CDS             917..1615
                     /locus_tag="VNL92_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HWO94321.1"
                     /translation="MIGDAGPAPLLALLQIGDSAFPIGAYAHSYGLEELIREGRIDGP
                     DAAIAYIASTVVGPAITADAVATARVCEAMRLGDLATAVEVDHDLHAMKGASALRQAS
                     TSQGRRLLEELAATCPTDAVATFLGAIRDGATPGTHAAALGVAAAGYDAPPASAAAVL
                     ALGLATAMLQAAMRLLPLSHRDVQKALHQLRPTIAEASARLAAEPPPLAALHPLQEIA
                     AMRRRCAAVRQFAS"
     gene            1629..2267
                     /gene="ureG"
                     /locus_tag="VNL92_06115"
     CDS             1629..2267
                     /gene="ureG"
                     /locus_tag="VNL92_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWO94322.1"
                     /translation="MGKALCVGVGGPVGSGKTALVQQLVPRLLAAGRRPSIVANDIYT
                     TEDASALRSSLAGVIEAGRIVGVETGGCPHAAVRDDPTANLEAVAQLMNDWPDTDIVL
                     IESGGDNLTLTFSPLLADCSIYVLDVAGGDKMPKKQGPGLVHADLLVINKADLAPYVG
                     ADLARMETDARAVRTGPLLFTDCRTGAGIEPVAAWVNEQALLAPNDAIARSS"
     gene            2264..3097
                     /locus_tag="VNL92_06120"
     CDS             2264..3097
                     /locus_tag="VNL92_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWO94323.1"
                     /translation="MTLSVARPVDGSLDAVFRAGKMGCRVDARAVAPLELRGPFRATN
                     LPPQYFVRNVTGGICGGDRLRTRIEVGEGAAIRIATTSASKVCAMPSAPATSALELVA
                     HRGSVAVYESHATILQRDSDFSQRVRLDVRPGAGVLFSDIVMLGRLAHGERLVFRRFA
                     SELLVQSGAAATPSFIERYEVRPERDPTLDAALGGYGVIGSLYALGTSADGVDLDALA
                     AWCAEDCRAYAGADALPNGAGIVVRFLAPDGWTARSFVRSASRCALSGRDARRNADGQ
                     P"
     gene            3297..>3701
                     /locus_tag="VNL92_06125"
     CDS             3297..>3701
                     /locus_tag="VNL92_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWO94324.1"
                     /translation="MLEVSGLRMVFPNGHEALADISFTVPEGQFVCIIGRSGAGKSTL
                     LRCLNGVLSPTSGRVHVAGVDVTAASEREKIRLRRHIGFVFQEYNLVGRLPVLTNVLV
                     GRLGYTSNWRSMLGLFSRRDRAVALEALARVRL"
BASE COUNT          555 a         1257 c         1276 g          613 t
ORIGIN      
        1 ccatcggcgg tcgcaccatc catacctacc acaccgaggg cgcgggcggc ggacacgcgc
       61 cggatatcat ccggctcgcc ggcgaaccga acgtgctacc gtcgtccacc aacccaacgc
      121 ggccgtacac ggtgaacacg ctcgcggagc acatggatat gctgatggtc tgccatcacc
      181 tgaatccgcg cgtgcccgag gatgtcgctt tcgccgacag ccgcatcagg ccggagacga
      241 tggcggcgga ggacgtgctg cacgatatcg gcgctctgag catgtactcg tccgattctc
      301 aggcgatggg acgtgccggc gagagtttca cccgcctgct acagacggcg cacaagatga
      361 aggaagtgcg tggcgccctg ccggaggagc gcgccggcga cgacaatgcg cgcatcctcc
      421 gctacctcgc gaagctcacg atcaacccag cgcgggctca cggcatcgcc gcgttcgtcg
      481 gatcgatcga gcccggaaag ctcgccgaca ttgtgctgtg gcccatcgag ttcttcggcg
      541 tcaagccagc gctcgtcatc aagggcggca cgatcgcctg ggcgctcatg ggcgagccga
      601 atgcctcgat cccgacggtg gagccgatgc tgttccgccc gatgttcggc gcgcatcgca
      661 cggtcgtcgc gagcacgtgc gttacgttca cgagccaggc ggcgcttgcc ggcggccttc
      721 gcgggcgcct cgggctcgcc cggagggtgg aggccgtcgc cggctgccgg atgctcggca
      781 aggcggacat gttgcggaac gacgctctgc cgcgcattga cgtcgatgcg gagagctacc
      841 gtgtcttcgt cgacggcgag ctggcgacag cgcccccggc cgaacagctg ccgctcgcgc
      901 agaggtactt cctcatatga tcggcgacgc gggaccggcg ccccttctcg cgctcctcca
      961 gatcggcgat tccgccttcc ccatcggcgc gtacgcccac tcgtacggcc tggaggagct
     1021 gattcgtgag ggccgtatcg atgggccgga tgccgccatc gcctacatcg ccagcaccgt
     1081 ggtggggccg gcgatcacgg cagacgcggt ggccacggca cgggtttgtg aggcgatgcg
     1141 tctgggcgat ctcgcgacgg ccgtagaggt cgaccacgac ctccatgcga tgaaaggggc
     1201 gtcggctctg cggcaggcat cgacgtcgca ggggcggcgg ctccttgagg agttggctgc
     1261 aacctgccca acggacgcgg tcgcgacctt cctgggggcg attcgggacg gcgcgacgcc
     1321 cggtacgcat gcggcggccc tcggcgttgc ggctgccggg tacgatgcgc cacctgcctc
     1381 tgcggcggct gtgctcgcgc ttggcctcgc cacggccatg ctccaggcgg cgatgcgatt
     1441 gctgccgcta tcgcaccgag acgtacagaa ggcgctccac cagcttcgcc ccacgatcgc
     1501 cgaggcctcg gcacgcttgg cggcggagcc ccctccgctg gcagcgctcc atccgcttca
     1561 ggagattgcc gcgatgcgga ggcggtgcgc agccgtccgg cagttcgcga gctgaaaggg
     1621 gggagtggat gggcaaggcc ttgtgtgtcg gtgtcggggg gcccgtgggc tcgggcaaaa
     1681 cggcgctggt ccagcagctc gtgccgcgac tgttggccgc ggggcggagg ccgagcatcg
     1741 tcgcgaacga catctacacg acggaggatg cgtccgcgct gcgcagctcc ctcgccggcg
     1801 tcatcgaggc gggccgcatc gtcggcgtcg aaacgggcgg atgcccgcat gccgccgtgc
     1861 gcgacgatcc gaccgcgaat ctcgaggccg tggcgcagtt gatgaacgac tggccggaca
     1921 ccgatatcgt gctcatcgaa agtgggggcg ataacctgac gctgacgttc agcccgctcc
     1981 tcgccgattg ctccatctac gtgctcgacg tcgcgggtgg cgacaagatg ccgaagaagc
     2041 aaggtccggg cttggttcac gccgatctgc tcgtcatcaa caaggccgat ctcgcgccgt
     2101 atgttggcgc ggacctcgcg cggatggaga ccgatgcccg ggccgtgcga accggccccc
     2161 tgctgttcac cgattgtcgc accggcgcgg gcatcgagcc ggtcgcggcc tgggtcaacg
     2221 agcaggcgct cctcgccccc aacgatgcga tcgcgcgatc atcatgacgc tcagcgtcgc
     2281 tcgtcccgtc gacggctctc tcgacgccgt gttccgggcc ggcaagatgg gctgccgcgt
     2341 ggatgccagg gcggtagcgc cgctggagct gcgggggccg ttccgcgcta ccaaccttcc
     2401 tccgcagtac ttcgttcgca atgtcaccgg aggcatctgc ggcggcgacc gcctcaggac
     2461 gcggatcgaa gtcggagagg gcgcggccat ccgcatcgcg accacgtcgg ccagcaaggt
     2521 atgcgcgatg ccctcggctc cggccacgag cgcgcttgaa ctcgtcgctc atcgcggaag
     2581 cgtcgcggta tacgaatcgc acgcgacgat cctccagcgc gacagcgact tcagccagcg
     2641 cgtccgcctc gacgtgcgcc ctggtgctgg cgtgctgttc agcgacatcg tgatgctggg
     2701 acgcctcgcg catggcgagc ggctcgtgtt tcgccgcttc gcgagcgagc tcttggtgca
     2761 gagcggagcc gcggccactc cctcgttcat cgagcgatac gaggtgcgcc ccgaacgtga
     2821 cccaaccctc gacgccgctc tcggtggcta tggcgtcatc ggctctcttt acgcactggg
     2881 cacgagcgct gacggcgtcg atctcgacgc gctggcggcg tggtgcgccg aagactgccg
     2941 tgcctacgcg ggcgccgatg cgctaccgaa cggcgccggc atcgtcgtgc gcttcctcgc
     3001 cccggacgga tggaccgccc ggagcttcgt acggtcggcg tcccggtgcg ccctttcggg
     3061 ccgtgacgcg aggcgtaacg cggacggcca accgtagcga tcgctgcccc aagcgcatgg
     3121 ccccctgacg agcggagggc tgagtccact tggccggcga catgtttcga cggtgcctta
     3181 ccagatgctt gccttccgag ccgcggcctt aaccctggtc gctgcggcct tgagtagtct
     3241 cctccgcgaa agaagccgac gagttccgtc gccggcagtc cgagaggagg cgccgcatgc
     3301 tcgaagtgag cgggctccgg atggtcttcc ccaacgggca cgaggccctc gccgatatct
     3361 cgtttacggt cccggagggg cagttcgtct gcatcattgg gcggagcggg gcgggcaagt
     3421 ccacgctgct tcgctgcctc aacggggtgc tctcgcccac atcgggccgc gtgcacgttg
     3481 ccggcgtcga cgtcaccgcg gcttcggagc gagagaagat caggctgcgg cgccacatcg
     3541 gcttcgtctt tcaggagtac aacctcgtcg gccggttgcc ggtgttgacc aatgtgctgg
     3601 taggccggct cggctacacc tcgaattggc ggagcatgct cgggctcttc tcacggaggg
     3661 accgagcggt cgccctcgaa gccttggccc gtgttcgatt g
//
LOCUS       DATMBB010000284         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_218603, whole genome shotgun sequence.
ACCESSION   DATMBB010000284 DATMBB010000000
VERSION     DATMBB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218603"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..968
                     /locus_tag="VNL92_06130"
     CDS             159..968
                     /locus_tag="VNL92_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO94325.1"
                     /translation="MTSAGQRRIAVVVGGSRGIGRAIAERLAREGCHVIVNFSRREDD
                     AIATVESIERAGGSAETHQANIGEIEAIRAMFGDIGARLGRVDVLVGNAARGIERPRN
                     ALRAMPKHLRATFDVNVLGPWFCAQEAAKIMPRGGRIVNISSLGAQRYAPRYAAVGVT
                     KAALEALTRYLAVELAPQGIAVNAVSAGLVESSEGLRMFPDHVSDVLRRRVPAGRNVQ
                     PEDIAGLVAFLCSEDAAMIVGQTIMMDGGFSLLGWDLTGFFEGPSDDPAAL"
     gene            complement(980..2206)
                     /locus_tag="VNL92_06135"
     CDS             complement(980..2206)
                     /locus_tag="VNL92_06135"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWO94326.1"
                     /translation="MNRVVVTGLGAICPVGNTAEDAWKALLAGKSGIDYISTFDVSSF
                     PVKVAGEVRGFDPQTHMDRKVARRSGRFSQFALVAATEALQSARLEVTEANRDGIGAI
                     VATSGGGFDTGPAYVTLQERGPNRVDPVLIMRVGGHMAGVRVGRELGIRGPNSTVNSA
                     CASGNDAIGQALNMLRLGHAQAILAGGAEAALNGVGLAALANVGALSRSERPPQEVCR
                     PFDAERDGFVMAEGAGILLLETEEHARERGAPIICELAGAGWSFDATDETAPDAEGQA
                     LAMRRAMADASVGPEDVSYVNAHGTSTQLNDAAETRALKLALGEAAAATPISSIKSMA
                     GHLAAGAGGLESVMSVFAIRDGMVPPTINYEHPDPACDLDYVPNVARKVDVEAVLTNS
                     FGLGGQNCCLVFRRYR"
     gene            2327..2551
                     /locus_tag="VNL92_06140"
     CDS             2327..2551
                     /locus_tag="VNL92_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94327.1"
                     /translation="MPSERIQRQVDRLLDEAEAAVSEGNWQLAGELTRRVLTLDPESV
                     ASRNHPSIDRRSWPEASHPAPAAARPIEPD"
BASE COUNT          393 a          895 c          879 g          420 t
ORIGIN      
        1 ctcggagtcg atggggtctt cgtcgtcggt cacggcgcaa ccacggaaga gggcgtcacc
       61 tcgatggtcc gggaggccgc ctcgctcgtg cggcgcgact tcgtgacgtg gttgcgcgag
      121 gcgctgcagc gcacggcgca agctgtggag cgtgtgtcgt gacttccgcc ggacaacggc
      181 gcattgccgt cgtggtcggc ggctcgcgcg gcatcggccg ggctatcgcg gagcggctgg
      241 cccgcgaagg atgccatgtc atcgtcaact tcagccgccg agaggacgat gcgatcgcca
      301 ctgtcgaatc gatagagcgg gccggcggct ccgccgagac gcatcaggcg aacatcggcg
      361 agatcgaggc catccgagcg atgtttggtg atatcggggc gaggctcggt cgcgtcgacg
      421 tgctcgtggg caacgcggcg cgcggaatcg agcggccacg aaacgccctt cgcgccatgc
      481 cgaagcactt gcgcgccacg ttcgatgtca acgtgctggg gccgtggttc tgcgcgcagg
      541 aggccgcgaa gatcatgccg cggggcggcc gcatcgtgaa catctccagt ctgggggcac
      601 agcgctatgc gccgcggtac gcggcggtcg gggtgacgaa ggccgcgctc gaggcgctca
      661 cacgctacct ggccgtggag cttgcgcctc agggcatcgc cgtgaacgcg gtgtcggccg
      721 gcctcgtgga aagcagcgag gggcttcgca tgttccccga tcacgtttcc gatgtgctcc
      781 gaaggcgggt ccctgcgggc cgcaacgtac aaccggagga catcgccggc ctcgtcgcgt
      841 tcctctgcag cgaggacgct gccatgatcg tcggccaaac gatcatgatg gacggcggat
      901 tctcgttgct cgggtgggac ctcacgggct tcttcgaagg accgtccgac gatccggcag
      961 ccctgtgacc tgatgcccgc tagcgatacc tccggaagac gaggcagcaa ttctgtccgc
     1021 caaggccgaa ggagttcgtg agcaccgcct ccacgtccac cttccgcgcc acgttcggaa
     1081 cgtagtcgag gtcgcaggcc gggtcggggt gctcgtagtt gatggtcggc gggaccatcc
     1141 cgtcgcggat ggcgaacacg ctcatcacgg actccagccc gcccgcaccc gccgccaggt
     1201 gccctgccat cgacttgatg gagctgatcg gcgtcgccgc ggcggcttcc cctagggcga
     1261 gcttgagcgc ccgcgtctca gcggcgtcgt tgagctgggt cgaggtaccg tgcgcgttca
     1321 cgtagctcac gtcttctggc ccgacactcg catcggccat ggcgcggcgc atggccagcg
     1381 cctggccctc cgcgtcgggc gccgtttcgt cggtggcgtc gaacgaccac ccggcgccgg
     1441 ccagctcgca gatgatgggc gctccccgct ccctcgcgtg ctcctccgtc tcgagcagca
     1501 ggatgccggc gccttccgcc atgacgaagc catcccgctc ggcgtcgaac ggacggcaca
     1561 cttcctgggg aggccgttcg ctgcgcgaca gggctccgac gttggccagc gccgccaatc
     1621 ccacaccgtt cagcgccgcc tcggccccac cggccaggat cgcctgcgca tgaccgaggc
     1681 gcagcatgtt gagcgcctgg ccgatggcgt cgttgccgct ggcacaggcg ctgttcaccg
     1741 tgctgttcgg tccgcggatg ccgagctcgc ggccgacgcg aacacccgcc atgtgcccgc
     1801 cgactcgcat gatcagcacc gggtcgactc gattcgggcc gcgctcctgg agggtgacgt
     1861 acgccgggcc cgtatcgaac ccgcccccgc tcgtcgcaac gatcgcgccg atgccatcgc
     1921 gattcgcctc cgtcacctcg aggcgggcgg actgcagcgc ttccgtcgcg gcgacgagcg
     1981 cgaactggct gaagcgtccg ctgcgccggg cgaccttgcg gtccatgtgg gtttgcggat
     2041 cgaatccgcg cacctcgccg gcgaccttca ccggaaacga ggacacgtcg aaggtgctga
     2101 tgtagtcgat gcccgacttg cccgcgagca acgccttcca ggcatcctcc gccgtgttcc
     2161 ccacggggca aattgccccg aggccggtta cgacgacgcg attcatcgat ggcctcctca
     2221 tcgtgcgcta cactacgccg cctcagattg tactctccgg ccgtacggat gcccagccgc
     2281 cgggcgaccg aggcgcgtcg cacggacgtg ctatcatgcc gctgctatgc ccagcgaacg
     2341 tattcaacgg caggtcgatc gactgcttga cgaggcggag gcggccgttt cggaaggcaa
     2401 ttggcagctc gccggcgagc tgacgcgacg cgtcctcacg ctcgatcccg aatccgtggc
     2461 cagccgcaac cacccttcca tagaccggag gagttggccg gaggcatcgc acccggcacc
     2521 cgcggcagcg cgcccgatag agccggactg atcagcgagt agccgcgatg cctctagagc
     2581 gggttgg
//
LOCUS       DATMBB010000285         3301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_169419, whole genome shotgun sequence.
ACCESSION   DATMBB010000285 DATMBB010000000
VERSION     DATMBB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169419"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VNL92_06145"
     CDS             <1..413
                     /locus_tag="VNL92_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HWO94328.1"
                     /translation="AALGAAELVYADYRVALRRANPGDFAYLDPPYHPLSSTANFTGY
                     TPLGFGEGEQRELAREVDRLTKVGVAVLLSNSDTPLIRELYAAYEMTEIEVPRSINSR
                     PDRRKGVGELLISNYARLQKGPSANGIMQEAAAM"
     gene            469..2121
                     /locus_tag="VNL92_06150"
     CDS             469..2121
                     /locus_tag="VNL92_06150"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWO94329.1"
                     /translation="MQDLPLRQKLIIMVGVMLGLFFSALLQTSVTTATPHIVASLGGL
                     DLFSWVFTIYILTSTVSIPIFGKLSDIYGRKLLYLASLVVFIAGSAMAGAAQDMPQLI
                     AFRAIQGLGAGGISAIAFTIVGDIFAPQERGRWEGIVAGTFGVSSVVGPLVGGYIADN
                     WSWRWVFYINLPVGVFAFGVIWAVLPQVKGIARGSIDYLGTASLVGSVSPLLLALLWG
                     GTEYPWSSPVIIGLFVASAAILIVFVMTQARVEEPILPLELFANGLFSVSMVATFLTG
                     AAMFALTAYLPLFVQAVIGLSATNSGLVVMPLMIGLVTGAIISGAVMTRTGRYKFLLV
                     GGTAILTVGLYLTSRMDADASQAEAVRNMVICGIGIGPTAPLFLIIVQNALPHRYLGV
                     ATSSVQFFRQMGGTIGVAVMGTVLNNRIASELDGALPPAVREANAPPLRDALEQPELL
                     LNDGAATQVGQMFAQFGDDASTLFDQSIEALRVSLANSLETVFLIGLGAAALSFITTV
                     ILREIPLRTTEEIMEELRAEGAAPEVAGPAVPAEGGAEPTPS"
     gene            complement(2141..>3301)
                     /locus_tag="VNL92_06155"
     CDS             complement(2141..>3301)
                     /locus_tag="VNL92_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWO94330.1"
                     /translation="AVEGLREGGHEGRITLVGAETHLPYDRPPLSKQLLAGAWESDRL
                     TYRDGDAMSALNVTTMLGRRAVSLDLQNRTVGLDTGEPLSFDGLVIATGASPRTLPGT
                     PALPGIYTLRTLDDALALRAEFERGPRVAVVGAGFIGAEVAATARERGLDVTLIEAFP
                     VPLVRAIGEEMGRICATLHFDHGVDVRLGVGVEAFGGTGRLERVLLSDGTAVEADVAI
                     VGIGVMPNTQWLESSGLELRDGVVCDAYCQAGAPGVYAAGDVARWYNPLFEEEMRVEH
                     WTNAAEQGRAAALNLLAGAEKSTEYSPVPYFWSDQYGLTLQFVGHHGPGDEVEVVHGS
                     PDERKFVALYRRGDRLVGAFGMAWSRIVMRYSSLIAKRATWDEALAFAKTLG"
BASE COUNT          534 a         1144 c         1037 g          586 t
ORIGIN      
        1 gcgctgccct gggcgccgcg gagctggtgt acgccgacta tcgggtcgcc ctgaggcgcg
       61 cgaatcctgg cgacttcgcc tacctcgacc cgccgtacca tccgctctcc agcaccgcca
      121 actttacggg ctacacgccc ctcggcttcg gcgaagggga gcaacgggag ctggcacgag
      181 aagtggatcg cctcaccaaa gtaggcgtcg ccgttctgct cagcaactcc gacacgcccc
      241 tcatccggga gctgtacgcc gcgtacgaga tgacggagat cgaggtcccg cggtccatca
      301 actcgcgacc ggaccggcgc aagggagtgg gcgagcttct catctcgaat tacgcgcgat
      361 tgcagaaggg cccgagcgcc aacggcatca tgcaagaagc tgcagcaatg tagtattatc
      421 gcctccccgc acgtgagccg cccgtgagcg atgagacgag catcgtcgat gcaagacctc
      481 cctcttcgcc agaagctcat catcatggtc ggcgtcatgc tcggcctgtt cttctccgcg
      541 ctcctgcaaa catccgtgac cacggccacg ccgcacatcg tcgccagcct gggtggactc
      601 gacctgttca gctgggtctt cacgatctac atcctcacgt ccaccgtctc catcccaatc
      661 ttcggcaagc tatccgacat ctatggacgg aaactgctgt atctcgcttc gctcgtcgtg
      721 ttcatcgcgg gctccgccat ggccggagcg gcgcaggaca tgccgcagct catcgccttc
      781 cgcgccatcc agggtctcgg ggcgggcggc atcagcgcca tcgccttcac gatcgtcggc
      841 gacatcttcg caccgcagga acgcgggcgc tgggagggca tcgttgccgg cacattcggg
      901 gtatcgagcg tcgtgggtcc gctcgtgggc ggctacatcg ccgacaactg gtcgtggcga
      961 tgggtcttct atatcaactt gccggtggga gtgtttgcct tcggtgtcat ctgggcagtt
     1021 ctgccacagg tgaagggaat cgcgcgcggc agtatcgatt acctcggcac agcgtcgctc
     1081 gtgggttcgg tgagcccgct actgctcgcg ctgctctggg gtggcaccga gtatccgtgg
     1141 agctcgcccg tcatcatcgg gctgttcgtc gcctcagccg ccatcctgat cgtgttcgtc
     1201 atgacccagg ctcgcgtgga ggagcccatc cttcccctgg agctgttcgc caacgggctc
     1261 ttctccgtat ccatggtcgc gacattcctg acgggcgccg cgatgttcgc cctcacggcc
     1321 tacctgccgc tcttcgtgca ggcagtcatc ggcctctcgg ccacgaacag cggcctcgtc
     1381 gtgatgccgc tgatgatcgg cctggtgacg ggcgccatca tcagcggcgc cgtcatgacg
     1441 cgcacgggcc ggtacaagtt cctgctcgtg ggaggcacag ccatcctcac ggtcggcctc
     1501 tatctgacaa gccgcatgga tgccgacgcg agccaagcgg aagcggttcg caacatggtg
     1561 atctgcggca tcggcatcgg ccccacggca cccctgttcc ttatcatcgt gcagaatgct
     1621 ctgccgcacc gctatctcgg cgtcgccacg tcgagtgtgc agttcttccg gcagatgggc
     1681 ggaaccatcg gcgtggcggt tatgggaacg gtgctcaaca atcgcatcgc cagcgagctc
     1741 gacggggcgt tgcccccggc cgttcgggag gcgaatgccc cacccttgcg cgatgccctg
     1801 gagcagcccg agctgctcct gaacgacggg gcggcgaccc aggtgggcca gatgttcgcg
     1861 cagttcggcg atgacgcctc gacgctgttc gatcagtcga tcgaggcgct gcgcgtgtcg
     1921 ctcgcgaact cactcgagac ggtgttcttg atcgggctcg gcgcagcagc cctgtccttc
     1981 atcacgaccg tcatcctgcg ggaaatcccg cttcgcacca ccgaggagat catggaagag
     2041 ctgcgggccg agggcgcagc tcccgaggtt gccggccccg ccgttccggc ggagggcggc
     2101 gcggaaccga cgcccagcta gccaccgcgc cttggtctgc tcatccgagc gtcttggcga
     2161 aggcgagcgc ctcgtcccac gttgcccgtt tggcgatgag gctgctgtat cgcattacga
     2221 ttctcgacca ggccatgccg aacgctccca ccagcctatc gccgcggcga tacaacgcca
     2281 cgaacttgcg ctcgtcgggc gagccgtgca caacctcgac ctcgtcccca ggcccgtgat
     2341 ggcccacgaa ctggagggtg aggccatact gatcggacca gaagtacggg accgggctgt
     2401 attcggtgct cttctcggcg ccggcgagca gattgagggc cgcggcgcgt ccttgctcgg
     2461 cggcgttcgt ccagtgctca acccgcatct cctcctcgaa gagcgggttg taccagcgag
     2521 ccacatcgcc cgcagcgtac acgcccggcg cgcccgcctg acagtaggcg tcgcacacca
     2581 caccgtcgcg cagctccaga ccggacgact ccagccattg tgtgttgggc atgacgccta
     2641 tgccgacgat ggcgacatcg gcttctacgg cagtgccgtc gctgagcagc acgcgttcga
     2701 gacgcccagt cccgccgaat gcctccacgc ccacgcccag ccgcacatcc acgccgtggt
     2761 cgaagtgaag cgtcgcgcaa attcggccca tctcctcgcc gatcgctcgc acgagtggga
     2821 cggggaacgc ctcgatgagc gtgacgtcca ggcctcgctc tcgcgccgtc gccgctacct
     2881 cggccccgat gaacccggcg cccaccacgg cgacgcgggg tccgcgctcg aactcggcgc
     2941 gaagcgcgag cgcatcatcg agcgtgcgca gcgtgtagat gccgggaagg gcgggtgtgc
     3001 ccggcagggt gcgcggcgat gccccggtcg ctatgacaag gccgtcgaac gacagaggtt
     3061 cgccggtatc gagcccaacg gtgcggttct gcaggtcgag cgagacagct cgcctgccga
     3121 gcatcgtcgt gacgttcagc gccgacatcg cgtcgccatc gcgataggtc aagcggtcgg
     3181 attcccacgc ccccgccaac agctgtttcg agagcggagg gcggtcgtag ggaagatgcg
     3241 tctcggcgcc gaccagcgtg atgcgccctt cgtgtcctcc ctcacgcagc ccctcgacgg
     3301 c
//
LOCUS       DATMBB010000286         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_530653, whole genome shotgun sequence.
ACCESSION   DATMBB010000286 DATMBB010000000
VERSION     DATMBB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530653"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VNL92_06160"
     CDS             complement(<1..684)
                     /locus_tag="VNL92_06160"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HWO94331.1"
                     /translation="MDQHPYISNTEADRAAMLARIGVPDSAALFADIPAAALDPPIDL
                     PPSLSEPELLAEMAALAERNAHGGQMLSFLGGGAERHFIPSVVPYVLSRSEFATAYTP
                     YQPEISQGTLQTAFEFQTMTCELLDLDVANTGMYDGATAFAEACLMACAVTGRRHIAV
                     TDSVHPRYVETVRTYAFGRDLVVDLIDAQHPTVTDQHACLASQQPGFLGNLQDMERLE
                     RLAHSAGALF"
     gene            complement(700..1092)
                     /gene="gcvH"
                     /locus_tag="VNL92_06165"
     CDS             complement(700..1092)
                     /gene="gcvH"
                     /locus_tag="VNL92_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWO94332.1"
                     /translation="MEVPSDLRYTREHEWVRVEDAVGVVGITDHAQDELGDIVFVDLP
                     EEGSELLQFEKLGEIESVKAVSDLFAPVTGEVIERNTRLEDEPELVNRSPYGEGWMLK
                     VTLTNPRELEQLMTADEYERYVREETGE"
     gene            complement(1093..1929)
                     /locus_tag="VNL92_06170"
     CDS             complement(1093..1929)
                     /locus_tag="VNL92_06170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO94333.1"
                     /translation="MARREVLLSYYRRLRMKSPKETLRALAHPGRFAAHVRNIAAGGV
                     REHTNPYRESLSRDEEAAFCADLLSCSVEDVCSAFDEVAADTPFVREVRSRYQEGRPN
                     AESLHIGRFRVWYALVRLARPNVVLETGVHDGLSSALILEALRANGAGHLVSIDLPAL
                     DLPGGEPGWLVPTRLRERWTLELGDARRLLPATLERYPAVDIFIHDSDHRREHREFEF
                     RTVRPHAGPGALLMSDDDEERDVLDDLAREWSAKRCWIRCSLVEGDVAHLGGLRLPAE
                     GG"
BASE COUNT          351 a          668 c          672 g          341 t
ORIGIN      
        1 gaagagcgcc ccggccgaat gggcgaggcg ctcgaggcgt tccatgtcct gcaggttgcc
       61 gaggaagcca ggctgctgcg aggcgagaca cgcatgctga tcggtcacgg tgggatgttg
      121 cgcatcgatg aggtcgacga cgagatcgcg gccgaaggcg taggtgcgaa cagtctcgac
      181 ataacgaggg tggacgctgt cggtcacggc gatgtggcgg cgtccggtaa cggcgcatgc
      241 catcaggcag gcttccgcga aggcggttgc gccgtcgtac atgccggtat tcgccacatc
      301 gaggtcgagc agctcgcatg tcatcgtttg gaactcgaat gcggtctgca gcgtgccttg
      361 gctgatctcc ggttgatagg gcgtatacgc tgtggcgaac tcgctgcggc tgagcacgta
      421 agggacgacg ctcgggatga agtgacgctc ggcgccgccg ccgaggaagc tgagcatctg
      481 ccctccatgc gcgtttcgtt cggcgagcgc cgccatctcc gccaaaagct ccggttccga
      541 gagagagggc ggcaggtcga tcggcgggtc gagcgcggcg gccgggatat cggcgaacaa
      601 tgcggcggaa tcgggcacgc caatgcgagc gagcatggcg gcgcgatcgg cttcggtatt
      661 cgagatgtag gggtgctggt ccataaccac ggcgagacgc tattcgccgg tttcctcccg
      721 cacgtagcgt tcgtactcgt cggctgtcat gagctgctcc agctcgcgcg gattcgtaag
      781 cgtgaccttg agcatccagc cctctccgta gggcgagcgg ttcacgagct ccggctcatc
      841 ctccaatcgc gtattccgtt cgatgacctc gcccgtaacg ggcgcgaaca ggtccgagac
      901 ggccttcacg gactcgatct cgccaagctt ctcgaactgc agcagttcgg aaccttcctc
      961 cgggaggtcg acgaagacga tgtctccgag ctcatcctgg gcgtggtccg tgatgccaac
     1021 gaccccgacg gcgtcctcga cacggaccca ctcgtgctcc ctcgtgtaac ggagatcgga
     1081 aggaacttcc atctaccctc cctcggccgg cagccgcagt cccccaaggt gagcgacgtc
     1141 gccctcgaca agcgaacatc gtatccagca tcgcttcgcc gaccactcgc gagccagatc
     1201 gtcgagcacg tcacgttcct cgtcatcgtc cgacatcaga agtgcgcccg ggcccgcgtg
     1261 cggacggacg gtgcggaact cgaactcccg atgttcgcgg cggtgatcgg agtcgtggat
     1321 gaagatatcg acggcaggat agcgctccag cgtagcggga agcaaccgcc gggcatcgcc
     1381 tagctccaac gtccagcgct cccgcagcct cgtcggtacc agccaccccg gctcccctcc
     1441 cggcagatcg agggcaggca ggtcgatgct gacgaggtgg cccgcgccgt tcgcccgcag
     1501 cgcttccaag atgagcgcgg acgagagacc atcgtgcacg ccggtctcca gcaccacgtt
     1561 cggccgggcc aggcgcacca gcgcgtacca cacgcggaac cgcccgatat gcaggctctc
     1621 cgcgttgggg cgtccctcct ggtaccgcga ccggacctcc cggacgaagg gcgtgtcagc
     1681 cgctacctcg tcgaacgcgg aacagacgtc ttcgacggag cagctcagca ggtcagcgca
     1741 gaacgcagct tcctcgtcgc ggctgaggct ctcgcggtac gggttcgtgt gctcgcgaac
     1801 gcctcccgcg gcgatgtttc gcacgtgcgc cgcgaagcgg cccggatgcg cgagcgcccg
     1861 caacgtctcc ttcgggctct tcatgcggag cctgcgatag tacgagagaa ggacctcgcg
     1921 gcgtgccacg cgcgtcatcc ttacggttgc ctgccgcggc gatagaaggg gcgttccaca
     1981 atccgcgccg gcaggggctt gccccggaca tcgatcgtga cgggcgttcc cg
//
LOCUS       DATMBB010000287         2407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_146863, whole genome shotgun sequence.
ACCESSION   DATMBB010000287 DATMBB010000000
VERSION     DATMBB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146863"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VNL92_06175"
     CDS             <1..1120
                     /locus_tag="VNL92_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWO94334.1"
                     /translation="DDGDADAAGSTATATANGNDVNGASGNTEPDGLESSAELVEQAR
                     PSIVHIETEASALDIFGAPVPQTGVGTGFIIDGDGNIVTNDHVISLGGLVEPDNITVT
                     LADGETFDADVVGRDPFTDLAIIHIDAGEEEIRPLELASSEDVRVGDTVIAIGHALNL
                     QGGPTVTRGVVSAKGRAVTEPGAGPGGTGPTLTDAIQTDAAINSGNSGGPLLDSSGRV
                     IGVNTLVERGNAGGAPVEGIGFAISSDTVRSIAEELIANGQVERGFLGINFTDVPGSF
                     LRRQGIDQDGAVGVVAVEPGSPADSAGLQQGDIITKIGDRVIRNNGDLTAALREFRPG
                     ETVEVEYYRGADRQTAEVTLSDRPEVARPGSDQNIGAI"
     gene            1136..1612
                     /locus_tag="VNL92_06180"
     CDS             1136..1612
                     /locus_tag="VNL92_06180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWO94335.1"
                     /translation="MDEQAKKTALRMIPYGLYILTAKDGQQIASGAINWVTQASFQPP
                     LVVMGVKTDSALYPVVTASRKFALSILGTGQKDLAFAFFKHQEPSEGRLGDYAFETHT
                     TGAPIISDAPAWVEGDVVEIVERGDHAIVIGEVTEAGVKRESAPLTLEECGVKYGG"
     gene            1628..1867
                     /locus_tag="VNL92_06185"
     CDS             1628..1867
                     /locus_tag="VNL92_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94336.1"
                     /translation="MRPPSHDQRKCAFIAPNPNVDRAAIPFERRQGAPLFLCYAKGDP
                     LITTMRKCRYCRDNRLWYKPAPDSPDFVPLASVSE"
BASE COUNT          462 a          753 c          766 g          426 t
ORIGIN      
        1 cgacgacggt gacgcagacg ctgccggctc caccgcgacc gccaccgcca acgggaacga
       61 cgtcaacgga gcttctggca atacggagcc cgacggcctc gagtcctccg ccgagctggt
      121 cgaacaggcg cgcccatcca tcgtgcatat cgaaacggaa gcctccgcgc tcgacatctt
      181 tggtgcgccg gtgccccaga cgggcgtggg tacggggttt atcatcgatg gcgacgggaa
      241 catcgtgacc aatgaccacg taatcagcct tggtggcttg gtcgaacccg acaacatcac
      301 ggtgacgctg gccgacggcg agacgttcga tgctgatgtt gtcggccgcg atcctttcac
      361 cgatctcgcc atcatccaca tcgacgctgg cgaggaggag atcaggccgc tcgaattggc
      421 ctcgagtgag gatgtccgcg tgggggacac ggtcatcgcc atcggacacg cgttgaatct
      481 gcaggggggg cccaccgtga cgcgcggcgt cgtcagcgcg aagggccgcg ccgtcacgga
      541 accgggcgcg gggccgggcg gaacagggcc cacactgaca gatgccatcc agacggacgc
      601 ggcgatcaat tccggcaata gcggaggacc gctgctcgat tcgagcggaa gggtgattgg
      661 ggtcaacacg ctcgtcgaac gtggcaacgc tggaggtgcc ccggtcgagg gcatcggctt
      721 cgccatctct tccgatacgg ttcgctccat cgcggaggag ctcatcgcca acggccaagt
      781 ggagcgcggc ttccttggca tcaacttcac cgacgtgccg ggctcgttcc tgcgaaggca
      841 agggatcgat caggacgggg ccgtgggcgt cgtagccgtc gagcctggct cgcctgccga
      901 cagcgcgggc ttgcagcagg gcgatatcat caccaagatc ggcgacaggg tgatccgcaa
      961 caacggcgac cttaccgcgg cgctgcgcga gttccggccc ggcgaaaccg tcgaggtcga
     1021 atattaccga ggcgccgacc gtcagaccgc ggaggtcacg ttgagcgacc ggcccgaggt
     1081 ggcacggccc gggagcgacc agaatatcgg cgcgatatag actggaggaa gcagcgtgga
     1141 cgagcaagcc aagaagaccg cattgcgcat gatcccgtat gggctctaca ttctcacggc
     1201 gaaggacggt cagcagatag cgagcggcgc catcaactgg gtgacccagg catcgttcca
     1261 gccgcccctc gtcgtcatgg gggtcaagac cgacagcgcc ctgtacccgg tcgtcacggc
     1321 gagccgcaag ttcgcgctga gcatccttgg caccggacaa aaggacctcg cgttcgcctt
     1381 cttcaagcac caggagccga gcgagggcag gctcggcgac tacgctttcg agacgcatac
     1441 cacgggcgcg ccgatcatca gcgacgctcc ggcatgggtc gagggcgatg tcgtcgagat
     1501 cgtcgagcgt ggcgaccacg ccatcgtcat cggcgaggtc accgaggccg gtgtgaaacg
     1561 cgagtcggcg cccctcaccc tcgaggagtg tggcgtcaag tacggaggct gacccgagga
     1621 gggtcttgtg cggccaccca gccacgatca acggaagtgc gccttcatcg cgccgaaccc
     1681 caacgtcgat cgcgcggcga ttccgttcga gcgccggcag ggggcgccgc tcttcctctg
     1741 ttacgccaag ggcgatccgc tcatcacaac gatgcggaag tgccgctact gtcgcgataa
     1801 tcgcctgtgg tacaagccgg cgcccgacag ccccgacttc gtgccgcttg cgagcgtatc
     1861 ggaatgacga ccatccgcga cgcactgggg caaggcttcg atacccccgc gagcgggcgc
     1921 atgatgtcgt cgcgttacac cgtggactac ctcgttaggg atcgccatac cctctgagca
     1981 ccacaccttg ggctaagcac tgcgcgtcgc cgtagcatct gtccagaagg ttgctgtgcc
     2041 catctgtgca gtacgctggg tactttgcgc aaaattcttc gagtggcatt gcgtcggggc
     2101 tacaaaccgt cggattggct ccgcatactt ctttgacgag atttacttcg accatctcag
     2161 agacggccct ccagctattt acacatcccc cgttctgaca tgctggtaac ttcctgctag
     2221 ggtccttcgg gtcgcggatc aaattacaat aggtcgggaa gcgttcacaa tcctctccct
     2281 gcatcgatag tgaattgccg tgggtactcg gcataatttc ttctgctacg gtaatttctc
     2341 ctgtgatcac gctgagcctg ttgcaggaga aaatatctgg atgcggtttg caggcttcgt
     2401 tgcgtgg
//
LOCUS       DATMBB010000288         5423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_368637, whole genome shotgun sequence.
ACCESSION   DATMBB010000288 DATMBB010000000
VERSION     DATMBB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368637"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VNL92_06190"
     CDS             complement(<1..1363)
                     /locus_tag="VNL92_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HWO94337.1"
                     /translation="MAVFGATEAGEMPESPHCPHLGLASDAASFRMRPSADHRCGRQA
                     GWGPSEAHQRHYCLRGEYRYCPAYRGSGKAGSGARTFRPSALLRRRTAVIVTLAVLLT
                     LAGVWGWRVKVLYDDLQAAQAALRVNAAQLDAEMLDLTPAELASLESNFADAAGRLRS
                     VQDRLDGDPLLGIARHLPWFGGQIEAADRLVLVAADLAEAGIAGTRTLQDAPVDLESS
                     EDASVHVLTLTREHAEDLRLVQGATDRALAAVESLRGESLIGPLDAARDEFVGYGADL
                     RDLHAMASFGLIAPAALGEGSLQRYLVLGMNNGELMPGGGFIGNYAVLGLREGEIDSI
                     SSSNVYDLYDRWQERSPGAYVEPPRPLKTYLLGDEWSWAMGEAAWFPDYPTTARTAQH
                     FLDLAGEGPVEGTIGLTVPAMEALLEVTGPIEVPELDTTFTADDVGLRLSFSVTHDAE
                     GDRM"
     gene            complement(1367..2803)
                     /locus_tag="VNL92_06195"
     CDS             complement(1367..2803)
                     /locus_tag="VNL92_06195"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWO94338.1"
                     /translation="MELRQYLNIARRWWWLAVLAMAVAGSAAYFVSSSRTPVYEAEAT
                     LLVNRGQNPNSPTYEDVLSSQQLVKTYAQMIESRPVLEEARGRLPDGVSLSDLEAGVD
                     GSAIEDTQLLRLTYEDTDPDRAADVANEVARVFVGRVRVAQLGDEADEDASEKPINTV
                     FLAEPAVAPDSPVSPQPERDAVLAAIVGLVVAAGIALLIEYMDDTVKHADDLEQLGIA
                     SLGTVQEVRGKQPEGHWAANILDDDQSVRPVAEDFWVVRANLEFAIAATNAKVIEVVS
                     AEPQEGKTTTTCDVAVALAESGRRVLVVDCDLRKPSVHRFFELPNSVGVSTLFLDSTL
                     SPGRAVRPTRIENLFVLPSGPLPPNPAELLSSQRMMAVIRALAEQCDIVLLDTPPLRN
                     MADATLLAPTTDAFLLVIRSGRHRPGAVAESREAIMRTGRPLLGAVLNRERSRRKLGE
                     YERSYYAQGRLSAAAKRDARSTADSSRF"
     gene            2996..3667
                     /locus_tag="VNL92_06200"
     CDS             2996..3667
                     /locus_tag="VNL92_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94339.1"
                     /translation="MEVVNELMVRGIVVGVFAENCWIIGNRRTGEGICIDPGDQPDEV
                     LALAREMGVSVKYIANSHAHIDHVLGVDGVQAATGARFLLHENDAELLKFGWQQAARM
                     MGLRIERGPPDPNAFLREGDVIDVDGLRLQVIGTPGHTQGSVSFYTEGMLFSGDTLFH
                     GSIGRTDLPGGDYDQEMSSICDKLLTLPDDTIVLPGHMDETTVGFEKEHNPFVRDWLA
                     RRASR"
     gene            3845..>5423
                     /locus_tag="VNL92_06205"
     CDS             3845..>5423
                     /locus_tag="VNL92_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94340.1"
                     /translation="MSDDNYWTRTRRTNRRRFLTAGAGAASAGAIALAIGCDDDDDDE
                     GSGGSTPGGESSRQLPEGFEQARYGGVFRYAIGDPPVTLDIHAHDSPGGHAAVYPNYN
                     ALIREPEDPPGTSLGLQPELAEEWEQPDDTTLVLHVRKGVRFQNVEPTNGRELTTEDV
                     KYTLERALGQHPHSSQVASEFRMRDMLGDVQSIEVTGDDTVTMTLREPYAPLLSNIAF
                     GWLQVIPRDLVEGKGDRNVVRWAVGTGPYIMEGYDTLGDGGTITHVRNPDYWRKGHPF
                     FDKIEQPIITAEETRQALYLNRELDNTFSITANVRDTLAEQMGENIQLFDYPPVFNYK
                     VYFDLSNPDSPWARDERLRHAMYYLLPYDVILQSLSVDCCRGAPMAFALDPWALPEDE
                     VPAAGLPLAEAIGEGVKMLAAAGFPPGTEIPLELSVSQFYGGTFLGEALSGIFGAIKQ
                     QSNGAVNLAVKLKVLELADWFKTVYYGGGRYEGTSHGDWGWAEPDSGLYRYFHPKGVA
                     NNTHTDDARLNQLLEAQR"
BASE COUNT          937 a         1828 c         1768 g          890 t
ORIGIN      
        1 acattcggtc gccctcggcg tcgtgggtaa cggagaacga gagccgcagc cccacgtcgt
       61 ccgctgtgaa cgtcgtgtcc agctccggca cctcgatcgg gccggtcacc tcgaggagcg
      121 cctccatggc ggggacggtc aggccgatgg tcccctccac cggcccctcg cccgcgaggt
      181 cgaggaagtg ctgcgcagtt cttgcagtcg tcgggtagtc cgggaaccat gccgcctcgc
      241 ccatggccca cgaccactca tcgcccaaaa ggtaggtctt caatgggcgg ggcggctcca
      301 cgtaggcgcc aggggaacgc tcttgccagc ggtcatagag gtcgtacacg ttgctcgagg
      361 agatcgagtc gatctctccc tcgcggagcc caagcaccgc gtagttcccg atgaacccgc
      421 ctccgggcat cagctcgccg ttgttcatgc cgagcacgag gtagcgctgc agcgagcctt
      481 cgcccaaggc cgcgggcgcg atgaggccga acgacgccat ggcgtgaagg tcgcggaggt
      541 ccgcgccgta gcccacaaac tcgtcgcggg cggcgtcgag tgggccgatc agcgactcgc
      601 cccgcaggct ctcgacagcc gcgagcgcgc gatccgtcgc cccttgcacc agccgcaggt
      661 cttccgcgtg ctcgcgcgtc aacgtcagca cgtggacgct cgcgtcttcg ctcgattcga
      721 gatcgacggg ggcgtcctgg agcgtgcggg tccccgcgat gccggcctcc gcgaggtccg
      781 cggccacgag caccagccgg tcggccgcct ctatctggcc accgaaccac ggcaagtgcc
      841 gcgcgatgcc aagcagtggg tcgccatcca ggcggtcctg taccgagcgc agccgccccg
      901 ccgcgtcggc gaagttcgat tccagggagg cgagctcggc aggcgtaagg tcgagcattt
      961 cggcgtcgag ctgggccgcg ttcaccctca gcgcggcttg ggccgcctgc aggtcgtcgt
     1021 acagcacttt gacccgccat ccccacacgc ccgcgagcgt gagcaggacg gcgagcgtga
     1081 cgatgacagc ggtgcggcgg cgaaggagtg cactgggccg aaaggtcctc gctccgctgc
     1141 ctgctttgcc gctacctcgg tatgccgggc aatagcgata ctcgccgcgc aggcagtagt
     1201 ggcgctggtg cgcctcgctt gggccccacc ccgcctgtcg cccacagcga tggtcggcgc
     1261 tcggccgcat ccgaaagctc gccgcgtcgg aggccaagcc gagatgcggg caatgcggcg
     1321 attccggcat ttcgccggcc tccgttgcac cgaataccgc catcgcctag aacctgctgc
     1381 tatcggcggt agaccgagca tcgcgcttag cggcagcgct cagtcggcct tgcgcgtagt
     1441 agctacgctc atactcgcca agcttgcggc gggagcgctc gcgattgagc acggcgccga
     1501 gcagcggccg gcccgtccgc atgatcgcct cgcgcgattc ggcgacggcg cccgggcggt
     1561 gacgaccgga gcgaatgacg agcaagaagg catcggtcgt aggtgccagc agcgtcgcat
     1621 cggccatgtt ccgaagcggc ggggtatcga gaagcacgat gtcgcattgc tcggcaagcg
     1681 cgcgaatgac cgccatcatg cgctgcgagg agagcagttc cgcggggttc ggcggcaacg
     1741 ggcccgacgg cagcacaaag aggttctcga tccgcgtggg acgaaccgcc cggccgggcg
     1801 agagcgttga gtcgaggaag agcgtcgaaa cgccgaccga gttcggcaac tcgaagaagc
     1861 ggtgcacgga cggcttacgc aggtcgcagt cgaccacgag gacccgccgg ccgctctcag
     1921 ccagggccac ggccacatcg cacgtggtgg tggtcttgcc ctcctggggc tcggcgctca
     1981 ccacttcgat gaccttcgcg ttcgttgcgg caatcgcgaa ctccaggttc gcccgcacga
     2041 cccagaagtc ctccgcgacc gggcgcaccg attggtcatc gtccaggatg ttcgcggccc
     2101 agtgtccttc cggctgcttt ccgcggacct cctgaaccgt gcccaacgag gcgatcccca
     2161 gctgttcgag gtcgtcggcg tgcttcacgg tgtcgtccat gtattcgatg aggagcgcga
     2221 tgccggcggc cacgaccaag cccacgattg cggcgagcac cgcgtcgcgc tcgggctgcg
     2281 gcgagactgg gctatccgga gcgacggccg gctccgcgag gaacacggtg ttgatcggct
     2341 tctccgacgc gtcctcgtcg gcctcgtctc ccagctgcgc gactcgcacg cgccccacaa
     2401 aaactcgggc cacctcgttc gcaacgtcgg cggcgcggtc cgggtccgta tcctcgtagg
     2461 tcaggcgcag gagttgcgta tcctcgatgg ccgagccgtc gacgcccgct tccaggtcgc
     2521 tcagcgacac gccgtcgggc agccgccccc gggcctcctc gaggacgggc cgggactcga
     2581 tcatctgggc gtacgtcttc acgagctgct ggctgctgag cacgtcctcg taggtcggcg
     2641 agttggggtt ctggcctcga ttcaccagaa gggtcgcctc ggcctcgtag actggggtcc
     2701 ggctgctcga gacgaagtac gcggcgctcc cagccacggc catcgcaagg acggcgagcc
     2761 accaccaacg gcgggcaatg ttgaggtact gtcgtagttc cactggccgg cgggcctccc
     2821 tcaagaggag agtcgaagga ttcgcccgtc cagcgtaggc gggcgccacg gccggccaca
     2881 agacaatttc ctaggtttag gggctggcgc ctacctgttt cagggttcgc cccagcgtcg
     2941 ggacatcgcc gcacgccgct tgacccgctg gggaatgaag gctgatattg gaaccatgga
     3001 agtcgtgaac gagctaatgg tccgcggcat cgtcgttggc gtcttcgccg agaactgctg
     3061 gattatcggc aatcgccgca ccggcgaggg catctgtatc gacccgggcg accagccgga
     3121 cgaggtcctc gccctcgcgc gcgagatggg cgtcagcgtg aagtacatcg cgaactccca
     3181 cgctcacatc gatcacgtcc tcggggtcga tggcgtgcag gcggcgacgg gcgcgcggtt
     3241 ccttctgcac gagaacgatg ctgagttgct gaagttcggc tggcagcagg cggcgcgcat
     3301 gatgggactc cgcatcgagc gcggtccccc cgacccgaac gccttcctcc gcgagggcga
     3361 cgtcatcgac gtcgatggac tgcgtctgca ggtgatcggc acgcccggtc acacgcaggg
     3421 cagcgtcagc ttctacacgg agggcatgct cttcagcggc gacacgctgt tccacggctc
     3481 catcggccgc accgacctgc cggggggcga ttacgaccag gagatgagca gcatctgcga
     3541 caagctgctg acactcccgg acgacaccat cgtgctcccg ggccacatgg acgagacgac
     3601 cgtgggcttc gagaaggagc acaacccctt cgtgcgcgac tggctcgcac ggcgcgcatc
     3661 tcgctgagga cccccacccc cgccctctcg ttcataccca aacgaagggt ccggagcgca
     3721 tcggctgttt cctcctcgcc aatgttttga acgtccgttt gtttgataga cctcctttaa
     3781 tcttgccatt gacatggtct atctcccccg ctagtctgtc gcctaaatgc cgagaggggg
     3841 agccatgtca gacgataact actggacgcg gacccggcgg accaatcgcc gtcgcttcct
     3901 gaccgcaggg gcaggcgcgg catcggccgg cgcaatcgca ctggcgatag gctgtgacga
     3961 cgatgacgac gatgagggct cgggcgggag cacgcctggc ggcgaaagct cccgccagct
     4021 gcccgagggt ttcgagcagg cgcggtacgg aggggtcttt cgctacgcca tcggcgatcc
     4081 tccggtcacg ctcgatatcc acgctcacga ctcgccgggc ggccacgcag cggtgtatcc
     4141 caactacaac gcgctgatcc gagagcccga ggatccgccc ggcacatcgc tcggcctgca
     4201 acccgagctc gccgaggaat gggaacagcc cgatgacacg accctcgtgc ttcacgttcg
     4261 aaagggcgtc cgcttccaaa acgtcgagcc gacgaacggg agggaactga ccaccgagga
     4321 cgtgaagtac accctggagc gggcattggg ccagcacccc cacagttcgc aggtggcgag
     4381 cgagttccgg atgcgcgata tgctcggcga cgttcagagc atcgaggtca caggcgacga
     4441 caccgtcacc atgacgctcc gcgagcccta cgccccgctg ctctcgaaca tcgcattcgg
     4501 ttggctgcag gtcatcccgc gagacctcgt cgagggcaag ggggaccgga acgtcgtccg
     4561 gtgggccgtg ggtacggggc cgtacatcat ggagggctac gacacgctcg gagacggggg
     4621 gacgataaca cacgtccgca acccagacta ctggcggaaa ggacatccct tcttcgacaa
     4681 gatagagcag ccgatcatca ccgcggagga gacgcgccag gctctgtacc tcaatcgaga
     4741 gctagacaac accttcagca tcacggcgaa cgtgcgtgac accctcgctg agcagatggg
     4801 tgagaacatt cagctcttcg actatcctcc ggtgttcaac tacaaggtgt acttcgactt
     4861 gtcgaacccc gattctcctt gggcgcgtga cgagcgtctt cggcacgcga tgtactacct
     4921 gcttccctac gacgtcatcc ttcagtcgct ctcggtggat tgctgtcgtg gcgcgccaat
     4981 ggccttcgcg cttgacccat gggccctgcc ggaggatgag gtccctgcgg ccggtcttcc
     5041 actcgccgaa gcgatcggcg aaggggtgaa gatgctcgcc gctgccggct tccccccggg
     5101 aacggagatc ccgctcgagc tcagcgtgtc ccaattctat ggagggacgt tcctcggcga
     5161 ggccctcagc ggcatcttcg gtgcgatcaa acagcagtcg aacggcgccg ttaacctcgc
     5221 cgtcaagctc aaggtcctcg agctcgcgga ctggttcaag accgtctact acggcggcgg
     5281 ccgctatgag ggaacctcgc atggagattg gggctgggca gagcccgatt ctggcctcta
     5341 ccgttacttc cacccgaagg gcgttgcgaa caacacccat acggacgatg cgaggctcaa
     5401 ccagcttctg gaggcccagc gcc
//
LOCUS       DATMBB010000289         3370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_506121, whole genome shotgun sequence.
ACCESSION   DATMBB010000289 DATMBB010000000
VERSION     DATMBB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506121"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNL92_06210"
     CDS             complement(<1..415)
                     /locus_tag="VNL92_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWO94341.1"
                     /translation="MTALVLFDIDNTLLYTGGAGSAAMRLAFADVFGIPDGFAKVEFQ
                     GRTDRLILRDALRIHGLPDAALDARFDDFRAAYLVHLARLLEEYEGHLKPGLPDLLEC
                     LSRAGLRLGLATGNVREGAFRKLRHYGLDGYFERGG"
     gene            complement(453..791)
                     /locus_tag="VNL92_06215"
     CDS             complement(453..791)
                     /locus_tag="VNL92_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWO94342.1"
                     /translation="MPTKNPQEPFTRISIDEAEEILRGDNVALIDVREPHEYEEGHLP
                     GAKLLPVNSVLQRADELPKDKDLVFVCAKGQRSALAAEMAAALGHTTQLYNIEGGTDA
                     WREAGKPIEK"
     gene            954..2714
                     /gene="acsA"
                     /locus_tag="VNL92_06220"
     CDS             954..2714
                     /gene="acsA"
                     /locus_tag="VNL92_06220"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWO94343.1"
                     /translation="MTTQATYETIEKPRLEAFAVRPNLYDYDAQRASFSWEQIRQELV
                     GLPGGGLNVAHEAIDRHLKTPLRTKTAMLWVGKQGEEELYTFEDMARQSNRFANVLRQ
                     LGLTKGDRVFVFLDRIPELYFAAFGTIKAGCIFGPLFSAFGPEAVLDRLQDSEAKVLL
                     TSPDLKPKIDAIRDQLPALEHVIVVDHRNPEPELGPGEKAWQALMDAAPDSFETVHTD
                     PEDLFIMHYTSGTTGKPKGAAHAQRAVYGHFATGRYVLDLHDDDLYWCTADPGWVTGT
                     SYGMFAPWTNGVTNLIYEGGFRASAWYEVIQKYRVTVWYTAPTAIRMLMKAGDDVPKR
                     YDLSSVRYAMSVGEPLNPEAVVWGERVLGFPFHDNWWQTETGSIMIANYPIMPIRPGS
                     MGRPMLGIEPAIVDDEGTEVPAGQEGNLVLKPGWPSMFKTYWKKEEMYQSRFKNGWYY
                     TGDRARKDEDGYFWFVGRADDVINTSGHLVGPFEVESVLIEHPAVAEAGVIGKPDAMA
                     GEIVKAFVAVKDGFEASDKIRGELMQLARQKLGTVAAPREIDFVPSLPKTRSGKIMRR
                     LLKARELGLPEGDTSTLEDD"
     gene            2761..>3370
                     /locus_tag="VNL92_06225"
     CDS             2761..>3370
                     /locus_tag="VNL92_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO94344.1"
                     /translation="MAETYEFLRVDRAPDGIVTVTFDRPPVNAMSQQAMLEITACFRS
                     FADEKETRVVILTAAGERAFMAGVDLADRPQAPPPPSRMLDSGTVARECFWAVYDCAA
                     PVIGAINGPALGAGLAVASMCDVIIASERAKFALPEIDVGLLGGGTHLFRLVGSQYQL
                     RRMMYTGIRVDAKDMARFGCIEAVVPPDELLPTARALAQDIAK"
BASE COUNT          624 a         1076 c         1097 g          573 t
ORIGIN      
        1 agccgccacg ctcgaagtag ccatcgagcc cgtaatgacg cagcttgcga aacgcgccct
       61 cgcggacgtt gcccgtggcc aagcccaagc gtagacccgc tcgggaaaga cactcgagca
      121 agtccggtag gcctggtttc agatggccct cgtactcttc gagcagccgg gcgaggtgca
      181 cgagataggc tgcacggaag tcgtcgaatc gcgcgtcgag cgcggcatcg gggaggccgt
      241 ggatgcgcag agcgtcccgg aggatgaggc gatcggttcg cccctggaac tcgacctttg
      301 cgaatccatc ggggatgcca aagacgtcgg cgaaggcaag gcgcatcgcc gccgatccgg
      361 cccctccggt atagaggagc gtgttatcga tatcgaacag gacgagggcg gtcacgagtc
      421 tccgtctctt ggccgggtgt gtccatccgg acctacttct cgattggctt gcccgcctcc
      481 cgccatgcat cggtaccgcc ttcgatgttg tagagctgcg tcgtgtggcc aagggctgcg
      541 gccatctccg ccgccaaggc ggaccgctgc cccttcgcgc aaacgaagac gagatccttg
      601 tccttcggca gctcatcggc tcgctggagc acgctgttca ccggaagaag cttggctccc
      661 gggaggtggc cctcctcata ctcgtgcggc tcgcggacat cgatgagggc gacattatcg
      721 ccacggagaa tctcctccgc ctcatcgatg ctgatgcgcg tgaacggctc ctggggattc
      781 ttggtcggca tggcgtcgct cccaaatcgt ctcatcggca ttctagcatg gcggcgagtt
      841 gaggctgcct cgccgagcga gatggtcgcg ttgacgggcc tcgaatcggt gagctagagt
      901 gagcgcgttc ccagctcgat ctcgcgttcg agcaccttcg cgaggaggga agtatgacga
      961 cgcaagccac ttatgagacc atcgagaagc cccggctcga ggcgttcgcg gttcggccca
     1021 acctctacga ctacgatgcg caacgcgcgt ccttctcatg ggaacagatc cggcaagagc
     1081 tcgtcgggct ccccggtggc ggactaaacg tggcgcacga ggccatcgac cgccacctca
     1141 agaccccact ccgcaccaag acggcaatgc tgtgggtggg caagcaaggg gaggaggagc
     1201 tctacacgtt cgaggacatg gcgcggcaat cgaaccgctt cgccaacgtc ctgcgccaac
     1261 tcggcctgac gaagggcgat cgcgtcttcg tgttcctcga ccgcataccg gagctgtatt
     1321 tcgccgcgtt tggcaccatc aaggctggat gcatcttcgg gccgctcttc tccgcgtttg
     1381 ggccggaggc ggtgctcgac cgtctccaag acagcgaggc caaggtgctg ctcacgagcc
     1441 ccgacctgaa gccgaaaatc gacgcgattc gcgaccagct tcccgcgctc gaacacgtga
     1501 tcgtcgtcga ccatcgcaat cccgaaccgg agctcgggcc gggcgagaag gcgtggcagg
     1561 ccctgatgga cgcggctccg gactcgttcg aaaccgtcca tacggatccg gaagacctgt
     1621 tcatcatgca ctacacatcc ggcacaacgg ggaagcccaa gggcgcggct cacgcccagc
     1681 gagctgtcta cggtcacttc gccaccgggc gatacgtgct ggacctccac gacgatgacc
     1741 tctactggtg cacagccgac ccaggctggg taaccggcac gtcatacggc atgttcgctc
     1801 cctggacgaa tggtgtcacg aacctcatct acgagggagg cttccgcgcc tcggcttggt
     1861 acgaggtaat ccagaagtac cgcgtgacgg tctggtacac ggcccccacg gccattcgca
     1921 tgctgatgaa ggccggggac gacgtgccga agcgatacga tctgtcatcg gttcgctatg
     1981 ccatgagtgt cggcgagccg ctgaaccctg aggccgtggt ctggggcgag cgcgtgctcg
     2041 gcttcccctt ccacgataac tggtggcaga cggagaccgg ctcgatcatg atcgccaact
     2101 accccatcat gcccatccgg cccggcagca tgggccggcc catgctggga atcgagcccg
     2161 ccatcgtcga cgacgaggga accgaggtgc cagccggtca ggaggggaac ctcgttctca
     2221 agcccggctg gccatccatg ttcaagacgt actggaaaaa ggaggagatg taccagtcgc
     2281 gattcaagaa cggttggtac tacacgggcg accgggctcg caaggacgag gacggctact
     2341 tctggttcgt cgggcgcgcc gatgacgtga tcaacacatc cggccatctg gtcggcccat
     2401 tcgaggtcga gagcgtgctc atcgagcacc cggccgtcgc ggaggcggga gtcatcggca
     2461 aaccggacgc gatggcgggc gagatcgtga aggcgttcgt cgcggtgaag gacgggttcg
     2521 aggcgagcga caagatccga ggcgagctga tgcaactcgc acgccagaag ctcggtaccg
     2581 tcgctgctcc gcgagagatc gacttcgtgc cgtcgctccc gaagacgcga tccggcaaga
     2641 tcatgcgccg cctgctcaag gcccgcgagc tcggcctgcc cgaaggcgac acatcgacgc
     2701 tcgaggacga ttagcatcga ggcggcgtgc ctaaacattg ccggctgaga gggggacggc
     2761 gtggccgaaa cgtacgagtt cctgcgagta gaccgcgcac cggacggtat cgtcaccgtg
     2821 acgttcgacc ggccgccggt aaacgcgatg agccagcagg cgatgctgga gatcacggcc
     2881 tgcttccgat ccttcgcgga tgaaaaggag acgcgtgtcg tcatcctcac cgcggccggc
     2941 gagcgggcgt tcatggcggg cgtcgacctg gctgatcggc cgcaggcgcc gcccccaccg
     3001 tcgcggatgc tcgactccgg cacggtggcg cgcgagtgtt tctgggccgt gtacgattgc
     3061 gccgcgccgg tcatcggggc catcaacggt cccgcgctcg gtgcagggtt ggccgtcgca
     3121 tcgatgtgcg acgtcatcat cgcctcggag agggcgaagt tcgccttgcc ggagatcgac
     3181 gttggcctgc tcggcggcgg cacgcatctg ttccggctcg tcggctcgca gtaccaattg
     3241 cgccgcatga tgtacactgg cattcgggtg gacgcaaagg acatggcacg tttcggctgt
     3301 atcgaagccg tcgtcccccc agacgagctc ctgccgaccg cgcgcgccct cgcacaggac
     3361 atcgcgaaga
//
LOCUS       DATMBB010000290         3286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_8308, whole genome shotgun sequence.
ACCESSION   DATMBB010000290 DATMBB010000000
VERSION     DATMBB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8308"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..741)
                     /gene="hisA"
                     /locus_tag="VNL92_06230"
     CDS             complement(16..741)
                     /gene="hisA"
                     /locus_tag="VNL92_06230"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWO94345.1"
                     /translation="MQIIPAIDILAGRCVRLYQGDYSQETVFAEDPVEVALHWERLGA
                     ERLHVVDLDGARSGRPANREAVRDIIAAVHIPVQTGGGIRTAQDVREYIRSGAARVVI
                     GTSAVQDPRMVGEATQQFPEGIVVSIDARNGVVTTEGWTQTTRERADWFIDHMVEWGV
                     RRFIYTDVTRDGTLTEPNYEAIEALLRKVDVPIIAAGGIARLEHLARLADLGVEAAIV
                     GRALYTGDIDLVRALDAMRAQSA"
     gene            complement(751..1395)
                     /gene="hisH"
                     /locus_tag="VNL92_06235"
     CDS             complement(751..1395)
                     /gene="hisH"
                     /locus_tag="VNL92_06235"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWO94346.1"
                     /translation="MKIAIIDYGAGNLRSVAKAVERVGFEPRVTSDPADACAADAVIL
                     PGVGAAEDTMRNLGEHGLVEPVQQIVAGGTPFLGVCMGMQALLTVSEEGGEHACLDII
                     AGRVRRLPAGVKVPHMGWNTVRQVREHAIFEGIDDGEYFYFVHSYYVDPEDAAVIAGE
                     TEYGVSFPSVLVRGNLVATQFHPEKSGEAGLRIYANFLRAAREANRGAAPVPLG"
     gene            complement(1415..1735)
                     /locus_tag="VNL92_06240"
     CDS             complement(1415..1735)
                     /locus_tag="VNL92_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWO94347.1"
                     /translation="MTRVRVEPAGIEFEAAQGETIMAAAHAAGYYWPTTCGGQAICTT
                     CALVVVEGQDLVDSMGRSERKALTEGRTAAAAQRYRLACQARIAGAGAVVIEKRGVRR
                     SDSA"
     gene            1825..2193
                     /locus_tag="VNL92_06245"
     CDS             1825..2193
                     /locus_tag="VNL92_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWO94348.1"
                     /translation="MVKASLNPGLSAQKTVTTTNEMSAGHLPVYSTPSMIGLMEHAAR
                     DLLQEHLDDGETSVGYRVDVRHLAATPIGKQVTVTATLRELIDGRKAIFDVEAYNEDG
                     VKIGDGMHERRIIPMARMGG"
     gene            complement(2190..2507)
                     /locus_tag="VNL92_06250"
     CDS             complement(2190..2507)
                     /locus_tag="VNL92_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94349.1"
                     /translation="MAAIPEWFDEALMDEASEWISVMLEEEEGAFIDSGLVFQILERE
                     WLMRDDQAAPIPHAEMASMLSERLEADGIRGVPDEISVGLIIGVLTWEDEFLALAGRP
                     RTL"
     gene            complement(2553..2924)
                     /locus_tag="VNL92_06255"
     CDS             complement(2553..2924)
                     /locus_tag="VNL92_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HWO94350.1"
                     /translation="MDDTVREALANDRLIDITTTGRKTGEPRRIEIMFMYDGQDMYLT
                     GRPGRRGWYANLLAQPSFTVHLKQSLQRDIPALAHPIRGEAERRAFFEGVASRWPADQ
                     GEFEMDKWLARSPLVRVELLS"
     gene            complement(3007..>3286)
                     /locus_tag="VNL92_06260"
     CDS             complement(3007..>3286)
                     /locus_tag="VNL92_06260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HWO94351.1"
                     /translation="TIAGLQAARELGARHVELRLDSELIVRQLEGRYRVRKAELRPRF
                     ERVMALIREFDTFAVRHVRREQNREADALANLALDGFPPDAHDSALEG"
BASE COUNT          591 a         1151 c          986 g          558 t
ORIGIN      
        1 ccgtcctccg cttagctacg ctgactgggc gcgcatcgca tcgagcgccc ttacgaggtc
       61 gatgtctcct gtgtagagtg cccttccgac gatggccgcc tcgacgccga ggtccgccaa
      121 tctcgccaag tgctcgagcc gcgcgatgcc acccgcagcg atgatgggca cgtcgacctt
      181 gcgaaggagc gcctcgatcg cctcgtagtt cggctccgtg agcgtgccat ctcgtgtaac
      241 gtcggtatag atgaaccgcc gcacgcccca ctcgaccatg tggtcgatga accagtcggc
      301 ccgctcccgg gtcgtctgcg tccagccctc cgtcgtcacc acgccgttgc gtgcgtcgat
      361 cgagacgacg atgccctccg ggaactgctg ggtcgcctcg ccgaccatcc gagggtcctg
      421 cacggccgac gtgccgatga cgacccgcgc cgcgccggac cgtatgtact cgcgcacatc
      481 ctgggctgtc cgtatcccac cacccgtctg cacagggatg tgcaccgcgg caatgatatc
      541 gcggacggcc tcgcggttcg caggccgccc ggaacgcgcg ccatcgaggt cgacaacgtg
      601 gagccgctcc gcacccagcc gttcccagtg gagcgcgact tcgaccgggt cctccgcaaa
      661 tacggtctcc tggctgtaat cgccctgata caggcgcaca cagcgtcccg cgaggatatc
      721 gatggcgggg atgatctgca acagattcgc tcagccgagg ggcactgggg ccgcgcctcg
      781 attggcttct cgggcggccc gaaggaagtt ggcatagatg cgaaggccgg cctcgccgct
      841 cttctcggga tggaactgcg tcgcgacgag gttccctcgc accaggacgc tcgggaagga
      901 tacgccgtat tccgtctcgc cggcgatcac agctgcatcc tctgggtcga cataatagct
      961 atgcacgaaa tagaagtact ccccgtcgtc gattccctcg aagatggcgt gttcgcgcac
     1021 ttggcgtacc gtattccagc ccatgtgagg gaccttcacc ccggcgggca gccgccggac
     1081 acggccagca atgatatcga ggcaggcgtg ctccccgcct tcctcgctca ccgtgagaag
     1141 ggcctgcatg cccatgcaca cgccgaggaa cggcgttccg cccgcgacga tctgctgcac
     1201 cggctcaacc aatccgtgtt cgccgagatt gcgcatggtg tcctcggcgg cgcccacgcc
     1261 tggcagaatc acggcatctg cggcgcacgc atcggccggg tcgctcgtca cgcgaggctc
     1321 gaagccgacg cgttcgacgg ccttcgccac gctgcgcaag ttccctgcac cgtagtcgat
     1381 gatcgcgatc ttcatgcgca actgcccgcc caacctatgc cgaatcggag cgcctcacgc
     1441 cccgcttctc gatgaccacg gcgccggcgc cggcaatacg ggcctgacac gccagacgat
     1501 acctctgcgc cgcggccgcg gtgcgaccct ccgtaagggc cttccgctcg ctgcggccca
     1561 tcgaatccac cagatcctgc ccttcgacca ccacgagagc gcacgtcgtg cagattgcct
     1621 gaccgccgca cgtcgtgggc cagtagtagc cggcggcatg ggccgctgcc atgatcgtct
     1681 cgccctgggc agcctcgaac tcgatgcccg ccggctccac acgaactctc gtcataatgg
     1741 catgatacct gatcgctcag cccaggtacg agtgcgctat actggcgcgc tgaagcactc
     1801 gccgccctgg aaggagatga caggatggta aaggccagcc tgaatccggg cctatcggcc
     1861 cagaaaacgg tgaccaccac caacgagatg agcgcgggtc acctgccggt ctactcgaca
     1921 ccttcgatga tcggcctcat ggaacacgcc gcgcgtgatc tgctgcagga gcacctggac
     1981 gacggcgaaa cgtccgtcgg ctatcgcgtc gacgtgcgac acctggccgc cacgccgatc
     2041 gggaaacagg tgacggtgac tgcgacactg cgcgagctca tcgatggtcg caaggccatc
     2101 ttcgacgtcg aagcctacaa cgaggacggc gttaagattg gtgatgggat gcacgaacgc
     2161 cgcatcatcc ccatggcgcg catgggcggt tagagcgtcc gcggccgccc cgcgagggca
     2221 aggaactcat cctcccacgt caggacaccg atgatgagcc cgactgagat ctcgtccggg
     2281 acaccacgta tcccatcggc ctcgaggcgc tcggagagca tcgacgccat ctccgcgtga
     2341 gggataggcg cggcttggtc gtcgcgcatg agccattcgc gttcgaggat ctgaaacacg
     2401 aggccagaat cgatgaatgc gccctcttcc tcctccagca tcaccgaaat ccattcggag
     2461 gcctcgtcca tcagggcctc atcgaaccat tcaggaatgg cggccatcgt cccctctcat
     2521 cggcccattg gcggcctgac agcaacgaac gttcacgaaa gaagctcgac gcggaccagc
     2581 gggctacggg cgagccactt gtccatctca aattcgccct ggtcggcggg ccatcgactc
     2641 gccacaccct cgaaaaacgc gcgccgctcg gcctcgcccc gaatgggatg agcaagcgct
     2701 gggatgtcgc gctgcaagct ctgcttcagg tggacggtaa agctgggctg ggcgaggaga
     2761 ttcgcgtacc atccccggcg cccgggccgg ccggtcagat acatgtcttg cccgtcgtac
     2821 atgaacatga tctcgattcg ccgcggctcg ccggtcttgc gtccagtcgt cgtgatgtcg
     2881 atgagccggt cgttcgccaa tgcctcgcga acggtgtcgt ccattcccca tacctccagc
     2941 atcgcctggg cgccggcttg gtcgcgccag tcctatcgta gcgcccgcga gccctcgcct
     3001 ggaagatcaa ccctctaggg cggagtcgtg agcatccggc gggaagccat cgagggccag
     3061 attagcgagt gcatcagcct cgcggttctg ctcccgccgc acgtgacgca cagcgaacgt
     3121 atcgaactcg cggatgagcg ccatcacccg ctcgaatctt ggccgcagct cagccttgcg
     3181 gacacgatag cggccctcga gctggcgaac aatcagctcc gaatcgagac ggagctcaac
     3241 gtgtctcgcc cccaactccc gcgccgcctg aagccctgcg atcgtc
//
LOCUS       DATMBB010000291         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_289432, whole genome shotgun sequence.
ACCESSION   DATMBB010000291 DATMBB010000000
VERSION     DATMBB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289432"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VNL92_06265"
     CDS             <1..374
                     /locus_tag="VNL92_06265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWO94352.1"
                     /translation="SAAVRASVEVESNVPWLDEEVGAGLFQVTQEALSNIRKHAHASR
                     VHTTLGVRGDRVRLTIRDDGVGFDPGAPRSEAHRGLRNMRARVENFGGEVRIESAPNE
                     GTAIIIEVPASAGRPTEVASH"
     gene            484..1308
                     /locus_tag="VNL92_06270"
     CDS             484..1308
                     /locus_tag="VNL92_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWO94353.1"
                     /translation="MPRSIWNGLITFGMVSIPVKLFVATESKDVSFNQLHAACHSRLK
                     QLRWCPVCEREVGWDEIVRGYEYAKDQYVVLTDEDFEKLPLPSQRVIELSAFVNAEEI
                     DPVYYEKSYYLEPDEAGVKPFALLMRALRDKGLTAIAKIAIRNKERLCALRPFDGTLM
                     LETLYYPDELRVQKGVELPAIEVSERELHMAYQLIDLLAEPFDPSKYRDEYRAALMEI
                     IEAKIAGQEVVAPPPPAEAKVTDLMAALQASVEAAMKRKEAELVEGAARKRRRDVA"
     gene            1330..>2207
                     /locus_tag="VNL92_06275"
     CDS             1330..>2207
                     /locus_tag="VNL92_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HWO94354.1"
                     /translation="MTTPLEEYGRKRRFDRTPEPPPAEPPSPRGPLVFVVQKHRARRL
                     HYDFRLEVDGVLVSWSVPKGPALDPRERRLAVQVEDHPFDYGTFEGVIPAGQYGAGEV
                     IVWDDGTYSPDEGGFVFDDPEEANLRMREGLQRGKLSFILRGRKLKGSWALVRMSGHQ
                     RDWLLIKHQDAFATEDRDVLADGRSVRSGLTIEDLQAGRRAEPSPDAIRAPQEAAGTR
                     SAPFPNAVPPMLASLAGSPFSHPEWWFEPKLDGIRALAYLRNGAVRLLSRRGNDITRQ
                     YPALVRALRAQPANAMV"
BASE COUNT          387 a          711 c          750 g          359 t
ORIGIN      
        1 ccagcgccgc tgtgcgggca tcggtggagg tggagtcgaa tgtcccttgg ttggatgaag
       61 aggtcggggc cgggctcttc caggtgacgc aagaggcgct gagcaacatc cgaaagcatg
      121 cgcacgcatc acgtgttcac acgaccctgg gcgttcgggg ggaccgtgtc aggctcacca
      181 tccgcgacga cggcgtaggg ttcgatcctg gcgcgcctcg gtcggaagcg caccgcggcc
      241 tccggaacat gcgggcacgg gtcgagaatt tcgggggcga ggtgcgcatt gaaagcgcgc
      301 cgaacgaggg cacagcgatc atcatcgagg tcccggcgtc cgccgggcgg cctaccgagg
      361 tggcttcgca ttgagcgccc gctgcttcgg acgccccgct gattccgcat cgttgtatga
      421 tggggacgcc ggggtcgccc gatcgtcgtg cggacggcct cgtctgagaa agggagcgct
      481 gcgatgcccc gatcgatctg gaacgggctc atcaccttcg ggatggtgag cattcccgtc
      541 aagctgttcg tcgcgacaga gagcaaggac gttagcttca accagttgca cgctgcctgt
      601 cacagccggc tcaagcagtt gcgctggtgc ccggtctgcg agcgcgaggt gggctgggac
      661 gagatcgtcc ggggctacga gtacgcgaag gatcagtacg tcgtgctcac cgacgaggat
      721 ttcgagaagc tgcccttgcc cagtcagcga gtcatcgagc tctcggcctt cgtgaacgcg
      781 gaggagatag accccgtcta ttacgaaaag agctactacc tcgaaccgga tgaagccggc
      841 gtcaaaccgt tcgcgctcct gatgcgggct ctcagagaca aggggttgac cgcgatcgca
      901 aagatcgcca tccgcaacaa ggaacgcctg tgcgcgctgc gaccgttcga tggcaccctc
      961 atgctcgaaa ccctctacta ccccgatgag cttcgggtac agaaaggggt cgaacttccg
     1021 gccatcgaag tctccgagcg cgagctccac atggcctatc agctcatcga cctgctcgcc
     1081 gagcccttcg acccatcgaa gtatcgtgac gagtatcgcg cagccctgat ggagatcatc
     1141 gaggccaaga tcgcgggaca ggaggtggtc gcgcctccgc cgccagccga ggcgaaggtg
     1201 acggatctca tggccgcgct gcaggccagc gtcgaggctg cgatgaaacg caaggaggcc
     1261 gagctcgtcg agggcgcggc cagaaagcgc cgtcgcgacg tcgcgtgagc gctccgctcg
     1321 cccgttccga tgacaacgcc gctcgaggaa tacggacgca agcggcggtt cgaccgtacg
     1381 cctgagccgc cgccggcgga accgccttcc ccgcgcggcc ccctcgtgtt cgtcgtgcaa
     1441 aagcaccgcg cgcgacgatt gcactacgac ttccggctcg aagtcgacgg cgtgctcgtg
     1501 tcgtggtcgg ttccgaaggg tcccgcgttg gatccccggg agcgccgcct cgccgtacag
     1561 gtcgaagacc acccgttcga ttacggaacg ttcgaaggag tgattccggc cggtcagtat
     1621 ggcgccggcg aggtcattgt gtgggacgat ggcacgtact cgcccgacga aggcggattc
     1681 gtcttcgacg acccggaaga ggcgaatctg cgcatgcggg agggcctgca gcggggcaag
     1741 ctctcgttca tcctccgagg gcggaagctc aaaggttcgt gggcgctcgt tcgcatgagc
     1801 ggacaccagc gtgactggtt gctcatcaag caccaggatg cgttcgccac ggaggaccgc
     1861 gacgtgctgg ccgacggccg ttccgtgcgt tccgggctga ccatcgaaga cctccaggcg
     1921 gggcgccgtg ccgagccctc gcccgacgcg atccgcgcgc cgcaggaggc ggcggggacg
     1981 cggagcgcgc cgtttccgaa cgccgtgcct cccatgctcg cctcgctggc cggcagcccc
     2041 ttctcgcatc cggagtggtg gttcgaaccg aagctcgacg gcatccgagc gctggcgtac
     2101 ctgcgaaacg gcgccgtccg gctgctgtcc cgccggggca acgacatcac ccgtcagtat
     2161 ccggcgctgg tccgcgcgct ccgagcgcag cccgcgaacg cgatggt
//
LOCUS       DATMBB010000292         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_527969, whole genome shotgun sequence.
ACCESSION   DATMBB010000292 DATMBB010000000
VERSION     DATMBB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527969"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="VNL92_06280"
     CDS             complement(<1..1438)
                     /locus_tag="VNL92_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWO94355.1"
                     /translation="MSFGAWKAESKAQCLVNRHRRSAATGVLLNSGVADLWAKRRQEA
                     AKLVEPLALRMRPRTLEEFEGQEHVLGPGRLLRRMIEADTITSLILHGPPGTGKTTLA
                     ELIALRTRRHFVREHAAGVGVKRIREVVDQAAERLSLEGRRTILFLDEIHRFTRAQQD
                     VLLPDVEKGLITLIGATTENPLFAVNAALVSRSTLIRLDPLGEEAVIRVLRRAIADKE
                     RGFGGLSLRVDDEALRVWAVKCDGDARRALTALEVAVLSTMRENGPKSGQDVPIVIGR
                     QAAEDSIQQKAAVYDATGDEHYDVISAFIKSVRGSDPDAAIYWLARMLDAGEDPRFIA
                     RRLAILASEDIGNADPRGIMIAAACWELVERIGMPEARITLAQCTTYLALAPKSNASY
                     MAIDEAMSDVREGRTMPVPMYIKDTNVRKAAGMSEGGEHGPGAGQQYVYAHDEPDAIG
                     RQEYLGVDKSYYRPTDRGAEKLLADMLRR"
     gene            1478..2224
                     /locus_tag="VNL92_06285"
     CDS             1478..2224
                     /locus_tag="VNL92_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWO94356.1"
                     /translation="MAEAAWYNVLWLAWALLCEALRNNPRGDATTGLVYHALRIYAFA
                     FHRLRVAGMEHVPLDRRPGPLVVVCNHTAGIDPILVSAAVPFEIRWMMGRDMMRTDLR
                     DIWEWLRIIPVNRKGRDFAGVREAIRHLSEGGVIGVFPEGGLERPPRRLRPFRHGVGF
                     LISRSRARVLPVWITGTPQIDPAWASLRKPSRSRITFGPVMDFAGRSPEEITAEIEAW
                     FARTSGWPRRTESERPSALPILKGGDRGVG"
     gene            complement(2299..>2908)
                     /locus_tag="VNL92_06290"
     CDS             complement(2299..>2908)
                     /locus_tag="VNL92_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94357.1"
                     /translation="WGMLGTDDMPDVSTFMARDGVEKPTERDIRAWLDEQPDNPFLLE
                     AAVAAVAARGAAGPEDVDLCERYARARPVDPLPHRLLATFYLTGAGVEMGRGPEAAIP
                     HLEFLDAREQSSPGFAIELARRYMALGQSENALAKAARATRISPYDPSVREHAAAIAI
                     RAGDLAKAERHIQALIVLEPDRPLHKQRLDALRAMMADQTPK"
BASE COUNT          413 a         1033 c          956 g          506 t
ORIGIN      
        1 cccgccgaag catgtcggcg aggagttttt ccgccccgcg gtctgtcggg cggtagtacg
       61 acttgtcaac cccgaggtac tcctgcctgc cgatggcgtc gggctcgtcg tgggcgtaga
      121 cgtactgctg tcccgctccg ggcccgtgct ccccgccctc gctcatgccg gcggccttgc
      181 gcacgttcgt gtccttgatg tacatgggga cgggcatcgt gcgcccctcg cgcacgtccg
      241 acatcgcctc gtcgatggcc atgtaactcg cgttgctctt cggcgccagc gcgaggtacg
      301 tcgtgcactg cgcaagcgtg atgcgggctt ccggcatccc gatgcgctcg acgagttccc
      361 agcaggccgc ggcgatcatg atcccgcgcg ggtcggcgtt gccgatgtcc tcgctggcga
      421 gaatggcaag ccggcgggcg atgaatcgag ggtcctcgcc cgcgtcgagc atgcgcgcaa
      481 gccagtagat cgcggcgtca gggtcgctgc cgcgcacgct cttgatgaac gccgagatga
      541 cgtcgtagtg ctcatcgccc gtggcgtcgt acaccgccgc cttctgctgg atcgaatcct
      601 ccgcggcctg ccgaccgatg acgatgggca catcctgccc gctcttcggt ccattctccc
      661 gcatggtgct cagcaccgcc acctcgagcg cggtgagggc gcgccgggca tcgccgtcgc
      721 acttgacggc ccacacccgc agcgcctcgt catccacgcg aagcgacagc cctccaaaac
      781 cacgctcctt gtcggcgatg gcgcgacgca ggacgcggat caccgcctct tcccccagcg
      841 ggtcgaggcg aatgagcgtg ctgcggctga cgagcgcagc gttcacggcg aagagcgggt
      901 tctccgtcgt cgccccgatg agcgtaatga ggcccttctc gacgtccggg agcagcacgt
      961 cctgctgcgc acgggtgaag cgatggatct cgtcgaggaa caggatcgtc cgacgccctt
     1021 cgaggctcag acgctccgcg gcctggtcca ccacctcgcg gatgcgcttg acgcccacgc
     1081 cggccgcgtg ctcgcgcacg aagtgccgcc gcgttcgcaa cgcgatgagt tccgcgagcg
     1141 tcgtctttcc ggttcccggc gggccgtgga ggataaggct ggtgatcgtg tcagcctcga
     1201 tcatcctgcg gagcaggcgc cccggcccga ggacgtgctc ctggccctcg aactcctcga
     1261 gcgtgcgcgg gcgcatgcgg agagcgagcg gctcgaccag tttggccgcc tcctgcctgc
     1321 gcttggccca caggtccgcc acgccggagt ttaggagaac gccggtggca gcggagcggc
     1381 gatgcctgtt gaccaggcat tgcgctttgc tctccgcttt ccatgctccg aaactcacac
     1441 cccgccaccc tcccctctct gataccatcg tcgcctcatg gcggaggcgg cctggtacaa
     1501 cgtgctgtgg ctcgcgtggg cgctgctctg cgaagcactg cgcaacaacc cccgcggcga
     1561 cgcgacgacc ggcctcgtct accatgcgct gcgaatctac gcgttcgcgt ttcaccgcct
     1621 gcgcgtcgcg gggatggagc acgtgccgct cgaccgccgc cccgggcccc ttgtggtcgt
     1681 ctgcaaccac acggcgggca tcgacccgat cctcgtctcc gcggccgtgc cgttcgagat
     1741 ccgctggatg atgggaaggg acatgatgcg caccgacctg cgggacatct gggagtggct
     1801 acggatcatc cccgtcaacc gcaaggggcg cgacttcgcc ggcgtacgcg aggccattcg
     1861 gcatctttcc gagggcggcg tcatcggcgt gtttcccgaa ggtggcctcg agcgaccacc
     1921 gcgccgtctt cgcccgtttc ggcacggcgt aggatttctc atctcccgca gccgcgctcg
     1981 cgttcttccc gtctggatca ccggcacgcc gcagatcgac cccgcatggg caagtctccg
     2041 caagccgagc cggtcgcgga ttacgttcgg tcccgtgatg gacttcgctg gacgatcgcc
     2101 ggaggagatc accgcggaga tcgaggcgtg gttcgcgcgc acgtcgggat ggccgcgacg
     2161 caccgagagc gagcggcctt cggcactccc catcctcaag ggaggggacc ggggggtggg
     2221 ctgagaaccg ctccatgttt cgttacatcc cgcacccgat tgtcggccta tggctggcgc
     2281 gtttcagccc acgcgggctc acttcggcgt ctgatccgcc atcatcgcgc gcaaggcgtc
     2341 gaggcgctgc ttgtgcaggg ggcgatccgg ctcaagaacg atcagcgcct ggatgtggcg
     2401 ctcggccttg gcgagatcgc ccgcccggat cgcaatggct gcggcgtgct ctcgcaccga
     2461 cggatcgtac ggagagatgc gcgtcgcccg ggcggccttt gccagcgcat tctcggactg
     2521 cccgagggcc atgtaccggc gagccagttc gatggcgaag ccgggtgaag actgttccct
     2581 cgcgtcgagg aactcaaggt gcgggatcgc cgcctccggc cctcgcccca tctcgactcc
     2641 cgcgccggtg aggtaaaacg tcgccaacaa gcggtgcggc agcgggtcaa cggggcgcgc
     2701 ccgtgcgtac cgctcgcaga ggtccacgtc ctccggcccg gccgccccgc gcgccgcgac
     2761 cgccgccacc gctgcttcga gaaggaaggg gttatccggt tgctcgtcca gccatgcgcg
     2821 gatatcccgc tccgtcggtt tttccacccc gtcgcgggcc atgaacgtgc tcacgtcggg
     2881 catgtcgtcc gttccgagca tgccccag
//
LOCUS       DATMBB010000293         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_174540, whole genome shotgun sequence.
ACCESSION   DATMBB010000293 DATMBB010000000
VERSION     DATMBB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174540"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VNL92_06295"
     CDS             complement(<1..485)
                     /locus_tag="VNL92_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94358.1"
                     /translation="MLLVTSASLLAGVAVFLVLSSLLVPSTRLLDRLPQAAAVAASTG
                     KADRPAPFADRVVAPVVAALSETLGRVLPARIRERTQDQLEMAGRNRTDWTKFVALQV
                     VAGVVLAGLALLMPFSAGLKVLFVPLLLFLGYRLPSFFVARSISKRNRQIERALPDAV
                     DL"
     gene            complement(488..1456)
                     /locus_tag="VNL92_06300"
     CDS             complement(488..1456)
                     /locus_tag="VNL92_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWO94359.1"
                     /translation="MVTALAAGAAVLAFIAVVLPVAALSADAHSPARARLSGEWRSPL
                     PAEKPSSGRVLLRRGPTTHLTVVGWVLQRRQLGTYIQRTLQAAGWTLTPSEFVLLSLV
                     AGGLGGLVTTVLGLTPVLGIAAVAGGALVPWVILRQARARRLKLVHAQLVDAFTLIAG
                     AMRSGSNLTQALEATMRELPEPAREEFLHTLEELQVGATVEEAFGNLGERVQSPDLDL
                     VITAIVIQRQVGGNLAGILETTADTMRQRQRIRGEIKSLTAQGRLSGLIIGLLPVAVF
                     LMLTFINPEYMEPLTTTNTGRLVLGLGVGLEAMGFFVIRRIVSIEV"
     gene            complement(1450..2668)
                     /locus_tag="VNL92_06305"
                     /pseudo
     CDS             complement(1450..2668)
                     /locus_tag="VNL92_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256416.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="CpaF family protein"
BASE COUNT          466 a          895 c          918 g          389 t
ORIGIN      
        1 agatcgacgg cgtcgggcag cgcacgctcg atctgccggt tccgcttcga gatgctgcgc
       61 gcgacgaaga acgacggcag ccggtagccg aggaacagga gcagcggcac gaagagaacc
      121 ttgagacccg ccgagaacgg catgagcaac gccagccctg ccagcaccac gccggcgacg
      181 acctggagcg cgacgaactt cgtccagtcg gtgcggttgc gccctgccat ctcgagctga
      241 tcctgcgtcc gctcgcggat gcgggcgggc agcaccctgc ccagcgtctc cgacagggca
      301 gcgacaacag gcgccacgac gcggtcggcg aacggcgccg ggcggtccgc cttgccggtg
      361 ctcgccgcca cagccgcggc ttgcggcaat cgatcgagca gacgggtcga gggcacgagc
      421 agcgacgaca gcacgaggaa gacggcaacg ccggcaagca gtgacgcgga tgtgaccaac
      481 aacatggcta tacctcgatc gaaacgatgc gccgtatgac gaagaagccc atcgcctcga
      541 gtccgacccc aagaccgagg acgaggcgac ccgtattcgt ggtggtcagc ggctccatgt
      601 actccgggtt gatgaacgta agcatcagga acacggccac cggcagcagc ccgatgatga
      661 gacccgagag gcggccttgc gccgtgagcg acttgatctc gccgcggatg cgttgtcgct
      721 gccgcatcgt gtcggccgtc gtctcgagga ttcccgcgag gttgccgccg acctgccgct
      781 ggatgacgat ggcggttatg acgagatcga ggtccgggct ctgcacgcgc tcacccagat
      841 tcccgaacgc ctcctccacg gtggcgccca cttgcagctc ttcgagcgtg tgcaggaact
      901 cctcgcgcgc cggctcgggc agctctcgca tcgtcgcctc gagggcctgg gtaaggttgc
      961 ttccggagcg catcgccccg gcaatgagcg tgaacgcgtc gacgagctgc gcgtgcacga
     1021 gcttcaagcg tcgagcacgg gcctgtcgca ggatgaccca cggcacgagc gctccgccgg
     1081 ccacggcggc gatgccgagc accggtgtca gcccaagcac cgtcgtaacc agcccgccaa
     1141 ggccaccggc taccaagctg agcaggacga actcgctcgg cgtgagcgtc cacccagccg
     1201 cctgcaacgt acgctggatg taggtgccga gttgccgccg ctgcagcacc cagccgacga
     1261 ccgtcaggtg ggtggtagga ccgcggcgga gcagcacacg acccgaggaa ggcttctccg
     1321 caggcagcgg cgagcgccac tcgccgctca ggcgggccct cgcgggcgaa tgcgcatcgg
     1381 cggacagcgc ggccacggga aggacgacag cgatgaacgc gaggacggcc gcgccggccg
     1441 cgagggccgt taccatcgct caccccgcag atcggcgggc acaccgaaca ccgaaaggtc
     1501 gaggttcacg cccagcctgc tgaaggcctc ggcaaacacc gggcgcaggc cggtcggcac
     1561 gtgcgagccg acgacacgtc gtcctcgtcg aagcccgtcg tgtggaagag gaagatctcc
     1621 tgggtcgtaa taatgtcgcc ctccatgccg gtcacctcgg tgatgtgcga gacgcgccgc
     1681 gaaccgtcgg ggaagcggct tacctggacg atgaggtgga tggccgaggc gagctgttcg
     1741 cgaatggctc gtgccggcag gtcgaggttc gccatcagca ccatgttctc cacacgggag
     1801 agcgcgtcgc gcggcgtgtt ggcgtgcacc gtactgatcg acccttcatg gccggtgttc
     1861 atggcctgga gcatgtcgaa cgcctcgccg ccgcgaacct cgccgaggat gatgcggtcc
     1921 gggcgcatac gcagcgagtt gcgcacgagg tcccgctgcg tcacctcgtg ctccccatcg
     1981 acggccggcg gccgcgcctc cagggtgatg acgtgctcct ggcggagctg cagctcgacc
     2041 gggtcctcga tggtcacgat gcgctcgtgc gcgggaatga acgccgagag cgcgttcagc
     2101 agggtcgtct taccggatcc ggtaccgccg gagatcagga tgttcttctt cgccagaacg
     2161 catgcccgca gaaactccgc gatggccggg ctcagcgatc cgatagccgc aaggtcctcg
     2221 atgccgaggc ggtcctggcg gaacttccgg atcgtgatgt tggggtgcct cggcgctacg
     2281 ggcgggatca cggcgttcac gcgggagcca tccggaagcc gcgcatcgac catcggcgac
     2341 gactcgtcga tacggcgccc gatgggcgcc acgatacgct cgatgatgcg gaggatgtga
     2401 tcgttgtcgc ggaacgtgta cggcgctcgc tcgatgcgcc cgtcccgctc gatgtagacg
     2461 tggtccgggc tattcaccat gatttccgag atcgatgggt cccggtagag cgggtcgatg
     2521 gggccgagcc ccagcacctc gtcggccagc tcgagcacga gctgctgccg cacgacgggc
     2581 gagaggccag gcgcctgctc gctgaggacg accttcgcgg ccgcctcgac ctcctcccgc
     2641 gcctgcctcg ggttgcgcga gagcttgc
//
LOCUS       DATMBB010000294         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_90912, whole genome shotgun sequence.
ACCESSION   DATMBB010000294 DATMBB010000000
VERSION     DATMBB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90912"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VNL92_06310"
     CDS             complement(<1..456)
                     /locus_tag="VNL92_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWO94360.1"
                     /translation="MRRDRELEGLPLIKVVGVGGGGSNAVNRMAEHRIAGVELIAVNT
                     DAQALAMSNAPTRIRIGERLTKGLGVGGDPVKGERAAEESREELYETLQNAEMIFITA
                     GMGGGTGTGAAPIVGSIAREIGALSVAVVTLPFGFEGAKRRRLAEQGLAQ"
     gene            complement(527..1768)
                     /gene="ftsA"
                     /locus_tag="VNL92_06315"
     CDS             complement(527..1768)
                     /gene="ftsA"
                     /locus_tag="VNL92_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWO94361.1"
                     /translation="MRTGGTFAAIDLGSTKVCTIVGDLALGPRPRILGVGTAPSHGIS
                     RAMVDNIAEATESIRVSVEKAERASGTRIFSAYVSMSGSHIASLNNRGIVAIPQRDRP
                     ISVDDVERVIEASRTVSIPTNREIVHAVPRYYVVDGQDHVSDPIGMYGQRLDVETHVV
                     TGALSAIQNVRKCVEGSGIQVDDLVLSPMAAALSVLDEEEREQGVVLADIGGGTTDIA
                     IFSEGAVMHTSVLPVGGYHLTRDLVVGLRVPYHAAEQLKREHGNVMTSISEVGVVEVE
                     AFGTERRREVPLRRIGEVLQARAEEMLEMIFMEARRAGYEETLSAGLVLTGGTAGLRG
                     LPELAEQVLGIPARVGVPRHLQGLLEAVDDPALATATGLLEWAVFASAGEVRSPRATS
                     SLDLSGLWRKLGSMARAVLPQ"
     gene            complement(1809..>2635)
                     /locus_tag="VNL92_06320"
     CDS             complement(1809..>2635)
                     /locus_tag="VNL92_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWO94362.1"
                     /translation="LAEDSGLRGKSILTADLDAAEQHLESNPLIADVHIERDWPLDVR
                     IIVTERQAWAVWQTERERYVVDKEGFVLEGIDPPIGAPVIIDAREQNGEPFTRGIADI
                     VTTVYNLRDFMPSVVGMEPRGFRLEDDRGLVVSASDGHVAVFGDGHDFDYKLAVWKAL
                     LDAAEAGDFPSAWAEVVDLRFGDRPSLVRAIGAIEEQEPGDAVEAGVGEESPAATEVP
                     LDEALPEEAEPAAEEPPVSDVGADATAPLPDGTTGVVEAAEPPPGLQPADPASGVE"
BASE COUNT          387 a          940 c          843 g          465 t
ORIGIN      
        1 ctgcgccagg ccttgctcgg ccaaacgacg gcgcttggcg ccctcgaacc cgaagggcag
       61 cgtgaccacg gctacgctga gcgcgccgat ttcccgcgcg atggacccaa cgatgggtgc
      121 cgcgcccgtg ccggttccac ctcccatgcc ggcggtgatg aagatcatct cggcgttttg
      181 aagggtttcg tacagctcct cgcggctctc ctcggccgcg cgctctccct tcaccgggtc
      241 gccgccgacg ccgaggcctt tcgtcagccg ctcgccgatc cggatgcgag tcggcgcgtt
      301 cgacatggcg agcgcctgag catccgtatt gacggcgatg agctcgaccc ctgcaatgcg
      361 gtgctccgcc atgcgattca cggcgttgga gccgcctccg cccactccga cgaccttgat
      421 gagtggcaga ccttcgagtt ccctgtctct ccgcatatcg aacgatctcc ttcctaccct
      481 tgtctccatg accttcccct ttcctcgttc ctggcccact ggccgcctat tgcggtagca
      541 ccgcccgcgc catgctcccc agttttcgcc acaatcctga caagtcgagc gacgaggtgg
      601 cgcgtggcga tcgcacctcg cccgcgctcg cgaagacggc ccattccagt agccccgtag
      661 ccgtcgccag cgccggatcg tcgacggcct cgaggaggcc ttggaggtgc cgcggcacgc
      721 ccacacgagc cgggatgccg agcacctgct cggcgagctc cggcaggcca cgcaggcccg
      781 ccgtcccgcc ggtcagcaca agcccggccg agagtgtctc ctcgtagccg gcccggcgcg
      841 cctccatgaa gatcatctcg agcatctctt cggcgcgggc ctgcagcact tcgcctatac
      901 ggcggagcgg cacctcgcgc cgccgttccg tgccaaacgc ctcgacctcc acgacgccga
      961 cttccgagat cgacgtcatc acgttgccgt gctcgcgctt cagctgctcg gccgcgtggt
     1021 acggaacacg taggcccacg acgagatcgc gcgtgaggtg gtagccgccg accggaagga
     1081 cggaggtgtg catcacggcg ccttcggaga agatggcgat gtccgtcgtg cctcccccaa
     1141 tgtccgcgag cactacgcct tgctcgcgct cctcctcgtc gaggacggac agcgcggccg
     1201 ccatggggct caggaccaag tcgtcgacct ggatgccact cccctccaca cacttcctta
     1261 cgttctggat ggcggagagg gccccggtaa cgacgtgggt ctcgacatcg agccgctggc
     1321 cgtacatgcc gatcgggtcg gacacgtggt cctgtccgtc tacgacgtaa tatctcggga
     1381 cggcatgcac gatctcgcgg ttcgtcggga tgctgaccgt tcgcgatgcc tcgatgaccc
     1441 gttccacgtc gtcgaccgag atggggcggt ctcgctgggg gatggcgacg atgccccggt
     1501 tgttcaacga agcgatgtgg gagccggaca tgctgacgta ggcggagaag atgcgcgttc
     1561 cgctggcccg ctccgccttc tccaccgaga cgcgtatcga ctccgtggct tcggcgatgt
     1621 tgtcgaccat ggcgcggctt atgccgtggg aaggcgcggt gccgacgccc aggatgcgag
     1681 ggcgaggccc cagcgcgagg tcgccgacga tggtgcaaac cttcgtgctg ccgaggtcga
     1741 ttgccgcgaa cgttcccccc gtcctcatgg tttcctcctt cccccggccg ccgcctgcgg
     1801 gcccatggct actccacccc cgaggcgggg tctgctggct ggagtccggg aggcggctcg
     1861 gcggcctcca cgacgcccgt cgtgccatcc ggcagcggag cggtcgcgtc ggccccgaca
     1921 tcgctcacgg gcggctcttc cgccgcgggc tcggcttcct cggggagcgc ctcgtcgagg
     1981 gggacttccg tcgctgccgg cgactcctcc cctacgcccg cctcgaccgc gtcgccgggt
     2041 tcctgctcct cgatggcccc gatggctcgg acgagcgacg gtctatcgcc gaagcgaagg
     2101 tcgacgacct ccgcccacgc cgacgggaag tcgcccgcct cggccgcatc gaggagcgct
     2161 ttccagacgg cgagcttgta gtcgaagtcg tggccgtccc cgaagacggc gacatgtccg
     2221 tccgatgcgc tgacgacaag gccgcggtcg tcctccaggc gaaagcctct gggttccatt
     2281 cccacgacgg agggcatgaa atcgcggagg ttgtagactg tcgtcacaat gtccgcgatg
     2341 ccgcgcgtga acggctcgcc gttctgctcg cgggcatcga tgatgacggg agcgccaatc
     2401 ggaggatcga tgccctcgag cacgaagccc tccttgtcga cgacgtagcg ctcccgttcg
     2461 gtctgccaga ctgcccatgc ttgacgttcg gtgacgatga tgcggacatc gagcggccaa
     2521 tcgcgctcga tgtgcacgtc ggcgatgagc ggattcgatt ccaagtgctg ctcggctgcg
     2581 tccaggtcgg ccgtcagaat gctcttgccg cgcaggcccg agtcctcggc gagaa
//
LOCUS       DATMBB010000295         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_131350, whole genome shotgun sequence.
ACCESSION   DATMBB010000295 DATMBB010000000
VERSION     DATMBB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131350"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2409)
                     /gene="polA"
                     /locus_tag="VNL92_06325"
     CDS             complement(<1..2409)
                     /gene="polA"
                     /locus_tag="VNL92_06325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWO94363.1"
                     /translation="MLASPDEPRLLLLDGHGIIHRAYHAFKEPLVVRRTGEVATAVFG
                     FANTLLKALEDLRPTHIAVALDAPGPTFRHERDEAYKAHREAMPSDLRSQLARVRELI
                     EAFNIPMFELPGWEADDIIGSLARQAAESGMPVYIATLDSDLIQLVRSGVFVYMYRPY
                     QRDAVLYDAERAEERYGIPPEAMPDLKGLKGDASDNIPGVPGIGEKTACKLLQQFGSL
                     DGIYARIDEVTPEKLRQKLIEYRDVAMHSRDMATIDVNTPVALDLEACRVHEFDRGRV
                     LDLFRELEFRSLMSRLPDYEGMAPLEAVTAQQPEAPGTVCHVVRNIEELERLADDLRQ
                     AGCFAFRAEATHHLAMQASLVGIALASREREAYYIPLGHAAMHLGETQLPLAEVARVL
                     GPLFADPAIEKIGHNVKFDMLVLERIGMPTDGVRFDTMIAAHLLGETTLELNTLAYER
                     LGVDMPAMSALLGTGRRQLSLADVSADELSPYACTNADVTLRLREGFGRRLEELQLRP
                     LFADIEMPLVPVLGRMERTGIAVDTAVLRDMSGQLAKQLAELERRIYEEVGHEFMLGS
                     PQQLSDVLYTELGLPKPRKTQRGYTTDAATLEKLRPYHPVIDLLLEHRQYGKIKSTYV
                     DALPGMIHPETGRVHTTFSQTTAATGRLASSDPNLQNIPVRTELGRQVRRAFVARDVG
                     PDPVLLAADYSQIELRILAHLSGDPGLIAAFVNGEDIHRATAARMYSVAPADVTPDMR
                     RIAKVVNFGVVYGLSEFGLTQRTELSREEAATIIGAYFEKYPGIRSYIEQTIDSTRER
                     GYAET"
     gene            complement(2417..>2750)
                     /locus_tag="VNL92_06330"
     CDS             complement(2417..>2750)
                     /locus_tag="VNL92_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO94364.1"
                     /translation="VDRMYDDFNRETRRAVLRLYRASRDIGRYVTAAAEALSPWGIPA
                     LVIWGKRDPYISYLDAQRALGLLPEAEFILLDGSGHWPFVDNPDAVADAIVPFLSRHV
                     RGNEPRVY"
BASE COUNT          472 a          844 c          945 g          489 t
ORIGIN      
        1 cgtctccgcg tagccgcgct cgcgcgtcga atcgatcgtc tgctcgatgt aactgcgtat
       61 acccgggtac ttttcgaagt aggcgccgat gatcgtggcg gcctcttcgc ggctcagctc
      121 cgtgcgctgc gtgaggccga actccgacag cccgtatacc accccgaagt tgacgacctt
      181 cgcgatgcgt cgcatgtccg gcgtgacgtc cgcgggggcg acgctgtaca tgcgggcggc
      241 ggtggctcgg tggatgtcct cgccgttcac gaaggcggcg atgaggccgg gatcgccgga
      301 aaggtgggcg aggatccgaa gctcgatctg cgagtagtcg gcggcgagga gcacgggatc
      361 gggcccgacg tcgcgggcga cgaatgcccg gcggacctgc cggccgagct ccgtgcgtac
      421 gggaatgttc tgcaggttcg gatcgctcga tgcgagccgc cccgtcgccg ccgtcgtctg
      481 gctgaaggtc gtgtgtaccc gaccggtctc ggggtgaatc atgccgggga gcgcatcgac
      541 gtacgtcgac ttgatcttgc cgtactgccg atgctcgagc agcaggtcga tgacggggtg
      601 gtacgggcgc aacttctcga gcgtcgcagc gtcagtcgtg tatccccgct gcgtctttcg
      661 cggtttcgga aggcccagct cggtgtagag gacatcggac agttgctggg gcgaccccag
      721 catgaattcg tgaccgacct cctcatagat gcgccgctcg agctcggcga gctgtttggc
      781 cagctgcccc gacatgtcgc gcaggacggc agtatcgaca gcgatgccgg tgcgctccat
      841 gcggcccaga acagggacca gcggcatctc gatgtccgcg aacaagggcc ggagctgcag
      901 ctcctcgagg cgccgaccga acccctctcg caagcgaagc gtcacgtcgg cgttggtgca
      961 ggcgtaaggg ctgagctcgt cggcggagac atcggcaagg gagagctgcc gccgtcccgt
     1021 gccaagcaac gcgctcatgg ccggcatatc gacgccgagg cgctcgtacg ccagggtatt
     1081 gagctcgagg gtcgtctcgc cgaggaggtg ggcggcgatc atcgtgtcga agcggacacc
     1141 gtccgtgggc attccgatgc gttccagtac gagcatgtcg aacttcacgt tgtgcccgat
     1201 cttctcgatc gccgggtcag cgaacagcgg tcccaggacc cgcgcgacct cggccagcgg
     1261 cagctgcgtt tcgcccagat gcatggcggc atggccaaga gggatgtagt acgcctctcg
     1321 ttcgcggctc gccagggcga tgcccacgag cgaggcctgc atggccaagt ggtgcgtggc
     1381 ctccgctcga aaagcgaagc atcccgcctg acgcaggtcg tcggccaggc gttccagttc
     1441 ctcgatgttt cggacgacgt gacagacggt gccaggagct tccggttgct gagccgtcac
     1501 ggcttccagt ggagccatgc cctcgtaatc gggaaggcgc gacatcaggc tgcggaactc
     1561 gagctctcgg aatagatcga ggacgcggcc acgatcgaat tcgtgcacgc ggcacgcctc
     1621 gaggtcgagc gcgacgggcg tgttcacgtc gatcgtggcc atatcacggc tgtgcattgc
     1681 gacgtcgcgg tactcgatga gcttctgacg cagcttctcc ggcgtcacct catcgatgcg
     1741 cgcatagatt ccgtcgaggc tcccgaactg ctgaaggagc ttgcaggccg tcttctcgcc
     1801 gatgcccggt acgccgggga tattgtcgga ggcgtcgcct ttcaggcctt tgaggtcggg
     1861 catggcctcg ggaggaatgc catagcgctc ctccgcgcgc tcggcgtcgt agaggacggc
     1921 gtcacgctga tagggccggt acatgtacac gaaaacgccg gaccgcacga gctgaatgag
     1981 gtcgctatcg agggtcgcga tgtacacggg catgcccgat tcagctgcct gtcgcgccaa
     2041 ggagccgatg atatcgtcgg cctcccagcc cggcagctcg aacatcggga tgttaaaggc
     2101 ctcgatcagc tcgcggacac gtgccagctg ggaacggagg tcgcttggca tggcctcgcg
     2161 gtgcgccttg taggcctcgt cgcgctcatg acggaaggtc ggtccaggcg cgtcgagggc
     2221 gacggcgatg tgggtgggcc ggagatcttc gagtgccttc agcagcgtgt tcgcgaagcc
     2281 gaataccgct gtcgcgacct cacccgttct ccgaacgacg aggggctctt tgaaagcatg
     2341 gtaggcgcga tgaatgatgc cgtggccatc gagaaggagc aggcgaggtt cgtccggtga
     2401 agcaagcatg atacatctag tatacgcgcg gctcgttgcc cctgacgtgg cgggagagga
     2461 agggcacgat ggcgtccgca acggcgtccg gattgtccac aaagggccag tgtccgctcc
     2521 cgtcgagcaa gatgaactct gcctcaggca acagaccgag cgctcgctgc gcgtccagat
     2581 acgagatgta cgggtcccgc tttccccaga tgacgagcgc cgggatgccc cagggcgaca
     2641 gcgcctcggc tgcggccgtc acgtatcgtc caatgtcacg actcgcgcgg tagagtcgaa
     2701 ggacggcgcg gcgcgtctcc cgattgaagt cgtcatacat gcgatcgacg
//
LOCUS       DATMBB010000296         5170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_288231, whole genome shotgun sequence.
ACCESSION   DATMBB010000296 DATMBB010000000
VERSION     DATMBB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288231"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="glmU"
                     /locus_tag="VNL92_06335"
     CDS             <1..1013
                     /gene="glmU"
                     /locus_tag="VNL92_06335"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWO94365.1"
                     /translation="QAEATLTLLTAEPDDPRGLGRILRTNGRIAAIREEHDASEAERA
                     IREVNAGVYCFDARWLWQSLPNVEPAPNGELYLTALVERAAAEGRKIEVVLAADWEES
                     IGVNDREQLARAEAVYRRRMLRTLMVDCGVTIIDPATTYIDAGVEIAPDTTIEPNTHI
                     RGHTRIGSGCTIGPNAMLRDARIGEGCRVFASCLEDCELEDGVDVGPYSHVRPGAHLE
                     AGVHVGNYVEVKASRIGRDSKIGHFSYIGDALLGANVNIGAGTVTCNFDGERKHATHI
                     GDDAFIGSDTMLVAPVRIGRGARTGAGSVVTHDVAEGETVAGVPARGLRQKRAEGAVD
                     GR"
     gene            1003..2301
                     /locus_tag="VNL92_06340"
     CDS             1003..2301
                     /locus_tag="VNL92_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWO94366.1"
                     /translation="MDAERLLSIALLLVIGVVLGLLSMAEAGAITISRQRARLLAERG
                     ERHGRALHRQAIERAATYASFTVIRSALLIGGTILLAGLLIDALDDDLAALALAYFIA
                     LGVTLLLEIAPGYIAVQQPERWALPFTPVVQAIRVVVGPIAWLFTLPAQVWLRLRGVG
                     GNGGDEVSEAEELLRQVEMERGEEIEREEAEMIRGIFGLEDTTAREVMVPRIDMVAVE
                     AGESVETAVQTIVEKGVSRIPVYKDTIDNIVGIVYAKDLLRYLAAASPPVTLTDLARP
                     AHFIPETKRTDELLRELRQSKVHIAIVVDEYGGTAGLVTIEDLLEEIVGEIEDEYDIA
                     TQLIEVVSEAEAVLDARVPVDELNELFGIEVDHEDYDTVGGFVYHHLGTIPSVGDEIE
                     VDGIHVRILSVEDRRIKKVHMRRVQPPDPGAGAERRERAS"
     gene            2291..3211
                     /locus_tag="VNL92_06345"
     CDS             2291..3211
                     /locus_tag="VNL92_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94367.1"
                     /translation="MPPERGGRTIVSQELPAPAHRVSPVGLPAAYRLPPRPAGEGVDQ
                     QVADARRQSSFVLGEDVRLFERGMNLQMDVLRDSHPVRYRTHALAALVMQWSRAYAYE
                     RDAFELALHGSYISALPLLRAAAECLAAQMGLHRGDLPEFEAWLGGVGLPQSALHAVQ
                     VPLGRYRSSASIADDARLGPIYRATADLANTHFGSSLLQVGPESNQQKLALAFGDRAF
                     HLGWAEVVFGWALGLVSRQLTFACEAHDVLNIGDERRSEVESYASAVAGALERPDRCA
                     IMEIEHEGERRLLVHNFRRAPGSAGRRILL"
     gene            3296..4309
                     /gene="gap"
                     /locus_tag="VNL92_06350"
     CDS             3296..4309
                     /gene="gap"
                     /locus_tag="VNL92_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWO94368.1"
                     /translation="MVTKVGINGFGRIGRQVFKAILERHSDTLEVVAVNDLTDTDTNA
                     HLLKYDSNYGRFPGTVEARDGGLVVNGKDVQVFAERDPAQIPWRNVGADVVVESTGRF
                     TDATKAAGHTQGGAKKVIISAPAKNEDLTVVLGVNEGMYDPAKHTVISNASCTTNCIA
                     PVVKVLHSSFGVEKGLMNTIHSYTNDQVILDTVHDDLRRARAAAINIIPTTTGAAKAV
                     TVVMPELQGKIHGLAFRVPTPTVSVCDFTAELGRQATVDEVNKAFEDAAREALKGILA
                     YETEELVSSDFKENPHSAIFDAPGTIVIGGNMVKVLAWYDNEWGYSCRVADLCDYIAK
                     RGL"
     gene            4405..5166
                     /locus_tag="VNL92_06355"
     CDS             4405..5166
                     /locus_tag="VNL92_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO94369.1"
                     /translation="MQGKVALITGSSRGIGHFMAVELAKAGADIVVAARSEQEPDPRL
                     PGTIHSVAAEISALGRRALPVRMDLTKDEEVEQGVQQAIAEFGRIDVLINNAGIMAPA
                     PLVDLPMKRWDLVMRVNVRGAVVATKAVLPHMMERKSGTIIFISSVAAEMSGAGNISY
                     AVSKLSLEKIAQGLAEEVRDDGISVFGLAPRSLVITPGNSFVRGDARPEGIAIEPPEQ
                     MGQAAIYLCSDDAKQYSGHTFYSDAAAARRGEPVR"
BASE COUNT          947 a         1599 c         1785 g          839 t
ORIGIN      
        1 ggcaggcgga agccacgctt acgctgctga cggccgaacc cgacgatccg cgcggtctcg
       61 gccgcatcct tcggaccaac gggcgcatcg cggctatccg agaggaacat gacgcgagcg
      121 aggcagagcg cgcgatccga gaggtcaatg cgggcgtcta ctgcttcgat gcgcggtggc
      181 tgtggcaatc attgccgaac gtcgagcccg cgccgaacgg ggagctctac ctgacggctc
      241 tcgtcgagcg ggccgccgcc gagggacgca agatcgaggt cgttctcgct gcggactggg
      301 aggagtccat cggcgtcaat gaccgcgagc agctcgcccg agccgaggcg gtgtatcgcc
      361 ggcgcatgct ccgtaccttg atggtggact gcggggtcac gatcatcgat ccggccacga
      421 catacatcga tgccggggtc gagatcgcgc cggatacgac gatcgagccg aatacccaca
      481 tccgtggtca cacgcgcatc ggatcgggct gcacgattgg gccgaacgcc atgctccgtg
      541 atgcacgcat cggcgagggc tgccgcgtgt tcgcatcgtg cttggaggat tgcgagctgg
      601 aagacggcgt cgatgtgggg ccgtacagcc atgtccgccc aggagcacac ctcgaggcgg
      661 gcgtgcatgt ggggaactac gtggaggtca aggcgagccg cattggacgc gattcgaaaa
      721 tcggccactt cagctatatc ggcgacgcgc tgcttggtgc caacgtcaac atcggggccg
      781 gcacggtcac ctgcaacttc gacggcgagc gcaagcacgc gacccacatc ggggatgacg
      841 cgttcatcgg tagcgacacc atgctcgtgg ctcccgttcg catcggccgg ggcgcgcgta
      901 ccggcgccgg cagcgtcgtg acccacgacg tcgcggaagg tgagacggtg gcaggcgtgc
      961 cagcgcgggg ccttcgccag aagcgcgcgg aaggtgcggt cgatggacgc tgaacgcctc
     1021 ctgagcatcg cgctgcttct cgtcatcggc gtcgtgcttg ggctcctctc gatggcggaa
     1081 gcaggggcca tcaccatcag ccgccagcgg gcacggcttc tcgccgagcg tggcgagcgg
     1141 cacggccgcg cactgcaccg ccaagccatc gagagggccg cgacctatgc gtcgttcacg
     1201 gtcatccgta gcgccctgct tatcggcgga acgattctgc tcgccgggct tctgatcgac
     1261 gcgctcgacg acgatctggc ggcgctcgcc ctggcgtact tcatcgccct cggcgtaacc
     1321 cttctgctgg agatcgcgcc cggatacatc gcggtgcagc agccggagcg atgggcgttg
     1381 cccttcaccc ccgtcgtgca agcaatacgg gttgtggtcg gaccgatcgc ttggctgttc
     1441 acgctgccgg cccaggtctg gctgcgcctc cgcggagtgg gcggcaacgg gggcgacgaa
     1501 gtgtcggagg cggaggagct tctgcgccag gtcgagatgg aacgaggcga ggagatcgag
     1561 cgcgaggagg cggagatgat ccgcggcatc ttcggcctcg aggatacgac ggcacgtgag
     1621 gtgatggtgc cacggatcga catggtcgcc gtcgaggctg gagagagcgt cgagacggcg
     1681 gtgcagacca tcgtcgaaaa gggcgtgagc cgcatcccgg tctacaagga caccatcgat
     1741 aacatcgtgg gcatcgtgta tgcaaaagat ctccttcgct acttggccgc cgcgtcgccg
     1801 cccgtcaccc tcactgacct ggcgcggcct gcgcacttca tccccgagac gaaacgcacg
     1861 gacgagctcc tgcgcgagct acggcagagc aaggttcaca tcgctatcgt cgtcgacgag
     1921 tacgggggca cggccgggct cgtgaccatc gaggacctgc tggaggagat cgtcggcgag
     1981 atcgaggacg agtacgatat cgccacgcag ctcatcgagg tcgtcagcga agcggaagcc
     2041 gttctggacg cccgcgtccc ggtcgacgag ctaaacgagc tgttcggaat cgaagtggac
     2101 cacgaggact acgatacggt gggtggcttc gtctaccacc accttgggac aataccgtcg
     2161 gttggcgacg agatcgaggt cgatggcatc cacgtgcgca ttctttccgt agaagatcgc
     2221 cggatcaaga aggtgcacat gaggcgggtc cagccgcccg atccgggggc aggcgccgag
     2281 cgccgcgagc gtgcctcctg aacgcggggg ccgcaccatc gtgagccagg agttgcccgc
     2341 tccagcccat cgagtctctc ccgtaggcct gcccgcggcc taccggctgc caccccgccc
     2401 tgccggcgaa ggagtcgatc agcaggtcgc cgatgcgcgg cgacagtcgt ctttcgtgct
     2461 gggggaagac gtccgattgt tcgagcgcgg catgaacctc cagatggacg tcctgcgcga
     2521 ctcgcaccct gtccggtacc ggacgcacgc cctcgcggca ctcgtcatgc agtggtcgcg
     2581 ggcgtatgcc tacgagcgcg acgccttcga gctggccctc cacggctcct atatctcggc
     2641 tctgccgttg ctgcgagcag cggcagaatg cctcgccgcg cagatgggac tccaccgcgg
     2701 cgatctgcct gaattcgagg cgtggctggg cggagtgggc ctgccgcaat cggcactgca
     2761 cgctgtccaa gtcccgctcg gccgctaccg atcgagcgct tccatcgccg atgacgccag
     2821 gctcggcccg atatatcgag cgacggccga tttggcgaac acccacttcg gatcgtcgct
     2881 cctccaagtc ggaccggaat cgaaccagca gaagctggcg ttggcgttcg gcgaccgcgc
     2941 cttccacctg ggctgggcgg aggtcgtgtt cggctgggcc cttgggctcg tgagccggca
     3001 actcacgttc gcctgcgagg cgcacgatgt gctgaacatc ggcgacgaac gccggtcgga
     3061 ggtggagtcg tatgcgagcg ccgtggcagg ggcactcgag cggcccgatc ggtgcgccat
     3121 catggagatc gaacacgaag gcgaacggcg cctgcttgtc cacaactttc gtcgggcgcc
     3181 cggcagtgcg ggcaggcgca ttctgctcta ggtcgcttag aatgggcaga aggggaacac
     3241 tgagagtcgg cgagcggcca cgggcagccg cagtacctca aggaggttcg agtccatggt
     3301 gacgaaggtt gggatcaacg gcttcggtcg tatcgggcgt caggtgttca aggcgatcct
     3361 cgagcggcat agcgatacgc tggaggttgt cgcagtgaac gacctgacgg atacggacac
     3421 caacgcgcac ctcttaaagt acgacagcaa ctacggccgc tttccgggca ccgtggaagc
     3481 gcgcgacggc gggctcgtcg tcaacggtaa ggatgtgcag gtgttcgcgg agcgggatcc
     3541 cgcgcaaatc ccatggcgca acgtgggcgc cgatgtcgtc gttgagtcga cagggcgatt
     3601 caccgatgcg acgaaggcgg ccggtcacac gcagggtggc gcgaagaaag tcatcatatc
     3661 ggcgccggcg aagaacgagg acctgaccgt tgtgttgggc gtcaacgagg gcatgtacga
     3721 tccggcgaag cacaccgtca tctccaacgc atcctgcacg acgaactgca tcgctcccgt
     3781 cgtcaaggtg ctgcacagct ccttcggcgt cgagaagggg ctgatgaaca ccatccactc
     3841 ctacacgaac gatcaggtca tcctggatac cgtgcacgat gatctgcgga gggcgcgagc
     3901 cgcggccatc aacatcattc ccacaacgac cggggcagcg aaggccgtca ccgtcgtcat
     3961 gcccgagctt cagggcaaga tccacgggct ggcgtttcgg gtgccgacgc ccaccgtgtc
     4021 cgtgtgcgac tttacggccg agttggggcg ccaggcgacg gtcgacgaag tgaacaaggc
     4081 cttcgaggac gcggcgcgcg aggcgctgaa aggcatcctc gcctacgaga cagaagagct
     4141 ggtgagcagc gacttcaagg agaatccgca cagcgccatc ttcgatgccc cgggcaccat
     4201 cgtcatcggt ggcaacatgg tgaaggtgct ggcttggtac gacaacgagt gggggtattc
     4261 gtgccgcgtg gccgacctgt gcgactacat cgcgaagcgg gggctttaga gccgctcaag
     4321 cgattgcggt agaatcaccc gggcgatgcg catccggccg gccgtatggc gtaaggacgg
     4381 tcatcagaac gaagggggca tgacgtgcag gggaaggtcg ctctcatcac gggcagcagt
     4441 cggggcatag gtcacttcat ggcggtcgag ttggcgaagg cgggagccga catcgtcgtt
     4501 gcggctcgga gcgagcagga gccggacccg cgcctgcccg gcactatcca tagcgtcgct
     4561 gcggagataa gcgcgctcgg gcgccgcgcg cttcccgtgc ggatggacct gacgaaggac
     4621 gaggaggtgg agcagggcgt tcaacaggcc attgcggaat tcgggcgcat cgatgtgctc
     4681 atcaacaacg ccggcatcat ggcccctgcg ccgctggtcg acctcccgat gaagcgttgg
     4741 gacctcgtga tgcgcgtcaa cgtgcgcggc gcggtcgtag cgacgaaggc cgtgctgccc
     4801 cacatgatgg agcgcaagtc gggcaccatc atattcatct cgtcggtggc ggcggagatg
     4861 tccggagcgg gcaacatctc gtacgctgtg tccaagctat cgctggagaa gattgcccag
     4921 gggctcgccg aggaggtgcg agatgacggc atcagcgtct tcggactggc gccgcgctcg
     4981 cttgtcataa cgccaggtaa cagcttcgtg cggggcgatg cgcgaccgga agggatcgcc
     5041 atcgagccac ctgagcagat ggggcaagcc gccatctacc tctgctcgga cgatgcgaag
     5101 cagtatagcg gccacacgtt ctacagcgat gccgcggcgg ctcggcgagg agagccggtg
     5161 cgataaccct
//
LOCUS       DATMBB010000297         2329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_448333, whole genome shotgun sequence.
ACCESSION   DATMBB010000297 DATMBB010000000
VERSION     DATMBB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448333"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VNL92_06360"
     CDS             complement(<1..1069)
                     /locus_tag="VNL92_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWO94370.1"
                     /translation="MDLVVDGDPVALALRTHEDVGGHIVPLDRERGVVRLVFDEGVAL
                     DLAPLRGGSLEADLALRDFTVDAIALPPPGAGGGAIDPYGGIDDIRRLRLRAVAKSIF
                     ADDGVRLLRAARIAADFGLAPDGALVAAARTDAAHIVDASAERRRDELVRLLATDHAA
                     LGLRMLDQFAVLDSLAPELAATRGVAQPKEHYYDVFDHCIETVAALDVLLSSLVPAGR
                     AAEAMRSDFLDRLSLAMDVRRYIDSEPVEGHPIRALTKLGGLLHDIAKPQTKTVDATG
                     RMRFFGHADEGAERAAHFLRELRFSKRETEFVRLLVAEHLRPTQLRSGEGPPTDRAIN
                     RFFRDTGDASIAILVLSLADHM"
     gene            complement(1271..1768)
                     /gene="def"
                     /locus_tag="VNL92_06365"
     CDS             complement(1271..1768)
                     /gene="def"
                     /locus_tag="VNL92_06365"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWO94371.1"
                     /translation="MAILPIRIVPDPVLREKAKKVPQLDGSIQKLIDDMIDTMRDAPG
                     VGLAAPQVGVSLRLVVIETPEMDTPLALINPEVVKASGERLVEEGCLSIPGYKAEITR
                     HVQVYVKARSRDGREIRVKASDDLLAQALEHEIDHINGILYTDYLDSPDKLKRLEPAG
                     QPDLE"
     gene            complement(1835..>2329)
                     /locus_tag="VNL92_06370"
     CDS             complement(1835..>2329)
                     /locus_tag="VNL92_06370"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWO94372.1"
                     /translation="DIGLHVPDYRSGERTYQLLSQVAGRAGRGPLGGRVIIQSYLPDH
                     YAIDAAARHDYEAMYAAEIASRRALGYPPFGRLAQLTYTGRGEQRSLAAAGRVVRQLS
                     EEIRARGVPGAWVRGPAPAYVPRWRGQWRWHVVLRAPDPPDVLRSFALGEGWEVDIDP
                     VSLA"
BASE COUNT          411 a          781 c          778 g          359 t
ORIGIN      
        1 ccatgtgatc ggcgaggctg aggacgagaa tcgcgatcga ggcgtcgccg gtatcgcgga
       61 agaaccggtt gatggcccga tccgtgggag gaccttcgcc cgagcgcaat tgcgtaggcc
      121 ggaggtgctc cgcaacaaga agacgaacga actcggtctc ccgcttgctg aatcgcagct
      181 cccgcaggaa atgggccgcg cgctcggcgc cttcgtccgc gtgcccgaag aaccgcatgc
      241 gccccgtagc atcgaccgtc ttggtctgcg gtttcgcgat gtcgtggagc aggccgccga
      301 gcttggtgag cgcacggatt gggtgtccct cgaccggttc gctgtcgatg taccggcgca
      361 cgtccatcgc cagggagagc cgatcgagga agtcggaacg catcgcctcc gcggcccgcc
      421 ccgccgggac cagggacgac aagagcacgt cgagcgcggc cacggtctcg atgcaatggt
      481 cgaacacgtc gtagtagtgc tccttcggtt gggcaacgcc acgcgtagcg gcgagctcgg
      541 gcgcgagcga atccagcacg gcgaactgat caagcattcg gaggccaagc gctgcgtggt
      601 ccgtcgccag cagccgcacc agctcgtcgc gacgccgctc cgccgatgcg tcgacgatgt
      661 gtgcagcgtc ggtgcgagcg gcagcaacga gcgccccatc cggcgcaagt ccgaagtcgg
      721 ccgcgatccg agcggctcgc agcaggcgaa cgccatcgtc ggcgaagatg cttttggcga
      781 cggctcgcag gcgcaaacgc cggatgtcgt cgatacctcc ataaggatcg atagcccctc
      841 cgccggcccc cggcggagga agggcgatcg catcgaccgt gaaatcccgc agggcgaggt
      901 cggcctcgag cgagccgccc cgcaatggtg cgagatcaag agcgacgccc tcgtcgaaga
      961 ccagccgtac gacgccgcgc tcccgatcga gcggcacgat gtgtccgccg acgtcctcgt
     1021 gcgttcgtag ggcaagagca accggatcgc cgtcgacgac gaggtccacg tcccgcggct
     1081 cgcggccgaa cgcgatgtct cgaacgaacc cgcccaccaa ccaacagggg gcagccgcgg
     1141 cgagggatcg gacagcttgg ccaaccggcc ccagcgtagc cagatggccg tgggcacgtt
     1201 cgacgagcga gtcggcaaga gtcgcagtca cgatcgcatg aggtcccgaa gccagcccgg
     1261 cgatggttcg ctactcgagg tcgggctgcc cggccggctc caggcgcttg agtttatcgg
     1321 ggctgtcgag gtaatcggtg tataggatgc cgttgatgtg atcgatttca tgctcgagcg
     1381 cctgggcgag gagatcgtcg ctcgccttca cgcggatctc tcggccatcg cgcgagcgag
     1441 ccttgacgta gacctgcaca tgccgcgtga tctccgcctt gtatccggga atggagagac
     1501 agccctcctc cacgaggcgc tcgcccgaag ccttcacgac ttcaggatta atgagggcaa
     1561 gcggcgtgtc catctccggc gtctcgatga caacgaggcg cagcgagacg cccacttgcg
     1621 gcgcagcaag tccgacgccg ggcgcgtcgc gcatcgtgtc gatcatgtca tcgatgagct
     1681 tttggatcga cccgtcgagt tgcggcacct tctttgcctt ctcgcgcaac acgggatctg
     1741 gaacaatccg gatgggcaga atcgccacgg ctcaccctcc gccgtcccgg ctatcgtcgc
     1801 caagacgatc atcattctat gctcgttgcc gaacttacgc taggcttacg ggatcgatat
     1861 cgacttccca gccctccccg agggcgaagg agcgcaggac gtccggcggg tcaggcgccc
     1921 ggagcacgac atgccagcgc cactgccccc tccaccgcgg gacgtaggcc ggcgcggggc
     1981 cgcgaaccca agcgccggga acgccacgcg cgcggatctc ctccgagagc tgtcgcacca
     2041 cgcgtccggc cgcggccagc gagcgctgtt cgccccgccc tgtgtacgtg agctgtgcga
     2101 gccggccgaa cggcgggtac ccgagcgccc ttcgcgatgc aatctcggca gcgtacatcg
     2161 cctcgtaatc gtgacgggcc gcggcatcga tggcgtagtg atcggggagg tacgactgga
     2221 tgatgacgcg gcctccgagc ggccctcggc ctgcccgccc ggcgacctgc gagaggagct
     2281 ggtaggtccg ctccccgctg cgataatcgg gcacatgcag gccgatatc
//
LOCUS       DATMBB010000298         6807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_413997, whole genome shotgun sequence.
ACCESSION   DATMBB010000298 DATMBB010000000
VERSION     DATMBB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6807
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413997"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VNL92_06375"
     CDS             complement(<1..970)
                     /locus_tag="VNL92_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWO94373.1"
                     /translation="MVVSAKSTVTIGQLLERCRTYLPEDRVELIERAYEFAERAHSGQ
                     LRVSGEPYINHPIGAAMTVAELQLDASSIAAALLHDVVEDCGVVIDEVQANFGDEVAK
                     LVEGVTKLSRMQWVAPEGRIGDEEIQAENLRKMFLAMAEDNRVVLVKLADRLHNMRTL
                     DALPPEKRLRIARETMDVYAPLASRLGIWQMKWELEDLSFRHLDSKQYKEISQLVAAR
                     RTERERHVQQVETTLRDELEAHGIHAEVRGRAKHIYSIYKKMQRYTRQGKTFDQIYDL
                     LALRVIVDEVSQCYQALGVVHNLWKPMPGQFDDYIAQPKESMYQSLH"
     gene            1066..2457
                     /gene="hisS"
                     /locus_tag="VNL92_06380"
     CDS             1066..2457
                     /gene="hisS"
                     /locus_tag="VNL92_06380"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWO94374.1"
                     /translation="MFQSPRGTFDILPADQPYWRHIRDHVEAWCARFGYERIDTPIFE
                     EAALFTHATGQGTDIVEKEMYTFEDRSGDLLALRPEGTPNTLRAYVQHGMASLPQPVR
                     LYYWAPSFRYERPQAGRYRQFWQFGCEAIGEADAAIDAEQIELCVRLYDSLGLRNIQL
                     QLNSIGDATCRPAYIDSLRAYYAPRLDVVCPDCRQRYERNALRLLDCKNPACQPIIAE
                     APVITHHLCADCSAHFASVRAYLDEIGIAYVIAPRLVRGLDYYTRTVFEFVPEGGGQQ
                     STVGAGGRYDGLMETLGGRPTPGTGFALGIDRLVLNLKEAEAYVPPLPRAEVYVAYQV
                     EEARGAATRVASALRSAGIGTIAGAGRSLKSQLRQADALGMTYTVIIGRQELASGTAQ
                     VRRMADGGQYHVDLEDLPKALASLLDTGVEMHASYDQHRVDQDVGAAMTFGTPTQLQQ
                     DTGEGYVGPPPRD"
     gene            2432..3502
                     /gene="prfA"
                     /locus_tag="VNL92_06385"
     CDS             2432..3502
                     /gene="prfA"
                     /locus_tag="VNL92_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWO94375.1"
                     /translation="MWDRLREIEARFEELTKQMGDPDIATDHVRLQALAKERAAIEEI
                     VELHRRYREVQAQLQDARSLEGETMDRELLTLVRDEVQTLEAEEERLEAALKRALLPK
                     DPRDERDVIVEIRAAAGGEEAALFAADLYRMYNRYADRRGWKTEIVSATESDKRGFRE
                     VIFEVHGTGAYSRLKYESGVHRVQRVPETEAQGRIHTSTATVAVLPEAEDVDVAIDEN
                     DLRIDVFHSGGAGGQNVNKVATAIRITHKPTGIVVVCQDERSQLKNRMKAMAVLRARL
                     YDLEQRKQMSEMAETRRTQVGTGDRSEKIRTYNVPQDRVTDHRIGLTLHNLPAILDGD
                     LDALIDAVATSEEARLLQEQVA"
     gene            3499..4362
                     /gene="prmC"
                     /locus_tag="VNL92_06390"
     CDS             3499..4362
                     /gene="prmC"
                     /locus_tag="VNL92_06390"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWO94376.1"
                     /translation="MNLWEARHTAAKRLERAGIEDPDLEAEVLLRHILHRDRAAFFAH
                     EDEDLDPPTADAFTALLNRRLAHEPLAYITGSREFYGLDFHVTPAALIPRPETEMLVE
                     ATLARLSAMRTEAGPAHAIDVGSGCGAVAIAVAANRPGARVLATDVSAPALLLTQENA
                     SRHGVAARIDLLETDLLRGVRGQIAVIAANPPYVRSDEWERLPAEIREHEPRVALDGG
                     PDGLEVIRRLLSQAQPLIEAGAALYAEIGDGQGKAALAAARPLFRAFADIGIRRDLAG
                     RERMLCVEPRG"
     gene            complement(4391..5530)
                     /locus_tag="VNL92_06395"
     CDS             complement(4391..5530)
                     /locus_tag="VNL92_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015406932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HWO94377.1"
                     /translation="MSAPQYKELRHAYGFDDVAIVPGDVTVNPDLVDVSTELGDTRFD
                     IPIIASAMDAVVDVDFAVRYSKMGGLAVLNLEGLQTRYDDPDAVLAELGEAPKERATE
                     LLQKAYNAPIRPELVGERVRAIKERQGIAAVACAPAWTKRLAPIAAEAGGDFFFVQST
                     VTTARHISRSERGLRLDELCRTLPMPVLVGNTVGYHATRELMEMGVAAVLVGVGPGAA
                     CTSREVLGIGVPQVTATIDCAAARDHHYSETGRYVPIITDGGMRSGGDICKAFAAGAD
                     AVMIGSPFAAIEGAPGRGHHWGMATPNADLPRGTRVEVGRRTTLERLLNGPTSRTDGT
                     ENLVGALRTAMGMCGARNIREFHTAEMVIAPAIKTEGKLYQMAQM"
     gene            5788..6603
                     /gene="rsmI"
                     /locus_tag="VNL92_06400"
     CDS             5788..6603
                     /gene="rsmI"
                     /locus_tag="VNL92_06400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWO94378.1"
                     /translation="MPTLYLVATPIGNLDDITLRALQTLRQVALIACEDTRTTRHLLA
                     HFDIRQPTLAYNEHNHTARLPRILATLEGGDVAFVSEAGMPAISDPGEMLVRAAIDAG
                     HRVIALPGPSAVVTAIAVSGLPPRPFTFLGFLPRSAKERRKLLASMAPRPDTLVAFEA
                     PHRLREALEDAMAMLGERRIAVCRELTKLHEEVFRGTLREALAYFQEPRGEFTLVIEG
                     APRGSGRIDEHQVLEELRALRDQGVSAKQAIAQLALTQGLSRRELYRLWLQLA"
BASE COUNT         1196 a         2304 c         2164 g         1143 t
ORIGIN      
        1 aatgcaacga ttggtacatg ctctccttgg gttgagcgat gtagtcatcg aactgaccgg
       61 gcatcggttt ccacaggttg tgcaccacac ctagcgcttg ataacactgg gagacctcgt
      121 cgacgatgac ccggagcgcc agcagatcgt agatctgatc gaaggtcttc ccctgccggg
      181 tgtagcgctg catcttcttg tagatggagt agatgtgctt ggcacgaccg cgcacttcgg
      241 cgtggatgcc gtgcgcctcg agttcatcgc gcagcgtcgt ttccacctgc tgaacgtgac
      301 gctcgcgttc cgtgcgtcgg gcggcaacga gttgcgagat ctccttgtac tgcttcgaat
      361 cgaggtgccg gaacgagagg tcttcgagct cccacttcat ctgccagatc ccgaggcggc
      421 tggcgagggg agcgtaaacg tccattgtct cacgtgcgat gcggaggcgc ttttcgggag
      481 gcagtgcatc gagagtccgc atgttgtgaa ggcgatcggc gagctttaca agcacgacgc
      541 ggttgtcctc ggccatggca aggaacatct tgcgcaggtt ctcggcctgg atctcctcgt
      601 cgccaatgcg gccctccggg gccacccact gcatgcgcga tagcttcgtg acgccttcga
      661 cgagcttcgc cacctcatcg ccaaagttgg cctggacttc atcgatgacg acgccacaat
      721 cctcgacgac gtcgtgcagc agggccgccg cgatactcga tgcatcgagc tgcagctcag
      781 cgacagtcat tgctgcgccg atggggtgat tgatgtatgg ctcgcccgat acacggagtt
      841 ggccactatg cgcacgctcg gcgaactcgt aggcgcgctc gatgagctcg acgcggtcct
      901 cgggaaggta ggtacggcac cgctcgagca gctggccaat ggtgacggtg gacttggctg
      961 atacgaccat tatgtcattg catagtatac gccctccttc ggctcctcaa gccgggctcg
     1021 gcttcgctcg gtcgcttgct atactggtta cgaggacgat tcatcgtgtt ccagtcccca
     1081 cgcggcacct ttgatatcct gcccgccgac cagccctact ggcggcacat tcgcgaccac
     1141 gtcgaagcat ggtgcgcccg ctttgggtac gagcgtatcg acacgccgat cttcgaggag
     1201 gccgcgctct tcacgcacgc caccggccag ggtaccgaca tcgtcgagaa agagatgtac
     1261 acgttcgagg atcgcagcgg cgatctcctg gctctacgcc cggaggggac gccgaatacg
     1321 ctccgggcct acgtgcagca cggcatggcg agcttgcccc agcccgtgcg actgtactac
     1381 tgggcgccgt cctttcgtta tgagcgcccg caggcgggcc gctaccgtca gttttggcag
     1441 ttcggctgcg aggctatcgg cgaggccgac gctgccatcg atgccgagca gatcgagctt
     1501 tgcgtccgcc tctacgactc gctgggtctg cgcaatatcc aattgcagct caacagcatc
     1561 ggcgatgcca cctgccggcc ggcgtacatc gattccctgc gtgcgtacta cgccccacgc
     1621 ctcgatgtgg tctgtcccga ttgccggcag cggtacgagc ggaacgccct gcgcctgctc
     1681 gactgcaaga acccggcgtg tcagccgatc atcgccgagg cgccggtcat tacgcatcac
     1741 ctctgcgccg attgctccgc tcacttcgca agcgtgcggg cgtacctcga cgagatcggc
     1801 atcgcctacg tcatcgcccc ccggctggtg cggggactcg actactacac gcgcacggtc
     1861 ttcgagttcg ttccagaggg aggcgggcaa cagagcaccg tcggcgccgg cggacgatac
     1921 gacggcctga tggagacgct ggggggccgg ccaacaccgg gaacgggctt cgccctcggc
     1981 atcgatcgac tcgtgctgaa cctgaaggaa gccgaggcct acgtcccgcc cctcccccga
     2041 gccgaggtgt acgtcgcata tcaggtcgag gaagctcgcg gggcagccac gcgcgttgcg
     2101 tctgccctcc gttcggccgg catcggaacg atcgccgggg cgggacgctc gctcaagtcg
     2161 cagcttcgcc aagccgatgc cctcggcatg acctacaccg tcatcatcgg tcgccaggag
     2221 ctcgcgtcag gcacggcgca agtacgccga atggccgatg gcggccagta ccacgtcgac
     2281 ctggaggacc tgccgaaggc gctcgcgtcc ctgctcgata ccggtgtgga gatgcacgcg
     2341 tcgtacgatc agcatcgggt ggaccaggac gtgggagcag caatgacgtt cggcactcca
     2401 acgcaactac agcaagatac cggagagggc tatgtgggac cgcctccgcg agattgaggc
     2461 tcgcttcgaa gagctgacca agcagatggg cgatccggac atagcgacgg accacgtgcg
     2521 gctgcaggcc ctagcgaagg aacgtgcagc catcgaggag atcgtggagc tgcatcgccg
     2581 ctaccgcgaa gtccaagctc agctgcagga tgcacgctcc ctcgaaggcg agacgatgga
     2641 ccgcgagctc ctcaccctgg tccgtgacga ggtgcagaca ctcgaggcgg aggaggagcg
     2701 gctcgaagca gccctgaagc gcgccctgct tcccaaggac ccgcgcgacg agcgcgacgt
     2761 catcgtcgag atccgagcgg ccgctggcgg cgaagaggca gcgctctttg cggccgacct
     2821 ctatcgcatg tacaaccgct atgccgaccg ccgcggctgg aagaccgaga tcgtgtccgc
     2881 caccgagagc gacaagcgcg gcttccgcga ggtcatcttc gaagtgcacg gaaccggcgc
     2941 ctactcgcgg ctcaagtacg aatcgggcgt ccaccgcgtc caacgcgtgc cggagacgga
     3001 ggcacagggc cggatccaca cctcgacggc tacggtcgcc gtccttcccg aggccgagga
     3061 cgttgatgtc gcgatagacg agaacgacct ccgcatcgat gtcttccact ccggcggcgc
     3121 cggcggccaa aacgtcaaca aggtcgcgac cgccatacgc atcacgcata agcccacggg
     3181 catcgttgtt gtctgtcagg acgagcgttc gcagttgaag aatcgcatga aggcgatggc
     3241 ggtccttcgc gctcgcctgt acgacctgga gcagcgaaag cagatgagcg aaatggcaga
     3301 gacgcgccgc acgcaggtcg gcacaggcga ccgctcggag aagattcgaa cctacaacgt
     3361 gccgcaggac cgggtcaccg atcaccgcat cggactgacc ctgcacaacc tccctgcgat
     3421 cctcgacggc gatctcgatg ccctcatcga cgctgtcgcg acgagcgaag aggcgaggct
     3481 gctgcaggaa caggtggcgt gaacctgtgg gaggcccgcc acaccgccgc caagcggctg
     3541 gagcgggccg gcatcgaaga cccggacctc gaggccgaag ttctgttgcg ccacattctc
     3601 catcgagatc gcgctgcatt cttcgcgcac gaggatgagg atctcgaccc gcctactgcc
     3661 gatgcgttca cggctctcct caatcgccgc ctcgcccacg agccgctggc ctacatcacg
     3721 ggctcgcgcg agttctacgg cctcgacttc cacgtcaccc cggctgcgct gatccctcgt
     3781 cccgagacgg aaatgctcgt cgaggcaacg ctcgcgcggc tctccgcgat gcgtacggaa
     3841 gcaggccctg cgcacgccat cgatgtcggg agcgggtgcg gcgccgtggc catcgccgtc
     3901 gccgcgaacc gccccggcgc ccgcgtcctc gcaaccgatg tctcggcgcc cgccctgctg
     3961 ctgacgcagg agaacgcgag caggcacggc gtcgccgcgc gaatcgacct cctggaaacg
     4021 gacttgctgc gtggcgtccg aggccagatc gccgtcatcg ccgccaaccc gccttacgtg
     4081 cggagcgacg agtgggagcg gctgcccgcc gagatccgcg agcacgagcc acgtgtcgcg
     4141 ctcgacggcg gaccggatgg cttggaggtc atccgccggc tgttgtcgca ggcgcagccg
     4201 ttgatcgagg ccggcgccgc actgtacgcg gagatcggtg acgggcaggg caaggccgct
     4261 ctcgcggcgg cacggccgtt gttccgcgca ttcgcggaca tcggcatccg ccgagacctg
     4321 gccggccggg agcgaatgct atgcgtagag cccagagggt agcgaagaac ctcgaagcgc
     4381 cgaggggaag ctacatctgc gccatctggt agagcttgcc ttcggtcttg atggccggag
     4441 cgatgaccat ctccgccgtg tggaactcgc ggatgttgcg cgctccgcac atgcccatcg
     4501 ccgttcgcag cgccccgacg agattctccg tgccatcggt gcgggacgta ggaccgttga
     4561 gcagcctctc gagggtggtg cggcggccca cttccactcg ggtgccgcgc ggaaggtcag
     4621 cgttgggcgt ggccataccc cagtgatggc ctcggccagg cgcgccttcg atggcggcga
     4681 acggagagcc gatcatcacg gcgtcggctc ccgctgcgaa cgccttgcaa atgtcgccgc
     4741 ccgagcgcat ccctccgtcc gtgatgatgg ggacgtagcg cccggtttcg ctgtaatggt
     4801 ggtcgcgcgc cgccgcgcaa tcgatggtgg ccgtcacctg gggaacgccg atgccgagga
     4861 cctcgcgact cgtgcaagct gcgccgggcc cgacgcccac gagcaccgcg gccacgccca
     4921 tctccatgag ctcccgggtc gcgtggtagc cgaccgtgtt gccgacgagg acgggcattg
     4981 gcagcgtgcg gcacagctca tcgagccgga ggccccgctc gctccgcgaa atgtggcgag
     5041 ccgtcgtgac ggtgctctga acgaagaaga aatcgccccc agcttccgca gcgatggggg
     5101 cgaggcgctt cgtccaggcg ggcgcgcacg ctacggctgc gatgccctgc cgctccttga
     5161 tggcgcgaac acgctccccc acgagctcgg ggcggatcgg tgcgttgtag gccttctgga
     5221 ggagctccgt ggcgcgctcc ttcggcgctt cgccaagctc cgcgagcacc gcgtccgggt
     5281 cgtcgtagcg ggtctgcaga ccttcgaggt tgagtacggc aaggccgccc atctttgagt
     5341 agcgcaccgc gaaatcgaca tcgacgacgg catccatggc cgaggcgatg atcgggatat
     5401 cgaaccgggt atcgcccagt tcggtggaga cgtcgacgag atcggggttg accgtcacat
     5461 cgccaggtac gatggcaaca tcgtcgaacc cgtaggcgtg gcggagttcc ttgtattgcg
     5521 gtgcggacat gggtacctcc ttcttcggtc cggaggccaa gccttccggc cagtctccca
     5581 cgatgcacaa gtgcccttcc gtggaagggc acttttctcg ctagttcggg cgcattccgc
     5641 cccgcttgca cgacatcggt atgaccctca acgccgtccc tccgcgagcg gatatgttcc
     5701 tcaaactata cgacagagtc cgaaggccgt caacgcgcgc ctcgcccttc caacaatcgc
     5761 gccgccccgc acggatataa tcgcagcgtg cctacgctct atctcgtcgc cacgcccatc
     5821 ggcaacctcg acgacatcac cctccgggcc ctgcaaaccc tgcgccaggt cgcgctcatc
     5881 gcctgcgagg atacgcgaac cacccggcat ctcctggcgc acttcgatat ccggcagccc
     5941 acgctcgcct acaacgagca caatcacacc gcccgcctcc ctcgcatcct cgccacgctc
     6001 gaagggggcg atgtcgcgtt cgtctcggag gccggcatgc cggccatcag cgaccccggg
     6061 gagatgctgg tgcgcgccgc catcgacgcc ggtcatcgcg tcattgccct gccgggaccg
     6121 tccgccgtcg tgaccgccat cgccgtctcc gggctcccac ctcggccttt cacgttcctc
     6181 ggcttcctcc ccaggagcgc gaaggagagg cgaaagctgc tcgcctcgat ggcgccgcgc
     6241 cccgacaccc tcgtcgcctt cgaagctccg caccggctgc gggaagcact cgaagatgcg
     6301 atggcgatgc tcggcgagcg tcggatcgcg gtctgccgcg agctgaccaa gctccacgag
     6361 gaggtgttcc gaggcacgtt gcgcgaagcc ctcgcgtact ttcaggagcc ccgcggcgag
     6421 ttcacgctcg ttatcgaagg ggcgccacgc ggatcgggcc ggatcgatga gcaccaggtg
     6481 ctcgaggagc tacgcgcgct tcgcgaccag ggcgtttcgg ccaagcaagc tatcgctcag
     6541 ctcgcgttga cccaggggct ctcccgtcgc gaactctacc gtctctggct tcagctcgcg
     6601 taaccgcggc gtagcatcga cttcatcaat ccgaaatgcg aacgagacgg cagcgtaacg
     6661 caccgtacct atgctacaga caacgggatc gggtcagagg acccgacgac ctagccctca
     6721 ggaggagaag taccatggcc atcatcgccg aaccacgagc gctctccccg ctcgaacgca
     6781 tcgccgggcg gcggcgaacc cgccgta
//
LOCUS       DATMBB010000299         3431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_97721, whole genome shotgun sequence.
ACCESSION   DATMBB010000299 DATMBB010000000
VERSION     DATMBB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97721"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VNL92_06405"
     CDS             complement(<1..248)
                     /locus_tag="VNL92_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94379.1"
                     /translation="MGCYEGVELQALDTMLSKIDPGLAGTQLHVDERVERHAPPTVMR
                     AVRWRAFRLIAGVIDGEPLVDGACDLDADQWIDLASRRL"
     gene            219..2897
                     /locus_tag="VNL92_06410"
     CDS             219..2897
                     /locus_tag="VNL92_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWO94380.1"
                     /translation="MELYALVTPHAGLATVTLALASQRSRGDSSAFDEEQHFFQVDLR
                     VAAVANGRFAPRPSRRAQTDEDSRTAALIYRDVKEYVVGHTCSARAILNGEQIAKLKI
                     EWVPAVIVPRVSDRGDQVFDALHQPTDDRRPLEAQWLAGASAPDLVAGLERLVAAYQQ
                     WITREAARIPSLPAELRAQAQKHIDRCRLGGQRMSDGIAAIRTDKSVRTAFQLAQSAM
                     ATQFGWSRNGASLKWRPFQLAFQLLVIPSLAERTHSDRDVMDLLWFPTGGGKTEAYLA
                     LTAFIIMLRRLRATKKDDGAGVAVLMRYTLRLLTVQQFQRAAAMIFACELLRRRSSSG
                     GAGLPDLGATPIGIGLWVGVAATPNTLKEALDRSPGDPSTPEQLTVCPCCGTRLNWKL
                     SPRESTVECPSEATTCELAQSGPRLPVWTIDEEVYRHMPSLLIGTVDKFAQIVRKPDT
                     AVIFGRGTSHAPPDLIIQDELHLISGPLGSMAGLYEVAIDELCRVDAIRPKVIGSTAT
                     IRRAEEQIRALFDRGTYQFPAPGLDAGNSGFAVTDLNTPGRLYVGLTTAGRSATYMLQ
                     ALVASLLQAATAPGASPQERDYYWTLVAYFNSLRELGRALVLMQDDVPVSIGQFAARR
                     GEQRRRIEAPAELTSRVRSYEIRDMLERLNKPASDSEAVDLLVASNMISVGMDIRRLG
                     LMVVNAQPKTLSEYIQATSRVGRGDAPGIVITMYNSMRARDRSHFETFETWHRCLYRD
                     VEATSVTPFASRAQDKALHAVLVALVRHLIPGMDRRPVLDDIRRTDAERIASTIEARV
                     ARVDPAERTIVSQKLQRLIDQWASRPDLQTYWDDYNRRTSLLMSAEQFAAKADVDADL
                     DAEGARRALWPTPNSMREVEPGAPFVLRHVLKTEGN"
     gene            2899..>3431
                     /locus_tag="VNL92_06415"
     CDS             2899..>3431
                     /locus_tag="VNL92_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94381.1"
                     /translation="MRNELGDVRRSQIITTHGPGSIVDFRAGGYGGAGVSVVAAGLEE
                     WDRWAPPPGLGHPQTVYEPRLQKQLGVDGFRLPPVAPQVAPGVYRNNAGKLVGVRFPR
                     WLQCPRCHLIRQSRYWNEDAGDPALYCADCSEKAGGRNRVHVVPVRFIVICERGHLDE
                     FPWDWWVKHDEKCPLPGR"
BASE COUNT          586 a         1153 c         1078 g          614 t
ORIGIN      
        1 aggcgtcggg aagcgaggtc gatccactga tccgcatcga ggtcgcatgc gccgtctaca
       61 agaggttcgc catcgatgac gccggcaatg aggcggaagg cgcgccaccg gaccgcgcgc
      121 atgaccgttg gcggcgcatg cctctcaacg cgctcgtcga catggagctg cgtcccggcg
      181 agaccaggat cgatcttgct gagcatggta tcgagggctt ggagctctac gccctcgtaa
      241 caccccatgc tggccttgcg accgtcacgc tggcccttgc gagtcagcgc tcacgcggcg
      301 actcgtcagc attcgacgag gagcagcact tctttcaggt cgacctccgg gttgccgccg
      361 ttgccaacgg tcgatttgcg ccgcgcccct cccgtcgcgc acagaccgac gaagacagtc
      421 gaacagctgc gctcatctat cgcgacgtga aggagtacgt cgtagggcac acgtgctctg
      481 cacgcgcgat tcttaatggc gagcaaatcg cgaagctcaa gatcgaatgg gtaccagccg
      541 tcatcgtccc gcgggtatct gaccgtggcg atcaggtctt cgacgctctc catcagccaa
      601 ccgacgatcg gcgtcccctg gaggcccagt ggctggctgg cgcatccgct ccggacctcg
      661 tcgcggggct cgagcgtctc gtagccgcat accagcagtg gattacgcgg gaagcggccc
      721 gcattccgag cttgcccgcc gagctccggg cgcaagcgca gaaacacatc gatcgctgcc
      781 ggctcggtgg gcagcggatg tccgacggga tcgcggcgat tcgcactgac aagagcgtgc
      841 gcacggcgtt tcagttggcc cagtccgcga tggcgacgca gttcggctgg tctcggaacg
      901 gcgcgtcact caagtggcgc ccgttccagc tcgcattcca gttgcttgtg atcccgtctc
      961 tcgctgagcg gactcacagt gaccgagacg tgatggacct tctgtggttc ccgacgggag
     1021 gtggcaagac cgaggcgtac ctggcgctca cggccttcat catcatgctg cgaaggcttc
     1081 gcgccaccaa gaaggacgac ggcgccggtg tcgccgtcct gatgcggtac acgcttcgcc
     1141 tcctgacggt gcagcagttc cagcgcgctg ccgccatgat cttcgcgtgc gagctccttc
     1201 ggcgacgtag ctccagcggg ggcgcggggc ttcccgacct tggcgcaacg cctattggca
     1261 tcggcctttg ggtaggtgtc gccgccactc caaacacctt gaaggaggcg cttgatcgat
     1321 caccaggtga tcccagcacg cccgagcagc tcacagtgtg cccgtgctgc ggtacaaggc
     1381 tcaactggaa gctctcgcct agggagtcca ctgtcgagtg cccctccgaa gcaaccacgt
     1441 gcgagctggc gcaaagcgga ccacggctgc cagtgtggac gatcgacgaa gaggtgtatc
     1501 gccacatgcc gtcgctgctt atcggcacgg tggacaagtt cgcgcagatc gtccggaagc
     1561 ccgacacggc tgtgatcttt gggcgcggca cttcgcacgc tcctcctgac ctcatcattc
     1621 aggacgagct gcatcttatc tccggccccc tcggatcgat ggccggtctc tacgaggtcg
     1681 ccatcgatga gctgtgtcga gtcgatgcca ttcgaccgaa ggtcatcgga tcgacagcca
     1741 caatccgtcg agccgaggaa cagattcgag cgctgttcga tcgcggcacc tatcagtttc
     1801 cggcgcctgg cctcgacgcc ggcaactccg ggttcgctgt taccgatctc aacacccccg
     1861 gacgcctcta cgtggggctc accacagccg gccggtccgc gacgtacatg ttgcaggcgc
     1921 ttgtcgcgtc gctcctccag gccgccacag cgccaggcgc ttcgccgcaa gagcgcgact
     1981 actactggac gctcgtggcc tacttcaact ctctgcgcga gttggggcgc gcgctggtcc
     2041 tgatgcagga cgacgttccc gtttccatcg ggcagttcgc cgcgcgacgc ggtgagcaac
     2101 ggcgtcgcat cgaggctcct gccgagctca cgagtcgcgt ccgttcttac gagatacgcg
     2161 acatgctgga gcggctgaac aagccggcca gcgattcaga ggctgtcgac ctcctcgtcg
     2221 cctcaaacat gatctccgtc ggcatggaca ttcgccggct cgggctcatg gtcgtgaacg
     2281 ctcaaccgaa gaccctctcc gagtacatcc aggcgactag ccgcgttggc cggggcgacg
     2341 ctcccggcat cgttatcacg atgtacaaca gcatgcgcgc tcgggaccgc tctcacttcg
     2401 agaccttcga gacgtggcac cgctgcctgt accgcgacgt cgaggcaacg agcgtcacgc
     2461 cctttgcgtc gcgagcgcag gacaaggctc tccacgctgt gctcgtggcg ttggtgcgcc
     2521 acctcatccc gggcatggat cgccgtccgg ttctcgacga cattcgtcga acagacgccg
     2581 agcgcatcgc gtcgaccatc gaggcgcggg tggcgagggt cgatccagcg gagcgcacca
     2641 tcgtctctca gaagctgcag cgcctcatcg accagtgggc gagccggcct gacctccaaa
     2701 cgtactggga cgactacaac cggcgcacca gcctcctcat gtcggctgag cagttcgccg
     2761 ccaaggcgga tgtggacgcc gatctcgacg ctgagggcgc gcgacgggcc ctatggccga
     2821 cccccaactc gatgcgcgag gtcgagcccg gggcaccttt cgttctgcgc cacgtcctca
     2881 agacggaggg aaactgagat gcgcaacgag ctgggagacg tccgtcgtag ccagatcatc
     2941 acgacccacg gaccgggatc catcgtcgac ttccgcgctg gaggctacgg cggagctgga
     3001 gtctccgtcg tcgccgccgg gctcgaggag tgggatcgtt gggcacctcc tccggggttg
     3061 ggccatcccc agacggtcta cgagccgcgg ctccagaagc agcttggtgt cgatggcttc
     3121 cgacttcctc cggtcgcgcc ccaggtagcg cccggcgtct atcgcaacaa cgccggcaag
     3181 ctcgtcggtg ttcgcttccc gcgatggctc cagtgcccac gctgccatct cattcgccaa
     3241 tcgcggtact ggaacgagga cgccggcgat ccggcgctct actgtgcgga ctgctcggag
     3301 aaggcgggcg gccgcaaccg cgtccatgtc gtgccggtac gattcatcgt catctgcgag
     3361 cgtggccacc tcgatgagtt tccctgggac tggtgggtaa agcacgacga gaagtgtccg
     3421 cttcccggcc g
//
LOCUS       DATMBB010000300         4068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_76552, whole genome shotgun sequence.
ACCESSION   DATMBB010000300 DATMBB010000000
VERSION     DATMBB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76552"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..666)
                     /gene="lexA"
                     /locus_tag="VNL92_06420"
     CDS             complement(28..666)
                     /gene="lexA"
                     /locus_tag="VNL92_06420"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWO94382.1"
                     /translation="MIKRAPDSRLRPADRKALAYIRNRIVHAGAAPSVRDLAAELGFS
                     SPRSAADVIDRLIEQGYLKRRDDRSLQLLRMPEDGRDRDSTVEVPLVGTAPCGSPLLA
                     QENVQGMIPVSTRLARPGQRYFLLRAIGDSMTEVGIEDGSLVLVRQQPTAEVGDVVVA
                     LVDDEATIKELRRSADAVALTPRSKNKAHKPIILRRDFQVQGVVVATLPDLD"
     gene            complement(738..1652)
                     /locus_tag="VNL92_06425"
     CDS             complement(738..1652)
                     /locus_tag="VNL92_06425"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HWO94383.1"
                     /translation="MTTRSARARRQRFSRSTSVRVRLTPDDVAIIRHIARHRFLRSTH
                     IVQLFPKRPAKKLIERLGLLYHAGFLDRPRAQIDYYLRHGSARMVYGLGSLAHVALPE
                     LGLIDWQKKNRTASQGFIKHELMLADIMVAIEVAAQSRRDLEYVPAEELRPTPGEHWR
                     LATTITIRGNSTELAIIPDAVFALRFNPSGRMAYFFVEADRRNMPVHRSDLNQSSFRK
                     KALVYHHAAMQRTIVDRFGFTNFRVLTVTSTPARIASMQREVKSITRSPSGRFLFASR
                     DLFAVRDPLSFEWSTLPGPSRLDTPPRA"
     gene            complement(1639..2907)
                     /locus_tag="VNL92_06430"
     CDS             complement(1639..2907)
                     /locus_tag="VNL92_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWO94384.1"
                     /translation="MVGTTTTGLSLDAPVTLSLDDRRRHLHIIGKTGTGKSTLLYALM
                     LQDLAAGRGFALLDPHGDLAQSVIDSVPPARTNDVLYLNPADLGHPVGFNLLENVRPD
                     ARALVTAHVVAAFKHVWPDSWGPRLEQILRNSIALLLDTPGSTLLGISRLLVDDAYRA
                     LLLKSCQNPMVRLFWTQQLAEWGDSFAAEAISPVQNKVDALLSPPVLRNIIGQPKSTL
                     DLAAIMNGRRVLIANLSKAALGEGPAHLLGAFLATAFAQQAEARAAIPERDRVDFYLY
                     ADEFQNFATDSFATILSEARKWRLSLTLAHQFLDQLSPGLRGAVLGNVGSLIAFRSGA
                     KDARTIAEEIGIENPEAVSSLPNFVAWGMLMRDGNPAGTQRIDMLEPHPLAYGRAGAI
                     IARARARHARNRADVEERIARFLHPHHDDA"
     gene            3762..>4068
                     /locus_tag="VNL92_06435"
     CDS             3762..>4068
                     /locus_tag="VNL92_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94385.1"
                     /translation="MSDTDIVPFAAAVAGDVLSAFNLPGGNSLTSVATAWLQRRRREA
                     AQVLINEISKGHHGEISFEQHDIDPLIEIIYRFSKAVADGAAVDNLRFLAQVIAGLKK
                     "
BASE COUNT          746 a         1177 c         1448 g          697 t
ORIGIN      
        1 gcgggattgg caggcttgct catggtgcta gtccaagtcc gggagggtag ccacgaccac
       61 gccctgcact tggaaatcgc gacggaggat gatcggctta tgggccttgt tcttagatcg
      121 gggagtgagc gcgacggcgt cggccgaccg ccgaagctcc ttgatcgtcg cctcgtcatc
      181 gaccagcgcg acgacgacat cgccgacttc cgccgtaggc tgttgacgga cgagcaccag
      241 gctgccgtcc tcgatcccaa cctctgtcat cgagtcgccg atggcgcgca gaaggaaata
      301 gcgctggccc ggccgcgcga gcctcgttga gaccgggatc atgccttgca cgttctcctg
      361 cgcaagcaac ggcgagccgc atggcgcggt gccaacgagg ggcacttcca cggtcgagtc
      421 gcgatcgcgg ccatcttccg gcatgcgcag cagctgaagc gagcggtcgt cgcgccgctt
      481 caggtagccc tgctcgataa gccgatcgat gacgtcggct gctgagcgtg gagaggagaa
      541 gccgagctcg gccgcgagat cgcgcacgga gggcgcggcg cccgcgtgca cgatgcggtt
      601 gcggatgtag gccagggctt tgcgatcagc cggtcggagc cgcgaatcgg gtgctctctt
      661 aatcatcacc acacagtatg tatgccggtg tatggcatgt caacagaatg accgtcttgt
      721 gcacaaccca tcaggcctca cgcccgcggg ggcgtatcga gacgagacgg acccggaagg
      781 gtagaccact cgaacgagag gggatcgcgc acggcaaaga ggtctcggct tgcgaacagg
      841 aatctgcccg agggggaacg cgtgatcgac ttcacctcgc gttgcatcga tgcaatgcgt
      901 gctggcgtcg acgtaacggt gaggacgcga aaattggtga agccgaaccg gtcgacgatc
      961 gtccgctgca tcgctgcgtg atggtagacc agggccttct tccggaatga cgattggttg
     1021 agatccgagc ggtgcacagg catattgcgc cgatcggcct cgacgaagaa gtaggccatg
     1081 cgcccgctcg gattgaagcg gagcgcaaag accgcgtcgg gtatgatcgc gagctccgtg
     1141 ctgttgccgc ggatggtgat cgtcgtcgcg agccgccagt gctcgccggg ggtcgggcgc
     1201 agctcctcgg ccgggacgta ctcaaggtcg cggcgcgatt gcgcggcgac ctcgatcgcg
     1261 accatgatgt cggcgagcat cagctcgtgc tttataaacc cctggctcgc cgtgcggttc
     1321 ttcttctgcc agtcgatgag gccgagctcc ggcagtgcga cgtgcgcgag cgatcccaag
     1381 ccgtacacca tccgagcgct gccatggcgg agataatagt cgatctgcgc ccgcggccgg
     1441 tcgagaaagc cggcgtggta gagaaggccc agccgttcga tcagtttctt cgcgggccgc
     1501 ttcgggaaga gctgaacgat atgcgtcgag cgcaggaagc gatggcgtgc gatgtggcgg
     1561 atgatcgcga cgtcgtcggg cgtcagccgg acgcggacgg aggttgaccg ggagaaacgc
     1621 tgacgacgag cacgagcgct acgcgtcgtc atggtggggg tggaggaagc gcgcgatgcg
     1681 ctcctcgacg tctgcgcggt tgcgggcgtg cctggctcgc gcccgggcaa tgatcgcacc
     1741 ggcgcgccca tatgcgagcg ggtgcggctc gagcatgtcg atgcgctgcg tgccagcggg
     1801 attgccgtcg cgcatgagca tgccccaggc aacgaagttg gggagcgagg agaccgcctc
     1861 cgggttttcg atgccgatct cctcggcgat cgtgcgcgcg tccttcgcgc cgctgcggaa
     1921 ggcgatgagc gagccgacgt tgccgaggac agcgcctctg agcccgggcg agagctggtc
     1981 gaggaactgg tgggcgaggg tcagcgagag gcgccatttg cgggcctcgc tcagaatggt
     2041 cgcgaagctg tcggtcgcga agttctggaa ctcgtcggcg tagaggtaga agtcgacgcg
     2101 gtctcgctcg gggatggcgg cacgtgcctc ggcctgttgc gcgaacgcgg tcgcgaggaa
     2161 cgcgcccaag aggtgagcgg ggccttcgcc gagcgctgcc ttggagaggt tggcgatgag
     2221 gacgcggcgg ccgttcatga tcgcggcgag atcgagcgtc gacttcggct ggccgatgat
     2281 gttgcgcagg accgggggag agagcagcgc gtcgaccttg ttctgcacgg gagagatcgc
     2341 ctcggccgcg aagctgtcgc cccactcggc cagctgctgc gtccagaaca gccgcaccat
     2401 cgggttctgg cagctcttga ggagcagcgc gcgatacgcg tcatcgacta gtaggcgcga
     2461 gatgccgagc agcgttgatc cgggggtgtc gaggagtagc gcgatcgaat tgcgcaggat
     2521 ctgctcgagg cgcgggcccc agctgtcggg ccagacgtgc ttgaacgcgg cgacgacgtg
     2581 cgccgtcacg agcgctcgcg catccggccg cacgttctcg aggaggttga agccgaccgg
     2641 gtgcccgagg tcggccgggt tgaggtagag gacgtcgttc gtgcgcgcgg gtgggacgct
     2701 gtcgatgacg ctttgcgcca ggtcgccgtg aggatcgagg agcgcaaagc cgcgaccggc
     2761 cgcgaggtct tgcagcatga gcgcgtagag gagcgtggac ttgccggtgc cggtcttgcc
     2821 gatgatgtgc agatggcgcc ggcgatcatc aagcgagagg gtgacgggcg catcgagcga
     2881 aaggcccgtc gtggtggtgc cgaccaaaat ggaatcatcc gcgtccatag gtcggcaccc
     2941 gggccggtga tcttccagcg ctctgccgga agggacgagg catctcgtgc gtcaagagcc
     3001 tgccggttgc ggatgccggc cggtgagcgc ggccgtgttg gctccattgt agggccggga
     3061 aaaacgtgcc ctgtggaaaa cgggctcgtc ctcgtccgca cctctcggcc gaacccgttt
     3121 gctggcgtcg cgcatagctg aaggattgcc ggcatcggtt cacgccgagg cacgcgatcg
     3181 cacccgctct gtcggcctga atgctgtcga aaccgatgca gctcgagaat gctggtgcgc
     3241 cttgccgatg accgcagcga agcggccgac aaatatggct gcggcatttg tcggcgagga
     3301 aaaatcgcaa gaagcggagc gctcgtcaaa gctcgcgcgc tgtcgtttcc gatgaggaga
     3361 aggcgatgtg aaagccatat ttgtcgggcg ctaagcccga cagagcggga cttgagaaaa
     3421 acagtcgtac tgagccaaat aatcgacatc gagggtgggg atatgatggg ggatatcatg
     3481 tatccccacc tgtctccccc cttttctctc acggacattg ccatgtccta taccgccccg
     3541 gccccgccgt agcctgcgca ggcgggttga gcgcgcgaag cgcgccctgt cggggctcgg
     3601 gatggcggcc aggccctcta ggatggcctg tatggcacga tatggcgaag gtttgcgcgc
     3661 ctgaggggtc gccctgcaaa aaggcacgtc cagggcagag tgcagggtat gctgcaaggt
     3721 aaggcgtcga tcagtatcca gcgacgagga ggaaaaggac ggtgagcgac acagacatag
     3781 tcccgttcgc agcggcagta gctggcgatg tgctgtccgc cttcaactta ccgggcggaa
     3841 attcgctgac atctgtcgcc accgcgtggc tgcagcgcag acgacgcgag gcggcacagg
     3901 tcttgataaa cgagatatcc aaagggcatc acggagaaat ttcgttcgag caacacgaca
     3961 tcgaccccct aatcgagatc atctatcgct tctcgaaggc cgtggcggat ggcgcggcag
     4021 tggacaatct tcgcttcttg gcgcaggtga ttgcagggct caagaaaa
//
LOCUS       DATMBB010000301         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_170452, whole genome shotgun sequence.
ACCESSION   DATMBB010000301 DATMBB010000000
VERSION     DATMBB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170452"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..514)
                     /locus_tag="VNL92_06440"
     CDS             complement(329..514)
                     /locus_tag="VNL92_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HWO94386.1"
                     /translation="MPIIRVEMWKGRTKDQKAELARAITDAMVAIAQTSPESTIIIFD
                     DVERENWAENGRLAIDE"
     gene            669..1394
                     /locus_tag="VNL92_06445"
     CDS             669..1394
                     /locus_tag="VNL92_06445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013270439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWO94387.1"
                     /translation="MASKTPTRSIVPTLDVESALWADGRSLVCGVDEVGRGALAGPVV
                     AAAVVLPRHAPAWLFDLRDSKALLPQERERLAALIRAQACCWSIGSAAPAVIDSVNIL
                     QATFVAMERAVAGLRPCADVALVDGSQLPRLPCETIGMVDGDAYVASIAAASILAKVA
                     RDGLMTALDRRYPGYGFAAHKGYSCPSHRRALLDMGVTPVHRRTFASVRSRLCGDGYL
                     EHLVALDITSPNGRGKQPGSGEE"
     gene            1391..1762
                     /locus_tag="VNL92_06450"
     CDS             1391..1762
                     /locus_tag="VNL92_06450"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWO94388.1"
                     /translation="MTNPRALGAAGERLAAAHLEAKGYRVEARNVRLPEGEIDIVASD
                     GHTRAIVEVRARRGTALGDGLDSVNERKAARLRQLAEAYAASTGYVGDLRIDVIAVDV
                     ATDGRLLGLRHLENAVEGDPA"
     gene            1759..2517
                     /locus_tag="VNL92_06455"
     CDS             1759..2517
                     /locus_tag="VNL92_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWO94389.1"
                     /translation="MTSAARVFRVSPFRLNTFRRCRRLYKYRYVERLPTAPNAYDTTG
                     SNIHAALRELMLRAPEERTPARAADIVREQWQTNRAGFRGMADEARWRARAIDQAARF
                     AQLPEAEGTPLAVERYLEVDLTPRVRLFGRIDRIDEASNEGLHIIDYKTGRRPDEIDP
                     GQLQLYAMMVHRSEERPVTAATFLYLDDGTSWTAPPSALDHAAAANGVLETVEQIVSE
                     REYEPAVGPYCAFCDFQAICPARKETVGRRLAEA"
     gene            2535..2975
                     /locus_tag="VNL92_06460"
     CDS             2535..2975
                     /locus_tag="VNL92_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO94390.1"
                     /translation="MPFYEYRCRDCHATTTAFFRTIAAAQQGTRCDRCGSEATERLIS
                     RSFVVKGTGRFDDFDPDRALGGLEGSDPGSFARWARRMSEDLGESAGGERFRELAERA
                     EAGDDPIERVDPGHTLRYELSQQRAALGGTDEAGDEASADDFFP"
     gene            2986..3330
                     /locus_tag="VNL92_06465"
     CDS             2986..3330
                     /locus_tag="VNL92_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO94391.1"
                     /translation="MPIYEYRCQDCSHLTNVFVRSASASVSASCESCGSANLQRLMSR
                     VAYHKSEQSILEDYGEPGIDARPEDYKDPRQIGRWAERKFDEWGMEMPREAREMIDAA
                     REGEFPDPVKDL"
     gene            3330..>3810
                     /locus_tag="VNL92_06470"
     CDS             3330..>3810
                     /locus_tag="VNL92_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HWO94392.1"
                     /translation="MAKTPAAASRAPKHDIDALVAPAVSELTAKHEAREAALPRCREV
                     IQLSANSIRATHRAEFERAEELLFRAADILDSISETLRGHADVYHAGFVHDAQKEYAE
                     ASLTLAFVAERPLPGPSDLRIGAAAYLNGLAEAASELRRHLLDSLRHGDLSRCEAVLA
                     "
BASE COUNT          670 a         1332 c         1201 g          607 t
ORIGIN      
        1 ctttggagcc ccgcggtgcc acccttcttg gtccagcagg gcgacgacgt tgggcgcctt
       61 gctggagccc gctctcacgc tataacgggc gatcccggcc gcgcctgcac cgcatactgc
      121 gggatcagcg tggcgactcc ggggcgagtt cgggctggtc catcgccggg cttccaccgc
      181 tgccccggct cgctgcgacg ggagcacaag ccctactgct ccccatcacc gtctgttcac
      241 ggcaatttga gaaaagtata gcgagcatat tgctcgatgg tcaacatcga ttgaaggggc
      301 gaatcctcga cctcaccccc acgcgcccct actcgtcgat ggcgagacga ccgttctccg
      361 cccagttctc ccgctccacg tcgtcgaaga tgatgatcgt gctctcggga gacgtctgag
      421 ctatagcgac catcgcatcg gttatggcac gggccagctc cgccttctgg tccttcgtgc
      481 gccccttcca catctcgacg cggataatcg gcatcgccct tccctcctac gccaatggtg
      541 tcagcgagca tagcaggcga cagcacggcg cgacagacga cgaccacggc agggggcttt
      601 ccagagcgta cgttctaggc tagtatccgc acatcggcgg atcgttccgc ggaatcccga
      661 ggcagagcat ggcttccaag acgccgaccc ggtccatcgt accaacgctc gacgttgaat
      721 ctgcgctctg ggccgatgga cgaagcctgg tctgcggcgt cgacgaggtc gggcggggag
      781 ccctggccgg ccctgtcgtt gcggcggccg tcgttctgcc acgccacgcg cctgcgtggc
      841 tgttcgacct ccgcgatagc aaggcgctgc tgccacagga gcgggagcga ttggcagccc
      901 tcatccgtgc ccaagcctgc tgctggagca tcggctcggc agccccagcc gtcatcgata
      961 gcgtgaacat cctgcaggcg accttcgtcg ccatggagcg cgccgtcgca gggctccgac
     1021 cctgcgccga cgtggcgctc gtcgatggtt cgcagctgcc gcgcctcccc tgcgaaacga
     1081 tcggcatggt cgatggcgac gcctacgtcg cctccatcgc cgccgcctcg atcctcgcca
     1141 aggtggcccg cgacgggctc atgacagcgc tggatcgacg ataccctggc tacggattcg
     1201 ccgcccacaa ggggtacagc tgcccatcgc accgccgcgc tctcctcgat atgggcgtca
     1261 ctcccgtgca ccggcgtacc ttcgcgtcgg tccggtcacg cttgtgcggc gacggctact
     1321 tggaacacct cgtcgccctc gacattacga gcccgaatgg gcgggggaag caacctggct
     1381 caggtgagga atgacgaacc ctcgtgccct cggcgccgcg ggggaacggc tcgctgccgc
     1441 ccacctcgag gcgaaaggct accgcgtgga agcgcgcaac gtccgcctgc cggaaggcga
     1501 gatcgatatc gtagcctccg atggccacac gcgagccatc gtcgaggtcc gcgctcgccg
     1561 gggcacagcg ctcggcgacg gactcgattc ggtcaacgaa cgtaaggccg cacgcttgcg
     1621 gcaactcgct gaggcctacg cagcaagcac cggctacgtt ggcgacctgc gcatcgacgt
     1681 catcgccgtg gacgtagcga ccgatgggcg gctcctgggg ctacgccacc tggagaatgc
     1741 agtggaggga gacccggcat gacttcggcg gcccgcgtgt tccgcgtctc gcccttccga
     1801 ctgaacacat tccgccgctg ccgtaggctg tacaagtacc gttacgtcga acggctcccc
     1861 acggcgccga acgcctacga tacgacgggc tcgaacatcc acgccgccct gcgcgagctc
     1921 atgctccgcg cacctgaaga acgcacgccc gctcgcgccg ccgacatcgt gcgggagcag
     1981 tggcaaacga atcgcgcggg cttccgcggg atggccgacg aagcacgttg gcgcgcgcga
     2041 gccatcgacc aggccgcccg gttcgctcaa ttgcccgagg cggagggaac gccgcttgcc
     2101 gtcgaacgct acctcgaggt cgacctgacg ccacgtgtgc ggctcttcgg cagaatcgat
     2161 cgcatcgatg aggccagcaa cgaaggcttg catatcatcg actacaagac agggcgccgc
     2221 cccgacgaaa tcgacccggg gcagcttcaa ctctatgcta tgatggtgca ccggagcgaa
     2281 gagcgtcccg ttacggccgc gacgttcctt tatctcgacg atggcacgtc gtggacagcg
     2341 ccaccatccg cgctcgacca cgctgcggca gccaacggcg tcctggagac cgtcgagcag
     2401 atagtgagtg aacgggagta cgagccggcc gtcggtccgt actgcgcctt ctgcgacttc
     2461 caggccatct gtcccgcaag gaaggagacg gtcggaaggc gcctcgccga ggcctgacac
     2521 gaggtcacgc aaacatgccg ttctacgaat accgatgtcg cgattgccac gccaccacga
     2581 ccgccttctt ccgcacgatt gcggctgcac agcaaggcac gcggtgtgac cgctgcggga
     2641 gcgaagcaac ggagcgcctc atctctcgaa gcttcgtcgt gaagggaacc ggtcgtttcg
     2701 atgacttcga ccccgaccgc gccctcggtg ggctcgaggg aagcgaccca ggcagtttcg
     2761 cccgctgggc ccgccgcatg agcgaggacc tcggcgagtc ggccgggggg gagcgctttc
     2821 gcgagctggc cgaacgagcc gaggcaggcg acgacccgat cgagcgcgtc gaccccgggc
     2881 acaccctgcg ctacgaactg tcgcagcagc gggccgcgct cggcggtact gacgaggccg
     2941 gcgacgaggc atctgcggac gatttctttc cgtgaggcca tcaccatgcc aatctacgaa
     3001 taccgctgcc aggactgtag ccacctcacg aacgtgttcg ttcggtcggc ttccgcaagc
     3061 gtctccgcat cctgcgagag ttgcggcagc gccaatctcc agcgcctcat gtcgcgcgtg
     3121 gcctaccata agtccgagca atcgatcctc gaggactacg gcgagcccgg tatcgacgct
     3181 cgccccgagg actacaagga cccgcggcaa atcggccggt gggcggagcg aaagttcgac
     3241 gagtggggaa tggagatgcc gcgcgaggcc cgcgagatga tcgatgccgc ccgcgaaggg
     3301 gagttccccg accccgtgaa ggacctctaa tggcgaagac ccctgccgcc gcgtcgcgcg
     3361 cccccaagca cgacatcgat gcgctcgtcg cgccggctgt cagcgaacta accgcgaagc
     3421 atgaggcgag agaggcagcc ctgccgcgct gccgcgaagt cattcaactc tccgccaact
     3481 ccatccgagc cacgcaccga gccgaattcg agcgagccga ggagctgctg tttcgcgccg
     3541 cagacatcct cgactccatt tcggagacct tgcgcggcca cgccgacgtc taccacgcgg
     3601 gcttcgttca cgacgcgcag aaggagtatg ccgaggcctc gctcacactc gctttcgtcg
     3661 ccgaacgacc gctccctggg ccgagcgatc tgcgcatcgg cgctgccgca tacctcaatg
     3721 gtctcgcgga agcagcgagc gagctgcgcc gccacctgct cgatagcctg cggcacggcg
     3781 atctgagccg gtgcgaggcg gttctcgccg
//
LOCUS       DATMBB010000302         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_439461, whole genome shotgun sequence.
ACCESSION   DATMBB010000302 DATMBB010000000
VERSION     DATMBB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439461"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VNL92_06475"
     CDS             complement(<1..619)
                     /locus_tag="VNL92_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94393.1"
                     /translation="MAIDVDTMEMLRKEPEVDLDLDWMKLMSDWGWRAQPGRKGLTLE
                     NVAEGTLGVVPESTRNYTGRPRGVVARPNAPRIGQPYPDRAETWADSIMMLYEEALAR
                     QWSSATDIPWETIEPLPDDLELAMCQLCTFLTEVEFIAGDIPGKWFPAISPEFFEVQL
                     FLGTQIMDEGRHLDVFRKRALANGGGLMEGLGFAAVGSIGSSDFTE"
     gene            complement(751..2553)
                     /locus_tag="VNL92_06480"
     CDS             complement(751..2553)
                     /locus_tag="VNL92_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94394.1"
                     /translation="MGSEYWERVRKRRVTRRRVLGAGALGASGAVLAATVGCGDDDDD
                     DDDAGSGTAAAGGERDTSQLPERFREARYGGILRFGVNDPPQTLDLHAHDSPGGHLVA
                     YPAYNGLIRRPENPPGNATSEPELVEEWEQPDETTTILHVRKGVKFHNVEPTNGREMT
                     GEDIVYTIERAQGKHPHSSQVGGEFRMRDMFPVNLELTDDYTVRLTSEKPFAPLLNNL
                     GFSWLMVEPRELVEATGDRSVITWAVGTGPFIMDGYDTLGDGGMVTHVRNPDYWRKGH
                     PFVDKIEQPIVTGLDTLQAMYLNDDVDTTIYIQSAIVKAMEEAGKEMQIFPVPAIGPL
                     KLYFDMANQDSPWTREQRLRQALHYLIPYDAILAGLDATCCRSGPMPPHYKPWALNES
                     ELPAAGMPFADAVQRGIELMTEAGFPPGTEIPLELSTSPFYSGTQIGEALVGVFGAIK
                     QQTNGAVNINARLNVMELTDWFQQIYFGGGTYMATCHGDWSWEEPDNAFYRYFHSTGV
                     ANNTHTNDAELDRLIEQQRQELDPEKRLQIVRDIQFRLLDTSQMIWIVSPEGFTGAQR
                     FIGGYSPMFLNNVESPRHFDELWLTEDAPSSRRT"
     gene            complement(2726..>3884)
                     /locus_tag="VNL92_06485"
     CDS             complement(2726..>3884)
                     /locus_tag="VNL92_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HWO94395.1"
                     /translation="QALLGHRLRLRQWDDFFQNLAVYAYFAGARRLPPPDDGHIFIES
                     YPGGWFWCIPLHTGVSSVGAVVDSRVGQDGMRKAGGAEPFLRAQIALAPGIAQMLSDA
                     RLESGPFVVKDWSYVSTEVAGPGYVLAGDAACFADPLFSSGVHLALTAGLLSAAYVAT
                     VLRDETMREPAAGVYKDLYYRQYGYYHEMAKLFYSSNRSVESYFWEARRILGGDGRYT
                     PRESFIRAVAGQPPQGYERAVIEHGDAPEDFRTTVEAARTDLAERRAHIEAAMRPVPG
                     SKEAALLAAVPRLAEGVSLVRRPVLGSGEFVWGRVVVSPARPDGVPVIDPVAHLLQAI
                     DGRTPVRDLVRRMLATVRREDRPGAERAVLMTLRDLFVEGAIELRGPQGRA"
BASE COUNT          654 a         1281 c         1216 g          733 t
ORIGIN      
        1 cttccgtaaa gtcgcttgag ccgatgctgc cgactgctgc gaagcctagc ccttccatca
       61 ggccgccgcc gttggcgagt gcccgcttgc ggaacacgtc gaggtggcgt ccctcgtcca
      121 tgatctgggt gccgaggaac aactggacct cgaagaactc ggggctgatg gccgggaacc
      181 acttgcccgg gatgtcgcca gcgatgaact cgacctcggt gaggaaggtg cacagctggc
      241 acatcgccaa ctcgaggtcg tcgggcagcg gctcgatcgt ctcccacggg atgtcggttg
      301 cggaggacca ctgacgtgcg agggcttcct cgtacagcat catgatgctg tcggcccacg
      361 tctctgccct atcgggatac ggctgtccga tgcgcggtgc gttgggccga gcgacgacgc
      421 cgcggggcct gccggtgtag ttgcgcgtgc tctccggcac gacgcccaac gtgccttcgg
      481 cgacgttttc gagcgtgagg cccttccgcc cgggctgggc tcgccagccc caatcgctca
      541 tgagcttcat ccaatcgagg tcgagatcga cctccggctc cttgcggagc atctccatgg
      601 tgtcgacatc tatcgccatt cttccctcct tgcggtcgcc ttcgaacgac ggccggcgaa
      661 acacgtacca accggttgtt tgaatctagt gcagacgtca gcacatgtca actttaccgc
      721 gcctgtcgtt gcattcgcgc atcgatagat ttacgttctg cggctgctcg gcgcgtcctc
      781 ggtcagccaa agctcgtcga agtgcctggg cgactcaacg ttgttgagga acatgggcga
      841 gtagccgcca atgaagcgtt gcgcgcccgt gaatccttca ggtgagacta tccagatcat
      901 ttgtgacgtg tcgagcagcc ggaattgaat atcccgcacg atttgcaagc gcttctccgg
      961 gtccagctcc tgcctttgct gctcgatcag ccggtcgagc tctgcgtcgt tcgtgtgcgt
     1021 gttgttcgcg acgccggtcg aatggaagta gcggtagaag gcgttgtcgg gctcctccca
     1081 cgaccaatcg ccgtggcatg tggccatata ggtgccgccg ccgaagtaga tctgctggaa
     1141 ccagtcggtc agttccatca cgttcagccg cgcgttgata ttcacggccc cgttggtttg
     1201 ctgcttgatg gccccgaaca cgcctacgag ggcctcgccg atttgcgtgc cggagtagaa
     1261 cggcgacgtg ctcagctcca ggggaatctc cgttccgggc gggaagccgg cctcggtcat
     1321 cagttcaatg cccctttgca ccgcgtccgc gaacggcata ccggcggcgg gcaattcgct
     1381 ctcgttcagg gcccatggct tgtagtgggg cggcatgggc ccggagcggc agcaagtagc
     1441 atcgagcccg gcgaggatgg catcgtacgg gatcagatag tgcagcgcct gtcgcagccg
     1501 ctgctcgcgc gtccacgggg aatcctgatt ggccatatcg aagtagagct tgagagggcc
     1561 gatggccggg acggggaaga tctgcatctc cttccccgcc tcctccatcg ccttgacgat
     1621 ggcagactgg atgtagatcg tcgtatcaac gtcgtcattg aggtacatgg cttgcagcgt
     1681 gtcgaggccc gtgacgatgg gctgctcgat cttatccacg aaggggtggc ccttcctcca
     1741 gtaatcgggg ttgcggacat gcgtgaccat gccgccatcg ccgagcgtgt cgtacccatc
     1801 cataatgaac ggtcccgttc cgacggccca cgtgatcacg ctgcggtcgc ccgtcgcctc
     1861 gacgagctcg cgcggctcca ccatcagcca ggagaatccg aggttgttca gtagcggcgc
     1921 gaagggcttc tccgatgtca gccggaccgt gtagtcgtcg gtcagctcga ggttcacggg
     1981 gaacatgtcg cgcatgcgga actcgccccc aacctggctc gagtggggat gtttgccttg
     2041 cgctcgttcg atcgtataga cgatgtcctc gccggtcatc tcccggccgt tcgtcggctc
     2101 gacgttgtgg aacttgaccc ccttgcgcac gtgaaggatc gtcgtcgtct cgtcgggctg
     2161 ctcccactct tcgacaagct cgggctccga cgtagcgttt cccggcgggt tctccggcct
     2221 ccggataagg ccgttatacg ccggataagc gacgaggtga ccgccgggcg aatcgtgcgc
     2281 gtggaggtcg agcgtctgag gtggatcgtt cacgccgaag cgcaggatgc cgccgtaccg
     2341 ggcctcgcgg aagcgctccg gaagctgcga ggtatctcgt tctccgccgg cagctgccgt
     2401 gccgctcccc gcgtcgtcat cgtcatcgtc gtcatcgccg caaccgaccg tagcggccag
     2461 gaccgcgccc gatgcgccaa gagcgccggc gcccaacacc cttcgccgcg taacgcgtcg
     2521 cttgcgcacg cgttcccagt actcggatcc catctcatcc ccctcagaga tccttggcac
     2581 gtgccagacc ctagtggtgc tgtaacctac ccgatgcgag agaatcgcgt caactcgatc
     2641 gagaccactc gattcctccc attatcgcag cgctcctcga ttcctgttgc gttgcacagc
     2701 cttggcgcaa gcgcgcgctc tgcggttatg cgcgaccctg aggcccccga agctcgatcg
     2761 cgccctcgac aaacaggtcg cgcaacgtca tgaggaccgc ccgttcggcg cccggccggt
     2821 cttcccgtcg cacggtggcc agcattcgtc gcacgagatc gcgcaccgga gtgcgcccat
     2881 cgattgcctg aagcaggtgg gcaaccgggt cgatgaccgg cacgccatcc ggccgcgccg
     2941 gcgaaaccac gactcgaccc catacgaact ctccgctgcc caacaccgga cggcggacga
     3001 gcgatacgcc ctcggcgagc cgaggcacgg ccgcgagcaa ggctgcctcc ttcgaaccag
     3061 ggacgggtcg catcgccgcc tcgatgtgag ctcggcgctc cgcgaggtcg gttcgcgcgg
     3121 cttcgacggt ggtcctgaag tcttccggcg cgtcgccgtg ttcgatgacg gcgcgctcgt
     3181 agccctgcgg tggctggccg gccacggctc gaataaacga ctcacggggc gtgtatcgac
     3241 cgtcgccacc gagaatgcga cgggcctccc agaagtacga ttcgacgctt cggttgctgg
     3301 aatagaacag cttggccatc tcgtggtagt agccgtactg acggtaatac aggtccttgt
     3361 agacgcctgc cgccggttcg cgcattgttt cgtcgcggag caccgtcgcg acgtaggcgg
     3421 ccgagagcaa gccggccgtg agggccagat gcacgcccga cgagaacagc ggatcggcga
     3481 agcacgcggc atcgcccgcg agcacatatc ccggtcccgc cacctccgtg gagacgtacg
     3541 accagtcctt tacgacgaac ggtccggatt cgagccgcgc atcgctgagc atctgagcga
     3601 tgcccggcgc cagggcaatt tgtgctctga ggaatggctc ggcgccgccc gccttccgca
     3661 tgccatcttg gcctacccgg ctatcgacca cggcgcccac gctcgataca cccgtgtgca
     3721 gcggaatgca ccagaaccaa ccaccagggt acgactcgat gaagatatgg ccatcgtcgg
     3781 ggggtggcag ccgccgcgcc cccgcgaagt aggcgtacac cgccaggttc tggaaaaagt
     3841 cgtcccactg ccgcagccgc aggcgatgcc cgagcagcgc ctgc
//
LOCUS       DATMBB010000303         2494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_484874, whole genome shotgun sequence.
ACCESSION   DATMBB010000303 DATMBB010000000
VERSION     DATMBB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484874"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VNL92_06490"
     CDS             <1..583
                     /locus_tag="VNL92_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94396.1"
                     /translation="PGYGIYECADGHVMFMVGIAGFGAGWPVLINWMQEEGAAEDLAD
                     PKYLELFENLDMRTLTNLLMGGGDPQTVQEMMQRFAHINEVLTAFIKGRSKQYLYEEG
                     QARRLLVGIVSTPKDLYENPQLRAHDFWAEVEHPELGPTFEYPGAPYHIPKAPWRIRR
                     RAPLLGEHTRSVLCEELGLTDEDIVTLSAAGIT"
     gene            645..1871
                     /locus_tag="VNL92_06495"
     CDS             645..1871
                     /locus_tag="VNL92_06495"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94397.1"
                     /translation="MTSTKLPLEGIRVADFSWFGAGPIAGAILANFGAEVIRVESETH
                     VDGLRVAQPVADGKTGYNVSGYYNNFNAGKLSFTLNLSHPMSRDIALRLIAKCDIVME
                     NYAPRVFEKWGLTYDAMREVNPSVIFLREPMMGTWGPHRDFAGFGAVLSPIAGLSHLS
                     GYADRTPIGIGTNYSDYVINPGHAALAMLAALRHRQRTGEGQQIELSQLESTAAVFGQ
                     ALMEYSVNRHVRTREGNKQPNAAPHNAYPCAGEDRWIVIACFTDDEWSALVSEMGQPE
                     WARSDKFGTLLGRKRNEEELDRHVAEWTKTQDEYELMHRLQAAGVPAGVVQNAQDVLE
                     RDVNLRERGYFWYLDHPEAGRTAYDGPGFRLSRTPGRLGGPAPCLGAHTAQICQDIIG
                     MDDDEMSEMVMAGVLQ"
     gene            complement(1885..2118)
                     /locus_tag="VNL92_06500"
     CDS             complement(1885..2118)
                     /locus_tag="VNL92_06500"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWO94398.1"
                     /translation="MASAGLVRLVAAADEVEAERMRRALLAAGIRSVVRNVDPLSVMH
                     RLPPPPFSLVLFVREDELEEARTLLGLEEGLAG"
     gene            complement(2123..>2494)
                     /locus_tag="VNL92_06505"
     CDS             complement(2123..>2494)
                     /locus_tag="VNL92_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWO94399.1"
                     /translation="QRLLEGPPIDVVIETVGGEADTFMQALTVARRGGRVGVLGAFTA
                     ESPFNPSIIMVKELTVVGGLTYGRPDPYADFDFAVDIVSKHRETLGRLITHRIGLERI
                     QEAFETAADKSRKTIKVSVEA"
BASE COUNT          477 a          830 c          798 g          389 t
ORIGIN      
        1 gcccggctac ggcatctacg agtgcgccga tggccacgtc atgttcatgg tcggcattgc
       61 aggcttcggt gcgggctggc ccgtgctcat caactggatg caggaggaag gagccgccga
      121 ggacctcgcc gaccccaagt acctcgagct gttcgagaac ctcgacatgc gcacgctcac
      181 caacctcctg atgggcggcg gcgacccgca gaccgtgcag gagatgatgc agcggttcgc
      241 acacatcaac gaggtcctca cagccttcat caagggtcgc agcaagcaat atctgtacga
      301 ggaaggccaa gcgcggcgcc tcctcgtggg catcgtcagc acgccgaagg acctgtacga
      361 gaaccctcag ctccgcgctc acgacttctg ggccgaagtc gaacacccgg agctggggcc
      421 cacgttcgag tatccgggag cgccgtacca tatcccaaag gcgccctggc gaatccgccg
      481 ccgcgccccc ctactcggcg agcatacccg cagcgtcctc tgcgaggagc tggggctgac
      541 cgacgaggac attgtcacct tgagcgcggc aggcatcacg tgatccatga cgttccgcca
      601 gtacatcgcg tccatcgccc gatcgagaca agggggaggt acgtatgact agcacgaagc
      661 tgccactcga aggcatccgc gtcgccgact tcagctggtt cggcgccggg ccgatcgcgg
      721 gggccatcct cgcgaacttc ggcgccgagg ttatccgcgt cgagtcggag acacacgtcg
      781 acggcctgcg ggtcgcccag cccgtcgccg atgggaagac cgggtacaac gttagtggct
      841 actacaacaa cttcaacgcc ggcaagctct cgtttacttt gaacctcagc cacccgatgt
      901 cgcgcgacat cgcccttcgg ctcatcgcca agtgcgatat cgtcatggag aactacgctc
      961 cgcgcgtgtt cgagaagtgg gggctgacgt acgatgcgat gcgcgaggtg aatccgagcg
     1021 tcatcttcct gcgcgaaccg atgatgggca cgtgggggcc gcatcgcgat ttcgccgggt
     1081 tcggcgccgt cctcagcccc atcgctggcc tcagtcacct gagcggatac gccgaccgaa
     1141 cgcccatcgg cattgggacg aactactccg actacgtgat caacccaggc catgcggcgc
     1201 tggcgatgct cgcggcgttg cgccaccgtc agcgcacggg cgagggccag cagatcgagc
     1261 tctcgcaact cgaatcgacc gccgcggtgt ttgggcaggc gttgatggag tacagcgtca
     1321 accgccatgt ccgtacgcgg gaaggcaaca agcagccgaa cgcggcgccc cacaacgcct
     1381 atccctgcgc cggcgaggac cgatggatcg tcatcgcgtg cttcacggac gacgaatgga
     1441 gcgcactcgt aagcgagatg ggtcagccgg aatgggcccg cagcgacaag ttcggcacgc
     1501 tcctcggccg caagcgaaac gaagaggagc tcgaccggca cgtcgcggag tggacgaaga
     1561 cgcaagacga gtacgagctt atgcaccggt tgcaggcggc cggcgtgccc gccggcgtgg
     1621 ttcagaacgc gcaggacgtc ctcgagcgtg acgtgaacct tcgggaacgt ggttacttct
     1681 ggtacctcga ccaccccgaa gcgggacgca cggcttacga tggcccgggc ttccgcctct
     1741 cgcgcacgcc gggccggctc ggaggacctg ctccctgtct cggcgcccac acggcccaaa
     1801 tatgccagga tatcatcggc atggacgatg atgagatgtc ggagatggtc atggccggcg
     1861 tcctgcaata ggacgaaggc cgagctagcc ggccaatccc tcctccaggc ctagcagggt
     1921 gcgcgcctcc tccagctcgt cctcccgaac gaagaggacg aggctgaacg gcggcggcgg
     1981 caggcgatgc atcaccgaga gaggatcgac gttccggacg acggaccgaa tcccggcggc
     2041 gaggagtgcg cgtcgcatcc gctcggcctc aacctcgtcg gccgcggcga cgagccgcac
     2101 gagacccgcg gaagccacgc aatcaggcct cgacgctcac cttgatcgtc ttccgcgact
     2161 tgtcggcggc cgtctcgaac gcctcctgga ttcgctcaag cccgatacgg tgagtgatga
     2221 ggcgtcccaa ggtctcacga tgcttcgata cgatgtccac ggcgaaatcg aagtccgcgt
     2281 acgggtcggg ccggccgtag gtcaaccctc cgacgacggt tagctccttg accatgatga
     2341 tggagggatt gaagggcgac tccgcggtga acgcaccgag cacgccgacc ctcccgccgc
     2401 gtcgagcgac ggtgagagcc tgcatgaagg tatcggcctc gccgccgacc gtctcgatga
     2461 ccacgtcgat gggcggcccc tcgagcagcc tctg
//
LOCUS       DATMBB010000304        11612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_170622, whole genome shotgun sequence.
ACCESSION   DATMBB010000304 DATMBB010000000
VERSION     DATMBB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170622"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VNL92_06510"
     CDS             complement(<1..1418)
                     /locus_tag="VNL92_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HWO94400.1"
                     /translation="MTQAAERTEGIAEKTLRTLEYDKVLERLAAHTSFSLGRALALAS
                     RPTTVHAEVVRLQRMTAEARHLATQRGFSLGGAKDVRSLVEKAALGGVLDPGELLDIR
                     GTLEAAAAVQSFVQRTAGMTPLLTDLVDEIEPLPSIVTEIARSIDERAEVMDAASPAL
                     GALRREVRSLHDTLVARVESIMRDSFDRGVAQEPIVTQRDGRYVIPVKADARGQLPGV
                     VHDVSASGATVFVEPLAVIELANRWREAQLEERREVERVLRRLSADVGSETSAIVRNI
                     EILGELDVAIARARYAEELHAPLPSLNDGPVWVVEAPAELRLLEARHPLLSGHVVPVT
                     ILVGSEDARILLITGPNTGGKTVALKTAGLLVLMAQSGMGVPAEEGSQLPVYESVLAD
                     IGDEQSIEQSLSTFSGHISNIVQILAQAGPPSLVLLDELAAGTDPAEGAPLARAILTE
                     LLERRATVLATTHHGELKTFAH"
     gene            complement(1552..2088)
                     /locus_tag="VNL92_06515"
     CDS             complement(1552..2088)
                     /locus_tag="VNL92_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO94401.1"
                     /translation="MKLLVPIDQTTLSDSILRVVERTVLPMIDEVHLLAVLNKRNVKR
                     AKRGEEMEWSHEMAPREGAWAIYPLYLSEDQIPGVLPEGPERQISDDDLRVNARSYLV
                     SAASRFADDVEIVPVVIEDQDPPKAIIEYGRQERVDLIAMATHARSGLSRAVLGSVTD
                     EVIRSAIAPVLVCASADR"
     gene            complement(2189..3805)
                     /gene="cimA"
                     /locus_tag="VNL92_06520"
     CDS             complement(2189..3805)
                     /gene="cimA"
                     /locus_tag="VNL92_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HWO94402.1"
                     /translation="MNVELYDTTLRDGAGMEGISLSVEDKIKIARKLDSLGIHYIEGG
                     YPGSNQKDAEFFARASAIGLRNATLVAFGSTRRAGARVGDDANLRALLESGATHVTLV
                     GKAWDRQVEEVLETSLEENLAMIADSIEYLRAEGRTVFFDAEHFFDGFRGNPDYALQC
                     LRTAARAGARVSVLCDTNGGTMPEALVAAIRAAQEATPGHALGIHVHNDADLAVANSL
                     IAFEAGAVQVQGCANGYGERCGNANIFSIIANLKLKMGVDCVSDDQLARLTEVSHYIS
                     EIANIAPDPHQPYVGASAFAHKAGLHVAALTKSADSYHHVPPERVGNDSRILVSEFSG
                     RRNILLKMHEQGIDLPLSSEEARELLDRVKVMEARGFQYDGAEASFELLARRRMPDYR
                     APFSLEDFLVVERRRHRAENGEGDHNDMLAEAMVKVRVGDEVQHTAAEGNGPVNALDQ
                     ATRKALVQFYPGIASVRLVDYKVRIIDTGAGTGAAVRVLIESTDGDNTWRTVGSSTDI
                     IEASWLALADSLEYWLTKHAVPGAPIVPLT"
     gene            complement(3948..5039)
                     /gene="leuB"
                     /locus_tag="VNL92_06525"
     CDS             complement(3948..5039)
                     /gene="leuB"
                     /locus_tag="VNL92_06525"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWO94403.1"
                     /translation="MTRRIVVLPGDGIGPEVTAEGIKILRAVERRFGLRFEFEQDAVG
                     GASIDAHGTALRSETLERCRRAEAVLFGAVGGPKWDDPAAPVRPEDAILGLRKGLGLF
                     ANIRPVRTQSFLCASGPLRPEVVEGVDLVVIRELTGGIYFGRPKRRWRNASGRQAVDT
                     LRYNEREVARIVRVAFEMARTRRRKVTSVDKANVMESGRLWREVTSEVARDYPDVALE
                     HMLVDACAMHLIRRPGDFDVIVTENLFGDILTDEASVLAGSMGLLPSASLGRRRRDGT
                     GMGLYEPIHGSAPDIAGQGRANPIAMILSVVMMLRLSFGEEEAARAVEHAVDRALASG
                     RRTPDIASEGEAVVATSEMGDLVAAALQD"
     gene            complement(5029..5523)
                     /locus_tag="VNL92_06530"
     CDS             complement(5029..5523)
                     /locus_tag="VNL92_06530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO94404.1"
                     /translation="MVKVQLGVTVREASGSDVPAVLAITRAAFEGYREEMPDYGALHE
                     TEDDIRGWLERGMVLLAEQDGVMVGSVRLAQLEDGDWYVGRLGIVPDAPGWVGSRLMR
                     EAEARAREHGGKAVRLGVLASRHRLRRYYERRGYVLTDVYRPDEAPDHPGWAWYRKDL
                     LGDA"
     gene            complement(5590..6195)
                     /gene="leuD"
                     /locus_tag="VNL92_06535"
     CDS             complement(5590..6195)
                     /gene="leuD"
                     /locus_tag="VNL92_06535"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWO94405.1"
                     /translation="MEPFTVVTGYVAPLDRNDVDTDQIIPKQFLKRIERTGFGEFLFF
                     DWARLPDGSPNPDFVLCQPYYREAKILVTGRNFGCGSSREHAPWALSQFGIRVIIAPS
                     FADIFRNNCYQNGMLPLELPQDVVDTLMARAIERPGYEATVDLERQEVRDHNGVVTRF
                     AIDPFVRERLLHGLDDIGLTLQHEHAIAAYERRREQELATA"
     gene            complement(6198..7610)
                     /gene="leuC"
                     /locus_tag="VNL92_06540"
     CDS             complement(6198..7610)
                     /gene="leuC"
                     /locus_tag="VNL92_06540"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWO94406.1"
                     /translation="MQPKTLFDKIWDAHVVYEEPGRPTLLYIDLHLVHEVTSPQAFEG
                     LRLAGRNVRRPDLTLATVDHNVPTTDRSLPIEDPISRQQVEVLVRNAQDFGITVFDYH
                     SQYQGIVHIIAPEQGRTQPGMTIVCGDSHTSTHGAFGAFAMGIGTSEVEHVLATQTLP
                     VAKPKTMEVRVDGVPPYGVSAKDVALGIIRAIGTSGGAGYVIEYTGDAIRSLSMEGRM
                     TVCNMSIEAGARAGMIAPDDVTFDYLRGRMYAPQGEAFERAVQRWRRLPTDPGATFDR
                     TVALDASTMEPFVTWGTTPAQSVPVTGRVPHPADTPDPSGTERALEYMGLRPGTRMQD
                     LAVDRVFLGSCTNARIEDLRAAAAIVKGRKVAPSVRAMVVPGSMLVKAQAEREGLDRI
                     FREAGFEWREAGCSMCLGMNPDVLQEGERCASTSNRNFEGRQGKGGRTHLVSPQMAAA
                     AAIAGHFVDIREWDLAREVA"
     gene            complement(7713..9266)
                     /locus_tag="VNL92_06545"
     CDS             complement(7713..9266)
                     /locus_tag="VNL92_06545"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWO94407.1"
                     /translation="MTDNRVLIFDTTLRDGEQSPGVALNATDKLEIAHQLQRLGVDYI
                     EAGFPNSSPGDFEAVTAIAKEVRGCAIAGLARAHPADIDACWDAVRHAEHPRIHVFVS
                     SSDIHQMHQLKKDRDEVLELARTMVARAKKYTDDVEFSPMDATRSEREYVYRMLTECI
                     EAGAGTVNIPDTVGYAIPEEFEQFLREIQENVPNIHRARISVHCHNDLGLSTANSLAA
                     VRAGARQIECTINGIGERGGNASLEEVVMALKTRRDYFGVDVGVKTREIYRTSRLVSD
                     LTGMVVQPNKAIVGANAFRHQSGIHQDGILKMRETYEIMDAHEIGWPSGGAEVVLGKL
                     SGRHGFKARLEELGYELTDEEMSRAFLAFKELADKKAQIDDRDLEALVADKLRTTQEV
                     YRLDLVQVTAGDHTTPTATVRLVAPDGRVLSDAALGTGPVDAVYRAINRIVQIDNRLT
                     EFSVKSVTEGIDAQGEVTIRIEMNGRTYVGRGADTDIIVASAKAYMNALNRALSVHRP
                     TDAEVRTNP"
     gene            complement(9452..10444)
                     /gene="ilvC"
                     /locus_tag="VNL92_06550"
     CDS             complement(9452..10444)
                     /gene="ilvC"
                     /locus_tag="VNL92_06550"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWO94408.1"
                     /translation="MAQMYYEKDADLGVLKGKRIGVIGYGSQGHAHALNLKDSGCDVA
                     VGLYPGSKSWKKAEDDGLRVASVNDVAEASDLIMILAPDQVQRELYEKGIEPHMRSGK
                     MLMFAHGFNIHYGQIQPPADVDVAMVAPKGPGHLVRRVFVEGGGVPALFAVHQNASEQ
                     ARQLTLAYAKGIGALRAGGLETTFKEETETDLFGEQAVLCGGLTSLVKNAFETLVSAG
                     YQPELAYFECMHELKLIIDLMYEGGMSYMRYSISDTAEDGDYTSGPKVIDGHVRDNME
                     QVLSRIQDGTFAREWILENQAGRPSFMAMRRRDAEHPIEQIGQELRAMMAWLKA"
     gene            complement(10466..11089)
                     /gene="ilvN"
                     /locus_tag="VNL92_06555"
     CDS             complement(10466..11089)
                     /gene="ilvN"
                     /locus_tag="VNL92_06555"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWO94409.1"
                     /translation="MTTRNVTRNGARPRQSLHTIVALVEDQPGVLNRVASLFRRRGFN
                     IESLAVGHTETPGISRMTLVVDGAKTVIEQVEKQLYKLIDVVKVADITHDPIVARELA
                     LVKVRCNTRSRSEIIQIAQVFRADVVDVAETSLIIQVAGGEDKVDAFLQAVKPYGIRE
                     VVRTGRIALTRGAVGDTAVTEVEPQDVREFHAHAGRRPKGALPYVSD"
     gene            complement(11086..>11612)
                     /locus_tag="VNL92_06560"
     CDS             complement(11086..>11612)
                     /locus_tag="VNL92_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO94410.1"
                     /translation="AAQHYQFRKPRRFVSSGGLGTMGFEVPAAMGAQAADPSAEVWSI
                     AGDGGFQMTMQELATIIDEKQPIKFAIMNNGYLGMVRQWQELFYKNNLVDVRIAQPDF
                     VKLAEAYGIPALRVEDKGDVRAAIDEARAYQGPFIIDFRVEEEGNVWPMVPAGASLFE
                     TIEGVEDLLGSTVS"
BASE COUNT         1986 a         3913 c         3600 g         2113 t
ORIGIN      
        1 tcgtgtgcga acgtctttag ttcaccgtgg tgcgtcgtcg ccagcacggt ggcgcgccgc
       61 tcgagcagct ccgtcaggat ggcacgggct aggggcgccc cctcggcggg atccgtgccc
      121 gccgccagct catcgagcag aacgaggctg ggaggtccgg cctgcgccag gatctggacg
      181 atgttgctga tgtgcccgct aaacgtcgaa agcgactgct cgatgctctg ctcgtccccg
      241 atgtccgcga gcaccgactc gtacacgggc agttgggagc cctcctcagc cggcacaccc
      301 atgcccgatt gggccatgag cacgagcagg ccggctgtct tgagcgcgac ggtcttgccg
      361 ccagtgttgg gaccggtgat gaggagaata cgagcgtcct cgctgcccac gagaatcgtg
      421 accggaacaa cgtggcccga tagcagggga tggcgcgcct cgagcagacg cagctcggcc
      481 ggcgcctcga ctacccaaac ggggccatca ttgagggaag gcaggggggc atggagctcc
      541 tccgcatagc gggctcgtgc gatggccacg tcgagctcgc ccaggatctc gatgttccgg
      601 acgatcgcgc tggtttccga gccgacgtct gcgctcagcc tgcgcagcac ccgctcgacc
      661 tcgcgtcgct cctcgagctg cgcttcgcgc cagcgattcg caagctcgat gacggcgagc
      721 ggctcgacga acacggtagc gcccgaggcc gagacatcgt gcacgacgcc tgggagctgt
      781 ccccgtgcat cggccttcac cggaatgacg taccgaccgt cccgctgtgt gacgataggc
      841 tcctgagcta cgcctctgtc gaaggaatcg cgcatgatgc tttcgacgcg ggccaccagc
      901 gtgtcgtgaa gggagcgaac ttcgcggcgc agcgctccca gcgcggggct cgcggcatcc
      961 atcacctcgg cgcgctcgtc gatcgaccga gcgatctcgg tcacgatgga cggcagcggc
     1021 tcgatctcgt cgacgagatc ggtgaggagg ggtgtcatgc cggcagttcg ttgcacgaag
     1081 gactgcacgg ccgctgccgc ctccagcgtc ccgcgaatat ccagcagctc gcccgggtcc
     1141 agcacgccac ccagagccgc cttctcgacc aacgaccgca cgtccttcgc cccgccgagc
     1201 gaaaagccgc gttgcgttgc gagatgtcgc gcctccgcgg tcatgcgctg caaccgaaca
     1261 acctcggcat gaacggtggt cggccgcgag gcaagcgcga gcgcccgccc cagcgagaaa
     1321 ctcgtatgcg cggccaatcg ctccagcacc ttgtcgtact ccaaggtgcg gagcgtcttt
     1381 tcggcaatgc cttcggtgcg ttcagccgcc tgcgtcatgc gcccttcacg ctgcgatctc
     1441 tttcccgcta gcatagcgag ccctgcggcg gactgcgagc ggaggcgtcc tcgactgcga
     1501 agcagacgaa agcggcagtg agagcggagg gatgttgggc gcgagaactc gctagcggtc
     1561 ggccgacgcg caaacgagca cgggcgcaat tgccgagcgg atgacctcgt cggtcacgct
     1621 gcccaaaacg gcgcgggaca gaccactgcg agcgtgtgtt gccatcgcga tgaggtccac
     1681 gcgctcttgc cggccatact cgatgatcgc cttcggtggg tcttgatctt cgatcacgac
     1741 gggcacgatt tcgacgtcgt cggcaaaccg cgaggcggcg ctaacgaggt aggaccttgc
     1801 gttcacacgc aaatcgtcgt cgctgatctg gcgctcgggg ccttccggga ggactcccgg
     1861 aatctgatct tcagagagat agaggggata gatggcccag gccccttctc gcggcgccat
     1921 ttcgtgggac cactccattt cctcgccgcg cttcgctctc ttcacgttcc gcttgttgag
     1981 gacagcgagg aggtgaacct catcgatcat ggggagcacg gtgcgctcga caaccctcaa
     2041 gatcgaatcg gacagcgtcg tttgatcgat tgggaccagc aacttcattc gaccgactcc
     2101 ccgaggctga tggctgctat gcgttcaacc tatcaccacc aacaactcgt gacaccttct
     2161 cgcaagggaa ttgcaacaac cgcgcccgtt atgtgagagg gacgatagga gcaccgggga
     2221 cggcatgctt cgtgagccaa tactcgagac tatcggcaag agcaagccag ctcgcctcga
     2281 tgatgtcggt agagctgccg accgtgcgcc acgtattgtc cccatcggtc gactcgatga
     2341 gcactcgcac cgcggcccct gtcccggcgc ccgtatcgat gatgcgcacc ttgtagtcga
     2401 cgagccgcac gctggcgatg cccgggtaga actgcacaag cgccttccgc gtcgcctggt
     2461 cgagcgcgtt caccggcccg ttgccttcag cagccgtatg ctgcacctcg tcgccgacgc
     2521 gcaccttgac catcgcctcc gccagcatgt cgttgtggtc gccttccccg ttctctgcgc
     2581 ggtggcgcct gcgttcgacg acgaggaaat cctcgagaga gaagggtgcg cggtagtccg
     2641 gcatgcgccg gcgcgccagc agctcgaacg acgcctcggc tccgtcgtac tggaaaccac
     2701 gcgcctccat gaccttgacg cggtcgagca gttcgcgcgc ttcctcggag ctgagtggca
     2761 gatcgatgcc ctgctcgtgc atcttcaaca ggatgttgcg ccggcctgaa aactcggaaa
     2821 ccaggatgcg gctgtcgttt ccgactcgct ccgggggaac gtgatggtag gagtccgcac
     2881 tcttggtgag cgcggcgaca tgcaggccgg ccttgtgcgc aaaggcgctc gctccgacat
     2941 agggttggtg tgggtccggc gcgatgttcg cgatttcgga gatgtagtgg gaaacctcgg
     3001 tgagtcttgc tagctgatcg tcgctcacgc aatcgacgcc catcttgagc ttgagattgg
     3061 cgatgatgct gaagatgttg gcgttgccgc aacgttcgcc gtagccattg gcacagccct
     3121 gcacctggac ggcaccggcc tcgaaagcaa taagcgaatt cgcgacggcc aggtcggcgt
     3181 cattatgcac gtggatgccg agagcgtgcc caggagtggc ctcctgcgcc gcacgaatcg
     3241 cggcgacgag cgcctccggc atcgtgccgc cgttcgtgtc gcacaagacg gagacgcggg
     3301 cgcctgctcg ggcggcggta cgcaggcact ggagggcgta gtcggggttt ccccggaagc
     3361 catcgaagaa gtgttccgcg tcgaagaaga ccgtacggcc ctcggcgcgc aggtactcga
     3421 tggaatcggc gatcatcgcg aggttctcct caagcgaggt ttcgagtacc tcctcgacct
     3481 ggcgatccca tgccttcccc accaacgtga catgcgtcgc accgctctcc agcagcgccc
     3541 gcagattcgc gtcgtctccc actcgagcgc ctgcccggcg tgtacttccg aaggcgacga
     3601 gcgtcgcgtt gcgcagccca atcgcactgg cgcgagcgaa gaactccgcg tccttctggt
     3661 tcgagcctgg gtagcccccc tcgatatagt ggatgccgag gctgtcgagc ttgcgagcga
     3721 tcttgatctt gtcttcgacg gaaagcgaga tgccttccat ccccgcacca tcgcgtaggg
     3781 tcgtgtcgta gagctcaacg ttcatcgtca cggtcgcgca cctctctgga atagcatggt
     3841 gggcagagct tgcacgaccc cgatgaccgc cgtccgcatt gcgcggacgg ccgaaccacc
     3901 tctcacctca ttcagcatcg gtcttgcact tccggcttct gtcgacatca gtcctggagc
     3961 gcggcggcga cgaggtcgcc catctcgctc gtcgcgacca ccgcctcgcc ttcgctcgcg
     4021 atgtccggcg tccttcggcc agaagcgagg gcgcgatcca ccgcatgctc cacggcccgc
     4081 gccgcctcct cctccccgaa cgaaagacgc agcatcatca ccacactcag aatcatggcg
     4141 atggggttcg ctcgtccctg gccggcgatg tccggggcgc tgccgtgaat cggctcgtac
     4201 aggcccatgc ccgtaccatc tcgccgccgg cgccccaggc tcgccgaggg aagcaacccc
     4261 atcgaaccgg cgagaacgct cgcctcgtcc gtcaggatat cgccgaacag attctccgtc
     4321 acgatcacgt cgaaatcccc cgggcggcgg ataaggtgca tcgcacacgc gtcgacaagc
     4381 atgtgttcta gcgccacgtc cggatagtcg cgcgcgacct cgctcgtgac ctcgcgccac
     4441 aagcgtcccg actccataac attcgccttg tcgacgctcg tgaccttcct gcggcgagtc
     4501 cgggccatct cgaaggcgac gcgcacgatg cgggctacct cgcgttcgtt gtagcgcagc
     4561 gtatcgacag cctggcgtcc ggacgcgttc cgccagcgtc gcttcggacg tccgaagtag
     4621 atgccgcccg tcagctcacg gatgacgacg aggtccacgc cctccaccac ctccggcctg
     4681 agcgggccac tcgcgcaaag gaagctctgc gtccgcacgg gacggatgtt cgcaaacagc
     4741 cccaacccct tgcggagacc aaggatcgcg tcctcggggc gtactggcgc cgcgggatca
     4801 tcccacttcg ggccgcccac agcgccgaac aaaacggcct ctgcgcgacg gcatcgctcg
     4861 agggtttcgc tccggagggc cgtaccgtgg gcatcgatac tagcgccgcc caccgcatcc
     4921 tgctcgaact cgaaccgcag gccgaaccgg cgctccacgg cacggaggat ctttatgccc
     4981 tcggcggtaa cctcgggccc gatgccatcg cccgggagga cgacgattct acgcgtcacc
     5041 gaggaggtcc ttccggtacc acgcccatcc cggatgatct ggcgcctcat cgggccggta
     5101 gacatcggtg agcacgtagc cgcgacgctc gtaataccgg cgcagccgat gacgcgaggc
     5161 cagcaccccc agccgcacgg cctttcctcc gtgctcccga gcgcgagcct ctgcttcgcg
     5221 catcagccgc gacccaaccc agccgggggc gtccggcact ataccgaggc ggccgacgta
     5281 ccaatcacca tcctcgagct gcgccagacg caccgagccg accatgacgc catcctgttc
     5341 tgcgagcaag accatccctc gctccagcca accacggata tcgtcctccg tctcatggag
     5401 ggctccgtag tccggcatct cctcgcggta gccctcgaac gccgcacggg tgatggcgag
     5461 cacggcgggg acgtcgcttc cgctcgcctc acggaccgtt acgcccagct gtaccttcac
     5521 catcgccaca ctcaagaccc ggcggtatcg ctccatcgcc gttgccaacg gcgcgttcgg
     5581 acgcgattcc tacgccgtcg ccagctcttg ctcgcggcgt cgctcgtagg cggcgatggc
     5641 gtgctcgtgt tggagcgtga ggccaatgtc gtcgaggcca tgcagcagtc gctcgcggac
     5701 gaacgggtcg atcgcgaacc tcgtgacgac gccgttgtga tcgcggacct cctgccgttc
     5761 gaggtcgacc gtggcttcgt agccggggcg ctcgatcgcg cgagccatga gagtatcgac
     5821 gacgtcctgt gggagttcga gcgggagcat gccgttctga tagcagttgt tgcggaagat
     5881 atccgcaaag cttggtgcga tgatgactcg aataccgaac tgcgagagcg cccacggcgc
     5941 gtgctcgcgc gacgagccgc atccaaagtt gcggccggtc acgaggatct tcgcctcgcg
     6001 atagtagggc tggcacagca cgaagtcggg attgggggag ccatcgggaa ggcgcgccca
     6061 atcgaagaac aggaattcgc cgaagccggt gcgctcgatg cgcttgagaa actgcttcgg
     6121 gatgatctgg tcggtatcga cgtcattgcg atcgagtggc gcgacatatc ccgtgacgac
     6181 cgtgaacggc tccatgctca cgccacctct cgcgccaggt cccactcgcg gatgtcgacg
     6241 aagtggccgg ctatcgccgc tgcggctgcc atctgcggac tgacgaggtg cgtccgtccg
     6301 cccttgccct gccgcccttc gaagttgcgg ttcgaggtgg acgcgcagcg ctcgccctcc
     6361 tggaggacat cggggttcat tcccaggcac atggaacatc ccgcctcgcg ccactcgaac
     6421 ccggcctccc gaaagatgcg gtccagcccc tcgcgctccg cctgagcctt caccagcatc
     6481 gagcccggca cgaccatggc gcgcacgctc ggggccacct tgcggccttt cacgatggcg
     6541 gcggctgctc gcaggtcctc gatgcgcgca ttcgtgcatg agcccagaaa cacgcggtcg
     6601 acggcgaggt cctgcatgcg agtcccgggc cgaaggccca tgtactcgag cgctcgttcc
     6661 gttcccgacg gatccggcgt gtcggccggg tgaggcacgc gaccggtcac aggcacgctc
     6721 tgcgcgggtg tcgtgcccca ggtgacgaat ggctccatcg tgctcgcgtc cagcgccacc
     6781 gtgcgatcga aggttgcgcc tgggtcggtg ggaagcctcc gccagcgctg aaccgcgcgc
     6841 tcaaaggcct ccccctgggg agcatacata cgcccacgaa ggtaatcgaa tgtgacgtca
     6901 tccggggcaa tcatgccggc ccgcgcgccc gcctcgatag acatgttgca caccgtcatg
     6961 cggccttcca tcgagagcga ccggatcgca tcgcccgtgt actcaatgac gtagccggcc
     7021 cctcccgacg tgccgatcgc gcgaatgatg ccaagagcga cgtccttcgc ggagacaccg
     7081 tagggcggca ctccatcaac ccgcacctcc atcgtcttcg gcttggcgac gggcagcgtc
     7141 tgtgttgcca gcacgtgttc gacctcggac gtaccgatcc ccatcgcgaa cgccccgaag
     7201 gcaccatggg tcgaggtgtg gctatcgccg cagacgatgg tcatcccagg ctgagtgcgt
     7261 ccttgctcgg gggcaatgat gtgcacgatg ccctggtact ggctgtggta gtcgaacacc
     7321 gtgatgccga agtcctgcgc gttacgcacg agcacttcga cctgctgccg tgagatgggg
     7381 tcttcgatgg gaagcgaccg atccgtcgtg ggcacgttgt ggtcgacggt cgccagcgta
     7441 aggtccgggc gcctcacgtt gcggccggcc aagcgcaatc cctcgaacgc ctgaggcgag
     7501 gtgacctcat gcacgaggtg gaggtcgatg tacaggagcg taggcctgcc cggctcctcg
     7561 tacaccacgt gagcatccca gatcttgtcg aacagcgtct tcggctgcat gagctcatct
     7621 ccacagataa cgtggtgagg agagcgttgc cgcctcctca ccttatggta acaggtggat
     7681 ggctacctca gaggcgtcgc gcctcggcct gactatgggt tggtccggac ctccgcgtcc
     7741 gtcggacgat gcacggaaag ggctcggttc agggcgttca tgtacgcctt cgccgaagca
     7801 acgatgatat cggtgtccgc gccgcgcccg acgtaggtcc gaccgttcat ctcgatccga
     7861 atcgtcacct cgccctgggc atcgatgcct tctgtcacgc tcttgaccga gaactcggtg
     7921 agccgattgt cgatctgcac gatgcggttg atagccctgt agaccgcatc gacaggcccc
     7981 gtacccaagg ccgcgtcgga taagacgcgc ccatccgggg cgacgaggcg gaccgtcgca
     8041 gtcggcgtcg tatggtcgcc ggccgtcacc tgcacgaggt cgaggcgata cacctcttgc
     8101 gtcgtccgga gcttgtcggc gacgagcgct tcgaggtcgc ggtcgtcgat ctgggccttc
     8161 ttgtcggcca gctccttgaa ggcgaggaaa gcgcgcgaca tctcctcgtc cgtcagttcg
     8221 tatcccagct cctcgagccg ggccttgaag ccgtggcgac ctgacagctt gccgagcacg
     8281 acctcggcgc cgccggaggg ccagccaatc tcgtgggcat ccatgatctc gtaggtttcg
     8341 cgcatcttca gaatgccgtc ttggtggatg ccggactggt gccggaaggc gttggcacca
     8401 acgatggcct tgttcggctg gacgaccatg cccgtcagat cggagacgag gcggctggtg
     8461 cggtagatct cgcgggtctt gaccccaacg tccacgccaa agtagtcgcg gcgcgtcttc
     8521 agcgccatca ccacctcttc gagcgaagcg ttgccgcctc gctcgccgat gccgttgatg
     8581 gtgcattcga tttgtcgcgc tcccgctcga acagcagcga gcgaattcgc cgtcgaaagc
     8641 ccgaggtcgt tgtggcagtg caccgagatg cgcgcgcggt ggatattggg aacgttctcc
     8701 tgaatctccc gaaggaattg ctcgaactcc tccgggatgg cgtaaccaac cgtgtcggga
     8761 atattcacgg tcccggcgcc tgcctcgatg cactcggtca gcatgcgata gacgtactct
     8821 cgctccgagc gcgtcgcatc catggggctg aactcgacat cgtccgtgta cttcttggcc
     8881 cgcgcgacca tggtgcgcgc cagctcgagt acctcgtcgc ggtccttctt cagctggtgc
     8941 atctgatgga tgtcggagct ggacacgaag acatggatgc gaggatgctc ggcatgccgc
     9001 accgcatccc agcacgcatc gatgtcggcc gggtgcgcgc gcgcgaggcc tgcaatggcg
     9061 cagccacgca cctccttggc gatggcggtc accgcctcga agtcgccggg agaggagttc
     9121 ggaaagccag cctcgatgta gtctacgccg agtcgctgga gctgatgcgc gatctccagc
     9181 ttgtcggtcg cgttgagcgc gacacccggc gattgctcgc cgtcacgcag cgtcgtatcg
     9241 aagatgagaa ctcggttgtc ggtcatgact cgctcccttg gggaatcgat gcgcgccatg
     9301 gcgcgcgcct actcgctgct aagccggccc ttccgcctcc tcactaccgc gagaagacga
     9361 agggccgtcg aagagcgagg ccgcagcgac cgccaacccc caaggttatc cgttcaacaa
     9421 aacccatggc ccaaaccgat tgcaccatct gtcacgcctt cagccatgcc atcatggcgc
     9481 gcaactcctg accgatctgc tcgatgggat gttccgcgtc acgacggcgc atggccatga
     9541 acgacggcct gccggcctga ttctccagaa tccattcacg ggcgaaggtg ccgtcctgga
     9601 tacgactgag cacctgctcc atgttgtcgc gaacgtgccc atcgatgacc ttcggaccac
     9661 tcgtgtagtc cccgtcctcc gccgtatcgc tgatggagta gcgcatgtag ctcatgccac
     9721 cctcgtacat caggtcgatg atgagcttga gctcgtgcat gcactcgaag tatgcgagct
     9781 cgggttggta gcctgcggaa accagcgtct cgaacgcgtt cttcacgagc gaggtcaacc
     9841 ctccgcacaa gacagcctgc tcgccaaaga ggtcggtctc cgtctcttcc ttgaacgtcg
     9901 tctcgagccc gcccgctcgg agggccccga tgccctttgc gtaggcgagc gtgagctgac
     9961 gcgcctgctc gctggcgttt tggtggacag cgaagagcgc gggcacgcca ccgccctcca
    10021 cgaacacgcg ccggaccaga tgaccaggcc ccttcggcgc gaccatcgcc acatcgacat
    10081 ccgcgggcgg ctggatctgc ccgtagtgga tgttgaatcc gtgcgcgaac atgagcatct
    10141 tgccactgcg catgtgcggt tcgatgccct tctcgtacag ctcgcgctgc acctgatcgg
    10201 gcgcgaggat catgatgagg tccgacgcct ccgcgacgtc gttgaccgag gccacgcgca
    10261 gcccatcatc ctccgccttc ttccagctct tgctgcccgg gtagagcccg accgcgacat
    10321 cgcagccact gtccttcagg ttcaacgcgt gcgcgtggcc ctgactccca tagccgatga
    10381 cgccgatcct cttgcctttc agcacaccga ggtcagcatc cttctcgtag tacatctgcg
    10441 ccatgagttg gcttccctct tcccgtcagt cgctcacgta cggcaaggcg cccttgggac
    10501 ggcgaccggc gtgggcgtgg aactcacgga cgtcctgcgg ttcaacctcg gtcaccgcgg
    10561 tatcgcctac cgcgccgcgt gttagcgcga tccggcccgt gcggacgacc tcgcgtatgc
    10621 cgtagggctt cactgcctgg agaaacgcgt cgactttgtc ctcgccgccg gcgacctgga
    10681 tgatcaagga tgtctcggcg acatcgacca catcggcgcg gaacacctgc gcgatctgga
    10741 tgatttcgct acggctgcgg gtgttgcagc gcaccttcac gagcgccagc tctcgcgcca
    10801 cgatcgggtc gtgggtgatg tccgccacct tgaccacgtc gatcagcttg tacaactgct
    10861 tttcgacctg ctcgatgacc gtcttcgccc catcgacgac gagggtcata cgggagatgc
    10921 caggcgtctc cgtgtgtccg accgcgaggc tctcgatgtt gaacccccgc cgccggaaca
    10981 ggctagccac gcggttcaga acgcccggct ggtcctcgac gagagccacg atggtatgca
    11041 gcgattgccg cggccgggcg ccgttcctag tgacgtttcg cgtcgtcatg agaccgtcga
    11101 ccccagcaga tcttcgacgc cttcgatcgt ctcgaagagc gaggctcccg cgggcaccat
    11161 aggccacacg ttgccttcct cctcgacgcg gaagtcgatg atgaagggac cctggtatgc
    11221 acgagcctcg tcgatggctg ctctcacgtc gcccttgtcc tccacgcgaa gcgccgggat
    11281 accgtaggct tccgcaagct tcacgaagtc gggctgggcg atgcgcacat cgacgagatt
    11341 gttcttgtaa aacagctcct gccattgacg gaccatgccg aggtagccgt tgttcatgat
    11401 ggcgaacttg atgggctgct tctcgtcgat gatcgtggcg agctcctgca tcgtcatctg
    11461 gaatccgcca tcgccggcga tcgaccacac ttcggcactc gggtcggccg cttgggctcc
    11521 catggctgct ggaacctcga accccatcgt gccgagcccg ccggagctga cgaaccggcg
    11581 cggcttgcgg aactgatagt gctgcgccgc cc
//
LOCUS       DATMBB010000305         4954 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_131974, whole genome shotgun sequence.
ACCESSION   DATMBB010000305 DATMBB010000000
VERSION     DATMBB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131974"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VNL92_06565"
     CDS             <1..507
                     /locus_tag="VNL92_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HWO94411.1"
                     /translation="SVAVIKVGAATEVELKEKKHRVEDALSATRAAVEEGIVPGGGVA
                     FINALPSLDKVQAEGDEATGAAILRRALEEPLRRIAINAGQDGSVIVQRVKGLPVGEG
                     YDALNDEYGDMRKKGIIDPAKVTRSALENAVSIAGMVLTTNCLVSDLPEKETAGAAAG
                     AGAYPDMY"
     gene            581..1006
                     /locus_tag="VNL92_06570"
     CDS             581..1006
                     /locus_tag="VNL92_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94412.1"
                     /translation="MMVDIEEFQKVVDYCDVFAIGFRLFPERLIVDTRANAEQGPMIR
                     IVEPLESVQARFYWLGRERPSFGPPERFMFVPWPHSLQYLEEIGILERVRDRLVQVDP
                     QMAAACDDAFAGLRKLERAGIVEAIRGEERYRTLWSVLP"
     gene            1084..2070
                     /locus_tag="VNL92_06575"
     CDS             1084..2070
                     /locus_tag="VNL92_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWO94413.1"
                     /translation="MPPLYAGIDIGGTKILSIVAQDDGRIIGEQQIATPVNAEPAVVL
                     NDAAACARAAADLAGVPWSDIAAAGIAIAGPADYNRALVTESPNLPAWRNVPAGAIIG
                     ELLGRPALLENDANAAALGEHRFGAGQGTRHMVYLTVSTGIGGGIIIDGRLYRGASGS
                     AGEFGHMVVQPDGARCGCGNHGCLETVASGTAIERDAAQALAEGYSGPLARMLDASRE
                     MRAEFVEAAAKEGDEVAAAIIRTAGERFGVGLAAIVNILNPEVIVVGGGVSNMGERYL
                     GPAEEAMRRHAFDLPARAVELRLAALGKYSAALGAVVLASEGRRQAAPEGSA"
     gene            2111..3448
                     /locus_tag="VNL92_06580"
     CDS             2111..3448
                     /locus_tag="VNL92_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWO94414.1"
                     /translation="MARASHRRILVGLVLLSLGLLVATASCSNAPEGGGVHVLSADGA
                     VGPVMANYLDRGIEQAEEGEAAAVVIELDTPGGLDSAMRDIVKDITNADVPVVVYVAP
                     SGARAASAGTFIVMAAHIAAMAPGTALGAAHPVDVSGGDVEGDLGEKIENDAAALIRS
                     LAEQHGRNADWAERAVRESVSISANEAVDLNVAELVVPSTEQLVDAIDGRSVELHDGR
                     EVRLATAGQPIRENDMTAFEDFLALLSDPNIAFLLLTLGSLALVVELFHPTIFGGVAG
                     ALALILAFFSLGTLPVNWAGVALIALAFALFVLELFVTSYGILGVGGAVAMALGGLLL
                     TTSDQREFEVSVWLAFGLPAVIGALFAAFVLYVFSSRSRPVAIGTEALIGQTAVATSP
                     LAPSGYVLLHGERWRAIAPRTVAEGEAVRVTGLRGLELTVEPTDAAPRAASPE"
     gene            3529..4398
                     /locus_tag="VNL92_06585"
     CDS             3529..4398
                     /locus_tag="VNL92_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWO94415.1"
                     /translation="MEIAIGVAIAVVVVLVIVIAAAVRIVAEYERGVIFRLGRLMGAR
                     GPGLFLIIPIIERMVRVDLRIITMDIPSQEVITRDNVTIRVNAVLYFKIVDANQAVTA
                     VLDYIRATSQIAQTTMRNVVGQSLLDELLSEREDINQRLQRIIDEQTEPWGVKVTIVE
                     IKDVELPTTMQRAMARQAEAERERRSKIIAAEGEKQASETLADAGRVLAAVPTTMQLR
                     FLQTLSEIATEKNSTIVLPVPIDLLTAFIPGVRQAVPPSPSPPASSEIADLQTGMGGI
                     AEDEPPSGPTEAL"
     gene            complement(4453..>4954)
                     /locus_tag="VNL92_06590"
     CDS             complement(4453..>4954)
                     /locus_tag="VNL92_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWO94416.1"
                     /translation="PPTGWLARRRPRRRSDVATIVGAGNITAEMFWRLADEAGDAVIL
                     ADRDGVVRFWNHRAESMFGYASDEALGQSLDLIIPEKYRSRHWEGYHRVMATAQSRYA
                     AGDLLAVPALRKDGSTLSIEFTIAPIKNAGGDIEMVGAIVRDVTERFNRERELRARLA
                     ELERAT"
BASE COUNT          810 a         1643 c         1669 g          832 t
ORIGIN      
        1 agcgtcgcgg tgatcaaggt cggcgccgcc accgaggtcg agctgaagga gaagaagcac
       61 cgcgtcgagg acgcgctttc ggccacacgg gccgccgtgg aggaagggat tgttcccggc
      121 ggtggcgtcg ccttcatcaa cgcgcttccc agcctcgaca aggtgcaagc ggagggcgac
      181 gaggcaacgg gcgcggcgat cctgcgccgg gcccttgagg aaccgctccg acggatcgcg
      241 atcaacgccg gccaggatgg gtcggtcatc gtgcagcggg tcaagggcct gcccgtcggt
      301 gagggctatg acgcgctgaa cgacgagtac ggcgacatgc gcaagaaggg catcatcgac
      361 ccggcaaagg tgacccgttc cgccctcgag aacgctgtca gcattgccgg catggtcctc
      421 accacgaact gtctggtcag cgacctgccc gaaaaggaga cggccggtgc ggcggcgggc
      481 gccggcgcct accccgacat gtactagcga gacgcctcaa ctctaggggg tccccggtcc
      541 gaattggccg gggacccacg caccccgtgg gtggaaaact atgatggtgg acatcgaaga
      601 gttccagaag gttgtcgatt actgcgacgt gttcgctata ggattccgcc tgttccccga
      661 gcgactcatc gtcgatacgc gggcgaatgc cgagcaaggc cccatgatcc gcatcgtcga
      721 gcccttggag tccgttcaag cacgcttcta ctggctcggc cgcgaacgcc cgagcttcgg
      781 ccctcccgaa cgcttcatgt tcgtgccgtg gccgcacagc ctccagtacc tggaggagat
      841 cggcatcctc gaacgcgtcc gcgatcgtct cgttcaggtc gacccccaga tggcggcagc
      901 gtgcgatgac gcctttgcgg gcctccggaa gctcgagcgg gcgggcatcg tcgaggccat
      961 ccggggagag gagcgatacc ggaccctctg gtccgtcctg ccctgaacgt cggctacctc
     1021 cggggcttgc ctcgccgaag atgcgcgttt gacaggcata agcccggctg acatgctcgg
     1081 gtcatgcctc ccctgtacgc cggcatcgac atcggtggaa ccaagatcct cagcatcgtc
     1141 gctcaagacg acgggcgcat catcggcgag cagcagatcg cgacgcccgt caacgcagag
     1201 cctgccgtcg tcctcaacga tgccgccgcc tgcgcgcggg ctgcggctga cctcgcgggc
     1261 gtcccttggt ccgatatcgc tgccgcaggc atcgccatag ccgggcccgc cgactacaac
     1321 cgggccctgg tgaccgagtc gcccaacctg ccggcttggc gcaacgtacc ggcgggcgcc
     1381 atcattggtg agctgctcgg ccgcccggcg cttctcgaga acgacgccaa cgcggccgcc
     1441 ctcggagagc atcgtttcgg ggcgggccaa ggcacgcgcc acatggtgta cctcaccgtc
     1501 agcacaggca ttgggggcgg catcatcatc gatggccgcc tgtaccgggg cgccagcggc
     1561 tccgccggcg agttcgggca catggtcgtg cagcccgatg gagcccgatg cggatgtggc
     1621 aaccacggct gtctggagac ggtggcgagc ggcacggcca tcgaacgcga tgcggcccaa
     1681 gctctcgccg agggctactc cgggccgctc gcccggatgc tcgacgcatc gcgcgaaatg
     1741 cgcgctgagt tcgtggaggc ggcagcgaag gagggcgatg aggttgcggc ggccattatt
     1801 cgaacggcgg gcgagcgctt cggtgtggga ctcgccgcca tcgtgaacat cctgaacccc
     1861 gaagtcatcg tcgtgggcgg cggggtgagc aatatgggcg agcgatatct tggccctgcc
     1921 gaggaagcga tgcggcgcca cgccttcgat ctcccggcgc gggctgtcga gttacgtctc
     1981 gcagccctgg gcaagtattc ggcggcgctt ggggccgtcg ttctggcgag tgagggccgc
     2041 aggcaggccg cgccggaggg ctcggcctga cgtaccggta gccggccccg atagtcccta
     2101 tactgatgcc atggctcgcg catctcatcg tcgcatcctc gttggcctcg ttctcctcag
     2161 cctcggcctg ctcgtggcca cggcgagttg ttcgaatgcc cccgagggtg gcggagtcca
     2221 cgtgctctcc gccgacgggg cggtagggcc ggtaatggcg aactacctcg accgcggtat
     2281 cgagcaggcc gaagaggggg aagctgcagc ggtcgtcatc gagctcgaca cgccgggcgg
     2341 cctcgattcg gcgatgcggg acatcgtcaa ggacatcacg aacgccgacg tccccgtcgt
     2401 cgtctacgtc gctccttccg gcgcgcgggc ggcatccgca ggcacgttca tcgtcatggc
     2461 tgcccatatc gcggcgatgg cgccgggaac ggcgctcggc gcggcgcatc ccgtggacgt
     2521 cagcggcggc gacgtggagg gcgaccttgg cgagaagatc gagaacgatg ccgccgcgct
     2581 cattcgttcg ctggccgagc agcacgggcg taacgccgat tgggccgagc gcgccgtgcg
     2641 cgagagcgtc tccatctcag cgaatgaggc ggtggacctc aacgtagccg agctcgtcgt
     2701 accctcgacg gagcagctcg tcgacgccat cgacggccgc tcggtcgagc tgcacgatgg
     2761 ccgcgaagtg cgcctcgcga cggccggtca gccgattcgt gagaacgaca tgaccgcttt
     2821 cgaggacttc ctggccctgc tgagcgatcc caacatcgcc ttcctcctgc tcacgttggg
     2881 ctctctcgcg ctcgttgtcg agctgttcca cccgacgatc ttcggcggtg tcgcgggcgc
     2941 cctggcgctc atccttgcct tcttctcgct ggggacgctt ccggtgaact gggcgggggt
     3001 ggcgctcatc gcgctcgcct tcgccctctt cgtgctcgag ctcttcgtca cgagctacgg
     3061 aatactcggc gtcgggggcg ccgtcgccat ggcgctgggc gggttgctcc tcactacgag
     3121 cgaccagcgg gaatttgagg tttcggtttg gcttgcattc ggactgccgg cggtcatcgg
     3181 ggccctgttc gcagcgttcg tcctgtacgt gttcagcagt cggtctcgcc cggtggcgat
     3241 cggcacagag gcgcttatcg gccagacggc cgtggcgacc tcgccgcttg cgccgagtgg
     3301 atacgttctg ctccacggcg agcggtggcg ggcaatcgcg ccacgtacgg tcgctgaggg
     3361 cgaggccgtc cgggtgaccg gcctgcgagg gctcgagctt acggtggaac ctaccgacgc
     3421 ggccccgcgg gcggcctcgc ccgagtagca cgatcgcgag acgacgcgct tcccgagcag
     3481 cttacggggc gtgttatcgt gaaccaacct gacggaggag gtgcgcccgt ggaaatcgcc
     3541 atcggagttg cgatagccgt cgtcgtagtc ctcgtgatcg tgatcgcggc ggccgtccgc
     3601 atcgtggcgg agtacgagcg aggcgtcatc ttccggctgg ggaggctcat gggcgccagg
     3661 gggcctggtc tcttcctcat catccccatc atcgagcgaa tggtacgcgt cgacctgcgc
     3721 atcatcacga tggacatccc atcacaagag gtgatcacgc gggacaacgt cacgattcgc
     3781 gtcaacgccg tactctactt caagatcgtc gatgcgaacc aggccgtgac cgccgtgttg
     3841 gactatatcc gcgccacctc gcagattgcc cagacgacga tgcgcaatgt cgtcgggcag
     3901 tctctcctgg atgagctgct ttccgagcgc gaagacatca atcaacggct ccaacgcatt
     3961 atcgatgagc agaccgagcc atggggcgtg aaggtgacga tcgtcgagat taaggacgtg
     4021 gagctgccca cgacgatgca gcgcgccatg gcacgtcagg ccgaggcgga gcgcgagcgg
     4081 cgctccaaga tcatcgccgc ggagggcgag aagcaggcct ccgaaacgct ggccgacgcc
     4141 ggccgcgtcc tggccgccgt ccccacgacg atgcaactgc gctttctcca gacgctctcc
     4201 gaaatcgcga ccgaaaagaa cagcaccatc gtgctgcccg tgcccatcga cttgctgacc
     4261 gcgttcatcc ccggcgttcg ccaggccgtt ccgccgtcgc ccagtccacc cgcgtcttcc
     4321 gagatagccg acctgcagac gggcatgggc gggatcgccg aggacgagcc accatcgggc
     4381 cccaccgagg cgctttgatg gaatgagact gggggcaccc ctacgcgcgc gaaggcgcga
     4441 gggggctgga cgttatgtcg cgcgttccaa ctccgcgagg cgcgcgcgga gctctcgctc
     4501 ccggttgaag cgctcggtaa cgtcgcggac tatcgcgcct accatctcga tgtctccccc
     4561 agcgttcttg atgggcgcaa tcgtgaactc gatggagagc gtgctcccgt ccttgcggag
     4621 ggcgggcaca gcgaggaggt cgcccgccgc atagcgactc tgagcagtgg ccatcacgcg
     4681 atggtaccct tcccaatggc gtgatcgata cttctcggga atgatgaggt cgagcgactg
     4741 cccgagcgcc tcatcgcttg cgtagccgaa catgctctcg gcccggtggt tccagaagcg
     4801 gacgacgccg tcccggtcgg caaggatgac cgcatccccg gcttcatcgg cgagccgcca
     4861 gaacatctcg gccgtgatgt tgcccgcgcc cactatcgtc gcaacgtcac ttcgccggcg
     4921 gggacgacgg cgcgcgagcc atcccgtcgg cgga
//
LOCUS       DATMBB010000306         3269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_250046, whole genome shotgun sequence.
ACCESSION   DATMBB010000306 DATMBB010000000
VERSION     DATMBB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250046"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..106
                     /locus_tag="VNL92_06595"
     tRNA            8..106
                     /locus_tag="VNL92_06595"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Sec,seq:tca)
     gene            complement(164..1216)
                     /gene="plsX"
                     /locus_tag="VNL92_06600"
     CDS             complement(164..1216)
                     /gene="plsX"
                     /locus_tag="VNL92_06600"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWO94417.1"
                     /translation="MKIALDAMGGDDAPAATVAGGVLAAQRFGVDVVLVGQEEPLREE
                     LAKQRQVPAGVSIVPATQIIEMAEHPAQAVRQKRDASMVVGMRLVKQGEAAAFVSAGN
                     TGAAMASALLVLGRIRGVERPAIGALVPLSDGRVTLMLDAGANADVRPSHLVQFAQMG
                     AEYLRAALGVANPRIGLLSNGEEPTKGSTLVQEVHALLTTSGLNFYGNVEGKDLFEGL
                     VDVIVTDGFTGNVALKSVEGVLAMINRELRNALTSHWYYIPGALMVQPALRGMAKKFD
                     YSEHGGAPLLGVNGVVIIAHGRSDSNAIANSIRVAAEAVRSGMQDTLRHAFAGAGESA
                     SDGESEAEVTIAAPEH"
     gene            complement(1216..1995)
                     /locus_tag="VNL92_06605"
     CDS             complement(1216..1995)
                     /locus_tag="VNL92_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWO94418.1"
                     /translation="MTATRADPYAPLAHYYDLEISAFEDDISMYLDLARESPGPVLEL
                     GIGTGRVGLALAEHGYDVTGIDTSEAMLAIAQERAASARRSIELLLADMRDFDLERRF
                     GLVVCALGSFRHLLTEEDRLQTLQRVREHLEPAGRFVLHVPAWHTMSWEPNPPPVLFD
                     WSRTNPETDNVVTKFNVVRPNGAEQLQVLTLMYDEQDSLGSIRRLTVEMPLYTPTRSE
                     LALLFGQAGLRALDWHGGFDFGPYDALSPELIAIAAREEAR"
     gene            complement(1992..>3269)
                     /locus_tag="VNL92_06610"
     CDS             complement(1992..>3269)
                     /locus_tag="VNL92_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWO94419.1"
                     /translation="DVRGSAAPRRSPRHGDLGGLVSDGANGTSHLPAAKATAARIIQE
                     LREPLLALSHYIHKHPEIAFQERESSAAVAGFLAQHGFNVRRPYLELETAFRAEAGHG
                     EPTVAIIAEYDALPGIGHACGHNIIAAAAAGAAIAAARSLQEAGAQGRIIVFGCPAEE
                     VGGGKAILIERGAFSDVHAAMMVHPANRDIALVRSLAMLHLVVRYEGRASHAAAAPSL
                     GRNALDAIVLAYGAIGLLRQQVRETARIHGVITDGGKAANIIPDRTAGSFMIRAEENE
                     YLEELRERVLACFRAGAEATGCRLEHEWAEAPYLALNSNAPLAEAYLANLRTTGREAP
                     QPSGVRAANMGSTDMGNVSWVVPSIHPSLAVAPAGVPIHTPEFAVYAAAAEGDRAVMD
                     GATALALTAIDVLLRPELREAAQESFERSCAAR"
BASE COUNT          514 a         1080 c         1135 g          540 t
ORIGIN      
        1 tgtccgtggg agtggatcgg tcccggtggg tcgcacggtc ttcaaaaccg gttgcggggc
       61 cgcctgcgcg gccctgggtg ggttcgactc ccacgcactc ccgccatccg tcgagcgtcg
      121 ccgcccggaa cgcgtccctc cgtaccggcg agatgacgcg ctcctagtgt tccggcgccg
      181 cgatcgtcac ctcggcctcg ctctctccgt ccgatgcgga ttcgccggcg ccagcgaacg
      241 catggcgaag cgtatcttgc atgccggacc ggacggcctc cgccgccacc cggatcgagt
      301 tggcgatcgc attcgagtcg ctgcgcccgt gggcgatgat gacgacgccg ttgacgccga
      361 gcaggggcgc gccgccgtgc tcgctatagt cgaacttctt cgccatgccc cgcagagcgg
      421 gctggaccat cagggcaccg gggatgtaat accagtgact cgtgagggcg ttccgtagct
      481 cccggttgat catcgccagg acgccctcga ccgacttgag agccacgtta ccggtgaatc
      541 catccgtaac gatgacatcg acgaggcctt cgaacagatc cttcccctcc acgttgccat
      601 agaagttgag gccggacgtg gtgagcagcg catggacttc ctgaacgagc gtgctcccct
      661 tggtcggctc ctcgccgttg ctcaggagcc cgattctcgg gttcgcgacc ccgagcgccg
      721 cacgcaagta ctcggcgccc atctgcgcga attggaccag gtgcgaaggg cggacatcgg
      781 cgttggcgcc tgcatcgagc atcagcgtga cacgcccgtc gctcagcggg acgagcgcgc
      841 cgatggcggg ccgctcgacg ccccggatgc ggccgagcac gaggagcgcc gaggccatcg
      901 ccgcaccggt attgcccgcc gagacgaatg ccgcggcttc gccctgcttc accaggcgca
      961 taccgaccac catggaggca tcgcgctttt ggcgcacggc ttgggccgga tgctccgcca
     1021 tctcgatgat ctgggtcgcg ggcacaatgc tgaccccggc cggcacctgg cgctgctttg
     1081 caagctcctc ccgcaacggc tcttcctgtc cgacgaggac gacgtcgacc ccgaagcgct
     1141 gcgccgcaag aacgccgcct gcaacggtgg ccgcgggagc gtcatcgcct cccatggcat
     1201 cgagcgcgat cttcatcagc gcgcctcctc tctcgcagcg atggcgatga gctccggact
     1261 caacgcgtcg tagggcccga agtcgaatcc gccgtgccag tcgagtgccc gaagtcccgc
     1321 ttgaccgaac agcaacgcga gctcggagcg tgtcggggta tagagcggca tctcgaccgt
     1381 gaggcgtcga atggaaccaa ggctgtcctg ctcgtcgtac atcagcgtca agacctgcag
     1441 ttgctcggcg ccgttcgggc ggacgacgtt gaacttcgtg acgacgttgt ccgtctcggg
     1501 attcgtccgc gaccaatcga agagcacggg aggagggttc ggctcccacg acatggtgtg
     1561 ccaggcgggg acatggagga cgaaccgtcc cgcgggctcg aggtgctcgc gcacacgttg
     1621 gagtgtttgc aggcgatctt cctccgtgag gagatggcga aagctcccca gtgcgcagac
     1681 gacgaggccg aagcgacgct cgaggtcgaa gtcgcgcatg tctgcgagga gcagttcgat
     1741 ggaacgccgc gccgacgccg cgcgttcctg ggctatggcg agcatcgcct ccgatgtatc
     1801 gatgccggtg acgtcgtagc catgctcggc gagggcgagg ccgacgcgac cggtgccgat
     1861 gccgagttca aggaccgggc cgggcgattc gcgggcgagg tcgaggtaca tcgaaatgtc
     1921 gtcctcgaac gcggagatct cgagatcgta gtagtgcgcc agcggcgcgt agggatcggc
     1981 gcgcgtcgcg gtcatcgggc ggcgcagctc cgctcaaagc tttcctgcgc cgcctcacgc
     2041 agttccgggc ggaggagcac gtcgatggcg gtgagggcga gcgctgtcgc gccgtccata
     2101 accgcccggt caccctcggc cgccgcggca tagacggcga actcgggcgt gtggatgggc
     2161 actcccgcgg gtgcgacggc gagggagggg tggatggatg gcaccaccca gctgacattg
     2221 cccatatcgg tgctgcccat gttggcggcc cggaccccgc tgggttgagg cgcctctcgc
     2281 ccggtcgtgc gcagattcgc cagatatgcc tcggcgagcg gtgcgttgga gttcagggcg
     2341 aggtaggggg cctctgccca ctcgtgctcc aggcggcagc ccgtggcttc cgcgccggcc
     2401 cggaagcagg ccaggacgcg ctcgcgcagc tcctcgaggt attcgttctc ctcggcgcga
     2461 atcatgaacg agccggccgt ccgatcgggg atgatgttgg ccgcctttcc gccatccgtg
     2521 atgacgccgt ggatgcgcgc ggtctcccgg acctgctgcc gcagcaggcc gatggctcca
     2581 taggcgagca cgatggcgtc gagggcgttg cggccgaggg acggcgcggc cgccgcgtgc
     2641 gaggcacgcc cctcgtagcg gaccacgaga tggagcatgg caagcgaacg gacgagcgcg
     2701 atatcgcggt tggcgggatg caccatcatc gcggcatgga catcggaaaa tgcgcctcgc
     2761 tcgatgagga tcgccttgcc cccaccgacc tcctcggccg ggcagccgaa cacgatgatg
     2821 cgtccctggg cgcccgcctc ctgcaggctc ctggcggcgg cgatagccgc gccggccgct
     2881 gccgccgcga tgatgttgtg cccgcatgca tggccgatgc ccggaagggc gtcgtattcg
     2941 gcaatgatcg ccaccgtggg ctccccgtgg cctgcctcgg cacgaaacgc ggtttcgagc
     3001 tcgaggtagg gacggcgcac gttgaatccg tgctgggcta ggaagccggc caccgccgcg
     3061 gatgactctc gttcttggaa tgcgatttcg ggatgcttgt gaatgtagtg gctcagcgcg
     3121 agaagcggct cgcgaagctc ctgaatgatg cgggcagcag tggccttcgc cgccggcaga
     3181 tgggaggttc cgttggcgcc gtcactcacg agtccgccca ggtcaccgtg ccgtggagat
     3241 cggcggggtg cggccgagcc gcgaacatc
//
LOCUS       DATMBB010000307         2898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_132159, whole genome shotgun sequence.
ACCESSION   DATMBB010000307 DATMBB010000000
VERSION     DATMBB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132159"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /gene="rlmN"
                     /locus_tag="VNL92_06615"
     CDS             complement(<1..1080)
                     /gene="rlmN"
                     /locus_tag="VNL92_06615"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWO94420.1"
                     /translation="MHQSSFFDLSLADLTGRLAEWGEPAYRARQAWANVYRRLVSRYD
                     EMTDLPAGLRSRLAAELPLAMPEALHEVRSDDGLTHKALLRLGDGRTIEAVLMRYERD
                     DDGRARNTVCVSSQVGCAMACGFCATGQGGIERNLTPGEIVGQVVHFARMLRGEEARV
                     SNVVFMGMGEPLANYASVWRAVETLHSAEGLDIGARHITISTVGIIPGIRRLAREALP
                     VTLAVSLHAPDDALRQLLIPTQRRPIAELLQACRDYFKATGRRPSFEYCLIAGVNDSD
                     AQALSLARLLRNAGFPAHVNLIPLNPTPDEAYRRPARARVLSFQRVLADAGINCTVRA
                     EKGVEIAAACGQLRGPRLQGSLDVAL"
     gene            complement(1177..2040)
                     /locus_tag="VNL92_06620"
                     /pseudo
     CDS             complement(1177..2040)
                     /locus_tag="VNL92_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874831.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
     gene            complement(2143..2691)
                     /locus_tag="VNL92_06625"
     CDS             complement(2143..2691)
                     /locus_tag="VNL92_06625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWO94421.1"
                     /translation="MLEGRLVRLRAHRPSDLDACLRWMNDVEVLRTSAGRYPVSPSAE
                     AAWLERVSAGDASGVPRDVHFGIDALSDGRYIGNCSIERVSFENRSAEVGIMIGEKDC
                     WDRGYGTDAMRTMLRFAFREMNLNRIQLDVFDFNARAISSYRKCGFVEEGRRRKAWFA
                     EGSYHDIVVMGVLRDEFEAAPE"
BASE COUNT          529 a          954 c          911 g          504 t
ORIGIN      
        1 gagcgccacg tccagtgacc cctggagccg ggggccccga agctgaccgc atgcggcagc
       61 gatctctacg cccttctcgg ctcgcacggt gcagttgata ccagcatcgg cgagcacgcg
      121 ctggaacgag agcacgcgag cccgggcggg acgacggtac gcctcgtccg gggtggggtt
      181 gagcgggatc aggttgacgt gcgcggggaa tcccgcgttt cgcagcagcc gggcgagtga
      241 gagggcttga gcgtcgctat cgttcacccc ggcgatgagg cagtactcga agcttgggcg
      301 tcgcccggtc gctttgaagt agtcgcggca tgcttggagc aactctgcaa taggccggcg
      361 ctgcgtgggt atgaggagct gccgcagcgc gtcgtctggc gcgtggagcg agaccgcgag
      421 cgtgaccggg agcgcctcgc gcgccagcct ccgtatgccc ggaatgatgc ccaccgtgct
      481 gatcgtgatg tggcgagcac cgatatcgag accctcggca gaatgcagcg tctctacagc
      541 gcgccacacg gaagcgtagt tggccagtgg ctcgcccatg cccatgaaca cgacgttgga
      601 gacacgggcc tcctctccgc gcagcatgcg agcaaagtga acgacctggc cgacaatctc
      661 gcccggcgtg aggttccgct cgatgccacc ctgccccgtc gcgcagaacc cacacgccat
      721 cgcgcaacct acctgactcg agacgcacac cgtattgcgc gcccggccat cgtcgtcgcg
      781 ttcgtagcgc atcagaacgg cctcgatggt tcggccatca ccgagccgaa ggagtgcctt
      841 gtgcgtcagg ccatcgtcgg agcgcacctc atgcagcgcc tccggcatcg cgagcggcag
      901 ctccgcggcg aggcggcttc ggagcccggc aggcaggtcc gtcatctcgt catagcgcga
      961 gacgagccgc cggtagacgt tcgcccacgc ctgccgcgcg cgatatgcgg gttcgcccca
     1021 ctcggcgagg cgcccggtca gatctgccag gctaaggtcg aagaacgagg actggtgcat
     1081 cttgccttcc aatgtacggg gtaacggccg ccgagcaaag acaagaacgg agatatcgat
     1141 ctccgttctg cgaactcgcg ccgattgtga cgaaccttag cgaagatgct gggccggatt
     1201 ctccgccatc tgccgcaggc gctcgacgcg ctcggcgacg ggcgggtgcg tgctgaacag
     1261 gccagccaag tcgcgtccct tgagtgggtt gacgatgtag agcgccgccg tgccctcgct
     1321 gaccttcata ggaatctgtt gggcccccat ctcgatcttc tggagggcgc tcgccagggc
     1381 ctcgggccgc ccgctgatca tcgcgcccgt ccgatcggcc tcgtactcgc gcgatcgcga
     1441 gatgccgaac tggatcatcg ccgctgcgat gggggcgacg atgatcatca gcaagccgac
     1501 cagcatgccg ccgttgtcgc gacggcctcc cccgaagatg agcgcccatt gcgcccacca
     1561 cgtcagcatc gtgatggccc ccgcgagcgt cgcgacgata gtgctcgtga gcgtatcgcg
     1621 gttgcgcacg tggctaacct cgtgggcgag cacgccgaac agctcgtcgc ggttcaggat
     1681 gcggaggatg cccgtcgtca cagcgatcgc cgcgtggtcc gggctgcgtc cggtggcgaa
     1741 cgcattcggc tggtccgcct cgatgatgtg gacgcgcggc ttcggcatgc gcgcgtacat
     1801 gacgagctcg tcgacgagcg cgtgcaacta aggcgcctcc tccggcgtaa cctcacgcgc
     1861 gcgagacatc ttcagcatca ggctatcgga gaaccagtag gagatgaagt tcatgacgag
     1921 ggcgaacatc aggcccatca ccatgccggc cgtgccggcc aacacgaacc caacgaagat
     1981 gaacagggcg gtgagcgccg caagaaggat cgtcgttctc agcgtgtttc cactgaccat
     2041 cgtgccgttc ttccccttgt ggccgccgcg gccaccgatc gtactgtaca aaaccttgcg
     2101 aaattcgtat tcatcgtagc actcgccgtt tcgtccggca agctattccg gcgccgcctc
     2161 gaactcgtcc cgaagcacgc ccatgacgac gatgtcatga tacgagcctt cggcaaacca
     2221 cgcctttcgc cgccgccctt cctcgacgaa tccgcacttc cggtagctgc tgatcgcgcg
     2281 ggcattgaag tcgaatacat cgagttggat tcgattcagg ttcatctcgc gaaacgcgaa
     2341 ccgtaacatc gttcgcatcg catccgtccc gtaccctcgg tcccagcagt ccttctcgcc
     2401 gatcatgatg cccacttcgg cggaccggtt ttcgaacgag acgcgttcga tgctgcaatt
     2461 gccgatgtac cggccgtcgc tgagcgcgtc gatgccgaag tgcacgtcgc gcggcacgcc
     2521 ggaggcgtcg cccgcggaga cccgctccag ccaagccgcc tctgccgagg gcgagacggg
     2581 gtagcgtccg gccgacgttc gcagaacctc gacgtcgttc atccagcgaa ggcaggcgtc
     2641 gagatcggac ggacggtggg cgcgcaaacg taccagacgg ccttcgagca tcaagttccc
     2701 cctcggacga gcgcggtatc gcggataggc cattattgat caggcagccc aggacatgag
     2761 ccatgataac gttacgctca tagttcctca ctacatccgt tgacatcaac ctaccttgct
     2821 ggctatactt ctctcagaac tgaaggtcag cggcgtatgg caagagacga cttctatcaa
     2881 ttgctcggcg tgaagcgc
//
LOCUS       DATMBB010000308         5340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_36722, whole genome shotgun sequence.
ACCESSION   DATMBB010000308 DATMBB010000000
VERSION     DATMBB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36722"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VNL92_06630"
     CDS             complement(<1..478)
                     /locus_tag="VNL92_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94422.1"
                     /translation="MIRRIRLLRNIGQFDSVDTAAAIALGRLVLVYAENGRGKTTLAA
                     VLRSLGTGNPIPITERRRLAAAHPPHVVLDCEGGPPDAVFQDGRWSRTLPHLAIFDDV
                     FVDANVHSGLVVDARHRQNLHELVLGARGVELSRRIQELVERTEEHNRALRERAAAI"
     gene            complement(475..1854)
                     /locus_tag="VNL92_06635"
     CDS             complement(475..1854)
                     /locus_tag="VNL92_06635"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HWO94423.1"
                     /translation="MAGDSTLNLKSLHLPKLSRELPQEWCWSRLDDLCVGIYDCPHST
                     PQLVDRGPLIARSQDIITGVFRSKQAAQVSEETYRERTLKAVPTHGDLLYSREGTYFG
                     IAAEVPEHTRVCLGQRMVLIRPRTDILNFRYLRYWLNSPVVAAHVRGFRDGTVAERLN
                     LPTIRALPVALPPLTEQRAIAHILGTLDDKIELNRRMSETLEAMARALFKSWFVDFEP
                     VRAKLEGRWRRGESRPGLRAHLYDLFPDRLVASELGEIPEGWEVGTLADVASLNPEVW
                     SKQTRPPKINYIDLSNTKWGRIEAVTVHDAADAPSRAQRVLRPGDTIVGTVRPGNGSY
                     AFISDDGLTGSTGFAVLRPVTTEHAEFVYLAATTSDNIDALAHLADGGAYPAVRPEVV
                     AATPIVRPGYDVLVEFSRTVRSLLTRIALHECESRTLAALRDALLPKLISGQLRVKDP
                     TCILGTADR"
     gene            complement(1844..3349)
                     /locus_tag="VNL92_06640"
     CDS             complement(1844..3349)
                     /locus_tag="VNL92_06640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HWO94424.1"
                     /translation="MADALRGSMDAAEYKHVVLGLIFLKYISDAFEEHHARLEAERAL
                     GADPEDPDEYRAQNIFWVPPEARWAHLKAQARQPTIGQLVDDAMAGIERDNPALKGVL
                     PKEYARPALDKQRLGRLIDLVSNIRVGNAEARSKDVLGRVYEYFLNQFASAEGRKGGE
                     FYTPRCVVTLLVEMLEPYKGRVYDPCCGSSGMFVQSIEFIRAHANGNGNGGRARADLS
                     IYGQESNYTTWRLAKMNLAIRGIDGQIAHGDTFHTDRFPDLKADYILANPPFNVSDWG
                     GERLRDDRRWQYGVPPAGNANFAWVQHIIHHLAPSGTAGFVLANGSMSSNQSGEGDIR
                     KAIVEADLVDCMIALPGQLFYSTQIPACLWFLARDKQNGRFRDRRGHVLFIDARKMGR
                     MVDRTHRELTDEDIARIASAYHAWRGEKEAGEYADVPGFCKSATLEEIRTHGHVLTPG
                     RYVGAAAQEDDGEPFEEKMKRLAAQWREQQAEAARLDAAIAANLKELGYGG"
     gene            complement(3594..3893)
                     /locus_tag="VNL92_06645"
     CDS             complement(3594..3893)
                     /locus_tag="VNL92_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94425.1"
                     /translation="MLYERIRHGLAVRHVEKGELGWRITDARRLTGRITWDPDRSGEV
                     PLLVIDGRPFTWDQVGRMLMAFEGFTLRAVVDDTIEVVGGPLLDKAEEQPKRDKE"
     gene            complement(4243..4368)
                     /locus_tag="VNL92_06650"
     CDS             complement(4243..4368)
                     /locus_tag="VNL92_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94426.1"
                     /translation="MLIHGLQGFHGRSLQLPMREAWRPDRHLLEERYARFRRLAG"
     gene            complement(4405..5169)
                     /locus_tag="VNL92_06655"
     CDS             complement(4405..5169)
                     /locus_tag="VNL92_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWO94427.1"
                     /translation="MPDDTQVRLAAFRFLEEQQRLAGEEGVLPRRVLVEGFTYEGQRV
                     PLMGPQGIFKPRVLREIPLSITTVAVVEGETRPYDDAFGEDGLLRYRYRGADPRHHEN
                     VGLRLAMQRRVPLIYFHGIVPGLYVAEWPVYIVGDDPERLTFTVSVDDRRFAGLGSVD
                     APDIPETEIRRRYATRLFQQRLHQREFRERVVRAYQYHCAVCRLRRDELLDAAHIVPD
                     ADPRGVPSVTNGLALCTLHHAAFDRNVIGITPDYVV"
BASE COUNT          888 a         1768 c         1724 g          960 t
ORIGIN      
        1 tgatcgcggc cgccctctcc cgtaatgccc tgttgtgttc ctctgtccgc tcgaccaatt
       61 cctgaatacg gcggctgagc tcgacgccac gagcgccgag gacaagctca tggaggttct
      121 gccgatgacg agcatcgacc accaagcctg agtggacgtt ggcgtcaacg aacacgtcgt
      181 cgaatatcgc caggtgcggc agcgtgcggc tccagcgccc atcctggaag acagcatcag
      241 gaggtccgcc ttcgcagtcc aagacaacat ggggcggatg cgctgctgct agtcgccgtc
      301 gttcggttat cggaatcgga ttcccggttc ccaatgagcg tagcaccgcg gcaagtgtcg
      361 tcttcccgcg cccgttttcc gcgtagacca gcaccaagcg gccgagcgcg atcgcggcag
      421 cagtatccac ggagtcgaac tggccaatgt tccggagcaa acgaatgcgg cggatcatct
      481 gtcggccgta cctaggatgc aggtgggatc cttgacgcga agctgacccg aaatcagttt
      541 gggcagcagc gcgtcgcgca gggcggcgag cgtgcgggat tcacactcgt gcagcgcaat
      601 tcttgtgagc agagaacgga cagttcgaga gaattcgacc aacacgtcat aacctgggcg
      661 aacgattggc gtagcagcga cgacctccgg ccgaacagca ggatacgcac caccatccgc
      721 aagatgtgca agagcgtcga tgttatctga cgtggtggcg gcgaggtaga cgaactccgc
      781 gtgctctgtc gtgacaggtc gaaggacagc aaaccccgtg cttccagtca gtccatcatc
      841 ggagatgaat gcgtacgagc cgtttcccgg ccgaacggtg ccgacgatcg tgtcccccgg
      901 tcgaagtacc ctctgtgctc ggctcggcgc atcggccgca tcatgcacgg tgaccgcttc
      961 gatgcgaccc catttcgtgt tagagaggtc gatgtaattg atcttcgggg gacgagtttg
     1021 ctttgaccag acctccgggt tcaggctcgc aacatctgcg agcgtcccca cttcccaccc
     1081 ctccggaatc tcgccgagtt cggacgcgac gaggcggtcg ggaaagaggt cgtagagatg
     1141 ggcgcggagg cccgggcgcg actcgccgcg gcgccagcgg ccttccagct tggcgcgcac
     1201 gggctcgaag tccacgaacc acgacttgaa gagcgcccgc gccatcgcct ccagcgtctc
     1261 gctcatccgc cggttcagct cgatcttgtc gtccagcgtg ccgaggatgt gggcgatggc
     1321 gcgctgttcg gtgaggggcg ggagagcgac gggcaacgcg cgtatggtgg gtagattcaa
     1381 gcgttcagcg acggttccat cgcgaaagcc gcgcacatgc gctgcaacaa cgggtgagtt
     1441 cagccagtaa cggagatatc gaaagttgag aatgtcggta cgcgggcgaa tcagtaccat
     1501 cctttgaccc aggcagacgc gcgtatgctc agggacttca gcggcaatac cgaagtacgt
     1561 gccttctctg ctgtacaaga ggtcgccgtg ggtggggacc gcctttaacg tgcgctcccg
     1621 atatgtctct tcggagacct gagcggcctg tttcgaccga aatacgccgg tgatgatgtc
     1681 ctggctccga gcgatcagcg gcccccggtc gacgagttga ggcgtcgaat gagggcagtc
     1741 gtagatgccg acgcataagt catcaaggcg gctccagcac cattcttggg ggagctctct
     1801 ggacagcttt gggagatgta aggacttgag gttgagggtg ctgtcacccg ccatacccca
     1861 gctccttcag gttggcggcg atcgcggcat cgagcctcgc cgcctcggcc tgctgctctc
     1921 gccactgcgc ggcaagccgc ttcatcttct cctcgaacgg ctccccgtcg tcctcctgcg
     1981 ccgcggcgcc gacgtagcgg ccgggtgtga gcacgtgccc gtgcgtgcgg atctcctcca
     2041 gcgtcgcgct cttgcagaac cccggaacat cggcgtattc gcccgcttcc ttctcgcctc
     2101 gccaggcgtg gtacgcgctg gcgatacgcg cgatgtcctc gtccgtcagc tcgcggtgcg
     2161 tgcggtccac catccgcccc atcttccgcg cgtcgatgaa gagcacgtgg ccgcggcgat
     2221 ccctgaaccg gccgttctgc ttgtcgcgcg ccaggaacca caggcacgcc gggatctgcg
     2281 tcgagtagaa cagctgaccg ggcagcgcga tcatgcagtc gacgaggtcg gcctccacga
     2341 tcgccttgcg gatgtcccct tcccccgact ggttcgacga catcgagccg ttcgccagga
     2401 cgaagccggc cgtcccggaa ggcgcgaggt gatggatgat gtgctgcacc caggcgaagt
     2461 tcgcgttgcc ggccggcggc acgccgtact gccaccgcct gtcgtcgcgc agccgctcgc
     2521 cgccccagtc ggagacgttg aacggcgggt tcgccaggat gtagtcggcc ttcagatcgg
     2581 gaaagcgatc ggtgtggaac gtgtccccgt gcgcgatctg gccatcgatg ccgcggatcg
     2641 ccaggttcat cttcgccagg cgccacgtcg tgtagttcga ctcctgcccg tagatcgaga
     2701 ggtcggccct ggcgcgcccg ccgttcccgt tgccgttggc gtgggcgcgg atgaactcga
     2761 ttgactggac gaacattccc gacgagccgc agcaggggtc gtagacgcgg cccttgtacg
     2821 gctcgagcat ctcgaccagc agcgtgacga cacagcgggg cgtgtagaac tcgccgccct
     2881 tccttccctc ggcgctcgcg aattgattca ggaagtactc gtagacgcgg ccgagcacgt
     2941 ccttcgaccg cgcctccgca ttgccgaccc tgatgttgct gacgagatcg atcagccggc
     3001 caagacgctg cttgtcgagc gcggggcggg cgtactcctt gggaagcacg cctttcaacg
     3061 ccgggttgtc ccgctcgatg cccgccatcg catcgtcgac cagctggccg atggtgggct
     3121 gccgggcctg ggctttcagg tgcgcccagc gcgcctccgg gggcacccag aagatgttct
     3181 gcgcccggta ctcgtcggga tcctcgggat ccgcgcctag cgcgcgctcg gcctccagcc
     3241 tggcgtggtg ctcctcgaaa gcgtccgaga tgtacttcag gaagatcagt ccaaggacga
     3301 cgtgcttgta ctcggcggcg tccatgctgc cgcggagggc gtccgccatg cgccagagtt
     3361 cggcttcgta gccgaccgtc gccccgttcc tcgtggtgtc cgccttgccc ttgcctctgg
     3421 gcactccgtt cctcctcgct gcttgctttg aacggctgag ccgtctcgat tatgcgtacc
     3481 ttgtggcctc cggccaaacc ggtgggcctc ggaagatggt caccccgacg ggtgcccgtg
     3541 gcctcttgga cacccgtcca aattctcaga tgcgattgtg aagcacgcgt cggctattcc
     3601 ttgtcccgtt tcggctgctc ctcggccttg tccaggagcg gcccgccgac cacttcgatc
     3661 gtgtcgtcga cgacggcgcg cagcgtgaac ccctcgaacg ccatcagcat ccgccccacc
     3721 tgatcccagg tgaagggccg cccatcgatg acgagcagcg ggacttctcc cgaccggtcg
     3781 ggatcccagg tgatacggcc ggtcagccgg cgcgcgtcgg taatccgcca gccaagctct
     3841 cccttctcca cgtggcgaac ggcgagcccg tgccggatcc gctcgtacag cacctggaac
     3901 aggtcccaga catcggcctc gaaatccccg aggaccgaga actcgtatcc ttcgcgtcct
     3961 tcggccaggc gctcacgcgc atacagggca ctccggtggg cacgagcctc gaccggaact
     4021 cgaacgtgtg ctcgacgccg tcggcatcgg cgaccgtgac cggctgcagc cgcgtgtggt
     4081 cgaactcgac gcccatcagc gcggcggttt cctctttgaa gcaggggtag cagcgtcggc
     4141 cggcgttggc gacgggaatg ctctcgtgtg ctttcagcgg ctgcccacag cgctcgcaga
     4201 cgagtcgttt cggcatgatg tccctcgaac tcgacagtgt gttcagcccg ccaagcgacg
     4261 gaagcgcgcg tagcgctcct cgagcaggtg ccggtcgggg cgccaggcct ctcgcatcgg
     4321 cagttgcaat gaccggccgt ggaacccctg gaggccgtga atcagcatcg gcccgtcctc
     4381 ctcgtcgagc acgtcgcggc gaacctagac gacgtagtcg ggcgtgatcc cgatgacgtt
     4441 ccggtcgaag gccgcatggt gaagcgtgca gagcgccaac ccgttcgtca ctgacggcac
     4501 gcctcgcgga tccgcgtccg gcacgatgtg ggccgcgtcg aggagctcgt ctcgccgcag
     4561 acggcatacc gcgcagtggt actggtacgc gcggacgacg cgctcgcgga actcgcgctg
     4621 gtgcaggcgc tgctggaaga gccgcgtcgc gtagcggcga cggatctccg tctcggggat
     4681 gtcgggcgcg tccacggacc cgaggccggc aaaccgccgg tcgtccacgc tcacggtaaa
     4741 cgtcaggcgt tcgggatcgt cgcccacgat gtagacaggc cactcggcga cgtacaggcc
     4801 gggaacgatg ccgtggaagt agatgagcgg gacgcggcgc tgcatcgcca gtcgaagtcc
     4861 gacgttttcg tggtgccgcg gatcggcgcc acgatatcgg tatcgcagca gcccgtcttc
     4921 gccgaaggca tcgtcgtacg ggcgcgtctc gccttccacg accgccacgg tcgtgatgct
     4981 gagcgggatc tcgcgcagga cgcgcggctt gaagatgccc tgcggcccca tcagcggcac
     5041 acgctgtccc tcgtaggtga atccctcgac gagcaccctg cgaggcagga cgccctcctc
     5101 gccggcgaga cgctgctgct cctcgaggaa tcggaacgcc gccagtcgga cctgggtgtc
     5161 atcaggcatc gagcggcggc cctatgctaa cgagcggcgc ggtccacgct cggaacgctg
     5221 tttccttgcg gcaatcacct cccgctgaac agcaccccac ggcagcctcg gccgggcgcc
     5281 gccgcttgca gaggccttcg ccgagcggcc tggcgtcttt ggacctgtca gcttgaaaag
//
LOCUS       DATMBB010000309         4471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_255425, whole genome shotgun sequence.
ACCESSION   DATMBB010000309 DATMBB010000000
VERSION     DATMBB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255425"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2506
                     /locus_tag="VNL92_06660"
     CDS             <1..2506
                     /locus_tag="VNL92_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWO94428.1"
                     /translation="SQARRGAAKAAGPRFGEAFLELLRPDFIGLALILIATLAVTWLA
                     PLDSAAPKLRDDLVETLGLGVFIAIALALAAGWALYRRMLPGSWPARRRWLGIAASAL
                     FAIGALSLYEPDLTIGAISLSDESAGGRVGRSLTDGPLAILAWLGIGFAAVTLLWPRS
                     VWQLLRRSPQLGRFIVSLRIPQRAWLAAQALWRWLKWLYRSSSDEDTDPLDRIGARNP
                     FDERYEAEPFFLDAEAEHSAFSPGGALATRYSAATTASVGVEATPALESAMPPHAVPL
                     GRTLPLPGFTLPERPAVREVPSSGPGPAPASVIDRAPTATPARWRAAPLDLLKPASER
                     DGQALDNQRRAELIVQTLASFGVDARVVGIQEGPTVTQFGVEPGWEVKTRTVQERDKD
                     GKPVLDKDGRPKMRTEEVSRTRVRVSQITSLANDLALALAAPSIRIEAPVPGRPVVGI
                     EVPNHDATVVNLRSVLATPAFQRVSARSKLALALGQGVSGEPIVTDLAKMPHLLIAGA
                     TGSGKSVCINAIIASLLMRSSPDEVRFVMIDPKRVELAPFAQIPHLAFSRVIVDIDEV
                     PGALQAVISEMEARYRKFAALAVRNLDAYNKHPKVVDRLPYWVVVIDELADLMMSAAY
                     EVERQICRLAQLARATGIHLIVATQRPSVDVVTGLIKANFPSRIAFAVSSQVDSRTIL
                     DMSGAEKLLGRGDMLFLPNDAGQPKRVQGAYVSDQEIETLVEYWRSQAADARIERYDH
                     LLEEARRLHEEEEIEEARDALFERARDLALEHTRISTSMLQRRLRIGYPRAARIMDEL
                     EAEGIVGPAEGGGSREVLVSPGEVDDETDWATIDPA"
     gene            2615..3568
                     /locus_tag="VNL92_06665"
     CDS             2615..3568
                     /locus_tag="VNL92_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94429.1"
                     /translation="MQRYLLRRILLLVPTLLGVSIIVFALVRFVPGDTVAIIAGERGF
                     SSPEVEEAVRRDLGLDEPAWQQYFDWLGGVLRGDLGESLIFDGVTVNDELGDRLPVTI
                     QMALMAMVIGLLVAIPVGIISAIRQDTILDYATRSVGIGFIAIPTFWLGTLVMVIPPA
                     LWGWAPPARYFDLWDRPLDNLELMIIPSALLGVALSGTVMRLVRAQMLEVMRQDYIRT
                     AWSKGLRERTVITRHAIRNAFIPVITLIGLQVPVLIGGTVVLENIFSVPGVGRYLVFA
                     IQQHDYPVVQGVNLLIALIVVVTNLVVDMTYGILDPRVRYS"
     gene            3577..>4471
                     /locus_tag="VNL92_06670"
     CDS             3577..>4471
                     /locus_tag="VNL92_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94430.1"
                     /translation="MNRTDAAPRTEATPGLGTPQRASRAVRWLRWLWWFIRKKPLGAF
                     GALLILILLATAAFAPLLAPQHYSDQVLSEALMGPSRDHLLGTDWVGRDLLSRIIYGA
                     RVSIVVGFGAVAISTVLSTVLGVVTGYYGGLVDTLIQRVVDMWIAFPAIVLLISIIAV
                     FGTGVSQVTIALGILLAAGSTRVVRSAVIAIKNNQYIESARALGAGDLRILTWYVVPN
                     IMATIIVLATVQLGTAILAEATLSFLGYGVPPPQPSWGQMLSLSSLQFMRDVPGLAIF
                     PGVAIALAVYGFNMLGDALRDV"
BASE COUNT          674 a         1567 c         1394 g          836 t
ORIGIN      
        1 ttcccaggcc cgccgtggtg cggccaaggc cgccggtccc cgcttcggcg aggccttcct
       61 ggagttgctg cggcccgact tcatcgggct ggcgctcatc ctcatcgcca cgctagcggt
      121 gacatggctc gcccccctcg acagtgcggc acccaagctc cgcgacgacc ttgtcgagac
      181 gctcgggcta ggcgtcttca tcgccatcgc gctcgcgctg gccgctggct gggcgctgta
      241 ccggcgcatg ttgccgggga gttggcccgc ccgccgccgc tggcttggga tcgccgcctc
      301 cgccctcttc gccatcggcg ccctgtcgct ctacgagccc gacctcacga tcggcgccat
      361 ctccttgtcc gacgagagcg ccggcggccg cgtcggccgc tccctcaccg acggtcctct
      421 cgccatcctg gcttggctag gtatcggttt cgccgcggtg acgctcctgt ggccgcgcag
      481 cgtctggcag ctcttgcggc gttcccctca actcggccgg ttcattgtca gcctgcgcat
      541 tccccagcgg gcatggctcg cggcgcaggc tctgtggcgt tggctgaagt ggctgtaccg
      601 ctcgagctcc gacgaggaca ccgacccgct cgatcgcatc ggggcgcgaa atccgttcga
      661 cgagcgatac gaggccgagc ctttctttct cgacgccgag gccgagcact ccgcattctc
      721 gccgggcggc gctctggcta cccgatacag cgctgcgacc acagcctctg tcggagtcga
      781 ggcgacgcct gccctcgagt ccgccatgcc gccacatgcc gtgcccttgg ggcgaaccct
      841 tccgctgcct ggcttcacgc tcccggaacg ccccgcagtc cgagaagtgc catcgagcgg
      901 ccccggcccg gcgccggcat ccgtgatcga ccgggctccg accgctaccc cggcgcgttg
      961 gcgagcggcg ccgcttgacc tcctcaagcc tgcctccgag cgcgacggtc aagccctcga
     1021 taaccagcgc cgcgccgagc tcatcgttca gacgctggcg agcttcggcg tcgacgctcg
     1081 ggtcgtcggc attcaggagg gcccgaccgt cacccagttc ggtgtggaac cgggctggga
     1141 ggtgaagacg cgtacggtgc aggagcgtga caaagacggt aagccggtgc tcgacaagga
     1201 cggtcggccc aagatgcgaa ccgaagaggt gtcgcgcacg cgcgtccgcg tcagccagat
     1261 cacatccctg gcgaacgacc tcgcgctggc gctcgctgcg ccttcgatcc ggatcgaggc
     1321 accggtcccc ggccgtccgg tggtcggcat cgaggttccg aatcacgacg cgacggtcgt
     1381 caacctccga tcggtgctcg ccacccccgc gttccaacga gtctcggccc ggagcaagct
     1441 tgccctcgcg ctcggccaag gggtgtccgg cgagcccatc gtcaccgacc tcgcaaagat
     1501 gccccacctc ctcatcgcgg gcgcgacggg gtccggcaag agcgtctgca tcaacgccat
     1561 catcgccagc ctcctgatgc gctcctcacc cgacgaggtt cggttcgtca tgatcgatcc
     1621 gaaacgcgtc gagctcgcgc ccttcgcgca gatcccgcac ctcgcgttct cgcgagtcat
     1681 cgtcgacatc gacgaagtcc ccggcgcact gcaggccgtg attagcgaga tggaggcgcg
     1741 gtatcggaag ttcgccgcgc ttgccgtccg caatctcgat gcctacaaca agcatccgaa
     1801 ggtcgtcgat aggctgccct actgggtcgt cgtcatcgat gagctggccg acctcatgat
     1861 gtccgcagca tatgaggtgg agcgccagat ctgccggctg gcccagctcg cccgggcgac
     1921 gggcattcac ctcatcgtgg cgacccaacg cccctcggtt gatgtcgtaa cgggcctcat
     1981 caaggcgaac ttcccctccc gcatcgcctt cgcggtgagc tcgcaggtcg actcgcggac
     2041 gatcctcgat atgagcgggg cggagaagct gctggggcgc ggtgacatgc tattcctccc
     2101 caacgacgct ggccagccga agcgcgtgca aggcgcgtac gtatccgacc aggagatcga
     2161 gacgcttgtc gaatactggc gctcccaggc cgcggacgcg cgaattgaac gctacgacca
     2221 cctcctcgag gaggcgcggc gcctgcacga ggaagaagag atcgaggagg ctcgggacgc
     2281 gctcttcgaa cgtgcccgcg acctcgcgct cgaacacacc cgcatctcga catcgatgct
     2341 gcagcggcgc ctccggatcg gctatccgcg tgcggcccgc atcatggacg agctcgaggc
     2401 cgagggtatc gtcggtcccg ccgagggagg cggctcacgc gaggtgctcg tctcgccggg
     2461 cgaggtcgac gacgagacgg attgggcaac aatcgacccc gcctgaggcc gcacgcgcgt
     2521 ggatcggact ttgttccgtt tgtcacgttt tcatcattgc gcgcgcgtgg tacgctaagc
     2581 tctctcacgt atcccggtcc cgctcgaggt cttgatgcag cggtatctgc ttcgccgaat
     2641 cctcctgctt gtcccgaccc ttctcggggt cagcatcatc gtcttcgctc tcgtgcgctt
     2701 cgttccaggc gatacggtcg cgatcatcgc gggcgagcgc ggcttttcca gtcccgaggt
     2761 ggaggaagca gtccggcgcg atctcggcct cgatgagccg gcctggcaac agtacttcga
     2821 ttggctggga ggcgtcctgc gcggcgacct cggcgaatct ctcatcttcg acggcgtcac
     2881 cgtcaacgac gaacttggcg accggctccc cgtcaccatc cagatggcac tcatggcgat
     2941 ggtcatcggg ctgttggtgg cgattccggt cggcatcatt tccgccatcc gccaggacac
     3001 gatcctcgat tacgcgaccc ggagcgtggg catcgggttc attgccatac cgacgttctg
     3061 gctgggaaca ctcgtcatgg tcattccgcc ggctctttgg ggttgggctc cgcctgcgcg
     3121 ctacttcgac ctctgggacc ggccgctcga caatctggag ctgatgatca tcccctcggc
     3181 gctactcggg gtggcgctct cgggcaccgt gatgcgcctc gtccgggcac agatgctcga
     3241 ggtcatgcgc caggactaca tccggaccgc ctggtcgaag ggcctgcggg agcggacggt
     3301 catcactcgg cacgcgatcc gcaacgcctt tatccccgtg atcacgctca taggcctgca
     3361 ggttccggta ctcatcggcg ggaccgtcgt gctggagaac atcttcagcg tgcccggcgt
     3421 tggccggtac ctcgtattcg ccattcagca gcacgattat cctgtggttc agggcgtgaa
     3481 cctgctaatc gccctcattg tcgtcgtcac gaatctggtg gtcgacatga cgtacggcat
     3541 cctcgacccg cgcgtgcggt atagctaggt agcgagatga atcgaacgga cgcggctccg
     3601 cggacggagg cgacaccggg gcttggcaca ccacagcgtg cgagccgagc agtccgctgg
     3661 ctacgctggc tttggtggtt catccgtaag aagccactgg gcgcctttgg cgcactgctc
     3721 atcctcatcc tgctcgccac agccgcgttc gcgcccctgc ttgcgcctca gcactacagc
     3781 gatcaggtgc tctccgaagc gctgatgggc ccttcgcggg accacctcct cggaaccgat
     3841 tgggtgggac gcgacctgct gagtcgcatc atctacggag cccgagtttc tatcgtcgtg
     3901 ggattcggcg ctgtcgccat ctcgacggtg ctctccacgg tgctcggcgt cgtaaccggg
     3961 tactacggcg gcctcgtcga tacgctcatc cagcgcgtcg tcgacatgtg gatcgcgttt
     4021 cccgccatcg tgctgctcat cagcatcatc gcggtgttcg ggacgggcgt gtcgcaggtc
     4081 acgatcgcgc tcggcatcct gctcgccgcc ggatcgacga gagtcgtgcg cagcgctgtc
     4141 atcgccatca agaacaacca gtacattgaa tcggcccgcg cgctgggcgc cggcgacctt
     4201 cgcatcctca cctggtacgt cgtgcccaac atcatggcga cgattatcgt gctcgccacc
     4261 gtccagctcg ggaccgcgat tctcgccgag gcgacgctga gcttccttgg ctacggcgtt
     4321 ccgccgcccc aaccatcgtg gggccagatg ctgagtctct cctcactcca gttcatgcgc
     4381 gatgttccag gactcgcgat tttccccggc gtcgctatcg ccctcgccgt gtacgggttc
     4441 aacatgctcg gcgatgcgct tcgggacgtg c
//
LOCUS       DATMBB010000310         4918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_431891, whole genome shotgun sequence.
ACCESSION   DATMBB010000310 DATMBB010000000
VERSION     DATMBB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431891"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VNL92_06675"
     CDS             <1..1505
                     /locus_tag="VNL92_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWO94431.1"
                     /translation="AVTEAEEFAKIYNLDVVVIPTHKEMIREDYSDLVFRNERAKFNA
                     VAQEIEECYQDGQPVLVGTVSIEKSEYLSELLKRRGIPHEVLNAKHHEREAQIIARAG
                     ERGAVTIATNMAGRGTDIKLGEGVDELGGLHVVGTERHEARRIDNQLRGRSGRQGDPG
                     SSRFYVSFEDDIMRKFAPDWLPGMMARLGMEEDMPLESGWVSRAIETAQTKVEGHHFD
                     IRKRLVEYDDVMNLHRETVYAERRKVLEGADIRANVLAMVHEELDAILDQHLQGDPDE
                     WDLLALVRDLSSVIPLEDDATQDLEGATAQEIATSVHAHADERYAERESELGAEAMRT
                     LERLTMLRTIDQLWVHHLTEMDEMRQGIGLRAYGQGDPLVHYKREAHDMYQQLTENIR
                     SHVARSIFRVQLVPARTARAPVATTEGRGAEPQPDGTGAPEGAPAAPPAQRAPVAAGV
                     GAAMSRGPTAVRTNRDAAPSATPSFGKVGRNEPCPCGSGLKYKKCHGASA"
     gene            1557..2312
                     /locus_tag="VNL92_06680"
     CDS             1557..2312
                     /locus_tag="VNL92_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94432.1"
                     /translation="MTEAEQAPSADEMRSHDADVIERMLAAIDDGEVWHKALLAAIAE
                     WQQPEELLSDGRTVRYLYGGDAFDWLALAERLCDELDGRAPPDEREALILRGALPEKL
                     NDEEFQRRIGAEKYSSHLNYLYGVQVEEALQLAVEEELLKELRSSAGGANGSIEDAAF
                     VRIYGKPVASLIETYCEETGGEITGDELTLTELREFTYWLFRYRVRRSEPARIASDTR
                     KGLAQLSRLEMARLTSPLHIHEAPHEVIEATVR"
     gene            2406..>4918
                     /locus_tag="VNL92_06685"
     CDS             2406..>4918
                     /locus_tag="VNL92_06685"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWO94433.1"
                     /translation="MTTARRRQDMPKAYEPHRAEGEMYRLWEASGAFTPQIDWSREPF
                     TIIMPPPNVTGELHLGHALTATIEDVLIRWHRMLGDPTLWLPGVDHAAIAVQNVVERE
                     LAKEGLTRHDLGREGFLERVWEWVGTYRNVITDQHKRLGVSADWSRERFTMDAGPQKA
                     VRTTFVNLYNDGLIYRGERITNWCPRCNTALSDLEVEHAAEASSLWYVRYPLLDDAGQ
                     PTSEYLTIATTRPETMVADVAVAVNPHDDRYGRFVGRQVLLPLMNRPIPVIADEAVQT
                     EFGTGALKITPGHDPVDFEIGERHDLPTISAIDLNGVLNAEAGPFEGMDRDRAREKIV
                     ERLREEGYLARVEPYEHAVGHCHRCGTVVEPLITPQWWLRIEPLAEPALAAVREGRIR
                     FVPERFERTYTNWMENIHDWCLSRQLWWGHRIPVWYCEACEQLTVTVDDPRTCAHCGG
                     SAIYQDPDTFDTWFSSGLWPHSTLGWPDDTDDLRYFYPTSVMETGYDIIFFWVARMIM
                     LSLYNMNGEVPFRVVYLHGLVRDEAGRKMSKTLGNVVNPLALVERFGADALRYTLATS
                     STPGNDMRLSESRLADGRNFANKLWNAARFVISSLPEGFEMTRPIPGTGRSVDDLWIL
                     SRLNAVTASVNQLLRDFQLGEAGRQVYEFLWGEYCDWYIEIAKVRLRQGDQTPLSVLI
                     HVIESGLRLLHPYMPFVTEAIWQELRPELPDADGDTLMTQRYPEANAAWQDERAEATL
                     QLTMDIVRAIRNARAEHGVEAARWVEAYIIAGDRLAELEPLAGAIEALARARPLHFVA
                     QASEAPRDNIVTGVLNDAEVVLPLAGLFDQDAERARLAK"
BASE COUNT          945 a         1658 c         1559 g          756 t
ORIGIN      
        1 ccgccgtcac ggaggccgag gagttcgcga agatctacaa cctcgacgtt gtcgtcatcc
       61 ccacccacaa ggagatgatc cgcgaggact actcggacct tgtcttccgc aacgagcgtg
      121 cgaagttcaa cgccgtggca caagagatcg aggaatgcta ccaggatggc cagccggtcc
      181 tggtcggcac cgtttcgatc gagaagtcgg aatacctctc cgagctgctg aagcggcgcg
      241 gcattcccca cgaggtcctg aacgcaaagc atcacgagcg cgaggcgcaa atcatcgctc
      301 gcgcgggcga acgcggagcc gtcacgattg ccacgaacat ggcggggcgc ggcaccgaca
      361 tcaagctggg cgagggggtc gacgaactcg gcgggctgca cgtcgtcggc acggagcgcc
      421 acgaggcgag acgcatcgat aatcagctcc gtggtcgttc tggccgccaa ggcgaccccg
      481 gcagctcgcg cttctacgtg tccttcgagg acgacatcat gcgcaagttc gcgcccgatt
      541 ggctgcccgg tatgatggcg cgccttggca tggaggagga catgccgctc gaaagcggct
      601 gggtctcgcg cgccatcgag acggcgcaaa ccaaggtgga gggtcaccac ttcgacatcc
      661 ggaagcgact cgtcgaatac gacgacgtga tgaatctgca ccgcgagacc gtctacgcag
      721 agcgtcgcaa ggtcctggaa ggcgcggaca tccgagcaaa cgtcctcgcc atggttcatg
      781 aggagctcga cgccatcctc gaccagcatc tccagggcga cccggacgag tgggacctgc
      841 tcgccctcgt ccgcgacctc tcgagcgtga ttccgctcga ggacgacgcg acgcaggacc
      901 ttgagggcgc cacggcccag gagatcgcga catctgtgca cgcccatgcc gacgagcgct
      961 acgcagagcg cgagagcgag ctcggcgccg aagcgatgcg caccttggag cgcctgacga
     1021 tgctgcgcac catcgaccag ctctgggtcc accacctcac ggagatggac gagatgcgcc
     1081 aaggcatcgg gctgcgggcc tatgggcaag gtgatccgct tgtgcactac aagcgcgagg
     1141 cgcacgacat gtatcaacag ctgacggaga acatccgcag ccacgtcgct cggtccatct
     1201 tccgagttca gctcgtcccc gcccggacag cgcgcgctcc ggtcgcgaca accgaaggcc
     1261 ggggtgccga accgcagccc gacgggacgg gtgcgccgga aggtgcgcca gcggcgcccc
     1321 cggcccagcg agcgcccgtc gcggccggcg tcggcgccgc aatgagccga ggaccgacgg
     1381 ctgttcgcac gaatcgcgat gcggccccat ccgccactcc atcgttcggg aaggtgggac
     1441 gcaacgagcc ctgcccgtgt ggtagcggcc tcaagtacaa gaagtgccac ggggcgagcg
     1501 catgacccac ccttgctttg acgtggagcc ccgccctgcc gcagcctcga acagccatga
     1561 ctgaagccga acaagcgccg agtgcagacg aaatgcgctc ccacgatgca gatgtcatcg
     1621 agcgcatgct cgccgccatc gatgacggcg aggtctggca caaggcactc ctcgctgcca
     1681 tcgccgagtg gcagcaaccg gaggaactgt tatcggatgg gcggaccgtg cgctatctgt
     1741 atggagggga cgccttcgat tggcttgcac tggccgagcg cctctgcgac gagctcgatg
     1801 ggcgcgcgcc accggacgaa cgcgaggccc tcatcctacg gggagccctc cccgaaaagc
     1861 tcaatgacga agagttccaa cgtcgcatcg gagctgagaa atacagctcc cacctcaact
     1921 acctctacgg agtgcaggtg gaggaggccc tccagctcgc cgtcgaggag gagctgctga
     1981 aggagctgcg cagtagcgcg gggggcgcaa acggctccat cgaagacgcg gctttcgtac
     2041 ggatctacgg aaagcctgtc gccagcctca tcgagacgta ctgcgaagaa acgggcggcg
     2101 agattaccgg cgacgagctg acgctcaccg agctgcgcga gttcacctat tggctgttcc
     2161 gctaccgagt tcgccggtca gagccggcac gcatcgcaag cgacacgcgg aagggactag
     2221 cgcagctcag caggctcgag atggcgcgcc tcacgtcgcc gctgcacatc cacgaagcgc
     2281 cccacgaggt catcgaggcg acggtgcggt gaggcgcagc gccaccgtgg cgcggccctc
     2341 catcgattga tcgaccctcg cccgcaatct agaatcggga gacgatcccc cagcgagagg
     2401 gccccatgac cactgcccgc cggcggcagg acatgcccaa ggcgtacgaa ccgcatcgtg
     2461 cagaaggcga gatgtaccgc ctctgggagg cctccggcgc cttcaccccg cagatcgact
     2521 ggtcgcgcga accgttcacc atcatcatgc cgccccccaa cgtcaccggc gaactgcacc
     2581 tcggccacgc ccttaccgcc accatcgagg atgtgctgat ccgctggcat cgcatgctcg
     2641 gcgatccaac cttgtggctg ccgggcgtcg atcatgcagc aatcgccgtg cagaacgtcg
     2701 tcgagcgtga gctggccaag gagggcttga cccgacacga cctcggacgc gagggcttcc
     2761 tcgagcgcgt gtgggaatgg gtggggacgt atcgcaacgt catcaccgac cagcacaagc
     2821 gcctcggcgt ctcggccgac tggtcgcgcg agcggttcac gatggatgcc ggcccccaga
     2881 aggccgtgcg caccacattc gtcaatctct ataacgatgg cctcatctat cgtggcgagc
     2941 gcatcacgaa ctggtgcccg cgctgcaaca ccgccctctc cgaccttgaa gtcgagcacg
     3001 ctgccgaagc atcgagtctc tggtacgtcc gctatccgct cctggacgat gcagggcagc
     3061 cgacgagcga gtaccttacc atcgcgacca cgcggccgga aacgatggtc gccgacgtgg
     3121 cggttgccgt aaacccgcac gacgaccgct acggtcgctt cgtcgggcgc caggtcctgt
     3181 tgcccctcat gaatcgaccg atccccgtca tcgccgacga ggcagtgcaa acggagttcg
     3241 ggacgggcgc cctgaagatc acgcccggcc acgaccccgt ggacttcgag atcggcgagc
     3301 gtcacgatct gcccaccatc tctgcgatcg acctgaacgg cgtcttgaac gcggaggctg
     3361 gtcccttcga agggatggat cgcgaccgcg cgagagagaa gatcgtcgag cgcctgcgag
     3421 aggagggata tctcgcgcgt gtggagccat acgagcatgc ggtcggccac tgccatcgat
     3481 gcggaacggt cgtcgaaccg ctcatcaccc cgcaatggtg gctgcgcatc gagcccctgg
     3541 ccgagccggc gctggccgca gtccgcgaag ggcgcatccg cttcgtcccc gagcgattcg
     3601 aacgcacgta caccaactgg atggagaaca tccacgactg gtgcctctcg cgacagctct
     3661 ggtgggggca ccgcatccct gtgtggtact gcgaggcctg cgagcaactc accgtaaccg
     3721 tcgacgatcc gcgaacgtgc gcccactgcg gcggctctgc catctaccag gaccccgaca
     3781 cgttcgacac gtggttctcg tcagggctgt ggccacactc cacgctcggc tggcccgacg
     3841 atacagacga cctgcgctac ttctatccga cgagcgtcat ggagacgggc tacgacatca
     3901 tcttcttctg ggtcgcccgc atgatcatgc tgtcgctgta caacatgaac ggagaggttc
     3961 cgttccgtgt cgtgtacctc cacggcctcg tccgcgacga agccggcagg aagatgagca
     4021 agacgctggg caacgtcgtc aacccgctcg cgctcgtcga gcgcttcggg gccgacgccc
     4081 tccgatacac cttggcgaca agcagcacgc ccggcaacga catgcgcctc tccgagagcc
     4141 gcctcgccga tggccgcaac ttcgcgaaca agctctggaa cgccgcccgc ttcgtcatct
     4201 cctcactgcc tgaggggttc gagatgacgc gcccgatacc cggcacaggg cgatccgtgg
     4261 acgacctctg gattctgtcc cgcctcaacg cggtcaccgc ttcggtgaac caacttctcc
     4321 gcgatttcca gctcggcgaa gcggggcgcc aggtctacga gttcctgtgg ggcgagtact
     4381 gcgactggta catcgagatc gcgaaggtgc gcttgcgcca gggcgaccag acaccgctgt
     4441 ccgtgctgat ccacgtcatc gagagtgggt tgcgactgct gcatccctac atgccgttcg
     4501 tcacggaggc catctggcaa gagcttcgac cggaacttcc cgatgccgat ggtgacacgc
     4561 tgatgactca gcgctaccca gaagccaacg cggcctggca ggacgaacgt gccgaggcga
     4621 cgctccagct caccatggat atcgtgcgag cgattcggaa tgcccgggcc gaacacggtg
     4681 tggaggcggc gcggtgggtg gaggcctaca tcatcgcggg cgatcggctg gccgagctcg
     4741 aaccgctcgc cggagccatc gaggcgctcg ctcgcgcccg tccgctgcac ttcgtggcac
     4801 aagcttcaga ggccccacgc gataacatcg tgaccggcgt cctgaacgac gctgaggtcg
     4861 tgctccctct cgccggattg ttcgatcagg acgcggagcg ggccaggctc gcaaagca
//
LOCUS       DATMBB010000311         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_389166, whole genome shotgun sequence.
ACCESSION   DATMBB010000311 DATMBB010000000
VERSION     DATMBB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389166"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNL92_06690"
     CDS             <1..355
                     /locus_tag="VNL92_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94434.1"
                     /translation="LPFDAEVMICRGREIARLIARDFFAGQPVRPDIVRFVSVLSRRP
                     RLRPSTPMSFPAGGKWLLKVLAREGRFVVGLYRRQMRAISYLGRVDRIFGVPATTRNW
                     NTITAIARVLGDGGI"
     gene            907..1833
                     /locus_tag="VNL92_06695"
     CDS             907..1833
                     /locus_tag="VNL92_06695"
                     /inference="COORDINATES: protein motif:HMM:NF044334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94435.1"
                     /translation="MRNRRLAAYLVVAVAAPLAVLIAALYVASNDGMGRGDIPGFAFW
                     SFVLLLPLFAVFRTGERWVSTRTASWRALLGFVVGGVCGVLFTVTLALGMGPWIGAFS
                     FPILYIWLITAGVSVLLGAVLLSRSPGVTPSLRDTAKWGGITVASFVLIGLLPVGLVF
                     GSAFIWNRAQAETYLVPEDFQGSVFVVFDQPSFPALPLVDGRRIIQVPSSGIVVTRSA
                     IAEGWKKPQVYRVRRDGSREPVTTDWDRRDTVPTSVHTHWLATRTAGVVNGVQQPTMR
                     YEAFTLGRHDKERELEDRADVVLDSLWKLYAR"
     gene            1931..>2767
                     /locus_tag="VNL92_06700"
     CDS             1931..>2767
                     /locus_tag="VNL92_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94436.1"
                     /translation="MSEKRNHWKEDTRAAVLELTSERRRAGYGELPIMLPRALLALLV
                     AVSTPRATIAAQGPDTSVRVAIGFGVDTLGTPNHEVFALWRAYLARRPTCGQPSPLWS
                     AAERARWPGADLLCSFVYQGFSNFTVLHLAPAVGLDSTYLIRTLVARVSGPERAVWPL
                     AVYRVYATREGGRWVLANALPRLTRRWHRETIGRITFVFPPTRSFVRARAESSAAFVD
                     SLARAFDVPAPASISYYFTDDLIETLGALGLDFFPLASDTAGGRSLGDLVFVGSSSDG
                     EGN"
BASE COUNT          410 a          858 c          914 g          585 t
ORIGIN      
        1 gcttcccttt gatgccgaag tcatgatctg cagagggcgg gagattgcca gactgatagc
       61 tcgcgatttt ttcgcgggcc agcccgtgcg acccgacatc gtccgcttcg tgagcgtcct
      121 gtccagaaga ccgcgcttga ggccgtccac gcccatgagc tttcctgctg gcggcaagtg
      181 gctgctgaag gttctggcgc gggagggccg gtttgtcgtc ggcctgtacc gacgccagat
      241 gagggccatc agctatctcg gcagggtgga ccggattttt ggagtaccgg ccacgacgcg
      301 caactggaat accatcaccg cgattgccag ggtgctgggt gacgggggga tctgatcgac
      361 gcgcgaggcg ttacccgaca tccaatgact agccttcttc gcagacttcg cggcgcgttg
      421 ggtatcgggg tgacttgggg tgtctcgtgg gccgtgctcg gcgcgcttct gggcatcatt
      481 gttggtctgg tcaggccttc tgacatcgac cccggcgaag ggccggccac gattgctcgc
      541 gtgttgggtg tagttggcct catatcaggt gtcggcttcg ctccgctcct ttcgctggcg
      601 gagagtggaa agtccattgg cgaactctcg ttgctacggg tagcactctg gggctttctg
      661 ggagcagcac cgattcccgc gttcactggg gcggatgcga gcatgggatt ggttagtggc
      721 agtctcggcg cgttgttcgc ggccgggtcc gtagcgacag cccgacgtgg agcgctctcg
      781 cccggtgaac cgcgtgagct gcggaacgat ggcgaaaacc gatcggcccg ggaagcgtag
      841 tgtgcgcggg cggcaccatg agggcctgca gcggatgcgc ctggcgacag gcggcactaa
      901 ccaaccatga gaaaccgtcg gctagcggca tatctcgtcg tcgccgtcgc cgctccgctg
      961 gctgttctga tcgccgccct ctacgtggcg tcgaatgacg gtatgggacg cggcgacatt
     1021 cctggattcg ccttctggtc gttcgtcctg cttctaccac tcttcgcggt gtttcgcacc
     1081 ggtgaacgct gggtgtcgac tcgcacggcg tcgtggcgcg ctctgctggg gttcgtggta
     1141 ggtggggtct gcggtgtcct cttcacagtc acgcttgctc taggaatggg cccgtggatc
     1201 ggggctttca gctttccgat tctgtacatc tggttgatca cggcaggagt gagcgttctg
     1261 ctcggcgcgg tgctcctctc ccgatctcca ggtgtgacgc cgagcctccg ggataccgct
     1321 aagtggggcg gcatcacggt cgcctcgttc gtgctcattg ggctcttgcc agtcggcctc
     1381 gtcttcggga gcgccttcat ctggaatcgc gcgcaggcgg agacgtattt ggtccccgag
     1441 gacttccagg gttccgtctt tgtggttttc gatcagcctt cgtttcccgc gttgcccctt
     1501 gtagatggtc ggcggatcat tcaggttcct tcgtccggca tcgtcgtcac gcgcagcgcc
     1561 atagcagaag gttggaagaa accacaagtc tatcgcgttc gccgcgacgg ctcgcgggag
     1621 ccggtaacga cggattggga ccgacgggat acagttccca cctcggttca cacgcactgg
     1681 cttgcgacca ggacggcggg tgtggtgaac ggagtccagc aacccaccat gaggtacgaa
     1741 gcgttcactc tcggtcgtca tgacaaggag cgagagctcg aagacagggc agatgtcgtg
     1801 ttggattcgt tatggaagct ctacgcccgg taaccgtgcc gcctcacacg cgcgtgacgc
     1861 tgacggcgcc gtcctgctgg acgggcggcg gctcgtctac cgcgcggcgc gcgagaaagg
     1921 acgcgcgctc atgtcagaaa agcggaatca ctggaaggaa gacacgaggg ctgctgtcct
     1981 tgaactgacc tcagagcggc gccgcgccgg ttacggcgaa ctgccgatca tgctcccacg
     2041 ggccctcctc gcactcctcg tcgccgtatc gacccctcga gcaacgattg cggcccaagg
     2101 gccggacacg agtgtgcgcg ttgctatcgg attcggcgtc gacacgcttg gaactccgaa
     2161 ccacgaagtc tttgcactgt ggcgcgccta cctcgcacgt cggcccactt gcggccagcc
     2221 gagtcccctc tggtcggccg ccgagcgagc gcggtggccc ggcgccgatc tgctgtgcag
     2281 tttcgtgtat cagggtttct cgaacttcac ggtgctgcac ctggcccccg ccgtcggtct
     2341 ggacagcacc tacctgattc gcaccctcgt ggctcgggtc tccggtcccg agcgggctgt
     2401 gtggccattg gccgtctatc gcgtgtacgc gacgcgtgag ggagggcgct gggtgctcgc
     2461 gaatgcgctt cctcggctga cccgacgatg gcatcgggag accatcggac gcatcacgtt
     2521 tgtgtttcca cccactcgat ccttcgtgcg ggcccgcgcg gagtcatccg ccgctttcgt
     2581 cgactcgctg gcccgcgcgt tcgacgtgcc tgcgccggcg agcatcagct actacttcac
     2641 ggatgacttg atcgagaccc tgggcgcact cggtctcgac ttcttcccgc tcgcctccga
     2701 caccgccggc gggcggtctc ttggcgacct cgtgttcgtc ggttcctcaa gcgacggcga
     2761 ggggaac
//
LOCUS       DATMBB010000312         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_513963, whole genome shotgun sequence.
ACCESSION   DATMBB010000312 DATMBB010000000
VERSION     DATMBB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513963"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VNL92_06705"
     CDS             <1..971
                     /locus_tag="VNL92_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HWO94437.1"
                     /translation="ALPLLFPGKIEADAARERLYIADSNHHRVLIATLDGRILDVIGS
                     GESGLEDGTFERATFWRPQGMAHDGDTLYVADAENHAIRRADLSARTVQTIAGTGEQA
                     LFRHGGGIAKEVPLSSPYDVALHKGTLYIAMAGTHQLWALDLASGRISPYAGDGGEDI
                     VDGPVAEARLAQPYGLQTDRELLYFADSETSAVRTAGIGPDARVETLVGQGLFEFGDR
                     DGAGPQARLQHVQGLALGDRVVYLADTYNHKIKLLGPRTHEVTTIAGTGERGYADGPA
                     SEARFNEPAGLCLVRGMLYIADTNNHVIRTLDLASATVSTLALTGL"
     gene            1038..3089
                     /locus_tag="VNL92_06710"
     CDS             1038..3089
                     /locus_tag="VNL92_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWO94438.1"
                     /translation="MPNRLANETSPYLLQHKDNPVDWYPWSDEAFARARAEDKPVFLS
                     IGYSACHWCHVMERESFENETIAELMNRNFINIKVDREERPDIDNLYMTAVQTLTGRG
                     GWPMSVFLTPDGRPFHGGTYFPPEDRHGLPGFPRVLQTVADVYHSRRDDVLRASNEIT
                     EAIRAQGLARHSAEPLTVDILDAAFRGMEEGFDRVEGGMGQAPKFPQPCTWEFVLRHH
                     HRTGSPQALEMVTLTLRKMARGGMYDQLGGGFHRYAVDGRWLVPHFEKMLYDNALLAP
                     LYLHAWLQTGEPLFRDVVEGTLGYVLREMTDPQGGFYSSQDADSEGEEGKLFVWTPAE
                     IDAVLGPDRGRILRIYYGVTEAGNFEGRNILSVPRSPESVAQELGLPVGDLLAAIEEA
                     RPLLLEARSRRVPPATDDKVLVAWNAMMLKAFADCGATLGRPDWVEVARRNADFLLGS
                     LVDGSGRLLRSWRSGSASRLKAYLEDYALLADALLALYEATFEQRWLDHARRLADAMI
                     DLFWDPGSEVFFDTGHDHEGLVVRPRDIFDNATPCGSSAAAAMLLRLSVFAADPDYER
                     RAIASLRSVRDMLDRAPTAFANWLSALDFALARVREVVIIGPPDDPRTSALLGTVRAT
                     FDPNRVLAGAPAPVDHAPSPLLAGRTMLDGAPSAYVCENYACQMPVATPEALAEQLRV
                     R"
BASE COUNT          518 a         1107 c         1059 g          512 t
ORIGIN      
        1 ccgccctacc tcttctgttt cctgggaaga tcgaggctga cgctgcacgc gagcggctgt
       61 acatcgccga ctccaatcac caccgcgttc tgatcgcgac gctcgatggc cgcatcctcg
      121 acgtcatcgg ctcgggcgag agcgggctgg aggacggtac gttcgagcgc gctacgttct
      181 ggcggccgca agggatggca catgacgggg acacgctgta cgtcgccgat gccgagaacc
      241 acgcgattcg ccgcgccgat ctcagtgccc gcaccgtcca gacgatcgcc ggcacgggag
      301 agcaggcgct cttccggcac gggggaggca tcgccaagga ggttcccctc agctcccctt
      361 acgacgtggc cctccacaag gggacgctgt acatcgcgat ggccggcacc caccagctct
      421 gggcgctcga ccttgcgtcg ggccgcatct cgccgtacgc gggcgatggc ggcgaggaca
      481 tcgtcgacgg accagtggcc gaggcgcgct tggcgcagcc ctatggcctc cagaccgacc
      541 gcgagctgct gtacttcgcc gacagcgaga caagcgccgt ccgtaccgcc ggcatagggc
      601 ccgacgctcg ggtcgaaaca ctggttggcc aagggctgtt cgagttcggc gatcgcgatg
      661 gcgctggccc acaggctcgg ctccagcacg tgcaggggtt ggcgctcggc gatcgggtcg
      721 tgtacctggc cgatacgtac aaccacaaga tcaagcttct cggcccccgt acccatgagg
      781 tcacgactat cgcggggacg ggtgagcgtg gatacgccga cggccccgca agcgaagcgc
      841 gcttcaacga gcccgcggga ctctgcctgg ttcgcggcat gctctacatc gccgacacga
      901 acaaccacgt gatccgcacg ctcgatctcg cctcggcgac cgtgtcgacg ctcgcgctga
      961 ccggcctatg acgggagccg ggatgatgcg gtaggctgga cgcatcgagc gcaaccgcgc
     1021 aacgcaggga ggcagccatg ccgaaccgcc tggcgaacga gacgagtccc tacctgcttc
     1081 agcacaagga caatcccgtc gactggtacc cgtggagcga cgaggccttc gcgcgcgccc
     1141 gggcggagga caagccggtg ttcctgagca tcggctactc ggcctgccat tggtgtcacg
     1201 tgatggagcg ggaatcgttc gagaacgaga ccatcgccga gctgatgaat cgcaacttta
     1261 tcaacatcaa ggtcgaccgt gaggagcggc ccgacatcga caatctatac atgacggcgg
     1321 tgcagaccct caccggccgt ggcggatggc caatgagcgt cttcctcacg cctgatggcc
     1381 gtccgttcca cggtggcacg tacttcccgc ccgaggatcg acacggcctg ccggggttcc
     1441 cgcgcgtcct ccaaaccgtc gccgacgtgt atcacagccg ccgggacgac gtgcttcgcg
     1501 cttccaacga gatcacggaa gccatccgtg cgcagggctt ggcgaggcac tcggccgaac
     1561 cgctcaccgt cgatatcctc gacgccgcat tccgcggcat ggaggaaggc ttcgatcgcg
     1621 tcgaaggcgg gatggggcaa gcaccgaagt tcccgcagcc ctgcacgtgg gagttcgttc
     1681 tgcgtcacca tcaccgcaca ggttcgcccc aagcgctcga gatggtgacc ctcacgctgc
     1741 gaaagatggc gcggggcggc atgtacgacc agctcggtgg cggcttccat cgctacgccg
     1801 tcgatggccg atggctcgtc ccgcatttcg agaagatgct ctacgataac gccctcctcg
     1861 cgccactgta tctgcacgct tggctgcaga cgggcgagcc gctgttccgc gatgttgtcg
     1921 aggggacact ggggtacgtg cttcgggaga tgacggaccc gcaaggaggg ttctactcgt
     1981 cgcaggacgc cgacagcgag ggcgaggagg gcaagctctt cgtgtggacg ccggcggaga
     2041 tcgacgcggt gcttgggccc gaccgcggcc ggattctgcg catctattac ggggtcaccg
     2101 aagcgggaaa cttcgagggc aggaacatcc tctccgtgcc gagaagcccg gagtccgtcg
     2161 ctcaggagct gggattaccc gtcggcgatc tcctcgccgc gatcgaggag gcgcgcccgc
     2221 ttctcctcga ggcgcgatcg cggcgcgtgc caccggccac cgacgacaag gtgctcgtgg
     2281 cctggaacgc gatgatgctg aaggccttcg cggactgcgg cgcgacgctg gggcgacccg
     2341 actgggtgga agtggcgagg cgtaacgccg acttcctcct cggctcgctc gtcgatggat
     2401 caggtcgcct gctgcggtcg tggcgcagcg gatccgcgtc gcgcctcaag gcgtatctcg
     2461 aggactacgc gctgctcgcc gatgcccttc tcgcgctgta cgaggccacg ttcgagcagc
     2521 gctggctcga ccatgcccgc cgcctcgccg acgcgatgat tgatctgttc tgggacccgg
     2581 ggagcgaggt cttcttcgac acggggcacg accacgaggg tctcgtggta cgtccgcgcg
     2641 acatcttcga caacgcgaca ccgtgcggca gctccgcggc cgcagcgatg ctgctgcgtc
     2701 tctccgtgtt cgccgccgac cctgactacg agcggcgcgc catcgcctcg ctgcgttccg
     2761 tgcgcgacat gctcgatcgg gcgcccaccg ccttcgcgaa ctggctgagc gccctcgact
     2821 tcgcgctggc gcgcgtgcgc gaggtcgtca tcatcggccc ccccgacgac cctcgcacgt
     2881 ccgcgctcct cggtacggtg cgcgcgacgt ttgacccgaa tcgcgtgctt gccggcgcgc
     2941 ccgcccccgt cgaccacgcc ccatcgcccc tgctcgccgg ccgtacgatg ctggatggcg
     3001 cgccctcggc ctacgtgtgc gagaactacg cctgccagat gccggtcgcg acgccggagg
     3061 cgctggccga gcagcttcgg gtgcggtgag cgagacctca ccccctggcc ccctctcccc
     3121 ggccgagccg cggagagggg gaacggcgca tggaacgcag tctccggacc tcaccccctc
     3181 ccgcagctgg gggcga
//
LOCUS       DATMBB010000313         5000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_485046, whole genome shotgun sequence.
ACCESSION   DATMBB010000313 DATMBB010000000
VERSION     DATMBB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485046"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VNL92_06715"
     CDS             complement(<1..270)
                     /locus_tag="VNL92_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWO94439.1"
                     /translation="MTPPNAAAYAALEVAKRRVDSLLLERGLAETREKARALVLAGSV
                     SSAGRRIDKPGQMLPEAASLQVAERRRYVSRGGEKLEAALAAFSVP"
     gene            complement(267..797)
                     /locus_tag="VNL92_06720"
     CDS             complement(267..797)
                     /locus_tag="VNL92_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94440.1"
                     /translation="MRPPPPARVRSRRLAFPGPAVSALVLATMLALIGWNPSPRVAAH
                     PGGLDASGCHTCRTNCEASGLVANEYHCGHGASAPAPAVAAGEPPEEGVGEVAAPRAS
                     DPEPTTRMANDAEAAQLAGANYRLRLFDPDAADNGDSQLVSGLRAAAVGAWIALGLAA
                     LAVAFRKARIGRWLSP"
     gene            complement(811..1485)
                     /locus_tag="VNL92_06725"
     CDS             complement(811..1485)
                     /locus_tag="VNL92_06725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWO94441.1"
                     /translation="MGTMVTFASNGGQAEGYLATPTAANGGGIIVIQEWWGLVDHIKN
                     IAERFASEGFVALAPDLYHGRQTTEPDEAQKLAMSLQMDQAAKDMSGAVDYLRGRPEV
                     TGGIGVVGFCLGGGLALYLASIRPEIAACVPYYGALMGATPDLSAVRAAVLGHYADHD
                     DFASPEVARGLEEQLRSHGREATFHIYPNTQHAFFNDTRPETYNADAARQSWERTLEF
                     FRSHLK"
     gene            complement(1564..2325)
                     /locus_tag="VNL92_06730"
     CDS             complement(1564..2325)
                     /locus_tag="VNL92_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HWO94442.1"
                     /translation="MDVTSPLGPLFAFWAGLVSILSPCVLPLIPVYITHLTGQTTQAM
                     SMAGAGGPSTATFSIRNNAAFPHAVAFVLGFSVVFVIMGASVGVVGYALQDRQDILER
                     IAGVLIIAMGLHVSGILKIPFLERTYEVDVGRDTPPGLTRSFLVGSAFSVGWTPCFGP
                     ILGTILGLAATSSTVWQGAILLVFYSVGFSIPFLLTGAAVGTATRALRAVRPYMGAVS
                     AVSGVVLIVAGILIFTGELASLNQYFDFFESDVTV"
     gene            complement(2397..4229)
                     /locus_tag="VNL92_06735"
     CDS             complement(2397..4229)
                     /locus_tag="VNL92_06735"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HWO94443.1"
                     /translation="MTQGDLRDALDDCLARMAEGASVDDVLERYPGLASELRPLLVEA
                     QRLRDLELAQPDPAARAANRLRFLRRGAELRRAHQQRSIRSVAAAWLRRSWQPSRTRI
                     AHAVTSFVLVFASAGGLTAWAATGAGADSPLYSVKILIEDARLRFASDGESRADLLLD
                     LLGERNEELRDLLAEGKTPSGRFLDRLESTSRQAAESIAEIEDAQVRQRADQLLSEEV
                     RLLTRMAPRMAGDQGERVSQLWEAASSQRVALVASTSRPSATPPAPPPSAGDAAVRVV
                     LGSARRVEVGAASPAMVQDGAVEIAGETYRVAPGVELPPDGAGRVIIADTESGETLVI
                     GVESVTDPAATSRVQGSVSEATSSDFWVGGQRVVLEATTIVTGGPIAPGARVLVEGLA
                     DVNGDLRASEVQVVPADDAADITTFAGIVIGREGQAWRIQDETQGEIVLVEDGGAELD
                     LRATDGAPAVGFYAQGEAVAAGDGILTILTLRLSLPPVSATPSPEVTPDASPTPTGTP
                     EAEATTDDAWTFEGVVASYVGDVLSFNSGTTVRLPATLSLPTLGPGDIVIVGLVNVPN
                     DLPLVASLQLVEEAIETATPTPTPTASPEPSPTAEPPQSPFSLD"
     gene            complement(4226..4834)
                     /locus_tag="VNL92_06740"
     CDS             complement(4226..4834)
                     /locus_tag="VNL92_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWO94444.1"
                     /translation="MRDLGGVRSTPAERTLPRTGELESEADLIARAQAYDADALSTIY
                     ETYYPRLYNYCRLQLGDAHLAEDVTSDVLLQVLEALREYRFRGTPFAAWVFRIARNRL
                     IDHHRRSNRRPQVELSDVLPSQSDGPPAITERAQEYESVRAAIGHLTEEQRQVIILKF
                     VEDLDNSAIAQVLGRSLGAVKSLQHRALVSLRKVLEREGLGG"
BASE COUNT          879 a         1664 c         1649 g          808 t
ORIGIN      
        1 cggcacggaa aaggccgcga gcgcggcctc cagcttctct ccgccccggc tgacgtaccg
       61 gcggcgctcg gccacctgga gcgatgccgc ttccggaagc atctgtccgg gcttgtcgat
      121 ccggcggccg gcgctgctca cgctccccgc gagaaccaat gcgcgagcct tctctcgcgt
      181 ttcagccaga ccacgctcca gcaggaggct gtcgactcgc cttttggcca cttcgagggc
      241 agcataggca gcggcgtttg ggggcgtcaa ggggagagcc agcgcccaat tcgcgccttg
      301 cggaacgcca cggcgagagc ggcgaggccc agcgcgatcc aggcgcctac ggccgcggcc
      361 cgcaggccgc tgaccaactg tgaatcgccg ttgtcggcgg catcaggatc gaaaagcctg
      421 agacggtagt tggcaccggc cagctgcgca gcttccgcat cgttcgccat gcgcgtggtc
      481 ggctcaggat cggaggcgcg cggagctgcg acctccccga cgccctcctc gggcggttcg
      541 cccgctgcca ccgcgggcgc tggtgcactg gctccgtgac cgcagtggta ttcgttggct
      601 accagtccgc ttgcctcgca gttcgtccgg caggtgtggc aaccagaagc atcgagccca
      661 ccgggatgag cggcaacgcg gggagaggga ttccacccga tgagcgcgag catagtcgcg
      721 aggacaagcg ccgagaccgc cgggcccggg aaggcaaggc gccgcgatcg cacccgcgcc
      781 ggaggtggag gacgcatgcc caacctctcc ctacttcaga tgcgagcgaa agaactcgag
      841 cgtccgctcc caggactgcc gggctgcatc ggcgttatac gtttctgggc gagtatcgtt
      901 gaagaaggca tgctgggtgt tgggatagat gtggaaggtg gcttcacggc cgtggctccg
      961 cagttgctcc tcgagtccgc gcgcgacttc cggcgaggca aaatcatcgt ggtcggcgta
     1021 gtgccctagc acggcggcgc gaacggccga gagatcggga gtcgcgccca tgagggcgcc
     1081 gtagtatggg acacacgcag cgatctcggg ccgaatgctc gccaagtaca gggcaagccc
     1141 tccgcccagg cagaagccaa ccactccgat tcctccggtg acctccggcc gtccccggag
     1201 ataatcgacc gcaccactca tatccttcgc ggcttggtcc atctgcaggc tcatggcgag
     1261 cttctgcgcc tcatctggct ccgtggtctg acggccgtgg tagaggtcgg gcgcgagagc
     1321 gacgaatcct tcggaggcga accgctcggc gatgttcttg atgtgatcga caagccccca
     1381 ccattcctgg atgacgatga tgccgcctcc gttggcggcc gtcggagtgg cgaggtagcc
     1441 ttcggcctgt cccccattgc tcgcgaacgt caccattgtg cccatgatcg cgtacctccg
     1501 tgcctgctgc gtctcggcat gcccgcgccg attctgcaca cgccaagggt ggatgtcaaa
     1561 cggtcagacg gtaacgtcgc tctcgaagaa gtcgaagtac tggttcagcg atgcgagctc
     1621 gccggtgaag atgaggatgc ccgcgacgat cagcacgacg ccactcacgg ccgataccgc
     1681 ccccatgtac ggacggacgg cccggagcgc tcgcgtcgcc gtgccgacgg cggctccggt
     1741 gaggaggaac gggatgctga acccaaccga gtagaacacg agcaggatag ccccctgcca
     1801 caccgtactc gaggtcgccg ccaggccaag tatcgtcccg aggatggggc caaagcacgg
     1861 cgtccatccc accgagaacg ccgatcccac caggaacgag cgcgtcaggc cgggcggcgt
     1921 gtcgcgcccg acatccacct cgtacgtacg ctcgaggaat ggaatcttca ggatgcccga
     1981 aacatgcagg cccatcgcga tgatgagaac acccgcgatg cgctccagga tgtcctgacg
     2041 gtcctgcagc gcatatccca caacgcccac cgaggctccc atgatgacga agacaacgga
     2101 aaagccgagg acgaaggcca cggcgtgagg gaaggcggcg ttgttccgga tgctaaatgt
     2161 cgccgtcgat gggccaccag cgcccgccat gctcatggcc tgcgtggttt gacctgtgag
     2221 gtgagttatg tataccggga tgagcggtag gacacaggga gacaagatag agacaaggcc
     2281 agcccagaac gcgaacagag gaccgagcgg actcgtgacg tccattgtgc ctacttcccg
     2341 gatgggcgga gcttgcgtga gatggcgtca gtatacgcgg cgtgtgtgcc cagccgctag
     2401 tcgagcgaga acggcgattg cgggggctcg gccgtcggcg agggttcggg cgaggccgta
     2461 ggcgtcgggg tcggggtcgc ggtctcgatc gcctcctcta cgagctgcag cgacgccacc
     2521 aggggaaggt cgtttggtac gttgacgaga cctacgatga cgatatcgcc gggaccgagc
     2581 gtaggcaggg acagcgtcgc aggcagccgg accgtcgtgc cgctgttgaa cgacaggaca
     2641 tcgcctacgt acgaggcgac gacgccctcg aacgtccagg cgtcatccgt cgtggcctcg
     2701 gcctccggcg tccccgtcgg cgtcggtgag gcgtcaggag tgacctccgg cgagggcgtg
     2761 gcggaaaccg gaggcagcga tagacgcagc gtcaggatgg tgaggatgcc atccccggcc
     2821 gcgacggcct cgccctgtgc gtagaagccg accgccggcg ctccgtccgt cgctcgcagg
     2881 tcgagctcgg ctccgccatc ctcgacaagc acgatctcgc cttgggtctc gtcctggatt
     2941 cgccaggcct gcccctcgcg gccgatcacg attcccgcga acgtcgtgat atccgcagca
     3001 tcgtccgcgg ggacgacctg aacttcggat gcgcgtagat cgccgttgac atcggcgaga
     3061 ccctccacca ggacgcgtgc accaggcgca atcggtccac ccgtaacgat cgtcgttgct
     3121 tcaaggacga cacgctgtcc cccgacccag aagtcgctgc tcgtggcctc ggagaccgac
     3181 ccctgcaccc ggctggtggc agccggatcg gtcaccgact cgacaccgat cacgagcgtc
     3241 tcgccgctct ccgtgtcggc aatgatgact cgcccagcgc cgtccggagg aagctcgaca
     3301 ccgggcgcga cacgatacgt ctcgccggca atctccacgg caccgtcctg gaccatggcc
     3361 ggagacgcgg cgccgacttc gacccgccgg gcgctcccca acacgactcg gacagcggcg
     3421 tcccccgccg atgggggcgg ggcgggcggc gtggcactgg gcctcgaagt gctggccacg
     3481 agagcgacgc gctgggagct cgcagcctcc cacagctgag acacgcgctc gccctggtcg
     3541 ccggccatac ggggcgccat ccgagtgagc agtctcactt cctcggagag cagctggtcg
     3601 gctcgctggc gcacctgtgc atcctcgatc tctgcaatag actccgccgc ctgccggctc
     3661 gtcgactcga gccgatcgag gaaccggccg ctcggcgtct tcccctctgc cagcagatcc
     3721 cgcaattcct cgttccgttc gccaagcagg tcgagcagca gatcagcgcg cgactcgccg
     3781 tccgaagcga atcgcaggcg cgcgtcctca atgaggatct tcacgctata cagcggacta
     3841 tcggcgcctg cgcccgtcgc cgcccaggcc gtcagcccac cagctgatgc gaataccagc
     3901 acgaacgacg taacagcgtg cgcgattcgc gttcggctcg gctgccacga gcgcctcaac
     3961 caagcggcgg caacgctgcg gatgctccgc tgttgatgcg cgcgccgaag ctccgctccc
     4021 ctccggagaa agcgcaacct gttcgccgcg cgcgccgccg ggtcgggttg ggccagctcg
     4081 agatcgcgca gcctttgggc ttcgactagg agcggacgga gctcgctggc aaggccgggg
     4141 tatcgttcga ggacgtcatc aacagaggcg ccctcggcca ttcgagcgag gcagtcgtcc
     4201 aaggcgtcgc ggaggtcgcc ttgtgtcatc caccaaggcc ttcccgctcc agcaccttcc
     4261 gcaacgatac gagtgcccgg tgctggagcg acttgacggc gccgaggctg cggcctagga
     4321 cctgcgcgat agcgctgtta tcgaggtctt ccacgaactt gaggatgatg acctgacgtt
     4381 gctcttcggt gaggtgaccg atcgccgcgc gcaccgactc atattcctgc gctcgttcgg
     4441 tgatggctgg tggcccgtcg gactgcgacg gcagaacgtc gctgagctct acctggggcc
     4501 gccggttgct gcgccgatgg tgatcgatga ggcgattacg ggctatccga aacacccaag
     4561 cagcgaacgg cgtgccacgg aatcggtact cccgcagcgc ttcgaggacc tgcagcagga
     4621 catcgctcgt gacgtcttcg gcgaggtgcg catcgcccag ttggagtctg cagtagttgt
     4681 agaggcgcgg atagtacgtc tcgtaaatcg tggaaagggc gtcggcatca taagcctgcg
     4741 ctcgcgcgat gagatcggcc tccgattcca gctcgccggt acgaggaagc gtgcgctcgg
     4801 cgggcgtgct ccgcacgcct ccgaggtcac gcatcgtccg aatcccggtt cggagggcta
     4861 ccatacttag aggaacgcac gaggcgatcg gaagatacgg tgacgatgtc cactggcttc
     4921 acccttcgcc ttcctcctcc catgtggtat cgccggcttc atcgggaatg ccgagaaggc
     4981 ggcgcctggc agcgtcgtat
//
LOCUS       DATMBB010000314         4448 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_568538, whole genome shotgun sequence.
ACCESSION   DATMBB010000314 DATMBB010000000
VERSION     DATMBB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568538"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..287
                     /locus_tag="VNL92_06745"
     CDS             72..287
                     /locus_tag="VNL92_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWO94445.1"
                     /translation="MVSSPFENYLDLVEAARELGIHPQSLRRLIKQKKVPAVLFAGKY
                     LIERDKLEMFKANYDPRPGRKPIRRLL"
     gene            399..1562
                     /locus_tag="VNL92_06750"
     CDS             399..1562
                     /locus_tag="VNL92_06750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWO94446.1"
                     /translation="MPLALLVGALAACGTDPSPIAIEEADPTRSATAPRPSQPEAATV
                     SIAAVGDVMFARTVGEAMVEGGLDAAFAGVGDTLREADIAFANLEMALTERGEPAPKD
                     FTFRAPPSFGPALVGAGIDVVSLANNHIMDYGPEGLFDTLVSLELLGVKGTGAGASRE
                     AAVKPAIVESKGIRVAFLAFASCRNDSVSGFEARSFEAGETTPGIAWAEVDRITQAVR
                     DARAQADVVVVSFHWGDEYRHEVSEEQQALAYTAVDSGAQLVLGHGPHVLQGWEARGE
                     ALIVYSLGNFVFDLDDVDRSVPGLPSVNTLVLQVEVGPSGTVSASYVPVLIDEATGFP
                     HRAEGPEELRVRQLVEALSPTVADAVAALQLSAQTRGASKGGVTRDPVAAPGN"
     gene            complement(1528..2184)
                     /locus_tag="VNL92_06755"
     CDS             complement(1528..2184)
                     /locus_tag="VNL92_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWO94447.1"
                     /translation="MTSRGNHRVPNLWLAKTLWEVGGVRFGDFTLGKTTVHSPVYVNP
                     RRLVSKPTALARAARVMDEEVRALMAMRNPPIQPFDLVAGIPFGGLHLATAFSLRTRT
                     PLIYLQPRPGHPGEQVVEGMYSPGQRVLLIDDLVNKGGSILRTANALEEAGLEVRNAV
                     VLIDRQVGARERLRERGYDVFCILGLEALLNYLMSTAEIEESVYRKSLSYLEQQRDHE
                     "
     gene            complement(2532..3518)
                     /locus_tag="VNL92_06760"
     CDS             complement(2532..3518)
                     /locus_tag="VNL92_06760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWO94448.1"
                     /translation="MRPRIFVTRQLPGDPLAQVRDRYEIDVWPEEGAPPYEMLLRKAA
                     ESDGLLVMLTDRIDAPLLGAAPRLRAVSTMAVGYDNIDVEAATTQGVAVCHTPGILTE
                     TTADLAFALLLAAARRVGEGERMLRAGRWRGWAPTQLLGRDVHGATLGIVGMGAIGQA
                     VARRALGFGMSILYTSRSEHPRVERELGARRLPLDAVLAASDFVSLHVALTPETRGLI
                     GERELARMQRHAVLINTARGPVVDQRALYAALREGRIAAAGLDVFETEPLPADDPLLA
                     LENVVLLPHIGSASVATRTAMARIAVENLIAALEGREPANCLNWQHLVRESR"
     gene            complement(3523..4065)
                     /locus_tag="VNL92_06765"
     CDS             complement(3523..4065)
                     /locus_tag="VNL92_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWO94449.1"
                     /translation="MADQRLIDELMAKLQEERDRILQQARSLTEEQASFAPDDAQGEA
                     QWTPKEQWAHMAEMETNYRAWIRAGLEQDDPDVGSVRGEPVAIPLTEANQHSNQELID
                     QLVREREQTLQLLRSIPAEQYDRTATNALFGTLTLLQWARSYYRHDRMHLDQIAGREP
                     EYKPKFAGGTEPDQRRRAPA"
     gene            complement(4169..>4448)
                     /locus_tag="VNL92_06770"
     CDS             complement(4169..>4448)
                     /locus_tag="VNL92_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94450.1"
                     /translation="TLEPQSFDEPVPSAIPLYPGGNLIAFPWGDRDATFVGAWLGDTL
                     TLALGWNAETGAWDAYLPAAPQASTLTSFEQFGVYWIVMTEGVDLALN"
BASE COUNT          717 a         1423 c         1494 g          814 t
ORIGIN      
        1 taacccttga tcgagactgg cgcccgcccc tatattggga aagcgcgtta gcgcctgggg
       61 taggaggcgg gatggttagc agtcctttcg agaactacct tgacctggtg gaagcggccc
      121 gcgagctggg catccaccca cagagcctgc ggcggctcat taagcagaag aaggtgcctg
      181 ctgtgctctt cgctggcaag tacctcatcg agcgggacaa gctcgagatg ttcaaggcga
      241 actacgatcc acggccgggc cgcaagccca tccgccgtct actctgaagc atcgggactc
      301 cagttcgcgc ccacttccgt tgtccagtat ggccggcccc gaccgccctg cgaagctggc
      361 caagcagacg cgccatgaga gcatgccggc gagccttctt gccgctcgcg cttctcgtgg
      421 gggcactcgc cgcctgcggg acggatccct cgccgatcgc catcgaggaa gctgacccca
      481 ctcgctccgc aacggccccg cgtccctcgc agccggaagc agccacggtc agcatcgccg
      541 ccgtcggcga cgtcatgttc gcgcgaaccg tcggagaagc gatggtcgag gggggcctcg
      601 acgccgcgtt cgcgggcgtc ggcgacactc tgcgtgaagc ggacatcgcc ttcgccaatc
      661 tcgagatggc cttgacggag cgcggcgagc cggcacccaa ggacttcacg ttccgcgcgc
      721 cgccgagctt cgggcccgca ctggtcgggg ccggcatcga tgtcgtgtcg ttggcgaaca
      781 atcacatcat ggactatggc cccgagggct tattcgatac gctcgtctcc ctggagttgc
      841 tcggcgtcaa gggcacggga gcgggagcgt cgcgtgaggc ggcggtaaag cccgcgatcg
      901 tggagagcaa gggcatccgc gtcgccttcc tggcgttcgc gtcttgccgc aatgattccg
      961 tcagcggctt cgaggcgcgc tcgttcgagg cgggcgagac gacgccgggc atcgcgtggg
     1021 cggaggtcga ccgtatcacg caggcggtcc gcgatgcgcg tgcgcaggcc gatgtcgtcg
     1081 tcgtgtcctt ccactggggc gacgaatatc gccatgaggt aagcgaggag cagcaggcgt
     1141 tagcgtacac cgctgtggat tccggcgcgc agctcgtgct tggtcatggg ccgcacgtac
     1201 ttcaggggtg ggaggcgcgt ggcgaagcgc tcatcgtgta cagcctcggg aacttcgtgt
     1261 tcgacctgga tgatgtggac cgctccgtgc ccggactccc gtccgtcaac acgctcgtat
     1321 tgcaggtcga ggtcggtccg agcggcacgg tgtcggcttc gtacgtcccc gttctcatcg
     1381 acgaggcgac gggattcccg catcgagctg agggtccgga ggaattacgt gtgcgtcagc
     1441 ttgtcgaggc gctgtcgccg accgtagccg atgcggtggc cgcactgcaa ctctctgcgc
     1501 agactcgagg agcgtcgaag ggtggtgtca ctcgtgatcc cgttgctgct ccaggtaact
     1561 gaggctcttg cggtagacgc tctcctcgat ttcggcggtg gacatgaggt agttcaggag
     1621 cgcttcgaga cccagaatgc agaagacgtc gtaaccgcgc tcgcgcagac gctcgcgcgc
     1681 tccgacctga cggtcgatga gaacgactgc gttacgcacc tcgaggccag cttcctcgag
     1741 cgcgttcgcc gtccgaagga tgcttccgcc cttgttcacg aggtcgtcga tgaggagcac
     1801 gcgctggccc gggctgtaca tcccttccac cacttgctca cctggatggc cgggcctcgg
     1861 ctgcagatag atgagcggtg tccgggttct cagcgaaaat gccgtggcga ggtggaggcc
     1921 accaaacgga atgcctgcta cgaggtcgaa cggttgaatg ggcgggttgc gcattgccat
     1981 gagggcgcgc acttcctcgt ccataacgcg tgcggcgcgg gcaagtgcgg tcggcttgct
     2041 gacgaggcgg cgagggttca catagacagg agaatgcacg gtcgtcttgc caagcgtgaa
     2101 gtcgccgaag cgaactccgc cgacttccca gagggtcttg gccaaccaca ggttgggaac
     2161 gcgatggttg ccgcggctgg tcatcgtgta caggttcggg gtgcggaaaa ggccgcgaca
     2221 ctgagacgcg gcctcccgac ggaaatttgt aactctagct tcattgggca ggccccatca
     2281 ccaggttcca cctgattggg tagccggtca tccttccatc gacttcggcc tgcgggtcct
     2341 cactgagagg cctgccgagc ggctcgccct gctcctgggc agggcctgct cagtcgactt
     2401 gccatactcc gaatcggact ctgtccggga ctccgtacct catggatccc tccttgaatt
     2461 ggagtggtcc aatggtcctc agcttcccga ggaggtttta actttacgat cgatggggac
     2521 caaagtcaag attaacgtga ctcccgcacg aggtgctgcc agttgaggca gttggccggc
     2581 tcgcggcctt cgagggccgc tatcaggttc tccacggcga tccgtgccat cgccgttcgc
     2641 gtcgccacgc tcgcgctgcc gatatgcggt agcaggacga cgttttcgag tgccaggagg
     2701 ggatcgtcgg caggaagcgg ctccgtctcg aaaacgtcca gtcccgcggc tgcgatacgc
     2761 ccttcccgga gtgccgcata gagcgcccgc tggtcgacga ctggcccgcg agcggtgttg
     2821 atgagcacgg cgtgtcgctg catgcgcgcg agctcgcgct cgccgatgag cccgcgcgtt
     2881 tcgggggtga gcgcgacatg caggctcacg aagtcggaag cggcgagcac cgcatcgagt
     2941 ggaagacgcc gcgcgccgag ctcgcgctcg accctcggat gctccgatcg gctcgtgtac
     3001 aggattgaca tgccgaagcc gagggctcgg cgggccacgg cctggccgat ggcgcccatg
     3061 ccaacgatgc ctagcgtcgc tccatgcacg tcgcgtccga ggagctgtgt tggcgcccag
     3121 ccgcgccatc ttcctgcccg cagcatccgt tcgccctcgc ctacgcgccg tgccgccgcg
     3181 agcagcaacg cgaaggcgag gtcggctgtg gtttccgtga ggatgcccgg cgtatggcac
     3241 acggcgacac cctgagtggt ggccgcttcc acatcgatgt tgtcgtatcc gacggccatg
     3301 gtcgaaacgg cgcggagtcg tggcgccgcg ccgaggagcg gcgcgtcgat cctgtcggtc
     3361 agcatcacga gcagaccgtc gctttccgcg gccttccgga gcagcatctc gtagggcggc
     3421 gcgccctcct ccggccacac gtcgatctcg tagcggtccc gaacttgcgc gagaggatcg
     3481 cccgggagct gacgcgtaac gaagatgcgc ggccgcaccg gctcaggcgg gagcgcggcg
     3541 ccgctggtcg ggctcggttc cgcccgcgaa cttcggcttg tactccggct cgcgccccgc
     3601 tatctggtcg agatgcatgc ggtcatgccg gtagtacgag cgtgcccact ggagcagcgt
     3661 gagagtcccg aatagcgcat tcgtcgccgt tcgatcgtac tgctccgctg ggatggagcg
     3721 aagcagttgg agcgtctgct cgcgctcccg cacgagctga tcgatgagct cttggttcga
     3781 gtgctggttc gcctccgtga gagggatcgc caccggctcg ccccggacgc tgccgacatc
     3841 cgggtcgtcc tgctcaagcc cggctcgaat ccaggcgcgg tagttcgtct ccatttccgc
     3901 catgtgcgcc cattgctcct tgggcgtcca ttgcgcttcg ccctgcgcgt cgtccggggc
     3961 aaagctcgct tgttcctccg tgagcgaccg ggcctgttgc agaatccgat cgcgctcctc
     4021 ctgcagcttc gccatgagct cgtcgatgag gcgttggtcg gccatatcgc acctcctccg
     4081 gctcgcatcg ccggcggggc gatactacgc catgttggca agcggcacaa tggaccgtag
     4141 gtgcgcggcg acggtgtgga ggggaacgtc aattgagcgc gagatcgacg ccttcggtca
     4201 tgacgatcca gtacaccccg aactgttcga atgaggtgag cgtggacgct tgtggcgccg
     4261 cggggaggta ggcatcccat gcgcccgttt ccgcattcca ccccagtgcc aaggtcagcg
     4321 tgtccccgag ccaggccccg acgaatgtag catcacgatc gccccagggg aacgcgatca
     4381 ggttgccgcc ggggtacagc gggatggccg aaggaactgg ctcgtcgaag gactggggct
     4441 cgagcgtc
//
LOCUS       DATMBB010000315         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_131066, whole genome shotgun sequence.
ACCESSION   DATMBB010000315 DATMBB010000000
VERSION     DATMBB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131066"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VNL92_06775"
     CDS             <1..1061
                     /locus_tag="VNL92_06775"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWO94451.1"
                     /translation="DVEVGSDADPNPAAPQEPNTGLIDLSADAGGGPGRWMGGDLHCH
                     TLHSDGRNTVEQIVLSAIERGLEFLAVTDHNTVTHHAELERLSHLPIVLIPGEEVTTY
                     YGHTNAWGLAGRWVDFRCVDGPGIRAIAEFVLSQGGLCSVNHPKGPGSNWRFETAAGY
                     PCMEVWQAPWQHRNWESVARWEHEVAAGQRIVMVGGSDVHSIPPAPPLHPHGLGNPTT
                     WVYVRGAPSPSSVLEGIRRGHAFISADPSGPRIELTAHADGDTQTGMAGDALHLEDDR
                     PIRVRARVSGASGKYLCLIADGNDFSRLYLSRDDETQEMWLHPGGRRYVRAEVRDHGA
                     GESATVWAMSNPIWFERG"
     gene            1078..2037
                     /locus_tag="VNL92_06780"
     CDS             1078..2037
                     /locus_tag="VNL92_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWO94452.1"
                     /translation="MTLPEEAVGSVMRPRGEGALRGASWWARTRLREGGLAIALLLPS
                     LVLFGLFTFYPLIRSAYLGFFRSDPFGRASTYVGFEQYADVLGSEAFRESLRVTALFA
                     LYTVPTGLVLGLTLALLAHQRLRGITIYRTIFSSTIASSVAVASVMWLSLLNARIGVL
                     NYVLREVGADPIDWLTDPAWALPAVSLTTIWLNLGFTCVVMLAGLQRIPEELYEAVKV
                     DGGGVLARFWHVTLPMLSPTLLFGVVVLTILAFQSFGQIDILTGGGPLGETNVIVYSI
                     YTDAFKDFNEGQAAAQATILFGIILAFTLAQLWLLERRVFYAE"
     gene            2027..2893
                     /locus_tag="VNL92_06785"
     CDS             2027..2893
                     /locus_tag="VNL92_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWO94453.1"
                     /translation="MRSEPVGESAPQTAKVQRRIGTTIQYVLLTLAAIVVLFPMYLAV
                     VNSLHPLEKIFSYPPSLFVTDPQWSNYVDVFDIAPIGRYLVNSFLVSALITVGQVATS
                     VLAAYAFAFLRFPLRTVLFALFLSTLMVPWEVTIIPNYETVESLGWLDSYQGLTVPFL
                     ATAFGTFLLRQAFLSIPRDLLDAAALDGAGHLGVMRHVAIPLGRPAIAALAVFSFLSA
                     WNQYLWPLLITNDDTYRTVQIGLKTLASQSVDSVNLVMAGTVIAAAPTFILLVVFQRH
                     LIRGISAGGVKG"
     gene            2896..>3482
                     /locus_tag="VNL92_06790"
     CDS             2896..>3482
                     /locus_tag="VNL92_06790"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWO94454.1"
                     /translation="MRFGRWFRRYSLRAVALVALAAPILAACAGDDDDDEGGASAASS
                     CPQGTLAGRTSPVEITFWHSMNAANEQTLQRLTDEFNASQDEVRVELVYQGTYTESRN
                     KYLTALRGGELPDLIQLEDTSTQLMIDSGTTLTIQECVDAEGYDLSDYLERVVAYYTV
                     EGTLASMPFNVSNPVLVYNKTAFQEAGLDPAFNSA"
BASE COUNT          556 a         1127 c         1110 g          689 t
ORIGIN      
        1 tcgacgtgga ggtgggcagc gacgccgatc cgaacccggc cgcgccccaa gaacccaaca
       61 cgggtctcat cgatttatcc gcggacgccg gcggcggacc gggccgctgg atgggcggcg
      121 accttcactg ccacacgctc cattcggatg ggcgcaacac ggtggagcaa atcgtgctca
      181 gcgccatcga gcgtggcctg gaattcctgg ccgttacgga ccacaacacc gtgacccacc
      241 atgcggagct ggaacgcctc tcccaccttc ccatcgtttt gataccgggc gaggaggtga
      301 ccacgtatta cggtcacacc aatgcctggg ggctggctgg gcggtgggtg gacttccgct
      361 gcgtcgatgg gcctggtatt cgcgccattg ccgaattcgt cctcagccaa ggcgggctct
      421 gctccgtcaa ccatccgaag ggacctggtt cgaactggag attcgaaacg gcggccggct
      481 acccgtgcat ggaggtgtgg caggcgccat ggcaacaccg caactgggaa tcggttgctc
      541 gttgggagca tgaggtggcg gccgggcagc gcatcgtcat ggtcggcggt tccgacgtcc
      601 actcgatacc gcccgctccg cccctccacc cgcatggtct gggcaatccg acgacatggg
      661 tctacgttcg cggggcgcca tcgccgtcga gtgtcctcga aggcatccgc agggggcacg
      721 cgttcatatc agcggatccg tccggtccgc gaatcgagct tacggcgcat gcggatggcg
      781 atacgcaaac cggcatggcc ggcgacgcgc tccatctcga ggatgaccgg cccattcgcg
      841 tgcgagcgcg ggtgagcggg gcctcgggga agtacttgtg tctcatcgct gatgggaacg
      901 atttctcacg gttgtacctg tcgcgcgacg acgagacgca agagatgtgg ctgcacccag
      961 gaggacggcg ttacgtgcgc gccgaagtgc gcgaccatgg ggccggcgaa tcggcgaccg
     1021 tttgggcaat gtcgaacccc atctggttcg agcggggctg atgggacggc gcgcgatatg
     1081 accctccccg aagaagcggt tggatccgtc atgcggcctc gcggcgaggg cgccctgcgg
     1141 ggggccagct ggtgggctcg tacaaggctc cgcgagggcg gcctggccat cgccctgctc
     1201 ctcccatccc tcgtgctgtt tggcctcttc accttctacc cgctcatccg ctcggcgtac
     1261 ctcggcttct ttcggagcga cccgttcggg cgcgcttcca cctacgtagg cttcgagcag
     1321 tatgccgacg tcctgggttc ggaggcgttc agggaaagcc tccgggttac ggccctgttc
     1381 gcgttgtaca cggtgccgac cgggctcgtg ctgggcctca cgctcgctct cctggcgcat
     1441 cagaggctgc ggggcatcac gatctaccgc accatcttct cctccacgat cgcgtcatcg
     1501 gtggccgtgg catcggtgat gtggctttcg ctgctgaacg cccgcattgg cgtactcaac
     1561 tacgtcctgc gcgaggtggg tgcggacccc atcgactggc tcaccgatcc ggcctgggcg
     1621 ctgcccgccg tgtcgctcac gacgatatgg ctcaatctgg gcttcacgtg cgtggtgatg
     1681 ctggcgggac tccagcgcat ccccgaggag ctgtacgagg cggtcaaggt ggatggcggc
     1741 ggcgtcctgg caagattctg gcacgtgacg ctcccgatgc tttcgcccac gctgctcttc
     1801 ggcgtcgtcg tcctcaccat tctcgcgttc caatcgttcg gccaaatcga catcctgacc
     1861 ggtggcggcc cccttgggga gacgaacgtc atcgtctact cgatctatac ggatgctttc
     1921 aaggacttca atgagggcca ggcagcggcc caggccacca tcctgttcgg catcatcctc
     1981 gcgtttacgc tggcgcagtt gtggctgctg gagcggagag tcttctatgc ggagtgagcc
     2041 agtgggagag tcggctcccc agaccgcgaa ggtccagcgg cgcatcggaa caacgattca
     2101 gtacgtgctg ctcacgctgg cggccatcgt cgtgctcttc ccgatgtacc tggccgtcgt
     2161 gaactcgctc caccctctcg agaagatctt ctcgtacccg ccgtcgctct tcgtcaccga
     2221 tccgcaatgg tcgaactatg tcgatgtgtt cgatatcgct ccgatcgggc gctatctggt
     2281 gaacagcttc ctcgtgtcgg cgctgatcac ggtcggccag gtggcgacat cggtcttggc
     2341 ggcgtacgcc tttgccttcc tccggttccc tctgcgtacg gtcctctttg cgctgttcct
     2401 gtccacgctc atggtgccct gggaggtgac gatcatcccg aactacgaga cggtcgagag
     2461 cctaggatgg ctcgattcgt atcaaggcct gacggtcccc ttcttggcga cggcgttcgg
     2521 cacctttctt cttcggcaag ccttcctgag catcccgcgc gacctgctcg atgctgcggc
     2581 gcttgatggc gcgggtcacc tcggcgtgat gcggcacgtt gcgattccgc tcggccggcc
     2641 ggccatcgct gcgctggccg tgttctcgtt cctctccgca tggaaccagt acctctggcc
     2701 gctgctcatc acgaatgatg atacgtatcg cacggttcaa atcggcctga agaccctcgc
     2761 ttcgcaaagc gtcgattctg tgaatctggt catggccggc acggtgatcg cggccgcgcc
     2821 gacgttcatc ctcctggtcg tgttccaacg ccatctcatc cgcggcatct ccgccggtgg
     2881 ggtaaagggg tagatatgcg cttcggacga tggtttcgac ggtactcgct ccgggcggtg
     2941 gcgctggtcg cgttagcggc gcccatcctg gctgcctgcg ccggcgatga tgacgacgat
     3001 gagggcggcg ccagcgcagc gagcagctgc cctcagggaa cgctcgcggg gcggacctcg
     3061 ccggtcgaga tcacgttctg gcactcgatg aatgcggcca acgagcaaac gctgcaaagg
     3121 ctgaccgacg agttcaatgc ctcgcaggac gaggtgcgcg tcgagctcgt gtatcagggc
     3181 acgtacacgg aaagccgcaa caagtacctg acggcccttc gaggaggcga gctcccggac
     3241 ctgattcagc tcgaggacac atcgacccag ctgatgatcg acagcggcac gacactgacc
     3301 atccaggagt gtgtcgacgc ggaggggtat gacctgtccg actacctgga gcgggtcgtc
     3361 gcctactaca cggtcgaggg aacgctcgcc tcgatgccgt tcaacgtttc gaatcccgtg
     3421 ctcgtctaca acaagacggc cttccaggag gctgggctcg accccgcctt caacagcgcc
     3481 tt
//
LOCUS       DATMBB010000316         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_409099, whole genome shotgun sequence.
ACCESSION   DATMBB010000316 DATMBB010000000
VERSION     DATMBB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409099"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VNL92_06795"
     CDS             <1..210
                     /locus_tag="VNL92_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWO94455.1"
                     /translation="LNAGQHCDFFEPIRPGDTITATSAVSDVYEKTGRSGRMVFIVRT
                     TTYKNQRGEQVAVVNQSMVRRNVET"
     gene            207..668
                     /locus_tag="VNL92_06800"
     CDS             207..668
                     /locus_tag="VNL92_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94456.1"
                     /translation="MIQSVYGNQVEFGDEIGPLIKAPTTEDVQAFLSAWMTRDASDTS
                     VAGRFTDAQAASREGLAGPIIPGNMTQAFLGQLLMDWASPLGRLRSLDVNFRRPAMHG
                     QELKCMGLVTDTREEGDFTVVKLDVYAEAANGDRPVQGTAEVVLPTAPPVG"
     gene            675..1586
                     /locus_tag="VNL92_06805"
     CDS             675..1586
                     /locus_tag="VNL92_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO94457.1"
                     /translation="MTTDYRDLMAAAAQAAGIAPQAIVQPEDRDVVLGGLRFHYLDWN
                     QDHLPHAVFLHGGGLTAHTWDMAALLMRDKLHIVALDQRGHGDSDWTPNVEDDTASLM
                     LEDTRAFIEHLGYEKLTLVGMSMGGMNAIRYAARYPERLANLVIVDVGPDVMHAGVIE
                     LEQFAQATETLDRFDDFLDRSIKFNPNRPVEHLRYSLTHSLRQTDDGKWTWKHDRRPR
                     PNMEADALRERGERAASDLWDDVRAIRTRTLLLRGEKSNILSQESAERMVREMHDARL
                     AVIPGAGHSVQGDNPSAFAQAVLAFLS"
     gene            1642..2046
                     /locus_tag="VNL92_06810"
     CDS             1642..2046
                     /locus_tag="VNL92_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94458.1"
                     /translation="MRHRRRNEQVIRYYRYVSFITLTLVFVQAVLAGRFLFVEGDALD
                     VHEFVGNGITVVVIVQLMLSLAAGFAPALRLPLWTSILVILVVAQTGLGYVGRDNADA
                     ASIHVPTGVLLFGLAMLVAVLAFLDREEEEER"
     gene            complement(2052..>2260)
                     /locus_tag="VNL92_06815"
     CDS             complement(2052..>2260)
                     /locus_tag="VNL92_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2088 domain-containing protein"
                     /protein_id="HWO94459.1"
                     /translation="DDAALRFAFKPYPPARARSVLVRDTAHLELMYVSEALESDVAAH
                     PALERVGDYRALEFDSAGRLVAFE"
BASE COUNT          410 a          755 c          710 g          385 t
ORIGIN      
        1 ctcaatgcgg gccagcactg cgacttcttc gagccgattc gcccaggcga tacgatcacg
       61 gcgacctcgg ccgtgagcga cgtgtacgag aagacgggcc gtagcggtcg catggtcttc
      121 atcgtgcgga cgacgaccta caagaatcag cgcggtgagc aggtggccgt cgtgaatcag
      181 tccatggttc gaaggaacgt cgagacatga tccaaagcgt gtacggcaat caggtcgagt
      241 tcggcgatga gattgggccc ctcatcaagg cgccaaccac ggaagacgtg caggcgttcc
      301 tttccgcctg gatgacgcgc gacgcatcgg acacgagcgt ggccgggcgc ttcaccgatg
      361 cgcaggccgc gagccgcgag ggtctggccg ggccgatcat cccggggaac atgacgcagg
      421 cgttcctcgg gcaactcctc atggactggg cctcgcccct cggccgactg cggtcgctcg
      481 acgtcaattt ccgtcgtccg gcgatgcacg gccaggagtt gaagtgcatg ggactcgtca
      541 cggacacgcg cgaggagggc gacttcaccg tcgtcaaact cgatgtgtac gccgaggccg
      601 cgaacgggga ccggcccgtg caaggtacgg ccgaggtcgt tcttccaaca gcgccgccgg
      661 tcggatagct ccgcatgacg accgactacc gcgacctcat ggccgcagcc gcgcaggcag
      721 ccggcatcgc cccccaggct atcgtccagc ccgaggatcg cgatgtcgtg ctaggtggcc
      781 tccggttcca ctacctcgat tggaaccagg accaccttcc gcatgcggtg tttctccacg
      841 gcggcggcct cacggcccac acatgggaca tggctgccct cctcatgcgc gacaagctcc
      901 acatcgtcgc gctcgaccag cggggtcacg gcgattccga ctggacaccg aatgtcgagg
      961 atgacacggc gtccctcatg ctcgaggaca cacgcgcctt catcgaacac ctcggctacg
     1021 agaagctcac gctcgtcggc atgtcgatgg gtggtatgaa cgcgattcgc tacgccgcgc
     1081 ggtacccgga gcgtctggcc aacctcgtta tcgtggatgt tggtcccgac gtgatgcacg
     1141 ctggcgtcat cgagctagag cagttcgcac aggcaacgga aacgctcgac cggttcgatg
     1201 acttcctcga tcgctccatc aagttcaacc ccaaccgccc cgtcgagcac cttcgctata
     1261 gcctgaccca cagcctccgg cagaccgacg atggcaagtg gacgtggaaa cacgaccgtc
     1321 gcccgcgacc caacatggag gcggacgcgc tgcgtgagcg cggcgagcgc gccgcgagcg
     1381 acttgtggga cgatgtgcgc gccattcgaa cgcggacgct gctactccgc ggggaaaaga
     1441 gcaacatcct ctcgcaggag tccgcggagc gcatggttcg cgagatgcat gatgcgcggc
     1501 tcgccgtcat tcccggcgcc ggtcactcgg tccaaggcga caaccccagc gccttcgccc
     1561 aggcagtgct cgcgttcctg agctagcccc tgacaccccg ctgcgatacg atgaactccc
     1621 attccggacg aggaggattg cgtgcgccat cgtcgccgga acgagcaggt catccggtac
     1681 tatcgttatg tctcattcat cacactgacg ctcgtgttcg tccaggcggt acttgccggg
     1741 cggttcctct tcgtagaagg cgacgcgcta gacgtccatg agttcgtcgg aaacggcatc
     1801 accgtcgtcg tcatcgttca gctcatgctc tctttggcgg cgggcttcgc tccggcgctc
     1861 cggctgccgc tgtggacaag tatcctcgtg atcctcgtag tcgcacagac gggcctcggc
     1921 tacgttgggc gggacaacgc cgacgccgca tcgatccacg tcccgacggg cgtgctcctc
     1981 ttcgggctcg cgatgctggt tgccgtcctc gccttcctcg acagggagga agaggaggag
     2041 cgataggctc cttactcgaa cgcgacgagg cggcctgcgc tatcaaactc cagggcgcga
     2101 tagtctccca cgcgctccag agccgggtgg gccgcaacgt cggattccag cgcctcgctc
     2161 acgtacatca actccaggtg agcagtatcc cgcacaagga cggagcgcgc gcgcgcgggc
     2221 ggatacggct tgaacgcgaa ccgcagcgcg gcgtcgtccg
//
LOCUS       DATMBB010000317         3346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_425860, whole genome shotgun sequence.
ACCESSION   DATMBB010000317 DATMBB010000000
VERSION     DATMBB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425860"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VNL92_06820"
     CDS             complement(<1..529)
                     /locus_tag="VNL92_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HWO94460.1"
                     /translation="MPFAIVEGEPVTELPRDLYIPPYALEVFLEAFEGPLDLLLYLIR
                     RQNIDILDIPIAEISRQYVMYIELMKELQFELAGEYLVMAATLAEIKSRMLLPRSAEA
                     TEEEEDPRAELVRRLQEYERFKKAAEDIDALERLERDVLQASAEVVERQVVTKLPDVT
                     LKEMLIVFQEALQRSK"
     gene            complement(632..1303)
                     /locus_tag="VNL92_06825"
     CDS             complement(632..1303)
                     /locus_tag="VNL92_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HWO94461.1"
                     /translation="MPAIDIEKILRVLSVALIPGLFAITVHEVSHGWVARRFGDPTAY
                     MLGRLTLNPLKHIDPLGTVIVPIAMLVMSGGAWAFGWAKPVPVAFNNLRNPRRDMVLV
                     AAAGPISNFLMATAWALFAALQLNLLGGTTGVEQWLLGMCVFGITINVILAVFNFIPI
                     PPLDGGRVLSGLLPPSMSRVLDQIEPYGFVIVIVLMVMGVLWTLMEPFMLFFNDLFWS
                     LAGLR"
     gene            complement(1340..1963)
                     /locus_tag="VNL92_06830"
     CDS             complement(1340..1963)
                     /locus_tag="VNL92_06830"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWO94462.1"
                     /translation="MAQYFKVHPLNPQRRLLQRAAEIMRDGGVIVYPTDSCYAFGWRL
                     GAKEPLERVRRLRRTERDHEFTLVCRDLAEIATYARVDNWAYRLLRSLTPGPYTFVLR
                     ATHEVPKRLQDPKRRSIGIRVPDNKIAQCLIEELGEPIMSSTLILPGDPLPLTDPEEI
                     RDRLGKLVDAIVDGGAGGVEPTSVLDLSRDEVSVLRQGKGDVTPFVS"
     gene            2039..2584
                     /locus_tag="VNL92_06835"
     CDS             2039..2584
                     /locus_tag="VNL92_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HWO94463.1"
                     /translation="MQLLYDFFPVLVFFVAYYLGGIYVATAAIIVAMAVQIAVEYLRK
                     RKVSKMLLVSGGLVLVAGALTLALRNPLFVQWKPTIVNWLFAAAFLGTHFIGDKTLVE
                     RVMGHAIELEQPLWRQLNLMWVANFLFLGAANLYVVYNFSEEAWVNFKLFGMLGFTLL
                     MAVGQAIWISAKTAQAKQEES"
     gene            2589..2888
                     /locus_tag="VNL92_06840"
     CDS             2589..2888
                     /locus_tag="VNL92_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWO94464.1"
                     /translation="MLYAFICRDVPNSLPLRQATRPRHLEYVRPLVEQGRMVFAGPHP
                     AIDSPDPGPAGFTGSLIIAEFESLEAAKAWSASDPYVLEGVFAEVEIKPVLQTLP"
     gene            2885..3151
                     /locus_tag="VNL92_06845"
     CDS             2885..3151
                     /locus_tag="VNL92_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005417632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWO94465.1"
                     /translation="MTTDRVQLIRERLTAAFEPEILDVRDDSHLHAGHLGAREGKGHF
                     AVTIVSRRFQGKSILECHRMVYEALGDLMLTDIHALQVVARGAP"
BASE COUNT          577 a         1093 c         1098 g          578 t
ORIGIN      
        1 tctttgagcg ctggagcgcc tcctggaaga cgatgagcat ctccttgagc gtgacgtccg
       61 gcagcttcgt gaccacttgc cgctccacca cttcggcgga ggcctgcagc acgtcgcgct
      121 cgaggcgctc gagcgcgtcg atgtcctcgg cggccttctt gaagcgctcg tattcctgca
      181 gccggcggac gagctccgcc cgcgggtctt cctcctcctc cgtggcctcg gccgagcggg
      241 gcagcagcat gcgcgacttg atctcggcca gcgtggcggc cattacgaga tactccccgg
      301 cgagctcgaa ctgcagctcc ttcatgagct cgatgtacat cacgtactgg cgcgagatct
      361 ccgcgatggg gatgtcgagg atgtcgatgt tctgccgccg gatgaggtac agcagcagat
      421 cgagcggccc ctcgaacgcc tcgaggaaca cctcgagcgc gtacggcggg atgtagaggt
      481 cccgcggcag ctcggtgacg ggctccccct ccacgatcgc gaagggcatc tcggcctggg
      541 caggcgcgcc ggcctcggcc acgaccgggg gcggcacttg aggcaccagg cgcaggtttg
      601 gtcggccggc gggcttcggt gcttcagtca atcaacgcaa ccctgcaaga gaccagaata
      661 agtcgttgaa gaacaacatg aacggctcca tcagagtcca gagcacgccc atgaccatga
      721 gcacgatcac gatcacgaat ccgtatggct cgatctgatc gagcacgcgc gacatgctgg
      781 gcggcaagag ccccgacagc acgcgccctc cgtcgagcgg cggaatcggg atgaaattga
      841 agaccgctag tatcacattg atcgtgattc caaacacgca catcccgagc agccattgct
      901 cgacacccgt ggtgcccccg agcaagttga gctgcagggc cgcgaagagc gcccaggcgg
      961 tggccatgag gaagttcgag atcggaccgg ccgcggcgac gagaaccatg tcccggcgcg
     1021 ggttgcgcag gttgttgaac gcgacgggca caggcttagc ccagccgaac gcccacgccc
     1081 cgccgctcat gacgagcatc gcgattggca cgatgaccgt gcctagcgga tcgatgtgct
     1141 tcaaggggtt gagggtgagc cggccgagca tgtaggccgt ggggtcgcca aagcggcggg
     1201 cgacccaccc atgggacacc tcgtgaacgg taatggcgaa cagccccggg atcagggcca
     1261 ccgagagaac acggagaatc ttttcgatgt cgatggctgg catcggcgca ttatacgtat
     1321 gtcccggcgc ggcgcaggtt tagctcacga agggggtcac gtcgcccttc ccctgtcgca
     1381 gcacgctgac ctcgtcccgg ctcaggtcga gcaccgaggt cggctcgacg ccgcccgcac
     1441 ccccatcgac gattgcatcg acgagcttgc ccagacggtc gcggatctcc tccgggtcgg
     1501 tgagcggcag aggatccccc ggcagaatga gggtcgagct catgatgggc tccccgagct
     1561 cctcgatgag gcactgggcg atcttgttgt cgggcacccg gatgccgatg gagcggcgct
     1621 ttgggtcctg aagccgcttc ggaacttcgt gcgtggcgcg caggacgaac gtatacggcc
     1681 ccggcgtgag cgagcggagc agccggtagg cccagttgtc cacccgcgcg tacgtggcga
     1741 tctcggcaag gtcccggcac accagcgtga actcgtggtc gcgctcggtc cggcgcaggc
     1801 ggcgcacgcg ctccaggggc tccttggccc cgagccgcca gccgaaggcg tagcaggagt
     1861 ccgtgggata gacgatgacc cccccgtccc gcatgatctc ggcggcccgc tgaagcagcc
     1921 ggcgctgcgg gttcaagggg tgcactttga aatactgagc catgggtcca ttgtaggcgc
     1981 tcgcttcggc ccggcggcgc ccaggcgcta agatggcatg gcgagcgagg ctcaccacat
     2041 gcagctgctg tacgatttct tccccgttct ggtctttttc gtcgcctact acctgggtgg
     2101 catctacgtc gccaccgccg ccatcatcgt ggccatggcg gtccagatcg ccgtcgagta
     2161 cctccgcaag cgcaaggtca gcaagatgct gctcgtgtcc ggcggcctgg tgctggtggc
     2221 cggcgcgctc acgctcgcgc tccgcaaccc tctgttcgtg cagtggaaac ccacgatcgt
     2281 caactggctg ttcgcggccg cgttcctggg tacccacttc atcggcgaca agactctcgt
     2341 cgaacgggtg atgggccacg ccatcgagct cgagcagccg ctctggcggc agctcaatct
     2401 gatgtgggtg gccaactttc tgtttctggg cgccgcaaat ctttatgtcg tctacaactt
     2461 cagcgaagag gcgtgggtga acttcaagct gttcggcatg ctggggttca cgttgctcat
     2521 ggccgtgggc caggcgatct ggatctccgc gaagacggcg caggcgaagc aggaggaaag
     2581 ctaggcccat gctgtatgcc ttcatctgcc gcgacgtgcc gaacagcctg ccgctgcgcc
     2641 aggctacccg cccccgacac ctggagtacg tgcgccccct cgtcgagcag ggccgcatgg
     2701 tcttcgcggg cccgcacccg gccatcgact ccccggatcc cggtcccgcc ggcttcacgg
     2761 gaagcctgat catcgccgag ttcgaatctc tcgaggcggc caaggcgtgg tccgcgagcg
     2821 acccgtacgt gctcgagggc gtgttcgccg aggtggaaat caaaccggtg ctccaaacgc
     2881 tgccgtgacc accgaccgtg tgcagctgat tcgcgaacgc ctcacagcgg cgttcgagcc
     2941 cgaaattctg gatgtgcggg atgacagcca cctgcacgcc gggcacctcg gcgcacgcga
     3001 aggcaagggg cacttcgccg tgaccatcgt gtcgcgccgt ttccagggga agagcattct
     3061 cgagtgtcat cgcatggtat acgaggcgct cggcgacttg atgctcaccg atattcacgc
     3121 gctccaggtg gtcgcgcgcg gtgcgccgta ggcctggacc gacacggacc aaggaggctg
     3181 tgccgatgag agggctgctg ctagcgtccg ccgtggcgct ttttgcgtcg atgctgggct
     3241 gcacgcagcg cgaggcgccc ggcgaggtga accccgcgct cggtctcggc cgagtcgcga
     3301 cagtcaacgg cgaaccgctc cccgagtcga tattcctcta ctacgt
//
LOCUS       DATMBB010000318         4999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_370824, whole genome shotgun sequence.
ACCESSION   DATMBB010000318 DATMBB010000000
VERSION     DATMBB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370824"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VNL92_06850"
     CDS             <1..1483
                     /locus_tag="VNL92_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94466.1"
                     /translation="FLSQIPASFVYSRLLRFQPQSDPEHWNDSVPEGDAAESWEATPD
                     ALTWTFHLRQGMTWHDKPGVGGRPLDAEDVSISLERFRALSPNKANLDMIERVETPDP
                     NTVVMTLSFAYAPFETIMANPNYLWITPKEANAGSVDLTQTMIGTGPFVFDSYEPSVA
                     MRFSKNPTFYEQGIPYVDGVELFIIKDPSTMLSQFISGQLDTHAMTNPRDVEEAKRQI
                     SGLQSDEFLQTLLLFVYMQPYGDANEFYDPNSPFKDERVRRAVSMALDRDAMLQVLYQ
                     GKGRWNQVVPAGFTRFHLNPKDDAGEYGDAAQYYRHDPEAVRQLLAEAGFEDGIDADL
                     HFTNNAYGDLFNQSAEVVNNMLNEAGIRTNLVAEDYGSQYISGTFLGNFQGIAYGPQS
                     GFQEVDEYIFNMLHPSGLRNHSGVNDPELTRMIEAQRVELDAERRREIIHDIQRLVAD
                     KMYYVPTIVGSAEYGRQAYVRNFQFGSFYGIGTETYARVWLDR"
     gene            1967..3142
                     /locus_tag="VNL92_06855"
     CDS             1967..3142
                     /locus_tag="VNL92_06855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWO94467.1"
                     /translation="MRTSEEEEAAVVVPPAFAELGIDEKVLRAIARMGYVTPTPIQHD
                     AIPIVLRGRDLVGQAQTGTGKTLVFAVAIAQAVDATVDETQAIVLTPTRELTQQVAET
                     VRLLAAARGARTVTLYGGHKMGPDFDALRTPPQVVVGTPGRVADHLERGTLRLGAVRI
                     AVLDEADQMLDIGFAPAIDRILSFTPKERQTALFSATLPPFIRRMIRRHLREPEWVRI
                     EPERRTVDEIEQVYFEVSERDKLEGLIELLADADLRRVLIFRRTQRGVDDLARRLERR
                     GVVAAAIHGGLEQSKRERVLAEFRAGRVRALIATNVASRGLDIDDVTHVINYDMPQNL
                     EEYIHRIGRTGRAGRTGLAISFVGEWELAEFEEIAKAFGDKLQRRVLRLYQAAGAAG"
     gene            3299..4186
                     /locus_tag="VNL92_06860"
     CDS             3299..4186
                     /locus_tag="VNL92_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO94468.1"
                     /translation="MPRTILLPFDGSRTSVRALRYAAALARLGDEPAGVLLLTVLPDA
                     EGLEIPEHAGRAEVERAAQRQLASAARRLRKAGIAQVDTLTVWDEDAADAILQVSDDR
                     NVEMIVSGTHGRSGLNRALVGSVALTLMQHADRPTVFVPPGSPLRDVTIRSIVYPVDG
                     STLAEQALPIVEPLAKAGVRVHVVRVVPPSATLVPFAMPGAQGYIPVQVVEDLTAAAE
                     VYVSEFAKRLGPGRAEPHTLIGTPARAITDFANEHKADLIVMTTHGRGAIGRVVMGSV
                     ADQVVRTARMPVLVYRPEQ"
     gene            complement(4200..4487)
                     /locus_tag="VNL92_06865"
     CDS             complement(4200..4487)
                     /locus_tag="VNL92_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWO94469.1"
                     /translation="MSDQELRAIRATVRGRVQGVGFRDFVLRRARTLKLRGWVRNGDD
                     GRSVEVWAEGDEDALQTFRADLARGPALARVDEVEVTPAAPEGASQFQVRY"
     gene            complement(4484..>4999)
                     /locus_tag="VNL92_06870"
     CDS             complement(4484..>4999)
                     /locus_tag="VNL92_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWO94470.1"
                     /translation="MDADLLRLLEEEATRIQELDPSLTTRVIARSVAPKGQVVSEDER
                     ESGRRIILNYGHTVAHGIEAATAYSSYLHGEAVAIGMTAAARISRRMGLLAPEVEARQ
                     RELIEAFHLPTRASGLDPRRVLAAMALDKKVRAKAIRWVLLAGVGMPVVRSDVPDEFV
                     QEAVSYACGEA"
BASE COUNT          889 a         1671 c         1605 g          834 t
ORIGIN      
        1 cttcctcagc cagataccgg ccagctttgt atacagccgg ctgttgcggt tccaaccgca
       61 gagcgacccg gaacactgga acgactccgt cccggagggc gacgccgctg agtcgtggga
      121 ggcgacgccc gacgcgctga cctggacgtt ccacctgcgg caaggcatga cttggcacga
      181 caagccgggc gtgggagggc ggcccctgga tgccgaggac gtttccattt cgctcgagcg
      241 cttccgtgcc ctttcaccga acaaggccaa cctcgacatg atcgagcgcg ttgagacgcc
      301 cgatccaaac acggtcgtga tgacgctgag cttcgcatat gcgccgttcg agacgatcat
      361 ggcgaatccg aactacctct ggattacgcc aaaggaggcg aatgcgggca gcgtcgatct
      421 cacgcaaacg atgatcggca cgggaccgtt cgtcttcgac agctacgagc cgagcgtcgc
      481 catgcgcttc agcaagaacc cgaccttcta cgagcaaggg ataccctacg tcgacggcgt
      541 ggaactcttc atcatcaagg acccttcgac gatgctgtca cagttcatct cggggcagct
      601 cgatacccac gcgatgacca acccgcgcga tgtggaggag gcgaagcgac agatctcagg
      661 gctacaaagc gatgagtttc tgcagacgct gctcctcttc gtctacatgc agccgtacgg
      721 cgacgccaac gagttctacg acccgaactc gccgttcaag gacgaacggg tgcgacgagc
      781 cgtgtcgatg gcgctcgacc gggatgcgat gctccaggtc ctctaccagg gcaaggggcg
      841 ctggaaccag gtggtgcccg cgggattcac gcggttccac ctcaatccaa aggatgacgc
      901 cggcgagtac ggcgatgccg cgcagtacta tcgccacgac cccgaggcgg tgcgacagct
      961 tctcgccgag gcggggttcg aggacggcat cgacgccgac ctgcacttca cgaacaatgc
     1021 ctatggcgac ctgttcaatc agtccgctga ggtcgtgaac aacatgctga acgaagccgg
     1081 catccggacg aatctcgtgg cggaggatta cgggtcgcag tacatcagcg gcacgttcct
     1141 gggcaacttc cagggtatcg cctacgggcc gcagtcaggc ttccaggagg tcgacgagta
     1201 catcttcaac atgctgcacc cctcgggctt gcggaaccac tccggcgtca acgatccaga
     1261 gctgacgcgc atgatcgagg cccagcgtgt tgagctcgat gctgaacggc gccgcgagat
     1321 tatccacgac atccagcgtc tcgtagccga caagatgtac tacgtgccga cgatcgtcgg
     1381 ctctgcggag tacgggcggc aggcgtatgt gaggaacttc cagttcggtt cgttctacgg
     1441 cattggcacg gagacgtatg cgcgggtctg gctcgaccgc tgatcgcaac tcgtcgccct
     1501 tgcctgaaga ggtgcgcccc aagcggcgca cctcttcgca ttgcattcga gcgggtgaac
     1561 ctctagcagt ttcgcccacg atagggtatt ctgttatgtg aatggaacag aacccctgcc
     1621 ccgcacagcc ccacccatcc gacccgatcg ctctccccgc attggctatc caaccaactc
     1681 ccgaaagaat cggaggtcct gtccccgcgt gaaattgtcc ctttttcgcc gcaacggcaa
     1741 atcaaccatc gctacgtcca ccaccacttc gccaacgcca gcagcgcctg ccgcgcctcc
     1801 caccaacgat agagagccaa gcccacgaaa gcgcgcggcc gtaagccccg cagtcgatga
     1861 ggtcggcgta gacgtcgcgc aaccccgccg gcgccgcaat cgcgggcgca agcgagggtc
     1921 cggcgggccc gcatcgccgg cctcgcgggc gggagcgccc gctacgttgc gcacctcaga
     1981 ggaagaggag gccgccgtcg tcgttcctcc cgcgttcgcc gagcttggca ttgatgagaa
     2041 ggtccttcga gcaatcgctc gtatgggcta cgtcactcca acgcccatcc agcatgatgc
     2101 aatccctatc gtattgcgag gccgcgatct cgttggtcaa gcccagacag gcacaggcaa
     2161 gacgctcgtg ttcgccgttg cgatcgccca ggccgtggac gcgacggttg acgagacgca
     2221 ggccatcgtc cttaccccga cccgcgagct cacgcagcag gtcgccgaga ccgtgcgatt
     2281 gctcgcggcg gcgcgaggtg ctcgcacggt gacgctgtac ggcggccaca agatgggccc
     2341 agacttcgac gccctacgaa ctccgccgca ggttgtcgtc gggacccccg gtcgtgttgc
     2401 ggaccatctc gaacggggaa cactgcgcct cggtgcggta cgcatcgccg tgctcgacga
     2461 ggccgatcag atgctcgaca tcggcttcgc cccggcaatc gaccgtatcc tctcgtttac
     2521 gccgaaggaa cggcagacgg cgctcttttc cgcgacgctg cctccgttca tccggcgcat
     2581 gatccggcgg cacctgcgcg agccggaatg ggtgcgcatc gagccggagc ggcgaaccgt
     2641 cgacgagatc gagcaggtgt acttcgaggt ttcggaacgt gacaagctcg aggggctcat
     2701 cgagctcctc gccgacgcag acctgcggcg cgtgctcatc ttccggcgaa cgcagcgcgg
     2761 cgtcgacgat ctggcgcgcc gcttggagcg ccgtggcgtc gtcgccgccg cgattcatgg
     2821 ggggttggag cagagcaagc gggagcgagt gctggccgag ttccgcgccg gccgggtgcg
     2881 agccctcatc gctacgaatg tcgcctcgcg cggcctcgac atcgatgacg tcacgcacgt
     2941 gatcaactac gacatgccgc agaacctcga agagtacatc catcgcatcg ggcgtacggg
     3001 acgcgccggc cgcaccggac tcgccatctc gttcgttggc gaatgggagc tcgcggagtt
     3061 cgaggagatc gcgaaggcct tcggcgacaa gctgcagcgc cgggtgcttc gcctgtacca
     3121 agccgcggga gctgccggct aagggaaact cggcccaatc ccgccgcacg tcgaatcctt
     3181 gcagggtctg tgctcgtgcc tgcgagccgc gcccgctggc cttccactgc cagtggtata
     3241 gtcgggagcg acatccctcg catggtgcga ccgaagaaac gagagaggat agcagcgcat
     3301 gcctcgtacc attctccttc cgttcgatgg gtcccggacg tcggttcgcg cgctgcgata
     3361 cgcagcggcc ctcgctcggc tgggtgacga gcctgctggg gtgcttctgc tcacggtcct
     3421 gcccgatgcg gaaggcctcg agattcccga gcatgccggc cgcgccgagg tagaacgcgc
     3481 cgcgcaacgg cagctcgcct cggctgcgag gcgcctgcgc aaggctggca tcgcccaagt
     3541 cgacacgctg acggtgtggg atgaggacgc tgcggatgcc attctgcagg tctctgacga
     3601 ccgcaacgtg gagatgatcg tcagcggcac gcatgggcgc agcggcctca atcgtgcgct
     3661 tgtcgggagc gtcgcgctaa cgctcatgca gcacgccgat cgtccgacgg tcttcgtccc
     3721 gcccgggtcg cccctgcgcg acgtcacaat ccgaagcatc gtctacccgg tcgatgggtc
     3781 gacgctcgcc gagcaggcgt tgcccatcgt cgagccgcta gcgaaggcgg gcgtacgcgt
     3841 ccatgtcgtg cgcgtggtgc ctccgtccgc gacgctcgtg cccttcgcga tgccgggcgc
     3901 tcagggatac attccggtgc aagtcgtgga ggacctcacc gcggcggccg aagtgtacgt
     3961 gtcggagttc gcgaaacggc tcgggcccgg gcgagcggag ccgcatacgc tcatcggcac
     4021 ccctgctcgc gccatcacgg acttcgcgaa cgagcacaag gcggacctca tcgtgatgac
     4081 gacgcatggc aggggcgcga tcggccgcgt cgtgatgggc agcgttgctg accaggtggt
     4141 tcggacagca cggatgccag tcctcgtgta ccggccggag cagtgagggc cgaactcgtc
     4201 tagtaccgca cctgaaactg ggaggcgccc tccggcgcgg ccggcgtgac ctcgacctca
     4261 tcgacgcggg cgagtgcggg gcctcgtgcg aggtcggccc gaaaggtctg gagcgcgtcc
     4321 tcgtcacctt cggcccagac ctcgacgctc cgaccgtcgt ccccgttgcg aacccaaccg
     4381 cgaagcttca gggtccgagc ccggcgcagc acgaagtcac gaaagccgac gccttgcact
     4441 cggccgcgca cggtggcccg gatggcgcgc agctcttggt cgctcatgcc tcgccgcagg
     4501 cgtaggacac tgcttcttgt acgaactcgt cgggaacgtc gctccgcacg acgggcatgc
     4561 ccacgccggc gagcaggacc caacggatcg cctttgcccg caccttcttg tcgagcgcca
     4621 tggcggccag gacgcgccgc gggtccaagc cggacgcgcg tgtaggcaga tggaacgcct
     4681 cgatgagctc gcgctgccgc gcctccacct cgggtgcgag gaggcccatg cgacggctaa
     4741 tgcgagcggc tgcggtcatg ccaatcgcga cagcctcgcc gtgaagatat gagctgtagg
     4801 ccgtcgccgc ttcgatgccg tgagcgaccg tatgcccgta gttcaggatg atgcgccggc
     4861 ccgattcgcg ttcgtcctcg ctgaccacct gccccttggg ggcgacgctc cgggcaatga
     4921 cgcgagtcgt tagcgagggg tcgagctcct ggatcctcgt cgcctcctcc tcgagcaggc
     4981 ggagcaggtc ggcgtccat
//
LOCUS       DATMBB010000319         5412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_368134, whole genome shotgun sequence.
ACCESSION   DATMBB010000319 DATMBB010000000
VERSION     DATMBB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368134"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VNL92_06875"
     CDS             complement(<1..1032)
                     /locus_tag="VNL92_06875"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWO94471.1"
                     /translation="MDEGYVLLAIILVPLVGALVLMAIPSRFVLATRLFALLASAAMF
                     GLSVYTFVGYDYDAGGIQMAHRWDWLQNIGFLGEDGVSLFLGLDGIGAPMVLLNGIVI
                     FAGAMISWKIDVRPKDFFVLLFVLVAGVYGTFISQDLFFFFFFYEVAVLPMYILIAVW
                     GSTRREYGAMKLTLMLVAASVLIWVGIFAIFHEAGRGTFDLEVLGSVTYDENFQKLFF
                     PIVTIGFGVLAGLWPFHTWSPDGHVAAPTAVSMLHAGVLMKLGAFGILRVSMTLMPEG
                     AEFWMPGLMVLGTINAVYGAMSAFAQRDLKFVIGYSSVSHMGYVLMGLATLNEIGVTG
                     AVLQMFAHGV"
     gene            complement(1078..2979)
                     /gene="nuoL"
                     /locus_tag="VNL92_06880"
     CDS             complement(1078..2979)
                     /gene="nuoL"
                     /locus_tag="VNL92_06880"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWO94472.1"
                     /translation="MLDLDLAWLIPVVPGGAFIVLLLLRNLLPRGGDFVAIGAIFASL
                     VLFVLLGIEFSDRLADGTGIPARNGFDWVDFGDFHIRIGFYVDEIAIVMLLVVTVVAL
                     MVNVYSVGYMHGDKRYGWYYTVLALFAFSMLTLVLADNFLMLYLTWELVGLCSFLLIG
                     FWYERRSAAEAAKKAFVTTRIGDVGLLIGIILFWRELGTFDFQAIIQAVEAGEIDDPM
                     LTIATLLVFAGAVGKSAQVPLHVWLPDAMEGPTPVSALIHAATMVVAGVYLVARTLPI
                     FLAAPGAAEVVTGIGLTTALLAATMGLVTTDIKRVLAYSTVSQLGFMFVALGVGSVTA
                     AMFHLMTHAFFKALLFLGSGSVIHATDRQDVDQLGGLWRKMPITTVTFGIATLALAGI
                     PPLAGFWSKDEILLAVQHESNAAVFLLLGFATFLSALYMTRLFVLTFLGEPKDRHAYE
                     HAHESPPPMALPLVILAILTLVAGFVALSGVGEALGFPGGFGEVVFLEEAEEWSWHGD
                     LAIGATLLGVAGVALGVYIWLLEGGRRAKAIGDWAPNIYALLANRYYIDDLYQAGINY
                     AMVGGARIIAWFDRNIVNDTGVDGSSNLTVYLGDRLKYIETGKLPNYALGIVIGIVAL
                     ALAAFATQT"
     gene            complement(3001..3309)
                     /gene="nuoK"
                     /locus_tag="VNL92_06885"
     CDS             complement(3001..3309)
                     /gene="nuoK"
                     /locus_tag="VNL92_06885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWO94473.1"
                     /translation="MIPLEQYLVLSGILFSLGVFGVLVRRNAVLIIMSVELMLNAVSI
                     NLIAFAGYLSPDEFSGLIFAVFVITIAAAEAGLALGLVMYLYRQRRTVNVDEADTLRW
                     "
     gene            complement(3310..3801)
                     /locus_tag="VNL92_06890"
     CDS             complement(3310..3801)
                     /locus_tag="VNL92_06890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWO94474.1"
                     /translation="MEQVVFYILAALAVAGGLGVVLSQNVARAALSLILTLAMVAAIF
                     LLLAAEFLALVQILIYGGAVTILLLFALMLTRARELPRAGFGAQWPFAIVGAGGLLAV
                     IVMMVADTDWHAPDEPTAIGFEEFGDTLFRVWSVPFEIASLVLLVALVGAILLAGRDE
                     EES"
     gene            complement(3818..4279)
                     /locus_tag="VNL92_06895"
     CDS             complement(3818..4279)
                     /locus_tag="VNL92_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWO94475.1"
                     /translation="MIGVLKGLATTFLTFTRKPVTVHYPDVHRTLPERNRGFPVLLWD
                     AAVPEPFCTGCHACERACPVECMTVVMKDNPRHATGESKRRKIVDKFYIDYARCMRCN
                     ICVEVCNFEAIALNNTWKTHEMSSYDRSDLVLDLEDLLAQSRAGDIVPFTG"
     gene            complement(4276..>5412)
                     /gene="nuoH"
                     /locus_tag="VNL92_06900"
     CDS             complement(4276..>5412)
                     /gene="nuoH"
                     /locus_tag="VNL92_06900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWO94476.1"
                     /translation="RTVFGGPMTDDFWFDMPWLDALLRILIVVVAMTLAVLALIYLER
                     KFIGRLQNRVGPTRTGPMGLFQSAADALKLVGKEDLRPATADRWVFELAPYVVFVPTF
                     LGLMALPFTYDVVVRHMELALFYIVAITSLSTVGMIMAGWGSDSKYPLLGAARTAALL
                     IGYELPLVLSILSITMFADTLDIMAIVRQQDTVPFIVWQPLAFVLFMFAATAEIHRPP
                     FDMPVAESEIVGGPLVEYSGIRWGMFFLAEYVSLVIAAVLASTLFLGGWEWPFGEDAG
                     GWLQGILIFVKTSLFIAFFFWIRATLPRLRIDQMTAFCWKVLLPFSLLQILLTGLVMV
                     YDWPDVVLLVLSGIALAAMLAVIYSSVQRPTAEQRAILRRRLAI"
BASE COUNT         1187 a         1625 c         1772 g          828 t
ORIGIN      
        1 cacgccatgg gcgaacatct gtaacacggc gcccgtcacg ccgatttcgt tgagcgtcgc
       61 gaggcccatg agcacgtagc ccatgtgact cacgctcgaa tagccgatga cgaacttgag
      121 atcgcgctga gcgaaggccg acatggctcc gtacacggcg ttgatcgtcc cgagcaccat
      181 gagcccaggc atccagaatt cggcgccttc cggcatcagt gtcatcgaga cccgcagaat
      241 gccgaacgcg ccgagcttca tgaggacgcc ggcgtggagc atggacacgg ctgtcggtgc
      301 cgcgacgtga ccatccggcg accacgtatg gaatggccac aggcccgcga ggacgccgaa
      361 gccgatggtc acgatgggga agaagagctt ctggaagttc tcatcgtagg tcacgctgcc
      421 gagcacctcg aggtcgaacg ttcctcgacc ggcttcgtgg aagatggcga agatgccgac
      481 ccaaatgagt acgctcgccg ccacgagcat cagggtcagc ttcattgcgc cgtactcgcg
      541 gcgcgtgctg ccccaaacgg cgatgaggat gtacatgggg aggacggcaa cttcgtagaa
      601 gaagaagaag aagaacaggt cctggctaat gaaggtgccg tagacgccgg caaccaggac
      661 gaacaagagg acgaagaaat ccttggggcg gacatcgatc ttccacgaga tcatcgcgcc
      721 cgcgaagatg acgatgccgt tcagcagcac catcggcgcc ccgatgccgt cgaggccgag
      781 aaagagcgag acgccgtcct cgccgaggaa tccgatgttc tgcagccagt cccagcggtg
      841 agccatctgg atgccgccgg cgtcgtagtc gtacccgacg aacgtataga ccgagaggcc
      901 gaacatggct gcgcttgcca gcagggcgaa gaggcgcgtg gccagaacga agcgcgacgg
      961 gatcgccatc aacacgagcg cgccaacgag cggcaccagg atgatggcga gaaggacata
     1021 gccttcgtcc aagggcgtct cctttggcgc tcctaaggcg agcggctcgg agcagcgtta
     1081 ggtctgcgtc gcgaaggccg ccaatgccag ggcgacgatg ccgataacga tgcccagagc
     1141 gtagttcggc agcttgccgg tctcgatgta cttcagtcga tcgccaaggt agacggtgag
     1201 attgctcgag ccgtccacgc ccgtatcgtt cacgatgttc cggtcgaacc aggcgatgat
     1261 gcgagcaccc ccgaccatcg cataattgat gcccgcttga tacaggtcat cgatgtagta
     1321 gcgattcgcg aggagtgcgt agatgttcgg cgcccaatcg ccgattgcct tggcccgacg
     1381 cccgccttcc agcagccaga tgtacacgcc gagggcaaca cccgcgacgc ccaggagcgt
     1441 ggccccgatg gcgagatccc cgtgccacga ccactcctcg gcctcctcga ggaagaccac
     1501 ctcgccgaag cctcccggga agcccagcgc ctcgcccacg cccgagagcg caacgaaccc
     1561 agcgacgagc gtaaggatgg cgagtatcac gagcgggagc gccatgggcg gcggcgattc
     1621 gtgggcatgc tcataggcgt gacggtcctt cggctcgccg aggaaggtca acacgaagag
     1681 gcgcgtcatg tacagggccg acaggaacgt cgcgaatccg aggaggagaa acaccgcggc
     1741 gttgctctcg tgttggacag cgagcagaat ctcgtccttg gaccagaacc cggccagcgg
     1801 tgggatgccg gcgagagcca aggtcgcgat gccgaatgtg accgtcgtga tgggcatctt
     1861 ccgccacagt ccgccgagtt gatccacgtc ctgacggtcg gtggcgtgga tgacggaacc
     1921 cgagccgagg aagagcagtg ccttgaagaa cgcgtgcgtc atcaagtgaa acatggcggc
     1981 ggtaaccgac ccgacgccga gcgcgacgaa catgaacccc agctggctaa cggtcgaata
     2041 ggcgagcact cgcttgatgt ccgtggttac aaggcccatc gtcgccgcga gcaatgcggt
     2101 tgtgaggccg atgccggtga cgacttcggc ggcgcccggg gcggccagga agatcgggag
     2161 cgttcgtgcg acgaggtaga ccccggccac gaccatcgtg gccgcgtgga tgagcgctga
     2221 aaccggcgtt ggaccttcca tcgcgtcggg gagccagacg tgtagcggaa cctgcgctga
     2281 cttgcccacc gcacccgcga acacgagcag tgtcgcgatc gtgagcatcg gatcatcgat
     2341 ctcgccggcc tcgaccgctt ggatgatcgc ttggaagtcg aaggtcccta gttcgcgcca
     2401 gaacaggatg atgccgatga gcagaccaac atcgccgatg cgcgtcgtga cgaacgcctt
     2461 cttcgcggcc tccgcggcgc tccgtcgctc gtaccagaag ccgatgagca ggaacgagca
     2521 gaggccgacg agctcccacg taaggtagag catgaggaag ttgtccgcca gcacgagggt
     2581 gagcatggag aaggcgaaca gcgcgaggac ggtgtagtac cagccatagc gcttgtcgcc
     2641 gtgcatgtat cccaccgagt agacattgac catcagggcg acgaccgtga cgacaaggag
     2701 catcacgatg gcgatctcgt cgacgtagaa gccgatacga atgtggaaat cgccgaagtc
     2761 gacccagtcg aagccgttgc gagccggaat accggtgccg tcggctagcc ggtcggagaa
     2821 ctcgatgcca agaagcacaa acaggacgag cgaggcgaag atcgcgccga tggcgacgaa
     2881 atcgccgcca cgggggagaa ggttccgaag gaggagcagc acgatgaagg cgccgcccgg
     2941 tacgaccgga atcagccacg cgaggtcgag gtcgagcatc ggtctggccg cgctcctagc
     3001 tcaccagcgg agggtgtctg cttcgtcgac gttgacggtc cggcgttgac ggtacaagta
     3061 catgacgagc cccagggcca gtccggcttc ggctgcggca atggtgatga cgaacaccgc
     3121 gaagatgagc cccgagaact cgtccggcga caagtatcct gcgaacgcga tgaggttgat
     3181 gctgacggca ttcagcatga gctcgaccga catgatgatg agcacggcgt tccttcgtac
     3241 cagtacgcca aagacgccga gtgagaacag gatgccgctg agtacgaggt actgctcgag
     3301 cgggatcatc tacgactcct cctcatcccg gccggcgagc aggatggcgc cgaccagggc
     3361 gacgagcaga acgagcgacg caatttcgaa ggggacggac cacacgcgga acagcgtatc
     3421 gccgaactct tcgaacccta ttgccgtggg ttcatcgggc gcatgccagt ccgtgtccgc
     3481 caccatcatg acgatgacgg caagcaggcc gccggcgccg acgatggcga atggccactg
     3541 ggcgccgaat cccgctctgg gcagctccct tgcccgggtg agcatgagcg cgaagagcaa
     3601 caggatcgtc accgcgccgc catagatgag tatctggacg agggcgagaa actcggcggc
     3661 gaggaggagg aagatcgctg ccaccatcgc cagggtcagg atcagcgaga gcgccgctcg
     3721 ggccacgttc tgagagagaa cgacgcccaa gccgccggcg acggcaagtg cggccaggat
     3781 gtagaagact acttgctcca ttcgctcccg atcggtctca gcccgtgaag ggcacgatat
     3841 cgccggccct cgattgggcg agcaggtcct ccaggtccag taccaggtcc gatcggtcgt
     3901 agctggacat ctcgtgggtc ttccacgtgt tgttcagcgc gatagcttcg aagttgcaca
     3961 cctcgacgca gatgttgcac cgcatgcagc gcgcgtagtc gatgtagaac ttatcgacga
     4021 tcttgcgtcg cttgctttcg ccggttgcgt gacgcgggtt gtccttcatc actaccgtca
     4081 tgcattccac cgggcacgcg cgctcgcagg cgtggcaccc ggtgcagaac ggctcgggca
     4141 ccgcggcatc ccaaagcagg acgggaaagc cgcggttgcg ctccggcaag gtgcgatgca
     4201 cgtcggggta gtgaacggtt acgggctttc gcgtgaacgt gagaaacgtt gtcgccagtc
     4261 ccttcagcac gccaatcata tcgcgagtct ccttcgaagg atggcccgtt gctccgcggt
     4321 cgggcgctgc acgctgctgt aaatgacggc gagcattgcg gcgagagcta tgcccgagag
     4381 cacgaggagc acaacgtcgg gccaatcgta caccatgaca agccccgtga gcaggatttg
     4441 gagcaacgag aacggtagca gcaccttcca gcagaaggcc gtcatctggt cgatgcgaag
     4501 gcgaggcagc gttgccctga tccagaagaa gaaggcaatg aagaggctcg tcttcacaaa
     4561 gatgaggatg ccctgaagcc agccgcccgc gtcctcaccg aacggccact cccagccacc
     4621 aagaaagagc gtcgatgcga gcacggccgc gatgacgagc gagacgtact ccgcgaggaa
     4681 gaacatgccc caccggatgc cactgtattc aacgagcggt cccccgacga tctccgattc
     4741 ggcgacgggc atgtcgaacg gcggccggtg aatctcggcg gtcgcggcga acatgaagag
     4801 cacgaacgcc agcggttgcc agacgatgaa tggcaccgtg tcctgctgtc ggacgatcgc
     4861 catgatgtcg agcgtgtcgg caaacatcgt gatcgagagg atggacagga cgaggggaag
     4921 ctcgtacccg atgaggaggg cggcggttcg tgccgcaccg aggaggggat acttcgagtc
     4981 ggacccccac ccggccatga tcatcccgac ggtcgacaag ctcgtgatgg cgacaatgta
     5041 gaagagcgcc aactccatgt ggcgtacgac gacgtcgtag gtgaatggta gggccatcag
     5101 gcccaggaag gtcggcacga acacgacgta cggtgccaac tcgaacaccc accggtcggc
     5161 ggtggctgga cgcaagtcct ccttgccgac gagtttcagg gcgtcggcag cagattggaa
     5221 caggcccatc gggcccgtgc gcgtcggtcc gacgcggttc tgcaggcgac cgataaactt
     5281 ccgctcgagg tagatgaggg cgaggaccgc cagggtcatg gcgaccacga caataaggat
     5341 gcgcagcagg gcatcgagcc acggcatatc gaaccagaaa tcgtcagtca tcggtccacc
     5401 gaatacagta cg
//
LOCUS       DATMBB010000320         3378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_539810, whole genome shotgun sequence.
ACCESSION   DATMBB010000320 DATMBB010000000
VERSION     DATMBB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539810"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VNL92_06905"
     CDS             <1..841
                     /locus_tag="VNL92_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWO94477.1"
                     /translation="EATARGVADATAAGCTAIIIDTAGRSHVDEEMMREIIAIRDAVQ
                     PHESLLVVDAMTGQEAVNAAAEFHERVGLTGFILTKLDGDARGGAALSIRSVTGLPVK
                     FVGAGEKIEALEPFYPDRFASRILGMGDVLGLVERAQQNIGEDGIGRLEEKMKSRSFD
                     MQDFLEQYQSLRKMGSLQQLLAMVPGLSLVKSRIKVDDIDDAFLTKVEAMILSMTPDE
                     RRNPAMINGSRRKRIARGSGTTPQDVNQLLSQFREVQKIMQMMTSGKFSLVPGLKFPG
                     VRR"
     gene            858..1118
                     /gene="rpsP"
                     /locus_tag="VNL92_06910"
     CDS             858..1118
                     /gene="rpsP"
                     /locus_tag="VNL92_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWO94478.1"
                     /translation="MVRIRLRRQGKKRQPIYRIVVADSRAPRDGDFIETIGLYNPLTD
                     PETVTVDEERLRHWLGHGAQPSETVDKLLRRKGFLPTAAASS"
     gene            1118..1384
                     /locus_tag="VNL92_06915"
     CDS             1118..1384
                     /locus_tag="VNL92_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWO94479.1"
                     /translation="MVTQAPEPPPYADLIEYIARALAEKQDEVAVSEEWDEDHCTVRL
                     RVSEDDMGRVIGRAGRVAQAMRTLLRVAAIRSGDRATLEIVESD"
     gene            1394..1978
                     /gene="rimM"
                     /locus_tag="VNL92_06920"
     CDS             1394..1978
                     /gene="rimM"
                     /locus_tag="VNL92_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWO94480.1"
                     /translation="MAPKKQGPAKTPPGRSPRVQEPSHGRVAVGRVLRPWGTQGELKV
                     EPLTDFVRERFAPGARLWAGANRHTIAGYREHQGQSLVRFEDVRDRNAAEELRGELLE
                     VAEEELASLPPDTYYRHDLLGLAVITTGGERLGEVTEVLDTGANEVFVVAGEGWEILV
                     PAIGDVVTDVDVPGGRIVIEPTPGLLPEERGDKS"
     gene            complement(2031..2552)
                     /locus_tag="VNL92_06925"
     CDS             complement(2031..2552)
                     /locus_tag="VNL92_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWO94481.1"
                     /translation="MRQRSNDVADLTPSERALARIAALSRGEEELDLAALLRLPYQLL
                     VERLHEDLAARGFPDIRVTHGKNILAHVDKRGVRIVDLAEAAQLTKQSTAELVLYLEQ
                     RGYVTRTDDPLDGRAKLIHLTDRGVAAHGMASTIIQEISDEWAECMGPDSFERLLVLL
                     RQLIRSLLRERGD"
     gene            2686..>3378
                     /locus_tag="VNL92_06930"
     CDS             2686..>3378
                     /locus_tag="VNL92_06930"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWO94482.1"
                     /translation="MVLYTSGPSVGTEPYIEETDEDFRVSPRIFTDPAIFEEEMERIF
                     GRAWVYVGHVSEIAQAGDYKTSSIGRTPIILSRDQHGQIHVLVNACRHRANAVCRSDA
                     GNSTYFRCPYHGWVYANDGRLIGVAMRKGYPPSFGEDVDGLVRAARVAVYRGLIFASM
                     APAGPTIEEYLGPVARHIDAWADQAIEGDLEVVRPHQYNYFGNWKFQAENGADGYHPG
                     FVHESAFKTFSKF"
BASE COUNT          651 a         1028 c         1107 g          592 t
ORIGIN      
        1 agaggcgacg gcgcggggag tggccgacgc taccgcggcg ggctgtaccg cgatcatcat
       61 cgatacggcg ggccgctcgc acgtcgacga ggagatgatg cgcgagatca tcgccatccg
      121 tgatgcagtg cagccgcacg agtcgctcct tgtcgtcgac gcgatgaccg gacaggaggc
      181 cgtgaatgcc gcggcggagt tccacgagcg cgtaggactc accggattca tcctgaccaa
      241 gctcgatggc gacgcacgcg gtggcgccgc gctctcgatc cgctccgtga cgggcctccc
      301 ggtcaagttc gtcggagccg gcgagaagat cgaggcgctc gagcccttct acccggaccg
      361 cttcgcgtcc cgcatcctcg gcatgggcga tgtcctcggt ctggtcgagc gggcgcagca
      421 gaacatcggc gaggacggca ttgggcggct cgaagagaag atgaagagcc gcagcttcga
      481 tatgcaggac tttctcgagc agtaccagag cctgcgaaag atgggatcgc tacagcagct
      541 gctggctatg gttccaggtc tttccctcgt caagagtcgc atcaaggtgg acgacatcga
      601 cgacgccttc ctgacgaagg tggaggcgat gatcctgtcg atgacgccgg acgagcgacg
      661 caatccggcc atgatcaacg ggagccggcg caagcgcatt gcccgtggaa gcggaaccac
      721 cccgcaggac gtcaatcagc tcctcagtca gttccgcgaa gtgcaaaaga tcatgcagat
      781 gatgacctcg ggcaagttta gcctggtgcc cggactcaag ttccccggcg ttcgccgcta
      841 accatcggga ggattacgtg gttcgaattc gcctgcgtcg acaaggcaag aagagacagc
      901 ccatctatcg catcgtcgtc gccgactcgc gtgcgccgcg ggacggcgac tttatcgaga
      961 ccatcggcct ctacaatcca ctgaccgacc cggaaacggt gaccgtcgat gaggagcgac
     1021 tgcgccactg gttgggccac ggagcgcagc cttccgaaac ggtcgataag ctcttgaggc
     1081 gcaaggggtt cttgcccaca gccgctgcct cgtcctgatg gtaacccagg ccccggaacc
     1141 gcccccctac gctgatctca tcgagtacat cgcgcgcgcc ctcgctgaga agcaggacga
     1201 ggtcgcggtc agcgaggagt gggacgagga ccactgcacg gtccgcctcc gggtgtccga
     1261 ggatgacatg ggacgggtga ttggacgcgc ggggcgggtc gctcaggcaa tgcggaccct
     1321 gcttcgcgtc gctgccattc gcagcggcga ccgtgcgacg ctcgagatcg tggaatcgga
     1381 ctgaccggcg gatatggcgc cgaagaagca gggtccggcg aagactccgc ctggccgaag
     1441 ccctcgcgtc caggagccct cacacggccg cgtcgccgtt gggcgcgtcc ttcggccttg
     1501 gggtacgcaa ggcgagctga aagtggagcc gctgacggac ttcgtccgcg agcggttcgc
     1561 ccctggggct cgattgtggg ccggcgccaa tcgccacacc atcgccgggt atcgcgagca
     1621 tcaaggccag agcctggttc gcttcgagga cgtacgcgac cgcaacgccg ctgaggagct
     1681 acgcggcgag ctgctggagg ttgcggagga ggagctggcc tccctgccgc cggacacgta
     1741 ttaccgtcac gatctgctgg gtctcgccgt catcacgaca ggcggggaac gcctcggcga
     1801 ggtcacggag gtcctcgata cgggggcgaa cgaggtgttc gtcgtcgctg gggaggggtg
     1861 ggagatactc gtaccagcca tcggagacgt ggtcacggac gtcgacgtgc ccggcggccg
     1921 catcgtcatc gagccaaccc ctggcctgct acccgaggag cgtggtgaca agtcgtgagg
     1981 ggaggggact ggtgctgggg ggcggagtcg aaccgcataa gccggtatcg ttagtcgccc
     2041 cgttctctta gaagggaccg aatgagctgg cgaagcagaa ccagcaagcg ctcaaaggag
     2101 tcgggaccca tgcactcagc ccactcatcc gagatctcct gaataatcgt cgaggccatc
     2161 ccgtgtgcgg cgacacctcg gtcggtgagg tgaatgagct tggcgcgtcc atcgagtgga
     2221 tcgtcagttc gcgtgacgta accgcgctgc tcgagataca ggacgagctc cgccgtcgac
     2281 tgcttggtca gctgcgccgc ctcagcgagg tcgacgatac gcacgccacg cttgtcgacg
     2341 tgggccagaa tgttctttcc gtgcgtgacc cggatgtctg ggaacccccg cgcggcaagg
     2401 tcctcatgca atcgttccac gagcagttga tacgggagtc gaaggagggc cgcgaggtcc
     2461 agctcttctt cgcctctcga tagcgccgcg atgcgcgcga gcgcacgttc gctcggggtc
     2521 aggtccgcga cgtcattaga gcgctgtctc ataagacagc tcctcctagc tagctagaac
     2581 gatcctccgt tcacaagcac gtaacaggcc tgcaatggac tggtaacctg actatcgcac
     2641 catacagaca tcgagtcgcc cttctggata gacggaggtt agcacatggt gctctacacg
     2701 tcaggcccat cggtaggcac ggagccctac atcgaggaga cggatgagga cttccgggta
     2761 agcccacgca tctttacgga tccggccata ttcgaggaag agatggagcg catctttggt
     2821 cgggcgtggg tatacgtagg acacgtcagc gagatcgctc aggccggaga ttacaagacg
     2881 agttccatcg gccgaactcc catcatcctg agccgtgacc agcacggaca gatccacgtg
     2941 ctcgtcaatg cttgtcgtca ccgggcaaac gccgtttgcc gttcggatgc gggtaactcc
     3001 acgtactttc gctgtcccta ccacggttgg gtttatgcga atgacggtcg tctgatcggt
     3061 gtcgccatgc gaaagggcta tcctccgagc tttggcgagg acgtggacgg actagtgaga
     3121 gccgcccggg tcgcggtcta ccgcgggctc attttcgcga gcatggcgcc ggcgggtcca
     3181 actatcgagg agtacctcgg acctgtcgcc cgtcatatcg acgcatgggc cgaccaggcg
     3241 attgagggcg acctggaggt cgtgaggccc caccaataca actacttcgg aaactggaag
     3301 ttccaggccg agaatggggc cgatggctat caccccggct ttgtccacga atccgcattc
     3361 aagacgttct cgaagttt
//
LOCUS       DATMBB010000321         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_255426, whole genome shotgun sequence.
ACCESSION   DATMBB010000321 DATMBB010000000
VERSION     DATMBB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255426"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..1030
                     /locus_tag="VNL92_06935"
     CDS             485..1030
                     /locus_tag="VNL92_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWO94483.1"
                     /translation="MVTDLGGRLRALRRQRGLSIADVSAATGISTSFLSLIENGKSDI
                     TTTRLLRLVSFYKVHITDLLPQPEASEQIVVRASEAVRLSSASEGIETLLLVANTNRA
                     MLPVIQYYDPGAGTQEYAQHEGEEFLHVLQGSIALDLNGTEIVLKKGDSAYFAADQPH
                     MYRNAGRGKVTILAVVTPPTI"
     gene            1044..1808
                     /locus_tag="VNL92_06940"
     CDS             1044..1808
                     /locus_tag="VNL92_06940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO94484.1"
                     /translation="MYEHTRLLRLDEKTAIVTGASSGIGQAIAEAMAAAGARVLLAGR
                     DVQRLTETYAGIRAAGGTCVWRAFDLTVPGACTGLVEAALESFGAIDSLVHAAGLFWP
                     QPFHETPLDDLDRQWEVNVRVPFELTQAALPHLRDGASVIFISSIAGHIGFPRSSAYC
                     ATKGAVELLTKALAVELAPRGIRVNAIAPGNIRTPMNAHLLADEDYLKAMLDATPARR
                     IGTVSEIAPFAVFLASDAASFVHGSSFLIDGGWTAT"
     gene            2287..>2554
                     /locus_tag="VNL92_06945"
     CDS             2287..>2554
                     /locus_tag="VNL92_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO94485.1"
                     /translation="MTRAVEEAGLRLYKQGRVPGSFYDGRGQEATAVGAALAMEERDV
                     ACPLIRDMGVHLVRGVTPAEMLRHFLGKAGGPMEGRDGNIHLGSL"
BASE COUNT          451 a          871 c          806 g          426 t
ORIGIN      
        1 ccgcgtcgcc gcgtgctgcg gccgcttgca gcggctggca tgcctcgtcg atctctgaca
       61 gctcgccgcc catggcgagc gccgtcataa ccgacagcga ccagccgtag ttctcaggga
      121 agtactcgaa cacccgtggg ctccttgtgc gagaggacgt cggttcgccg gtcaagcccg
      181 gcctggagca gtcgacctag cggatgttcg gcgcgcgagc ccagtcctgg ctcacggtgc
      241 gccagcgcgc gaagacgaag atcgggccgg gcaccggtcc aatcagtgtc aggactcggc
      301 cgcgtctgat cgtgccggtc cctggacgcc tgcgccgctc gtgaggatct cgacccactg
      361 aaggttcgcc agtatacaca aaaaacattt gacatactgg atagccatgc cacgcgtttc
      421 aggacccgca ggggcagcct ccaacgcctc gccagccgca ggctccggtt cgggcgagaa
      481 cagggtggtc accgacctcg gtggccgcct tcgagccctc cgtcgccagc gtggcttgtc
      541 gatcgccgac gtatcagccg ccaccggcat ctcgacctcc ttcctctcgc tcatcgagaa
      601 cggaaagagc gacatcacga ccacgagact gctgcggctc gtcagcttct acaaggtgca
      661 catcaccgac ctcctgccac agcccgaggc ttcagagcag atcgtcgtcc gcgccagcga
      721 ggccgtccgg ctctcctcgg cctccgaggg aatcgagacg ctcctcctcg tcgcgaacac
      781 gaaccgagcg atgcttcccg tgatccagta ctacgacccc ggtgcaggca cccaggagta
      841 cgcccagcac gagggcgagg agttccttca cgtcctccag gggtcgatcg ccctcgatct
      901 gaacggaacc gagatcgtcc tcaagaaggg cgacagcgct tacttcgcag ccgaccagcc
      961 gcacatgtac cgaaacgccg gacggggcaa agtgaccatc ctcgccgtag tcacgccacc
     1021 aacgatctga ggagagaagc tccatgtacg agcacacacg cctgctgcgc ttggacgaaa
     1081 agacggcaat cgtcaccggc gcgagctccg gcatcggaca ggcaatcgcc gaggcgatgg
     1141 ccgcggccgg agccagggtt cttctggcgg gccgtgacgt gcagcgtctg acagaaacct
     1201 acgcgggtat tcgcgcggcc ggtgggacct gcgtgtggcg tgctttcgac ctcacggtgc
     1261 ccggcgcctg cacggggctc gtcgaggccg ccctcgagtc cttcggggcg atcgacagcc
     1321 tcgttcacgc cgctggtctc ttctggccgc agccatttca cgagaccccc ttggacgacc
     1381 tcgaccgaca gtgggaagtc aacgtgcgcg tcccgttcga gctgactcaa gcggccctac
     1441 cgcacctacg cgacggtgct tcggtcatct tcatctcctc catcgccggt cacatcgggt
     1501 ttccgcgctc ctccgcctac tgcgcgacga agggagccgt cgagcttctc acgaaggccc
     1561 tcgccgtcga gctggcccct cgaggcatcc gtgtcaacgc aatcgcaccc ggcaacatcc
     1621 ggactccgat gaatgcccac ctcctcgccg acgaggacta cctcaaggcg atgctcgatg
     1681 ccacaccggc aagacggata gggacggtct ccgagatcgc ccccttcgca gtcttcctcg
     1741 cctccgacgc cgccagcttc gtgcacggct cgagcttcct gatcgacgga ggctggacag
     1801 ccacctagag gtgtggcctc tgccaagact ggcccgtttc gcccctacgg cgagacgagg
     1861 tcaagtcgag ggcgcgtcga gttcctcgat gcgatcgaac cgatcgagac gtcggcgtat
     1921 cacgggatcg atcacgacgt acctctgtcg gccccttggc gagggccccc agcgacaggc
     1981 gaagcaggcc ctggtcctcc agacggacgt cgaggagcac gttcgcgtcc tgaggacggc
     2041 tgcggcgccg cgttcagccg ccaaacccag atcgctcggc tcgagcgcac agtcgcagat
     2101 cacggctcag gtgccacgcc cgatagtgag ttatcggatc cgaaataagg ttccccggcg
     2161 cccaaacggg aaaattgcta tcatctcgga tccgagatgg ccaccccggc cgattccctg
     2221 caacgcccga taggagcatc atcggatcgt ggtgcgtttc tgcgcactgc gggctggtgg
     2281 cttcacttga cgcgggcggt cgaggaggcg ggtcttcgcc tgtacaagca agggcgcgtg
     2341 ccgggctcgt tctatgacgg gcgcggtcag gaggcgacgg cggtaggggc ggcgctcgcg
     2401 atggaggagc gtgatgtggc ctgtccgctg attcgtgaca tgggggtgca tctcgtgcgg
     2461 ggggtgacgc ccgccgagat gttgcgacac ttcctgggca aggccggggg gcctatggaa
     2521 ggccgcgacg ggaacatcca tctgggctcg ctgg
//
LOCUS       DATMBB010000322         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_292023, whole genome shotgun sequence.
ACCESSION   DATMBB010000322 DATMBB010000000
VERSION     DATMBB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292023"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VNL92_06950"
     CDS             complement(<1..567)
                     /locus_tag="VNL92_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002692299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWO94486.1"
                     /translation="MQNSTLKLAARATHVIPGGVNSPVRAWKAVGGNPRFVNRGSGAY
                     LWDCEGRRYVDLVCAWGPLIAGHAHPDVVRAVQEAAERGIGFGASTPIEVDLAERIVA
                     AFPSIEKVRLVTSGTEATMTAIRIARAATGRERIVKFAGCYHGHSDQLLARAGSGVAT
                     LGVPDSAGVPAGVTRFTSVVEYNDLAAVE"
     gene            966..2324
                     /gene="dnaA"
                     /locus_tag="VNL92_06955"
     CDS             966..2324
                     /gene="dnaA"
                     /locus_tag="VNL92_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWO94487.1"
                     /translation="MRSWQHAQETLRATLGNSAYKAWIKPLRFAAFENGKILLDAPNK
                     FYRDWLENNHLETVRKALTGDSGVAPEIVVRVAQGELFDEQDAEPPAERRERPKRRED
                     AAEVAARLAKLVPGYTFENFIVGPSNQLAHAAAMSIARSPGSTYNPVFIYGSTGVGKT
                     HLVNAIGHAIFQRSPSAKVVYLSCEAFVNELIISLRHKSMDEFKKRFRKVDVLIVDDV
                     QFLAGRERTQEEFFHTFNTLHAARRQIVLTSDKTPKDIPDLEERLRNRFEWGLIADIQ
                     APDKETTLAIVQQKAAALGVAIPPDVAAFLAENISSNVRELEGAIKRLAMKASLGKVE
                     VTVEFAEAALEPLLHKERKQTTIEDVQTVICEHFGLTLTQLKSKRRTQNVVVPRQIAM
                     FLARELLGASFPVIGEQFGGRDHSTVIHACSVIKARQDDDPALRATIERLARSISARR
                     GH"
     gene            2566..>3096
                     /gene="dnaN"
                     /locus_tag="VNL92_06960"
     CDS             2566..>3096
                     /gene="dnaN"
                     /locus_tag="VNL92_06960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWO94488.1"
                     /translation="MEFSIDRSEFMQGLSPTQGVVERRNTLPILANVLLEAGNDLAIS
                     ATDLEVHIRRRCTAKVKTPGAATVGARKLLELVRELKPGEITVRSLENQFVEVISGRS
                     RVKLVGLPASDFPAFPHGEEKAKPTFTIAPDVLAQMIDRTLFAVSTDETRPHLGGVYL
                     TSGEDTLRFVGTDGHRL"
BASE COUNT          566 a         1025 c          992 g          513 t
ORIGIN      
        1 ctccacggca gcgaggtcgt tgtactcgac gacgctcgtg aagcgcgtga ctcccgccgg
       61 cacgccggcg ctgtccggaa cgccgagcgt cgccacgccg gagccggcac gtgcgaggag
      121 ctggtcgctg tgtccgtggt agcaacccgc gaacttcacg atccgctcgc gccccgtcgc
      181 cgcgcgggcg atccggatcg cggtcatcgt cgcctcggtt cccgaggtga cgaggcgcac
      241 cttctcgatc gacggaaacg cggcgacgat ccgctccgcg agatcgacct cgatcggagt
      301 cgacgcgccg aagccaatgc cacgctccgc agcttcctgc accgctcgta cgacgtcggg
      361 atgcgcgtga cccgcgatga gcggtcccca cgcacacacg aggtcgacgt agcgacggcc
      421 ttcgcagtcc cagagatacg cgcccgagcc gcgattcacg aagcggggat tgcctccgac
      481 ggctttccac gcgcgaactg ggctgtttac gccgccggga atgacgtgcg ttgcgcgggc
      541 ggccagtttc aatgtcgagt tctgcaaagt gcgatcggca aaggtgaatc gacctcgttg
      601 cggctaccaa cgctccgcaa caccgtcaag gagcattcgc cgcgaagatt tttccgaagc
      661 aaaatcggac ctctcgaccg ttggcccgct tttcttcttg caacacccgg caacgcagcg
      721 ttagaaccgg cgccctcgta ccggcggggg tacgagctgc tcccggtttt ccacgactca
      781 acaagtggct tttcgacgcg cttcctcgct cgctgcacga cggccgggca aggtgaacca
      841 agcgccccac ggcgtgaaag aacgctggag aggacggcgt aaccgtcgga aaacacggga
      901 gaaaaccgtt ctccacagcc tgtggaaagt tctgtgcata agcctggcct cggggggcga
      961 gcaggatgag aagctggcag cacgcgcagg aaacacttcg tgcgacgttg gggaacagcg
     1021 cctacaaagc atggatcaaa ccgctccggt tcgccgcgtt cgagaacggc aagatcctgc
     1081 tcgacgcgcc gaataaattt tatcgtgact ggctcgagaa caaccatctc gagaccgtgc
     1141 gcaaggctct gacgggggat tccggcgtcg ctccggagat cgtcgtccgc gtcgcccagg
     1201 gcgagctgtt cgacgagcag gacgccgaac cgccggcgga gagacgagag cggcccaagc
     1261 gacgcgagga tgcggccgag gtggcggccc ggctcgcgaa gctcgtgccg ggctacacgt
     1321 tcgagaactt catcgtcgga ccgagcaacc agctcgcgca cgctgcagcg atgtccatcg
     1381 cgaggagccc gggatcgacc tacaacccgg tcttcatcta cggctcgacc ggcgtgggca
     1441 agacccacct ggtcaacgcg atcggccacg cgatcttcca gcggagccct tcggcgaagg
     1501 tcgtctatct ctcctgcgag gcgttcgtca acgagctcat catctcgctg cgccacaaga
     1561 gcatggacga gttcaagaag cgcttccgca aggtggacgt gctgatcgtg gacgacgtcc
     1621 agttcctcgc gggtcgcgag cgtacgcagg aggagttctt ccacacgttc aacactttgc
     1681 acgcggcgcg ccggcagatc gtgctcacct cggacaagac gcccaaggac atcccggatc
     1741 tcgaggagcg gctgcgcaac cgcttcgagt ggggcctgat cgccgacatc caagcgcccg
     1801 acaaggagac gacgctcgcc atcgtgcagc agaaggcggc ggcgctggga gtggcaatcc
     1861 cacccgacgt tgcggcgttt ctcgccgaga acatctcgag caacgtccgc gagctggagg
     1921 gcgccatcaa gcgcctcgcc atgaaggcgt cgctcggcaa ggtggaggtt accgtcgagt
     1981 ttgcggaggc cgctctcgag ccactgctcc acaaggagcg caagcagacg acgatcgagg
     2041 acgtccagac cgtgatctgc gagcacttcg gcctgacgct cacgcagctc aagtcgaagc
     2101 gccgcacgca gaacgtcgtc gtgccgaggc agatcgcgat gttccttgcg cgtgagctgc
     2161 tgggcgcttc cttcccggtg atcggcgagc agttcggtgg acgcgatcac tcgacggtca
     2221 tccacgcgtg cagcgtcatc aaggcccggc aggacgacga tccggcgctc cgtgcgacca
     2281 tcgaacgcct tgcgcgctcg atctcggccc ggcgcggcca ctgacgggga aagctgtgcg
     2341 ccggttgtgg acagcttgcc gatggtccgg ggacatcttc gcccgcatcg ctcgtcgtcg
     2401 tgcgagcgtg tcgttgtacg ctcgttcatc gacaggcgct ttccccgacc gtacggagac
     2461 ttgaccgggt tatccccgtt ctccacaagt cctactgctt ctaggtattt gaagcttcag
     2521 gtcgtctaga ggaatggaga ggggcgcgac gccgggagtg gtgtgatgga gttctcgatc
     2581 gaccgtagcg agttcatgca ggggctttct ccgacccaag gggtggtcga gcgtcgcaac
     2641 acgcttccga tcctcgcgaa cgtcctgctc gaggcaggca acgatcttgc catctcggcg
     2701 accgatctcg aggtccacat ccgccgccgc tgcaccgcga aggtgaagac gccgggcgcg
     2761 gcaacggtcg gcgcgcgcaa gctcctcgag ctcgtccgcg agctcaagcc cggcgagatc
     2821 accgtgcgct cgctcgagaa ccagttcgtc gaggtgatct ccggccgctc gcgcgtcaag
     2881 ctggtcgggc tcccggcgag cgacttcccc gcctttccgc acggcgagga gaaggccaag
     2941 ccgaccttca ccatcgcgcc ggacgtgctc gcccagatga tcgatcgcac gctgtttgcc
     3001 gtgtcgactg acgagacgcg tcctcacctg ggcggcgtgt acctcacctc gggcgaggac
     3061 accctgcgct tcgtcggcac cgacggacac cggctc
//
LOCUS       DATMBB010000323         6579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_303066, whole genome shotgun sequence.
ACCESSION   DATMBB010000323 DATMBB010000000
VERSION     DATMBB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303066"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VNL92_06965"
     CDS             complement(<1..1107)
                     /locus_tag="VNL92_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWO94489.1"
                     /translation="MYDLAPGDMMRFRHVEDVFRQVVTAWGYDEVRTPTLEYLHLFTE
                     AGTLSPQMLRRVYSFLDWDGWSGERVVLRPDATIPVARLYAERFPPGGDVRLAYVENV
                     FRFAEGDESREDWQCGVERLGGRAPEADLELILLGLELLERLQLPEPLVRVSHTGVAR
                     VVLAAAGLTPEEQLAHYDRILDGDLSALTEVEGSLPGLPLPLRTLFEVSGDLAFVTNL
                     GASFGPAIPGMRPPLEELAHIARVLSALGRRYDIRMALARDFEYYTGAVFRFEAGGER
                     MGGGGRYDALLRVVGGVDTPACGLALDVEPIVRLLPAMAIDRKLCVVRATSSDTGLMA
                     LAHEVAAGLREAGVVAAVNGPSGDGAMRVDVLASD"
     gene            complement(1266..1556)
                     /locus_tag="VNL92_06970"
     CDS             complement(1266..1556)
                     /locus_tag="VNL92_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94490.1"
                     /translation="MTPLVLVLLWSVPFAVLSLRCAQAGALPGARWFFIGLIIGPLGF
                     LACRRTVANATVGRKGSRSHISRLSLPESRISLHGRVLPKSEFDELLRSLGF"
     gene            1770..3518
                     /locus_tag="VNL92_06975"
     CDS             1770..3518
                     /locus_tag="VNL92_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94491.1"
                     /translation="MVRERGYWQRVSRRRISRRAAFRATAATGAGLSAMALVGCGDDD
                     DDDAEEQGPATGEARDEGEPVPGGTSRAGQTMAFNDVLDPHTSLALGAVMWSYIGNTA
                     LRLNREATQLEPELVASWEIPGDGTEIILTAQQGVQWHDKPPVSGRAFDAEDVAFNLM
                     RIAGLLNPDEAARFQRRTDLIGMNRAEAVDAQTTRVVMDRPASTFLNGLTDFRNSFIP
                     RDFLEKGGKLEDTPSLIGSGAFVLDTWQDGQRAVLAKNPNYFKAGMPYLDAVEWTWIP
                     DRLSLLTAFSNGDVDFFVSPTKVERETASRTANDMQEENWVYSNWNHLRFNTAREPFG
                     DPRVRRALFLALDYKAMADAYYGDGYWDFTGPGSAAFPEGIPSDEISQLPGWRSDGKD
                     EDIATAKQLMEDAGFPEGNISFKILVGGPTQDSVFYDYSIRAIDQWKQVWPAMDAQVD
                     LPPDFATFGLRQVRRDFDVIAYVIYPQPDILLDLISQYSETGSRNYGSFVDPRVDQLL
                     TTAFSQLDFEERSATLREVQDYLINEQMATITVNQPRLVTLFTSRVRGMTEFGGQADG
                     GLFDVLRHTEHMWLAD"
     gene            3565..4515
                     /locus_tag="VNL92_06980"
     CDS             3565..4515
                     /locus_tag="VNL92_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94492.1"
                     /translation="MTRYMMARLVAVIPTLLILLLFVVILVRLLPGDAVDIMLQDSAR
                     AGDVDRTELESRLGLDRSVPEEFFSYSADAFRGDLGRSVWSRQPVTDMISDRIGVTLE
                     LTVLSLVVATVIGIFVGVVSAVRQNSWLDYLLRGISIAGLSVPNFALATMVVVLPTIW
                     WGWSPSLIYTPPSEGLWDHISQFFLPAIILGLALSATLMRITRTMMLEVLRQDYIRTA
                     RAKGLRGRIVITRHALRNAMIPVVSLLGIQVAFLLSGAVIIENVFALPGIGRTLIASI
                     AQRDYPVVQGITVVSGVLVIASNLVVDLSYAWIDPRAKLG"
     gene            4546..5463
                     /locus_tag="VNL92_06985"
     CDS             4546..5463
                     /locus_tag="VNL92_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94493.1"
                     /translation="MTTDPHQTGLEAVGVLAPQPASRLAHSGAIFARFSRRQPIGMAS
                     AVILLVVALAAIFADLIAPYSPTRNDVGPALVGPNAEHLFGTDQFGRDVFSRVIHGAR
                     VSLYVGLGATLIGAAIATVLGATSGYLGGLFDYVLQRFVDAAQAVPPLVLLIGLLVVL
                     GPSVTNVIVALSVRQGLSLSRVVRGAVIGVRASPYIEAAQAIGASNVRTIALHVLPNI
                     LPTVIVLISTSIGGIIVAEASLSFLGYGVPPPTPSWGGMMSAEGRTYMIVASWILIFP
                     TVALSLVVFAMNMLGDALRDELDPRLRGG"
     gene            5611..5694
                     /locus_tag="VNL92_06990"
     tRNA            5611..5694
                     /locus_tag="VNL92_06990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5644..5646,aa:Leu,seq:taa)
     gene            complement(5737..>6579)
                     /locus_tag="VNL92_06995"
     CDS             complement(5737..>6579)
                     /locus_tag="VNL92_06995"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HWO94494.1"
                     /translation="IHNYIEGDELYFFGFSRGAYTVRSTAGMIRKVGLLDKVEADRAM
                     EAWRIYREHAGGPDSQRAIAFREAFSRYPVRIRFLGVWDTVGALGIPGALSFIGRRRF
                     QFHDVSLSRSVDYAYQALAIDERRRFFRPAVWKQHPEARGQVLEQAWFPGAHMDVGGG
                     HRDHRLADAALIWIADGARAAGLALNDAYMEQIADADPLGRIHESRKSFYRLIPAYDR
                     PIGTGGDDEPDEAVRSNERLHSAAEKRYELDSRYRPRGLVDYLARLGKRLAGGPGGGE
                     PGGD"
BASE COUNT         1136 a         2187 c         2115 g         1141 t
ORIGIN      
        1 atcagatgcc agaacgtcga ctcgcatcgc gccatcaccg ctaggtccgt tgaccgccgc
       61 cacaacaccc gcctctcgca ggcctgccgc cacctcatgg gcgagggcca tcagccccgt
      121 gtcggacgat gtggcgcgta ccacgcagag cttgcggtcg atggccatcg ccgggaggag
      181 gcgcacgatc ggctcgacgt cgagagcgag accgcacgcc ggggtatcca cgccgccaac
      241 cacgcgcagt agtgcatcgt aacggccacc gccacccatc cgctcgcccc cagcctcgaa
      301 gcgaaacacc gcgcctgtgt agtactcgaa gtcgcgggcc agggccatac ggatatcgta
      361 gcggcgcccg agcgcggaga gcacgcgggc gatgtgggcc agttcctcca ggggcggccg
      421 cataccgggt atcgccggcc cgaaggacgc tccgaggttg gtgacgaacg cgaggtcccc
      481 cgacacctcg aacagggtgc gcagcggcag cgggaggccc ggcaacgagc cctcgacctc
      541 ggtgagcgcg gaaaggtcgc catcgaggat gcggtcgtag tgcgccagtt gctcttcggg
      601 cgtgagtccg gcagccgcga ggacgacgcg ggcgaccccg gtgtgagaga cgcggacgag
      661 cggctccggc aactgcaggc gctcgaggag ctcgaggcca aggaggatga gctcgagatc
      721 ggcttcgggc gccctgccgc cgagccgctc gacgccgcac tgccaatcct cacgggactc
      781 atcgccttcc gcaaaccgga acacgttctc cacgtacgca agccggacgt cgccgccagg
      841 aggaaaccgt tccgcgtaga ggcgcgccac cggtatcgtc gcgtcggggc gcagaacgac
      901 gcgctcgccg ctccagccgt cccaatcgag gaacgagtag accctccgca gcatctgggg
      961 cgagagtgtg cccgcttcgg tgaacagatg caggtattcg agcgtcggag tccgcacctc
     1021 gtcgtagccc caggccgtca cgacctgacg gaagacgtct tcgacatggc gaaagcgcat
     1081 catgtcgccg ggcgccaagt catacatgcc gcggcagcgt tgggcggtgg ttcgttcgtt
     1141 tgtgaccagt tgcgggccgt ccttagctat cctcagtccg attctagccc gacgctgggg
     1201 ggtgaacaac cggccgcagg atgcgcgggc ctggagcgag cgatgcgggg cagcggggcg
     1261 atgcgtcaga agccgaggct tcgcaatagc tcgtcgaact ccgacttcgg aaggacgcgc
     1321 ccgtgcagcg agatgcgact ctccgggagc gacaggcgcg agatgtgcga ccgggaaccc
     1381 ttacggccaa ccgtggcgtt ggccacggta cgccggcagg cgaggaatcc gagtgggcca
     1441 atgatcagcc cgatgaaaaa ccaccgcgcg ccggggagtg ccccggcctg agcgcagcgc
     1501 aggcttagaa cggcgaaagg cacgctccag aggagaacaa ggacaagcgg cgtcatgcga
     1561 tggcctgctc tttccgtatc caccaggatt atcggccacc ggaccgaccg gcgtaggggt
     1621 ccgggagagg cgcacggacg gggactccga gcttccgtca cggccgctgt aaaccgacca
     1681 aacgtatggt atactccctc atctttgatt ggggctcggt gtccttacgc ggtagggcct
     1741 gatgttccta tcgatgaggg ggaatctcga tggtgcgtga gcgtggctac tggcaacgcg
     1801 tctcgcggcg gcgcatctcg cggcgcgctg cgtttcgcgc gacggccgca acgggggctg
     1861 gcctatccgc catggccctc gttggatgcg gagacgacga tgatgacgac gccgaggagc
     1921 aaggcccggc gacgggcgag gcgcgggacg aaggcgaacc ggttccgggc ggtacgagcc
     1981 gcgcgggcca aacgatggcc ttcaacgatg tgctcgaccc gcacacctcg ctcgcattgg
     2041 gcgccgtcat gtggtcgtac atcggcaaca ccgcgctgcg gctgaaccgc gaggccacac
     2101 aactcgagcc cgagctcgtc gcatcttggg aaattcccgg cgacggtact gagatcattc
     2161 tcacggcgca gcaaggcgtc caatggcacg acaagccgcc ggtgagcgga cgcgcctttg
     2221 atgccgagga tgttgccttc aacctcatgc ggatcgccgg gttgctgaac ccggatgagg
     2281 cggcccgctt ccagcgccga accgacctga ttggaatgaa tcgcgcagag gctgtcgatg
     2341 cgcagacgac ccgcgtcgtc atggaccgtc cagcgagcac cttcctgaac gggctcaccg
     2401 acttccggaa ctccttcatc ccacgcgatt tcctcgagaa ggggggcaag ctcgaagata
     2461 cgccaagcct catcggcagc ggcgctttcg tgctcgacac gtggcaggac gggcagcggg
     2521 ccgtgctcgc caagaacccg aactacttca aggccggcat gccctacctg gacgcagtcg
     2581 agtggacttg gattcccgac cgcctgagct tgctcaccgc cttctccaac ggcgacgttg
     2641 acttcttcgt ttcgccgacg aaggtcgagc gtgagaccgc gagtagaacg gccaacgata
     2701 tgcaggagga gaactgggta tattccaact ggaaccactt gcgcttcaac accgctcggg
     2761 agcccttcgg cgacccgcga gttcgccggg ccctcttcct cgcgctcgac tacaaggcca
     2821 tggccgatgc gtactacggt gatggctact gggactttac gggacccggc tcggccgcat
     2881 tcccggaggg gattccgagc gacgagatca gtcagcttcc cggctggcgt tcggacggga
     2941 aggacgagga tatcgccacg gcgaagcagc tcatggagga tgccggcttc ccggagggca
     3001 acatcagctt caagattctc gtcggtgggc cgacgcaaga ttccgtcttc tacgactaca
     3061 gtattcgcgc gatcgaccag tggaagcagg tgtggcctgc catggatgcg caagtcgacc
     3121 ttcccccgga cttcgcgacc ttcggtctcc gacaggtccg tcgcgacttc gatgtcatcg
     3181 cctatgtcat ctatcctcaa cccgatattc tcctcgatct catctcccag tattcggaga
     3241 cgggaagccg aaactatggc tccttcgtcg atccgagggt cgaccagctg ctaacgaccg
     3301 ccttctcgca gctggacttc gaggagcggt ccgcgacgtt gcgggaggtt caggattatc
     3361 tcatcaacga gcagatggcg acgatcaccg tcaatcagcc gcgactagtc acgctcttca
     3421 cctcgcgcgt tcgcggaatg acggagttcg gcggacaagc agacggcggc ctcttcgatg
     3481 tgctgcggca cacggaacac atgtggctag ctgactaggc gtgcgacggc cgacctcgcg
     3541 tcgcgacaga gagggccaat ttccatgact cgctatatga tggcgagact tgtcgccgtc
     3601 atcccgacac tgctcatcct cctcctgttc gtggttatcc tcgtgcggct cttgccgggc
     3661 gacgcggtcg acatcatgct tcaggactcc gcgcgcgccg gtgacgtgga ccgcacggag
     3721 ttggagagcc gccttggcct cgatcggtcc gtgcccgagg agttcttctc ctactcggcg
     3781 gatgccttcc gcggcgacct cggccggtcc gtctggtccc gacaacccgt aacggacatg
     3841 atctccgatc gtatcggcgt gacgctcgag ctgacggtcc tctcgctcgt agtcgccacg
     3901 gtcatcggaa tcttcgtcgg cgtggtttcg gcggtgcggc agaacagctg gctcgattac
     3961 ctcctccgag gcatctcgat cgccgggctc agcgtgccca acttcgccct cgcgacgatg
     4021 gtggtcgtgc tgcccacgat ctggtggggc tggtcgccgt cgctcatcta taccccgccc
     4081 agcgagggtc tatgggatca catctcgcag ttctttctcc ccgccatcat ccttggcctc
     4141 gccctcagcg ccaccctgat gcgcatcacc cggacgatga tgctggaagt gctacgtcag
     4201 gactacattc ggacggcacg tgccaagggt ctgcggggga ggatcgtgat cacgcgccac
     4261 gcactacgca acgcgatgat tcccgtcgtc tccctactcg ggattcaggt cgccttcctc
     4321 ctatccggcg ccgtgatcat cgagaacgtg ttcgcgctcc ccggtatcgg gcgcacactg
     4381 atcgcgtcca tcgctcagcg cgattatccg gtcgtacagg ggataaccgt tgtcagcggc
     4441 gtgcttgtca tcgcttcgaa tctcgtcgtc gacctctcct acgcatggat cgacccgaga
     4501 gcgaagttgg gctgagatgg gccgaggagc cggagggcca cgcctgtgac gaccgatccg
     4561 caccagacgg ggctcgaagc ggtcggcgtc ctcgccccgc agccggcatc gcgactagcg
     4621 cactcgggcg cgatcttcgc gcgcttttcc cggcgccaac ccatcggaat ggcatcggcg
     4681 gtcatcctcc tcgtcgtggc cctggcggcc atcttcgccg atttgatcgc gccgtactcg
     4741 ccgacgagaa acgatgtcgg gcctgcgctc gtagggccaa acgcggaaca cctcttcggg
     4801 acggaccagt tcggccggga cgtctttagc cgcgtgatcc acggcgcccg cgtgtcgctc
     4861 tatgtcggcc ttggcgcaac gctcataggc gcagcaatcg cgacggtgct gggagcaacg
     4921 agtggctacc tcgggggact cttcgattac gttctccagc gcttcgtcga cgcggcgcag
     4981 gcggtccccc cgctcgtgtt gctcatcggc ctgctcgtcg tgcttggccc gagcgtgacg
     5041 aatgtcatcg tggcgctttc ggtccgccaa ggcttatcgc tttctcgcgt ggtccgcggg
     5101 gccgtcatcg gtgtccgagc cagcccgtat atcgaggcgg cacaggctat cggcgcctcg
     5161 aacgtgcgaa ccatcgcgct gcacgtgctg ccgaacatcc tcccgacggt catcgtgctc
     5221 atctccacgt ccatcggagg catcatcgtc gccgaggctt cgctcagctt cctgggctac
     5281 ggcgtgccac caccgacacc atcctgggga ggaatgatga gcgcagaggg acggacgtac
     5341 atgatcgttg cctcgtggat cctgatcttc cctaccgtag cgctgagctt ggtcgtgttc
     5401 gcgatgaaca tgcttggcga tgctctgcgt gacgagctcg acccgcgcct ccgcggcggg
     5461 tagcgcccgc cttccggcgt gcatcggccg acgtgctcat gaggctccgt acccctcgcc
     5521 catccgcccg tgtcgtatag tctccctagt gaaccgagct ctcgctggcg cagggcgcga
     5581 gcgtgtggcc cgaggcccat gtcgtcgcgg gccggggtgg cgaaagggca gacgcggcgg
     5641 acttaaaatc cgctggggca acccgtgcgg gttcgaatcc cgcccccggc accagcagca
     5701 tcttcctccg atgcgctcgc aggctcgatg gcacgatcag tcgccgcccg gctcgccgcc
     5761 tcctggcccg ccggcgagcc gcttgcccag cctggccagg tagtcgacga ggccacgcgg
     5821 gcggtagcgc gaatccagct cgtaacgctt ctcagccgcg gaatgcagac gctcgttcga
     5881 gcgtaccgcc tcgtcgggct catcgtcccc acccgttccg ataggtcgat cgtaggcggg
     5941 aatgaggcga tagaaggact tgcgcgactc gtggatccga ccgagcggat cggcgtccgc
     6001 gatctgctcc atatacgcgt cgttgagggc gaggcccgcc gctcgggcgc catcggcgat
     6061 ccagatgagc gcggcgtcgg cgagccgatg atcgcgatgg cctcctccga cgtccatgtg
     6121 cgcgccgggg aaccatgctt gctccagcac ctgcccgcgc gcctccggat gctgcttcca
     6181 gacggcgggg cggaagaagc gcctccgctc gtcgatcgcg agcgcctgat aggcatagtc
     6241 aacgctccgg ctcaggctca cgtcgtggaa ctggaaccgg cggcgaccga tgaagctcag
     6301 cgcgccgggg atgcccaacg cccccaccgt atcccaaacg ccgagaaacc ggatacgcac
     6361 cggatagcgc gaaaaggcct cgcggaaggc tatcgcccgc tgggagtccg gacctccggc
     6421 gtgctcgcga tagatgcgcc aggcctccat cgcgcggtcg gcttcgacct tgtcgagcag
     6481 accgaccttg cgaatcatgc cggccgtgct gcgaacggtg taggcgccgc ggctgaaccc
     6541 aaagaagtag agctcgtcgc cctcgatata gttgtggat
//
LOCUS       DATMBB010000324         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_172849, whole genome shotgun sequence.
ACCESSION   DATMBB010000324 DATMBB010000000
VERSION     DATMBB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172849"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VNL92_07000"
     CDS             <1..472
                     /locus_tag="VNL92_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94495.1"
                     /translation="VAPPVAPSDGLAAETDRAWPRNSEVAAELARAGMEGRTVTLMVV
                     EAEARAWDDRLPSLRALAQVVEETTAGGARVVSDQGARLAVLLDGAEAAAAHELAQDL
                     CRQAAERGATGLRAGIAQFPRDGARPEDVIREAAEALAFAREAQLPVASSVLMG"
     gene            complement(515..1444)
                     /locus_tag="VNL92_07005"
     CDS             complement(515..1444)
                     /locus_tag="VNL92_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWO94496.1"
                     /translation="MDERERPLLDRLILPVLIPLGVLVVLAIVLYSLSRIFLGVSHDV
                     APAIAITISTFILLGAAYVATQPVSREQIAGMLAVPVVAIVIGGVFGELAYDGAEEEG
                     GEAGGPTTVAMSMGDNFFDPDTAEVTAGVPITFNLTNDGQAIHNMRIAGPDGEYDTDD
                     DAVSDPDTVRAGETATLEYSFPEPGEYAFRCDFHPTEMTGTVTAEEGEAGEGAQQVSM
                     VDNAFEPEEVLVPVGTEVTIEAVNNGQAVHNIRVAGPDGDFETDDDVVTVPEAVRGGE
                     TGVLTFTLPEGMDEMLFICDFHPTEMTGVIRPR"
     gene            complement(1444..2361)
                     /locus_tag="VNL92_07010"
     CDS             complement(1444..2361)
                     /locus_tag="VNL92_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWO94497.1"
                     /translation="MIPLVTSIGEAAAGNGALAASVEEASFSWTEWHPHPDVLVVIAA
                     LIGAYLFAWRAFGGPRNPENRLMRARLALYASGVGVIYLASGSPLHDLSETYLFSAHM
                     GQHMMLTLIGPPLLIAGTPGWMLRPLLRDPVVLQAARFLLNPLVALIAFNAVTVGTHL
                     PIVTDTALDYHAVHFLVHAVLVGTALQMWWQVFSPLPELPRLGEPWRMGYLFLQSIVP
                     TVPASFLTFASTPLYDFYAEAPRVWDVSVVADQRVAGLIMKLGGTIILWSLITVFFFK
                     WYEEEERDARGLPSWPEAEEELHRMGLMK"
     gene            complement(2451..2801)
                     /locus_tag="VNL92_07015"
     CDS             complement(2451..2801)
                     /locus_tag="VNL92_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWO94498.1"
                     /translation="MTMQHRWRSDRESAMSRAEALHEREEHPSQTTYIGIALILGVLT
                     LIEVWTYYIDGIQDVLPPILIVLAIAKFALVAGWFMHLRFDSTFYTVMFAGGLVLAAA
                     AFVIVLAVERALFV"
     gene            complement(2830..>2918)
                     /locus_tag="VNL92_07020"
     CDS             complement(2830..>2918)
                     /locus_tag="VNL92_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome oxidase subunit III"
                     /protein_id="HWO94499.1"
                     /translation="TVELAGLYWHFVDVVWIVIFTLVYLLPE"
BASE COUNT          532 a          943 c          969 g          474 t
ORIGIN      
        1 ggttgcgccc cccgttgctc cgtccgatgg ccttgccgcg gaaaccgacc gagcctggcc
       61 caggaattcc gaggttgctg cggagctggc ccgagccggc atggagggtc gaactgtgac
      121 gctcatggtg gtggaggcgg aagcgcgagc ctgggatgat cgcctgccgt cgctacgcgc
      181 cctcgcgcag gtcgtcgagg agacgaccgc cggcggcgct cgcgtcgtca gcgatcaggg
      241 cgcccgcctc gccgttctgc tcgatggcgc cgaggctgcg gcggctcacg aactggcgca
      301 ggacctgtgc cgccaagctg cggagcgcgg ggccacgggg ctgcgcgcgg gcattgccca
      361 gtttccgcgc gacggcgccc ggccggagga cgtcatccgt gaggcggcag aggcgctcgc
      421 gttcgcgcgc gaggcgcaac tgcccgttgc cagcagcgtc ttgatgggct agcggcggcg
      481 aaatgcagca ggcttcgcgc cggccttccg cacctcagcg aggtctgatg acgcccgtca
      541 tctccgtagg gtggaagtcg cagatgaaca gcatctcgtc catgccctcg ggcaaggtga
      601 acgtgaggac gccggtctcc ccgccccgga ccgcctcggg aaccgtgacg acatcgtcgt
      661 cggtttcgaa gtcgccatcg gggcccgcta ctcgtatatt gtgaaccgcc tgtccgttgt
      721 tgaccgcctc gatggtgacc tccgtgccaa ccggaacgag cacctcctcg ggctcgaacg
      781 cgttgtcgac catcgagacc tgctgagctc cttcgccggc ctcgccctcc tctgccgtca
      841 ccgtgccggt catttcggtc ggatggaagt cgcagcggaa ggcgtactcg ccgggctcgg
      901 ggaagctgta ctcgagggtt gccgtctccc ccgcccgcac tgtgtccggg tccgagacgg
      961 cgtcgtcatc cgtgtcgtac tccccgtccg gcccggcgat gcgcatgttg tggatcgcct
     1021 ggccgtcgtt cgtgagattg aacgtgatgg ggacgcctgc agtgacttcg gccgtgtcgg
     1081 ggtcgaagaa gttgtcgccc atgctcatcg ccacagtcgt cgggcccccg gcctcgccgc
     1141 cttcttcctc cgcgccatcg taggcgagct cgccgaacac cccgccgatg acgatggcca
     1201 ccacaggtac cgcaagcatg cccgcgatct gctctcgaga caccggttgc gttgccacgt
     1261 aggccgcgcc gagcaggatg aacgtcgaga tcgtgatggc gatggccggc gccacgtcgt
     1321 gcgagacccc cagaaagatc cgcgagagcg agtacaggac gatggcgagc acgacgagca
     1381 cgccgagggg gatcagcacg gggagaatca gccggtcgag gagtggtcgc tcgcgttcgt
     1441 ccattacttc atcaggccca ttcgatgcaa ttcctcctcg gcctcgggcc aactcggcag
     1501 gccgcgcgcg tctcgttcct cttcctcata ccacttgaag aagaacaccg tgatgaggga
     1561 ccacaggatg atcgtgccgc cgagtttcat gatgagcccg gcgacccgtt ggtcggcgac
     1621 gaccgacacg tcccaaacac gcggcgcctc cgcgtagaag tcgtacaacg gtgtcgacgc
     1681 gaacgtcagg aacgaggccg gcaccgtcgg gacgatcgac tgcaagaata gataccccat
     1741 tcgccagggc tcgccaagcc gcggcagctc gggcaggggg ctgaagacct gccaccacat
     1801 ctgcaacgcc gtcccaacga ggactgcatg gaccaagaag tgtaccgcgt ggtaatcgag
     1861 ggcggtgtcc gtcacgatcg gcaggtgcgt gccaacggtc accgcattga aggcgatgag
     1921 ggcgacgagc gggttcagca ggaagcgtgc cgcctgcaag acgacagggt cgcgcagcag
     1981 cggccgtagc atccatcccg gcgtgccagc gatgagcagc ggcggcccga tgagggtgag
     2041 catcatgtgc tgccccatgt gggcgctgaa gaggtatgtc tcgctgaggt cgtggagggg
     2101 cgatcctgac gctaggtaga taacgccaac accggaggca tagagcgcga gacgggctcg
     2161 catgagcctg ttttcggggt tgcggggacc gccgaacgct cgccaggcga agaggtatgc
     2221 cccgatgagc gcggcgatga ccacgagaac atcgggatgc ggatgccatt ccgtccaaga
     2281 gaagcttgct tcctcaactg atgccgcgag ggctccgttg cccgcggccg cctcgccgat
     2341 cgacgtaaca agcggaatca tggtctccac ccccctgggg aacctgcagg gggccggtcg
     2401 cgatcagcac ctgaccggcc gagcaacctt cagttacgag cagggcctcc tcagacgaag
     2461 agggcccgct cgaccgcgag cacaatgaca aacgcggcgg cggcgagcac cagtcctccg
     2521 gcaaacatga ccgtgtagaa cgtgctatcg aaccgaaggt gcatgaacca cccggccact
     2581 aatgcaaact tggcgatggc gagcacgatc aggatgggcg ggaggacatc ctggattcca
     2641 tcgatgtaat aggtccaaac ttcgatgagc gtgagcacac cgaggatgag ggcgatgccg
     2701 atataggtgg tttgcgatgg gtgctcctcg cgttcgtgga gcgcttcggc gcgcgacatc
     2761 gcgctctctc gatcggaacg ccaccggtgc tgcatagtca taccgctgtc acctcgaccc
     2821 tatcacccgc tactccggca gcaggtacac cagcgtgaag atcacgatcc agacgacatc
     2881 gacgaagtgc cagtagagcc ctgcgagctc gacggtca
//
LOCUS       DATMBB010000325         8495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_172966, whole genome shotgun sequence.
ACCESSION   DATMBB010000325 DATMBB010000000
VERSION     DATMBB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172966"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VNL92_07025"
     CDS             <1..978
                     /locus_tag="VNL92_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWO94500.1"
                     /translation="PLMMAAWKVAPALAAGCTIVLKPAEQTPLSALRLGELAMEVGFP
                     PGVLNVVPGFGETAGAALVEHPGVQKIAFTGSPEVGRTIMRSAAATLKRVSLELGGKS
                     PNIVFADADLDSAVRSASSGVFFNQGEVCSAGSRILVEDGVYDEFVGRLAERSRTITV
                     GDPLDPKTRMGAIVSGEQFERVTRYVEIGKKEGAQLVTGGGRVGERGYFVQPTVFANV
                     SNTMTIAQDEIFGPVASVIRFRDDEEAADVANATRYGLAAAVWTRDVKRAHRLARQVR
                     AGTVWVNSYGQTDTRSPWGGFGDSGFGRELGRQALDMYTETKVVWVALD"
     gene            1068..1559
                     /locus_tag="VNL92_07030"
     CDS             1068..1559
                     /locus_tag="VNL92_07030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWO94501.1"
                     /translation="MGLRLSRTRTPWVVVHAVILDGDRVLLVQRSNPRGWEIPGGFPE
                     PREYLTDAIVREVGEETGLSVRVERFSGLYYRTGFFSHISATFVCVVEDGDLVTSAET
                     TRAAYCPTNRLPPGLFPWYRAVIEDAVSGDGRPYIRRQHLGARAIARSVGIAVASQLG
                     VFK"
     gene            complement(1552..1938)
                     /gene="lepB"
                     /locus_tag="VNL92_07035"
     CDS             complement(1552..1938)
                     /gene="lepB"
                     /locus_tag="VNL92_07035"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWO94502.1"
                     /translation="MLMAARLVYKAVELATAMRCEIAGPSMEPALLRGDYVLISRLAY
                     LWREPKPGDVVVAKDPDVADRLIIKRIVHITADYRFVLRGDNEGQSRDSREFGAVARD
                     AILGKAWVRYWPPRRIGTVPRRIVVT"
     gene            complement(2113..2616)
                     /gene="sodN"
                     /locus_tag="VNL92_07040"
     CDS             complement(2113..2616)
                     /gene="sodN"
                     /locus_tag="VNL92_07040"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012323707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HWO94503.1"
                     /translation="MSFTSKLADWLRPNTTVHAHCDIPCAIYDPHQAQIGALSVIRMN
                     QLIEQLQMQGQAKEEHDRYIHQIARYTKVKEDHAEIVKHEVRVIWGDYFKPEHLEQYP
                     DLHSIVFNIMKLASKGRQEINMQAAQDLLSEVNRFAEIFWATKGVPTKRQPSNQTAGG
                     EFVVPAN"
     gene            2800..3558
                     /locus_tag="VNL92_07045"
     CDS             2800..3558
                     /locus_tag="VNL92_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWO94504.1"
                     /translation="MRAYQTISLEQRGRVARLTLTYAGGEPRIGPQLLGELADACESI
                     ADSRDIYSVVVASAAAVFGSGWSADALSAEATDLSHAFDCLAELPQPVVAALHGEASS
                     AALELGLACDVRIAGEGAKFSLPETAFGLIPLAGGAARLGRAVGRASAIDLVLTGRAI
                     DAAEALRMGLVSRVAPHSDLNDAALALAERIAERGPIATRYAKEAVNRGTGMTLEQAL
                     RFETDLTVLLQTTYDRAEGVRAFLEKRRPKFENR"
     gene            3570..3833
                     /locus_tag="VNL92_07050"
     CDS             3570..3833
                     /locus_tag="VNL92_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94505.1"
                     /translation="MQIILDAEEAWSLMTLLVSQVIDGPQLSSEGKDALRRWRSDRAV
                     GTVEMDELAEAMNEALGTVLDERTTKLLRRKGKYVSTKDPLRG"
     gene            3840..4079
                     /locus_tag="VNL92_07055"
     CDS             3840..4079
                     /locus_tag="VNL92_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94506.1"
                     /translation="MRVELDTDEAWALLSLVVNRLVDEAGLTDEDRARIRRWRSNEMK
                     PGSEAMRSLTAKLNEDLERAMRVKERSQIRKPDWR"
     gene            4091..4849
                     /locus_tag="VNL92_07060"
     CDS             4091..4849
                     /locus_tag="VNL92_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWO94507.1"
                     /translation="MSDVSHIVYEKDGPVAWVTLNRPDVLNALNLAMRDDLWSVLLAI
                     RDDVDVRAVVFRGAGERAFSAGADISEFGTAPSYIDARRARHERDVWGLMLAIPKPMV
                     AAIHGYALGAGCELSLCCDIRLASEDARLGVPETNLGYICSAGGTQTLPRTVPPGLAA
                     EMILTADPIDAERAYEAGLVHRVVPRERLYDEAAAIAHSLARLDPSVAAAAKRAVYES
                     LDMPPAEAVHANGLLAAQLLGRSPLPAAFTGGHM"
     gene            4846..5376
                     /locus_tag="VNL92_07065"
     CDS             4846..5376
                     /locus_tag="VNL92_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94508.1"
                     /translation="MTDAPAGGGPEARGFNWAGFLLLGAIVAVLGAVGYWAYDQSQGG
                     SGPLAHVDDLGNGEVRYFSDEHLYLVRTLDGQLLALDDRDTHPDYRGRECAVAWRPDV
                     ESPDGRGAFVGRCSGSQWSLAGGLLAGPSPRGLDRYATQVSGDEVDVDRSRLLCGEAP
                     SREAPQPVTSECTTGP"
     gene            5461..7053
                     /locus_tag="VNL92_07070"
     CDS             5461..7053
                     /locus_tag="VNL92_07070"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWO94509.1"
                     /translation="MNTVEFLTLSAAVVPDRVALACDDRTVTFEELAQRVTRLANVFQ
                     GLGVREGDAVAMMAMNSPEYVQAYYATAKVGGVFVPLNYRAKQEELVYMINTSDAKVL
                     LVGERYLPLVASIRDKLTTVEHYICVDAHPDDMANLGTLVAEASDDEIFVDVDDNQAS
                     IVIYTSGTTAMPKGVVLTFLTLSVYVTNTMNPADPTDERHEVTLLSVPLYHVAGATTM
                     LSSIWGGRMLAILPQFEPKAWLETVQRFRVTHAFVVPTMLKRIMEDPDFGNYDLSSLQ
                     LITYGAAPMPYDVVRRAVEVFECGLMNAYGQTESTSTLTYLGPEDHQIRGLEGEELER
                     KVRRLRSVGRPMDDVAISIQNEMGEILGPGEEGEICVSGPRVMREYLKQAEATQEAVR
                     DGWLHTGDVGYMDEEGYLFITGRKKDMIIRGGENISPGEIEAVLEEHDAVEEAAVIGV
                     PDPDWGEVVKAVVVLTPGKSATIEELQQYCKSRLASYKTPQYISFVDELPRNHLGKVL
                     KTDLRKFEGSAASEDHRATARV"
     gene            7061..>8495
                     /locus_tag="VNL92_07075"
     CDS             7061..>8495
                     /locus_tag="VNL92_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWO94510.1"
                     /translation="MQDQTYPHIRRSGRFRLILLAVFSVAWWQWFIAISAVSAGAVVA
                     GDELGFTEPQREVFWNITGGWSVYLMLALLVGVLLYGPYRRLQLWMLGRPGTVTTNLN
                     LRKRILNLFTQGLTTRRLPRYTYGGIMHTCIFVSFIALTIITLILAVDDDTPLDFLHG
                     DFYLWYSAAGDFFGIVGLVGVGMAVFARHTSAPAEMKRVRWEQRHQDDIIIFGLGALL
                     LSGLLAEAMRVGATELHQHSDWARWSFVAYGISGPLDAAGVSRDGFEAAHLVFWWFHV
                     PAAFVWLAYIGYSKLGHILYAPANSFLKTTDAYGKLPAITDFEERETFGVGRIQDFTW
                     KQLFELDVCVRCGRCTDNCPAAIAGQPLSPMHIIQDLKEHMNRVGPSLLTAEAVSGNG
                     NGAHEHEFEPMVGGAVRDEALWACRTCGACVQECPVFIEHIPTIVDMRRWLVMDEARM
                     PETVQGTLMNLEQRGHPWRGTALTRTSW"
BASE COUNT         1416 a         2644 c         2925 g         1510 t
ORIGIN      
        1 ccgctgatga tggccgcgtg gaaggtcgcc ccggccctcg cggccggctg caccatcgtg
       61 ttgaagccgg ccgagcagac cccgctgagc gccctccgcc tcggagagct ggccatggag
      121 gtcggctttc cgcccggggt cctgaacgtc gtaccaggat tcggcgagac ggcgggcgcg
      181 gcgctggtcg agcacccggg cgtgcaaaag attgcattca ccggcagccc cgaggtggga
      241 cgcacgatca tgcgctccgc ggcggctacg ctgaagcgag tctcgctcga attagggggc
      301 aagtcgccga acatcgtctt cgctgacgcc gacctcgatt ccgccgtgcg gtcggcgagc
      361 agcggcgtgt tcttcaatca gggcgaagtc tgctcggcgg ggtctcgtat cctcgtggag
      421 gatggcgtct acgacgagtt cgtggggcgg ctggcggagc gctcccggac catcaccgtc
      481 ggcgatccgc tcgacccgaa gacgcggatg ggggcgatcg tctcaggcga gcagttcgag
      541 cgcgtcacgc ggtacgtcga gatcggcaag aaggaggggg cgcaactcgt caccggtggg
      601 ggccgggtgg gggagcgcgg ctatttcgtg cagcccaccg tgttcgcgaa cgtgagcaac
      661 acgatgacca tcgcccagga cgagatcttc ggtccggtcg cgagcgtcat tcgcttccgc
      721 gacgacgagg aggctgccga cgtcgcgaac gcgacgcggt atggcctcgc tgcggccgtg
      781 tggacgcgcg acgtgaagcg ggcgcaccgc ctcgcgcgac aggtacgcgc gggcacggtg
      841 tgggtgaact cgtacgggca gacggacacg cgttcgccct ggggcggctt cggcgatagc
      901 ggcttcgggc gagagctcgg ccgtcaggct ctcgacatgt acaccgaaac gaaggtcgtg
      961 tgggtggcgc tcgattgacg gcaagcggcg ccaggggggc caggtggcag ccgaggaccg
     1021 caggcctcgc atcgcaggtc tactcctggc ttcgtcacta tctcagcgtg ggcctccgcc
     1081 tttcccgcac gcggacgcca tgggtcgtcg tccacgcagt catcctggac ggcgaccgcg
     1141 tactgctggt acagcgcagc aacccgcgtg gatgggagat tcccggcggt ttcccggagc
     1201 cgcgcgagta tctgacggat gccatcgtgc gtgaggtggg ggaggaaacg gggctcagcg
     1261 ttcgggtgga gcgcttctcc gggctgtatt accgtacggg gttcttctcc cacatctcgg
     1321 cgacgttcgt ctgcgtcgtc gaggacggcg atctcgtgac gagcgcggag acgacccggg
     1381 cagcctactg cccgacgaat cggctgccgc ctgggttgtt cccctggtac cgcgcggtca
     1441 tcgaagatgc ggtgagcggc gatggccgac cctacattcg ccgccagcac ctgggtgcgc
     1501 gcgccatcgc tcgatcggtc ggtatcgccg tcgcctcgca gctcggcgtg ttcaagtgac
     1561 cacgatgcgg cgcggcaccg taccgatgcg acgtggcggc cagtagcgca cccatgcctt
     1621 gcccaggata gcgtccctcg cgacggctcc gaattctctg ctatcgcggc tctgcccctc
     1681 gttgtcgccc ctcagcacga agcggtagtc ggccgtgatg tggacgatgc gcttgatgat
     1741 gagcctgtcg gcgacgtcgg gatccttggc gacgacgacg tcgcccggct ttggttcgcg
     1801 ccagaggtag gcgagccggc tgatcaacac gtaatcgccg cgcaggagtg ccggctccat
     1861 gctgggtccg gcgatttcgc aacgcatcgc tgtggccagc tcgacggcct tgtacacgag
     1921 gcgcgctgcc atgagcatcg cgatgacgcc tagcagggag cgccaaactc gcgaccgccc
     1981 gggcaggtgc cgacgggatg cagggggtgc gaaacgagag gggcggattc gcgttgtcga
     2041 taccacgtgt cggacgatgc gacggcctcc tgtgtgagga ggccgtcacg ttgtgttcga
     2101 actcttcgga tttcagttcg cgggaacgac gaattcgccg cctgccgtct ggttcgaagg
     2161 ttgccgcttg gtcggaacgc ccttcgttgc ccagaagatc tccgcaaagc ggttgacctc
     2221 cgaaagcagg tcctgcgcag cttgcatgtt gatctcctgg cgccccttcg aagcgagctt
     2281 catgatgttg aacacgatgc tgtgcaggtc gggatactgt tcgaggtgct cgggcttgaa
     2341 gtaatcgccc caaatcactc ggacctcgtg cttgacgatc tccgcgtggt cttccttgac
     2401 cttcgtgtac cgggcgatct ggtggatgta tcggtcgtgc tcttccttgg cctggccctg
     2461 catctgaagc tgctcgatga gctggttcat gcggatgacg gaaagggcgc cgatttgcgc
     2521 ttgatggggg tcatagatgg cacatgggat gtcgcaatgg gcgtgaacgg tcgtgttcgg
     2581 acggagccaa tcggcgagct tcgacgtgaa cgacatgggg catactcctt cttgcggtgc
     2641 tgtcgcctcg gcgaggttcg ggaaggacgc gagcctgcgc tcggcgccag tatagtgcgc
     2701 ctccctcgac ctcgccaagc atccggcact agcgtatctg ttccttgaca tacgatttcg
     2761 gccgttgcta cgctgccgcc actccaacaa cgaggcggca tgcgagccta ccagacgatt
     2821 tctcttgagc aacgcgggcg ggtagcgcgc ctgacgctga cgtacgccgg cggggaacct
     2881 cggataggac cccagcttct cggcgagttg gccgatgcct gcgaatcgat cgccgacagc
     2941 cgagatattt acagtgtggt cgtagcgtcg gcggcggcag tgttcggctc cggctggtcg
     3001 gcggatgcct tgtccgccga ggccacggac ctctcgcatg cattcgattg tctcgcggag
     3061 ctgccgcaac cggtggtcgc tgcgcttcac ggggaagcga gcagcgccgc actggaactc
     3121 gggctggcct gcgacgtccg gatagccggc gagggcgcca agttctcgct tccggagacg
     3181 gcgttcggcc tcattccgct cgcagggggc gcggctcgat tgggccgcgc ggtcgggcgc
     3241 gcgagtgcga tcgatctcgt gctcacgggg cgcgccatcg atgctgcgga ggcgctcagg
     3301 atgggcctcg tgagccgggt cgcgccgcat tccgacctga acgacgccgc gctggccctc
     3361 gccgagcgca tcgctgagcg tggaccgatc gcgacgcgtt acgcgaagga ggcagtgaac
     3421 cgaggcaccg gcatgacgtt ggagcaagcc ctgcgcttcg agaccgacct cacggtgctg
     3481 ctccaaacca cgtatgaccg ggcggaaggc gtgcgggcct tcctcgagaa gcgcaggcca
     3541 aagttcgaga atcgctgacg ggggacgaga tgcagatcat tctcgacgct gaggaggcgt
     3601 ggtcgctcat gacccttctc gtgtcacaag tcatcgatgg accgcagctc tcatccgagg
     3661 gcaaggatgc gctccgcagg tggcggagcg atcgggctgt ggggaccgtg gagatggacg
     3721 agctggccga ggccatgaac gaggcccttg gcacggtcct tgacgagcgg acgacgaagc
     3781 tgcttcgccg caagggcaag tacgtctcga cgaaggatcc gttgcggggg taatgaagcg
     3841 tgcgcgttga gctggatacg gacgaggcat gggcgctgct gtcgctcgtc gtcaatcgtc
     3901 ttgtcgatga ggcggggctg accgatgagg accgcgcgcg catacgccgg tggcgcagca
     3961 acgagatgaa gccgggaagc gaggcgatgc gatcgctgac cgcgaagctg aacgaggacc
     4021 tcgaacgggc catgcgcgtc aaggagcgta gccagatccg aaagcccgac tggcgctgat
     4081 ggaagcaccg atgtcagacg tctcgcacat cgtgtacgag aaggacggcc ccgtcgcttg
     4141 ggtgacgctg aaccggccgg acgtgctcaa tgccctgaac ctggcgatgc gcgacgatct
     4201 atggagcgtg ctgctcgcca tccgtgacga tgtcgacgtg cgagcggttg tgttccgcgg
     4261 cgccggcgag cgcgcgttca gcgcgggagc ggatatctcc gagttcggca cggcgccgtc
     4321 ctacatcgac gcccgccggg cccggcacga gcgcgacgtg tggggcctca tgctcgccat
     4381 cccgaagccg atggtggcgg ccatccatgg ctacgcgctc ggagctggat gcgaactaag
     4441 cctgtgctgc gacatccgcc tcgcgagcga ggatgcgcgc ctcggcgtgc cggagacgaa
     4501 tcttgggtac atctgttccg ccggcggcac gcagacgctg cctcgaacgg tcccgccggg
     4561 gctcgccgcc gagatgatcc tgaccgcgga ccctatcgat gccgaacgag cgtacgaagc
     4621 gggtctggtg catcgcgtcg tgccgcgaga gcgcctctat gatgaggcag cagccatcgc
     4681 gcactcgctc gcgaggctcg acccgagtgt cgccgccgcg gcgaagcggg cggtctacga
     4741 aagtctcgat atgccgccgg ccgaagccgt gcacgcgaac ggccttctgg cggcgcagct
     4801 cttgggtcgc tcgccgcttc cggcggcgtt caccggtggg cacatgtgac agacgctccg
     4861 gcagggggcg ggcccgaggc gcgcggcttc aattgggccg gctttctgct cctcggcgcg
     4921 attgttgccg tccttggagc ggtaggatat tgggcttatg atcagtcgca gggagggagc
     4981 gggcccctcg cgcatgtcga tgatctgggg aatggagaag tccgctactt cagcgatgag
     5041 cacctgtacc tcgtgaggac gctcgatggg caactcctcg ccctcgatga tcgcgacacg
     5101 catcccgact accgtggaag ggaatgcgcc gtcgcttggc ggccggatgt cgagtcgccg
     5161 gatggccgcg gcgcgttcgt cgggcgctgc agcggctcgc agtggtcgct cgctggaggc
     5221 ctccttgcag gtccgagccc gcgtgggctc gatcggtatg cgacgcaggt gagcggcgac
     5281 gaggtcgatg tcgaccgatc gaggcttctc tgtggggagg caccgagccg cgaggcgccg
     5341 cagccggtga cgagtgaatg cacaacgggt ccgtaagggg cgcatcaact gcgccctgtg
     5401 gaagtgctta cagagtgtga taggattgcg ctgcgcgccc gcgcccatgg aggcctaagc
     5461 atgaacaccg tggagttcct gaccctaagc gccgccgtcg tacccgaccg cgttgccctc
     5521 gcctgtgacg acaggaccgt cacgttcgaa gagctggccc agcgtgttac gcggctcgcg
     5581 aacgtcttcc aggggctcgg cgttcgtgag ggcgatgcgg tcgccatgat ggcgatgaac
     5641 tctcccgagt acgtgcaggc atactacgcc acggccaagg tcggtggcgt cttcgtgccg
     5701 ctcaactatc gggcgaagca ggaggagttg gtctacatga tcaacacctc cgacgccaag
     5761 gtcctcctcg ttggcgagcg ctacctgccc ctcgtggcct ccatacgcga caagctgacg
     5821 accgtcgagc attacatctg cgtggatgcc catccggacg acatggcaaa cctcgggacc
     5881 ctcgtggcgg aggcgagcga cgacgagatc ttcgtcgacg tggacgacaa ccaggcgtcc
     5941 atcgtcatct acacgagcgg cacgaccgcg atgccgaagg gcgtagtgct gaccttcctg
     6001 acgctctctg tgtacgtgac gaatacgatg aatccggccg acccgacaga cgagcggcac
     6061 gaggtgacgt tgctctcggt gccgctctat cacgtggccg gcgccacgac catgctctcg
     6121 tccatttggg gcgggcggat gctcgctatc ctcccgcagt tcgagccgaa agcctggctt
     6181 gagacggtcc agaggttccg cgtcacgcac gctttcgtgg tgccgaccat gctcaagcgg
     6241 atcatggagg atccggattt cggcaattac gatctgtcgt cgctccagct catcacgtac
     6301 ggagcggcgc cgatgccgta cgacgtcgtc cggcgggcgg tggaagtatt cgaatgcggc
     6361 ctcatgaatg catatggcca gacggagagc acctccacgc tcacgtactt gggacccgag
     6421 gaccaccaga tcaggggctt ggaaggggag gagctcgagc gcaaggttcg tcgcctccgc
     6481 tccgttggac gccccatgga tgacgtggcc atctcgatcc agaacgagat gggtgagatc
     6541 ctcgggcccg gcgaagaagg cgagatctgc gtcagtgggc cgcgcgtgat gcgcgagtac
     6601 ctgaaacagg ccgaggccac gcaggaagcc gtccgcgacg gatggctcca caccggcgat
     6661 gtcggctaca tggacgagga agggtacctg ttcattaccg gccgcaagaa ggacatgatc
     6721 attcgcggcg gcgagaacat ctcccccggc gagatcgagg ctgtcctcga agagcacgac
     6781 gccgtcgaag aggcggccgt catcggcgtg cccgaccccg attggggcga ggtcgtgaag
     6841 gcggttgtgg tgctcacccc tggcaagagc gctaccatcg aggagctcca gcaatactgt
     6901 aagtcgcgac tcgcttccta caaaacgccg cagtacatct ctttcgtcga tgagctgccg
     6961 cgcaaccacc ttggcaaggt cctcaagacg gacctgcgca agttcgaggg ctccgcagca
     7021 agcgaggacc accgcgcgac ggcaagggtg taggggcgtc gtgcaggacc aaacctatcc
     7081 tcatattcga cgctcgggcc gcttccggtt gattcttctc gccgtgttca gcgtcgcctg
     7141 gtggcagtgg ttcatcgcca tcagcgccgt ctccgccgga gccgtcgtgg ctggcgacga
     7201 gctgggcttc accgagccgc agcgcgaggt gttctggaac atcaccggcg gctggtctgt
     7261 ctacctgatg ttggcgctgc tcgtcggcgt cctcctctac gggccgtatc gccgcctgca
     7321 gttgtggatg ctgggccggc cgggcacggt gaccacgaac ctcaacctgc ggaagcgcat
     7381 cctgaacctc tttacccagg ggctcacgac gcggcggctc ccgcgttaca cgtatggcgg
     7441 catcatgcat acgtgcatct tcgtcagctt catcgcgctg acgatcatca ccctgatcct
     7501 cgccgtcgac gacgacaccc cgctcgattt cctgcacggc gacttctacc tgtggtactc
     7561 ggccgccggc gacttcttcg gcatcgtcgg actcgtcggc gtggggatgg ccgtgttcgc
     7621 ccggcatact tcggcccccg ctgagatgaa gcgcgtgcga tgggagcagc gacaccagga
     7681 cgacatcatc atcttcgggc tcggggcgtt gctgctctcc ggcttgctgg ccgaagcgat
     7741 gcgcgtgggc gcaacggagc tgcaccagca cagcgactgg gcccgatggt ccttcgtcgc
     7801 ctacggcatc tccggtccgc tcgacgcagc cggcgtaagt cgcgatgggt tcgaggcggc
     7861 ccatctcgtc ttctggtggt tccacgttcc ggcggcgttc gtgtggctcg cgtacatcgg
     7921 gtacagcaag ctggggcaca tcctgtatgc gcccgcgaat tcgttcctga agacgacgga
     7981 tgcgtacggg aagctgccag cgataacgga cttcgaggag cgcgagacat tcggcgtcgg
     8041 ccgcatccag gatttcacgt ggaagcagct gttcgagctc gatgtgtgcg tgcgatgtgg
     8101 ccgctgtacc gacaattgtc ccgcggctat tgcaggccag ccgctctcgc ccatgcatat
     8161 catccaggac ctgaaggagc acatgaaccg cgtcggcccg tcgctcctca ctgccgaggc
     8221 ggtgagtggg aacggcaacg gagctcacga gcatgaattc gagccgatgg tcgggggagc
     8281 cgtgcgtgac gaggcgctgt gggcgtgccg cacctgcggg gcctgcgttc aggagtgccc
     8341 cgtcttcatc gagcacatcc cgaccatcgt cgatatgcgc cgatggctgg tgatggacga
     8401 ggcccgcatg ccggaaacgg tgcaggggac cctgatgaac ctggagcagc gcggccaccc
     8461 ctggcgcgga acggcgctga cgcgaacgag ctgga
//
LOCUS       DATMBB010000326         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_451337, whole genome shotgun sequence.
ACCESSION   DATMBB010000326 DATMBB010000000
VERSION     DATMBB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451337"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3558)
                     /locus_tag="VNL92_07080"
     CDS             complement(<1..3558)
                     /locus_tag="VNL92_07080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWO94511.1"
                     /translation="MHIVGQFHPAVRTWFERRFVQPTEAQLAAWPHILCGSDTLLSAP
                     TGSGKTLAAFLAGIDGLVRRAEAGELPDEAAIVYVSPLKALSSDIQRNLETPLTEIRQ
                     VAAELGIELPETRVGLRTGDTTAQERQAMVRRPPHIVVTTPESLYLLVTSERGRAMLR
                     TVRTVIVDEIHALVRDRRGSHLALTLARLDHVALSRPVRIGLSATQRPIEEIARFLVG
                     AGATKDDGTPDCAIADLGHQRDIELAIEVPPSDLEAVAPGEQWADIYDRLAELVQEHS
                     TTLIFVNTRRTCERVAHHLTERLGGELVASHHGSISKDRRHNVERRLKEGSLRALVAT
                     ASLELGIDIGSVDLVCQVGSPRSIATFLQRVGRSGHALGLRPHGRLFPTTRDELLECA
                     ALVRAVRAGRLDAVRPHVAPLDVLAQQIVAECACEEWKEDDLFDLVRRAAPYRDLRRE
                     DFDEIVAMQANGVGGGAGRSPALLHRDRIGGVLRGRRGARMVAIQNGGTIPELGDYVV
                     VAEPDEVVVGTVNEDFAMESMQGDVFLLGSTSWRIRRVEQSTVRVVDAHGAPPTIPFW
                     LGEAPGRTTELSEEVGQLRRDVAAGLDDVEALLSRLNTECGLPEIGARQVVDYVRATR
                     DSLGLVPSDTDVVFERFFDESGGMQLVVHAPFGARINRAWGLTLRKRFCVTFDFELQA
                     AANDDAILLSLGPQHSFPLEDAFAYVTPGNAERSLVQAILYAPVFPTRWRWNATRALA
                     VPRQRSGKRIPPFLQRMRSDDLLAAVFPAQVGCQENVTGPLEVPDHPLVRQAVDDCLR
                     EAMDIDGLIAVLERIDAGQIRLHARDTTEPSPMAHEIVSGRPYTYLDDAPIEERRTRA
                     VSLRRALPENARDLGQLDAEAIELVRDEAWPAPRDAEETHDALLGLVAVRDGDVRDWR
                     PWLEALQVEGRAAMMQTAAERLWFAAESLHLVRLLYAEAQIPELRVPAHALVTAEERH
                     EARERLLRGHTEALGPITIGGLASRVALPEADVERGLAQLEAGGFVLRGRFTPGPGPE
                     EFCDRRLLARIHRYTLDRLRREIEPVSSQDYMRFLLRWHHLTPETALSGRAGLRLAIA
                     RLRGFEAPAIAWEHDMLARRIPEYHPGWLDELCLGGEVAWARLTPRRVATGRSAAGAT
                     RVTPVTLAPRGEMPVLLAAVRQFGSEAAQPPAAGAEAEVL"
     gene            complement(3622..>3931)
                     /locus_tag="VNL92_07085"
     CDS             complement(3622..>3931)
                     /locus_tag="VNL92_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HWO94512.1"
                     /translation="EIRSPNRQVRASGERMAVNMPIQGSQADLIKIAMVRLDDALRRN
                     GLRSRMLLQVHDELIFECPSDELDSMRSLCLEIMPNALPLCVPVEIDFKVGRTWGDAE
                     "
BASE COUNT          640 a         1307 c         1381 g          603 t
ORIGIN      
        1 cagcacctcc gcttctgcgc ccgccgccgg gggctgcgca gcctcgctcc cgaactgacg
       61 gactgcagcg aggagcaccg gcatctcgcc acggggcgcg agcgtcaccg gcgtgactcg
      121 ggtagcccct gccgccgacc gtccggtggc gacgcgacgc ggcgttagcc gggcccatgc
      181 tacttcgccg cccaaacaca gctcgtcgag ccagccaggg tggtattcgg gaatgcgtcg
      241 cgccagcatg tcgtgctccc aggcaatggc gggcgcctcg aatccgcgga gacgcgcgat
      301 ggcgagtcgc aggcccgcgc gtccgctcag tgccgtctcg ggcgtgaggt ggtgccaccg
      361 gaggaggaat cgcatgtagt cctgcgacga caccggttcg atctcgcgtc ggagccgatc
      421 gagcgtgtac cgatggatgc gcgccagcag gcgacgatcg cagaactcct cggggccagg
      481 ccctggcgta aagcgaccgc gcaacacgaa tccacccgcc tcgagctgtg ccagaccgcg
      541 ctcgacatcg gcctccggca aggcgacccg gctcgccagt ccgccgatag tgatgggccc
      601 cagcgcttcg gtatggccgc gcagcagacg ttcccgtgcc tcgtgacgtt cctcggcggt
      661 gacgagggca tgggccggga cccgcagctc agggatctgc gcctccgcgt acagcagacg
      721 cacgaggtgg agactctccg cggcaaacca gagccgctcg gcggcggttt gcatcatcgc
      781 ggcgcggcct tcgacctgaa gcgcctccag ccacggccgc caatcgcgaa cgtccccatc
      841 gcgcacggcg acgagtccga gcaaggcgtc gtgggtctct tccgcatcgc gcggcgcggg
      901 ccaagcctcg tcccggacga gctcgatggc ttcggcatcg agctggccga ggtctcgtgc
      961 gttctcgggc aacgcgcggc gcaggctaac ggcgcgcgtg cgccgttcct cgatcggcgc
     1021 gtcgtcgagg taggtgtacg gccgcccgct aacgatctcg tgggccatgg gcgatggctc
     1081 ggtcgtgtca cgggcatgca ggcgaatttg ccccgcatcg atgcgctcca acaccgcgat
     1141 gaggccatcg atgtccatcg cctcgcgcag gcaatcgtcg acggcttggc gaaccagcgg
     1201 gtgatcgggg acttccagcg ggcccgttac gttctcctga cacccgacct gcgcagggaa
     1261 gaccgcggcg agcagatcgt cggagcgcat gcgctgcagg aagggcggga tgcgcttccc
     1321 cgagcgttgg cgcgggacgg ccaaggcgcg cgtcgcgttc cacctccaac gagtcgggaa
     1381 gaccggtgcg tagaggatag cctggacgag cgaacgttcg gcattgccag gcgtcacgta
     1441 cgcgaaggca tcttccagag ggaaggagtg ttgggggccg agcgatagga gaatcgcgtc
     1501 atcgttggct gcggcctgca gctcgaaatc gaacgtcacg cagaagcgct tgcggagcgt
     1561 cagtccccaa gcgcggttga tgcgggcgcc gaagggcgcg tggacgacga gctgcatacc
     1621 gcccgactcg tcgaagaagc gctcgaagac gacgtcggta tccgacggaa cgaggccgag
     1681 cgaatcgcgc gtcgctcgca cgtagtccac gacctgccga gcgccgatct ccgggagacc
     1741 gcactccgtg ttgaggcggg agagcagcgc ttccacgtcg tcgagccccg cggcgacatc
     1801 gcgccgaagc tggccgacct cctccgacag ctcagtcgtc cggccaggcg cttcgccaag
     1861 ccagaatggg atggtgggcg gcgcgccatg ggcgtcgacc acgcggaccg tgctctgctc
     1921 gacacgacgg atgcgccatg acgtgctccc cagaaggaac acgtcgccct gcatgctctc
     1981 catcgcaaag tcttcgttga ccgtacccac gacgacttcg tccggctcgg ccaccacgac
     2041 gtaatcgccg agctccggga tcgtgccgcc attttggatg gcgaccatgc gagcgccgcg
     2101 acgtcctcga agcacgccac cgatgcggtc gcgatggagg agggcgggag accttccggc
     2161 gccaccgcca acgccgttcg cctgcatcgc gacgatctcg tcgaagtcct cgcggcgcaa
     2221 gtctcggtag ggcgctgcgc gtcgcaccag gtcgaacagg tcgtcctcct tccactcctc
     2281 gcacgcgcat tcggccacga tctgttgagc gagcacgtcg agcggtgcga cgtgcgggcg
     2341 gacagcgtcg agccggccgg cacgaacggc ccggacaagg gcggcgcact ccagcagctc
     2401 gtctcgcgtg gtcgggaaga ggcgcccgtg tggtcgaaga ccgagggcgt gcccggaccg
     2461 gcccacacgc tggaggaatg tggcgatgct gcgcggcgag cccacctgac acacgagatc
     2521 gacacttccg atgtcgatgc cgagctcgag cgaggccgtc gcgacgaggg cgcgcagcga
     2581 gccttccttc agccggcgct cgacgttatg gcgccggtcc ttcgagatgc tcccgtggtg
     2641 gctggcgacc agctcgccgc ccagccgctc cgtgaggtga tgcgcgacgc gttcgcaggt
     2701 acgccgcgtg ttcacgaaga tgagggtcgt cgagtgttcc tggacgagct cggccaggcg
     2761 gtcgtagatg tcggcccatt gctcgccggg cgcgacggcc tcgagatccg agggcggcac
     2821 ctcgatcgcg agctcgatgt cacgctggtg gccgaggtcg gcgatcgcgc aatcgggagt
     2881 gccgtcgtcc ttcgtggctc ccgctccgac gaggaagcgg gcgatctctt cgatgggccg
     2941 ttgcgtcgcc gagagcccga tgcgaactgg acgtgacaac gcgacgtggt cgagccgcgc
     3001 caacgtgagg gcgagatgac tgccacgccg gtcccgaacg agtgcgtgga tctcgtcgac
     3061 gatgaccgtg cggaccgttc gtagcatcgc ccgcccgcgc tcggacgtaa cgaggagata
     3121 gagggactcg ggcgtcgtga cgacgatgtg gggcggccgg cgaaccatcg cctggcgctc
     3181 ctgtgcggtg gtatcgccgg tgcggaggcc tacacgagtc tcaggaagtt cgattccgag
     3241 ctccgcggcc acctgccgga tctcggtgag cggcgtttcg aggttgcgct ggatgtcgct
     3301 actgagcgcc ttgagtggcg agacgtagac gatcgcggcc tcgtcgggaa gctcgcctgc
     3361 ctccgcccga cggacgagac cgtcgatgcc tgccagaaac gccgcgaggg tcttgccgga
     3421 gcccgtgggc gcgctgagga gggtgtccga cccgcacagg atgtgcggcc acgcggcgag
     3481 ttgcgcctcg gtcggctgca cgaaccggcg ctcgaaccag gtgcgtaccg ccggatgaaa
     3541 ctgccccacg atgtgcatgt ggccctccag gccgtcggag tatagcacgc ttgttcgatc
     3601 tgggccagct gcagagacga gctattcggc gtcgccccag gtgcggccga ccttgaagtc
     3661 gatctccacc ggaacgcaca agggcagcgc gttgggcatg atctcgaggc agagggagcg
     3721 catggagtcg agctcatcgc ttgggcactc gaagatgagc tcgtcgtgca cctggagcaa
     3781 catgcggctg cgcagcccgt tgcgccggag ggcatcgtcg agacggacca tcgcgatctt
     3841 gatgagatcg gcctgcgaac cttggatggg catgttgacg gccatccgct cgccgctcgc
     3901 ccgcacctgt cggttgggcg agcggatctc g
//
LOCUS       DATMBB010000327         6168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_14434, whole genome shotgun sequence.
ACCESSION   DATMBB010000327 DATMBB010000000
VERSION     DATMBB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14434"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VNL92_07090"
     CDS             complement(<1..1152)
                     /locus_tag="VNL92_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWO94513.1"
                     /translation="MTPGAPTHSEPPGSGLSFLRVNLVLASNVATALVLFAINVVLFR
                     ELGAAGKGHYTLFVLVTTLASGFTNLGVGLANVYFVARGKYDLHDLLAGAQLLLAVVA
                     LATAAAVGGAYLLDLDAEVFGELPGWLLIASIPATLQLAYVAPLLQAQNRFVAYNIAS
                     FIPSAGAILGVLVLVSLDELSTGRALAVWVCATVAADVFGMTAIGWAYYRRAFPFIPK
                     LSTLREQLRFGLQGEVGNLLQMVNFRLDQFVIVAFVDAAAVGIYSVAVAVSEGLWLLA
                     RATSVVLIPRLTATDDEEAADLAAFACRTLLLVAGLAAIAMAIVAPLVIEPIFGTDAE
                     GAILPTLLLLPGVLGTSGLYVTSSYLLSRGKPLLNSYSLAATAALTGVLD"
     gene            complement(1149..2336)
                     /locus_tag="VNL92_07095"
     CDS             complement(1149..2336)
                     /locus_tag="VNL92_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94514.1"
                     /translation="MRPLHNAGRPWPQPSGLLMTDRSPGFRRDLNVLLHYRWFIIVVF
                     AVAVVGGVLAGFANAFEYEATSVAEVRSLGRKPVFGEPFAINFEEYTFLGGTERVAER
                     AAGLLTELGRPTEPDDILGSISVRDVTPPGFQAFGLRQVEFKATDGDEDTALAYANAW
                     AQAYVAEAEERRSEVLEEQIDALDQQAQGALDEFNQALEDLTQALQAAPDALVGVPAE
                     ALDDRANVARALDRVRTIQSEQGEVSTDQLRVALGDVLPPQAIPDGATPSSLAAGLEL
                     QLAALNAEIAASEGDASQDNANDPQTAFARAQAARDTYDEALRQAAAGRASRAVPPVD
                     FRVVRGAEVENSQALGFLGLLAAAAAFGLAVGVVGAFVLDLLDRRGLLPWSRHRETAA
                     ERT"
     gene            complement(2788..4854)
                     /locus_tag="VNL92_07100"
     CDS             complement(2788..4854)
                     /locus_tag="VNL92_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWO94515.1"
                     /translation="MEFLIADTFTDSLARLTGEEQKAVKTTAFDLQLNPATPGFSVHR
                     IERARDPNFWSVRVTRGLRLIVHRTPASMLLCYVAHHDDAYQWAQRRKLETHPRTGAA
                     QLVEIRETFQEIVIPRYVEVERPKPKLFAGRSDVELLAYGVPPEWLADVKDADEDSIL
                     VLAGHLPAEAAEALLELAVGGTPVAPAPATVGADPFAHPDAQRRFRIMRDVEELERAL
                     EYPWEKWTVFLHPAQRELVERDYSGPARVSGSAGTGKTVVALHRAAHLARSNPDARVL
                     LTTFSEPLANALRVNLRRLISNEPSLAERLEVHSIGAIGLRLARPHLGDIRVATAQEV
                     AALIEKAATEVPGHRFTATFLQTEWDQVVDAWQLDSWEAYRDVQRLGRRTRLPEARRA
                     VLWSIYERVWRRLANQGMVTEARMFTRLAERLAAGSRPPFDHVVVDEAQDMSVPYMRF
                     LAALGGSGVNALFFAGDLGQRIFQQPFSWLALGVDVRGRSRTLRVNYRTSHQIRTQAD
                     RLLGPQVADVDGNIEERKGTVSVFNGPPPEIIVCPSEESERETVSRWIAQRVKEGVAP
                     YELGIFVRSKVQLDRARKAIEAAGLPHALLDEGAEATTGRASLGTMHFAKGLEFRAVV
                     VMACDDEVIPLQERIESIGDEADLEDVYATERHLLYVACTRARDHLLLTSVAPASEFL
                     DDLRME"
     gene            complement(4856..5605)
                     /locus_tag="VNL92_07105"
     CDS             complement(4856..5605)
                     /locus_tag="VNL92_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94516.1"
                     /translation="MSDEASGRWNPSDVAQYIAAAFTKLSHEGHWPDNVDGNAARLLI
                     QDLGEFAEVMVAAGRAHYWSPANALGRLFIERVEVLMGACVDNAVSKRYLDSVVAEPT
                     AERPNRPAKRVRPEDAVGRFLSRSGLGAEDQASFRAALVDVKNLYSEWFVHPRVMAPL
                     LSRAAGESENGVEEYWSHMIQLLAFGCSVSLLAANRLQVTIPVECVRALYAAGKCLEQ
                     LGNVYSEGVLDLHATIAERAVPEQAERIAGS"
     gene            complement(5602..>6168)
                     /locus_tag="VNL92_07110"
     CDS             complement(5602..>6168)
                     /locus_tag="VNL92_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWO94517.1"
                     /translation="PDLVDAAREAADAGFDVLIACAFNYGAHATEFNKLGRIPVLKAR
                     MNADLHMAGELKSTDKANLFVIFGEPDIEVAQAADGQIRVQVKGVDVFDPSSGEVRSD
                     GPDGIACWFIDTDYNGESFFVRHAYFLGANDPYSALKTTLKAEINEDAWATLHSDTSR
                     PFDKPESGRIAVKVINHLGDEVMKVFRV"
BASE COUNT         1078 a         1944 c         2105 g         1041 t
ORIGIN      
        1 gtcgagcacg ccggtgagcg cggccgtggc cgccaggctg tagctgttca ggagcggctt
       61 cccgcggctc aggaggtacg acgacgtgac gtacaggccg ctcgtgccca ggacgccggg
      121 aagcaggagc agcgtcggca gaatcgcgcc ctccgcgtct gtgccgaaga tcggttcgat
      181 gacgagcggc gccacgatgg ccatagcgat cgccgccagg cccgcaacga gcagcagcgt
      241 tcggcacgcg aaggcggcga ggtcggctgc ctcctcatcg tcggtcgccg tgaggcgcgg
      301 gatgagaacg acggatgtcg cgcgggcgag gagccagagg ccttccgaga cagcgacggc
      361 cactgagtag atgcccacgg cggcggcatc gacgaaggcg acgatgacga actggtcgag
      421 ccggaagttg accatctgca ggagattgcc aacctcgcct tgcaggccga agcgcagctg
      481 ctcgcgcagg gtcgagagct tgggtatgaa agggaacgct cggcggtagt acgcccagcc
      541 aatcgccgtc atgccgaata catcggcggc cacagtagcg cacacccaca cggcgagagc
      601 gcgtcccgtc gagagctcat cgagcgacac gaggacgagg acgccgagga tcgcacccgc
      661 cgagggtatg aaggacgcga tgttgtacgc gacaaagcgg ttctgcgcct ggagcagggg
      721 cgcgacatag gcgagctgca gcgtggccgg aatggacgcg atgaggagcc agcccggcag
      781 ctcgccgaaa acctcggcat cgaggtcgag gagatacgcg cctccgacgg cggcggccgt
      841 cgccaaggca acgacggcga ggaggagctg cgcacccgcg agcaggtcgt ggaggtcata
      901 tttgccccgg gcgacgaagt agacgttcgc cagtccgacg ccgaggttgg tgaagccgga
      961 cgcgagcgtc gtcacgagga cgaacagcgt gtagtgtccc ttgccggcag cacccagctc
     1021 gcgaaagagg acgacgttga tcgcgaagag gacgagggcc gtcgcgacat tcgaggcgag
     1081 gactagattg accctcagga aggataggcc gcttcccgga ggttcggaat gcgttggagc
     1141 gccgggcgtc acgtgcgctc ggccgccgtc tccctgtgcc ggctccatgg caagaggccc
     1201 ctgcgatcga ggaggtccaa gacgaacgcg ccgacgacgc cgactgcgag gccgaacgcg
     1261 gcggccgctg cgagcagccc gaggaagccg agcgcctgcg agttctcgac ttccgcccca
     1321 cgaacgacgc ggaagtcgac cggtgggacg gcacgagagg cacggcctgc agcagcctgg
     1381 cgcagcgctt cgtcataggt gtcgcgggca gcttgggcgc gggcgaaggc cgtctgtggg
     1441 tcgttcgcat tgtcctgaga cgcgtcgccc tcactcgctg cgatctcggc gttgagcgcc
     1501 gcgagttgca gctccaaccc ggccgcaagc gacgagggcg tggctccatc cggaatcgcc
     1561 tgcggtggca gcacgtcgcc gagggcgacg cgcaactgat cggtggagac ttcgccctgt
     1621 tcgctctgga tcgttcgcac gcggtcgagt gctcgcgcga cgttcgcgcg gtcgtcgagc
     1681 gcctcggccg gcacgccgac cagcgcatcg ggcgccgcct gcagcgcttg ggtgagatcc
     1741 tcgagcgcct ggttgaattc gtcgagcgct ccttgggcct gctggtcgag ggcatcgatc
     1801 tgctcctcga ggacttcgct gcgccgctcc tccgcctcgg caacatacgc ctgcgcccac
     1861 gcgttggcat acgcgagggc cgtatcctcg tcgccgtccg tcgccttgaa ctcgacctgc
     1921 cggaggccga aggcttggaa gcccgggggg gtgacgtcgc gcacggagat ggacccgagg
     1981 atgtcgtccg gctcagtggg gcgtccgagt tcggtcaaca gcccggcggc ccgttcggca
     2041 acgcgttccg tgccgccgag gaacgtgtac tcctcgaagt taatggcgaa gggctcgcca
     2101 aagacgggct ttcgccccag cgagcggacc tcggccacgg atgtcgcctc gtattcgaag
     2161 gcattggcga atccggcgag tacgccgcct acgacggcga cagcgaacac gacgatgatg
     2221 aaccagcggt agtgcagaag cacgttgagg tcgcggcgga agccaggcga tcggtcggtc
     2281 atcagcagtc cggacggttg cggccacggg cggccggcgt tgtggagtgg ccgcatgata
     2341 gcagagcgcc gatctacgcg ccatggagtc tgcgatgggg gaggatattg gtcggggtgc
     2401 ccagatttga attccatagg aggggttcgc aggcgtccgc aagcgtccgc gccagatacg
     2461 aatcggggga cattatgtcg cagaagtgcg tgggcgtccg ccgcgtaagg gcagtataag
     2521 ggcactgagg tccggcctct tacgagtccg tcagtcgaga gcgggagtcc cagctctgcc
     2581 acaacaatcg gaagcaaaag gacgcggaac ggcttatgga gcagcgcgtt gtttggttac
     2641 catataggtt cttccagcaa acgaccttaa ggtattcaga cgttcaagcg agcagtaaaa
     2701 gacttgtccc gcacatcgat cttttcgtgc gcggccggct cgagcgcagc cgcctacgcg
     2761 ggggctccct gctcttccgg ctgcacctta ttccatcctg agatcatcta ggaattcgga
     2821 tgccggagcg acgctcgtta gcaggaggtg gtcgcgggcg cgggtgcaag ccacgtagag
     2881 gaggtggcgt tcggtggcat agacgtcttc cagatcagcc tcgtcgccga tgctctcgat
     2941 ccgctcctgg agcgggatga cttcgtcgtc gcacgccatg accacgacag ctcggaactc
     3001 caggcccttg gcaaagtgca ttgtcccaag agaagcccga ccggtagtcg cctcggcgcc
     3061 ctcgtcgagc aatgcgtgag gcaggcctgc ggcctcgatc gccttgcgcg cacgatccag
     3121 ctgcaccttc gagcgaacga agatgcccaa ctcgtaaggt gcaactcctt ccttgacccg
     3181 ctgcgcgatc cagcgactca cggtttcgcg ttcgctttct tccgacgggc aaacgatgat
     3241 ctcgggtggc gggccgttga agaccgagac cgtgcccttt ctctcctcga tgttgccatc
     3301 aacgtccgcc acctgcgggc cgaggagccg gtccgcctgc gtgcggatct ggtgggacgt
     3361 gcggtaattg acccgaagcg tccgggagcg gccgcgcacg tcgaccccca gtgccagcca
     3421 cgaaaatggt tgctggaaga tgcgctggcc gaggtcgccc gcgaagaaga gcgcgttgac
     3481 gcctgaaccc ccgagagctg caagaaaccg catgtatggc acgctcatgt cctgcgcctc
     3541 gtcgacgacc acgtggtcga agggcggacg ggaacccgcg gccaggcgtt ccgcaagccg
     3601 cgtgaacatg cgtgcctccg tgaccatccc ctggttcgcg agccgccgcc agaccctttc
     3661 gtaaatcgac cagagcaccg cacggcgggc ttccgggagc cgcgttcttc gacctagacg
     3721 ctggacgtcg cgatatgctt cccaactgtc cagctgccag gcatcgacca cctggtccca
     3781 ttcggtttgg aggaaggttg cggtgaagcg atgcccgggg acctccgttg ccgctttctc
     3841 gatgagggca gctacttcct gtgccgttgc cacccgaatg tcgccgagat ggggtcgcgc
     3901 gaggcgcaga ccgatggcac cgatagagtg cacctccagg cgctctgcca gggagggctc
     3961 gttgctaatc aagcgtcgca ggttgacgcg cagggcgttg gccagcggct cggagaaggt
     4021 cgttaggagg acgcgggcgt ccggattgga ccgggcgaga tgcgccgcgc gatgcagggc
     4081 gacgaccgtc ttgcccgtgc ccgctgagcc ggacacgcgg gccgggccgc tgtagtctcg
     4141 ctccaccagc tcgcgctgag ccgggtggag aaagacggtc cacttctccc aggggtactc
     4201 gagcgcccgc tccagctctt ctacatcgcg catgatgcgg aagcgccgct gggcgtcggg
     4261 gtgggcgaat gggtcggctc cgacagtcgc cggagcggga gccacaggag tcccgccgac
     4321 ggcaagttcc aggagcgcct cggcggcctc cgcgggcaga tggccggcga gtacaaggat
     4381 gctgtcctcg tcggcgtcct tgacgtctgc gagccactcg ggagggacgc cgtaggcaag
     4441 tagttcgaca tcagagcggc cggcgaagag cttcggcttt gggcgctcca cctcgacgta
     4501 cctcgggatg acgatctcct ggaacgtctc ccggatctca acgagctgcg cggcgccggt
     4561 cctcggatgc gtctccagct tgcggcgctg cgcccattgg tatgcgtcat cgtggtgggc
     4621 gacgtagcag agaagcatgg aagctggcgt ccggtggacg atgaggcgga gacccctcgt
     4681 cacgcgtacc gaccagaagt tggggtcgcg ggcccgttca atgcggtgca cgctgaaccc
     4741 gggagtggcc ggattgagct ggaggtcgaa ggccgtcgtc ttgacggcct tttgttcctc
     4801 gccggtcagc cgcgcaaggc tgtccgtaaa ggtgtccgcg atgaggaact ccatatcaac
     4861 ttcccgcgat acgctctgct tgctcgggca cggcgcgctc ggcaatcgtt gcgtgtaggt
     4921 cgagtacacc ctctgagtac acgttgccaa gttgctcgag acacttgccc gctgcatata
     4981 gggctcgcac gcactcgact ggaatggtta cctggagtcg attcgcggcg agtaacgaga
     5041 cggagcaccc aaacgcgagt agctggatca tgtgcgacca gtactcctcc accccgttct
     5101 cgctctctcc tgcggcacga ctcagcagag gagccatcac cctcgggtgg acaaaccact
     5161 cgctgtatag gttcttcaca tcgaccaatg cggcccggaa gctggcttga tcttcggcac
     5221 cgaggcctga tcgtgagaga aaccggccga cggcatcttc gggccggacc cgcttcgccg
     5281 gtcgattcgg acgctcagcc gtcggctccg cgacgactga gtcgaggtat cgcttggaga
     5341 cggcattgtc cacacaagcc cccatcagta cctcgacgcg ttcgataaac agccgcccca
     5401 gggcgttcgc tgggctccag tagtgggctc ttccggccgc aaccatgact tccgcgaact
     5461 cgcccaagtc ctggatgagc agccgtgccg cgttgccgtc gacgttgtcg ggccaatgcc
     5521 cttcgtggct aagcttggtg aaagccgcgg cgatatactg agcgacgtcg ctcgggttcc
     5581 atcggccgga cgcttcgtcg ctcacaccct gaacaccttc atcacctcat cgccgaggtg
     5641 gttgatgacc ttgacggcga tgcggcccga ctccggcttg tcgaaggggc gcgaggtgtc
     5701 gctgtgcagg gtcgcccagg catcctcgtt gatctcggcc ttcagcgtcg tcttgagcgc
     5761 gctgtagggg tcgttggcgc ccaggaagta ggcgtggcgc acgaagaagc tctccccgtt
     5821 gtagtcggtg tcgatgaacc agcaggcgat cccatcgggt ccgtcgctcc ggacttcgcc
     5881 gctgcttggg tcgaagacat cgacgccctt gacctggacc cggatctggc cgtcggctgc
     5941 ctgcgcgacc tcgatgtcag gctcgccgaa gatgacgaag aggttcgcct tatcggtgct
     6001 cttgagctcg cccgccatgt gcaggtccgc gttcatccgc gccttgagga cagggatgcg
     6061 gcccagcttg ttgaactccg tcgcgtgcgc cccgtagttg aaggcgcagg cgatgagcac
     6121 gtcgaagccg gcgtcggccg cctcgcgggc ggcgtccaca aggtccgg
//
LOCUS       DATMBB010000328         4500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_410523, whole genome shotgun sequence.
ACCESSION   DATMBB010000328 DATMBB010000000
VERSION     DATMBB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410523"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VNL92_07115"
     CDS             complement(<1..417)
                     /locus_tag="VNL92_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase"
                     /protein_id="HWO94518.1"
                     /translation="MHIIPQLRKLERKYRDSLVVIGVHSSKFPNERDTGNIRSAILRY
                     GVEHPVVNDREMRVGMEYGFRSWPTLMFIDPEGRVFAVHEGEFPYDQMDALIGDMIRE
                     YDQAGLLDHRPLPYQLEAERMAVLPLLFPGKIEADVA"
     gene            complement(427..567)
                     /locus_tag="VNL92_07120"
     CDS             complement(427..567)
                     /locus_tag="VNL92_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94519.1"
                     /translation="MSPTKYAGKVNAPDFPAGLDWLNVDHPLSLAELRGKLVILDFWT
                     YC"
     gene            642..1100
                     /locus_tag="VNL92_07125"
     CDS             642..1100
                     /locus_tag="VNL92_07125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO94520.1"
                     /translation="MAVISVERVTTAAQMQEALEVRRRVFIEEQGVPEEEEIDPHDGD
                     PAIVRSAVHVLASLDGRVVGTGRLLLDYDRGENAHIGRVAVLRETRGVGVGRALMHAL
                     QDEARRRGFRGITLAAQLHAIPFYERLGYGPRGDVFLDAGIEHRWMDLEL"
     gene            1315..2295
                     /locus_tag="VNL92_07130"
     CDS             1315..2295
                     /locus_tag="VNL92_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94521.1"
                     /translation="MASQLNGHARRAQVAHVERDVILQTHRLTKRYGDLTAVDRLTIQ
                     LRRGEVYGLLGPNGSGKSTTVAMLLGLIKPTSGEIEAFGMSPRTHRWDILRQIGAIIE
                     SPAFYPYLSGRDNLKMMARSAADGSERRVDEVLERVDLTTRADDRYEHYSLGMKQRLG
                     IASTLLRDPRLIILDEPTNGLDPAGTLEIRALIPRLAEEGRTVMVCSHLLHEVQLVCD
                     RVTILQRGKQLVETSVDALLNQDRGLRLRTGNGTRPDELAPLLHQLPWVRSVAPEEDS
                     FVRVLAPADRGADITRFLADQGIYVAELTPIQPTLESIFLELTGGQSGDV"
     gene            2312..3166
                     /locus_tag="VNL92_07135"
     CDS             2312..3166
                     /locus_tag="VNL92_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWO94522.1"
                     /translation="MFKLRKRSLTWILLAILLSLILVLYLLLWTVTETVTDQVEQTQG
                     LFGTQTTVTEVTETLYLEQAIPFGLQITQLVGTFLGILLASASMGNEYGWGTIRSFLV
                     SSPARWQFLTGKIVAVSVMVFLGTLAAMVTAVVASTVISGVAGDLDYGFVDWQWLGDA
                     ALAYGRTVLAIAPYIAIAFFFAAYGRSTLAGIGAAVGILFLENLITSLMRLAGDLPSR
                     LTNVLPGANVETLMLQNELGLALNFQERSQTERIPLDPPFAALVLVAYIVALIAATYI
                     LFQKRDVE"
     gene            3261..4361
                     /locus_tag="VNL92_07140"
     CDS             3261..4361
                     /locus_tag="VNL92_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWO94523.1"
                     /translation="MHHRFNVAPRVVWSALLLALVLVGALLALSPLLGGKGSDGVEAQ
                     GQPPERKVIFVAGLFSSFSCQDLTDPNALSIESRDWATLWSSLKAQLPEFALGEDDVF
                     MFSYSGDYDCPGATPLSRANYAPIDTCASIDGSSGGQGQAERFDAWFQQLRQQYPNAT
                     WDIFGHSLGGLVVSYWASTADDASLAAVHSIVLMSSPLQGSSIVELITQLGNGEFSCF
                     DNTSSVLDISSRSNVISALNLPSGPVMCLLESDPAAVTARVPVLTVRNVRDIIVPVTT
                     SCLPGAADLVFFDNCEISGHLCVLSNPDVAASIADWMREGEAAVRADPGPSRRDRDTL
                     SGLVERVVRAIIDFIDDWADFDDGPQRTSDLL"
BASE COUNT          758 a         1442 c         1460 g          840 t
ORIGIN      
        1 cgcaacgtcg gcctcgatct tccccggaaa cagaagaggc aggacggcca ttcgctcggc
       61 ctcgagctgg taggggagag ggcgatgatc gaggaggccc gcttggtcgt actcgcggat
      121 catgtcaccg atgagcgcgt ccatctgatc gtatgggaac tcaccttcgt gaacggcgaa
      181 gactcggccc tccgggtcga tgaacatgag cgtgggccac gaccggaatc cgtattccat
      241 gccgacccgc atctcgcggt cgttcacgac agggtgctcg acaccatagc gcaggatggc
      301 gctacggatg ttcccggtgt cgcgctcgtt ggggaacttg gaggaatgta cgccgatgac
      361 gacgagcgag tcccggtatt tgcgttcgag cttccgcaac tgcggaatga tatgcatgca
      421 gttgattcag cagtaggtcc agaagtcgag gatgacgagc ttgcctcgca gctcggctaa
      481 ggacagcgga tgatccacat tcagccagtc gaggccggcc ggaaaatcgg gcgcgttcac
      541 cttgcctgcg tactttgtgg ggctcattgg ggctcctttc gcagcaaccg catcccggcc
      601 tgtgtctgcc tgggacaatg ccagcgtaca atcttcgcgc catggctgtc atatcggtcg
      661 agcgggtgac gacggcggct cagatgcagg aagcgctcga ggtgcgccgc cgcgtcttca
      721 tcgaagagca gggcgtgccc gaggaggaag agattgaccc gcacgatggc gatccggcga
      781 tcgtgcgcag tgccgtccac gtgctggcct cgctcgatgg ccgcgtagtg ggtaccggcc
      841 gccttctcct ggactacgac cggggcgaga atgctcacat cggtcgcgtc gcggttctcc
      901 gcgaaacgcg cggcgtcggt gtggggcgcg ccctcatgca cgcgctacag gacgaggcgc
      961 ggaggcgagg cttccgcggc atcaccctag cggctcagct gcatgcgatc ccgttctacg
     1021 agcgcctcgg atacgggccc aggggcgatg tctttctcga tgcaggcatc gagcaccggt
     1081 ggatggacct ggagctctga gggctacgcc cgcctttttc tcaacccttg ctcagccgcc
     1141 gtccgttacg cttggtacaa ggcgcgaata gcagctatac ttaatgtctc ggcgtcgtgt
     1201 cgcgctcact gtccgcgctc gtcgctcctt ccggcaagag actgcggagg gtatcggaat
     1261 gacgagtggc ggcctgattc ggccgaagcg acgcagtaaa ggggaaccat ccgggtggca
     1321 tcgcaactga acggccacgc gaggcgggcg caggtcgctc acgtcgagcg tgacgtcatc
     1381 ctccagacac atcgcctcac gaagcgttac ggcgacctca cggccgtcga tcgtctcacg
     1441 atccagctac gccgcggcga ggtgtatggc ctgctgggac cgaacggctc cggcaagagc
     1501 acgacggtcg ccatgctgct cggactcatc aagcccacgt caggcgaaat cgaggcattc
     1561 ggcatgtcgc cgcgcaccca tcgctgggat atcctccggc agattggcgc gatcatcgaa
     1621 tctccggcct tctaccccta cctttcaggt cgagacaatc tgaagatgat ggcacgaagc
     1681 gcggccgacg gctccgaacg gcgggtcgac gaggtgttgg agcgggtgga cctcacgaca
     1741 cgcgcggacg atcgctacga gcactactcg ctgggcatga agcagcgcct cggcatcgcc
     1801 tccaccctcc tacgcgaccc gcgcctcatc atcctcgacg agccgacgaa cggcctggac
     1861 cccgcgggga cgctcgaaat tcgcgccctc atcccgcgcc tcgctgagga gggacgcacg
     1921 gtgatggtgt gtagccacct gctgcacgaa gtccagctcg tgtgcgaccg cgtcacgatc
     1981 ctgcagcgcg gcaagcagct cgtcgagaca tcggtggacg cactgctcaa tcaggatcgc
     2041 ggcctgcgtc tgcgcacggg caacggaacg cgcccggacg agctggctcc gctcctgcac
     2101 cagcttccct gggtgcgatc ggtggccccg gaggaggact cgttcgtgcg ggtgctcgct
     2161 cccgcggacc gcggcgccga tatcacgcgg ttcctcgccg accagggcat ctacgtcgcg
     2221 gagctcaccc ccatccaacc cacgctcgag tccatcttcc tcgaactcac cggaggccag
     2281 agtggcgacg tttaatcaca ttggggtgga gatgttcaag ctacggaagc gtagcttgac
     2341 gtggatcctc ctcgccatcc tcctgtcgct catcctggtg ctctacctgc tgctgtggac
     2401 ggtcaccgag accgtgaccg accaggtcga gcagacacag ggactcttcg ggacccagac
     2461 caccgtgacc gaagtgaccg agacgctgta cctggagcag gccatcccgt tcggcctgca
     2521 gattacccag ctcgtcggga cgttcctcgg catcctgctc gcttccgcga gcatgggcaa
     2581 cgagtacgga tggggcacca tccgctcgtt cctcgtgtcg tcgccggcgc gatggcagtt
     2641 cctcactggg aagattgtcg ccgtgagcgt catggtcttc cttggcacac tagcggcgat
     2701 ggtcacggca gtcgtcgcct caaccgtcat ctctggggtg gccggcgacc tcgactacgg
     2761 attcgtggat tggcagtggc tgggcgacgc ggcgctggcg tacgggcgaa cggtgctggc
     2821 catcgcaccc tacatcgcca tcgccttctt cttcgcggcc tacggccgct cgacgctcgc
     2881 gggcatcggc gcggcggtgg gcatcctctt cctggagaac ctgatcacga gcctgatgcg
     2941 cttggctggc gacctgcctt cgcgtctcac gaatgtgctc cccggtgcca acgtggagac
     3001 gctgatgctg cagaacgagc tgggtttggc actcaatttc caggagcgga gccagacgga
     3061 gcgcatcccg ctcgatccgc cgttcgcggc gctcgttctc gttgcgtaca ttgttgcgct
     3121 gatcgcggcc acgtacatcc tgttccagaa gcgagacgtt gaataggcgt cgagcgatcg
     3181 tgagatttac gtgatgcggg gtgacgcgaa tgcttcgcgt gcagtagaat cagcggatgg
     3241 cccccaagga gggtactaag atgcatcacc ggttcaacgt cgcgccccga gtggtctggt
     3301 cggcgctcct gctggccctc gtgctcgtcg gggcgctcct cgcgctgtcg ccgcttctcg
     3361 gcggcaaggg ctccgatggg gtcgaggcac aagggcagcc gccggagcgt aaggtcatct
     3421 tcgtcgcggg gctgttcagc tccttttcct gccaagacct gaccgatccg aatgcgctgt
     3481 caatcgagag ccgggactgg gcgacgctgt ggtcctcgct caaggcgcaa ctgcccgagt
     3541 tcgcgctggg cgaagatgac gtcttcatgt tcagctattc gggcgactac gactgcccgg
     3601 gcgcgactcc gctgagccgc gcgaactacg ctcccatcga tacatgcgcg agcatcgatg
     3661 ggagtagtgg cggccaagga caggcggaac gattcgacgc ttggttccag cagctccgcc
     3721 agcagtaccc gaatgcaacg tgggacattt tcgggcacag cctcggcggg ctcgtcgtga
     3781 gctattgggc ctcgaccgcc gacgatgcct cgcttgccgc cgtgcattcg atcgtcctca
     3841 tgtcgagccc gctgcagggt agcagcatcg tcgagctgat tacgcagctc ggcaacggcg
     3901 agttcagctg cttcgacaat acgtcctcgg ttctggacat cagcagtcgc tcgaacgtga
     3961 tcagcgcgtt gaatctgccc tctggtcccg tgatgtgcct gctcgagtcg gacccggctg
     4021 cggtgacggc gcgagttccc gtgctgacgg tgcgcaacgt tcgcgacatt atcgtgccgg
     4081 tgacgacatc gtgcctacct ggggcggccg atctcgtgtt cttcgacaac tgcgagatat
     4141 cgggccatct gtgcgtcctc tcgaatcccg acgtggccgc gagtatcgcg gactggatgc
     4201 gagagggaga ggccgccgtg cgagcggacc caggcccttc gcgccgggac cgcgatacgc
     4261 tgagcgggct cgtggagcgg gtggtacggg cgatcatcga cttcatcgac gactgggccg
     4321 acttcgacga tggcccgcag cgaacctctg acctgctgta ggttcggtcc gacggctagg
     4381 cgccgtccgc gcgggcgctc gtattggccg cagcagcagc tgcggccttc tttcgccgcg
     4441 gcgccggttc ggcaacctct ggcgcggccg gcttcgactt cgcgaacagc gaagccccgg
//
LOCUS       DATMBB010000329         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_57954, whole genome shotgun sequence.
ACCESSION   DATMBB010000329 DATMBB010000000
VERSION     DATMBB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57954"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..2250
                     /gene="fusA"
                     /locus_tag="VNL92_07145"
     CDS             181..2250
                     /gene="fusA"
                     /locus_tag="VNL92_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWO94524.1"
                     /translation="MARPYPLERVRNIGIIAHIDAGKTTVTERILFYTGRTYKIGETH
                     EGTAVMDWMEQERERGITITSAATTAEWKDHAINIIDTPGHVDFTVEVERSLRVLDGG
                     IVVFDAVAGVEPQSETVWRQADRYNVPRICFVNKMDRMGADYWRTIDMILERLGAIPV
                     PIQLPIGAEADFEGMIDLVEMQAITFSDEKGEDQVIGPIPERLQEEADRRRDEMIERV
                     AESDEQLMINYLEGHEITTIELKKAMRRATLAGAITPVLCGAALRNKGVQPLLDAVVD
                     YLPAPDDIPPVKGLVPGTDQTVERHADDDEPFCAIAFKIVSDPYVGRLAYFRVYSGKV
                     AAGQQVMNTTRGDRERFGRILRMHANHREEVTEVYAGGIAAAIGLKRTFTGDTLSDPA
                     HPIILEAIRFPEPVLSVAIEPKTKDDQDKMGEALNRLAEEDPTFRLRFDKETGQTIIS
                     GMGELHLEVVVDRLQREFRVDANVGRPQVAYRETIRKPVKSEGRFVRQTGGRGQYGHV
                     WLEIEPQERGKGFEFEDAIVGGVVPREYIPAVQSGVREALDAGVLAGYPVVDIKVRLV
                     DGSYHPVDSSEMAFKTAASIGFKEGMRKADPVLLEPIMRIEVRTPDQFFGDILGDLNS
                     RRGQIQGVEQVGNLHVIRALVPLAETFGYATAVRSLSQGRATYSMEFDHYAEIPASVA
                     GAVGARR"
     gene            2269..>2408
                     /locus_tag="VNL92_07150"
     CDS             2269..>2408
                     /locus_tag="VNL92_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWO94525.1"
                     /translation="MAKQKFERTKPHVNIGTIGHVDHGKTTLTAAITKVLALKGEAEY
                     RS"
BASE COUNT          470 a          763 c          787 g          388 t
ORIGIN      
        1 gatccgccgc aaggatgaga cgcaccgtat ggcggaggcg aaccgcgcct tcgtccacta
       61 ccgctggtag ctagcggcaa gcggcggttt cccccgcctc gattcgacaa caaaagacgc
      121 cggcccggcg cttgccggcc gccgcgaagc aaggtcgccc gcaagggcaa gtgaggaagt
      181 atggctcggc cctacccgct cgagcgggta cgcaacatcg gcatcatcgc gcatatcgac
      241 gccggcaaga cgacggtaac tgagcgcatc cttttctaca ccggccgcac ctacaagata
      301 ggtgagacgc acgaaggtac ggccgtgatg gactggatgg agcaggagcg cgagcgcggc
      361 atcaccatca cgtccgccgc aacgacggca gagtggaagg atcacgcgat caacatcatc
      421 gatacacccg gccacgtcga cttcactgtc gaggtcgagc gtagccttcg cgtcctcgat
      481 ggcgggatcg tggtcttcga cgcggtcgcg ggtgtcgagc cgcagtcgga gacggtgtgg
      541 cgacaggccg atcgctacaa tgttccccgc atctgcttcg tcaacaagat ggaccggatg
      601 ggcgccgatt actggcgcac gatcgacatg atcctcgagc gcctcggcgc catccccgtt
      661 cccatccagt tgccgatcgg cgccgaggcc gacttcgagg gcatgatcga ccttgtggag
      721 atgcaagcca tcaccttctc cgacgagaag ggcgaggatc aggtcattgg tccgatccca
      781 gagcgtctgc aagaggaagc tgatcggcgc cgcgatgaga tgatcgagcg cgtcgcagag
      841 agcgacgagc aactcatgat caactatctc gaaggtcacg agatcaccac gatcgagctg
      901 aagaaggcga tgcgacgcgc gacgctagcc ggcgccatta cgcccgtcct gtgtggcgcc
      961 gccctgcgca acaagggcgt gcagccgctg ctcgacgctg tcgtcgacta tcttcctgct
     1021 ccggacgaca ttccgcccgt gaagggcctc gttcccggaa ccgaccagac cgtcgagcgt
     1081 cacgctgatg atgacgagcc cttctgcgcc atcgcattca agatcgtctc cgacccttac
     1141 gtcggccggc tggcgtactt ccgtgtctac tccggcaagg tggcggccgg gcagcaggtg
     1201 atgaacacca cgcgtgggga ccgggagcgc ttcggccgca tcctccgcat gcacgcgaac
     1261 caccgggagg aggtcaccga ggtctatgcc ggcggcatcg ccgccgcgat aggactgaag
     1321 cggaccttta ccggcgatac gctcagcgat ccggcgcacc ccatcattct cgaggccatc
     1381 cgcttcccgg agccggtgct ctcggtcgcc attgagccga agaccaagga tgatcaggac
     1441 aagatgggcg aggcactcaa ccgcctcgcc gaagaagacc cgacgttccg ccttcggttc
     1501 gacaaggaaa ccgggcaaac catcatctcg ggcatgggcg aactgcacct cgaggtggtg
     1561 gtcgaccgtc tccagcgcga attccgtgtc gatgccaacg tcggccgtcc gcaggtcgca
     1621 taccgcgaga cgatccgcaa gccggtcaaa tcggagggtc gattcgtgcg gcaaacgggc
     1681 ggacgcggac aatatggcca cgtctggctg gagatcgagc cccaggagcg aggcaagggc
     1741 ttcgagttcg aggatgccat cgtgggcggg gtcgtcccgc gggagtacat ccccgccgtg
     1801 cagagcggcg ttcgcgaggc tctcgatgcg ggcgttctcg ctggctaccc agtggtcgat
     1861 atcaaggtgc gcctcgtcga cggcagctac cacccggtcg actcgtcgga gatggcgttc
     1921 aagacggctg cttcgattgg cttcaaggag ggcatgcgca aggctgaccc tgtgctgctg
     1981 gagcccatca tgcgcatcga ggttcggacg cccgaccagt tctttggcga tatcctcggc
     2041 gacctgaact cgcgtcgggg gcagatccag ggtgtcgagc aggtgggcaa tctccacgtg
     2101 attcgcgcgc tcgtgcccct cgccgagacc ttcggctacg cgaccgcggt ccgatcgctc
     2161 tcgcaggggc gggcaacgta cagcatggag ttcgaccatt acgccgagat ccccgcttcg
     2221 gtcgcgggcg cagtgggcgc gcggcggtaa gggcaaggag gcacagccat ggccaagcag
     2281 aagttcgagc ggacgaagcc gcacgtaaac atcgggacga tcgggcacgt cgaccacggg
     2341 aagacgaccc tgaccgcggc catcacgaag gtgttggcat tgaaggggga ggcggagtac
     2401 cggtcgtt
//
LOCUS       DATMBB010000330         2807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_254808, whole genome shotgun sequence.
ACCESSION   DATMBB010000330 DATMBB010000000
VERSION     DATMBB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254808"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VNL92_07155"
     CDS             complement(<1..300)
                     /locus_tag="VNL92_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWO94526.1"
                     /translation="MDLVWLVPATGLLAILFAIWLARDVLARDPGTPEMQEIASMIFE
                     GAMAFLRRQYTTIAVLSVVVAIIIGVVVGAVSEGVKLIVFDGETIRYGDKVIEPWE"
     gene            complement(603..1628)
                     /locus_tag="VNL92_07160"
     CDS             complement(603..1628)
                     /locus_tag="VNL92_07160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO94527.1"
                     /translation="MRIGVLGAAGRIADLIALAKRVEELGYDSIWVPEFHNSDGFVRM
                     TAVALNTSRLTVASGIVNAFNRAPAMVAAAAMDIDELSDGRLILGIGTSTRRMQERWY
                     GIEYRPPAPRVEELIQLLQALWRGHAAGPFRFQGDYYDVEIETYGRAAPRRETVPIYL
                     AGVNRRMVRAAAHYADGLVGHPLYSQSWIRDAVLPSIEDGLRRSGRTRDQFVFSSEII
                     TSVSNEREEAMRAAKLQVGFYATTRTYETILAHSGWAGIADDLRAAFARRDMAGILAA
                     VPDEMVEATAVVGTPDEAREKMRRYEGTVDLAILYPPGFGLGPEESRRARDAMIETFA
                     PALTRAR"
     gene            1829..>2807
                     /locus_tag="VNL92_07165"
     CDS             1829..>2807
                     /locus_tag="VNL92_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HWO94528.1"
                     /translation="MANIEEIKAERPLLEIGKRIPEFARMGWEEIPEDDRDVRLKWYG
                     VFHRKQTPGHFMMRIRIPNGIAGAEQVRTLAGIANEFGRGAIDITTRQQVQLRWIRIE
                     NVPEIIDRLGSAGLLTLQTGMDNIRNLAGCPVAGLTPTELFDASPVVQTFNEMFVGNP
                     EFVNLPRKMNITITGCLDNCTHAETQDIALVPATKQRGSETVAGFNVLVGGKMGSGGY
                     SIAQPLAFVEPADAPRLLATIVLLFRDFGPREARNRSRLAFLIEDWGLDRFKAAVEDS
                     LERSLEPPGEDARTGTTDHIGVFRQRDDRMYYVGLLVPMGRTNGGQLLEI"
BASE COUNT          512 a          938 c          886 g          471 t
ORIGIN      
        1 ctcccagggc tcgatgacct tatcgccgta ccgaatcgtc tcgccgtcga aaacgatgag
       61 ctttacgccc tcggagacgg ctccgacgac gacaccgatg atgatggcca cgacgaccga
      121 caggacggcg atggtggtgt actggcggcg caggaacgcc atcgcgcctt cgaagatcat
      181 gctcgcgatt tcctgcatct ctggcgttcc cgggtcgcgc gcgagcacgt cccgagcaag
      241 ccagatggca aacaggatcg cgaggaggcc cgtcgcgggg acaagccaga cgaggtccat
      301 ccttcgctcc tccctcccca gcatgaggag aagtcgccgc gagaggagaa gtcacgcggc
      361 gccactcgtc ggactccatg tccgggaccg gattagcatg cggccgaagc agcagggggc
      421 cggggcgacg gctgtgccct tgcgcccgta ttctcgcttg tttcgagcgc taaggctaga
      481 ccgcgaatga atctcttcgc gcgcgcgagt cgtacaattc ccttatgtac gttctcgcgg
      541 gcatcgtggc cgccctcgcc ctcgtcgcac tgatcgtcgt tctcgcctga ccggcgccac
      601 gcctaacgag cccgtgtgag cgccggagcg aatgtctcga tcatggcgtc ccgcgcgcgc
      661 cgcgattcct cggggcccag cccgaagccc ggcggataca ggatcgccag atcgaccgtg
      721 ccctcgtaac gacgcatctt ctcgcgcgct tcgtcgggcg ttcccaccac agcggtcgcc
      781 tcgaccatct cgtctgggac ggcggcaagg atgccggcca tgtctcgccg cgcgaacgcc
      841 gctcgcaggt catctgcaat ccccgcccac ccgctatgcg cgaggatggt ctcgtaagtg
      901 cgcgtcgtcg cgtagaaccc aacctgcagc ttcgccgccc gcatcgcctc ctcgcgttcg
      961 ttggaaactg acgtgatgat ctccgaggag aagacgaact gatcgcgagt tcggcctgag
     1021 cgtcgaaggc catcctcgat cgacggcaga accgcatctc gaatccaact ctggctgtag
     1081 agcgggtgcc ccaccaggcc atcggcgtag tgcgcggcgg cccgcaccat acgccggttc
     1141 acccctgcaa ggtagatggg caccgtttcg cggcgagggg ccgcgcgccc atacgtttcg
     1201 atctcgacgt cgtagtagtc cccctggaag cggaacgggc cggcggcgtg gccccgccag
     1261 agcgcttgca gcaactggat gagctcctcg acccggggtg ccggcggtcg atactcgatt
     1321 ccgtaccatc gctcctgcat ccgtcgagtg ctcgttccga tgccgaggat gagcctgcca
     1381 tcgctcagct cgtcgatgtc catggccgcg gcggcgacca tcgcgggagc acggttgaac
     1441 gcgttcacga ttcccgaagc cacggtaagg cggctcgtat tgagggcaac cgccgtcatt
     1501 cgtacgaagc cgtccgagtt gtgaaactcc ggcacccaga tgctgtcgta ccccagctct
     1561 tcaacacgct tggcgagagc gatgagatcc gcgatgcggc cggccgcgcc cagcactccg
     1621 atcctcatga tcgccccctt tcgctcatgc tggttgtgca cggcgaacct acgggatcga
     1681 gctgcgcgtg tcaacgcgat catgcaatca agggacgatc ttcccgtcat ctgcacgaca
     1741 cgtgccctta acacgcgtgc aaccgcccgg caaacgggcc gaaacgcggg gccagtagct
     1801 tcgagacgaa gagggaggag gccgagccat ggcgaatatc gaggagatca aggcggagcg
     1861 tccgctactc gagatcggga agcgcatccc ggagttcgct cgcatgggat gggaggagat
     1921 tccggaggac gaccgcgacg tgaggctgaa gtggtacggc gtgtttcacc gcaagcagac
     1981 gcccggccac ttcatgatgc gcatccgaat accaaacggc atcgccggcg cggagcaggt
     2041 ccggaccctg gccggcatcg cgaacgagtt cgggcgcggg gcgatcgaca tcacgacgcg
     2101 acaacaggtc cagttgcgct ggatacgcat cgagaacgtc ccggagatca tcgataggct
     2161 tggctcggcc ggcctcctca ccctgcagac gggaatggac aacatccgca atctcgccgg
     2221 ctgccctgtt gccggactca cgccaacgga gctgttcgac gcgtcgcccg tcgtgcagac
     2281 gttcaacgag atgttcgtgg gcaacccgga gttcgtgaac ctgccccgga agatgaacat
     2341 cacgatcacg ggctgcctcg acaattgcac tcacgccgag acgcaggaca tcgcccttgt
     2401 gccggcgacg aagcagcgcg gcagtgaaac agtcgccggc ttcaacgtgc tcgtgggagg
     2461 gaagatgggc tccggcgggt acagcattgc tcagccgctg gccttcgtcg agccggccga
     2521 cgcgccgcgc ttgctcgcca cgatcgtcct gctcttcagg gactttgggc ctcgcgaggc
     2581 gcgaaaccgc tcccgtctgg cgtttctcat cgaggattgg ggcctcgacc gcttcaaagc
     2641 cgcggtggaa gacagcctgg aacgctcgct tgagccgccc ggcgaagacg cccgcaccgg
     2701 cacgacggat catatcggcg tgtttcgcca gcgcgacgac cgcatgtact acgtgggact
     2761 gctcgtgccc atgggccgca cgaacggagg ccagctcctc gagatcg
//
LOCUS       DATMBB010000331         2703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_134723, whole genome shotgun sequence.
ACCESSION   DATMBB010000331 DATMBB010000000
VERSION     DATMBB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134723"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VNL92_07170"
     CDS             complement(<1..704)
                     /locus_tag="VNL92_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWO94529.1"
                     /translation="MLLIRRFEDAAAEQYTRARIGGFLHLYNGQEAVAVGAIPVLRED
                     DYIVTHYRDHGQALARGIEPGRVMAELFGKETGVSRGKGGSMHLFDASRHFMGGYAIV
                     GGHLPLACGLALAAQYRGEDTAVLCFFGDGAVNEGEWHESLNLAAVWNLPVVFFCENN
                     EYAMGTAISRASAVIEVWKRTCAYDIPSERIDGMDLWAVRDATQRALAHVRAGKGPIL
                     LEAVTYRFRGHSMADP"
     gene            complement(820..2238)
                     /gene="lpdA"
                     /locus_tag="VNL92_07175"
     CDS             complement(820..2238)
                     /gene="lpdA"
                     /locus_tag="VNL92_07175"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWO94530.1"
                     /translation="MAERYDVVVLGGGPGSYTTAIRASQLGLKAALVERDRVGGLCLN
                     WGCIPSKAIIHTATLVDAVRHGEDFGILADNVRFDLGAAVDASRRVVDKMVSGVEFLL
                     ERNKVDVLHGEGRFTAADRLVVDGNEVTAKNVIVATGAEMRSIPALAVDGQTVITSRE
                     ALELRDLPPSIAIVGGGPIGLEFAYVYSAYGAEVTVIEMLPNLLPQEDEEVGKHLERV
                     MKKRGVRVMTDARVESLDRADGLVTLHVANGQREESVRAAKVLVAVGMTGRVEGYGLQ
                     EIGVEVDRGFIKVDDLGRTSVPGVWAIGDVAGPPLLAHVAAARGVAAVETIAGRTAYL
                     PPAVDMPRATYCDPEVASVGLTEAQAREQGRDVKIGKFPFSGNGRAIAQDSADGFVKV
                     VVDGQYGEILGLHIIGPAASELLGEASLARALEITAAELGATVHAHPTLAEALKEAAL
                     AVNGEAIHFWQAKSARVAAAAR"
     gene            complement(2253..>2703)
                     /locus_tag="VNL92_07180"
     CDS             complement(2253..>2703)
                     /locus_tag="VNL92_07180"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525284.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWO94531.1"
                     /translation="TVVAAKPTVYNHNIETVPRLYPTVRFKARYERSLELLRRVKELD
                     PSMTTKSGIMLGLGERRDEVRQVLRDWRANGVDLVTIGQYLRPSPRHLPVQRYVPPQE
                     FDDIAEEARALGFRGVESGPLVRSSYHAEEQAGLPAFWDGRGAARIE"
BASE COUNT          439 a          971 c          820 g          473 t
ORIGIN      
        1 tcggggtcgg ccatggagtg acctcggaac cgataggtga cggcctcgag cagaatgggc
       61 cccttgcccg cccgcacgtg cgccagggca cgttgcgtcg catcccggac cgcccacagg
      121 tccatgccgt cgatgcgttc cgatgggatg tcgtaggcac aggtccgttt ccagacctcg
      181 atgacggcgg acgcccgcga gatggccgtg cccattgcgt actcgttgtt ttcgcagaag
      241 aacacgacgg gcaggttcca aaccgccgcc aggttcagcg actcgtgcca ttcgccttcg
      301 ttcacggcgc catcgccaaa gaagcacagc acggccgtgt cttcgccgcg atactgggcg
      361 gccaacgcga ggccgcacgc cagcggcaag tgcccgccga cgatggcata cccgcccatg
      421 aaatgcctcg atgcgtcgaa caggtgcatc gagccaccct tgcctcggct gacaccggtc
      481 tccttcccga agagctccgc catcacgcgc ccgggctcga tgccacgcgc caaggcctgt
      541 ccgtggtcgc gatagtgcgt cacgatgtag tcatcctcgc gaagcacggg aatggcgccc
      601 acagcgaccg cttcctggcc gttgtacagg tggaggaacc cgccgatgcg ggcacgggtg
      661 tattgctcgg cggcggcatc ctcgaacctg cggatgagca gcatcgtgcg caggtcctgc
      721 agattgcgct cctcgacctt ctgttcgccg cttcgcctgc ctacaggacg ctcggctcgt
      781 accatcgcat tacctcgttc gccttctgct ctcgcctcgt caccgtgccg cggccgcgac
      841 gcgggccgat tttgcttgcc aaaagtggat agcctcgccg tttacggcaa gcgcggcctc
      901 cttcagtgcc tcggccaggg tgggatgcgc atgcaccgtc gcgccaagct cggcggccgt
      961 tatctcgagc gcccgcgcga ggctcgcctc gccgagcaat tcgctcgcgg ccgggccgat
     1021 gatgtggagc ccgaggatct cgccgtactg gccatcgaca accaccttca cgaatccgtc
     1081 cgcggaatcc tgggcaatcg ctcggccgtt gccgctgaac gggaacttgc cgatcttcac
     1141 gtcgcgaccc tgctcgcgcg cctgcgcctc cgtaagcccg acgctcgcga cctccggatc
     1201 gcaatacgtc gctcgcggca tgtcgaccgc cggcgggagg tacgcggtgc gaccagcgat
     1261 ggtctcgacc gccgcgacgc ctcgcgccgc tgcgacgtgc gcgaggagcg gcggccccgc
     1321 aacatcgccg atggcccaca cgccgggcac gcttgtccgg ccaaggtcgt cgaccttgat
     1381 gaacccgcgg tcgacctcca ctcctatctc ctggaggccg tagccctcga cccgtcccgt
     1441 catccctact gccacgagca ccttggccgc tcgcacggac tcctcgcgtt gcccgttggc
     1501 gacgtgcagc gtgacgagcc cgtcagcgcg atcgagggac tcgacccgcg catccgtcat
     1561 gacccgcaca ccgcgctttt tcatgacacg ctcgaggtgc ttccccacct cctcgtcctc
     1621 ttgcggaagc aggttcggca gcatctcgat gacggtgacc tcggcgccgt acgcgctgta
     1681 aacgtaggcg aactccagcc caatcggccc gccacccacg atggcgatgg acggcggcaa
     1741 gtcgcgtagc tccagcgcct cgcgcgatgt gatgaccgtt tgcccatcga cggccagggc
     1801 tgggatgctg cgcatctccg cacccgtggc tacgatgacg ttcttggccg tcacttcgtt
     1861 cccgtccacg acgaggcgat ccgccgccgt gaagcgtcct tcaccgtgaa gcacgtccac
     1921 cttgttgcgc tccagcaaga actcgacgcc actgaccatc ttgtccacga cgcgccggct
     1981 tgcatcgacc gcagcgccaa gatcaaagcg aacgttgtcg gccaggatgc cgaagtcttc
     2041 tccgtgccga actgcatcga cgagggtggc cgtatggatg atggccttac tggggataca
     2101 tccccagttc agacagagcc ctccgactct gtcgcgctcg acgagcgccg ccttcaggcc
     2161 cagctgactc gcgcgaatgg cggtcgtgta gctaccaggc cccccaccca agacaacgac
     2221 atcgtaccgt tctgccatcg gtcctccgtg cctcactcga ttcgagcggc gccgcgacca
     2281 tcccagaagg ccgggagccc cgcctgctcc tcggcgtggt acgaggagcg aacgaggggg
     2341 ccgctctcga cgccgcggaa tccgagcgcc cgcgcttcct ccgcgatgtc atcgaactcc
     2401 tggggcggga cataacgctg cacgggcaag tgcctgggtg atgggcgcaa atactgtcca
     2461 atcgtgacga ggtccacgcc gttcgcgcgc caatcgcgca acacttggcg cacctcgtcg
     2521 cggcgttccc ccaggcccag catgatgccg gacttcgtcg tcatgctcgg gtccagctcc
     2581 ttgacgcgac gaagcagctc cagcgaacgc tcgtagcgcg ccttgaatcg aaccgttggg
     2641 tacagccgcg gcaccgtctc gatgttgtgg ttgtagactg tgggcttcgc ggcgacgacg
     2701 gtt
//
LOCUS       DATMBB010000332         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_570445, whole genome shotgun sequence.
ACCESSION   DATMBB010000332 DATMBB010000000
VERSION     DATMBB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570445"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VNL92_07185"
     CDS             <1..213
                     /locus_tag="VNL92_07185"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HWO94532.1"
                     /translation="LEGLEFPAPKDEIIRVARDKGGHDSEVDQVLQSIENRSYASMDE
                     VEAEVERVYERGGGRPPAGPAASSLP"
     gene            complement(226..1122)
                     /locus_tag="VNL92_07190"
     CDS             complement(226..1122)
                     /locus_tag="VNL92_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO94533.1"
                     /translation="MDLTTILVPTDGSRTADRSLPYAAALANLMAPAEIVLLTVLPLR
                     NTGETRLPPPDHDNAEAFVRGRLAAAARRLRRRGVSTVHTEVLWNLHEDEAILAASRE
                     RDASLIVMGTHGRSGLSRALLGSTTMSVLQHAECPVVVVPPGARVGELQPRRILLPLD
                     GSPESEQALPLAQRLAGRGVRVSLLRVVPTASATTAWGGWGETYAPPTWLDEAVSAAE
                     DYLRDIAITFPPGQATIAAVIEAPAHAITQRAKEEQVDLILMTTHARSTLGRAFLGSV
                     ADRVVRSANVPVMLVRPKGTKT"
     gene            complement(1181..1564)
                     /locus_tag="VNL92_07195"
     CDS             complement(1181..1564)
                     /locus_tag="VNL92_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWO94534.1"
                     /translation="MVLLRILIVDDHDDARRALSQRLAEHKAIESVESVSSAREALLV
                     AARMAPHAVVCDVRLRVGEPVALIQSLARLDERPRIIVHAPFLLPGERELLSAAGADA
                     FVMKDAGIRALLDAVLDLNVGGMAR"
     gene            1737..>2244
                     /locus_tag="VNL92_07200"
     CDS             1737..>2244
                     /locus_tag="VNL92_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94535.1"
                     /translation="MPASTGFSLDDIAARSIDSVLAEVSSQAAALFLLDDEGGSLRLH
                     TRRGGSNALFGDLPQLLPGPLRKVEIVPGRPGSSIAFLAVPLRSRNRFCGLIAVQLTR
                     EAGISPAVRRRLQQIGDRAGELIESANLSSAIVRRNEQLQTLCEAGIAISSDLDLDVV
                     LQRVTDLAR"
BASE COUNT          371 a          738 c          765 g          370 t
ORIGIN      
        1 ttggagggcc tcgaattccc cgcgcccaag gacgagatca tccgtgttgc gcgtgacaag
       61 ggcggccatg attcggaggt cgaccaggtg cttcagtcga tcgagaaccg ctcgtacgcg
      121 tccatggacg aggtcgaggc cgaagtcgag cgagtctacg aacgtggtgg cggtcgcccg
      181 cccgcaggcc cggccgcgtc gagtttgcca taggcttggc ctgccctaag tcttcgttcc
      241 cttgggtctt acgagcatga ccggcacatt tgccgaccga acgacgcggt cggcgacact
      301 accgagaaac gcgcgtccca atgtggagcg cgcgtgggtc gtcatgagga tgagatcgac
      361 ttgctcctcc ttggctcgct gcgtgatggc atgcgccgga gcctcgatga cggcggcgat
      421 ggtggcctgg ccgggtggga acgtgatggc gatgtctcgg aggtagtcct cggcggcgct
      481 cacggcctcg tcgagccacg tcgggggagc atacgtctct ccccatcctc cccacgccgt
      541 cgtggcggag gcggtcggca ccacgcgcag caacgagaca cggacgcccc gacccgcgag
      601 gcgctgcgcc aacgggagcg cctgctccga ttcgggcgag ccatcgagcg gcaggaggat
      661 ccgccgcggc tggagctcgc cgacccgcgc gccgggcgga acaacgacga cagggcattc
      721 ggcatgttgc aggaccgaca tggtggtgct cccgagcagg gcgcgactta gaccgctacg
      781 gccgtgcgtc cccatgacga tgagcgacgc gtcacgctcg cgggacgccg cgaggatggc
      841 ctcgtcttcg tgcaggttcc acagcacctc cgtgtgcacc gtgctaaccc cccggcgacg
      901 caggcgacgg gccgcggcgg ccaagcgtcc gcgaacgaat gcctccgcgt tatcgtggtc
      961 cggcggaggc aagcgcgtct cgcccgtgtt gcgcagcgga aggacggtga gcagcacgat
     1021 ctccgcgggt gccatgaggt tggcgagggc ggccgcgtaa ggcagagacc ggtccgcggt
     1081 ccgggatcca tcggttggca ccaggatggt ggtcaggtcc atcgtcgttc cctttcggaa
     1141 gattgcatgt tgtcatgcaa gcatacggca gtgcgacaac ctatctcgcc attccgccga
     1201 cgttgaggtc cagtacggcg tcgagcagcg cgcggatgcc cgcatccttc atgacgaagg
     1261 catcggcgcc cgccgcagac aggagctcgc gctcgcctgg caggagaaac ggggcgtgga
     1321 cgattatccg ggggcgctcg tccagcctcg cgagcgattg gatgagcgcc accggttcgc
     1381 ccacgcggag ccggacatcg cagacgacgg catgcggcgc catgcgtgcc gcgacgagaa
     1441 gggcctcgcg ggcactcgag acgctctcga cggattcgat cgccttgtgt tcggcgagac
     1501 gttgggacag cgcgcgccgg gcgtcgtcgt gatcgtcgac gatgagaatg cgcagtaaca
     1561 ccatgcacat cgagcctagc gcgagggacc ggcgcagggc gcatgccgaa agtcccaagc
     1621 ttgacgggcc gagtcgcccc cgcggtccgc tttcgcgacc acccttcgtc aggatgcgcc
     1681 cggctataat gatttataca ggggttgctt ggtctggccg agaagggacg cacccgatgc
     1741 ccgcttcgac gggattcagt cttgatgaca tcgccgcgcg ctcgatagat agcgttctcg
     1801 ctgaggtctc ctcgcaggcc gcggcgctct tcctcctcga cgacgagggc gggtcgctgc
     1861 gcctacacac gaggcgcggc gggagcaacg cgctcttcgg cgacttgccg cagctgctgc
     1921 cgggtcccct gcgcaaggtg gagatcgtcc caggccgtcc cggcagcagc atagccttcc
     1981 tcgccgtccc cttacgttcg cgaaaccggt tctgcgggct catcgccgtc caactgacca
     2041 gagaggcggg catttctccg gctgtgcggc gccgtctgca acagatcggt gatcgcgcgg
     2101 gcgagctcat cgagtccgcg aacctctcgt cggcgatcgt acggaggaac gaacagctac
     2161 agaccttatg cgaggcgggc atcgccatat cttccgacct cgatctcgac gtggtcctcc
     2221 aacgcgtcac cgacctcgcc cgtc
//
LOCUS       DATMBB010000333         5959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_94215, whole genome shotgun sequence.
ACCESSION   DATMBB010000333 DATMBB010000000
VERSION     DATMBB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94215"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VNL92_07205"
     CDS             complement(<1..505)
                     /locus_tag="VNL92_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HWO94536.1"
                     /translation="MSDLPFLGDLDTGSYRFEDPWLLLAALILPALILVRLVRRPSQE
                     GLGFSRVDAIKRVGGFRRRMRWLPFALRLAAIALLVVAVARPQRGQANASVDAEGIDI
                     ALALDISSSMNETDFEQRSRLEVAQDVLRQFVDHQRDDRIGLVAFQEEARVLSPLTLD
                     HDPLVRLI"
     gene            complement(502..1551)
                     /locus_tag="VNL92_07210"
     CDS             complement(502..1551)
                     /locus_tag="VNL92_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94537.1"
                     /translation="MVFLLAIALMTLHTGPVALAGAGEGDVQVEARLTHSEATVGDRI
                     GLEIVIRAPAGSTVRVPDLPLRVGDLDLIERLPMQESDDGTEQRHRYVLSPFQTGDLF
                     VPPIRITYRLPNGTEETVETQTLPLAVRSVIPAGDPASEVRDLRSQAEVLAEPRDVPW
                     PLIGGAAGGVIASLVVLRILWLLARRLARRQAPAPPVYLTPEDEARAALDQLAANGLP
                     TRRTVKAHYATLSHIIRSYLSERHAIPALSCTTRELSVGLVERGVDRWQTRLVAGLLE
                     ECDAAHYAQYIPAKRRAEHDLSLAYEIIELARTQSEAAETAVTFGHGDNGAGGDDGAV
                     HVGGSTTAAEPERAG"
     gene            complement(1572..2510)
                     /locus_tag="VNL92_07215"
     CDS             complement(1572..2510)
                     /locus_tag="VNL92_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWO94538.1"
                     /translation="MRLLPFRGGRRPSRHASTRLGSVSPEVLRAVRRIQIRANRLLSN
                     RFLGEYHSVFKGHGIEFQETREYQPGDDERTIDWNVTARTGVPHVKKYVEERELVVLL
                     LVDVSASLNFGSVHRTKRDVAAEIAAVLALAATRNNDRVGLIAFTDRIEYYLPPRKGT
                     QHVLRLVRELLQLEPAGSGTDLAAALRFLNKVQRRRAVVFVLSDFMATGYEPALRVSA
                     LRHDVTALSLADPRELELPPVGLIELEDAESGRLVLVDASEAETRRQYRIAAIRDFEE
                     RQRTFRTIGIDEVALRTDRPYLPLLLQHFNLRARRF"
     gene            complement(2510..3499)
                     /locus_tag="VNL92_07220"
     CDS             complement(2510..3499)
                     /locus_tag="VNL92_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWO94539.1"
                     /translation="MSVDISAINERAQQQSSFVRALKSEVAKVIVGQEELIDRLLIGL
                     FCSGHILIEGVPGLAKTLAVKTLAEAIDATFVRLQFTPDLLPADLIGTTIFNTRQAEF
                     SVRKGPIFANIVLADEVNRAPAKVQSALLEAMQERQVSIGGTTYPLPQPFMVLATQNP
                     IEHEGTYPLPEAQLDRFMLKVLVNYPSQRDERQILERGLSGPPPAVHRIVRLSVIVEA
                     RAAIREVHLEDALKNYILQLILATREPAQHGLDGLSNLIAYGASPRATVYLAQAAQAH
                     AFIEGRGYVIPDDVKRVAPDVLRHRVITTYEAEAEEVSPDALIEHILERVEVP"
     gene            3588..4085
                     /locus_tag="VNL92_07225"
     CDS             3588..4085
                     /locus_tag="VNL92_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HWO94540.1"
                     /translation="MTPHVLLSILCLLLNKESDVRVDRTALRTNQGFIVSFTVLGFIL
                     GDDLGRWIVLGTGLVLALGTLAPSLALFQQFYHRVLKPAGILHPHVVAEDPAPHRFAQ
                     GIGAGFLLVAAAFLFAGLPIAGWAFSWIVTALASINLAVNFCAGCFLYFQLGKLGLLP
                     ARSSG"
     gene            4089..4511
                     /locus_tag="VNL92_07230"
     CDS             4089..4511
                     /locus_tag="VNL92_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWO94541.1"
                     /translation="MERLALALALLALVSLALVVARRFVGAQRRAGLRAAHEDATPET
                     GDRVLFFSTTTCVQCREQQAPELERLRATWRRPLVIEHVDAIEREDLARRYRILTVPS
                     TVVFAGGEPRAVNYGYASAETIAEQLAPVRLRDASARA"
     gene            complement(4508..4741)
                     /locus_tag="VNL92_07235"
     CDS             complement(4508..4741)
                     /locus_tag="VNL92_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HWO94542.1"
                     /translation="MPDHTYKIVTLVGSSDKGIQQALDSAFERASQTLRNLDWFEVKE
                     IRGAFAPDGKPGWYQVRVDVGFRVEDSADMARG"
     gene            complement(4793..5095)
                     /gene="hisI"
                     /locus_tag="VNL92_07240"
                     /pseudo
     CDS             complement(4793..5095)
                     /gene="hisI"
                     /locus_tag="VNL92_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146378.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            complement(5092..5877)
                     /gene="hisF"
                     /locus_tag="VNL92_07245"
     CDS             complement(5092..5877)
                     /gene="hisF"
                     /locus_tag="VNL92_07245"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWO94543.1"
                     /translation="MLTKRVIPCFDVDRGRVVKGVSFVDLRDAGDPVELARRYDAEGA
                     DELVFLDITASHEDRATVFDMVRRVADQVFIPFTVGGGVRTEDDVRRLLEMGADKVSI
                     NTAALDDPDLVHRAARRFGSQCIVVAIDACAVAEGTWEVYSHGGRRPTGIDAVDWARR
                     VVDLGAGELLVTSMNTDGHQRGYDLPMLRAITCAVRVPVIASGGAGEPEHLRAAIVEG
                     GADAVLAASIFHFGQYGIREVKAYLAKQGVPVRPVETSPSIGS"
BASE COUNT         1036 a         2002 c         1894 g         1027 t
ORIGIN      
        1 tgatgaggcg gacgagcgga tcgtgatcga gcgtcagcgg gctcaggaca cgggcctcct
       61 cctggaacgc cacgagcccg atgcggtcgt cgcgctgatg gtcgacgaac tggcgcagca
      121 cgtcctgcgc cacctccaac cgtgaccgtt gctcgaaatc cgtctcgttc atactggagg
      181 agatgtcgag ggcgagcgcg atgtcaatgc cttcggcatc gaccgatgcg ttcgcctggc
      241 cccgctgcgg ccgcgccacg gccaccacaa gcagggcaat tgccgcgagg cgcagcgcga
      301 agggaagcca tcgcatcctg cgccgaaagc cccccacccg cttgatcgca tcgacacgac
      361 taaatcccaa gccttcctga gagggcctgc ggacgagcct cacgaggatg agggctggca
      421 ggatgagagc ggcgaggagc aaccacgggt cctcgaagcg atagctcccc gtatccaggt
      481 cgcccaggaa cggcagatcg ctcatcccgc cctctccggc tctgcggccg tcgtcgagcc
      541 gcctacgtgc accgcgccat cgtccccgcc agcaccgttg tccccgtggc cgaacgttac
      601 cgcggtctcg gcggcctcgg attgcgttcg ggcgagctcg atgatctcgt aggcgaggga
      661 cagatcgtgc tcggcgcgac gcttcgccgg aatgtactgc gcgtagtggg cggcgtcgca
      721 ctcctcgagg agcccggcca cgaggcgagt ttgccagcga tccacgccac gctcgacgag
      781 gccaacgctc aactctcgag tcgtacacga gagggcggga atcgcgtggc gctccgagag
      841 atagcttcga atgatgtgag agagcgtcgc atagtgtgcc ttgaccgtcc ggcgggtcgg
      901 caggccattc gccgccagct ggtcgagcgc agcgcgagcc tcgtcttccg gcgtgagata
      961 cacgggcggc gccggcgcct ggcgacgagc cagccgtcgc gcaagcagcc agagaatgcg
     1021 aagcactacg agcgaggcga taacgcctcc cgccgcgcct ccgatgagcg gccacggcac
     1081 atccctcggc tcggccagca cctcggcctg ggagcgcaga tcccgcactt ccgaggccgg
     1141 gtcacccgcc ggtatgacac tgcgaaccgc gagagggagc gtctgtgtct cgaccgtctc
     1201 ctccgtgccg ttgggcaatc gataggtgat ccggatgggc gggacgaaaa ggtcgccggt
     1261 ctggaacggc gagagcacgt accggtgccg ctgctccgtg ccatcgtccg actcctgcat
     1321 cggaaggcgc tcaatcaaat cgaggtcccc aacgcggagc ggaaggtcgg gcacgcgcac
     1381 ggtggaaccc gcaggcgccc ggatcacgat ctccaggccg atgcggtcgc ccaccgtcgc
     1441 ttcgctgtgc gtgagtctag cttccacctg cacatcgccc tcgcccgcgc cagcgagcgc
     1501 gacagggcca gtgtggagcg tcatcagcgc aatggcgagc aggaacacca acctcgcgat
     1561 cgtacgcata cctagaaccg tcgagctcgc aggttgaagt gctggagcag cagcggcaga
     1621 tagggacgat cggttcggag cgccacctca tctatgccga tcgtccgaaa ggtccgctgc
     1681 cgttcctcga agtcccgtat cgccgcgatg cggtactgcc ggcgcgtctc ggcctccgac
     1741 gcgtcgacga ggacgagacg cccgctctcc gcatcctcca gctcaatcag tcccacgggt
     1801 gggagctcca gctcacgcgg gtcagcgagc gacagcgccg tcacgtcgtg ccggagcgcc
     1861 gagacgcgga gcgccggctc gtagcccgtg gccatgaagt cggagagcac gaacacgact
     1921 gcgcggcgcc tctgcacctt gttgaggaac cgcagcgcgg ccgcgaggtc ggtcccggaa
     1981 ccggccggct ccagctgcag taactcccgc acgagacgga gcacgtgctg cgtacccttc
     2041 ctgggtggca ggtagtactc aatgcgatcc gtgaaggcga tgaggccaac gcggtcgttg
     2101 ttgcgcgtcg cggcgagggc cagcacggcc gcgatctcgg cggcgacgtc acgcttcgtc
     2161 cgatgaacgg agccgaagtt gagagaggcg ctcacatcca cgagaagcag gacgacgagc
     2221 tcgcgctcct ccacgtactt cttcacgtga ggcactccgg tgcgggcggt cacgttccag
     2281 tcgatggttc gctcgtcgtc gccgggctga tactcccgcg tttcctgaaa ctcgatgccg
     2341 tggcccttga agacactgtg gtactcgccg agaaagcgat tcgaaaggag gcggttggcc
     2401 cgaatctgaa tacgacggac agcccgcaag acttcggggg agacgctgcc gagacgcgta
     2461 gaggcgtgcc tggaaggacg ccgcccgccc cggaacggaa ggaggcgcac tacggcacct
     2521 ccacgcgctc cagaatgtgc tcgatgagcg cgtccggcga aacctcctcc gcttccgctt
     2581 cgtaggtggt gattacgcgg tggcggagca catcgggcgc aacacgcttg acgtcgtccg
     2641 ggatcacgta gcctcgtccc tcgatgaagg cgtgcgcctg cgccgcctgg gcgagataca
     2701 cggtagcccg gggcgaggcg ccatacgcaa tcaggttgct aagcccatcc agaccatgct
     2761 gcgccggctc ccgggtcgcc agaatcagtt gcaggatgta gttcttgagc gcgtcctcca
     2821 gatgcacctc gcggatggcc gcccgcgctt cgacgatgac actgagccgg acgatgcgat
     2881 gcacggccgg cgggggtccg ctcagcccac gctcgaggat ctgacgttcg tcacgctggc
     2941 tcggatagtt gacgaggacc ttgagcatga accggtccag ttgcgcctcg gggagggggt
     3001 aagtgccctc gtgctcgatc gggttctggg tcgcgagcac catgaacggc tgcggcagcg
     3061 ggtacgttgt cccgccgatg ctcacttgcc gctcctgcat cgcctcgagg agcgccgact
     3121 gcaccttggc gggcgcgcgg ttcacctcat cggcgaggac gatgttggcg aagatggggc
     3181 ccttgcgaac cgagaactcg gcctgccgcg tgttgaagat cgtcgttccg atcaggtcgg
     3241 ccggcaacaa gtcgggcgtg aattgcagac ggacgaacgt ggcgtcgatg gcctccgcca
     3301 atgtcttgac ggcgagcgtc ttcgcgaggc cgggcacacc ttcgatgagg atgtgccccg
     3361 agcagaagag cccgatgagc aggcgatcga tcagctcttc ttgtccgacg atgaccttgg
     3421 cgacttcgct cttgagagcg cgcacaaagc tgctttgttg ttgggctcgt tcgttgatcg
     3481 cggagatatc gaccgacacg ccgcctcccg cactcccgcc gatgcgacct tagtctatcc
     3541 gagcacgatc ggccgagcaa gcaaatgtca aagacgatgc cgattcgttg acgccccacg
     3601 tattgttgag tatcctttgc ttattactca acaaggagtc ggacgtgcgg gtcgaccgga
     3661 ctgccctccg gacgaatcag ggcttcatcg tctccttcac cgtcctcggc ttcatcctcg
     3721 gcgatgacct gggccggtgg atcgtgcttg gcactggcct cgtgctggct ctgggcacgc
     3781 tcgcaccatc gctcgccttg tttcagcagt tctatcatcg cgtgctcaaa cccgcgggca
     3841 tcctccatcc gcacgtcgtc gccgaggacc cggctcccca tcggtttgcc cagggtattg
     3901 gcgccggctt cctgcttgtc gccgccgcgt tcctcttcgc gggccttcct atcgccgggt
     3961 gggcgttctc ctggatcgtg accgcgctcg cctcgatcaa tctcgcagtg aacttctgcg
     4021 ccgggtgctt cctgtacttc caactgggga agctaggttt gcttccagcc agaagctccg
     4081 gctgacctat ggagcgcctc gccctggctc ttgcgttgct ggcgctggta tcgcttgccc
     4141 tcgtcgtggc acgcaggttc gtcggcgccc agcggcgagc gggcttgcgc gcagcacatg
     4201 aggacgccac gcccgagacg ggcgatcgcg tgctcttctt ctccaccacg acctgtgtcc
     4261 aatgccgtga acagcaagcg cccgagttgg agcgccttcg ggcaacgtgg cgacggccgt
     4321 tggtcatcga gcacgtcgat gctatcgaac gcgaggacct tgcacggcgg tatcgcattc
     4381 tcacggtgcc aagcaccgtc gtgtttgccg gcggcgagcc gcgtgcggtc aactacggct
     4441 acgcctcggc ggaaacgatc gcggaacagc tggcgccggt tcgcctgcgc gatgcttcgg
     4501 ctagggccta gccccgggcc atatcggcgc tgtcctccac gcgaaagccc acgtccaccc
     4561 gcacctgata ccagccgggc ttcccatccg gtgcgaaggc gccacggatc tccttgacct
     4621 cgaaccagtc aaggttgcgc aacgtctgcg aggcgcgctc gaaggcgcta tccaacgctt
     4681 gctgaatccc cttgtccgag gaaccgacga gcgtgacgat cttgtaggtg tggtccggca
     4741 tgctgaaatc ctctctcctt taggcgcccg tccgggcaac ctcgggccac tcgcgaaaga
     4801 agcatgaacg ctcacctgtg tgacacgtgg gaccagccgg ctcaactcgc acgaggatcg
     4861 cgtcggcatc gcagtcggtg cgaatcgcgc gcacgtggag cacgtttccg gaggtcgcac
     4921 ctttgtgcca caactcgccc cgcgatcggc tccagaacca cacttggccc gacgtcacgg
     4981 ttcggcgaag cgattcctcg ttcatgtagc ccagcatcag aacctcgcca gtggcgtcat
     5041 cctggatgat ggcaggaatg agaccgtctt ccttgaatgc cagttccacc gtcagctccc
     5101 aatcgacggc gaagtctcga cgggccgaac aggcacgccc tgcttcgcga gataggcttt
     5161 gacttcccga atgccgtatt gcccgaagtg gaaaatgctc gccgccagta ccgcgtcggc
     5221 gccgccttcc acgatggccg ctcgcagatg ctccggctcg cccgcgccgc cgcttgcgat
     5281 gacgggcacg cgcactgcac acgttatggc ccgcagcatc ggcaagtcgt aaccgcgctg
     5341 gtgtccgtcg gtgttcatgc tcgttaccaa cagctcccct gcccccaagt cgacgacacg
     5401 gcgagcccaa tcgactgcgt cgatgccggt agggcgacga cccccatgcg agtacacctc
     5461 ccatgttccc tcagcaacgg cgcaggcatc aatggcgacg acgatgcatt ggctcccaaa
     5521 tcgccgcgct gcccggtgca cgaggtcggg atcgtcgagg gctgcagtat tgatcgatac
     5581 cttgtcggcg cccatctcga gcaggcggcg aacgtcgtcc tccgtgcgca cgccgccacc
     5641 gaccgtgaac gggatgaaca cctgatcggc gacccggcgc accatgtcga acaccgtcgc
     5701 gcggtcctca tgcgaggccg tgatatcgag gaagacgagc tcgtcggcgc cttcggcgtc
     5761 gtaacggcgg gcaagctcga ccggatcgcc cgcgtcccga aggtcgacga acgagacgcc
     5821 cttcaccacg cggccgcggt ccacatcgaa gcagggaatc acacgctttg tgagcattaa
     5881 ggttagcgta cgcggcggac agcagcctgt ccaccgtcct ccgcttacct acgccgaccg
     5941 ggcacgcatc gcatcgagc
//
LOCUS       DATMBB010000334         7094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_144456, whole genome shotgun sequence.
ACCESSION   DATMBB010000334 DATMBB010000000
VERSION     DATMBB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144456"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="VNL92_07250"
     CDS             <1..146
                     /locus_tag="VNL92_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWO94544.1"
                     /translation="QNLVSSFGSNYQVLTAAAFVSMAVPLAIFFALQRYFVQGILAGA
                     VKA"
     gene            171..1313
                     /locus_tag="VNL92_07255"
     CDS             171..1313
                     /locus_tag="VNL92_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94545.1"
                     /translation="MATVRFDKVTKRFGETVAVRELDLDIPDREFLVLVGPSGCGKST
                     TLRMLAGLESVTEGRIYIGDRDVTNVRARDRDIAMVFQSYALYPHMSVYDNMAFALTL
                     RKTSKDEVRRRVEEVARILGIEELLQRKPGQLSGGQRQRVALGRAIVRQPAVFLLDEP
                     LSNLDAKLRVQTRAEISKLHRRLATTFIYVTHDQIEAMTMATRIAVMNNGSLQQVGPP
                     DVLYDHPRNLFVAGFIGSPAINTFSGTVVRAGEELRIDTGSFAIPIRTKASALSAYLG
                     KQVIFGVRPEHVHNLAYLPPGIESATVPAEVDVAELLGNEVMLHLVLPGNARCVARVD
                     PRTRARPGQHIEVGFDLAQLHVFDPQSELAIAETAPAVPSIGTASS"
     gene            complement(1383..2468)
                     /locus_tag="VNL92_07260"
     CDS             complement(1383..2468)
                     /locus_tag="VNL92_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWO94546.1"
                     /translation="MKAAIFHGPNQPLTIEDNVEVDKPMRNEVLVRTVASGVCHSDLH
                     FVDGLYPYPAPAILGHESAGIIEAVGEDVTYVKPGDHVIACLSVFCGKCEQCLTGHPN
                     RCQSPDVRRGMEDPPKYTWKGQPVFQFLNISSYAEKMLLHENAVVKIPEDYPLDRAAL
                     IGCGVTTGVGAALYTAKVEPGSTVAVFGCGGVGLSVIQGAYIAGARQIIAVDMVESKL
                     GMAREFGATHTVDASGRDPVQAIQEMTGGGVDYSFEAIGVKRAAEQAYEALRPGGTAT
                     VIGMIPVGQKIEIDGFSLLGEKRLQGSVMGSNRFRVDMLKYIEFDRQGRLHLDKMITR
                     RGKLEDVNEAFRAMKAGEVARTVLMIE"
     gene            2669..4105
                     /gene="pyk"
                     /locus_tag="VNL92_07265"
     CDS             2669..4105
                     /gene="pyk"
                     /locus_tag="VNL92_07265"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWO94547.1"
                     /translation="MPTFAQQRRAKIICTLGPASDDPDTIRMLIQRGMNVARINMSHG
                     THERHRETVRLVREAAEHFGQPIAVIGDLQGPKIRTGTLEQGKVELQPSASFSLTTEP
                     VVGDDHRVSINYPDIARDVGPGDRILVSDGMIELKVEEARPSEVATTVVHGGLLLDHQ
                     GVNLPDGASALSSLTEKDLVDLDFLLDLPVDYVALSFVRRAQDLRDLRWRMGQRGAKT
                     PIIAKIEKRQALENLDEVLTMADALIVARGDLGVELPPEEVPLWQKRIVARAGQYLVP
                     VITATQMLESMVQSPRPTRAEASDVANSVWEGADALMLSGETAIGGYPVEAVDMMDRI
                     IRAAESLSTPDARPISPKVMEYSYDIARAARQIAEANHEVRAIVAFTQSGYTARLISK
                     ERPPVPILALTPSEDVRRRMALYWGVLARECRPLTSIDDMLVEVDHVARSHYALREGA
                     TVIIVGSLPVERQGMTNFLKLHRIGELD"
     gene            4331..6406
                     /locus_tag="VNL92_07270"
     CDS             4331..6406
                     /locus_tag="VNL92_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWO94548.1"
                     /translation="MSAIGRDEEFRALVEQASDIIMTIGGDGVIHFASPATERILGYP
                     ADTLVGSQVLDLLEPSDRHTLIAALADLEPGNHLAMRLRAAHISGQWRTLDIRMTLTA
                     AKKGQRAFVVTARDVTEEADLHDAIRARESHFRALLQNASDMIISLRPDFTVGYASPS
                     VRTVIGYDPEELRGKPIAELLHPADVASLAEERKRELRPGETMTIEVRVRHALGAWRF
                     IEAKLSSLPSSGDAGGDVLVINGRDITERKTYEDAITHHTFHDPLTNLPNRILLADRL
                     QQAVARSERQGRAVAVLLLDLDRFKLVNDSLGEETGNALLMTVAERLVQVTPPDATVA
                     RAGGDEFAVVLGGLTSIGDAVNAAQQIREQLAKPVLLDGREVFVQLSIGIALSGGDGG
                     EQRDLLRNAGIALHRAKANGRGRTEIFTANVDTEALHRLTLEGDLRHAVQRHELLLLF
                     QPVMRLKSASPVGMEALLRWRHPEHGLVPPLDFIPLAEETGLIVDIGQWVLREACKQA
                     HAWQQRFPDANPSVSVNVSAREFQDPYLVAKVRDVLRGAKLRPDSLILELTESSLLAD
                     VANATRTLDALKGLGVRIALDDFGTGYSSMSYLKKLPVDILKVDKTFIDGLGKDLRDT
                     AIVRAIVEIARSLDLQVTAEGIEDAAQVTQLRILGCEFGQGYYFAKPLDQRQASAFQS
                     GSRGLRAAS"
     gene            complement(6428..6805)
                     /locus_tag="VNL92_07275"
     CDS             complement(6428..6805)
                     /locus_tag="VNL92_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94549.1"
                     /translation="MQGMEYVARLEDTVAKRRDKFVVTRAMLESAAAHAAARSLPVVE
                     GFREPVLRVGFATAFRVEEEGTEAVLLCDVVLEVEPRGRVLAPSVRARGEEWTIQFLR
                     LVEQHKNPRASLRPSQGDDASTS"
BASE COUNT         1280 a         2408 c         2175 g         1231 t
ORIGIN      
        1 ttcaaaacct cgtgagctcg ttcggaagca actaccaagt actaaccgcc gcggccttcg
       61 tctcgatggc cgtgcccctc gccatcttct ttgcgctgca gcgctacttc gtgcagggca
      121 tcctggccgg ggccgtgaag gcgtaagcag cgcgggtaag gagcttctcg atagcaacgg
      181 tgcgctttga caaggtcacg aagcgcttcg gcgaaaccgt cgccgtgcgc gagctcgacc
      241 tcgacattcc ggatcgcgag tttctcgtcc tcgtcgggcc ctcgggttgc ggtaagtcca
      301 caaccctacg gatgcttgcc ggactcgaaa gcgtcaccga aggtcgcatt tacatcggcg
      361 accgcgacgt cacgaacgtg cgcgcgaggg accgcgacat cgctatggtc ttccagtcgt
      421 acgcgctcta tccgcatatg agcgtgtacg acaacatggc ctttgccctc accctacgga
      481 agacgtcgaa ggatgaggtt cggcgccggg tagaggaggt ggcgcgcata ctggggatcg
      541 aggagttgct ccagcggaag cccgggcagc tctctggcgg acagcgtcaa cgggtagcgt
      601 tgggccgcgc catcgtgcgt cagcccgccg tcttcctgct cgatgagccc ctctcgaatc
      661 tcgatgccaa gctgcgtgtg caaactcggg cggagattag caagcttcat cggcgtctcg
      721 ctaccacgtt catctacgtg acccacgacc aaatcgaggc catgacgatg gccacacgca
      781 tcgcggtcat gaacaacggt tcgctccagc aagtgggccc gcccgacgtt ctctatgatc
      841 atccacgcaa tctgttcgtc gccgggttca tcggaagccc ggccatcaac accttctccg
      901 gaaccgtcgt ccgcgcgggc gaggagctgc ggatcgatac cggctcgttc gcaataccca
      961 tacgaacgaa ggcaagcgcc ctctccgcct acctcggcaa gcaggtcatt tttggtgtgc
     1021 gtccggagca tgtgcacaac ttggcgtacc tgccgcccgg cattgaatca gcgactgtcc
     1081 ccgccgaggt cgacgtcgcg gagttgctgg ggaacgaggt gatgcttcac ctcgttctgc
     1141 cgggcaacgc gcgttgcgtg gcgcgcgtcg acccgcgcac tcgggcccga ccaggacagc
     1201 acatcgaggt aggcttcgac ttggcccagc tgcacgtctt cgacccgcaa tccgagctag
     1261 ccatcgctga aacagccccg gccgtgccga gcatcggaac ggcgtcaagc tgaggcaaat
     1321 cgcacggtat cgaggacacg cggttctgga gggcagcagt agcgtggcct tgcgcgatgg
     1381 ggctactcga tcatgagcac cgtgcgcgcg acctcgccgg ccttcatcgc gcggaaggcc
     1441 tcgttcacgt cctccaactt gccgcgccgc gtgatcatct tgtccagatg cagccgcccc
     1501 tgccggtcga actcgatgta cttcagcata tcgacgcgga agcggttcga gcccatgaca
     1561 ctgccctgaa gccgcttctc ccccaacaag ctgaagccat cgatctcgat cttctggccc
     1621 accgggatca tgccgatgac ggttgcggtg ccgcccggcc gcagcgcctc ataggcttgc
     1681 tccgcggcgc gcttcacccc gatcgcctcg aaggagtagt ccacgccgcc gcccgtcatc
     1741 tcctggatgg cttgcaccgg gtcgcgtccc gacgcgtcaa ccgtgtgggt tgctccgaac
     1801 tcgcgcgcca taccgagctt gctctccacc atgtccacgg caatgatctg gcgggcgccc
     1861 gcgatgtagg cgccttggat gaccgacaga ccgacgccgc cgcatccgaa cacggcaacc
     1921 gtcgagcccg gctccacctt ggccgtgtat agggcagcgc cgaccccggt cgtcacgccg
     1981 cagccgatga gggccgcgcg gtccagcggg taatcctccg ggattttgac cacggcattc
     2041 tcgtggagca gcatcttctc cgcgtaggaa gagatattga ggaactgaaa gaccggctgg
     2101 cccttccacg tgtacttcgg tggatcttcc atacctcggc gcacatctgg gctctggcat
     2161 cgattcgggt ggccggtgag gcactgctcg cacttcccac agaagacgga gaggcaggcg
     2221 atgacgtggt cgcccggctt tacgtaggta acgtcctcgc cgaccgcctc gatgatgcct
     2281 gccgactcgt gtccgagaat agcgggagca ggatacgggt agagcccgtc gacgaagtgg
     2341 aggtcgctgt ggcaaacgcc gctcgcgacc gtgcggacta ggacctcgtt ccgcatcggc
     2401 ttgtcgactt cgacgttgtc ctcgatcgtg agcggctggt ttggcccgtg aaagatggcc
     2461 gccttcatag caaatcccct cgtttcataa tctcgcacgt gcaggtcgcg cggagcgccg
     2521 cgaacataca ccgggtcgca aggcgcgtca agaagctgtg cgcgtttatt caccagccag
     2581 gacgcgcttt ccgcttgctc taaccgtggg gcgcggcgtt atcctaaaga gagccgcgca
     2641 tatatgggcc acgatgcccg gggaccaaat gcccaccttc gcccagcaac gccgagctaa
     2701 gattatctgt accctcggac ccgcgagcga cgacccggac acgatccgca tgctgattca
     2761 gcgcggcatg aacgtcgccc gcatcaacat gtcgcatggc acccacgagc gtcatcgcga
     2821 aaccgtgcgc ctggtgaggg aggcggccga gcatttcggc cagccgatcg ccgtcatcgg
     2881 ggatctgcaa ggccccaaga tacgcacggg tacactcgag cagggcaagg tcgagttgca
     2941 gccatcagcg agcttcagct tgacgacgga accggtggtt ggcgacgacc accgcgtatc
     3001 gatcaactac ccagacatcg cccgcgatgt tggccccggc gatcgcattc tcgtctccga
     3061 cgggatgata gagctcaagg tcgaggaggc gcggccatcg gaggtggcga caaccgttgt
     3121 gcacggaggc ctcctgctcg accatcaggg cgtgaacctc cccgatggcg cgagcgccct
     3181 ctcctccctt acggagaagg acctcgtcga cctcgatttt cttcttgatc tgcccgtgga
     3241 ttatgtcgcc ttgagcttcg tccgtagggc gcaggacctt cgcgacctgc gctggcgcat
     3301 ggggcaacgg ggcgccaaaa cgcccatcat tgccaagatc gagaagcgcc aggccctcga
     3361 gaacctcgat gaggtcctga caatggcgga tgcactcata gtcgcccggg gcgatttggg
     3421 cgttgagctg cccccagagg aggtgcccct ctggcaaaag cgcatcgtcg cgcgagccgg
     3481 ccagtacctt gtgccggtca tcaccgccac gcagatgctc gagtcgatgg tccagagccc
     3541 tcgccccact cgcgccgagg cgagcgacgt cgcgaactcc gtgtgggagg gagccgatgc
     3601 cctcatgctg agcggggaga ctgcgatcgg tgggtaccca gtcgaagccg tcgacatgat
     3661 ggaccgcatc atccgggccg ccgagtcgct gtccacaccg gacgcgcgtc ccatctcccc
     3721 caaggtcatg gagtactcgt acgacatcgc ccgtgccgcc cgtcagattg cggaagccaa
     3781 ccacgaggta cgtgccatcg tcgccttcac ccaaagcggc tacacggccc ggctcatctc
     3841 gaaggagcgg ccccctgtac ccatcctcgc actcacgcct tccgaagacg tccgccggcg
     3901 catggccctc tactggggcg tccttgcacg ggaatgccgt cccctgacca gcatcgacga
     3961 catgctcgtc gaggtcgacc acgtggcacg atcgcactac gctctgcgcg agggcgcgac
     4021 cgtcatcatc gtcggcagtc tgccggtcga acggcaaggc atgacgaact tcctgaaact
     4081 tcaccgcatc ggcgaattgg attaggggcg gaccaatatc cccgcatttc tcttcactcc
     4141 gcgcacgtct gtccgatact tgttaaggta gtgcacgggg gaagcgaacg atgtgctcct
     4201 gccagcacgc gcgtcttgat ttccacgacg gatcgatctc tgcacgttcc gctcgagtgc
     4261 cgcgccaagt cggcttcgtt gacgacgctt cggcagcgcg gaacgcgcgc ctcccgggag
     4321 gtcgaaccct gtgagcgcaa tcggccgcga cgaggaattc cgggcgctcg tcgagcaggc
     4381 gtcggacatc atcatgacga tcggcggtga tggcgtcatt cacttcgcca gccccgcgac
     4441 cgaacggatc ctgggctacc cggccgacac gctcgtcggc tcccaagtgc tggacctgct
     4501 tgagccttcg gaccgccaca cgttgatcgc tgcgctcgcg gacttggaac ccggcaacca
     4561 cctagccatg cggctgcgag ccgcccacat ctcgggccag tggcgcacgc tcgacatccg
     4621 gatgactctt actgcggcca agaagggcca gcgagccttc gtcgtcaccg cgcgagacgt
     4681 gaccgaagag gccgacctcc acgatgccat ccgcgcccgc gaatcgcact tccgggccct
     4741 cctccagaac gcgtccgaca tgatcatctc gctgcgcccc gatttcaccg tcggctacgc
     4801 aagcccatcg gtgcgaaccg tcatcggata cgatccggag gagcttcgcg gcaaaccaat
     4861 cgctgagctg ctgcatcccg ccgatgtcgc cagcctggct gaagaacgca agcgcgagtt
     4921 gcgcccgggc gaaaccatga ccatcgaagt gcgtgtccga cacgccctcg gcgcctggcg
     4981 gttcatcgaa gcgaagctca gctcgctccc ctcgtcgggg gacgcgggcg gagacgtcct
     5041 cgtcatcaac gggcgcgaca ttacagaacg aaagacgtac gaggacgcga tcacgcatca
     5101 cacgttccac gacccgctga ccaatctgcc gaatcgcatc ctcctcgccg accggctgca
     5161 gcaggccgtg gcccgttccg agcgccaggg gcgcgctgtg gccgtgctgc tgctcgacct
     5221 cgacaggttc aagctcgtca acgatagcct cggcgaggag accggcaacg ccctgctcat
     5281 gactgtggcc gaacgcttgg tccaggtcac cccacccgat gcgacggtcg cgcgcgccgg
     5341 aggtgatgag ttcgcggtcg tgctgggtgg gctcacaagc atcggggacg cggtgaatgc
     5401 ggcgcaacag atccgcgaac agctcgcgaa gcccgtcctc ctcgatggac gcgaagtctt
     5461 cgtgcagttg agtatcggta tcgctttgag cggcggcgac ggcggggagc agcgcgatct
     5521 gcttcggaat gccggaatcg cgctccatcg ggcaaaggcg aacgggcgcg gccgcaccga
     5581 gattttcact gcgaacgtcg acacggaagc cctgcaccgg ctcacactcg aaggggacct
     5641 gcgtcacgcc gtgcagcggc acgagttgct tctcctgttc cagcccgtca tgcgcctcaa
     5701 gtcggccagc ccggtcggga tggaggcgct gctccgctgg cggcatccgg agcacggact
     5761 ggtgccaccg ctcgatttca tccccctggc cgaagagaca ggactcatcg tggacatagg
     5821 ccagtgggtg ctgcgcgagg cgtgcaagca ggcacacgcg tggcagcagc gctttcccga
     5881 tgcgaaccca tccgtgagcg tcaacgtttc cgcccgcgag ttccaggatc cctatctcgt
     5941 ggccaaggtt cgcgacgtgt tgcgaggtgc caagctccgg ccggactcgc tcatcctgga
     6001 gctgacggaa agcagcctcc tcgccgacgt ggcgaacgcc acgcgcacgc tcgacgcact
     6061 caagggcctc ggcgtccgca tcgcactcga cgacttcggt acgggttatt cctcgatgag
     6121 ctacctgaag aagctgccgg tcgacatcct gaaggtggac aagacgttca tcgacggcct
     6181 cgggaaggac ctgcgcgaca cggcgattgt gcgcgccatc gtggagatcg cccgcagcct
     6241 cgacctccag gtcacagccg aaggcatcga ggacgccgcg caggtcacgc agcttcgcat
     6301 cctcggttgc gagttcgggc aagggtacta cttcgcgaag cccctcgacc aacgacaggc
     6361 gtcagcgttc cagtcgggca gccggggtct gcgggcggcg agctaagcta gccaggtccg
     6421 ccccacgtca ggacgtcgac gcgtcgtccc cctgactcgg ccgaagcgat gcgcgcggat
     6481 tcttgtgctg ctcgaccagc ctgaggaact gaatcgtcca ctcctcgccg cgcgcccgca
     6541 cggatggggc gagcacacga ccacgcggct ccacctcgag cacgacatcg cacaagagaa
     6601 cggcctcggt gccttcctcc tccacgcgaa acgccgtggc gaatccgacc ctgagcaccg
     6661 gctcgcggaa cccctcgacg acgggaagag agcgggctgc ggcgtgtgct gccgccgact
     6721 ccagcatcgc gcgcgtcacg acaaacttgt cgcgccgctt cgcaacggtg tcctcgaggc
     6781 gcgccacgta ctccatgccc tgcacacgtc aatcctagcc tgaactcgag cgtcgcgttc
     6841 gttggccttc aacgcgaggc gtgcgtacaa tgatgcacgc gccactcggc gtacacctcc
     6901 gcgaagggcc aacgcctccg tgcgacagcc gtactggatt atcctcttcg tcgccgcgct
     6961 cgcaataccg ggcggcgtac tgcgttttag cggggccgac ctccctgtcg gcctcgcaac
     7021 gctcgtcttc ggcgtggccg tcttttcggt ggccttcctc ctcacctgga cggcggaagc
     7081 agccgagacg gagg
//
LOCUS       DATMBB010000335         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_55077, whole genome shotgun sequence.
ACCESSION   DATMBB010000335 DATMBB010000000
VERSION     DATMBB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55077"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VNL92_07280"
     CDS             complement(<1..203)
                     /locus_tag="VNL92_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWO94550.1"
                     /translation="MIARRALVEQLAIDGVSHVFGNPGTTEQGFMDILQDYPQIQYVL
                     ALHESGAVGMAEGYARATGKPAFV"
     gene            complement(301..975)
                     /locus_tag="VNL92_07285"
     CDS             complement(301..975)
                     /locus_tag="VNL92_07285"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWO94551.1"
                     /translation="MRAIHRTGLTTAAFLILVVSASAPIVIQRTTAAPQELSAGWNMV
                     VYEGPALPPDEAFASLGDALTGVYLQVDADSWLSYVPAFPDAYNSLTLVEPDNVYWLY
                     LSAPATLHPPQRTIVQAVSGLAGSWMQVERGKAVFYGWELAGNRTACGPIFDPTKLTA
                     ASNTLPCGTVARVTNVANGKSVIVTVNDTGGFRPPIVIDLSKAAYETIAPPNSGEITV
                     TIEVQQ"
     gene            complement(1066..>2054)
                     /locus_tag="VNL92_07290"
     CDS             complement(1066..>2054)
                     /locus_tag="VNL92_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HWO94552.1"
                     /translation="ELSEYLGIVPRHVDGTLVGGCSFMILTRHAAAAIKAGLCDVALV
                     THGESGRSRVGARGFGGSPQSPQGQFEMPYGVFGPPSMFPMGVARHMAKYGTTSEQLA
                     AVAVSTRQWAMKNPRAMMRDPITIEDVLNSRIICWPMHLLDCCLVTDGGGALVIVSAE
                     RAQSMGLGHPPVYLLGAGEATEHVMISMMADMTSSAAYRKSGEEAFKMAGVTHKDIDH
                     LMFYDAFTHTPLYAIEDLGFVKKGEGGPWMAEMHGAPGGDLPLNTNGGGLSYTHTGMY
                     GMFAIQEACRQLRGEGGERQVPNVKISLVHGPGGMFSAASTLILTNEPPTAA"
BASE COUNT          355 a          645 c          678 g          376 t
ORIGIN      
        1 acgaaggcag gcttgccggt cgcgcgggcg tagccttccg ccatgcccac cgcccccgat
       61 tcgtgcaggg ccagtacgta ttgaatctgg ggatagtctt gcaggatgtc catgaacccc
      121 tgctcggtgg tgcccgggtt cccgaacacg tggctgacgc catcgatagc gagctgctcc
      181 acgagcgcac gccgagcgat catccgtgac actccggcct ccttcggaca caacctggcc
      241 cggccgaata tggtgttcgg ccgggccaat catacgacag ggactatcgg ccgtgaagaa
      301 ctactgttgc acttcgatgg tcacggttat ctccccggag ttcggcggcg cgatcgtctc
      361 ataggccgcc ttcgagagat cgatgacgat gggcgggcgg aagccgccgg tgtcgttgac
      421 cgtcacgatc accgacttgc cgttcgcgac attcgtgact cgcgccaccg tgccacacgg
      481 gagcgtgttg ctggcggccg tcagctttgt cggatcgaag atgggcccgc acgcggttct
      541 gttgccggcc agctcccagc cgtagaacac ggccttcccc cgctccacct gcatccagga
      601 cccggcgagc ccgctcacgg cttggacgat tgtccgctgc ggcggatgga gcgttgcggg
      661 cgcggacaga tacagccagt acacgttgtc cggctccacg agggtcagtg agttgtacgc
      721 gtcggggaag gccgggacgt aggacagcca ggagtcggcg tccacttgga gatagacgcc
      781 ggtcaacgca tcgccgaggg aggcgaacgc ctcgtcggga gggagtgccg gtccttcgta
      841 gacgaccatg ttccagccgg cgctcagctc ttggggcgcg gctgtggtcc tctgaatgac
      901 tatgggtgcg gaagcggaga caactaggat gagaaatgcg gctgttgtga gaccggtacg
      961 gtgaatggca cgcaataagc acctccttga actcgaaacg tacgaacgcg gcccagacta
     1021 tgggccgcgt tccttaaggt caaatctgct tctgtaagcg aacggtcagg cagcggtcgg
     1081 cggttcgttc gtgaggatta atgtcgacgc agctgagaac atgccgcccg ggccgtggac
     1141 aaggcttatc ttcacgttcg gtacctgccg ttcgcctccc tcgcctcgca gctgccggca
     1201 tgcctcctga atggcgaaca tcccgtacat cccggtgtga gtgtaagaga ggccgccgcc
     1261 gttggtattg agcggcaggt cgcctccggg cgcgccgtgc atctcggcca tccacggccc
     1321 gccttcgccc ttcttgacga agcccaggtc ctcgatggcg tagagcggcg tgtgcgtgaa
     1381 ggcatcgtag aacatgagat ggtcgatgtc cttgtgggtc acgcccgcca tcttgaacgc
     1441 ctcctcgccc gatttgcggt acgccgcgga ggaggtcata tctgccatca tgctgatcat
     1501 gacgtgctcg gtcgcctcgc ccgcgcccag gaggtagacc ggcgggtgcc cgaggcccat
     1561 cgattgggcg cgctcggcgg acacgatgac gagggctccg cccccgtccg tgacgaggca
     1621 gcagtcgagc aggtgcatcg gccagcagat gatgcgcgaa ttcaggacgt cctcgatcgt
     1681 gatcggatcg cgcatcatcg cgcggggatt cttcatggcc cactgccgag tggaaacggc
     1741 aacggccgcg agctgttcgc tcgtcgtccc gtacttggcc atgtggcggg ctacacccat
     1801 cgggaacatc gagggtgggc cgaagacgcc gtagggcatc tcgaactggc cctgggggga
     1861 ttgcgggctg ccaccgaatc ctcgcgcgcc cacgcgcgag cgtccgcttt cgccatgggt
     1921 cacgagggcc acatcgcaca agcccgcttt gatggcggca gcggcgtggc gggtcaggat
     1981 catgaaggag catccgccta cgagcgtccc atcgacgtgg cgcggcacga tgcccaggta
     2041 ttcgctcagc tccg
//
LOCUS       DATMBB010000336         2978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_161146, whole genome shotgun sequence.
ACCESSION   DATMBB010000336 DATMBB010000000
VERSION     DATMBB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161146"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..936)
                     /gene="uppS"
                     /locus_tag="VNL92_07295"
     CDS             complement(130..936)
                     /gene="uppS"
                     /locus_tag="VNL92_07295"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWO94553.1"
                     /translation="MRQKTSTIVAERRDKAQAENLDPALPEHVAVIMDGNGRWASQRG
                     LPREEGHSAGTENIRQVIEAFANRGVRYLTLYAFSTENWGRPQSEVDALMHLLDTVIE
                     REVPELNRLGIRVVHIGRLDDLSDDLQRRVREAMDLTSKNDRMTVAIAFNYGGRREIV
                     DAVRRILEDGVPADAVNETLISSYLYTSSLPDPDLIVRTAGEHRFSNFLLWQSAYAEY
                     YVTNVLWPDFDTDEVDCALSAYAQRIRRFGRLHPSELNDAGASAPTGPST"
     gene            complement(965..1522)
                     /gene="frr"
                     /locus_tag="VNL92_07300"
     CDS             complement(965..1522)
                     /gene="frr"
                     /locus_tag="VNL92_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWO94554.1"
                     /translation="MVTDPLGDARDRMAKALEATRREFDTIRTGRANPKLVEGLKVDY
                     HGHPTPLQQLANVTVPEAHLIAVQPYDRSSVAAIEKSIRASDLGLNPTNDGSTIRIAI
                     PPLTEQRRKELVKLVHKKAEEGRVAIRNIRRDAQDALRKRERAHEISEDEARRLLEQL
                     QKATDDFIHKVEEESKTKEAEVLEV"
     gene            complement(1541..2281)
                     /gene="pyrH"
                     /locus_tag="VNL92_07305"
     CDS             complement(1541..2281)
                     /gene="pyrH"
                     /locus_tag="VNL92_07305"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWO94555.1"
                     /translation="MFKRILLKLSGEALMGPGEFGLDPATLHSIAEQIRDVQAQGMEL
                     SIVVGGGNIFRGGAAARDIGMERATADYAGMLATLINALALQDALERAGLHVRTMSAL
                     SVTQVAEPYIRRRAVRHLEKGRVVIFAAGTGNPYMTTDTAAALRAIEIDAEVLLMAKN
                     NVDGVYDADPRKVPGAQRFDELTYIEALNRRLEVMDSTALSLCMENQLPIIVFALTAE
                     RSIVRAARGEPMGTWVGDTARKSAVSVQ"
     gene            complement(2329..2841)
                     /gene="tsf"
                     /locus_tag="VNL92_07310"
     CDS             complement(2329..2841)
                     /gene="tsf"
                     /locus_tag="VNL92_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWO94556.1"
                     /translation="MAVSAEVIRALREQTGAGIMDCKRALEDAQGDQERAAQLLRERG
                     IAIAEKKASRATSQGLVDAYIHAGGRIGALVELNCETDFVARTEDFRQLAHDLAMQVA
                     ATSPLAVGESDLPAGVEGDPKDLALLSQPFIKDPGRSVGDLVRDVIAKTGENIVVRRF
                     ARFELGGDQA"
BASE COUNT          454 a          920 c         1003 g          601 t
ORIGIN      
        1 ctctccggcg gcgaccgcga gagcgatggc gacgaccgcc acgtagactg gcccgccgac
       61 gatgacgagg acgacgagca gcggcaggcc gacgaacgcg gtggcgagcc gctgcttcag
      121 cacggaaggt tacgtcgagg ggccggttgg ggcgctcgcg ccggcgtcgt tgagctcaga
      181 cgggtgcaat cgcccgaagc gccggatgcg ttgcgcgtag gcgctgaggg cgcaatccac
      241 ctcgtcagta tcgaagtcgg gccagagaac gttcgtgacg tagtactccg cgtaggcgct
      301 ctgccagagg aggaagttcg agaatcggtg ctcgccagcc gtgcgaacga tgaggtcggg
      361 atcggggagg gaggacgtgt agagatagga tgagatgagg gtctcgttca ccgcatcggc
      421 cggcacgcca tcctcgagga tgcggcggac cgcatcgacg atctcgcgac gcccgccgta
      481 gttgaaggcg atggcaacgg tcatgcgatc gttcttgctc gtcagatcca ttgcctcgcg
      541 cacgcgacgc tgcagatcgt ccgagagatc gtcgagccgc ccgatgtgga cgacgcgtat
      601 gccgagccgg ttcagctcgg gaacctctcg ctcgatgacc gtatcgagca gatgcatcag
      661 ggcgtccact tcggactgtg gacgccccca gttctctgtg gagaaggcgt acagggtcag
      721 gtatcggacc cctcgattcg cgaacgcctc gatgacctgg cggatgttct ccgtcccggc
      781 gctgtgcccc tcctcgcgcg gcaagccacg ctgactggcc cagcggccgt tcccgtccat
      841 aatgacggca acgtgttccg gcaacgcggg atcgaggttt tcggcctgcg ctttgtctcg
      901 acgctcggcg actatggtgc ttgtcttctg tcgcatcggc tttccgagtg tagcagtcgg
      961 atatctagac ttccagcact tcggcttcct tggtcttgct ttcctcttcc accttgtgga
     1021 tgaagtcgtc ggtggccttc tgcaattgtt ccagcaggcg gcgcgcttcg tcctccgaga
     1081 tctcgtgggc tcgctcgcgc ttgcgcagcg cgtcctgggc atcgcgccgg atgttgcgga
     1141 tggcgacgcg cccctcctcc gccttcttat ggacgagctt gacgagctct ttccgtcgct
     1201 gctccgtgag cggagggatg gcgatgcgga tggtcgagcc gtcattggtg gggttgaggc
     1261 ccaggtccga tgcccgtatc gacttctcga tcgcggcaac gctcgagcga tcgtacggct
     1321 ggaccgcgat caggtgggct tctggaaccg tgacgttcgc caattgctgg aggggcgtcg
     1381 ggtggccatg gtagtcgacc ttgagcccct cgaccagttt tggattggcc cgcccggttc
     1441 ggatcgtgtc gaactcgcgg cgtgtcgcct cgagggcttt tgccatgcga tcacgggcat
     1501 cgccgagcgg atcggtgacc atggtcatgc ctccgcttcg ctactgtacg cttactgcgg
     1561 attttcgagc cgtatcgcca acccaagttc ccatcggctc gccgcgtgcg gcacgaacga
     1621 tgcttcgctc cgcagtgagc gcgaacacga tgatgggaag ttggttctcc atgcacagcg
     1681 agagagcggt gctatccatc acctccaggc gccggttcag ggcctcgatg taggtcagct
     1741 cgtcgaatcg ttgggcaccg gggactttgc gcgggtcggc atcgtaaaca ccatcgacgt
     1801 tgttcttcgc catcagcagg acttcggcat cgatctcgat ggcgcggagt gcggctgcgg
     1861 tgtcggtggt catgtacggg ttgcccgtgc ccgctgcgaa gatgacgacg cgtccctttt
     1921 cgagatggcg aaccgcgcgc cgccgtatgt agggctcggc gacttgcgtg acgctgaggg
     1981 ccgacatcgt ccgaacgtgg aggccggcgc gttcgagggc atcctggagg gcgagggcgt
     2041 tgatcagcgt cgcgagcatc ccggcatagt ccgccgttgc tcgctccata ccgatgtcgc
     2101 gcgccgccgc cccgccgcga aagatatttc caccaccgac gacgatgctg agctccatac
     2161 cctgcgcctg cacgtcgcgg atctgctcgg cgatggaatg tagggttgcg ggatcgaggc
     2221 cgaactcgcc cgggcccatg agggcctcgc cgctgagttt gaggaggatg cgcttgaaca
     2281 tcggcaggcc cctcgtggtg ttacgccgag ctgttgccgc gggtgcgact atgcttgatc
     2341 gccgccgagc tcgaaccggg cgaagcggcg gacgacgatg ttctcgcccg tcttcgctat
     2401 gacgtcccga acaagatcgc cgaccgatcg cccggggtcc ttgatgaatg gttgcgagag
     2461 cagggcaagg tccttcgggt cgccctcgac gcccgctggc aggtcgctct cgccgacggc
     2521 gagcgggctc gtcgcggcta cctgcatggc gaggtcgtgc gcgagttgcc ggaagtcttc
     2581 ggttcgcgcg acgaagtccg tttcgcagtt tagctcgacg agcgcaccga tgcggccgcc
     2641 cgcatggatg taggcgtcga cgagtccttg gctggtggcg cgggaggcct tcttctctgc
     2701 gatggcaatg ccgcgctcgc ggagcaattg tgccgctcgt tcctggtcgc cctgcgcatc
     2761 ttccagggca cgtttgcaat ccatgattcc cgcgccggtc tgctcgcgga gcgcgcggat
     2821 gacttccgcc gatactgcca cgtctttcct ccgtcgtctg gaagcggagc gctcgttgta
     2881 cgagctggct cgtcgctgca acctgtttcc cggactggag agcgccggga ttcatccgat
     2941 agcgagaagg ttctaccctt cgcttgctgc ggcgggga
//
LOCUS       DATMBB010000337         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_373057, whole genome shotgun sequence.
ACCESSION   DATMBB010000337 DATMBB010000000
VERSION     DATMBB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373057"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..>2459
                     /locus_tag="VNL92_07315"
     CDS             254..>2459
                     /locus_tag="VNL92_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWO94557.1"
                     /translation="MTTRIQRPLISPVLIERDRELEAIAECLERAETGLHTVTIAGEA
                     GLGKTRLGREAAALATQRGWLALEAGCAESDRVLELAPVRDLLHQLVAASSPEDVGRY
                     LGSEANELVALFPELADHEEPAPTVAVPAEGRQHLFRALVRFLIRVSRSHPLLLLMED
                     IHWSDESTLELLLCLARMARSERILVLATYRSDSVGEGLAHFLAELDRERVATELSLG
                     RLTRSGVASMVKAVLSWDRPAPTALLDRLYTLTEGNPFFIEEVLKVLTMQGEVASEER
                     VRELSVEVPTIPRTVQATIEARIALLSPGARRVLLWAATIGRRFDFAFLSVLEGIQEE
                     QLLAVLRELVAAQLIVEVSADRFALRHALIGEAVQARMLRRERRHLHLRVADAMERFY
                     AEDLDQHLADVAEHYYRAQAWSKVLEYAQRLGSRAQALHAPRAAIDCFTRALEAARHL
                     RASEPLALYRARGRAYEAAGEFDAARQDLERALNVSRMTDDQAGAWQSLIDLGLLWCA
                     RDYDRAGDYFRSALDLARELGDPARLGHSLNRLGNWHMNREEMGEALRRHEEALHIFR
                     NCGDQRGTAETLDLLGMTACISGDSSRGRSYYEEAISLFREMDDRLGLASALATMSGF
                     GGTYETDTVVPRMRSAEAVRAGEEAVRVAGEINWRSGEAYAQLELAFCLGSVGEYARA
                     FELGHAALALSDEIGHQQWLSGAHAALGALYLDVLSAPAAREHLEQALAVATE"
BASE COUNT          398 a          776 c          833 g          452 t
ORIGIN      
        1 gcacggtgat cgccgtctcg gtggcaggct tcgcgtcgtt ctcgtcgccg ggtagcgtgg
       61 atggtgtcgc tgccgtatca agcatggcgt ggctccagca tggcggaggc agcgcgttca
      121 ccggttaagg gagcgttaac aatgtcagca ggcggtcatc gcgggccccg atgggcctgc
      181 acgaggggcc cgcgttcgcg gcacacctga gcggctggac tccgagccca agcgtcgtgt
      241 atcttcatcg gccatgacga cgcgcatcca acgtccgctc atcagccccg ttctgatcga
      301 acgcgaccgg gagctcgagg cgatcgcgga gtgcctcgag cgagccgaga cgggtctcca
      361 taccgtcacg atcgctggtg aagccgggct tggtaagacg aggcttggtc gtgaagccgc
      421 ggcgctcgcc acgcagcgcg gctggctggc gctggaggcg ggttgcgccg agtccgaccg
      481 cgtccttgag ctggcccccg ttcgcgacct gctgcaccag ctcgtcgccg ccagttcgcc
      541 agaagacgtt gggcggtacc tgggtagcga agcgaacgag ttggtcgccc tgttcccgga
      601 actggccgac catgaggaac ctgcccccac cgtcgctgtg ccggccgagg gaaggcagca
      661 cttgttccgg gctctggttc ggtttctcat ccgcgtgtcg cgatcgcatc cgctcctcct
      721 gctcatggaa gatatccact ggagcgacga gagcacgctc gagttgttgc tctgcctcgc
      781 tcgcatggcc cgctccgagc gtatcctcgt cctggccacc taccgatcgg actcggtcgg
      841 cgagggacta gcccatttcc tggctgaact cgatcgcgag cgggttgcga ccgagttgag
      901 cttgggacgt ctcacgcgat cgggcgtcgc ctcgatggtg aaggcggtcc tctcctggga
      961 tcgccccgcc cctaccgcac ttctggatcg tctttacacg ctcacggaag gaaacccgtt
     1021 cttcatcgag gaggtgctca aggtcctgac gatgcaaggc gaggtcgcgt ccgaggagcg
     1081 tgtgcgggag ctgagtgtgg aggtcccgac catacctcgt accgtgcagg ccacgattga
     1141 ggcgcgtatc gcgttgctca gccccggcgc tcgccgggtc ctcctctggg cggccacgat
     1201 cggccggcgc ttcgacttcg ccttcctctc tgttctcgag ggcatccagg aagaacaatt
     1261 gctggccgtc cttcgcgagc tcgtggcggc acagctgatc gtggaggtgt cagctgacag
     1321 gttcgccctc cggcatgcgc tgattggcga ggccgtgcag gcgcgcatgc tccggcgcga
     1381 gcgccgtcac ctccacctcc gggtagctga cgcgatggag cgcttctacg ctgaggattt
     1441 ggaccagcac ttggcagatg ttgctgagca ttactaccga gcccaggctt ggagcaaggt
     1501 cctggaatac gcacaaaggc ttgggagcag ggcacaagcc cttcacgcgc cccgggccgc
     1561 catcgattgc ttcacgcgcg ccctggaggc cgcccgtcac cttcgagcgt ccgaaccgtt
     1621 agcgctctat cgagcgcgag gacgagctta tgaggcagca ggcgagttcg acgctgcccg
     1681 gcaggacctg gagcgtgcgc taaacgtgag ccgcatgact gacgaccaag cgggtgcgtg
     1741 gcagagcctg atcgacctcg ggttgctgtg gtgcgcccgc gattacgacc gcgccggcga
     1801 ttacttccgc tccgctctgg acctggcgcg ggaactgggc gaccccgcaa ggctaggcca
     1861 tagcttgaac cgccttggca actggcacat gaatcgagag gagatgggag aggcgctccg
     1921 tcggcatgaa gaggcgttgc acatcttccg caattgcggt gatcagcggg gcacagccga
     1981 aacgcttgac ctactgggca tgacggcctg catcagtggg gactcgtcga ggggaaggtc
     2041 ctactacgag gaagccatct cccttttccg cgagatggat gaccgcctcg gccttgcgtc
     2101 agcgctggcg acgatgtcgg ggttcggagg cacatacgag acagataccg tcgttcctcg
     2161 catgagatcg gccgaagctg tacgcgccgg agaggaggcc gtgcgagtgg caggcgagat
     2221 caactggcgg tctggcgaag cgtacgctca gctcgagctc gccttttgcc tgggctctgt
     2281 gggcgagtac gcccgagcgt tcgagcttgg gcacgcggcc ttggcgctca gcgacgagat
     2341 cggccatcag caatggctga gcggcgccca cgccgctctg ggcgcgttgt atctcgatgt
     2401 actgtccgct cccgcggcgc gagagcatct ggagcaggcc ctcgcggtgg ctacggaag
//
LOCUS       DATMBB010000338         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_145008, whole genome shotgun sequence.
ACCESSION   DATMBB010000338 DATMBB010000000
VERSION     DATMBB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145008"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VNL92_07320"
     CDS             <1..816
                     /locus_tag="VNL92_07320"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWO94558.1"
                     /translation="EDLPNAVALDAVAMQIAFPVGLPLTGVLIDAIGYGWTYVVTLWM
                     YISIIGALALMRYRRVLPPRQRSVLKDVRLGIAYTRQHTTVLWILLLTFCVNGLGFPV
                     VANLGPVWTSRILGLSATGVGLLAMTWGIGSMAASLAMTNVGHFPNKGWLLIAAAAGF
                     SACVVGFGYSRLIPLSAVLNLGLGGLLTVQSVSATSLVQRVVPNEVQGRVMSLFMMSQ
                     GVSQLLTLPIGAFAQWATFELTMPLVGWTSLALVLLIAATRPEVRNAGRSPEA"
     gene            complement(823..2937)
                     /gene="uvrB"
                     /locus_tag="VNL92_07325"
     CDS             complement(823..2937)
                     /gene="uvrB"
                     /locus_tag="VNL92_07325"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWO94559.1"
                     /translation="MAEFKLVSDFRPTGDQPQAIDALVEGVLKGYRRQTLLGVTGSGK
                     TFTMANVIARVNRPTLVLAPNRTLAAQLCSEFKEFFPENAVEYFVSYYDYYQPEAYIP
                     RTDTYIAKDADINDEIDKLRHAATRALFERRDVLIVASVSCIYGLGEPEEYQSFVLRF
                     ERGKPFSRARAMRRLVDMQYERNDMTLTRGRFRLRGDSITVQPSYEEVAVRIDFFGDE
                     VERIIEIDPLTGEVLGERDWVDIYPAKHFVTSREKLDQAIADIEVELRERVEELRAQG
                     KLLEAARLEERTQYDIEALREAGYCSGIENYSCHLARRHRPANGRMAVTCGSTPWTLL
                     DYFPDDFLMFIDESHIAVPQVRGMYHGDVSRKQTLVDYGFRLPSAIDNRPLSFEEFER
                     RLNQVIFVSATPGPYELEVSEQVVEQVIRPTGLLDPTIEVKPTRNQIDDLLHEIRVRV
                     ERGERALVTTLTKKMAEDLADYLQEMGIRTHYLHSEIDTLERVEILRDLRQGVYDVVV
                     GINLLREGLDLPEVSLVAILDADKEGYLRSGGSLIQTIGRAARHVNGHVIMYADTITD
                     SMRYAIDETNRRRAIQEAYNREHGITPEGIHKAIRDITDHVKKAAEQPVEYLGPVDLP
                     KDQMFRLIRDLETEMKAAARAMEFERAALLRDQIFELRRQLAATDEGDLLHEFARTSY
                     GSLRGRREGHRPRRISTSRRYR"
BASE COUNT          450 a          983 c          954 g          554 t
ORIGIN      
        1 gaagacctgc cgaacgccgt cgccctcgac gccgtcgcga tgcagatcgc attccccgtc
       61 ggcctgccgc ttacgggcgt gctcatcgac gccatcggct acggctggac gtacgtcgtg
      121 accctgtgga tgtacatctc gatcatcggg gcgctggcgc tgatgcgata ccgccgcgtt
      181 ctgccgcctc gccagcgctc cgtgctcaaa gacgtgcggc tcggcatcgc ctacacccgc
      241 cagcatacga cggtcctgtg gattctcctg ctcacattct gcgtgaacgg cctcggcttt
      301 ccggtggtcg cgaacctcgg gcctgtatgg accagccgga tcctcgggct gtccgccacc
      361 ggagtgggct tactcgcgat gacctggggc atcgggtcga tggcggcgtc gctggcgatg
      421 accaacgtgg gccactttcc gaacaaagga tggctgctga tcgccgccgc ggccgggttc
      481 tcagcgtgcg tggtcggctt cggctattcg aggctcatcc cgctgtccgc ggtgctgaac
      541 ctgggcctcg gagggctgct caccgtgcaa tcggtgtcag ccacatcgct cgtgcagcgc
      601 gtggtcccca acgaggtgca ggggcgagtg atgagcctgt tcatgatgag tcagggcgtc
      661 tcgcagctcc tcacattgcc catcggcgcc ttcgcgcagt gggcgacgtt cgagctgaca
      721 atgccgctgg ttggatggac cagcctggcg ctcgtgctcc tgatcgccgc gacccgcccg
      781 gaggtccgta acgcgggacg ctcgcccgag gcgtgaccac gcctacctgt atcgcctgct
      841 cgtactgatg cgccgcggcc ggtggccctc gcggcgtccc cgcagcgagc cgtaggacgt
      901 ccgggcgaac tcgtgaagca ggtcaccctc gtccgtggcc gcgagctgcc gccgtagctc
      961 gaagatctgg tcgcgcagaa gcgcggcccg ctcgaactcc atcgccctgg ccgccgcttt
     1021 catttccgtc tcgaggtccc ggatcaggcg gaacatctgg tccttcggca ggtccacggg
     1081 acccaggtac tcgacgggct gctcggccgc cttcttcaca tggtccgtga tgtcgcggat
     1141 ggccttgtgg atgccctcgg gcgtgatgcc gtgctcccgg ttgtacgcct cctggatggc
     1201 gcggcgccgg ttcgtctcgt cgatggcgta acgcatcgaa tcggtgatgg tgtcggcgta
     1261 catgatcacg tggccgttga catggcgggc cgcccggccg atagtctgga tcagcgagcc
     1321 acccgagcgc agataccctt ccttgtcggc gtccagaatc gcgacgagcg acacttcggg
     1381 aaggtcgagt ccctcgcgca acaggttgat gcccaccacc acgtcgtaca cgccctgtcg
     1441 caggtcacgc aggatttcga cccgctcgag cgtgtcgatc tcgctgtgca ggtagtgggt
     1501 gcggatcccc atctcctgga ggtaatccgc gaggtcttcg gccatcttct tcgtgagggt
     1561 ggtgacgagg gcccgttccc cgcgctcaac ccgcacccga atctcgtgca ggaggtcgtc
     1621 gatctggttc cgggtgggct tcacctcgat ggtgggatcg agcagcccgg tcggacggat
     1681 cacttgctcg acgacctgct cgctcacctc gagctcatag ggtccagggg tggccgacac
     1741 gaagatcacc tggttcagcc gacgctcgaa ctcctcgaag ctgagcggcc tgttgtcgat
     1801 ggccgacggc agccggaagc cgtaatccac gagcgtctgc ttccgcgaca cgtcgccgtg
     1861 gtacatgccc cgcacctggg gaacagcgat gtgcgactcg tcgatgaaca tcaggaaatc
     1921 gtccgggaag tagtccagga gcgtccacgg cgtggagccg caggtcacgg ccatcctgcc
     1981 gttggccggg cggtgccgcc gggccaggtg gcacgagtag ttctcgatgc ccgagcaata
     2041 gccggcctcg cgaagcgctt cgatgtcgta ctgcgtccgc tcttcgagcc gcgcggcctc
     2101 cagaagcttc ccctgggccc gcagctcctc gacccgctcc cgcagctcga cctcgatgtc
     2161 cgcgatggcc tggtcgagct tctcgcgcga cgtcacgaag tgcttcgcgg ggtagatgtc
     2221 cacccagtcg cgctcgccga gcacttcgcc cgtaagcggg tcgatctcga tgatgcgctc
     2281 tacctcgtcc ccgaagaagt cgatgcgaac ggccacctct tcgtaggaag gctgaaccgt
     2341 gatgctgtct cctcgcaggc ggaagcgacc acgggtgagc gtcatgtcgt tgcgctcgta
     2401 ctgcatatcg acgaggcgcc gcatcgcccg ggcgcggctg aacggcttgc cgcgctcgaa
     2461 ccgcagcacg aacgactggt actcttccgg ctcgcccaga ccgtagatgc aggacaccga
     2521 cgccacaatg agcacgtcgc gccgctcgaa cagggcgcgc gtcgcagcgt ggcgcagctt
     2581 atcgatctca tcgttgatgt ccgcgtcctt cgcgatgtag gtgtcggtcc tcgggatgta
     2641 cgcctcgggc tggtagtagt cgtagtacga gacgaagtat tcgaccgcgt tctcggggaa
     2701 gaactccttg aactcggagc agagctgcgc cgccagcgtg cggttcggcg cgaggaccag
     2761 cgtaggacgg ttcacccggg cgatcacgtt cgccatcgtg aacgtcttcc ccgatcctgt
     2821 cacgcccagc agcgtctgac gccggtagcc cttcaggacg ccctcgacga gggcgtcgat
     2881 ggcctgcggc tggtcgcccg tgggccggaa gtccgagacg agcttgaatt cagccatggc
     2941 a
//
LOCUS       DATMBB010000339         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_254385, whole genome shotgun sequence.
ACCESSION   DATMBB010000339 DATMBB010000000
VERSION     DATMBB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254385"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VNL92_07330"
     CDS             complement(<1..291)
                     /locus_tag="VNL92_07330"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: protein motif:HMM:NF014612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HWO94560.1"
                     /translation="MNVPGSGPLLRSLGLPPEAIERLSVERLQADAGILPWRGDELRL
                     ALRLGYAVGDADILKQLVISPGAIEAGRTALSSGRPVVSDVRMVASGIDRPPA"
     gene            complement(288..494)
                     /locus_tag="VNL92_07335"
     CDS             complement(288..494)
                     /locus_tag="VNL92_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94561.1"
                     /translation="MVMVCDVIRDRMREGLSAEFGQAVRFTHVDYQGTRVEAAPDVDA
                     AQFRASVQRIIDVQAALDAAGSAG"
     gene            complement(532..2511)
                     /locus_tag="VNL92_07340"
     CDS             complement(532..2511)
                     /locus_tag="VNL92_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit D family protein"
                     /protein_id="HWO94562.1"
                     /translation="MSRPIFPFTAVVGQEKMRLALALNAVNPKLGGVLIRGEKGTAKS
                     TAARALAALLPEIDAVAGCRFACDPHDPAAFCDECLARTELATVRRNVPFVELPIGAT
                     DDRVTGTLDMEHAIAEGRRRFEPGLLAAANRGVLYVDEVNLLNDHLVDVLLDAAAMGR
                     NYVEREGISFSHPAQFILVGTMNPEEGDLRPQLLDRFGLAVDVEGNGSTEERVEVLRR
                     RLAFEADPWGFVAQWSDAEQAERLRIEQARDLLQRMAPDDELLSLVARICAAYGVDGM
                     RADIAMVKTALTVAAYRGRDRVIDEDVRIASELALLHRRRRQPFDQSSFDSATVERLM
                     EELDSPPSPRSDETSRQAESPSADSGPDSGDAQQDRTFAIGTTFDPRRIEPQDGRRMP
                     RAALGRRERVDSPTLGTHVRSVPVAALPEGSLALAATIRAAAPHQRARRNGRAPSRRP
                     AILVEPCDLHVKLRESRAGNLIVFLVDASGSMAADERMGAVKGAIASLLLDAYRKRDR
                     VALVSFGGDGAKVGLPPTNSVELAQRLLADLPVGGRTPLAEGLHVATKLIERAGVLAP
                     NAEIRPLLVVLSDGRANVPFATDDALADAHRAAQALRDRVPSLVVDTETGHLRLGLAG
                     DLAAAMDAPCLRIEELDAAHLAQAVRVRLGSERGW"
     gene            complement(2508..>2912)
                     /locus_tag="VNL92_07345"
     CDS             complement(2508..>2912)
                     /locus_tag="VNL92_07345"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018499869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HWO94563.1"
                     /translation="RVFRTRVVNPKWIESVMRHGYRGGAEMAATVDYMFGYDATAGVV
                     EDWMYENVTRAYALDPAVQQFLRQHNPWALRGMIERLMEAAERGLWQEPSVEALQALR
                     DVYLEVDADLEGAGAGAVEGMQSEARPPGGPR"
BASE COUNT          475 a         1043 c          965 g          429 t
ORIGIN      
        1 ggcgggaggg cgatcgatgc cgctcgccac catgcgcaca tcgctgacga ccggccgccc
       61 ggaggacaac gcggtccggc ccgcttcgat ggcgccgggc gatatcacga gctgcttcag
      121 gatgtccgcg tcgcccacgg cgtagccgag tcgaagggcg aggcggagct cgtcgccgcg
      181 ccaggggagg atgccggcat cggcctgcaa cctctccacg ctgagccgct cgatcgcctc
      241 cggcgggagg ccgaggctac gcaggagtgg cccagacccc gggacgttca tcccgcgctc
      301 cccgcagcat cgagggccgc ctgcacatcg atgatccgtt gcaccgaagc gcggaactgg
      361 gccgcgtcca catccggggc ggcctcgaca cgcgtgccct ggtagtcgac atgcgtaaac
      421 cggaccgcct gcccgaactc cgccgacagg ccttcgcgca tccggtcacg gatgacgtcg
      481 cagaccatca ccattcgatc ctcatggaac gtgcacatgg ttcctcctct gctaccaacc
      541 ccgctcggaa ccgagccgca cgcggacggc ctgagccaag tgcgcagcgt cgagctcttc
      601 gatgcgcaga cagggcgcgt ccatggccgc cgcaaggtct ccagccaggc cgagcctcag
      661 gtggcccgtc tcggtgtcca ccacgagcga tgggacgcga tcgcgcagcg cctgcgccgc
      721 ccggtgcgca tcggccaagg cgtcgtcggt cgcgaaggga acattggccc tgccatccga
      781 aagaacgacg agcagcgggc ggatctccgc gttcggcgca aggaccccgg cccgctcgat
      841 gagcttcgtg gcgacatgca gaccctccgc gagcggcgta cgcccgccga ccgggaggtc
      901 ggcgagcaag cgttgcgcca gctcaacgct attggtcggc ggcaggccca ccttcgcgcc
      961 atcccccccg aacgagacga gcgccacgcg atcgcgcttg cggtacgcat cgagcagaag
     1021 ggacgcgatc gcgcccttga ccgcacccat gcgctcgtcg gctgccatcg agccgctggc
     1081 atccacgaga aagacgatga gattgcccgc gcggctctcg cgtagcttga cgtgcaggtc
     1141 gcaaggctcc actaggatcg caggccttcg cgacggggcc cggccgtttc gtcgcgctcg
     1201 ttgatgcggc gctgcggccc ggatggtcgc ggcgagggcc agcgatccct cgggaagcgc
     1261 ggctaccggt acggagcgga catgcgtccc gagcgtcggc gaatcgacgc gctcccgccg
     1321 gccaagcgca gcgcgtggca tgcggcgccc gtcctgcggc tcgatgcgcc gcgggtcgaa
     1381 tgtggtgccg atggcgaacg tgcggtcctg ctgcgcatcc ccggagtctg ggccggaatc
     1441 ggccgacggg gattcggctt gcctcgacgt ctcgtcgctc cgaggcgatg gaggcgaatc
     1501 cagctcctcc atgagccgct cgacggtggc cgaatcgaac gacgactgat cgaacggctg
     1561 ccggcgacga cgatggagca aggccagctc cgacgcaatg cgtacgtcct cgtcgatcac
     1621 ccggtcgcgg ccgcggtagg ctgccacggt aagggcggtc ttcaccatcg cgatgtcggc
     1681 gcgcatgcca tcgacgccat acgctgcaca aatgcgcgcc accaacgaca acagctcatc
     1741 gtccggcgcc atgcgttgga gcaaatcgcg cgcctgctcg atacgaaggc gctcagcctg
     1801 ctctgcatcg ctccactgtg cgacgaagcc ccacgggtcg gcctcgaagg caagccgccg
     1861 acgcaagacc tcgacacgct cctccgtgct gccgttgccc tcgacgtcga ccgccaggcc
     1921 gaatcgatcg agcagctggg gccggagatc gccctcctcc gggttcatcg tgccgacgag
     1981 gatgaactgc gccgggtggg agaacgagat cccctcgcgc tcgacgtagt tgcggcccat
     2041 cgccgcggca tcgaggagca catccacgag gtgatcgttc aggaggttca cctcgtcgac
     2101 gtagaggacg ccgcgattcg cggcagccag cagcccgggc tcgaatcggc ggcggccttc
     2161 ggcaatggcg tgctccatat cgagcgttcc cgtcacccgg tcgtccgtgg cgccgatcgg
     2221 cagctcgacg aacggcacgt tacgccgaac ggtcgcgagc tcggtccggg cgaggcactc
     2281 gtcgcaaaac gccgcgggat cgtgcggatc gcaggcgaag cggcacccgg cgaccgcgtc
     2341 gatctcggga agcagggctg ccagggcgcg cgctgcggtg gactttgcgg tccccttctc
     2401 gccgcgaatg aggacgccac cgagctttgg attcacggcg ttcagggcca gcgcgaggcg
     2461 catcttttcc tgccctacga cggcggtgaa ggggaagatc ggccggctca tcgtggcccc
     2521 cccggcggcc gagcctccga ttgcatgcct tcgacggccc ctgcccccgc tccctcgaga
     2581 tccgcgtcga cctccaggta gacgtcgcga agcgcctgca gcgcctcgac acttggctcc
     2641 tgccacaagc cccgctccgc cgcctccatg aggcgctcga tcatgccacg gagggcccac
     2701 gggttgtgct ggcggaggaa ctgctgtacg gccggatcga gggcgtaggc acgggtcacg
     2761 ttctcgtaca tccagtcctc cacaaccccg gccgtcgcgt cgtatccaaa catgtagtcg
     2821 acggtggccg ccatctcggc gccgcctcgg tagccatgcc gcattaccga ctcgatccac
     2881 ttcggattca cgacccgtgt ccggaacaca cg
//
LOCUS       DATMBB010000340         4031 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_55875, whole genome shotgun sequence.
ACCESSION   DATMBB010000340 DATMBB010000000
VERSION     DATMBB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55875"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..173
                     /locus_tag="VNL92_07350"
     CDS             27..173
                     /locus_tag="VNL92_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HWO94564.1"
                     /translation="MHPAQCADCGDMTEVPFRPRGDRPVYCSACYNKQRAGSTRTAPE
                     TPTF"
     gene            227..319
                     /locus_tag="VNL92_07355"
     tRNA            227..319
                     /locus_tag="VNL92_07355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Ser,seq:gct)
     gene            394..1692
                     /gene="miaB"
                     /locus_tag="VNL92_07360"
     CDS             394..1692
                     /gene="miaB"
                     /locus_tag="VNL92_07360"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWO94565.1"
                     /translation="MVKRYHIWTAGCQMNKADSSKLAAGLDKLGYVAVDEPAAADVVV
                     LNTCAVRQGAEDRAYSELGRLRKLKQERGDVRIAVMGCMIGPRSAELQRRFPFVDVFA
                     RPQAFDDVIFALGEDPDDLGGEFWPDAIGEPAGPTAFIPVIHGCDKFCTYCIVPYRRG
                     RERSRSIDDVQHEARALVARGVREVTLLGQTVEAYGHDLPDRPDLSDLMNVLAEIPGL
                     DRIRFLTSYPKDMTERIIASVASNPKVCEYFSIPVQSGDDDVLASMRRGYTVDEFRDR
                     VHKIRSVMPEAGITTDVIVGYPQETDAQFENTLRLLEELSFDKVHVAMYSPRPGTIAS
                     RKLPDDVPPDVKQQRLRAIEDLEREISSAINTRLVGGSQPVLVETITGDQATGRTRTG
                     KLVHFHAPGVAIGETVDVHIERATAWSLKGSVAAAAVGAT"
     gene            1698..2360
                     /locus_tag="VNL92_07365"
     CDS             1698..2360
                     /locus_tag="VNL92_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWO94566.1"
                     /translation="MVTSAGAATAATPAVAVILVILTVDDDRLKVLLVRRSAEPERGA
                     WALPGGLLPPHEAPENAAGRKLLEETGMRDVFLEQLYTFSDLDSTKPSIAIAYYALVH
                     RDVARLRDSEAWHPAWHDVDDLPELGLRNDRVIAYARERLRAKLEYTNIAYSLLPRHF
                     TLSRLQRVYEAIAGRPLDKRNFRRRMLSLGLVRGIGATTREGAHRPAQLYEFASQTPT
                     VL"
     gene            2591..3052
                     /locus_tag="VNL92_07370"
     CDS             2591..3052
                     /locus_tag="VNL92_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HWO94567.1"
                     /translation="MHVTIDGFGGDRVRLADDALVRELLDRYPAEIGMTKIAPPHVVR
                     YVGSKPEDWGVSGFVLIAESHISVHTFPERGYIWVDIFSCKNFDASLAIESVRKEFGL
                     ERWHTNVLDRGLLEYPHEVEAAAVVSQEERRRVTASVPPGKDQTIARAANR"
     gene            3098..>4031
                     /locus_tag="VNL92_07375"
     CDS             3098..>4031
                     /locus_tag="VNL92_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HWO94568.1"
                     /translation="MHDVKANGEHQLFEQKVEPFEVPTTPGAADLMRRLGGTSFQARA
                     LATATDIWHRALEDDVTIFFGLAGAMVPAGMRQVLVYLIEHRMVDCIVSTGANLFHDV
                     YETLGHPHWKHRPDTDDVALGRLRVNRFYDVLAPENTFFGAEKFVTSFALTLDPGRPY
                     TTREYLRLLGEAMIPLARTEGVLTAAAKHDVPIYSPALGDSVHGMAIAYARTHQNHPV
                     IFDVIGDVIETSSIAVQSKGTAVIYVGGGTPKNFIQQAEVASYIFDRELPGHRYGIQI
                     TMDQPQWGGLSGCTFEEAQSWRKIAPDATTVTVNV"
BASE COUNT          715 a         1352 c         1285 g          679 t
ORIGIN      
        1 gcttcggcgg ccgcactccc cgccagatgc acccggcgca gtgcgcggac tgcggcgata
       61 tgacggaggt gcctttccgt ccgcgcggcg accggcctgt ctactgcagc gcctgctaca
      121 acaagcagcg cgccggctct acgcggaccg caccagagac ccccaccttc taggccgagc
      181 tcacgtgagc ttgcggcctc gaattcggcg ttgctacaat gccggtggag ggatcgcata
      241 gaggtctagt gcggccgcct gctaagcggt tagggggatg ttctccccct catgggttcg
      301 aatcccattc cctccgccag ccatgagacg aatcgtatcc ggcccttctt cttcgaaggg
      361 ccggtttcgt cgtcctacct cgcaaggagc ggaatggtga agcgatacca catctggacg
      421 gccggctgcc agatgaacaa ggccgattcg tcgaagctcg ctgcgggcct cgataagctc
      481 ggctacgtcg cggtcgacga gccagccgcc gccgatgtcg tcgtcctcaa cacctgtgcc
      541 gtccgccagg gggcggagga ccgcgcctat agcgagctcg gtcgcttgcg caagctcaag
      601 caggagcgcg gcgacgtccg catcgcggtc atgggatgca tgatcggccc gcgctccgcc
      661 gagttgcagc gccgcttccc cttcgtcgac gtcttcgccc gcccacaggc cttcgacgac
      721 gtgatcttcg cgctcggcga agaccccgac gacctcggcg gcgagttttg gcccgacgcc
      781 atcggcgagc ccgccggtcc gaccgcgttc atacccgtga tccacggctg cgacaagttc
      841 tgcacgtact gcatcgtccc ctatcgccgt gggcgggaac gaagccgctc gatcgatgac
      901 gtccagcacg aagctcgcgc gctcgttgcc cgcggggtgc gagaagtcac gctcctcggg
      961 cagaccgtcg aggcgtacgg acatgacctg cccgatcgcc ccgacctctc cgacctcatg
     1021 aacgtgctcg cggagattcc gggcctcgac cggatccgct tcctcacctc gtatccgaag
     1081 gacatgaccg agcgcatcat cgcgagcgta gccagcaacc cgaaggtgtg cgagtacttc
     1141 agcatcccgg tgcaatcggg ggacgacgat gtcctggcat cgatgcgacg cggatacacc
     1201 gtcgatgagt tccgggaccg cgtgcacaag attcgcagcg tcatgccgga ggctggaatc
     1261 accacggacg tcatcgtcgg ctatccgcag gagacagacg cgcagttcga gaacaccctc
     1321 cgtctgctcg aggagctttc gttcgacaag gtacacgtag cgatgtactc gcccaggccg
     1381 ggcaccatcg cctctcgaaa gctgcccgac gacgtgcccc ccgacgtgaa gcagcagcgg
     1441 ctccgtgcca tagaagacct ggagcgcgaa atctcatccg caatcaacac gcggctcgtc
     1501 ggcggctccc aacccgtgct agtcgagacg ataaccggcg accaggcgac ggggcgaacg
     1561 cggacgggca agctcgtgca cttccacgcg cccggcgtcg cgattggcga gacggtcgat
     1621 gtgcacattg agcgcgccac cgcgtggtcg ctcaagggga gcgttgcggc ggcggccgtg
     1681 ggagcgacat agagcggatg gtcacgtcag cgggcgcggc gacggccgcc actccggcgg
     1741 ttgccgtcat cctcgtgatc ctcacggtgg atgacgaccg actcaaggtg ctgctcgtcc
     1801 gccgcagcgc cgagcccgag cgcggtgcat gggccttgcc cgggggtctg ctgcccccgc
     1861 acgaggcgcc ggagaacgca gcggggcgca agctgctcga ggagaccggc atgcgggatg
     1921 tcttcctcga gcagctgtat acctttagtg atctcgattc gacgaagccg tccatcgcca
     1981 tcgcctacta cgccctcgtc catcgcgatg tcgcacgtct tcgcgatagc gaggcatggc
     2041 atcccgcgtg gcacgacgtc gacgacctgc cggagctcgg cttacgcaac gaccgcgtca
     2101 tcgcgtatgc gcgggagcgg ctgcgggcga agctggagta cacgaacatc gcctacagcc
     2161 ttctgccgcg tcacttcacg ttgagccgcc tgcagcgggt atacgaggcg atcgcagggc
     2221 gtccgctgga caagcgcaac tttcgccggc gaatgctgtc tctcggcttg gtccgaggca
     2281 tcggggcaac gactcgggaa ggcgcacacc ggcccgcgca actctacgag ttcgcctcgc
     2341 agacgccgac cgtgctttga tcggcgagcc ggcccgactc ggaaggcttg cggccacgaa
     2401 aagggctatg gtagcatcgt ggatgtgctc gcgttcagcg agccgctgag tcgtcttact
     2461 cggatcggcg gcgacgagcc gcactaaccc actggccgtt cggcggtgtc gacgggaagt
     2521 acgccgccgg ggggcctttt ccctcgctca acaccaacac tgcacatcta cccagaaagg
     2581 ggggcgccgg atgcacgtga cgatcgatgg cttcggcggg gaccgcgttc gcctcgcgga
     2641 cgatgcgctc gtgcgcgagc tgctcgaccg atacccggcg gagatcggca tgaccaagat
     2701 cgctccgcct catgtcgtac ggtatgtcgg cagcaaaccg gaggattggg gcgtgtccgg
     2761 gttcgtgttg atcgccgaga gccatatcag cgtgcatacg ttccccgaaa ggggctatat
     2821 ctgggtcgac atcttttcct gtaagaactt cgatgcgtcg ctggcaatcg agagtgtccg
     2881 caaggagttc gggttggaac gctggcacac caacgtgctc gaccgcggct tgctcgagta
     2941 tccgcacgag gtcgaggctg ccgcggtcgt ctcgcaagag gagcgtcgtc gcgtgacggc
     3001 gagcgtccct ccagggaagg accaaacgat cgcccgcgcc gccaaccgct gatcggaggg
     3061 ggtgcctccc tccggcggcc cactgggagc tcgcatcgtg catgacgtga aagcgaacgg
     3121 cgaacaccag ttgttcgagc agaaggtgga gcccttcgaa gtccccacga ctcccggcgc
     3181 cgcggacctc atgcggcggc tcggaggcac cagcttccag gcccgggcgc tcgcaaccgc
     3241 aaccgacatt tggcatcgcg ccctggagga cgacgtcacg atcttcttcg gcctcgcggg
     3301 ggccatggtt cccgccggca tgcggcaggt cctcgtctat ctcatcgagc atcgcatggt
     3361 cgattgcatc gtatcgaccg gagcgaacct gttccacgac gtgtacgaga ccctcggcca
     3421 tccccattgg aagcataggc cggatacgga cgacgtggcg ctcgggcgcc tacgtgtcaa
     3481 ccgcttctac gatgtgctcg cgccggagaa cacgttcttc ggcgcagaga agttcgtcac
     3541 cagcttcgct ctcacgctgg atcccggccg cccctacacc acgcgcgagt acctccgcct
     3601 cctcggcgag gccatgattc cactggcgcg gacggaaggc gtgctcacgg cagcggccaa
     3661 gcacgatgtc cccatctaca gtcccgccct aggcgacagc gtccatggga tggcaatcgc
     3721 ctacgcacgg acgcaccaga atcaccccgt catcttcgac gtcatcggcg acgtcatcga
     3781 gacgtcttcc atcgccgtgc agtcgaaggg gacggccgtc atctacgtcg gtggcgggac
     3841 gcccaagaac ttcattcaac aagcggaggt ggcgagctac atcttcgatc gcgagcttcc
     3901 gggccacagg tacggcatcc agattacgat ggaccagccg cagtggggcg gcctttcggg
     3961 ctgcacgttc gaagaggccc agagttggcg caagattgct cccgatgcca cgacggtgac
     4021 cgtgaacgtc g
//
LOCUS       DATMBB010000341         3247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_214176, whole genome shotgun sequence.
ACCESSION   DATMBB010000341 DATMBB010000000
VERSION     DATMBB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214176"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VNL92_07380"
     CDS             complement(<1..345)
                     /locus_tag="VNL92_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWO94569.1"
                     /translation="MKGDRSAQLRILTIPLDYNKVYALVGEDGLVLIDSGPDFVGAWD
                     EIVGALRRAGFEAKHVQSVLLTHAHPDHSSLAARWQHDVGAEIVAPRGEEPALLLGPE
                     FVPSQREVVRGFL"
     gene            complement(342..1580)
                     /locus_tag="VNL92_07385"
     CDS             complement(342..1580)
                     /locus_tag="VNL92_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HWO94570.1"
                     /translation="MKAEIVSVGTEILLGEILDTNAQYIAARLPALGIDCNRMSQIGD
                     NLQRLTEAFRSAWEQSDLVIITGGLGPTEDDLTREALAAALDEPLSIDPGLEEQIRSL
                     FANRGWPMPERNIKQAMLIPSARPIPNPRGTAPGWWVEREGHLAVAMPGVPPEMYHMW
                     ENEVAPELRRRETGAVIISRTVKTAGIGEGTVDEMVSHLLKSSNPSIGVYAKIDGIHL
                     RITAKAATEDECRDMIRPLEDEVLRILGPSVWGFDDDTLETSIGRTLTERCLTLATME
                     SCTGGLLASTLTEGDGASSYFLGGVVAYATEQKIAHGVDASLVEQHGVISSQVAVDMA
                     RAVRERVGSDIGLGITGVAGPALQEDKPVGTIHIGLDYRGAQESVSYTFPQGRQQVRA
                     RAVTTALFLLRRVLSAEAAG"
     gene            complement(1739..2917)
                     /locus_tag="VNL92_07390"
     CDS             complement(1739..2917)
                     /locus_tag="VNL92_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWO94571.1"
                     /translation="MPQAVVDRIKVIDADTHVSEPPDLWTSRVSNKWGELVPHVVFDD
                     ERKEEVWYFGGSRYHPTAGAAMAGWREFPPAHPPSLDEADRASWDSVARLQRMDEYGI
                     HAQVIYPNVGGFGSGRFLQLGEPELMLECVQAYNDFLIDWIQPNPERFVPIMALPFWD
                     VEAATREIQRCHKKGHKGILFGSEPHIFGQPHLADPHWNPIWETAQELGLTINFHIAS
                     GDMTQLFGSGYPGNGRQANYAKTTVMFFLDNARAVMEVIISGICHRYPRLNFVSVESG
                     VGWVPFVLEALDWQWLNSGCQQEHPEMDLLPSEYFKRQVYACFWFERGSAEKAIELVG
                     ADNLLYETDFPHPTSMSPGPASFADNPKDFIEQNLSKLPEDVLRKILHDNAARLYHLS
                     "
     gene            3103..>3247
                     /locus_tag="VNL92_07395"
     CDS             3103..>3247
                     /locus_tag="VNL92_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010540205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HWO94572.1"
                     /translation="MDRSKFRDSVAIVGIGSTEFSRNSGRSELQMAAEAILEAVDDAG
                     LRID"
BASE COUNT          549 a         1079 c         1078 g          541 t
ORIGIN      
        1 caggaacccg cgcaccacct cccgctgcga aggcacgaac tcggggccaa gcagcagtgc
       61 cggctcttcg cctcgcggcg cgacgatctc agcgccgacg tcgtgctgcc agcgggccgc
      121 gaggctgctg tggtcgggat gagcgtgagt gaggaggacg gactgcacgt gcttcgcttc
      181 gaagccggcc cgccgaagcg cgcccactat ctcgtcccaa gcgccaacga aatcggggcc
      241 agagtcgatc agcaccaggc cgtcctcgcc tacgagcgcg tataccttgt tgtagtcgag
      301 ggggatggtg aggatgcgga gctgggcgga gcggtcgccc ttcacccagc cgcctcggcg
      361 gagaggactc gcctcagcag aaacagcgcc gttgtgacgg cgcgggcgcg cacctgctgc
      421 cggccttgag ggaaggtgta gctcaccgac tcctgggcgc cgcgataatc gaggccgatg
      481 tgaatcgtcc ccaccggctt gtcttcctgg agggcggggc ctgccacgcc ggtaatcccc
      541 aagccgatgt cggaccccac acgctctcga accgcacgcg ccatgtccac cgccacctgg
      601 ctgctgatga cgccgtgctg ctcgacgagg ctagcatcga cgccgtgcgc gatcttctgc
      661 tccgtcgcgt aggccacgac ccccccgagg aagtagctcg aggccccgtc gccctccgtg
      721 agggtgctcg ccagcagacc gcccgtgcac gattccatgg tggcgagcgt aaggcaccgt
      781 tccgtcagcg tgcggccgat cgacgtctcg agggtgtcgt cgtcaaatcc ccacacggaa
      841 gggcccaaga tccgcagcac ctcgtcctcc aagggacgga tcatgtcgcg gcactcgtcc
      901 tcggtggctg ccttggcggt gatgcgaagg tggatgccat cgatcttcgc gtacacgcca
      961 atgctcggat tcgacgactt caggagatga ctcaccatct cgtcgacggt tccctctccg
     1021 ataccggccg tcttcacggt acgggagatg atgacggcgc ccgtttctcg ccgccgcagc
     1081 tcgggcgcca cctcgttctc ccacatgtga tacatctccg gggggacgcc gggcatcgcc
     1141 accgccaggt ggccctcgcg ctcgacccac cagcccggcg ccgtcccgcg cgggttgggg
     1201 ataggacgtg cggaggggat gagcatggcc tgcttaatgt tgcgctccgg catgggccag
     1261 ccgcggttcg cgaagagcga gcggatctgc tcctcgaggc ccggatcgat ggagagcggc
     1321 tcgtcgagcg ccgccgcgag cgcctcgcgc gtcagatcgt cctctgtcgg cccgaggccc
     1381 ccagtgatga tgacgaggtc gctctgctcc caggcggagc ggaaggcttc ggtgaggcgc
     1441 tgcaggttat cgccgatctg cgacatgcgg ttgcaatcga tgccgagggc ggggaggcgc
     1501 gcggcgatgt actgagcatt ggtatcgagg atctcgccca ggaggatctc cgtgccgacg
     1561 gagacgatct cggccttcac gcacgtccct cccccgtagt cgtcgcgagc cgagcatacg
     1621 agcggccctc gcgaggcgtc aagctcgcag cggacttgtc ggcgccgaga gcccccgcga
     1681 ggccggtggt gctggtgagg acgaagccga ggccggtcgc tgggaccggc ctctcgccct
     1741 agctcaggtg gtagaggcgc gcggcgttat cgtgcaggat cttgcgcagc acgtcctcgg
     1801 gcagcttgct caggttctgc tcgatgaagt ccttcggatt gtcggcgaag gaggcggggc
     1861 ccggcgacat gctcgttggg tgcgggaagt cggtctcgta caagaggttg tcggcgccga
     1921 ccagctcgat cgccttctcc gcgctgccgc gctcgaacca gaagcaggcg tacacctgcc
     1981 gcttgaagta ctcgctcggc agcaggtcca tctccggatg ctcctgctgg caaccgctgt
     2041 tcagccactg ccaatcgagc gcctccagca cgaagggcac ccagcccacg ccgctttcca
     2101 cggacacgaa gttgagccgc ggataccggt ggcagatgcc ggagatgatg acctccatga
     2161 cagcgcgagc gttatcgagg aagaacatca cggtcgtctt ggcgtagttc gcctgacgtc
     2221 cgttgcccgg atacccggag ccgaacagct gcgtcatgtc gcccgaggcg atgtggaagt
     2281 tgatggtaag gcccagctct tgcgccgtct cccaaatggg attccagtgc gggtccgcga
     2341 ggtgcggctg gccgaagatg tgcggctcgg agccgaagag gatgcccttg tgacccttct
     2401 tgtggcagcg ctgaatctcg cgcgtcgccg cctcgacatc ccagaacggg agggccatga
     2461 tgggcacgaa gcgctctggg ttcggctgaa tccagtcgat caggaagtcg ttatacgcct
     2521 ggacgcactc caacatgagc tcgggctcgc cgagctggag gaagcgcccc gagccgaacc
     2581 cgcccacgtt cggatagatg acctgagcat ggatgccata ctcgtccatg cgctggaggc
     2641 gcgcgacgga gtcccacgag gcgcgatccg cctcgtcgag gctcggcgga tgcgccggag
     2701 ggaactcccg ccatccggcc atcgccgcgc cggccgtggg gtgatagcgc gacccaccga
     2761 agtaccacac ctcttcctta cgctcgtcgt cgaagacgac gtgcggcacg agctcccccc
     2821 acttgttgga gactcgcgac gtccacaggt ccgggggctc cgagacgtgg gtatccgcat
     2881 cgatgacctt gatccgatcg accacagctt gcggcatgaa cttccccctc ctcatcgatt
     2941 cgtccggcga acagtggaac atgaagatag cgcgccgcga cgccgatggc aagctcttgg
     3001 caaacccgcc cattgccggg cggaactccc acggcataca atcggcgcgg acgtggcata
     3061 ggggaacctc gccctactcg cccatgcacg ggaggcagaa ctatggaccg cagcaagttc
     3121 cgggacagcg tggcgattgt cggcattggc tccactgagt tctcgcgcaa ctccggtcgc
     3181 agcgagctgc agatggccgc ggaggccatc ctcgaggcgg tcgacgatgc gggcctgcgc
     3241 atcgacg
//
LOCUS       DATMBB010000342         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_312310, whole genome shotgun sequence.
ACCESSION   DATMBB010000342 DATMBB010000000
VERSION     DATMBB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312310"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..806
                     /locus_tag="VNL92_07400"
     CDS             378..806
                     /locus_tag="VNL92_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94573.1"
                     /translation="MALREVERRFEIARALFELSHDQRIALASNELERFNELLEERKV
                     LLLELEQEGPLSSAPYNVIPFPGEVALGARRIAQTDDAIAVQSIIRAILHIDAENEAR
                     LAARLDDMRETLSTLADEQTSGGSRGDDARPALGVGKSQP"
     gene            1016..1279
                     /gene="flgM"
                     /locus_tag="VNL92_07405"
     CDS             1016..1279
                     /gene="flgM"
                     /locus_tag="VNL92_07405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWO94574.1"
                     /translation="MNDAETHSRETADDARERRARRSSQKRDVVAVSERARLLKQADA
                     AVRASKDVRTDRVERLKKKVRTNTYEVDAERIAERLLESDKNP"
     gene            1506..>3559
                     /locus_tag="VNL92_07410"
     CDS             1506..>3559
                     /locus_tag="VNL92_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWO94575.1"
                     /translation="MRVPGSGGEKMTLMAARTVSPDESWDNICSRTGATVVFALEAAD
                     DRLLFRNVAVAEERFRGLVYALTKRHLTDGETPRPSLPLGGATLLREVVVSERSADLD
                     AFVAGHPLDDVAQAYLRQLTLALPAPHVTLVQVIDNSWERRIALFLRATPTTETDLVQ
                     LERTVRMAEWPRVPDVISRLATSYERDLKDVAATLRSPALHDDLASVYQAVSLAVGAD
                     RVVVLEANRHSAQVRVKAQWPEHDGPVQPLRPGEVIQTQQAAFADMMNGSIRIFPQLT
                     DGRTIEDDEHVLAAGYRSALYLPLPDHTLGPCIVVALSRNPRQYDEATVSQLRMALLP
                     ATGVAEGPSPSRQPIDDRLQLAIDIGSGPIVVLDPESFQVRLLNNEADRYFGYGRNEM
                     LGRSIVELVEGGVDLIDSVRRGRSHVEPVSLRTAGGRKPARLDFIQHRTGDSEVLLLM
                     IRDLSKAYQRVEYFGALNAVADRAEAASGPAEAARAIVEALNELAGSYAIYEPADDGF
                     AVAATNLPPGVDLRELPGLFPGASREAVYAELPASLTKLVDPAAARELGVIVAPVKIE
                     DSVKAVVALVSSNLGELDRSAVQGFAARLALALRERATAAEAAALSVPAIDMPSEVAS
                     AVIDATDYQTLLTGALRALSHHAPFEVAVLRTCSAGRETIRIVSERPLGVDIIARLVE
                     IS"
BASE COUNT          630 a         1218 c         1148 g          563 t
ORIGIN      
        1 ccgcgctgct tcgatggcct tgccccgccg aaggtgagct acaagcagct ccgttccggc
       61 cgcggctcgg cgggcgccgc tccggctggg atactccagc gccgacgaca ggacgtcttg
      121 gcaccgattg gcagcgtagc gccccctatt tcgcgcgatg gggtggccgg attcggagtt
      181 gtgcactccg caagcgaggg tccgaacctt cctccacgcc tcgatgggtg cctacagggt
      241 catgcacgcg tgtcgataat cacaagagga acgtaggccg gacttccccc cggaccgatc
      301 gcgcaaggat gaaccatccc ggccgcatca accggctccg ttcatgtgca cggagcgcga
      361 acgaaagagg agaacgcatg gccctccgag aagttgagcg tcgattcgag attgcgcgtg
      421 cgctcttcga gctgagccac gatcagcgaa tcgcgctcgc ctctaatgag ctcgagcgct
      481 tcaacgagct tctcgaggag cgaaaggtgc tcctgctcga actcgagcag gaaggtcctc
      541 tctccagcgc tccttacaac gtgatcccct tcccggggga ggtggcgctc ggtgcccggc
      601 gcatcgccca aacggatgac gccatcgcgg tgcagagcat catccgagcc atcctgcaca
      661 tcgatgccga gaacgaagcc cggctcgcgg cgcggctcga tgacatgcgg gaaacgctct
      721 cgaccctggc cgacgaacag accagcgggg gcagccgggg ggatgacgcc cggcccgcgc
      781 tcggggtcgg caaatcgcag ccatgagcgg ttccggacca gaggtcgagc aggacacgcg
      841 ccgagaatcc atcgcgccgc acggcgtcgt tccctcgagc catcgccggc gcccgccgtt
      901 tgtgcaatct gaggagacgg gctacactgt cgcacagggg agccgcgagg ctcccggggg
      961 caaagggagt ccttcggtga agtcgaacgc gagccgaggc agccgaactg cgaagttgaa
     1021 tgacgcagag acgcacagcc gggagacggc agatgacgcg cgagagcgac gcgcccggcg
     1081 atcctcgcag aagcgagatg tggtggccgt gagcgagcga gcacgcctcc tgaagcaggc
     1141 cgacgcggcc gtccgcgcct cgaaggacgt tcgcaccgat cgcgttgagc gcctcaagaa
     1201 gaaggttcgg acgaacacct acgaagtcga cgccgagcga atagccgagc ggctgctcga
     1261 atccgacaag aacccttagc cgagctatcg taaccccaga ccagagcata cccaacctcg
     1321 cccctccacg ccgaatcgcg cccgccaaac agggggaacc ctgatagctc gcccatccca
     1381 tcaaagctaa gataagtgcg acgttccgga ctacgccggc atgatcgcgc gcagcttcct
     1441 tgacgcatac ctcgctgatt caggattgag ttgcgcgtgt cgatactagt atcgaggtcc
     1501 gcaacgtgcg cgtacccggg agcggcggtg agaagatgac gctgatggca gcgaggacgg
     1561 tatctccaga cgagtcgtgg gacaacatct gttcgcgtac cggcgcgaca gtggtgttcg
     1621 ccctcgaggc agcggacgat cggttgctgt tccgcaacgt tgccgtggcc gaggaacgat
     1681 tccgcgggct cgtatacgcg ctcacgaagc gccatctcac cgatggggag acgccccggc
     1741 caagcctgcc attgggtgga gcgactctcc ttcgcgaagt ggtcgtgtcg gagcgttccg
     1801 ccgacctcga tgcctttgtc gcgggccatc cgcttgacga cgtcgcgcaa gcttaccttc
     1861 gccagctaac cctggccctc cccgctcccc acgtaaccct cgtgcaggtt atcgacaaca
     1921 gttgggagcg acggatcgcc ctcttccttc gtgcaactcc tacgaccgag acggacttgg
     1981 tgcaactcga gcggaccgtc cgcatggccg agtggccccg cgtcccggac gtgatcagcc
     2041 ggctcgcgac gtcgtacgag cgcgacctca aggacgtcgc ggctacgctg cgttcgcctg
     2101 ccctccacga cgacctagcc tccgtatacc aagcggtgag cttggctgtc ggcgccgacc
     2161 gtgtcgtcgt cttggaggcg aaccgccatt cggcgcaggt ccgcgtgaag gcgcagtggc
     2221 ccgagcacga cggccccgta caacccctgc gcccgggaga agtgatccaa acgcaacagg
     2281 ccgccttcgc cgacatgatg aacggcagca tccgcatctt cccgcagctg acggacggcc
     2341 ggaccatcga ggatgatgag cacgtcctgg cagcaggcta tcgctcggcc ctttatctgc
     2401 ccctccccga ccacacgctg gggccttgca tcgtcgtcgc gctcagtcgc aacccgcggc
     2461 aatacgacga ggcgaccgtg tcgcagctcc gtatggcgct gcttccggcc acgggcgtgg
     2521 cggaggggcc ttcgccctcg aggcagccga tcgatgatcg cctgcagctc gcgatcgaca
     2581 tcgggtctgg gccgattgtc gtgctcgacc cggagagctt ccaggttcgg ctcctgaaca
     2641 acgaggcgga ccgctacttc ggctacggcc gcaacgagat gctgggccgc tctatcgtcg
     2701 aactggtcga aggaggcgtc gatctcatcg attcggtccg gcgcgggcgt tcgcacgtcg
     2761 agcccgtgtc actccggaca gcgggtgggc gcaagcccgc gcgtctcgac ttcatccagc
     2821 atcggaccgg tgacagcgaa gtcctgctcc tcatgatccg cgacctgagc aaggcctacc
     2881 agcgcgtcga gtacttcggc gcgctgaacg ccgtcgccga tcgagccgag gcggcaagcg
     2941 gcccggccga ggcagcgcgc gccatcgtcg aggcgctcaa cgagctcgca ggctcctacg
     3001 ccatctacga gcccgcagac gatggcttcg ccgtggccgc cacgaacctc ccgcctgggg
     3061 tcgacctgcg ggagctgccg ggactcttcc cgggcgcgtc gcgcgaggct gtctacgccg
     3121 agttgccggc gtcccttacg aagcttgtgg acccggcagc agcgcgggag ctcggcgtca
     3181 tcgtcgctcc cgtgaagatc gaggactcgg tcaaagccgt cgtggcgctc gtctcgagca
     3241 atctcggcga gctcgatcgc tccgcggttc agggcttcgc ggcacgcctc gccctggccc
     3301 tgcgcgagag ggcgaccgcg gcggaggccg cagcactcag cgtgccggcc atcgacatgc
     3361 cgagcgaggt ggcgagcgcc gtcatcgatg ccacagacta ccaaacgctg ctgaccggcg
     3421 ccctccgcgc cctctcgcac cacgcgccgt tcgaggtcgc cgtgctgcgc acttgctcgg
     3481 ctggccgcga gacgatccgc atcgtctcgg agcggccgct tggcgtcgac atcattgcgc
     3541 gcctcgtcga gatctcgga
//
LOCUS       DATMBB010000343         3659 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_10459, whole genome shotgun sequence.
ACCESSION   DATMBB010000343 DATMBB010000000
VERSION     DATMBB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10459"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VNL92_07415"
     CDS             complement(<1..505)
                     /locus_tag="VNL92_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWO94576.1"
                     /translation="MPRDPQRPELVRTIRVRNDNQIGVLATVLSTLAREGVSIGEIRI
                     VRSGATSVVRDLDVIADALEQIDRVVAALSQIPQTRVLEVRDEVFAAHAGGKLRMVPK
                     LAIESEADLGRIYTPGVGEVCRRIYEDPASADLYTTIPNSVAIVSDGSAVLGLSNLGA
                     IAAMPVVE"
     gene            589..1206
                     /locus_tag="VNL92_07420"
     CDS             589..1206
                     /locus_tag="VNL92_07420"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWO94577.1"
                     /translation="MTDEEPAPRKGALLYTGKGDAGETGLLGGERVPKWDVQPQAYGA
                     IDEASSAIGLARALASSEETKEALVAAQRALYRIMAELATSPEVELDADMRISEADVR
                     QLEAQTDAFGARVSLGREFVLPGETTAGAALDLARTTVRRAEREAARLAHERGIGGAA
                     VLAYVNRLSSLLFVLERFEDQLQGREARPARIAGQPKRTARNRTK"
     gene            1252..1755
                     /locus_tag="VNL92_07425"
     CDS             1252..1755
                     /locus_tag="VNL92_07425"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HWO94578.1"
                     /translation="MAGRYLFDTLAHVGATSIGEPGSRLFYLIVGRDDGWVRMWLEKE
                     QLRALGDGLDALLRQVGGGGRARDANAAPPPDAPMVGEFRVVRMAVGYDEVQGLVVFT
                     AHDESDTDDSPTLVGRFTREQAAHLAAQAIDVCEAGRPLCAMCGEPIDPDGHFCPRAN
                     GHHPTGL"
     gene            1752..2570
                     /locus_tag="VNL92_07430"
     CDS             1752..2570
                     /locus_tag="VNL92_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HWO94579.1"
                     /translation="MMLDRDELDQYARYQARAIQRLLASRPSDWYWDPTRPDVATFMH
                     EAEFVDCDVIPSGSNWTYLVTLSHPKEGPGLAVYKPRDGEAPLWDFPFGTLYLRELAA
                     YELSRLLGWDIVPPTIVRRGPNGIGSLQLFIQADLAANFFTLRRERPDEFRRFAVFDF
                     LANNADRKGGHCLLDANDGRVWGIDHGLTFHQQWKLRTVIWDYRGEPIPDDLVVAVAR
                     LAQRMEESPHDFDALYACLSQAEQQAFRARVRALLDEPCFPLPRHYRSVPWPPI"
     gene            complement(2586..3491)
                     /locus_tag="VNL92_07435"
     CDS             complement(2586..3491)
                     /locus_tag="VNL92_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HWO94580.1"
                     /translation="MEAGLPSPEDLALIRSEAARLAGEAGELVTEYFRRPLEVRFKSD
                     KGDSDPVTQADEASERFLREAILSRFPGHAVLGEEGRDVAGDASSAWVWVLDPVDGTT
                     NYLNGLALFGVSVGVLHLGRPVAGAIYLPSGVVAAPVVVHAAVGQGAACADEPIVRED
                     RAGSGARIGGVPGGGMRRFRGPAPAGVRLGEPRVLGSISYEMALVALGVLQYAIFARP
                     RIWDVAAGAVIVREAGGSVLTQQAGRWAPLDGFGTSSSAADGLDLREWRAPVVAGDGD
                     VAAYLTMSIPQPPGLVRRMLSRFGR"
BASE COUNT          630 a         1261 c         1190 g          578 t
ORIGIN      
        1 cctccacgac aggcatcgcc gcgatggccc cgaggttgct caaaccaagc acggcggaac
       61 catcgctcac gatggcgacg ctgttcggaa tcgtcgtgta caggtccgcc gatgcagggt
      121 cctcgtagat gcgacggcag acttccccga cgccgggggt gtagatccgg cccagatcgg
      181 cctcactctc gatggcgagt ttcggaacca tgcggagctt gccacccgcg tgggccgcga
      241 aaacttcgtc gcgcacctcg agcacgcgcg tctgcgggat ttgactgagc gcggccacaa
      301 ctcggtcgat ctgctcgagc gcgtctgcga tcacatccaa gtcgcgcacg acagaggtcg
      361 caccggaacg aacgatacgt atctccccga tgctgacgcc ctcgcgtgcg agcgtgctca
      421 ggaccgtcgc caggacgccg atctggttat cgtttcgaac gcggatcgtt cgaacgagct
      481 ccggccgttg tgggtctcgt ggcatagttg cggtgcctcc gcgacacccg atgggcgtat
      541 tctagcatcg accattgacc gcaagccgca cggacaagga gaggagcaat gaccgacgaa
      601 gagcccgcac cgaggaaagg ggccctcctc tacaccggca agggcgacgc cggcgagaca
      661 ggactgctcg gtggcgagcg cgttccgaag tgggacgtgc agccgcaggc gtacggcgca
      721 atcgatgagg cctccagcgc cattggtctg gctcgcgctc ttgcctcgtc ggaggagacg
      781 aaggaggcgc tcgtggcggc ccagcgagcc ctctatcgca tcatggcgga gctggcgacg
      841 agccccgagg tggagctcga tgcggatatg cgcatcagcg aggccgacgt tcggcaactc
      901 gaggcgcaaa ccgacgcctt tggcgctagg gtcagcctgg ggcgagagtt cgtgctccca
      961 ggggagacaa ctgccggcgc ggcgctcgac ctagcgcgaa ccaccgtgcg ccgagccgag
     1021 cgcgaggcgg cacgcctggc gcacgagcga gggatcggtg gcgcggctgt cctcgcctat
     1081 gtcaaccgcc tctcgtcgtt gctcttcgtc ctcgaacggt tcgaggatca gcttcagggg
     1141 cgcgaggcgc gccctgcccg gatcgccggc caaccgaagc gaactgcccg gaatcggacg
     1201 aaatagagcc aacccgttgc cgccggcacg ctggaggtgc aagatcgacg catggcaggc
     1261 cgctatctct tcgacacgtt ggcccacgtc ggcgcgacct cgatcggcga gcccgggagc
     1321 cgcctgttct acctcatcgt cgggcgcgac gatggctggg tgcggatgtg gctcgagaaa
     1381 gagcagctcc gcgccctcgg tgatggcctg gacgcgctcc ttcgccaggt tgggggtggg
     1441 ggccgggcaa gagacgccaa cgccgcgcca ccgcccgacg ctcccatggt cggcgagttc
     1501 cgcgttgttc gcatggccgt gggctacgac gaggtccagg gcctcgttgt cttcacggcc
     1561 cacgatgaat cggacaccga tgatagccca accctcgtcg gccgcttcac acgtgaacag
     1621 gcggcccatc tggcggccca ggccatcgat gtctgcgagg ccggccggcc gctctgcgca
     1681 atgtgtggag agccgatcga tccggacgga cacttctgcc ctcgggccaa tggtcatcat
     1741 ccaacggggc tatgatgctc gaccgcgatg agctcgacca gtatgcgcgc tatcaggcac
     1801 gggccatcca gcgactcctc gcgagccgtc cctcggattg gtactgggac cccacgcgcc
     1861 ccgacgtcgc gacgttcatg cacgaggcgg agttcgtcga ctgcgacgtc atccccagcg
     1921 gctccaactg gacgtacttg gtgacgctca gccatccgaa ggaaggtcca ggtctcgccg
     1981 tgtacaagcc acgggacggc gaggcgccgc tctgggactt cccattcggc acgctgtacc
     2041 tacgagagct cgccgcctac gagctgagcc gcttgctcgg atgggacatc gtaccgccga
     2101 ccatcgtccg ccgcgggccc aacggcatcg gatcgctgca gctgttcatc caggccgacc
     2161 tcgctgcgaa cttcttcacg ttgcgccgtg agcgaccgga cgagttccgc cgcttcgccg
     2221 tcttcgactt cctcgccaac aacgccgacc gcaagggtgg ccactgcctg ctcgacgcga
     2281 acgatggccg ggtctgggga atcgatcacg gcctcacgtt tcatcagcaa tggaagttgc
     2341 gcacggtgat ttgggactat cggggcgagc cgataccgga cgacctggtc gttgcggtgg
     2401 cccgcctggc ccagcgcatg gaggaatcgc cgcacgactt cgatgcgttg tacgcctgcc
     2461 tttcgcaggc ggagcagcag gccttccggg cgcgggtccg tgcgctcctc gacgagccct
     2521 gcttcccact gcccaggcac taccgtagcg tcccctggcc cccgatctaa cgcgacgtgc
     2581 cccgactagc gaccgaaccg ggacagcatg cgccggacga gccctggagg ctgggggatg
     2641 gacattgtga ggtacgcggc gacgtccccg tcgccggcca caactggagc gcgccactcg
     2701 cgaaggtcca ggccgtccgc ggctgacgat gaggtgccga atccgtcgag tggtgcccag
     2761 cgccccgcct gctgagtcaa cacgctgccg cctgcttcgc ggacgataac ggcgcccgca
     2821 gccacgtccc agattcgggg gcgggcgaag attgcgtact gcagcactcc cagggcgacg
     2881 agcgccatct cgtacgaaat gcttccgagc actcgtggct cgccgaggcg tactccggcg
     2941 ggtgctggcc cgcgaaagcg tcgcatgcct ccgccaggca ccccgcctat cctggcacca
     3001 gacccagcac gatcctcccg cacgatcggc tcatccgcgc aggccgcacc ctgtccaacc
     3061 gcggcatgca cgacgactgg ggctgccacc acgccggacg gcaggtagat cgctccggca
     3121 acagggcggc cgaggtgcag cacgccgacc gacacgccga acaacgccag gccgttgagg
     3181 taattcgtgg tcccatcgac tgggtccagc acccacaccc acgccgacga cgcgtcgccc
     3241 gccacgtccc ttccctcctc gcccagaacg gcgtggcccg ggaaacggct gagaatcgct
     3301 tcgcgcagga atcgctccga ggcctcgtcg gcttgggtca ccgggtcgct gtcgcccttg
     3361 tccgacttga accggacctc gagcggacgc cggaagtact cggtaacgag ttcgcccgcc
     3421 tcgcccgcaa gacgggcggc ctcactccgg atgagcgcca ggtcctctgg ggatggaagg
     3481 ccggcctcca tcgctccacg gtaccacggc cgaaacttcg gcgatcgcgt gctatcatgg
     3541 cggcagcttc gcggggagca gctcgtgcgt atattcgacg tccacgttca cttcccacgt
     3601 aattgggaat cggacgaccc gaccgagggc atagaagagc ggatggatca tcttgccga
//
LOCUS       DATMBB010000344         4471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_532852, whole genome shotgun sequence.
ACCESSION   DATMBB010000344 DATMBB010000000
VERSION     DATMBB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532852"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNL92_07440"
     CDS             complement(<1..237)
                     /locus_tag="VNL92_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWO94581.1"
                     /translation="MTNSLESSRILVTGGAGLVGSHIVDRLAAEDVAEVIVLDNFVRG
                     RIENLVGAQARLRQLTIVEADIRDTEAVKAAMKGV"
     gene            complement(234..1373)
                     /locus_tag="VNL92_07445"
     CDS             complement(234..1373)
                     /locus_tag="VNL92_07445"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWO94582.1"
                     /translation="MSVPFVDLGRLHREHRTALLAAFARVLDSGHFVQGQEVEAFEQQ
                     FAHACGAPYAVAVSNGTAALHLALLARGIRPGDEVITVANTFVATAEAIVQAGARPVF
                     ADIDPETMNIDPAEVERRITPRTTAIVAVHLYGRVAQMDALLDIARRYHLALIEDACQ
                     AHGASFNGRPAGTIGDAGCFSFYPTKNLGGIGEGGMILTADRHIALRAASLRDHGQSV
                     RYHHAALGYNYRMSELQAAALSVLLPELYRWNARRSMAAERYAARLAGADLVLPSVEK
                     RDNHVYHLYVVRSHQRNELRSHLAERGIQTAVHYPIPIHLQPAFADYSEGPDSLPHTE
                     AATSKILSLPMHPTIAAEEVDAVCDAILDFDSSLEGRTLAAVSAS"
     gene            complement(1370..2434)
                     /locus_tag="VNL92_07450"
     CDS             complement(1370..2434)
                     /locus_tag="VNL92_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWO94583.1"
                     /translation="MVTALSQPQSIANNTVEPVRVGIVGYGYWGPNLARNVASEPLTE
                     LVAISDMNPARLDAARATHPLARLTTRARDVLEDPTIEAVVIATPLTSHYGLAMEALA
                     NGKHVLVEKPLAACVEEAAELVREARRRDLHLLVDHTFLFTGAVRKMHEYITRGRLGD
                     VYYYDSTRINLGLFHHDTNVIWDLAPHDVAIMLHLLDQPIRSVSAVGARHVDRNVENI
                     AYLTVRFDGPLLAHFHVNWLAPAKVRKTIIGGSERMLIYDDMEASEKLKVYDSGAHLP
                     ETPEGIHRSLVEYRTGDVVAPKLEKAEALAIECAHLARVVRGFEDPVSDGLLGLKVVR
                     VLEAAQRSLECGGMPMEVVL"
     gene            complement(2530..3843)
                     /locus_tag="VNL92_07455"
     CDS             complement(2530..3843)
                     /locus_tag="VNL92_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94584.1"
                     /translation="MILFKHPGRRLAILAVVVALVAGAAVGVWQLFGDDEGPTPVPGS
                     VEPPSLRDAPLREGWPDSLELGTASGLGFADEFDDVEGLRFRYQYLAGGVQNSWRAWE
                     DGEFVHRYVEESAQRDLIPVFTYYMLFQSSPGGSEGDANFASLNDRETMRRYFQDVIA
                     LFEQASAHNDLPIVVHMEPDLWGQMHTRSRDDDASTVPAVVGSTGLPELEGLPDTFAG
                     FGQAVVRLRDMYAPDVLLAYHLSVWGVGSDIVYEDPPDDTVAEYARRSADFYHSIDAD
                     FDLVFSEFSDRDAGFKEHVYGDGGASWWDESDFHRNLVFLSTFVEQTKRRIVMWQIPY
                     GNTQMRTLDNTWNHYQDNRVEWLLGEERDEHLRAYAEAGVIALLFGRGADGATDASDA
                     AGDGVTNPEPINGNTSRSALADDDGGLFRSLAKAYYESGPLALPS"
     gene            4019..>4471
                     /locus_tag="VNL92_07460"
     CDS             4019..>4471
                     /locus_tag="VNL92_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWO94585.1"
                     /translation="MSTRTAAAYPPRPEATLAASKVRSASRPIVRTALALLSAQGLSW
                     LTSLLSILFIPRFLGASDYGLYASVQTGVFLLIAIATFGTTNHIVKSVARAPSHTSNI
                     VLHAALGRLGIWSLFFVGALFLGPLLLGTGRTYSVLIAMVAGAAFHLLM"
BASE COUNT          755 a         1410 c         1523 g          783 t
ORIGIN      
        1 gacgcccttc atggcggcct tcaccgcctc ggtatcgcgt atgtcggcct cgacgatggt
       61 gagctggcgc aggcgcgcct gggcgccgac gagattctcg atgcggccgc gcacgaagtt
      121 gtcgagaacg atgacctcgg cgacgtcctc cgcggccagc cggtcgacga tgtgcgaacc
      181 gacgaggccg gctccgcccg tgacgagaat gcggctcgat tcgagcgagt tcgtcatgat
      241 gcgctcaccg ccgccagggt tcgcccttcg agggacgaat cgaaatcgag gatggcatcg
      301 cagacggcgt cgacctcctc cgcagcaatc gtgggatgca tgggcagcga gaggatcttg
      361 ctcgtagccg cctccgtgtg tggtagcgaa tcagggcctt cgctgtaatc ggcgaaggcc
      421 ggctgcaagt ggatggggat cgggtaatgg acggcggttt gtatgccgcg ctccgccagg
      481 tgagagcgca gctcatttcg ctggtgagag cgaacgacgt acaggtgata gacgtggttg
      541 tcgcgcttct cgacgctcgg caggacaagg tcggcgccgg cgagcctcgc cgcatagcgc
      601 tcggccgcca tggaccgccg cgcgttccat cggtaaagct ccggcagcag tacgctgagc
      661 gccgccgcct ggagctcgga catgcggtag ttgtaaccga gcgcggcgtg atggtaacgc
      721 acggactgtc cgtgatcgcg cagcgaggcg gcccgcaagg cgatgtgtcg atcggccgtg
      781 aggatcatgc cgccttcacc gatgccgccg aggttcttcg tggggtagaa gctgaagcag
      841 ccggcgtcgc cgatagtgcc agctggcctc ccgttgaacg aggcgccgtg cgcttggcat
      901 gcgtcctcga tgagtgccag gtggtaccgc cttgcgatgt cgagcagcgc gtccatctgc
      961 gccacgcggc cgtacaggtg gacggcgacg atagctgtcg tgcgtggcgt gatgcggcgc
     1021 tcgacttccg cagggtcgat gttcatcgtc tccggatcga tgtcggcgaa aacgggccgc
     1081 gcccccgctt ggacaatcgc ctcggcggtt gctacgaagg tgttcgcgac cgtgatgact
     1141 tcgtcgccgg gccgaatgcc gcgcgcgagg agcgccagat ggagggccgc ggtgccgttg
     1201 ctcactgcga ccgcatacgg ggcgccgcaa gcatgggcaa actgctgctc gaacgcctcg
     1261 acctcttgtc cctggacgaa gtggcccgaa tcgagcactc gcgcgaaggc ggcgagcaag
     1321 gctgtgcgat gctcgcgatg cagccggccg aggtcgacaa agggaacgct cacaaaacca
     1381 cctccatcgg catgccgccg cactcgaggg agcgctgcgc ggcttccaac actcgcacaa
     1441 ccttgagccc gaggagacca tcggacacgg ggtcctcgaa cccacggacg acccgggcca
     1501 agtgagcgca ctcgatggcc agcgcctcgg ccttctcgag cttcggcgca accacgtcgc
     1561 ctgttcggta ctccaccagc gaccgatgga tgccctccgg ggtctcaggc agatgggccc
     1621 cggagtcgta gaccttgagc ttctcgcttg cctccatgtc gtcgtagatg agcattcgct
     1681 cgctgccgcc aatgatggtc ttgcgcacct tggcgggggc caaccagttg acgtggaagt
     1741 gcgcgagcag cggcccatcg aaccggaccg tgaggtaggc gatgttctcg acattgcggt
     1801 ccacgtgacg cgctccaaca gccgacacgc tccggatcgg ctggtcgagc aggtgcagca
     1861 tgatcgcgac gtcgtgcggc gccaggtccc agatgacgtt cgtatcgtga tggaagaggc
     1921 ccaggttgat gcgggtcgaa tcgtagtagt agacgtcgcc gaggcgcccg cgcgtgatgt
     1981 actcatgcat ctttcgaacg gccccggtga agaggaacgt gtggtcgacg agcagatgca
     2041 ggtcgcgccg gcgcgcctcg cgtacgagct cggcagcctc ttcgacgcac gccgcgagcg
     2101 gcttctccac cagaacatgc ttgccgttcg cgagcgcctc catcgccagc ccgtagtgac
     2161 tcgtgagcgg ggtcgcgatg acgacggcct ctatcgtcgg gtcctccagc acgtcgcgcg
     2221 cgcgggtcgt gagacgcgcg agcgggtgcg tggcccgtgc cgcgtcgagc cgggccggat
     2281 tcatgtcact gatggcgacg agctcggtca ggggctcact cgcgacatta cgggcgaggt
     2341 tcggccccca gtagccgtag ccgacgatgc cgacgcgcac gggttcaacg gtgttgttgg
     2401 cgatggattg gggctgcgaa agtgccgtga ccatgcaacg atgttcggcg ccgtgcgtgg
     2461 aggtggagtg aaggggaagt tgtcgcccgg ttcacctccg cgacgaatcg ttgccgtgag
     2521 tcggggaagc taggacggga gggcgagtgg gccggattcg tagtaggcct tggccaggga
     2581 ccggaacagc ccaccgtcat cgtccgctaa ggcggagcga gaagtgttgc cgttgatggg
     2641 ctctgggttc gtgacgccgt cgcccgccgc atcgctcgcg tcggtcgcgc cgtcggcgcc
     2701 gcgtccgaac agcagcgcga tgacaccggc ctccgcgtag gcccggaggt gctcatcccg
     2761 ctcctcgccg aggagccatt cgacgcggtt gtcctggtag tgattccacg tgttgtcaag
     2821 cgtccgcatc tgcgtgttgc cgtacggaat ctgccacatc acgatgcgtc gcttggtctg
     2881 ctcgacaaag gtgctgagga acacgaggtt gcgatggaaa tcggactcgt cccaccagga
     2941 ggcgccgcca tcgccgtaga cgtgctcttt gaatcccgcg tcgcgatcgg agaattcgct
     3001 gaacacgagg tcgaagtcgg catcgatgga gtggtagaaa tcggcggaac gccgcgcata
     3061 ctcggccacg gtgtcatccg gcgggtcctc atagacgatg tcgctgccga caccccagac
     3121 gctaaggtgg taggcaagca ggacgtccgg cgcgtacatg tcgcggagac gcacgacggc
     3181 ctgtccaaag ccggcgaacg tgtcgggcag cccctcaagt tcaggaaggc cggtcgaccc
     3241 gactaccgcg ggcactgtgg aagcatcgtc gtcgcgcgag cgagtatgca tctggcccca
     3301 caggtcgggc tccatgtgca cgacgatcgg aaggtcgttg tgcgccgaag cctgctcgaa
     3361 gagggcgatg acgtcctgga agtagcgccg catggtctcg cggtcgttga ggcttgcgaa
     3421 gttggcgtca ccttcggacc cgcccggcga ggactggaag agcatgtagt acgtgaatac
     3481 ggggatgagg tcgcgttgcg ccgactcctc gacataacgg tggacgaact cgccgtcctc
     3541 ccaagcgcgc cagctgttct gcactccgcc ggcgagatac tggtagcgga atcgaaggcc
     3601 ctcgacatcg tcgaactcgt cggcgaagcc gagtcccgac gccgtgccaa gttcgagcga
     3661 gtccggccag ccttcacgga gaggagcatc gcgtagggaa gggggctcga ctgagccggg
     3721 gaccggcgta ggcccttcgt catcgccgaa cagctgccag acgcctaccg ccgctcctgc
     3781 gacgagcgct acgacgaccg cgaggatggc gaggcgccgc ccaggatgct tgaacaggat
     3841 catcgagtcc acctccctgc acagctacga tatggacctg tggtccagcg atgatggaca
     3901 tcgcgtggcc gatcggttga gacgactggg accatgggtc gattcgacga gcggcctcga
     3961 ccggtcgtcc acgccggctc taccgcggct tgacggttgc tcgccacact gggaaccgat
     4021 gtcaacccga accgccgcgg cctacccgcc ccgccccgag gcaacgctcg cggcgtcgaa
     4081 ggtacgttcg gcatcacgcc cgattgtgcg gacggcgctt gcgctgctct cggcccaagg
     4141 cctgtcttgg ctgacttcgc tgctcagcat cctcttcatc ccgcggttcc tcggagcgag
     4201 cgattacggc ctctacgcct cggtgcagac gggagtcttc ctgctcatcg ccatcgcgac
     4261 gtttggtacg acgaatcaca tcgtcaagtc ggtggctcgc gccccctccc acacgtcgaa
     4321 catcgttcta cacgcggccc tgggccgcct cggcatctgg agcttgttct tcgtgggggc
     4381 gctctttctg gggccgctcc tgttggggac gggccggacg tactccgtcc tcatcgcgat
     4441 ggtggcgggg gcagcattcc acttgctgat g
//
LOCUS       DATMBB010000345         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_378719, whole genome shotgun sequence.
ACCESSION   DATMBB010000345 DATMBB010000000
VERSION     DATMBB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378719"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2172)
                     /gene="mutS"
                     /locus_tag="VNL92_07465"
     CDS             complement(<1..2172)
                     /gene="mutS"
                     /locus_tag="VNL92_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWO94586.1"
                     /translation="MTTPIRRQYLEVKARHPDAIVLFRLGDFYEAFDDDAHICARELD
                     LTLTSKPMGKGLRVPLAGIPHHALDGYVSRLVARGHRVAICEQLADPSAAKGLVPRDV
                     VRVVTPGTVLESSVLEGRTNAYLVAVVAGEGDEAGVAWADVSTGEFVTMQLALERVGI
                     ELARLQPSELLLPPGAESLVADVAAAASHVEPLSHRDAEAMLLEHFGSSTLDAFGCAG
                     RPLAVQAAATVLAWVKRVAPSAAPSLARLATRPAISGLVLDAATRRNLELFPRAEAPG
                     ASLLETLDLTCTAKGARLLRRWLGEPLCDVPAIVERQDAVALFFEDALLRGEVVSLLR
                     ALPDVERLAGRTAAGYSSPREVAALRRGLEVIPQVIGALGEHARRFPNLRALPDVAQA
                     IAATLVDDPPLTLDEGGVVRDGVSPDLDRLRAIARDARAGLAELEAAERERTGIRSLR
                     VGYQRVFGYYIEVSNAQRSLVPDDYEPRQTLTNCQRYVTPALRTYETQVLEARERIVQ
                     LETDVFRRLCSQLGGEVERIRSVSDALAEIDVYVSFAEAAARYGYVRPEVQEDGPLVI
                     REGRHPVIERRLPPGAFVPNDTMLDAETCQIVVITGPNMAGKSTYLRQVALIALMAHM
                     GAYVPASAATIGLIDRIFTRVGAQDELAAGRSTFMVEMVETATILNQATSRSLVPDDY
                     EPRQTLTNCQRYVTPALRTYETQVLEARERIVQLETDVFRRL"
     gene            2262..>3227
                     /gene="hflX"
                     /locus_tag="VNL92_07470"
     CDS             2262..>3227
                     /gene="hflX"
                     /locus_tag="VNL92_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWO94587.1"
                     /translation="MSTAAIQDRAFLVGVEARGAAHWRIDDSLDELAQLAETAGAVVV
                     GKASQRLEHPNPATYIGKGKLQEIVSARSSAGYTTVIFDDELSPSQQRNLENALGVKV
                     LDRTALILDIFARRAHTREGVLQVELAQLEYLLPRLAGQWSHLERMEGAIGTRGPGET
                     QIETDRRLARNRIAHVRRQIEDVRRHRALHRKRRAKQGMAVIALVGYTNAGKSTLMRA
                     FSGADVLAEDRLFATLDPVTRRISLPEAGAALLTDTVGFIQKLPAQLVAAFRATLEEL
                     NEADALLHVVDVTHSAAADQAATVERTLIDLGLQDKPRVTALNKAD"
BASE COUNT          548 a         1104 c         1096 g          479 t
ORIGIN      
        1 gagccggcgg aatacgtccg tctcgagttg cacgatccgt tcgcgcgcct cgagcacctg
       61 cgtttcgtac gtgcgcagtg cgggcgtcac gtagcgctgg cagttcgtga gcgtctggcg
      121 tggctcgtag tcgtccggca caagcgagcg ggaagtggcc tggttgagga tggtcgccgt
      181 ctcgaccatc tcgaccatga aggtcgagcg accggccgcc agctcgtcct gcgcccccac
      241 gcgcgtgaag atgcgatcga tgagtccgat ggtggccgcc gatgccggaa cgtacgcgcc
      301 catatgcgcc atcagggcga tgagcgccac ctggcgcagg taggtcgatt tgccggccat
      361 gttggggccc gtgatgacga cgatctggca cgtctcggca tcgagcatcg tgtcgttggg
      421 aacgaacgcc ccaggcggaa ggcggcgctc gatgacggga tggcggccct cgcgaatgac
      481 gagcggcccg tcctcctgaa cctcgggccg aacgtagccg tagcgcgccg ccgcctcggc
      541 gaacgagacg tacacgtcga tctcggcaag cgcatcggaa acgctccgga tacgttcgac
      601 ctcgccgccc agctgcgagc agagccggcg gaatacgtcc gtctcgagtt gcacgatccg
      661 ttcgcgcgcc tcgagcacct gcgtttcgta cgtgcgcagt gcgggcgtca cgtaccgctg
      721 gcagttcgtg agggtctggc gtggctcgta gtcgtccggc acaagcgagc gctgcgcgtt
      781 gctgacttcg atgtagtacc cgaacactcg ctggtaccca acccggagcg atcgaatgcc
      841 ggttcgctcg cgctcagccg cctccagctc cgcgagtccg gctcgggcat cccgcgcgat
      901 cgcgcggagc cggtcgaggt cgggcgagac gccgtcgcgg acgacgccgc cctcgtccaa
      961 tgtaagcggc ggatcgtcga ccagcgtggc ggcgatggcc tgcgccacgt ccggaagagc
     1021 gcggaggttg ggaaaccgcc tggcgtgctc gccgagcgcc ccgatgacct gcgggataac
     1081 ctcgaggccg cgacgcagcg cggccacctc gcgcggcgac gagtagccgg cagcagtgcg
     1141 cccggcgagg cgctccacat cgggcaatgc gcgcaggagc gaaacgacct cgcctcgaag
     1201 cagcgcgtcc tcgaagaaca gcgccacggc atcctgccgc tcgacgatgg ccgggacgtc
     1261 gcacagcggc tcgccgagcc agcgtctcag cagtcgcgcc cctttcgccg tgcacgtcag
     1321 gtccagcgtt tccagcagcg atgcccccgg cgcctccgcc cgcgggaaca attcgaggtt
     1381 tcgccttgtc gctgcatcaa gaacgaggcc cgagatcgcg ggacgggtag ccaagcgcgc
     1441 gagcgacggg gccgccgacg gtgccacacg cttgacccag gccagcacgg tcgccgcagc
     1501 ctgcacggcg agcggacgcc cggcgcatcc aaacgcatcg agcgtgctgc tcccgaaatg
     1561 ctccagcagc atcgcctcgg cgtcgcgatg ggacagcggc tcgacatggg aggctgccgc
     1621 cgcgacatcg gcgacaaggg actcagcccc ggggggaaga agcagctcgg acggttgcag
     1681 ccgcgccagc tcgatgccga cacgctcgag cgctagctgc atggtcacga attcgcccgt
     1741 gctgacgtcc gcccaggcca ctccggcctc atctccctcg ccggcgacga cggcgacgag
     1801 atacgcgttc gtgcgcccct cgaggacgct gctttccaga accgtgcccg gcgtaaccac
     1861 ccgcacgacg tcgcgcggga cgagcccctt cgcagcggag gggtcggcaa gctgctcaca
     1921 tatcgcgact cgatgtcccc gggcgacgag ccgcgatacg tagccatcga gggcatggtg
     1981 ggggatgcca gccaggggaa cgcggaggcc cttgcccatg ggcttcgacg tgagcgtcag
     2041 gtcgagctcg cgggcgcaga tgtgggcatc gtcgtcgaag gcctcgtaga agtcgccgag
     2101 gcggaacagg acgatggcat cggggtgacg agccttgacc tcgagatact ggcggcggat
     2161 cggtgtcgtc acgcggcatc cctgggtttt gcgtcagtat agccccggcc gtcccgtgct
     2221 acgatatcta tggaggacca accatcgcac ggaacgcatc catctcgacc gcggcaatcc
     2281 aggatcgagc ctttctcgtg ggcgtcgagg cccgcggagc ggctcactgg cgcatcgacg
     2341 actcgctcga cgagcttgcc cagctcgcgg agaccgcagg cgcggtagtc gtcggtaagg
     2401 cgtcccagcg gctcgagcat ccgaaccccg cgacgtacat tggcaagggc aagctgcagg
     2461 agatcgtctc cgcacgaagc tccgctggct acaccaccgt catcttcgat gacgagctgt
     2521 caccatcgca gcagcgcaac ctcgagaacg cgctgggcgt gaaggtgctc gatcgcacgg
     2581 ccctcatcct ggacatcttc gcgcgccgcg cccacacgcg agagggcgtg ctgcaggtgg
     2641 agctagccca gctcgagtat ctgctgcccc gcctggccgg acagtggagc cacctggagc
     2701 ggatggaagg cgccatcgga acgcgcggcc ccggcgagac gcaaattgag accgaccggc
     2761 gtctcgcccg gaatcggatc gcgcacgtcc gccgccaaat cgaggacgtg cggcggcatc
     2821 gcgccctcca tcgcaagcgg cgagcgaagc agggcatggc ggtgatcgcg ctcgtcggct
     2881 atacgaacgc cggcaagagc acgttgatgc gggcgttctc gggcgccgac gttctcgccg
     2941 aggatcggct ctttgcgacg ctcgacccgg tgacacgacg gatctccctt cccgaggcag
     3001 gtgccgcgct gttgaccgat acggtgggat tcatccagaa actccccgcc cagctcgtcg
     3061 ccgcctttcg cgccacgctc gaggagctga acgaggcgga cgcgctgctc catgtcgtcg
     3121 atgtgacgca ctcggccgct gccgaccagg ccgccacggt agagcgaacg ctcatcgatc
     3181 tcggcctgca ggacaagccc cgggtcacgg cgctgaacaa ggccgat
//
LOCUS       DATMBB010000346         2207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_254944, whole genome shotgun sequence.
ACCESSION   DATMBB010000346 DATMBB010000000
VERSION     DATMBB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254944"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VNL92_07475"
     CDS             complement(<1..886)
                     /locus_tag="VNL92_07475"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94588.1"
                     /translation="MTWPSGPSATRRRTVRVALDVMGGDRPPEERVRGGLAALDDEDI
                     ELILVGVPDVVEQALREHDISRRSVRVVPSGPAIPEGTSPVSVLRERADAAVAVATRL
                     VKAGEADAVASAGNTGASMASAQVILGTLPGVDRVAAGGPIFGPLAPQTVLLDGGANV
                     DVRPHQLVAFGVLGAGYVREAFQIENPTVALLANGAERGKGNKQVREAYPLFEASGLN
                     FIGQIEGYDLLGGRANVVICDGFVGNILVKFWEVVVQAVVRYAIAEAGELVPPASAQR
                     MVEAAHAISGELTVRGGPL"
     gene            complement(960..1412)
                     /locus_tag="VNL92_07480"
     CDS             complement(960..1412)
                     /locus_tag="VNL92_07480"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWO94589.1"
                     /translation="MTAREPEPTTLYFAYGSNLDGEHMAQLCPGAEAIATARAEGYRF
                     RINQAGYATLVPEEGATAYGLVWRVTPDHRRALDWYEEVEAGLYVPEIIEVELRDSAA
                     ERSLVYIATDSTPGVPAIAYMQGILRAARALGFPGSYVAELASWRPGQ"
     gene            complement(1409..>2207)
                     /locus_tag="VNL92_07485"
     CDS             complement(1409..>2207)
                     /locus_tag="VNL92_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWO94590.1"
                     /translation="VRRTAVAFFATLDDATRTVAAIGHAGYLPVTLELMDSVCINVVE
                     DHAALGLRRDAEALLILETDGSHDAVTLEELEAIAEVCRQNGSLEVRLATSNAEREAI
                     WAARRSINSALARVRPSRLGEDIVVPRANVPDLVRRIRSISDETGLPIAVFGHAGDGN
                     MHPNILFDRSREGELARVEAAAAGIFRAALALGGTLSGEHGIGTLKKAFLEEDLGPEA
                     VDVMRSIKQALDPNGILNPHKVFPDAPGEARSGFLTALPTLEGHVPG"
BASE COUNT          342 a          756 c          770 g          339 t
ORIGIN      
        1 ggagcggccc gccgcgcacg gtgagctcgc cgctgatcgc gtgggcggct tcgaccatcc
       61 tctgcgcgga agcgggcggc acaagctcgc ccgcttccgc gatggcgtag cgcacaacgg
      121 cctgaacgac gacctcccag aacttcacga ggatgttgcc gacgaatcca tcgcagatga
      181 cgacgttcgc acggccgccg agcaggtcgt agccctcgat ttgcccgatg aagttgagcc
      241 cgctggcctc gaagagcggg tacgcctcgc gtacctgctt gttgcccttc ccgcgctcgg
      301 ccccgttggc gagcaatgcg acggtcggat tctcgatctg gaacgcttcg cggacgtagc
      361 cggctcccag cactccgaac gcgacgagct ggtggggccg gacatcgacg ttcgcgcctc
      421 catccagcag cacggtttgg ggcgcgagcg gaccgaaaat cggcccgccg gcagcgacgc
      481 gatcgacgcc gggcagggtg ccgaggatga cttgcgccga tgccatgctt gcgccggtat
      541 tgcccgcgct ggcgactgca tcggcctcgc ccgctttgac gaggcgcgtg gctaccgcca
      601 cggccgcatc ggcgcgctcc cgaagcacgg agaccggcga tgtaccctcg ggaatcgcgg
      661 gcccggaagg aacgacacgc acgctacgtc gcgaaatatc atgttcgcgg agcgcctgct
      721 cgaccacgtc cggcaccccg acgaggatga gctcgatgtc ctcgtcgtca agcgccgcca
      781 ggccgcctcg cacacgttcc tcgggtggcc tgtcgcctcc catcacgtcg agcgcgaccc
      841 gcacggtacg ccgccgtgtc gcggaaggac cgctcggcca ggtcatcgcc tcacctccac
      901 gcgccccttg gggctgcagg cggaatgtag cacaggcacg taggcgcttg cagggctggc
      961 tattgccctg ggcgccacga cgcgagctcg gcaacgtagc tgccggggaa gccgagcgcc
     1021 cgggcggcac gcaggatgcc ctgcatgtac gcgattgcgg ggacgcccgg cgtgctatcg
     1081 gtcgcgatgt agacaaggct ccgttcggcg gcgctatcac gcagttcgac ctcgatgatc
     1141 tcgggcacgt agagtcccgc ctccacctcc tcgtaccaat cgagggcgcg ccgatggtct
     1201 ggcgtcacac gccacacgag gccgtaggcg gtcgcgccct cctccgggac gagggtcgcg
     1261 tatccggcct gattgatgcg aaagcggtag ccttccgctc gggccgttgc gatggcctcc
     1321 gccccgggac acagctgagc catatgctcg ccatcgaggt tggagccgta agcgaagtag
     1381 agggtggtgg gctccggctc gcgggccgtc atccggggac gtggccctcg agtgtcggga
     1441 gggccgtgag aaagcccgag cgagcttcgc ccggggcgtc cgggaacacc ttgtgtgggt
     1501 tcaggatgcc gttcggatcg agcgcctgct tgatggaccg catgacgtcg acagcctccg
     1561 gccccaggtc ctcctcgagg aacgccttct tcagcgttcc gatgccgtgc tcgccggaaa
     1621 gtgtgccgcc gagcgccagg gcggcccgga agatgcccgc cgcggccgcc tcgacgcggg
     1681 caagctcgcc ctcgcgcgat cgatcgaaca ggatgttggg atgcatgttg ccatcgcccg
     1741 cgtgaccaaa aacggcgatg gggaggcccg tctcgtcgct gatcgatcgg atgcggcgca
     1801 cgaggtccgg cacgttggcg cgcgggacga cgatgtcctc gcccagacgg ctcgggcgca
     1861 cgcgcgcgag ggcgctattg atggagcgcc gcgccgccca gatcgcctca cgctccgcgt
     1921 tcgaggtcgc gagccgcacc tcgagggacc cgttctggcg gcagacctcg gcgatcgcct
     1981 cgagctcctc gagcgtgaca gcgtcgtggc tgccgtcggt ctcgaggatg agcagcgcct
     2041 cggcatcgcg ccggaggccg agcgccgcgt ggtcctcgac gacgttgatg cagacgctgt
     2101 ccatcaactc gagggtgacg ggcagatagc ctgcgtggcc aatggccgcg acggtgcgcg
     2161 tggcgtcatc gagggtggcg aagaacgcga cggccgtgcg ccggaca
//
LOCUS       DATMBB010000347         3040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_327040, whole genome shotgun sequence.
ACCESSION   DATMBB010000347 DATMBB010000000
VERSION     DATMBB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327040"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1491
                     /gene="proB"
                     /locus_tag="VNL92_07490"
     CDS             295..1491
                     /gene="proB"
                     /locus_tag="VNL92_07490"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWO94591.1"
                     /translation="MPDSDHRSSPAAGELRYRRIVAKFGTNLLTAGSERLDLTVMASL
                     VGQVAALRAAGAQILIVTSGAIAAGRHRLGGRRIGKEAPQRQMLAAVGQSRLMQAYDE
                     LFAWHSLVIAQTLLTRRDLADRQGYLNARNTLLGLLDLGVIPIINENDVVAIEEIGER
                     TIGDNDTLSALVANLVDADLLAILTDIGGLYTRDPRRDGDASLIPRVERVDEHIERLA
                     GAAGTARGRGGMLTKVRAAALATASGVDVVIADGRSPHVLPELACGGQLGTLFPATAP
                     RAEGRRRFLLADVTVRGRVVVDEGARRALVAEGRSLLPAGVRNVSGNFERGDAIDICG
                     EDGVRIARGITNYAAPDIEHIMGMRSDRLSEVLGHDYGSEVVHRNNLVLLTNRGEEYG
                     TRAARR"
     gene            1839..>3040
                     /locus_tag="VNL92_07495"
     CDS             1839..>3040
                     /locus_tag="VNL92_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWO94592.1"
                     /translation="MRLARYRPRLPLVVALALAAAAIAWWSPSDAQVPNPSDVADDGF
                     APGEVIVGFEEGALSGQSTDALLAGASVADRSESLGAERVVLPPGVSVEQAISELQDA
                     PGVAFVEPNYLLHASAEPNDPLYPRQQFNLDVIGAPAAWDTNTDASGAILAIIDTGVD
                     LEHEDLAGQLWTNPNEIPGDGIDNDHNGCPDDVHGCNFISPSIGDSCGYQALPPHSDV
                     RDDAAHGTRVAGIAAAAGDNGIGIAGMGWQGRLMIIKAMDCEKSGSSFDVARAIDYAV
                     ANKASIINLSLGGSGRSEMLIQAIDRATNAGVLLVAATGNLGPQVNFPANLPNVLAVG
                     ASGAFDAPDSRAPFSNWGPEVDVVAPGIDVLSTAPIVAASHLAPNQSLLVAAQPPQPQ
                     RLNVVVEDR"
BASE COUNT          510 a         1074 c          939 g          517 t
ORIGIN      
        1 ctgaagggaa ccggggcgag ctggcgcgca gatcgaacat cgatcgtctg gccttcacta
       61 ccttcacctt ccatcatcac gtgacgaggg gtctcccgac caacctgtac atcatcaagc
      121 catctccgct tcccccaaaa agtacgctcc gccgcggaca cgaacaattt acgcggcaac
      181 aaccatcgcg gcaatcgaaa gtcacgccga attcgcagtc gtctccgcgg ttcgtgcctc
      241 acggcgagca ttgctatact ttcgaggaga ctcaacacct gaggatcgca cgagatgccc
      301 gattcggacc accggtcgtc ccccgccgcc ggcgaactgc gctaccgtcg catcgtcgcc
      361 aagttcggca cgaatctgct cactgctggg tccgagcggc tcgatctgac ggtgatggcg
      421 agccttgtcg gccaggtcgc cgcactccga gcggccggcg cgcagatcct catcgtcacg
      481 tccggcgcca tcgcggccgg gcggcaccgg ctcggcgggc gccggatcgg caaagaggcc
      541 ccgcagcgac agatgttggc ggccgtgggc cagagccggc tcatgcaggc gtacgacgag
      601 ctctttgcgt ggcacagcct cgtcatcgcc cagaccctcc tcacccgccg cgacctcgcc
      661 gaccgccagg ggtacctcaa cgcccgtaac accctgctcg gcctgctcga ccttggtgtg
      721 atacccatca tcaacgaaaa cgatgtcgtg gccatcgagg agataggcga acgaacgatc
      781 ggcgacaacg acacactctc ggccctcgtt gcgaatctcg ttgacgcgga cctcctcgcg
      841 atcctgaccg acattggcgg gctctacacg cgcgatcctc gtcgcgacgg cgacgcgagc
      901 ctgattccgc gggtcgagcg cgtcgacgag cacatcgagc ggctggccgg cgccgcgggt
      961 accgctcgcg gacgcggcgg catgctgacg aaggtgcgcg ccgccgcgct cgccaccgcg
     1021 tcgggcgtcg acgtcgtcat cgccgacggg cgctcaccgc acgtcctgcc cgagctcgca
     1081 tgtggcggcc agctcggcac gctattcccg gcgacagccc cccgcgcgga aggccgccgc
     1141 cgctttctcc tcgccgacgt cacggtccgt ggccgggtcg tcgtcgacga aggtgctcgc
     1201 cgtgccctcg tcgcggaggg gcgaagcctc ttgccggccg gcgtgcggaa cgtgagcggg
     1261 aatttcgaac ggggcgatgc catcgacatc tgtggcgagg acggcgtgcg catcgcgcgt
     1321 ggaatcacca actatgctgc tcccgacatc gagcacatca tgggcatgcg ctccgaccgt
     1381 ctgagcgagg tgctcgggca cgattacggc agcgaagtgg tgcaccgcaa caacctcgtc
     1441 ttgctgacca accgcgggga ggaatatgga acacgagcgg ctcgtcgata gggctcaccg
     1501 cgtagagtcg gaggcgagca cgctcgtgct tgtcgccggc gcgtcgtccc cgttatgggg
     1561 gccgcccgtc gctgctgcgt tgcgctggcc cccccacctc acgatcggtg tctcgttggg
     1621 ccctgccgtc tccctcttgt tcggcggcct gctccacgat ctcctcaaga cccgaacgcg
     1681 cctctcgatg agacgtgtga cgatgctcga aaccatggcc tcctctctcc tcttcgccgg
     1741 agtcttcgtc ctgcccctcg tgcggcacta cgtgccttcg agacggcgcc ggcctgggcg
     1801 agtatcccga atcctgcgga tgcgctaatc tccgtagcat gcgtctcgcc cgataccgtc
     1861 cacggcttcc cctcgttgtt gccctcgccc tcgccgcagc cgccatcgcc tggtggtcgc
     1921 cgagcgatgc ccaggtgccg aacccttccg acgtcgcaga cgatggcttc gcgccgggtg
     1981 aggtcatcgt cgggttcgag gaaggcgcgc taagcgggca gtcgaccgat gctctccttg
     2041 cgggcgccag cgtcgcagac cgaagcgagt cgctgggggc ggaacgcgtc gttctaccgc
     2101 ccggggtctc ggtcgagcag gcgatctcgg aactgcagga tgctcccggc gtcgccttcg
     2161 tcgagccgaa ctatcttctc cacgcctcgg ccgagccgaa cgatccgctt tatccgcgac
     2221 agcagttcaa cctcgacgtc atcggcgccc ccgctgcttg ggacaccaat accgacgcct
     2281 caggcgccat tctcgccatc atcgatacgg gcgtcgacct ggaacacgag gacctcgcgg
     2341 gtcagctgtg gaccaacccg aacgagatac ccggcgatgg catcgataac gaccacaacg
     2401 gctgcccgga cgatgtgcac ggctgcaact tcatctcgcc aagcatcggc gatagctgcg
     2461 gttatcaagc attacctccg cactccgacg tgcgcgacga cgcggctcat ggaacgcgag
     2521 tcgcaggcat tgcagcggcg gcaggcgaca atggcatcgg catagccgga atgggctggc
     2581 agggacggct catgatcatc aaggcgatgg actgtgagaa gtccggatcc tctttcgatg
     2641 tggctcgtgc catcgattac gccgtcgcga acaaggcatc gatcatcaat ctcagcctcg
     2701 gcgggtccgg gcgctcggag atgttgatcc aagccatcga ccgagcgacg aatgcgggcg
     2761 tgctgctcgt tgcggccacc ggcaacctcg gaccgcaggt caacttcccc gccaaccttc
     2821 cgaatgtgct ggctgtcgga gcttcgggtg ccttcgatgc ccccgatagc cgcgcgccct
     2881 tcagcaactg gggcccggag gtcgacgtgg tggcacccgg catcgacgtg ctgagcaccg
     2941 cgccgatcgt cgcggcatcg caccttgcac ccaaccagtc gctcttggtt gccgcgcaac
     3001 ccccccagcc acaacggctg aacgtcgtcg tcgaggaccg
//
LOCUS       DATMBB010000348         2544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_418909, whole genome shotgun sequence.
ACCESSION   DATMBB010000348 DATMBB010000000
VERSION     DATMBB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418909"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VNL92_07500"
     CDS             complement(<1..300)
                     /locus_tag="VNL92_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWO94593.1"
                     /translation="MPIADSVVLGRDVKIFHPSLVNLYGCTIGDGTKIGAFVEIQKNA
                     FIGARCKVSSHTFVCEGVHIGDEVFIGHGVMFTNDIYPRATNEDGSVQTEADWQVV"
     gene            1173..2228
                     /locus_tag="VNL92_07505"
     CDS             1173..2228
                     /locus_tag="VNL92_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94594.1"
                     /translation="MAVPAGDCSWGSLTISKGIHLRGAGAGATNITLSGAITLRKHAT
                     RSVEVSGFTFRRSGGGNSAKMMYVEGPWNGEPPLIHDNVFIASNSGIIRYQTNGGVIY
                     RNTFRGDLDDSALQHKVDGDSESWSTPDTMGTRDTNGKRNLYVEDNVFEGMANQATDF
                     DDAARVVFRHNTLVSSSFNSHGLDTSPIGVRHYEIYENSFRYPNKSVNQSWHIWLRGG
                     TGVIFNNQVEDLNGTQWGNKTELLFSVRAAVDGGVEGCCRSYPCKHQVGQNHDGSSQF
                     TDPVRLWNNSGTLAWGLNSAWNNTCGQNMEDYLQNGRDFIFATSPKSGYTPYPYPHPL
                     RVDTARPLPPSGLTVAP"
BASE COUNT          473 a          851 c          777 g          443 t
ORIGIN      
        1 cactacctgc cagtcggcct cggtttgaac ggagccgtct tcattggtgg cgcgcggata
       61 gatgtcgttc gtgaacatca cgccgtggcc gatgaagact tcgtcgccga tgtgaactcc
      121 ctcacacacg aacgtatgag aggagacctt gcagcgcgcc ccgatgaagg cgttcttttg
      181 aatttcgacg aaggcaccga tcttggtgcc atcgccgatc gtgcagccgt aaaggtttac
      241 gagcgaggga tggaagattt tgacatcccg gccgaggact actgaatcgg caatcggcat
      301 cgctcttgct ccttatcctc gggcggcgac gagccgatcg ccgcccaggc tcgttgttat
      361 ggtcggacgg cggccgttgt atatccggga cacgccccgc ttcgaacgca gacggagacg
      421 accgaggcca tcccagtacg gcgcgatcgt atcctgaccc ctcggcccgt ggaaaggcac
      481 gggctccgga aattgcccca accggatcga gcagcacgct gccgccgcgg ctactccccg
      541 agaagaaggc aaaatcgatc ttcgccgggc cccggattga tcggtcaatc gctatcgtcc
      601 gccgagaatc tggtgcattg aagcaccccc ctcgcgcggc cgccgtgatg tgggtcgagt
      661 ttctgcactt ccggggccgg ccgcccagcc gctcgtttcc gccggcatcg gacgggctgt
      721 cgggaatcga cgactcgagc ggaaccacgc cgcccacgcg ccgcgatgcg ccgattgcgc
      781 cagcttccct cggccaacta agttatcctc acggagcgtc cgcccgggtt ggcaagcggg
      841 caaccgcgcg tgtcgttcgg ccgtctatgc cctcgcgcca cgggtgactg tctctaaact
      901 tctacatgtg gacgcttatc gtgccatgga gtttaatggc agcccaggtc tcgtccggct
      961 ccctcggcgc ggccgtcaat cagggtgcga tacagtagcc atggaggccc agagtcatga
     1021 ctcgaacggt ccagcgtagt aacgttctcg cctgcgcgct gatcgcggcg ctgggcatcc
     1081 tggagcccgc ctcggccgcc acggtcaacg cggcgagctg ccaactgtcg gctgtccaga
     1141 gcgccatagc caacgcgacg gatggcgacg tcgtggcagt gcccgcgggc gattgctcgt
     1201 ggggctcgct cacgatctcg aagggtattc acttgcgagg cgcgggcgca ggcgcaacga
     1261 acatcacgtt gagcggcgcg atcacgctga ggaagcacgc cacgcgcagc gtcgaagtca
     1321 gcggattcac gttcaggcgc agcggcggcg gcaattcggc gaaaatgatg tatgtcgaag
     1381 ggccatggaa cggcgagccg cccctcattc acgacaacgt gttcatcgcc agcaactccg
     1441 gaatcatccg ttaccagact aacggtggcg tgatctatcg aaacaccttc cgaggcgacc
     1501 tcgacgactc ggccctgcag cacaaggtgg acggcgacag tgaatcgtgg tccacgccgg
     1561 atacgatggg aacacgcgac accaacggta agcgcaacct gtacgtcgaa gacaacgtct
     1621 tcgagggcat ggcgaatcag gccacggact tcgacgacgc ggccagggtg gtgttccgtc
     1681 acaacacgct ggtcagctca tcgttcaaca gccacggtct cgacacctcg cccattggag
     1741 tgcggcatta cgagatctac gagaactcgt tccgctaccc caacaagtcc gtcaaccaaa
     1801 gctggcacat ctggctgcgt ggtggcaccg gagtgatctt caataaccag gtcgaggacc
     1861 tcaacggaac tcagtggggt aacaagacgg agctgctgtt ctccgtgcgc gccgcggtag
     1921 acggtggagt cgaaggttgc tgccgatcgt atccttgcaa gcatcaggtc ggccagaacc
     1981 acgacggcag cagccagttc accgacccgg ttcgcctctg gaacaactcc gggacgctcg
     2041 catggggcct caattcggcc tggaataaca cgtgcggtca gaacatggag gactacctgc
     2101 agaatggtcg cgacttcatc ttcgcaacct cgccgaagag cggctacacg ccttacccct
     2161 atccgcaccc gcttcgcgtc gacacggctc gtccgttgcc gccgtcgggc ctgaccgtgg
     2221 ctccctgaca cgcccgcccc tcgtccggcg agaggcgcta ccgcccgggc ggatcgagtc
     2281 cgcccggtgc gttcccctcc cttcggccga ccgcgaagcg cccgtcggcc gcgtcgtccc
     2341 ctcgcccccg ccgctgtcct cgcaggtcgc gcttctcgcc cgagacggac gattgcgtga
     2401 ctcgcgccac gacgaccgcg cggccattcg gctatccttt gggcgcccgg tgaggagcga
     2461 ccgtcgcgcc tcgggacaca caccggaagc cgacccggac cgcgcctcct cgagctgctc
     2521 tgcaagcgtc gcacacgtat tggg
//
LOCUS       DATMBB010000349         3465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_168942, whole genome shotgun sequence.
ACCESSION   DATMBB010000349 DATMBB010000000
VERSION     DATMBB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168942"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..647
                     /gene="aroH"
                     /locus_tag="VNL92_07510"
     CDS             246..647
                     /gene="aroH"
                     /locus_tag="VNL92_07510"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWO94595.1"
                     /translation="MVRCRGVRGATTVDENSREAIVEATVELLTAMVRENDLLADDVA
                     SAFFTTTPDINAEFPALAAREALKWTNVAMLCGHEMSVPGSLPKCLRILLHANTEKRQ
                     DEIVHVYLKGARVLRSDLAEGEREIAAPHSI"
     gene            686..1741
                     /gene="aroF"
                     /locus_tag="VNL92_07515"
     CDS             686..1741
                     /gene="aroF"
                     /locus_tag="VNL92_07515"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWO94596.1"
                     /translation="MIVIMKVGATDEDLNHVLERLQEVGLSGEVTHGVERNIVGVVGK
                     VFPELSTMIETLPGVDHTVPVSRPYKLASREFRPASTVIHVSGVAIGANETVVMAGPC
                     SVESEEQLLSTARAVKAAGAHILRGGAFKPRSSPYSFRGLGEEGLRYLAAAREETGMP
                     VITEVLTVKDVDLVAQYADILQIGARNMQNFILLEEVGRTGKPIMLKRALSGEIEEWL
                     LCAEYILAQGNRQVMLCERGIRTFERATRNTMDLSAVPLIKELSHLPIIVDPSHGTGK
                     RSLVGPMGVAGIAAGADGLMIEVHPNPDHALSDGAQSLTPDDFEHMMQSVGRVADAIG
                     RSLARPADAVHIGAAAG"
     gene            1738..2574
                     /locus_tag="VNL92_07520"
     CDS             1738..2574
                     /locus_tag="VNL92_07520"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWO94597.1"
                     /translation="MMPKRAAAPSARTLDSERGAAASRTLRLDNFEGGLDLLLHLIEK
                     QNLDITAISLARVTDQYLQILHSDTSVDAGALADFIAIGSKLLLIKSRALLPQPELSA
                     GGDQEDETAVDLTEALLEYRKFKEAAKLLRSREDSNLRAYPRAVPPPGVELPPGLDDV
                     TGETLLRLFQEALSREEQVRPTPLRPIERDTITLRDKVAQLRTAIRRSRMLSFRRLVA
                     GCQSRVEIVVQFMAVLELIKSGEVEARQAALFADIELVAAGHSSGQTSTPADAADREA
                     IA"
     gene            complement(2587..3117)
                     /locus_tag="VNL92_07525"
     CDS             complement(2587..3117)
                     /locus_tag="VNL92_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94598.1"
                     /translation="MATVAADAILGYATGRALRLSGRWTVYLILMANSPDFDYVPGLL
                     VGDPDSTHRNIGFHTPYFAAGIAVVATLAYRWRRPSEGWSRALWVGSLSGLMAIGHLV
                     LDQLELPYWHATGWMALPAEAVNSVVDTLVIGPVIVTAFWIWHVRFAGAANGGGRTSN
                     TRGGTGASGSERYKAD"
     gene            complement(3127..>3465)
                     /locus_tag="VNL92_07530"
     CDS             complement(3127..>3465)
                     /locus_tag="VNL92_07530"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWO94599.1"
                     /translation="PIPLYWNGEQVRDFIYVEDLARAHVVPLGLGGLNVYNVGTGRGV
                     RVRHVVETIFAILGYETPIEDLGPRPGDVDAYWASPEKITAELGWRAEIDLAEGLRRT
                     VDFFRSRLGG"
BASE COUNT          651 a         1070 c         1153 g          591 t
ORIGIN      
        1 gttgggccga tgccgccgcc atcgggagtg cgatcgtccg gctgctgcaa gaagcagcgc
       61 cgggggagcg cgaggagaag gttcgacgct atgtggagtt ggtcgccggc cgttcgagcg
      121 ccgatgtccc gcgctgaggc atgctctcag gcgagtcacg tcaccgggac tttcgagctc
      181 gcggccggta tgtttgtgcc catcctcgtg taagcgccat catgggaagg tgaagcgagt
      241 tgggcatggt ccgatgtagg ggtgttcgcg gggcaaccac ggtcgacgag aacagccgcg
      301 aggcgatcgt cgaagcgact gtcgagttgc tgacggccat ggtgcgggag aacgatctgc
      361 tcgccgatga tgttgcgagc gccttcttca cgaccacgcc cgacatcaac gcggagttcc
      421 cggcacttgc ggcgcgcgag gccctgaagt ggacgaacgt ggcgatgctc tgcggccacg
      481 agatgtcagt tccgggcagc ctacccaagt gcctgcgtat tctcctccac gcgaatacgg
      541 aaaagaggca ggacgagatc gtccacgtct atctgaaggg cgcgagggtg ctccgctccg
      601 acctcgcgga aggggagcga gagatcgccg ccccgcactc gatatgaaga cagattagac
      661 ccagctggga gaaggtgcga agacaatgat tgtgatcatg aaggtgggcg caaccgacga
      721 ggacctcaac cacgtcctcg agcggctcca agaagtcggc ctttcgggcg aggtgacgca
      781 cggggtcgaa cgcaacatcg taggcgtcgt cgggaaagtg ttccccgagc tgtcgacgat
      841 gatcgaaacg ctgccgggag tggaccacac cgtacccgtc agccgcccgt acaaactggc
      901 gtctcgcgag ttcagaccgg caagcacggt catccatgtt tccggcgtcg cgattggggc
      961 gaacgaaacg gtcgttatgg ccggcccctg ctcggtggag agcgaggaac agctgctatc
     1021 gaccgcccgt gcggtaaagg cggcgggagc acacattctg cgaggtggcg ccttcaagcc
     1081 gcgcagctcg ccgtacagct tccgtggcct gggcgaggaa ggccttcggt atctggcagc
     1141 cgcccgggag gaaaccggta tgccagtcat caccgaagtt ctcacggtga aggatgtcga
     1201 tctcgtggcg cagtacgctg acatcctcca gatcggtgcg cgcaacatgc agaacttcat
     1261 cctgctggag gaggtgggcc gtacggggaa gcccattatg ctgaagcgtg cgctatcggg
     1321 cgaaatcgag gaatggctgc tgtgcgccga atacatcctt gcgcagggca atcgccaggt
     1381 catgctgtgc gagcgcggca tccgcacttt cgagcgggcg acgcgcaata cgatggattt
     1441 gagcgcagtt cctctcatca aggagctgag ccatcttccc atcattgtcg acccgagcca
     1501 tggcacgggg aagcgcagcc ttgtgggccc gatgggcgta gcgggcatcg cggccggcgc
     1561 ggatgggctc atgatcgagg ttcacccgaa tcccgaccat gcattgtccg atggtgcgca
     1621 gtcgcttacg cctgatgact tcgaacacat gatgcagagc gttggtcgtg tcgccgatgc
     1681 gattggacgg tcgcttgccc gccctgcgga cgccgttcat atcggcgcgg cggctggatg
     1741 atgccgaagc gtgccgccgc cccgtcggct cgaacgctcg attcggaacg aggggcggcg
     1801 gcctcgcgta cactgcggct cgacaacttc gagggaggcc tcgaccttct tctgcatctc
     1861 atcgagaagc agaacctcga cattaccgcg atctcgttgg cacgggtcac cgatcagtat
     1921 ctgcagatcc tccattcgga cacgagtgtc gatgccgggg cgctggcaga tttcattgcc
     1981 atcggcagca agcttctgct cataaagtcg cgagccttgc tgccgcagcc ggagctgagc
     2041 gccggaggcg accaggagga tgagacggcg gtcgatctga ctgaagcgct gctcgagtat
     2101 cggaagttca aggaggcggc caagctgcta cggagccggg aggacagcaa ccttcgcgcc
     2161 tatccgcgtg ctgttccccc tcccggcgtg gagctcccac ccggactcga cgacgtgacg
     2221 ggggagacgc tgcttcgact gttccaggaa gcgctctccc gggaggagca ggtgcgaccg
     2281 acgccgctcc ggcccatcga gcgcgatacc atcacgctgc gcgacaaggt ggcccaactg
     2341 cgaaccgcta ttcgccgatc gcgcatgttg agcttccgtc gactcgtggc gggctgccag
     2401 agcagggtcg agatcgtggt tcagttcatg gccgtgttgg agctcatcaa gtcgggcgag
     2461 gtggaagctc ggcaggcggc gctgttcgcc gatatcgagc tcgtggctgc tgggcattcg
     2521 tcgggccaga cttcaacgcc ggcggacgcc gccgaccggg aagcgatcgc gtaagccgat
     2581 cgccggtcaa tcagccttgt atcgctccga accggaagca ccagtgccgc cccgtgtatt
     2641 cgaggtacgc ccacccccgt ttgccgcgcc ggcgaacctc acgtgccaga tccagaacgc
     2701 cgtcacgatc accggcccga tgacgagagt atcgacgacg gaattcaccg cctccgccgg
     2761 aagggccatc catcccgtgg cgtgccagta gggtaactcg agttggtcaa gcacgaggtg
     2821 cccgatcgcc atgaggccgg acaaggagcc cacccaaagg gctcggctcc agccctcgct
     2881 cggacgtcgc catcggtagg ccaaagtggc aacaactgcg attccggccg caaagtacgg
     2941 cgtgtggaag ccgatgttcc ggtgggtcga gtccgggtcg cccacgagga ggcccggcac
     3001 gtaatcgaag tcgggcgagt tcgccataag gatgaggtac acggtccatc gacccgatag
     3061 gcgcagcgcc cgtccggtcg cgtagccgag gattgcatcc gctgcaacgg ttgccatcga
     3121 ggacagctag ccaccgaggc gcgagcggaa gaagtcgacc gtacggcgca ggccttcggc
     3181 gagatcgatt tccgccctcc accccagctc agccgtgatc ttctccggcg atgcccagta
     3241 ggcgtcgacg tcgcccggtc gcggccccag gtcctcgatc ggggtctcgt agccgaggat
     3301 cgcgaagatc gtctcgacga catgtcgcac gcgaacgcct cgccccgtgc cgacattgta
     3361 gacgttcagg ccccctaggc cgagcggaac gacgtgggcg cgcgccaagt cctcgacgta
     3421 gatgaagtcg cgcacctgct cgccgttcca gtacagcggt atcgg
//
LOCUS       DATMBB010000350         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_457101, whole genome shotgun sequence.
ACCESSION   DATMBB010000350 DATMBB010000000
VERSION     DATMBB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457101"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VNL92_07535"
     CDS             <1..438
                     /locus_tag="VNL92_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWO94600.1"
                     /translation="VVPFAGHQATLAGLREALAGKVVVDAVVPMEFRDGSPRAIWVEE
                     GSAAEQAQALLPQSSVVGAFHHLSARKLRSAAALDADCLVAGDDDAAKAIVFDLVREI
                     AALRPLDAGGLSGAYQVELFTAALLNINRRYKTQASVRIVGVE"
     gene            446..1198
                     /gene="cofE"
                     /locus_tag="VNL92_07540"
     CDS             446..1198
                     /gene="cofE"
                     /locus_tag="VNL92_07540"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HWO94601.1"
                     /translation="MAELRAWGLKGIPEVQPGDDLAALIIEALRSGDHSLAPGDVLVV
                     TQKIVSKAEGRLVDLDTVEPSHFARQLAEAWGKDPAMVEVVLRESRRIVRMDNGVIVA
                     ETRHGFVCANAGVDASNVAGDRTVALLPLDPDASAARLRAAIGRAMSVAPAVVISDSF
                     GRPWREGTTEVAIGVTGMPAARDYIGVRDAFGYELRTSVVAVADELACVAELVCGKIE
                     GTPVALIRGYDGGEGEGKGQDLVRAPERDLFR"
     gene            1244..1585
                     /locus_tag="VNL92_07545"
     CDS             1244..1585
                     /locus_tag="VNL92_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWO94602.1"
                     /translation="MNECLFCRMADGQMDVAKLHDDSLVFAIRDINPRAPVHIMVIPK
                     AHIPTARDLSEADGQLLARMFTVANLMADKEGIGERGYRLAINCGDDAGMSISHLHLH
                     LLGGRRLGAEG"
     gene            1585..>2116
                     /gene="yqeK"
                     /locus_tag="VNL92_07550"
     CDS             1585..>2116
                     /gene="yqeK"
                     /locus_tag="VNL92_07550"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HWO94603.1"
                     /translation="MRAEDLRRRMGHLPDGLLRHIDRVVGEALVLAEAHGLDADHVAL
                     AAQGHDLARATPPDQLLRLALELGLNVSDVDRADPILLHGPVGAELLAREYGIEEPGV
                     LRAARYHTTSYPGLGSLESVVFLADKLEPDKLRRDDALRPVRERALADLDDGVRAMVD
                     LLTKRAVERGWALHPWL"
BASE COUNT          319 a          729 c          752 g          316 t
ORIGIN      
        1 gtcgtgccct tcgcagggca ccaggccacg ctggccgggc tccgggaggc gctcgccggc
       61 aaggtcgtgg tcgatgccgt cgttccgatg gagtttcgtg atgggagccc ccgcgcgatc
      121 tgggtggaag aaggctcggc cgccgagcag gcgcaggccc ttctgccgca gagctccgtg
      181 gtcggcgcct tccaccacct gagcgcgcgc aagctgcgct cggcagccgc gctcgatgcc
      241 gactgtctcg ttgccggcga cgacgacgcg gcgaaggcca tcgtgttcga tctggtccgc
      301 gaaatcgcgg cgctccgtcc actcgacgcg ggcggcctga gcggcgcgta ccaggtcgag
      361 ctctttacgg cggcgcttct caacatcaac cgacgctaca agacgcaggc aagcgtgcgc
      421 atcgtcgggg tggagtgagc gagacgtggc cgagctgcgg gcgtgggggc tgaagggcat
      481 cccagaggtg cagccgggcg atgacctcgc cgcgctcatc atcgaggccc tccgttctgg
      541 cgaccactcg ctcgctcccg gcgatgtcct ggtcgtcacg cagaagatcg tgtccaaggc
      601 cgagggacgc ctcgtcgatc tcgacacggt ggagccgtcc cacttcgcgc gccagctcgc
      661 ggaagcctgg ggcaaggacc cggcgatggt ggaggtggtg ctccgcgaga gccggcgcat
      721 cgtccggatg gataacggcg tcatcgtcgc cgaaacacgc cacggattcg tctgcgcgaa
      781 cgcgggcgtc gacgcatcga acgtggccgg cgaccggacc gtcgccctgc tcccgctcga
      841 ccccgatgcc tcggccgcgc ggttgcgggc ggccatcggt cgagcgatga gcgtagcccc
      901 ggccgtcgtc atctccgata gcttcggccg gccgtggcgc gaggggacca ccgaggtggc
      961 catcggcgtc acgggcatgc ccgcggcgcg cgactacatc ggcgtgcgcg acgccttcgg
     1021 atacgagttg cgcaccagcg tggtcgccgt tgccgacgaa ctcgcctgcg tggcggagct
     1081 cgtatgcggc aagatcgagg gcacaccggt cgcgctcata cggggctacg atggcggcga
     1141 gggcgagggg aaaggccaag acctggtacg cgccccggag cgcgacctgt tccgctgagc
     1201 cgggcgctgg gcccgtgagg acgctggacg aggaggcgtg aacatgaacg aatgtctctt
     1261 ctgccggatg gcggatggcc agatggatgt ggcgaagctc cacgacgatt cgctcgtctt
     1321 cgccattcgc gatatcaacc cgagggcgcc ggtccacatc atggtcatcc cgaaggccca
     1381 catcccgacc gcacgagacc tcagcgaagc cgacgggcag ctgctggcga ggatgttcac
     1441 ggtcgcgaac ctcatggccg acaaggaggg catcggcgag cgcggctacc ggctggcgat
     1501 caactgcggt gacgacgcgg gcatgagcat ctcccatctc cacctccacc tgctcggcgg
     1561 acggcgcctg ggggcggagg ggtagtgcgc gccgaggacc tgcgccgtcg catgggccac
     1621 ctgcccgatg gcctgctgcg ccacatcgac cgtgtcgtcg gcgaggcgct cgttctcgcg
     1681 gaggcccacg gactcgatgc agaccacgtg gccctcgcgg ctcagggcca cgacctggcc
     1741 cgcgccacgc cgcccgacca gctgctgcga ctcgcgctcg agctcgggct caacgtgagc
     1801 gatgtcgacc gggcggaccc gatcctgctg catgggccgg tcggggccga gctcctcgcc
     1861 cgggaatacg gcatcgaaga gcccggcgtg ctgcgcgccg cgcgctacca cacaacgtcg
     1921 taccccggcc tcggctcgct cgaatccgtc gtgttcctcg ccgacaagct ggagccggac
     1981 aagcttcggc gcgacgatgc ccttcgaccg gtgcgcgagc gtgcgctcgc ggatctcgat
     2041 gatggcgtcc gcgccatggt cgacctcctc accaagcgtg ccgtcgagcg aggctgggcc
     2101 ttgcacccgt ggctgg
//
LOCUS       DATMBB010000351         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_64676, whole genome shotgun sequence.
ACCESSION   DATMBB010000351 DATMBB010000000
VERSION     DATMBB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64676"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VNL92_07555"
     CDS             complement(<1..373)
                     /locus_tag="VNL92_07555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWO94604.1"
                     /translation="MIEEAGVVIAFWVLAVTTIVNAVMVAAVRNLLHAVLFLVLTFVG
                     LAGLFITLSADFVAAVQLLVYAGAVAVLMVFAIMLTPRAARDNADNEFSLPALVLSGL
                     MAAVATFVALDAGWRTQTPGDV"
     gene            complement(412..1674)
                     /gene="nuoH"
                     /locus_tag="VNL92_07560"
     CDS             complement(412..1674)
                     /gene="nuoH"
                     /locus_tag="VNL92_07560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWO94605.1"
                     /translation="MTLLGQWYDIRDLSELVAGLTGWLRDTFDAGDGVIYVVSALLGA
                     VGILSFIGATAIVNIWIERRLVGRMQNRLGPNRVGPFGLLQPVADAIKLIQKEALMPR
                     VADPIVFTLAPILVFVPAILAWAVLPWGERMTLASLDAGVLYFVALGTVAVLAVFMAG
                     WSSNNKFSLLGAMRVVPMLLSYEIPIVLAFLGPVLLAGTMNLSGIARWQADYHVWIIF
                     LQPLTFLIYFIAASAELNRTPADIAEAESEILAGYHTEYSGMKFGLFYAVELVNALLV
                     SGLAASMWYGGWYLFEIDRVLPGWLVFIGKMYALYFLLIWTRGTLPRLRIDQLVALSW
                     KFMTPLALANVVLVSAEVVIWAETGVSAGVILPLSAVVNVTLTGVLLVAWLRFMLGKV
                     ERLPRRPRLYREVVVPPIAAPEQAAARP"
     gene            complement(1671..>2375)
                     /locus_tag="VNL92_07565"
     CDS             complement(1671..>2375)
                     /locus_tag="VNL92_07565"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWO94606.1"
                     /translation="REREKILDLFEMTCGARLTTNYMRIGGVAFDIPDEFLPRLRALI
                     DELPERIAEYEELLLDNEILLLRGRDVGVITKELAVNASLSGPMLRSTGIAWDVRKGD
                     PYCGYETYDFEIPVGHNGDAYDRFVVRMEEMKQSVRIIEQAMERLPDGPYKADVPLAI
                     RPPAGEVYSRIESPKGELGFYLVSDGGSAPWRFHVRSPSLINLSALREMVVGLGIADA
                     IVAFGSIDINVGELDR"
BASE COUNT          442 a          779 c          821 g          333 t
ORIGIN      
        1 cgacgtcgcc cggggtctgc gtgcgccagc cggcgtcgag tgcgacgaac gtggcgaccg
       61 cagccatgag tcccgagagc accagcgccg gcaggctgaa ctcgttgtcg gcgttgtcgc
      121 gcgcggcgcg gggggtcagc atgatggcga agaccatcag caccgccacg gcgccggcgt
      181 agaccagcag ttgtaccgcc gccacgaaat cggccgagag cgtgatgaac agcccggcga
      241 gcccgacgaa cgtgaggacg aggaagagca ccgcatgcag caggttccgc accgcggcga
      301 ccatgacggc gttgacgatg gtggtcaccg ccagcaccca gaaggcgatg accactccgg
      361 cttcttcgat catcgtcggt cgcttcccgt ctcctgagcc cggcgcccgc ttcagggccg
      421 ggcggccgcc tgctcgggcg cggcgatcgg cggcaccacg acctcgcgat agagccgcgg
      481 gcgccgcggc agccgctcca ccttgccgag catgaaccga agccacgcca cgagcaagac
      541 gcccgtgagc gtgacgttga cgaccgccga aagcgggagg ataacgcccg cgctcacgcc
      601 cgtctccgcc cagatgacga cttcagcgga gaccagcacg acgttcgcca gcgcgagggg
      661 cgtcatgaac ttccacgaca gcgccacgag ctggtcgatg cgcagccgcg gcagcgtacc
      721 gcgcgtccag atgagcagga agtacagggc gtacatcttg ccgatgaaga cgagccagcc
      781 cggcaggacg cggtcgatct cgaacaggta ccacccgccg taccacatcg acgccgcgag
      841 gccagagacc aggagggcgt tcaccagctc gacggcgtag aagagcccga acttcatgcc
      901 ggagtactcg gtgtggtacc cggcgaggat ctccgattcg gcctcggcga tgtccgcagg
      961 cgtgcggttc aactcggccg acgcggcgat gaagtaaatc aggaaggtca gaggttgcag
     1021 gaagatgatc cagacgtggt agtcggcctg ccagcgagcg atgcccgaca ggttcatggt
     1081 ccccgccagc agcaccggtc ccaggaacgc gaggacgatg ggaatctcgt agctcaggag
     1141 catcgggacc acgcgcatgg ctccgagcag ggagaacttg ttgttcgatg accagccggc
     1201 catgaagacg gcgagcacgg cgaccgtgcc caacgcgacg aagtacagca cgccggcgtc
     1261 gaggctcgcg agggtcatgc gctcgcccca gggaagcacc gcccacgcga ggatggccgg
     1321 cacgaacacg agaatcggcg cgagggtgaa cacgatgggg tcggccaccc gcggcatcag
     1381 cgcttccttc tgaatgagct tgatggcgtc ggcgaccggc tgaagcagcc cgaagggacc
     1441 gacccggttg ggccccaggc ggttctgcat gcggccgacc agcctgcgct cgatccagat
     1501 gttcacgatg gcggtggccc cgatgaacga caggatgccg acggcgccca gcagcgcgga
     1561 gacgacgtag attacgccgt cgccggcgtc gaacgtgtcg cgcaaccagc ccgtgagccc
     1621 ggcgacaagc tcggaaaggt cgcggatgtc gtaccactgg cccagcagcg tcaccggtcg
     1681 agctctccca cgttgatgtc gatgcttccg aacgcgacga tggcgtcggc gatgccgagg
     1741 ccgaccacca tctctcggag ggcgctgagg ttgatgagcg atggtgagcg cacgtggaac
     1801 cgccagggcg cggagccccc gtcgctcacc aggtagaagc cgagctcgcc cttcggggac
     1861 tcgatgcggc tgtacacctc gccggcgggc gggcggatgg ccagcggcac gtcggccttg
     1921 tacggcccat cgggaaggcg ctccatcgcc tgctcgatga tgcgcacgct ctgcttcatc
     1981 tcttccatgc gcacgacgaa ccggtcgtag gcgtcgccgt tgtggcccac gggtatctcg
     2041 aaatcgtacg tctcgtatcc gcagtacggg tcgcccttgc gcacgtccca ggcgatgccc
     2101 gtcgagcgca gcatgggccc gctgagggag gcgttgacgg cgagctcctt cgtgatgacg
     2161 cccacatcgc ggccacggag gaggaggatc tcgttatcga gcaggagctc ctcgtactcc
     2221 gcgatgcgct ctgggagctc gtcgatgagc gcgcgcaggc ggggaaggaa ctcgtccggg
     2281 atgtcgaacg cgacgccgcc gatgcgcatg tagttcgtgg tgaggcgggc gccacaggtc
     2341 atctcgaaca ggtcgaggat cttctcgcgc tcgcg
//
LOCUS       DATMBB010000352         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_255545, whole genome shotgun sequence.
ACCESSION   DATMBB010000352 DATMBB010000000
VERSION     DATMBB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255545"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(526..2322)
                     /locus_tag="VNL92_07570"
     CDS             complement(526..2322)
                     /locus_tag="VNL92_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94607.1"
                     /translation="MEEHNYWTTVFHRRLSRRRVLVATGSAGGAALTAGVLGCGDDDS
                     DGSGAADEMRGTAVAETDGVKTGGTYRAPIVMPTGIDVMKNPGGAAQVLGAFSLSRLL
                     KYETGFDDPATEDLDFARERWYNPVPDLAASWEIPDGQTFIFTLQPDARWHDIDPTNG
                     RAVTAEDIVFAYGYYQSARPDGGALLSRVVEGVDAVAPDKVQFRLNQAFAPFLGYMSR
                     AQDLWIYPPELIEADGDGSKRIVGSGPWLFESLQRDVGVRYRRNPNYWVKDDQGRSLP
                     YMEVFEGADVPDPNTQITQFQSGNILQVSVPAALLSQFRSQNSDAVIRGGFANLASFL
                     FFPPGTYDENLPPFNDVRVRRAVSHAIDRDALIALGSNDAGGQWNNLINAGAQWYLDP
                     AGPEMGPAAEFYEYNPDRAKELLADAGYESLDLDLYFNRNGYVSPFPFYNPIAEALPA
                     MMEASNINLTLKGVDYLSEWINPESGIFWGKLPPNSIAWALNIIDGDPDGYLHGMLHP
                     DGTHNNPGHSRVRIDEFTRLVEEQRRELDVDTRRELVFELQRRVSDQMIFVPMVAIRQ
                     FQAWQPFVKNRFGGGTSYGFPTEDLIYYWLDR"
     gene            complement(2368..>2938)
                     /locus_tag="VNL92_07575"
     CDS             complement(2368..>2938)
                     /locus_tag="VNL92_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94608.1"
                     /translation="IATVIGVVSGFYGRWLDTLAQRVVDIWMAFPALVLLITIISIFS
                     STGRLQLVIALGIILAAGTSRIVRGATLALKANMYVEAARSIGASDLRVMVVHIIPNL
                     VPIIIVLATVQIGAAILAEATISFLGYGIAPPFPTWGQMLSPSELALMRQHPHLSLFP
                     GLTIAVTVYAFNVLGDALRDILDPRLRGT"
BASE COUNT          555 a          919 c          900 g          564 t
ORIGIN      
        1 cacccgacga cttcgcgcac gcggaggtct acgcgacctt ggcaaccatt cgcgccatag
       61 tcggcgaggc ctttctcttc tcagtggact tcagcgcggg ccgaccggag ctcatcacct
      121 acaacaagag cgacttcgcc gatgtgtgaa gacgcagccg gattacctgt cggagcagca
      181 gttgggtgga tgggcggccg gctcgatcgg cacgtcgaca tccgccgctg cacatcgctg
      241 aaggcgcctc gctcgccgca gatccaccgc aggtttgccg cgagacagct caagctgcta
      301 actcataagc ccttggtcgg gggttcgaat ccctcccctg ctaccagcat cccgaatctc
      361 gtcagggccc ttaacggagg catactccgc tagggctctt cgcaatcgtg ggctttccgg
      421 gcagagcctg acagaaggcg ggtggcctcc tgcccgcact cacccgggcc ctcggacccg
      481 ccgcatcgag cgtgcccctg gccctcgcct catgatcgac ccaggttacc ggtccagcca
      541 gtagtagatg aggtcctcgg tcggaaagcc gtacgacgtt cctcctccga agcggttctt
      601 cacgaatggc tgccaggcct ggaactggcg gatagcgacc atcggaacga agatcatctg
      661 gtccgatacg cgccgctgga gttcgaacac tagctcgcgc cgcgtgtcga catcgagttc
      721 gcggcgctgc tcctcgacca gccgggtgaa ctcgtcgatc cggacccggc tgtgaccggg
      781 attgttgtga gtaccgtcgg gatggagcat cccgtgcagg tagccgtccg gatcgccgtc
      841 gatgatgttc agtgcccatg cgatgctatt gggcgggagc ttgccccaga agatccccga
      901 ctcagggttg atccattccg aaaggtagtc gacccccttg agcgtgaggt tgatgttcga
      961 agcctccatc attgcgggaa gcgcctcggc gatgggattg tagaacggga acggcgagac
     1021 gtagccgttg cggttgaaat acaagtcaag atcgagcgat tcgtatcccg cgtcggcaag
     1081 cagctccttc gcccggtctg ggttgtattc gtaaaactca gccgcgggtc ccatctccgg
     1141 tccagccgga tcgaggtacc actgggcacc cgcgttgatg aggttgttcc actgtccgcc
     1201 agcgtcattc gagccgaggg caatgagcgc gtcacggtct atcgcgtgtg agacggcgcg
     1261 ccgaactcgg acatcgttga acggcggtag attctcgtca tacgtccccg gagggaagaa
     1321 taggaagctg gcaagatttg cgaaaccgcc gcgaatgacc gcatccgaat tctgtgaccg
     1381 gaactgactg agtagggccg ctggcacact gacctgcaag atgttgcccg attggaactg
     1441 agtgatctgt gtattcgggt ccggtacatc ggcaccctcg aagacttcca tgtatgggag
     1501 tgaccgtccc tggtcgtcct tcacccaata gttgggattg cggcgatacc gaacgcctac
     1561 gtcgcgctgc agtgactcga agagccaggg tccgctcccc acgatgcgct tgctgccatc
     1621 gccgtccgcc tcgatgagtt cgggcgggta aatccacaga tcctgtgcgc ggctcatata
     1681 gccaaggaag ggcgcaaacg cttggttgag gcggaattgc accttgtcgg gtgctaccgc
     1741 atcgactcct tcgacgacgc gactgagcaa tgcgccacca tcgggtcgag cgctctggta
     1801 gtagccgtag gcgaagacga tatcctctgc cgtcaccgcc cgaccgttgg tcgggtcgat
     1861 gtcgtgccat cgagcgtccg gctgaagtgt gaagatgaac gtctggccgt ccggtatttc
     1921 ccagctcgcc gcgaggtctg ggacggggtt gtaccagcgc tcacgcgcga aatccaaatc
     1981 ctccgtcgcc gggtcgtcga agcccgtctc atacttcagc agtcggctca ggctgaaggc
     2041 accgagtacc tgggcggcac cgccggggtt cttcatgacg tcgatgcccg tcggcatgac
     2101 gatcggagcc cgatacgtgc cacctgtttt cactccgtct gtctctgcca cggccgtgcc
     2161 acgcatctca tcggctgccc ccgacccgtc ggagtcgtcg tccccacacc cgaggacgcc
     2221 ggcggtgagt gcagcacctc ccgcggaccc ggtcgcgacg aggacgcgtc gccgcgatag
     2281 tctcctgtgg aatacagtcg tccagtagtt atgctcttcc attcgcgtgc cttccccctt
     2341 cttcgcttgg agcccggctc cttacctcta cgttccgcgc agtcgtgggt ccagaatgtc
     2401 ccggagcgcg tctccgagga cattgaaggc gtaaacggtg acggcgatag tgagccccgg
     2461 gaagagcgat aggtgcggat gctgccgcat caacgcgagt tcggatggac tcagcatctg
     2521 accccacgtt gggaatggag gcgcgatacc gtagccgagg aaggagatcg tcgcctcggc
     2581 gaggatggcc gcaccaatct gtacggtggc cagcacgatg atgatcggca cgaggttcgg
     2641 gatgatgtgt acaaccatca cgcgcagatc gcttgcgccg atggatcgcg cagcctccac
     2701 gtacatgttc gccttcaagg caagcgtggc gccgcgaaca atgcgcgacg tacctgctgc
     2761 caggatgatc ccgagcgcta tcacgagctg aaggcgtccc gtcgaactga agatactgat
     2821 aatcgtgatc aacagcacaa gggcggggaa cgccatccaa atgtcgacca cgcgctgagc
     2881 gagcgtgtcg agccagcgac catagaagcc gctcaccacg ccgatgaccg tcgctatc
//
LOCUS       DATMBB010000353         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_487055, whole genome shotgun sequence.
ACCESSION   DATMBB010000353 DATMBB010000000
VERSION     DATMBB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487055"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VNL92_07580"
     CDS             complement(<1..1262)
                     /locus_tag="VNL92_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94609.1"
                     /translation="MQSEYWRAFQRRATRRRVLRGTAIGAAGIAGTVTLGACGDDDDD
                     DEPSNGGEASPAATSTGGGAAEGARPGGRFQYGSNVEFDTFDPHLSIAASTAFFPKVY
                     NLVAHQSAVDPSFMLFDLSESVEQIDDLTYTFKIRPGVKIAPNDLGAPERDLDANDAL
                     VSFQRIQAEPRANAGAFVKQRVASFEAPDPTTFTVRTNEPYAWFFNGIGSFVATVVPR
                     ELIEGDLGQLRTRGAGGGAYRLTNFTEGEGATLERNPNYYRKPLPYIDGYDIKVIPDR
                     AARRVAFLDRQTYFYQAENVAEAEDLQNQGDFVLVQEPYFTFYSFTMNAERDPWQDAR
                     IRRAAHRAINRQQFADVLQPGNGRPNGLVHWPTGPYALSEDELAELQPHDPDEARRLL
                     EEAGYPDGITIRLMYPSGLEIPNYLPIL"
     gene            complement(1465..>2122)
                     /locus_tag="VNL92_07585"
     CDS             complement(1465..>2122)
                     /locus_tag="VNL92_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWO94610.1"
                     /translation="HARARVGYGRGVGGRTVALEMEPQAKPDKAGFFDRQQLAEQFKG
                     TARAEHGALVICGQWMAEETDITAKMKLARQTWDEGRHAMAFYKRARELGADIGLEPP
                     TSAVLAPLGPMLVLARDTVERTTVLNRCIEALAIEGFSNTKPLADPESAEMLDFVIAD
                     EVSHVQLGQWAVQHFCKTPEDLARVAEFQGRCEELLAQGRQEAARELEQQALTGCSPY
                     "
BASE COUNT          326 a          666 c          720 g          410 t
ORIGIN      
        1 aagaggatcg ggaggtagtt cgggatctcc agtccactcg gatacatcag gcgaatcgtg
       61 atgccgtcgg gatagcccgc ttcctccagc agccgacggg cctcgtccgg atcgtgtggc
      121 tggagttcgg cgagttcgtc ctccgagagg gcgtagggac cggtcggcca gtggacgagg
      181 ccgttcggcc gcccgttccc cggctgtaat acatcggcga actgctgccg gttgatggcc
      241 cgatgggccg ctcgccggat ccgcgcatcc tgccagggat cgcgttcggc attcatcgtg
      301 aaactgtaga acgtgaagta cggctcctga accaggacga agtcaccctg attctggagg
      361 tcctcggcct cggcgacgtt ttcggcctgg tagaagtagg tctggcggtc gaggaaggca
      421 acgcgacgtg cagcccggtc ggggatgacc ttgatgtcgt agccgtcgat gtacgggagc
      481 ggtttgcggt agtagttcgg gttacgctcg agcgtggcgc cctcgccctc cgtgaagttc
      541 gtgaggcgat atgcgccgcc tccggcgcct cgcgttcgca gttggccgag gtcgccctcg
      601 atgagctccc gcgggacgac cgtcgccaca aagctgccga tgccgttgaa gaaccacgcg
      661 tacggctcgt tcgttcggac ggtgaaggtg gttgggtcgg gggcctcgaa ggaggcgacc
      721 cgttgcttca cgaaggcgcc cgcattggcc cgaggctcgg cctggatgcg ctggaagctg
      781 acgagcgcgt cgttcgcatc gagatcgcgt tcgggcgcgc cgaggtcgtt cggcgctatc
      841 ttgactcccg gccggatctt gaaggtgtag gtgaggtcgt cgatctgctc cactgactcg
      901 ctgagatcga agagcatgaa actcgggtcg accgccgatt gatgcgcgac gaggttgtac
      961 accttcggga agaacgccgt cgacgccgct atggagaggt gggggtcgaa cgtgtcgaac
     1021 tcgacattgg agccgtattg gaaacggccg ccgggtctgg cgccctcagc agctccgccg
     1081 cctgttgaag tggccgccgg ggacgcttcg ccgccattgc tcggctcgtc gtcatcgtcg
     1141 tcatcgccgc aggcgccgag cgtgaccgtc ccggcgatgc cggcagcgcc gattgccgtt
     1201 cctcggagga cgcggcgcct cgtggcgcgt cgctgaaacg ctcgccaata ctcgctttgc
     1261 atgccgatct ccccctctcg aaatcatgcg tgcgtaggcg aaccaggcag tcgagcgtgt
     1321 gccttcggca ctcgctagtg cggcgtatga tgcgagaatg ccgcctggct gtcaagaaaa
     1381 cgcaagcagg cccggaaaag atctctcctt ccgggcccga atggcgttct ggcttcgatt
     1441 gtcgcgattg gaatctgcga ttcgctagta gggggagcag cccgtgagcg cctgctgctc
     1501 gagctcgcgg gctgcctcct ggcgcccctg ggccagcagt tcctcgcagc ggccctggaa
     1561 ctcggcgacg cgagcgagat cttccggcgt cttgcagaag tgctgcacgg cccactgccc
     1621 gagctggacg tggctcacct cgtcggcgat gacgaagtcg agcatctccg cactctcggg
     1681 atcggccagc ggcttcgtgt tcgagaagcc ctcgatggct agcgcttcga tgcagcgatt
     1741 cagtacggtg gtccgctcga cggtgtcccg ggcgagaacg agcatcggtc ccagaggggc
     1801 gagcacggcc gatgtcggtg gctccaggcc tatgtcggcg cccagctccc gcgcccgctt
     1861 gtagaaggcc atcgcgtggc gcccttcatc ccaggtttgc cgggccagct tcatcttcgc
     1921 ggtgatgtcg gtctcctcag ccatccactg accgcagatg acgagggcgc cgtgctcggc
     1981 ccgggccgtg cccttgaact gctccgcgag ctgttgacgg tcgaagaagc cagccttatc
     2041 gggcttggct tgcggttcca tttcgagagc cacggttctt cctcccacgc cgcggccgta
     2101 gccgactctc gcccgtgcgt gc
//
LOCUS       DATMBB010000354         2897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_334571, whole genome shotgun sequence.
ACCESSION   DATMBB010000354 DATMBB010000000
VERSION     DATMBB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334571"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VNL92_07590"
     CDS             complement(<1..186)
                     /locus_tag="VNL92_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWO94611.1"
                     /translation="MANTSAVTPAERTGSAVARAAKALRRFSFRSPLHLAVIALAVLW
                     SIPTIALLISSFRDPADV"
     gene            complement(186..1115)
                     /locus_tag="VNL92_07595"
     CDS             complement(186..1115)
                     /locus_tag="VNL92_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWO94612.1"
                     /translation="MIGRRWWAPWIWLAPAFILLAVFLLYPTIDTIRRSFLDARSEGW
                     VGLDNYRFIIENPNPIVADTHAALLHTVLWLALFTAVTVILGLVLAELTGRVRYESVA
                     KAAIFIPMAISFVAASVIWKFMYEFDPDIGTVNAVVSGLGGDPTAWLQNTNAPYAWFT
                     SLGPETLPGPLQINTFALITVGVWMWTGFAMVVLSAGLKGISTEVLEAARVDGANEWQ
                     VFTRITLPILSPTIVVVATTLVIQALKNFDLIWVMTGGRFQTDVVATLFFKEAFVLRD
                     FGVGAALAIVLMLFVVPLMLISIRRFQFQQETS"
     gene            complement(1148..2530)
                     /locus_tag="VNL92_07600"
     CDS             complement(1148..2530)
                     /locus_tag="VNL92_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94613.1"
                     /translation="MTSINTVVKQGGFMLKTRLMLLILVAVLSAGLAVACGDDDDDDE
                     EETTTPSGTTPAGAGEDLGGESVDVLGIWGDEELASFEAMVAPWQDDTGGSVEFTGTR
                     DITSLLTTRVEGGNPPDIAIPAEIGLFRQFAAEGELVPLSSCMGLEEQIMQNYPQSFI
                     ELGTVDGELYGFFMKADTKGTIWYDPQFFEENGYEPLSEDSTFDDLVALSDEIAAGDH
                     GVPPWSIGNEADAGSGFPGSDWIQQILLNEAGEQTYDGVVDGSVPFTDPAVRDAFEKF
                     GQIALTEGYVAQGGAEGINATNFQDATLLPFGDPPEAAMVYLGGFAAGFITDQFPDAV
                     AGEDYDFFPFPGGAVTGGANIVYAFNSDPATCSVLSWLAGAESQQIWVERGGFTSVNS
                     EVSLDTYPDEVARKQAQQLLEAPTFRFDLDDAIGGSVQQAIFNAIITYLQNPDDLDAL
                     LQNIEDARGG"
     gene            complement(2646..>2897)
                     /locus_tag="VNL92_07605"
     CDS             complement(2646..>2897)
                     /locus_tag="VNL92_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94614.1"
                     /translation="IDVIEPRAVVSLGSVALASLAAIEPHGLSLRQDVGRVVAWYGRL
                     LVPLYHPSPQTRAARSDAQQASDYRRLGLLLEPALAGTR"
BASE COUNT          550 a          907 c          924 g          516 t
ORIGIN      
        1 gacatcggca gggtcgcgga aggagctgat gaggagcgcg atggtcggga tgctccacag
       61 gacggccagc gcgatgacgg caaggtggag gggacttcgg aagctgaagc gacgcagcgc
      121 cttggcagca cgagctaccg cagatcccgt ccgttccgca ggtgtcaccg cgctcgtgtt
      181 cgccatcagg atgtctcctg ctggaactgg aagcggcgaa tgctgattaa catcagcggg
      241 accacgaaca gcatgagcac gatagcgagt gccgcgccca cgccgaagtc gcgaagcacg
      301 aacgcttcct tgaagaagag cgtcgcgacg acatcggtct gaaagcgccc gcccgtcatg
      361 acccagatga gatcgaagtt tttcagcgcc tggatgacga gggtcgtcgc gacgaccacg
      421 atggtgggcg aaaggatggg caaggtgatc cgggtgaaaa cttgccattc gttcgcgccg
      481 tccacccgcg ctgcctcaag cacctcggtc gagattcctt tgagtccggc cgagaggacc
      541 accatcgcga agcctgtcca catccatact cctaccgtga tgagcgcgaa cgtgttgatc
      601 tggagtggtc cggggagggt ttccggccca agggaggtga accacgcgta cggagcgttc
      661 gtgttctgca gccacgccgt tgggtcgccc ccaaggcccg acacgacggc attcaccgtg
      721 ccgatatcag ggtcgaactc gtacatgaac ttccagatga cggatgctgc tacaaaagag
      781 atcgccatcg ggatgaagat cgctgccttg gcaacgctct cgtagcgcac gcgacccgtc
      841 agctccgcga gcaccaggcc caggatgacg gtgaccgccg tgaagagcgc gagccagagc
      901 acggtgtgga ggagggctgc gtgcgtatcg gcgactatcg gattggggtt ctcaatgatg
      961 aagcgatagt tgtcgaggcc gacccatccc tccgaacggg cgtccagaaa gctccggcga
     1021 atcgtgtcga tcgtcggata gagcaggaac acagccagaa gaatgaaggc gggcgcgagc
     1081 catatccagg gcgcccacca ccgccgtccg atcatcggcc gcctcccgat gcagcccgcg
     1141 ccaagcccta gcctcctcga gcgtcctcga tgttctggag aagtgcgtcg aggtcgtctg
     1201 gattctggag gtaggtgatg atggcgttga agatggcctg ctgcacagag ccaccgatcg
     1261 cgtcgtccag gtcgaaccgg aaggtgggcg cttcgagcaa ctgctgggct tgcttccgcg
     1321 ccacctcgtc cgggtacgta tcgaggctga cctcgctgtt gacagaagtg aagccgccgc
     1381 gctctaccca gatctgctgc gattccgctc cagcgagcca gctgagaacc gagcacgtcg
     1441 caggatcgct attgaacgcg tacacgatgt tggctccgcc cgtaaccgct ccccccggga
     1501 acgggaagaa atcgtagtcc tcgccggcta ccgcgtccgg gaactgatcg gtgatgaagc
     1561 cggctgcgaa cccgccgaga tacaccatgg cggcctcggg aggatcgccg aacggcagca
     1621 gcgtggcatc ctggaagttc gtcgcgttga tgccctccgc gccgccctgg gcgacatatc
     1681 cctctgtcaa tgcgatctgg ccgaacttct cgaaagcatc ccgcacggcc gggtctgtga
     1741 aggggacgga cccgtcgacg acaccgtcgt acgtctgctc gcctgcctcg ttcaggagga
     1801 tctgctggat ccagtcgctg ccggggaagc cgctgcctgc gtccgcctcg ttgccgatcg
     1861 accagggcgg cacgccatgg tcgccggccg cgatttcgtc cgagagcgcg acgaggtcgt
     1921 cgaaggtgct gtcttcactg agcggctcgt acccgttctc ctcgaagaac tggggatcgt
     1981 accagatggt tcctttcgtg tcggccttca tgaagaagcc gtagagctcg ccatccacag
     2041 tgccgagctc gatgaaggat tgcggatagt tctgcatgat ctgctcctcg aggcccatgc
     2101 agctcgagag cgggaccagc tctccctccg cagcgaactg gcggaagagg cctatctccg
     2161 ccgggatcgc gatgtcgggc gggttgccgc cttcgacgcg cgtggtcaat agtgacgtga
     2221 tatcgcgcgt acccgtgaac tcgacactgc ctcccgtgtc gtcttgccag ggcgccacca
     2281 tcgcttcgaa cgacgcgagc tcttcgtcgc cccagatgcc gagtacgtcg acggattcac
     2341 cacctagatc ctcgcctgcg cccgctggag tcgtgccaga aggcgtggtg gtttcttctt
     2401 cgtcatcgtc atcgtcgtct ccacaagcta cggctagccc cgccgaaagc acagccacga
     2461 gaatgagaag catcagacgt gtcttgagca tgaatccccc ctgtttgacc accgtattga
     2521 tgctggtcat agcgttccca tccaccctac cacacgttta caaggctgcc gcggcgcgca
     2581 tcaaggccgt ttcgcaatgg cacgggggac cttcggcccg aggtcaggtg cgagcgcggt
     2641 ggcggtcatc tcgtcccggc gagcgcgggc tccaagagca ggccaaggcg acgataatcg
     2701 ctggcctgct gtgcgtcgct gcgcgcggcg cgagtttgcg gcgaggggtg gtacagcggg
     2761 acgaggaggc gcccgtacca ggctacgact cgacccacgt cttgtcgcag gctcaggccg
     2821 tgcggctcga ttgcagctag cgaagccagc gctacgctgc ccaggcttac gactgcccgc
     2881 ggctcgatga catcgat
//
LOCUS       DATMBB010000355         2972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_454152, whole genome shotgun sequence.
ACCESSION   DATMBB010000355 DATMBB010000000
VERSION     DATMBB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454152"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2564
                     /locus_tag="VNL92_07610"
     CDS             <1..2564
                     /locus_tag="VNL92_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HWO94615.1"
                     /translation="GDAAIVGQNVAFDLAFLARKGLVPPGPVFDTAELARVLLWDSRE
                     FSLRALAAYFGFAFPVRHRALPDAEMAMQVFLALRERALGLDNDVLVEVTRLASASPW
                     PAKELLAGLLAERTTALEGGPAQATNGRRLREAWPSLVPCEETRPVDEADVIATLARG
                     IDKAQAQGFERRPQQEQMASAVARALSEGSHLLVEAGTGTGKSLAYLVPAALFALRNQ
                     MRVVLSTNTIALQEQLVDKDIVLLRDMLDPSDRAALRVTQLKGRRNYLCLRRWAGMRH
                     GGPYTSEEAKLLARLAVWLPHTQTGDRAELNLTQAEEAVWSRVAAQEDCGPSCVAAAR
                     GGCYVQQARRRAEGAHLLIVNHALLLSDLATGGHLLPEYDHLVIDEAHHLEDEATSQF
                     GFEASHTQVATYLDKISAGRGGAAIGLAGRLRGIARAASGEPLGQGALAGIGSTIDAM
                     IEDVADARQQAAQFFAAIRSFVREHSEAPGDYETRLLVTSAVRRQPAWADVEIAWDAM
                     STALHGVIRAIEHASISLSDASALVDVDVTAVIDECTALIAEGTRLREGIASIVGRED
                     VDRIVWLTSSESGTATVQSAPLHTGDILRETLFEQRRSVILTGATLATSEGFHYVRER
                     LGLDDGRELLLGAPFDYRSSALVLVPSGIPEPGQPGYQRAVEQAIIALARASGGRALV
                     LLTSHAAVRATAAAIRPALEAEQILVLAHGIDGSPRQLIATLRENSRTVVLGTASFWE
                     GVDVVGDALSLLIIGKLPFAVPTDPVFAARSEQFDEPFSEYALPQAILKFRQGFGRLI
                     RRRTDRGVLVVLDRRVRSRQYGQAFLDALPDCIIEERRPGELAARVQDWLCPKGE"
     gene            2561..>2972
                     /locus_tag="VNL92_07615"
     CDS             2561..>2972
                     /locus_tag="VNL92_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWO94616.1"
                     /translation="MNVRRAEGEDRDVLQAWLADALSGASGSRTTAVSAAERLLSMIG
                     DRPQDLRIIEVDGRPEGLVKARDTRETCAFDAVAVRPSARGGGIAAKAILEIEDSSRA
                     SRFVGALAPADGRALYFWLRLGYRPVRRPDGRVEM"
BASE COUNT          464 a          969 c         1041 g          498 t
ORIGIN      
        1 tcggtgatgc cgcgatcgtc ggtcagaatg tcgccttcga cctcgccttt ctggctcgca
       61 agggcctcgt gccgcccggt ccggtcttcg atacagcgga gctcgcgcga gtattgctct
      121 gggactcgcg agaattcagc ctgcgggcat tggctgccta ctttggcttt gcgttccccg
      181 tccggcacag agcactgcct gatgccgaga tggcgatgca ggtcttcctg gcgctgcgag
      241 aacgcgctct cgggctcgac aacgacgttc tggtcgaggt gacgcgtctc gcttcggctt
      301 cgccgtggcc cgcgaaggag ctcctcgccg ggctgctcgc cgagcgcaca acggctctcg
      361 aagggggtcc tgcacaggcg acgaatgggc gccgccttcg ggaggcatgg ccatcgctcg
      421 tgccgtgcga ggagacccgc cctgtcgacg aagcggacgt gatcgccacc ctcgcccgcg
      481 gcatcgacaa ggcgcaggct cagggcttcg agcgacgtcc ccagcaagag cagatggcat
      541 cggccgtagc tcgcgccctc agcgaagggt cgcatctgct cgtggaagcg ggcacgggca
      601 ccggcaaatc gctggcctac ctcgtgccgg cggcgctgtt cgcgctgcgc aaccagatgc
      661 gcgtggtcct ttccacgaac accatcgcct tgcaggaaca gctcgtcgac aaggacatcg
      721 tactgttgcg agacatgctc gacccgagcg atcgtgctgc gcttcgcgtc acccagctga
      781 agggtcgacg caactacctg tgcctccgcc gctgggcagg gatgcggcac ggaggcccgt
      841 atacgagcga agaggcgaag ttgctcgccc ggctcgccgt atggctgccg cacacgcaga
      901 cgggcgacag ggcggagctg aacctcacgc aggccgaaga ggccgtgtgg tcgcgcgtgg
      961 ccgcccagga ggattgcggg cctagctgcg tcgccgccgc gcgtggtggc tgctatgtgc
     1021 agcaggcgcg gcgccgggca gaaggtgcac acctcctcat cgtcaaccat gcgctgctgt
     1081 tatcggacct cgcgaccgga ggccacctgc tgccggagta cgaccatctc gtcatcgatg
     1141 aggcgcatca cctggaggac gaagcgacga gccagttcgg cttcgaagcg tcgcacaccc
     1201 aggtggccac ctatctggac aagatctcgg cggggcgggg cggcgccgcg atcggtcttg
     1261 cggggcgtct gcgcggtatt gcgcgcgccg cgagcggcga gcccctcggc caaggcgcgc
     1321 tggccggcat cggttcgacg atcgatgcca tgatcgagga cgtggctgac gcccggcagc
     1381 aggcagcgca gttcttcgct gcgattcgaa gcttcgtccg cgagcactca gaagcgccgg
     1441 gcgattacga gacgaggctc ctcgtcacga gcgccgtacg ccggcagccg gcgtgggccg
     1501 acgtcgagat cgcgtgggat gcgatgtcca cagcgctcca cggcgtcatc agggcgatag
     1561 agcatgcgtc gatctcgctc agcgatgcct ccgcgctcgt ggatgtcgat gttacggccg
     1621 tgatcgatga atgcacggcg ctgatcgccg agggcacccg gcttcgcgag ggcatcgcgt
     1681 ccatcgtagg gcgggaggat gtagacagga tcgtgtggct cacctcctcc gaatcgggga
     1741 cggcgaccgt gcaatcggcc cctctgcata cgggcgacat cctgcgcgaa acgctcttcg
     1801 agcagcgccg cagcgtcatt ctcacgggcg caacgctggc tacgtccgag gggttccatt
     1861 acgttcggga acgactgggg ctcgacgatg gacgcgagct cctgctcggc gctccgttcg
     1921 actaccgatc ctcggcgctg gtgctcgtcc cttcaggaat cccggagccg ggacagcccg
     1981 gctaccagcg tgctgtggag caggccatca tcgcgcttgc gcgggcctcc ggagggcgag
     2041 ccttggtact gctcacgtcg catgcggcgg tccgcgcgac ggcggcagcc atccggccgg
     2101 ccttggaggc ggagcagatc cttgtgctgg cgcacggcat cgacggctcg ccgcggcagc
     2161 tcatcgcgac gctgcgcgaa aacagtcgca cagtcgtctt gggcacggcg agcttttggg
     2221 agggcgtcga cgtcgtgggc gacgcgcttt cgctcctgat catcggcaag ctgcccttcg
     2281 ccgtgcccac cgaccccgtg tttgcggccc gatcggagca gttcgacgag ccgttttccg
     2341 aatacgcgct gccgcaggcc atccttaagt tccgccaggg gttcggcagg ctgatccgcc
     2401 ggcggacgga ccgaggcgtg ctcgtggtcc tcgatcgccg cgtgcgcagc cggcagtacg
     2461 gacaggcctt tttggacgcg ctccccgact gcatcatcga ggagcggcgg ccgggcgagc
     2521 tggcggcccg ggtgcaagac tggctgtgtc cgaagggcga atgaacgtcc gccgcgccga
     2581 aggcgaggac cgcgacgtgc tacaggcctg gctcgcagac gcgttgtcgg gcgcaagcgg
     2641 atcccgaacc acagcggtct cggcagcgga gcgcctcctt tcgatgattg gcgatcgtcc
     2701 gcaggacttg cgcatcattg aagtggatgg aaggccggag ggcttggtca aggcacgtga
     2761 cacccgcgaa acgtgcgcgt tcgacgcggt cgccgttcgt ccgtccgccc gagggggcgg
     2821 gatagccgcg aaggcgatcc tcgaaataga ggacagttca cgtgcgagtc gcttcgtcgg
     2881 agcgttggcc cctgcggacg ggcgggcgct ctacttctgg cttcggctag gctatcggcc
     2941 ggtgcgacga ccggacggac gtgtcgagat gg
//
LOCUS       DATMBB010000356         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_218972, whole genome shotgun sequence.
ACCESSION   DATMBB010000356 DATMBB010000000
VERSION     DATMBB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218972"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VNL92_07620"
     CDS             <1..1185
                     /locus_tag="VNL92_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HWO94617.1"
                     /translation="VIRDGPTIVARLDVGTLAEAPVYVREPRPSEEMLRHRRMDLAAL
                     PDTRDPSADLLRLLASPNVASRRLVYRQYDHQVGTNTVVLPGEGDAAVLRIRGTARAI
                     AVATDGNGRIAALDPFAGGAAAVAEACRNVVCAGGEPVALTDCLNFGNPERPEVYYQL
                     SRAIDGMAEASRALGAPVVSGNVSLYNESGGTAVHPTPVVGALGLLEDVSHATRKAFA
                     ESGCIVYLLGTPLRGDPSLLAASEYLNVAGHELGGQPAIDLDLEARLQRCTLAAIRSG
                     LVRAAHDCSEGGVAVALAEACIAGGLGLDASSVVEDDRIDAFWFGEGQSRIVMAIESG
                     DEVRLSALAMEHDVPLSRLGVVGGDRLRLGSRIHLSLATARAAYERGLEQALATVEAG
                     VA"
     gene            1349..1945
                     /gene="rplI"
                     /locus_tag="VNL92_07625"
     CDS             1349..1945
                     /gene="rplI"
                     /locus_tag="VNL92_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWO94618.1"
                     /translation="MKVVFLEHVEGTAYPGDVKDVADGFARNYLLPRGFAVPATKSAL
                     QRAEALQKVEERRQAKLDDEAQALRERLEGHTLRFEERVGEQGRLFGSVTVSHIAEKV
                     SQLLGEEFDRRKVLLADPLRQLGRHEVRLRLSRNVEHVIPVDVVPLGETEPPVPQEAP
                     SMASGAAAPVMEREAESAAEVAVEEVDAVEEATEEAEG"
     gene            1984..>2359
                     /locus_tag="VNL92_07630"
     CDS             1984..>2359
                     /locus_tag="VNL92_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HWO94619.1"
                     /translation="MVIEKLPPHDTEAEEAVLASLLVDPEAVARVAGTLRPNDFFTQR
                     NGWVYQACLALWERNEAVNELTVAHELARAGQLEEAGGLTYLSRIIAELPTPVGVEHY
                     ARIVERDALYRRLIGISGQIAQM"
BASE COUNT          377 a          756 c          837 g          389 t
ORIGIN      
        1 gtgattcgtg atggcccgac gatcgtggct cgactcgacg tgggcacgct ggcggaggcg
       61 ccggtctacg tgcgagaacc tcgcccctcg gaggagatgt tgcgccaccg gcgcatggac
      121 ctcgccgcac tccccgatac gcgcgatccg agcgccgact tgctacgcct tctcgcctca
      181 ccgaacgtcg ccagccggcg gctcgtctac cgccagtacg accaccaagt cgggacgaac
      241 acggtcgtgc tcccgggcga gggcgacgcg gccgtgctgc gaattcgcgg cacggcccgg
      301 gcgatcgcgg tggccacgga tgggaatggc cgcattgctg cgctcgatcc gttcgctggg
      361 ggggcggcag cggtcgccga ggcgtgccgc aacgttgttt gcgccggagg cgagcccgtc
      421 gccctcaccg attgcctgaa cttcggcaac ccggagcgtc ccgaggtgta ctaccagctc
      481 tcgcgcgcaa tcgatggcat ggccgaggcc tcgcgagcgc tgggagcgcc ggtcgtctcc
      541 ggcaacgtca gcctctacaa cgagtcgggc ggaacggccg tccaccctac gccggtggtg
      601 ggtgcgctgg gtcttctcga agatgtgtcg cacgccacac ggaaggcgtt cgctgaatcg
      661 gggtgcatcg tctacctgct ggggacgccg ctccgtggcg atcccagcct gcttgccgcg
      721 tcggagtatc tgaacgtcgc cggtcatgag ctcggcggcc agccggccat cgatctggac
      781 ctcgaggcgc ggcttcaacg ctgcacccta gcggccatcc gctcgggctt ggtccgcgcc
      841 gctcacgatt gcagcgaagg cggggtcgcc gtagcgctcg ccgaggcgtg catcgcggga
      901 ggcctgggtc tggacgcatc ctcggtcgtc gaagatgatc gtatcgacgc cttctggttc
      961 ggcgaggggc agtctcgcat cgtgatggcc attgagtccg gcgacgaggt tcgcctgagc
     1021 gccctcgcga tggagcatga tgtgccgctg tcccgcctcg gtgtcgtcgg cggcgatagg
     1081 ctccgcctcg ggtcgcgcat ccacctgtcc ctcgcgacgg ctcgcgctgc ttacgagcgc
     1141 ggcctcgagc aggcattggc gacggtcgaa gcgggcgtcg cctgatcgag gctcttggga
     1201 gcctgtcggc tgcctccttg acgtaagaac gaatgtgcgt ctaagctcag gtaacggcgc
     1261 cggcaggaaa aacgctaggt cgccataaga agctatgcat caacgcgccg gcgtcggata
     1321 atccgcgaac ggcaacgagg agtggttcat gaaggtcgtg tttctggaac acgtggaggg
     1381 caccgcgtac ccgggcgatg tcaaggacgt cgccgatggc tttgctcgaa actatctcct
     1441 accgcgcgga tttgccgtcc cggcaacgaa gagcgcgctg cagcgggccg aggcgctgca
     1501 gaaggtggag gaacgccgcc aggccaagct cgatgatgag gcgcaggcac ttcgggagcg
     1561 gttggagggc cacaccctcc gcttcgagga gcgtgtcggc gagcagggca ggctgttcgg
     1621 ctcggttacc gtgtcgcaca tcgcggagaa ggtgagccag ctgctcggcg aggagttcga
     1681 ccggcggaag gtgctccttg ccgacccgct acgtcagctt ggccggcacg aggtgcgatt
     1741 gcgcctcagc cgcaacgtcg agcacgtcat tccggtcgat gtcgttccgc tgggcgagac
     1801 ggagcctccg gtcccgcaag aggcgcccag catggcgagt ggcgcggccg cgccggtgat
     1861 ggagcgcgag gccgagagcg ctgcggaggt tgcggtggag gaagtggacg cggtggagga
     1921 agcaacggaa gaagccgagg gctaatcggc gggtcccccc gcggaaggac cggtgggtag
     1981 cgcatggtca tcgaaaagct gcctcctcat gacacggaag cggaggaagc tgtcctcgcc
     2041 tctctgctgg tcgacccgga ggccgtggcc cgtgtcgccg gaaccctccg ccccaacgac
     2101 ttcttcactc agcggaatgg ctgggtctac caagcctgtc ttgccctttg ggagcgcaac
     2161 gaggcggtca acgaactgac cgtagcccac gagctggcgc gcgcgggcca gctcgaggaa
     2221 gccggcggcc tcacgtacct gtcacgcatc atcgcggagc tgccgacgcc cgtcggcgtc
     2281 gagcactacg cgcggattgt cgaacgcgat gcgttgtacc ggcggctcat cggtatctct
     2341 ggccagatcg cgcagatgg
//
LOCUS       DATMBB010000357         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_57603, whole genome shotgun sequence.
ACCESSION   DATMBB010000357 DATMBB010000000
VERSION     DATMBB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57603"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1686
                     /locus_tag="VNL92_07635"
     CDS             16..1686
                     /locus_tag="VNL92_07635"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWO94620.1"
                     /translation="MTTLEKTGTKTDSGARPDFDAVVVGAGFSGLYMLRRLRDVLGLS
                     ARVYEAGGDVGGTWYWNRYPGARCDSESIYYCFSDNVSEELLQEWTWSERFAAQPEIL
                     RYLQFVADRLDLRRDIQFNTRITAAHWDEAANLWRIHADDGTVVTATYFISALGCLSQ
                     KNLPDFKGLDSFQGDWYHTGAWPHEGVDFTGKRVAVIGTGATAVQVVPEVAKQASHLY
                     VFQRTPNYDIPGENRPLDEAYMQSVKASYREIWRKARDAGNGFPYQVEDRSALSVTPE
                     ERQAIFEAAWKQGGFYVGFGTFNDFLLSKEANDTIAEFIRSKIRETVHDPEVADLLSP
                     KDYPFLTKRPPLEHGYYEAFNRDNVTLVDIRRSSIEEITPRGVRAGGKEYEVDSIIFA
                     TGFDAMTGALLAIDIRGTGGQTLKEHWADGPLTYLGLATNGFPNMFTITGPQSPSVLT
                     NMPVAIEQHVEWISDCIAYMREHGLSRIEAQREAEDGWVDHANQIANMTLLLQTDSWW
                     VGANIPGKKRIAYPYVGGLGNYRKVCDDVAANGYKGFELSAEQVTSKA"
     gene            1784..2143
                     /locus_tag="VNL92_07640"
     CDS             1784..2143
                     /locus_tag="VNL92_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94621.1"
                     /translation="MIPVYIEIGTKRVFADAISWPGWCRSARTEGEALATLLAYGKRY
                     AKATASSDVRFVPPASVDEFEVPIVPLWLRPIRPVAKPSTCFVHAHTARSRPPARVGR
                     GPSGVRDSTSAMRRGTH"
     gene            complement(2244..2912)
                     /locus_tag="VNL92_07645"
     CDS             complement(2244..2912)
                     /locus_tag="VNL92_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWO94622.1"
                     /translation="MTAGLRFRDRTDGGRRLAEALTQYREERPLVVGVLRGGAVIAAE
                     VAGQLGAEMDVIGVRKIAGPAMRELALGAVAADGTRLLNDDIIAEMGIAPAYVEGEAE
                     LGQAAAHRQEQYLRGEHHMARVKDRTVIVVDDGAATGATMRTALRSLAKKGPAKLVAA
                     IPVASGEALQAIVGEGAKVVCLAQPAALGSVGEYYEQFEPVTDSEAAEILRREYRRRE
                     AGRS"
     gene            3184..3612
                     /locus_tag="VNL92_07650"
     CDS             3184..3612
                     /locus_tag="VNL92_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO94623.1"
                     /translation="MYERIVVPLDGSAEAEAALPVAEALARRPGAHVQLVHIVDPRVI
                     AGLNLADTAALRTEQVCQADDYLSRIGAQLRTKGVAADWDVRLGDPPRALLRAIRDVE
                     ADLVIVVRRGAGRSDEAGSVAMKLLRQAACPAIMVPAPQT"
     gene            3620..>4082
                     /locus_tag="VNL92_07655"
     CDS             3620..>4082
                     /locus_tag="VNL92_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94624.1"
                     /translation="MSRESLRRWRGQAWSAALILSGASIALGILAARYDRFPLDSWLT
                     NRVGRIGAWFEQPAEAACALATPVAIFAGVAGAAFAVARRHGELAALFGSLLIVRPLL
                     SLARLPIERPRVSDPQDLLSFPSGHVMTAALPLGFWLVFGGALVSARTATLI"
BASE COUNT          690 a         1376 c         1298 g          718 t
ORIGIN      
        1 cagggggaga tacccatgac tacattagag aaaactggta ccaaaaccga ttccggtgca
       61 cgcccggact tcgatgccgt cgtcgtgggt gcaggattct ccgggctcta catgctgcgc
      121 cgcttgcgcg atgtcctcgg cctctcggcg cgggtgtatg aggccggcgg cgacgtggga
      181 ggtacatggt actggaaccg atatcccggc gcccgctgcg atagcgaaag catctactac
      241 tgcttctccg acaacgtttc ggaggaattg ctccaggagt ggacgtggtc ggagcgcttc
      301 gccgctcagc ccgaaatcct gcgttacctc cagttcgtcg ccgaccggct cgatctacgg
      361 cgcgatatcc agttcaacac gcgcatcacg gccgctcact gggatgaagc tgcgaacctg
      421 tggcgaatcc atgcggacga tggcacggta gtgacggcga cctacttcat ctccgccctc
      481 ggttgccttt cgcagaagaa cctgcccgac ttcaagggcc tcgactcgtt ccagggcgat
      541 tggtatcaca ccggagcctg gccgcacgag ggtgtcgact tcacgggcaa gcgagtcgcc
      601 gtcattggca ccggcgccac ggcggtccag gtcgtgccgg aggtcgcgaa gcaggcgtcg
      661 cacctctatg tgttccagcg cacaccgaac tacgacatcc ccggggagaa ccggcccctc
      721 gacgaagcgt acatgcagtc ggtcaaggcc agctaccgcg agatctggcg gaaggcccgt
      781 gatgccggca acggcttccc ctaccaagtc gaggataggt ctgccttgtc ggtgacgccc
      841 gaggagcggc aggcgatctt cgaggcggcc tggaagcagg gcggcttcta tgtcgggttc
      901 ggcacgttta acgacttcct cctgagcaag gaggcgaatg acaccatcgc cgagttcatc
      961 cgctcgaaga tccgcgagac cgttcacgat cccgaagtgg ccgacctgct ctcgccaaag
     1021 gactacccgt tcctcacgaa gcgccctccg ctcgagcacg gctactacga ggcgttcaac
     1081 cgcgacaacg tcacgcttgt cgacatccgc cggtcgtcca tcgaggagat tactccgcgc
     1141 ggagtgcgtg caggcggcaa ggaatacgag gtcgatagca tcatcttcgc gaccggattc
     1201 gacgcgatga ccggagccct gctcgcaatc gacatacggg gcacaggcgg ccagacgctc
     1261 aaggagcatt gggccgatgg gccgttgacg tacctgggct tggcgacgaa cggcttccca
     1321 aacatgttca ccattaccgg tccgcagagc ccttccgtgc tcaccaacat gccggtggcc
     1381 atcgagcagc acgtcgagtg gatctccgac tgcatcgcgt acatgcgaga gcacggactg
     1441 tcgcgcattg aggcgcagcg cgaggccgag gacggctggg tggaccacgc gaaccagatc
     1501 gcgaacatga ccctgctgct gcaaacggac tcctggtggg tgggcgccaa catcccaggg
     1561 aagaagcgta tcgcgtaccc atacgtcggc ggcctgggga actatcgtaa ggtctgcgac
     1621 gacgtcgccg cgaacgggta caaggggttc gagttgagtg cggagcaggt gacgagcaag
     1681 gcgtaacgtc cgcctacgtc gccgcgtctg cgcggcgcag tctgactttc cgggaagcgg
     1741 ccgcgaaatg cggccgcttc tgcgcaacat acggaggcgt tccgtgattc ccgtgtacat
     1801 cgagatcgga acgaagcgcg tgttcgccga cgccatctcg tggccgggat ggtgccgcag
     1861 cgcgcgaacc gaaggcgagg ccctcgcgac gctgcttgcc tacggcaaac gttacgcgaa
     1921 ggccaccgcg tcctccgacg tgcgattcgt ccctccagcg agtgtcgacg agttcgaagt
     1981 gccgatcgtt cccctttggc tgcggccaat acgacccgta gcgaagccat ccacctgctt
     2041 cgttcacgcg cacacggcga ggagccggcc accggcccgc gtcggacgcg gcccttctgg
     2101 agtccgcgat tctacatccg ccatgcggcg tggcacgcac tagaccatac ctgggaaatc
     2161 gaggaccggt cgacatgaca cggcacccca gtttcgtgcc gtcgtgcacg acggatctgc
     2221 cgtaagggcc tggctttgcc ggtctaggat ctcccggcct cgcgcctacg gtattcacgc
     2281 cgcagaatct cggcagcttc gctgtcggtc actggctcga actgctcgta gtactcgccc
     2341 accgacccga gggccgccgg ctgggcgagg caaaccacct tggccccttc gccaactatc
     2401 gcctgcaggg cctccccgga ggcgacggga atggccgcga cgagcttcgc cggccccttc
     2461 ttcgcgagag atcggagcgc cgttcgcatc gtcgcgccgg tggccgcgcc gtcgtcgacg
     2521 acgatgactg ttctgtcctt gacccgggcc atgtggtgct caccgcgaag gtactgctcc
     2581 tgacgatgcg ccgccgcctg gcccaactcc gcctctccct cgacgtatgc tggtgcgatg
     2641 cccatctcgg cgatgatgtc gtcgttcaga aggcgcgtgc catcagccgc gacggccccc
     2701 aaagcgagct cgcgcatggc agggcccgct atcttccgga cgccgatgac gtccatctct
     2761 gcgcccagct ggcccgccac ttccgcggca atcactgccc cacctcggag cactccaacc
     2821 accaacgggc gctcttcccg atactgcgtc agcgcctccg cgagacgacg ccctccatca
     2881 gtccgatcgc gaaaacgcag gcctgctgtc atggtcttgc ctccactcca agatgaaagt
     2941 cttcgcgcgc cgatcctgat tcattatccg gtgagcggcc gtgtgctgta gtgccatgtg
     3001 gccctaggcc cgctgccgga tgactccgca gcttcggccg cggtcgtgac actcggcccc
     3061 cacgaaagct ccgttcggcg ctcccgtccc gtcacggatg acggcactat ctgctcgata
     3121 ggcgctgcgc gaccgagcgc gtgcgaccaa ctgcccagaa tcgcggctcc gggaggagga
     3181 tccatgtacg aacggatcgt agtcccactc gatggatcgg cggaggcgga agcggcgctc
     3241 cctgtcgccg aggcgctcgc acgccgcccc ggcgctcacg tccaattggt ccacatcgtc
     3301 gacccgcggg taatcgctgg actcaacttg gcggacacgg cggcactccg caccgagcaa
     3361 gtgtgtcagg ccgacgacta cctgagccgt atcggggcgc aattgcgtac gaagggtgtc
     3421 gcggccgact gggacgtgcg gctgggcgac ccgccacggg ccttgcttcg agccatacga
     3481 gacgtcgaag ccgacctcgt catcgtcgtt cgtcgcggtg ccggccggag tgacgaggcc
     3541 ggcagtgtgg cgatgaagct gcttcgccag gcagcgtgcc cggcgatcat ggtgccggcg
     3601 cctcagacgt gactggccgg tgagccgcga gtccctgcgg cgctggcgcg gccaggcgtg
     3661 gtccgcggct ctgatactga gcggcgcgtc gatcgcgctc ggcatcctcg ccgcgcgcta
     3721 cgaccggttt ccgttggata gctggctgac caatcgcgtc gggcgcatcg gcgcctggtt
     3781 cgaacagccg gctgaagcgg cctgcgcgct agcgacgcct gtcgccatct tcgccggcgt
     3841 agccggtgct gcattcgcag tcgcgcgccg ccacggggag cttgcggccc tcttcgggtc
     3901 actgctgatc gtgaggcctc tgctgtcgct ggctcgcctg ccgattgaac gtcctcgcgt
     3961 ctcggatccg caggacctct tgtcgttccc gagcggccac gtgatgacgg cggcgctgcc
     4021 tctgggattc tggctggtgt tcggcggcgc gcttgtctcc gcgagaacgg cgaccctcat
     4081 tc
//
LOCUS       DATMBB010000358         2055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_296905, whole genome shotgun sequence.
ACCESSION   DATMBB010000358 DATMBB010000000
VERSION     DATMBB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296905"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1318)
                     /gene="mazG"
                     /locus_tag="VNL92_07660"
     CDS             complement(236..1318)
                     /gene="mazG"
                     /locus_tag="VNL92_07660"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HWO94625.1"
                     /translation="MELTAFGSACRAFGIGSLGAGLHLAFGSAPSFDPAVASLLLDVR
                     RDDMAQLCRSLRQRYPATHSVRILDEDGVREVDLAHLETAMDGEQASVLVPPLAAEDD
                     VRDFRGLVAIIRRLRDPEPGCPWDRQQTHASLKRYLLEEAYETLDALDRDDAAALREE
                     LGDLLLQIVLHARIAEQSDEFETADVVEAISAKLIRRHPHVFGDVTAHTARDVEQRWD
                     ELKRAESADGSSTSALGGVPRAMPALAYSQAILGRAERAGFAWPGTDDVLAKVAEEAR
                     ELAGARDVDERREEFGDLLFALVSLARRLDVDAEESLRLAATKFRSRFEALESMAREE
                     RVSLASLDPKPLLALWERAKEQAAEA"
     gene            1422..>2055
                     /locus_tag="VNL92_07665"
     CDS             1422..>2055
                     /locus_tag="VNL92_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HWO94626.1"
                     /translation="MAIAHRAASALFVVALPILLITTNVRFAGTETRLWEWGFDRYDA
                     EGTTGIDRPELDRAADQIAAYFTNDEPYLRIKVEDDGRLQPLFNQRETAHMADVKDLF
                     GLTFAVQAVALAYVLSYVVGVFVWEQASPLGVLARQVLLSLAVLAGAVIVIGGIALVG
                     FDAAWEQFHIIGFDNDLWQLDPDTDHLIQMFPEDFFFDMTLLVGGLTLLEA"
BASE COUNT          341 a          659 c          695 g          360 t
ORIGIN      
        1 tccatcaggg cacggaactc agcgatttcc tccgctgtcg gccgaaaccg gcggtagtcg
       61 cgttcgtaca ggccgcggta ccgttctcct atctggctca cgtactcgcg gtagctcatc
      121 ccctgcgcga agcgctccgg agtgacgaca gagttcgttg cgaccatgaa tccacctcct
      181 ggtgcttgaa agtgtaacgt gcccgtcgac gccgtcacaa gaccgatggg cgacctcatg
      241 cttcagccgc ttgctccttg gctcgctccc ataacgctag gagcggctta gggtcgagcg
      301 acgcgagcga gacgcgctcc tcccgagcca tcgactcgag cgcctcgaac cgggagcgga
      361 acttcgtggc ggcgagtcgt agcgattctt ccgcatccac atcgagccgc ctggcgagcg
      421 aaacgagcgc gaacagcagg tcgccgaact cctcgcggcg ctcgtcgacg tcccgggctc
      481 cggcgagctc gcgggcctcc tcggccacct tggccagcac gtcatccgtt cccggccatg
      541 cgaagccggc ccgttcggcg cgaccgagga tggcttggct gtaggcgagc gccggcatcg
      601 cgcgcggaac gccgccgagc gcagaggtcg aagacccgtc ggcggactcg gctcgcttga
      661 gctcgtccca ccgttgctcg acatcccgtg ccgtgtgcgc cgtgacgtcg ccaaagacgt
      721 ggggatggcg acgaatcagc ttcgccgata tagcctccac gacgtcggca gtctcgaact
      781 cgtccgattg ctccgcgatc cgggcgtgca ggacgatctg caggagcagg tcgcccagtt
      841 cctcccgaag cgccgccgca tcgtcgcggt cgagcgcgtc gagcgtctcg taggcctcct
      901 cgaggaggta acgcttcagc gaggcgtgcg tctgctgacg atcccagggg caaccgggct
      961 cgggatcgcg cagacggcgg ataatggcga cgagcccgcg aaagtcgcgc acgtcgtcct
     1021 cggcggccag cggaggaacg agaacgctcg cttgctcgcc gtccatggcg gtctcgagat
     1081 gggcgaggtc gacctcccga acgccgtcct cgtcaaggat gcgaaccgaa tgcgtggccg
     1141 gatagcgctg tcgcaaggag cggcatagct gcgccatgtc gtcgcggcga acgtcgagga
     1201 ggagcgaagc gaccgcgggg tcgaaggacg gggcggatcc gaaggcgagg tgcaatcccg
     1261 cgccgagcga gccgatgccg aaggcacggc acgccgatcc aaaggcggtg agttccatga
     1321 gagcctctcg ccgagtatac gtgggccttc gtcgcgttga caatgaatct gagcggctcg
     1381 taggctgagg cttagacttg gcggtggttt ggggaggcag ggtggcaatc gctcacaggg
     1441 cggcgagcgc gctcttcgtg gttgcgctgc ccatcttgct catcacgacg aacgtgcggt
     1501 tcgctggtac cgagacgcgc ctctgggagt ggggcttcga tcgctacgac gcggagggga
     1561 cgacgggaat cgatcgtccg gagctcgatc gcgccgccga ccagatcgct gcctacttca
     1621 ccaacgacga gccgtacctc cgcatcaagg tggaggacga tgggaggctg cagccgctct
     1681 tcaatcagcg cgagacggcg cacatggccg acgtgaagga cttgtttggt ctcaccttcg
     1741 ccgtacaggc ggtggccctc gcgtacgttc tgagctacgt cgtaggcgtg ttcgtctggg
     1801 agcaggcgag tccgctaggc gtgttggcgc ggcaggttct cctgtcgctc gccgtgctcg
     1861 ccggcgcggt catcgtgatc ggtggcatcg cgctcgtcgg gttcgacgcg gcgtgggagc
     1921 aattccacat catcgggttc gataatgacc tgtggcaact cgaccccgat acggaccacc
     1981 tgatccagat gttcccggag gacttcttct tcgatatgac gctgctcgtc ggtggcctca
     2041 ccctgctcga ggcgg
//
LOCUS       DATMBB010000359         2820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_573685, whole genome shotgun sequence.
ACCESSION   DATMBB010000359 DATMBB010000000
VERSION     DATMBB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573685"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /gene="priA"
                     /locus_tag="VNL92_07670"
     CDS             complement(<1..2131)
                     /gene="priA"
                     /locus_tag="VNL92_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWO94627.1"
                     /translation="MSHLAAGTRFAEVAVNAHFPHRDTYTYAVPEGLDVRAGHAVYVP
                     FGRRRLQGVVLDLGDAPRFDPERIVPIEGLIEPEPLLSPEQVALVRWLVEEYLAPPFP
                     CARMFLPPGFEERPLTFYAALPLEMRRGGISERHQAVWNYLKENGPIEAAAAKAALRE
                     ATPAVLDSLVRKGLAIRTFGLAPARGKVRSVPHVRLIADRARVDAWLAHPRGEKPSRK
                     AALLELLAEQGGIDAAAARSSFGEALLRELAERHIVSIDAAARVTLSVTAGQAFDLAQ
                     DLRLTAGQKAQAVVLRLLLDEGPVLPRAVVRARTGINMPALRELETEGLIVLEDVPVE
                     RDPLAGRTFESRPAPTLTRGQADALRPIIAALERREPKRFLLHGVTGSGKTEVYLAAL
                     ERALAVGRRGIVLVPEIALTPQMIRRFAERFPGRFTVLHSGLSPGQRFDQWWRVARGE
                     VDIVIGARQALFAPIRDLGIIILDEEHEWTYKQHDAVPRYHARDAALQLGNLTGAVVL
                     LGSATPDVVSYHRAKEGAYRLLTLPERIAAHEHERDGGTSGSRALPDVEVVDMREELR
                     AGNREAFSRALHTAIEETLARGEQIILFLNRRGSSSFLLCRDCGEAPQCSSCSMALSH
                     HESPPRLVCHQCGRSRSVPERCPKCGGVRLRGVGIGTQRLEELVSLTFPRARILRWDR
                     DVTRVQGAHERILGQFLERQADILIGTQ"
     gene            complement(2181..2540)
                     /gene="rplS"
                     /locus_tag="VNL92_07675"
     CDS             complement(2181..2540)
                     /gene="rplS"
                     /locus_tag="VNL92_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWO94628.1"
                     /translation="MPDLTATTSLIDYRPNANVPEFGPGDTVRVHVKVVEGDRERVQA
                     FEGVVIRMRRAGASSNFTVRRVASHSVGVERTFLTHSPRIDKVEVLRRGKVRRKALYY
                     LRGRTGKKARIKEMGRT"
     gene            complement(2593..>2820)
                     /locus_tag="VNL92_07680"
     CDS             complement(2593..>2820)
                     /locus_tag="VNL92_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWO94629.1"
                     /translation="LLEYPQYTRPPEFRGMRVPDILLSGHHAEVAKWRRWQRILRTAQ
                     RRPDLLAQARLSEEERRQAEEALSEPASDKS"
BASE COUNT          458 a          947 c          969 g          446 t
ORIGIN      
        1 tctgcgtacc gatgaggatg tccgcttgcc gctcgaggaa ctgccccaaa atgcgctcgt
       61 gtgcgccctg cacgcgggtc acgtcccggt cccagcggag gatgcgcgcc cgcggaaacg
      121 tcaggctcac cagctcctcg aggcgctgcg ttccgatgcc gacgccgcga aggcgcactc
      181 ctccgcactt cgggcaacgc tccgggacag agcggctcct tccgcactgg tgacagacca
      241 atcgcggcgg gctctcgtgg tgcgatagtg ccatgctgca ggacgagcac tgcggcgcct
      301 cgccgcaatc gcggcagagc aggaacgacg agctgcctcg ccggttcagg aagagaatga
      361 tctgctcgcc cctcgccagc gtctcctcga tagccgtgtg gagggcgcgg ctgaaggcct
      421 cgcgattgcc ggcgcgcagc tcctccctca tatcgaccac ctcgacatcg ggcagcgcgc
      481 gggagccgct cgtgcctccg tcgcgttcgt gctcgtgcgc cgcaatccgt tctggcaggg
      541 tgagcaagcg ataggcgccc tccttggctc ggtgatagct cacgacgtcg ggagtcgcgc
      601 taccaaggag taccaccgcg ccggtcaggt tgccgagctg cagggcggcg tcgcgggcgt
      661 ggtaacgggg aacggcatcg tgctgcttat acgtccactc gtgctcctcg tccaggatga
      721 tgatgccgag gtctcggatg ggggcgaaca aggcctgccg agcccctatg acgatgtcca
      781 cctcgccccg ggcgacacgc caccactggt cgaatcgctg gccgggagac agccccgaat
      841 ggaggactgt gaacctccct gggaagcgct cggcaaagcg ccggatcatc tgcggggtga
      901 gggcgatctc cggtacgagc acgatgcctc tgcgtccgac agccagggcg cgctcgagcg
      961 cagccagata gacctccgtc ttgccgctgc cggtgacgcc gtgaaggagg aaccgcttcg
     1021 gctcgcgtcg ttccaaggca gcgatgatgg ggcgaagggc gtcggcctgc ccgcgggtca
     1081 gcgtcggcgc cgggcgcgac tcgaacgttc tgccggcgag cggatcgcgc tcgaccggca
     1141 cgtcttcgag gacgatgaga ccctccgtct cgagttcgcg aagcgcgggc atgttgatgc
     1201 cggttcgcgc ccgcacgacg gcgcgcggta gcacggggcc ttcgtccaac agcagccgca
     1261 ggacaacggc ctgagccttc tgacccgccg tcagccgaag gtcctgcgcc aggtcgaacg
     1321 cctgtcccgc cgtcacgctg agtgtcacac gagccgctgc gtcgatggac acgatatgac
     1381 gctcggcgag ctcccggagg agcgcctcgc cgaacgagga gcgggccgcg gcagcgtcga
     1441 tgccgccttg ctcagcgagg agctcgagca gggcagcctt acgcgacggc ttctcgcccc
     1501 ggggatgcgc gagccacgcg tcgacccgcg ccctgtcggc gatcaggcgg acgtgcggaa
     1561 cggagcgaac cttaccccgg gccggcgcca ggccgaaggt ccgaatcgca agtcccttgc
     1621 gcacgaggct atccagcacg gcgggcgtcg cctcacgcag ggcggccttc gccgcggcgg
     1681 cctcgatggg cccgttttcc ttcaggtagt tccatacggc ctggtggcgc tcgctgatgc
     1741 cgccgcggcg catttccaat ggcagggccg cgtagaacgt cagcggccgc tcttcgaaac
     1801 ccgggggcag gaacatgcgc gcgcagggga acgggggcgc cagatattct tccaccaacc
     1861 agcggacgag ggccacctgc tccggcgaaa gcaacggctc cggctcgatc aagccctcga
     1921 tcgggacgat acgctccggg tcgaagcgcg gcgcgtcgcc gagatcgagt acgacaccct
     1981 ggaggcgccg tcgaccgaag ggcacataca cggcatggcc ggctctcaca tcgagacctt
     2041 cgggaacggc gtaggtgtac gtgtcgcggt gcgggaagtg cgcgttcacg gcaacttccg
     2101 cgaaccgggt accggcggcg aggtgtgaca tggatgtctc ggcggcctgg tcgtgtgaac
     2161 tgccggcagc cgcgctgggc ttaggtgcgg cccatctcct tgatgcgggc tttcttcccg
     2221 gtgcgaccgc gcagatagta gagcgccttg cgccgcacct tgccacggcg cagcacctcg
     2281 accttgtcta tccggggcga atgggtgagg aacgtgcgct cgacacccac actgtgcgag
     2341 gcgacgcgac gaacggtgaa gttggagctt gcaccggccc tgcgcatgcg gatcaccacg
     2401 ccctcgaacg cctgaacgcg ttcccggtcg ccctcgacaa ctttgacatg aacgcgaacg
     2461 gtatcgcccg ggccgaattc gggcacgttg gcgttcgggc gatagtcgat gagactcgtt
     2521 gttgcggtaa ggtcaggcat gcaagtcctc gcaggcgtag tcagtctact atgaagcggc
     2581 gcgtcacgcg ggtcaactct tgtccgatgc cggctcggag agagcctcct ctgcctgacg
     2641 gcgctcctcc tcgctgaggc gagcttgcgc cagcaggtcg gggcggcgct gcgctgtgcg
     2701 gaggatgcgc tgccatcgcc gccacttggc cacctcggcg tgatggccgg acagcaggat
     2761 gtccggcacc ctcatgccac ggaactcggg cggccgcgtg tactgcggat actccagcaa
//
LOCUS       DATMBB010000360         3382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_58523, whole genome shotgun sequence.
ACCESSION   DATMBB010000360 DATMBB010000000
VERSION     DATMBB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58523"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..2384)
                     /locus_tag="VNL92_07685"
     CDS             complement(174..2384)
                     /locus_tag="VNL92_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HWO94630.1"
                     /translation="MIWLAPITAIVAASIAVPALLILYFLKLRRRDVEVSSTLLWKKA
                     IQDLQANAPFQKLRRNILLLLQLLALSAMLLALGQPQITRQAVGGTRHVILIDRSASM
                     KSLDEDDARGGMQSRLDAARKQALALVDSLREGGLMDRDGGDEAMVIAFAAQGEVLQP
                     MTRDKAALRRAIEAITPTDAPTSIEEAMRLALAQRPRRVVENVGLDAGPPITFHIFSD
                     GRIPDAEKAVPGPEDSVVFHRLGKPDASNVGIVGMRAERSFENPARLSVFVSLMNTST
                     TSRLVDVELLVDGAGAGIKTTTIPGAKAELLSAVESARAEAAEREMRETVGTSGSQQP
                     AAPTRFLPGIGGVVFSLDHAPGATVQVRLRDPASGGPISLDVLPVDDSGWLVVPAARR
                     LAVAVVSTKGNLFLSAALAGLPLAKLSEMSPDTFRKRVRDGTMGEYDVVILDGWLPDG
                     AKAAPVADTAQPGPPPTEQLPPGRYLVLGSVPLRLGESPSGLKDLGERTGARIVDWRR
                     DHPLLRAVNLDALEVSKGRALGVEGGAGAVVLAVGDDGPMIVEVSSADTRCIVVPFDI
                     AESTWPFDVSFVVFLASATTYLSDDAGTGASGRMVQPGSVLSDRLPMPASGVDIRLPD
                     GTSQKIEPGVDGRIVFGPLTTVGVHEVSWSGQPGANDLPDGSRAKRLYAANLFEPRES
                     EVGSTDRVELASRVVERSGEQATQANRRLWPWLLAGALVVILLEWFVYNRKVQV"
     gene            complement(2633..>3382)
                     /locus_tag="VNL92_07690"
     CDS             complement(2633..>3382)
                     /locus_tag="VNL92_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWO94631.1"
                     /translation="DHRPYAIGDDIRHIDWNIYARLDALFLKLFLEEEDLSLQLVLDA
                     SESSDCGEPNKFFFMQKAVMALGYIGLVNLNRVSAIAMGPGGIVSAIRDLRGRRRVHD
                     LARWVCSLEPMGTFSFKSAAERIALTRRGKGLMLVFSDFFFKEGFEEGLRRLVGHGYD
                     VYAVQVLSPQEVEPSLTGDLRLKDVEDADLAEVTISAPLLKRYKANLAAYCEHMRTFC
                     ARRDMTHMVVRSDTPIDALVLDQLRRRGVVK"
BASE COUNT          614 a         1158 c         1077 g          533 t
ORIGIN      
        1 gtgcggaggt acggcgcgcc ggtggaggtg gagaaggagg aagtcgagcg cgggcggtat
       61 cagcatcccg agttgtacga ccagccgaga gaacgcggca tgattcctgc cgctgaggaa
      121 ctccagcata acgtggcgcg agcagtccct cccgaggaca caccgtaagt tgattacacc
      181 tgcaccttgc ggttgtagac gaaccactcc agcagaatca caacgagcgc gccagcgaga
      241 agccacggcc agagccgccg gttcgcctgc gtggcctgct ccccgctccg ttcgacgacg
      301 cggctggcca gttccacgcg atccgtcgaa ccgacctccg actcgcgcgg ctcgaagagg
      361 ttggccgcgt acaaccgctt cgcgcgcgaa ccatccggca ggtcgttcgc gcccggctgc
      421 ccggaccacg agacttcatg cacacccacc gtggtcaaag gcccgaagac gatccgccca
      481 tccacgcccg gttcaatctt ctgcgacgtg ccgtcgggca gtcgaatatc aacacccgaa
      541 gccggcatcg gcagtcggtc ggagagcacg gagccgggtt ggaccatccg cccgctggcg
      601 cctgttcccg catcgtcaga gaggtacgtc gtcgccgacg cgaggaacac cacgaacgac
      661 acatcgaacg gccacgtgct ctccgcgatg tcgaatggga cgacgatgca ccgtgtgtcg
      721 gccgacgaaa cctcgacgat catcggcccg tcgtcaccaa cggccagcac gaccgcaccg
      781 gctccgcctt ccaccccgag cgcccgcccc ttggagacct ccagcgcgtc gagattcacc
      841 gcccgcagca agggatgatc gcgccgccag tccacgatac gggcaccggt ccgctcacca
      901 agatccttca gccccgatgg actctcgcca agccgcagcg gcacgctccc gagaacaaga
      961 tatcgcccag gcggcaactg ctccgtcggc ggcgggccgg gctgcgccgt gtcggccacc
     1021 ggggctgcct tcgcaccatc cgggagccac ccgtcgagga tgacgacgtc atactccccc
     1081 atcgtcccgt cacgaacgcg cttgcggaac gtgtcgggcg acatctcgga gagttttgcc
     1141 aggggcaggc cggccagcgc ggcgctgagg aacaggttgc ccttcgtcga gaccaccgcc
     1201 acggcgaggc gcctggccgc cggcaccaca agccatcccg agtcatcgac cgggagtaca
     1261 tcgagggaga tcggtccgcc cgaggccgga tctcgcaatc tcacctggac ggtcgcgccg
     1321 ggagcatggt ccagcgaaaa gacaactccg ccaatccccg gaaggaatcg cgtcggggcg
     1381 gcgggttgct gactcccgct cgtgccgacc gtctcgcgca tctcccgctc ggccgcttcg
     1441 gcccgggcgc tttcgaccgc cgacagcagt tccgccttgg cgccggggat cgtcgtcgtc
     1501 ttgatccctg cgcccgcgcc gtccacgagc agttcgacgt ccacgaggcg cgacgtggtg
     1561 ctcgtgttca tgagcgacac gaagacgctc agacgcgccg ggttctcgaa cgaccgctcc
     1621 gcacgcatcc ccacgatccc gacgttgctc gcgtccggct tgcccagcct gtggaacacc
     1681 accgaatcct ccggcccggg cacggccttc tcggcgtcgg gaatccgacc atcggagaaa
     1741 atgtggaacg tgattggcgg cccggcgtcg agacccacgt tctcgaccac gcgccgcggg
     1801 cgctgcgcca gcgcgaggcg catcgcttcc tcgatcgatg tcggcgcgtc cgtcggcgtg
     1861 atggcctcga tggcgcgacg aagagcggcc ttgtcgcggg tcatcggctg gagaacctcc
     1921 ccctgcgcgg cgaaagcaat gaccatcgcc tcgtctccac cgtcgcggtc catcagcccg
     1981 ccctcgcgaa gcgaatcaac cagcgcaagc gcctgtttcc tggcagcgtc gagccgggac
     2041 tgcattccac ctcgcgcgtc gtcctcgtca agggacttca tggaagcgga gcggtcgatg
     2101 aggatgacat ggcgcgtgcc gccgacggcc tggcgcgtga tctgcggctg gccgagcgcc
     2161 agcagcatcg cggaaagggc gaggagttga agaaggagca ggatgttgcg ccggagtttc
     2221 tggaaggggg cattggcctg aaggtcctgg atggccttct tccacaggag cgtggaggag
     2281 acctcgacat cccgccggcg cagtttcagg aagtacagga tgagcagcgc cggcacggcg
     2341 atgctcgccg caacgatggc cgtgatgggt gcgagccaga tcatggggta tgtccggcga
     2401 cacggggaca cggtgacacg gcgacatggt ggcggggcaa gcggctcatc ttgctgtttc
     2461 cctgcgttcg aatgagcgga tcgggcctcc aggaaccacc gatgcacgaa ccacgtggat
     2521 cgcgtgcgcg agttccatcc cccactgcca cgcaacacgg cctcgatgcg atggaattcc
     2581 gttctttctc actggatcgc tccgtccctg tgtcaccgtg tcgctccact cctcacttca
     2641 ccactccccg cctccgcaac tggtccagca caagcgcatc gatcggagta tcggagcgca
     2701 cgaccatgtg cgtcatgtcg cgacgggcac agaatgtccg catgtgctcg cagtacgcgg
     2761 caaggttcgc cttgtagcgc ttcagcagcg gcgcagaaat cgtcacctcc gccaggtccg
     2821 cgtcttccac gtccttcagg cgcaaatcgc cggtcaggga cggctcaacc tcctgcggcg
     2881 aaagcacctg tacggcgtac acgtcgtagc cgtgaccgac caggcgccgg agcccctcct
     2941 cgaatccctc cttgaagaag aaatcggaga agacgagcat caaccccttg ccgcgccggg
     3001 tgagcgcgat tcgctcggcc gcggacttga atgagaacgt ccccatcggc tcaagcgagc
     3061 acacccagcg cgccagatca tgcacgcgac gccgcccgcg caggtcgcgg atggcggaca
     3121 cgatgccgcc cggacccatc gcgatggcgc tgacgcggtt gagattcacc aggccgatgt
     3181 agccgagggc catgacggcc ttctgcatga agaagaactt gttcggttcg ccgcagtcgc
     3241 tgctttcgga ggcgtcgagg accagttgga gcgagagatc ttcttcttcg aggaagagtt
     3301 tgaggaagag tgcatcgagg cgcgcgtaga tgttccagtc gatgtgccgg atgtcgtcgc
     3361 cgatggcgta ggggcggtgg tc
//
LOCUS       DATMBB010000361        10428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_216677, whole genome shotgun sequence.
ACCESSION   DATMBB010000361 DATMBB010000000
VERSION     DATMBB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 10428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10428
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216677"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..3767
                     /gene="rpoC"
                     /locus_tag="VNL92_07695"
     CDS             6..3767
                     /gene="rpoC"
                     /locus_tag="VNL92_07695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWO94632.1"
                     /translation="MALGERVIVEKRKAIDAAAIERLRAAAQDAVASLEQEAVQRRSD
                     VQLEADSAVQALHHQFEVDAEPHREALRQAREAIRKQYQTDIEELEDLRPLQLLTDAR
                     YRELEQKYGHVFKAGMGAEAVLQILQDTDLDMLREQLGQEIHSTSGQRRKKATKRLRV
                     VEALRKSGNRPEWMIFSVLPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKR
                     LLDLGAPEIIIRNEKRMLQEAVDSLIDNGRRGRAVSGQGNHKLKSLSDLLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPELKLHQCGLPKRMALELFKPFVMHALVAQGLAHNIKSA
                     KRIVERARPEVWDVLDEVIKERPVLLNRAPTLHRLGIQAFEAVLIEGSAIQLHPLVCH
                     AFNADFDGDQMAVHVPLSREAVAEARQVMLATYNLLSPSSGDPVVAPTLDMVLGCYYL
                     TTSKPGARGEYRPAENGNGPEGVYGSFAEAVLAYELGVLELQANIRVRDERTQNEFIE
                     TTVGRIIFNEALPAEMGFRNQVMDKRNLKDVVAECHERFGNEVTAKAVDAIKGVGFRF
                     ATQSGTTIAMNDIKVPEAKGELLREADEKIREVEEQYMLGLVTEQERYDRAIEIWTET
                     TNRIQQLIAENLDRYGSVYMMANSGAKGNIAQIRQMAGMRGLMSDPSGRIIDLPIRAS
                     FREGLPVLEYFISTHGARKGLADTALRTADSGYLTRRLIDVAQDVIILHDDCGTEDGI
                     WIERGADVGVQKTLQERIVGRYAAQPIVDPGTGEVIVGRNEEIQDHQANAIVSAGIER
                     VYVRSPLSCGARRGLCRPCYGRDLARGNLVKPGEAVGIIAAQSIGEPGTQLTMRTFHT
                     GGVAGLDITSGLPRVEELFEARIPKGQAIITEIDGIVEMIRDGDSRIVRVTRNDTYRD
                     DYELPEGYEVAVQPGAVIDSGSVLAQPPAAEEGEERAVDAGPVVARVSGVVEVDGSKV
                     AVLYEERDVREYVIPAASRLHSDLQDGDYVVAGQQLTEGSVNPQDILRVMGRERVQQY
                     LVAEVQRTYELQGVNINDKHIEVVVRQMLRKVRIDAPGDTTMLPGELVDRFAYEELNA
                     KVLAEGGEPATAETVLLGVTKASLNTESFLAAASFQETTRVLTEAAISGAVDHLLGLK
                     ENVIIGKLIPARAEIQLPPRIERPRMPLLPRLDDEDASLAALMDAEGDLDFVEEDMLF
                     DEESEGAPEPEASMHIEDTGEEWEGEVPEAQTAAEAIRTGYVALPDEEE"
     gene            complement(3826..4515)
                     /locus_tag="VNL92_07700"
     CDS             complement(3826..4515)
                     /locus_tag="VNL92_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWO94633.1"
                     /translation="MPRVLVLSSAVLEIRRALQAVEREGFTTILVAPSDDAIRLLASE
                     SPEAVILDLTAAWPTSEPDDLLRHGIPEGCRTLAVIPEAKLPMLRTLPLDDFVLHPPN
                     GEEVLARLTRLLANAIDESAHIIRYSDLLIDTANYTVSLAGHPVELTFKEYELLKFLA
                     SNPGRVFTREVLLNRVWGYDYFGGSRTVDVHIRRLRSKIEDRNHTFVDTVRNVGYRFR
                     ASSSAVAQQSE"
     gene            4654..4728
                     /locus_tag="VNL92_07705"
     tRNA            4654..4728
                     /locus_tag="VNL92_07705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4686..4688,aa:Thr,seq:cgt)
     gene            complement(4792..5700)
                     /locus_tag="VNL92_07710"
     CDS             complement(4792..5700)
                     /locus_tag="VNL92_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94634.1"
                     /translation="MISQTGEMEVTYPRIAQPNLALRSLRSLWRFVRTKPLGAIGGFF
                     VLLLVFSALTAEFISPYDPLTQVPQDRLQGPSWDHWLGTDQLGRDQLSRIIHGSRTSL
                     FVGFIAVGLGTLGGTIVGLVSGYLGGLVDLILQRVMDAFQAFPAIVLALALISVLGTG
                     IENVMLAIGIVIMPGAGRIVRGAVLAQKANLYVEAARTIGCSQTRILVRHILPNVAAP
                     IIVIVSIQLGTAILAEASLTFLGLGTQPPDPSWGLMLSSATQRFMVIQPWMAIAPGIA
                     ISLAVFGFNLFGDAIRDVLDPRLRGT"
     gene            complement(5710..6663)
                     /locus_tag="VNL92_07715"
     CDS             complement(5710..6663)
                     /locus_tag="VNL92_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94635.1"
                     /translation="MGRYILKRLLLTIPTLLAVTFIVFGLIRFIPGDAAMVQLGETGR
                     VGDEEELDRLRHALGLDKPWYEDYAEWIGGVARGDLGESIWSGRDVRGDILDRLPVTL
                     ELAALAVIFSFVIAIPGGIISAARQDSMADYAIRIISILGLSIPAFWIGTLAFVLPPR
                     WWGWSPPVNYTEFLEDPIANLQQFWLPAAILALSLSASTMRITRSSMLEVLRQDYVRT
                     AWAKGLRERSVIVRHALKNAMIPVVTLIGLQFSVLLGGTVIIEQIFSLPGVGRLTLEA
                     ILQRDYPLIQGSVLFIATLFVLINLVVDVSYAWLDPRIRFS"
     gene            complement(6673..8391)
                     /locus_tag="VNL92_07720"
     CDS             complement(6673..8391)
                     /locus_tag="VNL92_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94636.1"
                     /translation="MEDTVSRYWANVRRRTTRRRLLTSGAAAGAGAASLALVGCGDDD
                     DDDDDDGAATATTAPGTGTAGPSGEARPGGSVVIAASSPPHLDPTLNASFATVAMHRN
                     IYNRLFRTVSGPGEDPGSLRMIPDLAEALPEQPDETTYIIKVRQDVTFHNMPPVNGRQ
                     LTGEDVVYTFQRLLDPNVASPNRSLYTRINNIELMDPYTVRITTDEPYSPLIVYLGHY
                     YSGWIIPREAVEQFGDLRGNAIGTGPFTLESFSPNQEAVFAKNPNFFQKPYPYIDRLQ
                     YLFIDQAQPRIAALEAGNIDVGTFPAQDLERLEGLLTDDGGGVIRTLPGLASSVIMMT
                     KRAPLDDERVRRALSLLLDQQLMIDVTLFGEGVFAGLGMPPLYSEWSLSQDELRELHP
                     YDPEQAQALLEEAGMGNGFDTNLYFSPFYSAAGVPVEDFVEIYVESLRQAGINAEVEA
                     KEYQAHLQTWTTENFSLYLGPRTIGLTPDEFYAAGFTPGGARNFSKLDDPEITRMSEQ
                     IQRTFDVDEQIDLSHQLMRRLGELAPEAPLPSPYAFTGYRGRLKDFSVSADFGSPTYQ
                     WTWVDE"
     gene            8618..9322
                     /locus_tag="VNL92_07725"
     CDS             8618..9322
                     /locus_tag="VNL92_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94637.1"
                     /translation="MVSKLRDRIRRLGRREAGPMGFAPAVAAKQPGMLLILRLPAKGA
                     VEPALEGGADAVLVDAGGRSVTKQPPDLLLGLDVRGAADDVNLEGADFIVADGAAPSA
                     VLANEGRGLVLAVDEAWPDALLRASETLAPDALFVELGAQPTLQRILELRRVALVGGA
                     PLLLGIAGSIDPPILRVLRDAGVVGLVVDSKGASAMPQLRETINSMPPRRRPNEADSP
                     LLPHVEVAHEHEDEMD"
     gene            9402..9878
                     /locus_tag="VNL92_07730"
     CDS             9402..9878
                     /locus_tag="VNL92_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWO94638.1"
                     /translation="MTSEKRDAYLQIGEVAERTGVTQRTLRFYEEKGLLPAPTRMEGG
                     FRLYSEDDIRRVEQIRRLQGLLGFSLAEIKEMVEAEEERLQLRETARQLPNDIERRRQ
                     ATLRAIEVTERQRDVIRQKVQQLLEMEHSIEEKLAKYRSWLDELEREDGVSAEAAR"
     gene            complement(9875..10195)
                     /locus_tag="VNL92_07735"
     CDS             complement(9875..10195)
                     /locus_tag="VNL92_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94639.1"
                     /translation="MIRCAPRQLGLAASLVIAIAAAWVLMHHTAQAADVANTHESRVL
                     FQDEATSDENDDLVPVALGTAGVAAAGGLAAGVGYLYRRRSGMAARYDDGFTPSDPRD
                     RPVH"
BASE COUNT         2002 a         3071 c         3537 g         1818 t
ORIGIN      
        1 aaccgatagc gctcggcgag cgtgtcatcg tcgagaagcg gaaggccatc gatgcggcag
       61 ccatcgagcg cctgcgggct gcagcacaag atgccgtcgc ttcattggag caggaggccg
      121 tgcagcgacg cagcgacgtt cagctagagg ccgactctgc cgtgcaggcg ctgcaccatc
      181 agttcgaagt tgatgcggag ccgcaccgcg aggcgctgag gcaagcgcgt gaggcaatcc
      241 gtaagcagta ccagacggac atcgaagagt tggaggacct acggcccttg cagctcctca
      301 ccgatgcccg ttaccgcgaa ctcgagcaga agtacggtca cgtgttcaag gccggcatgg
      361 gcgccgaagc ggtgctccaa atcctgcaag acactgacct cgatatgcta cgggagcagc
      421 taggccagga gatacactcg acgagcgggc agcgccggaa gaaggcgacg aagcggcttc
      481 gcgtggttga ggcgctgcgt aagtctggga accggcccga gtggatgatc ttctcggtgt
      541 tgccggtcct cccgcccgac ctgcgcccga tggttcagct cgatggcggt cgcttcgcta
      601 cgagcgacct aaacgacctg tatcgccgcg tcatcaaccg caacaaccgg ttgaagcggc
      661 tcctcgacct cggggcgccc gagatcatca ttcgcaacga gaagcgcatg ctgcaggaag
      721 cggtggactc gctcatcgac aacgggcgtc gcggacgagc tgtctccgga cagggcaacc
      781 acaagctgaa gtcgctctcc gacttactga agggcaagca gggccgcttc cgtcagaact
      841 tgctgggcaa gcgcgtcgac tattcgggcc gttcggtcat cgtcgttgga cccgagctca
      901 agctgcacca gtgcggcttg ccgaagcgca tggcgctcga gctgttcaag ccgttcgtca
      961 tgcacgcact cgtggcccaa gggttggctc acaacatcaa gagcgccaag cgcatcgtcg
     1021 agcgtgcccg tcccgaggtg tgggacgtgc tcgatgaggt cattaaggaa cggccggtgc
     1081 tgttgaaccg cgccccgacg ctgcaccgcc tcggcatcca ggccttcgag gcggtgctca
     1141 tcgagggtag cgcgattcag ctgcatccgc tcgtgtgcca cgccttcaac gctgacttcg
     1201 atggcgatca gatggcagtc cacgtacctt tgtcgcgtga ggctgtcgcc gaggcccgac
     1261 aggtgatgct cgccacgtac aacctgctct cgcccagttc gggggatccg gtcgtcgccc
     1321 cgacgcttga catggtattg ggctgctact acctgacgac ttccaagccg ggagcgcgtg
     1381 gtgagtaccg tcccgcagag aacggaaacg gtccggaagg ggtatatggc tcgttcgccg
     1441 aggcggtcct cgcctacgag ctcggcgtgc tcgagttgca ggccaacatt cgcgtgcggg
     1501 atgagcggac gcaaaacgag tttatcgaga caacggtggg ccggatcatc tttaacgaag
     1561 ctctaccggc cgagatgggc ttccggaacc aggtgatgga caagcgcaac ctgaaggacg
     1621 tcgtggccga atgccatgaa cgcttcggca atgaggttac ggccaaggca gtcgacgcca
     1681 tcaagggagt gggcttccga tttgcaaccc agtccggtac cacgatcgcc atgaatgaca
     1741 tcaaggtgcc ggaggcgaag ggcgaactcc tccgagaagc cgacgaaaag atccgtgagg
     1801 tcgaagagca gtacatgctg ggtctcgtca ccgagcaaga gcgttatgac cgtgccatcg
     1861 agatttggac ggagacgacg aataggatcc agcagctcat cgcggagaac ctcgatcgct
     1921 atggctccgt ctacatgatg gccaacagtg gtgcgaaggg gaacatcgct caaatacggc
     1981 agatggcggg tatgcgagga ctcatgtcgg acccatcggg tcgcatcatc gacctgccga
     2041 tccgcgccag tttccgcgaa ggcctgcccg tcctcgagta cttcatctcg acgcacggcg
     2101 cgcgcaaggg gttggcggac acagccctgc gtaccgccga ctctggatac cttacccggc
     2161 ggctcatcga tgtggcgcag gacgtcatca tcctgcacga cgactgtggg accgaggatg
     2221 gcatctggat cgaacggggc gcggacgtgg gcgtgcagaa gacactgcaa gagcgcattg
     2281 tcggccgcta cgcggcgcaa ccgatcgtcg atccgggcac aggcgaggtg atcgtcgggc
     2341 ggaacgagga gattcaagac caccaggcaa atgcgatcgt atcggctggg atagagcgcg
     2401 tgtacgtccg ttcgcctctc agctgcggcg ccaggcgcgg actctgccgg ccgtgctacg
     2461 gtcgcgatct cgcgcgcggc aatctcgtga aaccgggcga agccgtgggt atcatcgcgg
     2521 ctcagagcat tggtgagcct ggaacccagc tcacgatgcg gacgttccac acaggcggtg
     2581 tagccggcct cgatatcacg tccggacttc ctcgggttga ggagctgttc gaggcgcgca
     2641 tccccaaggg gcaggcgatc atcaccgaga tcgatgggat cgtcgagatg atccgcgatg
     2701 gggattcccg aatcgttcgc gttacgcgga acgatacgta ccgcgacgac tacgagcttc
     2761 ctgaaggata cgaggtggca gtccagccgg gcgcggtcat cgattccggc agtgtgctcg
     2821 ctcagccgcc cgccgcggag gagggcgagg aacgagccgt cgacgccggc ccagtggtcg
     2881 cccgcgtttc gggcgtcgtt gaggtcgacg gttcgaaggt ggccgttctg tatgaggagc
     2941 gggatgttcg cgagtacgtc atccccgctg cctcgcgtct gcacagcgac ctgcaggacg
     3001 gcgattacgt ggtcgcggga cagcagctta cggaaggatc cgtgaatccg caggatatcc
     3061 tacgggttat gggccgcgag cgtgtccagc agtacctcgt cgccgaggtt cagcggacgt
     3121 acgagctgca gggcgtcaac atcaatgaca agcacatcga ggtggtcgtt cgccagatgc
     3181 tgcgcaaggt ccgcatcgat gctccgggtg acacgacgat gctgccggga gagctcgtgg
     3241 accgctttgc gtacgaggag ttgaacgcga aggtcctggc tgagggaggc gaaccggcga
     3301 cggccgaaac cgtgctcctc ggcgtgacca aggcatcgct gaacacggag agcttcctcg
     3361 cggcagcctc gttccaggag acgacgcgag tcctcaccga ggcggccatc agtggtgctg
     3421 tcgaccacct gcttggcttg aaggagaacg tcatcatcgg caagctgatt cccgctcgcg
     3481 ccgagataca gctgcccccg cgcatcgaac gtccgcggat gcctctacta ccgcgcctcg
     3541 acgacgagga tgcttcgctc gctgcactca tggatgcgga gggggacctg gacttcgtgg
     3601 aggaggatat gctcttcgac gaggagtcgg aaggcgcgcc ggaacccgaa gcctcgatgc
     3661 acatcgagga taccggcgaa gaatgggaag gcgaggtgcc ggaggcgcaa acggccgcgg
     3721 aggccattcg gacgggatac gtcgcgctgc ccgacgaaga ggaatagctt ccgtctctca
     3781 cgccattcga gagcagtcga tagcccgtct cgccgaagag gcgggctatt cgctttgttg
     3841 ggccacagcg ctggagctgg cacggaagcg atagccgacg ttacgcacgg tatcgacgaa
     3901 cgtatggttg cgatcttcta tcttgctgcg cagccgccgg atgtgcacgt cgaccgtccg
     3961 cgagccgccg aagtagtcgt atccccagac gcgattgagc aggacctcgc gcgtgaagac
     4021 gcgccctggg ttcgatgcga ggaacttcag gagttcgtac tccttgaacg tgagttcgac
     4081 agggtggccg gcgagggaca cggtgtagtt cgccgtatcg atgagaagat cggagtatcg
     4141 aatgatgtgg gccgattcgt cgatggcgtt ggcgagcagc ctcgtcaggc gcgcgagcac
     4201 ttcttcgccg ttgggagggt ggagcacaaa gtcatccagc ggcaaggtgc gtagcatagg
     4261 cagcttcgcc tcggggatga cagccagggt acggcaaccc tcgggaatgc cgtgtcgaag
     4321 taagtcgtct ggttcgctgg ttggccaagc ggcggtcaga tcgagaatga cggcctcggg
     4381 cgattcggag gcgaggaggc ggatagcgtc gtcgctgggt gcgacgagga tggtggtgaa
     4441 cccctcgcgc tccacggcct gcaacgcgcg tcgtatctcg agcacggcgc tactgaggac
     4501 caacacgcga ggcaatgggc cgctcctgct ggggttgatg tgcgatagcg taggacgcgc
     4561 ggcaggaagg cgcaaggctg agtgtttgag ggttgcgccc gtggtcacct ataatgttgc
     4621 cgtgttcccc tcacgtccga tggagcactg cgcgccagcg tagctcagtg gtagagcaac
     4681 ggtttcgtaa accgtaggtc aagggttcga atcccttcgc tggctccagt ccaggacgaa
     4741 agcgatgcgg ccatgacggc cgcatcgctg cgttcgacaa tggcgctggc actatgtccc
     4801 gcggaggcga gggtcgagca cgtcgcggat ggcatcgccg aagaggttga agccgaacac
     4861 ggccaagctg atggctatgc cgggggcgat agccatccac ggctgaatga ccatgaagcg
     4921 ctgggtggcg ctgctcagca tgagccccca cgagggatcg ggcggctgcg tgcccagccc
     4981 caggaacgtc agcgaagcct cggccaagat ggcggtcccc aactggatgc tcacgatcac
     5041 gataatcggc gcggccacgt tagggagaat gtgacgaacg aggatgcgcg tctgcgagca
     5101 gccgattgtc cgcgccgctt cgacgtacaa gttcgccttc tgggcgagca cggctccgcg
     5161 cacgatgcgc cccgcacccg gcatgatcac gatgccaatg gcgagcatta cgttctcgat
     5221 gccggtgccc agcaccgaga tgagcgccag tgccagcacg atggccggga acgcctggaa
     5281 ggcgtccatg acccgctgca ggatcaggtc gacgaggccc ccgaggtacc cggagaccaa
     5341 gccaacgatg gtgccgccca gcgtccccag cccgacagcg atgaatccga cgaacagcga
     5401 ggtgcgagag ccatggatga tacgactcag ctggtctcgc ccgagctggt cggtgccgag
     5461 ccagtggtcc cacgagggcc cttgtagccg gtcctgcggc acctgagtca gcggatcgta
     5521 gggcgagatg aactcggcgg tgagcgcgga gaagacgagc agcagtacaa agaagccgcc
     5581 gattgccccc agcggcttcg tgcggacgaa tcgccagagc gagcgtaggc tgcggagggc
     5641 gaggttcggc tgcgctattc gcggatacgt gacctccatc tcgcccgtct gcgatatcac
     5701 cacgtcctcc tacgagaagc ggatgcgcgg atcgagccac gcgtacgaga cgtcgacgac
     5761 caggttgatc aggacgaaga gcgtggcgat aaagagcacg gaaccctgga tgagcgggta
     5821 atcgcgctgg agtatcgcct cgagcgtcag ccgtcccacg cccggcaggg agaagatctg
     5881 ctcgataatc accgtcccgc ccagaaggac gctgaactgc aagccgatca gcgtcaccac
     5941 gggaatcatt gcgttcttga gcgcgtggcg aacgatgacg gagcgttccc gaagcccctt
     6001 cgcccacgcc gttcgcacgt agtcctgccg cagcacttcc agcatggacg accgggtgat
     6061 gcgcatcgtg ctcgccgaaa ggctaagggc gaggatcgct gcaggcagcc agaactgctg
     6121 caggttggcg atgggatcct ccaggaactc ggtgtagttc accggcggcg accaccccca
     6181 ccagcgtggt ggcagcacga acgcgagggt tccgatccag aatgcgggaa tgctcaaccc
     6241 caggatcgag atgatgcgga tggcataatc ggccatcgaa tcctgacggg ccgccgagat
     6301 gatgccgcct ggtatggcga tgacgaacga gaagatgacg gccagcgcag ctagttcgag
     6361 cgtcactggc agccggtcga ggatgtcacc gcggacatcg cggccggacc agatcgattc
     6421 gccgagatcg ccgcgtgcta cccctccgat ccactccgcg taatcttcgt accacggttt
     6481 gtcgaggccg agcgcgtgcc gcaggcgatc gagctcctcc tcgtctccaa ccctccctgt
     6541 ctcgcccaac tgcaccatcg ctgcgtcgcc gggaatgaag cgaatgagcc cgaacacgat
     6601 gaacgtgacg gccagcaagg tcgggatcgt gagcaggaga cgtttgagga tgtagcgtcc
     6661 catgagcgct cgctactcgt cgacccatgt ccattgatag gttggtgagc cgaaatctgc
     6721 ggagaccgag aagtccttga gcctcccgcg gtagccggtg aaggcataag ggctgggcaa
     6781 cggcgcttcg ggcgcgagct ccccgaggcg gcgcatcagc tggtgggaaa ggtcgatctg
     6841 ctcatccaca tcgaacgtgc gttgaatctg ctccgacatg cgggttatct cgggatcgtc
     6901 gagcttcgag aagttgcgcg cgccgcccgg ggtaaatccg gccgcgtaga actcgtccgg
     6961 cgtcaggccg atggttctcg ggcccaggta gaggctgaag ttctcggtgg tccaggtttg
     7021 gaggtgagct tggtactcct ttgcctccac ctccgcattg atgcccgctt gacgcaggga
     7081 ttccacgtag atctcgacga agtcttcgac agggacgccg gcggcggagt agaagggcga
     7141 gaagtagagg ttcgtgtcga agccattccc catgccggct tcctcgagca gcgcctgggc
     7201 ttgctctgga tcgtacgggt ggagctctct cagctcgtcc tgggaaaggc tccactcgct
     7261 atagaggggt ggcatgccaa gcccggcgaa cacaccctcg ccgaacaacg tcacgtcaat
     7321 catgagttgc tggtcgagca gcaacgatag cgcccgacgc acccgctcgt cgtcgagggg
     7381 cgcacgcttg gtcatcatga ttactgagct cgccaggccg gggagggtgc ggataacgcc
     7441 tccaccgtcg tcggtcagga gcccctcgag gcgctcgaga tcctgggcgg ggaacgtgcc
     7501 cacgtcgatg ttccccgctt cgagtgcggc gattcgcggc tgcgcctggt cgatgaagag
     7561 atactgaagc cgatcgatgt aagggtacgg cttctggaag aagttggggt tcttggcgaa
     7621 gacagcctct tgattcggcg agaagctttc gagcgtaaag gggccggtgc cgatcgcgtt
     7681 gccgcgcagg tcgccgaact gctccacagc ctcacgcgga atgatccatc cggagtagta
     7741 gtggcccaag tacacgatga ggggcgagta cggctcgtct gtcgtgattc gcacggtgta
     7801 cgggtccatg agctcgatgt tgttgatacg agtgtagagc gagcgattcg gagaggcgac
     7861 gttcgggtcg agcaggcgtt ggaacgtgta gacgacgtcc tcgcccgtaa gttgccgtcc
     7921 attgacaggc ggcatgttgt ggaacgtcac atcctgccgc accttgatga tgtaggtcgt
     7981 ctcatcaggc tgctccggca gcgcttcagc gagatcgggg atcatacgca ggctgcccgg
     8041 gtcttcgccc ggacccgaga cggtgcggaa gaggcggttg tagatgttgc ggtgcatggc
     8101 aacggtggcg aaggaggcgt tgagcgttgg gtcgaggtgg ggtgggctac tggccgcaat
     8161 gacgaccgag cctcccggcc tcgcctctcc tgagggtccg gcagtgccag ttcccggagc
     8221 ggtcgtcgcg gtggcggctc catcgtcatc gtcgtcgtca tcgtcatcgc cacagccgac
     8281 cagggcgaga gaggccgcgc cagcacctgc ggccgctccg ctcgtcagaa ggcggcgccg
     8341 agtggtgcgc cgccgcacgt tcgcccaata tcgactgaca gtgtcttcca cggttccccc
     8401 tcctgatgat tgctggcccc gccagcagca tttgcgctgc gatacatgag acaacacggc
     8461 caagtgggag caggtgcgtg gccgcgattg actgtatgta gcaaagacct tcgtaaggtg
     8521 tcaaggtcat cgtccatcag cgtgtgtcgc cacgacggcg tttgacgtac ggtcgagagc
     8581 caacctacgc tacccgatga caggcatgtg aggtgcgatg gtgagcaaac tgcgggacag
     8641 gattcggcgg ctcgggcgcc gcgaggcggg accgatgggg ttcgcgccgg cggttgctgc
     8701 caagcagccc gggatgctgc tcatccttcg tctgccggcg aagggcgctg tcgagccggc
     8761 gctcgagggc ggggcggatg ccgttctcgt cgatgctggt gggcggagcg tcacgaaaca
     8821 gcctccggac ctgctgttgg ggcttgacgt gcgcggcgcg gcggatgacg tcaacctcga
     8881 gggggccgac ttcatcgtcg cggatggggc cgcaccttcg gctgtactgg cgaacgaagg
     8941 acgtggcctc gtgctggccg tggacgaggc gtggcccgac gcactgctcc gagcgagcga
     9001 gacgcttgcg cccgacgccc ttttcgtgga gttgggtgcg caaccgacgc tacagcgaat
     9061 cctcgagtta cggcgtgtcg ctctcgtcgg cggagcgcct ttgctgctcg gcatcgcggg
     9121 cagcatcgat ccgcccattc tgcgagtcct gcgggatgcc ggggttgtcg ggttggttgt
     9181 cgattcgaag ggcgccagcg ccatgcctca gctgcgtgag accatcaact cgatgccgcc
     9241 taggcgacgt cccaatgagg ccgatagtcc gctccttcct catgtcgagg tggcgcacga
     9301 gcacgaggat gagatggatt gagtccgaga ttgctgcggt gaggccgaat gatatacttg
     9361 gcttggagct tgggcgaggc tcggcgagcg gagggatagg gttgacatcg gaaaagcggg
     9421 atgcctacct gcagatcggc gaagtggcgg aacgtacggg cgtgacccag cgcacgctgc
     9481 gcttctatga ggagaagggg ctgctacctg ctcccacgcg tatggagggc ggctttcgtc
     9541 tttattccga ggacgatatc cgccgcgtcg agcagattcg ccgcctgcag ggactcctag
     9601 gcttcagcct cgccgaaatc aaggagatgg ttgaggccga ggaggagcgc ctccagcttc
     9661 gcgagacggc gcggcaactc ccgaacgata tcgagcgccg gcgccaagcc acgttgcgcg
     9721 caatcgaggt caccgagcgg cagcgcgacg tcatccggca gaaggtacag caactcctcg
     9781 agatggagca ttccatcgag gagaagctag cgaagtaccg ctcgtggctg gatgagctgg
     9841 agcgggagga cggagtctcg gccgaggcgg cgcgctagtg gaccggccga tccctcgggt
     9901 cgctgggggt gaagccgtcg tcgtagcgag cggccatacc gcttcggcgg cgatagaggt
     9961 atccgacgcc cgctgctagt cccccggcag cggccacccc tgccgtcccg agggccacgg
    10021 gaaccaagtc gtcattctca tcgctggtcg cctcgtcctg gaagagcaca cgcgattcgt
    10081 gtgtgttcgc tacgtccgcg gcttgtgcgg tatgatgcat caagacccat gcagccgcga
    10141 tggctatcac gagcgaggcg gccaatccaa gctgccgcgg tgcgcatcga atcaaggttc
    10201 tctcctgtgt ttcggaaatg tcgacagaca ttgtagcaga ccggcgttga tgggataggt
    10261 tcgggaaagg aatcgtgacg aatgacgatt gtgcgaagac cggaagctga ggtaggtgag
    10321 cggcctggcc aagcggcggc gcctcctgag ccggctgctc gcccggacga tgatctcgat
    10381 atctattcaa tacagggcga atctgtagac gaggagacgg cgcggcgc
//
LOCUS       DATMBB010000362         2752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_535290, whole genome shotgun sequence.
ACCESSION   DATMBB010000362 DATMBB010000000
VERSION     DATMBB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535290"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1704)
                     /gene="argS"
                     /locus_tag="VNL92_07740"
     CDS             complement(40..1704)
                     /gene="argS"
                     /locus_tag="VNL92_07740"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015407166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWO94640.1"
                     /translation="MIKHEIAALVREAIASAQRSGSLAEVPMPDVVIERPQRPEHGDY
                     ATSVPLRLARAARLAPLAIAEAVVQAMPPHPAIESVEVAPPGFVNVRLAHEWLTLQVE
                     NILAAGDTYGNSEPAIRRRVQIEFVSANPTGPLHVGNGRWATIGSTLANVLEAGGHYV
                     EQEYYINDAGSQVDTFASTLLARYRQLFGKDQPIPENGYPGEYVLEVAAQLRDTYGDR
                     FLDAPIEDPPDEVRRFAVERMVARIRDDLAAMGVSYHVWFNERSLYEGEPSAYQRAMA
                     RLREQGFVAEREGAVWFTSSRLGEEKDNVLIRSDGQPTYFASDVAYHYDKFITRRFDD
                     VIDIWGADHQGHVSRLKAAVGALGIDPARLHIILGQLVTLKRGPEVVRISKRSGDIIT
                     LREVIDEVGADACRFFFLSRSADAQMDFDLELAKKQSQDNPVYYVQYAHARIAGVIAH
                     ALERGVDARAGDVTLLTHPAELELIRKMLRLPELLETIAGNLEPHHLPHYAQELATAF
                     HAFYTECRVVSDDAALSAARLKLVQAARIALARTLHLMGVSAPERM"
     gene            complement(1711..2247)
                     /locus_tag="VNL92_07745"
     CDS             complement(1711..2247)
                     /locus_tag="VNL92_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HWO94641.1"
                     /translation="MGLASREASHHTAGVTGEPFTITAQQYDGTLLKRWPGELRDRSE
                     TIVVAHIWAGTRAEAGDGVWKVAWHTTAYLWPGRWYNAFRYDDPYRGLMGYQCHITTP
                     PEIDATSATFVDLGMSLWIPRRGEPEVKEREIFESFAERFSYSPEVRERAEASLAELR
                     SMHERGVFPFLHASAATG"
     gene            complement(2419..>2752)
                     /locus_tag="VNL92_07750"
     CDS             complement(2419..>2752)
                     /locus_tag="VNL92_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWO94642.1"
                     /translation="TYSYIFDWKSPLLGGALGACHTIELPFVFGTYAAPGLSMLVGEG
                     PEVEELSRKTQDAWLAFARHGDPSNESLGRWPAFDPQSRHVMVLSARAHVEQHPWEAE
                     RRAWDGIL"
BASE COUNT          472 a          872 c          928 g          480 t
ORIGIN      
        1 ggctcctcca tcagcactgc ccctcgatga ggtcgtacct cacatgcgct ccggtgccga
       61 tacgcccatg aggtggagcg tccgcgccag ggcgatgcgg gcggcctgca cgagcttgag
      121 ccgcgccgcc gagagcgccg catcatcgga gacgacccgg cactccgtgt agaaggcgtg
      181 gaatgcagtg gccagctcct gggcgtagtg agggaggtga tgcggctcca ggttcccggc
      241 gatggtctcg agcagctctg gaagtcgtag catcttgcgg atcaactcga gctcggccgg
      301 atgcgtgagg agcgtcacgt cccccgctcg cgcgtcaacc ccgcgttcga gagcatgggc
      361 gatgacgccg gcgatccggg cgtgcgcgta ctgtacgtag tacacggggt tgtcctgcga
      421 ttgctttttc gcgagctcca ggtcgaagtc catctgggcg tctgccgatc gagagaggaa
      481 gaagaagcgg caggcatcgg cacccacctc gtcgataacc tcgcgcaggg tgatgatatc
      541 gccgctgcgc ttcgagatac ggaccacctc tgggccgcgc ttcagggtga ccagctggcc
      601 gaggatgatg tgcagacgcg cggggtcgat gcccagcgct ccgaccgcgg ccttgaggcg
      661 ggacacgtgg ccctggtggt ccgcgcccca gatgtcgatg acgtcgtcga accggcgagt
      721 tatgaatttg tcgtaatggt aggcgacgtc tgaggcgaaa taggtcggct gtccgtccga
      781 tcggatgagg acgttgtcct tctcctcgcc gagccggctc gacgtgaacc agacggcgcc
      841 ctcgcgctcg gcgacgaagc cctgttcgcg cagtcgcgcc atagctcgct ggtaggcaga
      901 gggctctccc tcgtagagag agcgttcgtt gaaccacacg tggtacgaca cacccatcgc
      961 tgcgaggtcg tcgcggatgc gtgccaccat gcgctcgacc gcgaaccggc gcacctcatc
     1021 gggcggatcc tcgatgggcg cgtcgaggaa gcgatcaccg tacgtatctc gcagctgagc
     1081 cgcgacctcg aggacgtact cgccggggta gccgttctcg ggaatcggct ggtccttgcc
     1141 aaagagttgg cggtatcggg ccagcagcgt gctggcaaac gtatcgactt gcgagccggc
     1201 gtcgttgatg tagtattcct gctcgacata atggccgcct gcctcgagca cattcgccag
     1261 ggtgctgcca atggttgccc agcggccgtt cccaacatgg agagggcccg tgggattcgc
     1321 gctcacgaac tcgatctgca cacgtcgtcg tatcgccggc tcgctgtttc cgtaggtatc
     1381 gcccgcggcg agaatattct ccacctgtag ggtgagccac tcgtgcgcga ggcgcacgtt
     1441 cacgaacccg ggaggagcga cctccacgct ctcgatagcc gggtggggcg gcatggcctg
     1501 gacgacagcc tcggcgatgg cgagtggggc gaggcgcgcc gcccgggcga ggcgcagcgg
     1561 cacactcgtc gcgtagtcgc cgtgttcggg acgttgagga cgctcgatga cgacatcggg
     1621 catcggcact tcggcgaggg agccggaacg ctgcgcggag gcgatcgcct cgcggacgag
     1681 ggccgcgatc tcgtgcttga tcatctcgcc ctagcccgtc gcagccgagg cgtgaaggaa
     1741 cgggaacacg ccacgctcgt gcatggaacg aagctccgcc agcgacgcct cggcgcgttc
     1801 gcgaacctca ggcgaatacg agaaccgctc cgcgaacgat tcgaagattt cgcgttcctt
     1861 cacttccggc tcgccgcgtc gcgggatcca caggctcatg ccgagatcca cgaaggtggc
     1921 tgacgtggcg tcgatttcgg gcggagtagt gatgtggcac tgatagccca tgagcccgcg
     1981 atacgggtcg tcgtagcgga acgcgttgta ccagcgcccg ggccaaaggt aggctgtcgt
     2041 atgccacgcg accttccaca cgccgtcgcc ggcttccgct cgggtgcccg cccagatatg
     2101 tgcgacgacg atagtttcgc tccggtcacg tagctcgccc ggccagcgtt tcaggagggt
     2161 gccgtcgtac tgctgcgccg tgatcgtgaa tggctcgcct gtcacaccag cagtatggtg
     2221 cgacgcctcg cgcgaggcaa ggcccatcga gcattcgtac ttcctggaga gcagcaaagc
     2281 gggaataggg gtcgaacgcc atgtcaccat cccgcgccat aagaacctga gcgtcgggac
     2341 cttgagcgcc attcttacgg acgtggcctc gcacctgcag atggatcggg acgcgctgac
     2401 acaagagctc tttcggccct agaggatgcc atcccaggcg cggcgctccg cctcccacgg
     2461 gtgttgctcc acgtgggcgc gtgcgctgag taccatgacg tggcggctct gcgggtcgaa
     2521 tgccggccag cggcccagcg attcgttgct ggggtcgccg tgacgggcga aggcgagcca
     2581 ggcgtcctgc gtcttccgcg acagctcctc cacctcggga ccctcgccca cgagcatact
     2641 cagaccgggc gccgcatacg tgccgaagac gaacggaagc tcgatggtat gacaggcccc
     2701 tagcgcccca ccgaggaggg gcgatttcca gtcgaagatg tagctgtaag tt
//
LOCUS       DATMBB010000363         4032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_574075, whole genome shotgun sequence.
ACCESSION   DATMBB010000363 DATMBB010000000
VERSION     DATMBB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574075"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VNL92_07755"
     CDS             <1..277
                     /locus_tag="VNL92_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94643.1"
                     /translation="QTMGAGPRTVGTSDFFFAACLTYLEEHGLDAEQVEAWEGVSFHA
                     LDPWLRIANELPEGQEIDLGWLEELARVCNEEAVYRRFVEGVRAQEG"
     gene            283..480
                     /locus_tag="VNL92_07760"
     CDS             283..480
                     /locus_tag="VNL92_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94644.1"
                     /translation="MWWQDWLWGFWNGLTAWVVLIVHAFGAWEDFPFYNVGRRGNWYD
                     FGFVLGAGSPFFGALGSRRRG"
     gene            complement(550..834)
                     /locus_tag="VNL92_07765"
     CDS             complement(550..834)
                     /locus_tag="VNL92_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWO94645.1"
                     /translation="MTMKSANDIIPDEATHPGALLRETLESEGISQRELARRMGRPAP
                     AINEIVRGKKRVTAETALELEQVLGIPASIWVNLQARYDLVLATHARAQR"
     gene            833..1180
                     /locus_tag="VNL92_07770"
     CDS             833..1180
                     /locus_tag="VNL92_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94646.1"
                     /translation="MALIVIMVRYLIHSDCPSIGRWCDNEPPAPDKCHRVRSGAITPQ
                     GMKAQRGHPEQKIEGVSRNHELNALQISAGDLASPGAFGLADVLVPSGQLPRREIDTH
                     IVYTLRDNTSRVK"
     gene            1193..2014
                     /locus_tag="VNL92_07775"
     CDS             1193..2014
                     /locus_tag="VNL92_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWO94647.1"
                     /translation="MSDTRVSVFTVDAFADRAFRGNPAAVVPLRQAASRAWMQNVAAE
                     MNLSETAFLEPMAGSAEEPRFGLRWFTPKNEVELCGHATLASAHVLWSEGLASPSAPV
                     VFETLSGPLRCRRLEDGWIEMSFPPEPPLPVEQPHEALLAALRVEPRFIGRSRFDYMI
                     EVPTEDEVRGVTPDLRLLRTVPVRGVCVTAPSADPRYDFVSRFFAPAAGIDEDPVTGS
                     AHCTLGPFWGRRLGKSALTGRQISQRGGIVRCRLAEGRVFLSGRAVTVLRGELLA"
     gene            complement(2108..>4032)
                     /locus_tag="VNL92_07780"
     CDS             complement(2108..>4032)
                     /locus_tag="VNL92_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HWO94648.1"
                     /translation="VADSLDGLPVPYYVEPGREDDARRAFGRTPEMIRFFQERIGVPY
                     PYEKYAQVAVADFIFGGMENTSATTMTDTILYDERAAIDMDRDSLVAHELAHQWFGDL
                     LTCKEWAHAWLNEGFATYFDLLFLEHHKGQDEFAYGRFQNATVYFGEDSGHYRRPIVQ
                     RVYHEPIDIFDRHLYEKGSLVLHMLRYVLGDELWWKAIRHYTTKHAQGVVSTPDFQRA
                     IEEATGRNLDQFFDQWVYKGGHPEYKVSWSWDDETRVATLNVSQQQKTDDLTPVFAMP
                     FAVDFRSGEQSREFRVMVDQAEQTLHFPLDWRPDMVRFDPGNHVLKTLDFDKPVDELI
                     RQLDHDPDPMGRIHAALALGKKGGTKATEALAACVRSDAFWGVRAEAAKALGSMRTEA
                     ALTALAALSTVEHPKARRGVVRALGEFRHSERAAHALLPLLQSDPSYFVEGEAALSLG
                     KTRSVRAYDAITAAFAKESYNETIRVMACSGLAELRDERAIDFLLGWVPYGKPQQVRS
                     AAISALATLGGHVIERKKEIADALIGLLNDPWLRTRLAAADALGKLGDGAALGPLDEY
                     ASRELDGRGVRIAREAMAAIREGTSRDDELRKLREDLDRSMAENRDLRSRLDKIEAQL
                     NGRQDGRAAAIETMNA"
BASE COUNT          631 a         1338 c         1351 g          712 t
ORIGIN      
        1 acagaccatg ggcgccggcc cgcgaacggt cggcacgagc gacttcttct tcgccgcgtg
       61 cctcacgtat ctggaagagc acggcctcga tgcggagcag gtggaggcgt gggagggcgt
      121 gagttttcac gccctcgacc catggctccg catcgccaac gagctaccgg agggtcagga
      181 gatcgacctc ggatggctgg aggagctggc gcgcgtctgc aacgaggagg cggtctaccg
      241 ccgcttcgtc gagggtgtca gggcgcagga aggatagcag cgatgtggtg gcaagattgg
      301 ctctgggggt tctggaatgg cctcacggcc tgggtggtgc tcatcgtaca cgccttcggc
      361 gcctgggagg actttccgtt ctacaacgtc ggccggcgcg gcaactggta cgacttcggg
      421 tttgtcctgg gcgctggctc gccgttcttc ggggccctcg ggagcaggcg acgaggctaa
      481 ggcctgcgca atcaggcatc gccaggaatc gccgcatgga cttggcgggg ccgctgtagc
      541 tcgcagagct cagcgctgcg ctcgggcgtg ggtcgccagc accaggtcgt atcgagcctg
      601 gaggttgacc catatcgacg ccggaatccc aagcacctgc tcgagctcga gcgcggtctc
      661 cgcggtcacg cgcttcttcc cacgcacgat ctcgttgatg gccggtgccg gccgccccat
      721 ccgccgggcc aactcgcgct gagagatgcc ttcgctttcg agcgtttcgc gcagcagggc
      781 gcccggatgg gtggcctcat cggggataat gtcgtttgcg gatttcatcg tcatggcttt
      841 aatcgtcata atggttcgtt acctcatcca ctcggactgt ccgtccatcg ggcgatggtg
      901 tgacaacgag ccgccagcgc ccgacaagtg tcatcgcgta cgatccggcg cgattacccc
      961 gcaggggatg aaggcgcagc gggggcaccc ggaacagaag atcgaaggtg tcagccgcaa
     1021 tcatgagctc aatgcgctgc agatatcggc tggcgacctc gcgtccccag gcgcgttcgg
     1081 cctcgcggac gtgctcgtac cttcgggcca gctgccgcgt cgcgaaatcg acacgcacat
     1141 cgtgtacacc ctaagggata acacctctcg tgtcaagtaa ggccagacga gcatgagcga
     1201 tacgagggta tccgtcttca cggtggatgc gttcgccgat cgggcctttc gtggcaaccc
     1261 cgccgcggtg gttccgctcc ggcaggcggc gagccgcgcc tggatgcaaa acgtcgcggc
     1321 ggagatgaac ctttccgaga ccgcctttct cgaaccgatg gcggggtcgg ccgaggaacc
     1381 ccggttcggc ttgcgctggt tcacgccgaa gaacgaggtc gagctctgcg ggcacgccac
     1441 actggcgagt gcgcacgtcc tttggtccga aggactggcc tcgccttctg cgccggttgt
     1501 cttcgagacg ctctccggtc cgctgcgctg ccggcgcctg gaggacggct ggatcgagat
     1561 gagctttccg cccgagccac cactccccgt cgagcagccc cacgaagcgc tcctcgctgc
     1621 cttgcgcgtc gagccgcgat tcatcggccg cagtcgcttc gattacatga tcgaggtccc
     1681 gaccgaagac gaggtccgcg gcgtgacgcc ggacctgcgc ctcctccgga cggtgcccgt
     1741 gcggggcgtc tgcgtgacgg caccctcggc cgacccacgc tacgactttg tgtcgaggtt
     1801 cttcgccccg gcggcgggta tcgacgagga cccggtgaca ggctcggccc actgcacatt
     1861 gggaccgttc tgggggcgcc gcctcggcaa atcggcgctg accggccgcc agatctccca
     1921 gcgcggggga atcgtacgct gtcgtctggc ggaaggccga gtgttcctca gtggccgcgc
     1981 cgtcacggtg ctgcgcggag agctcctcgc ctagccctcg gacgacgtca tctcgagcag
     2041 attgctatcc ggcctcgatg cggcccggag cgggtcgggc gcccacctac ggagcgccgc
     2101 gcacgcctca cgcgttcatc gtctcgatcg ccgcggcgcg tccgtcttgc ctcccgttga
     2161 gctgcgcctc gatcttgtcg agccgcgatc gcaggtcgcg attctccgcc atcgaccgat
     2221 cgaggtcctc gcgcaacttg cgtagctcgt catcgcgact ggtgccctct cgaatggcgg
     2281 ccatggcctc gcgggcgata cggactccac gcccgtcgag ctctcgcgac gcatactcgt
     2341 cgagcggacc gagcgctgcg ccgtcgccca gcttccccag ggcgtcggcg gcggcgagac
     2401 gtgtccgcag ccacggatcg ttcagaaggc cgatgagcgc gtcggcgatc tccttcttcc
     2461 gctcgatgac gtggcctccg agcgtcgcga gggccgagat ggccgccgag cggacctgct
     2521 ggggcttgcc atagggcacc cagccaagca ggaagtcgat ggcgcgctca tcgcgcagct
     2581 ccgcgagacc agagcacgcc atgacgcgaa tcgtctcgtt gtacgactcc ttcgcgaagg
     2641 cggccgtgat ggcatcgtaa gcacgaacgg agcgtgtctt gccgagcgag agggcggcct
     2701 cgccctccac gaagtagctg ggatcgctct gcagcagcgg caggagggcg tgagcggcgc
     2761 gctccgagtg gcggaactcg ccgagggcgc gcacgacccc acgccgcgct ttcggatgct
     2821 cgaccgtgct cagggcggcc agggccgtca gcgccgcctc cgtgcgcatg gaaccgagcg
     2881 ccttcgctgc ttccgcgcgc acgccccaga acgcgtcgga gcggacgcac gcggcgagcg
     2941 cctcggtcgc tttcgtgccg cccttcttgc cgagcgcgag ggcggcgtga atgcgcccca
     3001 tcgggtccgg atcgtggtcg agctggcgta tcagctcatc caccggcttg tcgaaatcga
     3061 gcgtcttcaa cacgtggttg cccggatcga accgcaccat gtccgggcgc caatcgagcg
     3121 ggaagtgcaa cgtctgctcc gcctggtcga ccatcacgcg gaactcgcgg ctctgctcgc
     3181 ccgatcggaa gtcgaccgcg aagggcatgg cgaagacggg cgtgaggtcg tccgtctttt
     3241 gctgctgcga gacattcagc gtggcgacgc gcgtctcgtc gtcccagctc cacgagacct
     3301 tgtactccgg gtggcctccc ttgtagaccc actggtcgaa gaactggtcc aggttgcgcc
     3361 ccgtcgcctc ctcgatggcg cgctggaaat cgggagtcga tacaactccc tgcgcgtgct
     3421 tcgtcgtata gtgccggatc gccttccacc acagctcgtc gccgagcacg tagcgaagca
     3481 tgtgcagcac aagcgagccc ttctcgtaca ggtggcgatc gaagatgtcg atgggctcgt
     3541 gatagacgcg ctgcacgatg ggccgccggt agtgccccga gtcctcccca aagtagaccg
     3601 tggcgttctg gaatcgcccg tatgcgaact catcctgtcc cttgtgatgc tcgaggaaca
     3661 gcagatcgaa gtacgtcgcg aagccctcgt tcagccacgc atgggcccac tccttgcacg
     3721 tcagtaggtc gccgaaccac tgatgggcga gctcgtgcgc gacgagcgag tcgcggtcca
     3781 tgtcgatcgc ggcgcgctcg tcgtagagga tcgtgtcggt catcgtcgtg gcgctggtgt
     3841 tctccatgcc gccgaagatg aagtcggcga ccgccacctg cgcgtacttc tcgtacgggt
     3901 agggaacgcc gatacgctcc tggaagaagc ggatcatctc gggcgtgcgg ccgaaggcgc
     3961 ggcgggcgtc gtcctcccgg cccggctcga cgtagtacgg cacgggcagg ccatcgagcg
     4021 agtcggcgac tt
//
LOCUS       DATMBB010000364         2791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_487521, whole genome shotgun sequence.
ACCESSION   DATMBB010000364 DATMBB010000000
VERSION     DATMBB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487521"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VNL92_07785"
     CDS             <1..1017
                     /locus_tag="VNL92_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO94649.1"
                     /translation="ADAGMLAVAWPKEYGGAGLTPLEQVILAEEFAKADVPTQGGNDG
                     FGIGLVGPTIIVWGTEEQKRHFLPRIISGEDRWCQGYSEPDAGSDLANLGTRAVLDGD
                     EWVINGSKMFITNAGLENCAFTTVVCLTDPETKEFNTILVPSGTPGMHILPPLKKMGL
                     RSSDTRQIIFDDCRVPRMNLMGERGRGRERTVVGFFTARTTLSSTALGVAEECFRLAL
                     DYAKQRRAFKRRLTDFQYIQGYLVDMSMNIELSRLLRDRTARMIMEHDEEVPRFAAMS
                     KYFCCETAKQATDHAVQIFGGMGFMDDAAVSRYYRDIRAATIADGTTEIQKFIIARSL
                     GMFS"
     gene            1121..1711
                     /gene="infC"
                     /locus_tag="VNL92_07790"
     CDS             1121..1711
                     /gene="infC"
                     /locus_tag="VNL92_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWO94650.1"
                     /translation="MSRELRTNERIRIPQVRVIDESGAQLGVMPTDRALTLARERGLD
                     LVEVAPTADPPVCRILDFGKFKYEQSKREREARKNQKQVVLREVRMKPKIDEHDIAFK
                     TRTAAKLLREGDKVKVSVMFRGREVTHPQIGRDLLARVYAELKDFSVIEKQPSLEGRF
                     MTMILAPGAPPQRPKERPSSTSEPEPEPERTGATSA"
     gene            1704..1904
                     /gene="rpmI"
                     /locus_tag="VNL92_07795"
     CDS             1704..1904
                     /gene="rpmI"
                     /locus_tag="VNL92_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWO94651.1"
                     /translation="MPKLKTHKGAKKRFGVTGSGKFTRRKGGISHNRTRKSARALRDY
                     DEMLVVHPTYQKRLQRLMPYAK"
     gene            1914..2270
                     /gene="rplT"
                     /locus_tag="VNL92_07800"
     CDS             1914..2270
                     /gene="rplT"
                     /locus_tag="VNL92_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWO94652.1"
                     /translation="MARVKRGVAAHARHKKVLVQTRGHKGQRHRLFRRANESLLHALQ
                     YQYRDRRDRKGQFRRLWIARINAAARLHGISYSRMIDGLNKAGVEIDRKVLADLAVRD
                     DHAFGELAARAKAALA"
     gene            complement(2292..>2791)
                     /locus_tag="VNL92_07805"
     CDS             complement(2292..>2791)
                     /locus_tag="VNL92_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWO94653.1"
                     /translation="LRFAADELPGLLPGRSAVIDGGMERLGVLGELDPSIAAAFDFRG
                     GGYLFEIDVGAVARALERHPRRYQPVSRYPAVEEDLSFLVDAACPAGDLAAEIEAAPL
                     VERAAVFDVYTGEGLPAGKKSIAFSVTYRAPDRTLTNDDIGRTRRRLVERLAREFGAE
                     LRGGP"
BASE COUNT          562 a          886 c          888 g          455 t
ORIGIN      
        1 gccgacgcgg gcatgctggc ggtggcgtgg cccaaggagt acggcggcgc gggcctgaca
       61 ccgctcgagc aggtgatcct cgccgaggag ttcgccaagg ccgacgtccc cacgcaaggc
      121 ggcaacgacg gcttcggcat cggcttggtc ggccccacga tcatcgtgtg gggcaccgag
      181 gagcagaagc gccacttcct gccccgcatc atcagcggcg aggaccgctg gtgccagggc
      241 tactccgagc cggacgcggg gtccgacctc gccaacctcg gcacccgtgc ggtgctcgat
      301 ggcgacgagt gggtgatcaa cggctccaag atgttcatca ccaacgcagg cttggagaac
      361 tgcgcgttca ccaccgtcgt ttgcctgacc gacccggaga cgaaggaatt caacacgata
      421 ctggttccct cgggcacgcc aggaatgcac atcctgccgc cgctcaagaa gatgggtctg
      481 cgttcgtccg atacgcgcca gatcatcttc gacgactgcc gcgtgcctcg catgaatctg
      541 atgggcgagc gcggtcgagg gcgggaacgc acggtggtcg gattcttcac cgcgcgcacg
      601 acactatcgt cgaccgcact cggtgtcgcc gaggagtgct tccgtttggc gctcgactac
      661 gcgaagcaac gccgcgcttt caagcggcgg ctcaccgatt ttcagtacat ccagggctat
      721 ctcgtcgaca tgtcgatgaa catcgagctc agccgtctcc ttcgtgatag gactgcccgg
      781 atgattatgg aacacgacga ggaggtgcct cggttcgccg cgatgtcgaa gtacttctgt
      841 tgcgagacgg cgaagcaggc gaccgaccac gccgtgcaga tcttcggagg catgggattc
      901 atggatgacg ccgccgtttc tcggtactac cgagacatcc gcgccgcaac catcgcagac
      961 ggcacgacgg agatccagaa gttcatcatc gcgcgctccc tgggaatgtt ttcgtagcgt
     1021 cgatccccaa ttgtgggcct caaaccgtta tggtacgctt gccgccactg atatactgaa
     1081 cgtgcaaacc tactgaaggc tcactggagg aacgcagctc atatcacgag aacttcgtac
     1141 gaacgaacgt attaggatcc cgcaggtccg ggtcatcgac gagagcggtg ctcagctcgg
     1201 ggtcatgccg acggatcgag cgctcaccct ggcacgggaa cgcgggctcg acctggtaga
     1261 agtagcgcca accgccgatc cgcccgtctg ccgcattctc gatttcggga agttcaagta
     1321 cgagcagtcg aagcgcgagc gcgaggcgcg gaaaaaccag aagcaagtcg tcttgcgcga
     1381 agtgcgcatg aagccgaaaa tcgatgagca cgacatcgcc ttcaaaactc gtacggcagc
     1441 caagctgctg cgcgagggcg acaaggtcaa ggtatcggtc atgttccgcg ggcgcgaggt
     1501 gacccacccg cagattgggc gggacctcct cgcccgcgtg tatgccgaac tcaaggactt
     1561 ctccgtcatc gagaagcagc cctcgctgga agggcggttc atgacgatga tacttgctcc
     1621 aggagcgccg ccgcagcgac ctaaagagcg tccatcttcg acgagcgagc cggagccaga
     1681 accggaacgg acaggagcga cgagtgccta agctgaagac acataagggc gcgaagaagc
     1741 gttttggggt gactggctct gggaagttca cccgccggaa gggcggtatc agccacaacc
     1801 ggacccgcaa atcggcacgc gccctgcgcg actacgacga gatgctcgtc gtccacccca
     1861 cataccagaa gcgactgcag cggctcatgc cgtatgcgaa gtaggagata gggatggccc
     1921 gcgtgaagcg aggcgtggcc gcgcatgccc gccacaagaa ggtgttagtc cagacacggg
     1981 ggcacaaggg gcagcgtcat cgcctgtttc gacgtgcgaa cgagtccctc ctgcacgccc
     2041 tgcagtacca gtaccgcgat cggcgcgatc ggaagggaca gttccgacgg ctgtggattg
     2101 cgcgcatcaa cgcggccgcg cggttgcacg gcatctccta cagccggatg atcgacggac
     2161 tgaacaaggc cggcgtggaa atcgaccgca aggtactggc cgaccttgcc gtgcgcgacg
     2221 accatgcgtt tggcgagctg gcggcgcgtg caaaggcggc actggcttag gcgtagcgcc
     2281 gcttcggcga ttcaggggcc gccgcgcaac tccgctccga attcccgagc gagccgctcc
     2341 accagtcgac gccgagtccg cccaatatcg tcattcgtga gcgtgcggtc gggcgcgcga
     2401 taggtgacgc tgaaggcgat cgatttcttt cccgccggca gtccctcccc cgtgtagaca
     2461 tcgaagaccg ccgcccgctc gacgagcggc gcagcctcta tttccgctgc gaggtcgccg
     2521 gctggacagg ccgcatcgac gaggaacgac aggtcctcct ccacggccgg gtacctcgat
     2581 acgggctggt aacgacgagg gtgtcgctcc agcgctcgag ccacggctcc gacgtcgatc
     2641 tcgaacagat atcccccgcc ccgaaagtcg aacgccgcag cgatgctcgg gtcgagttcg
     2701 ccgagaacgc cgagtctttc cattccgcca tcgataacgg ccgagcgccc tgggagcagg
     2761 cccggcagct catcggccgc aaagcgtagg g
//
LOCUS       DATMBB010000365         8618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_98667, whole genome shotgun sequence.
ACCESSION   DATMBB010000365 DATMBB010000000
VERSION     DATMBB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 8618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8618
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98667"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VNL92_07810"
     CDS             complement(<1..238)
                     /locus_tag="VNL92_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWO94654.1"
                     /translation="MSETSRTQSQFGERISALPSGPGVYIFRNDRDQVIYVGKAANLR
                     NRVRSYFGSPRSMEPKTSALVQQIADFEYIVTPSV"
     gene            347..724
                     /locus_tag="VNL92_07815"
     CDS             347..724
                     /locus_tag="VNL92_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWO94655.1"
                     /translation="MPKRIMIINDDTATVTLMKRLLEEEGYSVIFQHEGSLAYPAIQH
                     ERPDLVLLDLRLTRPDEGWRVLEAMKLDRDTTHIPVVVCSADRQQLEASSLRLRELNC
                     DILEKPFSLDHLLAKVRERLAST"
     gene            818..1801
                     /locus_tag="VNL92_07820"
     CDS             818..1801
                     /locus_tag="VNL92_07820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWO94656.1"
                     /translation="MAELGYTLSTEEFAAPALVSNARRAEEAGFTFALISDHFHPWVE
                     RQGQSPFAWTVLGGVAEATERLRIGTGVTCPTIRYHPAIVAQAAATVADLMPGRFFLG
                     LGTGENLNEHVVGQGWPEPAVRREMLDEAVHIIRTLWQGDTVSYRGSYFTVERARIYS
                     LPEKLPDIYIAASGPRTAKLAGAVADGFIGVAPDASLIKAFREAGGAGKSCIGQVNVC
                     WAETEEQARRTAIEWWPNAAVSGETSLILQSPSDFDALAKLTRDEEKVKGFALGPDPE
                     VHLATLRAYVEAGYTHVYVHQVGPDQEGFFRFYERNILPEFQRQLVRAEPA"
     gene            1951..2826
                     /locus_tag="VNL92_07825"
     CDS             1951..2826
                     /locus_tag="VNL92_07825"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWO94657.1"
                     /translation="MSVAEPEITSRGYAHPEVLVTTQWVADHLQSPDVRIVESNEDPL
                     LYSSGHVPGAVNIDWAADLNDPLRRDYLNREGFERLMSRSGITPDTTVVLYGDKNNWW
                     ATYAFWVFQLFGHKNVRIMDGGRLKWEKEGRPLTREVPTYPPTQYRASERDDTRIRAF
                     RDEVLAHVQAGKPLIDVRSPQEYSGERLHMPDYPNEGALRGGHIPGAKNVPWARAINP
                     DDGTFKPAEELRQIYQGEVGLKPEDEVIAYCRIGERSSHTWFALTYLLGFDKVRNYDG
                     SWTEWGNLVNVPIER"
     gene            2836..3306
                     /locus_tag="VNL92_07830"
     CDS             2836..3306
                     /locus_tag="VNL92_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HWO94658.1"
                     /translation="MPTREGVPAALNELLADFDFLDRTERTQMLLELADRFREVPPEV
                     ASRPYPEEHRVQKCESQAYVWAQDLPDGRLKFHFAVENPQGISAKAMAVILDEALSDQ
                     PLEEVARVPSDIIFDIFGREVSMGKGEGLSGIVSLVTAEARRRLAERDEGNGAS"
     gene            3451..3918
                     /locus_tag="VNL92_07835"
     CDS             3451..3918
                     /locus_tag="VNL92_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWO94659.1"
                     /translation="MIEQVFRTDPIRSLGLPEPVSIGRNAKVGEALAAVQRHGMGYVL
                     VVDEAGRPVGILSETEVLMRIVARDVRFDESVEKFVSGEMHTLTARDTVARGIQIMAE
                     GEERNLPIVDEEGRAVAVLRTLDIIHFLAEAIPEQVLNLPPRPHQLMPKPEGG"
     gene            3918..5780
                     /locus_tag="VNL92_07840"
     CDS             3918..5780
                     /locus_tag="VNL92_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWO94660.1"
                     /translation="MTTLTKNAQQPSRHEVLELDRVTIRFAGDSGDGMQLAGTQFTKT
                     SAVVGNDISTLPDFPAEIRAPQGSLPGVSGFQVSFSSHEIYTPGDEPDVLVAMNPAAL
                     KTNLSDLPRGGILVVNEDEFTETNLKKAGYEANPLEDGSLQAYRVYEVPISTLNARAL
                     EESGLTPVQIDRAKNMFALGLMFWMYSRPLDVTIRWIEEKFGKNPQVAQANITTLKAG
                     YHFGETTEMFATHYHVPKAPAHPGTYRNITGNEATALGLVTASKLAGRPLLYASYPIT
                     PASDVLHELAAMKRFGVRTLQAEDEISAVGAAIGAAFGGSLGTTGTSGPGFALKSEAI
                     GLAVMVELPLVVLDIQRAGPSTGMPTKMEQGDLLQAMFGRNGESPVAIIAPATVSDCF
                     SMVIEAWRIAIKYRVPVIFLSDAYLGMGSEPWLIPSFSELAPIDANFASDPETFQPYL
                     RDEQTMARPWAIPGTPGLEHRIGGLEKAHISGTVNYQPDNHDLMVHLRAEKVQRIVQD
                     VPDVEVDGDESGELLVLGWGSTYGAITSAVQRARAEGKSVSAAHLRYLNPLPANLGEV
                     LGRYKKVLIPENNLGQLQLLVRGRYLVDAVGLNKASGRPFTISEVEQKIDELLS"
     gene            5801..6838
                     /locus_tag="VNL92_07845"
     CDS             5801..6838
                     /locus_tag="VNL92_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWO94661.1"
                     /translation="MVTTNGNPAITLTRKDFVSDEEVRWCPGCGDYSILAQTQRVMPE
                     LGIPREDIVFISGIGCSSRLPYYMNTYGFHSIHGRAPTIATGLKVTRPELSVWVITGD
                     GDALSIGGNHILHVMRRNVDLQIVLHNNEIYGLTKGQYSPTSRLGQRTKSSPMGAIDM
                     PLNPLSVAIGAEGTFVARSIDTDTTHLQSTHLRAYQHHGASFVEVYQNCNIFNDGAFA
                     DFAAKEVRADRTLQLEHGKPMVFGKERNKGVRLNGLQPEIIDLGGPDGYNEGDCLIHD
                     EASEDPTIHFMLSRMHYPSHPVPVGVIRAVKRATYDELMAKQIDDAIAREGEGDLQDL
                     FSQGDVWTVEA"
     gene            6845..7351
                     /locus_tag="VNL92_07850"
     CDS             6845..7351
                     /locus_tag="VNL92_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWO94662.1"
                     /translation="MICPECRHDNIEGVDYCENCGQDLRGLDQPDSGLESGPGFLHER
                     LSDLHHEPMVAVRPSDPVGLAVRVMQNEGRTCILVMERGELLGIITPFDILQKVAGPR
                     EDLNAVTCAQVMTADPVCVRDEDDIAVALNKMSIGAFRHLPLLQDGRPIATIGIDAVF
                     QRIAPFLA"
     gene            complement(7396..8418)
                     /locus_tag="VNL92_07855"
     CDS             complement(7396..8418)
                     /locus_tag="VNL92_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWO94663.1"
                     /translation="MPSSSHPHIDDLIIIGAGPTGLFAAFYAGLRHMKTKLVEALPEP
                     GGQLAVLYPEKFIYDVPGHPKVLAKDLVALLMEQIALFNPEMVFDTRIDTCTRASVDG
                     EEVWRLGTATEAHYCRTVLITAGIGAFEVNRLDRPGVEEYEGRGVYYFVKDKRPFRNK
                     RVLIIGGGDTAVDWALNLNHWASHITLIHRRDQFRAHEASLAELRLSEISVMTHWELK
                     RVYGDSGSITGATIYENRIGEERDLDVDIVLINIGFKAALGPIASWGLEMVDARHIRT
                     NGHMETNLPGVYAAGDIAAVEGSTPLNLIVTGFGQAAIAANAARAYIDPKARMFPGHS
                     SEMRLS"
BASE COUNT         1676 a         2677 c         2747 g         1518 t
ORIGIN      
        1 ggacgctggg cgtgacgatg tattcgaaat cggcgatctg ctgaacgagg gcgctcgtct
       61 tcggttccat gcttcgtggc gagccgaagt acgaccggac tcgattccgc aggttggcag
      121 ccttcccaac gtagatcacc tgatcgcgat cgttccgaaa gatgtacacg cccggacctg
      181 acggtagggc ggagatgcgc tcgccgaatt gcgattgtgt ccgactcgtc tcgctcatgt
      241 tcgtaatcat tgtacttctg cggctccggc tccggcgaac gccggcccaa ccgggagcgc
      301 gattgttgcg agtgttacac tatgcggaca agcagaaaga gggttcatgc cgaaacgaat
      361 catgatcatc aacgacgata cggccaccgt cacgctcatg aagcggttgt tggaggagga
      421 aggctatagc gtaatcttcc aacacgaggg atccctcgct tatccggcga ttcaacacga
      481 gcgtcccgac ctcgtccttc tcgacctgcg cctgacgaga cccgacgagg gctggcgcgt
      541 actggaggcg atgaaactcg accgcgacac cacccacatc cccgtcgtcg tgtgctcggc
      601 tgatcgacag cagctcgagg caagctcatt gcggctacga gagctcaatt gcgacattct
      661 cgagaagccc ttcagtctgg atcatttgct ggcgaaggtg cgggaaaggc tcgcaagcac
      721 ctgatgccgt caaagcgcaa ctccgctccg gttgctgtta tgtggctgca atcacaccag
      781 ggcgatcctc tctatgacat tccacgagag gtgcccgatg gcggagctcg gctacacact
      841 atcgacggaa gaattcgcgg cgcctgcact ggtcagtaac gcccgccgtg cggaggaggc
      901 gggcttcacc ttcgccctca tctccgatca cttccacccc tgggtggagc gccaaggtca
      961 aagtccgttc gcttggaccg tgctgggtgg cgtcgccgag gcgaccgaga ggctgcgcat
     1021 aggcaccggg gtcacctgcc caacgatccg gtatcaccca gccatcgtcg cccaggctgc
     1081 tgcgactgtc gctgatctga tgcccggccg cttcttcctc ggactcggca cgggtgagaa
     1141 cctgaacgaa cacgttgttg gccagggatg gccggagccc gccgtgcggc gagagatgct
     1201 cgacgaggcg gtgcacatca tccgaacgct ctggcagggc gataccgtga gctaccgagg
     1261 gtcctacttc accgtcgagc gtgcgcgcat ctactccctg cccgaaaagc tgccggacat
     1321 ctatatcgcg gccagtggac cacggacggc caagcttgcg ggagctgtgg ccgatggctt
     1381 catcggcgtt gcccccgacg cttcgctcat caaggcattc cgcgaggccg gcggcgctgg
     1441 aaagtcgtgc ataggccagg tcaatgtctg ctgggccgag accgaggagc aggctcgccg
     1501 tacggccatc gagtggtggc caaacgcggc cgtgagcgga gagacttcgc tcatccttca
     1561 atcgcccagc gacttcgacg cgctggccaa gctcacgcgc gacgaagaga aagtgaaggg
     1621 gttcgcgttg gggcccgacc ctgaagtgca ccttgccacg ctgcgtgcct acgtggaggc
     1681 cggctatacc catgtctacg tccatcaggt agggcccgac caagaaggct tcttccgctt
     1741 ctacgagcgc aacatccttc cggagtttca gcggcaactc gtgagggccg agccagcgta
     1801 gcggctgcaa gacgcgaggc cgctcgatag cgtccaccgc tcctcgccgg gcttcgatcg
     1861 cgatgcctcg ccgcgaacgc gaggagggct tcctgcgtgc tagactaagg ggagtaaatg
     1921 gagcgacaaa gtacagaagg aggcgccgtc atgtccgtcg cagagcctga gatcacgagc
     1981 cgcggctacg ctcatccaga ggtgctcgtc accacgcaat gggtggccga ccatctgcag
     2041 agcccggacg ttcgcatcgt cgagtcgaac gaggatccgc tgctgtactc atcgggccac
     2101 gttcccggtg cggtgaacat cgactgggca gccgatctga acgatcctct ccggcgcgac
     2161 tacctgaacc gggaggggtt cgagcggctg atgtcgcgca gcggcattac gcccgacacg
     2221 acagtcgtgc tgtacggcga caagaacaac tggtgggcaa cgtacgcgtt ctgggtcttc
     2281 cagctgttcg ggcacaagaa cgttcgcatc atggacggcg ggcgactcaa gtgggagaag
     2341 gaggggcgcc cgctgacgcg tgaggtgccg acgtatccgc ctacgcagta ccgcgcctcc
     2401 gagcgcgatg acactcgaat tcgcgccttc cgcgatgagg tgctcgcgca cgtgcaggca
     2461 ggcaagccgc tcatcgatgt ccggagcccg caggaatact cgggcgaacg gctgcacatg
     2521 cccgattacc cgaacgaagg agcgctacgc gggggccaca tcccgggggc gaagaacgtc
     2581 ccgtgggctc gggcgatcaa tccggacgac ggcacgttta agcccgccga ggagttgcgc
     2641 cagatctacc agggcgaagt tggcctgaag cccgaggacg aagtcatcgc ctactgccgc
     2701 atcggggagc gaagcagcca cacgtggttc gccctgacgt acctgctcgg tttcgacaag
     2761 gttcgcaact acgacggctc gtggaccgag tggggcaatc tggtcaacgt tccgatcgag
     2821 cgatagtatg gaggtatgcc gacgagagaa ggtgttcctg ccgcactgaa cgagttgctg
     2881 gcggacttcg actttctcga ccgaacggaa cgcacccaga tgctcctgga gctcgccgat
     2941 cggttccgcg aggtgccccc ggaggtcgca tcccggccct atccggagga gcatcgcgtt
     3001 cagaaatgcg agtcgcaagc gtacgtgtgg gcgcaagacc tgccggatgg caggctgaag
     3061 ttccactttg ccgtggagaa cccgcagggc atttcggcca aggcgatggc ggtcatcctc
     3121 gacgaagcgc tgtcggacca gcccctcgaa gaggtggctc gggtgccgag cgacatcatc
     3181 ttcgacatct tcggacgcga ggtttcgatg gggaagggcg agggactgag cggcatcgtg
     3241 tcgctggtca cggcggaagc ccgccggcgc ctcgcggaac gcgatgaagg caacggtgcg
     3301 tcgtagcgta ggtggttcca gtaggggcga catgctgctc gagttatcga agcagtcgat
     3361 ttggccgatg gcacagactg cgatacaatg aatgcagcgg ctaccgacgt cggcggccgt
     3421 ccgcgctgag gggaaggaca ccgaggcgac gtgatcgagc aggtcttccg caccgacccc
     3481 attcgctctc tcggactccc tgaacccgta tccatcggac gcaacgccaa ggttggcgaa
     3541 gcgctcgccg cggtgcagcg ccacggcatg ggctacgtgc tcgtcgtcga cgaggcggga
     3601 aggccggtcg gaatcctcag cgagaccgaa gtgctcatgc gcatcgtggc tcgggacgtg
     3661 cgcttcgacg agagcgtcga gaaattcgtg tcgggagaaa tgcacacact gacggcccgg
     3721 gacaccgtcg ccaggggcat ccagattatg gccgagggcg aagaacggaa cctcccgatt
     3781 gtggatgagg aggggcgagc ggtcgcagtg ctccgcacgc tcgacatcat ccacttcctc
     3841 gccgaggcga ttccggagca ggtgctcaac ctcccgcccc ggccgcacca actgatgccg
     3901 aaaccggagg gtggatagtg actacgctaa cgaagaatgc acaacaaccg tctcggcacg
     3961 aggtgctcga gcttgatcgc gtcaccatcc gctttgccgg tgattccggc gacggcatgc
     4021 agcttgccgg aacacaattc accaagacct cggccgtcgt cggcaacgac atcagcacgt
     4081 tgccggactt cccggcagag attcgggccc cgcagggctc gctgccgggc gtaagcggtt
     4141 tccaggtcag cttctcaagt catgagatct atacgccggg agacgagccc gatgtcctcg
     4201 tggcgatgaa tccggccgct ctcaagacga acctttcgga tttgccacgc ggcggaatcc
     4261 tcgtcgtcaa cgaggacgaa ttcacggaga cgaacctgaa gaaggccgga tacgaggcca
     4321 accccctcga ggatggcagc ctccaagcgt accgcgtgta tgaagtgccc atctccacgt
     4381 tgaatgcccg cgcgctcgag gagagcggcc taacgccggt ccagatcgac cgggcgaaga
     4441 acatgttcgc gctcggcctc atgttctgga tgtacagccg cccgctggat gtgacgatcc
     4501 gctggatcga ggagaagttc ggcaagaacc cgcaagtcgc tcaagcgaac atcacgacac
     4561 tcaaggccgg ctaccacttc ggcgaaacga cggagatgtt cgccacgcac tatcacgtgc
     4621 cgaaggcgcc cgcccacccc ggcacctatc gcaacatcac cggcaacgaa gcgacggccc
     4681 tgggcctcgt gacggcgtcg aagcttgctg gtcggccgtt gctctacgcg agctacccca
     4741 ttacgccggc gagcgacgtg ctccacgagc tggcggcgat gaagcgcttc ggtgttcgca
     4801 cgttgcaggc cgaggatgag atatcggcag tgggcgcggc catcggcgct gccttcggtg
     4861 gatcgctcgg cacaacgggc acgagcggcc ctgggttcgc cctgaaaagc gaggctatcg
     4921 gcctggcggt gatggtcgag ctgccgctgg tggtcctcga tattcagcgt gcgggcccca
     4981 gcacgggcat gcccaccaag atggaacaag gcgacttgct ccaggcgatg ttcggtcgta
     5041 acggcgagtc cccggtggcc atcatcgcgc cagcgaccgt atcggactgc ttctcgatgg
     5101 ttatcgaggc ttggaggatc gccatcaagt accgggtgcc ggtcatcttc ctctccgatg
     5161 cctaccttgg tatgggctcc gagccctggc tcatcccgag cttcagtgag ctcgcgccaa
     5221 tcgacgccaa ctttgcgagc gaccccgaga cgttccagcc gtacctgcgt gacgagcaga
     5281 cgatggcgcg gccgtgggcg attccgggta cgcctgggtt ggagcaccgc atcggcgggc
     5341 tcgagaaggc gcacatctcc ggcacggtca actaccagcc cgacaatcat gacctgatgg
     5401 tgcacttgcg ggcggagaag gtgcagcgca ttgtccagga cgtgccggac gtggaggtgg
     5461 atggtgacga gtcgggcgag ctgctcgtgc tcggctgggg cagcacgtat ggcgccatca
     5521 cgagcgccgt gcaacgagca cgggcagaag gtaagagcgt ctcggcagcc caccttcgct
     5581 acttgaaccc acttccggcg aatcttggcg aggtgctggg ccgctacaag aaggtgctca
     5641 tcccggagaa caacctcgga cagctacagc tcctcgtacg cggccgctac ctcgtcgacg
     5701 ccgtagggct gaacaaggct tccggccggc ctttcaccat ctcggaagtc gaacagaaga
     5761 ttgacgaact cttaagctga atgaagcagg cgatagaatc atggtaacga caaacgggaa
     5821 ccccgcaatc accctcacta ggaaggactt cgtctccgac gaggaagtcc gatggtgccc
     5881 cggctgcggc gactactcga tcctcgcgca gacgcagcga gtcatgccgg agctcggcat
     5941 cccacgggag gacatcgtct tcatctccgg catcggatgc tccagccggc tgccgtacta
     6001 catgaacacg tacggcttcc acagcatcca cggtcgcgct ccgacgatcg cgacgggcct
     6061 gaaggtgacg cgtcctgagt tgagcgtctg ggtcatcact ggcgacggcg atgcgttgag
     6121 catcggcggc aaccacatcc tgcacgtgat gcggcgcaac gtcgatctgc aaatcgtcct
     6181 gcacaacaac gagatctacg gcctcacgaa ggggcagtac tcgccgacgt cgcggctcgg
     6241 ccagcgcacg aagtcctcgc ccatgggcgc catcgacatg ccgttgaacc cgttgtcggt
     6301 tgccattggt gccgagggaa cgttcgtcgc tcgttcgatt gacacggaca cgacacacct
     6361 acagagcacg caccttcgag cctatcagca ccacggggca tccttcgtgg aggtgtacca
     6421 gaactgcaac atcttcaacg acggcgcctt cgccgacttc gctgcgaagg aagtccgcgc
     6481 cgaccgcacg ctccaactgg agcatggcaa gccgatggtc ttcggtaagg agcgaaacaa
     6541 gggcgtccgt ctgaatggac tgcagcccga gatcatcgac ctcggtggtc cggatggata
     6601 taacgagggc gactgcctga tccacgatga ggcgagcgag gacccgacga ttcacttcat
     6661 gctttcgcgg atgcactacc cgagccatcc cgtgccggtg ggcgtgatcc gagccgtgaa
     6721 gcgcgccacc tacgacgagc tcatggcgaa gcagatcgac gacgccattg cacgagaagg
     6781 cgagggtgac ctccaggatc tcttcagcca gggcgatgtc tggaccgtcg aggcctgagg
     6841 cacgatgatc tgtcctgagt gccgccacga taacatcgaa ggcgtcgact actgcgagaa
     6901 ttgcgggcag gacctgcgcg gcctggacca accggattcg gggctcgaga gcgggccggg
     6961 tttcctccac gagcgcttgt ccgatctgca ccacgaaccg atggttgccg tacggccgtc
     7021 cgacccggtc ggcttggccg tccgggtcat gcagaacgaa ggccggactt gcatcctggt
     7081 gatggagcgg ggcgagctgc ttggcattat caccccgttc gacatcctgc agaaggtggc
     7141 ggggccgcgt gaggacctta acgccgtgac gtgcgcccag gttatgaccg ctgatcccgt
     7201 ctgtgtccgc gatgaagacg acatcgcggt cgcgctcaac aagatgtcga tcggggcttt
     7261 ccgccacctg cctctgcttc aggacgggag gccgatcgcc accatcggca tcgacgccgt
     7321 gtttcagagg atcgcgccct tcctcgcgta gcttcgagtg tagttgcgag agggggcgtc
     7381 gggcggcggc cgccttcagc taaggcgcat ctcactcgag tgtcccggaa acatgcgtgc
     7441 cttcggatcg atatacgcgc gcgccgcgtt ggccgcgatg gcggcttggc cgaacccggt
     7501 aacaatcaga ttgaggggag tggagccttc gacggcggcg atgtcgcccg cggcgtagac
     7561 cccgggaaga ttcgtctcca tgtggccgtt cgtgcggatg tggcgggcat cgaccatctc
     7621 gagtccccaa gaggctatag gccccagtgc tgccttgaac ccaatgttga tcaacacgat
     7681 atcgacgtcg aggtcgcgct cctcgccaat ccggttctcg tagatcgtcg cgcccgtgat
     7741 ggagccgctg tcgccgtaga ctcgcttcag ctcccagtgc gtcatcaccg agatttcgct
     7801 caggcgcagc tccgcgaggc tcgcctcgtg cgctcggaac tgatcgcggc gatggataag
     7861 ggtgatgtgg cttgcccaat gattcaggtt cagggcccaa tcgaccgccg tgtcgccgcc
     7921 gccaatgatc agcacgcgct tgttgcggaa tggcctcttg tccttgacga agtagtacac
     7981 ccctcgccct tcatactctt ctacccccgg ccggtcgagg cggttcacct cgaatgctcc
     8041 gattccggcc gtgatcagga cggtgcggca gtagtgtgcc tcagttgccg tgccgagacg
     8101 ccaaacctcc tcgccgtcga cgctcgcgcg cgtgcacgtg tcgatccgcg tatcgaagac
     8161 catctcgggg ttgaacaacg cgatctgctc catgagcaag gcaacgaggt ccttggccaa
     8221 gaccttcggg tgtccgggga catcgtagat gaacttttcg ggatacagga ctgccaactg
     8281 gccgccaggt tcgggcaagg cctcgacgag cttcgtcttc atgtgccgga ggccggcata
     8341 gaaggcggcg aacaggcccg tgggtccggc gccgatgatg atcaggtcat cgatgtgcgg
     8401 gtgggacgac gacggcatgt gaggctcacg ctccagccgg tgtaggtgct gcacgcctga
     8461 ggcggccctc tcacactacc acagccctgc gcctcgttcg acgtgtgacg cctgttacaa
     8521 tcaagcgcgg aaaaacttct gggtggggag gagcatcgtg atcaagctca ccgaggagat
     8581 gcgcgagcac atcaacaatg cgctgatgaa tcgtgcac
//
LOCUS       DATMBB010000366         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_535411, whole genome shotgun sequence.
ACCESSION   DATMBB010000366 DATMBB010000000
VERSION     DATMBB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535411"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VNL92_07860"
     CDS             complement(<1..755)
                     /locus_tag="VNL92_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWO94664.1"
                     /translation="MNRLLLWLATAVAAVALAGGAAAILVVSGGDDDDDAGGGTSPTA
                     EEGPDLGDREGNELRLLGADPATLDPHIATDADSARYIVEVFSGLVTIDENLQVAPDI
                     AESWDVSADGTVYTFHLRRNVVFHSGKPVTAQTVKDSIERAASPETQSPVADVYLGDI
                     VGVRDKLRGRADEISGIRVIDDYTLEITIDAPKPYFIAKLTYPTAFVVDVEQIESNPR
                     NWTRRPNGTGPFVLSEWELGESIVLDANDRFYGG"
     gene            complement(867..>2542)
                     /gene="aspS"
                     /locus_tag="VNL92_07865"
     CDS             complement(867..>2542)
                     /gene="aspS"
                     /locus_tag="VNL92_07865"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWO94665.1"
                     /translation="DSTGLIQVVINPQEAPAAHAIGEDVRVEYVLRVRGVVRPRKPGT
                     ENASLPTGEIEVHAADIEILNPAKNPPFYVNEDQEVDELLRLKYRYIDLRRPVMHDRL
                     ALRHRAIRYIRDFLDERGFMEVETPVLIKATPEGARDFLVPSRMHPGEFYALPQSPQQ
                     LKQLLMVAGVERYYQIARCYRDEDLRADRQPEFTQLDLEMSFVEQDDVLSLMEEMFAG
                     LVRQVAPQFSVPSTFPRISYADAMERYGSDKPDVRYGMELHDLSDLFAGSEFSVFRSA
                     MESNGRVRGFAARGAADTPRRQLDELTSIAQMAGAKGLVWLAIGGDGDLENLTSEDIR
                     SPAARFLSADEVRAAARRCEAGRGDLLLMVADEYRRTSAVLDVLRREMAERLRLADPH
                     HLAFAFITDFPMFEWNDDEGRWDAVHHPFTAPHPDDLPFLESNPGRARALAYDLVCNG
                     WELGSGSIRIHERELQRSIFELLGIAPADAQERFGHLLEAFEYGAPPHGGIAPGIDRV
                     VALLAGVPNIREVIAFPKTQSAQDPMLGAPSPVPERQLADVHIRVVPPA"
BASE COUNT          429 a          795 c          879 g          439 t
ORIGIN      
        1 ccgccataga accgatcgtt cgcatccagg acgatgctct cgccgagctc ccattcgctg
       61 agcacaaagg ggcctgtgcc gttgggccgc cgcgtccagt tgcgcgggtt cgattcgatc
      121 tgctcgacgt cgacgacaaa cgccgtgggg taggtcagct ttgcgatgaa gtacggtttt
      181 ggcgcgtcga tcgttatctc gagcgtgtag tcgtcgatga cgcgaatgcc gctgatctcg
      241 tccgcgcggc cgcgcagctt gtcccgcacg cccacgatgt cgcccaggta gacgtcagcg
      301 accggcgatt gcgtctccgg ggacgcggcc cgctcgatgg aatccttgac cgtttgtgcg
      361 gtcacgggct tgccgctgtg gaagacgacg ttgcggcgta gatggaaggt atacaccgtg
      421 ccatcggcgc tcacgtccca gctctcggcg atgtcgggcg cgacttgcag gttctcgtcg
      481 atggtcacga gaccgctaaa gacctcgacg atgtagcgtg cggaatcggc gtccgtggca
      541 atgtgaggat cgagcgtggc ggggtcggcg cccaggagac ggagctcgtt gccctcgcgg
      601 tcgccgaggt cgggaccctc ctcggcggtc ggcgaggtgc cgccaccagc gtcgtcgtca
      661 tcgtctccgc cggagacgac aaggatggcg gcggcgccgc ccgcgagcgc gacagcggcg
      721 acggccgtgg ccagccacag caagagacga ttcatcggca accttccatc gatgtcatga
      781 gcgggggatc gtcatacaga cggggtcgac gcactcctcc acacggaatg gtacgcatca
      841 tagcagaggt cggttgtgtg gcgatgtcag gccggtggca cgacgcggat gtgaacgtcg
      901 gccagctggc gctcgggcac cggcgaggga gcgccgagca tggggtcctg ggcgctttgc
      961 gtcttcggga aggcgatgac ctcgcggatg ttggggacgc ctgcaaggag ggcgacgaca
     1021 cgatcgatgc caggagcgat gccgccgtgg ggcggtgcgc catactcgaa ggcctccagc
     1081 aggtgcccga agcgttcctg cgcatcggcc ggcgcaatcc ccagcagctc gaatatcgag
     1141 cgctgaagtt cgcgttcgtg gatgcggatg ctgccgctgc ccagctccca gccgttgcag
     1201 acgaggtcgt aggcgagggc gcgggcgcgg cccgggttgc tctcgaggaa gggcaggtcg
     1261 tcggggtggg gcgccgtgaa gggatggtgc acggcatccc agcggccttc atcgtcgttc
     1321 cactcgaaca tggggaagtc ggtgatgaag gcgaaggcca ggtggtgggg gtcggcgagg
     1381 cggaggcgct ccgccatctc gcgccgaagc acatcgagca ccgcgctcgt ccgccggtat
     1441 tcgtccgcaa ccatcaggag caggtcgccc cggccggctt cgcagcgccg ggccgcagcc
     1501 ctcacctcat cagccgagag gaagcgcgcc gccggagagc gaatatcctc gctggtgagg
     1561 ttctcgaggt cgccgtcccc gccaatcgcg agccaaacca ggcccttcgc tcccgccatc
     1621 tgcgcgatgg acgtcagttc gtccagctgg cgtcgcgggg tatcggcagc gccgcgcgca
     1681 gcgaacccgc gcacgcgccc gttcgactcc atggccgaac ggaacacgct gaactcggag
     1741 ccggcgaaca aatccgagag gtcatgaagc tccatgccgt agcgcacgtc gggcttgtcg
     1801 gagccatatc gctccatagc gtccgcatag gagatgcggg gaaacgtcga tggaacggag
     1861 aattgcggcg cgacctgacg gacgaggccc gcgaacatct cctccatcag gctcagcacg
     1921 tcgtcctgct cgacgaagct catctccaga tcgagttggg tgaactcggg ctgccggtcg
     1981 gcgcgcaggt cttcatcgcg atagcagcgg gcaatctggt agtaacgctc cactccggcc
     2041 accatgagca gttgcttgag ttgctggggc gattgcggca gggcatagaa ctccccgggg
     2101 tgcatcctgc tgggcaccag gaagtcacgc gccccttccg gggttgcctt gatgaggaca
     2161 ggcgtttcca cctccatgaa tcctcgttca tcgaggaagt cccggatgta ccggatggct
     2221 cggtggcgca gcgcgaggcg atcgtgcatg acggggcgcc gtagatctat gtagcggtac
     2281 ttgagacgca acagctcgtc gacttcttga tcctcgttga catagaaggg cgggttcttg
     2341 gccgggttca gaatctcgat gtccgccgcg tgcacctcga tctccccggt cgggagggat
     2401 gcgttctcgg tcccgggctt gcgcggccgc acgacgccgc gaacgcgcaa cacgtattcg
     2461 actcgcacgt cctcgccgat ggcgtgcgcg gccggcgcct cctgaggatt gatgacgacc
     2521 tgaatcagcc ctgtcgaatc gc
//
LOCUS       DATMBB010000367         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_140115, whole genome shotgun sequence.
ACCESSION   DATMBB010000367 DATMBB010000000
VERSION     DATMBB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140115"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VNL92_07870"
     CDS             <1..1194
                     /locus_tag="VNL92_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWO94666.1"
                     /translation="IARLIAAANAIERGELGQRVPDFDRTELGDLARAFNEMSRSLQA
                     AFSALAAERQRFVAVVSASTNGVIALDAARHIRYMNPSAEEILGMTREEAQGKPLLYA
                     LHQPEVHSRLTLVAEERRNEALSIELGPQRRWLRVLIMPLRDAGDWAFLIILVDLTEM
                     RRLETVRRDFVSNVSHELRTPLAALRAAIETLQEGALDDRPAAMSFLQRMLGEVDRLT
                     NLVDELLTLSRIEAQLNETLSREPVDIERVAGNVVERMRPQADRAGIRLSISGAAEGL
                     AVSGDPEQLDRAVTNLVHNALKFTPRGGSVVVATRREDGAAVLEVRDTGVGMSTADQA
                     RAFERFFKSDRSRSTEGAGLGLAIVRHVIQAHGGEVALESELGRGTTIRCLLPIAQGE
                     EATNS"
     gene            1326..1997
                     /gene="phoU"
                     /locus_tag="VNL92_07875"
     CDS             1326..1997
                     /gene="phoU"
                     /locus_tag="VNL92_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWO94667.1"
                     /translation="MTRGRYVQSLQQLHDEVLFLGALVTRAIERSTQALRTRDLELSQ
                     QVIEEDADINRKRFEIEDMILQLIATQAPMALDLRTMIAALLIVTDLERMGDHAEGNA
                     RLNRMMADLDECPEPDERIWRMAELATTMLGKALRSFVNHDAETAKLVCDQDDEVDRL
                     YDEHYRVIVERMVAGYPSLVTPLTYQLWTGHNLERIADRVTNICERVIFLVTGHMEEI
                     NVSRY"
BASE COUNT          471 a          774 c          769 g          411 t
ORIGIN      
        1 atcgctcggc tcatcgccgc cgccaacgcg atcgaacggg gcgagctggg ccaacgggtc
       61 ccagacttcg atagaacgga gctcggcgac ctcgcccgcg ccttcaacga gatgtcccgc
      121 tcgctccaag cggccttctc cgcgttggcc gccgagcggc agcggttcgt cgccgtagtg
      181 agcgccagca cgaacggggt catcgccctc gatgccgccc gtcacatccg ctacatgaat
      241 ccctccgccg aagagatact gggcatgacc cgggaggaag cgcaaggcaa acctctgctg
      301 tatgcgctgc accagccgga ggtgcactcg cgcctcacgc tcgtcgccga agaacggcgc
      361 aacgaagccc tgtcgatcga gctcggcccc cagcgtcgct ggctgcgcgt gctcatcatg
      421 cccctgcgcg atgcagggga ttgggcattc ctcatcatcc tcgtcgattt gacggagatg
      481 cgacggctgg agacggtccg ccgcgacttc gtcagcaacg tctcgcacga gctccggacg
      541 ccactggccg cattgcgggc cgcgatcgag acgctccagg agggcgccct cgatgaccgg
      601 ccagcggcga tgtcgttctt gcagcgcatg ctcggcgagg tcgaccgcct gacgaacctt
      661 gtcgatgagc tgctcacgct ctcacggatc gaggcgcagc tgaacgaaac gctctcgcgt
      721 gagccggtgg acatcgaacg agtggcgggc aacgtggtgg agcggatgcg gccgcaggcg
      781 gaccgggccg ggattcgcct ctcgatctcg ggcgccgccg aagggctcgc ggtgtcgggg
      841 gatccggagc agctggaccg tgccgtcacg aacctagtgc acaacgcgtt gaagttcacc
      901 ccgcggggcg gctctgtcgt tgtcgccacc agacgggagg atggtgcagc cgtcctcgag
      961 gtgcgggaca ccggagtggg aatgtcgacg gcggatcagg cccgcgcctt cgagcgcttc
     1021 ttcaagtccg accgatctcg ttcgaccgaa ggcgccggcc tagggctggc gatcgtgcgc
     1081 cacgtgatac aggcacacgg tggcgaagtg gccctggaga gcgagctggg ccgcggcacg
     1141 actattcgat gcctgctgcc gattgcccaa ggggaagaag caacgaattc ataacaatcg
     1201 cgtgaatcga gggcaaattc gccccgatcc cattgccaat cgttatagtt cagccgtata
     1261 cataacatac gcaattctgg cggcacgaag cccgagccat agagatactg ccaggggagg
     1321 acgccgtgac ccgcggcagg tacgtccaga gtcttcagca gctccacgac gaggtgctct
     1381 tcttgggcgc tctcgtgacg cgggcgatcg agcggtcgac gcaggccttg cgcacgcgcg
     1441 atctcgagct ctcccagcag gtcatcgaag aggatgcgga catcaaccgg aagcggttcg
     1501 agatcgagga tatgatcctc cagctcatcg ctacccaggc gcccatggct ctggacctgc
     1561 gcaccatgat cgccgcgctc ctcatcgtca cggacctgga gcgcatggga gaccatgccg
     1621 aaggcaacgc ccgcctcaac cggatgatgg ccgacctcga cgaatgcccg gagcccgacg
     1681 agcgcatctg gaggatggcc gagctggcca cgacgatgct cgggaaagcg ctccgatcct
     1741 tcgtcaacca cgatgccgaa acggccaagc tcgtttgcga ccaggacgac gaagtcgatc
     1801 gtctctatga tgagcactat cgggtgatcg tggagcgcat ggtcgctggg tacccttcgc
     1861 tggtcacgcc gctcacctat cagctgtgga ccgggcacaa cttggaacgt atcgccgacc
     1921 gcgtgaccaa catctgcgaa cgagtgatct tcctcgtgac gggccatatg gaagagatca
     1981 acgtttctcg ctactaacct tgagccgcat tgttatgctt ttgtaatccc actacccggg
     2041 gcgagggacg ctcccgcaac atcgagtccg gcatactgct ctcactggcc ggacgagagg
     2101 gtctcatgca agcgaccagg caaaagacag tttcgctcga agccatgcta agagcactct
     2161 tgaatccgga cggagcagtt tgcgtggccc gggcgagtgc agccgagcgg cgagccgcga
     2221 cgagcgctat aatgaacgcg taccagttgc ttcgcgaggg aaacgagaag ttgcaggacg
     2281 tgtgcgatcg attaggcgag gcggtgtttc tcagctatgc acgagaggca gtgacctagc
     2341 ccccctaaca ttgggtgccc cctttccccc ccgaagagga cccttgcgcc agcagcggtc
     2401 ctcttcggac gttaaggcat accgc
//
LOCUS       DATMBB010000368         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_574265, whole genome shotgun sequence.
ACCESSION   DATMBB010000368 DATMBB010000000
VERSION     DATMBB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574265"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="VNL92_07880"
     CDS             complement(<1..1340)
                     /locus_tag="VNL92_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWO94668.1"
                     /translation="MMRQDRFTEQAQEALASSQEMVRQQRHSQWDVEHVLAALLDHPA
                     GFARQILELAGVSPAAVQQRVAEALARSPKLAYDVVQIYTTPRIIRMLEAANAEAERL
                     HDEYVGIEHLLVAIADEREGEAARILRDSGIDKEKIYAALREIRGGARATGARAESRY
                     QALEKYSHDLTEAARQNKLDPVIGRETEVRRVMQILNRRTKNNPVLIGEAGVGKTAIV
                     EGLAQKIVADDVPENLRGKRLLALDMGALVAGSKFRGEFEERLQAVMDEIRRAEGEVI
                     IFIDEIHQVVGAGAAEGAIDASTMMKPALARGELRAIGATTLDEYRRYIEKDPALERR
                     FAPVYVGEPSVEDTIQILKGLRPRYEAHHKVQITDAALEAAAHLSDRYITERWLPDKA
                     IDLIDEASSKHVIDAQSMPDELRDLQRRVEQIGAEVDAAAERQDFEAAAKLRAIG"
     gene            complement(1468..1836)
                     /locus_tag="VNL92_07885"
     CDS             complement(1468..1836)
                     /locus_tag="VNL92_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWO94669.1"
                     /translation="MSTRDTRDRDDIDEEEPAYVISVAARMVGMHAQTLRYYERAGLI
                     RPSRSRGNIRLYSLADINRMRQIQRLINDLGVNLAGAEVILRMSDRISELEKENRELR
                     ANLQKFRDRRLPAPTPRDGS"
     gene            complement(1850..>2213)
                     /locus_tag="VNL92_07890"
     CDS             complement(1850..>2213)
                     /locus_tag="VNL92_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWO94670.1"
                     /translation="GPKGDLYLVVTVTPHARFERRGDDLLTDVDVPLVDAVLGGEVEV
                     PTIKGKRVMLTVPPLTQNGSTFRLAGQGMPRLGGSGHGDMYAKVRVQLPGRLSAEERE
                     LFERLRALQPQRKAAGVK"
BASE COUNT          303 a          756 c          730 g          424 t
ORIGIN      
        1 ccgatggcgc gcagcttggc cgcggcctcg aaatcctgcc gctccgccgc cgcatcgacc
       61 tcggcgccga tctgctcgac gcggcgctgc aggtcgcgca gctcgtccgg catgctctgc
      121 gcgtcgatga cgtgcttcga ggacgcctcg tcgatgaggt cgatcgcctt gtcgggcagc
      181 caccgctccg tgatgtagcg atcagagagg tgggcggccg cttcgagggc ggcatcggtg
      241 atctgcacct tgtggtgcgc ctcgtaccga ggacgcaggc ccttgaggat ctggatcgtg
      301 tcctccacgg atggctcgcc gacgtacacc ggggcgaagc ggcgctcgag cgccgggtcc
      361 ttctcgatgt agcggcggta ctcgtcgagt gtggtcgcgc cgatggcgcg cagctcgcca
      421 cgggcgagcg ccggcttcat catcgtgctg gcgtcgatcg cgccttcggc ggcgcccgcg
      481 cccaccacct ggtggatctc gtcgatgaag atgatgacct cgccctcggc gcgacggatc
      541 tcgtccatca ccgcctgcag tcgctcctcg aactcaccgc ggaacttgct cccggccacg
      601 agcgcgccca tatcgagcgc caacagccgc ttgccgcgca ggttctccgg cacgtcgtcg
      661 gccacaatct tctgggccag gccctcgacg atcgccgtct tgcccacgcc ggcctcgccg
      721 atgagcaccg gattgttctt cgtgcggcgg ttcaggatct gcatgacgcg ccgaacctcg
      781 gtctctcgac cgatgaccgg gtcgagcttg ttctgtcgcg ccgcctccgt caggtcgtgg
      841 ctgtacttct cgagcgcctg atagcggctc tcggcgcggg cgccggtggc gcgtgctccg
      901 ccgcggatct cgcgtagcgc cgcgtagatc ttctccttgt caatgccgga gtcacgcaga
      961 atgcgggcgg cctcgccctc gcgctcatcg gcgatggcga cgagcaggtg ctcgatgccg
     1021 acgtactcgt cgtggagccg ctcggcctct gcgttcgcgg cctccagcat gcggatgatg
     1081 cgcggcgtcg tgtagatctg caccacgtcg taggcaagct tcggcgagcg tgcaagcgcc
     1141 tccgcgacgc gctgttgaac cgccgcgggg ctcacgcccg cgagctcgag gatctgccgg
     1201 gcgaagccgg ccgggtgatc gagcagggcg gcgagcacgt gctccacatc ccactgagag
     1261 tggcgttgct gtcgcaccat ctcctggctg gaggcgagcg cctcttgcgc ctgctcggtg
     1321 aatcggtctt gtctcatcat ctgtctaccc tccctgggtg gccgatgcgc agttctctcc
     1381 cccatctgcc caagggcgca tcacaccttg ggcgggattc acctgccacc atgcgggtct
     1441 gcgatggttc aggcggttct ggctgagtca ggagccgtcg cgtggcgtcg gcgccggtaa
     1501 tcgacggtcg cggaacttct ggaggttcgc ccgaagctcg cggttttcct tctcgagctc
     1561 gctgatgcgg tccgacatgc ggaggatgac ctccgcccct gcgaggttga cccccaggtc
     1621 gttgatcaac cgctggatct gtcgcatgcg attgatatcg gccaacgaat agaggcggat
     1681 attgcctcgg gagcgcgatg ggcgaatgag gccggcgcgc tcgtagtacc gaagcgtctg
     1741 ggcgtgcatg cctaccatgc gcgccgcaac gctgatgacg tatgccggct cctcctcgtc
     1801 gatgtcgtcc cgatcgcgcg tgtcgcgggt gctcacgtca tcacctcgct cacttcactc
     1861 ctgctgcctt gcgttgcggt tggagcgccc ggagccgctc gaacagctcg cgctcctcgg
     1921 ccgaaagcct acctggcagc tgaacacgga ccttcgcgta catgtcaccg tgtccgctgc
     1981 cgcccagccg tggcatgcct tgcccggcca ggcggaacgt gctcccgttt tgggtcagcg
     2041 gaggcacggt gagcatcacg cgcttgccct tgatggtcgg aacctcgacc tcgccgccca
     2101 gaaccgcatc gacgagtgga acgtcgacgt ccgtcaggag gtcgtcgcca cggcgctcga
     2161 atcgagcgtg tggggtgacc gtgacgacga ggtagagatc gccttttggc ccg
//
LOCUS       DATMBB010000369         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_298440, whole genome shotgun sequence.
ACCESSION   DATMBB010000369 DATMBB010000000
VERSION     DATMBB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298440"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VNL92_07895"
     CDS             complement(<1..768)
                     /locus_tag="VNL92_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWO94671.1"
                     /translation="MKVAEIRKSFLRFFEERGHRVIPSSPLVPVGDPTLLFTTAGMVQ
                     FKPYFMGLAEPPARRMTSVQKCFRTTDIDEVGDASHLTFFEMLGNFSAGDYFKREAIA
                     YAWEYLTDVLAIPSDRLWITVFEDDDEAAALWREHGVEGARILRYTAKQGNYWYSGEV
                     GPCGPCSELHYDFGPVETCSACGAGTCHPALECGRFLEIWNLVFMAYFQHEDGRKEPL
                     PQANIDTGAGLERLARVLQQGPSVYETDVFLPVIERIE"
     gene            863..937
                     /locus_tag="VNL92_07900"
     tRNA            863..937
                     /locus_tag="VNL92_07900"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:895..897,aa:Ile,seq:cat)
     gene            complement(980..1822)
                     /locus_tag="VNL92_07905"
     CDS             complement(980..1822)
                     /locus_tag="VNL92_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO94672.1"
                     /translation="MRAESRFAQLGQIRMHYWDWGGTGQPVLFVHANGFHGAVWSPIA
                     EALLPEYRSIAVDVRGHGDSDKPDQGYEWTQLASDIGELLHALDVRDAVGIGHSLGGG
                     LTVICAALYPGAISRAVLIDPVITSGPPTSEGVPINPLAEAARKRRHRWASKREAFEH
                     LRTRATFAGWREDVLWLYVDYGLRDSPEGGVELKCSGEIEARIFEETARSPAFSMLGA
                     LEGHSLILLGEASYVGEAIARGAAGRMRSVELDYVPGTTHFLVMERPDDVASRIRAFL
                     EQRV"
     gene            complement(1871..>2204)
                     /locus_tag="VNL92_07910"
     CDS             complement(1871..>2204)
                     /locus_tag="VNL92_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWO94673.1"
                     /translation="VIVGHSFGGAVAISAAARDRRIAAVVGLSNQTAGAQPVGRIAPR
                     PLLLVHGLDDDRLPPSLSRTIYAWAGEPKGLVLLAGARHSLRQRREDLRAILIRWLTE
                     ALRAPRQP"
BASE COUNT          385 a          714 c          751 g          354 t
ORIGIN      
        1 ctcgatgcgc tcgatgacgg ggagaaagac atcggtctcg tacaccgaag gcccttgttg
       61 gagcacgcgg gcaaggcgtt cgaggccggc acccgtatcg atgttcgcct ggggcagcgg
      121 ctccttcctg ccgtcctcgt gctggaagta cgccatgaac acgaggttcc atatctcaag
      181 gaagcgcccg cattcgaggg ccggatgaca tgtcccggcg ccgcaggcgg agcacgtctc
      241 gacggggccg aagtcgtagt gcagctcgct gcaggggccg cagggtccca cctcgcccga
      301 gtaccagtag ttcccttgct tcgccgtgta ccggaggatg cgggccccct cgacgccgtg
      361 ctcgcgccag agggcggccg cctcatcgtc gtcctcgaac acggtgatcc acagccggtc
      421 ggacgggatc gcgaggacgt cggtcagata ctcccacgcg taggcgatcg cctcgcgctt
      481 gaagtaatcg ccggcgctga agttgccgag catctcgaag aacgtgaggt ggctcgcgtc
      541 gcccacctcg tcgatgtccg tggtccgaaa gcacttctga accgaagtca tgcggcgagc
      601 tggcggctcg gccagtccca tgaagtacgg cttgaactgc accatgcccg cggtggtgaa
      661 cagcagcgtc gggtcgccga cggggacgag gggggagcta gggataacgc ggtgcccgcg
      721 ttcctcaaag aatcgaagga acgatttgcg aatctcggcg accttcaatg gggcctcaaa
      781 gtcgatgcgt aatggacggg agaagtatag catgaacgtt cggctcgccc gccgggacgt
      841 ggtacgatga cgagtgaaat ccgggcccgt agctcaacgg cagagcggtc ggctcataac
      901 cgactggttg caggttcgag tcctgccggg cccaccatcg acggatacgg attcccctcg
      961 atccggctgg ccaccgcgcc tacacacgct gctcgagaaa ggcccggatg cgcgatgcga
     1021 cgtcgtccgg acgctccatc acgaggaagt gcgtcgtgcc cggcacgtag tccagctcca
     1081 ccgagcgcat tcggcccgcc gctccacggg cgatagcctc gccgacgtag ctcgcctcgc
     1141 cgagcagaat gaggctatgt ccctcgagag cgccaagcat cgagaaggca ggagaccgtg
     1201 ccgtctcctc gaagatccgc gcttcgatct ccccgctgca cttcagttcg accccgccct
     1261 cgggcgaatc gcgcaggccg tagtcgacgt acagccacag cacatcttcg cgccatccgg
     1321 cgaacgtcgc ccgcgtacgg aggtgctcga atgcctcccg cttgctcgcc cagcggtggc
     1381 gccgtttgcg cgccgcttcg gccagtgggt tgatgggcac accctcggac gtgggcggac
     1441 cggacgtgat gacgggatcg atgaggaccg cgcgcgagat ggcgccgggg tacagtgcgg
     1501 cgcagatgac cgtgagtccg ccgccgaggg aatgcccgat gccaaccgcg tcgcgaacat
     1561 cgagggcgtg gagcagctct ccgatgtcgg aggcgagctg ggtccactca tagccctgat
     1621 ccggcttgtc ggagtcgccg tggccgcgca cgtcgaccgc gatcgatcgg tattcgggaa
     1681 gcagtgcctc ggcgatcggc gaccatacgg cgccgtggaa cccgttcgca tgaacgaaga
     1741 ggaccggctg gcccgtcccg ccccaatccc agtagtgcat ccgaatctgg cccagttggg
     1801 cgaagcggct ctcagcgcgc acgtcgtcct ccgtctgccc gcggcatcgt aggcttcgat
     1861 gttcgagggg tcaaggttgg cgcggcgcac gaagcgcctc ggtgagccag cgaatgagga
     1921 tggcgcggag gtcctcgcga cgttggcgca ggctatggcg ggcaccggcg agcagcacga
     1981 ggcccttcgg ttcgcccgcc cacgcgtaaa tcgtgcgtga cagtgagggt ggaaggcgat
     2041 cgtcgtcgag cccgtgaacc aggagcaggg gccgaggcgc tatgcggcct acgggctggg
     2101 cgcccgccgt ctgattcgag aggcctacca cggccgcgat tcgtcggtcg cgcgcggcgg
     2161 cgctgatggc aaccgctccg ccgaatgaat gtcccacgat gacg
//
LOCUS       DATMBB010000370         4750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_377411, whole genome shotgun sequence.
ACCESSION   DATMBB010000370 DATMBB010000000
VERSION     DATMBB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377411"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VNL92_07915"
     CDS             complement(<1..780)
                     /locus_tag="VNL92_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HWO94674.1"
                     /translation="MTLGTSGCYDPRNSIVRGALLVRFFRRGSGQPAAEAEAEQARRQ
                     TQQATERTRSGWFGRMAGIFSRSKIDEELWEELEEILLGSDAGLETTERLLSELRDRV
                     ERDGIREPTEVEELLKQQLVDLLQQQESHGGKLWSEGATEVAQPAVILVVGVNGVGKT
                     TTIGKLAHKYVQEGASVIVAAGDTFRAAAIEQLREWGERVGAAVVAHKTGADPGAVAF
                     DALEAAEARQADLVIIDTAGRLHTKYNLMEELRKVRRIVERK"
     gene            complement(797..4423)
                     /gene="smc"
                     /locus_tag="VNL92_07920"
     CDS             complement(797..4423)
                     /gene="smc"
                     /locus_tag="VNL92_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWO94675.1"
                     /translation="MYLKRLELQGFKSFAGKTLIEFGPGVTAIVGPNGSGKSNIVDAM
                     RWVLGEQSARTVRAKRTDDLLFAGSSKRPALGLAEVNLILDNSESWLPIDFSEVVITR
                     RAYRSGESDYLLNRNQVRLRDVVDLLLKGNVAQDSYAILGQGAVETALGLRPEERRVL
                     IEEAAGVRHYRARLDESRDRLAATRENLERVQMLIAEIAPRLGHLERQARRAEESARL
                     TRELSEALRAWYGYQWQAVYGALAAARARHDQAAATYHEAQERGAHDERRLSDLRAAL
                     EALRSDIAAAEEAHRALTLEAREIDRRGAVASERRSLLTVRREEIEREIAALAEECAE
                     QSSLLAEDVERRQALTEDLARSQAELAERQRALEDFETRWRDARTVAAEAQEHAARAA
                     AAASDLERRIEQMGAERERLAAETARLDERKSDLDIRLADIGDSEARLEDEEERISRD
                     LAEVLRQHDALKRLISEGQPAVAALEERYQESSRALAAAESRYEAIRAAQEEHEGADI
                     AQQKLFSPDTELQGIIGTLTGLLQVPRGMERAIEAGLADSLRAVVVDRPENAYAIVQE
                     LQAEGHGNVTIIPLQGLREVSPLNLHKEKGIVGVASRLVRCDSQYRKLVDSLLGRVIV
                     VEDIRIGREVLRRGLGSVVTMDGVLLRPLGSFTLAPTSVGVSPFDRKAQLRELPERIE
                     QLRAELDSVKAELTAKRQAIEANESALAMIARKTDALRTARADKLDEVTAVRGKLAQL
                     RGEMRWIVEAQHQNRERLVALDQAARELTVEREALLSTATETATVSERQGELIAALGQ
                     ERDALLQDAAEASARVTSLEGELRSIATLRAKQEATVARLEEQLSGKREQLVRLTEEL
                     ATMESEVEAAAEREATLREQVGATGHAVEEMQARRSGLEEEMSRARESYARAREALVQ
                     AERDQREAAAEVERREAEVAALRDTLRDEGIELPELEADMVAAAAGGQEAVPEYLVRK
                     EESRPPVAGAAQVEDPAKLKAHIGELRAAIRALGPVNAEAVADYEEGRERHDFLTGQV
                     RDLQESEASLRSAIGELEDIIRTQFKETYEQVGEAFSSYFTAFFGGGKAELVLTPPED
                     HAASGIDIVVEPPGKKLQSMALLSGGERSLTALALLFALLKVRAVPFVVLDEVDAALD
                     EGNVDRFAQALTDLTSHTQFIVVTHNRRTIEAADAIYGVTMGGDGVSRVLSLRLADLP
                     ANLT"
     gene            complement(4428..>4750)
                     /locus_tag="VNL92_07925"
     CDS             complement(4428..>4750)
                     /locus_tag="VNL92_07925"
                     /inference="COORDINATES: protein motif:HMM:NF026059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HWO94676.1"
                     /translation="ERFVLRQLGGRLRALDVEALLDPKSRLQHLTQARWHVAPQYTLV
                     AAQGPDHERRFTVQVDVAGEVMAQGAGASKQDAERIAAERALATLADRGIAHEPGDGT
                     VGAT"
BASE COUNT          733 a         1624 c         1484 g          909 t
ORIGIN      
        1 cttccgctca acgatgcgcc gcaccttgcg gagctcctcc atgaggttgt acttggtatg
       61 gagcctgccc gcagtatcga tgatgacgag atctgcttgc cgcgcttcgg cggcctcgag
      121 cgcatcgaag gcgaccgctc cgggatctgc gcccgtcttg tgtgccacca cagctgcacc
      181 cacccgctcg ccccactccc gcaactgctc gatagcggcg gcgcggaacg tatctcctgc
      241 ggccacaatg acggaggcgc cttcctgtac atacttgtgc gccagttttc cgatggttgt
      301 cgtcttccca acgccattca cgcccacgac gaggatgacg gctggctgcg cgacctccgt
      361 cgcgccttcg ctccagagct tgcccccgtg cgactcttgc tgctggagca agtcgacgag
      421 ttgctgtttg aggagctcct ccacttcggt cggctcacga attccgtcgc gctcgacgcg
      481 gtcgcgcagc tcgctcaaca ggcgctccgt cgtctcgagc cctgcatcgg agccgagaag
      541 gatttcctcg agttcttccc acagctcttc gtcaatcttg gaacgtgaga agatgccggc
      601 catgcgtcca aaccagcccg aacgagtccg ctcggtcgcc tgctgggttt ggcggcgcgc
      661 ttgttcggct tcggcctcgg cggccggctg cccggagccc cggcgaaaga agcgtaccag
      721 gagtgcacct cggacaatcg aatttcgcgg atcgtagcat ccgctcgttc cgagggtcaa
      781 cgaaagtcgg gtcgggtcag gtcaggttgg cgggaaggtc cgcgaggcgg agggagagga
      841 cacgcgatac tccatcccct cccatggtca cgccgtagat tgcatcggcg gcttcgatgg
      901 tacggcgatt gtgcgtcacg acgatgaact gcgtgtggct cgtgaggtcg gtgagggctt
      961 gagcaaagcg atccacgttg ccctcgtcca atgccgcgtc gacctcgtcg agcacgacga
     1021 agggcacggc acgaaccttg agcaaggcga ataggagcgc gagggccgtg agcgaccgct
     1081 cgccgcccga aaggagcgcc atactctgca gcttcttgcc gggcggctcg acgacgatgt
     1141 cgatgcccga cgctgcatgg tcctcgggcg gtgtcaggac gagctcagcc ttgccgcccc
     1201 cgaagaatgc ggtgaagtag ctcgagaagg cctccccaac ctgctcgtag gtctccttga
     1261 actgggttcg gatgatgtcc tcaagctctc cgatagcgct ccgcaaggac gcttctgatt
     1321 cctgaaggtc gcggacttgg ccggtgagga aatcgtgtcg ctctcgacct tcctcgtagt
     1381 cggccacggc ctccgcgttt acgggaccaa gggcgcgaat ggctgcgcgc agttcgccaa
     1441 tgtgggcctt cagcttcgcg gggtcctcga cttgagcagc gcccgcgacg ggaggccggc
     1501 tctcttcctt gcgaacgagg tactctggta ccgcctcctg accgcccgca gcggcagcca
     1561 ccatgtcggc ctcgagctcg ggaagctcga tgccctcatc gcgcagcgta tcgcgaagcg
     1621 cggcaacctc cgcctcgcgc cgttcgacct ccgcggccgc ctcgcgctgg tctcgctccg
     1681 cctgcaccag cgcttcccgg gcacgggcgt aggattcgcg tgcccgcgac atctcctcct
     1741 ccaatccgga gcggcgcgcc tgcatctcct caacggcatg tccggtggcg ccaacctgct
     1801 cgcgtaacgt ggcttcgcgt tcggctgccg cttcgacctc gctctccatg gtggcgagct
     1861 cttcggtcag tcgaaccagc tgctcgcgct tcccagatag ctgctcctcc agccttgcca
     1921 cggtagcttc ctgcttcgcg cggagcgtgg ctatggagcg gagctcacct tcgagtgaag
     1981 tgacgcgagc agaggcctcg gcggcatcct ggagcagcgc gtcgcgctct tgcccgagcg
     2041 cggcgatgag ctcgccttgc cgctccgaca cagttgcagt ctcggtggcg gtgctgagga
     2101 gcgcctctcg ttctaccgtc aattcgcggg cggcctgatc cagcgctacg agcctctcgc
     2161 ggttctggtg ctgagcctcg acgatccacc gcatctcgcc gcgcaattgc gcaagcttgc
     2221 cgcgaacggc ggtgacctca tcgagcttgt cggccctggc cgtgcggagc gcgtccgtct
     2281 tgcgcgcaat catcgcgagc gctgattcgt tcgcctcgat ggcctgacgc ttcgcggtca
     2341 gctcagcttt cacgctgtcg agttcggcgc gcagctgctc gatacgctca ggaagctcgc
     2401 gcaattgtgc cttccgatcg aacgggctga cgcctacgga cgtcggtgcc agcgtgaatg
     2461 accctagcgg ccgaagcaac acgccgtcca tcgtcacgac cgacccgagg cctcggcgca
     2521 gcacctcgcg tccgatgcga atgtcctcga cgacgatgac ccgtccaagc agcgaatcga
     2581 ccagctttcg atactgcgaa tcgcaccgga cgagccgaga ggcaacaccg acaatgccct
     2641 tctccttgtg gaggttgagc ggagaaacct cgcgcagtcc ctggagtggg atgatggtca
     2701 cattcccatg accttccgcc tgtagctcct ggacgatggc ataggcgttc tctggcctgt
     2761 ccaccacgac ggcgcgaagg ctgtcggcca gtcccgcctc gatggctcgc tccatccccc
     2821 ttggcacttg gagaaggcca gtgagagtcc cgatgatgcc ctgcaactcg gtgtcgggac
     2881 tgaatagctt ctgctgggct atatcggcgc cctcgtgctc ctcctgggcg gctcggatcg
     2941 cctcatagcg gctctccgcc gcggcaagcg cacgggatga ttcctggtag cgctcctcaa
     3001 gcgcggcgac ggcaggctgc ccttcggaaa tgaggcgctt gagtgcgtcg tgctgacgaa
     3061 gcacctccgc caagtccctg cttattcgct cctcttcgtc ctcaagacgc gcctcgctgt
     3121 cgccaatatc cgccaggcgg atatcgaggt cagactttcg ctcatcgagg cgggcggtct
     3181 ccgcggcgag cctctcgcgt tcggcgccca tctgctcgat gcggcgctcg aggtcggacg
     3241 ctgcagcagc tgctcgggca gcatgttcct gggcctcggc ggccacggta cgcgcgtcac
     3301 gccacctcgt ctcgaagtcc tcgagagcac gctggcgctc agccaactcc gcctggctcc
     3361 gcgcgagatc ttccgtgaga gcctgccggc gttcgacatc ctccgcgagc aggctgcttt
     3421 gttcggcaca ttcctccgcg agcgcagcga tctcgcgctc gatctcctcc cggcgcaccg
     3481 tgagcaacga gcggcgctcg cttgcgaccg cgccacgccg gtcgatctcg cgggcctcga
     3541 gcgtcagtgc gcgatgcgct tcctcggcag ccgcaatatc ggagcgaagg gcttcgagcg
     3601 cggcgcgcaa atccgagagg cggcgctcgt catgggcgcc tctctcttgc gcttcgtgat
     3661 atgtggctgc agcctggtcg tgtcgagcac gagcggcggc cagggctcca tagacggcct
     3721 gccactggta tccataccag gctcgtagcg cctcggacag ctctcgcgtc aggcgcgcgc
     3781 tctcttctgc ccggcgggct tggcgctcga ggtgtccgag tcgtggcgcg atctcggcga
     3841 tgagcatctg cacgcgctcg aggttctcac gtgtcgccgc aagccgatcg cgcgactcgt
     3901 cgagacgagc gcgatagtga cgcacgcccg ccgcctcctc gatgaggacg cgacgttcct
     3961 ccggtcggag tccgagcgcc gtctccacgg caccctgtcc gaggatggcg tagctgtcct
     4021 gcgcaacgtt ccccttgagg agcaggtcca caacgtcgcg cagacgcacc tggttccgat
     4081 tcaacagata gtcggactcg cccgatcgat aggcgcgtcg ggtgatcacg acctcagaga
     4141 agtcgatggg taaccaggat tcggaattgt cgaggatgag gttcacctcg gcaagtccga
     4201 gcgctggtcg cttggagctt cccgcaaaga ggagatcgtc cgtacgcttc gcccttaccg
     4261 tgcgggcgct ctgttctccg agcacccagc gcatcgcatc gacgatgttg ctcttgccgc
     4321 ttccgtttgg cccgacgata gccgtgactc ccggcccaaa ctcgatgagc gtcttgccgg
     4381 cgaagctctt gaacccctgc agttcgagtc gcttgaggta catctgacta tgtagctccc
     4441 acggtgccat cgcccggctc atgcgcgatg ccgcgatcgg cgagggtggc gagcgcacgt
     4501 tccgccgcga tacgctcggc gtcctgtttc gaggctccgg cgccttgcgc catcacctcg
     4561 ccggctacgt cgacctgcac cgtgaatcgt cgctcatgat ccggcccctg agcagccacg
     4621 agcgtgtact gcggagcgac gtgccagcgc gcttgtgtca ggtgctgcaa tcgcgacttc
     4681 gggtcgagga gggcttcgac atccagcgct cggaggcggc cgcccagctg ccgcagcacg
     4741 aagcgctcgg
//
LOCUS       DATMBB010000371         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_415934, whole genome shotgun sequence.
ACCESSION   DATMBB010000371 DATMBB010000000
VERSION     DATMBB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415934"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VNL92_07930"
     CDS             complement(<1..566)
                     /locus_tag="VNL92_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWO94677.1"
                     /translation="MTGAARSLSFAEFDRLFDEVKNWGRWGAEDERGALNLITAEKRR
                     AAASLVRDGIAVSCALPLATQPGPENPSPVVHLMAGAGDQPNTYASFDYFAIAPHGMA
                     NTHLDALCHIFYRGQMYNGFPCTQVTSTGALACSIEAAEDGVVSRGVLLDVARTVGKP
                     WLEPGEAIYVEDLEAAERRAGVRVEEGD"
     gene            complement(568..2157)
                     /locus_tag="VNL92_07935"
     CDS             complement(568..2157)
                     /locus_tag="VNL92_07935"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWO94678.1"
                     /translation="MDVPLLVDDFLRRAAKLYPNKEAVVDGELRYTYGQFDERVNRMA
                     NALAAMGIAKGDRVCILSPNSHFFLESFYAMPRLGAILVPVNYRLVASDHEYIMNHAG
                     VKAVLADHAYTHLVHEVRGNLAHCERFLVLQDGGPPQPGWEDMEELMRVASPERPPDC
                     GADERDVVTINYTSGTTARPKGVMLTHRNCYINAYNFIAHLRISHDDVELWTLPMFHA
                     NGWGGPFAITAMAGKHVVLRAVVAEDIYRLIEEEGVTFACMAPAVLSTILNYERKAEH
                     RFTTRPRFVVAGTPPPASFIERLEQEIGWEFIQIYGLTETAPILTVSVPDHQLAAASG
                     ADPYRRRARAGVEAIGVDLQVLDDEGRPVPHDDTTVGEVCARSNVVMQGYWQQQAETD
                     KVIYDGYFHTGDLATWDEFGNINIVDRKKDVIISGGENISSAEVEDALYKHPAVLECA
                     VIGVPSERWGETPKALIVLREGASATEEEIIGFARERLAHFKCPSSVDFVESLPRTAT
                     GKLQKFILRERFWEGIDRRVH"
     gene            2387..>2577
                     /locus_tag="VNL92_07940"
     CDS             2387..>2577
                     /locus_tag="VNL92_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HWO94679.1"
                     /translation="MPFVDRTLTCQNCGSSFTFTAEDQEFFEQKGFTEPRRCMPCRQQ
                     RRAERGESSYSPRTSERRE"
BASE COUNT          449 a          826 c          835 g          467 t
ORIGIN      
        1 atgtcgcctt cttcgacacg aacgcctgcc cgccgctcgg ccgcctccag gtcctcgaca
       61 tagatcgctt cgcccggctc gagccacggc ttcccgaccg tccgcgcgac atcgagaagc
      121 acgccgcgac tcacgacgcc atcctccgcc gcctcgatag agcaggcgag cgccccggtc
      181 gaggtgacct gggtgcaggg gaagccgttg tacatctggc cgcggtagaa gatgtggcaa
      241 agcgcatcga ggtgcgtgtt cgccataccg tgcggggcga tggcgaagta gtcgaacgag
      301 gcatacgtgt tcggctgatc gccggccccg gccatgaggt ggactacggg actggggttc
      361 tcgggaccgg gctgggtcgc gagcgggagc gcgcaactga cggcgatacc atcgcgcacc
      421 aggctagccg cggcgcgccg cttttcggcc gtgatgagat tgagcgcgcc gcgctcgtcc
      481 tcggcgcccc agcgccccca attcttgacc tcatcaaaca ggcggtcgaa ctcagcgaag
      541 ctcaggctgc gcgctgctcc tgtcatgtca gtgcaccctc cggtcgatgc cctcccagaa
      601 ccgctcccgg aggatgaact tctgcagctt acccgtcgcc gtccgcggca aagattcgac
      661 gaaatcaacc gacgaggggc acttgaagtg ggcgagccgc tcgcgggcga atccgatgat
      721 ctcctcttcc gtcgcactcg cgccctcgcg caacacgatg agggccttcg gagtctcgcc
      781 ccaccgctcg ctcgggacgc cgatcacggc gcactccagc acggccgggt gcttgtagag
      841 cgcgtcctcc acctctgccg acgagatgtt ctcgccgccg gagataatca cgtccttctt
      901 gcgatcgacg atgttgatgt tgccgaactc gtcccaggta gcgaggtcgc cagtgtggaa
      961 atagccgtcg tagatcacct tgtcggtctc ggcctgctgc tgccagtacc cctgcatcac
     1021 gacattcgag cgcgcacaca cctcgccgac ggtcgtatcg tcgtggggta ccggccggcc
     1081 ctcgtcgtcc agcacctgca ggtcgacacc gatcgcttcc accccggccc gggcgcggcg
     1141 gcgatatgga tcggcgccgg acgctgcggc gagctggtga tcgggcacgc ttacggtgag
     1201 gatgggggcc gtttccgtga gaccgtagat ctggatgaac tcccagccga tctcctgttc
     1261 gagccgctca atgaaggagg cgggcggagg ggtgccggcc acgacgaatc ggggccgcgt
     1321 cgtgaagcga tgctccgcct tgcgttcgta gttgagaata gtgctcagca ccgccggcgc
     1381 catgcaggcg aaggtcacgc cttcctcctc gatcaggcgg tagatgtcct cggccacaac
     1441 ggcgcgcagg acgacatgct tgccggccat cgcagtgatg gcgaaggggc cgccccatcc
     1501 gttggcgtgg aacatgggaa gcgtccagag ctcgacgtcg tcgtggctga tgcgcaagtg
     1561 ggcgatgaag ttgtacgcat tgatgtagca gttgcggtgc gtgagcatga cgcccttcgg
     1621 ccgcgccgtg gtgcccgacg tgtagttgat ggtgacgaca tcgcgctcgt ccgcaccgca
     1681 gtccggggga cgctcgggcg aggccacgcg catcagctcc tccatgtcct cccacccggg
     1741 ttggggtggc ccgccatcct gcagcacgag aaagcgctcg caatgagcaa ggttcccacg
     1801 cacctcatgg acgagatgtg tatatgcgtg gtcggccagc acggccttca cgccggcatg
     1861 gttcatgatg tactcgtggt cggaggcgac caggcggtag ttgaccggca caaggatggc
     1921 gccgagccgg ggcatcgcgt agaagctctc gaggaagaag tgcgagttcg ggctcaggat
     1981 gcagactcga tcgcccttgg cgatgcccat cgcggcgagg gcgttcgcca tccggttgac
     2041 ccgctcgtcg aactgcccat aggtgtaccg aagctcgcca tcgacgacgg cctccttatt
     2101 ggggtagagc ttcgctgcgc ggcgcaggaa atcgtcgacc agaagtggaa cgtccatcgt
     2161 ctcccccctg aggtccgacc aatcgtcggt ttggcggatc gcgatgcgat gatagcgcga
     2221 ccgtcaagaa gcgaagcatc gacttcgctc caagccttcc gctggaagct acgatagcgt
     2281 atagtagtaa gtgagccggc caaggccggc gagcgttatc gttaagctgc tcctcgtcac
     2341 tggcctcggc accgacgact ggacagcaca ttatggaggt cgttgcgtgc cctttgtcga
     2401 tcgtaccctt acctgtcaaa actgtggttc tagctttacc tttactgccg aagatcagga
     2461 gttcttcgag cagaaggggt tcaccgagcc caggcgttgc atgccctgtc gtcagcagcg
     2521 ccgagcggag cgaggcgaat cgtcgtactc gccgcggacg tcggagcgcc gcgagat
//
LOCUS       DATMBB010000372         2402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_139815, whole genome shotgun sequence.
ACCESSION   DATMBB010000372 DATMBB010000000
VERSION     DATMBB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139815"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1367)
                     /locus_tag="VNL92_07945"
     CDS             complement(66..1367)
                     /locus_tag="VNL92_07945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWO94680.1"
                     /translation="MKFGIFYEHQLPRPWSEGRELKLFQDALSQVELADKLGIDHAWE
                     VEHHFLEEYSHSSAPEVFLAAASQRTKNIRLGHGIVLMPPNYNHPARIAERIATLDLV
                     SNGRVEWGTGESASRAELEGFRIDPNQRRAMWRETVEQVANMLAMDPYPGFEGEFFSM
                     PTRNVVPKPVQKPHPPIWVACSNRDTIHLAAQLGIGALTFAFVDPAEAQHWVNDYYET
                     FKRECVPIGHSVNPNIAMVTSFSVHEDEEEARRRGLDGFRFFQFALGHHYNFGQHKPG
                     RTNIWQHYLAVRDAMGMDMMGGGTGGIGTPEQQRQHLRALADAGVDQAVFIQQGGNNQ
                     HEHICEALELFAAKVMPEFKDEESARLRRKEQELAPYVEAALKRKEYMREFSDDEIPV
                     YPAYGFNVAEIDLASLPEAQRRRLEQMRRMREVIERVQVEV"
     gene            1546..>2402
                     /locus_tag="VNL92_07950"
     CDS             1546..>2402
                     /locus_tag="VNL92_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO94681.1"
                     /translation="MQFRFTPEQESFRKEIRAFLREAGEAASIEAQGGRWERYRAFQK
                     RMAERGWLTLAWPKEYGGQAADYMTQLVFNEEMSYHGAPNPGIGVDRVGPTMMIYGTE
                     EQKAFHLPRIAAGEVIWCQGFTEPQAGSDLASLQTRAVLDGDSFVINGQKMFTSLAHA
                     ADYCALLARTDPEAPKHRGISYFLLDMRTPGITVRPLLDAMGNHTFNEVFLEDVRVPR
                     SSVLGEVNRGWYLASTTLDFERSGIHRVMGSFRQFEMLVDFCRAEHGDASALIQRPSV
                     RHRLADLKIA"
BASE COUNT          413 a          805 c          742 g          442 t
ORIGIN      
        1 tggccaaccc cttgggttgg ccatcatcgc gccatcgata cggcgtgccc agcgatcgat
       61 gtccgtcaca cctccacttg cacccgctcg atgacctcgc gcatgcggcg catctgctcc
      121 agccggcggc gctgcgcttc gggcaatgac gctaggtcga tctcagcaac gttgaagccg
      181 tacgccgggt atacaggaat ctcgtcgtcg gagaactccc gcatgtattc cttacgcttg
      241 agcgcggctt ctacataggg cgccaactct tgctccttgc gccgaagccg cgccgactcc
      301 tcatctttga actcaggcat caccttggcg gcgaacagct ccagcgcctc gcagatgtgc
      361 tcgtgctgat tgttgccgcc ctgctggata aacacggcct ggtccacgcc ggcatccgca
      421 agcgcccgca ggtgctgccg ctgctgctcc ggcgtgccga tgcctcccgt gccgccgccc
      481 atcatgtcca tccccatcgc gtcgcgcacg gcgaggtagt gctgccagat gttggttcgg
      541 cccggcttgt gctgcccgaa gttgtagtga tgccccaacg cgaattggaa gaagcggaag
      601 ccgtcgagtc cccgacgccg cgcctcctcc tcgtcctcgt gcacagagaa gctcgtgacc
      661 atggcgatgt ttggattgac gctgtggcct atgggcacgc actctcgctt gaaggtctcg
      721 tagtagtcgt tcacccagtg ctgcgcctcc gctggatcga cgaaggcgaa ggtgagggcg
      781 ccgatgccga gctgggcggc gaggtgaatc gtgtcccggt tcgaacaggc aacccagatc
      841 ggcgggtgcg gcttctgcac aggcttcggc acgacgttcc gggtgggcat ggagaagaac
      901 tcgccctcga accccgggta cgggtccatg gccagcatgt tcgcaacctg ctcgaccgtc
      961 tccctccaca tcgcccggcg ctggttcggg tcgattcgga acccctcgag ctccgcgcgg
     1021 ctggccgatt cgccggtacc ccattcgacc cggccgttcg agacgagatc gagcgtggcg
     1081 atgcgctcgg cgatgcgcgc cggatgattg tagttgggcg gcatgagcac gatgccgtgg
     1141 cccaagcgga tgttctttgt ccgctgcgag gcggcggcga ggaagacctc gggcgcagag
     1201 gagtgcgagt actcctcgag aaagtggtgc tccacctccc acgcgtggtc gatgccgagt
     1261 ttgtcggcga gctccacctg cgagagcgcg tcctggaata gcttcagctc gcgcccctcc
     1321 gaccacggcc gaggcagttg atgctcatag aatatcccga acttcacttc gatcctcccc
     1381 cacgtgatga gccgcgcaag atgtgccgcg gttgcctcac cataccacag aaaacgggga
     1441 cttggtgctg cgaaagcgca tcattctggt tccatcgctg catcgcgccg ggaaggaagc
     1501 gttcggagta tactgctccc gaattcgcac gagagtcgat tcgagatgca gttccggttc
     1561 actcccgaac aagagtcctt ccgcaaggag attcgcgcct tcctccgcga ggccggcgag
     1621 gccgcgtcca tcgaagcgca aggcggccgc tgggagcggt accgtgcgtt ccagaagcgg
     1681 atggccgagc gaggctggct cacgctggcc tggccgaagg aatacggtgg ccaagcggcc
     1741 gactacatga cgcagctcgt gttcaacgag gagatgtcgt atcacggcgc gccgaatccg
     1801 gggatcggcg tcgaccgggt ggggcccacc atgatgatct acggcacgga ggagcagaag
     1861 gccttccact tgccgcggat tgccgccggc gaggtcatct ggtgccaggg attcaccgaa
     1921 ccgcaggcag gctccgacct cgcctccctg cagacccgcg ctgtcctcga tggcgacagt
     1981 ttcgtcatca acgggcagaa gatgttcacc tccctcgccc acgcggccga ctactgcgcc
     2041 ctgctggcgc gcacggaccc cgaagcgccg aagcaccgag gcatctcgta cttcctgctc
     2101 gacatgagga cgcccggaat caccgtgcgg ccgcttctgg acgcgatggg caaccacacc
     2161 ttcaacgaag tgttcctcga ggacgtgcga gttcctcgta gctctgtcct cggcgaggtc
     2221 aaccgcggct ggtatctggc atccacgacc ctcgacttcg aacgatcggg catccaccgc
     2281 gtcatgggat cgttccgcca attcgagatg ctggtcgact tctgccgcgc ggaacacggc
     2341 gatgcctcgg ccctcatcca gcggccatcg gtccgccacc gcctcgccga cctcaagatc
     2401 gc
//
LOCUS       DATMBB010000373         2408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_178342, whole genome shotgun sequence.
ACCESSION   DATMBB010000373 DATMBB010000000
VERSION     DATMBB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178342"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VNL92_07955"
     CDS             <1..969
                     /locus_tag="VNL92_07955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO94682.1"
                     /translation="IGDKLGLYKALHELGPATPGELAARTHTSERYLRHWLLNQAASG
                     YITYSAATGKYSLSPEQAMVFADDDSPAAMAGGFGLMTSLVKDEARIAQALRTGGGLL
                     WADHDEGLFLGTARFFKPGYVGNIVQNWIPALTGIEAKLVTGGMVVDVGCGHGTSTII
                     MAKAYPRSRFFGFDSHGPSIEAARRSAEEAGVADRVTFDVASAQNFPGRGYDLVTYFD
                     CLHDMGDPEGAARHAHEALDADGSVMLVEPMAGETVEDNLNPVGRLFSAASVLVCTPH
                     AIAEGGRALGTIATDAELRAVFNGAGFTRFHRAVETPTNRVFEARL"
     gene            1062..1439
                     /locus_tag="VNL92_07960"
     CDS             1062..1439
                     /locus_tag="VNL92_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWO94683.1"
                     /translation="MSTHVDLIAEMTQQFRPLLDSSPDGVYLWVDEETKVCNENLARM
                     FGYTIDEWRGTQPFLDNFVAPDDRELYSWNYHRCVAQLAFPVTFRFRGQRKDGSTFAA
                     ETDMIPISWGGHPVAYHFVRRLG"
     gene            1619..2314
                     /locus_tag="VNL92_07965"
     CDS             1619..2314
                     /locus_tag="VNL92_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWO94684.1"
                     /translation="MCNRFTLTLADRVFLAAQLGVPVSDIPEEDYRPRYNIAPTDWHW
                     ILRVKGEDREVLPARWGLVNSWTKEGTRPSRHLNARAETLAKRGVFKDAFERRRCAIP
                     ADGFFEWRGPPQARQPIWYHKRDGGLLLFAGLYESWRPRPDEWERTFTIVTTAPNRLV
                     AEVHDRMPVVLEGAALGDWLFGQTRDAEELLGLLRPAPEELLIATPVSPRVNSVRNDD
                     PACLEPMEQPAVG"
BASE COUNT          425 a          768 c          793 g          422 t
ORIGIN      
        1 attggcgaca agctcggctt gtacaaggcc ctgcacgagc tcgggcccgc gacgcctggc
       61 gaactcgcgg ctcgcactca cacgagcgag agatacctcc ggcactggct gctcaaccaa
      121 gcagcctccg gatacatcac gtactccgcc gcgacaggga aatactcgct ctctccggag
      181 caagcaatgg tcttcgccga cgacgacagc ccggcggcga tggcgggcgg cttcggactc
      241 atgacgtcgc tcgtgaagga tgaggccagg attgcgcaag cgttgcgaac gggcggcggg
      301 cttctctggg ctgaccacga tgagggccta ttcctaggca cggcacgctt cttcaagccg
      361 ggctacgtag gcaacatcgt ccagaactgg atcccggccc ttacggggat agaggccaag
      421 ctcgtgaccg gtggcatggt tgtcgacgtt ggctgtggcc atggcacttc gaccatcatc
      481 atggcgaagg cctacccgag gtcgcgcttc ttcggcttcg acagtcacgg cccctccatc
      541 gaggcggccc gacgatctgc tgaggaagcc ggcgttgctg accgggtgac ctttgatgtc
      601 gcgtcggccc agaacttccc agggcggggc tacgacctcg tgacgtactt cgattgccta
      661 cacgatatgg gcgacccgga aggcgcggct cgtcatgcac atgaggctct cgacgccgat
      721 ggatcggtga tgctcgttga gcccatggcc ggtgagacag tcgaagacaa cctgaatccg
      781 gtgggccgcc tgttctccgc cgcctcggtt ctcgtctgca cacctcatgc cattgcggaa
      841 ggtgggcgag cacttggcac catcgccacc gacgccgagc tgcgggccgt cttcaatgga
      901 gctggcttca ctcggttcca ccgtgccgtc gagacgccga cgaaccgggt attcgaggcg
      961 aggctgtaat cggagccgtg agctcacgtg aggccctgga gtggcagaag gacagcggag
     1021 ggttggccgt taggcgccaa caggaaagga gcaaacagga tatgagcacg catgtcgacc
     1081 tcatagcgga gatgacccaa cagttccgtc ccctgctcga tagcagtccc gacggtgtct
     1141 acctctgggt ggatgaggag acgaaggtct gcaacgagaa tcttgcccga atgttcggct
     1201 acacgatcga cgagtggcgc ggaacgcagc cgttcctgga caacttcgtc gcgcccgacg
     1261 accgggagct gtattcctgg aactaccacc gctgcgtggc gcaactggcc ttcccggtga
     1321 ccttccgatt ccggggacag cgcaaggacg gctcgacgtt tgcggcggag acggacatga
     1381 tcccgatctc ttggggcggt caccctgtcg cctaccactt cgtccgtcgc ctcggctagg
     1441 catcccgcga cgcccaatcg gcccgctatc gttcccggcc cctcgcccgc tgacctacgt
     1501 aaaccagcgg gcgaggggcg tgcgtgcttt tgatggcgac gactcccgcc aatggcgcgt
     1561 ttcgatgctg gattggccgg gcaggacgcc gcacggcgcc gactggtaga ctcggattat
     1621 gtgcaatcga ttcacgctca cgctcgcgga ccgcgtcttc ctcgccgctc agctcggcgt
     1681 gcccgtctcg gatattccgg aggaggacta ccggccgcgc tacaacatcg cgccgacgga
     1741 ctggcattgg atcctgcgcg tcaagggcga ggaccgcgag gttctcccgg cgcgctgggg
     1801 tcttgtgaac agctggacga aggagggcac acgccccagc cgtcacctca atgcgcgagc
     1861 ggagacgctg gcgaaacggg gcgtcttcaa ggatgcgttc gagcgaagac gctgcgccat
     1921 ccctgccgac ggctttttcg agtggcgcgg tccgccgcag gcccgccaac cgatctggta
     1981 tcacaagcgc gacggaggtt tgctgctctt cgcagggctc tacgagtcgt ggagacctcg
     2041 ccccgacgag tgggagcgaa ccttcaccat tgtcacgacg gcccccaatc gcctcgtggc
     2101 cgaggtccac gacaggatgc ccgtggttct ggaaggcgcg gcgctgggcg attggctctt
     2161 cgggcagacg cgtgacgccg aggagctgct cggccttctg cggccggcgc ccgaggaact
     2221 gctgatcgcg acgccggtat cgccgcgggt gaactcggtg cgaaacgacg atccggcttg
     2281 cctcgagccc atggaacagc cggcggtggg ctgaccctgc tgcatgcggg aggaggaacg
     2341 tatggcggaa cgacacgcgg atcggcggga gttccttcag tcgttggagg gcctcgaatt
     2401 ccccgcgc
//
LOCUS       DATMBB010000374         6323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_133800, whole genome shotgun sequence.
ACCESSION   DATMBB010000374 DATMBB010000000
VERSION     DATMBB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133800"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="VNL92_07970"
     CDS             <1..2043
                     /locus_tag="VNL92_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWO94685.1"
                     /translation="ELLLSRRLPAQPPRDFVQTVYNQTEGNPFFIEEVLAHLLETGAL
                     ERHLANAHSSTDALGIPEGIREVVGRRLNRLSNECNRVLTHASVFGREFQFDVLRRMA
                     GLDDETLLGALDEALDSELVIERPGNLLATYAFSHALVRGTLYDELSLPRKQRLHLLA
                     AQAIEDAHARDLERHISALATHYRLAGAAADLDKAIEYSLRAGEAARRSFAYEEARAH
                     WESALELMEERRADQPQQAQLLERLGDLMYISNAQPLIGIDYLERSLALYEQLGDMDR
                     AARLHSRLGRDLSSYARDIDRALEHYRAAEPVLAAQGDSAALAYLYNGIATAAIWSMR
                     TAEGLEAASRAIALAERLGSESARVVGESHLGYHQWASGRISAGTRRIEEAWSMADRL
                     NRPTLAYVAAWERGLCATYSLAPLEGRDWSRRELDQPRIAQAEAQRQGLLMQLATNLI
                     FAGDKAAADEVWTQVSRGSVERVSGQALFAYWMGDWDAARESWGIARGRAAATGDKFV
                     LCGDAFWLSQLLGVTGDFEEAERVLAEALDIALHGEHRALEVALRSQLGLLHAHTGRI
                     DSARAHVQRCEELTSDDDWRGLAARTALACAVLHRVAGERAMAEERFAEALLIGEREQ
                     LALHCAETLHEWGRALVELGDPAGATLKFSSAIDIYRTRGAGEPWIARVERDKAAL"
     gene            2080..3258
                     /locus_tag="VNL92_07975"
     CDS             2080..3258
                     /locus_tag="VNL92_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO94686.1"
                     /translation="MEFRFTEAEERWREEIRSFLAAELPRESRIGADWTQSQEAWDRS
                     LEFVRRVAERGWLTLAWPKQYGGQERSHMEQLIFNEEMAYHRAPMARAGIGTGLVAPT
                     LMIHGNDAQRGRHLPAIARAEEMWCQGFSEPNAGSDLAGLETRAVHDGDDFVVNGQKI
                     WTSAAHHCDWCLLGVRTDSEAPKHRGISLLMVDMKSPGISVQPIVDMRDSHYFNQVFF
                     ENVRVPRENLIGEENRGWYAMTTTLDFERSGIGMTAGMRRTLEDWLAFIREHGHRHID
                     TAVWEAVRRRIADIVIEIEVGRTISYRVVSLQARGLVPNYEASMAKLYGSELSQRLAA
                     LGMHLLGLYGELRPGSEHALMGGRVLPQFLESAANTIRGGTSEVQRNIIATRGLGLPR
                     "
     gene            3297..4265
                     /locus_tag="VNL92_07980"
     CDS             3297..4265
                     /locus_tag="VNL92_07980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO94687.1"
                     /translation="MVVEAKQRILCPLGCDVAPEAAPYPVARYRADRCIERGVSLQTR
                     RPGWVDILRCPACGLEWLDAQIAWQTSPEDAHLGLALQYGADPQAERRRAQRFLATVH
                     EFRRPPGRLFDIGCGMGELLRQARAAGWDVAGNDIVAGAMERLRADGFEVYTGDLTDL
                     HLPVASFDVVTAFCVLPHLADPVEEMRSVARMLAPSGFFVAEAPADGPYRRSARALAR
                     LGIDWGVRNVYYPGHRFAFGEPSLRGLFARAGLELVAIRPFRMSAGTSARRFSLRGGV
                     LGRLGAAGVTLADLATRASRLHNHLIAIARKPAHGSPEYTEDAQRV"
     gene            4384..5532
                     /locus_tag="VNL92_07985"
     CDS             4384..5532
                     /locus_tag="VNL92_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWO94688.1"
                     /translation="MTSETRTDTKIYLGLEDIPVVASSICSIDGVNGRLLYRGYDIAD
                     LASSATFEEVVHLLWFGELPNARQLESLRGDLRREQQLPDAIIDLIRTLPRDGNSMSA
                     LRTLVSALALYAPESDVHVHDEQVREAIRLTVRMGTIVAAWGRHRAGEDPLQPDPDLG
                     LAANFLAMLFGRRPDPAEEHAFDVALILHADHELNASTFAARVTAATLSDMYSAVTSA
                     IGALKGPLHGGANEQVMRMLETIRSPEEADEYVRDRLARHERLMGFGHRVYRVEDPRA
                     TQLRRLSKELGELKGDTRWYDTSIAIEQAVRRERGLNPNVDFYSASLYHTLGIPTELF
                     TPIFAVSRISGWTAHILEQYEHNRLIRPRAEYTGSEGRQYVSVEQRSA"
     gene            5593..>6323
                     /locus_tag="VNL92_07990"
     CDS             5593..>6323
                     /locus_tag="VNL92_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94689.1"
                     /translation="MRDVAYVANWLSEVGALGGVARFLDASLPQPPDLTALSGHAFQL
                     VVREGPEGLFPIAAPAGPSYARAVAQYSVLGLRGRIYEVRPEDRASRDEALRAVRRSI
                     DRKRPVIAFGLHIPEFGIIRGYDDDRAQLIVSTLTTEQHGPALPLALWPAPNTKEAIP
                     IVVVDRERRIRTEVAEQEALAFAVREYEAKTGAGELAVGAAGYECWISALGSERAALH
                     GRGHAHAVQKLLAARREAAQFLAGLA"
BASE COUNT         1057 a         2160 c         2095 g         1011 t
ORIGIN      
        1 gagctgctcc tcagccggcg ccttcccgct cagccaccac gcgacttcgt gcagacggtg
       61 tataaccaga cggaaggcaa ccccttcttc atcgaggagg tgctcgctca tctcctcgag
      121 accggcgcac tcgaacggca cctcgccaac gcccacagct cgaccgatgc gctcggcatc
      181 cccgaaggca tccgcgaggt cgtgggacgt cgcctcaacc gactctcgaa cgagtgcaac
      241 cgcgtcctca cgcacgcctc cgtctttggt cgcgagttcc agttcgacgt actgcgccgg
      301 atggcgggtc tcgacgacga gacgctcttg ggcgcattgg acgaggcgct cgactcggag
      361 ctcgtgatcg agcggccggg gaacctcctg gcgacctacg ccttcagcca tgccctcgtt
      421 cgaggcacgc tctacgacga gctgagccta cctcgcaagc agcgtctgca tttgctggcc
      481 gcgcaggcca tcgaggacgc tcacgctcgc gacctggagc ggcacatctc ggccctcgcg
      541 acgcactacc ggctcgcggg cgccgcggcc gacctcgaca aggcgatcga atactcgttg
      601 cgggccggcg aggccgcgcg ccgttccttc gcgtacgagg aagcccgggc gcactgggaa
      661 agcgccctcg agctcatgga ggagcggaga gcggaccagc cacaacaggc gcagttgctg
      721 gagcgcctcg gcgacctcat gtacatcagc aatgcccagc ccttgattgg gatcgactat
      781 ctggagcgct cgctcgccct ctacgagcag ttgggggaca tggaccgtgc cgcacggctc
      841 cactcgaggc tcgggagaga cctctcgtct tatgcgcgcg acatcgatcg cgccttggag
      901 cactatcgag cggccgagcc tgtgctcgcc gcgcagggcg atagcgctgc cctcgcctac
      961 ctgtacaacg gcatcgccac ggccgcgatc tggagcatgc ggaccgccga ggggctcgag
     1021 gctgcgtctc gggccatcgc tctggcggag cggctcggct ccgaatccgc ccgcgtcgtg
     1081 ggcgaatcgc acctcggcta ccaccaatgg gcgagcggca ggatcagcgc gggcactcgg
     1141 cggatcgaag aagcctggtc gatggccgat cggctcaacc ggccaaccct cgcctatgtc
     1201 gcagcctggg agcgcgggct gtgcgccacg tactcgctgg cgccgctcga ggggcgcgac
     1261 tggagccggc gcgagctgga ccaaccgagg attgcccagg cggaggcgca acgacaaggc
     1321 ctgctcatgc agctggcgac gaacttgatc ttcgcaggcg ataaggccgc ggccgacgag
     1381 gtatggaccc aggtgtctcg tggcagcgtc gagcgcgtca gcggccaggc actcttcgca
     1441 tactggatgg gcgactggga cgccgcccgt gagagttggg ggatcgctcg cggccgggct
     1501 gccgcaacag gtgacaagtt tgtcctctgc ggcgacgcgt tttggctgtc tcagctcctg
     1561 ggagtcaccg gcgacttcga ggaggcggag cgcgtgctcg ccgaggcgct cgacatcgcc
     1621 ctgcacgggg agcaccgcgc cctcgaggtg gcgctacgct cgcagctcgg gctcctccat
     1681 gcgcatactg gccgcatcga ttccgcgaga gcccacgtgc aacgctgcga ggagctcacc
     1741 agcgacgacg actggcgtgg cttggcggca cgcacggcgc tcgcctgcgc cgttctccat
     1801 cgcgtcgccg gcgagcgggc gatggccgaa gaacgattcg ccgaggccct cctcatcggg
     1861 gagcgagaac agctcgccct gcactgcgcc gagacattgc acgagtgggg ccgggccctc
     1921 gtcgagttgg gcgacccggc aggagcgacg ctcaagttct cgagcgcgat cgacatctac
     1981 cgaacccgcg gcgccggcga gccgtggata gcccgcgtcg aacgcgacaa ggcagccctg
     2041 taggatcgcg tccacgggcc gcagaccgag ggacgaacca tggaatttcg attcaccgag
     2101 gccgaggaac gctggcgcga ggagatacgt tcgttcctcg ccgctgagct gccgcgcgaa
     2161 tcccgcatcg gggcggactg gactcagtcg caggaggcat gggaccgctc cctcgagttc
     2221 gtccgcaggg tggcggagcg aggctggctc acgctcgcgt ggccgaaaca gtacggtggc
     2281 caagaacgct cgcacatgga gcagctcatc ttcaacgagg agatggccta ccaccgcgcc
     2341 ccgatggcac gtgccggaat cggcaccggg ctcgtggccc caacactcat gatccacggc
     2401 aacgatgctc agcgcggccg ccacctcccc gccatcgcgc gcgccgagga gatgtggtgc
     2461 cagggattca gcgagccgaa cgccggctcc gacctggcag gactggagac gcgcgccgtc
     2521 cacgatggcg acgacttcgt cgtcaacgga cagaagatct ggacttctgc cgcccaccac
     2581 tgcgactggt gcctcctcgg cgtccgcacc gactccgagg cgccgaagca ccgcggcatc
     2641 tcgcttctca tggtcgacat gaagtcaccg ggcatctccg tccagccgat cgtcgacatg
     2701 cgggacagcc actacttcaa tcaggtgttc ttcgagaacg tgcgcgtgcc tcgagagaac
     2761 cttatcggcg aggagaaccg gggctggtac gccatgacga ccacgctcga tttcgagcgc
     2821 tcgggcatcg gcatgacggc aggcatgcga cgcaccctcg aggactggct cgccttcatc
     2881 cgggagcatg gtcatcgcca catcgatacg gcggtgtggg aggcggtccg aaggcgcatc
     2941 gcggatatcg tcatcgagat cgaggtcggc aggaccatct cgtaccgcgt cgtttcgctg
     3001 caggcccgcg gcctggtacc gaactacgag gcgtcgatgg ccaagctcta cggcagcgaa
     3061 ctctcgcagc ggctcgcggc cctgggcatg cacctgctcg ggctctacgg ggagcttcgt
     3121 cccggttcgg agcatgccct catgggcgga cgcgtgcttc cccaattcct cgaatcggcg
     3181 gcaaacacga ttcgaggcgg cacgagcgag gttcagcgca atatcatcgc caccaggggc
     3241 ctcggcctgc cgcgctagcc ggccccagac ctccgttcgg ggcgtatact cggatcgtgg
     3301 tagttgaggc gaagcagcgc atcctatgcc ccctgggctg cgacgtggcg cccgaggcgg
     3361 ccccctaccc tgtcgctcga taccgtgcgg accgatgcat cgagcgaggc gtctcgctcc
     3421 agacgcgccg gccgggctgg gtcgacattc tgcgttgccc tgcctgcggc ttggaatggc
     3481 tggacgccca gatagcgtgg cagacatcgc ccgaggatgc ccacctcggc ctggcgcttc
     3541 agtacggcgc cgatccacaa gcggaacggc ggcgagcaca gcgtttcctc gcgaccgtcc
     3601 acgagttcag acgaccgccg ggccggctct tcgatatcgg gtgcggcatg ggcgagctcc
     3661 tgcgccaggc gcgagcggcg ggctgggacg tggctgggaa tgacatcgtc gccggggcca
     3721 tggaacgcct acgagcggat ggattcgagg tgtacacggg tgacctcacg gacctgcatc
     3781 tgcccgttgc gtcgttcgac gtggtcacgg cgttttgcgt gctgccccac ctggccgacc
     3841 ccgtcgagga aatgcgttcc gtcgcgcgca tgctcgcacc cagtggcttc ttcgtcgcgg
     3901 aagcaccggc agatggacca tatcgccgca gcgcccgagc cctggcccgc ctcgggatcg
     3961 attggggcgt ccgcaacgta tattatccgg gccaccgctt cgcctttggc gaaccctcgc
     4021 ttcgcgggct cttcgcgcgg gcgggattgg agttggtagc tattcgcccg tttcgaatgt
     4081 cggccggaac gagcgcacga cgcttctcgc tccggggcgg cgttctgggc cgcctcggag
     4141 cggccggtgt cacccttgcc gacctcgcaa cgcgagccag caggctgcac aatcacctga
     4201 ttgccatcgc caggaagccc gcgcatgggt caccggaata tactgaagat gcgcagcgcg
     4261 tctgacccgt tgaccgccag gcagcgctgc gagatagttc cagcggaggc agtcacaggt
     4321 tagattcgta cgtcgatcgc ccccgtgaga tacggtattc gcccacaatg gaggggcata
     4381 gaagtgacga gcgagacaag aactgacacg aagatctacc ttggcctcga agatatcccc
     4441 gtcgtggcat cgtccatctg ctccatagac ggggtcaacg gccggctact gtaccgtggc
     4501 tacgatatcg ccgacctggc gagcagcgcg accttcgagg aagtcgtgca tctcctctgg
     4561 ttcggcgagc taccaaacgc ccggcagctg gaaagcctgc gaggcgatct ccggcgcgag
     4621 cagcaactgc ccgatgccat catcgacctg atccggaccc tccccaggga cggcaatagc
     4681 atgtctgccc tgcgcacgct ggtttcggcg ctggccctgt acgcgccgga aagcgacgtt
     4741 cacgtgcacg acgagcaggt gcgggaggct attcgcctca ccgtccggat gggcaccatc
     4801 gtcgccgcct gggggagaca ccgggccggc gaagatcccc ttcagcccga ccccgatctt
     4861 ggccttgcgg ccaacttcct cgccatgctc ttcggccggc ggccggaccc tgcagaggag
     4921 cacgccttcg atgtggcgct catcctccat gccgaccacg agctgaatgc ctcgacgttc
     4981 gcggccaggg tcacagcagc cacgctgtcc gacatgtact ccgccgtcac atccgcaatc
     5041 ggcgccctca agggaccgct gcacggcggc gccaacgagc aggtgatgcg catgctcgag
     5101 accatccgct cgcccgagga agcggacgag tacgtgcgcg ataggctggc gcgccacgag
     5161 cggctcatgg gcttcggcca tcgcgtgtat cgcgtggagg acccgcgggc aacgcaactg
     5221 cgaaggctct cgaaggagct gggcgaactg aagggcgaca cgcgttggta cgatacctcg
     5281 atcgccatcg agcaggcggt gcggcgcgag cgcggcctga acccgaacgt cgatttctac
     5341 tcggcctcgc tctaccacac gctcggcatc ccgacggagc tgttcacgcc catctttgcg
     5401 gtcagccgca tctcggggtg gaccgcgcac atcctggagc agtacgagca caaccggctc
     5461 atccgcccgc gcgccgagta cacgggctcc gaagggcgcc agtacgtatc ggtggagcag
     5521 cgttcggcct aagccgcttc gttgacggcc tgacttcgcc ttcctatcct cgatcctgtg
     5581 gctcggaagg agttgcgcga cgtcgcctac gtggcgaact ggctgtcgga ggtcggggcg
     5641 ctcggcggcg tggcgcgctt cctcgatgcc agcctgccgc agccgcccga cttgacggca
     5701 ctcagtggcc acgcgttcca gctggtcgtc cgcgaggggc ccgaaggact cttccccatc
     5761 gctgcaccgg cgggcccctc ctatgcccgc gctgtcgccc agtactcggt cctcgggctg
     5821 cgcggtcgga tctacgaggt gcgaccggag gatcgggcta gccgagacga ggcgctccgt
     5881 gcggtccggc ggtccatcga ccgcaagcgg cccgtgatcg cgttcggcct gcacatcccc
     5941 gagttcggca tcatccgcgg ctacgacgac gaccgggccc agctcatcgt aagcacgctc
     6001 acgacggagc agcatggccc cgcgttgccg ctcgcgctct ggccggcgcc gaacaccaag
     6061 gaagcgattc ccatcgttgt cgtggaccgc gaacggcgaa tccgcaccga ggtggcggag
     6121 caggaggcac tcgcgttcgc cgttcgcgag tacgaggcga agactggtgc cggagagctt
     6181 gcggtaggcg cggccggtta cgaatgctgg atttcggcgc tcggaagcga gcgtgcggcg
     6241 ctgcacggtc gcggtcacgc ccacgcggtt cagaagctgc tcgccgcccg aagggaggct
     6301 gcccagttcc tggcaggcct cgc
//
LOCUS       DATMBB010000375         3375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_337548, whole genome shotgun sequence.
ACCESSION   DATMBB010000375 DATMBB010000000
VERSION     DATMBB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337548"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2899
                     /gene="carB"
                     /locus_tag="VNL92_07995"
     CDS             <1..2899
                     /gene="carB"
                     /locus_tag="VNL92_07995"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWO94690.1"
                     /translation="DAAIAIMRAVGVDTGGSNIQFAVHPRDGRMVAIEMNPRVSRSSA
                     LASKATGFPIAKIAAKLAIGYTLDELRNDITRVTSACFEPAIDYVVVKIPRFAFEKFP
                     EADETLTTSMKSVGEAMAIGRSFKEALQKCIRSMEIKRPGYGLHPKDKWLDARQDAAL
                     ADSGDDVWIDTFGNEAGASGPVFGGATLGEPPADTREAAVRAWPIPEDVLTAKIGTPC
                     QGRLYYIRYALKQGWSVERIHELSKIDPWFLDQLAELVEFEQRLQEVRVENGQALRLT
                     PDLIEVFQQARAWGYSEAQLRCIWRIDAETMRRIRAQIDRPKFKLVDTCAAEFEAFTP
                     YYYSTHERPLAQIVRSPAAVAELRSHSASRGRVPLGEAVRNLGVPSPYRYRVLDDDHV
                     LVETHDDELRLTDKPKVVILGGGPNRIGQGIEFDYCCVHAAQAARAMGYETVMINSNP
                     ETVSTDYDISDLLFFEPLTHEDVMTICERLNDRPFSQRGGLVRGVVVQFGGQTPLNLA
                     QGLHDAGVPILGTSPASIHAAEDRERFQQVLRDLGLRQPPNGVAYSRAEAVEVAEKLG
                     YPVLVRPSYVLGGRGMQVCNNVGDLVRYIDGALAATDRELNISRQHPILIEKFLVDAI
                     EVDVDAIADFGYPGRDLSEGRCDICGIMEHIEEAGIHSGDSCCVLPPYSLSPAIVDEI
                     KYQVKLLARKLGVCGLMNVQFAIQGRVVYLIEVNPRASRTIPFVSKATGVPWARIATR
                     VMLGQSLDEALAGVSLAEVRSHRHVAVKVPVFPFKKLRTVDFALGPEMRSTGEVMGID
                     ESYPMAFAKGLLAAGMRLPVSGRVLVSVCDADKPRVISIARELHEMGFEIYSTIKTKQ
                     VLAEHGIPAQLVSKHDGHGAPFLLDKIRNRELDLLINTPIRTGSATEEGRWRVASIQL
                     GIPLITTIEGAQAAVGAIRALRSGTADVKALQDYLSSNPPPPRAVVHGGVE"
     gene            2896..>3375
                     /locus_tag="VNL92_08000"
     CDS             2896..>3375
                     /locus_tag="VNL92_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HWO94691.1"
                     /translation="MTVAPARQAKLALEDGSIFTGLAVGAIGTRVGEVVFNTAMTGYQ
                     EVFTDPSYCGQIVTMTFPLQGNYGVNEEDFEAARPHLSGVVLREVPRRPSNYRATRSL
                     PEFLEKQNIVGILGVDTRAITRRVRIQGALRGVLSSEILDDLELVRRARAAAAMEGAN
                     "
BASE COUNT          582 a         1249 c         1016 g          528 t
ORIGIN      
        1 cgatgccgcg atcgccatca tgcgcgcggt gggcgtcgac accggcggct ccaacatcca
       61 gttcgccgtc cacccgcgcg acggccgcat ggtcgcgatc gagatgaatc cgcgcgtgtc
      121 gcgctccagc gccctggcct ccaaggcgac gggcttcccg atcgcgaaga tcgcggccaa
      181 gctcgccatc ggttacacgc tcgacgagtt gcgcaacgac atcacgcgcg tcacctccgc
      241 ctgcttcgag cccgcgatcg actacgtggt ggtcaagatc ccgcgctttg cattcgagaa
      301 gtttcccgaa gccgacgaga cgctcaccac ttcgatgaag tccgtcggcg aggccatggc
      361 catcggccgc tcgttcaagg aagcccttca gaagtgcatc cgctcgatgg agatcaagcg
      421 acccggttac gggctgcacc cgaaggacaa atggctcgac gccagacagg acgccgccct
      481 ggccgactca ggcgacgatg tctggatcga caccttcggc aacgaagccg gtgcgagcgg
      541 ccccgttttc ggaggcgcaa ccctcggcga gccgccggcc gacacccgcg aggccgctgt
      601 acgagcctgg ccgattccgg aggacgtgct caccgccaag atcggcacac cctgccaggg
      661 ccggctctat tacatccgct atgcgctcaa gcagggttgg agcgtcgagc gcattcatga
      721 gctttcgaag atcgacccgt ggtttctcga ccagctcgcg gaactggtcg agttcgagca
      781 acgactccag gaagtccgcg tcgagaacgg gcaagcgctg cgactcacgc cggacctcat
      841 cgaagtcttt caacaggccc gcgcctgggg ctacagcgaa gcccagcttc gctgcatctg
      901 gcgcatcgac gccgaaacca tgcgccgcat ccgcgcccag atcgatcgtc ccaaattcaa
      961 gctcgtggac acctgcgcgg cggagttcga ggccttcact ccttactact acagcacgca
     1021 cgagcgcccc ctcgcccaga tcgtccgcag ccccgccgcc gtcgctgagt tgcgcagcca
     1081 cagcgcgtcg cgcggccgcg tcccgctcgg cgaggccgtt cgcaacctcg gcgttccgtc
     1141 gccctatcgt taccgcgtgc tcgacgacga tcacgtgctg gtcgagaccc acgacgacga
     1201 gctgcgcctc accgacaagc ccaaggtcgt catcctgggc ggcggcccca accgcatcgg
     1261 ccagggcatc gagttcgact actgctgtgt ccacgccgcg caggccgccc gcgcgatggg
     1321 ctacgaaacg gtgatgatca actccaaccc cgagaccgtc agcaccgact acgacatcag
     1381 cgacctgctc ttcttcgaac cgctcaccca cgaagacgtc atgacgatct gcgagcgact
     1441 caacgaccgc ccgttctccc agcgcggcgg cctggtgcgc ggcgtcgtcg tccagttcgg
     1501 gggccagacg ccgctcaatc tcgcccaggg cctgcacgac gcgggcgtgc cgatcctcgg
     1561 aacttccccg gccagcatcc acgccgccga agaccgcgaa cgcttccagc aggtgctgcg
     1621 cgacctcggc ctgcgccagc cgcccaacgg tgtggcatat tcgcgggccg aggcggtcga
     1681 agtcgccgag aagctcggct accccgtgct ggtccgcccg tcttacgtgc tcggcggccg
     1741 cggcatgcag gtctgcaaca acgtcggcga cctggttcgc tacatcgacg gcgcgctcgc
     1801 cgccaccgat cgcgagctga acatcagccg gcagcatccc atcctcatcg aaaagttcct
     1861 cgtggacgcg atcgaggtgg acgtggatgc catcgccgac ttcggctacc ccggccgcga
     1921 cctcagcgaa ggccgctgtg acatctgcgg catcatggag cacatcgagg aagccggcat
     1981 ccacagcggc gacagttgct gcgtgctgcc gccctattcg ctctcccccg ccatcgtgga
     2041 cgagatcaag tatcaggtga agctgctggc gcggaagctg ggcgtctgcg gcctgatgaa
     2101 cgtgcagttc gccatccagg ggcgcgtggt ctacctcatc gaggtgaacc cccgcgcctc
     2161 gcgcaccatt ccattcgtca gcaaggccac gggtgtgccg tgggcccgca tcgccacgcg
     2221 ggtcatgctc ggccagtcgc tggacgaagc cctcgccggc gtcagcctcg ccgaggtccg
     2281 ctcgcaccgc cacgtcgcgg tgaaagtgcc ggtctttccc ttcaagaaac tccgcaccgt
     2341 cgacttcgcc ctcggccccg aaatgcgcag caccggcgaa gtcatgggca tcgacgagag
     2401 ctacccgatg gccttcgcca agggtctgct cgccgcaggc atgcgcctgc ccgtttccgg
     2461 tcgcgtgctg gtaagcgtct gcgacgccga caagccgcgc gtcatctcca tcgcccgcga
     2521 gctgcacgag atgggcttcg agatttactc caccatcaag acaaagcagg tgctcgcgga
     2581 gcatggcatc ccggcccaac tcgtctccaa gcacgacggc cacggcgccc ccttcctgct
     2641 cgacaagatt cgcaaccgcg aactggacct gctcatcaat acgcccatcc gcacgggcag
     2701 cgccacagag gaaggccgct ggcgcgtggc gtcgatccag ctcggcatcc cgcttatcac
     2761 cacgatcgaa ggggcccagg ccgcggtcgg cgccatccgc gccctgcgca gcggcaccgc
     2821 cgacgtaaag gcgcttcagg actatctttc ctcgaacccc ccgccgcccc gcgcggtcgt
     2881 gcatggaggc gtcgaatgac cgtcgcaccc gcccgccagg ccaagctcgc cctcgaagac
     2941 ggctcgatct tcaccggcct cgccgtcggc gccatcggca cgcgcgtcgg agaggtcgtc
     3001 ttcaacaccg ccatgaccgg ctaccaggag gtcttcaccg acccctccta ctgcggccag
     3061 atcgtgacga tgacctttcc gctccagggc aactatggcg tgaacgagga ggacttcgaa
     3121 gccgcccggc cgcatctctc cggcgtcgtg ttgcgcgagg ttccgcggcg gccgagcaac
     3181 taccgcgcga cccgttcgct gcccgagttc ctcgagaagc agaacatcgt cggcatcctc
     3241 ggcgtggaca cgcgcgccat cacccgccgc gtccgcatcc agggcgcgct ccgcggcgtg
     3301 ctctcttccg agattctcga cgacctggag ctggtccgcc gcgcccgcgc ggccgccgcg
     3361 atggagggcg cgaat
//
LOCUS       DATMBB010000376         4562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_577650, whole genome shotgun sequence.
ACCESSION   DATMBB010000376 DATMBB010000000
VERSION     DATMBB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577650"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VNL92_08005"
     CDS             complement(<1..407)
                     /locus_tag="VNL92_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWO94692.1"
                     /translation="MERQRPPDENGQGDEVAGSQAFASVADLETRPRSELVDIARGLG
                     ISGYATMSKQDLVFRLIQAHEQQQAQQEPDQLFSGGVLEIVDDGYGFLRGESLLPSLN
                     DVYVSQSQIRRFGLRTGDFVRGQIRTPKDGEKY"
     gene            complement(532..1206)
                     /gene="fsa"
                     /locus_tag="VNL92_08010"
     CDS             complement(532..1206)
                     /gene="fsa"
                     /locus_tag="VNL92_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HWO94693.1"
                     /translation="MRLFLDTANIHEIRQGVELGVISGATTNPSLIAKERGVPYRERV
                     IEIADLVQGPVSAECLARTADELVREAREIASWHPNVVVKVPMDAEGLKAIRTLSAEG
                     ILINTTLIFSSNQALLAATAGATYVSPFVGRLDDIGEDGIALVREIVEIFDRYHLPTQ
                     VLAASLRHPRHVTEAARAGAHIATMPFSVLNQMLKHPLTDIGIERFIQDYADAQQYLK
                     ASVETR"
     gene            complement(1348..1827)
                     /locus_tag="VNL92_08015"
     CDS             complement(1348..1827)
                     /locus_tag="VNL92_08015"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HWO94694.1"
                     /translation="MSADSVLRLLHILSLFLMVGGLLAVMLELVRAWTIDDVGRQIQL
                     FEEADVAKVTLLVPGAIFTGITGFVWAIQQEYDFVEDGWLFALVAVYLFSVVICLPLL
                     NLGLRRVQLLAMQARKRGDERTPELEDALADNVPLVFGSIMLLTVVIMAWLPIFKPF"
     gene            complement(1824..3491)
                     /locus_tag="VNL92_08020"
     CDS             complement(1824..3491)
                     /locus_tag="VNL92_08020"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWO94695.1"
                     /translation="MPKYIFVTGGVVSSVGKGITTAALGRILKSRSLSVSIQKLDPYL
                     NVDPGTMSPYQHGEVFVTMDGAETDLDLGHYERFIDAELTRLSSVTAGQIHQAVIARE
                     RRGDYLGRTIQTIPHVTDEIKQRIRLLGKTSGADVLIIEVGGTVGDIEGQPFLEAIRQ
                     MRHEEAREDTLSIHVTFLPYIAATQELKTKPTQHSVRDLRSIGIQPDVIVARSDHPVP
                     ADIRGKVALFCDVEPRAVVPLETLSSIYEVPLALEAESVGNYIVERMHLPAAAPELSS
                     WAEMVQRLRQPQDYVEIAIAGKYVELPDAYISVKEALTHAAVQNGVGLRVRWVHAEAL
                     EGPAGVDLLRDVDGIVVPGGFGERGIEGKILAADYAWDEEIPYLGLCLGMQVMVVAAA
                     RRRFQSREPNSTEFQPGTAYPVISLLSEQEGIEDKGGTMRLGAYPCRLMPGSKAYEAY
                     GREEIVERHRHRYEFNNAFAADLAEVGLMASGRSPDGTLVEISEVAGHPWMVGSQFHP
                     EFGSRPNAPHPLFSGFVRAALERARVRRRRTTAAEALETSGSGVAGS"
     gene            complement(3566..4123)
                     /locus_tag="VNL92_08025"
     CDS             complement(3566..4123)
                     /locus_tag="VNL92_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO94696.1"
                     /translation="MEHAGLLERTYEDLPISPRLEEVATQINKGEVPNAGRFCGNCYT
                     PQRADRELCPYCGTSTTERSPVDLIPPQVLAIVKAKRRRESWIVHSFAYTGLVLGTAI
                     AIAMLIVLPGWWKLTALVVLLFGTRGLAAILGGWLGDVLGYQSARRLVQELWSQHLAE
                     RDAERGSQEARQGHRPIDNNRRINS"
     gene            complement(4143..>4562)
                     /locus_tag="VNL92_08030"
     CDS             complement(4143..>4562)
                     /locus_tag="VNL92_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWO94697.1"
                     /translation="EIDSESVRSNVMSGITRRFGYLGWGSLVILLVTGIGNTFQRQSD
                     LEFFGRDVFDFDYRYAWLLTAKLVLVAIVVVLTAWHSFVLGPRMLDEQRDGAPAEDQA
                     SLRRLSIIVSAANLVILLAIVYLVTLMQDPDFSSEEV"
BASE COUNT          828 a         1420 c         1462 g          852 t
ORIGIN      
        1 tagtacttct ctccgtcctt gggcgtccgg atctggcctc gcacgaagtc gcccgtgcga
       61 agcccgaagc gtcggatctg cgactgcgaa acgtagacat cgttgagcga cggtagcaag
      121 ctttcgccgc ggaggaagcc atagccatcg tccacgattt cgagcacgcc gccgctgaag
      181 agctggtcgg gctcctgttg cgcctgttgc tgttcgtgtg cctggatcag ccggaagaca
      241 aggtcctgtt tggacatcgt cgcgtagcct gagatgccga gcccgcgcgc aatatcgacg
      301 agctcggagc gcggacgcgt ctcgaggtcg gcgacgctgg cgaaggcctg cgatccggcg
      361 acttcgtcgc cctggccgtt ctcgtcgggt gggcgctgtc gctccacgaa gggtccgtct
      421 cgacggggcc ttcgtgagcg agagcgggct gcatcggatg gggaataggg ctgtcggccg
      481 cgggattcgc ctaccattcg cctggactgt gctccttgcg ggtctctggg tctaccttgt
      541 ctcaacgcta gccttcaagt actgctgagc atcggcgtag tcttggataa agcgctcgat
      601 gccgatgtcg gtgaggggat gcttgagcat ctggttgagc accgagaagg gcatcgtcgc
      661 gatgtgggcg ccggcgcgag ccgcctcggt cacgtgccga gggtgacgaa ggctcgctgc
      721 gagcacctga gtcgggaggt ggtaccgatc gaagatctcg acgatctcgc gtacgagggc
      781 gatgccatcc tcgccgatat cgtcgagcct cccgacgaac ggactgacgt aggtggctcc
      841 ggccgtggcc gcgaggagtg cctgattgct gctgaagatg agcgtggtgt tgatgaggat
      901 tccttccgcg gaaagggtgc ggatcgcctt caaaccttcc gcgtccatcg gcaccttcac
      961 gacgacgttc gggtgccacg aagcgatttc acgcgcttct cgaacgagct cgtcggcagt
     1021 tcgggccagg cactccgcgg agacgggccc ctggaccagg tcggcgatct cgataacgcg
     1081 ctcccgatac ggaacgcccc gctccttcgc aatgagggaa ggattcgtcg tggcgcccga
     1141 gatcacgccc aactccaccc cctgccggat ttcgtggatg tttgcggtat cgaggaatag
     1201 acgcataggc ttcccccgtt ctcttccgcg cttcctgctc cgctcgactt gggcccgttg
     1261 acgttgtcga gagcggtacc cttctggcgg tggattctct ggtgtgacgg attcctcgcc
     1321 ctatcttcgg acggacccca aatcgtgcta gaacggtttg aagataggca gccacgccat
     1381 aatgacgacg gtcaggagca tgatactgcc gaacacgagc gggacattgt ccgcaagcgc
     1441 gtcctccaac tcgggggtgc gctcgtcgcc cctcttacgg gcctgcatgg cgagcaactg
     1501 aacgcgcctc agacccaggt tgagcaacgg caggcagatg acgacgctga agaggtagac
     1561 ggcaacaaga gcgaagagcc agccgtcctc tacaaaatca tactcctgtt gtatcgccca
     1621 gacaaacccc gtgattcccg taaagatggc cccggggacc agtagggtta ccttcgcgac
     1681 atccgcctcc tcgaacaact ggatctgccg tccgacgtca tcgatggtcc aggcgcggac
     1741 gagttcgagc attacggcaa gtagcccccc aaccatgagg aacaacgaga ggatatggag
     1801 tagccggaga acgctatcgg cgctcacgaa ccggcgacgc cgctcccact ggtctcaagc
     1861 gcttccgctg ccgtcgttct gcggcggcga actctggcac gctccagtgc tgcgcgcacg
     1921 aagccactga acagaggatg gggcgcgttt ggacgcgatc caaactctgg gtggaactgc
     1981 gagcccacca tccacggatg accggcgact tcgcttatct ccacgagggt gccatccggc
     2041 gagcggccgc tcgccatgag ccccacctcg gcgaggtcgg cagcaaaggc gttgttgaac
     2101 tcgtatctgt gccggtgacg ctcgacgatc tcctcgcgcc cgtacgcctc gtaggctttg
     2161 ctgcccggca tgagacgaca tggatacgcg ccgagccgca tcgtcccgcc cttgtcctcg
     2221 atgccctcct gctcagaaag caggctgatc accggatagg cggtgccggg ctggaactcg
     2281 gtggagttgg gttcccgcga ttggaagcgc cgccgagcgg ccgccaccac catcacctgc
     2341 atgccaaggc aaagcccgag gtacgggatc tcttcatccc aggcgtagtc ggctgccaga
     2401 atcttccctt cgatgccgcg ctccccgaat cctccgggaa cgacaatgcc gtcgacatcg
     2461 cggaggaggt cgactccggc ggggccctcg agggcctccg cgtggaccca gcggaccctg
     2521 agaccgacgc cgttctgcac agcggcgtgc gtcagggcct ccttcacgga gatgtaggca
     2581 tcgggtagct cgacgtattt gccggcgata gcgatttcca catagtcttg cggctgcctc
     2641 agccgctgga ccatctcggc ccacgagctc aactcggggg cagccgcggg aagatgcatc
     2701 cgctcgacga tgtagttgcc gacgctctct gcctcgagcg cgagcggcac ttcgtagatg
     2761 ctgctcaacg tctcgagcgg aaccacggcc cgcggctcga cgtcgcagaa gagcgccacc
     2821 ttccctcgga tgtccgccgg cacgggatgg tcgcttcggg caacgatgac atcgggctgg
     2881 atgccgatcg atcgcaggtc acggacgctg tgctgcgttg gcttcgtctt cagctcctgg
     2941 gtcgcggcaa tgtagggaag gaaggtgacg tgaatcgaga gcgtatcctc ccgagcttcc
     3001 tcatgccgca tctgtcgaat ggcttcgagg aagggctggc cctcgatgtc gcccaccgtg
     3061 cccccaactt cgatgatgag gacatcggcg ccgctcgtct tcccgagcaa ccggatgcgt
     3121 tgcttgatct cgtccgtaac gtggggaatc gtttggatgg tgcggccgag gtaatcgcct
     3181 cgtcgctccc gagcgatgac ggcctggtgg atctggccgg ccgtgaccga gctgagccgg
     3241 gtcagctccg cgtcgatgaa tcgctcgtag tggcccaagt ccaggtccgt ctccgcgccg
     3301 tccatcgtca cgaagacctc accatgctga tagggcgaca tggtgcccgg gtcgacgttg
     3361 aggtagggat cgagcttctg gatggagacg gagaggctgc ggctcttgag gatccgcccg
     3421 agggccgctg tggtaatgcc tttgccgacg gagcttacga cgccgccggt cacgaagatg
     3481 tacttcggca tgaaccccgt tgaaatgagt aacggcgaag cgaccgcttc gccccactta
     3541 tcctacgtct ttcgggcaaa gggcgtcaac tgttgatccg acgattgttg tcgatgggac
     3601 gatggccttg tcgagcctcc tgcgagcctc gctcggcgtc ccgttccgcc agatgctggg
     3661 accaaagctc ctgaacgagg cgccgcgcgg actgataccc gagcacgtcg ccgagccagc
     3721 cgcccaggat ggcggccaag ccacgcgttc caaagagaag cacgacgagc gcggtgagct
     3781 tccaccaccc gggcaagacg atcagcatgg caatggcgat cgccgtgccg agcacgaggc
     3841 cggtgtaggc gaaggaatga acgatccagc tttcgcggcg ccgtttggct ttcacgatgg
     3901 ccaggacctg cggcgggatc agatcgacag ggcttcgttc ggtcgtcgat gtgccacagt
     3961 acggacaaag ctctcgatcg gcgcgttgag gtgtgtagca gttgccgcag aagcggcccg
     4021 cgttcggtac ctcgccctta tttatctggg tggcgacctc ctcgagacga ggggagatgg
     4081 gaaggtcctc gtaggtccgc tcgaggagtc ctgcgtgctc catcgtcgcc accattgcgc
     4141 ggttatacct cttcggacga gaaatccgga tcctgcatca gcgtaacgag gtagacgatg
     4201 gcgaggagta tcacgaggtt cgcagcgctc acgatgatcg acagacggcg gagcgaggct
     4261 tggtcttcgg ccggtgcgcc atcccgctgc tcgtcgagca tgcgtggccc gagcacgaac
     4321 gagtgccagg cggtgagcac aaccacgatg gcgaccaaga cgagcttcgc ggtgagcagc
     4381 cacgcgtacc ggtagtcgaa atcgaaaacg tcgcgcccga agaactcgag gtcgctctgg
     4441 cgctggaacg tgtttccgat tcccgtcacg agcaggatga ccagggatcc ccaccccagg
     4501 tatccgaagc gccgggtgat accagacatg acgttgctcc gcaccgactc gctgtcgatt
     4561 tc
//
LOCUS       DATMBB010000377         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_299515, whole genome shotgun sequence.
ACCESSION   DATMBB010000377 DATMBB010000000
VERSION     DATMBB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299515"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..915)
                     /gene="trxA"
                     /locus_tag="VNL92_08035"
     CDS             complement(67..915)
                     /gene="trxA"
                     /locus_tag="VNL92_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWO94698.1"
                     /translation="MATQPAVLDVTDETFRQTVLDQSHAKPVVVDFWAPWCGPCRMLS
                     PIIERVASSFGGEVVLAKLNTDENPRTAAEFGIRSIPAVKAFRQGRVVDEFVGALPEP
                     QVRAFFQKLAPSPAEQMASQADALARAGDLAGAERLYREVLQSAPNHADAIVGLARIL
                     IGRGEVGEAEGLLKRVPTDRRAKVLRHRLFLDGFAERHGNEDLERQARENPADPRARY
                     RWGVMLAARERYDEALAELLESVRLDPNFADGAARQAILAVFDILGLDSPLTREYQRH
                     LASLLF"
     gene            complement(1033..2283)
                     /locus_tag="VNL92_08040"
     CDS             complement(1033..2283)
                     /locus_tag="VNL92_08040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWO94699.1"
                     /translation="MAEMREAVVVDAVRTPFGRAGEKGVFRDITNVELVVPILKAIIE
                     RNKLDANLIDEIVMGSVGLAGPLTRARHYLFEADLPQTISGTDLNKQCGSSLQAFVQG
                     AQAIMTGMSDVVLAGGVETMGRVGPVTPTEKGEGMVPGGLPGEGGAPKAELPPDWKEA
                     ELLPTWPKKVEPWIMNMGMTAEKLAKEYKVTREDSDKFALRSHQLTIKAQQEGFFSDQ
                     IVPITIKYKDGTSVTVTEDQGPRADTSLEKLATLKPAYVEGGQVTAGNACPRNDGASV
                     VLIMEKNKARELGLKPLATFRHAATIGVDPTIMGIGPVPATRKLFQRTGLSIDQMDVI
                     ELNEAFACQAVAVQRELNAPDEKFNPHGGAVALGHPLGASGGRLVAQVARELNRTNGR
                     LGLATLCMGGGMGMSVVLEREDYW"
     gene            complement(2359..>2545)
                     /locus_tag="VNL92_08045"
     CDS             complement(2359..>2545)
                     /locus_tag="VNL92_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94700.1"
                     /translation="VIEGADFESGVVACGQAVGLASDIPSAGELVERIVREAREALDA
                     LLRSVAELPFHGEVSPR"
BASE COUNT          411 a          886 c          789 g          459 t
ORIGIN      
        1 tcggtgaacg tgccgcccac atcgacgccg aggatggagg ccatgtcccg gcgattatat
       61 cggcagctag aacagcaggc tggcgaggtg gcgctggtac tcgcgggtca gcggtgaatc
      121 gaggccgagg atatcgaaga ccgccagaat cgcctggcgt gccgccccat ccgcgaagtt
      181 cggatcgaga cggaccgact ccagcagctc ggccagcgct tcatcgtagc gctcgcgggc
      241 agcaagcatc actccccagc gataccgtgc ccgcgggtcg gccggattct cccgcgcctg
      301 gcgctcgagg tcctcgttac catgcctctc ggcgaacccg tcgaggaaga gacggtgccg
      361 tagcactttg gcccggcggt cggtgggcac gcgcttcagc agaccttccg cctcgccgac
      421 ctccccgcga ccgatcagga tgcgggccaa cccaacgata gcgtccgcgt ggttcggggc
      481 cgactgaagg acctctcgat acaggcgctc cgccccggcg aggtcgccgg cccgtgccag
      541 tgcgtcggcc tgcgaagcca tctgctcggc aggggaaggg gccagcttct gaaagaaggc
      601 gcggacttgc ggctccggca gcgctccaac gaactcgtcg acaaccctgc cctgtcggaa
      661 ggccttgacg gcagggatgc tgcgtattcc gaactccgcc gccgtgcgcg gattctcgtc
      721 ggtgttcagc ttggccagca caacctcgcc cccgaacgat gaagccaccc gctcgatgat
      781 ggggctcagc atgcggcatg gtccgcacca aggcgcccag aagtcgacga cgaccggttt
      841 cgcgtgcgac tggtcgagga cggtctgccg aaaggtttcg tctgttacgt caagaactgc
      901 cggctgggtt gccatcgatg atgctgcctc cttgtcctgc ttcgattcga ttgtagcagg
      961 agcggcgccc ctgagacgcc cggaggccgg ctcgtgggag ccggcctccg attcatcgcc
     1021 gaagcgcgtc gcttaccagt agtcctcgcg ctcgaggacg acggacatcc ccatgccgcc
     1081 acccatgcag agcgtcgcga ggcccagccg cccgttcgtg cgattgagct cgcgcgccac
     1141 ctgcgccacg agcctgccac ccgaagcgcc gagcgggtgg cccagcgcga cggcgccccc
     1201 gtgcgggttg aacttctcgt ccggggcgtt tagctcccgc tgaaccgcga cagcctgaca
     1261 cgcgaaggcc tcgttcagct cgatgacgtc catctggtcg atgctgagcc ccgtccgttg
     1321 gaagagcttc cgcgtggccg gaacgggacc gatccccatg atcgtggggt cgacgccgat
     1381 ggtcgcggcg tggcggaagg tcgcgagcgg cttcagcccg agctcgcggg ccttgttctt
     1441 ctccatgatg agcaccacgg atgccccatc gtttcgagga caggcgttgc cggccgtaac
     1501 ctgcccaccc tcgacgtagg ccggcttcag cgtcgcgagc ttctccaacg aggtgtcggc
     1561 ccgcggacct tggtcctcgg tgacggtgac cgacgtgccg tccttgtact tgatggtgat
     1621 cgggacgatc tgatcgctga aaaagccctc ttgctgagcc ttgatcgtca actgatggct
     1681 gcggagcgcg aacttgtccg aatcctcgcg agtaaccttg tactccttgg ccagcttctc
     1741 ggcggtcatg cccatgttca tgatccaggg ctcgaccttc ttcggccacg tcgggagaag
     1801 ctccgcttcc ttccagtccg gcggcaactc tgccttcggg gcgccgccct cgcccggcag
     1861 gccgcccggc accatgccct cgcccttctc cgttggcgtc acgggtccca cgcgacccat
     1921 cgtctcgacg cctccggcga gcacgacgtc ggacatgccg gtcatgatgg cctgcgcgcc
     1981 ttggacgaag gcctgcagcg acgagccgca ctgcttgttc aggtcggtgc ccgagatcgt
     2041 ctgcggcagg tcggcctcga acaggtagtg gcgcgcccgc gtgagcgggc cggccaagcc
     2101 caccgacccc atcacgatct cgtcaatgag gttcgcatcg agcttgttcc gttcgatgat
     2161 ggccttgagt atcgggacga cgagctcgac gttcgtaatg tcccgaaata cgcccttttc
     2221 gcctgcccgg ccgaacggcg tccggacagc gtcgactact acggcttcac gcatctctgc
     2281 catggatgaa ctcctttccc ttcctctcct ctcactcttg aaatcgaaac ggcgttagta
     2341 tacgccgctc acgagggctt atctaggaga gacttctcca tggaacggca actcggcgac
     2401 actcctcagc agcgcgtcga gcgcctctcg cgcctcccgc acaatccgct cgacgagctc
     2461 gccggcgctg ggaatgtcgc tcgccagtcc caccgcctgt ccacacgcaa cgacgccgct
     2521 ctcgaagtcg gcaccctcga tgacg
//
LOCUS       DATMBB010000378        11971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_576333, whole genome shotgun sequence.
ACCESSION   DATMBB010000378 DATMBB010000000
VERSION     DATMBB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576333"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1465
                     /locus_tag="VNL92_08050"
     CDS             239..1465
                     /locus_tag="VNL92_08050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWO94701.1"
                     /translation="MTTAAYEASIPEAIKGIKVIDVDTHFTEPPDLWTSRAPAKYKDH
                     VPYVKRTDGVDQWFFADKPMGPVSITVISKGHKKNYGIVTLDRYDEVDEATYDPAART
                     ALMDEMGVWGQIVFPNAAGFSGNNFATLVPEDMRNLCVRMYNDAISDWQQASGNRLFP
                     QALVPFWDIKEATREAQRIKEELGLTGIAAASAPETWGLPDYGDTAWDPFWEVCSELN
                     IPINFHIGGGAVDGGMRWKSFGLNALEPGQFAAARGTRMMAPIAAGALMGALMNARVL
                     INLIYSGMLDRWPKLKFVSIESGVGWIPFFLEMAEYQFDEMTSDDRWGLKLRPREYFQ
                     RHMYASWWFEDFGPRHALRTIGINNVLFETDFPHPTCLYPGTMKHLVDVLQDYTWEER
                     KRILQDNAVELYNLPV"
     gene            complement(1586..2560)
                     /locus_tag="VNL92_08055"
     CDS             complement(1586..2560)
                     /locus_tag="VNL92_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWO94702.1"
                     /translation="MNKDVHVTQLRALDAISRLGSYTRAAEELAYTEPAVHQQIRNLE
                     RTFGFGLVERRGQKMELTAEGEQILPAVLEVLDRFGSLEHAVRVIRDPDRVVVGAGRH
                     TGVFKIMPLLSDLRRAGIDIDVDLRILGPDDMRAGAMSGQIDVIVGGTLPDYFDHEWL
                     VQHRMVLVPWARETPALVGNTPDGHRQPNSSEPRIVFLPPYGTSRRSMVLSDLKRHLG
                     HTARFVEMGSADAAKGAAINGLGLAWLPVSSIADELEDGRLFLLYVPEPRRRRVYLLH
                     PRVRLLHKAARDLIKAFIRMERSRSPLKIGPEGQASQPVTVPPSPLFQ"
     gene            2738..3973
                     /locus_tag="VNL92_08060"
     CDS             2738..3973
                     /locus_tag="VNL92_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWO94703.1"
                     /translation="MTKVSRERPLGAYRVVELGGVAPMMAGKTLADLGADVVRVEPPC
                     GDPGRALPPRGRGNHDGTLWTATNLGKRSVTADLSHTEGCELVRELIERSDVLIEDFP
                     PGMLESRGMGPDALRRDRPKLIVASITTFGRTGPRASCTGSALVSLATGGYLYMTGPS
                     DGPPLKPSAPYQSEFHAGMHAAAAILLALRRRRRSGHGAHIDLAIRETATWMLTHTYQ
                     FYDLAGVNLRRQGSGRDMGGGARLRSVFEAADGYIVWMFMTGHIGGRNIKELIAWMGD
                     EHASPWLREIDWDEFDLRSAGPEILRELDAQFARFFKTKSREELTAWALDCGVMLSPV
                     QTLAEVLDDPQLAARDAWRTMAQAGGPRVPGPPVRMSGARWEPRGEAPSPGAHNSEVY
                     GMMGVDASRLDDLRERDVI"
     gene            3976..5217
                     /locus_tag="VNL92_08065"
     CDS             3976..5217
                     /locus_tag="VNL92_08065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWO94704.1"
                     /translation="MNDDGAINAQLELPLAGIRVLDFGTTVAGPTAARHLSDYGAEVI
                     KIESEVHPDTLRVGAPYAEGKPGINRSGYFAVYNAGKLSFALNLRIPESHAILRMLVE
                     RSDIVIETFRPGVMDRWGIGYEQMREWNPAIIMASHSLQGQWGPRAKHRGYGQLASAV
                     GGWYELTGMPNAPPMGPYSAYTDFIAWPFLLVAILTALEVRDVTGVGQRIDHSQVESS
                     IHFLAPLLLDLQLTGTTYSRRGNREDYAAPNNAYPCDGDDQWIAITVLDDEQWRALCD
                     VLERPEWSDDIRFASPSARIHHADDLDVLIAERTRGRHPQELAETLQARGVAAGVVAK
                     AQDLFEDEQLQHRQLFRRLDHPEIGSHAVLTSTFHIDGMASGPHRAAPLLGEHTHQVC
                     RDVLGLEEEEIARYAALGVFY"
     gene            5255..6412
                     /locus_tag="VNL92_08070"
     CDS             5255..6412
                     /locus_tag="VNL92_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWO94705.1"
                     /translation="MRLMLTEDQEQFKAEVEAFLDREWRPDPAAGGFDQPREMWERNQ
                     QFRRKLADRRWLTLAWPTEWGGGGASPLKQAIYNEVMSYRGAPSIDMGADRVGPAIMM
                     FGTDEQKHEFLPPITSATVNWCQGFSEPGAGSDLAGLQTRAIAEGDSFIVNGQKIWTS
                     NAHHAEWMMLLVRTDVEAPKHRGISVLLLDMKTPGIEVVPLINMAGGHGFNEVFFNDV
                     RVPRNRLLGELNRGWYVTATTLDFERSGIARIAPAKRYLDQIRTLLAEDPSLLTPRRR
                     LEFADLHIEYEVGRLLAYRVAWMQENGLVPNHEASMSKAYGSEVQQRVARFAVNLVGL
                     GGQLRPGSPHASIDGLPATSYMGTTALSIAGGTSEVQRNIIATRGLNMPRG"
     gene            6451..7323
                     /locus_tag="VNL92_08075"
     CDS             6451..7323
                     /locus_tag="VNL92_08075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWO94706.1"
                     /translation="MPSESLQGQCVLVTGATQPVGRAIAAAVAADGANVVVAGRVSDE
                     IGREALHTVLAEIRSLGAEAMAAELGEPDEEQIAACQTAIDERWGGLDVLINTAELSD
                     WSGPSWEMPTDEWDSVFSTNVHSALLLCRQFLPGMMERARGAIVNIGGWRWATPGLAA
                     FGASKASLDYLTMALAGEVRAFNIAVNSVSPSQGIDYPGSPHWRQQPGVPTAPEPPEH
                     FALAVVWLAKQDAESCTGNLTWSRQITSRNGLCRSWCCKPAQIVGPWRVAGRGHFPMN
                     SAPQRISLGVARDD"
     gene            7378..8328
                     /locus_tag="VNL92_08080"
     CDS             7378..8328
                     /locus_tag="VNL92_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWO94707.1"
                     /translation="MTGSLVGKVAVVTGAARGLGEAIAIAYGREGAKVVVVDRTTEPF
                     SRQGIVSEGTIHRTAEIIRSVGGEALPVKVDLRDEAEIEDCARIVADTYGGADIVVNN
                     AAIAYTARVKDMPLARWVQSYAVNSRGTFLMCKHFVPMMLPKGRGSIINLMTGRWAFP
                     GFGAYSSSKAAVDHFTLSLSEELRHFNIAVNCVAPSQGFRTPAGSYWQGGEAGQGGDP
                     SQMETQEPPEHAAKAFVWLARQDASTFTGNLVWSRPLIARYDLCDEWCCRGTIIGDRW
                     RVEPTGRFPAGVPSNLGTVPPIDEAAAAEESTEASTENAY"
     gene            8710..11763
                     /locus_tag="VNL92_08085"
     CDS             8710..11763
                     /locus_tag="VNL92_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWO94708.1"
                     /translation="MSDLFFEQPILNSPYEYPRRHWELDANGQPTQKIINTRRRAEFI
                     TPIPKPKKQKAASQQQRMVFNEGQGLSTEGREYDPTSIINEIRQHVDQWRLLPSSQCG
                     VTPETARLLDHWRHYEFGQIRPFFCQVEAVETAIWLTEVAPNTVRGKAILAHLAAANK
                     DAHPDLFRIALKLATGAGKTTVMAMLIAWQTVNAVRRPGSSRFTRGFLVVTPGLTIKD
                     RLRVLKPNDPDSYYRTLELVPPDMLEDLSKAKVVITNYHAFKLRERIEVSKAGRALLQ
                     GRRGDEIKTLETEGQMLQRVMPELMGMKNVLVLNDEAHHCYREKPDTDEEGALKGEDK
                     EEAKKNNEAARIWISGLEMVKRKLGISSVVDLSATPFFLRGSGYAEGTMFHWTVSDFS
                     LMDAIECGIVKVPRVPVADNIPNQQMPMYRNLWEHIRTKMPKKGRGKASELDPLSIPP
                     QLQTALDALYGHYEKVFQEWKDAGIRVPPCFIVVCNNTSTSKLVYDYISGFERQNEDG
                     STQLVPGRLELFRNFDEHGNPLSRPKTLLIDSEQLESGEGLDDNFRTMAADEIERFRR
                     EIIQRTGDAQAAENITDVELLREAMNTVGKPERLGASIRCVVSVAMLTEGWDANTVTH
                     ILGVRAFGTQLLCEQVIGRALRRQSYDLNEDGLFNVEYADILGIPFDFTAKPVVVKPQ
                     KPRETIQVRAVRPERDALEIRFPRVAGYRVELPNERLTANFTKDSVLRLTPDLVGATA
                     TRNSGIIGQDVDLTLAHNADVRPSQVLYELTSHLVLTKWRDPGGDAKLNLFGQLKRIA
                     KEWLDNYLVCEGGTYPAQLKYKTLADMACNRITDAITREFVGEHPIIAVLDPYNPTGS
                     TMHVNFATSRLSRWETDSRKCHVNWVILDSDWEAEFCRVAEAHPRVKAYVKNHSLGFE
                     VPYLYGSEPRKYRPDFIVLIDDGHGDDDPLHLVVEIKGYRGEDAKEKASTMKTYWVPG
                     VNNLKEHGRWAFAEFTSVYAIQSDFASKVAGLFDEMVESVRPKLS"
     gene            11779..>11971
                     /locus_tag="VNL92_08090"
     CDS             11779..>11971
                     /locus_tag="VNL92_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94709.1"
                     /translation="MDLSQAEADALLAMEKIAADSDVHEFPGIATGVIIRLVSKVGRE
                     EFFLDIKRGRIDLAKATYQN"
BASE COUNT         2375 a         3722 c         3805 g         2069 t
ORIGIN      
        1 tcgaggccgt tcatcagctc cggggcgacg cgggcgagcg acagcgaccc aatgctgagg
       61 tcggcatggt gtcgaacgcc gggctcaact tgatgggcag cgcggtaatc ctgcggcggt
      121 aggcaactta ataaagtaga gccggaaggg agggtaggga ttgcgcgatt ttagagagag
      181 atgcaatgag gttgcactcg tcgtccgttg tgtagcctga atgtactgga ggtaattcat
      241 gacaaccgca gcttatgaag catcgatccc agaggcgatt aaaggcatca aggtcatcga
      301 tgtcgacacg cactttacgg agccgcccga tctgtggaca tcgcgcgcac ctgccaagta
      361 caaggaccac gtcccgtacg tgaaacgtac cgatggcgtc gaccagtggt tcttcgccga
      421 caagccgatg ggcccagtct cgattactgt cattagtaag ggccacaaga agaactacgg
      481 aattgtcaca ctcgatcgtt acgacgaggt cgacgaggcg acttacgacc ctgcagcgcg
      541 cacggcgctg atggacgaga tgggcgtttg gggccagatc gtcttcccga atgccgcagg
      601 gttctccggc aacaacttcg ccacgctcgt gccggaggac atgcgcaatc tctgcgtgcg
      661 catgtacaac gatgccatct ccgactggca gcaggcctcg ggcaaccggc tcttcccaca
      721 agcgcttgtg ccattctggg atatcaaaga ggccacccgt gaggcacagc gcatcaagga
      781 ggagcttggg cttaccggaa tcgccgcggc gagcgcccct gagacgtggg ggctccccga
      841 ctacggcgat actgcgtggg atccgttttg ggaagtctgc agcgagctca acatcccgat
      901 taacttccac atcggtggcg gcgcggtcga tggcggtatg cgatggaagt cattcgggct
      961 aaatgccctc gagccggggc agtttgcggc ggctcgtgga acgcgcatga tggcgcctat
     1021 cgccgccgga gcgctgatgg gtgcacttat gaacgcccgt gttctcatca accttattta
     1081 cagtggcatg ctagaccgct ggccgaagct caagttcgtc tccatcgaga gcggcgtagg
     1141 ctggattccc ttcttcctcg aaatggccga gtaccagttc gatgaaatga ccagtgacga
     1201 ccgctggggg ctcaagctac gcccgcgcga gtacttccag cgccacatgt acgcgtcgtg
     1261 gtggtttgag gacttcgggc cgcgccacgc gctccgcacc attggtatca ataacgtgtt
     1321 gttcgagacc gactttccgc acccgacgtg cctctatcca ggcaccatga agcacctcgt
     1381 ggacgtcctt caggactata cgtgggagga gcggaagcgc attcttcagg acaacgctgt
     1441 cgaactctac aacctgcccg tgtaagggac cggtacgtcg ggcgtgaggt cggccgagcg
     1501 cggcgtccga ccgacagatc tagaggcgtc caagactggt catgcggggc ccgaccttcg
     1561 agtccgggcc ccgcatgacg gcgaatcact ggaacaacgg tgatggaggg accgttaccg
     1621 gctgtgaggc ctggccttca ggtccgattt tcaaaggcga gcgcgaacgc tccatgcgga
     1681 tgaaagcttt aatcaggtct cgggcagctt tgtggagaag ccgtactctc ggatggagga
     1741 ggtacactcg ccgtctgcgg ggctctggaa cgtaaagcaa gaacaggcgt ccatcttcga
     1801 gttcatcggc gatcgagctc acgggtagcc acgcgagacc gagtccgttg atagctgccc
     1861 ccttagccgc atcggcggag cccatctcaa cgaagcgggc agtatggccg agatggcgct
     1921 tgaggtccga gagcaccatg gaacggcggc tcgtcccgta cggcgggagg aagacaatgc
     1981 gcggttcaga agaattcggc tgtcggtgtc cgtccggtgt gttgccgacg agggctggag
     2041 tttccctggc ccacgggacc agtaccatcc gatgctgcac aagccactcg tgatcgaaat
     2101 agtccgggag ggtacccccg acgatgacat cgatctgacc gctcatggcg ccagccctca
     2161 tgtcatccgg accgaggatc ctcaggtcga cgtcgatatc gatcccggcg cgcctcaggt
     2221 ccgagaggag cggcataatc ttgaacactc cggtgtgccg cccagcaccg actacgacac
     2281 gatctgggtc gcgaatgacc cgcacggcat gctcgaggga gccgaagcgg tccagcactt
     2341 ccaagaccgc gggcagaatc tgctcacctt ccgcggtgag ttccatcttc tgcccgcgcc
     2401 gctcgaccag accaaagcca aacgtgcgct cgaggttccg gatctgctga tggacggccg
     2461 gctccgtata cgcaagctcc tcggcggcgc gagtgtagct ccccagtcgg gatatcgcgt
     2521 cgagggcccg gagctgcgtt acgtgcacat ccttgttcat ccgctgctct ccaacctccc
     2581 agggacacac tgcatgcgtt acgcatactc agaagacggg agcagatgat ttcatgcgag
     2641 ccggaatgcc gtcaagcgtc atttttgcta tcgaagtttc tctactccga ctccgcggac
     2701 gccacgcgat cgcggccgtt cccgaacagt cgggcggatg accaaggtga gtcgcgaaag
     2761 gccgctcggg gcctaccggg ttgttgaact cgggggagta gcgcctatga tggccggcaa
     2821 gacacttgcc gaccttggcg ccgacgtcgt cagggtcgag ccaccgtgcg gcgacccggg
     2881 gcgcgcccta ccgccgagag gacgcggcaa ccacgatggc actctctgga cggcaacgaa
     2941 cctcggcaag cgaagcgtta cggccgacct aagtcacacc gagggctgcg agctcgttcg
     3001 ggagctgatc gagcggtccg acgttctcat cgaggacttc ccccccggga tgctggagtc
     3061 acgtggcatg ggcccggacg cgttgcgccg ggatcgaccg aaactcatcg ttgcgtcgat
     3121 aacgacgttt ggtcgcaccg gtccccgcgc atcgtgcacc gggtctgccc tggtcagctt
     3181 agcgacgggc ggctacctgt acatgacggg cccgagcgac gggccaccgc tgaaaccttc
     3241 ggcaccctat cagagcgagt tccacgccgg catgcacgcg gcagcagcta ttctgctcgc
     3301 gctgcgtcgt cggcgccgtt cggggcacgg cgctcacatc gacctggcaa tacgcgaaac
     3361 ggccacctgg atgctcaccc acacctacca gttctacgat ttggcgggcg tcaacctgcg
     3421 gcggcagggc tcgggccgcg atatgggcgg cggcgccagg ctccgctcgg tcttcgaggc
     3481 agcggatggc tacatcgtct ggatgtttat gaccggccac atcggcggac gcaacatcaa
     3541 ggagctcata gcctggatgg gcgatgagca cgccagtccg tggctgcgcg agatcgactg
     3601 ggacgagttt gacctgcgct cggcggggcc tgagatcctg cgtgaactcg atgcccagtt
     3661 cgcgcggttc ttcaagacga agtcccgcga ggagctcaca gcgtgggcac tcgactgtgg
     3721 agtcatgctc tcccccgtac aaacgctcgc cgaggtgctc gatgatccgc agctcgccgc
     3781 ccgcgatgca tggcggacca tggcgcaggc cggagggccg cgggtgcctg gtccaccggt
     3841 ccggatgagc ggcgcgcgat gggagccgcg cggcgaggcg ccgtcaccag gtgcgcataa
     3901 ctccgaagtc tacggaatga tgggcgtcga tgcctcgagg ctcgatgacc tgcgggagcg
     3961 agatgtcatt tgagcatgaa cgacgacggc gcgatcaatg cacagctcga gctgccgctc
     4021 gctggaattc gggtcctcga ctttgggacg accgttgcgg gcccaacggc cgcgcgccac
     4081 ctctcggact acggcgccga ggtcatcaag atcgagtcgg aggtacatcc ggacacgctt
     4141 cgggtcggcg cgccgtatgc ggaaggcaag cccgggatca accgttccgg ctacttcgca
     4201 gtgtacaacg ctggcaagct ctcgttcgcg ttgaatctgc gtatccccga atcgcacgcc
     4261 atcctgcgaa tgctggtcga gcgatccgac atcgtaatcg agacgttccg accgggcgtg
     4321 atggaccgct gggggatcgg ctacgagcag atgagggagt ggaatccagc catcatcatg
     4381 gcgagccaca gcctccaggg acaatggggg ccacgcgcca agcatcgcgg ctacgggcag
     4441 ctggcgagcg ctgtcggggg gtggtacgag ctgacaggca tgcccaatgc gccaccgatg
     4501 ggaccgtact cggcctacac cgacttcatc gcatggccgt tcctgctcgt tgccatcctc
     4561 acagccttag aggtccgtga cgtcacggga gtcgggcagc gaatcgacca ctcgcaggtc
     4621 gagagctcga tccacttcct cgcgccgctc cttctcgatt tgcagctgac gggcacgacc
     4681 tacagtcggc gcggaaatcg cgaggactac gccgcgccca acaacgccta tccctgcgat
     4741 ggcgacgatc agtggatcgc gatcactgtc ctggatgatg agcagtggcg ggcgctctgc
     4801 gacgtgctgg aacgccccga atggagtgat gacatccgct tcgcgtcgcc cagcgctcgc
     4861 atccaccacg ccgatgacct tgacgtgctg atcgcagaac gaacgcgcgg gcgacatccg
     4921 caagaattgg ctgaaacgct acaggcgcgg ggcgtggccg cgggcgtggt cgcgaaggcg
     4981 caggacctgt tcgaggacga gcagctgcag caccgacagc tctttcgccg tctcgatcac
     5041 ccggagatcg gctcgcacgc tgtcctcacg tcgacgttcc acatcgacgg catggcatca
     5101 gggccgcacc gcgcagcacc gctactaggc gagcacacgc atcaggtgtg ccgtgacgtg
     5161 ctcggcctcg aggaggagga gatcgctcgt tatgccgcgc tgggcgtctt ctactgaggc
     5221 acctgaccag aaccaacgga atgagaggac tgttatgcga ctcatgctga cggaggacca
     5281 ggagcagttc aaggccgagg tggaggcctt cctcgaccgg gaatggcgtc ccgatccggc
     5341 cgcgggcggg ttcgaccagc cgcgcgagat gtgggagcgc aaccagcagt tccggcgcaa
     5401 gctcgccgat cggcgctggc tcacactcgc ctggccaacc gaatggggag gcggcggagc
     5461 gtccccgctt aagcaggcca tctacaacga ggtcatgagc tatcgtggtg caccgagcat
     5521 tgacatgggg gcggataggg tcggaccggc gatcatgatg ttcgggacgg acgagcagaa
     5581 acacgagttc cttcccccca tcacgtcggc gacagtgaac tggtgccagg gcttctccga
     5641 gccaggggcc ggctcggacc tcgccggcct ccagacccgc gctatcgccg aaggcgactc
     5701 cttcatcgtc aacgggcaga agatctggac cagcaatgcc caccacgccg agtggatgat
     5761 gctgttagtg cgaaccgacg tagaagcgcc gaagcatcgc ggcatctcag tgctcctgct
     5821 cgacatgaag accccgggca tcgaggtcgt gccgctcatc aacatggccg gcgggcacgg
     5881 cttcaacgag gtcttcttca acgatgtccg cgtcccgcga aatagactgc tgggcgagct
     5941 aaaccggggc tggtacgtca cggcaactac actcgacttt gagagatccg gcatcgcgcg
     6001 catcgctcca gcgaagcgct acctcgatca gattaggact ctgctggccg aggatccgtc
     6061 gttgctcacg ccgaggcggc gcctcgagtt cgccgacctg catattgagt acgaggtcgg
     6121 acgattgctc gcctaccgcg tcgcgtggat gcaagagaac ggactcgtgc cgaaccacga
     6181 agcgtcaatg tcgaaagcgt atggatcgga ggtgcagcag cgcgtggccc ggttcgccgt
     6241 caatcttgtg gggctcggtg ggcaacttcg gccgggctcg ccgcacgctt cgatcgacgg
     6301 cctgccggca acgagctaca tggggacgac ggcactttcg atcgccggcg gcacgagcga
     6361 ggtccagcgg aacatcatcg cgacacgcgg cctcaatatg ccgcgcggct agggctgagt
     6421 tgatcagcag tttagtaagg ggagattacc atgccgagcg aatccttaca gggccaatgc
     6481 gttctggtga cgggggctac gcagcccgtc ggtcgggcga ttgctgcggc tgtggcggcg
     6541 gacggggcca acgttgtcgt cgcggggagg gtcagcgacg agatcggccg tgaagcgctg
     6601 catacagtac ttgccgagat ccggtcgctg ggcgcagaag cgatggctgc ggagctcggc
     6661 gagccggacg aggagcaaat cgccgcgtgc caaaccgcga tcgacgagcg atggggcggg
     6721 ctcgacgtcc tcatcaacac agcagagctc tcggactgga gcggtcccag ttgggagatg
     6781 ccgaccgacg agtgggactc ggtgttctca acgaacgtcc acagcgcgtt gctgctctgc
     6841 cggcagttcc taccgggcat gatggagcga gcacgcggcg ccatcgtcaa cattggaggg
     6901 tggcggtggg cgacacccgg actcgcggcg ttcggcgcat ccaaggcgtc tctcgactac
     6961 ctcacgatgg cgctcgctgg cgaagtgcga gccttcaaca tcgccgtgaa cagcgtctcc
     7021 ccgtcccagg gcattgatta tcccggttca ccccactggc ggcagcagcc gggcgtgcca
     7081 acggcgccgg agccgccgga gcactttgcg ctggccgtcg tctggctggc gaaacaggat
     7141 gcggagagct gcaccggcaa tctcacgtgg agccgtcaga tcacctcccg gaacgggctg
     7201 tgccgaagct ggtgctgtaa gccagcgcaa atagtcggcc cgtggcgcgt cgccggccgg
     7261 ggccactttc cgatgaacag cgcaccgcag cgcattagcc tcggcgtggc gagggacgac
     7321 taaggcttgc gcagccgaac gtcgcatctc cgacgctgca gaaaggagca tgcaccagtg
     7381 accgggtcgc tcgtagggaa agtcgccgtc gtcacggggg ccgcgagagg cctcggcgag
     7441 gcgatcgcta ttgcgtacgg acgcgaaggg gcgaaggtcg tcgtcgtgga ccgcaccacg
     7501 gagccgttct cgcgtcaggg catcgtctca gagggaacga tccaccggac ggctgagatc
     7561 attaggagtg tcggaggcga ggcactgccg gtgaaagtcg accttcggga cgaagccgag
     7621 atcgaagact gtgcgcgcat cgtcgccgac acgtacggtg gggccgatat agtggtcaac
     7681 aatgcggcga tagcttacac ggcgcgcgtg aaggacatgc cgctcgcgcg ctgggtacag
     7741 agctacgcgg tgaacagccg cggcacattc ctgatgtgca agcatttcgt gcccatgatg
     7801 ctgccaaaag ggcgtggatc gatcatcaac ctcatgacgg gccggtgggc ctttcccggc
     7861 tttggcgcct acagctcctc gaaggccgcc gtcgaccact tcacactcag cctctcagaa
     7921 gaactgcggc acttcaacat cgcggtcaac tgcgtcgcgc cgtcgcaggg tttcaggacg
     7981 ccggcgggct cctactggca gggtggcgag gcagggcaag gcggcgatcc gagtcagatg
     8041 gagacgcagg agcctcctga gcacgctgcg aaggccttcg tttggctcgc ccgacaggac
     8101 gcatcgacgt tcacggggaa tctcgtgtgg agccgcccac tgatcgcgcg ttacgatctc
     8161 tgcgatgagt ggtgctgtcg cggcacgatc atcggcgacc gctggcgtgt agagccgacg
     8221 gggcgctttc cagcgggtgt cccatccaac ttgggaacag tgccgcccat cgacgaagcc
     8281 gcggcggcag aagagtcgac cgaggcatcg accgagaacg cctactgacg acgcgatgct
     8341 cgtggtccgg aagcgaattc acgaaccgcc tagagccgct ggtcgtcaca atgcggtgac
     8401 gcgtcgactc gtttatctgc gctcggattg gtgacatcgc taggatctcg cacgtcagtt
     8461 gtccaaaacc accaagggcg cgctgacccg gcctgaacag gaagaaggcg aggatactcg
     8521 ctcttttctt gccctgaaaa cgctcatggt ggttcgcggg ccatccaacg gcctgagcgg
     8581 tccacggtca ctgtgcttga gatcacctgc ccgctatgat agatagagcg cacatttggg
     8641 ctgcgccgat ctatcttcaa ccgtcggcgg gcatccgcac gcgcccccaa aaaaggtaag
     8701 atgtccgcca tgtccgacct cttcttcgaa cagccgatac tgaactcgcc gtacgagtac
     8761 ccgcggcggc attgggagct ggacgccaac ggccagccga ctcagaaaat cattaacact
     8821 cgtcgccgcg ccgaattcat cacccctatt ccaaagccca agaagcagaa ggctgcctcc
     8881 cagcagcaac gcatggtgtt caacgaaggc caggggctct cgaccgaagg ccgggagtac
     8941 gatcccacct ccatcatcaa cgagatccgt cagcatgtgg accagtggcg gctgttgccg
     9001 tcttcccagt gcggcgttac gccggaaact gcccggctcc tcgatcactg gcgtcattac
     9061 gagtttggcc agatccgacc gttcttctgc caagtcgagg cagtggagac agccatttgg
     9121 ctgacagaag tggctcccaa cacagtccga ggcaaggcga tcctggctca cctcgccgcc
     9181 gctaacaagg acgcccatcc tgacctgttc cgaatcgccc tcaaactggc caccggcgcc
     9241 gggaagacca ccgtcatggc gatgctcatc gcttggcaga ccgtgaacgc cgttcggcgg
     9301 ccggggagca gtcgttttac tcgcggcttt ctcgtcgtaa cccctggcct caccatcaag
     9361 gaccgcctcc gtgtcctcaa gccgaacgac ccggacagct attaccggac gctcgaactc
     9421 gtgcctccgg acatgcttga agacctgagc aaggcgaaag tcgttatcac gaactaccat
     9481 gccttcaagc tccgcgaacg catcgaagtg tccaaggctg ggcgggcgtt gctacaggga
     9541 cggcgtggcg acgagattaa gacgctggag acggaaggcc agatgctcca gcgggtcatg
     9601 cccgagttga tgggcatgaa gaacgtcctc gtcctgaacg acgaggctca tcactgctac
     9661 cgcgagaagc cagacacgga cgaggaggga gcgctcaagg gcgaggacaa ggaggaggcc
     9721 aagaagaaca acgaggccgc ccgcatctgg atctccggcc tggaaatggt gaagcgcaag
     9781 ctgggcatat caagcgtcgt cgacctgtcg gcgacgccgt tcttcctgcg cggttccggc
     9841 tacgcggaag gaacaatgtt ccactggacc gtcagcgact tctctctgat ggatgccata
     9901 gagtgcggga tcgtgaaggt gccccgggtg ccggtggccg acaatattcc caaccagcag
     9961 atgcccatgt accgcaatct ttgggagcat atccggacaa agatgcccaa gaagggccga
    10021 ggaaaggcca gtgaactcga ccccttaagc atccctccgc agctccagac cgctctggat
    10081 gctctttacg gacactatga aaaggtcttc caggaatgga aggatgcagg tatccgcgtc
    10141 cctccctgct tcatcgtggt ctgcaacaac acgtccacct ccaagctcgt gtacgactac
    10201 atctcgggat tcgagcgcca gaacgaagac ggctccacac aactggtacc cggccgcttg
    10261 gaactgttcc gcaacttcga cgagcacggc aaccccctat ctcgccctaa gacgttgctg
    10321 atcgatagcg agcagttgga gtccggcgaa gggctggacg acaacttccg caccatggcc
    10381 gccgacgaga tcgaacggtt ccgccgcgag atcatccagc gcacaggcga cgcgcaggcc
    10441 gcggagaata tcacggacgt tgaactgctc cgagaagcga tgaacacggt cgggaagccg
    10501 gagcggctcg gcgcctcgat ccggtgcgtc gtctccgtcg cgatgctcac ggagggctgg
    10561 gacgccaaca ccgtcaccca catcctcggg gtgcgcgcct tcggcacgca gctgctctgc
    10621 gagcaggtta tcggtcgtgc cctccgcagg cagtcctatg acctgaacga agacgggctc
    10681 ttcaacgtcg agtacgccga catcctgggc atccccttcg acttcacggc caagccggtc
    10741 gtcgtgaagc cccagaagcc cagggagact atccaagtac gggccgtccg ccccgagcgc
    10801 gatgccctgg agatccgctt tccgcgcgtg gcaggctacc gcgtcgagct gccgaacgag
    10861 cgcctcaccg ccaacttcac gaaggactcg gtgctccgcc tcacgcccga cctcgtcggg
    10921 gccacggcga cgcgcaactc cgggatcatc ggccaggacg tcgatctcac gcttgcccac
    10981 aacgccgatg tccgcccctc gcaggtgctg tacgagctca cgtcgcatct cgtcctcacc
    11041 aagtggcgag acccgggagg cgacgcgaag ctgaacctgt tcggccagct caagcgcatc
    11101 gccaaggagt ggctggacaa ttaccttgtc tgcgagggcg gcacctatcc cgcccagctc
    11161 aagtacaaga cgctggcaga catggcctgc aaccggatta ccgatgctat cacgcgggag
    11221 ttcgtcggcg agcaccccat catcgccgtg cttgatcctt acaaccccac cggctccacg
    11281 atgcacgtca actttgcgac gtccaggctc agccggtggg agacggattc ccgcaagtgc
    11341 cacgtcaact gggtcatcct ggacagcgac tgggaagcgg agttctgccg cgtcgcggag
    11401 gcgcatcccc gcgtcaaggc ctacgtgaag aaccacagcc tcggcttcga agtgccctac
    11461 ctctacggct cggagccgag aaagtatcgc ccggacttca tcgtgctcat cgacgacggc
    11521 cacggggacg acgacccgct gcatctggtc gtggagatca agggctaccg gggcgaagac
    11581 gcgaaggaga aggcttccac gatgaagacc tactgggtgc cgggcgtgaa caacctgaag
    11641 gagcacggac gctgggcgtt tgcagagttc accagcgtct acgccatcca gtcggacttt
    11701 gcctcaaagg tggccgggct gttcgacgag atggtcgagt ctgtgcgtcc gaagctgtcg
    11761 tgagggggag ttagtggttt ggatctaagc caagctgagg ccgacgcgct ccttgcgatg
    11821 gagaagatcg ccgcagactc cgatgtccac gaattccccg gcattgccac tggggtgatc
    11881 atacgcctcg tgtcgaaggt tgggcgcgag gagttctttc tagacatcaa gcgtggccga
    11941 atcgatttag ctaaagccac ttaccagaac a
//
LOCUS       DATMBB010000379         3608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_417067, whole genome shotgun sequence.
ACCESSION   DATMBB010000379 DATMBB010000000
VERSION     DATMBB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417067"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2242
                     /locus_tag="VNL92_08095"
     CDS             <1..2242
                     /locus_tag="VNL92_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWO94710.1"
                     /translation="MMNPAAKIVQAWHLTPVTTKGAARLSATLYLASGGLVILNQVLP
                     LPAGANRTGLVDVGALAVAIGVLIWMLPWQRWPRWSTLSLVAVALPIIVIHNHFTGSD
                     PYRYGVFYFVVLAWTGLCHPRGVASLVGVPVTFAYLVPLIVDGAPGAGLTSALYIVPV
                     SVLVGETLSWSMANLGHARDEIRSRDSFFRSLVSNSSDLITLIDADGVVKFQSPSAEH
                     LLGVNPVRTAGTLIDLHIHAGDRQRFRAALAQLPAYRGPLEVRWKAASGWQDLELTIS
                     DLRDHPDVRGIVLNGRDVSERRALQANLLHQAYHDALTGLPNRVLFTSELAAAFEQAS
                     RGGEACAVLIIDLDGFKHFNDRFGHGPGDRLLVEVGARLRAAVAERGLAARLGGDEYG
                     VLLPRHDADAALAFARELLGLLSAQHVIDTVSVSLSASIGIADATSGASPEDLVRNAD
                     IAMYAVKARGKADAVVFSPDMYETLVQRLELGGAIEDAIDAGQFVLHYQPIMDLKQRR
                     IIGAEALVRWNHPTRGLLPPGAFIELAEENRSIVALGRWVLNKACDQAQEWLARKLVD
                     RDFRMSVNVSVQQMAEPAFAGEVKQALERSKLLPTQLVLEITETTLMVRSETTMCTLN
                     ELRRLGVHIDIDDFGSGYSSLGYLAAFPVSGIKVDRSLVSRLGTSLEAAALMRGVVDL
                     GQSLGLEVTAEGVELPDQLREVESLAAPLAQGYLFSPPLPADDFVAYLESADLDAIED
                     IARTAA"
     gene            complement(2255..2776)
                     /locus_tag="VNL92_08100"
     CDS             complement(2255..2776)
                     /locus_tag="VNL92_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWO94711.1"
                     /translation="MFQHILIPLDGSTRSEAAIEHAVAIAQQFAGRITLLRAVQLPDA
                     ENISAENLDRIRDQQLGAARDYLEGWASKINQRGVQCQPIVVAGDPARSILGYAHRSN
                     VDLVVMNSHGAGGLSGYVFGSVADKVVRTASCPVLVLHVRPTEEELREQEEREVAEMD
                     ATLTAMLGRQPRA"
     gene            complement(2978..>3608)
                     /locus_tag="VNL92_08105"
     CDS             complement(2978..>3608)
                     /locus_tag="VNL92_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWO94712.1"
                     /translation="GPRLRREIPLPLPLSADEFEHALRGGALIIDVRSKEEYAEGHIP
                     NAIHIAFGDTYATWLGWLVPPEAQLLFVLDDAPLEDVFDESLLVGYEHLGGWLAGGVD
                     AWGRSGGELRRTATLDVRTARRQLADGVVALDVREPDEHDFGHIEGARHIPLGSLQEG
                     AATLPKDRPLLVYCAHGDRASTAISVLERAGFESIASLAGGYEAWRREA"
BASE COUNT          571 a         1187 c         1199 g          651 t
ORIGIN      
        1 catgatgaac ccggcggcca agatcgtgca ggcgtggcac ctgacgcccg tgacgaccaa
       61 gggggcggcg cgcctttcgg cgaccctcta ccttgcatcg ggcggcctcg ttatcctcaa
      121 ccaggttctg cccttgcctg cgggcgccaa ccggaccggg ctggttgacg tgggcgcgct
      181 tgcggtggcg atcggggtgc tcatctggat gctgccttgg cagaggtggc cgaggtggtc
      241 gacgctttcc ctcgtggccg ttgcgctgcc gatcatcgtc atccacaacc acttcaccgg
      301 gtccgatcca tatcgatacg gcgtgttcta cttcgttgtt ctggcatgga ccgggctgtg
      361 ccaccctcgc ggggtcgcca gcctcgtcgg tgttcccgtg accttcgcgt accttgtgcc
      421 gctgatcgtg gatggggcgc cgggggcagg cctcacgtcg gcgctctaca tcgtccccgt
      481 gagcgtcctt gtcggcgaga cgctgtcgtg gtcgatggcg aacctcggcc acgcgcgcga
      541 cgaaatacgc agtcgcgatt ccttcttccg gtcgcttgtc tcgaactcgt ctgacttgat
      601 tacgctcatc gacgctgacg gcgtcgtgaa gttccagagc ccatccgcgg agcacttgct
      661 cggcgtgaac ccggttcgga ctgccggaac gctcatcgac ctccacatcc atgccgggga
      721 tcgacagcgc ttccgcgccg cgctcgcgca gctccccgcg tatcgcgggc cgctggaggt
      781 gcgatggaag gcggcgagcg gctggcagga cctggagctg acgatatctg acctccgcga
      841 ccatcccgat gtccgcggca tcgtgctcaa cggacgcgac gtgagcgaac gcagggcgct
      901 gcaggcgaac ctccttcacc aggcatacca cgacgcgctc accggcctgc cgaatcgagt
      961 gctgttcacc agcgagctgg ctgcggcatt cgagcaagct tcccggggcg gagaggcttg
     1021 cgccgtgctg atcatcgatc tggatggatt caagcacttc aacgatcgct tcggccacgg
     1081 ccccggcgac cggctcctcg tcgaggtcgg tgcgcgcctg cgtgcggcgg tcgccgaacg
     1141 cggtcttgcg gcgcggctcg gcggggacga gtacggcgtg cttttgcctc gccacgacgc
     1201 agacgccgcg ctggcgttcg ctcgcgagct gctcggcctc ctatcggctc agcacgtcat
     1261 cgatacggtg tcggtgtcgc tctcggcgag catcggcatc gcggacgcga cgagcggagc
     1321 ctcgccggag gatctggtgc ggaacgcgga catcgcgatg tatgcggtga aggcgcgcgg
     1381 caaggccgat gcggtcgtct tctcacccga catgtacgag acgctcgtcc agcgactcga
     1441 gctgggcggc gccatcgaag acgccatcga tgcgggtcag ttcgtgctgc actatcagcc
     1501 aatcatggac ctgaagcaga ggcggatcat cggcgccgag gcgctcgtac gctggaacca
     1561 cccgactcgc gggctcctgc cgcccggcgc gttcattgag ctcgccgagg agaatcgctc
     1621 catcgtcgcg ttgggccgct gggttctgaa caaggcgtgc gaccaggctc aagagtggct
     1681 ggctcgcaag ctcgtcgacc gggacttccg gatgagcgtt aacgtctctg tgcagcagat
     1741 ggccgagccg gccttcgcgg gcgaggtgaa gcaggcgttg gagcgctcca agctcctccc
     1801 aacacagctt gtgctcgaga taacggagac gacgctgatg gtgcgctcgg aaactaccat
     1861 gtgcacgctg aacgagctgc ggcggctcgg ggtgcacatc gacatcgacg atttcggctc
     1921 gggctactcc tcgctgggat accttgccgc gttcccggtc agcggaatca aggtcgaccg
     1981 ctcgctcgtg tcgcgtctcg gcacgagcct ggaagcggcg gcgctgatgc gcggcgtggt
     2041 tgacctgggt cagtcgctgg gcctcgaggt gacggccgag ggagtggagc tgccggacca
     2101 gctccgcgag gtcgagagcc tcgctgcccc gctcgcgcag ggttacctgt tctcgccgcc
     2161 gctccccgcg gacgatttcg tggcctatct cgaatccgcc gacctcgatg cgatcgagga
     2221 tatcgcgagg actgccgcct aagaccgccg cgacctaggc tcgcggttgg cggccgagca
     2281 tagcggtcag cgtcgcatcc atctccgcga cttcccgctc ctcctgctcg cggagctcct
     2341 cttccgtagg ccggacgtga agcacgagca ccgggcagct ggccgtgcga accaccttat
     2401 cggcaacgct gccgaagaca tagcccgaga gcccgcctgc gccgtggctg ttcatgacga
     2461 cgaggtcgac gttgctgcgg tgcgcataac cgaggatgga gcgcgccgga tcgccggcca
     2521 cgacgatcgg ctggcactgc acgccgcgct ggttgatttt ggaggcccac ccctccagat
     2581 aatctcgggc cgcgccgagt tgctgatcgc gaatccgatc cagattctca gcagagatat
     2641 tttccgcatc aggcaactgg acggcgcgaa gaagcgtgat acgtcctgcg aattgctggg
     2701 cgatggcgac ggcgtgctct atcgctgcct cggagcgcgt cgacccatcg agtggtatga
     2761 ggatgtgctg gaacatgcct tctcccttca gttcgtgctt cgttccgcga ggcgccggtc
     2821 acgtaggcga gcgtatcgcg ttccaagcta cgttgccaga cacggcgacc cggcatgagc
     2881 gccaaacggc tgcgattcca tgggccgaat gtcgcagagg agtgctgtac ggcgatcggc
     2941 catcgcgcta tggaggctgt gctcggctag gagcttatca tgcctcgcgc cgccacgctt
     3001 cgtagccgcc ggccagacta gcgatcgatt cgaacccggc tcgctccagc actgagatgg
     3061 cggtcgacgc acggtcgccg tgggcgcagt acacgagcag ggggcggtcc ttcggcagcg
     3121 tcgcggcacc ctcctgcaag gatccgagag ggatgtgtcg ggctccctcg atgtggccga
     3181 agtcatgctc atccggctct cgaacgtcga gcgctaccac gccgtcggcc agctggcggc
     3241 gagccgtccg aacgtccagc gtcgcggttc gacgtagctc ccccccggaa cgtccccaag
     3301 catcgacgcc tccagcgagc cagcctccaa ggtgttcgta gcccacgagg agcgattcgt
     3361 cgaacacgtc ctccaacggc gcatcgtcga gcacgaacag gagctgtgct tcgggcggca
     3421 ccagccatcc gagccacgtg gcgtaggtat cgccgaacgc gatgtggatg gcgttgggga
     3481 tgtgcccttc cgcgtactct tcctttgacc gcacgtcgat gatgagcgcg ccgccacgca
     3541 gggcgtgctc gaactcgtcg gccgagagcg gcagtgggag cggaatctcg cggcgcaatc
     3601 gaggaccc
//
LOCUS       DATMBB010000380         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_11889, whole genome shotgun sequence.
ACCESSION   DATMBB010000380 DATMBB010000000
VERSION     DATMBB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11889"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..633)
                     /locus_tag="VNL92_08110"
     CDS             complement(145..633)
                     /locus_tag="VNL92_08110"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HWO94713.1"
                     /translation="MANSSPATSADERTRPLALGTWVVNCDGASRGNPGEASIGCVIR
                     DPAGEIRRRVAKRLGWATNNEAEYEATIAGLQAARELGARHVELRLDSELIVRQLEGR
                     YRVRKAELRPRFERVMSLIREFDTFAVRHVRREQNREADALANLALDGFPPDAHDSAP
                     EG"
     gene            714..1742
                     /locus_tag="VNL92_08115"
     CDS             714..1742
                     /locus_tag="VNL92_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94714.1"
                     /translation="MTRLDRFAPWCVLAGIIIELTGLGIDGFLHRVDEGLASEELVSL
                     NVGHLLLGIGMALVAGGSALWLTAQLARRSSSWIVTIAPSIGILWLAIAAGAFALQAE
                     THDDTETVASNHAHTDEPGPSDVVRETDDHHGTDAPPLVSNSPPRGAGANAVHGSHAS
                     VPVSQEDLSLLNEQIAEAEEVALKYRDLRDALADGYVQTTQDLPGIAAHFVRPDLLAD
                     GEFDPAQPEMLLYSFRDGAWRFVGLSYAMPQAASAAEPEGFATSLDTWHYHNALCFRS
                     GAVVRAGGSSTQCEALGGRFSPKTPWMNHVWVGGYVPSDDLFGDRNPNLETGASMRIY
                     KPGDPVAS"
     gene            complement(1747..>2339)
                     /locus_tag="VNL92_08120"
     CDS             complement(1747..>2339)
                     /locus_tag="VNL92_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWO94715.1"
                     /translation="GNRMIGVSRRQRWTRRSDGSEQSAQYRYYQCESRTNQSRCDYHT
                     RRAEALEQQVRTALLEGDFVRPVLRAGDDEGVRAETEQLCEQLRSRAQALDKRLEALL
                     DAAVEGRLAPERLRSQCEALARDRQAIEDRLADALAQLDRHTSQREREARRRQDMDEI
                     ALRWESATPERRQEMLRRVLDRVVVHDERLEVHVKP"
BASE COUNT          376 a          833 c          714 g          416 t
ORIGIN      
        1 ccagtcgtcg tgatgtcgat gagccggtcg ttcgccaacg cctcccgaac ggtgtcgtcc
       61 attccccata cctccagcat cgcctgggcg ccggcttggt cgcgccggtc ctatcgtagc
      121 gcccgcaagc cctcgcctgg aagatcaacc ctccggagcc gagtcgtgag catccggcgg
      181 gaaaccatcg agggccaggt tagccagcgc gtcagcctcg cggttctgct cccgccgcac
      241 gtgacgcacg gcgaacgtat cgaactcgcg gatgagcgac atcacccgct cgaatcttgg
      301 ccgcagctcg gccttgcgga cacgatagcg accctcgagc tggcgaacaa tcagctccga
      361 atcgagacgg agctcaacgt gtctcgcccc caactcccgc gccgcctgaa gccctgcgat
      421 cgtcgcctcg tactccgcct cgttgttcgt ggcccacccc aggcgcttcg cgacccttcg
      481 tcgaatctcg ccggccggat cccgaatcac gcagccgatc gatgcctctc ctgggtttcc
      541 acgcgaggcg ccgtcacagt tcacaaccca cgtcccgagg gcgagagggc gagttcgctc
      601 atcggccgac gtcgcgggac tagaatttgc cacgccgaaa ccccgggaac ttgctcgaca
      661 tgcgataatc tccttcgtag atcgcctgtt tcgcaaggag cgcgaggatg agcatgacac
      721 gattggaccg gtttgcgcca tggtgcgtcc ttgccggcat catcatcgag ctcaccggct
      781 taggcatcga cgggttccta caccgagtcg acgagggtct cgcaagtgag gagctcgtca
      841 gcctcaacgt cggccatctg ctgctgggca tcggcatggc gctcgtcgcg ggaggctcgg
      901 cactttggct tacggcgcag ttggcccggc gttcttcctc gtggatcgtc accatcgcgc
      961 cctccatcgg catactttgg ctggccatcg cggccggcgc cttcgcgctt caggcggaga
     1021 cgcacgacga tacggagacc gtagcctcga accacgccca cacggacgag cctggcccca
     1081 gcgacgtcgt ccgcgagacg gacgatcacc atggcaccga cgctccgcca ctcgtctcga
     1141 actcccctcc gagaggtgcc ggcgcgaacg ccgttcacgg atctcatgca tcggtcccgg
     1201 tttcgcagga ggacctatct ctcctgaatg aacagatcgc ggaggcggaa gaggttgccc
     1261 tcaagtaccg tgacttgcgc gacgcgctcg ccgacggcta cgtccagacc acccaggacc
     1321 tgcccggcat cgccgcgcac ttcgtacggc cggatctgct cgcggatggc gaattcgacc
     1381 cggcgcaacc ggagatgctg ctgtactcct tccgcgacgg cgcttggcga ttcgtcggcc
     1441 tcagctacgc gatgccgcaa gcggcttcgg ccgccgaacc ggaaggtttc gccacgagcc
     1501 tggacacctg gcactaccac aacgcgctct gcttccgaag cggagccgtg gtgcgggcgg
     1561 ggggctcgtc cacacagtgc gaggccctcg ggggaaggtt ctcgccgaag acgccgtgga
     1621 tgaaccacgt ttgggtgggc ggctacgtgc cgagcgacga tctctttggc gatcggaacc
     1681 ccaacctcga aacgggggcc tccatgcgca tctacaagcc cggcgacccc gtcgcgtcgt
     1741 gatgcctcat ggcttaacgt gaacctcgag ccgctcgtcg tgtaccacca cgcgatcgag
     1801 cacgcgacga agcatctctt gccggcgttc cggagttgcc gattcccaac gcaaggcgat
     1861 ctcgtccatg tcctggcggc gccgcgcctc gcgttcccgc tggctcgtgt gccggtcgag
     1921 ctgtgctagc gcatccgcga gcctgtcctc gatcgcctgc cggtcgcgcg caagcgcctc
     1981 acactgcgat cgcaggcgtt ccggggcaag acgtccttcc acggccgcat caagcagcgc
     2041 ctcgaggcgc ttgtcgagcg cctgcgctcg cgaacggagc tgctcgcaaa gctgctccgt
     2101 ctctgcgcgc acaccttcgt cgtcgcctgc ccgcagcacg ggccgcacga agtcgccctc
     2161 gaggagggca gtgcgcacct gctgttcgag agcctccgct cgtcgcgtgt gatagtcgca
     2221 gcggctctgg ttcgtccggc tctcgcactg gtagtagcga tactgcgccg attgctcact
     2281 gccatccgag cgtcgggtcc atcgttgccg gcggctcacg ccgatcatgc ggttgccgc
//
LOCUS       DATMBB010000381         2604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_508946, whole genome shotgun sequence.
ACCESSION   DATMBB010000381 DATMBB010000000
VERSION     DATMBB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508946"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VNL92_08125"
     CDS             <1..1821
                     /locus_tag="VNL92_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HWO94716.1"
                     /translation="MVAQNSLVERIVEAVREERVQDVADVRNESGREAATRIVIELKK
                     GADPRVAEKQLLEFTPLRETVSIINIALVNRRPQTLGLHEMIDHYLSHRTDVIRRRTS
                     HLLAEARKKAHLLEGLLLAVCDIDEVIREIRASRSREEAIERLARRRFVIPSTHQHLS
                     KLPADLAARIARGGAEGLLLTRVQAESIGAMRLIQLVGLEIDRLVGDYAKVMGEIAEY
                     EGILANPRRVLELIRADLLEMRGRFDSPRLTTIEDAGEEIVVEDLIPVEDVAITISHA
                     GYVKRVPLETYRQQGRGGKGIRAGDARENDFIEHVFVAGTHDHLLCFTTTGRVFKIRV
                     YEIPEMSRTAIGRNIVNLIDLKEGERTCAYLAIKSFEAGSKFLTFVSRGGIVKRTALK
                     AYANVSRAGLIAVGLKEGDSLLDVAITDGQDDLLLVTAGGMAIRFNEQDARDMGRAAA
                     GVKGIELAEGDAVIGLAAIPMKPDADGDLMTADTSAALLTITERGYGKRTAIDEYRVQ
                     PESGKMRSQSRGGKGRVDIDTGERNGPSVAALIVRPTDDVVVVTRQGQLVRVGAEEIR
                     LCGRGSMGVRVVSLNEGDAVVAAACVPESERAETSDTGGR"
     gene            1821..2183
                     /locus_tag="VNL92_08130"
     CDS             1821..2183
                     /locus_tag="VNL92_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWO94717.1"
                     /translation="MPTDWSDDIVVSNLTDEPALSDELQAIVERVQFTRGRTPSIVLD
                     FAQVTYLNSSNLAQLLRLRQLLQERDADMRLCSLCDEIRSIFMVTGLERVFRFAPDAL
                     TALAGLQLERQSGKGQRP"
     gene            2180..>2604
                     /locus_tag="VNL92_08135"
     CDS             2180..>2604
                     /locus_tag="VNL92_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWO94718.1"
                     /translation="MTSGTAIQPSNGHASDAMTPVARLVGVRKTYFKPDGSVMVEALR
                     GVDVVIDRGEYVAIMGASGSGKSTLMNVLGCLDRATEGRYFLDGLDVGEMDDETLSAY
                     RGRKIGFVFQAFNLIPELTIDENVAVPLFYQRIPRRQRL"
BASE COUNT          460 a          929 c          804 g          411 t
ORIGIN      
        1 atggtggccc agaacagtct cgtcgagcgg atcgtcgaag ccgtgcgcga ggaacgcgtg
       61 caggacgtcg ctgatgtgcg gaacgaatcc gggcgcgagg ccgccacgcg cattgtcatc
      121 gaactcaaga agggcgccga cccgcgtgtg gccgagaagc agttgctcga gttcacaccc
      181 ctcagggaaa cggtttcgat catcaacatt gcgctggtca accgcaggcc gcagaccctc
      241 ggcctgcatg agatgatcga tcattacctg tcgcatcgca ccgatgtcat ccgccggcgc
      301 acctcccacc tgcttgccga ggctcgaaag aaggcccatc ttctcgaagg cctcctcctc
      361 gccgtgtgtg acatcgacga ggtcatccgc gagatccgcg ccagccgctc tcgcgaggag
      421 gccatcgagc gtctggcccg gcgccgattc gtgatccctt ccacgcacca gcatctctcc
      481 aaactccccg ccgacctcgc ggcccgcatc gcacgcggcg gggccgaggg actgctcctc
      541 acccgtgtgc aggccgagtc catcggcgcg atgcgcctca tccagttggt cgggctggag
      601 atcgaccgcc tcgtcggcga ctacgcaaag gtcatgggcg agatcgctga gtacgaaggc
      661 atcctcgcca acccgaggcg cgtcctggaa ctcatccgtg ccgacctgct cgagatgcgc
      721 ggacgcttcg actcgccgcg ccttaccacc atcgaggacg ccggcgagga gatcgtcgtc
      781 gaggacctca tccccgtcga ggacgtcgcc atcaccatca gccacgccgg ctatgtcaag
      841 cgcgtgccgc tggagaccta ccgccagcag gggcgcggcg gcaagggcat ccgcgccggc
      901 gacgcacgcg agaacgactt catcgagcac gtcttcgtcg ccggcaccca tgatcatctt
      961 ctgtgtttca cgacgacggg gcgcgtcttc aagattcgcg tctacgagat tcccgagatg
     1021 tctcgcaccg ccatcgggcg caacatcgtc aacctcatcg acctcaagga gggcgagcgc
     1081 acctgcgcct acctcgcgat caagagtttc gaggccggca gcaagttcct caccttcgtc
     1141 agccgtggcg gcatcgtcaa gcgcaccgca ctcaaggcct acgccaatgt cagccgcgcc
     1201 ggcctgatcg ccgtcggtct caaggagggc gattcgctgc tcgacgtcgc catcaccgat
     1261 gggcaggacg acctgctgct cgtcacggcc ggcggcatgg ccattcgctt caacgaacag
     1321 gacgctcgcg acatggggcg tgcggcggca ggcgtcaagg gcatcgaact cgccgagggc
     1381 gacgcggtca tcgggctggc cgccatcccc atgaagcccg acgccgacgg cgacctcatg
     1441 accgctgaca cgtccgccgc cctgctcacg atcaccgagc gcggctacgg caagcgcacg
     1501 gccatcgatg agtaccgcgt ccagccggag agcggcaaga tgcgctccca gtcccgtggc
     1561 ggcaaggggc gcgtggacat cgacaccggc gagcgcaacg ggcccagcgt cgcggccctc
     1621 atcgtgcgtc ccaccgacga cgtggtggtc gtcacgcgcc agggacaact cgtgcgcgtc
     1681 ggcgcggagg agatccgcct ctgcggacgc ggctcgatgg gggtgcgcgt cgtgtccctc
     1741 aacgagggcg acgcggtcgt cgccgcggcg tgcgtgcccg agagcgaacg cgccgagaca
     1801 tcggacaccg gaggccggtg atgcccacgg actggagcga tgacatcgtg gtctcgaacc
     1861 tgaccgacga gcccgcgctc tcggacgagt tgcaggccat cgtcgagcga gtccagttca
     1921 cgcgcgggcg cacgcccagc atcgtgctcg acttcgccca ggtgacgtac ctcaacagtt
     1981 ccaaccttgc ccaactcctc cgcctccgcc aactcctgca ggaacgtgac gccgacatgc
     2041 gcctctgcag cctctgcgac gagatccgtt cgatcttcat ggtcacgggg ctcgaacgcg
     2101 tgtttcgttt cgccccggat gccctgacgg cgctcgccgg ccttcagctc gagcggcaga
     2161 gcggcaaggg gcagcgccca tgacgtccgg cacggccatc caaccctcca acggacacgc
     2221 gtccgacgcc atgacccccg tggcacgcct tgtcggggtc cgcaagacct acttcaagcc
     2281 cgacgggtcc gtcatggtcg aggcgctgcg cggagtggac gtcgtcatcg atcgcggcga
     2341 gtacgtcgcc atcatgggcg cctccggctc gggcaagtcc acgctcatga acgtcctcgg
     2401 atgcctcgac agggccaccg aaggccgcta cttcctcgat gggctcgacg tcggcgagat
     2461 ggacgatgaa accctcagcg cctatcgcgg acgcaaaatc ggcttcgtgt tccaggcctt
     2521 caatctcatc cccgaactta ccatcgacga gaacgtcgct gtgcctctct tctatcagcg
     2581 cattccgcgc cgccaacgcc tcga
//
LOCUS       DATMBB010000382         3833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_249814, whole genome shotgun sequence.
ACCESSION   DATMBB010000382 DATMBB010000000
VERSION     DATMBB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249814"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VNL92_08140"
     CDS             complement(<1..1293)
                     /locus_tag="VNL92_08140"
                     /inference="COORDINATES: protein motif:HMM:NF015149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HWO94719.1"
                     /translation="MSIRLRPAALLLLAMAALAPLALPTGSTAQVPVTDFTFADLGIE
                     DIRSTGIVSSQTVRFPGPGDVRLAPDGSYLALGVSHSELLDPELSTMTVTLNGFRLRS
                     FRLDATNAQGATLGIPVHASQFRRGTNELRVDFYLRMRQCEAGPEDPSRYAIVDNASA
                     VHYAYATDEPKFALQDFSLSTFPYPFYHSGYVEQPQTTVVVPREPSEDELSAAMDVAV
                     ALGRSIGQQAYEPQLVTVDAFDRQSMAAHHLILIGTPERNPLVAEFSAALPVRAEDNR
                     LVATDGSKLPAHFAALSLGRSPLNNERGLLLVTATDGAALPLASGELIRNAGDHPLTG
                     PYALYEWDRADDQARNDPEPVDGSLRLTFGELGIDETTVSGFGPNIVTVPFYAPRPIP
                     GSASTLRLQLSHANVLDLADSALRVELNGEVVATELLPA"
     gene            complement(1451..3004)
                     /gene="rpoD"
                     /locus_tag="VNL92_08145"
     CDS             complement(1451..3004)
                     /gene="rpoD"
                     /locus_tag="VNL92_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWO94720.1"
                     /translation="MVTGFDDTQAVTELIEKGRRQGHVTAEDILEILPKPEEDLDRLE
                     EIQTQLLDNSIELIDADQLLPSITGDRDTSDEEPEPERVPTPSEAEEEEGTDDPVRMY
                     LREIGKVYLLSAADEKRLARQMEDGNYIEAIEAALQDETGRAPSAAQVGARLYNHLAE
                     LKPVLDRAISWLKLDASMPLSELVADESFRGLVDGEADEEFQTHVSEALAIEPEDAHQ
                     RIVRLSIVTHILTPQLVSRAEEVLQPGESLLPPPSDLAERLIPHEGRYEYHFERLKDE
                     GRRAERQLTEANLRLVVSVAKKYIGRGMSLLDLIQEGNIGLIRAVEKFDYRKGFKFST
                     YATWWIRQAITRAIADQARTIRIPVHMVETINKLVRISRRLVQEYGREPTSEEIGRGM
                     EISPEKVREIIKVSQEPVSLETPIGEEEDSHLGDFLPDETALAPADAASHQLLKEQVM
                     EVLASLTPRERKVLELRFGLEDGRSRTLEEVGREFRVTRERIRQIEAKALRKLRHPSR
                     SKKLKDYLE"
     gene            complement(3009..>3833)
                     /locus_tag="VNL92_08150"
     CDS             complement(3009..>3833)
                     /locus_tag="VNL92_08150"
                     /inference="COORDINATES: protein motif:HMM:NF021895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94721.1"
                     /translation="LVAEARPVLDHLMDVARERYELTQPRERSRAVDEILPVLAGIQD
                     QVVLMDYLQRLATLARVELPVLSERLRAAQRKRAPRTQGEPAGQARAAIPQKKGAREE
                     FILALLHRHPSLRARGLDLSPAMFWDSQHRALFEAWRDERDTAADEHLRAPLERILQR
                     ELPPYDAQQADEALASAIRLLERERLLEAKNAAASALAEETRRIGEETLVDSAVRVLA
                     GRGAAQDDDLAPAVAVAIADVDAGLRLHGQAARGTNEPHEKAVGSVEDECIEMTTE"
BASE COUNT          587 a         1218 c         1319 g          709 t
ORIGIN      
        1 ggcgggcagc agctccgtgg cgacgacctc gccgttcaac tcgacgcgga gcgccgaatc
       61 cgcgaggtcg aggacgttcg catgcgagag ctgcagtcta agtgtcgacg ccgacccggg
      121 gatcggccgc ggcgcgtaga agggcaccgt aacgatgttc ggcccgaaac cgctgaccgt
      181 cgtttcgtcg atgccaagct ctccgaacgt caggcgcagc gagccgtcga ctggctcggg
      241 gtcattccgt gcttggtcgt cggctcggtc ccactcgtag agggcgtacg gcccggttag
      301 ggggtggtct ccggcgttgc gaatcagctc tccgctcgct agggggagcg cggcgccatc
      361 ggtggcggtt accagcagca gaccgcgctc gttgttcagc ggcgacctgc cgagagagag
      421 cgccgcgaag tgagcgggga gtttgcttcc gtctgtcgcg acgaggcgat tgtcctcggc
      481 gcggaccgga agggcggcgc tgaactcggc gacgagcggg tttcgctcgg gggtcccgat
      541 gaggatgagg tggtgtgcgg ccatgctctg ccggtcgaag gcgtccacgg tgacgagttg
      601 cggctcgtat gcctgctggc caatcgagcg gccgagcgcc acggcaacat ccatcgcggc
      661 gctcagctcg tcttcactcg gctcgcgcgg cactacgacc gtcgtttggg gctgctcgac
      721 ataacctgag tgatagaagg gatacgggaa cgtggagagc gagaagtcct gcagggcgaa
      781 cttgggctca tcggtggcgt aggcgtaatg caccgcggac gcgttatcga cgatggcgta
      841 gcggctcggg tcctccggtc ccgcttcgca ctggcgcatg cggaggtaga agtcgacgcg
      901 gagctcgttc gtgcccctgc ggaactgcga ggcgtgcact gggatgccaa gcgtcgcgcc
      961 ctgggcgttg gtcgcatcga gacggaaaga gcgcaggcgg aagccgttca gggtcaccgt
     1021 catcgtggag agctcggggt cgagcaactc cgagtgcgag acgccgagcg ccagatagga
     1081 gccatcgggc gcgaggcgca catcgccggg gccggggaag cgcacggtct ggctggaaac
     1141 gatgcccgtc gagcggatgt cttcgatgcc gaggtcggcg aaggtaaagt cggtcacggg
     1201 cacctgcgcc gtcgaacccg tgggtagagc cagcggggcg agcgctgcca tggcgaggag
     1261 aagtaaggcg gcggggcgca agcgaatgct caacttcgcg tccggatact gttcatgctt
     1321 gtactagcgt aggggtgttg gggtatcgag tgagttacgg tgcgtcaccg atgcgtcaag
     1381 atgcggtcac cgaaaagcgt agggcgcacc cactaggagt acgccctacg tcaacgttcg
     1441 ttggcgaccg ctactcgagg tagtccttga gcttcttcga tcgggaggga tggcgcagct
     1501 tgcggagggc cttcgcttcg atctgccgaa tccgctcgcg cgtcacgcgg aactcgcggc
     1561 cgacctcctc gagtgttcgg cttcggccat cctccaggcc gaaacgaagc tcgagcactt
     1621 tgcgctcgcg cggcgtaagc gaggcgagca cctccatcac ctgttccttc agcagctgat
     1681 ggctggcggc atcggccggc gccagcgccg tctcgtccgg aaggaagtcg ccgaggtgac
     1741 tgtcctcctc ctcgccgatc ggtgtctcga gggagacggg ctcctgggag accttgatga
     1801 tctcccgcac cttctccggc gagatttcca tgccccgacc gatctcttcg ctcgttggct
     1861 cgcgcccata ctcctgcacg aggcggcggc taatgcgaac gagcttgttg atcgtctcca
     1921 ccatgtgcac cgggatgcgg atggtccgcg cctgatcggc aatggcccgc gtgatcgctt
     1981 ggcggatcca ccacgtcgcg tacgtgctga acttgaagcc cttgcggtag tcgaacttct
     2041 cgaccgcacg gatgaggccg atgttgcctt cctgaatgag gtcgagcaac gacatcccgc
     2101 gcccgatgta cttcttcgct acgctgacga cgaggcgaag gttcgcttcc gtcagctgac
     2161 gttcggcgcg ccgtccctcg tccttcagcc gctcgaagtg atactcgtag cggccttcgt
     2221 gtgggatgag ccgctcggcc aggtcgctgg gcggcggcag aagcgactcg ccgggctgga
     2281 gtacttcctc cgctcgtgag acgagttgcg gcgtgaggat gtgcgtgacg atggacagtc
     2341 gcacaatgcg ttgatgcgca tcctcgggct cgatggcgag cgcctcgctc acgtgcgtct
     2401 ggaactcttc gtccgcttcg ccatcgacga ggccgcggaa cgactcgtcg gcgacgagct
     2461 cggaaagtgg catcgaggca tcgagcttga gccaggagat cgcccgatcg agcaccggct
     2521 tcaactcggc gaggtggttg tacagccgcg ctcccacctg ggcggcactc ggggcgcgtc
     2581 ctgtctcgtc ctgaagcgct gcctcgatcg cctcgatgta gttgccatcc tccatctgcc
     2641 gcgcgagccg cttctcatcg gccgccgaga ggaggtagac cttgccgatc tcccggaggt
     2701 acatgcggac cgggtcgtcg gtgccctctt cctcctcggc ttcactcggc gtgggaacgc
     2761 gctcgggctc gggctcctcg tcggacgtgt cccggtcgcc agtgatcgac ggcagaagtt
     2821 ggtcggcgtc gatgagctcg atgctgttgt cgagcagctg agtctggatc tcctcgaggc
     2881 gatccaggtc ttcctccggt ttcgggagga tctcgagaat gtcctcggcg gtcacgtggc
     2941 cctgccggcg tcccttctcg atgagttccg ttactgcttg cgtatcgtca aaaccagtta
     3001 ccatgtcgtc actccgttgt catctcaata cattcgtcct caaccgaacc tacggccttc
     3061 tcgtgcggtt cgttcgtgcc cctcgcggcc tgcccgtgca gccgaaggcc cgcatcgaca
     3121 tccgctatcg caacggcaac cgcgggggcg aggtcgtcgt cctgggcggc cccgcgtccg
     3181 gcgagcacgc gcacggccga atcgacgagg gtctcctccc caatgcggcg cgtctcttcg
     3241 gccaaggccg atgcggcggc gttcttcgcc tccagcagtc gctcgcgctc cagcaaccgg
     3301 atggcgctgg ccagcgcttc gtccgcttgc tgcgcgtcgt acggcggcag ctcgcgctga
     3361 aggatgcgct cgagcggcgc cctcaagtgt tcgtcggctg ccgtgtcgcg ctcgtcacgc
     3421 caggcctcga atagggcccg atgctggctg tcccagaaca tcgcgggcga gagatcgagg
     3481 ccccgggctc gcagcgaagg atgccgatgg aggagggcga ggatgaactc ctcgcgggcg
     3541 cccttcttct gcgggatagc cgcgcgggct tggcccgcag gctctccctg ggtccgtggc
     3601 gcgcgcttac gttgggccgc cctcagccgc tcgctgagca ccggcagctc gactcgggcg
     3661 agcgtcgcga gacgctgcag gtagtccatc agcaccacct ggtcctggat gccggcaagg
     3721 accggcagga tttcgtcgac ggcgcgcgac cgttcgcgcg gttgggtcag ctcgtaccgt
     3781 tcccgcgcca cgtccatgag gtggtcgagc acgggccggg cctccgcgac gag
//
LOCUS       DATMBB010000383         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_300698, whole genome shotgun sequence.
ACCESSION   DATMBB010000383 DATMBB010000000
VERSION     DATMBB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300698"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2677)
                     /gene="cas3u"
                     /locus_tag="VNL92_08155"
     CDS             complement(<1..2677)
                     /gene="cas3u"
                     /locus_tag="VNL92_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HWO94722.1"
                     /translation="MADLTPDDFAAFFEAVHSTDKETRKPFNWQARLAKEVLAARRWP
                     DVIRVPTGCGKTGVLDLALFDLALQAPLRAEDRTAARRICFVIDRRLVVDEVTEHAQR
                     ILRAILRGVEGSGVAPVVKAVAQQLAGLAADSAQPLRLVRLRGGVYRDDGWAADPLTP
                     TILVSTVDQVGSRLLFRGYGVSRRNLPVHAGLLAFDTRIILDEAHLSAVFADTLDSIR
                     RFQKLAEAPPLSEGRLLTVTRMSATVGGGGTTFELSGDDHNDVRLRARLLASKPARLV
                     VVNVEPVTKKLRKEQPRKARAQEQKNRKALVEEIVRQVQCHAGFGAGNGQPDSTRVIG
                     VVANRVATARRVFEKLRELENETGSKALLLTGRIRPFDRDRLLDEWLPKIRAGREQEP
                     EDRLFVVATQTVEVGANLDFDALVTEAASLDALRQRFGRLDRLGNRHERGAPSPASIV
                     IRSDQAKSSDDDPIYGSAVAKTWHWLSSNASTTGRGAVKQRRVDFGINSLDAKLPKDP
                     DVLKPMLAEIAETPVLFPAHLDAWAQTNPRPDPDPDVGPFLHGRSDTPPDVLVVWRAD
                     LNDENLEHWPLIVSLMPPLTREALPVPIYEVRAWLRRAAEGDVTDLEGTEAESNDSSA
                     APPGEERRVVRWRGKDDARVVGPDEIRPGDTVVVPARYGGSDRFGWDPGQKNAVEDVA
                     ERCLAQLIASYPPNAFRRPKLRFRMHPSLLPQADEDTRYRLNVLLQRVVDAAGSEGQD
                     AWEATRHLLDSFLPLVSDPHIETAIKALQKALQGRRCRLMPYPASNGVVAVASVKLDM
                     SQTLPEVEQEEEEPEGDEASFTGHQVPLDEHLKNVSKTAHDFASRSGLAGALIKTVEL
                     AGRWHDQGKRDWRFQAWLRGSELEALAEDIPIAKSG"
     gene            complement(2681..>3510)
                     /gene="csb2"
                     /locus_tag="VNL92_08160"
     CDS             complement(2681..>3510)
                     /gene="csb2"
                     /locus_tag="VNL92_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HWO94723.1"
                     /translation="FGRLWVFQPDKDDPALPIVATLRVTQALRRALLRNVHDAVCGCK
                     RWRGQVPSCREASKCYAKIPGMLSSHAPDCGPLKAPHVAFAPLPFVHPVQRHADGEIK
                     GVGILVPRDMERDLSVMAMLARGLRGIEQHGLRIPGVGEWRLREVSADDPPLMTLDPL
                     TWTEPSHAWATVTPMVFGHFPKPDKEGEAKVVLDSLHLAGIDPSRVVEIAVGQHSPLH
                     GVPPSWSFNTRRDRNEAEEPRRLVRHVTIRFDAPVEGPLALGALRYFGLGLMRPLAA"
BASE COUNT          607 a         1165 c         1110 g          628 t
ORIGIN      
        1 gaccagactt cgcgattgga atgtcctcag caagggcctc taactcggaa ccgcgcagcc
       61 atgcttggaa tctccagtcg cgcttaccct ggtcgtgcca tcggccggcc agctcgacgg
      121 tcttaatgag cgcgccggcg aggccacttc tgctcgcgaa gtcgtgggcg gttttactga
      181 cattttttaa gtgctcatca agcgggactt ggtgcccggt gaaagatgcc tcgtctccct
      241 cgggctcctc ttcctcttgc tcgacctcgg gcaatgtctg cgacatgtca agcttcaccg
      301 aggccactgc cacaactccg ttggaggccg gatacggcat aagtcggcat cggcgcccct
      361 gcaaagcctt ctgcaaagcc ttgattgcgg tctcaatgtg cggatcgctc accagcggca
      421 aaaacgagtc gagtaggtgg cgcgtcgcct cccaggcgtc ctgtccctcg gaacccgccg
      481 cgtcgaccac cctttgcaga agcacgttta gacggtagcg ggtgtcttcg tccgcttgcg
      541 gcagcaggga ggggtgcatg cggaatctca gcttgggccg acgaaaggca ttgggaggat
      601 aggatgcgat cagctgggcg aggcatcgct ctgcgacgtc ctccaccgcg ttcttctgac
      661 cagggtccca accgaatcgg tcgctacctc cgtagcgcgc cggaaccacg accgtatcgc
      721 ctggccgtat ttcgtcgggc ccgaccactc gggcgtcatc cttgcctcgc caccgcacga
      781 cgcgccgctc ctcgcccggc ggcgcagcgg atgaatcatt cgactcggct tccgttcctt
      841 ccaggtcagt tacgtcccct tcggccgctc tccgcagcca cgcccgcacc tcgtagattg
      901 gaacaggcag agcctcgcgg gtgagcggcg gcatcagcga gacaatcaga ggccaatgct
      961 ccaggttctc atcgttcagg tcggcgcgcc acacgaccag gacatccgga ggcgtgtcgg
     1021 agcggccgtg gaggaacggc cccacgtcgg gatccggatc cgggcgcggg ttggtctggg
     1081 cccacgcatc gaggtgcgca ggaaacagca cgggagtctc tgcgatctca gcgagcatcg
     1141 gcttcaagac atcaggatcc ttgggcagct tcgcgtccag cgagttgata ccgaagtcga
     1201 ccctccgttg cttcacggca cccctgcccg tcgttgaagc attcgaggaa agccaatgcc
     1261 aggttttagc gaccgccgag ccgtagatgg gatcgtcgtc gctgctcttc gcttgatcac
     1321 tgcggatgac gatactcgcc ggcgacggtg cgcctctttc gtgtcgattc ccaagccggt
     1381 caagccggcc gaacctctgt cggagggcat ctagcgatgc tgcttccgtg accagcgcgt
     1441 caaaatcgag gttggccccg acctcgacag tttgcgtcgc aaccacgaag agcctgtctt
     1501 ctggctcttg ctcccgacca gcgcggattt tgggcaacca ctcgtcgagg agacggtctc
     1561 ggtcgaaggg tctgatgcgt ccggtgagca gtagcgcctt cgagccggtc tcgttttcga
     1621 gttcacggag tttttcgaac acgcgccgcg ccgtggcgac gcggttcgcg acaacgccga
     1681 tcactcgcgt cgagtcgggc tggccatttc ccgcgccgaa tccggcgtgg cactgcacct
     1741 gcctgacgat ctcctcgacg agagccttgc ggttcttctg ctcctgcgca cgtgccttgc
     1801 gaggttgctc cttacgaagt ttcttggtga ctggctccac gttcaccacg accaagcgcg
     1861 ccggcttcga cgccaggagc cgggccctca ggcgcacgtc gttgtggtcg tctcccgaga
     1921 gctcgaacgt cgtgccacct ccaccgacag tcgctgacat ccgagtgacc gtgaggagac
     1981 ggccttcgga caacggcggg gcctcggcga gcttctggaa tcgccggatg gagtccaggg
     2041 tgtcggcgaa gacggcggac aggtgcgcct cgtcgaggat aatgcgtgtg tcgaaggcga
     2101 gcagcccagc atggaccggc aggttgcgcc ggctgactcc gtagccgcgg aacaacaagc
     2161 gcgagccgac ttggtcgacc gtcgagacaa ggatggtggg cgtaagcggg tccgcagccc
     2221 acccgtcgtc gcgatacacg ccgccgcgca accgcacaag cctcagcggc tgggcgctgt
     2281 ctgccgccag cccggcgagc tgctgagcga cagctttcac cacgggcgcg acgccagaac
     2341 cctctacacc tctcaggatc gcgcggagga tgcgctgggc gtgctccgtc acctcgtcga
     2401 cgacgagccg ccggtcgatg acgaagcaga tcctccgtgc agccgtccgg tcttctgccc
     2461 tcaggggcgc ctgaagagcc aagtcgaaca gcgccaggtc cagcacgccc gtcttgccgc
     2521 agccggttgg cacgcggatc acgtcgggcc agcggcgggc ggcgagcacc tccttggcca
     2581 gacgcgcctg ccaattgaaa ggtttcctcg tctccttgtc cgtgctgtgc acagcctcga
     2641 agaacgcggc gaagtcatcg ggcgtgagat ccgccatcgc ctacgccgcc aacggccgca
     2701 tcaagccgag accgaagtag cggagcgccc caagcgcgag aggcccttcg accggcgcgt
     2761 cgaaccggat cgtcacgtgc cggacgagcc ggcggggctc ctcggcctca ttccgatcgc
     2821 ggcgcgtatt gaaggaccaa ctcggtggga cgccatggag tggcgaatgt tggcccaccg
     2881 cgatctcgac gacccgtgag ggatcgatcc cggcgaggtg cagcgaatcg agcaccacct
     2941 tcgcttcgcc ctctttatcc ggcttcggga agtggccgaa aaccatcggt gtcaccgtgg
     3001 cccaggcgtg agagggttct gtccatgtga gtggatcgag cgtcatgaga ggcggatcat
     3061 cggccgacac ttcccgtagc cgccactcac ccacgccagg gatccgcagt ccatgctgct
     3121 cgatgccgcg cagccctcgc gccagcatcg ccatcacaga caggtcgcgc tccatgtcgc
     3181 gcggcacgag aatgcccacg cccttgatct cgccgtcggc gtgccgctgt acaggatgaa
     3241 cgaagggcag cggcgcgaac gccacgtgcg gagctttgag gggaccgcag tctggcgcgt
     3301 ggctgctcag catgcccgga atcttcgcgt agcatttgct ggcctcccga cacgacggaa
     3361 cctggcccct ccagcgttta cagccgcata cggcatcgtg gacgttcctg aggagggcac
     3421 gacgcagcgc ctgggtgacc ctaagcgtcg cgacgatggg cagtgcgggg tcatccttgt
     3481 caggctggaa aacccacagg cggccgaaga
//
LOCUS       DATMBB010000384         4532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_219817, whole genome shotgun sequence.
ACCESSION   DATMBB010000384 DATMBB010000000
VERSION     DATMBB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219817"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VNL92_08165"
     CDS             complement(<1..663)
                     /locus_tag="VNL92_08165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWO94724.1"
                     /translation="MRDDVEATPEERVMSLAYKVSAYNRQRKFEAFMEMVAPNETTKI
                     LDVGFSDDEYSDSDNFLEKHYPYPENITALGLDVPARFSHRYPRVSAVQYDGKRFPFK
                     DKAFDVCWSNAVLEHVGSRQDQILFLREVARVADLGWVTTPNKHFPVEVHTRTPLLHY
                     LPKPVFHKYLCVTGRQWAAGDYMNLLSLGEARKLLAAANIKSYSIFKNRLLAFPLDFV
                     IVL"
     gene            1150..1659
                     /locus_tag="VNL92_08170"
     CDS             1150..1659
                     /locus_tag="VNL92_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWO94725.1"
                     /translation="MTERTGHVVQINISQGGVPKLPVPRARVGFTGLEGDGHSDKRNH
                     GGPDRAVSLLAIEVIDQLRAEGHPIAPGSTGENLTVSGLEYTDLQRGDRLQIGNEVLL
                     ELTTNATPCTTIADSFQDGDYSRLSHKRHPGSARWYARVLRAGFIETGAEIRLLQSEG
                     IPQGGAASV"
     gene            1656..2483
                     /locus_tag="VNL92_08175"
     CDS             1656..2483
                     /locus_tag="VNL92_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWO94726.1"
                     /translation="MTHHREALGALMRTPPAQIDLAYAALLIAQEAYPNLDPNPYLAQ
                     LDSMAAELRSRLRASAGAPKTLQAMNRYLFEEIGFRGNTEEYYDPRNSYLNDVLDRRV
                     GIPITVSLIYMEVARRLGIELQGVAMPGHFLVKYARREGQVVIDPFHTGAVLSAKDLR
                     ERILAAGGKPEEASLYLAAATKRQIITRMLHNLRHIHLAGRDFDRALGIVEMMLAVSP
                     WDLDAIRDRGLIQYQRHAYSEALRDLEVYLEYRPEADDAGSIWESVQTLRPRVSGQE"
     gene            2495..3229
                     /locus_tag="VNL92_08180"
     CDS             2495..3229
                     /locus_tag="VNL92_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWO94727.1"
                     /translation="MGELAGHDGERNRPAARGVPFIGIAHGAGNDRVLLNRAVEGGVD
                     YVEADLWPRGSNDVVARHERRFPFLPLLYDKWYIKPDVRPVYLEEIVAAAAAKGVRVY
                     ADIKSGRYGFVDRVISILRRGGAYEGAILSGHHWFDLARAKRELGIAAFPSIPDGTAL
                     DRFWSFIGRRAEMFDGVAIRHWLITPRTVSRFRDLGLRVLPWTVDSHSRAHELMALGA
                     DGVISNSVDLIHEMSLAQVSALPRAG"
     gene            complement(3204..4316)
                     /locus_tag="VNL92_08185"
     CDS             complement(3204..4316)
                     /locus_tag="VNL92_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWO94728.1"
                     /translation="MTGELDDRPAKLELRGVRLRRGRFTLDVPSLALREGEVLAVMGP
                     NGSGKSTLLHTAALLERPQAGELRFDGVSVRGSERKYRRRMAVVFQEPLLLDRSVRAN
                     VSLGLRLRGLRERRAREDRWLEAFGVLHLADRVARSLSGGEAQRVNLARAFALQPEVL
                     LLDEPFASLDAPSRESLADLLSRLIRETGVTALFVTHDRGEALRFGDRIAVLIDGRVR
                     QVGPAQEVFTAPSDAEIAAFVGIETILSGRVTEHSDGVVQVQVGPAEAGVLIDAAVGS
                     EIARDVLVCVRPEDVTIGEVQEGTRTSARNQFIGKVVRVATSGLQVRVELDCGVPLVA
                     LITQRSARELDVREGAPYLVAFKASAVHLIPRAEGR"
     gene            complement(4313..>4532)
                     /locus_tag="VNL92_08190"
     CDS             complement(4313..>4532)
                     /locus_tag="VNL92_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tungstate transporter permease"
                     /protein_id="HWO94729.1"
                     /translation="MAGFGAVISEVGASMQVGGNISGETRVLTTAAVLETQKGNFELA
                     LALGIILLAIMFTIVILLTVLQQQRRSL"
BASE COUNT          855 a         1478 c         1416 g          783 t
ORIGIN      
        1 caggacgatc acaaagtcca gaggaaaggc gagtagtcgg tttttgaaga tcgagtagga
       61 cttgatgttg gcagccgcga gtaacttgcg tgcttcccca agcgacagca gattcatgta
      121 gtcaccggca gcccattgcc ttccggtaac gcagaggtac ttgtggaaca ctggctttgg
      181 cagatagtgt agcaggggcg tgcgggtgtg tacttcgacc ggaaagtgtt tgttgggcgt
      241 cgtaacccaa ccaaggtccg caactctggc tacttcccgc agaaacaaga tctgatcctg
      301 tctgctaccc acatgttcga gcactgcatt ggaccagcag acatcgaagg ccttgtcctt
      361 gaacgggaag cgcttaccgt cgtactggac cgctgagacc cgaggatacc tgtgagagaa
      421 tcgagccgga acatcgagac caagggcggt aatgttttca ggatacgggt agtgcttctc
      481 caaaaagttg tccgaatcgc tgtactcatc gtcgctgaaa ccaacatcca ggatcttcgt
      541 cgtctcgttg ggcgcaacca tctccatgaa agcttcgaat tttcgctgcc tgttgtacgc
      601 ggacaccttg taggccaacg acatcacgcg ttcctcgggt gttgcctcaa cgtcgtccct
      661 cacccgtgcg cattggcatg ttcgggactg ctgaactgcg accgtggccg cgtttgtcgg
      721 aaccttgctc tttcgcctcg cggaaccgag cggcctgctt cgacactaat ggacatggca
      781 ggcacggcgc gtaagggcgt ggtttgagtt gggtctgatt tcagtcagct gtagccggcg
      841 ctagcgtacg cgagcgggcg aatcatcatt ccgtgcggca caaccctgtc cgtaccgcca
      901 ccgcctccga gtgaaagcac cgtcatggca cgactctcgg gcatgagaag gcggcggagg
      961 ggcggaggca cgccgataac gtctcgtggc caggacagag cgtcagcgag atccagactc
     1021 gggagcatcc gccgagcaca tgtccgtcca ctccggccac gtccagacgc cgatcagggc
     1081 gaacgagact tcaacctaag ctgccataac gcgtatactg gcaaccgaag agtgtggcgg
     1141 gagggggtct tgaccgagcg cacaggccac gttgtacaga taaacatctc gcagggcggg
     1201 gtgccaaagc ttcctgtgcc gcgcgctcgt gttggattca ccggcctgga aggcgacggt
     1261 catagcgata agcgcaacca cggcgggccg gaccgcgccg tgtcgctcct cgccatcgag
     1321 gtcatcgatc agctgagggc ggaggggcat cccatcgctc ctggctccac aggcgaaaat
     1381 ctgaccgtct ccgggctgga atacaccgac ctacaacgcg gcgaccgcct ccagattggc
     1441 aacgaggtcc tgctcgagct gaccacaaac gcgacgccgt gcacgacgat cgccgattcg
     1501 ttccaagatg gcgactacag ccgcttgtcg cacaagcgac atccgggctc cgcgcgctgg
     1561 tacgcacgcg tgctgcgcgc cggcttcatc gagacaggcg ccgagatccg cctgcttcag
     1621 tccgagggga ttccccaagg aggcgcggcc tcagtatgac gcatcaccgc gaggctctcg
     1681 gcgccctgat gcgcacgccc cccgcccaga tcgacctcgc ttacgcggcg ctcctcatcg
     1741 ctcaggaggc gtaccctaac ctcgacccga acccctacct cgcgcaactc gactcgatgg
     1801 ccgccgaact acgctctcgg ctgcgtgcat ctgcgggagc gcccaagacg cttcaggcca
     1861 tgaaccgcta cctcttcgag gagatcggct ttcgaggaaa caccgaagag tactacgatc
     1921 cgcggaatag ctacttgaac gatgtactcg atcggcgcgt aggtatcccc atcaccgtga
     1981 gcctcatcta catggaagtt gcgcgccgcc tcggcatcga gttgcagggc gtcgccatgc
     2041 ccggccactt cctcgtcaag tacgcccggc gcgaggggca agtcgtcatc gatccctttc
     2101 acaccggcgc cgtcctctcc gcaaaggacc tgcgggaacg catactggcg gcaggcggaa
     2161 agccggagga agccagcctc tacctcgccg cggccaccaa gcgccagatc atcacccgca
     2221 tgctgcacaa cctgaggcat atccaccttg cgggccgcga tttcgaccgc gcgctcggaa
     2281 tcgtcgagat gatgcttgca gtcagtccgt gggacctgga cgcgattcgc gatcggggcc
     2341 tcatccagta tcagcgtcat gcctactcgg aggccctccg cgacctcgaa gtctacctcg
     2401 agtaccggcc ggaggcggac gacgctggga gcatctggga gagcgtgcag acattgcggc
     2461 ctcgagtttc gggacaggag tgagagcgga ggatatgggc gagcttgcag ggcatgatgg
     2521 cgagcggaat cgaccggcgg cgcggggcgt tcccttcatc ggcattgcgc acggcgcggg
     2581 caacgatcgc gtcctgctca atcgtgccgt agaaggaggc gtcgattacg ttgaggccga
     2641 cctgtggccg cgcggcagca acgatgtcgt ggcgcgacac gagcgccgtt tcccgttcct
     2701 ccctctcctg tacgacaagt ggtacatcaa gcccgatgta cgcccggtgt acctcgaaga
     2761 gatcgtcgcg gcggccgcgg ccaagggtgt acgggtctat gccgacatca agagcggccg
     2821 ctatggcttt gtcgaccgcg tcatctctat cctgcggcgc ggcggcgcgt acgagggcgc
     2881 catcctcagt gggcatcatt ggttcgacct cgccagagcc aaacgagagc tgggcatcgc
     2941 tgccttcccc tcgatccctg acgggacggc gctcgaccga ttctggtcgt tcatagggcg
     3001 ccgagcggag atgttcgacg gcgtcgccat tcgtcattgg ctgatcacgc cgcggaccgt
     3061 gagccgcttc cgcgatctcg ggttgcgcgt gcttccgtgg accgtcgatt ctcacagccg
     3121 ggcgcacgag ctgatggcgc tgggggccga cggcgtcatc agcaacagcg tcgacctcat
     3181 ccatgagatg tcgctggcgc aggttagcgc ccttccgcgc gcgggatgag gtgcacggcg
     3241 ctagccttga aggcaacgag gtatggagct ccctcgcgaa cgtccagctc acgggcggac
     3301 cgctgcgtga tgagcgcgac caggggcacg ccgcagtcca attccacgcg tacctgcagc
     3361 cccgacgtgg ccactcgcac gaccttgccg atgaactgat tgcgcgcgct cgttcgcgtc
     3421 ccctcctgga cttccccgat ggtcacgtcc tccggacgca cgcagaccaa gacgtcgcgc
     3481 gcaatctcgc tgccgacggc tgcgtcgatg agaacgccgg cctccgccgg cccaacctgc
     3541 acctgcacga cgccatccga gtgctccgtg accctgcctg agagaatcgt ctcgatacca
     3601 acgaaggcgg caatctcggc atcgctcgga gccgtgaaca cctcctgggc gggcccaacc
     3661 tggcggacgc gcccgtcgat gagcaccgcg atccgatcgc cgaagcgaag ggcctcgccc
     3721 cggtcgtgcg tgacgaagag tgccgtgaca ccggtctcgc ggatgagccg gctcagcaga
     3781 tcggcgagcg attcgcggct cggcgcgtct aacgaggcaa agggctcgtc cagcagcagc
     3841 acctcgggtt ggagggcgaa ggcgcgcgcc aagttcacgc gctgtgcctc gccgccggag
     3901 agcgatcgcg ctacccggtc cgccaagtgc aacacaccga acgcctcgag ccaccgatcc
     3961 tcccttgctc ggcgttcgcg cagcccccga aggcgcaacc ccagcgagac gttggcccgc
     4021 acggatcggt cgaggagcaa cggctcctgg aatacgacag ccatccgccg gcgatacttc
     4081 cgttcgctgc cgcgaaccga aacgccatcg aagcgaagct cgcccgcctg cggccgctcg
     4141 agcagcgccg ccgtgtgcag aagggtgctc ttgccggacc cgttcgggcc catgacggcg
     4201 aggacttcgc cttcgcgcag cgccagcgag gggacatcaa gggtgaatcg cccgcgccgc
     4261 agtcgcacac cacgcagctc gagcttggcg gggcgatcgt cgagctcgcc cgtcataacg
     4321 agcgtcgttg ctgctggagg acggtgagca ggatgacgat cgtgaacata atggcgagga
     4381 ggatgatgcc caaggcgagc gcaagttcga agttgccctt ctgcgtctcg aggacggccg
     4441 ccgtcgtcag aacgcgcgtc tcgccggaga tgtttccgcc cacctgcatc gatgcgccga
     4501 cttccgagat gacggcgccg aagcctgcca tc
//
LOCUS       DATMBB010000385         3921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_290213, whole genome shotgun sequence.
ACCESSION   DATMBB010000385 DATMBB010000000
VERSION     DATMBB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290213"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..470)
                     /locus_tag="VNL92_08195"
     CDS             complement(9..470)
                     /locus_tag="VNL92_08195"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWO94730.1"
                     /translation="MTRRRAGPRAPIERALSAGGVVYRRNETGDIEVAICGRTSDGLW
                     GLPKGTPDPGETIEQTALREVAEETGLDVEIEREIGAIDYWFVSAGGVRVHKTVRHFL
                     MRAIGGSIDRHDHEYDEVRWVPIDEAIGKMTHQNEADILRKVADTLRARTL"
     gene            complement(467..1402)
                     /locus_tag="VNL92_08200"
     CDS             complement(467..1402)
                     /locus_tag="VNL92_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWO94731.1"
                     /translation="MTIAELLLQLSDDDIEQGESKLGEFSHLSPEELQELRRVWPEMP
                     PERRLRLIERMAELNSSRIDFYFDDVFTLALDDDLPAIRIAAIGGLFEHEGRDIIGPL
                     IRALREDDDLGVRAASALALGRFAELAEANRLRMADAKAVEVALADAVRDEAEATEVR
                     ACAVQSIGARSEPWVHDLIEEAYFSGNYRMKLGAVGAMAASCDPAWLPALLDDLDDDD
                     PEMRFEAAMALGSIADEEAAPHLERLMEDDDEDVRLASVAALGGIASTNGDGQALEIL
                     RSHLADDDEDLRNWVETAIREALNFNDPFRLELDR"
     gene            complement(1424..2749)
                     /locus_tag="VNL92_08205"
     CDS             complement(1424..2749)
                     /locus_tag="VNL92_08205"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWO94732.1"
                     /translation="MSSDELAERVLGAARRSADQTEVFVLEADETPVRFEANRLKQIS
                     SRRTSGLALRVVKNGRIGFASSTAPGDVENLVAMALDVAQFGAEAKFDVAEAAEYPPV
                     DVCDEAVEAVSAETMVQLGQTVIDRIRADAAELLCEASVHRVVQRIRLLTSGGADASY
                     KRTIFAVHAEGTLVRGTDMLFLGDGEASCRLMRSGHTIAEAILQQLAWARETAPAVTT
                     KRMPVVFTPQGFASTFLAPLASAFSGRSVLQGSSPIGHRKGEAVYDHQLSIVDDATIA
                     FRPSSRSFDAEGVASRRVPLIDRGVVANFLYDRQTAGLAGVESTGHGERSLSTLPSIS
                     PATLMVEPGPCTFEEMLSDIDEGIVVEVLMGATQGNVQGGDFSGNVLLGYKVERGKIV
                     GRVKNTMVAGNAHEALANIVAIGSEPRWVGGSIYLPAIAFAELSVSTVG"
     gene            complement(2756..>3921)
                     /locus_tag="VNL92_08210"
     CDS             complement(2756..>3921)
                     /locus_tag="VNL92_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWO94733.1"
                     /translation="LRASVAAAVSQAHLAASGEPSQFAPIEPAVATLAPAVGEDPSAV
                     PLAEKKRILDEYNEIVWSTPSIATSSLRYGDSSRRITFANSDGAYIEQSRIDVTLGLS
                     VMARSNGDVQQAGFSKGALNDFAFVRQQSDEVRAAAERAVAFLHAEAAPGGEYTVVLD
                     PVLAGVFVHEAFGHLSESDHIYEDERMREIMTLGRRFGGPHLNIVDGAAIPGLRGSFA
                     YDDEGTPAQRTDLVREGVLVGRLHSRETAAKMGEQPTGNARALDYRFPPIVRMTNTLI
                     EPGDVSLEDILADIDDGLYVKNWYGGTTSMEMFTFSAGEAYHIRKGRIEELVRPVVLT
                     GNLFETLQNLDAVGDDLDMNQGGGCGKMGQMPLPVSNGSPHIRIRRCLIGSKG"
BASE COUNT          683 a         1327 c         1233 g          678 t
ORIGIN      
        1 gagcgtgctc acagcgttct cgccctcaag gtgtccgcca ccttccggag gatgtcggcc
       61 tcgttctgat gcgtcatctt gccgatcgcc tcgtcgatgg gaacccagcg gacctcgtca
      121 tactcgtggt cgtgccggtc gatggagccg ccaatggcgc gcataaggaa atgacgcacc
      181 gtcttgtgta cccgaacgcc ccccgcactc acgaaccaat agtcgatggc gccgatctcg
      241 cgctcaatct cgacatcgag cccggtttcc tcggcaacct cccgcagggc ggtctgctcg
      301 atggtttcgc cgggatcggg tgtacccttg ggcaaccccc agaggccatc gctcgttcgg
      361 ccgcagatcg ccacttcgat gtcccccgtc tcgttgcggc gatagacgac gcctccagcg
      421 gagagagccc gctcgatggg cgcgcgcggg cccgccctgc ggcgcgtcac ctgtcgagct
      481 cgaggcgaaa tgggtcgttg aagttgagcg cctcccggat ggccgtctcc acccagttgc
      541 gcaagtcctc atcgtcgtcg gcaagatgcg agcgcaggat ctcgagcgct tgtccgtcac
      601 cattcgtgga agcgatgccg ccgagggccg caacgctggc gagccgcacg tcctcatcgt
      661 catcctccat cagacgctcg aggtggggcg ccgcttcctc gtcggcgatg gagccgaggg
      721 ccatcgccgc ttcgaagcgc atctccggat cgtcgtcgtc gagatcatcg aggagtgcgg
      781 gtagccaggc cgggtcacag ctcgcggcca tggcgcctac ggcacccagc ttcatccgat
      841 agttgccgga gaagtaggct tcttcgatga gatcgtggac ccacggctca ctgcgagctc
      901 cgatagactg aacggcgcag gcccgcactt cggtggcctc cgcctcgtcg cgaacggcgt
      961 cggcgagcgc aacctccacc gccttcgcat ccgccatccg cagccgattc gcttccgcga
     1021 gctccgcgaa tcggcccagg gcgagcgccg atgcggccct cactcccaag tcatcgtctt
     1081 cgcggagggc acggatgagc gggccgatga tgtcgcgacc ttcgtgctcg aaaaggccac
     1141 cgatcgcagc gatgcggatc gctgggaggt cgtcgtcgag cgccagggta aacacgtcgt
     1201 cgaaatagaa gtcgatgcgg gagctgttga gctcggccat ccgttcgatt aaccgcagcc
     1261 gccgctccgg gggcatctca ggccacacgc ggcggagttc ctgtaactcc tccggcgaaa
     1321 gatgcgaaaa ctcgccgagc ttggattcgc cttgctcgat gtcgtcgtca ctgagttgca
     1381 gcagcaattc tgcgatggtc atggcaagag tcctctgggc gtctcagccg acggtcgata
     1441 cgctgagctc ggcgaacgct atcgcgggaa gatagataga cccgccgacc cagcgtggtt
     1501 cggaaccgat agcgacgatg ttggccaacg cttcgtgggc gttaccggcc accatcgtgt
     1561 tcttgacccg ccctacgatc ttaccgcgtt ccaccttata cccaagcagg acattccccg
     1621 aaaaatcgcc tccttgcacg ttgccctgcg tcgcgcccat cagcacctcg acgacgatgc
     1681 cctcgtcgat atcggacagc atctcctcga acgtgcaggg tcctggctcg accatgaggg
     1741 tggccggcga gatgctcggc agggtcgaaa gcgagcgctc accatggccg gtgctctcaa
     1801 cgccggcgag ccccgccgtc tgccggtcat agaggaagtt cgcgacgaca ccgcggtcga
     1861 tgagcgggac gcgccggctc gccacgccct cagcgtcgaa gctccggctc gaggggcgga
     1921 aggcgatcgt cgcgtcgtcg acgatggaga gctggtggtc gtacacggcc tcacctttgc
     1981 gatggccgat cggcgacgat ccctgaagca cagaacgacc cgaaaacgcc gacgccagcg
     2041 gggcgaggaa cgtactggca aacccctgcg gcgtgaaaac gaccggcatg cgtttcgtcg
     2101 tgaccgcagg cgccgtttcg cgcgcccaag ccagctgttg caggatggcc tccgcgatcg
     2161 tgtggccgct gcgcatcagg cgacacgagg cttcgccgtc gccgaggaag agcatgtccg
     2221 tgccgcgcac cagcgtgccc tcagcgtgca cagcgaagat ggttcgcttg tacgaggcat
     2281 cggcgccccc cgaggtcagc agacgaatcc gctgcacgac acggtgcacc gatgcctcgc
     2341 acagcaactc cgcggcgtcg gcacggatgc gatcgatgac cgtttgccca agctggacca
     2401 tggtttcggc cgagacggcc tcgacagcct cgtcgcacac atcgacaggc gggtactccg
     2461 ctgcttccgc gacgtcgaac ttggcttcgg cgccaaactg agcgacatcg agggccatgg
     2521 caacgagatt ctccacatcg ccgggcgcag tggacgaagc gaacccaatg cgaccgttct
     2581 ttacgacgcg caacgcgaga ccgctcgtcc gccgggacga gatctgcttg aggcgattcg
     2641 cctcgaaccg aaccggagtc tcgtccgcct cgagcacgaa gacctcggtt tggtcggcgg
     2701 agcgcctcgc agcgccgagc actcgttccg ctagttcatc gctactcacg tgacgctagc
     2761 ctttgctgcc gatgaggcag cgccggatgc ggatgtgcgg gctgccattc gaaacgggga
     2821 gcggcatctg ccccatcttg ccgcacccgc ctccctggtt catatcgagg tcgtccccca
     2881 ccgcgtcgag gttctggagc gtctcgaaca agttccccgt cagcacgacg ggacgaacca
     2941 gctcttcgat gcgccccttc cgaatgtggt aggcctcgcc ggcggagaac gtgaacatct
     3001 ccatgctcgt ggtgccgccg taccagttct tcacgtagag cccatcgtcg atgtcggcga
     3061 gtatatcctc gagcgagacg tcacccggct cgatgagcgt gttcgtcata cggacaatgg
     3121 gcgggaagcg gtagtccaga gcacgagcgt tccccgtcgg ttgctcgccc atctttgccg
     3181 ccgtctcccg cgaatgcagc cgcccaacga gcacgccctc acgcacgagg tccgtgcgct
     3241 gggccggcgt cccttcgtcg tcataggcga agctgccgcg caaaccggga atcgccgcgc
     3301 catccacgat gttgaggtga ggcccgccga aacggcgacc cagcgtcatg atctcgcgca
     3361 tccgctcgtc ctcatagatg tggtccgact cgctcaggtg cccgaaggcc tcgtgcacga
     3421 acactcccgc gaggacaggg tcgagcacga cggtatattc gccacccggc gccgcctccg
     3481 catggaggaa ggcgaccgca cgctccgctg cggcgcgcac ctcgtcggac tgctgacgca
     3541 cgaacgcgaa gtcgttgagt gcgcccttgc tgaagcccgc ctgctgcaca tcgccgttgg
     3601 agcgcgccat caccgatagg cccagcgtca cgtcgattcg ggactgctcg atatacgccc
     3661 catcactgtt ggcgaacgtg atgcggcgac tcgaatcgcc gtaacgcagg gacgatgtcg
     3721 cgatggacgg cgtggaccag acgatctcgt tgtactcgtc gaggatgcgc ttcttctccg
     3781 ccagcggcac ggcagagggg tcttcgccga cagccggcgc aagcgtcgct acagcaggct
     3841 cgatcggggc gaactgactc ggttcgccgc tggcggcgag gtgggcctgg gacacggcgg
     3901 cggcgacgct tgcccggagg t
//
LOCUS       DATMBB010000386         6166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_317635, whole genome shotgun sequence.
ACCESSION   DATMBB010000386 DATMBB010000000
VERSION     DATMBB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317635"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VNL92_08215"
     CDS             <1..1441
                     /locus_tag="VNL92_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWO94734.1"
                     /translation="FGLAIRAVVDSETMSALVGTNTSLVAASSWAMGAVLAGLAGILI
                     SPILNLSAINFALLVVASFAAVVIARLNSLTLAFAGALLLGLIQQVSVKYLPTEGVLA
                     SGFRPSVPFFVMAVFLLLYSFGGWGREPSHDERHHATAEPTSAPEPARGWLVRIGYPV
                     AGAIVVLGLPAILDSFWMGIVVSGLALAVIFLSYTLVTGEAGIVSLCQVTFAGIGAVA
                     AAQLSTEYDWPVALAMLGGGVAAVPFGIVVAGLAVRLEGLYLALATLAFALLMDNLVF
                     PIERFSQHASGVAMDRPAIGGYAFDTDVRYYYLLLAIFGALALFITSLRRSTTGLLWG
                     ALRSAETAAATLGYSIVRSKLTAFALSAFIAGLGGSLLAGYTLRAHPPSFNALIGLTW
                     LAVVVMWGVRGSMGAMAAGLMFMVLPQLATDYLPDELHQMPPLFFGLGAIALAREPRG
                     IVVLLANGLRSVRRVLSGRESPPALAQEG"
     gene            1447..3102
                     /locus_tag="VNL92_08220"
     CDS             1447..3102
                     /locus_tag="VNL92_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWO94735.1"
                     /translation="MSRIAPLGRSDTPSRAGIPAIEARRVSVHFGGLVALSDVDFAAQ
                     PGSITGLVGPNGAGKTTLFGALSGLLRPSAGEVLLRGARVTQESPQARARRGLARTFQ
                     RLELFGDLTVRQHLQLGYRARRAGSILWADLAGLGQRLVDRAEGEVVAELLADLGLRH
                     VADVPAATLPLGTGRLVEVARALAADPRVVLLDEPSSGLDVTETSELADVLLRAKQER
                     GVTFVLVEHNLDLVLRLSDVVYVLDFGRMIASGAPQAIASDPNVHTAYLGDTLATRVR
                     HPDGGETSDAATISPATGRDDSIGEEGAEVRTEFPLLRIEEVSARYGKVVALDSVTLD
                     IPEGKVLALLGANGAGKTTLARLISGLMRPASGRILFDGHDLRRMPAHRIARLGIAHV
                     PEGRGIFPGLSVGENLTLSLRASAKTRAERRASLDRVYTFFPVLAERRRQRAGTLSGG
                     EQQMLALARVLAVPPRLLVADELSMGLAPRLIDLVFAALEEARRSGVTVLLVEQFADR
                     ALGFADEAAILRLGRIVWQGPARQASGHVLAQYLGATFDGPGA"
     gene            3223..3540
                     /locus_tag="VNL92_08225"
     CDS             3223..3540
                     /locus_tag="VNL92_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWO94736.1"
                     /translation="MDERVRQLGDLTIDGATGDVWVNGRALDLSALEFRLLEFLASRP
                     SGTVSRDELIRALWTDDSPGNGRKLNLLVYRLRRKLEGTSSWRVRAMRRRGYMLCPAV
                     EDA"
     gene            3623..4048
                     /locus_tag="VNL92_08230"
     CDS             3623..4048
                     /locus_tag="VNL92_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94737.1"
                     /translation="MPSQYVATDSRTGLAIQVTGEFPDDTGERVRIARTVNLFTKLLA
                     TILITEGREQRELRFRALETQLETADALARGDMDEVRRLARETLRQMGLTDEQLREAT
                     QQLRDEIERMTGRPLEGELDWLFESQEGGAEEPPDSSED"
     gene            complement(4313..5122)
                     /gene="dapB"
                     /locus_tag="VNL92_08235"
     CDS             complement(4313..5122)
                     /gene="dapB"
                     /locus_tag="VNL92_08235"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWO94738.1"
                     /translation="MTDEPEPSLRIVVSGSGKMGREVLAAVYREQGLTPVGVLEKFAA
                     EDRIHLPDGESIPMSNDAAALIDLLRPDVIIDFTNAEWTCAFAPMAVESGVRLVIGTT
                     GLPAGWVDEMDHRCRERKIGAVVSSNFALGAVLMMHVARVVAPFFDHAEIIELHHDEK
                     VDAPSGTALQTVRGMLEARGRPFVHPPTERQTLAGTRGGVEDGATIHSVRLPGLVAHQ
                     EVIFGGLGQTLTIRHDTTGRECYMPGIVMAARAVMQKVGVVRGLEELMGLR"
     gene            complement(5405..6166)
                     /locus_tag="VNL92_08240"
                     /pseudo
     CDS             complement(5405..6166)
                     /locus_tag="VNL92_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
BASE COUNT          916 a         2062 c         2032 g         1156 t
ORIGIN      
        1 gttcgggctc gccatccgtg cagtcgtcga ctcggagacg atgagtgcac ttgtcgggac
       61 caacacatcg ctcgttgccg cttcctcgtg ggcgatgggg gcggtcctgg cggggttggc
      121 cggcatcctc atctcgccca tcctgaatct gtctgccatc aacttcgcgc ttctcgtcgt
      181 ggcgtcgttc gcggccgtcg tgatagcgcg gctcaatagt ctgacgctgg ccttcgccgg
      241 tgcgctgctg ctcggcctca tccagcaggt cagcgtcaag taccttccga ccgagggagt
      301 gctggcgagc ggcttccgtc ccagcgtgcc gttcttcgtc atggccgtgt tcctgctcct
      361 ctacagtttc gggggctggg gacgggaacc gtcccacgac gagcggcatc acgcgacggc
      421 cgagccgaca tcggcgccgg agccggcccg cggttggctg gtccgcatcg gctatcccgt
      481 tgcaggtgcc atcgttgtgc tcggcctgcc cgcgattctc gacagcttct ggatgggaat
      541 tgtcgtctcc gggctggccc tcgcggtcat ctttctctcc tacaccctcg tcacgggcga
      601 ggccgggatc gtgtccctct gccaggtgac cttcgcaggc atcggggcgg tagcggctgc
      661 tcaactctcg accgagtacg actggcccgt tgcccttgcc atgctcggcg gcggggtcgc
      721 agccgtaccg ttcggcatcg tggtcgccgg tctcgcggtg aggctcgagg gtctctacct
      781 ggccctcgca actctcgcgt tcgccctgct catggacaac ctcgtgtttc ccatcgagcg
      841 cttctcgcag cacgccagcg gtgtcgcgat ggaccggccc gccatcggcg gctacgcgtt
      901 cgataccgac gttcgctact attacctgct gcttgccatc ttcggggcgc tcgcgctgtt
      961 catcactagc ctgcggcgtt cgacgacggg gctgctttgg ggggcgctgc gctccgccga
     1021 gacagcggcg gcgaccttgg gctacagcat cgtccggtcg aagctcacgg cgttcgccct
     1081 gagcgccttc attgctgggc tgggcggcag cttgctggcc ggctacaccc tccgcgccca
     1141 tccgccgagc ttcaatgcgc tcatcggcct cacatggctc gccgtggtgg tgatgtgggg
     1201 cgtccgcggc agcatgggcg ccatggcggc tggtctcatg ttcatggtgc tgccgcagct
     1261 cgccaccgat tacctcccgg acgagttgca tcagatgccg ccgctcttct tcgggctggg
     1321 cgccatcgcc ctggctcggg agccgcgcgg catcgtcgtg ttgctggcga atggcctccg
     1381 ctccgtgcgc cgtgtgctca gtggccggga aagcccgccg gcgctggccc aggagggttg
     1441 acatcgatgt cccgcattgc ccccctcggc cgtagcgaca ccccgtcgcg ggccggcata
     1501 ccggccatcg aggctcgccg cgtcagcgtc catttcggcg ggctcgtggc cctctccgac
     1561 gtcgactttg cggcgcagcc cggctccatc acggggctgg tcgggcccaa cggcgccggg
     1621 aagacgacgc tgttcggcgc gctctcgggt cttctccgtc cttccgcggg cgaggtactt
     1681 ctccgtggag cacgagtaac gcaggagagt ccccaggcgc gggcccgccg cggattggca
     1741 cgaacattcc agcgtctcga gctcttcggc gacctcacgg tgcggcagca tctgcagctt
     1801 ggctacaggg cccgccgggc cggctcgatc ctctgggccg atcttgccgg cctagggcag
     1861 cgacttgtcg accgagctga gggagaggtt gttgctgaac tcctcgccga cctcggcctc
     1921 aggcacgtcg ccgatgtacc ggctgccacg cttcctctcg gcaccgggcg actcgttgaa
     1981 gtggcgcggg cgctcgccgc cgatccgcgt gtcgtcctgc tcgacgaacc atcttccggt
     2041 ctcgacgtga cggagacatc ggagctcgca gacgtgctgc tgcgcgcgaa gcaggagcgc
     2101 ggcgttacct tcgtcctcgt cgagcacaat ctcgacctcg tgttgcggct cagcgatgtg
     2161 gtttatgtgc tcgatttcgg ccggatgatc gcctcggggg cgccgcaggc catcgcctcc
     2221 gatccgaacg tgcataccgc ctaccttggt gacacgctcg cgacgagggt gcgccatccc
     2281 gacggcgggg agacgagcga cgctgcgacc atctcgccgg ccaccggccg cgatgactcg
     2341 attggcgaag aaggcgcgga ggttcgcacc gaatttccct tgttgcgcat cgaggaggta
     2401 tccgcgcgct acgggaaggt tgtggcgtta gacagcgtga cgctcgacat cccggaaggc
     2461 aaggtactgg ccctgctggg agcaaacggg gccgggaaaa caaccctcgc gcgtctcatc
     2521 tcgggcctta tgcggccggc gagtggccgc atcctcttcg atggacatga tctgcggcga
     2581 atgccagccc atcgcattgc gaggctcgga atcgcgcatg ttcccgaagg tcgcggcatc
     2641 ttccccgggc tcagcgtggg cgagaacctg acgttgtcgt tacgtgcgtc ggccaagaca
     2701 agggcggagc ggcgcgcatc tctcgaccgc gtgtacacgt tcttccccgt cctcgccgag
     2761 cgaaggcgtc agcgggcggg cacgctctcg ggcggcgagc agcagatgct ggcgctcgcg
     2821 cgggtgctcg cagtgccgcc gcgccttctt gtcgccgacg agctatcgat gggcttggcg
     2881 ccccgcctca tcgacctcgt gttcgccgca ctcgaagagg cgcgccgatc tggcgtcacg
     2941 gtgcttctgg tcgagcagtt cgccgaccgc gcgctcgggt ttgccgatga ggcagccatc
     3001 ctccggctcg gccgcattgt atggcaagga ccggcgcgcc aggcgagcgg ccatgtgctg
     3061 gctcagtacc ttggcgcgac gtttgatgga ccgggcgcat gatgcatgtt tgttaatggc
     3121 gacgaaacac gtgggaaatc aacgtgaaac ggccgctatc cacactaacg gcgagtctag
     3181 tcggctccac gggcagggac ggcgcatcgc agcatgcgag caatggacga gcgcgtacgg
     3241 cagctgggcg acctcacgat cgacggagcg actggcgatg tctgggtcaa cggccgcgca
     3301 ctcgacctgt cggcgctgga gttccgcctc ctcgagtttc ttgccagccg gccatccggt
     3361 acggtttccc gcgacgagct gattcgggcg ctgtggacgg acgacagtcc gggcaacggc
     3421 cgcaagctca atctcctcgt ctatcggctg cgtcgtaagc tggagggcac aagctcctgg
     3481 cgggtgcgag ccatgcggcg gcggggctac atgttatgcc cggccgtcga ggatgcgtag
     3541 accgacgcat cttccacact catcacgggc gacagtaggc aggatgttgc tcgcgggcct
     3601 acactggtcg gtggagcgag cgatgccgag ccagtacgta gcgaccgata gccgcacggg
     3661 cctcgcgatt caggtgacgg gcgagttccc tgatgatacc ggcgaacgcg tgcgcatcgc
     3721 gcggaccgtg aacctgttca cgaagctgct tgcgacgata ctcatcaccg aagggcgcga
     3781 gcagcgcgag ctccgcttca gggccttgga gacgcaactg gagacggccg atgccctcgc
     3841 ccgcggcgat atggacgagg tgcgccggct cgcgcgcgag acgctgcggc aaatggggct
     3901 cacggacgag cagctccgcg aggccacaca gcagctgcgc gacgagatcg aacggatgac
     3961 gggccggccg ctcgagggcg agctcgactg gctcttcgaa tcgcaggaag gcggggccga
     4021 ggagcctcct gacagctcgg aggattgaga ccggggggca agcccgcttc ccccgctgcc
     4081 tcggtcagcc accgctttcc ttgacgaaac tacctttctc tcttgacatc gcgttaatcc
     4141 gcgctccacg tgcgtggact actgtagctt cgagaagtct aggctcggcc gaggtgcggc
     4201 gatgcaggga aagaagtggg ccatcgtacg tggcgtcatc gatatcggag ggctcatcgc
     4261 cttcatggta gcggtcgagg cgctcgctcg cctggcgtaa acgggcccat cgttaccgta
     4321 ggcccatcag ctcctcgagt ccccggacga caccaacctt ctgcatgaca gctcgcgccg
     4381 ccatcacgat gccgggcatg tagcactccc tgccggtcgt gtcgtgtcgt atcgtcagcg
     4441 tctgtccaag gccgccgaag atgacctcct gatgtgcgac gaggcctggc agtcgaacgg
     4501 aatggatcgt ggcgccatcc tccacgccgc cacgcgtgcc cgccagggtt tgtcgctccg
     4561 tcggtgggtg cacgaacggc cgtccgcgcg cctccaacat cccgcgcacc gtctgcagcg
     4621 cggtccccga aggagcgtca actttctcgt catggtgcag ctcgatgatc tcggcgtggt
     4681 cgaagaacgg cgcgaccacg cgtgctacgt gcatcatgag gacggcgccg agggcaaagt
     4741 tcgacgagac gaccgcgccg atcttgcgct cgcggcagcg atggtccatc tcgtccaccc
     4801 agccggccgg cagtcctgtc gtcccgatga cgaggcgcac gccggactcg acggccatgg
     4861 gcgcgaacgc gcacgtccac tctgcattcg tgaagtcgat gatgacgtcg ggccgcaaca
     4921 ggtcgatgag tgcggcggca tcgttgctca tggggatgct ctcgccgtcc ggtagatgga
     4981 tgcgatcttc cgcggcgaac ttttcgagta cgccgacggg cgtcagaccc tgctcgcggt
     5041 agaccgccgc gagcacctcg cggcccatct tcccgctgcc gctcaccacg atgcgcaggc
     5101 ttggctctgg ttcgtcagtc atatgccgcc gctcctcggg gaatcgaaaa gggcctcgat
     5161 gggcccttct tcgttcacgc ggcgattgta gcgcattccc ctattcgtgg cgccggcgcc
     5221 gccggctcgg ctcgtcgccg ccggcgtgag gtgcaggcgt actgcgtggc gcccctcctc
     5281 cgttgaagct cgggccacga cgctcgcccg ggcgttggaa tcgccctgcg cccgtgtcgg
     5341 gtccacgtgg ccccctcggg ccggcaccgc caccgccccg agcgggagcg cgtgcgagca
     5401 cgtcctcggg ctcttcgttc tggagtacag cgcggcgcga gaggttgatc cgccccatcg
     5461 agtcgatctc gctgaccatg accgttacct catcgccggc ctgaacgacg tcctcgacgc
     5521 ggttcactcg ctccgtatcg agctcagaga tgtggacgag gccttccttg ccgggcagaa
     5581 tctcgacgaa tgccccgaag gacatgaggc gcgtaacctt gcccgtgtaa atctggccga
     5641 tctcgacctc tttcgtgagt gcctcgatca tctggatggc gcgacgtgcc tgcgcttcgt
     5701 tcgtagccgc gatgacgacc atgccgtcat cctcgatgtc gatctggcac tttgtctcct
     5761 cgaccatggc gcggatgacg cgcccgcccg gaccgatgac ggcgccgatc ttatcgggct
     5821 cgatctgaat ctggtaaatg cgaggggcgt acggcgacag gttgtcgcgg ggcgcgctga
     5881 tggtctccat gagcctttcg aggatgaaca atcggccctc acgcgcctgt tcaagggcca
     5941 ctcgcatgat ttcgagcgta atccccttga ccttgatgtc catctggagg gccgtgatac
     6001 cgttctgcgt cccggcgacc ttgaaatcca tgtcgccgta gtggtcttcc atgccgacga
     6061 tgtcggtcag gactcgatac tggccgccgt ccccgagcac aaggcccatg gcaacgccgg
     6121 cgacgggctg cgagatgggc acacccgcgt ccatgagcgc gagggt
//
LOCUS       DATMBB010000387         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_12580, whole genome shotgun sequence.
ACCESSION   DATMBB010000387 DATMBB010000000
VERSION     DATMBB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12580"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..900
                     /locus_tag="VNL92_08245"
     CDS             136..900
                     /locus_tag="VNL92_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWO94739.1"
                     /translation="MPLLSRALVVLPTYNERESIESVLRGLLSHPHLDALVIDDNSPD
                     GTAEVASALALRLPGRVHVLRRHAKEGLGSAYRDGFVAALALDYERFVQMDADGSHAP
                     DAVPRLVAALDDADLVLGSRYCEGGQSPGWSLWRRGISRLAGELAGIALHLPQADLTS
                     GFKAWRRQTLEAIEPATLRSRGFAIQIETTYRAHRAGFSIVELPVEFRPRASGESKFT
                     FGICAEAAYQVGRLRLQRWQPSRSAAPTGRLPATGR"
     gene            complement(922..>2233)
                     /locus_tag="VNL92_08250"
     CDS             complement(922..>2233)
                     /locus_tag="VNL92_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWO94740.1"
                     /translation="PVLLASAAKVIGVAPLADALADLAPSPLEARPIEAESGALSADP
                     AGPTVLRVFKTTADPYVGRLTYFRVVSGTVTSDSHLMNANTGRDERLSQLFVVCGKSQ
                     EPAPRLHAGDIGAVAKLAETGTGDTLCAKGHETKLPPIALPSPVYDVAVSPKTKADLD
                     KLGAALTRIVEEDPTLRVHRDATTGETVLSGLGETHVDVAIERMRRKFGVDVEAHTPR
                     VAYRETIRTKANVEYKHKKQTGGHGQYGHVFLELEPLPRGSGVEFGDRVVGGVVPKNY
                     IPAVEKGVMEAVQEGAIANCPLVDMKVTLYDGSHHPVDSSEMAFKIAASQAMKKGVLQ
                     ANPALLEPVAALHIQVPEEFVGDVISDLNGKRGQVLGMTPNGSMTTIEAMAPLAEVQR
                     YAIDLRSLTQGRASFSMELDHYDEVPQQLAQKVIDEAHREKVAS"
BASE COUNT          349 a          765 c          709 g          410 t
ORIGIN      
        1 cctcgtacgc tgcgttgttt acatctactc taccctcacg aggcacgttt cgccatcgag
       61 acgcatcgac gacaaccgtg tcgcgggcat ggtaggatca agcccgagac gggagcgatc
      121 ggaaggaggc gggccgtgcc cctcctgtcg cgtgctctgg tcgtgctgcc gacctacaac
      181 gagcgcgaga gcatcgagtc ggtacttcgc ggattgctct cgcatccgca tctcgacgcg
      241 ctcgtcatcg acgacaactc cccagacggc acggcagagg ttgcctccgc ccttgccctg
      301 cgtctccccg gccgcgtcca cgtcctgcgg cggcacgcca aggaaggtct cgggtcggcc
      361 tatcgcgacg gcttcgttgc tgcgctcgcc ctcgactatg aacgctttgt gcaaatggat
      421 gcggacgggt cccatgcacc ggacgccgtg cctcgcctcg tggccgctct cgacgatgcc
      481 gacctcgtgc tcggaagccg ctactgcgag ggcgggcagt ctccaggatg gtccttgtgg
      541 cgacgcggca tctcccgcct ggcgggcgag ctcgccggaa tcgccctcca cctcccccag
      601 gccgacctca cgagcggatt caaggcttgg cgacgacaga ccctcgaggc gatagagccc
      661 gccaccctgc gttcacgcgg ctttgcaatc cagatcgaga cgacgtaccg cgcccatcgc
      721 gcaggcttca gtattgtcga actgcccgtg gagttccgtc cgcgggcgtc cggggaatcg
      781 aaattcacgt tcggtatctg cgcggaagct gcgtaccagg tcggccgcct gcggcttcag
      841 cgatggcagc cgtcccgaag cgcggcgcct acgggacgtc ttcccgccac cgggcggtga
      901 cgagccgaag acgcaatctg ctcagctcgc gactttctcc cggtgcgcct cgtcgatcac
      961 cttctgcgcc agctgttgcg ggacctcgtc gtagtgatcg agctccatcg agaagctcgc
     1021 gcggccctgt gtcagggatc gcagatcgat ggcgtagcgc tgcacctcgg cgagcggcgc
     1081 catcgcttcg atagtcgtca tcgagccgtt cggcgtcatc cccagcacct ggccgcgctt
     1141 gccgttgaga tcgctgatca cgtcgccgac gaactcctcc ggcacctgga tgtgcagggc
     1201 cgccaccggt tcgaggagag cggggttggc ctggagtacg cccttcttca tcgcctgcga
     1261 ggcggcaatc ttgaatgcca tctccgagga atcgacgggg tggtggctac catcgtacag
     1321 cgtcaccttc atgtcgacga ggggacagtt cgcgatcgcc ccctcctgga cagcctccat
     1381 gacccccttc tccaccgctg gaatgtagtt cttcgggacg acgccgccga caacccgatc
     1441 gccgaactcg acaccggacc ctcggggaag cggctcgagc tcgaggaaga cgtggccgta
     1501 ctgaccgtgg ccgccggtct gcttcttgtg cttgtattcg acgttcgcct tcgtgcggat
     1561 ggtctcgcgg taggccactc ttggcgtatg cgcctccacg tcgacgccga actttcgtcg
     1621 catgcgctca atcgccacgt ccacatgggt ttcgccgagg cccgagagca ccgtctcgcc
     1681 cgttgtggcg tcgcgatgaa cccgcaacgt cggatcctct tcgactatcc gggtgagggc
     1741 cgcaccaagc ttgtcgaggt cggccttcgt cttggggctc actgcgacat cgtagaccgg
     1801 cgaaggcagc gcgatgggtg gcagtttcgt ttcgtgaccc tttgcgcata gcgtatcgcc
     1861 cgtgcccgtc tcggccagct tcgcgaccgc accgatatcg ccggcatgga gccgaggggc
     1921 cggctcctgg ctcttgccac acacgacgaa gagctgcgaa agccgctcat cccgtcccgt
     1981 attcgcgttc atcaggtgcg agtccgacgt gacggtcccc gacacgacgc ggaagtacgt
     2041 gaggcggccg acgtaggggt ccgccgtcgt cttgaacacg cgcaggacgg taggccctgc
     2101 cggatccgcg gacaacgctc cggattctgc ctcaatcggg cgtgcctcga gcggggaggg
     2161 ggcgaggtcg gcaagcgcgt ccgcgagcgg cgcgacgccg atgaccttgg ctgccgaggc
     2221 aagcagcacg ggc
//
LOCUS       DATMBB010000388         3760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Dehalococcoidia bacterium isolate SMAG_U13341
            k141_577765, whole genome shotgun sequence.
ACCESSION   DATMBB010000388 DATMBB010000000
VERSION     DATMBB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942751
            Sequence Read Archive: SRR7882662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Dehalococcoidia bacterium (soil metagenome)
  ORGANISM  Dehalococcoidia bacterium
            Bacteria; Chloroflexota; Dehalococcoidia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 13:38:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,655
            CDSs (total)                      :: 1,633
            Genes (coding)                    :: 1,617
            CDSs (with protein)               :: 1,617
            Genes (RNA)                       :: 22
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 21
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Dehalococcoidia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577765"
                     /isolate="SMAG_U13341"
                     /isolation_source="soil"
                     /db_xref="taxon:2026734"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.801167 N 76.3429 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VNL92_08255"
     CDS             <1..344
                     /locus_tag="VNL92_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWO94741.1"
                     /translation="RPKIGVLTGAALLAVVLLLVNRGQVLIGDTTFMKSMIPHHSIAI
                     NNARKATIRDPRVRRLADEIIASQVREIREMQLLLEDIERNGRRGGVALPARPALLTP
                     DMLQKVREAVQ"
     gene            complement(577..1917)
                     /locus_tag="VNL92_08260"
     CDS             complement(577..1917)
                     /locus_tag="VNL92_08260"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HWO94742.1"
                     /translation="MQPSELRTYTWARHSSAAAVAVLTVFGVALVAHAHDFWLVPDAF
                     RIAEGGYVEVRGQTSSRFPTSESAVALDRVADARLISASGSERIGEMSRRGTSLFLRH
                     RPTIPGQRVVAVTLRPRSIRESAAGFRRYLELEGAPEALARIEREGLLRGRDSVTRRY
                     AKYAKTVVEVGSGGARAFSAIARHPLEFVPRGDPARLVAGDTAAIQLLYRGQPLSGAA
                     IHAGAVSRRGRPGTTQAGSGEASLDSIVAPDVHLTTDASGVLRLPINAAGLWNVRTIH
                     VVQADQGSGADWDTHWATLVFEAGEHGAGAERSGASRAGTPRSDSSEVANVVARYHEA
                     LAVGDSATALSLLASDAVVLESGEMETREEYRSHHLPADIQFARAVRSERSPLRVTLR
                     GDVAWVSSTSTAQGQYRGRAVNSAGAELMVLTREADGWRIRAIHWSSRTRRTPG"
     gene            2084..2662
                     /locus_tag="VNL92_08265"
     CDS             2084..2662
                     /locus_tag="VNL92_08265"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWO94743.1"
                     /translation="MSVVLAAFTLVAACARSETDTSDTAAGAADAGMTGMDRSSAAAN
                     RGPSTDADHEFLRMMSDHHEGLIEMASAAMNKGSTAQIQGDAHQLHTKQEQEQKEMVD
                     MVRNAYGENLEPAIMPSNRAMNDTLQQKSGVEYDRTFYRMVVQHHREGIRMMDQFRPR
                     VQRAEVRQMLDRMRSEQQREIQELEAKIARVN"
     gene            complement(2742..3347)
                     /locus_tag="VNL92_08270"
     CDS             complement(2742..3347)
                     /locus_tag="VNL92_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HWO94744.1"
                     /translation="MRTHQSALLLGLAAILGLGACSSDQALGPAETGVDAIASQTRIA
                     SEPAPDRSTARYEIDFMQDMIDHHAMAVMMAEMCVEKAVHEELRRLCEDIIAAQSAEI
                     QQMQGWLQEWYGISYAPEMTPGAMKQMEKLAALSGAEFEIAFMEMMIKHHAKAVKEGE
                     RCVERVYHEELRELCENIIATQTAEIVLMESWLCEWYGNCK"
     gene            3529..>3760
                     /locus_tag="VNL92_08275"
     CDS             3529..>3760
                     /locus_tag="VNL92_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWO94745.1"
                     /translation="MPAHRASKGFSVVVLVTLALYAFVGVVLLQPWLVGGLLGHDTAG
                     HIGQHFRQPHHRVHDLTFSFLMGTAGVGTLVQL"
BASE COUNT          628 a         1290 c         1221 g          621 t
ORIGIN      
        1 cccgccccaa gatcggtgtc ctcacaggtg cagccttgct cgccgtcgtc ctcctgctcg
       61 tgaaccgtgg ccaggtgctg attggcgaca cgacgttcat gaagtcgatg atcccgcacc
      121 actcgatcgc catcaacaac gcccggaagg cgaccatccg cgatccgagg gtccggaggt
      181 tggcggacga gatcatcgcc tcgcaggtac gggagatccg cgagatgcag ctgctgctcg
      241 aggacatcga acgcaatggc cgccgcggag gtgtcgccct cccggcccgt ccggcactgc
      301 tcacgcccga catgctgcag aaggtccgtg aggcggttca gtagaccgat ccttccggat
      361 cggccggtct cggccgggag cgagcatccc tgcttagcgt gatcctcctg cgcttcgctc
      421 gcgtgggcgg actcgaggag cgccgcggac ccggtgcgcc gggcatggtg ccgatcgtcg
      481 ccggacgaga atctctgatt acatgcgccc gctgatgtca tcggccgccg cctgtgcatc
      541 cgcgcacacg aactcgggat gcgcgacccg gcccggctaa ccgggagtgc gtcgggttcg
      601 cgacgaccag tggatcgcgc ggatcctcca cccatccgcc tcgcgcgtca gcaccatgag
      661 ctcggcaccc gccgagttga cggcccgccc gcggtactgg ccctgcgctg tgctcgtgga
      721 gctgacccac gccacgtcgc cgcggagcgt gacgcgaagg gggctccgtt cgctacgcac
      781 ggcacgagcg aactggatgt cggccggcag gtgatgcgag cggtattcct cgcgcgtctc
      841 catctcgcca ctctcgagga cgaccgcatc ggaagcgagg agtgacagcg cggtcgcgct
      901 gtctccgacg gcgagcgcct cgtgatatcg tgcgacgacg ttcgcgacct cgctcgagtc
      961 ggaccgggga gtgcccgccc tcgatgcgcc cgagcgctcg gccccggcgc cgtgctcacc
     1021 ggcctcgaat acgagcgtcg cccagtgcgt gtcccagtcc gccccggagc cctgatcggc
     1081 ctggacgacg tggatcgttc gtacgttcca gagtcccgct gcgttgatcg ggaggcgcag
     1141 cacgcccgat gcgtcagtcg tgaggtgcac atccggcgcg acgatggaat cgagcgaggc
     1201 ctcgccggat cccgcctgcg tcgtcccagg tcgaccgcgc cggctgaccg caccggcgtg
     1261 gatcgcggcg ccggatagcg gctgcccccg atagagaagc tggatcgctg ccgtatcgcc
     1321 ggctacgagt ctcgcagggt ccccgcgtgg aacgaactcg agcgggtgcc gagcgatggc
     1381 tgagaacgcg cgtgctccgc cggagcccac ctcgacgacg gtcttggcgt acttcgcgta
     1441 tcggcgcgtc acgctgtcgc ggccgcggag cagtccctcg cgctcgatcc gcgcaagcgc
     1501 ctcgggcgcg ccttcgagct ccagatagcg gcggaagccc gcggccgact ctcggatgga
     1561 gcgcgggcgc agcgtcactg cgacgacgcg ctgtcccggt atggtcggtc ggtggcggag
     1621 gaaaagcgac gtaccgcgcc gcgacatctc gccgatgcgc tcggagccgg aagcgctgat
     1681 gagtcgcgcg tcggcgacac gatccagcgc cacggcagat tcgctcgtcg ggaatcggga
     1741 gctcgtctgt ccacgcacct cgacgtaccc gccttccgcg atccggaacg cgtcgggtac
     1801 gagccagaag tcgtgggcgt gggcgaccag cgccacgccg aacactgtga gcacggccac
     1861 ggcagcagcc gaggaatgac gggcccacgt gtacgtccga agttcgctcg gttgcacgct
     1921 aacctccacc ggaagtgccc tgtcctctcc cgtagcggcc gcccgccgag caagacaggg
     1981 catgttttat gcccgataaa atcggttccc ggcgttcgca agtcaacctg tctcgaccta
     2041 ccgcgaaacc cctatgcctc gactgtctgc tcctggcgtt cgcttgagcg tcgtcctcgc
     2101 cgctttcacc ctcgttgccg cgtgcgcccg atcggaaacc gataccagcg acaccgccgc
     2161 cggagcggcc gacgcgggga tgaccgggat ggaccgctcg agcgccgctg cgaaccgtgg
     2221 cccctcgacg gacgccgacc acgagttcct gcgcatgatg agcgaccacc acgaggggct
     2281 catcgagatg gcgtcggccg ccatgaacaa gggcagcacg gcccagattc aaggcgacgc
     2341 ccaccagttg cacaccaagc aggagcagga gcagaaggag atggtggaca tggtcaggaa
     2401 cgcctacggc gagaacctcg agccggcgat catgcccagc aacagggcga tgaacgacac
     2461 gctgcagcag aagtccggtg tggaatacga ccggaccttc taccgcatgg tcgtgcagca
     2521 tcaccgcgag gggatccgga tgatggacca gttccggcca cgcgtgcagc gcgccgaggt
     2581 acgacagatg ctcgacagga tgcggtcgga acagcagcgg gagattcagg agctggaggc
     2641 gaagatcgct cgcgtgaatt gattaactcg ccacctcaac tgcaaacatc gaggggccgg
     2701 gagttcccgg cccctcgacg catccgaagg ccgtctttgg cctacttaca gttgccgtac
     2761 cactcgcaga gccaggactc catcagcacg atctcggccg tctgcgtcgc gatgatgttc
     2821 tcacagagct cgcgaagctc ttcgtgatag acacgctcca cgcaccgctc gccttccttc
     2881 accgccttcg cgtggtgctt gatcatcatc tccatgaaag cgatctcgaa ctccgcgccg
     2941 gacaacgcgg cgagcttctc catctgcttc atggcacctg gggtcatctc gggtgcgtag
     3001 ctgataccgt accactcctg gagccacccc tgcatctgct ggatctccgc gctctgcgcg
     3061 gcgatgatgt cctcgcagag tcgcctcagc tcctcgtgca ccgccttctc gacacacatc
     3121 tcggccatca tgacggccat ggcgtggtga tcgatcatgt cctgcatgaa gtcgatctcg
     3181 tacctggcgg tcgaccggtc cggagcgggt tcgctcgcga tgcgggtctg cgacgcgatg
     3241 gcgtcgaccc cggtctcagc agggccgagc gcctgatcgc tggagcaggc gccgagaccg
     3301 aggatcgctg ctaggccgag cagaagggcg ctctggtgtg ttcgcatgac gacgcctcca
     3361 aaggagtgta cgacgggcct gaccgtgcac aactacggcg cacggcggcg ggactcgaga
     3421 ggccgcaatg gccatgccgc gctttatggc gcataaaggg catccgcgta acgccttccg
     3481 cttggctcag tcgggcgtta ctcgtttcag caacaggagg tccagcggat gcccgctcat
     3541 cgcgcttcga aagggttctc ggtggtcgtg ctcgtgaccc tcgcgctgta cgcgttcgtc
     3601 ggtgtggtgc tgcttcagcc ctggctcgtc ggcgggctgc tcgggcacga caccgctggg
     3661 cacatcggcc agcacttccg gcaaccccac caccgcgtcc acgacctcac gttctcgttt
     3721 ctcatgggga cggccggagt cggaacgctg gtccagctcc
//
